LOCUS       DAMZKC010000001        52953 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.001, whole genome shotgun sequence.
ACCESSION   DAMZKC010000001 DAMZKC010000000
VERSION     DAMZKC010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 52953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52953
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.001"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            94..2166
                     /gene="lip2"
                     /locus_tag="POB55_000001"
     CDS             94..2166
                     /gene="lip2"
                     /locus_tag="POB55_000001"
                     /EC_number="3.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK domain-containing triacylglycerol lipase
                     Lip2/Geh"
                     /protein_id="HDI6499002.1"
                     /translation="MLRGQEERKYSIRKYSIGVVSVLAATMFVVSSHEAQASEKTPTN
                     AAVQKETLNQPGEQGNAITSHQMQSGKQLDDMHKENGKSGTVTEGKDTLQLSKYQSTQ
                     NSKTIRTQNDNQVKQDSERQGSKQSHQNNATNNTERQNDQVQNTHHAERNGSQSTTSQ
                     SNDVDKSQPSIPAQKVLPNHDKAAPTSTTPPSNDKTAPKSTKAQDATTDKHPNQQDTH
                     QPAHQIIDAKQDDTVRQSEQKPQVGDLSKHIDGQNSPEKPTDKNTDNKQLIKDALQAP
                     KTRSTTNAAADAKKVRPLKANQVQPLNKYPVVFVHGFLGLVGDNAPALYPNYWGGNKF
                     KVIEELRKQGYNVHQASVSAFGSNYDRAVELYYYIKGGRVDYGAAHAAKYGHERYGKT
                     YKGIMPNWEPGKKVHLVGHSMGGQTIRLMEEFLRNGNKEEIAYHKAHGGEISPLFTGG
                     HNNMVASITTLATPHNGSQAADKFGNTEAVRKIMFALNRFMGNKYSNIDLGLTQWGFK
                     QLPNESYIDYIKRVSKSKIWTSDDNAAYDLTLDGSAKLNNMTSMNPNITYTTYTGVSS
                     HTGPLGYENPDLGTFFLMDTTSRIIGHDAREEWRKNDGVVPVISSLHPSNQPFVNVTN
                     DEPATRRGIWQVKPIIQGWDHVDFIGVDFLDFKRKGAELANFYTGIINDLLRVEATES
                     KGTQLKAS"
     gene            complement(2408..3235)
                     /locus_tag="POB55_000002"
     CDS             complement(2408..3235)
                     /locus_tag="POB55_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDI6499003.1"
                     /translation="MTEIKDKVITKDAFALPYTIIKAKNQPTKGAIVYIHGGGLMFGK
                     ANDLSPQYIDILTEHYDLIQLSYRLLPEVSLDCIIEDVYASFDAIQSQYSNCPIFTFG
                     RSSGAYLSLLIARDRDIDGVIDFYGYSRINTEPFKTTNSYYAKIAQSVNETMIAQLTS
                     PTPVVQDQIAQRFLIYVYARGTGKWINMINIADYTDSKYNIAPDELKTLPPVFIAHCN
                     GDYDVPVEESEHIMNHVPHSTFERVNKNEHDFDRRPNDEAITIYRKVVDFLNAITMM"
     gene            complement(3308..4507)
                     /locus_tag="POB55_000003"
     CDS             complement(3308..4507)
                     /locus_tag="POB55_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HDI6499004.1"
                     /translation="MYRYKPLLQSIHLPNGIKISNRFVLSPMTVNTSTKEGYITKEDL
                     AYAARRSNSAGMQVTGAAYIEPYGQLFEYGFNIDHDACIPGLTNMASTMKQHGSLAII
                     QLAHAGRFSNQAILNFGKVYGPSPMTLHSPIEHDVIAMSHEKINSIIQQYRDATLRAI
                     KAGFDGVEISIAQRLLIQTFFSTFSNKRTDHYGADSLKNRARLCLEVMRAVQEVIDKE
                     APDNFILGFRATPEETRGSDLGYTIDEFNQLIDWVMDVSNIQYLAIASWGRHIYQNTS
                     RTPGDHFGRPVNQIVYEHLAGRIPLIASGGINSPESALDALQHADMVGMSSPFVTEPD
                     FVHKLAEQRPHDINLEFSMADLEDLAIPHAAFKDIVKMMDYGEGLKKHTRDALRQLEQ
                     NYHDSSS"
     gene            4603..4695
                     /locus_tag="POB55_000004"
     CDS             4603..4695
                     /locus_tag="POB55_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499005.1"
                     /translation="MESIFKIKLMNGICRSENMNMKKKNKGEKI"
     gene            4809..5810
                     /locus_tag="POB55_000005"
     CDS             4809..5810
                     /locus_tag="POB55_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HDI6499006.1"
                     /translation="MVKLSVLDYALIDEGKDAQKALQDSVTLAKLADRLGFKRIWFTE
                     HHNVPAFACSSPELLMMHTLAQTNHIRVGSGGVMLPHYRPYKIAEHFRMMAALYPNRI
                     DLGIGNNPGTTMVKQALDGINPTYDSYDESISLLRDYLTIKDKPSAHTLGVQPHIDHF
                     PEMWLLSSSATSAKIAAELGIGLSVGTFLLPDINAIHAAKDNIDIYKKHFQASTIKMD
                     AKVMASVFVIVADNEAEVAALQHALDVWLLGKLQFAEFEDFPSVDTAQKYKLNDRDKE
                     MIQAHQARIIAGTQEKVKAQLDDFIATFEVDEVLVAPLIPGIEQRCKTLKLLAEIYL"
     gene            5844..6176
                     /locus_tag="POB55_000006"
     CDS             5844..6176
                     /locus_tag="POB55_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HDI6499007.1"
                     /translation="MKKLANYLWVEKVGDLYVFSMTPELQDDIGTVGYVEFVSPDEVK
                     VDDEIVSIEASKTVIDVQTPLSGTIIERNTKAEEEPTILNSEKPEENWLFKLDDVDKE
                     AFLALPEA"
     gene            6177..6977
                     /locus_tag="POB55_000007"
     CDS             6177..6977
                     /locus_tag="POB55_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HDI6499008.1"
                     /translation="METLKSNKARLEYLINDMHRERNDNDVLVMPSSFEDLWELYRGL
                     ANVRPALPVSDEYLAVQDAMLSDLNRQHVTDLKDLKPIKGDNIFVWQGDITTLKIDAI
                     VNAANSRFLGCMQANHDCIDNIIHTKAGVQVRLDCAEIIRQQGRNEGVGKAKITRGYN
                     LPAKYIIHTVGPQIRRLPVSKMNQDLLAKCYLSCLKLADQHSLNHVAFCCISTGVFAF
                     PQDEAAEIAVRTVESYLKETNSTLKVVFNVFTDKDLQLYKEAFNRDAE"
     gene            6967..7911
                     /locus_tag="POB55_000008"
     CDS             6967..7911
                     /locus_tag="POB55_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacetylase"
                     /protein_id="HDI6499009.1"
                     /translation="MQSSKWNAMSLLMDDKTKQAEVLRTAIDEADAIVIGIGAGMSAS
                     DGFTYVGERFTENFPDFIEKYRFFDMLQASLHPYGSWQEYWAFESRFITLNYLDQPVG
                     QSYLALKSLVEGKQYHIITTNADNAFDVADYDMTHVFHIQGEYILQQCSQHCHAQTYR
                     NDDLIRKMVVAQQDMLIPWEMIPRCPKCDAPMEVNKRKAEVGMVEDAEFHAQLHRYNA
                     FLEQHQDDKVLYLEIGIGYTTPQFVKHPFQRMTRKNENALYMTMNKKAYRIPNSIQER
                     TIHLTEDISTLITTALRNDSTTQNNNIGETKDVLNRTD"
     gene            7889..8911
                     /locus_tag="POB55_000009"
     CDS             7889..8911
                     /locus_tag="POB55_000009"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HDI6499010.1"
                     /translation="MYLIEPIRNGEYITDGAIALAMQVYVNQHIFLDEDILFPYYCDP
                     KVEIGRFQNTAIEVNQDYIDKHSIQVVRRDTGGGAVYVDKGAVNMCCILEQDTSIYGD
                     FQRFYQPAIKALHTLGATDVIQSGRNDLTLNGKKVSGAAMTLMNNRIYGGYSLLLDVN
                     YEAMDKVLKPNRKKIASKGIKSVRARVGHLREALDEKYRGITIEEFKNLMVTQILGID
                     DIKEAKRYELTDADWEAIDELVDKKYKNWDWNYGKSPKYEYNRSERLSSGTVDITISV
                     EQNRIADCRIFGDFFGQGDIKDVEEALQGTKMTREDLTHQLKQLDIVYYFGNVTVGSL
                     VEMILS"
     gene            9227..10252
                     /locus_tag="POB55_000010"
     CDS             9227..10252
                     /locus_tag="POB55_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde reductase"
                     /protein_id="HDI6499011.1"
                     /translation="MNNKVLVTGGTGFVGMRIISRLLEQGYEVQTTIRDLSKADKVIK
                     TMQDNGISTERLMFVEADLSQDEHWDEAMKDCKYVLSVASPVFFGKTDDAEVMAKPAI
                     EGIQRILRAAEHAGVKRVVMTANFGAVGFSNKDKNSITNESHWTNEDEPGLSVYEKSK
                     LLAEKAAWDFVENENTTVEFATINPVAIFGPSLDAHVSGSFHLLENLLNGSMKRVPQI
                     PLNVVDVRDVAELHILAMTNEQANGKRFIATADGQINLLEIAKLIKEKRPEIAQKVST
                     KKLPDFVLSLGAKFNHQAKEGKLLLDMNRNVSNERAKILLGWEPIATQKEAILAAVDS
                     MAKYHLI"
     gene            complement(10345..11691)
                     /locus_tag="POB55_000011"
     CDS             complement(10345..11691)
                     /locus_tag="POB55_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDI6499012.1"
                     /translation="MQAILNFIVDILSQPAILVALIAFIGLIVQKKPAATITSGTIKT
                     ILGFLILSAGADVVVRSLEPFGKIFQHAFGVQGIVPNNEAIVSLALKDFGTTAALIMV
                     CGMIVNILIARFTNLKYIFLTGHHTFYMAAFLAIILTVSHIKGWLTIVIGALVLGLIM
                     AVLPALLQPTMRKITGNDQVALGHFGSISYFAAGAVGQLFKGKSKSTEEIKFPKGLSF
                     LRESTISISITMALLYFIACLFAGVSYVHESISNGQNFIVFSLIQGVTFAAGVFIILT
                     GVRLILAEIVPAFKGISEKLVPNSKPALDCPIVFPYAQNAVLIGFFVSFITGVIGMFI
                     LFLFGGVVILPGVVAHFFLGATAAVFGNARGGIKGAVAGAALNGILITFLPLLFLPFL
                     GELGGAATTFSDTDFLTVGIVFGNAVKYMGLFGAILFIIIVGATAILLKGRQKPQQ"
     gene            complement(11706..11990)
                     /locus_tag="POB55_000012"
     CDS             complement(11706..11990)
                     /locus_tag="POB55_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDI6499013.1"
                     /translation="MKILVVCGHGLGSSFMVEMNAQEALRQLNAPSDIEVEHSDIMTA
                     SPEMADLFICGRDLAENAERLGDVLVLDNILDKAELQQKLSEKLQQLNMI"
     gene            complement(11992..12435)
                     /locus_tag="POB55_000013"
     CDS             complement(11992..12435)
                     /locus_tag="POB55_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDI6499014.1"
                     /translation="MSLDILSTTRIIVKEQVNDWTEAITIASQPLLQEQIIEQGYVQA
                     MIDSVNELGPYIVIAPEIAIAHARPNNDVHQVGLSLLKLNQHVAFCDEDHYASLIFVL
                     SAIDNHSHLSVLQNLATVLGDNQTVQQLLTATNAQDIKNILKEHD"
     gene            complement(12440..14395)
                     /locus_tag="POB55_000014"
     CDS             complement(12440..14395)
                     /locus_tag="POB55_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDI6499015.1"
                     /translation="MLSYRQIEILYLLIHEQTFIPINTIANQLGVSPRTIQYDIAYIE
                     QYAETYHYQVSRNKAAGIKVTTAHATLLNELEHNLTNQIHFSKDERLTHIALKLFETT
                     DPVSTKQLAQDVNVSRRTIADDIKMIQAQLDQYHLKLNYVHNKGFNIIGEEDHYRKAY
                     AHFIHQYMKQAAPFIEADIFNSESIALVRRAIIKTLNSENYHLVQSAIDGLIYHILIA
                     IQRLNENFSFDIPINEIDKWRHTNQYAIASKMIENLERSCNVTFPESEIIFITLHLLG
                     SKMTEHTASSITFEYHDLSQNIHELITCVSQELGIDMSKDNKLHTSLITHIKPAIHRI
                     KYDMLQPNPLRQEVMRRYPQIIEAVSKHISPIEQDAAIRFNEDELTYITIHFASSIER
                     VATHKQSMIKVVLLCGSGIGTSQLLKSKLNHLYPEFHIWDAYSIYQLEESRLLQDNID
                     YVISTVPCEISAVPVIHVDPFINQQSRQKLNQIINDSREQRVMKMATDGKSLADLLPE
                     HRIIINKQPLSIESAIAVAVQPLINDGIVYSNYTAAILKQFEQFGSYMVISPHIALIH
                     AGTDYVQNGVGFALTYFTEGIIFGSKANDPVHLVITLATDHPNAHLKALGQLSECLSN
                     DLYRQDFLDGNIFKIKQHIALTMTKEA"
     gene            14607..15026
                     /gene="mepR"
                     /locus_tag="POB55_000015"
     CDS             14607..15026
                     /gene="mepR"
                     /locus_tag="POB55_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor MepR"
                     /protein_id="HDI6499016.1"
                     /translation="MEFTYSYLFRMISHEMKQKADQKLEQFDITNEQGHTLGYLYAHQ
                     QDGLTQNDIAKALQRTGPTVSNLLRNLERKKLIYRYVDAQDTRRKNIGLTTSGIKLVE
                     AFTSIFDEMEQTLVSQLSEEENEQMKANLTKMLSSLQ"
     gene            15133..16488
                     /gene="mepA"
                     /locus_tag="POB55_000016"
     CDS             15133..16488
                     /gene="mepA"
                     /locus_tag="POB55_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498905.1"
                     /note="MepA (multidrug export protein A), as described
                     originally in Staphylococcus aureus, is a MATE transporter
                     shown to be over-expressed after culturing in the presence
                     of tigecycline, owing to mutation of its repressor MepR,
                     and then to be one of several factors contributing to
                     elevated tigecycline resistance; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MepA"
                     /protein_id="HDI6499017.1"
                     /translation="MKDEQLYYFEKSPVFKAMMHFSLPMMIGTLLSVIYGILNIYFIG
                     FLEDSHMISAISLTLPVFAILMGLGNLFGVGAGTYISRLLGAKDYSKSKFVSSFSIYG
                     GIALGLIVILVTLPFSDQIAAILGARGETLALTSNYLKVMFLSAPFVILFFILEQFAR
                     AIGAPMISMIGMLASVGLNIILDPILIFGFDLNVVGAALGTAISNVAAALFFIVYFMK
                     NSDVVSVNIKLAKPNKEMLSEIFKIGIPAFLMSILMGFTGLVLNLFLAHYGNFAIASY
                     GISFRLVQFPELIIMGLCEGVVPLIAYNFMANKGRMKDVIKAVIMSIGVIFVVCMIAV
                     FTIGHHMVGLFTTDQDIVEMATFILKVTMTSLLLNGIGFLFTGMLQATGQGRGATIMA
                     ILQGAIIIPVLFIMNALFGLTGVIWSLLIAESLCALAAMLIVYLLRDRLTVDTSELIE
                     G"
     gene            16595..17035
                     /locus_tag="POB55_000017"
     CDS             16595..17035
                     /locus_tag="POB55_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MepB family protein"
                     /protein_id="HDI6499018.1"
                     /translation="MYKSKILLKYIFSEEPEVKDLTEEKYNQDYEALTFSFKEETYQS
                     RLAKKTPTKAGYFVTCWTKDENNCNQPYSKEAFADYLMIIVIDEELSGYFLFPRELLV
                     EKGILTTFEHKGKMAFRVYPKWCNQLNKTAGQTQKWQCKYFFEY"
     gene            complement(17118..18476)
                     /gene="glpT"
                     /locus_tag="POB55_000018"
     CDS             complement(17118..18476)
                     /gene="glpT"
                     /locus_tag="POB55_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HDI6499019.1"
                     /translation="MNFLKPAKHIKPLPENQIDDTYKRLRLQVFLGIFIGYAGYYLLR
                     KNFSLAMPALQEQGFTKAELGFALSAVSIAYGFSKFFMGTVSDRSNARIFLVLGLALT
                     AIVNLLMGFVPFFTSGIGIMFVLLFLNGWFQGMGWPPSGRVLVHWFSVSERGSKTALW
                     NVAHNVGGGIMAPIAAWGITTTAFINFGYLKGFEGVFIYPALLALIIAAISYVLIRDT
                     PQSQGLPPIEIYKNDFATSDKKTLETELTTKEILFKYVLNNKWVWAIAFANIFVYFVR
                     YGVLDWAPVYLSEEKHFDLKASGWAYFLYEWAGIPGTLLCGYISDKLFKGRRGPAGFF
                     FMLGVTVFVLIYWLNPPGNAWLDNVSLIAIGFLIYGPVMLIGLQALDYVPKKAAGTAA
                     GLTGLFGYLFGAVMANIVLGAVVDKFGWDVGFILLTAISVFAMLSFILTWNKVGQETV
                     HH"
     gene            18788..19714
                     /locus_tag="POB55_000019"
     CDS             18788..19714
                     /locus_tag="POB55_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDI6499020.1"
                     /translation="MNIVGHHHISMYTKDAKRNKDFYTNVLGLRLVEKSVNQDNPSMY
                     HLFYGDEVGTAGTILSFFEIPNAGHKQPGTETIYRFSLLVPNQAALHYFEKRLDNNGI
                     TSERLYYLGQEGVVFKDEDDLEIILLVNDSFEVPHQWQHNVYSEIPQAYQILGIGPVE
                     LRVRNAARTVEFLENVLGYRKRDNKSFDVLTLAPQGLYSDFVVIEQQGQRERPGRGYI
                     HHIAINTPQMSDLDAIYKKLQQQTQSNSGIIDRYFFKSLYYRHNSIMYEFATEAPGFT
                     IDTPLEQLGSQLNLPDFLEAEREQIESKLHEI"
     gene            19728..20789
                     /locus_tag="POB55_000020"
     CDS             19728..20789
                     /locus_tag="POB55_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HDI6499021.1"
                     /translation="MAKLEMNKNTPLEFGLYSLGDHLLNPLKGEKVSYEQRINEIIEA
                     SKLADEAGIDVFAVGESHQEHFTTQAPTVVLGAIAQATKHIKVSSSSTIISATDPVRV
                     FEDFATLDLISHGRAEIVAGRASRTGIFDLFGYDLKDYDELFEEKLGLLLELNKTERI
                     TWSGKYRPELRNMKIFPRPIDNTLPIWRAVGGPPASAIKAGKQGVPMMITTLGGPAMN
                     FKGSIDAYRQAATEAGFDASPKSLPVSIASLFYTAETTQDAMREFYPHLNTGMSFIRG
                     VGYPKQQFANSPDYREALMVGSPQQIIEKILYQHELYGHQRFMAQLDFGGVPFENVMK
                     NIELIGNDIIPAIKKHLSK"
     gene            20803..21369
                     /locus_tag="POB55_000021"
     CDS             20803..21369
                     /locus_tag="POB55_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDI6499022.1"
                     /translation="MNIVLLSGSTVGSKTRIAMDDLKNELEVINEGHQIELMDLRELE
                     LEFSVGKNYLDTTGDVYKLTTSLMQADVIFIGFPIFQASIPGALKNVFDLLPVNAFRD
                     KVIGLVATAGSSKHYLIPEMHLKPILSYMKAHTMQTYVFIEEKDFSNQQIVNDDVVFR
                     LKALAQSTMRTAKVQQQVFEEENNQYDF"
     gene            complement(21429..22424)
                     /locus_tag="POB55_000022"
     CDS             complement(21429..22424)
                     /locus_tag="POB55_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HDI6499023.1"
                     /translation="MASLKNKHFMIGLTLTFIVALFSFLAAKLPILDKVGALTIAILI
                     AILYRHFRGYPEQYSSGITFSSKYLLRFAIILYGLKLNIFDIIGQGSRLLAIDVGVVI
                     FSIVMMLFVNKLLHGDKNIALLLGVGTGVCGAAAIAAVAPIFKSREKDTAISIGIIAL
                     IGTIFSLIYTAIYAIFSMTTNVYGAWSGVSLHEIAHVVLAGGFGGSDALKIALLGKLG
                     RVFLLIPLTIVLILIMRFRSSESSSNGRISIPYFLIGFVIMALVNTYVTIPSALLNIL
                     NTVSTICLLMAMVALGLNVAFKDLKNRALKPLMTIIITSICLSSLAFIVVHWLYS"
     gene            22729..23274
                     /locus_tag="POB55_000023"
     CDS             22729..23274
                     /locus_tag="POB55_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6499024.1"
                     /translation="MFGMKVNEQIILKILEAHDTEALFNLVNRSRNSLREWLPWVDAT
                     EQPSDTRAFIKRGLLQFSDGNGFQCGIWYEGTLVGVIGLHEINRMHRKTSLGYYLDKQ
                     FEGHGIMTQAVEALIKYCFEELDLNRIEISAAVNNEKSRAIPERLGFTREGMLRDNEL
                     LNGIYSSSYIYSLLKSEYDQK"
     gene            23541..24395
                     /locus_tag="POB55_000024"
     CDS             23541..24395
                     /locus_tag="POB55_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499025.1"
                     /translation="MKKLTTLLLASTLLIAACGNDDSKKDDSKTSKKDDGVKAELKQA
                     TKAYDKYTDEQLNEFLKGTEKFVKAIENNDMAQAKALYPKVRMYYERSEPVAEAFGDL
                     DPKIDARLADMKEEKKEKEWSGYHKIEKALYEDKKIDDVTKKDAQQLLKDAKELHAKA
                     DTLDITPKLMLQGSVDLLNEVATSKITGEEEIYSHTDLYDFKANIEGAQKIYDLFKPI
                     LEKKDKKLSDDIQMNFDKVNQLLDKYKDNNGGYESFEKVSKKDRKAFADAVNALGEPL
                     SKMAVITE"
     gene            24392..25621
                     /gene="efeB"
                     /locus_tag="POB55_000025"
     CDS             24392..25621
                     /gene="efeB"
                     /locus_tag="POB55_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HDI6499026.1"
                     /translation="MTNYEQVNDSTQFSRRTFLKMLGIGGAGVAIGASGVGSMWSFKS
                     MFNTPEDPEKDAYEFYGKVQPGITTPTQKTCNFVALDLKSKDRDAIKAMFKKWTVMAD
                     RMMDGDKVGKPSNNPLMPPVDTGESIGLGASKLTITFGISKSLMKKIGLSSKIPDAFK
                     DLPHFPNDQLIDDYSDGDIMIQACSNDSQVSFHAVHNLVRPFRDIVKVRWAQSGFISA
                     KGKETSRNLMAFKDGTINPRKSNQLKDYVFIDDGWAKHGTYCVVRRIQIHIETWDRTA
                     LEEQEATFGRKRHSGAPLTGGKEFDEIDLKAKDSHGEYIIDKDAHTRLAKEANTSILR
                     RAFNYVDGTDDRTGNFETGLLFIAFQKATKQFIDIQNNLGSNDKLNEYITHRGSASFL
                     VLPGVSKGGYLGETLFD"
     gene            25602..27314
                     /locus_tag="POB55_000026"
     CDS             25602..27314
                     /locus_tag="POB55_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family iron permease"
                     /protein_id="HDI6499027.1"
                     /translation="MKHYLIKFVAMLITAAMVCSFGLLKSQAAKQQSISDVYSVITDA
                     KSALSNNSISNDNKQKAIEQVVSAVKKLSLEDNSEGNAVKSDVRKLEDAKANDNQKDT
                     LSQLTKSLIAYEEKLASKDAGSKIKLLQQQVDAKDAVMTKAIKDKNKAELESLNNSLN
                     QIWTSNETVIRNYDANQYGQIEVALLQLRIAIHKSPLDTAKVSHAWTTFKSNIDHVDK
                     KSDTSAKDQYHVSQLNDELEKAIKAIDDNQLSDADAALTHFIEIWPYVEGQIQTKDGA
                     LYTKIEDKIPYYQSVLDEHNKAHVKDGLVDLNNQIKEVVGHSYSFVDVMIIFLREGLE
                     VLLIVMTLTTMTRNVKDKKGTASVIGGAIAGLVLSIILAITFVETLGNSGILRESMEA
                     GLGIVAVILMFIVGVWMHKRSNAKRWNDMIKNMYANAISNGNLVLLATIGLISVLREG
                     VEVIIFYMGMIGELATKDFIIGIALAIVILIIFALLFRFIVRLIPIFYIFRVLSIFIF
                     IMGFKMLGVSIQKLQLLGAMPRHVIEGFPTINWLGFYPTYEPLIAQAAYIMVVAILIF
                     KFKK"
     gene            complement(27406..28107)
                     /gene="tatC"
                     /locus_tag="POB55_000027"
     CDS             complement(27406..28107)
                     /gene="tatC"
                     /locus_tag="POB55_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006191819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HDI6499028.1"
                     /translation="MVHFSELRHRLVKILLSFVITVIVVYVSSFWWMTPFITYITRAH
                     VSLHAFSFTEMIQIYVMIIFFIAFCLISPVMFYQLWAFVAPGLHNNERQFIYKYSFFS
                     VLLFIFGVAFAFYVGFPMIIQFALKLSLTLNISPVIGFKAYLIELIRWLFTFGILFQL
                     PILFIGLAKFGLIDTTSLKHYRKYIYFACFVLASIIAPPDLTLNILLTLPLILLFEFS
                     TFIVKFTYRGKPPTH"
     gene            complement(28124..28348)
                     /locus_tag="POB55_000028"
     CDS             complement(28124..28348)
                     /locus_tag="POB55_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HDI6499029.1"
                     /translation="MIIMITNTFILGITGPTSLVVISIIALIIFGPKKLPQFGRAIGS
                     TLKEFKSATEDLDKESHDTPSKESKQQREQ"
     gene            complement(28447..28836)
                     /locus_tag="POB55_000029"
     CDS             complement(28447..28836)
                     /locus_tag="POB55_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HDI6499030.1"
                     /translation="MAFTLSAIQQAHQQFTGVDFPKLFKAFKDMGMTYNIVNIQDGTA
                     TYVHQSEDDIVTSSVKSTIPVAQKSNQSIVQDVLTRHQQGQTDFETFCDEMAEAGIYK
                     WHIDIQAGTCTYIDLQDQAVISELIPQ"
     gene            29076..29279
                     /locus_tag="POB55_000030"
     CDS             29076..29279
                     /locus_tag="POB55_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI6499031.1"
                     /translation="MRNRLKELRARDGLNQTQLAKLAGVSRQTISLIERNNFMPSVLT
                     AIKIARIFNETVETVFIIEEDEV"
     gene            29276..29989
                     /locus_tag="POB55_000031"
     CDS             29276..29989
                     /locus_tag="POB55_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HDI6499032.1"
                     /translation="MKILRYIGYLLLGGLVGGIIGGILGNFDGLGIENLTFATYTNVV
                     VISIVATIIIILVEAIVLMNQRRALKYKRLVDEEVDIDATDQYELLANRYVLNGSILS
                     VIQTIIAFVVLLIFVVGQAEANAMLFFLIPFFASAIFNTQFTLFNRKFDDRMPKIADK
                     NYTEKRLEILDEGERHIELIALFKTYAINLSILILAIIFIGSYSIATGINQSFSLLLI
                     IAIFIYNAFSYLLKRRRFY"
     gene            30014..30856
                     /locus_tag="POB55_000032"
     CDS             30014..30856
                     /locus_tag="POB55_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6499033.1"
                     /translation="MTTLLNVDRVNKQYKDSDFKLQDASLTISTNETVGLIGKNGSGK
                     STLINILVGNRHKDNGSIAFFGEEHAADDVEYKEHIGVVFDDLRVPNKLTIKDIDKVF
                     QSIYTTWNSQKFFDLIKYFELPRQTKIKTFSRGMRMKIALTIALSHDVKLLILDEATA
                     GMDVSGREEVMELLEDFVAQGGGILISSHISEDIEQLADKLVFMKDGRMILTEQKDIL
                     LAQYGIVTTGDKDVEITKHLIIASRLSKGKYQILVKDYAEIENAEPLKHIDDATKIIM
                     RGEV"
     gene            30856..31485
                     /locus_tag="POB55_000033"
     CDS             30856..31485
                     /locus_tag="POB55_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HDI6499034.1"
                     /translation="MKGMFLSSFYATRKQTYIYFIVAIIAAGYFAVFNPLMSSAMAGV
                     MLITPITDNIKHEKDSRWMYYVSTLPVKRSDYIKSYFAFYLILFGASLMIGLVVTTIV
                     TQSVMIGIMSGLMSFGIIGAYSIIFPLTFKFGAENSNVIMIVASILLLFSFVVFFFIY
                     GMVSGASALEFEKISTEGWLVVIVYAVIGIVITSVSYILSIKIFNKQEL"
     gene            complement(31875..33008)
                     /locus_tag="POB55_000034"
     CDS             complement(31875..33008)
                     /locus_tag="POB55_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HDI6499035.1"
                     /translation="MVNALTPKHLAEKRAGFHELFFDLIFVYAIQKIAHVILTTQNGS
                     ISADLFFKYIVMSLFLWLMWSHQTFFTNRFGQVTFKDVSFMMFNMFIMVFLSNSLYPD
                     FEKTFFPFFLCVAIMYLSIGLQYLLHIRTGLDYGDKRTCQAFASVAFVISFLSFLSLV
                     LPQSIHYIPGFLGVFIAATGLIPFQKYLVLSPVNMMHLVERFSLLTIIIFGEVLVGLA
                     SSSFSIDHFSYIYIFQFMILISLFGVYWIITENYINHKLSSIGFRLSYTHLLINIALG
                     VINAAIVFSNNNKLNDLFEINMMYISVLIFYIGLWLITPYFHNELTNAKYISSSLGIL
                     VVSYIISLIFKGHDQVMIISVSVATFCIMLIYFKNQRLRNKHE"
     gene            33547..34728
                     /locus_tag="POB55_000035"
     CDS             33547..34728
                     /locus_tag="POB55_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HDI6499036.1"
                     /translation="MTRVVLAAAYRTPIGVFGGAFKDVPAYDLGATLIEHIIKETGLN
                     PSEIDEVIIGNVLQAGQGQNPARIAAMKGGLPETVPAFTVNKVCGSGLKSIQLAYQSI
                     VTGENDIVLAGGMENMSQSPMLVNNSRFGFKMGHQSMVDSMVYDGLTDVFNQYHMGIT
                     AENLVEQYGISREEQDTFAVNSQQKAVRAQQNGEFDSEIVPVSIPQRKGEPILVTKDE
                     GVRENVSVEKLSRLRPAFKKDGTVTAGNASGINDGAAMMLVMSEDKAKELNIEPLAVL
                     DGFGSHGVDPSIMGIAPVGAVEKALKRSKKELSDIDVFELNEAFAAQSLAVDRELKLP
                     PEKVNVKGGAIALGHPIGASGARVLVTLLHQLNDEVETGLTSLCIGGGQAIAAVVSKY
                     K"
     gene            complement(34811..35563)
                     /locus_tag="POB55_000036"
     CDS             complement(34811..35563)
                     /locus_tag="POB55_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HDI6499037.1"
                     /translation="MTQYPLWQQLNTLKQATWVDLTHTFDPEIPRFSEFEKGEVSTLF
                     TVKDHGFYVQRWNIVTQYGTHIDAPIHFVENKRYLEDIDLKELVLPLIVLDFSTEVAN
                     NNDFIVTRAHIEAWEKEHGTIEPGTFVALRTDWSKRWPNIEKFENKDANGQQHAPGWG
                     LDALKYLIEERRVEAVGHETFDTDASVDVVKNGDLVGERYILGQDKYQVELLTNLDQL
                     PTRGAIIYAISPKPKDAPGFPVRAFAIKPSND"
     gene            complement(35606..37834)
                     /gene="metE"
                     /locus_tag="POB55_000037"
     CDS             complement(35606..37834)
                     /gene="metE"
                     /locus_tag="POB55_000037"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HDI6499038.1"
                     /translation="MTTIKTSNLGFPRLGRKREWKKAIESYWAKKISKEELDQTLTDL
                     HKENLLLQKYYHLDSIPVGDFSLYDHILDTSLLFNIIPERFQGRTIDDDLLFDIARGN
                     KDHVASALIKWFNTNYHYIVPEWDNVEPKVSRNVLLDRFKYAQSLNVNAHPVIVGPIT
                     FVKLSKGGHQTFEEKVKTLLPLYKEVFESLIDAGAEYIQVDEPILVTDDSESYENITR
                     EAYDYFEKAGVAKKLVIQTYFERAHLKFLSSLPVGGLGLDFVHDNGYNLKQIEAGDFD
                     KSKTLYAGIIDGRNVWASDIEAKKVLIDKLLAHTNELVIQPSSSLLHVPVSLDDETLD
                     TSVGEGLSFATEKLDELDALRRLFNQNDSVKYDKLKARYERFQNQSFKNLDYDFESVR
                     TSRQSPFAQRIEQQQKRLNLPDLPTTTIGSFPQSREVRKYRADWKNKRITDEAYETFL
                     KNEIARWIKIQEDIGLDVLVHGEFERNDMVEFFGEKLQGFLVTKFGWVQSYGSRAVKP
                     PIIYGDVKWTAPLTVDETVYAQSLTDKPVKGMLTGPVTILNWSFERVDLPRKVVQDQI
                     ALAINEEVLALEAAGIKVIQVDEPALREGLPLRSEYHEQYLKDAVLSFKLATSSVRDE
                     TQIHTHMCYSQFGQIIHAIHDLDADVISIETSRSHGDLIKDFEDINYDLGIGLGVYDI
                     HSPRIPTKEEITTAINRSLQQIDRSLFWVNPDCGLKTRKEEEVKDALTVLVNAVKAKR
                     QE"
     gene            complement(37831..39672)
                     /locus_tag="POB55_000038"
     CDS             complement(37831..39672)
                     /locus_tag="POB55_000038"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HDI6499039.1"
                     /translation="MSQFLTQLKDNVLVADGAIGTILYSEGLDTCPEAYNLSHPDKVE
                     RIHRSYIEAGADVIQTNTYGANFEKLKRFGLEDKVKAIHQAAVRIAKKAANKDTYILG
                     TVGGFRGIKQEDISLQTILYHTEIQIDTLIEEGVDALLFETYYDLEELTNVISRTRKK
                     YDIPIIAQLTASNTNYLVNGQAINEGLKQLVQCGANIVGLNCHHGPHHMQESFTHIEL
                     PEHAYLSCYPNASLLDIENSEFKYSDNAQYFGQVAQNLIHEGVRLIGGCCGTTPEHIK
                     FIKESIQTLKPVNDKKVIPIPTKALFNPSQNKVRQSLTSKVQERPTVIIELDTPKHLD
                     TDRFFENIAKLDKANVDAVTLADNSLATVRISNIAAASLIKQYYNIEPLVHITCRDRN
                     LIGLQSHLLGLSLIGVNEILAITGDPSKVGHLPGATNVYDVNSKGLTELALRFNQGIN
                     TDGDALKKRTHFNIAGAFNPNVRKLDGAVKRLEKKIESGMSYFITQPVYSKEKIIEIY
                     HATKHLNKPFFIGIMPIASYKNALFLHNEVPGIKMSDEILQQFEAVKDDNAKTRELSL
                     KLSKDLIDTVHEYFNGLYIITPFQNVEDSLELAAYSKSITAHKEAIL"
     gene            complement(39641..40801)
                     /locus_tag="POB55_000039"
     CDS             complement(39641..40801)
                     /locus_tag="POB55_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HDI6499040.1"
                     /translation="MTLSKETEVIFDWRRGVEYHSANPPLYDSSTFHQTSLGGDVKYD
                     YARSGNPNRELLEEKLARLEQGKFAFAFASGIAAISAVLLTFKSGDHVILPDDVYGGT
                     FRLTEQILNRFNIEFTTVDTTKLEQIEGAIQSNTKLIYIETPSNPCFKITDIKAVSKI
                     AEKHELLVAVDNTFMTPLGQSPLLLGADIVIHSATKFLSGHSDLIAGAVITNNEAISE
                     ALYLIQNGTGNMLSAQDSWTLAKHLKTFPIRFKQSVENAQKIVSFLIKQDEISEVYYP
                     GLTTAHLEQAKNGGAVIGFRLADESKAQQFVDALTLPLVSVSLGGVETILSHPATMSH
                     AALPEEVRQERGITFGLFRLSVGLEDPDELIADIKYALKEAFNESIPHTIER"
     gene            complement(40798..41901)
                     /locus_tag="POB55_000040"
     CDS             complement(40798..41901)
                     /locus_tag="POB55_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDI6499041.1"
                     /translation="MKDTQLAQITLTDDSTGAIANPIHLSTAYKHPKLGQSTGFDYTR
                     TKNPTRSTFETCFAKLEHGIASFATSSGMSAIQLICNLFKPHDEILVSFDLYGGTFRL
                     FEFYEQQYNIKFKYVDFTDYEQVEKEITDKTVALFIEPISNPQMIAIDVKPYYQLCKA
                     KGLLSIIDNTFLTPYLSTPLAEGADIVLHSATKYIGGHNDVLAGVVTVKDESLAQQLF
                     DFHNMTGATLSPIDSYLLLRGLKTLHLRIERAQSNARKLAKKCQSLQAIDEVLYSGQT
                     GMLSLRLNKAYSVAKLLENLDICIFAESLGGTETLVTFPYTQTHVDMPDAEKDKRGID
                     EYLIRLSLGVENYEDIERDIIQALDKAQIGEIV"
     misc_binding    complement(41967..42336)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            42565..43410
                     /locus_tag="POB55_000041"
     CDS             42565..43410
                     /locus_tag="POB55_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HDI6499042.1"
                     /translation="MSELSKSEDQRITKTKDEQIKQIDISDIKPNPYQPRKTFDENHL
                     NDLADSIKQYGILQPIVLRKTVQGYYIVVGERRFRASKIAGLKYVSAIIKDLTDEDMM
                     ELAVIENLQREDLNAIEEAESYQRLMTDLKITQQEVAKRLSKSRPYIANMLRLLHLPK
                     KIADMVKDGRLTSAHGRTLLAIKDEQQMLRLAKRVVKEKWSVRYLENHVNELKNVSSK
                     SETDKVDITKPKFIKQQERQLREQYGTKVDISIKKSVGKISFEFDSQEDFLRIIEQLN
                     RRYGK"
     gene            43567..44448
                     /locus_tag="POB55_000042"
     CDS             43567..44448
                     /locus_tag="POB55_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HDI6499043.1"
                     /translation="MNQVMNIISSLFEPLTKIETYENIATKIAMIVIYIIVALIVIKI
                     LNKMIEQGFKIQNKSKKSNKKRSKTLISLVQNVVKYIVWFIVITTILSKFGISVEGVI
                     ASAGVVGLAVGFGAQTIVKDVITGFFIIFESQFDVGDYVKINNGGTTVAEGTVKSIGL
                     RSTRINTISGELTILPNSSMGEITNYSITNGTAIVKIPVSVEENIDNVDKKLNKLFTS
                     LRSKYYLFVSDPVVIGIDAIEDTRVILRISAETIPGEGFAGARIIRKEVQKMFLQEGI
                     KTPQPIMTAYNHSENGV"
     gene            44478..44681
                     /locus_tag="POB55_000043"
     CDS             44478..44681
                     /locus_tag="POB55_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HDI6499044.1"
                     /translation="MASKYGINDIVEMKKQHACGTNRFKIIRMGADIRIKCENCQRSI
                     MIPRQTFDKKLKKIIESHDDTQR"
     gene            44693..45790
                     /gene="ychF"
                     /locus_tag="POB55_000044"
     CDS             44693..45790
                     /gene="ychF"
                     /locus_tag="POB55_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019469841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HDI6499045.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGALAANYPFATIDPNVGIV
                     EVPDARLLKLEEMVQPKKTLPTTFEFTDIAGIVKGASKGEGLGNKFLSHIREVDAICQ
                     VVRAFDDDNVTHVAGRVDPIDDIEVINMELVLADLESVEKRLPRIEKLARQKDKTAEM
                     EVRILTTIKEALENGKPARSIDFNEEDQKWVNQAQLLTSKKMLYIANVGEDEIGDDDN
                     DKVKAIREYAAQEDSEVIVISAKIEEEIATLDDEDKEMFLEDLGIEEPGLDRLIRTTY
                     ELLGLSTYFTAGVQEVRAWTFKQGMTAPQCAGIIHTDFERGFIRAEVTSYDDYVQYGG
                     ESGAKEAGRQRLEGKEYIMQDGDIVHFRFNV"
     gene            complement(45876..46067)
                     /locus_tag="POB55_000045"
     CDS             complement(45876..46067)
                     /locus_tag="POB55_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499046.1"
                     /translation="MNLNIDWSKDFQEFQEILNSGIHPEWLYCAKANLVLEPAYTGEG
                     KQFFSTQDIINASKIIPFF"
     gene            46311..46607
                     /locus_tag="POB55_000046"
     CDS             46311..46607
                     /locus_tag="POB55_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001831345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HDI6499047.1"
                     /translation="MRTYEVMYIVRPNIEEDAKKALVERFNGILATEGAEVLEAKDWG
                     KRRLAYEINDFKDGFYNIVRVKSDNNKATDEFQRLAKISDDIIRYMVIREDEDK"
     gene            46628..47131
                     /gene="ssb"
                     /locus_tag="POB55_000047"
     CDS             46628..47131
                     /gene="ssb"
                     /locus_tag="POB55_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDI6499048.1"
                     /translation="MLNRVVLVGRLTKDPEYRTTPSGVSVATFTLAVNRTFTNAQGER
                     EADFINCVVFRRQADNVNNYLSKGSLAGVDGRLQSRNYENQEGRRVFVTEVVCDSVQF
                     LEPKNAQQNGGQRQQNEFQDYGQGFGGQQSGQNNSYNNSSNTKQSDNPFANANGPIDI
                     SDDDLPF"
     gene            47183..47425
                     /gene="rpsR"
                     /locus_tag="POB55_000048"
     CDS             47183..47425
                     /gene="rpsR"
                     /locus_tag="POB55_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002484306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HDI6499049.1"
                     /translation="MAGGPRRGGRRRKKVCYFTANGITHIDYKDTELLKRFISERGKI
                     LPRRVTGTSAKYQRMLTTAIKRSRHMALLPYVKEEQ"
     gene            complement(47691..48629)
                     /locus_tag="POB55_000049"
     CDS             complement(47691..48629)
                     /locus_tag="POB55_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HDI6499050.1"
                     /translation="MLNFDEQIAKLKQMNIFFNIIDTEKANEILRKNNHFFKLAYFRK
                     NFEKKNGGYFIEFAYLSDLATIDMKLRYTMLHLTLDIEHSLKCLVLKLITENNQEDGY
                     KIIDEFLCIDKSYSNSNFDTNSRTPEEVMETKIKNKNEIFKHMNKRGQLPEKLNKYYQ
                     NPPAWVCIEFMQLGQFVSFLNFYYKKYNDEELRVANILMPLVKNIRNKSAHNQPIIAN
                     LNYDSRSPQYLFEKGNNIGISRNMFGIKNFIDTFATLELHNQVCSNAIIQARYHDLDQ
                     LQKRYKRNESYYNNALAIKRFFIALDKIIDFNRPKV"
     gene            complement(48680..49382)
                     /locus_tag="POB55_000050"
                     /pseudo
     CDS             complement(48680..49382)
                     /locus_tag="POB55_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000270135.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            49588..50199
                     /locus_tag="POB55_000051"
     CDS             49588..50199
                     /locus_tag="POB55_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HDI6499051.1"
                     /translation="MFKKNDSKNSILLKSILSLGIIYGGTFGIYPKADASTQNSPSVQ
                     DKQFQKVEEVPNNSEKALVKKLYDRYSQNTINGKSNKSRNWVYSERPLNENQVRINLE
                     GTYRVADRVYTPKRNITLNKEVVTLKELDHIIRFAHISYGLYMGEHLPKGNIVINTKD
                     GGKYTLESHKELQKDRENVKINTADIKNVTFKLVKSVNDIEQV"
     gene            50568..50969
                     /locus_tag="POB55_000052"
     CDS             50568..50969
                     /locus_tag="POB55_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDI6499052.1"
                     /translation="MKTILKTITYLALTIIGAYAALFILKTIDSHGITDQFNPLVKED
                     VSYVKTTEVSTRMDDQLRSYSQSAFNKEGKETQLMYTATFDVKPHRYLKITHKGHHVE
                     TFEEVEKGQVPKKSIRQIESIIACLYYMVQL"
     gene            51117..51689
                     /locus_tag="POB55_000053"
     CDS             51117..51689
                     /locus_tag="POB55_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDI6499053.1"
                     /translation="MKLKSLAVLSMSAVVLTACGNDTPKDETKSTESNTNQDTNTTKD
                     VIALKDVKTSPEDAVKKAEETYKGQKLKGISFENSNGEWAYKVTQQKSGEESEVLVAD
                     KNKKVINKKTEKEDTVNENDNFKYSDAIDYKKAIKEGQKEFDGDIKEWSLEKDDGKLV
                     YNIDLKKGNKKQEVTVDAKNGKVLKSEQDH"
     gene            complement(51827..52090)
                     /locus_tag="POB55_000054"
     CDS             complement(51827..52090)
                     /locus_tag="POB55_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI6499054.1"
                     /translation="MNMHILYNLRTKHNLEIDELAQQLNEKYGTKYEAHQIWEWENHH
                     HEPKFKDAMHLADFFDAPYEMFLESKVKEYQKHLEEVDIRMDK"
     gene            52384..52635
                     /locus_tag="POB55_000055"
     CDS             52384..52635
                     /locus_tag="POB55_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDI6499055.1"
                     /translation="MFGFIGMLIIGGLIGWAAGAIMGKDIPGGILGNIIAGIIGSWVG
                     GKLFGQWGPELGSIYILPALIGSIILIAIVTLILRAMRK"
     gene            complement(52674..52883)
                     /locus_tag="POB55_000056"
     CDS             complement(52674..52883)
                     /locus_tag="POB55_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499056.1"
                     /translation="MTTQSFIFMNIILLDGATQRHPFFICQILAFLFPKFPHINLLLF
                     NPILSTFSNIIIHIDHFHANKKDDN"
BASE COUNT        18093 a         7962 c        10077 g        16821 t
ORIGIN      
        1 taatcaaaaa ataatatatg tcatgttata ttgaaggtgc agttgttttt cattctcaag
       61 agggggtcaa aaaaatactt ttgaggtgat tatatgttaa gaggacaaga agaaagaaag
      121 tatagtatta gaaagtattc aataggcgtg gtgtcagtgt tagcggctac aatgtttgtt
      181 gtgtcatcac atgaagcaca agcctcggaa aaaacaccaa ctaatgcagc ggtacaaaaa
      241 gaaacactaa atcaaccggg agaacaaggg aatgcgataa cgtcacatca aatgcagtca
      301 ggaaagcaat tagacgatat gcataaagag aatggtaaaa gtggaacagt gacagaaggt
      361 aaagatacgc ttcaattatc gaagtatcaa tcaacacaaa atagtaaaac aatcagaacg
      421 caaaatgata atcaagtaaa gcaagattct gaacgacaag gttctaaaca gtcacaccaa
      481 aataatgcga ctaataatac tgaacgtcaa aatgatcagg ttcaaaatac ccatcatgct
      541 gaacgtaatg gatcacaatc gacaacgtca caatcgaatg atgttgataa atcacaacca
      601 tccattccgg cacaaaaggt attacccaat catgataaag cagcaccaac ttcaactaca
      661 cccccgtcta atgataaaac tgcacctaaa tcaacaaaag cacaagatgc aaccacggac
      721 aaacatccaa atcaacaaga tacacatcaa cccgcgcatc aaatcataga tgcaaagcaa
      781 gatgatactg ttcgccaaag tgaacagaaa ccacaagttg gcgatttaag taaacatatc
      841 gatggtcaaa attccccaga gaaaccgaca gataaaaata ctgataataa acaactaatc
      901 aaagatgcgc ttcaagcgcc taaaacacgt tcgactacaa atgcagcagc agatgctaaa
      961 aaggttcgac cacttaaagc gaatcaagta caaccactta acaaatatcc agttgttttt
     1021 gtacatggat ttttaggatt agtaggcgat aatgcacctg ctttatatcc aaattattgg
     1081 ggtggaaata aatttaaagt tatcgaagaa ttgagaaagc aaggctataa tgtacatcaa
     1141 gcaagtgtaa gtgcatttgg tagtaactat gatcgcgctg tagaacttta ttattacatt
     1201 aaaggtggtc gcgtagatta tggcgcagca catgcagcta aatacggaca tgagcgctat
     1261 ggtaagactt ataaaggaat catgcctaat tgggaacctg gtaaaaaggt acatcttgta
     1321 gggcatagta tgggtggtca aacaattcgt ttaatggaag agtttttaag aaatggtaac
     1381 aaagaagaaa ttgcctatca taaagcgcat ggtggagaaa tatcaccatt attcactggt
     1441 ggtcataaca atatggttgc atcaatcaca acattagcaa caccacataa tggttcacaa
     1501 gcagctgata agtttggaaa tacagaagct gttagaaaaa tcatgttcgc tttaaatcga
     1561 tttatgggta acaagtattc gaatatcgat ttaggattaa cgcaatgggg ctttaaacaa
     1621 ttaccaaatg agagttacat tgactatata aaacgcgtta gtaaaagcaa aatttggaca
     1681 tcagacgaca atgctgccta tgatttaacg ttagatggct ctgcaaaatt gaacaacatg
     1741 acaagtatga atcctaatat tacgtatacg acttatacag gtgtatcatc tcatactggt
     1801 ccattaggtt atgaaaatcc tgatttaggt acatttttct taatggatac aacgagtaga
     1861 attattggtc atgatgcaag agaagaatgg cgtaaaaatg atggtgtcgt accagtgatt
     1921 tcgtcattac atccgtccaa tcaaccattt gttaatgtta cgaatgatga acctgccaca
     1981 cgcagaggta tctggcaagt taaaccaatc atacaaggat gggatcatgt cgattttatc
     2041 ggtgtggact tcctggattt caaacgtaaa ggtgcagaac ttgccaactt ctatacaggt
     2101 attataaatg acttgttgcg tgttgaagcg actgaaagta aaggaacaca attgaaagca
     2161 agttaaattc atcttctgaa tttaatatgc tatgtaaatc gtgctgttat catggcacat
     2221 cagatataag tagcatcaca gtgttgaatt taaaaatagt aaagtgaaat aaagcgcctg
     2281 tctcattagc gaaaactaaa gggacaggcg tatctgttta tgagcttaat aaattgtatg
     2341 aataatatgg ttgatcgaat aactgtttat catgatgata aattgagttt tttaaaataa
     2401 tgatatatta catcattgtt atagcgttta agaaatcaac aactttacga taaatagtga
     2461 ttgcttcgtc attaggtcta cgatcaaaat catgctcgtt tttattcacg cgttcaaatg
     2521 ttgaatgtgg aacatgattc atgatatgtt cgctttcctc aacgggaaca tcataatcgc
     2581 cattacaatg cgcaatgaaa acaggtggaa gtgttttaag ttcatctggt gcaatattat
     2641 attttgaatc agtataatca gcaatgttaa tcatatttat ccatttacct gtgccacgtg
     2701 cataaacgta gattaaaaaa cgttgtgcga tttgatcttg aacaaccggt gttggtgaag
     2761 tgagttgtgc aatcattgtt tcgtttacgc tttgagctat ttttgcgtaa taactattag
     2821 ttgttttaaa aggttcagtg ttgatgcgac tataaccata aaaatcaata acaccatcaa
     2881 tatctctgtc tcgtgcaatt aatagactta aatatgcacc tgatgatctg ccaaaggtaa
     2941 aaatagggca attagaatat tgtgattgaa tcgcatcgaa tgatgcgtag acatcctcaa
     3001 taatgcaatc gagacttact tctggtaata aacgataact tagttgaatt aaatcgtaat
     3061 gttccgtaag gatatcgata tactgtgggg ataaatcgtt agctttaccg aacattaatc
     3121 caccaccgtg gatgtagaca atagcgcctt ttgttggttg attttttgct ttaataattg
     3181 tgtaaggtaa tgcaaatgca tctttagtaa ttactttatc tttaatttca gtcacgattt
     3241 aataggctcc ttatttttga tattgatgtc attataacac tgtcttaaat ttccatgaaa
     3301 aatagtctta agacgatgag tcatgataat tctgttccaa ttgacgtaaa gcgtcacggg
     3361 tatgcttctt tagaccttcc ccataatcca tcattttaac aatatcttta aaagcagcat
     3421 gtggaatggc taaatcttct aaatctgcca tagaaaattc aagattgata tcatgtggtc
     3481 gctgttcagc aagtttatgc acaaagtcag gttctgtgac aaaaggcgaa gacatgccga
     3541 ccatatctgc atgttgtaaa gcatctaaag cagactctgg agaattaatc ccgccacttg
     3601 caattaaagg gatacgacct gctaaatgtt catagacaat ttggttaact ggtcgaccga
     3661 aatgatcacc tggtgtacga gacgtatttt gataaatatg tcgaccccag ctagcgattg
     3721 ctaagtattg gatgtttgaa acgtccatga cccaatcgat taattggttg aactcgtcaa
     3781 tggtatatcc taaatcactg cctctggttt cttctggcgt tgctcgaaat cctaaaataa
     3841 aattgtcagg tgcttcttta tcaatcactt cttgtaccgc acgcataact tctaaacata
     3901 atcttgcacg attttttaat gagtcggcac cgtaatggtc tgtacgttta ttcgaaaaag
     3961 ttgagaaaaa tgtttgaatc agcaaacgtt gtgcaatcga aatttccaca ccatcaaaac
     4021 ctgctttaat cgcgcgtaat gtagcatcgc gatactgctg aatgatgcta ttgattttct
     4081 catgagacat ggcgataaca tcgtgttcaa tcggtgaatg caatgtcata gggcttggtc
     4141 catacacctt tccaaaattt aaaatggctt gatttgaaaa acgaccagca tgcgctagct
     4201 ggataatagc gaggctacca tgttgtttca tcgtagatgc catgttagtt aatccaggga
     4261 tacaagcatc atgatcaata ttaaagccat attcaaacaa ttgaccataa ggttcaatgt
     4321 aagcagcgcc ggtgacttgc attccagctg aattagagcg acgtgcagca taagccaagt
     4381 cttcttttgt aatatagcct tcttttgttg atgtgtttac ggtcattggt gataatacaa
     4441 agcgattcga aattttgatg ccattaggta agtggattga ttgtaaaagt ggtttgtatc
     4501 ggtacatact atgattcctt ttctattcaa tattgttttc aaagtaccat ggaaagaatg
     4561 aataatcaat gatgaacagt cttgatagaa tagaattggt acatggaaag tatttttaaa
     4621 attaaactaa tgaatggcat ttgtaggtct gaaaatatga atatgaaaaa gaaaaataaa
     4681 ggcgaaaaga tataaaagtt aattgaaaaa cgttatcata tacgtgggta tatgaagagg
     4741 gaatggtatt aagaacgcta aaatgttatg tcggtttgac atgacaggat aagtttggag
     4801 atgacggatt ggttaaatta agcgtattag actatgcctt aatagatgaa ggtaaggatg
     4861 cacaaaaggc attgcaagat tcagtgacac ttgcaaaatt agcagatcga cttggcttta
     4921 agcgaatttg gtttacggaa catcataatg taccagcgtt tgcgtgtagt agtccagaac
     4981 ttttgatgat gcatacattg gcgcagacaa atcacatacg agttggctct ggtggtgtga
     5041 tgctgccgca ctatcgacct tataaaattg ctgagcattt tagaatgatg gcagcgttat
     5101 atccaaatcg tattgattta ggtattggta ataatccagg tactactatg gtaaagcaag
     5161 ctttagatgg aataaatcct acatatgata gttacgatga atcgatttcg ttattacgtg
     5221 attatcttac aataaaggat aaaccaagtg cgcatacgtt aggtgtccaa ccacacattg
     5281 atcattttcc agaaatgtgg ttattaagta gtagcgcaac atctgccaaa atagctgccg
     5341 aactaggtat agggctttct gttggaacat ttttgctacc agatataaat gcgatacatg
     5401 cagcgaagga taacattgat atttacaaaa aacatttcca agcatcaacg attaaaatgg
     5461 acgcaaaggt gatggcatct gtatttgtca ttgtagctga taacgaagcg gaagtagcag
     5521 cattacaaca tgccttagat gtttggttat taggtaaatt acaatttgca gaatttgaag
     5581 attttccttc agtagacaca gcacaaaagt ataagcttaa tgatcgagac aaagagatga
     5641 ttcaagcaca tcaagcacgc atcattgcag gtacacaaga aaaggttaaa gcacaattag
     5701 atgatttcat tgctacgttt gaagttgatg aggtgttagt agcaccgctt attccaggta
     5761 ttgaacagcg ttgtaaaaca ttaaaattac tcgcggaaat ttatttgtag cattttaaat
     5821 agaagagaaa ggatgaagat aagatgaaaa agttagccaa ttatttatgg gtagaaaaag
     5881 taggagattt gtatgtgttt agtatgacac ctgaattgca agatgatatt gggacagtag
     5941 gttatgttga attcgtaagt ccagatgaag ttaaagtgga tgatgaaatt gtgagtatcg
     6001 aagcatcgaa aacggtcatt gatgtgcaaa cgccattgtc aggaacgatt attgagcgaa
     6061 atacaaaagc ggaagaagaa ccgacaattt taaactctga aaaaccagaa gaaaattggt
     6121 tgttcaaatt ggatgatgtc gataaagaag cattcctagc attaccggag gcttaaatgg
     6181 aaacgttaaa atcaaataaa gcgagacttg aatatttaat caatgatatg catcgagaga
     6241 gaaatgacaa tgacgtattg gtaatgccat cttcatttga agatttgtgg gaattatatc
     6301 gaggcttagc aaatgtcaga ccggcattac ctgtaagtga tgaatattta gctgtacaag
     6361 atgctatgtt aagtgatttg aatcgtcaac atgttacgga tttgaaggat ttgaagccga
     6421 taaaaggtga caatatcttt gtttggcaag gtgatatcac gacgttaaaa atcgatgcta
     6481 ttgttaatgc tgcaaatagt cgttttctag gatgtatgca agctaatcat gactgcattg
     6541 ataatattat tcatacaaaa gcgggtgttc aagttcgact tgattgtgca gagatcattc
     6601 gacaacaagg gcgcaatgaa ggtgtaggta aagccaaaat aacacgtgga tataatttgc
     6661 cagcaaagta tataattcat acggttggtc cgcaaatacg tcgattgcct gtttcaaaga
     6721 tgaatcagga cttgttagct aaatgttatc ttagctgtct taaattggct gatcaacata
     6781 gtttaaatca tgtcgctttt tgctgtatat ctacaggtgt atttgctttt cctcaagatg
     6841 aagcagcaga aattgctgtt cgaacagtag aaagctatct caaagaaaca aattcaacat
     6901 tgaaagtcgt gttcaatgta tttacagata aggatttaca actgtataag gaggcattta
     6961 accgtgatgc agagtagtaa gtggaatgca atgtctctgt taatggatga caagacaaag
     7021 caggctgaag tattgcgtac tgcgattgat gaagcagatg cgatagtgat tggaattggt
     7081 gcaggcatgt ctgcatctga cggatttaca tatgtaggag agcgttttac ggaaaatttc
     7141 ccagatttta ttgaaaaata tcgcttcttt gatatgttgc aagcgagttt acatccttat
     7201 ggcagttggc aagagtattg ggcatttgag agtcgtttta ttacattaaa ctatttagat
     7261 caacctgtag gtcagtctta cctcgcttta aaatccttgg tggaaggtaa acagtaccac
     7321 attataacta cgaatgcaga taatgctttc gatgtagctg attatgatat gactcatgta
     7381 tttcatatac aaggggagta tatactgcaa cagtgtagtc agcattgtca tgctcaaacg
     7441 tatcgcaatg atgatttaat tcgtaaaatg gttgttgcgc aacaagatat gcttatacct
     7501 tgggagatga ttccaagatg tccaaaatgt gatgccccaa tggaagtgaa taaacgtaaa
     7561 gcggaagttg ggatggttga agatgctgaa tttcatgcgc aactacatcg ttataatgct
     7621 tttctagagc aacatcaaga tgataaagtg ttgtatttgg aaattggaat tggttatact
     7681 acaccacaat ttgtgaagca tccttttcag cgtatgacac gtaaaaatga aaatgcactt
     7741 tatatgacga tgaataaaaa ggcatatcgc attccgaatt caattcaaga acgtaccata
     7801 catttaactg aggatatctc aacattgatt acaacagcac tccggaacga tagcacaacg
     7861 caaaataaca acattggaga gacaaaagat gtacttaata gaaccgatta ggaatggaga
     7921 atatattact gatggtgcga ttgcactcgc tatgcaagtt tatgttaatc agcatatctt
     7981 tttagatgaa gatattttat tcccttatta ttgtgatcca aaagtggaaa ttggacgttt
     8041 tcaaaatact gctatagaag tgaatcaaga ttatatagat aaacacagta ttcaagtagt
     8101 tcgccgagat actggtggtg gcgctgtgta tgttgataaa ggtgccgtta atatgtgttg
     8161 tattttagaa caagacactt caatttatgg tgattttcaa cgattttatc agccagctat
     8221 aaaggcgttg catacattag gtgcaacaga tgtgatacaa agcggtagaa atgatttaac
     8281 attgaatggt aaaaaagtgt caggcgccgc aatgacatta atgaacaatc gtatttatgg
     8341 cggttattcg ctattacttg atgttaatta tgaagcaatg gataaagtgt taaagcctaa
     8401 tcgcaaaaag attgcatcga aagggattaa atctgtgcgc gcacgtgttg gtcatcttag
     8461 agaagcactg gatgaaaagt atcgtggtat aaccattgaa gaatttaaaa atttaatggt
     8521 gacacagatt ttgggaatcg atgacattaa agaggcaaaa cgatacgaat taacggatgc
     8581 agattgggaa gcgattgatg aattagttga taaaaagtat aaaaattggg attggaatta
     8641 tggcaagtca cccaaatatg aatataatcg aagtgaaaga ttatcatcag gtactgtaga
     8701 tattaccatt tctgttgaac aaaatcgtat tgcagattgt agaatttttg gtgatttctt
     8761 tggacaaggt gatataaaag atgtggaaga agcattacaa ggaacaaaaa tgacaagaga
     8821 agatttaacg catcagttaa agcaattaga catcgtttat tattttggta atgttacggt
     8881 tggatcattg gttgagatga ttttaagtta atattgttta ggcgttaatg tcgcaccctc
     8941 aatgaatatg tgtatttgct tttaaaatgg tgactatgta gttatgtaat ttgaacatac
     9001 gattgaaaat gaatttagtt aattagcaaa gacatcatat ttatagcgtt tgtgtttcta
     9061 aatgattggg tatttgaaaa tggttaatga tagtttttaa aggaataaat tcattttcat
     9121 ttatataagt aagattaaat actagctact aaagtctatt tatatacgtc aaaatataat
     9181 gactgctata atgagtaatc aatagacaca aagaggagat tatgtgatga ataataaagt
     9241 attagtaacc ggtggtacag ggtttgttgg catgcgaatt atttcacgat tattagaaca
     9301 aggttatgag gtacaaacaa cgatacgtga tttaagtaaa gctgataaag taattaaaac
     9361 aatgcaagac aatggcattt ccacagagcg attaatgttt gtcgaagcgg atttatcaca
     9421 agatgaacat tgggatgaag caatgaaaga ttgtaaatat gtcttgagtg tagcatctcc
     9481 ggtgtttttc ggtaaaacag acgatgcaga agtgatggcg aagcctgcca ttgaaggcat
     9541 acaacgtatt ttaagagctg cagaacatgc tggcgtgaag cgtgtggtga tgactgcaaa
     9601 ctttggtgca gttggtttta gcaataaaga taaaaattca atcacaaatg aaagtcattg
     9661 gacaaatgaa gatgaaccag gcttatcagt atatgaaaaa tcaaaattgt tagctgaaaa
     9721 ggcagcgtgg gattttgttg agaatgaaaa tacaacagta gaatttgcca caatcaatcc
     9781 agttgcaatt tttgggccat cattagatgc acacgtttca ggaagctttc atttattaga
     9841 aaatttattg aatggttcaa tgaaacgtgt accgcaaatt ccattgaatg ttgttgatgt
     9901 gagagacgta gctgaactac acattttggc aatgacaaat gaacaggcta atggcaagcg
     9961 atttattgct acagctgatg gacaaattaa tttgttggaa attgcaaaat taattaaaga
    10021 aaagagacct gaaatagctc aaaaagtttc tactaaaaaa ttaccagact ttgttttgag
    10081 tctaggtgct aaatttaatc atcaagctaa agaaggtaaa cttttattag atatgaatcg
    10141 aaatgtaagt aacgaacgtg caaaaatact tcttggttgg gaaccgattg cgacacaaaa
    10201 agaagcaatt ttagcagctg tcgatagtat ggctaagtat catttaatat aatacaaaac
    10261 accgtccata agtaagtggc tttcctcttg ataaaagagg cattcactca atatgtgacg
    10321 gtgttttata tatttacgtt aacactattg ctgtggcttt tgacggcctt ttaataaaat
    10381 tgctgtcgca cctacgataa taataaatag aatcgcacca aataatccca tatattttac
    10441 tgcgttaccg aacacgatac cgacagttaa aaagtctgta tctgagaatg ttgttgcagc
    10501 accacctaat tcgcctaaaa atggcaagaa taataatggt aaaaacgtta ttaggatacc
    10561 atttagagcg gcgccagcaa cagcaccttt aataccgcct cttgcattac cgaatacagc
    10621 agccgttgca cctaagaaga agtgtgcaac tacgccaggt aaaatgacga cgccaccaaa
    10681 taagaataag ataaacatac cgatgacacc tgtaataaag ctgacaaaga atccaattaa
    10741 tactgcattt tgtgcataag ggaacacaat agggcagtct aatgcaggtt tagaatttgg
    10801 tacaagcttt tcagaaattc ctttaaatgc tgggacgatt tcagctaaga ttaagcgaac
    10861 gcccgttaaa ataataaata caccagcagc aaatgtcaca ccttgaatta atgaaaagac
    10921 aataaagttt tgaccattac taatagactc gtgtacataa ctaacgcccg caaataagca
    10981 tgcgatgaag taaagtaatg ccatcgtaat cgagatacta attgtacttt ctcgtaagaa
    11041 acttaagcct tttggaaatt taatctcttc cgttgattta gacttacctt tgaataattg
    11101 acctacagca cctgcggcaa agtaactgat tgagccaaaa tgacctaaag ctacttggtc
    11161 attccctgta atttttcgca tcgtaggttg gagtaatgca ggtaatactg ccatgattaa
    11221 tcctaatacg agtgcgccga taacaatcgt tagccagcct ttaatatgac tgactgttaa
    11281 aatgattgct aaaaacgcag ccatgtaaaa tgtatgatga cctgttaaaa agatatattt
    11341 taaattagtg aagcgggcaa ttaaaatatt aacaatcatg ccacagacca tgatgagtgc
    11401 agctgttgtt ccaaaatctt ttaaggctag tgagacgata gcttcgttgt taggtacgat
    11461 accttgcaca ccaaatgcgt gttggaatat tttgccgaat ggttcaagag atcgaacgac
    11521 gacatcagca cctgcactta aaattaagaa gcctaatatc gttttaatgg ttcctgaagt
    11581 gatcgttgcg gcaggttttt tctgaacgat taaacctata aaggcaatca gtgcaacaag
    11641 aatggctggt tgacttaaaa tatcgactat aaaattaagg attgcttgca taggtcgtac
    11701 ctcctttaaa tcatgttaag ttgttgtaat ttttctgaga gcttttgttg taattcagct
    11761 ttatctaaaa tattatcaag aactaagaca tcccctagac gttcggcatt ttcagctaaa
    11821 tctctaccac aaataaacaa gtcagccatc tctggacttg ctgtcataat gtcactatgt
    11881 tcaacttcga tatcagatgg tgcattaagt tgcctaagtg cttcttgtgc gttcatttct
    11941 accataaaac tacttcctaa accgtggcca catactacta aaattttcat attaatcatg
    12001 ctcctttaaa atgtttttaa tgtcttgtgc atttgttgca gttaatagtt gctggactgt
    12061 ttggttatcg cccagtacgg ttgctaaatt ttgtaataca gataagtgtg aatgattgtc
    12121 gatggcactc aatacaaaaa tgagagatgc gtagtgatct tcatcacaaa atgccacatg
    12181 ttgattcaac tttaatagac ttaaaccaac ttgatgtacg tcattgttcg gtcttgcatg
    12241 tgcaattgca atttcaggtg cgataacgat ataaggtcca agttcattaa cgctatcaat
    12301 cattgcttga acatagcctt gttcaataat ttgttcttgt agtaatggct gagaagctat
    12361 agttatagct tcagtccaat catttacttg ttcttttaca atgatgcgtg ttgttgacaa
    12421 aatgtctaat gacacgttat taagcctcct ttgtcatagt taaagcaatg tgttgtttaa
    12481 ttttaaaaat attcccatct aagaaatctt gtcgatataa gtcgttgctt aagcattcgc
    12541 ttaactgtcc caatgccttt aaatgtgcat tggggtggtc cgttgctaat gtaattacaa
    12601 ggtgaacggg atcgttagct ttactaccaa agataatccc ttcagtgaaa tatgttagtg
    12661 cgaaacctac accattctgt acataatcag taccagcgtg aataagtgca atatgtggac
    12721 taatgaccat atatgacccg aattgttcaa attgttttaa aattgcagct gtataatttg
    12781 aatagacaat gccatcattg attaaaggtt gcacagccac tgcaattgcg gattcaattg
    12841 ataatggttg tttatttata atgatgcgat gttcaggcaa taaatctgcg agtgacttgc
    12901 catcagttgc cattttcatg actcgttgtt ctcttgagtc attgataatt tgattcaatt
    12961 tttgacgaga ttgttgattg ataaatggat cgacatgaat aactggtaca gctgatattt
    13021 cacaaggtac tgttgaaatg acataatcaa tgttatcttg caataatcga ctttcttcca
    13081 attgataaat ggaataggca tcccaaatgt gaaactcagg atacaggtga tttagttttg
    13141 attttaaaag ttgtgacgtg cctataccag aaccacatag taagacaacc ttaatcattg
    13201 attgtttatg tgttgcaaca cgctctatac ttgatgcgaa gtgaattgta atgtatgtta
    13261 attcatcttc gttgaagcga atagcagcat cttgttcaat tggactaata tgcttgctaa
    13321 cggcttcaat gatttgagga tagcgacgca taacttcttg cctcaaagga ttaggttgta
    13381 gcatatcgta tttaatacga tgtatagctg gtttgatatg tgtgatcaga ctggtatgta
    13441 acttgttgtc ttttgacata tcaatgccta attcttggct aacacaagtg atcaattcat
    13501 gtatattttg cgataaatca tggtattcaa aggtaattga agatgctgta tgttcagtca
    13561 ttttagagcc tagtaaatgt aacgtgataa agataatttc agactctgga aatgtgacat
    13621 tacaactgcg ttctaagttt tctatcattt ttgaagcaat agcatactga ttagtatgtc
    13681 gccatttatc aatttcattg ataggtatat cgaacgaaaa attttcattt aaacgctgaa
    13741 tggcaatgag tatatgatag attaagccat cgatagccga ctgaactaaa tgataatttt
    13801 cactatttaa tgtcttaata atggcacggc gaaccaatgc gattgattct gaattaaaga
    13861 tatccgcctc tataaaaggt gcagcttgtt tcatatattg atgtataaag tgtgcatacg
    13921 ctttacgata atgatcttcc tcaccaataa tattgaatcc tttattgtgg acataattta
    13981 actttaaatg gtattgatct agttgggctt gaatcatttt aatatcatct gcaattgtcc
    14041 gacgcgaaac attaacatct tgcgcaagtt gctttgttga aacaggatcg gttgtttcga
    14101 ataactttaa agcgatatgt gtgagtcgtt catcttttga aaaatgaatt tgatttgtta
    14161 gattgtgctc taattcattt aatagtgtcg cgtgagctgt tgttactttg atgcctgcag
    14221 ctttattacg gctgacttgg taatgataag tttcagcata ttgctcaata tatgctatat
    14281 catattgaat ggtacgaggt gatacaccaa gttgattagc aatggtattg attggaataa
    14341 acgtttgctc atgaattaaa agatacaaaa tttcgatttg tctataactt aacaacgtaa
    14401 tatcctccta tttgtaattg taagcgattt cttaaaaacg tagatatgca atctctttca
    14461 tattttaatc cgaaaaattg catatcaaaa tgtttatggc gcaagatttt ataggaactt
    14521 ttaaaataaa ttagatattc atgttgacaa tttaaaaatg tcgcagtata tttagttaga
    14581 catctaacga aatggtggtg caataaatgg aattcactta ttcgtattta tttagaatga
    14641 ttagtcatga gatgaaacaa aaggctgatc aaaagttaga gcaatttgat attacaaatg
    14701 agcaaggtca tacgttaggt tatctttatg cacatcaaca agatggactg acacaaaatg
    14761 atattgctaa agcattacaa cgaacaggtc caactgtcag taatttatta aggaaccttg
    14821 aacgtaaaaa gctgatctat cgctatgtcg atgcacaaga tacgagaaga aagaatatag
    14881 ggctgactac ctctgggatt aaactcgtag aagcattcac ttcgatattt gatgaaatgg
    14941 aacaaacact cgtatcgcag ttatctgaag aagaaaatga acaaatgaaa gcaaacttaa
    15001 ctaaaatgtt atctagttta caataaatga taagtgtgac tggtagaaat cagtcacttt
    15061 gtctttaata ttatagttag atatctaatt gttagtaagc taattattgg aaaagacaag
    15121 gagtattgaa caatgaaaga cgaacaatta tattattttg agaaatcgcc agtatttaaa
    15181 gcgatgatgc atttctcatt gccaatgatg atagggactt tattaagcgt tatttatggc
    15241 atattaaata tttactttat aggattttta gaagatagcc acatgatttc tgctatctct
    15301 ctaacactgc cagtatttgc tatcttaatg gggttaggta atttatttgg cgttggtgca
    15361 ggaacttata tttcacgttt attaggtgcg aaagactata gtaagagtaa atttgtaagt
    15421 agtttctcta tttatggtgg tattgcacta ggacttatcg tgattttagt tactttacca
    15481 ttcagtgatc aaatcgcagc aattttaggg gcgagaggtg aaacgttagc tttaacaagt
    15541 aattatttga aagtaatgtt tttaagtgca ccttttgtaa ttttgttctt catattagaa
    15601 caatttgcac gtgcaattgg agcaccaatg atttctatga ttggtatgtt agctagtgta
    15661 ggcttaaata ttattttaga tccaatttta atttttggtt ttgatttaaa cgttgttggt
    15721 gcagctttgg gtactgcaat cagtaatgtt gctgctgctc tgttctttat cgtttatttt
    15781 atgaaaaata gtgacgttgt gtcagttaat attaaacttg cgaaacctaa taaagaaatg
    15841 ctttctgaaa tctttaaaat cggtattcct gcatttttaa tgagtatctt aatgggattc
    15901 acaggattag ttttaaattt atttttagca cattatggaa acttcgcgat tgcaagttat
    15961 ggtatctcat ttagacttgt gcaatttcca gaacttatta tcatgggatt atgtgaaggt
    16021 gttgtaccac taattgcata taactttatg gcaaataaag gtcgtatgaa agacgttatc
    16081 aaagcagtta tcatgtctat cggtgttatc tttgttgtat gtatgattgc tgtatttaca
    16141 attggacatc atatggtcgg actatttact actgatcaag acatcgttga gatggcgaca
    16201 tttattttga aagtaacaat gacatcattg ttattaaatg gtataggttt cttgtttact
    16261 ggtatgcttc aagcgactgg gcaaggtcgt ggtgctacaa ttatggccat tttacaaggt
    16321 gcgattatca ttcctgtatt atttattatg aatgctttgt tcggcctcac aggtgtcata
    16381 tggtcattat taattgctga gtcactttgt gctttggcag caatgttaat cgtctattta
    16441 ttacgtgatc gtttgacagt tgatacatct gaattaatag aaggttaaat atttcgtaca
    16501 cttctggctg agtatatttc ggtcggaagt gtatttttcg aataaaaaat aaatatatga
    16561 tacgattatg aaaaaaataa agtgagaatg acatatgtat aaatctaaaa tactgttgaa
    16621 atatattttt agtgaagaac cagaagttaa agatttaact gaagaaaaat ataatcaaga
    16681 ttacgaagca ttaacattta gctttaaaga ggaaacatat caaagtaggt tagctaagaa
    16741 aacaccgact aaagcagggt attttgttac atgttggaca aaagacgaga acaattgtaa
    16801 tcaaccatac tcaaaagagg catttgcaga ttacttaatg attatagtta ttgatgaaga
    16861 attaagcggt tattttttat ttcctaggga attattggta gaaaaaggta tcttaactac
    16921 atttgaacat aaaggtaaga tggcttttag agtttatcct aagtggtgta atcaattgaa
    16981 taaaacagca gggcaaacac aaaagtggca atgtaaatat ttttttgaat actaataaaa
    17041 agtcatatgg ttgaacttat aagagatgaa tatatcatct ttcgataaga taaccatatg
    17101 actttatttt ttatcattta atgatgaacg gtttcttgtc ctactttatt ccaagtgagg
    17161 ataaagctca acattgcaaa cacactgatt gctgttaata aaataaaacc gacatcccat
    17221 ccgaatttat caactacagc acctaagacg atgttggcca ttacagcacc aaacagataa
    17281 ccaaataatc ctgttaatcc agctgctgtg ccagctgctt ttttaggtac ataatctaat
    17341 gcttgtaaac caattaacat aactggtcca tatattaaga aaccaatggc aattaatgag
    17401 acattgtcta accaagcatt gcctggagga tttaaccaat aaattaatac aaatactgtg
    17461 acacctaaca taaagaagaa acctgcaggt cctcgacgac ctttgaataa tttatcagaa
    17521 atgtaaccac ataataatgt accaggaatt ccagcccatt cgtataagaa gtatgcccaa
    17581 cctgatgctt ttaagtcgaa atgcttttct tcacttaagt agactggcgc ccaatcaagt
    17641 acaccataac gcacgaaata aacaaatata tttgcaaagg caattgccca tacccattta
    17701 ttgtttagta catatttaaa taaaatttct tttgtagtta attctgtttc taatgttttc
    17761 ttatcgcttg tagcaaagtc gtttttataa atttcgattg gaggtaaacc ttgagattga
    17821 ggtgtgtctc taatcaatac gtatgaaatt gcggcaatga taagtgctaa gagtgcaggg
    17881 taaatgaata caccttcgaa cccttttaaa taaccaaagt tgataaatgc tgttgttgta
    17941 ataccccaag cagcaatagg tgccataata cctccaccaa cattatgcgc aacgttccaa
    18001 agggcagtct tacttccgcg ttcacttaca ctaaaccagt gaacgagaac acggcctgaa
    18061 ggtggccagc ccataccttg aaaccatcca tttaagaata ataggacaaa cataataccg
    18121 atacctgatg taaagaacgg tacaaatccc attaacaaat tgacgatagc agtgagtgct
    18181 aatccaagaa ctaagaatat ccgagcattg ctccgatcac ttacagtacc cataaagaac
    18241 ttactaaatc catatgcgat ggaaacagca gaaagtgcaa aacctagttc cgcttttgta
    18301 aaaccttgct cttgcaatgc tggcatcgct aatgaaaagt ttttacgtaa taaatagtac
    18361 ccagcgtaac cgatgaaaat accaagaaat acttggagac gtaatcgttt ataggtatca
    18421 tctatctgat tttctggcaa aggcttaata tgctttgcag gtttaagaaa attcataaaa
    18481 tcctccttaa tatgtattta tatgcatttt gtgcgataaa cttatattag acatgtatga
    18541 aagcgttgtc aatatacttt tgtgaaatct tgcatattta gttattgaag tattttataa
    18601 acatttagaa aaaataaatt gctacacata aatttataat tatgcaatta ttatgtcgtt
    18661 atacatacta gctgtgtatc tacgaatgag tacaaacata tttttatttg cagaaaaggg
    18721 gtaaatggca tataactatc ttttttatgt aagttggtat aaaatttata ctaataggag
    18781 ggatagtatg aatatagtag ggcatcatca catatccatg tatacaaaag atgcaaaacg
    18841 taataaggat ttttacacaa atgtccttgg attacgatta gttgaaaagt cggttaatca
    18901 agacaatcct tcaatgtatc atttgtttta tggggacgaa gtaggtacag ccggaacaat
    18961 tttaagcttt tttgaaattc ccaatgcggg tcataagcag ccaggtactg aaacgattta
    19021 tcgattttct ttattagtac caaatcaagc ggcacttcat tattttgaaa aacgccttga
    19081 taataatggt attacgtctg aacgtttgta ctatcttgga caagaaggtg ttgtctttaa
    19141 agatgaagac gacttagaaa tcatattgct tgttaatgat agttttgaag taccacatca
    19201 atggcaacat aacgtttata gtgaaatacc tcaagcatat caaattttag gaatagggcc
    19261 agtcgaatta agagttagaa atgcagcgcg tacggtagaa tttttggaaa atgtcttagg
    19321 ttatcgcaaa agagataata aatcattcga tgtgctgaca ttagcaccac aaggtttata
    19381 ttcggatttt gtagttattg agcaacaggg acaacgtgaa agacctggac gaggttatat
    19441 ccatcatatt gcaattaata caccacaaat gagtgactta gatgcaattt acaagaaatt
    19501 acaacaacaa acacaaagta attcaggtat aattgatcgc tatttcttta aatcattata
    19561 ctatcgccat aattcaatta tgtatgaatt tgcgactgaa gcgcctggat ttactattga
    19621 tacacctctt gaacaattag gaagtcaatt gaacttgcct gactttttag aagcagaacg
    19681 tgaacaaatt gaaagtaagt tacacgaaat ataaaggaga atgtttaatg gcaaaattag
    19741 aaatgaataa aaatacacct cttgagtttg gtttgtattc cttaggtgat catttattga
    19801 atccattgaa aggtgaaaaa gttagttatg agcaacgtat taatgaaatt attgaagcaa
    19861 gtaaattagc agatgaagca ggtattgatg tttttgcagt tggtgaaagt catcaggagc
    19921 attttacaac acaggcacct acggttgtgt taggtgcaat tgcccaagcg acaaagcata
    19981 tcaaagtttc aagttcttca acgattatta gtgcaacaga tcctgtaaga gtatttgaag
    20041 acttcgcgac attagatttg atttctcatg gtagagccga aattgtagct ggcagagcat
    20101 caagaacagg tatttttgac ttgtttggct atgatttaaa agactatgat gaattgtttg
    20161 aagaaaaatt aggtttactt ttagagttaa ataaaactga gcgtattact tggtctggaa
    20221 aatatcgtcc agaacttaga aatatgaaaa tattcccaag accaatcgat aatacattgc
    20281 caatatggcg tgctgttggt ggtccacctg caagtgctat taaagcggga aaacaaggtg
    20341 tgccaatgat gattacaacc cttggtggcc cagcaatgaa ctttaaaggt tctatagatg
    20401 cttatcgtca agcagcaact gaagcaggtt tcgatgcttc gcctaagtct ttaccagtaa
    20461 gtatagcgag tctgttttat acagctgaaa caactcagga tgctatgaga gaattttatc
    20521 cacatttgaa tacagggatg tcatttattc gtggcgttgg ttatccgaaa cagcaatttg
    20581 ctaattcacc agattataga gaagcgctaa tggttggaag cccgcaacaa attattgaaa
    20641 agatattgta tcaacacgag ttgtatggtc atcaacgttt tatggcacag cttgattttg
    20701 gcggtgtgcc atttgaaaat gttatgaaga atatagagtt aattggcaac gacattatac
    20761 cggcgattaa aaagcattta tcaaaatagg aggggcgtca tcatgaatat tgtattattg
    20821 tcaggttcca cagtaggttc taaaacgaga attgctatgg atgatttaaa aaatgaacta
    20881 gaagtcatca atgagggaca tcaaatagag ttgatggatt tacgagaact tgaattagaa
    20941 tttagcgttg gaaagaatta tctagatact acaggagatg tatataaatt aacgacgtcg
    21001 ttaatgcagg ctgatgtgat ttttattggt tttccaattt ttcaagcttc catccctggt
    21061 gctttgaaaa atgtgtttga tctacttcca gtcaatgcgt ttcgtgacaa ggtaatagga
    21121 cttgtagcga cagcaggttc tagtaaacat tatttaattc ctgaaatgca tttaaaacca
    21181 atattgagtt acatgaaagc acatacgatg caaacgtatg tatttattga agagaaagat
    21241 ttttcaaatc aacaaattgt caatgatgat gttgtatttc ggttaaaagc gttggcacaa
    21301 tccacaatgc gaactgccaa agtacaacaa caagtgtttg aagaagaaaa caaccaatac
    21361 gacttttaaa gtataaaaat aagacgctcg gcacactaaa tttgcaagtg tttgagcgtc
    21421 ttttcatatt aactatatag ccaatgaacg acgataaagg caagtgatga caagcatatt
    21481 gaggtaataa tgattgtcat aagcggttta agtgcgcgat ttttaagatc tttaaatgca
    21541 acatttaacc ctaaagcaac catggccatt aataagcaaa ttgttgatac agtatttaaa
    21601 atatttagca atgctgacgg aatagttaca tatgtattca ctaaggccat aatgacaaat
    21661 ccaattaaaa agtatggaat gcttattcga ccattgctag atgattctga tgaacggaaa
    21721 cgcataatta aaataagtac gatggttaat ggaatcagta agaatactct gccaagttta
    21781 ccaagaagtg caattttaag tgcatcacta ccaccaaagc caccagctag gacaacgtgt
    21841 gcaatttcat gaagactaac gccagaccaa gcgccataaa catttgtcgt cattgaaaag
    21901 atagcataga tagctgtata tataagtgaa aatatcgtac caatcaatgc gatgataccg
    21961 atactaatag ctgtatcctt ttcacgtgat ttgaatattg gagcgactgc agcaatagcg
    22021 gcagcaccac aaacgcctgt acctacacct agtaataatg cgatgttttt gtcaccatgc
    22081 aacagtttgt tgacaaagag catcattaca atactaaaaa taacgacacc tacatcgatg
    22141 gctaatagtc tactaccttg accgataata tcgaatatat tgagtttaag tccatatagg
    22201 atgattgcaa atcttaataa atatttagat gaaaacgtaa tacctgagct atattgttca
    22261 ggatatcctc taaagtgacg atatagaata gcgattaata tcgcgatagt taatgcgcca
    22321 accttatcta agattggcaa tttagcagct aaaaagctaa ataatgcgac tataaatgtt
    22381 aatgttagtc caatcataaa atgcttattt ttcaatgatg ccatgagcag tgcctccttt
    22441 aatagcattt tagcactgtt ttgtcgtatt tttaaatata aatttggaat ggttaataaa
    22501 gtagagatta aattaagttg tgtgatagga aacttggaca tcaatcaaag taataggcac
    22561 tacaacgctt attggcgggg ccccaacaaa gagaatttcg aaaagaaatt ctacaagcaa
    22621 tgcaagttgg ggaaggacaa caaatttaag atacaaggcg taatattaat atgttattat
    22681 tatgataatt tacaaaatta tatgaaaaat aaatgagggt gtgatggtat gtttggaatg
    22741 aaagtgaatg aacaaataat attaaaaatt ttagaagccc atgacacaga agcgcttttc
    22801 aatttagtca atcgttcaag aaattcactt agggaatggt taccttgggt agatgcaact
    22861 gagcaaccat cagatacgcg tgcgtttatt aaaagaggac ttttgcaatt ttctgatggt
    22921 aatggatttc agtgtggcat ttggtatgaa ggaacgctag ttggtgtcat cggtttacat
    22981 gaaattaatc gcatgcacag aaaaacttca ttaggatatt atttagataa acaatttgag
    23041 ggtcatggga ttatgacaca agcagttgag gcattgataa agtattgttt cgaagagctt
    23101 gacttaaacc gaattgagat tagtgccgca gttaataatg aaaaaagccg agctattcct
    23161 gaaaggctgg gatttactag agaaggtatg ttacgtgaca atgaattact aaatggtatt
    23221 tattcatcga gttacatcta tagtttatta aaatcagaat acgaccaaaa atgacaaatt
    23281 agacttacaa aagagtgatg acatttaaaa tggcatcgct ctttcattta attttttaaa
    23341 ataaaaggtt gttgacagta ttattttata acaatataat gattttgata attattatca
    23401 actagataat gttcatggga ggatgcctta aaatcatcat tttaagagcc acgtattatc
    23461 ttggcattta gggagtgtga gatagtacga atgagggcat gtctatgggg ataatagaat
    23521 ttctataatg aggtgtcaaa atgaaaaagt taacaacgct attattagca tcaacgttat
    23581 taattgctgc atgtgggaac gacgatagta agaaggatga ttcaaagaca tcgaaaaaag
    23641 atgatggtgt taaagcagaa ttaaaacaag caacaaaagc atatgataaa tatactgatg
    23701 aacagttaaa tgaattttta aaaggtacag aaaaatttgt taaagcgatt gaaaataatg
    23761 atatggccca agcaaaagcg ttatatccaa aagttcgtat gtattatgaa cgctctgaac
    23821 cagttgcaga agcatttgga gatttagatc ctaaaattga tgcacgtctt gcagatatga
    23881 aagaagagaa aaaagaaaaa gaatggtcag gatatcataa gattgaaaaa gcattatacg
    23941 aagataagaa aattgatgat gtgactaaaa aagatgcaca acaattattg aaagatgcaa
    24001 aagaattgca tgccaaagct gatacattag atatcacacc aaaattaatg ttacaaggtt
    24061 ctgttgacct attaaatgaa gttgcaactt ctaaaatcac aggtgaagaa gaaatttatt
    24121 cacatacaga tttatatgat tttaaagcga acattgaagg tgcacaaaaa atttatgact
    24181 tatttaaacc tattttagag aaaaaagata aaaaattaag tgatgatatt caaatgaact
    24241 tcgataaagt gaatcaatta ttggataaat ataaagataa caacggcggt tatgagtcat
    24301 ttgaaaaagt atctaagaaa gaccgtaaag catttgcgga tgctgttaat gcattaggag
    24361 agccactaag taaaatggct gtgattactg aatgacaaat tatgaacaag ttaacgatag
    24421 tacgcaattt tcaagacgta catttttgaa aatgttaggt attggcggtg ccggtgttgc
    24481 aattggcgca agtggtgttg gtagcatgtg gtctttcaaa tcaatgttca atacaccaga
    24541 agatccggaa aaagatgcgt atgaatttta tggtaaagtg caaccaggca ttaccacacc
    24601 cacgcaaaaa acatgcaatt tcgttgcgtt agatttgaag tcaaaagata gagatgcaat
    24661 taaggcaatg tttaaaaagt ggacggttat ggctgatcgt atgatggatg gtgataaagt
    24721 tggcaagccg agtaacaatc ctttaatgcc accagtagat accggtgaat cgataggatt
    24781 aggtgcaagc aagttaacga ttacctttgg gattagtaag tctttgatga agaaaattgg
    24841 gttatctagt aaaattcccg atgcctttaa agatttaccg cattttccga atgatcagtt
    24901 aatagacgat tacagcgatg gtgatattat gattcaagca tgctcaaatg attcgcaagt
    24961 atcctttcat gcggttcata atttagttcg tccatttcga gatattgtta aggtacgttg
    25021 ggcgcaatct ggttttatct ctgctaaagg taaggaaaca tctagaaatt taatggcatt
    25081 taaagatgga acaattaatc ctagaaagag taatcaactt aaagattatg tgtttattga
    25141 tgacggatgg gcgaaacatg gaacttattg tgttgtcaga cgtattcaaa tacacattga
    25201 aacgtgggat cgtactgcac tggaagaaca agaggctaca tttggtcgga aacgacatag
    25261 tggtgcgccg ttaacaggtg ggaaagagtt tgatgaaatt gacttaaaag cgaaagatag
    25321 tcatggcgag tatattattg ataaagatgc ccatacgaga ctagcgaaag aagcaaatac
    25381 gtcaatttta cgtagagcct ttaattatgt tgatggtacg gatgaccgca caggtaactt
    25441 cgaaacaggc ttgttgttca ttgcttttca aaaagcgaca aaacaattta tcgatataca
    25501 aaataattta ggtagtaatg ataaattaaa tgaatatatt acacatagag gttctgcttc
    25561 atttttagta ttaccaggtg ttagtaaggg aggatacctt ggtgaaacat tatttgatta
    25621 aatttgtagc aatgctaata actgctgcta tggtgtgtag ctttgggtta ctgaaaagtc
    25681 aggcagcaaa acaacaaagt attagtgatg tatatagtgt gataacggat gcgaaatctg
    25741 cactttctaa taattcgata tcgaatgaca ataagcagaa agcaattgag caagtggtaa
    25801 gtgcagttaa gaaattatcg cttgaagata atagcgaagg taatgctgtc aaatcagatg
    25861 tgagaaagct tgaagatgca aaagcgaatg ataatcaaaa agatacactt tcgcaattaa
    25921 cgaagtcatt aattgcttat gaagagaaat tggctagtaa agatgcgggt tctaaaatta
    25981 aactattgca acagcaagtc gatgccaaag atgcagtaat gacaaaagcg attaaagata
    26041 aaaataaagc agagctagaa tcgctgaaca atagtttgaa tcagatttgg acaagtaatg
    26101 aaacggtaat tcgcaattat gacgcaaatc aatatggaca aattgaagtc gcattactgc
    26161 aacttagaat cgcaattcat aagtcgccat tagatacagc gaaagtgtca catgcttgga
    26221 caacttttaa atcaaatatt gatcatgtcg ataagaaaag tgatacgtct gcaaaagatc
    26281 aataccatgt atcacaatta aatgatgagt tagagaaggc gattaaagct atcgacgaca
    26341 atcaattgtc ggatgctgat gctgcgctta cacattttat agaaatttgg ccgtatgttg
    26401 aaggtcaaat tcaaactaaa gacggtgctt tgtatacgaa aattgaagat aaaataccat
    26461 attatcaaag tgtattagac gaacataata aagcacatgt gaaagatggt ttagtagatt
    26521 taaataacca aattaaagag gttgttggcc atagttatag ctttgttgat gtgatgatta
    26581 tctttttacg tgaaggtcta gaagtgttat taattgtaat gacattgact actatgacgc
    26641 gtaatgtcaa agataagaag ggtactgcaa gtgtgattgg tggtgcaatt gccggacttg
    26701 tactgagtat tatcttagca attacgtttg tagaaacttt aggtaatagt ggcattcttc
    26761 gtgaaagtat ggaagcggga ttaggtattg ttgcggtcat attaatgttt atcgttggtg
    26821 tttggatgca caaacgttca aatgcgaaac gctggaatga tatgattaaa aatatgtatg
    26881 ccaatgcgat tagtaatggt aatttggtat tgttagcgac gattggttta atatctgtgt
    26941 tgcgtgaagg tgttgaggtt atcattttct atatggggat gataggtgag ctagcgacaa
    27001 aagattttat tattggtatt gctttagcta tcgttatatt aattatcttt gcgttattat
    27061 ttagatttat cgtcagattg atacctatat tctatatatt tagagtgttg tcgattttta
    27121 tttttattat gggattcaaa atgcttggcg taagtattca aaagttacaa ttattaggtg
    27181 ctatgccgag acatgtcatt gaaggattcc caacgattaa ctggttagga ttctatccaa
    27241 cttatgaacc attgatagca caagctgcct atattatggt agttgctatc ttaatcttta
    27301 aatttaaaaa ataaaaaaca ggccgagtgc ctgttttttt gttgcgatgt tggaaatatt
    27361 cggtattgca gtataacgat aatcacagca ttaattctta taagattaat gtgttggcgg
    27421 tttgcctcgg tatgtaaatt taacgatgaa cgtactgaac tcaaagagca atatgagtgg
    27481 caatgtgagt aatatattta atgttaaatc gggtggtgca atgatacttg ctaatacaaa
    27541 gcaagcgaaa taaatatatt ttcgataatg cttcaatgat gtggtatcta taagaccgaa
    27601 ttttgcaagt cctatgaata atattggtaa ttgaaataga atgccaaatg tgaataacca
    27661 acgtataagt tcaatcaaat atgctttaaa gccaatgaca ggcgaaatgt ttaaagttaa
    27721 tgataatttt aacgcgaatt gaatgatcat tggaaagcca acataaaatg caaaagcgac
    27781 accaaaaata aataaaagta cgctaaaaaa gctatattta taaataaatt ggcgctcatt
    27841 attatgcaaa ccaggcgcaa caaacgccca caattgataa aacataaccg gtgaaatgag
    27901 acaaaacgcg atgaaaaata taatcatcac gtatatttgg atcatttctg tgaatgaaaa
    27961 tgcatgtaag gacacatgtg cacgggtaat atacgttatg aatggtgtca tccaccaaaa
    28021 tgatgaaaca tatacgacga tgaccgtaat gacgaacgac aataaaattt ttactaaccg
    28081 atggcgtagt tcgctaaagt gaaccattaa tgtatcatca gtactattgc tctcgctgtt
    28141 gcttcgattc cttactgggt gtatcgtgag actctttatc taaatcttct gttgcagatt
    28201 taaattcttt taaagtagaa ccgatagcac gaccaaattg tggtaatttt ttcggaccaa
    28261 aaataattaa agcgataatg ctaatgacga caagacttgt tggacctgtg atgcctaaaa
    28321 taaaagtgtt agttatcatg ataatcaacc tcactcataa gtagtatata gtatttactt
    28381 tataccgaac gataatgatt atcaacaaat tttatataat aattaaaata ttgctataaa
    28441 cacaacttat tgagggatta attctgaaat aacagcttgg tcttgcaagt cgatataagt
    28501 acaagtgccc gcttgaatat cgatatgcca tttataaatg ccagcttcag ccatttcatc
    28561 acaaaatgtt tcaaaatctg tttgtccttg ttgatgtcta gttaatacat cttgaacgat
    28621 agattgattt gatttttgag caacaggaat cgtacttttc acagatgacg taacgatatc
    28681 atcttctgat tgatgtacgt atgttgcagt gccatcttga atgttgacga tattgtaagt
    28741 catccccata tctttaaaag ctttgaatag ttttggaaaa tcaacaccag taaattgttg
    28801 atgtgcttgt tgaattgcag ataatgtaaa tgccataata gtctcctttg aatcgtttga
    28861 tttagtgaaa atgcgtgcat catactaaaa ttagaatgct gttaattatg cgggaaggtg
    28921 taacgaaagt aaacaattat gacttgtagg cgttgaggtg tttgtttcaa aatgtcatac
    28981 atagctggat aatcatattt tttgtacata tttgacaaat atagttgtca aaaagacaaa
    29041 tatcatttac agtgtaatta aggagttgat gcgatgtgcg taatcgattg aaagaattac
    29101 gagcacgaga tggcttaaac caaacgcagc ttgccaaact agcgggtgtt tcaagacaaa
    29161 ccatttcgct aattgagcga aataacttta tgccatcggt attaactgca ataaagattg
    29221 ctcgtatctt caatgaaacg gtggaaactg tttttattat tgaggaggat gaggtatgaa
    29281 aatactaaga tatatcggat atcttttact aggtggactt gtagggggta tcattggtgg
    29341 aattttaggt aattttgatg gattgggtat tgagaacttg acgtttgcga catataccaa
    29401 tgtcgttgta atatcgattg ttgcgacgat tattatcata ttggtagaag ccattgtttt
    29461 gatgaatcaa agacgtgcat tgaagtataa gcgacttgta gatgaagagg tagatatcga
    29521 tgcaacagat caatatgaat tgcttgcgaa tcgttatgtt ttaaatggaa gtatattaag
    29581 cgttatacag acaattattg cctttgtagt gttactaatt tttgtggtag ggcaagctga
    29641 agcaaatgca atgctattct ttttaatacc attttttgct agtgctattt tcaatacaca
    29701 atttacactg tttaatagaa aatttgatga cagaatgcca aaaattgcag ataagaatta
    29761 cactgaaaag cgattggaaa tattggatga aggtgaacgc catatagaat taattgcatt
    29821 atttaaaaca tatgcgatca acttatcaat attgatacta gccattattt ttatagggtc
    29881 ttattcaatt gctacaggaa ttaatcaaag ctttagtttg ctacttatca ttgctatttt
    29941 catatataac gcctttagct atttattgaa gagaagacgt ttttattaaa attaaacaaa
    30001 gaggagcatg gatatgacaa cattgttaaa cgtagatcgt gtgaacaaac aatacaaaga
    30061 ttcggatttt aaattgcaag atgcatcttt aacgatttct actaatgaga cagttggatt
    30121 aattgggaaa aatggctcag gtaaatcgac attaattaat attctagtag gcaatcgaca
    30181 taaagataac ggtagtattg cattttttgg agaagaacat gctgcggatg atgtcgaata
    30241 taaagaacac ataggtgttg tgtttgatga tctgagagta cctaataaat tgactattaa
    30301 agatattgat aaagtatttc aatctattta tacgacttgg aatagtcaaa aattctttga
    30361 tttaatcaaa tatttcgagt taccacgaca aactaaaatt aaaacttttt caagagggat
    30421 gcgaatgaag atagctttaa cgattgcgct ttctcatgat gtgaagctat taatcttaga
    30481 tgaagcaact gcaggtatgg atgtttctgg acgagaagaa gtaatggaac tattagaaga
    30541 ttttgtcgct caaggtggag gcatcttaat atcatcgcat atttctgaag atatagaaca
    30601 attagcggat aaattagtgt ttatgaaaga tggacgaatg attttaactg aacagaaaga
    30661 tatactttta gcacaatatg gaattgttac gacaggagat aaagatgttg aaattactaa
    30721 gcatttaatc attgcttcta gattgtcaaa ggggaaatat caaattttag ttaaagatta
    30781 tgcagaaatt gaaaatgcag agcctttaaa acacattgat gacgctacga aaatcataat
    30841 gcgaggtgaa gtataatgaa aggtatgttc ctaagtagtt tttatgcaac gagaaagcaa
    30901 acatatattt attttatagt cgctatcata gctgcgggat actttgcagt atttaatccg
    30961 ttgatgagtt cggcaatggc tggggttatg ttaatcacac ccattactga taatattaaa
    31021 catgaaaaag actcaagatg gatgtattat gtatctactt taccggttaa acgtagtgat
    31081 tatattaagt catactttgc cttttattta atcttattcg gtgcaagttt aatgattgga
    31141 ttagttgtga ctacgatcgt gacccaaagt gtgatgattg gtattatgtc aggtttaatg
    31201 agttttggta tcataggggc atactctatc attttcccat tgacatttaa atttggcgct
    31261 gaaaactcta atgtcattat gatagttgca tctatcctac tacttttttc tttcgtggtc
    31321 tttttcttta tatatggtat ggttagcggt gcatcggcat tagaatttga aaaaattagc
    31381 actgaaggat ggttagttgt catagtatat gcggtcattg gtatcgttat aacaagtgtt
    31441 tcttatatat tgtctattaa aatttttaac aaacaagaac tataatcaat attcttggtg
    31501 tgaagctagt cattgaaata atatttagag caggtgataa atcaatacga ttatcatctg
    31561 ctctttttgg tgttgtggaa tgaaatgcgg gatgataggt tgaggtgaca tctctatatc
    31621 atatcatttg tttttagaag attaggttgt gtgggaattg catcctttta tagtgaggat
    31681 taacgtgtat atatcattga gtatgttgct gaaatttatt tgcgtgtatc aattgatgat
    31741 ggtatatgcc aatgatttcc tcggaaatat aaaaactcga tcatgtatca taactgatga
    31801 ttgtcacaaa agtataatgt gaagttcgac acttttggat tcagttcaaa tactttgacc
    31861 gaggtgaata ctatttattc atgtttatta cgtagacgtt gatttttaaa ataaatcagc
    31921 atgatgcaga aagtcgcaac ggataccgag ataatcatta cttggtcatg ccccttaaag
    31981 ataaggctga tgatatatga tactacaagt atgcctagtg aagaagaaat atattttgcg
    32041 tttgtcagtt cattatggaa ataaggcgtg attaaccata atccaatata gaatattaaa
    32101 acactgatat acatcatatt aatttcaaac aagtcattta gtttattgtt attactaaaa
    32161 acaattgcag cattaatcac acctaaagcg atattgatta atagatgcgt atacgataaa
    32221 cggaaaccga tagatgacaa tttatgatta atgtaatttt cagtaatgat ccaatataca
    32281 ccgaaaagac taattaaaat cataaattgg aatatataaa tgtaactaaa atgatcaatg
    32341 ctaaatgatg acgaagctaa accaaccagt acctcgccaa agataataat tgttagtaac
    32401 gaaaaacgtt ctactaaatg catcatatta acaggtgata atacaagata tttctgaaat
    32461 ggaataagtc ctgtcgctgc aatgaatacg cctaaaaatc cagggatgta atggatactt
    32521 tgtggtagta ctaatgatag aaatgataaa aatgaaatca caaaggctac gctcgcaaaa
    32581 gcttgacatg tacgcttatc gccataatct aaccctgtac gtatatgtaa taaatactgt
    32641 aatccgatac ttaaatacat aattgccacg cataagaaga atgggaagaa tgtcttttca
    32701 aagtccggat ataggctgtt agataggaag accatgatga acatattaaa catcataaac
    32761 gagacgtctt tgaatgtaac ttgaccaaat cgatttgtaa aaaatgtttg atgagaccac
    32821 attaaccata agaacaaact catgacgatg tatttgaaaa ataaatcagc tgaaatggaa
    32881 ccgttttgtg ttgttaaaat cacatgtgca attttttgaa tggcatagac gaaaattaaa
    32941 tcaaagaaca actcatggaa tcctgcacgc ttttcagcta aatgttttgg tgttaatgca
    33001 ttaaccataa aattttaact cctttaagat gtgtaattaa tttactaagt atactattta
    33061 ttttttctag tgaatagggg cagatttggc gatgaagtgg aaggagaggt gactgcatgg
    33121 taattgcgga attaacaatc atcagcgatt taatatttga ctagagacgt aatggtaata
    33181 aaaaattgat gagaaattga tggtgaaacc agctgtgaat agcgatgcaa tgatagatag
    33241 aatttaatta gagtcattac gcgaaatgat taatgataat ttgtggtaaa tcaaagcata
    33301 attttgtact atagatgagg atgatagagc atatttaaga gtgtgaaatg ttaaagtgaa
    33361 accgtttacg tttccgattg cccaaacaaa ttacatcatt gtataatatg atttgttaaa
    33421 tgcataacaa gaatgaaaat gtaacatacg tagcaattgg tttcataaat tggatgttag
    33481 tggcgtattg gttcattaga cgtattagta ataaaattgt atatatcata aggagatgaa
    33541 tatgacatga cgagagtcgt attagcagca gcatacagga cacctattgg cgtttttgga
    33601 ggtgcgttta aagacgtgcc agcctatgat ttaggtgcga ctttaataga acatattatt
    33661 aaagagacgg gtttgaatcc aagtgagatt gatgaagtta tcatcggtaa cgtactacaa
    33721 gcaggacaag gacaaaatcc agcacgaatt gctgctatga aaggtggctt gccagaaaca
    33781 gtacctgcat ttacagtgaa taaagtatgt ggttctgggt taaagtcgat tcaattagca
    33841 tatcaatcta ttgtgactgg tgaaaatgac atcgtgctag ctggcggtat ggagaatatg
    33901 tctcagtcac caatgcttgt caacaacagt cgcttcggtt ttaaaatggg acatcaatca
    33961 atggttgata gcatggtata tgatggttta acagatgtat ttaatcaata tcatatgggt
    34021 attactgctg aaaatttagt ggagcaatat ggtatttcaa gagaagaaca agatacattt
    34081 gctgtaaact cacaacaaaa agcagtacgt gcacagcaaa atggtgaatt tgatagtgaa
    34141 atagttccag tatcgattcc tcaacgtaaa ggtgaaccaa tcttagtcac taaggatgaa
    34201 ggtgtacgtg aaaatgtatc agtcgaaaaa ttaagtcgct taagaccagc tttcaaaaaa
    34261 gacgggacag ttacagcagg taatgcatca ggaatcaatg atggtgctgc gatgatgtta
    34321 gtcatgtcag aagacaaagc taaagaatta aatatcgaac cattggcagt gcttgatggc
    34381 tttggaagtc atggtgtaga tccttctatt atgggtattg caccagttgg cgctgtagaa
    34441 aaggctttga aacgtagtaa aaaagaatta agcgatattg atgtatttga attaaatgaa
    34501 gcatttgcag cacaatcatt agctgttgat cgtgaattaa aattacctcc tgaaaaggtg
    34561 aatgttaaag gtggcgctat tgcattagga caccctattg gtgcatctgg tgctagagtt
    34621 ttagtgacat tattgcatca actgaatgat gaagttgaaa ctggtttaac atcattgtgt
    34681 attggtggcg gtcaagctat cgctgcagtt gtatcaaagt ataaataata agaaaacagg
    34741 ttatcacaac agtattaatt acatgttggc ataacctgtt tttatttgtt tatggattta
    34801 ttgggtaata ttagtcattt gatggtttaa ttgcaaatgc tctaacaggg aacccaggtg
    34861 catcttttgg tttagggctg atagcgtaaa tgatggcgcc acgagttggt aattgatcta
    34921 aattagttaa taactcgact tggtatttat cctgaccaag aatataacgt tcgccaacta
    34981 aatcaccatt ttttacaacg tccacagatg catcggtatc gaatgtttca tgaccaacag
    35041 cttcaacacg acgttcttca attaagtact tcaaagcatc taatccccaa cccggtgcat
    35101 gttgttgtcc gttcgcatct ttgttttcaa acttttcaat attaggccaa cgttttgacc
    35161 aatcggtacg aagtgcaaca aaagtgccag gttcaatagt accatgctct ttttcccatg
    35221 cttctatatg cgcacgtgtt acaatgaaat cattgttgtt cgctacttct gttgaaaagt
    35281 ctaatacaat taacggcaat accaattctt ttaaatcaat gtcttctaaa taacgtttat
    35341 tctcgacaaa gtgaattggt gcatcaatgt gagtaccata ttgcgttaca atattccaac
    35401 gttgcacata gaaaccatga tctttaaccg tgaataaagt tgaaacttcg cctttttcaa
    35461 actcactaaa acgtggtatt tccggatcaa atgtatgcgt taaatcaacc caagttgctt
    35521 gttttaaagt atttaattgt tgccataaag gatattgtgt cataaaatca cccgttttta
    35581 gtttattata tgataaatgc tgcgattatt cttggcgttt agctttaaca gcattcacaa
    35641 gcacagtcaa tgcatcttta acttcttctt cttttcgcgt tttcaaacca cagtcagggt
    35701 ttacccagaa taatgagcgg tcgatttgtt gtagtgaacg attgattgct gtagtaattt
    35761 cttcttttgt tggaatacgt ggactatgaa tatcatacac acctagacca atacctaaat
    35821 cataattaat atcttcaaag tctttaatta aatcaccatg gctacgagat gtttcaattg
    35881 aaataacatc agcatctaag tcatgaatag catgaatgat ttgaccgaat tgagaataac
    35941 acatatgtgt atggatttga gtttcatcac gaactgaaga cgttgcaagt ttaaatgata
    36001 aaacagcatc tttaagatat tgttcgtgat attcagagcg taatggtaag ccttcacgta
    36061 atgcaggttc gtcaacttgg ataactttga ttcctgcagc ttcaagtgct aatacttctt
    36121 cgttgattgc taaagcaatt tgatcttgaa cgactttacg tggtaaatca acacgttcaa
    36181 atgaccagtt tagaattgtt acaggtccag ttaacatacc tttaactggt ttatctgtta
    36241 agctttgtgc ataaactgtt tcatcaacag ttaaaggcgc tgtccatttt acatcaccat
    36301 aaatgattgg tggttttacg gcacgtgaac catatgattg cacccaaccg aatttagtta
    36361 ctaagaaacc ttgtaatttt tctccgaaga attcaaccat gtcattacgt tcaaattcac
    36421 cgtgaactaa tacatctaag ccaatgtctt cttgaatttt aatccatcga gcaatttcat
    36481 tttttaagaa tgtttcatat gcttcgtctg taatgcgttt gttcttccaa tctgcacggt
    36541 attttcgaac ttctcggctt tgtgggaatg atccaatagt tgttgttggt aaatccggta
    36601 agttcaaacg tttttgttgt tgttcaatac gttgcgcgaa tggtgattgt cttgaagtac
    36661 gcacgctttc gaaatcataa tctaagtttt tgaatgattg attttggaaa cgctcataac
    36721 gtgcttttaa tttatcatat ttaacactat cgttttgatt aaataggcga cgcaatgcat
    36781 ctaattcgtc taatttttca gttgcaaagc ttaagccttc gccaacactt gtatctaatg
    36841 tttcatcatc taaagatact ggaacatgta ataatgaaga tgatggttga atgacaagtt
    36901 cattagtgtg tgctaacaat ttatcgatta agactttttt agcttcaatg tcacttgccc
    36961 acacattacg accatcaata attccagcgt ataatgtttt tgatttatca aaatctccag
    37021 cttcaatttg tttaaggtta tagccattat catggacaaa gtctaaacct aaaccaccaa
    37081 caggtaaaga acttaagaat ttaagatgtg cacgttcaaa gtatgtttga atgactaatt
    37141 ttttagcaac accagctttt tcgaaatagt cataagcttc acgtgtaata ttttcatagc
    37201 tttcgctgtc gtctgtaact aagattggct catcaacttg aatgtactca gcacctgcat
    37261 caattaatga ttcaaacact tctttataaa gtggtaataa cgttttaact ttttcttcaa
    37321 aagtttggtg accgcctttt gataatttaa caaaagtaat cggaccaaca atgacagggt
    37381 gagcgttaac gtttaaagat tgggcgtatt taaagcgatc taataataca ttgcgactca
    37441 ctttaggctc aacattgtcc cattcaggta cgatgtaatg atagttagtg ttaaaccatt
    37501 ttataagtgc acttgcaaca tggtctttat taccgcgagc aatatcaaat aataagtcat
    37561 catcgatagt tctaccttgg aaacgttctg ggatgatgtt gaataataat gacgtatcta
    37621 aaatatggtc atataaagag aaatcaccaa ctgggatgct atctaagtga tagtactttt
    37681 gtaataataa attttcttta tgtagatcag ttaatgtttg atctaattct tctttagaaa
    37741 tcttttttgc ccaataactt tcgatggctt ttttccattc tctttttcta cctaatcttg
    37801 ggaatcctaa gtttgatgtt ttaattgttg tcataatatt gcctccttgt gagcagtaat
    37861 agattttgag tatgctgcaa gttctaatga atcttcgaca ttttgaaacg gtgtgataat
    37921 gtataaacca ttaaaatatt catgaacagt atcgattaaa tcctttgaaa gcttaagact
    37981 tagctctcgt gttttggcgt tatcatcttt aactgcttca aattgttgta aaatttcatc
    38041 tgacatcttg attcctggca cttcattatg caaaaagagt gcgtttttgt aacttgcgat
    38101 aggcataatg cctatgaaaa atggtttgtt caagtgctta gtggcatggt aaatttcaat
    38161 gattttctct ttgctgtaca cgggttgtgt tataaaataa gacattccgc tttctatctt
    38221 tttctctaat cttttgacgg caccatctaa ttttcgaaca ttaggattaa aggcgccagc
    38281 gatgttgaag tgtgtacgtt tcttcagcgc atcaccgtca gtgttaatac cttgattaaa
    38341 tcttagagcg agttcagtta atcctttaga attaacatca tagacattgg ttgcacctgg
    38401 taagtgacca acttttgaag gatcaccagt tatggctaat atttcgttaa cgccaatgag
    38461 cgataatcca agtaaatggg actgcaagcc gattaagttt cggtctcgac atgtaatatg
    38521 tacgagtggt tcaatattgt aatattgttt aattaagctt gcagcagcaa tgttgctaat
    38581 tctgacagtt gccaatgaat tatctgcgag tgttaccgca tctacattag ctttgtcaag
    38641 tttagcgata ttttcaaaaa atctatccgt gtctaaatgt ttcggtgtat ccaattcgat
    38701 aataacggtt ggacgttctt gaaccttaga tgttaatgat tgtctaactt tattttgaga
    38761 tggattgaaa agtgctttcg ttggtatcgg aatcactttt ttgtcattaa caggtttaag
    38821 tgtctgaata gattctttaa taaatttgat gtgctctggc gttgtaccac agcaaccacc
    38881 aattaaacga acaccttcgt gaattaaatt ttgagcaact tggccgaaat attgtgcatt
    38941 gtcactatac ttaaattcac tattttcaat atctaataag ctggcatttg gatagcaaga
    39001 taagtatgcg tgctctggta attcaatatg tgtgaaagac tcttgcatat ggtgcgggcc
    39061 atgatgacaa ttgagtccca cgatgttggc accacattga acgagttgtt ttaatccttc
    39121 attgattgcc tgaccattaa ctaagtaatt tgtgtttgaa gcggttaatt gagcaatgat
    39181 tggaatgtcg tatttctttc tcgttcgtga aatgacattt gttaactctt ctaggtcgta
    39241 atacgtttcg aaaagtagcg cgtcaacgcc ttcttcaatt aaggtgtcta tttgaatttc
    39301 agtatgataa agaatagttt gtaagctgat atcctcttgt ttgatacctc taaacccacc
    39361 aactgtgcct aatatatacg tatctttatt tgctgctttt tttgcgatgc gtacggcggc
    39421 ttgatgtata gctttaactt tatcttcaag accgaatcgt tttaactttt caaaatttgc
    39481 accataagta ttggtttgaa tgacatcagc accagcttcg atatatgaac gatggatacg
    39541 ttcaacttta tctggatggc taagattata tgcttctgga caggtgtcta atccttcaga
    39601 gtataaaatg gttccaatag cgccatcagc tactaaaaca ttatctttca attgtgtgag
    39661 gaattgactc attgaatgcc tcctttaatg cgtatttgat gtctgcaatg agttcatcag
    39721 gatcttcgag accaacactt aatcggaata gaccgaaagt gataccacgt tcttgtctca
    39781 cttcttcagg tagtgcagcg tgagacattg ttgctggatg tgaaaggatc gtttcaacac
    39841 cgcccagact cactgaaacg agtggtaatg tcagtgcatc gacaaattgt tgtgctttag
    39901 actcatcagc taaacgaaag ccaataacgg caccgccatt tttagcttgt tctaaatgag
    39961 cagtagtgag tcccggataa taaacttctg aaatttcatc ttgctttatt aaaaatgaca
    40021 cgattttttg cgcgttttcg acagattgtt taaatctgat tggaaatgtt tttaaatgtt
    40081 tagcaagtgt ccagctatct tgagcagata acatattgcc tgtaccattt tgtattaaat
    40141 aaagagcttc actaattgcc tcattattag taatgacagc accagcaatt aaatcgctat
    40201 gtccacttaa aaattttgta gcactatgaa tgacaatatc agcgccaagt aataaaggtg
    40261 attggcctaa cggtgtcata aatgtattgt ccacagctac cagtagttca tgcttttcgg
    40321 ctattttaga aacagctttg atatcagtaa ttttaaaaca gggattcgat ggtgtttcga
    40381 tataaattaa ttttgtgttt gattgaatgg caccctcgat ttgttcgagc ttggtagtat
    40441 ctacggttgt aaattcaata ttaaatcgat tcaaaatttg ctcagtgagg cgaaaagtac
    40501 cgccatatac atcatcgggt aagatgacat gatcaccaga tttgaaagtc aaaagtactg
    40561 ctgaaatagc agcaatacct gatgcaaaag caaaagcgaa ttttccctgt tctaatcgtg
    40621 ctaacttctc ttctaaaagt tcacggttag ggttgccact tcgtgcataa tcatatttaa
    40681 catcgccacc aagacttgtt tgatggaatg ttgaagaatc atagagtggt gggttagctg
    40741 aatgatattc cacacctcta cgccaatcga atatcacttc tgtctctttt gaaagtgtca
    40801 tacaatctct ccaatctgag ctttatctaa tgcttggatg atatcgcgtt cgatgtcttc
    40861 ataattttca acacctagtg ataagcggat taaatactca tcaatgccac gtttatcttt
    40921 ttcagcatct ggcatatcaa catgtgtttg ggtgtaaggg aaggtcacta atgtttcagt
    40981 acctcctaaa ctttctgcaa aaatgcaaat gtctaaattt tctaataatt tagcgacgct
    41041 ataggccttg ttaagtctta aactaagcat gccagtttgc ccgctatata gtacttcgtc
    41101 aattgcttga agtgactgac attttttagc aagttttcta gcgtttgatt gcgcacgctc
    41161 aatgcgtaaa tgcaaagttt taagtccacg taacaacaaa taactatcta ttggtgaaag
    41221 tgttgcgcca gtcatgttgt gaaaatcaaa caactgttgc gcgagtgatt catctttgac
    41281 ggttacgaca cctgctagta catcgttatg tccgccaata tatttcgtgg ctgaatgtaa
    41341 gactatatca gcaccttctg ctagtggtgt tgaaagataa ggtgttaaaa aagtattgtc
    41401 gataattgac aataagcctt tagctttaca aagttgatag tatggcttta catcaatagc
    41461 aatcatttgt gggttagata ttggttcaat gaataatgca actgttttat cagtgatttc
    41521 tttttcaact tgttcataat ctgtaaaatc aacgtactta aatttgatat tgtattgttg
    41581 ctcataaaat tcaaataatc taaatgtgcc gccgtataaa tcgaatgaaa ctaaaatttc
    41641 atcgtgtggt ttaaataaat tacatattaa ttgaatggct gacattccac ttgatgtagc
    41701 gaatgatgca ataccatgtt cgagtttggc aaaacaggtt tcaaatgttg agcgtgtagg
    41761 atttttagta cgtgtataat caaaacctgt cgattgtcct agttttggat gcttgtaggc
    41821 agtagataaa tggattggat tcgctatagc accggttgaa tcatcggtta atgtgatttg
    41881 ggctaactgt gtatccttca tattaagacc ctcctataag aaaaaataaa aaaagcttcc
    41941 gtccttcgta cccgaatgaa tcggataaaa aggacgaaag cttatgtttc gcggtaccac
    42001 ctttatttgt tattccatcg ctgaaataac cttattcagt acgcattaaa agtaaatatg
    42061 cttactgaac aattatcaca attaaagtca gtaagtaagg atatagtaat gtgctatccc
    42121 atacttatta acaaaaaatc gtgcgtaaag aatccagtac gccatttaac atcaatgtta
    42181 atactgtatc gctataacgg gcgaacccgt agacacctca tattggcatc aacactccaa
    42241 ggccattttc aaacacgctt tcaaaatctt ctctcagcta ctaaagactc tctgtataag
    42301 cagggtgtgt tttactttcc tctttattgt gtttacgttt cattaaactg ttataagata
    42361 ttaattagct tacagagtaa aaaaagattt gtcaacaatt attcagaaaa ttttgattta
    42421 aaagttaatt tgtttgtgaa attgtaattg gtatcttgaa gttgaaaaat gaattatttt
    42481 ttaaataaag tgtggttaat ggttgtctga ctcatttaga atacataaaa tatatttaac
    42541 tgttgttatc aaataaaaag tgatgtgagt gaattgtcaa aaagtgaaga tcaacgtatt
    42601 actaaaacaa aagatgaaca aattaagcaa atagatatat cggatatcaa accgaatccg
    42661 tatcagcccc gaaaaacttt cgatgaaaat catttaaatg atttggcaga ttcaattaag
    42721 caatatggaa ttttgcaacc aattgtgctt agaaaaacag ttcaaggtta ttacattgta
    42781 gttggtgaaa gaaggtttag agcttcgaaa attgctggtc taaaatacgt atcagcgatt
    42841 atcaaagatt taacagatga agatatgatg gaactggcgg tcatcgaaaa tttacaacga
    42901 gaagacttaa atgcgattga agaagctgaa agttatcaac gtttgatgac agatttgaaa
    42961 attacacaac aagaagtagc gaagcgattg agtaagtcgc gcccgtatat agcgaatatg
    43021 ttgaggttat tacatttgcc gaaaaagatt gctgacatgg taaaagatgg gcgactgaca
    43081 agtgcacatg gacgaacgtt attggcaatt aaagatgaac aacaaatgct taggttagcg
    43141 aaacgggttg ttaaagaaaa gtggagtgtt agatatttag aaaaccatgt taatgaatta
    43201 aaaaatgttt cgtcaaagtc ggaaacagac aaagtagata taactaagcc taaatttata
    43261 aagcaacaag aacgacagtt gcgagaacag tatggtacca aagtagatat atcaataaaa
    43321 aaatcggttg gtaaaatctc atttgagttt gattcacaag aagattttct aagaataatt
    43381 gaacaattaa atcgtaggta tggtaaatag ttacacaatt ttatataata actctttgtg
    43441 caagtgtaaa taaattgtaa tcagtgaaca tttgattcta gatatattga gactttcgta
    43501 ggttggaagt atataaaaat atgaatacaa tattgatata tcatatagaa gggagtaacg
    43561 cttatcatga atcaagtcat gaatattatt tcatctctat ttgagccatt aacaaaaata
    43621 gaaacatatg aaaacattgc aactaaaatc gctatgattg ttatttatat tatcgtagcc
    43681 ctcatagtta ttaaaatact gaataaaatg attgaacagg gatttaagat tcaaaataag
    43741 agtaaaaaga gtaacaaaaa gcgctctaaa actttaatat ctcttgttca aaatgtagtg
    43801 aagtatatcg tttggtttat agttattacg acgattttaa gtaaatttgg cattagtgtt
    43861 gaaggtgtta ttgcaagtgc tggtgtcgta ggtttagcag taggttttgg tgctcaaacc
    43921 attgttaaag acgttattac tggatttttc attatatttg aaagtcaatt tgatgtaggt
    43981 gattatgtta aaataaacaa tggtggtacg actgtggcag agggaacggt taaatcaata
    44041 ggacttcgtt caacacgaat caatacaatt tcaggagaat taacaatttt accaaatagt
    44101 agtatgggtg aaataacgaa ctactcaatt acaaatggta cagctatcgt taaaattcca
    44161 gtgtctgtcg aagaaaacat tgataatgtt gataaaaaac taaacaaact atttacttct
    44221 ttacgtagta aatattactt atttgttagt gatccggttg ttattggtat tgatgctatt
    44281 gaagatacaa gagtaatatt gagaatatct gcagaaacaa ttccaggtga aggatttgct
    44341 ggagctcgaa ttattcgcaa agaagtacaa aaaatgtttt tacaagaagg tattaaaaca
    44401 cctcaaccaa ttatgactgc ttataatcat agtgaaaacg gtgtttagta gtttataata
    44461 catggaggtc atatttaatg gcgtcaaaat atggaataaa tgatatagta gaaatgaaaa
    44521 aacaacatgc gtgtggaaca aaccgtttta agattattag aatgggtgca gacataagaa
    44581 ttaaatgtga aaattgtcaa agaagtatta tgattccacg tcaaacgttt gataaaaaac
    44641 ttaaaaaaat catcgaatct catgatgata cacaaagata ggagaatgat taatggcttt
    44701 aacagcaggt atcgttggat tgccaaacgt tggtaaatca acattattta atgcaataac
    44761 aaaagcaggt gctttagcag cgaactatcc attcgctacg attgatccta atgtagggat
    44821 agtagaagtg ccagatgcta gattacttaa attagaagaa atggttcaac ctaaaaagac
    44881 attgccgact acatttgaat ttacagatat cgctggtatt gtgaaaggtg cttcaaaggg
    44941 agaagggtta ggtaataaat tcttatcaca tattagagaa gtagatgcga tttgtcaggt
    45001 cgttcgtgca tttgatgatg ataacgtaac tcatgttgct ggtcgagtag accctattga
    45061 tgatattgaa gttattaata tggaattagt actagcggac ttagaatctg ttgagaaacg
    45121 tttgcctaga attgaaaaat tagcacgtca aaaagataag actgctgaaa tggaagtacg
    45181 tattttaaca actattaaag aagctttaga aaatggtaaa cccgctcgta gtattgactt
    45241 taatgaagaa gatcaaaaat gggtgaatca agcgcaatta ctgacttcta aaaaaatgct
    45301 ttatatcgct aatgttggtg aagatgaaat tggtgatgat gataatgata aagtaaaagc
    45361 gattcgtgaa tatgcagcgc aagaagactc tgaagtgatt gttattagtg caaaaattga
    45421 agaagaaatt gctacattag atgatgaaga taaagaaatg ttcttagaag atttaggtat
    45481 cgaagaacca ggattagatc gattaattag aacaacttat gaattattag gattatcaac
    45541 atattttact gctggtgtgc aagaagtacg tgcttggaca tttaaacaag gtatgactgc
    45601 acctcaatgt gctggtatca ttcatactga ttttgaacgt ggatttatcc gtgccgaagt
    45661 aacaagttat gacgactatg tacaatatgg tggcgaaagt ggcgctaaag aagcgggcag
    45721 acaacgatta gaaggtaaag aatatattat gcaagatggt gatatcgttc atttcagatt
    45781 taatgtataa acgatagagt gaagttaatt aaatagtata tatggagaag aggcggaatc
    45841 aattgttcgc ctcttttaat tatgcgtata atttattaaa agaatggaat aattttactc
    45901 gcgttaataa tatcttgagt gctgaaaaat tgtttgcctt cgccagtata agcaggctct
    45961 aaaacaagat tagcctttgc acaataaagc cattcaggat gaatgccact attaagtatc
    46021 tcttggaatt cttgaaaatc tttagaccaa tcaatattta aattcattcc ttaccacctc
    46081 acaactatta tagaacacgt gttcgctttt gtgaagtgta tttttaaaaa tatcatagaa
    46141 aatttcaaat gaattaatgt caaaaatgat atgaagagta gtttgaatta ttattgtaaa
    46201 aagtaatggc gcatgatata attctttatt gtgagtaatg aaaattattc cttgcttatc
    46261 tgttttaaga ttgataagcc gtatagacca caaggaggtg caaatataaa atgagaacat
    46321 atgaagttat gtacatcgta cgcccaaaca ttgaggaaga tgctaaaaaa gcgttagttg
    46381 aacgtttcaa cggcatctta gctactgaag gtgcagaagt tttagaagca aaagactggg
    46441 gtaaacgtcg cctagcttat gaaatcaatg atttcaaaga tggcttctac aacatcgtac
    46501 gtgttaaatc tgataacaac aaagctactg acgaattcca acgtctagct aaaatcagtg
    46561 acgatatcat tcgttacatg gttattcgtg aagacgaaga caagtaataa ttagaggggg
    46621 cgtttaaatg ctaaatagag ttgtattagt aggtcgttta acgaaagatc cggaatacag
    46681 aaccactccc tcaggtgtga gtgtagcgac attcactctt gcagtaaatc gtacgttcac
    46741 gaatgctcaa ggggagcgcg aagcagattt tattaactgt gttgttttta gaagacaagc
    46801 agataatgta aataactatt tatctaaagg tagtttagct ggtgtagatg gtcgcttaca
    46861 atcccgtaat tatgaaaatc aagaaggtcg tcgtgtgttt gttactgaag ttgtgtgtga
    46921 tagtgttcaa ttccttgaac ctaaaaatgc gcaacaaaat ggtggccaac gtcaacaaaa
    46981 tgaattccaa gattacggtc aaggattcgg tggtcaacaa tcaggacaaa acaattcgta
    47041 caataattca tcaaacacga aacaatctga taatccattt gcaaatgcaa acggaccgat
    47101 tgatataagt gatgatgact taccattcta ataaaaatta acgaaattaa agcgaaaaaa
    47161 ttatcaaagg aggcacacaa tcatggcagg tggaccaaga agaggcggac gtcgtcgtaa
    47221 aaaagtatgc tatttcacag caaatggtat tacacatatc gactacaaag acactgaatt
    47281 attaaaacgt tttatctcag aacgcggtaa aattttacca cgtcgtgtaa ctggtacttc
    47341 agctaaatat caacgtatgt tgactacagc tatcaaacgt tctcgtcata tggcattatt
    47401 accatatgtt aaagaagaac aataatatat aatttattgt caaaccccgt aggcataggc
    47461 ttacggggct ttttgtgttt tggggtatag aaaaagggca aaaaaggatg atgtgaatgt
    47521 tttgtgttcg gaatttgcac aaagatatgt ttatattgca aaaataatat gaatttagat
    47581 gcataaaaaa agaactacgc attttaaata aaatgcatag ctcttctttt tcttgcatac
    47641 gaattaaaat aactcgcgag acctataagt ctctttcctc actagatagt ttatactttt
    47701 ggtctgttga agtcaataat tttatctaaa gctataaaaa atcttttgat agctaatgca
    47761 ttattataat agctttcgtt tcttttatat cgcttttgaa gttggtccaa atcgtgatat
    47821 cttgcttgga taattgcatt actacaaact tgattatgta attctagcgt agcgaaagta
    47881 tctatgaaat tttttattcc gaacatgttt ctagatatgc ctatattatt ccctttttca
    47941 aataaatatt gaggagatct actgtcataa tttagatttg ctatgatggg ttggttatga
    48001 gctgatttgt ttcttatatt tttaactaaa ggcattaaaa tattagcaac tctcaattct
    48061 tcgtcattgt acttcttgta atagaagttg agaaacgaaa cgaattgacc tagttgcatg
    48121 aattcaatgc aaacccatgc gggtggattt tgatagtatt tattcaactt ctcgggtagt
    48181 tgtcctcgtt tattcatatg cttgaatatt tcgtttttat ttttgatttt ggtttccata
    48241 acttcttctg gtgttcttga atttgtgtca aaatttgaat tgctatatga tttatcaata
    48301 cataagaact catctattat tttataacca tcttcttggt tattttctgt tattagtttt
    48361 aagactagac actttaaact atgttcaata tctaaagtta aatgcaacat tgtgtatctt
    48421 aatttcatat ctatagttgc taaatctgat aaataagcaa attctatgaa atagccgcca
    48481 ttcttttttt cgaaattttt tcggaaataa gctagtttga agaagtgatt attttttcta
    48541 agaatttcat ttgctttttc ggtgtcaata atattaaaaa atatattcat ctgttttaat
    48601 ttcgctattt gctcatcaaa attgagcata ggcttaattt ctgctagtgt atcatctttt
    48661 tccaatttta actccccaat cattcaaatt tattcatcat atctttcgcc atctgattag
    48721 taacatgtgt gtatatctct agattttttt ataatctgaa tgacctacat gctcttgcat
    48781 tgcttttaag ttaattccta attgagcaag tgtagatata tgcgaatgat gtaatgtatg
    48841 cgtcgttaca ggtttcttaa tagaactaat ctcagttgtc tctttgatag tgcccccttt
    48901 aataatgttg ctaattttgt tcgagtcgat agggccacca gctgcatttg tgaatatgta
    48961 ccctatatta ataaacttat cattacaagc ataagtgttc ttagtaagtc gatgctatgg
    49021 atagtgaggc ctatagccct atagctgtta cttgttctag ttgtctcttt tactccggat
    49081 gctcccgtca tttttcagtt atccaattaa ctttaccgtc gatatctagc gttttatctt
    49141 catagtttat atttactctc tttattgcaa gtagctcacc gatacgcatg tcattagcaa
    49201 tttgaaactg taccatagct tttaccattt tataattacg ttttgtcgtt ggatattttt
    49261 atacttaatt acatagtcga aacaatccag taactcctta tcttcattat cttctaaagt
    49321 gttattacgt ttagctagta acgcatcact atagggatat ctattttatc tattacacat
    49381 ataactttga attgcttact attttaaatt aacaaattct attctcttag atttagtcca
    49441 attatgtgta gacgatttat agttattaaa ttcagagtgg tagcaaatta aagttaatca
    49501 agagttaaga tgaatttaat tcatgagcac gtctattatt tttataattg aagaaaataa
    49561 agttttactt caaggaggta attaaatatg ttcaaaaaaa atgactcgaa aaattcaatt
    49621 ctattaaaat ctattctatc gctaggtatc atctatgggg gaacatttgg aatatatcca
    49681 aaagcagacg cgtcaacaca aaattcccca agtgtacaag ataaacaatt ccaaaaagtt
    49741 gaagaagtac caaataattc agaaaaagct ttggttaaaa aactttacga tagatacagc
    49801 caaaatacaa taaacggaaa atctaataaa tctaggaatt gggtttattc agagagacct
    49861 ttaaatgaaa accaagttcg tataaattta gaaggaacat acagagttgc tgatagagta
    49921 tatacaccta agagaaatat tactcttaat aaagaagttg tcactttaaa ggaattggat
    49981 catatcataa gatttgctca tatttcttat ggcttatata tgggagaaca tttgcctaaa
    50041 ggtaacatcg tcataaatac aaaagatggc ggtaaatata cattagagtc gcataaagag
    50101 ctacaaaaag atagggaaaa tgtaaaaatt aatacagccg atataaaaaa tgtaactttc
    50161 aaacttgtga aaagtgttaa tgacattgaa caagtttgaa attaagttga attagtatat
    50221 acagtgtttt attgctaata ctttgaaagt taggtatcta aaggtgccta actttctttg
    50281 ttatgattag caccatcata tagaaaattc tttaatgacg tttatgccga aatctacaaa
    50341 aataatttct cttttatggt taatcaatat gtttttacat tcgacaacct taaaattaac
    50401 ttctttcaat tccataataa agtctctata aaataactta gtttaaaaac gattcatatc
    50461 tttcagattc aaataccatc attttctact aatacttaca ctttaattac aattatgtaa
    50521 gttgttttca gagacatcct attttatatt ttgtcagagg tgatcttttg aaaacgattt
    50581 taaaaacaat aacatatcta gcacttacta tcattggcgc ttatgctgct ttattcattt
    50641 taaaaacaat agactctcat ggtataacag atcaatttaa cccattagta aaggaagatg
    50701 tttcttatgt taaaacgact gaggtgtcta ctagaatgga tgatcaactc cgaagttata
    50761 gtcaaagtgc ttttaataaa gaagggaaag agacgcaatt aatgtatact gctacatttg
    50821 atgttaaacc gcatagatac ttgaaaatta cacataaagg tcatcacgta gaaacttttg
    50881 aagaagttga aaaggggcaa gtacctaaaa aaagcattag acaaattgag tcgattatag
    50941 cgtgcttata ttacatggtt caattataaa atgagtacga acgaaagtaa cacatgatga
    51001 ttaatttaaa aatatttgta ataattatga ataaaattaa aaacaagggg taatacaatc
    51061 tatatagcat ataagctttt gttatgagtt tcaaaaaagg aagagagagt gatattatga
    51121 aattaaaatc attagcagtg ttatcaatgt cagcggtggt gcttactgca tgtggcaatg
    51181 atactccaaa agatgaaaca aaatcaacag agtcaaatac taatcaagac actaatacaa
    51241 caaaagatgt tattgcatta aaagatgtta aaacaagccc agaagatgct gtgaaaaaag
    51301 ctgaagaaac ctacaaaggc caaaagttga aaggaatttc atttgaaaat tctaatggtg
    51361 agtgggctta taaagtgacg caacaaaaat ctggtgaaga gtcagaagta cttgttgctg
    51421 ataaaaataa aaaagtgatt aacaaaaaga ctgaaaaaga agatacagtg aatgaaaatg
    51481 ataactttaa atatagcgat gctatagatt acaaaaaagc cattaaagaa ggacaaaaag
    51541 aatttgatgg tgatattaaa gaatggtcac ttgaaaaaga tgatggcaaa cttgtttaca
    51601 atatcgattt gaaaaaaggt aataaaaaac aagaagttac tgttgatgct aagaacggta
    51661 aagtattaaa gagtgagcaa gatcactaaa aagattcgtt gcattaacgc ttgtaaaatg
    51721 tcttaattca tattaattat taaaataccc tcacaacgca atgtaatata tccagtatag
    51781 atttgaaagg atatatgcct aacgtgttgt gagggtttat ttgcatctat ttatccatgc
    51841 gaatatcgac ttcttctaaa tgtttctgat attctttaac cttactttct aaaaacattt
    51901 catatggtgc atcaaagaaa tcagctaaat gcatggcgtc tttaaattta ggttcatggt
    51961 gatgattctc ccattcccaa atttgatgtg cttcatattt agtaccatat ttctcattta
    52021 attgttgtgc taattcgtca atttctaaat tatgtttagt tcgtaagtta tataaaatat
    52081 gcatattcat tgttcattaa cctcgcttct tgatttaaaa ttcaaagatg ctctaataat
    52141 atgtgtatat tctagtccta taacataaaa taatcaagct atgaattcgg cagaaaaaat
    52201 aatgtatatg atattgccgg tacagagact ttaaataacg atagctacat tgaataaaat
    52261 tgatattcaa ttactacttt taaaaatatt tggataaaaa taatttgaat ttgttttaga
    52321 attgtaaata aggggtacta cttagaataa cgcaaaataa atagaaaaag gagactgaaa
    52381 attatgtttg gatttattgg aatgttaatt atcggtggct taattggatg ggctgctggt
    52441 gctattatgg gtaaagatat cccaggtggt attttaggca atattatcgc aggtattatt
    52501 ggatcatggg taggtggcaa actattcgga caatggggtc ctgaattagg aagtatttac
    52561 atcttgccag cattaattgg ttcaattatc ttaattgcaa tcgtaacgtt aattttaaga
    52621 gctatgcgta aataataaaa aaattcaatt ggaaattgaa ataaagatgg cattcagttg
    52681 tcatcttttt tatttgcgtg aaaatggtca atatgtatta taatatttga aaatgtagat
    52741 aagataggat taaaaagtaa taaattaata tgaggaaatt ttggaaataa aaaagcgagt
    52801 atttgacaaa taaaaaaagg atgacgttgg gttgcgccat ccaggagtat tatgttcatg
    52861 aatataaaag actgtgtagt catttaagaa ctacacccat atagtaacac atcacttgat
    52921 taaagacaat actattttta gagtcattaa ttt
//
LOCUS       DAMZKC010000002        43144 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.002, whole genome shotgun sequence.
ACCESSION   DAMZKC010000002 DAMZKC010000000
VERSION     DAMZKC010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 43144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43144
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.002"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(439..511)
                     /locus_tag="POB55_000057"
     tRNA            complement(439..511)
                     /locus_tag="POB55_000057"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(476..478),aa:Asp,seq:gtc)
     gene            complement(519..593)
                     /locus_tag="POB55_000058"
     tRNA            complement(519..593)
                     /locus_tag="POB55_000058"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(558..560),aa:Glu,seq:ttc)
     gene            complement(1023..2306)
                     /locus_tag="POB55_000059"
     CDS             complement(1023..2306)
                     /locus_tag="POB55_000059"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HDI6499057.1"
                     /translation="MSSIVVVGTQWGDEGKGKITDFLAEQSDVIARFSGGNNAGHTIQ
                     FGGETYKLHLVPSGIFYKDKLAVIGNGVVVDPVALLKELDGLNERGIPTSNLRISNRA
                     QVILPYHLAQDEYEERLRGDNKIGTTKKGIGPAYVDKVQRIGIRMADLLEKETFERLL
                     KSNIEYKQAYFKGMFNETCPSFDDIFEEYYAAGQRLKEFVTDTSKILDDAFVADEKVL
                     FEGAQGVMLDIDHGTYPFVTSSNPIAGNVTVGTGVGPTFVSKVIGVCKAYTSRVGDGP
                     FPTELFDEDGHHIREVGREYGTTTGRPRRVGWFDSVVLRHSRRVSGITDLSINSIDVL
                     TGLDTVKICTAYELDGKEITEYPANLDQLKRCKPIFEELPGWTEDVTNVRTLEELPEN
                     ARKYLERISELCNVQISIFSVGPDREQTNLLKELW"
     gene            complement(2585..3985)
                     /gene="dnaB"
                     /locus_tag="POB55_000060"
     CDS             complement(2585..3985)
                     /gene="dnaB"
                     /locus_tag="POB55_000060"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDI6499058.1"
                     /translation="MDRMYEQNQMPHNNEAEQSVLGSIIIDPELINTTQEVLLPESFY
                     RGAHQHIFRAMMHLNEDNKEIDVVTLMDQLSTEGTLNEAGGPQYLAELSTNVPTTRNV
                     QYYTDIVSKHALKRRLIQTADSIANDGYNDELELDAILSDAERRILELSSSRESDGFK
                     DIRDVLGQVYETAEELDQNSGQTPGIPTGYRDLDQMTAGFNRNDLIILAARPSVGKTA
                     FALNIAQKVATHEDMYTVGIFSLEMGADQLATRMICSSGNVDSNRLRTGTMTEEDWSR
                     FTIAVGKLSRTKIFIDDTPGIRINDLRSKCRRLKQEHGLDMIVIDYLQLIQGSGSRAS
                     DNRQQEVSEISRTLKALARELECPVIALSQLSRGVEQRQDKRPMMSDIRESGSIEQDA
                     DIVAFLYRDDYYNRGGDEDDDDDGGFEPQTNDENGEIEIIIAKQRNGPTGTVKLHFMK
                     QYNKFTDIDYAHADMM"
     gene            complement(4017..4463)
                     /gene="rplI"
                     /locus_tag="POB55_000061"
     CDS             complement(4017..4463)
                     /gene="rplI"
                     /locus_tag="POB55_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HDI6499059.1"
                     /translation="MKVIFTQDVKGKGKKGEVKEVPVGYANNFLLKKNYAVEATPGNL
                     KQLELQKKRAKQERQQEIEDAKALKETLSNIEVEVSAKTGEGGKLFGSVSTKQIAEAL
                     KAQHDIKIDKRKMDLPNGIHSLGYTNVPVKLDKEVEGTIRVHTVEQ"
     gene            complement(4460..6427)
                     /gene="gdpP"
                     /locus_tag="POB55_000062"
     CDS             complement(4460..6427)
                     /gene="gdpP"
                     /locus_tag="POB55_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP phosphodiesterase GdpP"
                     /protein_id="HDI6499060.1"
                     /translation="MNRQSTKKALLIPFVIMIITAIVLMGVWFIFNSLIALIASIVLV
                     VMIIVSIVLFRQALMKMDSYVDGLSAQISTTNNKAIKHLPIGIIVLDENDHIEWVNQF
                     MTDHMEANVISESVNEVFPNILKQLDRVKSVEIEYNQYHFQVRYSENDHCLYFFDITE
                     QVQTNELYENSKPIIATLFLDNYDEITQNMNDTQRSEINSMVTRVISRWATEYNIFFK
                     RYSSDQFVAYLNQKILADLEESKFDILSQLREKSVGYRAQLTLSIGVGEGTENLIDLG
                     ELSQSGLDLALGRGGDQVAIKSINGNVRFYGGKTDPMEKRTRVRARVISHALKDILAE
                     GDKVIIMGHKRPDLDAIGAAIGVSRFAMMNNLEAYIVLNETDIDPTLRRVMNEIDKKP
                     ELRERFITSDDAWDMMTSKTTVVIVDTHKPELVLDENVLNKANRKVVIDHHRRGESFI
                     SNPLLIYMEPYASSTAELVTELLEYQPTEQRLTRLESTVMYAGIIVDTRNFTLRTGSR
                     TFDAASYLRAHGADTILTQHFLKDDVDTYINRSELIRTVKVEDNGIAIAHGSDDKIYH
                     PVTVAQAADELLSLEGIEASYVVARREDNLIGISARSLGSVNVQLTMEALGGGGHLTN
                     AATQLKGVTVEEAIAQLQQAITEQLSRSEDA"
     gene            complement(6442..7380)
                     /locus_tag="POB55_000063"
     CDS             complement(6442..7380)
                     /locus_tag="POB55_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HDI6499061.1"
                     /translation="MGVAYVFSKIQPKATIIATIALVFVALALYLVPGLGLIFALFAT
                     IPGIVLWNKSIQSFGISALITVIITTVLGNTFVLSAIILVLIASLIIGQLLKERTSKE
                     RILYVTTVAMSLISLIAFMLLQTFGRIPPSASIVKPFKQTLHEAITMSGADANMTQIL
                     EEGFRQATVQLPGFIIIITFLIVLINLIVTFPILRKFKIATPVFKPLFAWQMSGILLW
                     IYIIVIICLLFTGQPSVFQSILLNFQLVLSLVMYIQGLSVIHFFGKAKGLPNAVTILL
                     LIIGTILTPTTHIVGLLGVIDLSLNLKRIMKNNSKK"
     gene            complement(7673..8641)
                     /locus_tag="POB55_000064"
     CDS             complement(7673..8641)
                     /locus_tag="POB55_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDI6499062.1"
                     /translation="MTNYTVDTLNLGKFITESGEVIDNLRLRYEHVGYHGQPLVVVCH
                     ALTGNHLTYGTDDYPGWWREIIDGGYIPIHDYQFLTFDVIGSPFGSSSPLNDPHFPKK
                     LTLRDIVRANERGIQALGYDKINILIGGSLGGMQAMELLYNQQFEVDKAIILAATSRT
                     SSYSRAFNEIARQAIHLGGKEGLSIARQLGFLTYRSSKSYDERFTPDEVVAYQQHQGN
                     KFKEHFDLNCYLTLLDVLDSHNIDRGRTDVTHVFKNLETKVLTMGFIDDLLYPDDQVR
                     ALGERFKYHRHFFVPDNVGHDGFLLNFSTWAPNLYHFLNLKHFKRK"
     regulatory      complement(8702..8800)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(9004..9333)
                     /locus_tag="POB55_000065"
     CDS             complement(9004..9333)
                     /locus_tag="POB55_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDI6499063.1"
                     /translation="MITHMNMLILILLCGIVTLLIRIIPFIMISKVQLPDVVVRWLSF
                     IPITLFTALVIDSIIQQTPHGEGYTLNIPYIIALIPTVILSIITRSLTITIISGIVIM
                     AALRFFF"
     gene            complement(9330..10025)
                     /locus_tag="POB55_000066"
     CDS             complement(9330..10025)
                     /locus_tag="POB55_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDI6499064.1"
                     /translation="MTTHLSFRQGVRECVPTLLGYAGVGISFGIVASSQNFSILEIVL
                     LCLVIYAGAAQFIMCALFIAGTPISAIVLTVFIVNSRMFLLSMSLAPNFKTYGFWNRV
                     GLGSLVTDETFGVAITPYLKGEAINDRWMHGLNITAYLFWAISCVAGALFGEYISNPQ
                     ALGLDFAITAMFIFLAIAQFESITKSRLRIYIVLIIAVIVMMLSLSMFMPSYLAILIA
                     ATISAALGVMMER"
     gene            complement(10677..11963)
                     /gene="serS"
                     /locus_tag="POB55_000067"
     CDS             complement(10677..11963)
                     /gene="serS"
                     /locus_tag="POB55_000067"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDI6499065.1"
                     /translation="MLDIRLFRNEPVTVKSKIELRGDDSKVVDEILELDEQRRKLISA
                     TEEMKARRNKVSEEIALKKRNKENADDVIAEMRTLGDDIKEKDSQLNEIDNKMTGILC
                     RIPNLISDDVPQGESDEDNVEVKKWGTPREFSFEPKAHWDIVEELKMADFDRAAKVSG
                     ARFVYLTNEGAQLERALMNYMITKHTTQHGYTEMMVPQLVNADTMYGTGQLPKFEEDL
                     FKVEKEGLYTIPTAEVPLTNFYRNEIIQPGVLPEKFTGQSACFRSEAGSAGRDTRGLI
                     RLHQFDKVEMVRFEQPEDSWNALEEMTTNAEAILEELGLPYRRVILCTGDIGFSASKT
                     YDLEVWLPSYNDYKEISSCSNCTDFQARRANIRFKRDKAAKPELAHTLNGSGLAVGRT
                     FAAIVENYQNEDGTVTIPEALVPFMGGKTQISKPVK"
     misc_binding    complement(12053..12269)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(12342..13856)
                     /gene="hutH"
                     /locus_tag="POB55_000068"
     CDS             complement(12342..13856)
                     /gene="hutH"
                     /locus_tag="POB55_000068"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HDI6499066.1"
                     /translation="MTLYLDGETLTIEDIKSFLQQQSKIEIIDDALERVKKSRAVVER
                     IIENEETVYGITTGFGLFSDVRIDPTQYNELQVNLIRSHACGPGEPFSKEVALVMMIL
                     RLNTLLKGHSGATLELVRQLQFFINERIIPIIPQQGSLGASGDLAPLSHLALALIGEG
                     KVLYRGEEKDSDDVLRELNRQPLNLQAKEGLALINGTQAMTAQGVISYIESEDLGYQS
                     EWIAALTHQSLNGIIDAYRHDVHAVRNFQEQINVAARMRDWLEGSTLTTRQAEIRVQD
                     AYTLRCIPQIHGASFQVFNYVKQQLEFEMNAANDNPLIFEEANETFVISGGNFHGQPI
                     AFALDHLKLGVSELANVSERRLERLVNPQLNGDLPAFLSPEPGLQSGAMIMQYAAASL
                     VSENKTLAHPASVDSITSSANQEDHVSMGTTAARHGYQIIENARRVLAIECVIALQAA
                     ELKGVEGLSPKTRRKYEEFRSIVPSITHDRQFHKDIEAVAQYLKQSIYQTTACH"
     gene            14182..14994
                     /locus_tag="POB55_000069"
     CDS             14182..14994
                     /locus_tag="POB55_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HDI6499067.1"
                     /translation="METLNSINIPKRKEDSHKGDYGKILLIGGSANLGGAIMLAARAC
                     VFSGSGLITVATHPTNHSALHSRCPEAMVIDINDTKMLTKMIEMTDSILIGPGLGVDF
                     KGNNAITFLLQNIQPHQNLIVDGDAITIFSKLKPQLPTCRVIFTPHLKEWERLSGIPI
                     EEQTYERNREAVDRLGATVVLKKHGTEIFFKDEDFKLTIGSPAMATGGMGDTLAGMIT
                     SFVGQFDNLKEAVMSATYTHSFIGENLAKDMYVVPPSRLINEIPYAMKQLES"
     gene            complement(15081..17744)
                     /gene="gyrA"
                     /locus_tag="POB55_000070"
     CDS             complement(15081..17744)
                     /gene="gyrA"
                     /locus_tag="POB55_000070"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HDI6499068.1"
                     /translation="MAELPQSRINERNITSEMRESFLDYAMSVIVARALPDVRDGLKP
                     VHRRILYGLNEQGMTPDKSYKKSARIVGDVMGKYHPHGDSSIYEAMVRMAQDFSYRYP
                     LVDGQGNFGSMDGDGAAAMRYTEARMTKITLELLRDINKDTIDFIDNYDGNEREPSVL
                     PARFPNLLANGASGIAVGMATNIPPHNLTELINGVLSLSKNPDISIAELMEDIEGPDF
                     PTAGLILGKSGIRRAYETGRGSIQMRSRAVIEERGGGRQRIVVTEIPFQVNKARMIEK
                     IAELVRDKKIDGITDLRDETSLRTGVRVVIDVRKDANASVILNNLYKQTPLQTSFGVN
                     MIALVNGRPKLINLKEALVHYLEHQKTVVRRRTQYNLRKAKDRAHILEGLRIALDHID
                     EIISTIRESDTDKVAMESLQQRFKLSEKQAQAILDMRLRRLTGLERDKIEAEYNELLN
                     YISELEAILADEEVLLQLVRDELTEIRDRFGDDRRTEIQLGGFEDLEDEDLIPEEQIV
                     ITLSHNNYIKRLPVSTYRAQNRGGRGVQGMNTLEEDFVSQLVTLSTHDHVLFFTNKGR
                     VYKLKGYEVPELSRQSKGIPVVNAIELENDEVISTMIAVKDLESEDNFLVFATKRGVV
                     KRSALSNFSRINRNGKIAISFREDDELIAVRLTSGQEDILIGTSHASLIRFPESTLRP
                     LGRTATGVKGITLREGDEVVGLDVAHANSVDEVLVVTENGYGKRTPVNDYRLSNRGGK
                     GIKTATITERNGNVVCITTVTGEEDLMIVTNAGVIIRLDVADISQNGRAAQGVRLIRL
                     GDDQFVSTVAKVKEDAEDETNEDEQSTSTVSEDGTEQQREAVVNDETPGNAIHTEVID
                     SEENDEDGRIEVRQDFMDRVEEDIQQSSDEE"
     gene            complement(17781..19715)
                     /gene="gyrB"
                     /locus_tag="POB55_000071"
     CDS             complement(17781..19715)
                     /gene="gyrB"
                     /locus_tag="POB55_000071"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HDI6499069.1"
                     /translation="MVTALSDVNNTDNYGAGQIQVLEGLEAVRKRPGMYIGSTSERGL
                     HHLVWEIVDNSIDEALAGYANQIEVVIEKDNWIKVTDNGRGIPVDIQEKMGRPAVEVI
                     LTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSQDLEVYVHRNETIYHQAYKKGVPQF
                     DLKEVGTTDKTGTVIRFKADGEIFTETTVYNYETLQQRIRELAFLNKGIQITLRDERD
                     EENVREDSYHYEGGIKSYVELLNENKEPIHDEPIYIHQSKDDIEVEIAIQYNSGYATN
                     LLTYANNIHTYEGGTHEDGFKRALTRVLNSYGLSSKIMKEEKDRLSGEDTREGMTAII
                     SIKHGDPQFEGQTKTKLGNSEVRQVVDKLFSEHFERFLYENPQVARTVVEKGIMAARA
                     RVAAKKAREVTRRKSALDVASLPGKLADCSSKSPEECEIFLVEGDSAGGSTKSGRDSR
                     TQAILPLRGKILNVEKARLDRILNNNEIRQMITAFGTGIGGDFDLAKARYHKIVIMTD
                     ADVDGAHIRTLLLTFFYRFMRPLIEAGYVYIAQPPLYKLTQGKQKYYVYNDRELDKLK
                     SELNPTPKWSIARYKGLGEMNADQLWETTMNPEHRALLQVKLEDAIEADQTFEMLMGD
                     VVENRRQFIEDNAVYANLDF"
     gene            complement(19725..20837)
                     /gene="recF"
                     /locus_tag="POB55_000072"
     CDS             complement(19725..20837)
                     /gene="recF"
                     /locus_tag="POB55_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HDI6499070.1"
                     /translation="MKLNTLQLENYRNYDEVTLKCHPDVNILIGENAQGKTNLLESIY
                     TLALAKSHRTSNDKELIRFNADYAKIEGELSYRHGTMPLTMFITKKGKQVKVNHLEQS
                     RLTQYIGHLNVVLFAPEDLNIVKGSPQIRRRFIDMELGQISAVYLNDLAQYQRILKQK
                     NNYLKQLQLGQKKDLTMLEVLNQQFAEYAMKVTDKRAHFIQELESLAKPIHAGITNDK
                     EALSLNYLPSLKFDYAQNEAARLEEIMSILSDNMQREKERGISLFGPHRDDISFDVNG
                     MDAQTYGSQGQQRTTALSIKLAEIELMNIEVGEYPILLLDDVLSELDDSRQTHLLSTI
                     QHKVQTFVTTTSVDGIDHEIMNNAKLYRINQGEIIK"
     gene            complement(20834..21079)
                     /gene="yaaA"
                     /locus_tag="POB55_000073"
     CDS             complement(20834..21079)
                     /gene="yaaA"
                     /locus_tag="POB55_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HDI6499071.1"
                     /translation="MIILVQEVVVEGDINLGQFLKTEGIIESGGQAKWFLQDVEVLIN
                     GVRETRRGKKLEHQDRIDIPELPEDAGSFLIIHQGEQ"
     gene            complement(21460..22593)
                     /gene="dnaN"
                     /locus_tag="POB55_000074"
     CDS             complement(21460..22593)
                     /gene="dnaN"
                     /locus_tag="POB55_000074"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HDI6499072.1"
                     /translation="MMEFTIKRDYFITQLNDTLKAISPRTTLPILTGIKIDAKEHEVI
                     LTGSDSEISIEITIPKTVDGEDIVNISETGSVVLPGRFFVDIIKKLPGKDVKLSTNEQ
                     FQTLITSGHSEFNLSGLDPDQYPLLPQVSRDDAIQLSVKVLKNVIAQTNFAVSTSETR
                     PVLTGVNWLIQENELICTATDSHRLAVRKLQLEDVSENKNVIIPGKALAELNKIMSDN
                     EEDIDIFFASNQVLFKVGNVNFISRLLEGHYPDTTRLFPENYEIKLSIDNGEFYHAID
                     RASLLAREGGNNVIKLSTGDDVVELSSTSPEIGTVKEEVDANDVEGGSLKISFNSKYM
                     MDALKAIDNDEVEVEFFGTMKPFILKPKGDDSVTQLILPIRTY"
     gene            complement(22871..24232)
                     /gene="dnaA"
                     /locus_tag="POB55_000075"
     CDS             complement(22871..24232)
                     /gene="dnaA"
                     /locus_tag="POB55_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HDI6499073.1"
                     /translation="MSEKEIWEKVLEIAQEKLSAVSYSTFLKDTELYTIKDGEAIVLS
                     SIPFNANWLNQQYAEIIQAILFDVVGYEVKPHFITTEELANYSNNETATPKETTKPST
                     ETTEDNHVLGREQFNAHNTFDTFVIGPGNRFPHAASLAVAEAPAKAYNPLFIYGGVGL
                     GKTHLMHAIGHHVLDNNPDAKVIYTSSEKFTNEFIKSIRDNEGEAFRERYRNIDVLLI
                     DDIQFIQNKVQTQEEFFYTFNELHQNNKQIVISSDRPPKEIAQLEDRLRSRFEWGLIV
                     DITPPDYETRMAILQKKIEEEKLDIPPEALNYIANQIQSNIRELEGALTRLLAYSQLL
                     GKPITTELTAEALKDIIQAPKSKKITIQDIQKIVGQYYNVRIEDFSAKKRTKSIAYPR
                     QIAMYLSRELTDFSLPKIGEEFGGRDHTTVIHAHEKISKDLKEDPIFKQEVENLEKEI
                     RNV"
     gene            24963..25100
                     /gene="rpmH"
                     /locus_tag="POB55_000076"
     CDS             24963..25100
                     /gene="rpmH"
                     /locus_tag="POB55_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HDI6499074.1"
                     /translation="MVKRTYQPNKRKHSKVHGFRKRMSTKNGRKVLARRRRKGRKVLS
                     A"
     gene            25221..25574
                     /gene="rnpA"
                     /locus_tag="POB55_000077"
     CDS             25221..25574
                     /gene="rnpA"
                     /locus_tag="POB55_000077"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HDI6499075.1"
                     /translation="MLLEKAYRIKKNADFQRIYKKGHSVANRQFVVYTCNNKEIDHFR
                     LGISVSKKLGNAVLRNKIKRAIRENFKVHKSHILAKDIIVIARQPAKDMTTLQIQNSL
                     EHVLKIAKVFNKKIK"
     gene            25713..27092
                     /gene="mnmE"
                     /locus_tag="POB55_000078"
     CDS             25713..27092
                     /gene="mnmE"
                     /locus_tag="POB55_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HDI6499076.1"
                     /translation="MDLDTITSISTPMGEGAIGIVRLSGPQAVEIADKLYKGKHLLND
                     VPSHTINYGHIIDPESKEVVEEVMVSVLRAPKTFTREDIIEINCHGGILTINRVLELT
                     MTYGARMAEPGEFTKRAFLNGRIDLSQAEAVMDFIRSKTDRASKVAMNQIEGRLSDLI
                     KKQRQSILEILAQVEVNIDYPEYDDVEDATTEFLLEQSKEIKQEINRLLDTGAQGKIM
                     REGLSTVIVGKPNVGKSSMLNNLIQDNKAIVTEVAGTTRDVLEEYVNVRGVPLRLVDT
                     AGIRETEDIVEKIGVERSRKALSQADLILFVLNNNEALTQEDYTLYEVVKNEDVIVIV
                     NKMDLEQNIDINEVKDMIGDTPLIQTSMLKQEGIDELEIQIRDLFFGGEVQNQDMTYV
                     SNSRHISLLKQARQTIQDAIDAAESGVPMDMVQIDLTRTWEILGEIIGETASDELIDQ
                     LFSQFCLGK"
     gene            27159..29036
                     /gene="mnmG"
                     /locus_tag="POB55_000079"
     CDS             27159..29036
                     /gene="mnmG"
                     /locus_tag="POB55_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HDI6499077.1"
                     /translation="MVQEYDVIVIGAGHAGVEAGLASARRGAKTLMLTINLDNIAFMP
                     CNPSVGGPAKGIVVREIDALGGQMAKTIDKTHIQMRMLNTGKGPAVRALRAQADKVLY
                     QQEMKRVIEDEENLHIMQGMVDELIIEDNEVKGVRTNIGTEYLSKAVIITTGTFLRGE
                     IILGNMKYSSGPNHQLPSITLSDNLRELGFDIVRFKTGTPPRVNSKTIDYSKTEIQPG
                     DDVGRAFSFETTEYILDQLPCWLTYTNAETHKVIDDNLHLSAMYSGMIKGTGPRYCPS
                     IEDKFVRFNDKPRHQLFLEPEGRNTNEVYVQGLSTSLPEHVQRQMLETIPGLEKADMM
                     RAGYAIEYDAIVPTQLWPTLETKMIKNLYTAGQINGTSGYEEAAGQGLMAGINAAGKV
                     LNTGEKILSRSDAYIGVLIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLRLTDMGY
                     ELGMISEERYARFNEKRQQIDAEIKRLSDIRIKPNEHTQAIIEQHGGSRLKDGILAID
                     LLRRPEMTYDIILEILEEEHQLNADVEEQVEIQTKYEGYINKSLQQVEKVKRMEEKKI
                     PEDLDYSKIDSLATEAREKLSEVKPLNIAQASRISGVNPADISILLIYLEQGKLQRVS
                     D"
     gene            29036..29755
                     /gene="rsmG"
                     /locus_tag="POB55_000080"
     CDS             29036..29755
                     /gene="rsmG"
                     /locus_tag="POB55_000080"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HDI6499078.1"
                     /translation="MTVEWLAEQLKEHNIQLTETQKQQFQTYYRLLVEWNEKMNLTSI
                     TDEHDVYLKHFYDSIAPSFYFDFNQPISICDVGAGAGFPSIPLKIMFPQLKVTIVDSL
                     NKRIQFLNHLASELQLQDVSFIHDRAETFGKGVYRESYDVVTARAVARLSVLSELCLP
                     LVKKGGQFVALKSSKGEEELEEAKFAISVLGGNVTETHTFELPEDAGERQMFIIDKKR
                     QTPKKYPRKPGTPNKTPLLEK"
     gene            29798..30637
                     /gene="noc"
                     /locus_tag="POB55_000081"
     CDS             29798..30637
                     /gene="noc"
                     /locus_tag="POB55_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HDI6499079.1"
                     /translation="MKKPFSKLFGLKNKDDIIGHIEEDRNSNVESIQIERIVPNRYQP
                     RQVFEPNKIKELAESIHEHGLLQPIVVRPIEEDMFEIIAGERRFRAIQSLNLPQADVI
                     IRDMDDEETAVVALIENIQRENLSVVEEAEAYKKLLEIGDTTQSELAKSLGKSQSFIA
                     NKLRLLKLAPKVLLRLREGKITERHARAVLSLSDSEQEALIEQVIAQKLNVKQTEDRV
                     RQKTGPEKVKAQNLRFAQDVTQARDEVGKSIQAIQQTGLHVEHKDKDHEDYYEIKIRI
                     YKR"
     gene            30954..31307
                     /locus_tag="POB55_000082"
     CDS             30954..31307
                     /locus_tag="POB55_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HDI6499080.1"
                     /translation="MFSIGSAILHFVIGGIAVALASIIADKVGGKLGGIIATMPAVFL
                     AAIIALALDHRGTQLVEMSMNLSTGAIVGILSCILTVFLTSLYIKHKGYRKGAIFTVV
                     CWFVISLAIFSIRHL"
     gene            31375..31770
                     /locus_tag="POB55_000083"
     CDS             31375..31770
                     /locus_tag="POB55_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HDI6499081.1"
                     /translation="MKLTLMKFFVGGFAVLLSYIVSVTLPWKEFGGIFATFPAVFLVS
                     MFITGMQYGDKVAVHVSRGAVFGMTGVLVCILVTWMMLHMTHMWLISIVVGFLSWFIS
                     AVCIFEAVEFIAQKRLEKHSWKAGKSNSK"
     gene            32030..32599
                     /locus_tag="POB55_000084"
     CDS             32030..32599
                     /locus_tag="POB55_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI6499082.1"
                     /translation="MNLSKQIKKYRERDGYSQEYLAEKLYVSRQSISNWENDKSLPDI
                     HNLLMMCELFNVTLDDLVKGTIPFVPDIKAQRSLNLWTYVMLIFMTLAAILMGPLVVY
                     WNWTWGVTVAIILGIGFYASMKIEDLKKVHKMDNYDRIVAFMNGKDPSEVQTTKARNT
                     MTNALSIISVIGILSLIIFLSVYLANKFL"
     gene            complement(32717..32917)
                     /locus_tag="POB55_000085"
     CDS             complement(32717..32917)
                     /locus_tag="POB55_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDI6499083.1"
                     /translation="MNNGTVKWFNAEKGFGFIEQENGGDVFVHFSGIASDGYKTLEEG
                     QKVTFEITEGQRGDQAVNVQTV"
     gene            complement(33310..33501)
                     /gene="vraH"
                     /locus_tag="POB55_000086"
     CDS             complement(33310..33501)
                     /gene="vraH"
                     /locus_tag="POB55_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide resistance ABC transporter activity
                     modulator VraH"
                     /protein_id="HDI6499084.1"
                     /translation="MKFRELVKQSYEDLKNLTVTWYNVIALAMIVIVLSVVTTPIIGI
                     PAGLLGGAYYLKRREEKGK"
     gene            complement(33594..35474)
                     /gene="vraE"
                     /locus_tag="POB55_000087"
     CDS             complement(33594..35474)
                     /gene="vraE"
                     /locus_tag="POB55_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide resistance ABC transporter permease
                     subunit VraE"
                     /protein_id="HDI6499085.1"
                     /translation="MTFNHIVFKNLRQNLKHYAMYLFSLFFSIVLYFSFTTLQFTKGV
                     NNDDSMAIIKKGALVGSIFLFIIIVIFLMYANHLFVKRRTREFALFQLIGLTRQNILK
                     MLALEQMIVFLITGVVGVLCGIAGAQLLLSIVSKLMSLSINLSIHFEPMALVLTIFML
                     IIAYVLILFQSALFLKRRSILSMMKDSIKTDVTTAKVTVIEVISGVLGIAMIALGYYM
                     ATEMFGTFKALTMAMTSPFIILFLTVVGAYLFFRSSVSLIFKTLKKSKNGRVSITDVV
                     FTSSIMYRMKKNAMSLTIIAIISAVTVTVLCFAALSKSNTDQTLTSMAPNEFNVVATQ
                     DAKQFETKLSQQQITFSKNAYETITVDNVKDQVITLENGSDSGRTNSILSANNKLTGN
                     NAIITNTKSLPNIINIHLNKDLVVKGTKNETFRVTQEDKGKVYPLNLSFNSPVIEVSP
                     EKYQQLKTQNNVHTFYGYDIKQTSQKEKAQTIAKQFGDKVITYDEMKKEVDATNGILI
                     FVTSFLGLAFLVAAGCIIYIKQMDETEDELSNFRILKRIGFTHTDMLKGLLLKITFNF
                     GLPLLIAILHAVFAAIAFMKLMGNISFMPVIVVIVVYTLIYITFALIAFVHSNKLIKK
                     TI"
     gene            complement(35464..36222)
                     /gene="vraD"
                     /locus_tag="POB55_000088"
     CDS             complement(35464..36222)
                     /gene="vraD"
                     /locus_tag="POB55_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide resistance ABC transporter ATP-binding
                     subunit VraD"
                     /protein_id="HDI6499086.1"
                     /translation="MTILSVQHVSKTYGKKHTFQALKDINFDIQKGEFVAIMGPSGSG
                     KTTLLNVLSSIDQISSGSVIANGQELNKLNQKALAKFRKESLGFIFQDYSILPTLTVK
                     ENIMLPLSVQKMSKATMEENYKAITTALGIYDLGNKYPSELSGGQQQRTAAARAFVHK
                     PQIIFADEPTGALDSKSANDLLQRLEEMNKSFDTTIVMVTHDPVAASFAERVIMLKDG
                     QIHTQLYQEGRSKQAFYEDIVHLQSVLGGVSNDI"
     gene            36505..37017
                     /locus_tag="POB55_000089"
     CDS             36505..37017
                     /locus_tag="POB55_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HDI6499087.1"
                     /translation="MAKGNQGIKWSSLIMGVLLLMLAVVIFTFPIENFYAITWLIGLF
                     VLINGVIQIIYRRKAKALVGGNQNWILFMGIVDILFGLIVIFNVGASSAFFIYMFAFW
                     FIFSSIAGLFTFTGSGSLKLISVIFNLLGIVFGVILLFNPLMGIVFISTMIAIAFVFV
                     GVIYVVDALA"
     gene            complement(37151..37945)
                     /locus_tag="POB55_000090"
     CDS             complement(37151..37945)
                     /locus_tag="POB55_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HDI6499088.1"
                     /translation="MNIAKLENYLQIDSSRYNRPSIEALNYYATRFMLTVPFENIDVQ
                     NSKPISINIDALFNKIVHDKRGGFCYELNTFFKAYLQQKGFNPALMSATIHTPGGGRS
                     LNGSHASLVVSINDVFYVTDVGFGDLPIHAIPITSSEHTQPITDISGTFRAIFNNEDK
                     DIFYVQKFENDHWHTKYEAEFKPKQIEDFNSNIEYNQTNPDSIFVQHLLITMPQSFGR
                     ATMSENHLTLTRNGSSEKLAVTKNNYKHFLEKYFGLDITINRIEKQ"
     gene            complement(38188..39204)
                     /locus_tag="POB55_000091"
     CDS             complement(38188..39204)
                     /locus_tag="POB55_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HoxN/HupN/NixA family nickel/cobalt transporter"
                     /protein_id="HDI6499089.1"
                     /translation="MTVFKNERLSWLPYIAIVILLHVIGFSFLWIAGKDHHILFGMGI
                     LAYTLGLRHAFDADHIAAIDNTVRKLLQQRKDPSGVGFYFSIGHSSVVFLMAVFLGVS
                     VKWAKDELPHFQDIGGTIGTLVSGFFLVLIGVLNLIILISLINLFAKLRREHIEEAEV
                     DALLESRGLVSRFVGPYFKLITRSWHVLPLGFLFGLGFDTASEIALLALSSGASQQAI
                     SFIGILSLPILFASGMSLLDTLDGVVMKYAYNWAFFNPIRKIYYNITITAISVMAALV
                     IGMIELLQILADKLDLHGAFWAFIGSIEFDYLGYILVALFLITWLISSLIWKFGRIEH
                     KWSK"
     gene            complement(39370..39945)
                     /locus_tag="POB55_000092"
     CDS             complement(39370..39945)
                     /locus_tag="POB55_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDI6499090.1"
                     /translation="MTLPKIGKPATRALNSQGIYTLEAVSQYTKSSLMEMHGVGPKAI
                     SILEQALFQHQLHFKTEVQSSLPFKLTGDVSCNHAPKRQQMIDFIVATAALDIELLRS
                     LVTTEFIWSVPGRFDIYGPQILIQELSNHYNQVASLNIHSSITHGCLGSMHGIEILKT
                     GKEIHFAHFFEFENHKKDAKLSKVTSYIVVG"
     gene            40167..41075
                     /gene="rarD"
                     /locus_tag="POB55_000093"
     CDS             40167..41075
                     /gene="rarD"
                     /locus_tag="POB55_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HDI6499091.1"
                     /translation="MNTEYKKGIFLALSAYILWGILPIYWQFVDEIGAFEILAFRIIF
                     SAIFMIFILAVGQKQRNAFQRDMNQLLGKPIQLLAIVVAGYVITLNWGTFIWAVTNGH
                     VLQTSLGYYINPLVSILLALIFLKERFNKFEWLAILFAFIGVLYMTLKIGEFPIVSII
                     LALSFGTYGLLKKVVHIDAISSITIECIVTAPAGLIYVIYLWQQHQMSFGLNMSSFWL
                     LFSGAITAIPLILFSAGAKRIPLSLIGFIQYVGPTIMFVLGIFVFKEPFNIDQLITFI
                     FIWTGIVLYSLSQYIKLKKHPVAKTL"
     gene            complement(41488..42906)
                     /locus_tag="POB55_000094"
     CDS             complement(41488..42906)
                     /locus_tag="POB55_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HDI6499092.1"
                     /translation="MENTVKYRKFILPIVVGLLIWALTPFKPDAVDPTAWYMFAIFVA
                     TIIACITQPMPIGAVSIIGFTIMVLVGIVDMKTAVAGFGNNSIWLIAMAFFISRGFVK
                     TGLGRRIALHFVKLFGKKTLGLAYSIVGVDLILAPATPSNTARAGGIMFPIIKSLSES
                     FGSKPKDGSARKMGAFLVFTEFQGNLITAAMFLTAMAGNPLAQNLASSTSNVHITWMN
                     WFLAALVPGLISLIVVPFIIYKIYPPTVKETPNAKSWAENELATMGKIALAEKFMIGI
                     FVVALTLWIVGSFIHIDATLTAFIALALLLLTGVLTWQDILNETGAWNTLVWFSVLVL
                     MADQLNKLGFIPWLSKSIATSLGGLSWPIVLVILILFYFYSHYLFASSTAHISAMYAA
                     LLGVAIAAGAPPLFSALMLGFFGNLLASTTHYSSGPAPILFSSGYVTQKRWWTMNLIL
                     GFVYFIIWIGLGSLWMKVIGIF"
BASE COUNT        13923 a         7537 c         7102 g        14582 t
ORIGIN      
        1 ttttccaata taaacttata tattactatt ttttattagt ttctcacggc ggattgtgac
       61 aatgatattt aacacaatga tgaggtctct attccaataa taaaattagg ataatggcaa
      121 cttctagttg cacgtcattg ctcagaaaat aaatatcatt attcgttatt cgttacttta
      181 acaattgatg ttatggagga ctggtggcat atcacaacga agaaatatgt tgacagtagc
      241 atatgtttgt acgtctaata aagattttat tgtctacgtt tatctttcat atccaaccac
      301 taagttactt gaggttcata cattttaaag aaaatggacg aacagataaa tcaatatgat
      361 tatcattcgt ccatatatct tttattaata taatattaaa cgcaaaaaaa tgcctatcct
      421 cttggatagg catttgtacg gtctcgacgg gaatcgaacc cgcgatctcc tgcgtgacag
      481 gcaggcgtgt taaccgctac actacgagac ctaaaaaatg gtgactccta cgggactcga
      541 acccgtgtta ccgccgtgaa agggcggtgt cttaaccgct tgaccaagga gccgttctta
      601 agcacaagat agattatagc acagccaatc ttgccatgac aagatatttt ttacataatt
      661 ttgctattat tttttgaaga atgttataag tgaaaaattg cattttattg gcagtctatt
      721 tattataata attacgctat ttttgaaatt tttctatttt aggtgctttc atttacactt
      781 acgtctactc tattttaata gctattttcg acatcagact tcttacattc aagtatttca
      841 tcttaatttt cgttaaatat catctaatct tctaaattat gactagcaaa tatcgccttt
      901 acgttagtat tttttatcta aataatcgaa tcgatacaga agcaagcata acaaaagcct
      961 cctaccaata aagatagaag gctcatgtat ttataatcat tgtgtatgac ttatataaag
     1021 ttctaccaca attcttttaa taggtttgtt tgttctctat ctggaccaac tgagaagata
     1081 gaaatttgta cattacataa ttctgaaata cgctctaaat atttacgtgc attttcaggt
     1141 aattcttcta aagtacgcac atttgttacg tcttctgtcc aacctggtaa ctcttcaaag
     1201 attggtttac aacgttttaa ttgatctaag tttgctgggt actcagtaat ttctttaccg
     1261 tctaattcat aagctgtaca gattttcact gtgtctaggc ctgttaaaac atcgattgag
     1321 ttaatagata aatctgtaat accacttaca cgacgagagt gacgtaatac aactgaatca
     1381 aaccaaccta cacgacgtgg acgtcctgtt gttgtaccgt attcacgacc aacctctcta
     1441 atatgatgtc catcttcatc gaataattca gtagggaatg gaccatcacc aacacgtgat
     1501 gtataagctt tacatacacc aattaccttt gaaacgaatg taggacctac acctgtacca
     1561 acagtaacgt tacctgcaat tggattactt gatgtaacga atggatatgt accatggtcg
     1621 atatctaaca ttacaccttg cgcaccttcg aaaagtacct tttcatctgc tacaaatgca
     1681 tcgtctaaga tttttgatgt gtctgttaca aattctttta aacgttgacc tgcagcatag
     1741 tattcttcaa agatatcatc aaatgatgga catgtttcgt taaacatacc tttgaaatat
     1801 gcttgtttat attcaatgtt tgattttaat aatctttcga atgtttcttt ttcaagtaaa
     1861 tctgccatac gaataccgat acgttgaact ttgtctacat atgctggacc gatacctttt
     1921 ttagttgtac caatcttatt gtcaccgcgt aaacgttctt catattcatc ttgtgctaag
     1981 tgatatggta aaatcacttg cgcacgatta gatatacgta aattacttgt aggaatgcca
     2041 cgttcattta atccgtctaa ttctttcaat agtgcaactg gatcaacaac gacaccgtta
     2101 ccgattaccg ctaatttgtc tttgtaaaag ataccagatg gtactaaatg taatttatat
     2161 gtttctccgc caaattgaat ggtatggcct gcattattac cacctgaaaa acgcgcgata
     2221 acatctgact gttctgccaa gaaatccgtt atttttcctt ttccttcgtc tccccattgt
     2281 gtcccaacta ctacgattga tgacatgtga gcacctccaa gtttttctca tttaaccatt
     2341 tgatattcta ccaatatcga gaccataatg caaataaaaa tcgaacattg attttcagcc
     2401 tttttaaaag aaaaatcatt ttaacaaatc gtcttcaacg tcctaaaaac cctttaacag
     2461 aggcgttgac agtactaatc catcactttc aatacacaaa cttaacatat atcaacccgt
     2521 tcagaacaaa ataaaaaccg tacaatttta tcatcgcaat gattattgta cggaaaaact
     2581 ttttttacat catatcagca tgtgcataat cgatatctgt aaatttatta tattgtttca
     2641 taaagtgtaa cttaactgtg cctgttggac cgttacgttg cttagcaatg atgatttcta
     2701 tttcaccgtt ttcatcattc gtttgtggct caaaaccacc atcatcgtca tcatcttcat
     2761 cgccgccacg gttatagtaa tcatcacggt ataagaacgc aacgatatca gcatcttgct
     2821 caatcgaacc agattcacga atatcactca tcattggacg tttatcttgt cgttgttcaa
     2881 caccacgaga taactgactt aatgcgataa ctggacattc taattcacgg gctaatgctt
     2941 ttaatgtacg agagatttca gaaacttcct gttgtctgtt atcggacgca cgtgaaccac
     3001 taccttgaat caactgtaag tagtcaatca caatcatgtc taagccatgt tcttgcttta
     3061 atcgacgaca tttagaacgt aaatcattaa ttcgaatacc cggtgtatca tcaataaaaa
     3121 tcttcgtacg tgataattta cctaccgcta tagtaaaacg actccaatct tcctcagtca
     3181 tagtacccgt tcttaagcgg tttgagtcaa catttccaga actacaaatc atacgtgtgg
     3241 ctaactgatc agcacccatc tctagcgaga aaataccaac tgtatacata tcttcatgcg
     3301 ttgcaacttt ttgtgcaata ttaagtgcga acgcagtctt acctacagat ggacgcgctg
     3361 caaggataat taaatcattt cggttgaacc ctgctgtcat ttggtctaaa tctcgatatc
     3421 ctgtaggtat acctggtgtt tgaccactat tttgatcaag ctcttcagct gtttcataca
     3481 cttgtcctaa gacgtctcga atgtctttaa agccatcgct ttcacgagaa gatgatagct
     3541 ctaaaattcg acgttctgca tcacttaaaa tcgcatctag ttcaagttca tcattatatc
     3601 catcattggc aatactatct gcagtttgaa tcaatctacg ttttaatgca tgcttagaaa
     3661 cgatatcagt ataatactga acatttcgcg tcgttggtac atttgtagat aactctgcaa
     3721 gatattgcgg gccacccgct tcattcaacg taccttccgt cgataattga tccatcaatg
     3781 ttacaacatc aatttcttta ttatcttcat ttaagtgcat cattgcacgg aaaatatgtt
     3841 gatgggcacc cctataaaac gactcaggaa gcaaaacttc ctgagtagta ttaatcaatt
     3901 ctggatctat aataattgaa cctaagacag actgttcagc ttcattgtta tgcggcattt
     3961 gattttgctc atacattcta tccatgaatg gttacacctc ttatttcaat ccaactttat
     4021 tgttcaactg tgtgtacgcg aattgtacct tcaacttctt tatctaattt aacaggtaca
     4081 ttcgtatatc ctagggaatg aattccattt ggtaaatcca ttttacgttt atcaatttta
     4141 atatcatgtt gtgcttttag tgcttcggca atttgttttg tacttactga cccaaacaat
     4201 ttaccacctt caccagtttt tgctgatact tcaacttcaa tgtttgataa cgtttctttt
     4261 aatgctttag catcttcaat ttcttgttgg cgttcttgtt ttgcacgttt tttctgtaac
     4321 tctaattgtt taaggttacc tggtgttgct tctacagcat aattcttttt caataagaag
     4381 ttatttgcat aacctactgg tacttcttta acttcacctt ttttaccttt acctttaaca
     4441 tcttgtgtaa aaattacttt catgcatctt cactcctact taattgttct gtaattgctt
     4501 gttgtaattg tgctatcgcc tcttcgactg tcacaccttt aagttgtgtt gccgcattgg
     4561 ttaaatgtcc accgccacca agtgcttcca ttgttaactg gacatttact gaaccgagtg
     4621 aacgcgcaga tataccaatc agattatctt cacgtctcgc aacaacatat gatgcttcaa
     4681 taccttctaa acttaacagt tcatctgctg cttgtgcaac tgttactgga tgataaattt
     4741 tatcgtctga accatgcgca attgctatgc cattatcttc aacttttaca gttcgaatta
     4801 attcagatcg attaatgtaa gtatccacat catcttttaa gaaatgttgc gttaaaatcg
     4861 tatctgcacc atgtgcacgt aaataactcg ctgcatcgaa tgttcttgat cctgttcgta
     4921 atgtaaagtt tcttgtatct acaataatac ctgcatacat cactgttgat tcaagacgtg
     4981 ttaaacgttg ttctgttggt tgatattcca gtaactctgt taccaattca gctgtcgaac
     5041 ttgcgtatgg ttccatatat atcaacaatg gattagagat gaagctttca ccacgtctat
     5101 gatgatcgat aacaacttta cggtttgctt tatttaagac attttcatct aaaaccagtt
     5161 ccggtttatg cgtatcaaca atcactacgg ttgtcttaga tgtcatcata tcccaagcat
     5221 catctgatgt aataaatcgc tctcttaact ctggcttttt atcaatttcg ttcatcacgc
     5281 gtcgtaatgt tggatcaatg tcagtctcat ttaatacgat gtatgcttct aaattattca
     5341 tcattgcaaa tctagacaca ccgattgctg caccaattgc atctaagtca ggacgtttat
     5401 gtcccatgat aatgactttg tcaccctctg caaggatatc ttttaacgca tgtgagataa
     5461 cacgcgctct tacacgagta cgtttctcca tcgggtcagt cttaccgcca tagaagcgca
     5521 cattaccatt aatactttta attgcaactt ggtcgccacc gcgtcctaat gctaagtcta
     5581 ggcctgattg tgataattca cctaaatcga ttaaattttc agtaccttca ccaacaccaa
     5641 tacttaatgt taattgggca cgataaccaa cacttttttc acgtaattga ctcaagatat
     5701 caaatttaga ttcttctaag tcagctaata ttttttgatt taaataggct acgaattgat
     5761 cggaactata ccttttaaag aatatattat actcagttgc ccatcgacta ataacacgcg
     5821 ttaccattga attgatttcc gaacgctgcg tatcattcat attttgagta atctcatcgt
     5881 agttatctaa aaataatgtc gcaatgattg gtttagaatt ttcatatagt tcatttgttt
     5941 gtacttgttc agttatatca aagaaataga ggcagtgatc attctcagaa taacgtactt
     6001 ggaaatgata ctgattatat tctatttcaa cggatttcac tctatctaat tgctttaaaa
     6061 tgtttggaaa tacttcattt acagattcag aaatgacatt tgcttccata tgatctgtca
     6121 taaattggtt aacccattcg atgtgatcat tttcatctaa aacaatgata ccaattggta
     6181 aatgtttgat tgctttatta tttgttgttg aaatttgagc actcaaacca tctacataac
     6241 tatccatttt cattaaagct tgtctgaata aaacgatgct aacaataatc atcacgacaa
     6301 gaacgataga tgcaattagt gctataagac tattaaagat aaaccataca cccattaaaa
     6361 caattgctgt gatgatcatg atgacaaatg gtattagtaa agctttctta gtggactgcc
     6421 gattcattat tccacctcta ttcacttttt agaattattt ttcatgattc gcttcaaatt
     6481 caaacttaaa tcgataacac caagtagtcc tacaatatgt gtcgtaggtg tcagtattgt
     6541 accgataatc aatagtaaaa tcgttactgc attcggcaaa cctttcgctt taccaaagaa
     6601 atgaataaca cttaaacctt gaatatacat tactaatgat aacacaagtt ggaagtttaa
     6661 aagaatgctc tggaacacac tcggttgacc tgtaaataat aaacatatga taacaataat
     6721 gtatatccat aataaaatac cgctcatttg ccacgcgaaa agtggcttaa atacaggtgt
     6781 agcgatttta aattttcgta aaatcggaaa tgtaacgatt aagttaatta agacgattaa
     6841 aaatgtaatg ataatgatga aacctggtaa ttgaacggtc gcttgtctaa acccttcttc
     6901 taatatttgg gtcatattcg catcggcacc gctcatcgta atcgcttcat gtaatgtttg
     6961 cttgaaaggt tttactatgc tcgctgatgg tggaattctt ccgaatgttt gtagtaacat
     7021 aaaagcgatt aatgaaatta agctcatcgc tactgttgtt acgtataata ttctttcttt
     7081 agacgttctt tctttgagca attgaccaat aattaaactt gcaattaaga ctaatatgat
     7141 ggcacttaaa acgaaagtat tacctaaaac agttgttata attactgtaa taagtgcact
     7201 aatcccgaaa gattgtattg atttattcca taaaacgata cctggtatgg ttgcaaacaa
     7261 tgcaaatatt agtcctaaac caggcactag atataaagct aaagcgacaa ataccaacgc
     7321 aatcgttgca attattgttg ctttaggttg tatttttgaa aacacataag ccactcccat
     7381 atttttaact atagctatta ttttaacctc tttaatggaa tttaacaatt tatagattgt
     7441 atgcttctat ttcatttaat tgaataataa ctttcatgtt ttataagtaa ttaacatact
     7501 catttgaatc gcttttgtgt gctttcattt tcaacatgat tatttaatcc cactacatag
     7561 caatcaagct tgatttagat ttacaattca ttaccactct catgtactct agatgttttt
     7621 gaatatgata gctgtgattt agtggcttca ttctttgaaa atatatatat tattacttac
     7681 gcttaaaatg ctttaaattt aagaaatgat ataagttagg tgcccaggta ctaaagttta
     7741 gtaggaatcc atcatgccca acattatcag gcacgaagaa atgacgatga tatttaaaac
     7801 gttcacctaa tgcacgaact tgatcatccg gatatagcaa atcatctatg aaccccatcg
     7861 ttaacacttt tgtttctaaa tttttaaaaa catgcgttac gtctgtgcga cctcggtcaa
     7921 tgttgtgact atccaataca tctagcagtg tcagataaca attcaaatca aaatgttctt
     7981 taaatttatt accttgatgt tgttggtatg cgactacttc atccggcgtg aaacgttcat
     8041 cataactttt tgatgatcga tatgtcaaaa aacctaattg gcgtgcaata cttagacctt
     8101 ccttaccacc aagatgaatg gcttgccttg caatttcatt gaaagctcta ctataagatg
     8161 atgttcggct tgttgcagca aggataatgg ctttatctac ttcaaactgt tgattgtaaa
     8221 gtagttccat tgcttgcatt cctccaaggc ttccccctat taaaatatta atcttgtcat
     8281 aaccaagggc ttgtatacct cgttcattcg ctctgactat atctcttaat gttaattttt
     8341 taggaaaatg agggtcgttt aaaggtgaac ttgaaccgaa aggactacca ataacatcaa
     8401 atgttaaaaa ttgataatcg tgaatgggta tatatccccc atcaataatt tctcgccacc
     8461 aacccggata atcatctgtt ccatatgtta aatgattgcc agttaatgca tgacaaacta
     8521 caactaatgg ttgtccatga taaccgacat gctcatatct caaacgcaag ttatctatga
     8581 cttccccaga ttctgtaata aatttcccta aatttaaagt atctactgtg taatttgtca
     8641 ttgttttttc ctccttaaac aaaaaaaact tctcacccta ttgaaaagta agaagtcttt
     8701 atacttatca ttcgagtaac tcgttggttt tagcaccgtg ctataaagtc ggttgctgaa
     8761 gtatcacagg gccaagtccc tcaacttctc acaataagaa atatgaaatt gttatgtgtt
     8821 agttgagatt cagtgatgaa ttactttatc atttaaaatg ttgttatcat tgtcatgcgt
     8881 tagccaaatc gcttacgtat acacgattcc caatcttaac atagacgatt tgtatatcag
     8941 aattttctga ttactaacag tttacctaag tttaaatatc tgttcaatga ttttcagtta
     9001 tttttaaaag aaaaatcgta atgctgccat gataacaatc ccactaataa ttgtaatagt
     9061 taaactacgc gtgattatag ataaaataac cgtcggaatg agcgcgataa tgtaagggat
     9121 gtttaatgta tacccctcac catgaggcgt ctgttgaata atgctgtcta tgacaagtgc
     9181 cgtaaatagt gtgattggga taaatgatag ccatcgaacc acgacatcag gcaattgcac
     9241 ttttgaaatc atgataaaag gtataattcg aattaatagc gttacgatac cacacaataa
     9301 aataagtatt aacatgttca tatgagttat catcgttcca tcatcactcc taacgctgct
     9361 gaaattgtgg ctgcaattaa tattgctaga tatgaaggca taaacatact tagcgataac
     9421 atcattacta tgacggcaat aatgagtact atgtaaattc ttaatcgcga tttagtaatt
     9481 gattcaaatt gcgcaatggc caaaaagata aacatagccg tgatagcaaa atctaaccct
     9541 aatgcttgtg gattcgaaat atactcgcca aacaatgcac ctgctacaca tgaaattgcc
     9601 caaaataaat atgctgtgat attaagacca tgcatccaac gatcattgat agcttctcct
     9661 tttaaataag gtgtaatggc gacgccaaac gtttcgtcag ttactaatga acctaatcca
     9721 acacggttcc aaaacccata tgtcttgaag tttggtgcaa gcgacatact taaaaggaac
     9781 attcttgaat ttacgataaa tacagttagt acaatcgctg atatcggtgt acctgctata
     9841 aacaacgcgc acataataaa ttgcgcagca ccggcatata taacaagaca taacaagaca
     9901 atttctaaaa tactaaagtt ttgagacgaa gccacaatac caaatgaaat accaacaccg
     9961 gcataaccca ataatgttgg gacacactct cgcacgcctt gtctaaaact taaatgtgtt
    10021 gtcatctcaa ctacctcctt tgccacttta tatttatata attacgaaaa aaataatata
    10081 cctagtctaa tacagttcta tatcacaagc aataacgtta gacactggtt tgtctgaagt
    10141 tagacatacc ggagtcttta atcagcttca tattaacttt ttattttttt agaattttca
    10201 attttttatt taagactacc tccatatttt ctatggattg tagttgtttt taaatactaa
    10261 ttttataaat ttttatatct aatgatgagt ctgggatatt gattcatgta ccactccctt
    10321 ataatcatca tcccctcccc ctaccctact ccatcgatat tactcatact acatcaacga
    10381 aatcagtatt ttatcgctta atttcctata atagtgatgc tcaaaattgt tacgttttag
    10441 attgttttag ttcatcataa ttatcccgta ttgttgctat aatgaaatgc gttcacccca
    10501 ttaaacaaca aacttaattt attgttgtta tgtgcattgg ctcactacta tacttttaca
    10561 gcacaaaaaa agtggcgaca gcttcgtcac cactttttaa aatattattt aaagtatctt
    10621 gcccttgctt taagtatacg tagatatata ctttttaaag cttgtagcta aagcctttat
    10681 ttaactggtt ttgaaatttg tgttttacca cccataaatg gtactaatgc ttctggaatt
    10741 gttactgttc catcttcatt ttggtaattt tcaacaatag cagcaaatgt acgtccaact
    10801 gctaaaccac taccatttaa tgtatgtgct aattctggtt tagctgcttt gtcacgcttg
    10861 aagcggatgt tagcacgacg cgcttggaaa tccgtacagt ttgagcatga actaatttct
    10921 ttataatcat tgtagcttgg taaccaaact tctaaatcat atgttttgct tgcactaaat
    10981 ccaatatcac ctgtacataa aataacacga cgatatggta aacctaactc ttctagaatt
    11041 gcttctgcgt ttgttgtcat ttcttctaaa gcattccatg aatcttcagg ttgttcaaaa
    11101 cgtaccattt ccactttatc gaattgatgt aaacgaatta atcctcttgt atctctacct
    11161 gctgatcctg cttcactacg gaaacatgca gattgaccag tgaatttttc aggaagtaca
    11221 cctggttgaa taatttcatt acggtagaaa ttcgttaatg gtacttcagc agttggaatt
    11281 gtatataatc cttctttttc tactttaaat aaatcttctt caaatttagg taattgacct
    11341 gtaccataca ttgtatctgc gttcacaagc tgtggtacca tcatttctgt ataaccatgt
    11401 tgtgttgtat gttttgtaat catatagttc attaaagcac gctctaattg cgcaccttca
    11461 tttgttaaat atacaaaacg cgcacctgaa acttttgctg cacgatcaaa atcagccatt
    11521 ttcaattctt ctacaatatc ccaatgtgct ttgggttcaa atgaaaactc acgtggtgta
    11581 ccccactttt taacttcaac gttatcttca tcagattcac cttgaggtac atcatcactt
    11641 attaaatttg gaatacgaca aaggatacct gtcattttat tatcaatttc atttaattga
    11701 ctatcttttt ctttaatatc gtcacctaat gtgcgcattt cagcaatcac atcatcagca
    11761 ttttctttat tacgtttttt taatgcgatt tcttcgctta ctttattacg acgtgctttc
    11821 atttcttctg ttgcactaat taatttacgt cgttgctcat ccaattctaa aatttcatct
    11881 acaacttttg aatcatctcc acgtaattca attttgctct taactgtgac aggctcattt
    11941 ctgaataatc taatgtctaa cattaacctt catcctttcc caaataatta tcatttatta
    12001 tggaatgacg tacgtcttta ttttttagaa aataaaaaaa gaccacatcc ctacaaggga
    12061 cgtggtctac gcgttgccac cctatttaac aatttaagtt ataaagatac actaaaccta
    12121 aattgcactt cactaaaata acggttatca ccgattgttc ttttaaatta agtaggtaga
    12181 ttcatatata tgttgattct tgttcacact aaccacaagc tctctgatat cgaacactat
    12241 atattacttg tcctacgaac aatgtcttat taagttattt ttaatatagc aaactatatt
    12301 tgctttttca agtaacgatt tcaaacatcg ctcatgtcga tttagtgaca tgcagtcgtt
    12361 tgataaattg attgctttaa atactgtgca accgcttcaa tatctttatg aaattgacgg
    12421 tcatgtgtaa tggatggcac gatacttcga aactcctcat atttacgacg tgtttttggt
    12481 gataaacctt caacaccttt taactctgct gcttgtaatg caataacaca ttcgattgcc
    12541 agcacacgtc ttgcattttc aataatttga taaccatgtc tagcagctgt agttcccata
    12601 gatacgtgat cttcttggtt cgcagatgaa gtgatagaat caacactcgc tggatgcgct
    12661 aaagttttat tttcagaaac gagacttgca gcagcatatt gcataatcat cgcgccactt
    12721 tgcaatcctg gctctggact aagaaatgct ggtaaatcac catttaattg aggatttact
    12781 agtcgctcta aacgacgttc cgatacgttt gctaattcac ttacacctaa tttaagatga
    12841 tctaatgcaa aagcaatagg ttgtccatgg aagttaccac ctgaaataac aaacgtttca
    12901 tttgcttcct caaatataag tggattatca ttagccgcat tcatttcaaa ttctaattgc
    12961 tgtttaacat aattgaatac ttgaaaactc gcgccatgga tttgtggtat acaacgcaac
    13021 gtatatgcat cttgtacacg tatttctgct tgtcgcgtcg ttaatgttga tccttctaac
    13081 caatcacgca tacgcgctgc cacattaatc tgttcttgaa aattacgaac agcgtgcaca
    13141 tcatgtctat atgcatctat aatgccatta agagactgat gcgttaatgc agcaatccat
    13201 tcagattggt aacctaaatc ttctgattct atataactaa tgacaccttg agctgtcata
    13261 gcttgcgtac cattaatcaa tgctaaacct tctttagcct gaaggttcaa aggttgtcta
    13321 tttaattctc ttaatacatc gtcactatcc ttttcttccc ctctgtacaa tactttccct
    13381 tcaccaatta atgctaatgc taaatgtgat aatggcgcta aatctcctga tgcaccgaga
    13441 gagccttgct gtgggattat cggtataata cgttcattta taaaaaattg taattgtctc
    13501 actaattcta aagtggcacc tgaatgacct tttaataatg tattcaatcg taaaatcatc
    13561 atgactaatg ctacttcttt tgaaaatggc tcacctggtc cacaggcatg tgagcgtatc
    13621 agattcactt gtaattcatt atattgcgtc gggtctatac gtacatcact aaataaccca
    13681 aaacctgtag taataccgta aaccgtttcc tcattttcaa taatacgttc aactaccgct
    13741 ctactttttt tgacacgttc taacgcatca tcaataattt caatctttga ttgttgttgt
    13801 aaaaatgatt taatatcctc aattgttagt gtttcaccat ctaaatataa agtcatatat
    13861 gttaccccct tgtttatatt aagtaaccca tccttcttga agtatacgtt ttcattttta
    13921 ttgaaacaat ggttttacgt acatttataa cctattatca gagcactatt gtagtgcgtt
    13981 aaaggatatt aagattgttg taagcatatt taataattta tctattgacg aattgcatat
    14041 acaggtatag tattttctat tgtatttaac gacaaataat aatgaattca gaaatttata
    14101 atacattttg ttaaaagtta ctatatattt ttaaaattga ataaattcgg aaaaggcttt
    14161 tacatgggag gttatatcac tatggaaacg ttaaattcta ttaacattcc taagcgtaaa
    14221 gaagattcac ataaaggtga ttatggcaaa attttattaa ttggtggatc tgctaactta
    14281 ggtggtgcca ttatgttagc ggctcgtgca tgtgtattta gcggtagtgg tttaatcact
    14341 gtagctacac atccaacaaa tcattcagca ttacattctc gttgcccaga agcgatggtt
    14401 attgatatta atgatacgaa aatgttgacg aaaatgattg aaatgactga cagtatacta
    14461 attggtccag gtcttggcgt tgatttcaaa ggaaataatg ccattacatt cctactacaa
    14521 aatatacaac cgcatcaaaa tttaatcgta gacggcgatg cgattacaat ctttagtaaa
    14581 ctgaaaccgc aattacctac atgtcgtgtg atctttacac cacacctcaa agaatgggaa
    14641 cgattaagtg gtattcctat tgaggaacag acatatgagc gtaatcgtga agcagttgat
    14701 cgtttaggtg caactgttgt acttaaaaaa catggtactg aaattttctt taaagatgaa
    14761 gactttaaat tgacaatcgg tagcccagca atggcgactg gtggtatggg cgatacactt
    14821 gctggtatga ttacaagctt tgtcggtcaa tttgataact taaaagaagc ggttatgagt
    14881 gccacatata cacatagttt tattggcgaa aaccttgcaa aagatatgta tgtggtgcca
    14941 ccatcaagac ttatcaatga aataccttac gcaatgaaac aattagaaag ttagtcatta
    15001 ctaatcattg aatatagtaa agcattactt tctagcataa aaataagact cccctacata
    15061 tagggaagtc ttatttttta ttattcttca tctgatgatt gttgtatatc ttcttcaaca
    15121 cgatccatga aatcttgtct tacttcaata cgtccatctt catcattttc ttctgaatca
    15181 atcacttcag tatgaattgc atttcctggt gtttcatcat ttacaaccgc ttcacgttgt
    15241 tgttcagtac catcttcaga tacagttgaa gtagattgct catcttcatt cgtttcatct
    15301 tctgcatctt cttttacttt agcaaccgtt gaaacaaatt gatcatcacc taagcgaatt
    15361 aagcgaacac cttgtgctgc acgaccattt tgagaaatat ctgcaacatc tagtcgaata
    15421 atgacacctg cattagtaac aatcattaaa tcttcttcac cagttactgt agtgatacat
    15481 acaacattac cattacgctc agtaatcgta gctgttttaa tacctttacc accacgattt
    15541 gataaacgat agtcattaac tggcgtacgt ttaccataac cattttcagt aactactaat
    15601 acttcatcaa cactgtttgc atgagctaca tcaagcccta caacttcgtc accttcacga
    15661 agtgtaatac ctttcacacc cgttgctgta cggcctaaag gacgtaatgt tgattcaggg
    15721 aatcgaatta atgatgcatg tgatgtacca atcaagatat cttcttgacc acttgttaag
    15781 cgaactgcaa ttaactcatc atcttctctg aacgaaatcg caatcttacc atttctattt
    15841 attcttgaga agttacttaa tgctgaacgt ttaacgacac cacgtttagt tgcaaacact
    15901 aagaagttgt cttcactttc aaggtcttta acagcaatca ttgtactaat gacttcatca
    15961 ttttcaagtt caatagcatt cactacagga atacctttag actgtcttga taactcaggc
    16021 acttcataac ctttaagttt gtatacacga cctttgttag taaagaacaa tacatggtca
    16081 tgtgtactta aagttaccaa ttgactgaca aaatcttctt ccaatgtatt cataccttga
    16141 acaccacgac caccacggtt ttgagcacga tatgtagata ccggcaaacg tttaatgtag
    16201 ttattatggc ttagtgtaat tactatttgt tcttctggaa ttaagtcttc gtcctctaag
    16261 tcttcaaatc cacctaattg aatttctgta cgacgatcat caccgaaacg atctctaatt
    16321 tcagtcaatt catctctaac taactgtaat aacacttctt catcagctaa gattgcttct
    16381 aattcactaa tataatttaa taactcatta tattcagctt caattttgtc tctctctaaa
    16441 cctgttagac gtcttaaacg catgtctaaa atagcttgag cttgtttttc agaaagtttg
    16501 aagcgttgtt gcaagctttc cattgcaact ttatctgtat ctgactcacg aatcgttgaa
    16561 ataatttcat cgatatggtc aagtgcgata cgtaatcctt ctaaaatgtg ggcacgatct
    16621 ttagctttac gtaagttgta ttgcgtacgt cttctaacaa ctgtcttttg atgctctaaa
    16681 taatgtacca acgcttcttt taaattaata agcttcggtc taccatttac aagtgcaatc
    16741 atattcacac caaatgatgt ttgaagtggc gtttgtttgt ataagttatt taaaatgaca
    16801 ctagcatttg catccttacg cacatcaata acgacacgca caccagtacg taaacttgtt
    16861 tcatcacgta aatcagtgat accgtcaatt ttcttgtcac gaacgagttc tgcaattttt
    16921 tcaatcatac gagccttatt cacttggaaa ggaatttcag tgacaacaat acgttgacgt
    16981 ccgcctccac gttcttcaat aactgcacga gaacgcattt gaattgaacc acgacctgtt
    17041 tcatatgcac gtctaatacc actcttacct aaaataagtc cagcagtagg gaaatcagga
    17101 ccttcaatat cctccattaa ctcagcaatt gaaatatcag ggttcttact taagctaagt
    17161 acaccattga ttaattctgt taagttatgt ggtggaatat tcgttgccat acctaccgcg
    17221 atacctgatg caccattggc taacaagtta gggaatcgag caggtaagac tgacggctct
    17281 ctttcattac catcatagtt atcgataaaa tctattgtat ctttattaat atcacgtaac
    17341 agttcaagtg tgattttagt catacgcgct tcagtataac gcattgctgc tgcgccatct
    17401 ccatccattg aaccaaagtt accttggcca tcaacaagcg gataacgata actgaaatct
    17461 tgagccatac gtaccattgc ttcataaata gatgagtcac catgagggtg atatttaccc
    17521 attacgtcac caacgatacg tgctgatttt ttatatgatt tatccggtgt cataccttgt
    17581 tcatttaatc catatagtat acgacgatgt actggtttta aaccgtcacg aacatctggc
    17641 aatgcacgag caacgataac actcatcgca taatctaaaa atgattcacg catttcactg
    17701 gtaatatttc gttcatttat tcttgattga ggtaattcag ccatcaagag ttcctccttc
    17761 aaaagttcag ttcacagcgc ttagaagtct aagtttgcat aaactgcatt atcttctata
    17821 aattgtctac ggttttctac aacatcaccc attaacattt caaatgtttg gtccgcttca
    17881 atcgcatctt caagttttac ttgtaaaaga gcgcggtgct cagggttcat tgttgtttcc
    17941 cataattgat ctgcattcat ttctccaaga cctttgtatc gtgcaataga ccattttggt
    18001 gttggattca attcagattt aagtttatca agttccctat cattgtatac ataatacttt
    18061 tgtttacctt gtgtcagttt atacaacggt ggctgtgcaa tatacacata gcctgcttca
    18121 attaacggtc tcataaatcg atagaagaat gttaataaca atgttctaat atgcgctcca
    18181 tccacatcgg catcagtcat aatgacgatt ttgtgatatc ttgctttcgc tagatcaaag
    18241 tcgccaccga ttcctgtacc aaatgctgtg atcatttgac gaatttcatt gttattcaaa
    18301 attctatcta atcgtgcttt ttcaacattt aatatcttac ctcgtaatgg taaaatcgcc
    18361 tgcgttctag agtcacgacc agattttgta gaccccccgg cagagtcccc ttcgactaag
    18421 aaaatctcac attcttcagg acttttacta gagcaatcgg ctaatttacc tggaaggctt
    18481 gctacatcta acgctgattt acgacgtgtt acttcacgcg cttttttcgc agcaacacgt
    18541 gcacgtgccg ccataatacc tttttcaacc actgtacgtg cgacttgtgg attttcatat
    18601 aaaaatcgtt caaagtgctc tgagaataat ttatctacaa cttgacgcac ttcagaatta
    18661 cctaattttg tcttcgtttg accttcgaat tgaggatcac catgtttgat agatataatt
    18721 gctgtcatac cttcacgtgt atcttcacca gaaagtctat ctttttcttc tttcataatc
    18781 ttgctactta aaccataact atttaagaca cgcgttaatg cacgtttgaa tccgtcttca
    18841 tgcgtaccac cttcatacgt atgaatgtta tttgcgtaag ttaaaagatt tgtggcatat
    18901 cctgagttat attgaatcgc aatttctact tcaatatcat ctttagattg atgaatataa
    18961 attggctcat catgaatagg ttctttattt tcgttcaata actcaacgta cgatttaata
    19021 ccgccctcat agtgatagga gtcttctcta acgttttctt catcacgttc atctcttaat
    19081 gtgatttgaa ttcctttgtt taagaaagca agctctctaa tacgctgctg taatgtttca
    19141 tagttgtata cagttgtctc tgtgaagatt tctccatctg ctttaaaacg aatgacagta
    19201 cctgtcttat cagttgtgcc aacttctttt aagtcaaatt gaggtacacc ttttttatat
    19261 gcttgatgat atatagtctc atttctgtgt acatatactt ctaagtcttg tgacaatgcg
    19321 tttacaactg atgaaccaac accatgtaaa ccaccagata ctttgtatcc gccaccgcca
    19381 aatttaccac cagcatgtaa aacagttaaa ataacttcga cagctggacg tcccattttt
    19441 tcttgaatat caactgggat accacgtccg ttatccgtta ctttaatcca gttatctttt
    19501 tcaataacaa cttcaatttg atttgcataa ccagctaatg cttcatcgat actattatcg
    19561 acaatttccc acactaaatg gtgcaaacct ctctctgaag tcgatcctat atacatacct
    19621 ggtcttttac gtactgcttc taaaccttct aatacttgta tttgcccagc accataatta
    19681 tccgtgttgt ttacatctga caatgcagtc accatcgctt tctgttactt tataatttca
    19741 ccttgattaa tacgatacaa tttagcgtta ttcatgattt catgatcaat accatctaca
    19801 gatgtcgtag tgacaaatgt ttgtacttta tgctgaatcg tacttaataa atgcgtttga
    19861 cgcgaatcat ctaattcact gagtacatcg tctaataata agatgggata ttccccaact
    19921 tcgatattca ttaactcaat ttcagctaat ttaatggaca aagccgttgt acgttgctgt
    19981 ccttgagaac catatgtttg agcatccatg ccattcacat caaaacttat atcatctcga
    20041 tgtggtccga ataagctaat gcctcgttct ttttctcttt gcatattatc gctaagaata
    20101 gacataattt cttcaagtcg tgccgcttca ttttgagcat aatcaaattt aagactaggt
    20161 aaataattca gcgacaacgc ttctttatca tttgtgatac cagcatgaat cggtttagct
    20221 aacgactcta gctcttgaat aaaatgtgca cgtttatcag ttactttcat tgcatattca
    20281 gcaaactgct gatttaatac ttccaacatt gttaagtcct ttttttggcc taattgtaac
    20341 tgctttaagt aattattctt ttgctttaaa atacgttggt attgagctaa atcatttaag
    20401 taaacagcag aaatttggcc caactccata tctataaagc gtcgtcttat ttgaggagag
    20461 ccttttacaa tattcaaatc ttctggcgca aatagaacca cattgaggtg tccaatatat
    20521 tgagttagac gactttgctc taagtgattc actttgactt gtttaccttt tttagttata
    20581 aacattgtta atggcatcgt gccgtgtcta taactaagct caccttctat tttagcataa
    20641 tcagcattaa aacgtatgag ttccttatca ttactcgttc tatgactttt tgctaaagct
    20701 aaggtataaa ttgattcaag taaatttgtc tttccttgtg cattttctcc aatgaggata
    20761 ttcacgtcag gatgacattt caacgtaacc tcatcatagt tacgataatt ttctaattgg
    20821 agtgtattta acttcattgt tcaccttgat gaatgattaa gaaagaacca gcatcttcag
    20881 gtaattctgg gatatctata cgatcttgat gttctaactt tttaccgcga cgtgtttcac
    20941 gcactccatt aattaatact tcaacgtctt gcaagaacca ttttgcttga ccaccagatt
    21001 caataatccc ttctgttttt agaaattgac ctaaattaat gtctccttct actacaactt
    21061 cttgaaccaa aataatcact ccatttcttt gtatgtcatt aatatattat accttttttt
    21121 gtcacaaatt tcatttaaaa tagaggtaga aaccgtttac atataaataa ttaattctta
    21181 ttttttggca atactgtcat tttcaatttt ttaacgctat ttaacggcgt ttaactttta
    21241 tattcaactt gttattgact attaaaattt taaatttaaa gggcatataa gcacacttat
    21301 taaattttgg atatatttgt cataaattta attggcgtct tttatttgct gtttaaaatg
    21361 ctgccaaatt tattttacat acgcatctag aattattttt gccaaaaaat aaaggatgac
    21421 gttttaatta atcacgtcat cctttattta tatttatttt tagtaagttc tgattggtaa
    21481 aattaattgc gttaccgagt cgtcaccttt tggttttaga ataaatggtt tcattgtacc
    21541 gaagaattca acttcaacct catcattatc gattgctttt aaagcatcca tcatatattt
    21601 agagttgaat gaaattttca ggctaccacc ttcaacatcg tttgcatcaa cttcttcttt
    21661 tacagtacca atttctggtg atgtagaaga taattcaaca acgtcatcac ctgtacttaa
    21721 tttaataacg ttgttaccac cttcacgtgc taataaagag gcacgatcaa tcgcatgata
    21781 aaactcccca ttgtctatac ttaatttaat ttcatagttt tcagggaata aacgtgttgt
    21841 atcaggataa tgtccttcta ataatcgaga aataaagttc acatttccaa ctttaaataa
    21901 aacttggttt gaagcaaaga agatatcaat gtcttcttca ttgtcagaca taattttatt
    21961 taattcagct aaagccttac ctggaatgat gacatttttg ttttcagaaa catcttctaa
    22021 ctgcaacttt cttacagcca agcggtgtga gtcagtcgct gtgcatatta attcattttc
    22081 ttgtataagc cagttcacac cagttagtac tgggcgtgtt tctgaggtgg acactgcaaa
    22141 atttgtttgt gcaatcacgt ttttaagcac ttttaccgac aattgaattg cgtcatctct
    22201 agaaacttga ggtaataaag gatattgatc tggatctaag ccacttaaat taaattcaga
    22261 atgacctgat gtaattaatg tctggaattg ttcatttgta gataatttaa catctttacc
    22321 aggtaatttt tttataatat caacaaagaa tcgtccagga agtactactg agcctgtttc
    22381 tgaaatattg acaatatctt cgccatctac agttttagga atagtgattt ctattgaaat
    22441 ttcagagtct gaaccagtta atataacttc atgttctttc gcatcgattt tgataccagt
    22501 taatataggt aatgttgttc ttggtgaaat agcttttaat gtgtcattta attgtgtaat
    22561 aaaataatct cttttaatag tgaattccat cattaattaa aactccttcc agtcgtttat
    22621 atataattat ataggtttta agttcttagt aatagtagta ggtgctgtgg attggtggat
    22681 aagtcttaaa aacatagatt tggcgaagtt atacacatgt tgataacttg tgtatggtgt
    22741 gtatgacttt taaacattat ccacaaaatg agttttgctc atcttgccac attagcatat
    22801 agaaatgtaa aaaatgattg ttatcattag tgctgttata taacagatta catttcccaa
    22861 agtttcctac ttatacattt cttatttctt tttcaagatt ctctacttct tgtttaaaaa
    22921 taggatcttc ttttaaatct ttagatattt tttcatgagc atgaatgacg gtcgtatgat
    22981 cacgcccacc aaattcttca ccaattttag gtaatgagaa atctgtaagc tctctagaca
    23041 agtacatagc tatttgacgc ggatatgcaa ttgactttgt acgttttttt gcactgaaat
    23101 cttcaattct aacattatag tactggccta caattttttg aatatcttgg atggtaatct
    23161 ttttagattt tggtgcttga atgatatctt ttaaagcttc agcagttaat tcagttgtaa
    23221 ttggttttcc taataattgt gaatatgcaa gtaaacgtgt taatgcacct tctaattcac
    23281 gaatattaga ttgaatttga tttgctatat aatttaaagc ttctggtgga atatctaatt
    23341 tttcttcttc aattttcttc tgcaaaattg ccattcgagt ttcataatct ggtggcgtaa
    23401 tatcaacaat tagcccccat tcaaagcgtg aacgtaatcg gtcttctaat tgtgcaattt
    23461 cctttggtgg tcgatcactc gaaataacta tttgcttgtt attctgatgc aattcattaa
    23521 aagtatagaa aaattcttct tgtgtttgta ccttgttttg tatgaactga atatcatcga
    23581 ttaataagac gtcgatatta cgatatcttt ctctgaaagc ttcaccttcg ttatcacgaa
    23641 ttgatttaat aaattcattt gtgaattttt cacttgatgt gtaaatcact ttggcatctg
    23701 gattattatc taaaacatga tgaccaatgg catgcattaa atgggttttt cctaaaccaa
    23761 cccctccata gataaataat ggattgtacg ctttggctgg tgcttcggct acagctaaac
    23821 ttgctgcatg tggaaagcgg ttaccaggtc cgattacaaa agtgtcaaat gtgttatggg
    23881 cattgaattg ctctctacca agcacatgat tatcctcagt tgtttcagta gaaggttttg
    23941 ttgtttcttt tggagtagca gtttcattat tactataatt tgctaattct tcagtagtaa
    24001 taaagtgagg tttaacttca tagcctacaa catcaaataa gattgcttgg ataatttcag
    24061 catattgttg atttaaccaa tttgcattaa aaggaatact cgataatacg atagcttcac
    24121 catctttaat cgtgtaaagc tcagtatctt ttaggaaagt tgagtaactt acagctgata
    24181 atttttcttg agcaatttca agcacttttt cccaaatttc tttttccgac ataaatttac
    24241 tccatctttc ttaaattttt aagttataca caaaaataac agccgtggat aacttctaaa
    24301 cacaccatgt taataattat ccacaagtta ataacaatcg gtggataact ttatcctgct
    24361 tattagttat gcacagtatt tttatgtaaa tatcaataaa taaatcctta taaaatcatg
    24421 agttggacga cttatccaca caattatact ttttcagttc tctatatgtc aaatacctgt
    24481 gaatatgttg ctaaaaatag tataactttg tgtgtaattt ctaattatcc acaattctga
    24541 aaactataaa tgtgcataag tggataactt ttccttctat agaatatctg ttagtgagtg
    24601 tatcaaaaca gcttgggaaa taatttataa agtatgtata agaactgtat aaggtattca
    24661 aacattgtaa acactcatgc ttcggaccaa actcatggtg atgttatgaa atttgattgc
    24721 tcgcatcgtg tatttctatc tttaatcgat aaaagtaaaa aaattgagca gtagtataaa
    24781 atgaacttgc gttataaaaa ggattttgtt atattgtagt agttgcttga attatgacta
    24841 gataatcaat gagctaatac gagaatttta atctaagaaa cgcaaaatct tatctattaa
    24901 agtgatcaga gtctgcataa tctggtatat tagataactg tataaagatg gaggtgtttt
    24961 gcatggtaaa acgtacttat caaccaaata aacgtaaaca tagtaaagtt catggtttca
    25021 gaaaacgcat gagcacaaaa aatggccgta aagttttagc gcgccgtcgt cgtaaaggcc
    25081 gtaaagtttt atctgcataa gatcactgac tcatcagtga tctttttttt cgtttaaatt
    25141 aagaataaat agaaatttat gttataagct caatagaagt ttaaatatag cttcaaataa
    25201 aaacgataaa taagcgagtg atgttattgg aaaaagctta ccgaattaaa aagaatgcag
    25261 attttcagag aatatataaa aaaggtcatt ctgtagccaa cagacaattt gttgtataca
    25321 cttgtaataa taaagaaata gaccattttc gcttaggtat tagtgtttct aaaaaactag
    25381 gtaatgcagt gttaagaaac aagattaaaa gagcaatacg tgaaaatttc aaagtacata
    25441 agtcgcatat attggccaaa gatattattg taatagcaag acagccagct aaagatatga
    25501 cgactttaca aatacagaat agtcttgagc acgtacttaa aattgccaaa gtttttaata
    25561 aaaagattaa gtaaggatag ggtaggggaa ggaaaacatt aaccactcaa cgcatcccga
    25621 agtcttacct cagacaaacg taagactgac cttagggtta taataactta ctttaaaatg
    25681 tattcacatc gttgaaggag gcgagtagca atatggattt agatacaatt acgagtattt
    25741 caacaccaat gggtgaaggg gcaattggaa ttgttcgatt gtctggaccg caagccgttg
    25801 aaattgctga caaattatat aaaggaaaac atcttttaaa tgatgttcca tcacatacga
    25861 ttaattacgg tcatattatt gatccagagt ctaaagaagt ggttgaagaa gttatggtgt
    25921 ctgtgttaag agcgccaaaa acatttacac gcgaagatat tatagaaatt aattgtcatg
    25981 gtggtatttt aacgattaat agagtgctgg aacttactat gacatatggc gctagaatgg
    26041 ctgagccagg tgaatttaca aaacgtgcct ttttgaatgg tcgtattgat ttatctcaag
    26101 ctgaagcagt tatggacttt attcgctcga aaacagatag agcttctaaa gttgcgatga
    26161 atcaaattga aggtcgtcta agtgacttaa tcaaaaaaca acgtcaatct atattagaga
    26221 tactcgctca agtggaagtg aatattgatt atcctgaata cgatgatgtt gaagatgcga
    26281 ctactgaatt tcttttagag cagtctaaag aaatcaaaca ggaaattaat cgtttattag
    26341 ataccggtgc gcagggtaaa attatgcgtg aaggtttatc tacagttatt gttggtaaac
    26401 caaacgtagg taaatcatcg atgttaaata atttaataca agataataaa gcgattgtaa
    26461 ctgaggtagc aggtactaca agagatgtct tagaagagta cgtcaatgtt cgtggcgtgc
    26521 cattaagatt agttgatact gctggtatac gcgagacaga agatatagta gagaagattg
    26581 gtgttgaacg ctctagaaag gctcttagcc aagcagactt aattttattt gtattaaaca
    26641 ataacgaagc attgactcaa gaagattaca cattatatga agtggttaaa aatgaagatg
    26701 taatcgtaat tgttaataaa atggatttag agcaaaacat agatattaat gaagttaaag
    26761 atatgatagg tgatacgcca ttaattcaaa cttcaatgtt aaaacaagaa ggtattgatg
    26821 aattagaaat acaaattcga gatttgttct ttggtggaga agtacaaaat caagatatga
    26881 cttatgtttc taattcaaga catatttcat tattaaaaca agcaagacaa acgatacaag
    26941 atgcgattga tgcagcagaa tctggtgtgc ctatggatat ggtacaaatt gatttaacta
    27001 gaacttggga aatattagga gaaattattg gtgagactgc aagtgatgaa ctcatcgatc
    27061 agttattcag tcaattctgc ttaggtaaat agtaattgaa atagacggaa taccgtctta
    27121 agaaggctag taagatatca aataaggagg tttatattgt ggttcaagaa tatgatgtaa
    27181 tcgttatagg tgcgggacat gcaggtgtag aagcaggttt agcatctgca agacgtggtg
    27241 ctaaaacatt aatgctaaca ataaatttag ataatattgc atttatgcca tgtaacccat
    27301 ctgtaggtgg accagctaaa ggtatcgttg ttcgtgaaat tgatgcttta ggtggacaaa
    27361 tggcaaaaac aatcgataaa acacacattc aaatgagaat gttaaataca ggtaaaggac
    27421 ctgctgtaag agcactaaga gcgcaagcag ataaagtact ttatcaacaa gaaatgaaac
    27481 gcgtgattga agatgaagaa aatttgcata taatgcaagg tatggtagac gaacttatta
    27541 tagaagataa tgaagttaaa ggtgtacgta caaatattgg tacagagtat ttatctaaag
    27601 cagtaattat tacaacggga acatttttac gtggtgaaat cattttaggt aatatgaagt
    27661 attcaagtgg accaaatcac caattaccat caatcacatt atcagacaat ttaagagaac
    27721 ttggttttga tattgttcgt tttaaaacag gtacaccacc gcgtgtaaat tcaaaaacaa
    27781 ttgactattc gaagactgaa atacaaccag gtgacgatgt aggtcgtgca ttcagctttg
    27841 aaacaacaga atatatatta gatcaattgc catgttggct aacgtatact aatgctgaaa
    27901 cacacaaagt tatcgatgat aatttacatc tatctgcaat gtattcaggg atgattaaag
    27961 gaaccgggcc acgttattgc ccttcaattg aagataaatt tgttcgattt aatgataagc
    28021 cgcgacatca acttttctta gagcctgaag gtcgtaatac aaatgaagta tatgtgcaag
    28081 gattgtctac aagtcttcct gaacatgtgc aacgtcaaat gttagagacg ataccaggtc
    28141 ttgaaaaagc agatatgatg cgtgccggct acgcaattga atatgatgcg attgtgccaa
    28201 cgcagttatg gcctacactt gaaacgaaaa tgattaaaaa cttatatact gcaggtcaaa
    28261 ttaatggtac atctggttat gaagaagcag caggacaagg attgatggca ggtattaacg
    28321 ctgcaggtaa agtgttaaac acaggcgaaa agatattaag tcgttcagat gcatatattg
    28381 gtgtcttaat cgatgatctt gtaactaaag gtactaatga accttatcgt ttactaacat
    28441 cacgtgcaga atatcgtttg ttactacgtc atgataatgc tgatttgaga ttgacggata
    28501 tgggatatga acttggtatg atttctgaag aacgatatgc acgttttaat gaaaaacgtc
    28561 agcaaattga tgcggaaatt aagcgtttat cagatattcg tattaaacca aacgaacata
    28621 cgcaagcgat tattgaacaa catggtggtt ctcgcttaaa agatggtatt ttagctatcg
    28681 atttattacg cagacctgaa atgacttacg atataatttt agaaatttta gaagaagaac
    28741 atcaattgaa tgcagatgtt gaagaacaag tagaaataca aacaaaatat gaaggttata
    28801 tcaataaatc actacaacaa gttgagaaag ttaagcgtat ggaagagaag aaaattccag
    28861 aagacttaga ttatagtaag attgatagtt tggcgactga agcgcgagaa aaattatcag
    28921 aagtaaaacc tttaaatatt gcacaagctt ctagaatatc aggggtaaat ccagcagaca
    28981 tatctatatt attgatttac ttagaacaag gtaaactcca aagggtgagt gactaatgac
    29041 tgtagaatgg ttagcagaac aattaaaaga acataatatt caattaactg agactcaaaa
    29101 acaacagttt caaacatatt atcgtttact tgttgaatgg aatgaaaaga tgaatttgac
    29161 aagtattaca gatgaacacg atgtatattt gaaacatttt tatgattcca ttgcacctag
    29221 tttttatttt gattttaatc agcctataag tatatgtgat gtaggcgctg gagctggttt
    29281 tccaagtatt ccgttaaaaa taatgtttcc gcagttaaaa gtgacgattg ttgattcatt
    29341 aaataagcgt attcaatttt taaaccattt agcgtcagaa ttacaattac aggatgtcag
    29401 ctttatacac gatagagcag aaacatttgg taagggtgtc tacagggagt cttatgatgt
    29461 tgttactgca agagcagtag ctagattatc cgtgttaagt gaattgtgtt taccgctagt
    29521 taaaaaaggt ggacagtttg ttgcattaaa atcttcaaaa ggtgaagaag aattagaaga
    29581 agcaaaattt gcaattagtg tgttaggtgg taatgttaca gaaacacata ctttcgaatt
    29641 gccagaagat gctggagagc gccagatgtt cattattgat aaaaaaagac agacgccgaa
    29701 aaagtatcca agaaaaccag ggacgcctaa taagactcct ttacttgaaa aataatgcat
    29761 aatcctttac aactaacata aaaggagcga atggataatg aaaaaacctt tttcaaaatt
    29821 atttggtttg aaaaacaaag atgacatcat tggacatatt gaagaagatc gcaatagtaa
    29881 tgttgaatcc attcaaattg aacgtatcgt tcccaaccgt tatcaaccaa gacaggtgtt
    29941 tgaaccaaat aaaattaaag aacttgctga atcaatacat gaacatggtt tattacagcc
    30001 tattgttgta agaccgattg aagaagatat gtttgaaatt attgctggag agcgccgatt
    30061 tagagcaata caatcactaa atttacctca agcagacgtt attattcgtg atatggatga
    30121 tgaagagacg gctgttgttg cattaattga gaatattcaa agagaaaatt tgtctgttgt
    30181 tgaagaagcg gaagcctata agaaattatt ggaaattggt gatacaacgc aaagtgaatt
    30241 ggcaaaaagt ttaggtaaaa gtcaaagctt tattgcaaat aagttgcgtt tattgaagtt
    30301 ggcgccgaaa gtactacttc gcttaagaga aggtaaaatt actgaacgcc atgcgagagc
    30361 ggtattatca ttgtctgata gtgaacaaga agcgttgatt gagcaagtca ttgcacaaaa
    30421 actaaatgtg aaacagactg aagatagagt acgccaaaaa actgggcccg aaaaagtcaa
    30481 agcacaaaac cttcgctttg cacaagatgt cactcaagca cgagatgagg taggcaaaag
    30541 tatccaagcg attcaacaaa caggactaca tgttgagcat aaagacaaag atcatgaaga
    30601 ttattatgaa ataaaaattc gaatatataa acgttagtag taggatgtcg tatacatgat
    30661 gactaacaca taaaagacaa agctaagatc ataacagctt tgtctttttt tgttttacgt
    30721 gaaacataaa aatttatatt tatatgttga tttgaaaatt acagttaatg gtaattttca
    30781 ataatttgag ctggatatac cttcatttga accctttatt gctagtttat gaaagtatca
    30841 tgaaagcttt atgaacatcg cttgagttgc ctttacagta gaaaatttaa gttttacact
    30901 ttgtgtgaat gatacgtttt gtattgaatt aattatagaa aggtacgttg aagatgtttt
    30961 caattggaag tgcaatactt cattttgtca ttggtggtat cgctgttgca ttagcttcaa
    31021 ttattgctga taaggtaggt ggtaagttag gaggtattat agctactatg ccggcagtct
    31081 ttcttgcggc tattatcgca ttagctttgg atcatcgtgg tacacaatta gtggagatgt
    31141 cgatgaatct tagtactgga gcaattgtcg gtattctgtc ttgtatatta actgtatttt
    31201 tgacatctct ctatattaag cataaaggtt atcggaaagg cgcaatattc acagttgttt
    31261 gttggtttgt tatttccctt gcaatattca gtattagaca tttatagttt ggaaaatgcg
    31321 tgataattag ttgtattcag ttattaagta ataaattttt ggaggcagaa catcatgaaa
    31381 ttaacattaa tgaaattttt tgtgggggga tttgcagtat tattaagtta tattgtatct
    31441 gtaacactac cttggaaaga atttggcggt atatttgcaa cgtttccggc agtattttta
    31501 gtgtctatgt ttattacagg tatgcaatat ggtgataaag tcgctgtgca tgtaagtcgt
    31561 ggcgcagtgt ttggtatgac aggggtatta gtttgtattt tagttacatg gatgatgtta
    31621 catatgacgc acatgtggtt gattagcatt gttgttggtt tcctaagctg gttcatcagt
    31681 gcagtatgta tttttgaagc agtagaattt atagcacaaa aaagattaga aaagcatagt
    31741 tggaaagctg gaaaatcgaa tagtaaatag tgtgaacgta atctcttaaa taggactaac
    31801 ttcgcaagca ttgaatagca tggaaaagtt gcatcattaa taaatgaaat tcaagttggc
    31861 attgagaaaa ttacaagcgc gtaatcatac aggtctgtct tgaggaagtc ttcaaaccct
    31921 gatgttgtcg tatgtcaaaa catttagtca atcataaagg tgactttaac taagtttatc
    31981 tgatagtctg attgtaatga ttgtactaat tgactggagg cgtatgtaat tgaatctgag
    32041 taaacaaatt aaaaaatata gggaacgaga tggttattca caagaatatc ttgctgaaaa
    32101 gttatatgta tctaggcaga gtatttctaa ttgggaaaat gacaaaagct taccagacat
    32161 acataactta ttaatgatgt gcgaattgtt caatgtaact ttagatgatt tagtaaaagg
    32221 gaccattcca tttgtacctg atattaaagc gcaacgaagt cttaacttat ggacatatgt
    32281 gatgcttatt ttcatgacat tagctgcaat tttaatggga cctttagttg tttattggaa
    32341 ttggacttgg ggtgtaacgg tggcaatcat tttgggaata ggtttttatg catctatgaa
    32401 aatagaagat ttaaaaaaag tgcataaaat ggacaactac gatcgaattg ttgcttttat
    32461 gaatggaaaa gatcctagtg aagtacaaac gactaaagct agaaatacga tgacaaatgc
    32521 gctttctatt atatcagtaa ttggtatact cagcctcata attttcctta gtgtgtattt
    32581 ggcaaataag tttttataaa tcatcatcgt attgtctcat attatccaaa atagcataaa
    32641 aaaataccaa caagatttag aaccttgttg gtaatcaaag cgattcattt ataatgagtc
    32701 gttttatttt gtaagattaa acagtttgta cgttaactgc ttggtctcca cgttgacctt
    32761 cagtgatttc gaaagtaact ttttgacctt cttctaaagt tttgtagcca tcgctagcga
    32821 tacctgagaa atgtacgaat acgtctccgc cattttcttg ttcgatgaaa ccaaaacctt
    32881 tttctgcgtt aaaccatttt actgtaccgt tattcatata gaaaacctcc atgtgctttt
    32941 gcacttaata tttgtaacaa attcataact aaaaaagagg atattctaaa caaatacact
    33001 acaatttaat tcacgagctt ttattacgta agaccaacta tacgctcata ttggcataat
    33061 gtacagtgtt ttttgaaaat aaattaaaaa agatttttaa aaaccttaga aacgttgatt
    33121 taaaggggtt tataaaaata aaaaaattgt agtcttttat ggtgtttgct agttttcaaa
    33181 gtgacatatc gtttaaacat gatgatttta taagcaatcc ataaaaaaca agcagcgata
    33241 aacgctactt gttgatatta aaatctgact tgaaaggtca tagcaatgtt ctataccgat
    33301 ggaatgtgct tacttgcctt tttcttcacg acgttttaaa taataggcgc cacctaataa
    33361 accagctgga atacctataa ttggtgttgt aactacgctt aagacaataa caatcattgc
    33421 taaagcaatg acgttatacc aagttacagt caaatttttc aaatcctcgt atgattgttt
    33481 tactaattct ctaaatttca tgattcaatc tctccttttt tataaatcag ttaatagtta
    33541 tactgcattg cttaaaatca aaatctcaat gaagcatctt ttaatcataa gtgttaaatg
    33601 gttttcttaa tcaatttgtt tgaatgtaca aaagcaatta aagcaaaagt aatgtaaatg
    33661 agtgtgtata caacaattac tacaataacc ggcatgaagg aaatattacc catcaatttc
    33721 ataaatgcta ttgcggcgaa tactgcatgt aatatagcaa tcaataatgg taaaccgaag
    33781 ttgaaagtaa tttttaataa taatccctta agcatatctg tatgcgtaaa gcctatgcgt
    33841 tttaatattc tgaagttact tagttcatcc tcagtttcat ccatttgttt aatataaata
    33901 atacatccag ctgctactaa aaatgctaat cctaaaaatg atgtaacaaa tattagaata
    33961 ccgttagtag catcgacctc ttttttcatt tcatcatacg tgatgacttt gtctccaaac
    34021 tgttttgcaa ttgtttgagc tttttccttt tgtgatgttt gtttaatatc atatccataa
    34081 aaagtatgaa cgttattttg tgttttcaac tgctgatact tttcaggact tacttcgatg
    34141 acaggtgagt tgaagcttag atttaaagga taaaccttac ctttgtcttc ttgtgtaaca
    34201 cggaaagttt cattcttagt accttttact actaaatctt tgtttaaatg gatattaata
    34261 atgttaggca gcgattttgt atttgtaatg atggcattgt tgcctgttaa cttgttattt
    34321 gcacttaaaa tagaattcgt gcgacctgaa tcgctaccat tttccaaagt aataacctga
    34381 tctttaacat tatctacagt aatagtttcg taagcatttt tagaaaatgt aatttgttgt
    34441 tggcttagtt tagtttcaaa ttgtttagca tcttgagtag cgaccacgtt aaattcattt
    34501 ggtgccatag atgtaagggt ttgatctgta tttgatttag ataatgccgc gaaacacaat
    34561 acagttactg taactgcaga aataattgca atgatagtta aagacatcgc atttttcttc
    34621 attcggtaca taatagacga tgtaaataca acatctgtaa tagatacgcg tccattttta
    34681 gattttttca aagttttaaa aattagtgac acggaacttc tgaagaataa ataggcgcct
    34741 actaccgtta aaaataaaat gataaacggt gatgtcatag ccatagttag tgctttaaat
    34801 gttccaaaca tttctgtcgc catataatag cctagtgcaa tcatagcaat acctaatacg
    34861 cctgaaatga cttctataac tgtaactttc gcagttgtaa catcagtttt aattgagtct
    34921 ttcatcattg ataagatact acgtcttttt agaaataaag cactttgaaa taaaatcagt
    34981 acatacgcaa taattagcat gaaaatagtt aaaacaaggg ccataggttc gaaatgtatc
    35041 gataagttaa tcgataacga catcaattta gatactatgg aaagcaataa ttgtgcacct
    35101 gcaatgccac ataatacacc gacaacacct gtgattaaaa atacaatcat ttgttcaagt
    35161 gctaacattt tcaaaatgtt ttgtcgtgtt aaaccaatca actgaaatag cgcaaattca
    35221 cgtgtacgac gtttcacgaa taaatgattg gcatacatta aaaagatgac aataatgata
    35281 aataaaaata ttgatccgac taaagcacct ttcttaatga tggccatcga gtcgtcatta
    35341 tttacacctt tagtaaactg taaggttgta aaactgaaat ataagacgat gctaaaaaat
    35401 aatgaaaata aatacattgc ataatgtttt aagttttggc gtaaattttt gaaaacgata
    35461 tggttaaatg tcatttgaga caccacctaa tactgattga agatgtacaa tgtcttcata
    35521 aaaggcctgt ttagaacgtc cttcctgata aagttgtgta tgaatttggc catcttttag
    35581 catgatgaca cgttctgcaa aacttgctgc aaccggatca tgtgtaacca tgacaatagt
    35641 tgtatcaaac gatttattca tttcttccaa acgttgtaat aagtcatttg cacttttcga
    35701 gtcgagtgcg cctgttggct catctgcaaa tatgatttgt ggtttgtgaa caaatgctct
    35761 cgctgctgca gttctttgtt gttgaccacc agataattcg ctagggtatt tatttcctag
    35821 gtcataaata cctaatgctg tcgtgatcgc tttgtaattt tcttccattg ttgccttcga
    35881 cattttttga acagataaag gtaacataat gttttcttta acagttaatg tcggcagaat
    35941 actgtaatct tggaagatga aacctaatga ttctttacgg aatttggcaa gtgctttttg
    36001 attaagttta ttaagctctt gtccgttagc aatcacgcta ccgctagaaa tttggtcaat
    36061 tgaacttagt acatttaata aggttgtctt acctgatcca gaaggcccca taatcgcaac
    36121 gaattcgcct ttttgtatgt caaagttaat atctttaagt gcttgaaatg tgtgcttttt
    36181 accgtatgtt tttgaaacat gttgcactga taatattgtc atagtctcac tccttttgta
    36241 tttaatttca ttttaaataa tgtttggagt agtagccttt atctaaactt acaattcaat
    36301 gtttgaacct tacagagttg aaagctatcg ctacgtagta gatttttgag tgaggataca
    36361 gattcatcgt acatattaga caaaagcaat ggtgctttct aagtgatgat gtttgtgtaa
    36421 attgagaaaa gggaatttaa ttattgtata ataaattttt tgtaaaaatt aaaagagggc
    36481 tttatttgaa aggaattgat tgttatggca aaaggaaacc aaggtattaa atggtctagt
    36541 ttaataatgg gtgtattatt attaatgttg gcagtcgtta tttttacatt tccaattgaa
    36601 aatttttatg ctattacctg gttgattgga ctgtttgtat taattaacgg tgtgattcaa
    36661 atcatttacc gtagaaaagc aaaagcttta gtaggtggta accaaaattg gattctgttt
    36721 atggggattg tagatattct ctttggtctt attgtcattt ttaatgttgg cgctagctcg
    36781 gcatttttta tctacatgtt tgcattctgg tttattttta gttctattgc aggattattt
    36841 acgtttacgg gtagtggtag cttaaaacta atttcagtga tttttaattt attaggtatt
    36901 gttttcggtg tcattttatt atttaatcca ttaatgggta tcgtctttat ttcgacgatg
    36961 attgctattg catttgtatt cgtaggtgtc atttatgttg tagatgcact tgcttaagta
    37021 aaatgaagcg gttcaaaaga agggtgtgac atgaagtttg tgtcatatcc tttttgttgt
    37081 gtttatgaag cataaaaacg gggcgctacc tacaataagt aagatacgcc catattttta
    37141 tattttacta ttattgtttt tcaatacgat taatagttat atctagtcca aaatattttt
    37201 ctaaaaaatg tttatagtta tttttagtga cagctaactt ttctgagcta ccattccttg
    37261 ttaaagttaa atgattttca gacattgtag cacggccaaa tgattgtggc attgtaatta
    37321 ataaatgctg tacaaatatt gaatctgggt tggtttgatt atattcgatg ttgctattaa
    37381 aatcttctat ttgtttaggt ttgaattcag cttcatattt tgtatgccaa tgatcatttt
    37441 cgaatttttg aacatagaaa atatccttgt cttcgttgtt aaaaatagcg cggaatgtac
    37501 cactgatatc agtaattggt tgtgtatgct cagatgaagt aataggaatg gcatgtatag
    37561 gtaagtctcc aaagccaaca tcagttacat agaatacatc atttatagaa acaacaagtg
    37621 aagcatgtga accgttcaga ctacgaccgc caccgggtgt gtgaatagta gctgacatta
    37681 atgcaggatt aaatcctttt tgttgtaaat aggctttgaa aaatgtattt aactcataac
    37741 aaaaaccacc acgtttatca tgaacaattt tattaaaaag tgcatcgata tttatagata
    37801 taggcttact attttgaaca tcaatatttt caaaaggtac agttaacata aaacgtgttg
    37861 cataataatt taatgcttca atactcggtc gattataacg agatgaatca atttgtaaat
    37921 aattctctaa cttcgcaata ttcataagca tagcgcctcc tgtattaaag attataatta
    37981 aattttaaac agaaatactg aaattttaaa ttcgaaagca ttgaattttg gataaataca
    38041 ttttaaatag aaaaatacgc tctcaaaatg aagtcatctc taaaagaaac gatttaaaga
    38101 tgactactga gagcgtagca taatggaaga agtgtgcagg gtgtctaaaa atgcaacaat
    38161 acaaaggtag ttgcaagaca agttacttta tttagaccat ttgtgttcta tgcgaccaaa
    38221 cttccaaatt aaacttgaaa taagccaagt aattaaaaat aatgcaacta aaatatagcc
    38281 taaataatca aattcgatcg aaccaatgaa tgcccaaaac gcaccatgta aatctagctt
    38341 atcagcaaga atttgtagca attcaatcat cccaatcact aatgctgcca tgactgatat
    38401 cgcagtaatc gttatattgt aatagatttt gcgaatagga ttgaagaatg cccaattata
    38461 ggcatacttc attacaacac catctaatgt atccaataaa ctcatacctg atgcgaataa
    38521 aattggtaaa gataagattc cgataaacga aatggcttgt tgtgatgcgc ctgaagaaag
    38581 agcgagtaac gcaatttcac tagctgtatc aaaaccaagt ccaaataaaa agccaagtgg
    38641 caatacgtgc caactacgcg tgattaattt gaaataaggt cctacaaatc gagaaaccaa
    38701 tcctctagat tcaagtaatg catcgacttc agcttcttca atgtgttcac gacgtaattt
    38761 agcgaacaag ttaattaaag agattaaaat aattagattc aacacaccga taagcactaa
    38821 aaagaaacct gaaactagtg taccaatcgt tccaccaata tcttggaaat gcggtaattc
    38881 atctttagcc cattttacag atacccctaa aaaaacagcc attaaaaata cgacagatga
    38941 atgtccaatt gaaaaataga aacccacacc agatggatct ttgcgttgct gtaataattt
    39001 gcgaaccgta ttatctattg cagcaatgtg atctgcatca aatgcatgac gcaaacctaa
    39061 tgtatatgca agaatcccca taccaaataa gatatgatgg tcttttccag caatccataa
    39121 aaaactaaac ccaataacgt gtaacaaaat gacaatagct atgtatggta accagcttaa
    39181 gcgctcattt ttaaaaacag tcaaaatata tccttctttc ataacatgat atttaatcat
    39241 actgtatgtt caatgggcac tctagtaata agtgttcata taacaaaaat gttatgccaa
    39301 attatttgtt atataacaat atacatgtaa ccacaaaaga tttttttgcg atatatataa
    39361 tttgataaat taaccaacaa caatgtaaga tgtcactttg cttaacttgg catccttttt
    39421 atgattttca aattcaaaaa aatgagcaaa atgaatctct ttaccagttt ttaatatttc
    39481 aataccatgc atggaaccta agcacccatg tgtgatgctg gaatggatat tgagactagc
    39541 aacctgattg taatgattag atagttcttg aattaatatt tgaggtccgt atatgtcaaa
    39601 gcggccaggg acagaccaaa taaattctgt tgtaaccagt gaacgtaata attcaatatc
    39661 taatgctgct gtagcaacta taaaatctat catttgttga cgtttaggcg catgattgca
    39721 tgacacatct cctgttaact taaaaggtaa tgatgactga acttccgttt taaaatgtag
    39781 ttggtgctga aataaagctt gttccaatat tgatatagct ttaggaccaa cgccatgcat
    39841 ctccattaga gatgacttcg tatattgtga tactgcttct aatgtgtata taccttgtga
    39901 atttagcgcg cgtgttgcag gctttccaat tttaggtaaa gtcatttgta tccatccttt
    39961 atgttgtatg gtagtctttc tggcaatata ccctgatttg tacagaatta tttataaaat
    40021 taatattgtt tagtggttgg tgaataaatg tgggaaatat aaacaaaatg atatctttat
    40081 cgaaatatta ttagtaaaat cgttatatta gcataaccaa gtgagcgtaa catgttaaaa
    40141 atttgggaga gtaaggaggt atgtaagtga atacagaata taaaaaaggt atatttttag
    40201 cactcagtgc atacattctg tggggaatac tacctatata ttggcagttc gttgatgaaa
    40261 taggcgcatt tgaaatttta gcctttcgta ttatattttc agcaatattc atgattttca
    40321 tactcgcggt tggacaaaaa caacgcaatg catttcaacg agatatgaat caattgttag
    40381 gcaagcccat tcagctatta gcgattgtcg tagcaggcta tgtcattaca ttaaattggg
    40441 gtacatttat ttgggctgta acgaacggtc acgtcctaca aacaagttta ggttattata
    40501 taaatccact tgttagcatt ttgctcgcac ttatcttttt aaaagaaaga ttcaataaat
    40561 ttgaatggct agctatttta ttcgcattca tcggtgtatt atatatgacg ctcaagattg
    40621 gagaattccc aatcgtttct attatattag cgttatcctt tggtacatac ggattattga
    40681 aaaaagtagt acatattgat gccatcagca gtattacgat tgaatgtatt gttaccgcac
    40741 ctgctggact aatatacgtt atttatttat ggcagcaaca tcagatgtca tttggattga
    40801 acatgtcatc attttggttg ttattttctg gtgctattac ggcaatacca ctaatcctat
    40861 tctcagccgg ggcaaaacgt attccacttt cgttaatagg atttattcaa tacgttggac
    40921 caacaataat gtttgtactc ggcatatttg ttttcaaaga gccttttaat atagatcaat
    40981 taattacgtt tatatttatt tggacaggta ttgtgttata tagtctttct caatacatta
    41041 aattgaagaa acatccggtc gcaaaaaccc tataaaatac gaggaaagat aattatacgt
    41101 gttttcaata tcctttgtgt catgataagg tgagactgag gtatgtgtgc agtaacagcg
    41161 acagctgtat aaatttgaaa cattaaatat gaaataaatc actttcgttg aattcgcact
    41221 taaaatgaat ttaaacgaag gtgtttttaa tttcatgatt tatgagggca tacgtaatgt
    41281 taatcaacga agcgcgttta tacagaaatg tattttctgt gatgttacgc aatgatttaa
    41341 agcgttatat agatttttag tgaagcacgc aatgatttaa aggataaacg tattttaaaa
    41401 agggagtctt gatttagcaa aggttgattt taaatacaaa ttaaaaagcg cccaaatcaa
    41461 tgagattcga gcgctaattt aaatatttta aaatatacca attactttca tccaaagtga
    41521 tcctaaacca atccagataa taaagtagac gaaacctaag atcaagttca ttgtccacca
    41581 acgtttttga gtcacgtaac ctgaagagaa tagaatcggc gctgggccac tactatagtg
    41641 tgttgttgaa gctaataggt taccgaagaa acctaacatt aatgcactga ataatggtgg
    41701 tgcaccggct gcgatggcaa cgcctagtaa tgctgcatac atcgcactga tatgtgctgt
    41761 agaacttgca aataagtaat gtgaatagaa gtagaacaat attaaaatga ccagtactat
    41821 aggccagctt aagccaccaa gacttgtagc aatggattta cttaaccaag gaataaatcc
    41881 aagcttgttt aattggtcgg ccattaacac taatactgag aaccatacta atgtgttcca
    41941 agcacctgtt tcgtttaaaa tgtcttgcca tgttaagacg cctgtcaata ataacaatgc
    42001 taacgcaata aaggccgtta aagttgcatc aatatgaatg aaacttccga caatccatag
    42061 tgttaacgca acgacaaaaa taccaatcat aaatttttca gctaaagcga ttttacccat
    42121 agtcgctaat tcattttcag cccaactctt agcatttggt gtttctttaa cagttggtgg
    42181 ataaatttta taaataataa aaggtacaac aattaaggaa ataagtccag gaactaaagc
    42241 agctaggaac caattcatcc atgtaatgtg aacattagat gtgctagatg ctaaattttg
    42301 tgcaaggggg ttaccggcca ttgcagttaa aaacatagcc gcagtaatta aattaccttg
    42361 gaattctgtg aaaacaagaa atgcacccat tttgcgtgct gatccgtctt tcggtttcga
    42421 accaaatgat tcagaaagtg atttgataat tgggaacatg attccaccgg cacgcgcggt
    42481 attacttggt gtagcaggcg ctagaattaa atctacaccg acgatagaat atgctaagcc
    42541 taatgttttt ttaccaaata atttgacgaa atgaagtgcg atacgtctac caagacctgt
    42601 tttcacaaat cctctcgaaa tgaaaaatgc catagcaatt aaccaaatgc tattattacc
    42661 aaaaccagcg acagccgttt tcatgtcaac aatgccaacg agtaccatga ttgtaaatcc
    42721 aattatagag acggccccaa ttggcatcgg ttgtgtaata caagcaatga ttgtcgcgac
    42781 gaatattgcg aacatatacc atgctgttgg atccacagca tccggtttaa aaggtgtaag
    42841 tgcccaaata aggagaccta caacgatagg gagtataaac ttacgatatt taaccgtgtt
    42901 ttccatgtta aaacgtcctt ctttctatgt tttatacata tttcaattta agaataaagc
    42961 taactacaaa agatgtacag taataattaa atataaaatt aaattaacga aatcattaat
    43021 ataattattt ttcgagaatc ggtgaagaac tggtatagat ggtatttatt aaatttaaaa
    43081 gattttgaag atgaactaat atactaagaa attaattgat acaagttaac ttcatgcact
    43141 tgta
//
LOCUS       DAMZKC010000003        38929 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.003, whole genome shotgun sequence.
ACCESSION   DAMZKC010000003 DAMZKC010000000
VERSION     DAMZKC010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 38929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38929
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.003"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(104..1438)
                     /locus_tag="POB55_000095"
     CDS             complement(104..1438)
                     /locus_tag="POB55_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDI6499093.1"
                     /translation="MKNYFQFDKYGTNFKREILGGITTFLSMAYILAVNPQVLSLAGV
                     KGVSEDMKMDQGAIFVATALAAFVGSLFMGLIAKYPIALAPGMGLNAFFAFTVVLTMG
                     IPWQVGLTGVLFSGIFFAILTVTGFREVIINAIPYQMKMAVSAGIGLFITFVGLQSSG
                     IIVKNESTLVTLGHLTDPPVLLAIFGIVITVILYAIKLPGSIFIGMIITAIVGMFTGL
                     IQMPSGIVGKIPSIEPTFGAAFEAFKDPSQLLTIQFLIVILTFLFIDFFDTAGTLVAV
                     ATQAGIMKDNKLPRAGRALFSDSLATIVGSVFGTTTTTSYIESTSGVAVGARTGFASI
                     VTGCCFLLALFFSPLIAVVTSAVTTPALVVVGVLMAANFAEINWKSFEVAVPAFITII
                     MMPLSYSIATGIACGFIFYPITMLISKKHKEVHPIMYVLMVLFILYFIFVHG"
     gene            complement(1551..3686)
                     /locus_tag="POB55_000096"
     CDS             complement(1551..3686)
                     /locus_tag="POB55_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDI6499094.1"
                     /translation="MKSLILAEKPSVARDIADALQINQKRNGYFENNQYIVTWALGHL
                     VTNATPEQYDKNLKEWRLEDLPIIPKYMKTVVIGKTSKQFKTVKALILDNKVKDIIIA
                     TDAGREGELVARLILDKVGNKKPIRRLWISSVTKKAIQQGFKNLKDGRQYNDLYYAAL
                     ARSEADWIVGINATRALTTKYDAQLSLGRVQTPTIQLVNTRQQEINQFKPQQYFTLSL
                     TVKGFDFQLESNQRYTNKETLEQMVNNLKNVDGKIKSVATKHKKSYPQSLYNLTDLQQ
                     DMYRRYKIGPKETLNTLQSLYERHKVVTYPRTDSNYLTTDMVDTMKERIQATMATTYK
                     DQARPLMSKTFSSKMSIFNNQKVSDHHAIIPTEVRPVMSDLSNRELKLYDMIVERFLE
                     ALMPPHEYDAITVTLEVAGHTFVLKENVTTVLGFKSIRQGESITEMQQPFSEGDEVKI
                     SKTNIREHETTPPEYFNEGSLLKAMENPQNFIQLKDKKYAQTLKQTGGIGTVATRADI
                     IDKLFNMNAIESRDGKIKVTSKGKQILELAPEELTSPLLTAQWEEKLLLIERGKYQAK
                     TFINEMKDFTKDVVNGIKNSDRKYKHDNLTTTECPTCGKFMIKVKTKNGQMLVCQDPS
                     CKTKKNVQRKTNARCPNCKKKLTLFGKGKEAVYRCVCGHSETQAHMDQRMKSKSSGKV
                     SRKEMKKYMNKNEGLDNNPFKDALKNLNL"
     gene            complement(3712..3807)
                     /locus_tag="POB55_000097"
     CDS             complement(3712..3807)
                     /locus_tag="POB55_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499095.1"
                     /translation="MILTCYNTFNTSFAIIEFKSKISGIDEARSK"
     gene            3873..4760
                     /locus_tag="POB55_000098"
     CDS             3873..4760
                     /locus_tag="POB55_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6499096.1"
                     /translation="MSIVQLYDITQIKSFIEHSNYESASYLYKLPQQYNEIDVLITDA
                     IESPGVFSIKENDSIKAIILSFAYDKNKFKVIGPFVADNYVLSVDTFETLFKAMTSNQ
                     PDDAVFNFSFEEGIQQYKPLMKVIQASYNFTDYYIEARTRLEEDMHQPNIIPYHKGFY
                     RAFSKLHTTTFKYQAQSPQDIIDSLDDHHHLFLFVSEGLLKGYLYLEIDSQQSIAEIK
                     YFSSHVDYRLKGIAFELLAYALQYAFDNFDIRKVYFKIRNKNNKLIERFNGLGFHINY
                     EYIKFKFESRNVKDQTIPE"
     gene            4941..5804
                     /locus_tag="POB55_000099"
     CDS             4941..5804
                     /locus_tag="POB55_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein GlcU"
                     /protein_id="HDI6499097.1"
                     /translation="MQFLDFLIALLPALFWGSVVLINVFVGGGPYNQIRGTTLGALIV
                     GLGLLITGFAKFNNPTVIIVGLISGALWAFGQANQLKSISLIGVSNTMPVSTGMQLVG
                     TTLFSVIFLGEWSSMTQIIFGLIAMILLVTGVALTSLKAKNERQSDNPEFKKAMGILI
                     VSTVGYVGFVVLGDIFGVGGTDALFFQSVGMAIGGFILSMNHKTSLKSTALNLLPGVI
                     WGIGNLFMFYSQPKVGVATSFSLSQLLVIVSTLGGIFILGERKDRRQMTGIWAGIIII
                     VIAAIILGNLK"
     gene            complement(5921..6052)
                     /locus_tag="POB55_000100"
     CDS             complement(5921..6052)
                     /locus_tag="POB55_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499098.1"
                     /translation="MYGAYLIYEYHQDFKSIANFKVLKRMINVMMCYCVLFLEPRIS"
     gene            complement(6223..7131)
                     /locus_tag="POB55_000101"
     CDS             complement(6223..7131)
                     /locus_tag="POB55_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002487080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDI6499099.1"
                     /translation="MTEQFTMIILLIALGYFLKRINFIKATDSQVIATLVLNVTLPSL
                     VIVNLNSAELKLSFSILPILMIIYGIVAKMIAVWFFRKYDNHMRGSVGMMTGAMNIGL
                     FAYPLIEVIWPKTGLIYFVMADIGGAFVMFGITYFVGSYFSEGSDQFDFKYLGKKLLQ
                     SVPLVTYIVMFILNISHIHLPHVAIDFFSILSHANMPLSMILLGVMLNFTLERKYIPA
                     TIKYLCLHYGLALVAGLLVYVLLPVSDDMIKTTLLVTWMFPVGVAIISYGIQFKYRTL
                     PFIGMTTNLTIIISIIILYVYQAVFV"
     gene            7264..7428
                     /locus_tag="POB55_000102"
     CDS             7264..7428
                     /locus_tag="POB55_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499100.1"
                     /translation="MSLENQLAELKYDYVRLQGDLEKRESLNLDTSALVRQLKDIENE
                     IRNVRAQMQD"
     gene            7673..7990
                     /gene="mspA"
                     /locus_tag="POB55_000103"
     CDS             7673..7990
                     /gene="mspA"
                     /locus_tag="POB55_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane stabilizing protein MspA"
                     /protein_id="HDI6499101.1"
                     /translation="MQFYLILLAILYLIVSFISIFKMEVVFTRILRIIMGVLLLFVLA
                     LTMMSFPKENWWVFIVLLLLVGNVEVTGFKMLKKDLKGVNILNLMSLFIFVIYFILTI
                     VLF"
     gene            complement(8174..11341)
                     /locus_tag="POB55_000104"
     CDS             complement(8174..11341)
                     /locus_tag="POB55_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HDI6499102.1"
                     /translation="MIKKLLQFSLGNKFAIFLMVVLVVLGGVYASAKLKLELLPNVQN
                     PVISVTTTMPGATPQSTQDEISSKIDNQVRSLAYVKNVKTQSIQNASIVTVEYENNTD
                     MDKAEEQLKKEIDKIKFKDEVGQPELRRNSMDAFPVLAYSFSNKENDLKKVTKVLNEQ
                     LIPKLQTVDGVQNAQLNGQTNREITLKFKQNELEKYGLTADDVENYLKTATRTTPLGL
                     FQFGDKDKSIVVDGQYQSVDAFKNINIPLTFAGGQGQSQSQSDNKQNSAMSDVNQASP
                     QQNSKASAPNNISGMPTAKLGDLADITVGDVRTSISKTNGKDAVNLQITKAQDANTVQ
                     VAKDVQRKIDTFVDENKDFNVTKTMDTAKPVEKSLYTMVEKASLGTIVAIIVILLFLR
                     NIRTTAISIISIPLSLLMALIALKLSDVSLNILTLGALTVAIGRVIDDSIVVVENIYR
                     RLTDSEEQLKGENLIISATTEVFKPIMSSTLVTIIVFLPLVFVSGSVGEMFRPFALAI
                     AFSLLASLLVSITLVPALAATLFKKGVKRRNKQHQEGLGVVSTTYKKVLHWSLNHKWI
                     VIILSTLILVVTIVFGGPRLGTSFISAGDDKFLAITYTPKPGETEQAVLNHAKDVEKY
                     LKQKKHVKTIQYSVGGSSPVDPTGSTNSMAIMVEYDNDTPNFDVEADKVIKHADGFKH
                     PGEWKNQDLGTGAGNKSVEVTVKGPSMDAIKSTVKDIEQKMKQVKGLANVKSDLSQTY
                     DQYEIKVDQNKAAENGISASQLAMHLNENLPEKTVTTVKENGKTVDVKVKQNKQTDWS
                     EDKLNNITLKKPTGGTIKLGNIATLVKTTTPSKLTQEQGDYATTVSAKVTNKDVGGTT
                     RQVMSKINNLDKPNNVKVNIGGASDDINNAMTQLAFAMLAAIIIVYLILVITFKGGLA
                     PFTILFSLPFTVIGVIIALLITGETISVPSLIGMLMLIGIVVTNAIVLIDRVINNEQQ
                     GMEMKEALIEAGGTRIRPILMTAIATIGALVPLLFGQDSSILISKGLAATVIGGLISS
                     TLLTLVVVPVIYEILFTLKKRFTKR"
     gene            complement(11458..12723)
                     /gene="femX"
                     /locus_tag="POB55_000105"
                     /gene_synonym="fmhB"
     CDS             complement(11458..12723)
                     /gene="femX"
                     /locus_tag="POB55_000105"
                     /gene_synonym="fmhB"
                     /EC_number="2.3.2.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II:glycine glycyltransferase"
                     /protein_id="HDI6499103.1"
                     /translation="MEKMHITNQEHDAFVKSHPNGDLLQLTKWAETKKLTGWYARRIA
                     VGRDGEVQGVAQLLFKKVPKLPYTLCYISRGFVVDYSNKEALNALLDSAKEIAKAEKA
                     YAIKIDPDVEVDKGTDALQNLKALGFKHKGFKEGLSKDYIQPRMTMITPIDKNDDELL
                     NSFERRNRSKVRLALKRGTTVERSDREGLKTFAELMKITGERDGFLTRDISYFENIYD
                     ALHEDGDAELFLVKLDPKENIAKVNQELNELHAEIAKWQQKMETSEKQAKKAQNMIND
                     AQNKIAKNEDLKRDLEALEKEHPEGIYLSGALLMFAGSKSYYLYGASSNEFRDFLPNH
                     HMQYTMMKYAREHGATTYDFGGTDNDPDKDSEHYGLWAFKKVWGTYLSEKIGEFDYVL
                     NQPLYQLIEQVKPRLTKAKIKISRKLKRK"
     gene            13112..13363
                     /locus_tag="POB55_000106"
     CDS             13112..13363
                     /locus_tag="POB55_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499104.1"
                     /translation="MRTIILSLFIIMNIVAIIMTLSQPLTVNYFSLRVILIFFTFILS
                     IFFILIKSSRLNNILTILSIVLAIIHMGILAHSTYVYLY"
     gene            complement(13355..14119)
                     /locus_tag="POB55_000107"
     CDS             complement(13355..14119)
                     /locus_tag="POB55_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDI6499105.1"
                     /translation="MILKFDHIIHYIDQLDRFSFPGDVIKLHSGGYHHKYGTFNKLGY
                     INENYIELLDVENNEKLKKMAKTIEGGVAFATQIVQEKYEQGFKNICLRTNNIEAVKN
                     KLQSERVEVVGPIQMERDTHKDGKVKWQLLYIMNQDDDEIKPPFFIQWEESDSMRTKK
                     LQKYFQKQFSIETVIVKSKNRSQTVSNWLKWFDMDIVEENDHYTDLILKNDDIYFRIE
                     DGKVSKYHSVIIKDAQATSPYSIFIRGAIYRFEPLV"
     gene            14281..14721
                     /locus_tag="POB55_000108"
     CDS             14281..14721
                     /locus_tag="POB55_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HDI6499106.1"
                     /translation="MLSQEFFNSFITIYRPYLKLAEPILEKHNIYYGQWLILRDIAKH
                     QPTTLIEISHRRAIEKPTARKTLKALIENDLITVENSLEDKRQKFLTLTPKGHELYEI
                     VCLDVQKLQQAVVAKTNISQDQMQETINVMNQIHKILLKETHND"
     gene            14714..15925
                     /locus_tag="POB55_000109"
     CDS             14714..15925
                     /locus_tag="POB55_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6499107.1"
                     /translation="MTNQGSLNKSPIFTKSFTINFFVNFIVYLCMYLLLVVIAGYSKQ
                     AFDASDSLAGLVVGLFIVGSLIGRFATGKFVNQIGPKRLLFIGLIALIITQLLYFIDG
                     SLAFLIFVRLINGIATAVVTTATGTIAAYVTPVNRKSEGISLFSLSLVLGTAIGPFLG
                     MLLITKYPIDLLFIICVILGILGLIISLFIKVDFEVTNNKTETNVIDKPRFSIHQFIA
                     KEAIPVAVIMLLIGVTYASILTYLQAFALERHLVTAASYFFICYAIASLITRPIAGRL
                     MDDKNENIIVYPAFIMLFLSFVCLISSYQSWLILLAGACLGLGYGNLSSAMQSIAIKV
                     SPPIKYGIATSTFYVGLDAGVGFGPSFLGLFTHMFSYSEIFGFMAALAIITMLVYFLI
                     HGRHVTRNAMN"
     gene            complement(16027..16377)
                     /gene="sarV"
                     /locus_tag="POB55_000110"
     CDS             complement(16027..16377)
                     /gene="sarV"
                     /locus_tag="POB55_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarV"
                     /protein_id="HDI6499108.1"
                     /translation="MSNKVQRFIEAERELSQLKHWLKTTHKISIEEFVVLFKVYEAEK
                     ISGKELRDTLHFEMLWDTSKIDVIIRKIYKKELISKLRSETDERQVFYFYSTSQKKLL
                     DKITKEIEVLSVTN"
     gene            16503..16673
                     /locus_tag="POB55_000111"
     CDS             16503..16673
                     /locus_tag="POB55_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499109.1"
                     /translation="MFRFISFGQLVVYSTTFIPKKDILQLNGMECDINVKEPLKIYYV
                     VILKSGIEMIFS"
     gene            complement(16955..17977)
                     /gene="moaA"
                     /locus_tag="POB55_000112"
     CDS             complement(16955..17977)
                     /gene="moaA"
                     /locus_tag="POB55_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HDI6499110.1"
                     /translation="MVEQIKDKLGRPIRDLRLSVTDRCNFRCDYCMPKEVFGDDFVFL
                     PKNELLTFDEMARIAKVYAELGVKKIRITGGEPLMRRDLDVLIAKLNQIDGIEDIGLT
                     TNGLLLKKHGQKLYDAGLRRINVSLDAIDDTLFQSINNRNIKATTILEQIDYATSIGL
                     NVKVNVVIQKGINDDQIIPMLEYFKDKHIEIRFIEFMDVGNDNGWDFSKVVTKDEMLT
                     MIEQHFEIDPVEPKYFGEVAKYYRHKDNGVQFGLITSVSQSFCSTCTRARLSSDGKFY
                     GCLFATVDGFNVKAFIRSGVTDEELKEQFKALWQIRDDRYSDERTAQTVANRQRKKIN
                     MNYIGG"
     gene            complement(17997..18596)
                     /gene="mobA"
                     /locus_tag="POB55_000113"
     CDS             complement(17997..18596)
                     /gene="mobA"
                     /locus_tag="POB55_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HDI6499111.1"
                     /translation="MKAIILAGGHSVRFGKPKAFAEVNGETFYSRVIKTLESTNMFNE
                     IIISTNAQLATQFKYPNVVIDDENHNDKGPLAGIYTIMKQHPEEELFFVVSVDTPMIT
                     GKAVSTLYQFLVSHLIENHLDVAAFKEDGRFIPTIAFYSPNALGAITKALHSDNYSFK
                     NVYHELSTDYLDVRDVDAPSYWYKNINYQHDLDALIQKL"
     gene            complement(18603..18836)
                     /gene="moaD"
                     /locus_tag="POB55_000114"
     CDS             complement(18603..18836)
                     /gene="moaD"
                     /locus_tag="POB55_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HDI6499112.1"
                     /translation="MKVLYFAEIKDILQKAQEDIVLEQALTVQQFEDLLFERYPQINN
                     KKFQVAVNEEFVQKSDFIQPNDTVALIPPVSGG"
     gene            complement(18842..19288)
                     /locus_tag="POB55_000115"
     CDS             complement(18842..19288)
                     /locus_tag="POB55_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HDI6499113.1"
                     /translation="MKQFEIVTEPIQTEQYREFTINEYQGAVVVFTGHVREWTKGVKT
                     EYLEYEAYIPMAEKKLAQIGDEINEKWPGTITSIVHRIGPLQISDIAVLIAVSSPHRK
                     DAYRANEYAIERIKEIVPIWKKEIWEDGSKWQGHQKGNYEEAKREE"
     gene            complement(19302..19787)
                     /gene="mobB"
                     /locus_tag="POB55_000116"
     CDS             complement(19302..19787)
                     /gene="mobB"
                     /locus_tag="POB55_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HDI6499114.1"
                     /translation="MILQIVGYKKSGKTTLMRHIVSFLKSHGYTVATIKHHGHGKEDI
                     QLQDSDVDHMKHFEAGADQSIVQGFQYQQTVTRVDNQNLTQIIEKSVTIDTNIVLVEG
                     FKNADFEKVVVYRNEEELQVLQQLSNVCYSINVREHEDFTAFEQWLLNKIKNDCDTQL
                     T"
     gene            complement(19784..21043)
                     /locus_tag="POB55_000117"
     CDS             complement(19784..21043)
                     /locus_tag="POB55_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HDI6499115.1"
                     /translation="MVVEKRNPIPVKEAIQRIVNQQSSMPAITVALEKSLNHILAEDI
                     VATYDIPRFDKSPYDGFAIRSVDSQGASGQNRIEFKVIDHIGAGSVSDKLVGDHEAVR
                     IMTGAQIPNGADAVVMFEQTIELEDTFTIRKPFSKNENISLKGEETTTGDVVLKKGQV
                     INPGAIAVLATYGYAEVKVIKQPSVAVIATGSELLDVNDVLEDGKIRNSNGPMIRALA
                     EKLGLEVGIYKTQKDDLDSGIQVVKEAMEKHDIVITTGGVSVGDFDYLPEIYKAVKAE
                     VLFNKVAMRPGSVTTVAFVDGKYLFGLSGNPSACFTGFELFVKPAVKHMCGALEVFPQ
                     IIKATLMEDFTKANPFTRFIRAKATLTSAGATVVPSGFNKSGAVVAIAHANCMVMLPG
                     GSRGFKAGHTVDIILTESDAAEEELLL"
     gene            21106..21600
                     /gene="moaC"
                     /locus_tag="POB55_000118"
     CDS             21106..21600
                     /gene="moaC"
                     /locus_tag="POB55_000118"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HDI6499116.1"
                     /translation="MTEFTHINQQGHAKMVDVSDKQITKRTAVAHSSITVNKTIFKQI
                     SNNTNTKGNVLNTAQIAGIMAAKNTSTIIPMCHPLPLTGIDVHFSWDETNAPLYTLNI
                     QTTVSTTGKTGVEMEALTAASATALTIYDMTKAVDKGMIIGETYLESKSGGKSGDFQR
                     QSNQ"
     gene            complement(21602..22108)
                     /locus_tag="POB55_000119"
     CDS             complement(21602..22108)
                     /locus_tag="POB55_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaB"
                     /protein_id="HDI6499117.1"
                     /translation="MGEHQNVKLNRTVKAAVLTVSDTRDFDTDKGGQCVRQLLQADDV
                     EVSDAHYTIVKDEKVAITTQVKKWLEEDIDVIITTGGTGIAQRDVTIEAVKPLLTKEI
                     EGFGELFRYLSYVEDVGTRALLSRAVAGTVNNKLIFSIPGSTGAVKLALEKLIKPELN
                     HLIHELTK"
     gene            complement(22139..23143)
                     /locus_tag="POB55_000120"
     CDS             complement(22139..23143)
                     /locus_tag="POB55_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HDI6499118.1"
                     /translation="MSQERYSRQILFKQIGEIGQSKINQKCALIIGMGALGTHVAEGL
                     VRAGIAKLIIVDRDYIEFSNLQRQTLFTEEDALKMMPKVVAAKKHLLALRSDVDIDDY
                     IAHVDYYFLETHGQDVDVIIDATDNFETRQLIDDFAYKHRIPWIYGGVVQSTYTEAAF
                     IPGKTPCFNCLVPQLPALNLTCDTVGVIQPAVTMATSLQLRDAMKVLTEQPIDTKITY
                     GDIWDGSHYSFGFSKMQRSDCTTCGDVPSYPYLNKNEQRYATLCGRDTVQYENASITH
                     DILVQFLKQHQLNYRSNSYMVMFEFKGHRIVAFKGGRFLIHGMTRTSDATHLMNLLFG
                     "
     gene            complement(23313..23918)
                     /locus_tag="POB55_000121"
     CDS             complement(23313..23918)
                     /locus_tag="POB55_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDI6499119.1"
                     /translation="MLKINVKYQLKNTLIRINIDDTEPKIYAVRGPSGIGKTTVLNMI
                     AGLRKADEAIIEVNGQLLTDTAKNVNVKIQQRRIGYLFQDYQLFPNMTVYKNITFMAE
                     PSEHIDQLIQTLNIDHLMKQYPMTLSGGEAQRVALARALSTKPDLILLDEPFSSLDDT
                     TKDESITLVKRIFNEWQIPIIFVTHSNYEAEQMAHEIITIG"
     gene            complement(23919..24590)
                     /gene="modB"
                     /locus_tag="POB55_000122"
     CDS             complement(23919..24590)
                     /gene="modB"
                     /locus_tag="POB55_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HDI6499120.1"
                     /translation="MPDLTPFWISIRVAVISTIIVTVLGIFISKWLYHRKGSWVKVLE
                     SLLILPIVLPPTVLGFILLIIFSPRGPIGQFFANVLHLPVVFTLTGAVIASVIVSFPL
                     MYQHTVQGFRGIDTKMINTARTMGASETKIFLKLILPLAKRSILAGIMMSFARALGEF
                     GATLMVAGYIPNKTNTLPLEIYFLVEQGRENEAWLWVLVLVAFSIVVISTINLLNKDK
                     YKEVD"
     gene            complement(24604..25386)
                     /gene="modA"
                     /locus_tag="POB55_000123"
     CDS             complement(24604..25386)
                     /gene="modA"
                     /locus_tag="POB55_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDI6499121.1"
                     /translation="MKMKRFIAIVMALFLVLAGCSNSNDNNESKKDDADNGKKQEIQV
                     AAAASLTDVTKKLASEFKKEHKNADIKFNYGGSGALRKQIESGAPVDVFMSANTKDVD
                     ALKDKNKAHDTYKYAKNSLVLIGDKDSNYTSVKDLKDNDKLALGEVKTVPAGKYAKQY
                     LDNNNLFKEVESKIVYAKDVKQVLNYVEKGNAKQGFVYKTDLYKQNKKIDTVKVIKEV
                     ELKKPITYEAGATSDSKLAKEWMEFLKSDKAKEILKEYHFAA"
     gene            25676..26473
                     /gene="fdhD"
                     /locus_tag="POB55_000124"
     CDS             25676..26473
                     /gene="fdhD"
                     /locus_tag="POB55_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HDI6499122.1"
                     /translation="MNKDVSLGQPIVRYEDGKLFNTTDQYVTEFPLTIMVNGEEFATV
                     ICSPTNLEELVIGFLASEGAILKRDELKSVLIDDSKGFAHVELNKDLGDRFQYSTKRM
                     IASCCGKSREFYFQNDAAIAKTSMSKITLTPMQIINMMTRLQSASHIYQETGGLHNAA
                     ISDGLTFFVHRQDIGRHNALDKLYGFCIQRHITVRDKVLIFSGRISSEILIKAAKIGV
                     GVILSKSAPTTLAVTLANDLNITAVGFIRNGGFNIYSHPERIIDSEQ"
     gene            complement(26645..27415)
                     /locus_tag="POB55_000125"
     CDS             complement(26645..27415)
                     /locus_tag="POB55_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6499123.1"
                     /translation="MASISMSDIYCNGTIFENDDEQLIYLTPSFPQRYTSNTWIYKKT
                     PTQERWLKDLERQHQLHTNQGSNHYAFSFPENEQLDNHWMAMFKDMNFELGIMELYAI
                     ESDALANLPRNSDVEIAIVDESHIDAYLKVAYQFSLPFGKDYADAHEEMVREHYQKDV
                     IKRLVAYLNNEPIGVVDVIESENYIELDGFGVLEQFRHQGIGSTIQSLIAEYAISKNH
                     KPIILVADGEDTAKDMYAKQGYVYQSFCYQILKEDIGN"
     gene            complement(27492..28046)
                     /locus_tag="POB55_000126"
     CDS             complement(27492..28046)
                     /locus_tag="POB55_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HDI6499124.1"
                     /translation="MTTKQLVYTALMTAIIAILGLVPVIPLPFSSVPIVLQNIGIFLA
                     GVILGRKYGTLSVIVFLLLVVAGLPLLSGGRGGIGVFAGPSAGFLLLYPVVAFMIGAI
                     RDRFINEINFWILFVGILVFGVIALDVIGTLIMGMIINIPFTKAISISLAYLPGDILK
                     AIVASLIGTALLNHSQFRQIMGLK"
     gene            complement(28167..29108)
                     /locus_tag="POB55_000127"
     CDS             complement(28167..29108)
                     /locus_tag="POB55_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HDI6499125.1"
                     /translation="MTKKVYFNHDGGVDDLVSLFLLLQMENVQLIGVSTIGADCYLEP
                     SLSASVKIINRFSNEDIQVAPSYERGKNPFPKEWRMHAFFMDALPILNEPVKHVASNV
                     SDKEAFEDIIQTLKRQSEKVTLLFTGPLTDLAKALQKDSSIVQYIEKLVWMGGTFLPK
                     GNVEEPEHDGSAEWNAYWDPEAVKIVFDSDIEIDMVALESTNQVPLTLDVRQRWANER
                     QYTGIDFLGVSYAAVPPLTHFITNSTYFLWDVLTTAYIGNKDLVHSIEKKVDVISYGP
                     SQGKTFECKDGRKINVINHVDNNAFFDYITALAKKVN"
     gene            complement(29506..30414)
                     /locus_tag="POB55_000128"
     CDS             complement(29506..30414)
                     /locus_tag="POB55_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDI6499126.1"
                     /translation="MKKLLLPLIIMLLVLAACGNQGEKNNKAETKSYKMDDGKTVDIP
                     KDPKRIAVVAPTYAGGLKKLGANIVAVNQQVDQSKVLKDKFKGVTKIGDGDVEKVAKE
                     KPDLIIVYSTDKDIKKYQKVAPTVVVDYNKHKYLEQQEMLGKIVGKEDKVKAWKKDWE
                     ETTAKDDKEIKKAIGQDATVSLFDEFDKKLYTYGDNWGRGGEVLYQAFGLKMQPEQQK
                     LTAKAGWAEVKQEEIEKYAGDYIVSTSEGKPTPGYESTNMWKNLKATKEGHIVKVDAG
                     TYWYNDPYTLDFMRKDLKEKLIKAAK"
     gene            complement(30628..31782)
                     /locus_tag="POB55_000129"
     CDS             complement(30628..31782)
                     /locus_tag="POB55_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HDI6499127.1"
                     /translation="MNDMDNSFLITTEIQRKWIEKFKVIRDTFKAKAEYNDQHSQFPY
                     KNIEWLIKEGYGKLTLPKAYGGEGATIEDMVILQSFLGELDGATALSIGWHVSVVGQI
                     YEQKLWSQDMLEQFAVEINNGALVNRAVSEAEMGSPTRGGRPSTHAVKADDGYILNGV
                     KTYTSMSKALTHIIVAAYIEELEGVGFFLVDRNLPGVEIADNWDVLGMRATESHDLIL
                     NDVKVPLKHLVETEKSKAPNGWILHIPSCYLGIAQAARNYAVDFAIQHSPNSIEGTIA
                     TLPTVQQNLGKMETLLLCARQFLWSTAKGYQQYKDDSQIRNSTSASKVMVMNQGLEVI
                     DLAMRIVGAKSLEMNRPLQRYYRDMRAGLHNPPMEDAAYTNIAKSITDTF"
     gene            complement(31999..32904)
                     /gene="yut"
                     /locus_tag="POB55_000130"
     CDS             complement(31999..32904)
                     /gene="yut"
                     /locus_tag="POB55_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016266007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HDI6499128.1"
                     /translation="MKIIDVLLKNISQVVLISNKWTGLFILIGLFVADWTIGLAAIVG
                     SIIAYAFARYINYSEAEINDGLAGFNPVLTAIALTIFLDKSGLDIVITMIATLLTLPV
                     AAAVREVLRPYKVPMLTMPFVIVTWFTILLSGQVKFVDTSLKLMPQNIETVNFSNNDR
                     IHFIQSLFEGFSQVFIEASVIGGVCILIGILIASRKAALLAVIASLLSFIIVALLGGN
                     YDDINQGLFGYNFVLMAIALGYTFKTAINPYISTFLGVLLTVVVQLGTTTLLEPFGLP
                     ALTLPFIIVTWILLFAGIKHDKVDA"
     gene            33164..33466
                     /locus_tag="POB55_000131"
     CDS             33164..33466
                     /locus_tag="POB55_000131"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HDI6499129.1"
                     /translation="MHFTQREQDKLMIVVAAEVARRRKARGLKLNHPEALALISDELL
                     EGARDGKTVAELMSYGRQILNKEDVMDGVEHMITDIEIEATFPDGTKLITVHHPIV"
     gene            33480..33890
                     /locus_tag="POB55_000132"
     CDS             33480..33890
                     /locus_tag="POB55_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HDI6499130.1"
                     /translation="MIPGEIITKSTEVEINNHHPETVIEVENTGDRPIQVGSHFHFYE
                     ANAALDFEREMAYGKHLDIPAGAAVRFEPGDKKEVQLVEYAGKRKIFGFRGMVNGPID
                     ESRVYRPTDENDEYAGVFGDNGAENVNKKGGKRS"
     gene            33887..35602
                     /gene="ureC"
                     /locus_tag="POB55_000133"
     CDS             33887..35602
                     /gene="ureC"
                     /locus_tag="POB55_000133"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002447295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HDI6499131.1"
                     /translation="MSFKMTQNQYTSLYGPTVGDSIRLGDTNLFAQIEKDYAVYGEEA
                     TFGGGKSIRDGMAQNPRVTRDDVNVADLVISNAVIIDYDKVVKADIGIKNGYIFAIGN
                     AGNPDIMDNVDIIIGSTTDIIAAEGKIVTAGGIDTHVHFINPEQAEVALESGITTHIG
                     GGTGASEGSKATTVTPGPWHIHRMLEAAEGLPINVGFTGKGQATNPTALIEQINAGAI
                     GLKVHEDWGATPSALSHALDVADEFDVQIALHADTLNEAGFMEDTMAAVKDRVLHMYH
                     TEGAGGGHAPDLIKSAAFSNILPSSTNPTLPYTHNTVDEHLDMVMITHHLNAAIPEDI
                     AFADSRIRKETIAAEDVLQDMGVFSMISSDSQAMGRVGEVITRTWQVAHRMKEQRGPL
                     DGDFEHNDNNRIKRYIAKYTINPAITHGISEYVGSIEPGKLADIVLWDPIFFGVKPEL
                     VVKGGLINSAVNGDANGSIPTSEPMKYRKMYGQYGGNLTSTSMTFVSKTAYENGINRA
                     LNLKRMVRPVKNIRQLSKADMKNNSATPKLDVDPQTYEVYVDGEKITSNAATELPLTQ
                     RYFLF"
     gene            35615..36067
                     /gene="ureE"
                     /locus_tag="POB55_000134"
     CDS             35615..36067
                     /gene="ureE"
                     /locus_tag="POB55_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HDI6499132.1"
                     /translation="MIVEEIQGNIANLSNSEKQKHVEKVYLENSDLVKRIQRVVTDHG
                     TEIGIRLKQPIDLQYGDILYADDHNMIIVDVNSEDLLVIQPRTLQEMGDIAHQLGNRH
                     LPAQFTETEMLVQYDYLVEDLLKSLGIPYVREDRKVNKAFRHIGHSHD"
     gene            36060..36749
                     /locus_tag="POB55_000135"
     CDS             36060..36749
                     /locus_tag="POB55_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HDI6499133.1"
                     /translation="MIDHTHLRLFQFCDSQFPTGAFSHSFGLETYIQRNIIHDDHTFI
                     AWLKMFLQEQLTYSDGLAMRLVYDALENDDTQKVLHIDKLMFVQNLPKETRVGAKQMG
                     TRMVKLALELYNSPWIAWYHQQMQDKKAKLNPAICFTMLGHHLGVDIETIIDYYLYQN
                     VSSLTQNAVRAIPLGQTAGQKIVTHMIPYIEGTRKQIFELKEADFGMTAPGLELNQMA
                     HENVNVRIFIS"
     gene            36762..37376
                     /gene="ureG"
                     /locus_tag="POB55_000136"
     CDS             36762..37376
                     /gene="ureG"
                     /locus_tag="POB55_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HDI6499134.1"
                     /translation="MANPIKIGIGGPVGAGKTQLIEKVVKRLSKEMSIGVITNDIYTK
                     EDEKILVNSGVLPESRIIGVETGGCPHTAIREDASMNFAAIDELLERHDDIELIFIES
                     GGDNLAATFSPELVDFSIYIIDVAQGEKIPRKGGQGMIKSDFFIINKTDLAPYVGASL
                     EQMAEDTKVFRGKRPFTFTNLKTDEGLDEVIDWIERDTLLKGLS"
     gene            37376..38212
                     /locus_tag="POB55_000137"
     CDS             37376..38212
                     /locus_tag="POB55_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HDI6499135.1"
                     /translation="MDEQQWTGQLDLTVFFDGNRSVSRDIFFEKALKVIRPVYLNQST
                     IPTFYIVNVGGGYLDGDRYRMNVNVEDNAKVTLTSQGATKIYKTPSNHVEQYQTFNLK
                     DNAYLEYVADPIIAYENAKFYQHNTFNLNNSSSLFYTDILTPGYSKTGEAFKYQYMHL
                     INEIYIEDELVTYDNLLLNPNKQSINEIGYMEHYSHYGSAYFIHEDVNQKLIDSVYET
                     ISSYSNTFDCRVAISQLPTHGFAVRIFAYRTQIIEKILGIIQSYIAENIYDRKLDFLR
                     KY"
     gene            complement(38401..38748)
                     /gene="sarR"
                     /locus_tag="POB55_000138"
     CDS             complement(38401..38748)
                     /gene="sarR"
                     /locus_tag="POB55_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarR"
                     /protein_id="HDI6499136.1"
                     /translation="MSKINDINDLVNATFQVKKFFRDTKKKFNLNYEEIYILNHILRS
                     ESNEISSKEIAKCSEFKPYYLTKALQKLKDLKLLSKKRSLQDERTVIVYVTDTQKANI
                     QKLISELEEYIKN"
BASE COUNT        12384 a         7132 c         5694 g        13719 t
ORIGIN      
        1 agacattcat aatattgctt tatgttattt cgcttcttat tttatcctta cttcaaagaa
       61 aaaacgacta ccaatcattg gcagtcgttt tttgtgtctg gatttaacca tgaacaaata
      121 tgaagtacag aatgaatagt accattaata catacattat cggatgtact tctttatgtt
      181 ttttagagat aagcattgta attggataga aaataaatcc acacgcaata ccagttgcaa
      241 ttgagtatga taaaggcatc ataatgattg taatgaatgc tggcactgca acttcaaaac
      301 ttttccagtt tatctcagca aagtttgcag ccattaatac acctaccacc actaaagctg
      361 gtgtcgtaac tgcacttgtt acaacagcga ttaacggact aaagaacagc gctaatagga
      421 aacaacaacc tgttacgata ctagcaaatc ctgttctagc acctacagca acacctgaag
      481 ttgattcgat ataagaagtt gttgtagttg ttccaaaaac tgatcctaca atcgtagcta
      541 aagaatctga gaacaatgct ctaccagctc ttggtaattt attatctttc ataatacctg
      601 cttgagtagc aacagctact aatgtaccag ctgtatcaaa gaaatcaata aataagaacg
      661 ttaaaataac aattaagaac tgtatcgtta ataattgact tgggtcttta aacgcttcaa
      721 atgcagcacc gaacgttggt tcaatacttg gaatcttacc tacaatacca gatggcattt
      781 gaatcaatcc agtgaacata ccaacgattg ctgtgatgat cataccgata aagatagaac
      841 ccggcaattt aattgcatat aaaataactg taataacgat accgaatatc gccaaaagta
      901 ctggtggatc agttaaatgt cccaatgtta ctaatgtaga ttcattttta actataatac
      961 ccgaactttg taaacctaca aatgtaatga ataatcctat acctgctgaa acagccatct
     1021 tcatttgata aggaattgca ttaataataa cttccctaaa ccctgtgacg gttaatatcg
     1081 caaagaatat acctgagaat aaaacgcctg ttaaaccaac ttgccaagga atacccatgg
     1141 ttaacacaac agtaaatgca aagaatgcat ttaatcccat acctggtgct aacgcaattg
     1201 gatatttagc tattagcccc atgaatagcg agcctacaaa tgctgctaat gcagtcgcta
     1261 caaaaatggc accttggtcc attttcatat cttctgatac gcctttaaca cctgctaaac
     1321 ttaaaacttg cgggttaact gctaaaatat aggccataga taagaaagtt gtgataccgc
     1381 ctaagatttc tcttttaaag tttgttccat atttatcgaa ctggaaatag tttttcacga
     1441 atggatgctc ctttttttaa aataccttaa tagtttaata tcattcttaa taaaaaacaa
     1501 taccaaaagc gaacttttcg tttttctgat tcaactttgt tcgattttat ctataaattc
     1561 aagttcttta atgcatcttt aaacggatta ttgtctaaac cttcattttt attcatatac
     1621 tttttcattt ctttacgaga tactttacca gaggatttag acttcatacg ctgatccata
     1681 tgtgcttgcg tttcagaatg tccacaaaca caacgatata ccgcttcttt ccctttacca
     1741 aacaacgtta atttcttttt acagtttgga catcttgcat ttgttttgcg ctgtacattc
     1801 tttttcgtct tacaagatgg atcttggcac acaagcatct gaccattttt agttttaact
     1861 ttaatcatga atttaccaca cgttgggcat tctgtggttg ttaaattatc gtgtttatat
     1921 ttacgatcac tatttttaat cccatttaca acatctttcg taaaatcttt catttcatta
     1981 atgaatgttt tcgcctgata tttaccacgt tcaattaaaa gtaatttttc ttcccattgt
     2041 gcagttaaaa gtggcgacgt taattcttct ggtgctaatt ctaatatttg tttacctttt
     2101 gacgttactt taattttacc gtctcttgat tcaatggcat tcatattaaa taatttatcg
     2161 ataatgtcgg cccttgttgc aactgtgccg ataccacctg tttgttttaa agtttgcgca
     2221 tattttttat ccttcaattg aataaagttc tgagggttct ccatcgcttt taataacgaa
     2281 ccttcattaa aatattctgg aggtgttgtt tcatgttctc taatatttgt ttttgaaatc
     2341 ttcacttcat cgccttctga aaaaggctgt tgcatctctg taatagattc accttgtcta
     2401 atagatttaa aacctaaaac agttgttaca ttctctttca aaacaaatgt gtgccctgca
     2461 acctctaaag ttacagttat cgcgtcatac tcgtgcggag gcattaaagc ttctaaaaaa
     2521 cgctcgacaa tcatatcgta taactttaat tctctattac ttaagtctga catgacaggt
     2581 ctcacttctg taggaataat tgcatggtga tcagatactt tttgattatt aaatatcgac
     2641 atttttgatg aaaatgtttt agacattaat gggcgtgctt ggtctttata tgttgttgcc
     2701 atcgtcgcct gaatacgttc tttcatagta tctaccatat cagttgttaa atagtttgaa
     2761 tctgttcttg gataggttac gactttatgt ctctcatata agctttgaag tgtattcaat
     2821 gtttctttag gtccaatttt ataacgtcta tacatatctt gttgtaaatc tgttaaattg
     2881 tacagtgatt gcggatacga cttcttatgt ttagtagcaa cagatttaat cttaccatcg
     2941 acatttttca aattattaac catctgttct aaagtttctt tattggtata tcgctgattt
     3001 gattctagct gaaaatcaaa cccttttacc gttaatgata atgtaaagta ttgttgtggt
     3061 ttgaactgat taatctcttg ttgtcgtgta tttactaatt gaatcgttgg tgtctgaaca
     3121 cgtcccaggg atagctgtgc atcatacttt gttgttagtg cacgcgttgc attaatccca
     3181 acaatccaat ctgcctcact tctcgctaac gctgcataat acaaatcgtt atattgacga
     3241 ccgtctttta aatttttaaa accttgttga atagcttttt tagtaactga gctaatccat
     3301 aatcgacgga ttggcttttt gttaccaact ttatccaaaa tcaatcttgc aactagttca
     3361 ccttctcgtc cagcatctgt tgcaataata atatctttca ctttattatc taaaattaac
     3421 gcttttactg ttttaaattg tttgcttgtt ttaccaataa caacagtttt catatattta
     3481 ggtataattg gaaggtcttc taatcgccat tcctttaaat ttttatcgta ttgttcaggt
     3541 gtcgcatttg tcactagatg acctaacgcc cacgtgacaa tatattggtt attttcaaag
     3601 taaccattac gcttctgatt tatttgtaaa gcatcagcaa tatctcttgc gactgatggt
     3661 ttttcagcta atattaaaga tttcataaat tatcctttct catacgttct tttatttcga
     3721 acgtgcttca tctattccac taatctttga tttaaattca atgattgcaa atgatgtgtt
     3781 aaatgtattg taacatgtta atatcactat taactttcat ttcagttgaa atactatata
     3841 ataaaagtaa caaaaagtac ggaggtaatg acatgagcat agttcagtta tatgatatta
     3901 cacaaataaa atcgttcatt gaacattcga attatgaatc agcatcatac ttatataaac
     3961 ttcctcaaca gtacaatgaa atagatgtat taataaccga tgcgattgaa tcacctggtg
     4021 tattttcgat taaagaaaac gattcaatca aagcaatcat attgtctttt gcatacgata
     4081 aaaataaatt caaagtcata ggccctttcg tggctgacaa ttatgtatta tctgtcgata
     4141 cgtttgaaac gctatttaaa gcaatgactt cgaaccaacc tgacgatgcc gtctttaact
     4201 tttcttttga agaaggcatt caacaataca aaccattaat gaaagttatt caagcaagtt
     4261 ataacttcac tgactattac atagaagccc gtacaagatt agaagaagat atgcaccaac
     4321 caaatatcat tccttatcac aaagggtttt atcgtgcttt cagcaaatta cacacaacta
     4381 catttaaata tcaggcacag tcaccacaag atatcattga tagtttagac gaccatcatc
     4441 atttgttttt atttgttagc gaaggtttac ttaaaggtta tttatacctt gaaattgatt
     4501 cacaacagtc aatcgccgag attaaatact tcagttctca tgtagattac cgtttgaaag
     4561 gtatcgcttt cgagttgctt gcgtatgcat tgcaatatgc ttttgataat tttgatatta
     4621 gaaaagttta ttttaaaatt cgtaataaaa ataataaact catcgaacga tttaatggtc
     4681 taggtttcca tatcaactat gagtacatta aattcaaatt cgaatcacgt aacgtaaaag
     4741 atcaaacaat ccctgaataa aacaccaagc aaatacccta cagtacatca ttagcatgta
     4801 ttgtgggttt ttctactttt tgtaaatatt gaaaattata agtagttgtt ttttactatt
     4861 agggcagaat gctttacaat aacatgcaag tgtcaattaa ggggagcact tgcataaata
     4921 gtataggaga gtgagtagtc ttgcaatttc ttgatttctt aatcgcactt ttacctgctt
     4981 tattctgggg aagtgtcgtt cttattaatg tgttcgtcgg cggtggacct tacaaccaaa
     5041 ttcgtggtac gacgctaggc gcactcattg ttggattagg tttacttatt actggtttcg
     5101 caaaattcaa taatcctact gtaattattg tcggtcttat ttctggtgca ttatgggcgt
     5161 ttggacaagc gaatcagctt aaatctatta gtttaatcgg tgtatcaaat actatgccag
     5221 tttctacagg tatgcaatta gttggtacaa cattattcag cgttatcttt ttaggtgaat
     5281 ggtcttcaat gactcaaatt atctttggtt taatcgccat gatattatta gttactggtg
     5341 tagcacttac ttcacttaaa gctaaaaatg aacgtcaatc agataatcct gaatttaaaa
     5401 aagcaatggg tattttaatt gtatctacag ttggatatgt aggtttcgtt gtacttggtg
     5461 acatctttgg tgttggtgga actgatgcat tgttcttcca atctgtcggt atggcaattg
     5521 gtggctttat cctatccatg aatcataaaa catcacttaa atcaacagca cttaatctat
     5581 tgccaggtgt gatttgggga attggtaact tgttcatgtt ctattctcaa ccaaaagttg
     5641 gtgtagctac aagtttctca ttatcacagt tacttgttat cgtttcaacc ttaggcggta
     5701 ttttcatttt aggagaaaga aaagatcgtc gtcagatgac gggtatttgg gcaggtatta
     5761 ttattatcgt gatagctgct ataattctag gtaatttgaa atagaaagtt aaatactcat
     5821 gtaacgtaaa aatgtaatca cttctgaaaa taaccattca cttatagaat gattaaaatt
     5881 aattttcggg aattttacgt tgaatgttcc tctatatgtc ctaggaaata cgtggctcta
     5941 aaaacaaaac gcaataacac atcatgacat taatcatgcg ttttaagact ttaaaattag
     6001 cgatactttt aaaatcttga tgatattcat atatcaagta tgcgccatac atatgaagtg
     6061 gatagctgca taacgcactg cattatcaac ttgaatgtat gagttgaaca actatgtcat
     6121 aaataaaagc ccccttttca caatatacat ttacatattg tggtaaaggg ggctctcatt
     6181 ttctacgaat actaaaatgg attttatttt caaatgtgta aactagacaa acactgcctg
     6241 atacacgtac aaaataatga tactaataat gattgtcaaa ttggtcgtca tacctataaa
     6301 tggcagtgtt cgatatttaa actgaatacc ataagaaata attgcaacac ctaccgggaa
     6361 catccaagtg accaacaatg tcgtcttaat catatcatct gatactggta acaacacata
     6421 tactaacaat cccgcaacta atgctaatcc ataatgcaaa cataaatatt taatagtagc
     6481 aggtatatac tttctttcca gagtaaaatt caacatgaca cctagcaaaa tcattgataa
     6541 cggcatattt gcatgggaaa gtatgctaaa gaaatcgatt gccacatgtg gtaaatggat
     6601 gtgacttata ttcaatataa acattacaat gtatgtaacg agtggcactg attgtaataa
     6661 tttcttacct aaatatttaa aatcgaattg atcactacct tcactaaagt agctacctac
     6721 aaagtaagta attccaaaca tcacaaaggc accacctata tcagccataa caaaataaat
     6781 aagtcccgtt ttaggccata tcacttcaat tagtggatat gcaaacaatc caatattcat
     6841 agcaccagtc atcataccta ctgatccacg catgtgatta tcatattttc taaaaaacca
     6901 gacagcaatc attttcgcca caataccata tataatcatt aaaattggta aaatggagaa
     6961 tgacaatttt aattctgcac tgtttaaatt cacaataact aaagatggga gtgtgacatt
     7021 aagaactaat gtagcaatga cttgactatc tgttgctttt ataaaattaa tgcgcttcaa
     7081 aaagtaacca agcgcaatta ataaaataat catagtaaat tgttctgtca ctgttatccc
     7141 ttctttcaat aatcttcata atttataact ttaacatact ccacagatat tttagaagtc
     7201 tactgtttca tgctataatc tacattaaat gcacttaatt atatttcaaa ggagtgttat
     7261 agtatgtctt tagaaaacca actagccgaa cttaaatatg attatgttcg tcttcaaggt
     7321 gacttagaaa aacgggaatc tttgaattta gatacttccg cacttgttcg tcaacttaaa
     7381 gatattgaaa atgaaattag aaacgttcgc gctcaaatgc aagattaata atctatcatt
     7441 caagcaataa atgcttttcg ttacataaat ttgactagca ttgctctgaa tacgttatat
     7501 tgatgaattg ctttattttt cgctcaatta catctagaat cacaagatgt tgtcgtgtta
     7561 tgatttagtg tttcattaac aacatacacg catatctatc ccaacactgc tatttatgtt
     7621 ttctacgctg ctgtactaca tgaacccttt gaaacggaga ggaagttatc atatgcaatt
     7681 ttatctgatt ttactagcaa tactttatct aattgttagt tttattagta tttttaaaat
     7741 ggaagttgta tttactcgca ttttgagaat tattatgggt gtgttgttat tattcgtctt
     7801 agcattaacg atgatgagtt ttccaaaaga gaattggtgg gtatttatcg tcttattact
     7861 cttagtcggt aatgtcgaag tgacaggatt taaaatgctt aaaaaagatc taaaaggtgt
     7921 aaacatcttg aatttaatgt cattatttat ctttgtcata tatttcatct taaccatcgt
     7981 attattctaa gtaataaaca aaacatgcaa taacataatt gcaattttat tttattatcg
     8041 tacatgttga atgccagcat tgttttatta acacatcgat taaatcgcac gtgaaataag
     8101 atctaaaatt tcaaatttta aaaagccata aaagtagtca atatcatgac cgcttttatg
     8161 gcttttatat tatctatcgt ttagtgaatc gttttttcaa agtaaagaga atttcatata
     8221 ttactggtac aactacaagt gttaataaag ttgatgaaat taaaccacca ataactgtag
     8281 cggctaatcc tttcgaaata agaatcgagc tatcttgacc aaataacaaa ggaactaatg
     8341 caccgattgt tgcaattgcc gtcattaata tcggtctaat tctagtaccg cctgcttcga
     8401 ttaatgcttc tttcatctcc atgccctgct gctcattatt aatgacacgg tctattaaca
     8461 caatggcatt tgttactacg attccaatta acattagcat accaattaaa cttggtactg
     8521 atattgtttc tcctgtgatt aatagtgcaa taattacacc gataactgta aatggtaaag
     8581 agaatagaat tgtaaatggt gctaagccac ctttaaatgt aataactagg attaaatata
     8641 cgataatgat cgcagctaac attgcaaagg ctaattgtgt cattgcattg ttaatatcat
     8701 ctgatgcacc accgatatta acctttacat tattcggttt gtccaaatta tttattttag
     8761 acatcacttg tcgtgttgtg ccacccacat ctttatttgt tactttagca gataccgtcg
     8821 ttgcataatc tccttgttct tgcgtcaatt tacttggtgt cgttgtttta actaacgtag
     8881 cgatatttcc caatttaatc gtaccaccag tcggcttttt caaagttata ttattcaact
     8941 tatcttctga ccagtctgtt tgcttatttt gtttgacttt aacatcaaca gttttaccat
     9001 tttctttaac agtcgtaact gttttttctg gtaagttttc attcaagtgc attgcaagtt
     9061 gacttgcaga aataccattt tctgccgctt tattttgatc aactttaatt tcatactggt
     9121 catatgtttg cgataaatca gatttgacat tggctagtcc tttaacctgt ttcattttct
     9181 gttcaatatc ttttacagtt gattttatgg catccattga tggacctttt acagtaacct
     9241 ctacagattt attacctgca cctgttccta aatcttgatt tttccactct ccaggatgtt
     9301 taaagccatc tgcatgttta ataaccttat ccgcttctac atcaaaatta ggcgtgtcat
     9361 tatcatattc aaccatgatt gccatactat ttgtactacc cgttggatct actggactac
     9421 taccaccaac tgagtattga attgttttta catgcttttt ctgttttaaa tatttttcaa
     9481 catctttcgc atgattcaac actgcttgct ccgtttcacc aggcttcggt gtataagtaa
     9541 tagctaaaaa tttatcgtca cctgctgaaa taaagctagt gcctagtctc ggtcctccaa
     9601 atacaatagt tacaaccaaa attaaagtac ttaatataat tacaatccac ttatgattta
     9661 gtgaccaatg caatactttt ttataagttg tactaacaac acctaatcct tcttgatgtt
     9721 gtttattacg acgtttaacg ccttttttaa atagtgttgc tgccaaagct ggaacgagtg
     9781 taattgacac taataacgat gctaataaac taaatgcaat agccaatgca aaaggtctaa
     9841 acatttcgcc tactgaacct gatacaaaca caagtggtaa gaagacgata atagtaacta
     9901 gtgtcgatga cattattggt ttaaatactt cagttgtcgc actgataatt aaattttcac
     9961 cttttaattg ttcttctgaa tctgttaagc gtcgataaat attttcaaca actacaatcg
    10021 aatcgtctat cacacgtcca atcgctactg ttaatgcacc taacgttagt atattcaatg
    10081 aaacatcact caatttcaga gcaataagcg ccataagaag tgataacgga atggatataa
    10141 tagaaattgc cgtcgtacga atgtttctta aaaacagcaa aataactata attgccacga
    10201 ttgtacctaa tgatgctttt tcaaccatcg tataaagtga tttctcaaca ggctttgcag
    10261 tatccattgt ttttgtgaca ttaaaatctt tattttcatc aacgaatgta tcaattttac
    10321 gttgtacatc tttggctact tgaactgtat tggcatcttg agctttagtt atttgtagat
    10381 taaccgcatc ctttccattc gttttagaaa tagaagtacg cacatcacca actgtaatat
    10441 cagctaaatc tcctagtttc gctgtcggca taccacttat attatttggt gctgacgctt
    10501 ttgaattttg ctgtggtgat gcctgattaa cgtctgacat ggctgaattt tgtttattgt
    10561 cactttggga ttgagattgc ccttgtcctc ctgcaaacgt taatggaata tttatgtttt
    10621 taaaagcatc aacagattga tattgaccat caacaacaat tgatttatct ttatcaccaa
    10681 attggaacaa tccaagtggt gttgttcttg ttgccgtttt tagatagttt tctacatcat
    10741 cagcagtcaa cccatatttt tcaagttcat tttgcttaaa tttaagggtg atttcacggt
    10801 tcgtctgccc atttaattgc gcattttgca caccatctac cgtttgtaat tttggtatta
    10861 attgttcatt cagtactttc gttacttttt tcaagtcatt ctctttattt gaaaatgaat
    10921 atgctaaaac cggaaaagcg tccatcgaat tacgtcttaa ttctggttga ccaacttcat
    10981 ctttaaattt aattttatcg atttcttttt taagctgttc ttctgcttta tccatatctg
    11041 tattattttc atattcaact gttacaattg aagcattctg tatggattgc gttttaacat
    11101 ttttcacata tgccaatgat cttacttgat tgtcaatttt actacttatt tcatcttggg
    11161 tactttgtgg cgttgcaccc ggcattgttg ttgtaactga aataactgga ttttgtacat
    11221 ttggtagtaa ttctaatttc aatttagcac tcgcatatac accgcccaag acaactaaaa
    11281 caaccattaa aaagatagca aacttattcc ctaaagaaaa ttgtaatagc ttttttatca
    11341 cagtacttcc ccctctttca ctatattcaa aaatatttta ctcaaccatt ctatattgtg
    11401 taaaaaaatc tgaacgcaaa tgacagtctt atgagcgttc agatttcagt cgttaatcta
    11461 ttttcgtttt aatttacgag atattttaat tttagctttt gttaaacgcg gtttaacttg
    11521 ctcaattaat tggtacaatg gctgattcaa tacataatca aattcaccaa tcttttcact
    11581 taagtatgtt ccccacactt ttttaaatgc ccataatcca taatgttctg agtctttatc
    11641 tggatcatta tctgtaccac cgaaatcgta agttgttgca ccatgttcac gtgcatactt
    11701 catcatcgta tactgcatat gatgatttgg taaaaaatct ctaaattcat tagaagacgc
    11761 accatataag taatatgatt ttgagccagc aaacattaat agtgcaccag aaagataaat
    11821 accttcagga tgttcctttt ctaaagcttc taggtctcgt tttaaatctt catttttagc
    11881 aattttattt tgcgcatcat taatcatatt ttgcgctttt ttagcttgct tttcagatgt
    11941 ttccatcttc tgctgccatt tagcaatttc ggcatgaagt tcattcaatt cttgatttac
    12001 tttcgctata ttttcttttg gatccaactt tactaaaaat agttcagcat ctccatcttc
    12061 atgcaacgca tcataaatat tttcaaagta actaatatca cgcgttaaga agccatcgcg
    12121 ttccccagtg attttcatta actcagcaaa tgtttttaaa ccttctctat cagatcgttc
    12181 cactgtcgta cctcgcttta aagccaagcg cacttttgaa cgatttcggc gttcaaaact
    12241 atttaataac tcatcatcat ttttatcaat tggtgtaatc atagtcatac gtggttggat
    12301 gtagtctttt gataaacctt ctttaaatcc tttatgttta aaaccaagcg ctttcaaatt
    12361 ttgcaaagca tctgtacctt tatcaacttc aacatcagga tcgattttaa ttgcatacgc
    12421 tttctcagct ttagcaattt cttttgcact gtctaacaat gcatttaacg cttctttatt
    12481 actataatca acaacaaaac cacgtgaaat ataacatagc gtatacggta atttaggtac
    12541 ttttttaaaa agtaactgcg caacaccctg aacttcaccg tcacgaccta cagcgattct
    12601 tcgcgcgtac catccagtta atttctttgt ttctgcccat ttcgttaatt gtaataaatc
    12661 tccattgggg tgggatttaa caaatgcgtc atgttcctga ttagtgatat gcatcttttc
    12721 catgatttat gatatctcct tctatttaac aataccttta attatacagt ttgtatctta
    12781 tagtgtcgat tcagagcttg tgtaagattt gaactcttat ttttgaaatg tccatgctcc
    12841 aattaatagt ttagcaagtt caaatttacc cattttaatt gtgaatcatt ttatatctat
    12901 gtttcgtgtt aaatttaatg ttatcgtaca attaatactt ttcaactagt tacctatact
    12961 tcaatatact ttcatcatct aacacgatat tcatttctaa aatgaaccaa cttgacttca
    13021 atgaataaat ttttcctcaa gcaaccacat taatgttcat atacaattac ccctgttata
    13081 atgtcaataa tctaacaatg aggtgtttga tatgagaaca attattttaa gtctatttat
    13141 aattatgaac atcgttgcaa tcattatgac attgagtcaa cctctcaccg tgaattactt
    13201 tagtttacgg gttatactta tctttttcac atttatatta tcaatctttt tcattttaat
    13261 taagtcatca cgattaaata atatattaac gattctttcc attgtgcttg ccattattca
    13321 tatgggcatt ctcgctcata gcacttacgt atatttatac taatggttca aagcgataaa
    13381 tagcacctct gataaaaatt gaatatggtg aagttgcttg tgcgtctttt atgataaccg
    13441 aatgatattt tgaaacttta ccatcttcaa ttctaaaata aatatcatca ttttttaaaa
    13501 tcaaatctgt gtaatggtca ttctcttcta caatgtccat atcaaaccat ttcaaccaat
    13561 tcgatactgt ttgtgatcgg tttttacttt tcacaataac agtttcaatt gaaaattgtt
    13621 tttgaaaata tttttgcaat tttttagtac gcatggaatc actttcttcc cattgaataa
    13681 aaaatggtgg cttaatttca tcatcatcct gattcattat ataaagcaat tgccacttta
    13741 ccttaccatc tttatgtgta tctctttcca tttgaatcgg acctactact tcaacccgct
    13801 cactttgtag tttattttta actgcctcaa tattatttgt acgcaaacaa atatttttaa
    13861 agccttgctc atacttctct tgaacaattt gagtagcaaa agcgactcca ccttctatcg
    13921 tttttgccat ctttttcaac ttttcattat tttctacatc cagtagctca atataatttt
    13981 cattgatata acctaattta ttgaatgttc catatttatg atgataccca cctgaatgta
    14041 attttataac atctcctgga aaactaaacc gatctaactg atctatataa tgaatgatgt
    14101 gatcaaattt caatatcatt agtatccccc tatttacatg taattacgct tattttaaca
    14161 aagtataaat atttttgctc ttaataatta tatattgatt tctaattgct cccgttttat
    14221 aattactatt gttgtaaaaa ggttagctaa gctaactatt ttgtcttagg agatgtcact
    14281 atgctatcac aagaattttt caatagtttt ataacaatat atcgccccta tttaaaatta
    14341 gccgagccga ttttagaaaa acacaatata tattatggcc aatggttaat cttacgcgat
    14401 atcgctaaac atcagcccac tactcttatt gaaatttcac atagacgtgc aattgaaaag
    14461 cctactgcaa gaaaaacttt aaaagctcta atagaaaatg accttataac agttgaaaac
    14521 agtttagagg ataaacgaca aaagttttta actttaacac ctaaagggca tgaattatat
    14581 gagattgttt gtcttgatgt acaaaagctc caacaagcag tagttgccaa aacaaacatt
    14641 tcgcaagatc aaatgcaaga aaccataaat gtgatgaatc aaattcataa aatattatta
    14701 aaggagacac acaatgacta atcaaggctc attaaataaa tcacctattt tcaccaaaag
    14761 ttttacaatc aacttttttg tcaactttat cgtataccta tgcatgtatt tgttacttgt
    14821 agttattgct ggctatagta agcaagcttt cgacgcttcc gattcattag ccggattagt
    14881 tgtaggacta tttattgtcg ggtctttaat tggtcgattt gctacaggta aatttgtaaa
    14941 tcaaatcgga cctaaacgat tattatttat tggtttaatt gctttaatta tcacgcaatt
    15001 actttatttt attgatggat ctttagcttt tttaatcttt gtacgtctca taaacggtat
    15061 cgcaactgca gttgtgacaa ctgcaacagg tactattgcg gcatacgtta cccctgttaa
    15121 tagaaaaagt gaaggcatca gtttattttc cttaagttta gtattaggta cagcgattgg
    15181 cccatttcta ggcatgttac ttattacaaa atacccaatt gatttactgt ttattatttg
    15241 tgtcatatta ggcatactcg gactcattat ctcattattt ataaaagttg atttcgaagt
    15301 aacaaataat aaaactgaaa ctaatgtcat tgataaacca agatttagta ttcatcaatt
    15361 tattgcaaaa gaggccattc ctgttgctgt aattatgctt ttaattggcg ttacctatgc
    15421 gtccatccta acttatttac aagcatttgc tttagagcga cacctagtaa ctgcagcaag
    15481 ctatttcttt atttgctatg ctatagcatc gttaattact agacctattg ctggtagatt
    15541 gatggatgat aaaaatgaaa atattattgt ctacccagca tttatcatgc ttttcctttc
    15601 atttgtatgc ttaatttcaa gctatcaaag ctggttaata ttacttgctg gtgcttgcct
    15661 aggtttaggg tatggaaatt tatcatctgc aatgcaatct attgcgataa aagtctcacc
    15721 tccgattaaa tatggtatcg cgacatctac tttttacgtt ggtctagatg caggtgtcgg
    15781 cttcggtcca tcattccttg ggttatttac tcatatgttt tcatatagtg aaatcttcgg
    15841 ctttatggcc gctttagcaa tcattactat gcttgtttac ttcttaattc atggacgtca
    15901 tgtaactaga aatgccatga actaaatatt tatattcaaa attaaaaagc atgccaatct
    15961 ctattcatca tttgtctgtc cccaagacgc aattatgatg tactgattgg catgcttttt
    16021 aagtttttag tttgtaacgc ttaacacttc tatttcttta gtaattttat ctaacaattt
    16081 cttttgagaa gtactataga aatagaatac ttgtctttca tccgtttcag aacgcaattt
    16141 agaaataagc tcttttttat agattttacg gataatcaca tcgattttac ttgtatccca
    16201 tagcatttca aaatgtaatg tatctctcaa ttctttaccg ctaatctttt cagcttcata
    16261 cactttaaaa agtactacaa attcttcaat tgaaatctta tgtgttgttt ttaaccagtg
    16321 ctttaactga cttaactccc tttctgcttc tataaaacgt tgaactttat tactcataat
    16381 atgatgcctc ctatgtttct tcttagatgt ttgtaattca tattattata caattactta
    16441 aatttattat atatcaaact tagcatttta tctattacaa atattaaatt gctggctata
    16501 tcttgtttag atttatatct tttggacaac ttgttgttta ttcgacaaca tttataccta
    16561 aaaaagatat attacaactc aatggcatgg agtgtgacat caatgttaaa gaaccactaa
    16621 agatttatta tgtagtgatt cttaagagtg ggatagaaat gatattctca taaaatttat
    16681 ttcgttgttc cccaacttgc acattattgt aagctgactt ttcgtcacct tctatgttgg
    16741 ggccccgcca acttgcacat tattgtaagc tgactttctg tcagcttctg tgttggggcc
    16801 ccgccaactt gtaaaaactt taaaaacgat catttctatt aaatctggca tagatatgac
    16861 cgtttttact ttaataaata atattgtatt ttagggagta agacagaaat attaaaacat
    16921 ctctaatgat ttaatatgta gtggtcccta cacattaacc accaatataa ttcatgttta
    16981 tctttttacg ttgacgattg gcaactgttt gagcagttct ctcatctgaa tatcgatcat
    17041 ctcttatttg ccataaagct ttaaattgtt cttttaattc ttcgtcggtc acgccagaac
    17101 gaataaacgc tttaacgtta aatccatcga cagttgcaaa taaacatccg taaaacttcc
    17161 catctgatga cagccttgcg cgtgtacatg tagaacaaaa tgattgtgaa acacttgtaa
    17221 tcaaaccaaa ttgaacacca ttatccttat ggcgataata ttttgctact tccccaaaat
    17281 attttggttc tacaggatcg atttcaaagt gctgctctat cattgtaagc atttcatctt
    17341 tagttacaac tttactgaaa tcccatccat tatcattacc aacatccata aattctataa
    17401 atcgaatctc tatatgttta tctttaaaat attcaagcat tggtatgatt tgatcatcgt
    17461 taataccttt ttgtataaca acatttactt ttacattcaa accaatagac gtcgcgtaat
    17521 caatttgttc taaaatcgta gtcgctttaa tattacgatt attgattgat tgaaatagcg
    17581 tatcatcaat agcatccaaa ctgacattaa ttctgcgcag cccagcatca tataactttt
    17641 gtccatgctt ttttaataac aaaccatttg tagtcaaacc aatatcttca ataccatcga
    17701 tttgatttaa tttagctata agtacatcta aatcccgtcg catcaatggt tctccacctg
    17761 taatgcgtat tttttttaca cctaattctg catatacctt agcgattcta gccatttcat
    17821 caaacgttaa aagttcattt ttaggtaaaa atacgaaatc atctccaaat acctctttag
    17881 gcatgcaata atcacaccta aagttacacc gatctgtcac agataaccgt aagtcacgga
    17941 tgggacgtcc tagtttatct tttatttgtt ctaccatttg tggacctcct aacagcttac
    18001 aatttttgaa ttaaagcgtc caaatcatgc tgataattta tatttttgta ccaatatgag
    18061 ggcgcatcta catcccttac atccaaataa tccgttgata attcatgata tacattttta
    18121 aaactgtaat tatcagaatg tagtgcttta gttatagcgc ctaatgcatt cggactataa
    18181 aatgcaattg ttggaataaa acgtccatct tctttaaaag ctgcgacatc taaatgattt
    18241 tcaataagat gagaaactaa aaactgatac aacgtgctta cagctttacc agtaatcatt
    18301 ggtgtatcaa cagaaacgac aaaaaacaat tcttcttcag gatgttgctt catgattgta
    18361 taaattcctg ctaatggacc tttatcatta tgattctcat catctataac aacatttgga
    18421 tatttaaatt gcgttgccaa ttgcgcattt gtactaataa taatttcatt gaacatattt
    18481 gttgattcta atgtcttaat tactctacta taaaaggtct caccgttcac ttccgcaaaa
    18541 gctttgggct taccaaatcg cactgaatga ccacctgcaa gaattattgc tttcatgctc
    18601 ccttaacctc cactaaccgg tggaattaat gcaacagtat cattaggttg aatgaaatcc
    18661 gatttttgta caaattcctc atttacagca acttgaaact ttttattatt gatttgcgga
    18721 taacgttcaa acaataaatc ttcaaattgt tgtacagtca atgcttgttc aagcacaata
    18781 tcttcctgtg ctttttgtaa tatatcttta atttctgcga agtaaagtac cttcatctct
    18841 cttattcctc cctcttcgct tcttcataat tccctttttg atgcccttgc cattttgaac
    18901 catcttccca aatttctttt ttccaaatcg gaacaatttc ttttatacgc tcaattgcat
    18961 attcatttgc tcgataggca tctttacgat gcggtgaaga aaccgcaatt aatacagcga
    19021 tatctgaaat ttgtaatggc cctattctat gaacaatact cgttatcgtt ccaggccatt
    19081 tttcatttat ttcatctcca atttgtgcca atttcttttc agccattgga atatacgctt
    19141 catattctaa atattccgtt ttgacgcctt tagtccattc gcgaacatga ccggtaaaaa
    19201 caactactgc accttgatat tcatttatag tgaattcacg atattgttct gtttgtatcg
    19261 gttctgtcac gatttcaaat tgtttcattc atttcaatcc tctatgttaa ttgtgtatca
    19321 caatcatttt taattttatt taataaccat tgctcaaatg ctgtaaaatc ttcatgctcc
    19381 cttacattaa tgctataaca aacattcgac aattgttgta atacttgcaa ctcttcttca
    19441 tttcgataga ctacgacttt ttcaaaatca gcatttttaa agccttcaac taatacgata
    19501 ttggtgtcaa ttgtaacaga tttttcaata atttgagtaa gattttgatt atctacacgt
    19561 gttacagttt gctgatattg aaaaccttgt acaatacttt gatctgcccc cgcttcaaaa
    19621 tgcttcatgt gatcgacgtc tgaatcctgt aattgaatat cttccttacc atgcccatga
    19681 tgtttaatag tagcaactgt ataaccatgt gactttaaga aagagacaat atgcctcatc
    19741 aatgttgtct taccagactt tttgtaacct acaatttgta aaatcataaa agaagttcct
    19801 cttcagcagc gtcagattca gtcaatataa tatctactgt atgccccgct ttaaaaccac
    19861 gtgaccctcc tggtaacatg accatacagt tagcatgtgc aatcgctaca accgcacctg
    19921 atttattgaa tcctgaaggt actacagtag ctccagcact tgttaacgtt gctttagcac
    19981 gtataaatcg tgtgaatggg tttgccttgg taaaatcttc cattaatgtt gctttaatta
    20041 tttgcgggaa gacttctagt gcgccacaca tatgtttaac agctggtttc acaaatagtt
    20101 caaatcctgt aaaacaagct gatggatttc cagataatcc aaacaaatac tttccatcta
    20161 caaatgcaac cgttgttacg ctaccaggac gcattgctac tttattaaat aacacttccg
    20221 cctttacagc cttataaatc tcaggtaaat agtcaaaatc tccaacagaa actccgcccg
    20281 ttgtaataac gatatcatgt ttttccatag cttctttaac gacttggatg ccactatcta
    20341 aatcatcttt ttgtgttttg taaataccaa cttcaagacc taatttttct gctaaggcac
    20401 gaatcattgg gccattagag ttacgaattt ttccatcttc taatacatca ttaacatcta
    20461 ataattcgct tcctgttgca ataacagcga cactcggttg cttaataact ttaacctctg
    20521 catagccata tgttgcaagg accgcgatag cccctggatt aattacttgt ccttttttta
    20581 gaacaacatc gcctgtcgtt gtttcttcac cttttaaaga tatattttca ttttttgaaa
    20641 atggtttacg aattgtaaat gtatcttcta gttcaatcgt ttgttcaaac ataacaacag
    20701 catctgcgcc attaggtatt tgtgctccag tcataatacg caccgcttcg tgatccccaa
    20761 ctaatttatc agaaactgaa cctgcaccaa tatgatcaat cactttaaac tcaatgcgat
    20821 tctgaccact tgccccttgt gaatcaacac tgcgaattgc aaaaccatca taaggtgatt
    20881 tatcaaacct tggtatatca taagtagcta caatatcttc tgctaagata tgatttagac
    20941 ttttttcaag tgctaccgta attgccggca ttgaactctg ctgattaacg atacgttgaa
    21001 ttgcttcttt aactgggatt gggtttcttt tttctactac cattaaaaat catctccaaa
    21061 acttttattt tttcatgata tactagatgc actaggaggg gtaaaatgac tgagtttact
    21121 catattaatc aacaaggcca cgccaaaatg gtcgatgtat cagacaaaca aattacaaaa
    21181 agaactgctg tcgctcattc aagcattact gtaaataaaa ctatttttaa gcagatttct
    21241 aataacacca ataccaaagg caatgtattg aacactgctc aaatcgctgg tattatggct
    21301 gccaaaaata catcaacaat tattccgatg tgccatccat taccattgac tggcatagat
    21361 gttcacttca gctgggatga aacaaatgca cccctttata ctttaaacat tcaaacaact
    21421 gtctctacaa ctggcaaaac cggtgttgaa atggaagctt taaccgctgc atcagcaacg
    21481 gctttaacca tttacgacat gacgaaagca gttgataaag gtatgattat tggcgaaacc
    21541 taccttgaaa gcaagtctgg cggtaaatct ggagattttc aacgccaatc aaatcaataa
    21601 attattttgt aagctcatga atcagatgat ttaattctgg tttaatgagc ttttctaatg
    21661 ctaatttaac tgcgcctgtt gatcctggaa tcgaaaatat caatttatta ttaactgtac
    21721 ctgctacagc acgagacaat aatgcacgcg tgccaacatc ttcaacataa ctcaaatatc
    21781 taaacaattc cccaaagcct tctatctctt tagttaaaag tggttttact gcttcaatcg
    21841 tcacatcacg ttgtgcaata cctgttccac cagtcgtaat gatgacatca atatcttctt
    21901 ctaaccactt cttcacctgc gtcgtgatgg ctactttttc atctttcaca attgtataat
    21961 gtgcgtcact cacttcaacg tcatctgctt gtaatagttg gcgcacgcat tgaccacctt
    22021 tatctgtatc aaagtctcta gtatctgata ccgttagtac ggctgcttta actgtacgat
    22081 tcaatttaac gttttgatgt tcgcccataa tattacactc cttttgtctt atcttttttt
    22141 atccaaacaa taaattcatt agatgtgtgg catctgatgt gcgtgtcatg ccatgtatta
    22201 aaaaccttcc acctttaaaa gcaacaatgc ggtgtccttt aaattcaaac ataaccatat
    22261 acgaattact gcgataattt agttgatgtt gttttaaaaa ttgaacaaga atgtcgtgtg
    22321 taattgatgc attttcatac tgtacagtgt ctctaccaca caatgttgca taacgttgtt
    22381 cattcttgtt taaatacgga taacttggta catctccaca agttgtacag tctgaacgtt
    22441 gcattttact gaaaccaaat gaataatgac taccatccca aatatcgcca taagttattt
    22501 ttgtgtcaat tggttgttcc gttaatactt tcatcgcatc tcttaattgt aaacttgttg
    22561 ccatcgtcac ggcaggttga atgaccccta ccgtatcaca tgttaaattt aatgctggca
    22621 attgtggtac caaacagtta aagcaaggtg ttttaccagg tataaatgca gcttctgtat
    22681 atgtactctg tacaacacca ccataaatcc aaggtatacg atgtttatat gcaaaatcat
    22741 caatcagttg tcgtgtttca aagttatcgg ttgcatcaat aataacgtca acgtcctgtc
    22801 catgtgtttc caaaaaataa taatccacat gggcaatata atcatcaata tcaacatcac
    22861 tacgtaacgc tagcaaatgc tttttagctg caaccacctt aggcatcatt ttcaaagcat
    22921 cttcttcagt aaacaatgtt tgtctttgta aattactaaa ttcaatataa tctctatcaa
    22981 caatgattag tttagcaatg cctgctctaa caagtccttc agccacatgt gtacctaatg
    23041 cgcccatacc aataatcaac gcacattttt gatttatttt gctttgacct atttcaccta
    23101 tttgtttaaa taaaatttgc cttgaataac gttcttgact cacattaagt tatcctttct
    23161 ttttatcatt actttcatta tatgacgcgc gtcaatgtct ctcaaaaaaa ctgcccctaa
    23221 aaaagagttt aaaacatcat taaaatgctg atagaagcat tcacttctac aattttaaat
    23281 acgttctaaa ctctttaatg gcaaataaat gattacccaa ttgtaataat ttcatgagcc
    23341 atttgttctg cttcatagtt tgaatgtgtc acaaatatga ttggtatttg ccattcgttg
    23401 aaaatacgtt taactaatgt aatactctca tcttttgtag tatcatccaa actagaaaaa
    23461 ggttcatcta ataaaattaa atctggtttc gtgctaagtg cacgtgcaag tgctacacgt
    23521 tgtgcctctc cacctgacaa tgtcatagga tattgtttca tcaaatgatc aatgtttaaa
    23581 gtttgaatta attgatcgat gtgttcagat ggttcagcca taaaagtaat atttttatag
    23641 accgtcatat taggaaacaa ttggtagtct tgaaacagat atccaatacg tcgttgttga
    23701 attttaacat tcacgttttt tgccgtatca gtaagtaatt gcccattcac ttcgataata
    23761 gcttcatctg ctttacgtaa tccggcaatc atatttaaaa cagtagtttt accaatgcca
    23821 gatggaccac gaactgcata aatttttggt tcagtatcat ctatattgat gcgaattaaa
    23881 gtgttcttta attgatattt cacattgatt ttaagcatct agtcgacctc cttatattta
    23941 tctttattca ataaattaat tgtagatata accacaatag agaatgcgac tagcactaat
    24001 acccataacc acgcttcatt ttctctacct tgttccacta agaagtatat ttctaaaggt
    24061 agtgtattcg ttttatttgg aatatatcct gcaaccatta atgtagcacc aaactcacct
    24121 aatgcacgag caaaactcat cattatacct gctaaaatag agcgtttagc taatggtaaa
    24181 attaatttga ggaaaatttt cgtttcactt gctcccatcg ttctagctgt attaatcatt
    24241 ttcgtgtcta tacctctgaa gccttgcaca gtatgttgat acattagtgg aaaactaaca
    24301 atgacagatg ctatcacagc acctgtcaaa gtgaacacta caggtaaatg tagtacattc
    24361 gcaaagaatt gaccgattgg tcctcttggc gagaagatga ttaatagaat aaaacctaat
    24421 accgttggcg gcaaaacaat aggtaatatc aataaacttt ccaatacttt aacccacgaa
    24481 cccttacgat gatacaacca tttagatata aaaataccta aaaccgttac aataatcgta
    24541 ctgattacag caactcgtat tgatatccaa aaaggtgtta agtcaggcat ggattacaac
    24601 tccttatgct gcaaagtggt attcttttaa tatttcttta gctttatctg attttaagaa
    24661 ttccatccac tcttttgcta atttactatc tgatgtagca ccagcttcgt atgtgattgg
    24721 tttcttaagt tctacttctt taattacttt tacagtatca atttttttgt tttgtttata
    24781 taagtcagtt ttatacacaa aaccttgttt cgcattaccc ttttcaacat aatttaatac
    24841 ttgttttaca tctttagcat aaacgatttt actttcgact tctttaaata agttattgtt
    24901 atctaaatac tgtttcgcat attttcctgc tggtacagtt ttcacttcac ctaatgctaa
    24961 tttatcattg tcttttaagt cttttactga agtgtaattt gaatctttat caccaattaa
    25021 tactagacta tttttcgcat atttatatgt atcatgcgct ttattcttgt cttttaatgc
    25081 atctacatct ttagtatttg cagacataaa tacgtcaaca ggtgcgcctg attcaatttg
    25141 ttttcttaat gcccctgatc caccatagtt aaatttaata tcagcatttt tatgctcttt
    25201 tttaaattct gaagctaatt tcttggttac atctgttaaa cttgctgccg ctgcaacttg
    25261 aatctcttgt ttcttaccat tgtctgcgtc atctttttta ctttcattat tatcgttaga
    25321 attagagcaa ccagctaata ctaaaaataa tgccattaca atagctataa aacgtttcat
    25381 tttcattgag tgaaccccct tttttaataa tatttacatt ttcattatac acttactatt
    25441 atatttgaaa agaaccgttg cctgtttata tattgattca aacaattgtc aaatttgcat
    25501 aagattgata tttattaatt gatcccctag tatgtatact ctcgaattca atttaaatca
    25561 aacagattgc tcgctattat tttttataaa tcaaatttca aacttaagca aatatgttaa
    25621 aattttaagc aaattgcttt aaaataaagt ttaagctaat taaaggggtt agataatgaa
    25681 caaagatgta tctttaggtc aaccaattgt tagatatgaa gatggaaaat tattcaatac
    25741 aacagatcaa tatgtgacag aatttccact tacaattatg gtgaatggtg aagaatttgc
    25801 gactgttata tgcagtccaa caaaccttga ggagttagtc attggatttc tggcttcgga
    25861 aggtgccatt ttaaagcgag atgaattaaa atctgtgctg attgatgata gtaaaggttt
    25921 tgcccatgtt gaacttaata aagatttagg cgatcgcttt caatattcca caaaacgaat
    25981 gattgcatct tgttgtggaa agagtcgtga gttttatttt caaaatgatg ctgctatcgc
    26041 taaaacatct atgtctaaaa tcactttaac tccaatgcaa attattaaca tgatgactcg
    26101 attacaaagt gcaagtcata tttaccaaga aacaggtggc ttacataatg ctgctataag
    26161 cgatggtttg acattttttg tacatcgaca agacattggt agacataacg ctctagataa
    26221 attatatgga ttttgtattc agcgacatat tactgtccga gataaagtat taatttttag
    26281 tggacgtatt tcatcagaaa ttttaattaa agccgcaaaa attggtgttg gcgtcatttt
    26341 atctaaatca gctccgacaa cgcttgctgt aaccttagct aatgacctaa atattacggc
    26401 agtaggattt attcgaaatg gtggctttaa tatatatagt catccagaac gtatcatcga
    26461 ctctgaacaa taacatattc attatagtgt gtcataatga acgatattct acatgaatca
    26521 acaatataaa agcactattt ttataagtat aagtagctca aaacaattta aaaagcagta
    26581 agttgatttc tactcgaaac cagcttactg cttttttatt ctaatacaaa cctatcatca
    26641 atcgttaatt tccaatatct tcttttaata tttgataaca aaatgattgg taaacataac
    26701 cttgctttgc atacatatct tttgctgtat cttcaccatc tgcaactaat atgattggtt
    26761 tgtgattttt tgatatggcg tattcagcta tcaacgattg aattgtagat ccaattcctt
    26821 ggtgccgaaa ttgttctaat acaccaaatc catctaattc aatgtaattt tcactttcaa
    26881 tgacatctac aacgccaata ggttcattat ttaaataagc tactaagcgt ttaatcacat
    26941 ctttttgata atgttccctt accatttctt catgtgcatc tgcatagtct tttccaaatg
    27001 gcaaactaaa ctgatatgca acttttaaat aggcatctat atgcgactcg tcaacgatgg
    27061 caatttcaac gtcagagtta cgcggcaaat tggcaagcgc atcactttct atggcataca
    27121 attccataat acctagttca aaattcatat ctttaaacat agccatccaa tgattatcaa
    27181 gttgttcatt ttccgggaaa ctaaacgcat aatgatttga accttgattt gtatgtaatt
    27241 gatgttgacg ttctaagtct ttcagccatc gctcttgggt aggcgtcttt ttatatatcc
    27301 atgtgttgct tgtgtatcgt tgtggaaaag aaggcgttaa ataaattaac tgctcgtcgt
    27361 cattttcaaa tatagtgccg ttacaatata tatctgacat acttatacta gccattgaca
    27421 cctactcctt tcacttttat aaatttcaaa gcaacttatt caattacttt attatcttaa
    27481 cttaaatatg attattttaa tcccataatt tgacgaaact gcgagtgatt aagtaaagct
    27541 gtaccaatca aacttgctac aattgctttt aatatatcac caggcaaata agctaatgaa
    27601 attgaaatag ctttcgtaaa tggtatgtta ataatcatgc ccataatcaa tgtaccaata
    27661 acatctaatg ctataacacc aaaaactaaa ataccaacga ataaaatcca gaaattaatt
    27721 tcattgatga atctatcacg aatcgcacca atcatgaatg ctacaactgg atataatagt
    27781 aaaaaccctg ctgaaggacc tgcgaataca ccaatgccac cgcgaccgcc tgataacagt
    27841 ggcaagccag caactactaa taataaaaag acgataacac ttaatgtgcc atatttacgt
    27901 cctaaaatca cacctgctaa gaaaatacca atgttttgaa gtacaattgg tactgaagaa
    27961 aatggtagtg gaattaccgg taccaatcct aaaatagcga taatcgctgt cattaaagct
    28021 gtatatacta actgttttgt tgtcattttt tacttccttt cgtatttcat gaccattatt
    28081 atagcaccat tgtaaacttt aagaaacgaa aagtttacaa taagttgttc tatataaatt
    28141 aaaaccttaa ttattctaca cagctgttaa tttacttttt tagcaagtgc agttatataa
    28201 tcaaaaaatg cgttgttatc tacatgattt atgacattaa ttttgcgccc atctttacac
    28261 tcaaatgtct taccttgact tggtccataa cttattacat cgactttttt ctcaattgaa
    28321 tgaaccaagt ccttgttacc aatataagca gtcgttaaaa catcccataa aaagtaagta
    28381 gaatttgtta taaagtgtgt taatggtggt accgctgcat aacttactcc taaaaaatca
    28441 atgcccgtat attggcgttc attcgcccat ctttgtctaa catctaacgt tagcggtact
    28501 tgattcgtac tttctaaagc aaccatatca atctctatat cgctatcaaa aacaatttta
    28561 accgcttctg gatcccaata tgcattccat tctgcagaac catcatgctc aggttcttca
    28621 acatttcctt ttggtaaaaa ggtaccaccc atccaaacta atttttctat atactgaacg
    28681 atagatgaat ctttttgtag tgcttttgct aaatctgtaa gcgggcctgt aaataataat
    28741 gttacttttt ctgattgtct ctttaaagtt tgaataatgt cttcaaaggc ttctttgtcg
    28801 ctcacatttg aagcaacatg tttgactggc tcatttaaaa ttggcaatgc gtccataaaa
    28861 aaggcatgca tacgccattc tttaggaaat ggattttttc ctcgttcata tgatggcgca
    28921 acttgaatat cttcatttga aaaacgatta ataattttta ctgatgcgct caaagatggc
    28981 tctaaataac aatcagcacc aattgtactg acccctatca attgaacgtt ttccatttgt
    29041 aataataaaa atagagacac taaatcatct acaccaccat catggttaaa ataaactttc
    29101 ttagtcactc gaaatccccc ttacatttca ttttccatta acaatattat gttttataaa
    29161 acgttgttat atatctttct acaaaatcat aacattctgt tgtaatcctt ttaaacctct
    29221 tcttttattg atagccattc cctgatatcc cttccataaa gtatgaacta atataattat
    29281 gatataactt attaataaag agatacattg agctattttc tcaggtatac tactttagct
    29341 ataataatac taatctatca ttaattaaat tgaacgataa caacggcact tattattaaa
    29401 aaaaatacaa atatctaact ttctagctat aaaaataaga gcctaataca atatcaaaag
    29461 attgatatgt ttcagactct catttcacta acttatatag ctgaattatt ttgcagcttt
    29521 aattaatttt tcttttaaat ctttacgcat gaaatctaat gtataaggat cgttgtacca
    29581 gtatgtacca gcatcaactt taacaatatg tccttcttta gtagctttca aattcttcca
    29641 catgtttgtt gattcatatc ctggtgtagg tttaccttca cttgtactca caatgtaatc
    29701 accagcatat ttttcaattt cttcttgttt cacttcagcc caacctgctt ttgcagttaa
    29761 cttttgttgt tctggttgca tttttaaacc aaatgcttga tataatactt ctccaccacg
    29821 accccagtta tcgccgtaag tgtataattt tttatcaaat tcatcaaaca atgacactgt
    29881 tgcatcttgt ccaattgctt ttttaatttc tttatcgtct ttagcagttg tttcttccca
    29941 atctttcttc caagctttta ctttatcttc tttaccaaca attttcccta acatttcttg
    30001 ttgttctaaa tatttatgct tattatagtc aacaactact gttggtgcta ctttttgata
    30061 ttttttaata tctttgtcag tagagtatac aataattaaa tctggctttt ctttagcaac
    30121 tttttctaca tcgccatcac caatttttgt aacaccttta aatttatctt ttaatacttt
    30181 gctttgatcg acttgttgat ttacagctac aatgtttgca cctaattttt taagtccacc
    30241 agcatatgtt ggcgcaacta ctgcaatgcg tttagggtct ttcggaatat ctaccgtttt
    30301 gccatcgtcc attttataag atttagtttc agctttgtta tttttttcac cttggttccc
    30361 acacgcagct aacactaata acataattat taatggtaat aatagttttt tcataatttc
    30421 ctcctattga aaatgattat caattattcc aaaattatac aagattgcat atacatttac
    30481 aatagctagt gctatttatt tttttatttt cgcaaaaaat ttaacaaaat atcttaacat
    30541 ccctataaat ccccacaaaa aagggtaaat ccgaacgtca aatttcatct ctttatgcca
    30601 atcaacgatc gatttacctt tttaaattta aaatgtatct gtaatactct tagcaatatt
    30661 tgtatatgct gcatcttcca taggtggatt gtgtaatcct gcacgcatgt cacgatagta
    30721 acgttgtaat ggtcgattca tttctaagct tttagcacca actattctca tcgctaaatc
    30781 aatcacttca agcccttgat tcattaccat cactttactc gcacttgttg aatttcttat
    30841 ctgactgtca tctttatatt gttgatagcc ttttgctgta ctccataaaa attgtctcgc
    30901 acataacaat agcgtttcca ttttccctaa attttgttgc acagttggca atgttgcaat
    30961 cgttccttca atactattag ggctatgttg gattgcaaag tctacagcat aatttctagc
    31021 agcttgtgct atacctaaat aacagcttgg tatatgcaat atccaaccat ttggcgcttt
    31081 acttttctct gtttccacta aatgttttaa tggtactttt acatcattta atattaaatc
    31141 atgactttct gtcgctcgca tacctaatac gtcccaatta tcggcaattt caacaccagg
    31201 caaattcctg tccactaaga aaaaaccaac accttctaat tcttctatat aagcagcaac
    31261 aataatatgt gttagtgctt tactcattga tgtatatgtc ttcacaccat ttaaaatata
    31321 cccatcatca gctttaacag catgtgtact tggtcttccc cctcttgttg gactacccat
    31381 ttcagcttca ctaactgctc tattaactaa tgcaccatta ttaatttcaa cagcaaattg
    31441 ctccaacata tcttgagacc ataatttctg ttcataaatt tgtcctacga cactcacatg
    31501 ccaaccaata gataatgctg tggcaccatc aagttcgcct aaaaatgatt gcaaaataac
    31561 catgtcttct atggtcgcac cttcaccacc atatgctttt ggtaacgtta attttccata
    31621 accttcttta attaaccatt caatattttt atatggaaat tggctatgtt gatcattata
    31681 ttcagcttta gccttaaatg tatctctaat tactttgaat ttttcaatcc attttctttg
    31741 aatttccgtt gttattaaaa aggaattatc catatcattc atattagtca ccccacacaa
    31801 cacacaatat aaattattta ttcctagttt aacactcgac tttaaaaata aaaaggaacg
    31861 tgctgtgaac aattaaaaga aaactgataa ttcgattaag tatgcactta attttatcca
    31921 taaaaataca ccttaaacta tacaattcgc tgtatagttt aaggtgatat tcaaacaata
    31981 ttaggtttga tttaactatc aagcatctac tttgtcatgt ttaataccag caaataataa
    32041 aatccatgtc acaataataa atggcaatgt taatgcaggt aagccaaacg gttcaagcaa
    32101 tgttgttgta cctagttgca ccactactgt taataacaca cctaaaaaag tcgaaatata
    32161 agggttaatc gctgttttaa atgtatatcc tagtgcgatt gccattaata caaagttata
    32221 accgaataat ccctgattaa tatcatcata attaccacct aatagagcta caatgataaa
    32281 gcttaacaaa ctagctataa cagctaagag tgctgctttt cttgatgcta tcaatatgcc
    32341 gattaaaata catacgccac caattacact cgcttcgata aatacttgac tgaatccttc
    32401 aaataatgac tgaatgaaat gtattctatc attgttgcta aaattaaccg tttcaatgtt
    32461 ttgaggcatt aactttaacg atgtatctac aaatttaacc tgtcctgaaa gtaaaattgt
    32521 aaaccaagtc acaatgacaa aaggcatcgt cagcatcgga actttatatg gtcttaaaac
    32581 ttctctcact gcagcagcaa ctggtaacgt taataaagtt gctatcattg ttataacaat
    32641 atctaatcct gacttatcta aaaagattgt taacgcaatg gcagttaaca ctggattaaa
    32701 tccggctaac ccatcattaa tctctgcttc actataatta atgtaacgtg caaatgcata
    32761 agctatgata ctacctacaa tagccgctaa tccaattgtc caatcggcta caaataatcc
    32821 tattaagata aataatcctg tccatttatt actaattaac acaacctgag aaatattttt
    32881 caaaagtaca tctattatct tcaaatcttt cacctttcct ttgaatcatt actcattaat
    32941 atactactat tctatgaaaa gttatattaa aaactatttt ggtttttaca taaatatatt
    33001 ccactatttg ttttcgatta gattgtttgc ttcaatttcg gatatgctaa aaatataatt
    33061 ttctaattac acattgaact ttgtatatta cttgtttatc acaacaatta ggattaatat
    33121 aaattttgta aattgtatct aatatgaaat tgggggtttt aatttgcatt ttacacaacg
    33181 agagcaagac aaattaatga ttgtagtggc ggcggaagtt gcacgtcgtc gtaaagcacg
    33241 tggtttgaaa ctaaatcatc ctgaggcatt agctttaatc agcgatgaat tattagaagg
    33301 tgcacgcgat ggtaagaccg ttgcagagtt aatgagttat ggtagacaaa ttctaaacaa
    33361 agaagatgtc atggatggtg tcgaacacat gattacagat atcgaaatcg aggctacgtt
    33421 ccccgatggt actaagttaa tcacagtaca tcaccctatt gtttaaagga ggcagtcaaa
    33481 tgatacctgg agaaattatt acaaaaagta cagaggttga aattaataac catcatcctg
    33541 aaacagttat cgaagttgaa aatacaggag atcgaccaat tcaagtgggc tcacattttc
    33601 atttttatga agcaaatgca gcattagatt tcgaacgtga aatggcatat ggaaaacatt
    33661 tagatatccc agctggagca gctgtccgat ttgaacctgg ggataaaaaa gaagttcaat
    33721 tagttgaata tgctggcaaa cgtaaaattt ttggttttcg tggtatggtc aatggtccta
    33781 tcgatgagtc acgtgtctat cgcccaactg atgaaaatga tgaatatgca ggtgtattcg
    33841 gagataacgg tgctgaaaac gtgaataaaa aaggaggaaa aagatcatga gctttaaaat
    33901 gacgcaaaat caatatacga gcttatacgg tccaactgtt ggagattcca ttcgtttagg
    33961 tgatacgaat ctatttgctc aaatagaaaa agactatgcg gtttatggtg aagaagctac
    34021 ttttggtggt ggtaaatcta ttagagacgg tatggcgcaa aatcctcgtg taacacgtga
    34081 tgacgtgaac gttgcagacc ttgtcatttc taatgccgtt attatcgatt acgataaagt
    34141 ggttaaagct gatataggca ttaaaaatgg ttatattttc gccataggta atgccggcaa
    34201 cccagatata atggataatg tcgacattat tataggttca acaacagata tcattgccgc
    34261 tgaaggtaaa atcgtcactg ctggtggtat tgatactcat gttcatttta ttaatcctga
    34321 acaagcagag gtcgcattag aaagtggcat tacgactcat attggtggtg gtactggtgc
    34381 ttcagaaggt tctaaagcaa caactgtaac tccaggtcca tggcatattc atagaatgtt
    34441 agaagctgcc gaaggtttac cgattaatgt cggttttaca ggtaaaggac aagcaacaaa
    34501 tccaactgca ctcattgaac aaatcaatgc cggagcaatt ggattaaaag tacatgaaga
    34561 ctggggtgca acaccatctg ctttgagtca tgcattagat gttgctgatg aatttgatgt
    34621 tcaaattgca ttacatgcag atactttaaa tgaagcagga tttatggaag acacaatggc
    34681 tgctgttaaa gaccgtgtac ttcatatgta ccatactgaa ggtgctggtg gcggtcatgc
    34741 gcctgattta attaaatcag ctgcattttc aaatatttta ccttcatcta caaatccaac
    34801 tttgccttat acacataata ctgtagatga acatttagat atggtaatga ttactcacca
    34861 tttaaatgcg gctattcctg aagatatcgc attcgcagat tcacgtattc gtaaagaaac
    34921 gattgcagca gaagatgttc tgcaagatat gggtgtattc agtatgatta gttccgattc
    34981 acaagcaatg ggccgtgtag gtgaagtaat tacacgaaca tggcaagtag cacatcgcat
    35041 gaaagaacaa cgtggtcctt tagatggtga ttttgaacat aatgataata atcgcatcaa
    35101 acgttatatc gctaaatata caattaaccc agcaattaca catggtattt ctgaatatgt
    35161 aggatctatc gagccgggca aactagctga cattgtctta tgggacccaa ttttctttgg
    35221 ggttaaacct gaattagttg taaagggcgg attaattaac tctgccgtaa atggcgatgc
    35281 aaatggttct atacctacat ctgaaccgat gaagtaccgt aaaatgtatg gtcaatacgg
    35341 cggaaacctt acaagtacgt caatgacatt cgtgtctaaa actgcttatg aaaatggtat
    35401 caatcgtgca ttaaatttaa aacgcatggt gcgtccagtt aaaaatatta gacaattatc
    35461 taaagcagat atgaaaaata acagcgcaac acctaaatta gacgttgatc cacaaacata
    35521 tgaagtatat gtagatggag aaaaaattac aagtaatgca gcaactgagt taccattaac
    35581 tcaaagatac ttcttattct aggaggaaca tagaatgatt gttgaagaaa tccaaggcaa
    35641 tattgccaat ttgtcaaatt cagaaaaaca aaagcacgtc gaaaaagtat accttgaaaa
    35701 ttcagatctt gttaaacgta ttcaaagagt cgttacagac catggcactg aaataggcat
    35761 tcgtttaaaa caacctattg acttacaata tggagatatt ttatacgcag atgaccataa
    35821 tatgattatt gtggatgtta attcagaaga tcttttagtg attcaaccaa gaacattgca
    35881 agaaatggga gatatagccc atcaattagg aaatcgtcat ttgccagcac aatttacaga
    35941 gactgaaatg ctagtacaat atgattattt agttgaagat ttattaaaaa gtttaggcat
    36001 cccatatgtt cgagaagacc gtaaagttaa taaggctttc agacatatag ggcattctca
    36061 tgattgatca tacacactta agattatttc agttctgtga ttcacagttt ccaacaggtg
    36121 ctttcagtca ttcatttggt cttgaaacat atattcaacg aaatataatt catgacgatc
    36181 atacttttat tgcttggtta aaaatgtttt tacaagagca acttacctat tctgatggtt
    36241 tagccatgcg tttagtttat gatgcattgg aaaatgatga tacacaaaaa gtattacaca
    36301 tagataaact gatgtttgtt caaaacttac ctaaagaaac acgtgttggt gcaaaacaaa
    36361 tggggactcg catggttaaa ttggctttag aactttataa tagcccatgg attgcttggt
    36421 atcatcaaca aatgcaagat aagaaagcaa agttaaatcc agctatttgt tttactatgc
    36481 taggccatca tttaggtgta gatattgaaa cgattattga ttactattta tatcaaaatg
    36541 tttcaagttt aactcaaaat gctgttcgtg ctattccact tggacaaact gctggtcaaa
    36601 agatagttac tcacatgatt ccttatatag aaggaacaag aaagcagatt ttcgaattaa
    36661 aagaagcaga ttttggcatg acagcgcctg gtttagaact aaatcaaatg gcgcatgaga
    36721 acgtcaatgt tagaattttc atatcatagg aggttataat tgtggcaaat ccgattaaaa
    36781 ttggtattgg tggtcctgta ggtgcaggta aaacacaatt aattgaaaaa gttgtaaaac
    36841 gtctttcaaa agaaatgagt atcggcgtta ttacaaatga tatatataca aaagaagacg
    36901 aaaagatatt agtaaattca ggagttctac ctgaaagtcg tatcattggt gttgaaactg
    36961 gtggatgtcc tcatactgcg attcgtgaag atgcatctat gaactttgca gcaatagacg
    37021 aattattaga acgtcatgac gatatagaac taattttcat agaatctggt ggcgataact
    37081 tagcagcaac atttagccca gaacttgttg acttttcaat atatattatc gatgttgctc
    37141 aaggtgaaaa gattccacgt aaaggtggtc aaggtatgat taagtcagat ttctttataa
    37201 ttaacaaaac tgatttagct ccctatgtag gtgcatcatt agaacaaatg gctgaagata
    37261 ctaaagtatt tcgtggtaaa cgtccattta cttttactaa cttaaaaact gacgaaggtt
    37321 tggatgaagt tatcgattgg attgaacgcg acactttact caaaggatta tcataatgga
    37381 tgaacaacaa tggactgggc aacttgattt aacagtgttt tttgatggca accgatcagt
    37441 atcaagagat attttctttg aaaaagcact taaagtgata cgtccagttt atctaaatca
    37501 atctaccatt cctacatttt atatagtaaa tgtaggtggt ggttatttag atggagatcg
    37561 ttaccgtatg aatgtgaatg tcgaagataa cgctaaagtg acattgacat ctcaaggtgc
    37621 aacaaaaata tacaagacac cttccaatca tgttgagcag tatcaaactt ttaatttgaa
    37681 agataacgca tatttagaat atgtcgctga tccaatcatc gcatatgaaa atgctaaatt
    37741 ttatcaacac aatacgttca atctcaataa ttctagttca ttattttata ctgatatttt
    37801 aactcctggt tattcaaaaa ctggcgaagc cttcaagtat caatatatgc atttaataaa
    37861 tgaaatttat attgaagatg agttagtcac atatgataat ttattattga atcctaataa
    37921 acaatcgatc aatgaaatag gttatatgga acattactct cattacggct ctgcttactt
    37981 catacatgaa gatgttaacc aaaaactaat cgattcagtt tatgaaacca ttagttctta
    38041 tagcaataca ttcgattgtc gtgttgctat ttctcaattg cctacacatg gttttgcagt
    38101 tagaattttt gcttatcgca cacaaattat agagaaaata cttggaatca tccaaagcta
    38161 tatcgcagaa aatatttatg atcgtaaact tgattttcta agaaaatatt aaaataaaaa
    38221 gactagtgta ccttgtttca agcttaataa gctgaaataa ggtatggcta gtctttaact
    38281 ttaattaaag catcttcaat atttaaattt taagttaatt ttatgtccca atgctgaata
    38341 aataacttcg ttttaataaa ttgttatcgt tcaatgttat taaacgcaat taaccttgat
    38401 ttaattttta atgtattctt ctaattctga aatcagtttt tgaatatttg ctttttgtgt
    38461 atctgtaaca taaacaataa ctgttctttc gtcttgtaaa cttcttttct ttgataacaa
    38521 ttttaaatct tttagctttt gtaaagcttt agttaaatag taaggtttga actctgagca
    38581 cttagcaatc tctttagatg agatttcgtt agactcactt cttaaaatat gatttaaaat
    38641 ataaatttct tcatagttca aattgaactt cttttttgta tctctgaaaa acttcttaac
    38701 ttgaaatgtt gcgttgacta aatcattaat gtcattaatt ttactcatta ttaaaccact
    38761 cctctgatgc acatcttgtt gtagcaaggt tcacatgtaa tttattaaat cgaaactcta
    38821 tccaactatg ttataaagtt cattctaaac aaataagtat aaaaattcaa tatttttatt
    38881 actagaatat ggttaaatac atttatttct tataataatt tgatgttta
//
LOCUS       DAMZKC010000004        35613 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.004, whole genome shotgun sequence.
ACCESSION   DAMZKC010000004 DAMZKC010000000
VERSION     DAMZKC010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 35613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35613
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.004"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            75..1259
                     /gene="rocD"
                     /locus_tag="POB55_000139"
     CDS             75..1259
                     /gene="rocD"
                     /locus_tag="POB55_000139"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HDI6499137.1"
                     /translation="MNSIIELTDYYSSNNYAPLKLVISKGKGVKVWDTDGKQYIDCIS
                     GFSVANQGHCHPTIVKAMTEQASKLSIISRVLYSDNLGKWEEKICHLAKKDKVLPLNS
                     GTEAVEAAIKIARKWGSEVKGITDGQVEIIAMNNNFHGRTLGSLSLSNHDAYKAGFHP
                     LLQGTTTVDFGDIEQLTQAISPNTAAIILEPIQGEGGVNIPPKGYIQAVRQLCDKHQI
                     LLIADEIQVGLGRTGKWFAMEWEQVVPDIYILGKALGGGLYPVSAVLANNDVMRVLTP
                     GTHGSTFGGNPLAIAISTAALDVLKDEQLVERSERLGSFLLKALLQLKHPSIKEIRGR
                     GLFIGIELNTDAAPFVDQLIQRGILCKDTHRTIIRLSPPLVIDKEEIHQIVAAFQDVF
                     KN"
     gene            1298..2329
                     /locus_tag="POB55_000140"
     CDS             1298..2329
                     /locus_tag="POB55_000140"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HDI6499138.1"
                     /translation="MIKVGIVGGSGYGAIELIRLLQTHPHVTIAHIYSHSKVDEPLKL
                     TFPHLQHIMQHFEALTVDNNDCDVIFFATPAPVSKTCIPPLVEKGIHVIDLSGAFRIK
                     NREIYEAYYKETAAAQDDLNHAIYSISEWQSFDNNGTKLISNPGCFPTATLLALHPLI
                     SEKIVDLSSIIIDAKTGVSGAGRSLSQRVHFSEMNENLSAYAIGNHKHKPEIEQYLSI
                     IAGQDVSVIFTPHLVPMTRGILSTIYVKLSSEYTTESLHKLMTSYYANQPFVRIRDIG
                     TFPTTKEVLGSNYCDIGIYVDETTQTAILVSVIDNLVKGASGQAIQNLNILYDFEVTT
                     GLNQSPVYP"
     gene            2341..3582
                     /gene="argJ"
                     /locus_tag="POB55_000141"
     CDS             2341..3582
                     /gene="argJ"
                     /locus_tag="POB55_000141"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HDI6499139.1"
                     /translation="MKHQETTSQQYNFSIIKHGDISTPQGFTAGGMHIGLRANKKDFG
                     WIYSSSLASAAAVYTLNQFKAAPLIVTEDTLQKSKGKLQALVVNSANANSCTGQQGID
                     DARQTQTWVAQQLQIPSEHVAVASTGVIGEYLPMDKIKTGTEHIKDANFATPGAFNEA
                     ILTTDTCTKHIAVSLKIDGKTVTIGGSTKGSGMIHPNMATMLAFITTDASIESNTLHQ
                     LLKSSTDHTFNMITVDGDTSTNDMVLVMANHQVEHQILSQDHPQWETFVDAFNFVCTF
                     LAKAIARDGEGATKLISVNVSGAKSISDARKIGKTIVSSNLVKSAIFGEDANFGRIIT
                     AIGYSGCEIDPNCTYVQLNQIPVVDKGMAVLFDEQAMSNTLTHENVTIDVQLGLGNAA
                     ATAYGCDLSYDYVRINASYRT"
     gene            3598..4362
                     /gene="argB"
                     /locus_tag="POB55_000142"
     CDS             3598..4362
                     /gene="argB"
                     /locus_tag="POB55_000142"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HDI6499140.1"
                     /translation="MKFIVIKIGGSTLSDMHPSIINNIKHLRSNNIYPIIVHGGGPFI
                     NEALSNQQIEPHFVNGLRVTDKATMTITKHTLIADVNTALVAQFNQHQCSAIGLCGLD
                     AQLFEITSFDQQYGYVGVPTALNKDALQYLCTKFVPIINSIGFNNHDGEFYNINADTL
                     AYFIASSLKAPIYVLSNIAGVLINDVVIPQLPLVDIHQYIEHGDIYGGMIPKVLDAKN
                     AIENGCPKVIIASGNKPNIIESIYNNDFVGTTILNS"
     gene            4632..5111
                     /locus_tag="POB55_000143"
     CDS             4632..5111
                     /locus_tag="POB55_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HDI6499141.1"
                     /translation="MGIFIYAGIIGGLLSGIVKLGWEVMFPPRTPERNATNPPQELLQ
                     QLGFSSEFTHQTYTFSNMELPWVSFIVHFSFSIVIAIIYCILVKKYAYLAMGQGAVFG
                     IAIWVLFHLIIMPIMHTVPAVWDQPFQEHLSEFFGHIVWMMTIELVRQHFVYRYKLN"
     gene            complement(5439..6083)
                     /locus_tag="POB55_000144"
     CDS             complement(5439..6083)
                     /locus_tag="POB55_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HDI6499142.1"
                     /translation="MTVFVMQLQSNLKSIEELISQSRWSYKKPRTVNYRYNQDKLMHR
                     LGDILVQYGIQHDTGLLPHEWHYHISPRGKADIVQHNRDGQPIYVSLSYSYPYIVCVV
                     DKEPVGIDIEKISQRLDWRTLVTCFSTNEAHQICSLNDFYQIWTQKESFTKLIGEGLI
                     KGLDIYDMTQSHFYQSREVKFKQFIFDQFMLQVCFLGQAPWGYKKVSVFQLLSS"
     gene            complement(6096..13271)
                     /locus_tag="POB55_000145"
     CDS             complement(6096..13271)
                     /locus_tag="POB55_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HDI6499143.1"
                     /translation="MIMGNLRFQQEYFRIYKNNTESTTHRNAYWVKLAKNVEATKMMY
                     ALSTIVQQHASIRHFFDVTTDDNLTMILHEFLPFIEIKQVPSSSANYDLEAFFKQELS
                     TYHFNDSPLFKVKLFQFADAAYILLDFHVSIFDDSQIDIFLDDLCNAYRGNTVINNTR
                     QHAHINRNDDKDNQDASHIALDSNYFRLENNSDIHIDSYFPIKHPFEQALYQTYLIDD
                     MTSIDMASLAVSVYLANHIMSQQHDVTLGIHVPSHLPNDLHGNIVPLTLTIDAKDVCQ
                     RFTTDFNKCVLQNMSQLQCAKSSLSLETIFHCYHHMMSCCNDVIEDVHQIHDAHTSLA
                     DIEIFPHQHGFKIIYNSAAYDLLSIETLSDLVRNIYLQITEENGNKRTTVDELNLMTE
                     RDIQLYDDINLSLPEIDDAQTVVTLFEQQVEATPNHVAVQFDGVFITYQTLNARANDL
                     AHRLRNQYGVEPNDRVAVIAEKSIEMIIAMIGVLKAGGAYVPIDLNYPSDRQEYILKD
                     VTPKVVITYQALYENGKQNINHIDLNKIAWKNIDNLSKCNTLEDHAYVIYTSGTTGNP
                     KGTLIPHRGIVRLVHQNHYVPLNEETTILLSGTIAFDAATFEIYGALLNGGKLIVAKK
                     EQLLNPIAVEQLINENDVNTMWLTSSLFNQIASERIEVLVPLKYLLIGGEVLNAKWVD
                     LLNQKPKHPQIINGYGPTENTTFTTTYNIPNKVPNRIPIGKPILGTHVYIMQGERRCG
                     VGIPGELCTSGFGLAAGYLNQPELTADKFIKDSNINQLMYRSGDIVRLLPDGNIDYLY
                     RKDKQVKIRGFRIELSEVEHALERIQGINKAVVIVQNHDQDQYIVAYYEAMHTLSHNK
                     IKSQLRMTLPEYMIPVNFMHIEQIPITINGKLDKKALPIMDYVDTDAYVAPSTDTEHL
                     LCQIFADILHVNQVGIHDNFFELGGHSLKATLVVNRIEASTGKRLQIGDLLQKPTVFE
                     LAQAIAKVQEQNYEVIPETIVKDDYVLSSAQKRMYLLWKSNHKDTVYNVPFLWRLSSE
                     LNVAQLRQAVQRLIARHEILRTQYIVVDDEVRQRIVADVAVDFEEVNTHFTDEQEIMR
                     QFVAPFNLEKPSQIRVRYIRSPLHAYLFIDTHHIINDGMSNIQLMNDLNALYQHKLLL
                     PLKLQYKDYSEWMSHRDMTKHRQYWLSQFKDEVPILSLPTDYVRPNIKTTNGAMMSFT
                     MNQQMRQLLQKYVEKHQITDFMFFMSVVMTLLSRYARKDDVVVGSVMSARMHKGTEQM
                     LGMFANTLVYRGQPSPDKMWTQFLQEVKEMSLEAYEHQEYPFECLVNDLDQSHDASRN
                     PLFDVMLVLQNNETNHAHFGHSKLTHIQPKSVTAKFDLSFIIEEDRDDYTINIEYNTD
                     LYHSETVRHMGNQCMIMIDYILKHQDTLQICDIPNGTEELLNWVNTHVNDRMLNVPGN
                     KSIISYFNEVVSRQGNHVALVMNDLTMTYETLRNYVDAIAHMLLSNGVGNGQRVALFT
                     ERSFEMIAAMLATVKVGASYIPIDIDFPNKRQGAILEDAKVTAVMSYGVEIETTLPVI
                     QLENAKGFVESKENEQYDDLHGNQLENTAMLDNEMYAIYTSGTTGMPKGVAIRQRNLL
                     NLVHAWSTELQLGDNEVFLQHANIVFDASVMEIYCCLLNGHTLVIPDREERVNPEQLQ
                     QLINKHRVTVASIPLQMCSVMEDFYIEKLITGGATSTASFVKYIEKHCGTYFNAYGPS
                     ESTVITSYWSHQCGDLIPETIPIGKPLSNIQVYIMSDGLLCGIGMPGELCIAGDSLAI
                     GYINRPELMADKWQNNPFGKGKLYHSGDLARYTSDGQIEFLGRIDKQVKVNGYRIELD
                     EIENAILAIRGISDCVVTVSHFDTHDILNAYYVGEQQMEQDLKQYLNDHLPKYMIPKT
                     ITHIDYMPLTTNDKVDTTRLPNPSTIQQSNKVYSEPSNEIEQTFVDVFGEVLKQNDVG
                     VDDDFFELGGNSLEAMLVVSHLKRFGHHISMQTLYQYKTVRQIVNYMHQNQQSLVALP
                     DNLSELQKIVMSRYNLGVLEDSLSHRPIGNTLLTGATGFLGAYLIEALQGYSHRIYCF
                     IRADNEEIARYKLMTNLNDYFSEETVEMMLSNIEVIVGDFECMDDVVLPENMDTIIHA
                     GARTDHFGDDDEFEKVNVQGTVDVIRLAQQHHARLIYVSTISVGTYFDIDTEDVTFSE
                     ADVYKGQLLTSPYTRSKFYSELKVLEAVNNGLDGRIVRVGNLTSPYNGRWHMRNIKTN
                     RFSMVMNDLLQLDCIGVSMAEMPVDFSFVDTTARQIVALAQVNTPQIIYHVLSPNKMP
                     VKSLLECVKRKEIELVSDESFNEILQKQDMYETIGLTSVDREQQLAMIDTTLTLKIMN
                     HISEKWPTITNNWLYHWAQYIKTIFNK"
     gene            complement(13718..14968)
                     /locus_tag="POB55_000146"
     CDS             complement(13718..14968)
                     /locus_tag="POB55_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6499144.1"
                     /translation="MKRLSTTLKVRLISNFLQLIITTAFIPFIALYLTDMLSQSIVGI
                     YLVGLVVLKFPLSIISGYLIEIFPKKLLVLIYQATMVIMLVFMGVFGSHQLWQIIGFC
                     VAYAIFTIVWGLQFPVMDTLIMDAITEDVEHYIYKISYWMTNLSVAIGALLGGLMYGY
                     SMLLLFLIAACIFLIVLFILYIWLPQDRNQVKQSDDKRHASRYQKLQIMNIFRSYKLV
                     LKDRNYMLLISGFSIIMMGEFSISSYIAIRLKDQFETISIGSYDITGAKMLAILLMIN
                     TVVVILLTYSISKVVLKIDFKKALITGLLIYIVGYSGLTYLNQFGLLVVFMIIATVGE
                     IIYSPIVSEQRFKIIPKAKRGTYSAVNALGIHFSETLARLGIVLGVFLTSLQMGLYMF
                     IVLTIGASMLVAGVFGGQKQVNTN"
     gene            complement(15354..16379)
                     /locus_tag="POB55_000147"
     CDS             complement(15354..16379)
                     /locus_tag="POB55_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent formate dehydrogenase"
                     /protein_id="HDI6499145.1"
                     /translation="MKIVALFPEAVEGQENQLLNTKKALGLKTFLEERGHEFIILADN
                     GEDLDKHLPDMDVIISAPFYPAYMTRERIEKAPNLKLAITAGVGSDHVDLAAASEHNI
                     GVVEVTGSNTVSVAEHAVMDLLILLRNYEEGHRQSVEGEWNLSQVGNHAHELQHKTIG
                     IFGFGRIGQLVAERLAPFNVTLQHYDPINQQDHKLSKFVSFDELVSTSDAITIHAPLT
                     PETDNLFDKDVLSRMKKHSYLVNTARGKIVNRDALVEALASEHLQGYAGDVWYPQPAP
                     ADHPWRTMPRNAMTVHYSGMTLEAQKRIEDGVKDILERFFNHEPFQDKDIIVASGRIA
                     SKSYTAK"
     gene            complement(16554..16913)
                     /locus_tag="POB55_000148"
     CDS             complement(16554..16913)
                     /locus_tag="POB55_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2294 domain-containing protein"
                     /protein_id="HDI6499146.1"
                     /translation="MNTIDTHTKEQQFSNLVRSYRKEYVGKGPNSIRVSFKDNWAIAH
                     MTGVLSKVESFYLNDKRNESMLHYTRTEKIKQMYKEIDVNEMESLVGAKFVKLFTDID
                     LNDDEVISIFVFDKSIE"
     gene            complement(17130..18161)
                     /locus_tag="POB55_000149"
     CDS             complement(17130..18161)
                     /locus_tag="POB55_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HDI6499147.1"
                     /translation="MTLETLIKEQLDPHLVEVDEGTYYPRTFIQQLFVDGYFGEAALR
                     KNAEVIEAVSQSCLTTGFCLWCQLAFSTYLENATQPHLNNDLQQQLLSGEILGATGLS
                     NPMKSFNDLEKLNLEHTYVDGQLVVSGRMPAVSNIQEDHYFGAISKHESSDEFVMFIL
                     RANQDGITLVEKTNFLGVNGSATYQITLNQVVVPQSQIITHDAKQFAATIRPQFIAYQ
                     IPIGLGSIKSSLELIDAFSNAQNGINQYLEYDVEAFKKRYRQLREEYYAILDDGNLTS
                     HLNELISLKKDIGYLLLDVNQASVVNGGSRAYTPYSPQVRKLKEGFFFAALTPTLRHL
                     GKLEAELKG"
     gene            complement(18174..18935)
                     /locus_tag="POB55_000150"
     CDS             complement(18174..18935)
                     /locus_tag="POB55_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6499148.1"
                     /translation="MTRPTNNKFILPIITFIIFLGIWEMVIIIGHYQPVLLPGPALVG
                     KSIWTFIVTGEIFQHLAISLWRFVAGFVVALLVAIPLGFLLGRNRWLYNAIEPLFQLI
                     RPISPIAWAPFVVLWFGIGSLPAIAIIFIAAFFPIVFNTIKGVRDIEPQYLKIAANLN
                     LTGWSLYRNILFPGAFKQIMAGIHMAVGTSWIFLVSGEMIGAQSGLGFLIVDARNMLN
                     LEDVLAAIFFIGLFGFIIDRFISYIEQFILRRFGE"
     gene            complement(18932..19906)
                     /locus_tag="POB55_000151"
     CDS             complement(18932..19906)
                     /locus_tag="POB55_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDI6499149.1"
                     /translation="MKRLSIIVIIGIFIITGCDWQRTSKEPSKNAQNQQVIKIGYLPI
                     THSANLMMTKKLLSQYNHPKYKLELVKFNNWPDLMDALNSGRIDGASTLIELAMKSKQ
                     KGSNIKAVALGHHEGNVIMGQKGMHLNEFNNNGDDYHFGIPHRYSTHYLLLEELRKQL
                     KIKPGHFSYHEMSPAEMPAALSEHRITGYSVAEPFGALGEKLGKGKTLKHGDDVIPDA
                     YCCVLVLRGELLDQHKDVAQAFVQDYKKSGFKMNDRKQSVDIMTHHFKQSRDVLTQSA
                     AWTSYGDLTIKPSGYQEITTLVKQHHLFNPPAYDDFVEPSLYKEASRS"
     gene            complement(19920..20660)
                     /locus_tag="POB55_000152"
     CDS             complement(19920..20660)
                     /locus_tag="POB55_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6499150.1"
                     /translation="MIKIQQLQHHFGSHKVIHNFNLDISKGEIVTFIGKSGCGKSTLL
                     NIIGGFIHPSSGRVIIDNEIKQQPSPDCLMLFQHHNLLPWKTINDNIRIGFRQKISDE
                     EINAQLKLVDLEGRGKHFPEQLSGGMKQRVALCRAHVHKPNVILMDEPLGALDAFTRY
                     KLQDQLVQLKHKTQSTIILVTHDIDEAIYLSDRIVLLGEGCNIISQYEITASHPRSRN
                     DSHLLKVRNEIMETFALNHHQVEPEYYL"
     gene            complement(21002..21514)
                     /locus_tag="POB55_000153"
     CDS             complement(21002..21514)
                     /locus_tag="POB55_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HDI6499151.1"
                     /translation="MTLFLLEANNLDFASTKEELEAKAASLSTKTIPTLIEVQATENL
                     THGYFIVEANDEAEAKQFLTEADISIQLVKEVRLVGKDLDEVKNGDAHVDYLVTWNIP
                     EGITMDQYLARKKKNSVHYEEVPEVEFKRTYVCEDMSKCICLYNAPDEEAVRRARKAV
                     DTPIDGIEKL"
     gene            21692..21895
                     /locus_tag="POB55_000154"
     CDS             21692..21895
                     /locus_tag="POB55_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499152.1"
                     /translation="MDKESVVASLARNKKIAVETMAGQRYIIERILHTNDEKHIHILK
                     PKDVVLDVDSIKEIDENHLNDAT"
     gene            complement(22189..23148)
                     /locus_tag="POB55_000155"
     CDS             complement(22189..23148)
                     /locus_tag="POB55_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDI6499153.1"
                     /translation="MNGKKANTINRYKYFHHVNHQKIQQSSKKTLWASLIITLLFTVI
                     EFVGGLVSNSLALLSDSFHMLSDVLALGLSMLAIYFASKKPTARYTFGYLRFEILAAF
                     LNGLALIVISIWILYEAIVRIIYPQPIESGIMFMIASIGLLVNIILTIILVRSLKQED
                     NINIQSALWHFMGDLLNSIGVIVAVVLIYFTGWRIIDPIISIVISLIILRGGYKITRN
                     AWLILMESVPQHLDTDQIMADIKNIDGILDVHEFHLWSITTEHYSLSAHVVLDKKYEG
                     DDYQAIDQVSSLLKEKYGIAHSTLQIENLQLNPLDEPYFDKLT"
     gene            complement(23794..25281)
                     /locus_tag="POB55_000156"
     CDS             complement(23794..25281)
                     /locus_tag="POB55_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HDI6499154.1"
                     /translation="MAVNVRDYIAENYGLFINGEFVKGSSDETIEVTNPATGETLSHI
                     TRAKDKDVDHAVKVAQDAFESWSLTSKSERAQMLRDIGDKLMAQKDKIAMIETLNNGK
                     PIRETTAIDIPFAARHFHYFASVIETEEGTVNDIDKDTMSIVRHEPIGVVGAVVAWNF
                     PMLLAAWKIAPAIAAGNTIVIQPSSSTPLSLLEVAKIFQEVLPKGVVNILTGKGSESG
                     NAIFNHDGVDKLSFTGSTDVGYQVAEAAAKHLVPATLELGGKSANIILDDANLDLAVE
                     GIQLGILFNQGEVCSAGSRLLVHEKIYDQLVPRLQEAFSNIKVGDPQDEATQMGSQTG
                     KDQLDKIQSYIDAAKESDAQILAGGHRLTENGLDKGFFFEPTLIAVPDNHHKLAQEEI
                     FGPVLTVIKVKDDQEAIDIANDSEYGLAGGVFSQNITRALNIAKAVRTGRIWINTYNQ
                     VPEGAPFGGYKKSGIGRETYKGALSNYQQVKNIYIDTSNALKGLY"
     gene            25708..26091
                     /locus_tag="POB55_000157"
     CDS             25708..26091
                     /locus_tag="POB55_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HDI6499155.1"
                     /translation="MRLILIVIGLIFTALGIAGAVLPLLPTTPFLLVAVFCFARSSDR
                     FYNWLINQKIYKEYVENFYLHRGYTLQQKIKILISLYIVIGFSIYMVDVLAVRIGLII
                     MVIIQTAVLFTFVKTLPKSNHKIEE"
     gene            26098..26424
                     /gene="isdI"
                     /locus_tag="POB55_000158"
     CDS             26098..26424
                     /gene="isdI"
                     /locus_tag="POB55_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylobilin-forming heme oxygenase IsdI"
                     /protein_id="HDI6499156.1"
                     /translation="MFMAENRLQLQKGSAEETIERFYNRQGIETIEGFQQMFVTKTLN
                     TEDTDEVKILTIWESEDSFNNWLNSDVFKEAHKNVRLKSDDDGQQSPILSNKVFKYDI
                     GYHYQK"
     gene            complement(26489..27634)
                     /gene="cap8P"
                     /locus_tag="POB55_000159"
     CDS             complement(26489..27634)
                     /gene="cap8P"
                     /locus_tag="POB55_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8P"
                     /protein_id="HDI6499157.1"
                     /translation="MKKIMVIFGTRPEAIKMAPLVKEIDHNGNFEANIVITAQHRDML
                     DSVLSIFDIQADHDLNIMQDQQTLADLTANALAKLDSIINEEQPDMILVHGDTTTTFV
                     GSLAAFYHQIPVGHVEAGLRTHQKYSPFPEELNRVMVSNIAELNFAPTVIAAKNLLFE
                     NKDKERIFITGNTVIDALSTTVQNDFVSTIINKHKGKKVILLTAHRRENIGEPMHQIF
                     KAVRDLADEYKDVVFIYPMHRNPKVRAIAEKYLSGRNRIELIEPLDAIEFHNFTNQSY
                     LVLTDSGGIQEEAPTFGKPVLVLRNHTERPEGVEAGTSRVIGTDYDNIVRNVKQLIED
                     DEAYQRMSQANNPYGDGQASRRICEAIEYYFGLRTDKPDEFVPLRHK"
     gene            complement(27711..28973)
                     /gene="cap8O"
                     /locus_tag="POB55_000160"
     CDS             complement(27711..28973)
                     /gene="cap8O"
                     /locus_tag="POB55_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8O"
                     /protein_id="HDI6499158.1"
                     /translation="MKLTVVGLGYIGLPTSIMFAKHGVDVLGVDINQQTIDKLQSGQI
                     SIEEPGLQEIYEEVLSSGKLKVSTTPEASDVFIIAVPTPNNDDQYRSCDISLVMRALD
                     SILPFLKKGNTIIVESTIAPKTMDDFVKPVIENLGFTIGEDIYLVHCPERVLPGKILE
                     ELVHNNRIIGGVTKACIEAGKRVYRTFVQGEMIETDARTAEMSKLMENTYRDVNIALA
                     NELTKICNNLNINVLDVIEMANKHPRVNIHQPGPGVGGHCLAVDPYFIIAKDPENAKL
                     IQTGREINNSMPAYVVDTTKQIIKALSGNKVTVFGLTYKGDVDDIRESPAFDIYELLN
                     QEPDIEVCAYDPHVELDFVEHDMSHAVKDASLVLILSDHSEFKNLSDSHFDKMKHKVI
                     FDTKNVVKSSFEDVSYYNYGNIFNFIDK"
     gene            complement(29027..29914)
                     /gene="capN"
                     /locus_tag="POB55_000161"
     CDS             complement(29027..29914)
                     /gene="capN"
                     /locus_tag="POB55_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide type 5/8 biosynthesis
                     epimerase CapN"
                     /protein_id="HDI6499159.1"
                     /translation="MRKNILITGVHGYIGNALKDKLIEQGHQVDQINVRNQLWKSTSF
                     KDYDVLIHTAALVHNNSPQARLSDYMQVNMLLTKQLAQKAKSEDVKQFIFMSTMAVYG
                     KEGQVGKSDQIDTQTPMNPTTNYGISKKFAEQALQELISDSFKVAIVRPPMIYGAHCP
                     GNFQRLMQLSKRLPIIPNINNQRSALYIKHLTAFIDQLISLEVTGVYHPQDSFYFDTS
                     SVMYEIRRQSHRKTVLINMPSVLNKYFNKLSVFRKLFGNLIYSNTLYENNNALEVIPG
                     KMSLVIADIMDETTTKDKA"
     gene            complement(29914..30471)
                     /gene="cap8M"
                     /locus_tag="POB55_000162"
     CDS             complement(29914..30471)
                     /gene="cap8M"
                     /locus_tag="POB55_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8M"
                     /protein_id="HDI6499160.1"
                     /translation="MKRLFDVVSSIYGLVVLSPILLITALLIKMESPGPAIFKQKRPT
                     INNELFNIYKFRSMKIDTPNVATDLMDSTSYITKTGKVIRKTSIDELPQLLNVLKGEM
                     SIVGPRPALYNQYELIEKRTKVNVHTIRPGVTGLAQVMGRDDITDDQKVAYDHYYLTH
                     QSMMLDMYIIYKTIKNIVTSEGVHH"
     gene            complement(30482..31687)
                     /gene="cap8L"
                     /locus_tag="POB55_000163"
     CDS             complement(30482..31687)
                     /gene="cap8L"
                     /locus_tag="POB55_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8L"
                     /protein_id="HDI6499161.1"
                     /translation="MSEKKILILCQYFYPEYVSSATLPTQLAEDLIANHINVDVMCGW
                     PYEYSNHKQVSKTEMHRGICIRRLKYSRFNNKSKVGRIINFFSLFSKFVINIPKMLKY
                     DQILVYSNPPILPLIPDVLHRLLKKKYSFVVYDIAPDNAIKTGATRPGSMIDKLMRYI
                     NRHVYKNAENVIVLGTEMKNYLLNHQISKNADNIHVIPNWYDMRQLQDNRIYNDTFKA
                     YREQYDKILLYSGNMGQLQDMETLISFLKLNKDQPQTLTILCGHGKKFADVKTAIEDH
                     RIENVKMFEFLTGTDYADVLKIADVCIASLIKEGVGLGVPSKNYGYLAAKKALVLIMD
                     KQSDIVQHVEQYDAGIQIDNGDAHAIYNFINTHSSKELHEMGERAHQLFKDKYTREIN
                     TMKYYNLLK"
     gene            complement(31721..32959)
                     /gene="cap8K"
                     /locus_tag="POB55_000164"
     CDS             complement(31721..32959)
                     /gene="cap8K"
                     /locus_tag="POB55_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8K"
                     /protein_id="HDI6499162.1"
                     /translation="MRLNKFIGDSFLMILSSGIAQVILIITTPIITRLYSPTEFGEFT
                     IFSNIAMILIPIINARYDLLIVNTKNDRSANILSQISFLISLLILLILIPIFAISACL
                     YPNFILDFIFIIIMLFLVSLTNIFTNYLNKERKYKVLSLINVFRAGSMALLQIIFGLL
                     ALGSLGLIIGFSLSYIAGITLGYKTFKKHFNIVRDKEETKALFLENKNQLVYSTPSIL
                     LNSLSFSVVVFFIGILYTNTEVGIYGMAIRVLGIPVTIISLGLSKIFMQQANDYYIEH
                     GNFRNLLLKFSSILVIVSIILYVPLYLFSEELVNILLGHSWVDAITVIKIVIPLFVIR
                     LIVSTVSLSVIVLQKQQLELILQALFLIGTTATFVISKMLNLTFLNFVSINTIVLIVS
                     YMIFFIALYYFAKNKQFKNS"
     gene            complement(32968..33525)
                     /gene="cap8J"
                     /locus_tag="POB55_000165"
     CDS             complement(32968..33525)
                     /gene="cap8J"
                     /locus_tag="POB55_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8J"
                     /protein_id="HDI6499163.1"
                     /translation="MIVKTFMKSKIFRLMNTPLLLFYKKEYLTGYYFENKVAGWLWAW
                     KAVPFKLLGINTSLPFPADITVRMHNPNNIVFDKNDIHIFQSPGTYFNNFSAVIYIGR
                     GVYIAPNVGIITANHNIKNLKSHAPGEDVKIGNYSWIGMNSVILPGVELGEHTIVGAG
                     SVVTKSFPEGNVVIGGNPAKIIKKI"
     gene            complement(33522..34916)
                     /gene="cap8I"
                     /locus_tag="POB55_000166"
     CDS             complement(33522..34916)
                     /gene="cap8I"
                     /locus_tag="POB55_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8I"
                     /protein_id="HDI6499164.1"
                     /translation="MNKIYNVTSYVIAILMFPCLMLGDKPLLFLAPISYGVGKLFISF
                     SNNPNFKFSKIVYDVLGFLRLVFIPAMIVFFQDSTIDNLPLGQAYFNQAVIYMSVEFI
                     IGSLFILILSKLFKHEVVSRNSFTLSGSSIYYIVFGLVICGIFVAFPEVRKNISFLII
                     KTDAMGRGTEATSGLNVLFVMLFQLALALLFLIIAYASYKKYKENPKIIYVVLPLAIG
                     ILNISLIVGERRSYQLYTMVAVLTVVSILFSKHKRRINIIIISVGIFVLALMTLYKEL
                     YVFNYSSYSEALNSTSVSNLKIVDTLQSYFYGPSNIAASIDYLNYYNGSFKQYLFDNT
                     RAVFGFNFFLDKKQLITSQLFNQLIYGSKQLTGHLISSAGYGIIYFGPLFFYLNLIAN
                     IFFAFLSEYIIRKSHSLEVIFIGTYIYMRLITSIFSHPTPLITLISMILVVYVIAIIP
                     GIIIKKFTKKVGIE"
     gene            complement(34909..>35613)
                     /gene="cap8H"
                     /locus_tag="POB55_000167"
     CDS             complement(34909..>35613)
                     /gene="cap8H"
                     /locus_tag="POB55_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8H"
                     /protein_id="HDI6499165.1"
                     /translation="KLIKYRMKKIVDASRVTTLSSEGFLKFLPKSEKYRIIYSYNMSI
                     IRESNVTDGFKKRWPINIGFIGNVRFNEINQKLIKELANDSRFHMQYFGTGSEKLEVF
                     ARENFINNITFSGGFDLKETPKYLNEIDILNNLFGNQNIALDTALSIRMYYALFLNKP
                     IITTDDTFTATEANKFGLGFSINPENLKGIGDELMDWYNNLDVMDINHKREAYRNDVI
                     ENNKQFYQEIGRIFNE"
BASE COUNT        11634 a         6692 c         5288 g        11999 t
ORIGIN      
        1 gacacttata ttgaacaact gtaatatatt aatattaatt ctttaaaatg tataaatata
       61 aaggagggag accgatgaat tcaatcattg aattaactga ttattatagc tctaataatt
      121 atgcaccact taagcttgtc atttctaaag gtaaaggtgt caaagtttgg gatactgatg
      181 gcaaacaata tatagattgc atttcgggtt tttcagttgc aaaccaaggc cattgtcatc
      241 caacaattgt taaagcgatg acagaacaag cttcaaagtt gtctatcatt tcacgtgtcc
      301 tttatagtga caatctcggg aaatgggaag aaaaaatttg tcatcttgct aagaaagaca
      361 aagtactccc ccttaactct ggtactgaag ctgttgaagc agccattaaa attgctagaa
      421 aatggggctc tgaagttaaa ggcattactg acggacaagt tgaaatcatc gctatgaata
      481 acaattttca cggtcgtaca cttggctcat tatcactatc taaccacgac gcatataaag
      541 caggatttca ccccctactt caaggcacta caacagtaga ttttggagac attgaacaat
      601 taacacaagc tatttcaccg aatacagcag caattatttt ggaaccaatt caaggtgaag
      661 gtggcgttaa tataccaccg aaaggatata ttcaagctgt gcgtcaacta tgtgataaac
      721 atcaaatatt attgattgca gatgaaattc aagttggtct tggtagaact gggaaatggt
      781 ttgctatgga atgggagcaa gtcgttccag acatttatat tttaggtaag gcattgggtg
      841 gcggcttata ccctgtatct gctgtacttg caaataatga tgtcatgcgt gttctaacac
      901 caggtacaca tggttcaaca tttggtggta accctttagc cattgcaata tcgacggcag
      961 cgcttgatgt acttaaagat gaacaactgg ttgaacgatc agaacgctta ggttcatttt
     1021 tattaaaagc gttgctacaa cttaaacatc ctagtattaa agaaattaga ggtcgtggtt
     1081 tatttatagg catagagctt aacacagatg ctgcaccttt tgtggatcaa ctgattcaac
     1141 gtggaatctt atgcaaagac acgcatcgta ctatcattcg attgtctcca cctctagtca
     1201 ttgataaaga ggaaatccat caaattgttg cagcttttca agacgttttt aaaaattaac
     1261 aattaatcat ttatatatga cataggaggg attcatgatg attaaagtag gtatcgttgg
     1321 cggtagcggt tatggcgcaa ttgaattaat tcgattgtta caaacacatc ctcatgtaac
     1381 gattgcacac atctactcac attcaaaagt agatgaaccg ttgaaattaa catttccaca
     1441 tttacaacat attatgcaac atttcgaagc acttacagtg gacaataatg actgtgatgt
     1501 aattttcttt gcgacaccag cacccgtaag taaaacatgt atccctccct tagtagaaaa
     1561 aggtattcat gttatcgatt tatctggcgc atttagaatt aagaatcgtg aaatatatga
     1621 agcatattac aaagaaactg ctgcagcaca agatgatttg aatcatgcta tttacagcat
     1681 ttcagaatgg caatcgtttg ataacaatgg aacgaagctc atttctaatc ctggctgttt
     1741 ccctacagca acattattag cattacatcc acttattagc gaaaaaatag tagatttgtc
     1801 atctattatt attgatgcta agaccggcgt gtcaggtgct ggtcgttcat tatcacaacg
     1861 tgttcatttt tcagaaatga atgaaaatct aagcgcttat gcaatcggaa accataaaca
     1921 caaaccggaa atcgagcaat atttatctat cattgcgggt caagatgtat cagtcatatt
     1981 tacaccacat ctcgtaccaa tgacacgagg tattttatca acaatatatg tcaaattatc
     2041 atctgaatat acgactgaat cattacataa attaatgacc tcttattatg ctaatcagcc
     2101 atttgtcaga attagagata ttgggacttt tccaaccaca aaagaagtac tcggtagtaa
     2161 ctactgcgat atcggcatct atgtagatga aacaacgcaa acagcaattt tagtatcagt
     2221 gattgataac cttgtcaaag gcgcaagtgg gcaagccatt caaaatttaa atatattata
     2281 tgattttgaa gtgacgactg gcctaaatca atcaccagtt tatccataag gggtgttaga
     2341 atgaaacatc aagaaacgac atcacaacaa tataactttt caattattaa acatggcgat
     2401 atcagtacac ctcaaggctt cacggctggt ggtatgcaca tcggtttacg cgctaacaaa
     2461 aaagactttg ggtggattta ctcatcgtct ttggcaagtg cagctgccgt atatacttta
     2521 aatcagttta aagctgcacc acttattgtc actgaagaca ctttacaaaa gtctaaagga
     2581 aaattacaag cacttgttgt taattcagct aatgcaaatt cttgtaccgg tcaacaaggc
     2641 atagatgatg cacgacaaac acaaacatgg gttgctcaac aacttcaaat accatctgag
     2701 catgttgctg ttgcttcaac tggggtcatt ggtgaatatt tgcctatgga taaaattaag
     2761 actgggaccg aacatattaa ggatgctaat tttgcaacgc caggtgcgtt taacgaggca
     2821 attttaacaa ctgatacctg tacaaaacat atcgctgtat cactaaaaat cgatggtaaa
     2881 accgttacaa ttggtggtag caccaaaggt tcaggtatga ttcacccaaa tatggctacc
     2941 atgcttgctt ttataacaac cgatgcatcg attgaatcga atacacttca tcaattatta
     3001 aaatcttcga ctgaccatac atttaatatg attactgttg atggcgatac aagtacaaat
     3061 gacatggtat tagtcatggc aaatcaccaa gttgaacacc aaatacttag tcaagaccat
     3121 ccacaatggg aaacatttgt tgatgcattc aattttgtct gtacattttt agctaaagct
     3181 atagccagag atggcgaagg cgcaacaaag ttaatcagcg tcaatgtgtc aggcgctaaa
     3241 tcaatatcag atgcaagaaa aatcggtaaa actatcgtaa gttcaaatct agtaaaatca
     3301 gctatttttg gcgaagatgc caattttggt cgaatcatta cagctattgg ctacagcgga
     3361 tgtgaaattg atcctaactg cacatatgtt caactgaacc aaatacctgt cgttgataaa
     3421 ggtatggctg tactatttga tgagcaagct atgtcgaata cattaactca tgaaaatgtc
     3481 acaattgacg ttcagcttgg tttaggtaac gctgcagcga ctgcatacgg ttgtgattta
     3541 tcctatgatt atgtgcgtat caacgcatca tatcgaacat aaggtggtgt tggttagatg
     3601 aaatttattg tcattaaaat tggtggcagt acacttagtg acatgcatcc atcaattatt
     3661 aacaacatta agcatttacg atcaaacaac atctacccca ttatcgttca tggcggtggc
     3721 ccatttatta atgaagcatt atcaaaccag caaatcgagc cacactttgt taatggccta
     3781 agagtgactg ataaagcaac catgaccatt actaaacaca cgctcattgc agacgttaac
     3841 actgcattag tagctcaatt taaccagcac caatgttctg caataggctt atgtggtttg
     3901 gatgcacagc tgtttgaaat tacatctttt gatcaacaat atggatatgt cggtgttccg
     3961 accgctttaa ataaggatgc tttacagtat ttatgtacta aatttgtacc tatcatcaat
     4021 tcgattggtt tcaataacca tgatggagaa ttttacaata ttaatgctga cacgcttgcc
     4081 tattttattg catcatcatt aaaagcgcct atttatgtat taagtaatat tgcaggtgta
     4141 ctcatcaatg atgttgttat acctcaattg ccattagtcg atattcatca atatattgaa
     4201 catggtgata tttatggagg tatgattccc aaagtgctag atgccaaaaa tgcgattgaa
     4261 aatggctgtc ctaaagttat cattgcatca ggaaacaagc caaatatcat tgaatctatt
     4321 tacaataatg attttgttgg cacaacaatc cttaattcat aactatgaaa ttaaggccta
     4381 acaagttttg acacgcgaga tgattccagt tcgattatcc attgcgctaa aacatttatt
     4441 taccgttcat ctcgttaaca attttgaata cagtacgata caatatgaga tgtaaaaaac
     4501 taataacctt ttacaaattt gtttatcaaa atattttaag ttctgcaaag ctttttattg
     4561 tgattatttt cacaaaatac tataatgagg atagtaaata gagaggagtc cttaagttga
     4621 cgaaacgaca aatgggtata ttcatttatg ctggaattat cggtggcttg ttatctggaa
     4681 ttgtaaaatt aggttgggag gtcatgtttc cacctcgcac accagaacgt aatgcaacga
     4741 acccacctca agagttattg caacaattag gatttagtag tgagtttacg catcaaacat
     4801 atacattttc aaatatggaa ttgccttggg taagctttat tgtccacttt agtttttcta
     4861 tcgtcattgc aattatttac tgcatattag ttaaaaaata cgcttactta gcaatgggac
     4921 aaggtgctgt ttttggtatt gctatttggg tattattcca ccttatcatt atgccaatca
     4981 tgcatactgt acctgctgtg tgggatcaac cattccaaga gcatctgtca gaattctttg
     5041 gccatatcgt ctggatgatg acaattgaat tagtgcgaca acattttgtc tatcgctata
     5101 aattaaatta atacactgac taacattaac gtgagtttta aatcatcgtt tgagtatgat
     5161 gattgatgct cacgttattt tattaactga catgatatga ttccagccaa cttacgtgag
     5221 cattaaagtc tcaaatgcgt cgtaacaaac tattattttc ggtaatttca atattgctca
     5281 gtatattttt accttatcac ttactttaat ctcgtcatga ttttgaatga tgccatcgtg
     5341 tattcacctt tcatttttcc aataaaaaaa catctaacag taaacattta ggcagtatag
     5401 tttaaaatca ctgcgcaatg atactgtcag acgtcatatt aactactcaa taactgaaat
     5461 acagacactt ttttataccc ccagggtgcc tgtcctaaga aacatacctg tagcataaac
     5521 tgatcaaaaa taaattgttt gaacttcact tcacgtgatt gataaaagtg tgattgtgtc
     5581 atatcataaa tgtccaatcc tttgattaaa ccttcaccaa tcaattttgt aaaactttct
     5641 ttttgtgtcc atatttgata aaaatcattt aaactacata tttgatgtgc ttcgtttgta
     5701 gagaaacacg tcactaacgt acgccagtct aaacgttgtg atatcttttc gatatcaata
     5761 ccaactggtt ctttatcgac aacacacacg atataaggat aactatatga taagctcaca
     5821 tagatgggct gtccatcacg attgtgttga acaatatctg ccttacctcg tggcgaaatg
     5881 tgataatgcc attcatgtgg taataaacct gtgtcatgtt gaattccata ttgcactaaa
     5941 atatctccca atctgtgcat gagtttatct tgattgtatc tatagttgac tgtacgcggt
     6001 tttttatatg accaacgact ttgtgatatt aattcttcaa tacttttcaa gttactctgt
     6061 aattgcatta caaatactgt cataactttc cctacttact tattgaatat tgttttgata
     6121 tattgtgccc aatgatacag ccaattgtta gttatcgttg gccatttttc actgatgtga
     6181 ttcattattt ttaatgttaa tgttgtatct atcattgcta gttgttgttc acggtcaaca
     6241 ctagttaatc caatcgtttc gtacatgtct tgtttctgta aaatttcatt aaatgattca
     6301 tcgctgacga gttcaatttc tttgcgctta acgcattcta acaaagattt caccggcatt
     6361 ttattaggtg atagcacatg gtaaatgatt tgtggtgtgt tgacctgtgc taatgcgaca
     6421 atttgtcttg cagtcgtatc cacaaaagaa aaatctacag gcatttcagc catgctaacc
     6481 ccgatacaat ccagttgtaa caaatcattc attaccattg aaaaacggtt agtctttata
     6541 tttctcatat gccatcttcc attgtaagga ctcgtcaaat taccaacacg tacaatccga
     6601 ccatctaagc cattatttac agcttctaat acttttaatt cactataaaa tttgctccgt
     6661 gtatatggtg atgttagtag ttgcccttta tagacatccg cttctgaaaa tgtcacatct
     6721 tctgtgtcta tatcaaaata agttcccaca cttatcgtag acacatatat taaccttgca
     6781 tgatgttgtt gtgccaaacg tatgacatca acagtacctt gaacatttac tttttcaaat
     6841 tcatcatcat caccaaagtg atctgtacga gcacctgcat gaataatcgt atccatgttt
     6901 tctggtaaaa caacatcatc catacactcg aaatcaccaa caatgacttc aatgtttgat
     6961 aacatcattt caaccgtctc ttctgaaaaa taatcattta aattcgtcat caacttatac
     7021 cttgctattt cctcattatc agcacgtatg aaacaataaa tgcgatgact gtatccttgt
     7081 agtgcttcaa tcagataagc acctaaaaac cctgtcgcgc cagtcaatag tgtatttcct
     7141 ataggtcgat gacttagact atcctctaaa acacccaaat tataacgaga cataacaatc
     7201 ttttgtaatt ccgaaagatt atccggtaat gcaactaatg attgttgatt ttggtgcata
     7261 taattaacaa tctgtcgcac ggttttatat tggtataatg tctgcattga aatatgatgg
     7321 ccaaatcgtt ttaaatgcga gacaactaac atcgcctcta atgagttacc accaagttca
     7381 aagaaatcat cgtcaacacc gacatcattt tgtttcaata cctctccgaa tacatcaaca
     7441 aatgtctgct caatttcatt agatggttcg ctatacactt tattagactg ttgtatagtt
     7501 gatggatttg gcaaacgcgt agtatccacc ttgtcattcg tggttaatgg catataatcg
     7561 atatgcgtta tagtcttagg aatcatatac ttaggcagat gatcatttaa atattgcttc
     7621 aaatcctgct ccatttgttg ctctccgaca taataagcat tcaatatatc atgcgtatca
     7681 aagtgactta ctgtaacaac acaatcagat ataccacgaa tagctaatat tgcattttca
     7741 atttcatcaa gttcaatacg gtacccgtta actttcactt gtttatctat tcttcctaaa
     7801 aattcaattt gaccatcaga tgtataacgt gctaaatcac cactatgata caactttcct
     7861 ttaccaaatg gattattttg ccatttatca gccattaatt ctggacggtt aatatatcct
     7921 atcgctaaac tatcacctgc aatacacaac tcgcctggca taccaatacc gcataacaaa
     7981 ccatctgaca taatatacac ttggatgtta gataagggtt tgccaattgg aatcgtctca
     8041 ggtatcaaat caccacattg atgtgaccaa tacgatgtga tgactgttga ctcagatggt
     8101 ccataggcat tgaaatacgt gccacaatgc ttctcaatat atttaacaaa ggatgccgta
     8161 ctagttgccc cgcctgtaat caacttttca atataaaagt cttccataac actacacatc
     8221 tgtaacggaa tcgacgcaac cgtcacacga tgcttattaa tgagttgttg taactgttct
     8281 ggattaacac gttcctctct atctggaatc acaagcgtat gaccatttaa caaacaacaa
     8341 taaatctcca taactgatgc atcaaaaaca atatttgcat gttgcaaaaa tacttcattg
     8401 tcgcctaatt gcaattcagt tgaccatgca tgcactaaat tcaacaaatt tcgttgtcgt
     8461 atggcaaccc ctttaggcat cccggtcgta ccagatgtgt aaatagcata catctcatta
     8521 tctaacatcg ctgtgttttc aagttgattg ccatgtaaat catcatattg ttcattttcc
     8581 tttgattcaa caaagccttt agcattttcc aattgaatga ctggtaatgt cgtttcaatt
     8641 tcaacgccgt aagacatgac tgcagttact ttagcatcct ccaaaattgc accttgtcgt
     8701 ttattcggaa aatcaatatc gataggtata taagatgcac ctactttaac tgtcgccaac
     8761 atcgccgcaa tcatttcaaa actacgttct gtaaacaagg caacccgttg accattgccc
     8821 acaccatttg ataggagcat gtgcgcaatg gcatccacat agttgcgtaa tgtttcatac
     8881 gtcattgtca aatcattcat gactagcgca acatgattac cttgtcgtga gacaacttca
     8941 ttaaagtaac ttatgataga tttatttccc gggacattaa gcattcgatc gttaacatgc
     9001 gtattgaccc aatttagaag ttcctccgtg ccgtttggta tatcacaaat ttgtagtgta
     9061 tcttgatgct tcaaaatata atcaatcata atcatacatt gattacccat gtgacgaact
     9121 gtttctgagt gatataaatc ggtattatac tcgatattga ttgtatagtc atcgcgatct
     9181 tcttcaatga tgaaagataa atcaaatttc gccgtcactg atttgggttg aatgtgtgtt
     9241 aatttactat gcccaaaatg agcatgattc gtttcattgt tttgtagtac taacatgaca
     9301 tcaaataatg gattccgtga ggcatcatgt gattgatcta agtcatttac taaacattcg
     9361 aatgggtatt cttgatgctc gtatgcctcc aaactcattt ccttaacctc ttgtaaaaac
     9421 tgtgtccaca ttttatcagg tgacggttgc cctctatata ccaacgtatt agcaaacatg
     9481 cctagcattt gctccgtgcc tttatgcata cgcgcactca tcacactacc gacaacaaca
     9541 tcatcttttc gagcatatct acttaacaac gtcatgacca cactcataaa gaacataaaa
     9601 tcagtaattt gatgcttttc tacatacttt tgaagtagct gtctcatttg ttgattcatt
     9661 gtaaatgaca tcattgctcc atttgtcgtt ttaatatttg gtctaacata gtctgtcggt
     9721 aagcttaaaa taggtacttc atctttgaat tgagataacc aatattgtct atgtttcgtc
     9781 atatcacgat gcgacatcca ctcactatag tctttatatt gcaatttaag tggtaacaat
     9841 aatttatgtt gataaagtgc gttaagatca ttcattaatt gtatattact cataccgtca
     9901 ttaatgatat gatgcgtatc tataaagagg tatgcatgta agggacttct aatgtatctc
     9961 actctaattt gacttggctt ttccaaatta aaaggtgcta caaattggcg catgatttct
    10021 tgttcatccg taaaatgcgt gttaacttct tcaaagtcaa ctgcaacatc tgccacaata
    10081 cgttgtcgaa cctcatcatc tacaacaata tattgtgttc gtaaaatctc atgtcgcgct
    10141 atcaaacgct gcactgcttg tcgcaattga gctacattaa gttctgatga taaccgccat
    10201 aaaaaaggta cgttatacac cgtatcttta tggtttgatt tccataataa atacatacgc
    10261 ttttgtgcag agctcagcac ataatcatct ttaactatag tttctggaat cacttcatag
    10321 ttttgttctt gaaccttagc aatcgcttgt gctagttcaa atacagttgg cttttgtaat
    10381 aaatcaccaa tttgtaatcg tttcccagta gatgcctcta tccgattcac cactaacgtt
    10441 gcttttaatg aatggccacc taattcaaag aaattatcat gaatacctac ttgattcaca
    10501 tgtaaaatat ctgcaaaaat ttggcatagc aagtgttcgg tatctgtact cggtgctaca
    10561 taggcatccg tatcgacata gtccatgata ggcaatgcct tcttatctaa tttcccatta
    10621 atagtaatag gaatttgctc aatatgcatg aaattaactg gtatcatgta ctccggtaag
    10681 gtcatacgta attgtgattt aatcttatta tgtgataatg tatgcatcgc ttcataataa
    10741 gcaacgatat actgatcttg atcatgattt tgaacaataa caactgcttt attaatacct
    10801 tgtatacgct cgagcgcatg ctcaacctct gacaactcaa tcctaaaccc tcgaatctta
    10861 acttgtttgt cctttcgata taaataatct atgttgccat cgggtaacaa acgaacgata
    10921 tcaccacttc tatacatcag ctgatttata tttgaatctt tgataaattt atctgctgtc
    10981 aattctggct gatttaaata acctgcagct aacccaaagc cacttgtaca taattctcca
    11041 ggaataccaa cgccacaccg acgctcgcct tgcatgatat aaacatgagt acccagaatc
    11101 ggtttaccaa taggaatacg atttggaact ttgttaggta tattatacgt cgttgtaaat
    11161 gttgtatttt cagttggtcc ataaccatta ataatttgag gatgcttcgg tttttgatta
    11221 agcaaatcca cccacttagc attcaatact tctccaccaa ttaataaata ctttaacggt
    11281 accaatactt ctattcgttc actagcaatc tgattaaata atgaggaggt taaccacata
    11341 gtattaacgt cattttcatt gattaattgt tctaccgcta ttggatttaa taattgttct
    11401 tttttagcaa caatcagctt tccaccattg agcaatgcac catatatttc aaatgttgca
    11461 gcatcaaagg ctatagttcc tgataacaaa atcgtcgtct cttcatttaa tggtacataa
    11521 tgattttgat ggaccaagcg aacaatacct cggtgcggaa ttagtgtccc tttagggtta
    11581 ccagttgtcc ccgacgtgta aataacataa gcatgatctt ctaacgtgtt acatttagaa
    11641 agattatcaa tatttttcca cgctatctta ttcaaatcaa tgtgattaat attttgttta
    11701 ccattttcat ataaagcttg gtacgttatt acaactttag gcgttacatc ttttaaaatg
    11761 tactcctgac gatcacttgg atagttcaga tcaattggca cgtaagcccc accagctttc
    11821 aacacaccta tcatcgctat tatcatctca atactttttt cagctatgac agcgacacga
    11881 tcattaggtt caacaccata ctggtttctc aaacggtgtg ctaaatcatt cgcgcgtgca
    11941 ttcaatgttt gatatgttat aaacactccg tcaaattgca cagcgacatg attcggcgtt
    12001 gcttcaactt gttgctcaaa taaggtaaca actgtttgcg catcatctat ctcaggcaaa
    12061 cttaaattga tatcgtcata taattgaata tcacgttctg tcatcaaatt aagttcatct
    12121 acagttgttc gtttatttcc attttcttca gtaatttgca aataaatatt tcgaactaag
    12181 tcactcagcg tctcgattga gagcaaatca tatgctgcac tgttatatat aattttgaac
    12241 ccgtgttgat gtggaaaaat ttcaatatcc gctaaagatg tatgtgcatc atgtatttga
    12301 tgtacatcct caataacatc attacaacaa gacatcatat gatgataaca atgaaaaata
    12361 gtctctagtg aaagcgaaga cttcgcgcac tgtaattgcg acatattttg caacacacat
    12421 ttattaaaat ctgttgtaaa acgttgacat acatcttttg catcgattgt taacgttaac
    12481 ggcacaatat ttccgtgtaa atcatttggt aaatgtgatg gtacatgtat acctaatgtg
    12541 acatcatgtt gttgactcat tatatgatta gctaaataca cactaacagc caacgatgcc
    12601 atatctattg atgtcatatc atcaatcaaa tacgtttgat ataaagcttg ttcaaatgga
    12661 tgcttaattg gaaaataact atcaatatgg atgtcagagt tattctctaa ccgaaaatag
    12721 tttgagtcta atgctatatg cgatgcatct tgattgtctt tatcatcatt tctatttata
    12781 tgtgcatgct gtcgagtatt gttaataaca gtattgccac gatatgcatt gcataaatca
    12841 tcaagaaaaa tatcaatttg actatcatcg aaaatggaca catgaaaatc taatagtata
    12901 tatgcagcat cagcgaactg aaacaattta actttgaata aaggtgaatc attaaaatgg
    12961 taagtactta attcttgctt aaaaaaagct tctaaatcat agtttgcgga agaagatgga
    13021 acttgtttta tctcaataaa aggcagaaat tcatgaagta tcattgttaa attgtcatcg
    13081 gtagtaacat caaaaaaatg tcttatagat gcatgttgtt gcacaattgt cgataatgca
    13141 tacatcattt tagtagcttc aacattttta gcgagtttaa cccaatacgc attacggtgt
    13201 gtcgttgatt ctgtattatt tttgtatata cgaaaatatt cctgttgaaa tctcaaatta
    13261 cccataatca taaaaagtcc ttctttcata tcataatact cattacttac tgaaattgca
    13321 tgatgatatg ataaccgacg aaatgttaat taactcgtta tgtaatgatt aatataaaac
    13381 accattcgca acatatgagc gatatattct accctaaaat acatcttgta tcatcgttac
    13441 aattggtata tttttcaatg taaattacat acatcttcga taaatagcac actacaactc
    13501 gttaatcact ttctgttgtt cacatctcat tgcaaactca atattgttgt tacaaaatat
    13561 ccatgaagca agtttatatt aaacaaacaa ctcgcataaa acaattgtta tccttaaatt
    13621 tcaacaaatt cttaataaat ttatctctat tttaattacg accaaattaa taggttttcc
    13681 atataaaaag atgcataaaa taaatattta aataaattca atttgtattc acttgttttt
    13741 gtcccccaaa tacaccagca acaagcatgc tagcaccaat tgttaaaacg ataaacatat
    13801 acagtcccat ttgtaatgac gttaagaaaa cacccaacac aatccctaac ctagctagtg
    13861 tttctgaaaa atgaatacct aatgcattaa ctgcactata tgttcctctt ttagctttag
    13921 gaataatttt aaagcgttgt tctgaaacta taggcgaata aataatttca cctacagtcg
    13981 caattatcat aaaaacaact aataagccaa actgattaag ataggttaga ccactatagc
    14041 caacaatata aatcagcaaa ccagtgatta aagctttttt aaaatctatt ttcaatacaa
    14101 ctttcgagat tgaatacgtg agtaaaatga cgacgaccgt attaatcatt agcaagattg
    14161 ctaacatctt agcacctgta atatcatatg aacctatact tattgtttca aactgatcct
    14221 ttagtctaat agcaatatat gaggagattg aaaattcacc catcatgatg atactgaacc
    14281 ccgaaatcaa taacatataa ttacggtctt tcaaaactaa tttataactg cgaaatatat
    14341 tcattatttg taatttttga taacgacttg catgcctctt gtcatcactt tgctttactt
    14401 gatttcggtc ttgaggtaac caaatatata aaataaagag tacaattaaa aatatacaag
    14461 ctgctattaa gaaaagtagt aacatactgt agccatacat caagccacct aacaatgccc
    14521 caatagctac cgataagttc gtcatccaat agctaatctt gtaaatataa tgttccacgt
    14581 cttcggtaat tgcatccata attaatgtgt ccataactgg aaattgtaat ccccaaacga
    14641 ttgtaaatat ggcatatgca acacaaaaac caataatttg ccacaattga tgtgacccaa
    14701 atacgcccat gaacacaagc attatcacca tcgtcgcttg ataaataagt actagcaact
    14761 ttttcggaaa aatctcaata aggtaaccag atataatgga caatggaaat tttagaacca
    14821 ctaaaccaac aagatatata ccgacaattg attgacttaa catatctgtt aaatatagtg
    14881 ctataaacgg tataaatgct gtcgtaataa ttagctgtaa aaaattgcta atcaatcgta
    14941 ctttcaaagt cgtacttaag cgtttcatta ataactacac ccctatccac atatatacac
    15001 actaccattt atataaaatc tattattaga ttatacgttg aaacaaagaa tttttagtta
    15061 tacaaagtta tttaacaaac aaatgcatcg attttacaat gcatcttgca tataatgcct
    15121 caaaaaaatt agcactatat gactatatat tattccaaat gaatgaaatt tatcaataat
    15181 tgcgtaagtg aacacatatg tgcgtaaatg ttaaatcatt gtcctaacat agccgtgact
    15241 atacttagat gtaatcataa aaaaactact gccatcttta caacagcagt agtgcttata
    15301 tgattcattt atataaaatt gaaagcgtta atcgctagcc cagcatcctt attctattta
    15361 gctgtataac ttttactagc aatacgacca cttgcaacaa taatatcttt atcttggaaa
    15421 ggttcatgat tgaagaaacg ctctaaaata tctttaactc catcttcaat acgtttttgt
    15481 gcttctaaag tcatacctga atagtgaacc gtcatagcat ttctaggcat tgttctccat
    15541 ggatgatcag caggtgcagg ttgtggatac caaacatcac cagcatatcc ttgtaaatgc
    15601 tcggatgcta acgcttcaac taacgcatcg cgatttacaa ttttaccacg tgcagtattc
    15661 actaaataac tgtgtttttt catacgactt aaaacatctt tatcaaataa gttatcagtt
    15721 tctggtgtta atggtgcatg aattgtaatc gcatcacttg ttgaaacaag ttcatcaaag
    15781 cttacaaatt tagacaattt atggtcttgt tgattgattg gatcatagtg ttgtaatgtt
    15841 acattaaatg gcgctaatct ttcagcaaca agttgtccaa ttcgaccaaa tccaaaaata
    15901 ccaattgttt tgtgttgtaa ttcatgcgca tgattaccta cttgagacaa gttccattca
    15961 ccttctactg attgacgatg accttcttca tagtttctaa gaagtattaa taaatccata
    16021 accgcatgtt ctgccacact aactgtatta cttcctgtaa cttcaacgac accaatattg
    16081 tgttcacttg ctgccgctaa atctacatgg tcagatccta cacctgctgt aattgctaat
    16141 ttcaagttcg gtgctttttc aatacgttca cgagtcatat atgcaggata aaatggcgca
    16201 ctaataatca catccatatc tggtaaatgt ttatctaagt cttcaccatt atctgctaat
    16261 ataatgaact catgtcctct ttcctctaaa aatgttttta atcctaatgc ttttttagta
    16321 ttaagtaatt gattttcttg accttctact gcttctggaa ataatgctac gattttcata
    16381 tccatctctc ctaaatgata aattttgact tccttaaagt acgtgttgca tgttgcttgt
    16441 tttaaaaata tgacaaaaaa aaacggcacc gtttgacata ccaccccctt gaattattgt
    16501 taaattcaaa gcgaagttag caaacagcac cgtgtacctt acaccagcaa cacttattct
    16561 attgacttat cgaaaacaaa tattgaaatg acttcatcat cattcaaatc aatatctgta
    16621 aataatttta caaacttagc gcctacaaga ctttccatct catttacatc tatttcttta
    16681 tacatctgtt taatcttctc tgtgcgtgta taatggagca tcgattcatt gcgtttgtcg
    16741 tttaggtaaa aactctcaac tttactcaaa acacctgtca tatgtgcaat cgcccaatta
    16801 tctttaaacg acactcgaat actattgggt cctttaccca cgtattcttt acgataagat
    16861 cttactagat tcgagaattg ttgttcttta gtatgcgtat ctatagtatt caccttcttt
    16921 aaaataagaa gttcattaat tgttcacatt ttcattatac accttattaa cactttttca
    16981 atgataacga ttattattat tacgaaatgt atattttcct agttaaaagg atataacaaa
    17041 ttaacagaaa atgtttataa actaagattt atattatttt aaaaaatgtt tcaacaaacg
    17101 catctaaaca aaaaatcagc ttatcacact taccccttca actctgcttc aagtttacct
    17161 aaatgtctta atgttggtgt caatgctgca aagaagaatc cttcttttaa cttgcgaact
    17221 tgtggcgaat atggtgtgta cgctcttgaa ccaccattga caacagaagc ttgatttaca
    17281 tctagcaata aatagccgat gtccttcttc aatgatatta attcatttaa atgtgaagtt
    17341 aagttaccgt catctaatat tgcataatat tcttctctaa gttgacgata acgtttttta
    17401 aaagcttcaa catcatactc taaatattga tttattccgt tttgcgcatt tgaaaatgca
    17461 tcaattaact ctaaagaact tttaattgag cctaatccta ttggaatttg ataagcaata
    17521 aattgtgggc gaatagttgc cgcaaactgc ttcgcatcat gcgtgataat ttgtgattgt
    17581 ggcactacga cttgattcaa agtaatttga tacgtagccg agccatttac ccctaaaaaa
    17641 tttgtttttt caacgagagt gataccatct tgattggcac gtagaatgaa catgacaaat
    17701 tcatctgatg attcatgttt cgaaatcgca ccaaaataat ggtcttcttg aatattactt
    17761 acagctggca tacgtccact gacaaccaat tgtccatcaa cataagtgtg ttcaaggttc
    17821 aacttttcta aatcattaaa tgacttcatc ggattagaca atccggtagc acctaatatt
    17881 tctccagata acaattgctg ttgtaagtca ttatttaaat gtggctgcgt ggcattttct
    17941 aaatacgttg aaaaagctaa ttggcaccat aaacaaaatc ctgttgtcaa acaagactgc
    18001 gatacagctt cgattacttc agcatttttt ctcaatgccg cctcaccgaa ataaccatct
    18061 acaaataatt gttgaataaa tgttctcgga taatacgtcc cttcatcaac ttctactaaa
    18121 tgaggatcta attgttcttt gataagcgtt tctaaagtca tcatcatctc tccttattca
    18181 ccaaatcttc taagtataaa ctgctcaata taactaatga atcgatcaat aataaaacca
    18241 aataatccga taaagaatat tgctgctaaa acatcttcta agttcaacat atttcgtgca
    18301 tcaacgatta aaaaacctaa tcccgattgt gcaccaatca tttcaccaga aactaaaaat
    18361 atccaacttg ttcctaccgc catatgtatc ccagccatga tttgtttaaa tgccccggga
    18421 aataatatat tgcgatacaa tgaccaccca gttaaattta aatttgccgc tatttttaaa
    18481 tattgaggtt caatgtctct aacgccttta atagtattga acacaattgg gaaaaaagcg
    18541 gcgataaaaa taatcgcaat cgctggcaaa ctaccaatac caaaccatag aacaacaaat
    18601 ggtgcccatg ctatcggaga tatcggtcta atcaattgaa atagcggttc gatagcgttg
    18661 tatagccaac gattccttcc aagcaagaag cccaatggaa tagcaaccaa caatgcgaca
    18721 acaaagcccg ctacaaatct ccataaacta attgctaaat gttggaaaat ttctccagta
    18781 acaatgaaag tccatatact ttttcctaca agagcaggac caggtaacaa tacaggttgg
    18841 taatgcccaa taataatgac catttcccaa atgcctaaga aaataataaa tgtgataata
    18901 ggtaatataa atttgttatt tgtgggacgt gtcatgaacg cgatgcctcc ttatacaatg
    18961 acggttcaac aaagtcatca tatgcaggtg gattaaacaa atgatgttgt tttaccaatg
    19021 tcgtaatttc ttgatagccg gatggcttaa ttgttaaatc accataggat gtccatgccg
    19081 ctgactgtgt taaaacgtca cgactttgtt taaaatgatg cgtcataatg tctacacttt
    19141 gcttgcgatc attcatttta aagccagact ttttataatc ttgtacaaat gcttgcgcta
    19201 catccttgtg ttgatcaagc aattcccctc tcagtactag cacacagcaa tacgcatcag
    19261 gtataacgtc atcaccatgt ttcaaagtct tacctttgcc taacttttca cccagtgcac
    19321 cgaatggttc ggctacagaa taccctgtaa ttctgtgttc actcaatgcg gctggcattt
    19381 ctgctggcga catttcatga tagctaaaat gccccggttt aatctttaat tgtttacgta
    19441 attcctcaag taaaagataa tgtgttgaat aacgatgtgg tataccaaaa tggtaatcat
    19501 cgccattatt attaaattca tttaagtgca tacctttttg tcccataatg acattgcctt
    19561 catgatggcc caatgccaca gcctttatat ttgagccctt ctgttttgat ttcatcgcta
    19621 gctctattaa agttgatgca ccatcaatac gaccactgtt taatgcgtcc attaaatctg
    19681 gccaattatt gaatttaact aattctagtt tatatttcgg atgattgtat tgtgataata
    19741 attttttagt catcatcaaa ttagctgaat gtgtaatcgg caaatatcca attttaatca
    19801 cttgctgatt ttgggcattt ttagacggtt ctttagacgt cctttgccaa tcacatcctg
    19861 taattataaa gattccaatg atgacgatta tgcttaacct tttcatcgtc actcactcct
    19921 tataaataat attcaggttc aacttgatga tgattcaatg caaatgtttc cataatttca
    19981 ttacgaacct taagtaggtg gctatcatta cgactgcgtg gatgtgatgc tgtaatttca
    20041 tattgagaaa taatattgca cccttcacct aacagaacaa tgcggtcgga aagataaata
    20101 gcttcatcaa tgtcatgcgt cactaaaata atagttgatt gcgttttatg ttttagttgc
    20161 actagttgat cctgaagttt ataacgtgta aatgcatcta atgcacctaa tggctcatcc
    20221 atcaatataa cgttaggctt atgcacatgc gctcgacata gtgccacacg ttgtttcata
    20281 cccccggaca gttgctcggg aaaatgcttt cccctgcctt ctaaatcaac taatttaagc
    20341 tgtgcgttaa tctcttcatc actaattttc tgtcgaaatc caatcctaat gttgtcatta
    20401 atcgttttcc atggcagcaa attatgatgt tgaaatagca ttaaacaatc tggagacggc
    20461 tgttgtttaa tttcgttatc aataatgaca cgtccagatg atggatgaat aaatccaccg
    20521 atgatattga gtaaagtaga ctttccgcaa ccactttttc ctatgaaagt gactatttct
    20581 cctttgctaa tgtccaaatt aaagttatga attactttat gtgatccaaa gtgatgttgt
    20641 aattgttgta ttttaatcat gacaagcccc cttttaatcc ctactatttt aataggaatt
    20701 aagtgtatat ctaaagggct atgccaaaca cagcccttat atcgtgaatt gttatatcta
    20761 tagatgatta tggtttggta tgagtgctct tataacataa gcgttattca attaacgacg
    20821 ttatattaat ttcagctcta ctaaatttca ataaaatgat tgttttagac aatacttgca
    20881 tttagttcat tagtttcgtc atattaacta cttacattta tagcattgag ctattctaat
    20941 taactgcaat agaaatccac gtcaaaccta tatacatata tctcatcaac ttgttgtctt
    21001 attaaagttt ttcgatgcca tcaatcggtg tatcaactgc tttgcgcgcg cgacgtaccg
    21061 cttcttcatc aggtgcgttg tataaacaaa tacatttaga catatcttca catacatatg
    21121 tgcgtttaaa ttcaacttct ggcacttctt cataatgaac agaatttttc tttttacgtg
    21181 ctaaatattg atccatcgta atgccttccg gaatgttcca agttacaagg taatcaacat
    21241 gtgcatcacc atttttaact tcatctaaat ctttaccaac taagcgtact tctttgacta
    21301 attgaatact aatatctgct tctgttaaaa attgtttagc ttctgcttcg tcatttgctt
    21361 ccacaataaa ataaccatga gttaaatttt cagtagcttg tacttcaatt aatgttggaa
    21421 ttgtcttcgt agatagtgat gctgcctttg cttctagttc ttctttcgtt gatgcaaaat
    21481 caagattgtt agcttctaat aaaaataatg tcattaataa gacctcctat aacaatggtt
    21541 gaaaaaatat tactataatt cttatcggat tacaaggata ttgagcgatt tttttaatat
    21601 ttagataaaa tcctgtaaaa tatattttca aagagtataa aaatcatgtt agggtataag
    21661 taaataaaca tatatatagg gggctatcat tatggataaa gaatctgtcg tagcaagttt
    21721 agctagaaac aaaaagattg ctgtagaaac aatggccggt caaagataca tcatagaacg
    21781 cattttacat acaaatgatg aaaaacatat tcatattttg aaacctaaag atgtcgtgtt
    21841 agatgtagat agcatcaaag aaattgacga aaatcattta aatgatgcaa cataatccaa
    21901 aacaaacgtg ggagtgggac agaaatgata ttttcgcaaa atttatttcg ttgtcccacc
    21961 ccaacttgca ttgcctgtag aatttctttt cgaaattctc tgtgttgggg cccacacccc
    22021 aacttgcatt gcctgtagaa tttcttttcg aaattctctg tgttggggcc cctgactaga
    22081 attgaaaaaa gcttgttaca agcgcatttt cgttcagtca actactgcca atataacttc
    22141 gtagagcata gaacattgat ttatgtccca gcctgtacaa tgttttattt atgttaattt
    22201 gtcgaagtat ggctcatcta atggattcaa ttgcaagttt tcaatttgca acgttgaatg
    22261 tgcaatgcca tatttttctt tcaacaatga tgatacttga tcaatcgctt gataatcatc
    22321 accctcatat tttttatcta acacaacatg ggcacttaat gaataatgct ctgttgtaat
    22381 actccacaaa tgaaattcat gtacatctaa tatgccatct atgtttttaa tatctgccat
    22441 aatttgatca gtatccaaat gttgaggcac actttccatt aaaattaacc acgcattacg
    22501 cgtaatttta taaccaccac gtaaaatgat gagtgaaatt acaatactaa tgattgggtc
    22561 gatgatgcgc catcctgtaa agtaaatcaa tacaactgca acgatgacac caatagagtt
    22621 caataagtct cccatgaaat gccataatgc actttgaata ttgatattgt cttcttgttt
    22681 taaagacctt acaaggataa ttgtcaaaat aatattaacg agtaaaccaa tactagcaat
    22741 cataaacata atgccacttt caattggttg cggataaata atacgtacaa tagcttcata
    22801 taaaatccag attgaaatta caattaatgc taaaccattt aaaaatgcag ctaatatctc
    22861 aaatcttaaa tatccaaatg tgtatcgtgc agtcggcttt ttacttgcaa aataaatggc
    22921 caacatagat aaaccaagtg ctaatacatc actaagcata tgaaatgaat ctgacagtaa
    22981 tgccaatgaa ttagatacta aacctccgac aaattcaatc actgtaaata acaatgtgat
    23041 gataagtgat gcccacagcg tctttttaga actttgttga attttttgat gattgacatg
    23101 atgaaaatat ttgtatctgt ttatcgtatt cgcctttttc ccattcatac gtttgccact
    23161 cctaaataaa tgcgtcgtat ttaatattgt cgcttttaaa aataattctt tatagcttct
    23221 caggttaata ttaatgcttc cgtaattaac catgatttat acatcatcgt catttacatg
    23281 acaactcatt aatctttacc ctttatagct ttatgtaata tcaccttgga aaacttttac
    23341 agcacactcg ctctttgctt tattcctatc atatagaaaa ttttaataat gtacgagatt
    23401 attgtgttag ttacataaaa atgacaaaaa agtatctcaa atttattgtt tagtttcatt
    23461 aagttcaaaa aatcgcaata actttaatga ttgttccgca tattaaaaca aacgatgact
    23521 ttgcatattg aacttcatat ttctaaatca ccataattaa taaacaactc tgtccttata
    23581 atagccgcat cgcaaattag cataattaaa aacagcagta agatgatttg caattaaaaa
    23641 ccatcctact gctattattt tagtcattat atcctaatat catgatacgt gttcctatat
    23701 attttgtttt gcttaaaatc taaacaacgc cgttaaatgt taagacttac cgctagactg
    23761 acctacaaac acgcttcaga aacgatattt attctagtac aaacctttta aagcattgct
    23821 tgtatcaata taaatatttt taacttgttg atagttactt aacgcacctt tataagtttc
    23881 tcgaccgata cctgattttt tataaccacc aaatggtgcg ccttctggta cttggttgta
    23941 agtgttaatc caaatacgtc ctgtacgtac agctttagca atatttaatg cacgtgtgat
    24001 attttgagaa aatacaccgc ctgctaaacc atactcagaa tcattagcta tatcaattgc
    24061 ttcttgatcg tccttcactt taatcactgt taacactggt ccaaatattt cttcttgtgc
    24121 taatttgtga tgattgtctg gcacagcaat taatgtcggc tcaaagaaga accctttatc
    24181 taatccattt tcagttaagc gatgaccacc tgctaaaatt tgtgcatctg attcttttgc
    24241 tgcatcaata tatgattgaa ttttatctaa ttgatcctta ccagtttgac tacccatttg
    24301 tgtagcttca tcttgtggat ctccaacttt aatatttgaa aatgcctctt gtaaacgtgg
    24361 caccaattga tcataaattt tttcatgaac taataatcga gaacctgcac tacatacttc
    24421 accttggttg aataaaatac ctaactgaat accttcaact gcaaggtcta aattagcatc
    24481 atctaatatg atattggcgc ttttaccacc aagctctaat gtagcgggta ctagatgttt
    24541 tgctgcagct tcggcaactt gataacctac atcagttgag cccgtaaatg ataatttatc
    24601 tacaccatca tgattgaaaa ttgcattacc tgattctgaa cctttacccg ttagtatatt
    24661 gacaacacct ttaggtaata cctcttggaa aattttagca acttccaata aacttaatgg
    24721 tgttgaagac gaaggttgaa tcacaattgt attacctgca gcaatggctg gcgcaatctt
    24781 ccatgcagct aatagcattg ggaagttcca agcaacaaca gcacctacga cgccaatcgg
    24841 ctcatgtcgt acgatactca ttgtgtcttt atcgatatca tttactgtac cttcttctgt
    24901 ttcaataaca cttgcgaaat aatggaaatg tcttgcagca aatggaatat caattgctgt
    24961 tgtctcacga atcggtttac cattatttaa tgtttcaatc attgcaattt tatctttttg
    25021 tgccattaat ttatcaccaa tatcacgcaa catttgtgca cgttctgatt tagaagttaa
    25081 tgaccatgat tcaaatgcat cttgcgccac tttgactgca tgatcgacat ctttatcttt
    25141 tgctcttgta atatgtgata gtgtttctcc agttgctgga ttagtcactt cgattgtttc
    25201 gtcactgcta cctttaacaa attccccatt gataaataaa ccataattct ctgctatata
    25261 atctcgaacg tttactgcca tattatctct cctttgttta ttggctataa tataaatctc
    25321 ttagcatcat atttaataat gcttttaaac gatatgattc acattatata gtaagcgttt
    25381 acattttata aaataatcag ctcacagaag tctcattttt aaaacttttg cgtcgttttt
    25441 gcacataaaa ataatcttat tttcgaaaac atattgcact tttcaaaaat aaaacacata
    25501 atataactat aatacttgag aataattctc attaaatagt ttggttgtta tgaaagtcgc
    25561 gcttaaaatg cgttaatgtg acaaggataa ttcaacatcg tttcatttta ctgagtcatt
    25621 gcacttatca tacacattat atttagcatg agttatatta ctaaaacaat attacatgcg
    25681 cattagtact agaatggagt tttttcaatg cgacttattt taatcgtcat tggcttaata
    25741 tttacagcgc ttggtattgc aggtgccgta ttacctttac tgccaacgac ccctttttta
    25801 ctcgtagcag ttttttgttt tgctcgaagt tcagatcgct tttacaattg gcttattaat
    25861 caaaaaattt ataaagaata tgtagaaaac ttttatttac atcgaggcta cacgctacaa
    25921 cagaaaatta aaattttaat tagcttatac attgtaatag gtttttcaat ttatatggtg
    25981 gatgttcttg cagtacgtat aggattaatc ataatggtta tcatacaaac cgctgtactc
    26041 tttacatttg taaaaacatt acccaaatca aatcataaaa tagaggagtg attgcccatg
    26101 tttatggcag aaaatagatt acaattacaa aaaggcagtg cggaagaaac gattgaacgt
    26161 ttttacaata gacaaggcat tgaaactatt gaaggcttcc aacaaatgtt tgtcactaaa
    26221 acattaaata ctgaggatac agacgaagtt aaaatcttaa ctatttggga atctgaagat
    26281 agctttaata attggttgaa ttccgatgta tttaaagaag ctcataaaaa tgtacgttta
    26341 aaaagtgatg acgatggaca gcaaagtcca atattatcaa ataaagtatt caaatatgat
    26401 attggctacc actatcaaaa ataaatatat taaaaacccc tagtcaccgc tggttgtcta
    26461 aaccaacttc atgattaggg gttttttatt atttgtgacg taaaggtacg aattcatccg
    26521 gcttgtctgt gcgcaatcca aaataatatt ctattgcttc acaaatacgt cgtgatgctt
    26581 gtccatcacc atatggatta ttcgcttgac tcatacgttg atatgcttca tcatcctcaa
    26641 tcaattgttt cacatttcga acaatattgt cataatctgt gccaattact ctcgatgttc
    26701 ccgcctcaac gccttcggga cgctctgtat gattccttaa taccaacaca ggttttccaa
    26761 atgtaggagc ctcctcttga ataccaccag aatctgtcag cacgaggtac gattgatttg
    26821 taaaattatg gaactcaatc gcatctaatg gctcaattaa ttcaatccga tttctcccag
    26881 ataaatattt ttcggcaatc gctcttacct ttggattacg atgcattgga taaatgaaga
    26941 caacatcttt atattcatct gccaaatctc ttactgcttt aaaaatctga tgcatcggtt
    27001 ccccaatatt ttcacgacga tgcgctgtta gtaaaataac tttcttgcct ttatgtttat
    27061 taataatcgt tgaaacaaaa tcattttgaa ctgttgttga caatgcgtca ataactgtat
    27121 ttccagtaat aaagatacgc tctttgtctt tgttttcaaa aagtaaattt ttagctgcaa
    27181 ttactgttgg cgcaaaattc aattcagcaa tattacttac catgactcga tttaactctt
    27241 caggaaatgg tgagtatttc tgatgtgttc gaagtccagc ttctacatgt ccgaccggaa
    27301 tttgatgata aaatgctgcc aaacttccta caaaagtcgt tgtagtatca ccatgtacta
    27361 aaatcatatc cggctgttcc tcattaatga tgctatcaag tttagcaagc gcattagccg
    27421 taaggtccgc taacgtttgt tgatcttgca taatatttaa atcatgatca gcttgaatat
    27481 caaatatact taacacacta tctaacatat ctctatgttg tgctgtaatc acaatgttcg
    27541 cttcaaagtt cccattatga tcaatttctt ttactaatgg tgccattttt attgcttcgg
    27601 gtctcgtacc gaaaataacc ataatttttt tcataacatt atcctctctg aagttcaaac
    27661 acatgacagc ttatctttcc ttaatcatgt atgccctagt ttgacacatt ttatttgtcg
    27721 ataaaattaa atatattgcc ataattataa tacgatacat cttcaaatga tgatttcaca
    27781 acattttttg tatcaaaaat cactttatgc ttcattttat caaaatgact gtccgataaa
    27841 tttttaaatt ctgagtggtc acttaaaatc aatactagcg atgcgtcttt gacagcatgt
    27901 gacatatcat gttccacaaa atctaattca acatgtggat cataagcaca tacttctatg
    27961 tctggttctt gatttaatag ctcataaata tcaaatgctg gcgattctct tatatcatca
    28021 acatcacctt tataagttaa accaaatact gtgactttat tcccgctcaa tgctttgatg
    28081 atttgcttcg ttgtatcaac aacataggcc ggcattgaat tattaatttc acgtccagtt
    28141 tgaattaact ttgcattttc agggtcttta gcaataataa agtacggatc aacagctaaa
    28201 caatgaccgc ctacacctgg accaggttga tggatattaa cacgcggatg tttgtttgcc
    28261 atttcaatca catctaatac attaatattt aagttgttgc aaatttttgt taattcatta
    28321 gctaaagcaa tgttcacgtc tctatatgtg ttttccatta gcttactcat ttcagcagta
    28381 cgtgcatctg tttcaatcat ttctccctga acaaatgtgc gatagacacg tttacccgct
    28441 tcaatacaag ctttagtcac accgccaatg atgcgattgt tatgaactaa ttcttctaaa
    28501 atttttcctg gcagtacacg ttctggacaa tgcactaaat aaatatcttc acctattgta
    28561 aaccctaaat tttcaatgac tggttttaca aaatcatcca tcgttttagg cgcaattgtc
    28621 gactctacaa taatagtatt cccttttttt aaaaatggta aaatactatc taatgcacgc
    28681 ataactagcg aaatgtcaca tgaccggtac tgatcatcat tattcggcgt cggaacggca
    28741 atgataaaaa catcagatgc ttctggcgtt gtagatacct tcaattttcc cgatgacagt
    28801 acctcttcat aaatctcttg taatccaggt tcttcaatac taatttgacc actttgtaac
    28861 ttatcaatcg tttgctgatt aatatcaaca ccaagcacat cgacgccatg ctttgcaaac
    28921 ataattgatg ttggtaaacc aatataacct aagccaacta ctgttaactt catactataa
    28981 cctccaaata aaacgatttg tatgttgatt ttatttaata gatgacttat gccttatctt
    29041 tggttgtcgt ttcatccatg atgtccgcaa taacaagtga catttttcca ggaataactt
    29101 caagtgcatt attattttca tataacgtat tgctgtatat taaattgccg aataattttc
    29161 taaagaccga caacttatta aaatacttat ttaacactga aggcatgttg atcaataccg
    29221 ttttacgatg tgattggcga cgtatttcat acattactga cgatgtatca aagtaaaagc
    29281 tatcttgagg atgatacacg cctgtcactt ctaatgatat taattgatca ataaatgctg
    29341 tcagatgttt aatatataat gcactgcgct gattgttaat attgggaatg attggcagtc
    29401 gctttgacaa ttgcattaac cgttggaaat ttcctgggca atgtgcacca taaatcattg
    29461 gtggtctcac aattgctact ttaaacgaat cactaatcaa ctcttgtaat gcttgttcag
    29521 cgaacttttt ggaaatacca tagttggtcg tagggttcat tggtgtttgt gtatcaattt
    29581 gatctgattt accaacctga ccttcttttc cataaactgc catagtactc ataaaaataa
    29641 attgtttaac gtcttcagat ttagcctttt gtgccaattg tttcgtcagc aacatattca
    29701 cttgcatata atcagatagc cttgcttgag gtgaattgtt gtgaaccaaa gctgctgtat
    29761 gaattaaaac atcataatct ttgaacgagg tcgacttcca taattgattc ctaacattaa
    29821 tttgatctac ttgatgtcct tgttcaataa gcttatcttt taaagcatta ccgatatatc
    29881 catgtacgcc tgtaattaaa atattttttc tcattagtga tgcacacctt ctgaagtaac
    29941 gatattttta attgttttat atatgatata catatcaagc atcatagatt gatgtgttaa
    30001 gtaataatga tcatacgcta ctttttgatc atcagtgata tcatctctcc ccatcacttg
    30061 agctagtcct gtcacacctg gtctaatcgt atgcacgttc acttttgtac gtttttcgat
    30121 taattcgtat tgattataaa gcgctggtct aggacctaca attgacattt ctccttttaa
    30181 aacattcaat aattgtggca attcatcaat agaggtctta cgaatgacct tccctgtctt
    30241 tgttatatac gatgttgaat ccattaaatc agttgcaaca ttaggtgtgt ctattttcat
    30301 tgatctaaac ttataaatat taaacaattc attattaatc gtcggtcttt tttgtttgaa
    30361 aatggctggt ccaggtgatt ccattttaat tagtaatgct gtaattaaca gaatcggact
    30421 taaaactact aaaccatata ttgaactcac tacatcgaat aatcgcttca taattatctc
    30481 ctcacttcaa cagattgtaa tacttcatag tattaatttc tctcgtatat ttatctttaa
    30541 acagttgatg tgcgcgctca cccatctcgt gcaattcctt actcgagtga gtgttgatga
    30601 agttataaat ggcatgtgca tcgccattat caatttggat acccgcatca tattgttcaa
    30661 catgttgaac gatatcagat tgcttatcca tgatgagtac caacgctttc ttagctgcaa
    30721 gatagccata attcttgctc ggcacgccta aaccgacgcc ttctttaatc agcgatgcaa
    30781 tacatacatc cgcaattttt aatacgtcag catagtctgt acctgttaaa aactcaaaca
    30841 ttttaacatt ttcaatacga tggtcttcta ttgccgtttt gacatctgca aatttcttac
    30901 catgaccaca aagtattgtt aacgtttgag gctgatcctt atttaatttt aaaaatgaga
    30961 taagtgtctc catatcctgt aactgcccca tattaccgct atacaataaa attttgtcgt
    31021 attgctcacg gtaagcttta aatgtgtcat tatagatacg attgtcttgt aattgacgca
    31081 tgtcatacca gttaggaatc acatggatat tgtcagcatt tttagaaatt tgatgattta
    31141 gtaagtagtt tttcatttcc gtaccaagga caatgacatt ttcagcattc ttgtagacat
    31201 gtctattaat gtaacgcatc agtttatcaa tcatgctacc tggacgagtt gcacctgtct
    31261 taatcgcatt atcaggtgct atatcataca ccacaaaaga atattttttc ttaagcagtc
    31321 tgtgtaaaac gtctggtatt aatggcaaga ttggtggatt agagtaaaca agaatctgat
    31381 catatttcaa cattttaggt atattaatca cgaattttga aaataaacta aagaaattga
    31441 tgatccttcc aaccttactt ttgttattaa acctcgaata cttgagacgt cgaatgcaaa
    31501 taccacgatg catctcggtt ttagaaacct gtttatgatt actatattca tatggccatc
    31561 cacacatgac atcgacatta atgtgattcg caattaaatc ttccgccaat tgagttggta
    31621 acgtcgcaga agatacatat tccggataaa aatactgaca taaaatcaaa atcttttttt
    31681 cactcatcta atcgacgtcc tttttattaa tgaatttaga ctaagaattt ttgaactgtt
    31741 tatttttagc aaaataatag agtgctatga aaaatatcat gtacgatacg attaaaacaa
    31801 ttgtattaat agatacaaag tttaaaaaag ttaaattaag catttttgat ataacaaatg
    31861 ttgcagtagt acctattaaa aataacgctt gtagtattaa ttctaactgt tgtttttgta
    31921 atacaatcac agaaagtgat accgttgata caatcagcct tataacaaat aatgggataa
    31981 caatttttat aactgtaatt gcgtcaaccc agctatgtcc taataatata ttgactaatt
    32041 cttcactgaa caaataaagt ggcacataaa gaattataga aactataacc agtatggaac
    32101 taaatttaag taataaattt cggaagttac catgttcaat ataatagtca ttggcttgtt
    32161 gcataaatat ttttgataac cctaatgaaa taattgtcac tggtatgcct agtactctta
    32221 tggccatacc ataaataccc acttctgtat tggtatacaa aatacctata aagaacacaa
    32281 caaccgagaa agacaaacta tttaataata ttgatggtgt tgaataaact aactgatttt
    32341 tattttctaa aaataatgct ttagtttctt ctttatctct cacaatatta aagtgctttt
    32401 taaacgtttt atatcctagt gtaatgcctg cgatatagga taatgaaaaa ccaataatta
    32461 atcctaaact tcctaatgct aaaagtccga aaatgatttg aagtaaagcc attgatccag
    32521 ctctaaacac attaatcaaa cttaacactt tatactttct ttccttattt agataatttg
    32581 taaaaatgtt tgttaaactt accaaaaaca acataataat aatgaaaata aaatctaata
    32641 taaagtttgg gtataaacat gcactaatcg caaatattgg tatcagtatt aataaaataa
    32701 gcaatgatat caaaaaactg atttgtgaaa gtatattagc actacggtca tttttggtat
    32761 tcacaatcaa caaatcgtat cttgcattta ttattggtat taaaatcatt gcgatatttg
    32821 aaaaaattgt aaactcacca aattctgtag gtgaatatag tcttgtaata attggggtag
    32881 tgatgattaa tatgacttga gcgatgccac tgcttaaaat cattaaaaac gaatcgccaa
    32941 taaatttatt taatctcata attaacctca gattttcttg atgattttcg ctggattgcc
    33001 acctataaca acgttacctt ctggaaaact ttttgttaca accgacccag cccctacaat
    33061 tgtatgttcc cccaattcta ctcctggtaa tataactgag ttcattccaa tccaactata
    33121 attccctatt ttgacatctt cacctggtgc atgtgacttc aaatttttaa tattatgatt
    33181 agctgtaata atacctacgt taggcgctat ataaacacct ctacctatat atataactgc
    33241 tgaaaaatta ttaaaatacg tcccgggcga ttgaaaaata tgaatatcat ttttatcaaa
    33301 aacaatgtta ttagggttat gcattctaac agttatatct gcaggaaatg gcaaacttgt
    33361 atttattcct aacaacttga acggaacagc tttccacgcc cataaccatc cagccacttt
    33421 attttcaaaa taatatccag ttaaatattc tttcttataa aataataata gtggtgtatt
    33481 cattaatcta aatattttcg atttcataaa tgtttttaca atcattctat ccctactttt
    33541 ttagtaaatt tttttattat aatgcctggg ataatagcta taacatatac aactaaaatc
    33601 atagaaatca atgtgattaa tggtgtcggg tgactaaaaa tactcgtaat aagtctcata
    33661 taaatatatg taccaataaa tatcacttct aacgaatgac tcttccgaat aatatactca
    33721 ctcagaaatg caaaaaagat atttgcaatt aaattcaaat agaagaataa gggtccaaaa
    33781 taaataattc catatccagc actcgaaatt agatgaccag tcaattgttt actaccatat
    33841 atcaattggt taaaaagttg gctagtaatc aactgctttt tatctaaaaa gaaattaaac
    33901 ccaaaaacag ctctggtatt atcaaataaa tattgtttga acgaaccatt atagtaattc
    33961 aaatagtcta tagaagctgc aatattgcta ggtccataaa aatatgactg caacgtgtca
    34021 actattttaa gattacttac acttgtacta tttaacgcct cgctatatga actataatta
    34081 aacacataca attctttata tagtgtcatc aatgctaata cgaaaatacc tacagatata
    34141 ataatgatat tgatacgtct tttatgctta gaaaacaaga ttgacaccac tgttaaaaca
    34201 gcaaccattg tataaagttg ataacttctt ctttcaccaa caattaaact aatatttaaa
    34261 attcctatag ctagcggtaa tacaacataa ataattttag gattctcttt atacttttta
    34321 tatgaagcat atgcgattat taagaataat aacgctaagg caagttgaaa tagcattaca
    34381 aaaagaacat ttaaaccact tgttgcttcg gttcctcttc ccattgcatc tgttttaata
    34441 attaaaaatg atatgttttt gcgcacttcg ggaaaagcta caaaaatccc acaaataaca
    34501 agaccaaaca caatgtaata aattgatgat ccagaaagtg taaagctatt tcttgatacc
    34561 acttcatgtt tgaataattt agatagtatc aatataaata gcgagcctat gataaactcc
    34621 acactcatat aaataaccgc ttgattaaaa taagcttgtc ctaatggtaa attatctata
    34681 gttgaatcct ggaaaaacac tatcatagca ggtataaata ctaatctaag aaaacctaaa
    34741 acatcgtata caattttcga aaatttaaaa ttcggattat tcgagaagct tataaagagc
    34801 tttcctactc cataacttat aggtgctaaa aataataaag gtttatcacc taacataagg
    34861 caaggaaaca ttaaaatggc aataacatat gaagtgacat tatatatctt attcattaaa
    34921 tatcctgcct atctcttgat agaactgttt attattttca attacatcgt ttctataagc
    34981 ttctctttta tgattaatgt ccattacatc caaattgtta taccaatcca tcaattcatc
    35041 accaatacct tttaaatttt ctggattaat actaaaaccc agtccgaatt tattagcttc
    35101 tgtagcggtg aatgtatcgt ctgttgtaat aataggtttg tttaaaaaca acgcataata
    35161 cattcttatg gataaagcag tatctaaagc aatattttga ttaccaaaca aattattaag
    35221 tatatcaatt tcatttaaat atttcggtgt ttctttcaag tcaaagccgc cagaaaatgt
    35281 aatattatta ataaaatttt ctcgagcaaa gacttctaat ttttccgacc ctgttccaaa
    35341 atattgcata tggaaccttg aatcatttgc cagttccttt atcaattttt gatttatctc
    35401 attaaatcta acattaccaa taaaacctat attaattggc catctttttt taaacccatc
    35461 ggttacatta ctttctctaa taatactcat gttataacta taaataattc tatatttttc
    35521 agatttaggt aaaaatttaa gaaaaccttc tgaagataat gttgtcaccc tgctagcatc
    35581 aacgattttt ttcattctat acttaataag ttt
//
LOCUS       DAMZKC010000005        34569 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.005, whole genome shotgun sequence.
ACCESSION   DAMZKC010000005 DAMZKC010000000
VERSION     DAMZKC010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 34569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34569
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.005"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            26..496
                     /gene="ssb"
                     /locus_tag="POB55_000168"
     CDS             26..496
                     /gene="ssb"
                     /locus_tag="POB55_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDI6499166.1"
                     /translation="MLNRTILVGRLTRDPELRTTQSGVNVASFTLAVNRTFTNAQGER
                     EADFINIIVFKKQAENVNKYLSKGSLAGVDGRLQTRNYENKEGQRVYVTEVVADSIQF
                     LEPKNTDDNQQDLYQQQAQQTRGQSQYSNNKPVKDNPFANANCPIEIDDNDLPF"
     gene            526..1419
                     /locus_tag="POB55_000169"
     CDS             526..1419
                     /locus_tag="POB55_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HDI6499167.1"
                     /translation="MTGWISIDRSIQNHWLFKEKRTFSKFEAWIYLLMEANHSKAKVP
                     IGNQIVTVERGQRLTSILTLSDLFNWSRFKVKTFLDLLESDGMLEVKTTSKYTLITIV
                     NYDFYQSEQGRNQHQNDIKPTSKQHQSNINPTSKQHQTNTNNNDNKDNNEKNVNNEKK
                     KAAAFDFFQDNGFGFITPYNLDDLNYYLDSFENDSDEIVTASLKIAKDRNKVTWGYAK
                     SILNTWLNANLKSIEQVRAFEKQQIESKKQTYKPHVKQSKEKTPKWLTDGTRETKTAE
                     VDENLEKDREAFIKRLNSKWE"
     gene            1426..1644
                     /locus_tag="POB55_000170"
     CDS             1426..1644
                     /locus_tag="POB55_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499168.1"
                     /translation="MDAFDKYYLFDHDGNKMFSVTPHFKDGRHLVVGIKETKSNGRRW
                     YLDDYELNTLIDNEQMELGHQTNLFEYI"
     gene            1653..2057
                     /locus_tag="POB55_000171"
     CDS             1653..2057
                     /locus_tag="POB55_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000401967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HDI6499169.1"
                     /translation="MEIEIKFNEVFNAPMGSPRPRFSTKGRYVHTYMPTNYTKHKKYL
                     QSQMPRLNLENALKIELAFYFTPPESWSKKKKAQAIGQLKVTKPDIDNLIKTVLDACN
                     GHVWKDDNQITEITSSKRYGIEPKIIIRIEEI"
     gene            2070..2438
                     /locus_tag="POB55_000172"
     CDS             2070..2438
                     /locus_tag="POB55_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499170.1"
                     /translation="MARKARIVTINDKPYRFSKFEMELIESHGITAGMVSKRVKDGWE
                     LHEAMDAPEGTRLSEYREKKTIERLEQARLERKLERKRKREAELRRKKPHLFNVPQKH
                     PRGRYACYLMENDIFVKVKK"
     gene            2442..2684
                     /locus_tag="POB55_000173"
     CDS             2442..2684
                     /locus_tag="POB55_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499171.1"
                     /translation="MTDNARKEYLNQFFGSKRYLYQDNEGVAHIHVVNGTYYFHGHIV
                     PGWQGVKKTFDTAEELETYIKQHGLEYEEQKQLTLF"
     gene            2699..2947
                     /locus_tag="POB55_000174"
     CDS             2699..2947
                     /locus_tag="POB55_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1024 family protein"
                     /protein_id="HDI6499172.1"
                     /translation="MNNREQIEQSVISASAYNGNDTEGLLKEIEDVYKKAQAFDEILK
                     GLPNAMQDALKEDIGLDEAVGIMTGQVVYKYEEEQEND"
     gene            2940..3476
                     /locus_tag="POB55_000175"
     CDS             2940..3476
                     /locus_tag="POB55_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HDI6499173.1"
                     /translation="MTNTLTIDQLQELLQIQKEFDDRIPTLNLRDSKIAYVVEFFEWF
                     NTLETFKNWKKKPGKPLDVQLDELADMLAFGLSIANQVGVSSEEIKEAIESSFKNKEF
                     HNMFNFKDKEFAQDAVVSTPQIIFKEFYPDQLAIVIAIDIAYNLYSINQLIDAYKKKM
                     KRNHERQDGTADAGKGYV"
     gene            3513..3719
                     /locus_tag="POB55_000176"
     CDS             3513..3719
                     /locus_tag="POB55_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1381 domain-containing protein"
                     /protein_id="HDI6499174.1"
                     /translation="MTQYLVTTFKDSTGRKHTHITKAKSNQSYTVVEAESKEEAKEKY
                     EAQVKRDAVIKLGQLFENIRECGK"
     gene            3716..3919
                     /locus_tag="POB55_000177"
     CDS             3716..3919
                     /locus_tag="POB55_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499175.1"
                     /translation="MNQLRILLHDGSSLILHEDELFNEIVFVLDDFRNDDDYLTIEKD
                     YGRELVLNKGYIVGINVEEADDD"
     gene            3912..4148
                     /locus_tag="POB55_000178"
     CDS             3912..4148
                     /locus_tag="POB55_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499176.1"
                     /translation="MINIPKMKFPKKYTEIIKKYKNKAPEEKAKIEDDFIKEIKDKDS
                     EFYSPTMANMNEYELRAMLRMMPSLIDTGDDNDD"
     gene            4141..4527
                     /locus_tag="POB55_000179"
     CDS             4141..4527
                     /locus_tag="POB55_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499177.1"
                     /translation="MIKKLKNMDGFDIFIVGILSLFGIFALLLVITLPIYTVASYQHK
                     ELHQGTITDKYNKRQDKEDKFYIVLDNKQVIENSDLLFKKKFDSADIQARLKVGDKVE
                     VKTIGYRIHFLNLYPVLYEVKKVDKQ"
     gene            4524..4676
                     /locus_tag="POB55_000180"
     CDS             4524..4676
                     /locus_tag="POB55_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDI6499178.1"
                     /translation="MIKQILRLLFLLAMYELGKYVTEQVYIMMTANDDVEAPSDYEKI
                     RAEVSW"
     gene            4744..4944
                     /locus_tag="POB55_000181"
     CDS             4744..4944
                     /locus_tag="POB55_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1514 family protein"
                     /protein_id="HDI6499179.1"
                     /translation="MWIVISIVLAIFLLILLSSISHKMKTIEALEYMNSYLFKQLVKN
                     NGVEGLEDYENEVERIRKRFKS"
     gene            5232..7679
                     /locus_tag="POB55_000182"
     CDS             5232..7679
                     /locus_tag="POB55_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence-associated E family protein"
                     /protein_id="HDI6499180.1"
                     /translation="MLDKVTQIETIKYDRDVSYSYAASRLSTYWTNHNMAWSDFMQKL
                     AQTVRTKEDLTEYNKMSKSEQADIKDVGGFVGGYLKEGKRRAGQVMNRSMLTLDIDYA
                     AQDMTDILSMFYDFAYCLYSTHKHREISPRLRLVIPLKRNVNADEYEAIGRKVADIVG
                     MDYFDDTTYQPHRLMYWPSTSNDAEFFFTYEDLPLLDPDKILNEYVDWTDTLEWPTSS
                     KEESKTKRLADKQGDPEEKPGIVGAFCRAYTIEEAISTFIPDLYEKHSTNRYTYHEGS
                     TAGGLVLYENNKFAYSYHNTDPVSGMLVNSFDLVRIHLYGAQDEDAKTDTPVNRLPSY
                     KAMQQRAQNDEVVKKQLINDKMSDAMQDFDEIVNSDDAWSETLEITSKGTFKASIPNI
                     EIILRNDPNLKGKIAFNEFTKQIECLGKVPWNTNFKTRQWQDGDDSSLRSYIEKIYDI
                     HHSGKTKDAIISVAMQNAYHPVRDYLNKISWDGHKRLEKLFIKYLGVEDTEVNRTTTK
                     KALTAGIARVMEPGCKFDYMLTLYGPQGVGKSALLKKLGGAWFSDSLVSVTGKEAYEA
                     LQGVWLMEMAELAATRKAEVEAIKHFISKQVDRFRVAYGHYIEDFPRQCIFIGTTNKV
                     DFLRDETGGRRFWPMTVNPERVEVNWSKLTKEEIDQIWAEAKYYYEQGEELFLNPELE
                     EEMRSIQSKHTEESPYTGIIDEYLNTPIPSNWDDLTIFERRRFYQGDVDMLPTGNVDY
                     VERNKVCALEVFVECFGKDKGDSRGSMEIRKISNILRQLDNWSVYDGNKSGKIRFGKD
                     YGVQIAYVRDESLEDLI"
     gene            complement(7788..7886)
                     /locus_tag="POB55_000183"
     CDS             complement(7788..7886)
                     /locus_tag="POB55_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499181.1"
                     /translation="MNLMQHWKQTGLNLTARRERGVVSFVASMLHH"
     gene            8020..8310
                     /locus_tag="POB55_000184"
     CDS             8020..8310
                     /locus_tag="POB55_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HDI6499182.1"
                     /translation="MKESTLEKYLVKEISKLNGLCLKWVAPGTRGVPDRIIIMPEGKT
                     YFVEMKQEKGKLHPLQKYVHRQFENRDHKVYVLWNKEQVNTFIRMVGGTFGD"
     gene            8291..9658
                     /locus_tag="POB55_000185"
     CDS             8291..9658
                     /locus_tag="POB55_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDI6499183.1"
                     /translation="MEHLAIDFKPHSYQKYAIDKVIDNEKYGLFLDMGLGKTVSTLTA
                     FSELQLLDTKKMLVIAPKQVAKDTWVDEVDKWNHLNHLKVSLVLGTPKERNDALNTEA
                     DIYVTNKENTKWLCDQYKKEWPFDMVVIDELSTFKSPKSQRFKSIKKKLPLINRFIGL
                     TGTPSPNSLQDLWAQVYLIDRGERLESSFSRYRERYFKPTHQVSEHIFKWELRDGSEE
                     KIYKQIEDICLSMKAKDYLDMPDRVDTKQTVVLSEKERKVYEELEKNYILESEEEGTV
                     VAQNGASLSQKLLQLSNGAVYTDEEDVRLIHDKKLDKLEEIIEESQGQPILLFYNFKH
                     DKERILQRFKEATTLEDSNYKERWNSGDIKLLIAHPASAGHGLNLQQGGHIIVWFGLT
                     WSLELYQQANARLYRQGQNHTTIIHHIMTDNTIDQRVYKALQNKELTQEELMKAIKAR
                     IAKHK"
     gene            9671..10108
                     /locus_tag="POB55_000186"
     CDS             9671..10108
                     /locus_tag="POB55_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDI6499184.1"
                     /translation="MGNTIYDIKPGTFKYIESEIYNLNENKKEIKRLRLEILNPTKEQ
                     DSNIVYGPLQKGEPVRTTELMATRLLTNKMLRNLEEMVEAVESEYLKLPEDHKKVIRL
                     KYWNKEKKLKMEQIGHECHMHRNTVTTIRKNFVKAVAYHAGIK"
     gene            10265..10579
                     /locus_tag="POB55_000187"
     CDS             10265..10579
                     /locus_tag="POB55_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDI6499185.1"
                     /translation="MTKHNNIYKHGRKSYQYDWFYHSKAWKKLREIALDRDNHLCQMC
                     LREDIVTDANIVHHIIYVDEDFNKALELDNLMSVCYGCHNKIHANGNDKSNLKKIRVL
                     KI"
     gene            10706..11011
                     /locus_tag="POB55_000188"
     CDS             10706..11011
                     /locus_tag="POB55_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HDI6499186.1"
                     /translation="MKLTKKQLKEYIEDYKKSDDILINLYIETYEFYCRLRDELKNSD
                     LMIEHTNKAGASNIVKNPLSIELTKTVQTLNNLLKSMGLTAAQRKKIVQEEGGFGDY"
     gene            11001..12692
                     /locus_tag="POB55_000189"
     CDS             11001..12692
                     /locus_tag="POB55_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDI6499187.1"
                     /translation="MTIKVLNEPSPKLLTTWYAEQVTQGKIKTSKYVKKECERHLRYL
                     ENGGKWVFDEELAHRPIRFIEKFCKPSKGSKRQLVLQPWQHFIIGSLFGWVHKETKLR
                     RFKEALIFMGRKNGKTTTISGVANYAVSQDGENGAEIHLLANVMKQARILFDESKAMI
                     KASPKLDKNFRTLRDEIHYDATISKIMPQASDSDKLDGLNTHMGIFDEIHEFKDYKLI
                     SVIKNSRAARLQPLLIYITTAGYQLDGPLVDMVEAGRDTLDQIIEDERTFYYLASLDD
                     DDDINDSSNWIKANPNLGVSINLDEMKEEWEKAKRTPAERGDFITKRFNIFANNDEMS
                     FIDYPTLQKNNEIVSLEELEGRPCTIGYDLSETEDFTAACATFALDNGKVAVLSHSWI
                     PKHKVEYSNEKIPYREWEEDGLLTVQDKPYIDYQDVLNWIIKMNEHYVVEKITYDRAN
                     AFKLNQELKNYGFETEETRQGALTLSPALKDLKEMFLDGKIIFNNNPLMKWYINNVQL
                     KLDRNGNWLPSKQSRYRKIDGFAAFLNTYTDIMNKVVSDKGEGNIEFISIKDIMR"
     gene            12697..13935
                     /locus_tag="POB55_000190"
     CDS             12697..13935
                     /locus_tag="POB55_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDI6499188.1"
                     /translation="MNVIAKENIVTRIKKKLIDNWIDQSASKLYDFSPWKNKSFWGVI
                     NNTLETNETIFSAITKLSNSMASLPLKMYEDYKVVNTEVSDLLTVSPNNSLSSFDFIN
                     QIETIRNEKGNAYVLIERDIYHQPSKLFLLNPDVVEMLIENQSRELYYSIHAATGNKL
                     IVHNMDMLHFKHIVASNMVQGISPIDVLKNTTDFDNAVRTFNLTEMQKPDSFMLKYGS
                     NVGKEKRQQVLEDFKQYYEENGGILFQEPGVEIEPLPKKYVSEDIVASENLTRERVAN
                     VFQLPSVFLNARSNTNFAKNEELNRFYLQHTLLPIVKQYEEEFNRKLLTKTDREKNRY
                     FKFNVKSYLRADSATQAEVYFKAVRSGYYTINDIREWEDLPPVEGGDKPLISGDLYPI
                     DTPLELRKSLKGGDKNVNES"
     gene            13919..14692
                     /locus_tag="POB55_000191"
     CDS             13919..14692
                     /locus_tag="POB55_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HDI6499189.1"
                     /translation="MSMKAKYFQMKRKSKSKGEIFIYGDIVSDKWFESDVTATDFKNK
                     LDELGDISEIDVHINSSGGSVFEGHAIYNMLKMHPAKINIYVDALAASIASVIAMSGD
                     TIFMHKNSFLMIHNSWVMTVGNAEELRKTADLLEKTDAVSNSAYLDKAKDLDQEHLKQ
                     MLDAETWLTAEEALSFGLIDEILGANEIAASISKEQYKRFENVPEDLKKDVDKITKID
                     DVDTSELVETPKESMSLEEKEKREKIKRECEILKMTMNY"
     gene            14704..15867
                     /locus_tag="POB55_000192"
     CDS             14704..15867
                     /locus_tag="POB55_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDI6499190.1"
                     /translation="MPTLYELKQSLGMIGQQLKNKNDELSQKATDPNIDMEDIKQLET
                     EKAGLQQRFNIVERQVQDIEEKEKAKVKDKGEAYQSLSDNEKMVKAKAEFYRHAILPN
                     EFEKPSMEAQRLLHALPTGNDSGGDKLLPKTLSKEIVSEPFAKNQLREKARLTNIKGL
                     EIPRVSYTLDDDDFITDVETAKELKAKGDTVKFTTNKFKVFAAISDTVIHGSDVDLVN
                     WVENALQSGLAAKERKDALAVSPKSGLEHMSFYNGSVKEVEGADMYDAIINALADLHE
                     DYRDNATIYMRYADYVKIISVLSNGTTNFFDTPAEKVFGKPVVFTDAAVKPIVGDFNY
                     FGINYDGTTYDTDKDVKKGEYLFVLTAWYDQQRTLDSAFRIAKAKENTGPLPS"
     gene            15936..16214
                     /locus_tag="POB55_000193"
     CDS             15936..16214
                     /locus_tag="POB55_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HDI6499191.1"
                     /translation="MSLEEIKLWLRIDYNFENDLIEGLIQSAKSELLLSGVPDYDKDD
                     LEYPLFCTAIKYIIARDYESRGYSNDQSRSKVFNEKGLQKMILKLKKW"
     gene            16226..16558
                     /locus_tag="POB55_000194"
     CDS             16226..16558
                     /locus_tag="POB55_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499192.1"
                     /translation="MEFNEFKDRAYFFQYINKGPYPDEEEKMKLYSCFCKIYNPSMKD
                     REILKATESKSGLTIIVRSSKTEYLPQTNHLVKIDSALYSDKLFNIVEIRIDTPDIGY
                     NTVVLSEK"
     gene            16555..16956
                     /locus_tag="POB55_000195"
     CDS             16555..16956
                     /locus_tag="POB55_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499193.1"
                     /translation="MSVEIKGIPEVLNKLESVYGKQAMQAKSDKALNEASEFFIKALK
                     KEFESFKDTGASIEEMTKSKPYTKVGSQERAVLIEWVGPMNRKNIIHLNEHGYTRDGK
                     KYTPRGFGVIAKTLAASERKYREIIKKELAR"
     gene            16957..17352
                     /locus_tag="POB55_000196"
     CDS             16957..17352
                     /locus_tag="POB55_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HDI6499194.1"
                     /translation="MNILNTIKGILLSDAELKTHINSRIYYYKVTENAETSKPFVVIT
                     PVYDLPSDFMSDKYLSEEYLIQIDVESSNNQKTIDITKRIRYLLYQQNLIQASSQLDA
                     YFEETKRYVMSRRYQGIPKNIYYKNQRIE"
     gene            17387..18028
                     /locus_tag="POB55_000197"
     CDS             17387..18028
                     /locus_tag="POB55_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDI6499195.1"
                     /translation="MAEGQGSYKVGFKRLYVGVFNPEATKVVKRMTWEDEKGGTVDLN
                     ITGLAPDLVDMFASNKRVWMKKQGTNEVKSDMSIFNIPSDDLNTVIGRTKDKNGTSWV
                     GENTRAPYVTVIGESEDGLTGQPVYVALLKGTFSLDSIEFKTRGEKAEAPEPTKLTGD
                     WMNRKVDVDGTSQGIVYGYHEGKEGEAEFFKKVFVGYTDSEDHSEDSAGSLPS"
     gene            18120..18575
                     /locus_tag="POB55_000198"
     CDS             18120..18575
                     /locus_tag="POB55_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HDI6499196.1"
                     /translation="MTKTLKVYKGDDVVASEQGEGKVSVTLSNLEADTTYPKGTYQVA
                     WEENGKESSKVDVPQFKTNPILVSGVSFTPETKSIMVNTDDNVEPNIAPSTATNKILK
                     YTSEHPEFVTVDENTGAIHGVAEGTSVITAMSTDGSDKSGQISVTVTNG"
     gene            18633..18983
                     /locus_tag="POB55_000199"
     CDS             18633..18983
                     /locus_tag="POB55_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499197.1"
                     /translation="MIKFEIKDRKTGKTESYTKEDVTMGEAEKCYEYLELVNQENKKE
                     APNATKMRQKERQLLVDLFKDEGLTEEDVLNKMSTKTYTKALQDIFREINGEDEEDSE
                     TEPEEMGKTEEQSQ"
     gene            19025..19183
                     /locus_tag="POB55_000200"
     CDS             19025..19183
                     /locus_tag="POB55_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499198.1"
                     /translation="MEQYGWTLTEVRKQPYVKLLEILNEENKEETEEKQSEQKVITGT
                     DLRKLFGS"
     gene            19197..25397
                     /locus_tag="POB55_000201"
     CDS             19197..25397
                     /locus_tag="POB55_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDI6499199.1"
                     /translation="MNEKVEGMTLELKLDHLGVQEGMKGLKRQLGVVNSEMKANLSAF
                     DKSEKSMEKYQARIKGLNDRLKVQKKMYSQVEDELKQVNANYQKAKSSVKDVEKAYLK
                     LVEANKKEKLALDKSKEALKSSNTELKKAENQYKRTNQRKQDAYQKLKQLRDAEQKLK
                     NSNQATTAQLKRASDAVQKQSAKHKALVEQYKQEGNQVQKLKVQNDNLSKSNDKIESS
                     YAKTNTKLKQTEKEFNDLNNTIKNHSANVAKAETAVNKEKAALNNLERSIDKASSEMK
                     TFNKEQMIAQSHFGKLASQADVMSKKFSSIGDKMTSLGRTMTMGVSTPITLGLGAALK
                     TSADFEGQMSRVGAIAQASSKDLKSMSNQAVDLGAKTSKSANEVAKGMEELAALGFNA
                     KQTMEAMPGVISAAEASGAEMATTATVMASAINSFGLKASDANHVADLLARSANDSAA
                     DIQYMGDALKYAGTPAKALGVSIEDTSAAIEVLSNSGLEGSQAGTALRASFIRLANPS
                     KNTAKEMKKLGIHLSDAKGQFVGMGELIRQFQDNMKGMTREQKLATVATIVGTEAASG
                     FLALIEAGPDKINSYSKSLKNSNGESKKAADLMKDNLKGALEQLGGAFESLAIEVGKD
                     LTPMIRAGAEGLTKLVDGFTHLPGWVRKASVGLALFGAAIGPAVLAGGLLIRTVGSAA
                     KGYASLNRRIAENTILSNTNSKAMKSLGLQTLFLGSTTGKTSKGFKGLAGAMMFNLKP
                     INVLKNSAKLAILPFKLLKNGLGLAAKSLFAVSGGARFAGVALRFLTGPIGATITAIT
                     IAYKVFKTAYDRVEWFRNGINGLGETIKFFGGKIIGGAVRKLGEFKNYLGSIGKSFKE
                     KFSKDMKDGYKSLSDDDLLKVGVNKFKGFMQTMGTASKKASDTVKVLGKGVSKETEKA
                     LEKYVHYSEENSRIMEKVRLNSGQISEDKAKKLLKIETDLSNNLIAEIEKRNKKELEK
                     TQELIDKYSAFDEQEKQNILTRTKEKNDLRIKKEQELNQKIKELKEKALSDGQISENE
                     RKEIEKLENQRRDITVKELSKTEKEQERILVRMQRNRNAYSIDEASKAIKEAEKARKA
                     RKKEVDKQYEDDVIAIKNNVNLSKSEKDKLLAIADQRHKDEVRKAKSKKDAVVDVVKK
                     QNKDIDKEMDLSSGRVYKNTEKWWNGLKSWWSNFREDQKKKSDKYAKEQEETARRNRE
                     NIKKWFGNAWDGVKSKTGEAFSKMGRNANHFGGEMKKMWSGIKGIPSKLSSGWSSAKS
                     SVGYHTKAIANSTGKWFGKAWQSVKSTTGSIYNQTKQKYSDASDKAWAHSKSIWRGTS
                     KWFSNAYKSAKGWLTDMANKSRAKWDNISSTAWSNAKSVWKGTSKWFSNSYKSLKDWT
                     GDMYSRAHDRFDAISSSAWSNAKSVFNGFRKWLSKTYDWIRDIGKDMGRAAADLGKNV
                     ANKAIGGLNSMIGGINKISKAITDKNLIKPIPTLSTGTLAGKGVATDNSGALTQPTFA
                     VLNDRGSGNAPGGGVQEVIHRADGTFHAPQGRDVVVPLGVGDSVINANDTLKLQRMGV
                     LPKFHGGTKKKDWLDQLKGNIGKKAGEFGATAKNTAHNIKKGAEEMVEAAGDKIKDGA
                     SWLGDKIGDVWDYVQHPGKLVNKVMSGLNINFGGGANATVKIAKGAYSLLKKKLVDKV
                     KSWFEDFGGGGDGSYLFEYPIWQRFGRYTGGLNFNGGRHYGIDFGMPSGTNVYAVKGG
                     IADKVWTDYGGGNSIQIKTGANEWNWYMHLSKQLARQGQRIKAGQLIGKSGATGNFVR
                     GAHLHFQLMQGSHPGNDTAKDPEKWLKSLKGSGVRSGSGVNKAASAWAGDIRRAAKRM
                     GVNVTSGDVGNIISLIQHESGGNAGITQSSALRDINVLQGNPAKGLLQYIPQTFRHYA
                     VRGHNNIYSGYDQLLAFFNNRYWRSQFNPRGGWSPSGPRRYANGGLITKHQLAEVGEG
                     DKQEMVIPLTRRKRAIQLTEQVMRIIGMDGKPNNITVNNDTSTVEKLLKQIVMLSDKG
                     NKLTDALIQTVSSQDNNLGSNDAIRGLEKILSKQSGHRANANNYMGGLTN"
     gene            25397..26221
                     /locus_tag="POB55_000202"
     CDS             25397..26221
                     /locus_tag="POB55_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HDI6499200.1"
                     /translation="MQSFVKIIDGYKEEVITDFNQLIFLDARAESPNTNDNSVTINGV
                     DGILPGAISFAPFSLVLRFGYDGIDVIDLNLFEHWFRSVFNRRHPYYVITSQMPGVKY
                     AVNTANVTSNLKDGSSTEIEVSLNVYKGYSESVNWTDSEFLFDSNWMFENGIPLDFTP
                     KYTHTSNQFTIWNGSTDTINPRFKHDLKILINLNASGGFELVNYTTGDIFKYNKSIDK
                     NTDFVLDGVYAYRDINRVGIDTNRGIITLAPGKNEFKIKGDVSDIKTTFKFPFIYR"
     gene            26230..27813
                     /locus_tag="POB55_000203"
     CDS             26230..27813
                     /locus_tag="POB55_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDI6499201.1"
                     /translation="MDYHDHLSVMDFNELICENLLDVDYGSFKEYYELNEARYITFTV
                     YRTTHNSFVFDLLICENFIIYHGEKYTIKQTAPKVEGDKVFIEVTAYHIMYEFQNHSV
                     ESNKLDDDSSETGKTPEYSLDEYLRYGFANQKTSVKMTYKIIGDFKRKVPIDELGNKN
                     GLEYCKEAVDLFGCIIYPNDTEIGFYSPETFYQRSEKVIRYQYNTDTVSATVSTLELR
                     TAIKVFGKKYTAEEKKNYNPIRTTDIKYSNGFIKEGTYRTETIGSKATINFNCKYGNE
                     TVRFTIKKGSQGGIYKLILDGKQIKQISCFAKSVQSETIDLIKNIDKGKHVLEMIFLG
                     EDPKNRIDISSNKKAKPCMYVGTEKSTVLNLIADNSGRNQYKAIVDYVADSAKQFGIR
                     YANTQTNEDIETQDKLLEFAKKQINDTPKTELDVNYIGYEKIEPRDSVFFVHELMGYN
                     TELKVVKLDRSHPFVNAIDEVSFSNEIKDMVQIQQALNRRVIAQDNRYNYQANRINHL
                     YTSTLNSPFETMDIGSVLI"
     gene            27813..28103
                     /locus_tag="POB55_000204"
     CDS             27813..28103
                     /locus_tag="POB55_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499202.1"
                     /translation="MATEEVKIKALLENDKQYFPATHWKAINGIPYAGSSDIDGLPQD
                     GIISVDDKNKLDKLKIGEAGIIQNSIVQKSPNGKLWKITVDDSGKLGTVLFY"
     gene            28119..30029
                     /locus_tag="POB55_000205"
     CDS             28119..30029
                     /locus_tag="POB55_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499203.1"
                     /translation="MENLYLIKDLGALAGRDYRAKEIQNLQRIEQFALGLTTEFKLHQ
                     KAKTMQHFAEQIYYNGRSQAAVNKSLQSQINALVVAPRNNSANEIVQARVNVNGETFD
                     TLKEHLDDWETKTQINKEETIRELNKTKQEILDIEYRFEPDKQEFLFVTELAPLTNAV
                     MQSFWFDNRTGIVYMTQARNNGYMLSRLRPNGQFIDSSLIVGGGHGTHNGYRYIDDEL
                     WIYSFILNGNNENTLVRFKYTPNVEISYGKYGMQDVFTGHPEKPYITPVINEKENKIL
                     YRIERPRSQWELENSMNYIEIRSLDDVDKNIDKVLHKISIPMRLTNETQPMQGVTFDE
                     KYLYWYTGDSNPNNRNYLTAFDLETGEEAYQVNADYGGTLDSFPGEFAEAEGLQIYYD
                     KDSGKKALMLGVTVGGDGNRTHRIFMIGQRGILEILHSRGVPFIMSDTGGRVKPLPMR
                     PDKLKNLGMLTEPGLYYLYTDHTVQIDDFPLPREWRDAGWFLEVKPPQTGGDVIQILT
                     RNSYARNMMTFERVLSGRTGDISDWNYVPKNSGKWERVPSFITKMSDINIVGMSFYLT
                     TDDTKRFTDFPTERKGVAGWNLYVEASNTGGFVHRLVRNSVTASAEILLKNYDSKTSS
                     GPWTLHEGRIIS"
     gene            30029..31495
                     /locus_tag="POB55_000206"
     CDS             30029..31495
                     /locus_tag="POB55_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HDI6499204.1"
                     /translation="MSNLEKSVAINLENTAHYENISNLDITFRTGESDSSVLLFNIIK
                     NNQPLLLSEENIKARIAIRGKGVMVVAPLEILDPFKGILKFQLPNDVIKRDGSYQAQV
                     SVAELGNSDVVVVERTITFNVEKSLFSKIPSETKLHYIVEFQELEKTIMDRAKAMDEA
                     IKNGEDYASLIEKAKEKGLSDIQIAKSSSIDELKQLANSRISDLENKAQAYSRTFDEQ
                     KRYMDEKHEAFKQSVNSGGLVTSGSTSNWQKAKITKDDGKIMQITGFDFNNPEQRIGD
                     STQFIYVSQAINYPRDVSTNGTVEYLVVTSDYKRMTYRPNGTNKVFVKRKEAGSWSEW
                     SELAINDYNTPFETVQSAQSKANMAESNAKLYADDKFNKRYSVIFDGTANGVGSTLYL
                     NESLDQFILLIFYGTFPGGDFTEFGSPFGGGKISLNPSNLPDGDGNGGGVYEFGLTKS
                     SRTSLTISNDVYFDLGSQRGSGANANRGTINKIIGVRK"
     gene            31495..31884
                     /locus_tag="POB55_000207"
     CDS             31495..31884
                     /locus_tag="POB55_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2977 domain-containing protein"
                     /protein_id="HDI6499205.1"
                     /translation="MQILVNKRNEIISYAIIGGFEEGIDIENLPENFSQVFRPKAFKY
                     SNGEIVFNEDYSEEKDDLHQQIDSEEQNTVASDDILRKMVASMQKQVVQSTKLSMQVN
                     KQNALMAKQLVTLNKKLEEVKGETENA"
     gene            31877..32041
                     /locus_tag="POB55_000208"
     CDS             31877..32041
                     /locus_tag="POB55_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HDI6499206.1"
                     /translation="MLKLISPTFEDIKTWYQLKEYSKEDIAWYVDMEVIDKEEYAIIT
                     GEKYPENLES"
     gene            32087..32386
                     /locus_tag="POB55_000209"
     CDS             32087..32386
                     /locus_tag="POB55_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2951 domain-containing protein"
                     /protein_id="HDI6499207.1"
                     /translation="MFGFTKRHEQDWRLTRLEENDKTMFEKFDRIEDSLRTQEKIYDK
                     LDRNFEELRRDKEEDEKNKEKNAKNIRDIKMWILGLIGTILSTFVIALLKTIFGI"
     gene            32522..32824
                     /locus_tag="POB55_000210"
     CDS             32522..32824
                     /locus_tag="POB55_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HDI6499208.1"
                     /translation="MDAKVITRYIVLILALVNQFLANKGISPIPVDEESVSSIILTVV
                     ALYTTYKDNPTSQEGKWANQKLKKYKAESKYRKATGQAPIKEVMTPTNMNDTNDLG"
     gene            32835..34289
                     /locus_tag="POB55_000211"
     CDS             32835..34289
                     /locus_tag="POB55_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDI6499209.1"
                     /translation="MLITKNQAEKWFDNSLGKQFNPDLFYGFQCYDYANMFFMLATGE
                     RLQGLYAYNIPFDNKAKIEKYGQIIKNYDSFLPQKLDIVVFPSKYGGGAGHVEIVESA
                     NLNTFTSFGQNWNGKGWTNGVAQPGWGPETVTRHVHYYDNPMYFIRLNFPNNLSVGNK
                     AKGIIKQATTKKEAVIKPKKIMLVAGHGYNDPGAVGNGTNERDFIRKYITPNIAKYLR
                     HAGHEVALYGGSSQSQDMYQDTAYGVNVGNKKDYGLYWVKSQGYDIVLEIHLDAAGES
                     ASGGHVIISSQFNADTIDKSIQDVIKNNLGQIRGVTPRNDLLNVNVSAEININYRLSE
                     LGFITNKNDMDWIKKNYDLYSKLIAGAIHGKPIGGVVASEVKAPVKNEKNPPVPAGYT
                     LDKNNVPYKKEQGNYTVANVKGNNVRDGYSTNSRITGVLPNNTTITYDGAYCINGYRW
                     ITYIANSGQRRYIATGEVDIAGNRISSFGKFSAV"
BASE COUNT        13265 a         4710 c         6957 g         9637 t
ORIGIN      
        1 gcccgtttat gttggaggtt tataaatgct aaacagaaca atattagttg gtcgtttaac
       61 tagagaccca gaattaagaa ccactcaaag tggtgtaaat gtagcatcat tcacattagc
      121 agtaaaccgc acatttacga atgcacaagg agagcgcgag gcagacttta ttaatatcat
      181 cgtatttaaa aaacaagcag agaacgttaa taaataccta tctaaaggat cgttggcggg
      241 cgtagatggt aggttacaaa cgcggaacta tgaaaataag gaaggtcaac gtgtatacgt
      301 tacggaagtt gttgccgata gtattcaatt tttagaaccg aagaacacag atgataatca
      361 acaagattta taccaacaac aagcgcaaca aacacgtgga caatcgcaat attcaaataa
      421 caaaccagta aaagataatc cgtttgcgaa tgcaaattgt ccgattgaaa tagatgacaa
      481 tgatttacca ttctaattta accggtttga aagtgaggtg tgtatatgac tggttggata
      541 agtattgatc gctcaattca aaatcattgg ctatttaaag aaaagagaac attttcaaag
      601 tttgaagcat ggatatattt actcatggaa gcgaatcatt caaaggcaaa agtgcctatt
      661 ggaaaccaaa ttgtaaccgt agaaagagga caaagattaa catcgatttt gaccttgtct
      721 gaccttttta actggtcacg atttaaagtg aaaaccttcc ttgacttact cgagagtgat
      781 ggaatgttag aagtcaaaac aacatcaaaa tataccctta taaccattgt caattatgac
      841 ttttatcaaa gtgagcaggg aaggaaccaa catcaaaacg acatcaaacc aacatcaaaa
      901 caacatcagt caaacatcaa tccaacatca aaacaacatc aaaccaacac aaacaataat
      961 gataataaag ataataatga aaagaatgtg aataatgaga agaagaaggc agctgccttc
     1021 gacttcttcc aagataacgg attcggtttc ataactccat acaatttaga cgatttaaat
     1081 tattatcttg attcatttga aaatgattca gatgaaatag ttacggcatc acttaaaatc
     1141 gctaaagaca gaaacaaagt tacttgggga tatgctaaaa gcattttgaa tacatggctt
     1201 aatgcaaact tgaaatctat cgaacaagta cgtgcatttg aaaagcaaca aattgaaagc
     1261 aaaaagcaaa catacaaacc tcatgttaaa caatcaaaag aaaaaacgcc taaatggctc
     1321 acagacggca cgagagaaac gaaaacggcg gaagtagatg aaaaccttga gaaagacaga
     1381 gaagctttta ttaagcgtct aaatagcaaa tgggagtgat tgaaaatgga tgcatttgat
     1441 aaatactatc tatttgatca tgacggcaac aaaatgtttt cagttacacc acattttaaa
     1501 gatggtcggc atttagttgt tggaataaaa gaaacaaaat ctaatggtcg tcgttggtat
     1561 ttagacgatt atgaattaaa tacacttatt gataatgaac aaatggagtt aggacaccaa
     1621 acaaacttat ttgaatatat atgagggatt gcatggagat agaaattaaa tttaacgaag
     1681 tgtttaatgc gccgatgggg tcgcctcgac cgcgttttag cacaaaaggc agatacgtac
     1741 acacatacat gcctacaaac tatacaaaac ataaaaaata tttacaaagt caaatgccaa
     1801 ggctaaatct agaaaatgca ttaaaaatcg aattagcgtt ttactttact ccacctgaat
     1861 catggtcgaa gaaaaagaaa gctcaagcga ttggacaatt aaaagtgact aagccggata
     1921 tcgacaactt aattaaaacg gtattagatg cttgtaatgg ccatgtatgg aaagacgata
     1981 accaaattac agaaataact agctcaaagc gttatggaat tgagcccaaa ataatcatac
     2041 gaatagaaga aatataagag gtggataaaa tggcgagaaa agcaaggatt gtaacaataa
     2101 atgataaacc ttataggttc agtaaatttg aaatggaatt aatagaaagt cacggtataa
     2161 ccgctggaat ggtttctaag agagtaaaag acggttggga actacatgaa gcaatggacg
     2221 caccagaagg tacgcgttta agcgagtaca gagaaaagaa aacaatagaa agactggaac
     2281 aagctagact cgaacgcaaa ttggaaagaa agcgaaagag agaggctgag ctaagaagaa
     2341 agaagccaca cttgtttaat gtacctcaga aacatccaag aggacgttat gcgtgctacc
     2401 tgatggaaaa cgacatattc gtgaaagtta agaagtagat catgacagat aacgcacgta
     2461 aagaatactt aaaccaattt ttcggttcta agagatatct gtatcaggat aacgaaggag
     2521 tggcacatat ccatgtagta aacggcactt attactttca tgggcatatc gtaccaggtt
     2581 ggcaaggcgt gaaaaagaca tttgatacag cagaagagct cgaaacatat ataaagcaac
     2641 atggtttgga atacgaggag cagaagcaac taactttatt ttagaggaga tggaaatgat
     2701 gaataaccgc gaacaaattg aacagtccgt tataagtgct agtgcgtata acggtaatga
     2761 cacagaggga ttactaaaag agattgagga cgtatataag aaagcgcaag cgtttgatga
     2821 aatacttaag ggtttaccta atgctatgca agatgcactc aaagaagata ttggtcttga
     2881 tgaggcagta gggattatga cgggtcaagt ggtctataaa tatgaggagg agcaggaaaa
     2941 tgactaacac attaacaatt gatcagttac aagagttatt acaaatacaa aaggagttcg
     3001 acgatagaat accgacgcta aacttacgag atagcaagat tgcatatgta gttgaattct
     3061 ttgaatggtt taatacattg gaaacgttta agaactggaa gaagaaacca ggtaagccgt
     3121 tagacgtaca gttagatgaa ttggcggata tgttggcgtt tggattgagt attgcgaatc
     3181 aagtaggagt gtcatcagaa gagataaaag aagcgattga atcaagtttt aaaaataaag
     3241 aatttcacaa catgtttaat tttaaagata aagaatttgc tcaagatgcc gttgttagta
     3301 caccacagat aatatttaaa gaattttatc ccgaccaatt ggcaattgtt atagcgatag
     3361 acatagctta caacttatat tcaatcaacc aactcattga cgcatacaaa aagaaaatga
     3421 aaaggaatca tgaaagacaa gatggaacag cagacgcagg aaaaggatac gtgtaaagac
     3481 atattagatc gggtcaagga ggttttgggg aagtgacaca atacttagtc acaacattca
     3541 aagattcaac aggacgtaaa catacacaca taactaaagc taagagcaat caaagctata
     3601 cagttgttga ggcagagagt aaagaagaag caaaagagaa gtacgaggca caagttaaaa
     3661 gagatgcagt tattaaatta ggtcagttgt ttgaaaatat aagggagtgt gggaaatgaa
     3721 tcagctgaga attttattac atgacggtag tagtttgata ttacatgaag atgaattatt
     3781 taacgaaata gtatttgttt tggatgattt tagaaatgat gatgactatt taacgataga
     3841 aaaagattat ggcagagaac ttgtattgaa caaaggttat atagttggga ttaatgttga
     3901 ggaggcagac gatgattaac atacctaaaa tgaaattccc gaaaaagtac actgaaataa
     3961 tcaaaaaata taaaaataaa gcacctgaag aaaaggctaa gattgaagat gattttatta
     4021 aagaaattaa agataaagac agtgaatttt acagtcctac gatggctaat atgaatgaat
     4081 atgaattaag ggctatgtta agaatgatgc ctagtttaat tgatactgga gatgacaatg
     4141 atgattaaaa aacttaaaaa tatggatggg ttcgacatct ttattgttgg aatactgtca
     4201 ttattcggta tattcgcatt gctacttgtt atcacattgc ctatctatac agtggctagt
     4261 taccaacaca aagaattaca tcaaggaact attacagata aatataacaa gagacaagat
     4321 aaagaagaca agttctatat tgtattagac aacaaacaag tcattgaaaa ttccgactta
     4381 ttattcaaaa agaaatttga tagcgcagat atacaagcta ggttaaaagt aggcgataag
     4441 gtagaagtta aaacaatcgg ttatagaata cactttttaa atttatatcc ggtcttatac
     4501 gaagtaaaga aggtagataa acaatgatta aacaaatact aagactatta ttcttactag
     4561 caatgtatga gttaggtaag tatgtaactg agcaagtgta tattatgatg acggctaatg
     4621 atgatgtaga ggcgccgagt gattacgaaa aaatcagagc tgaagtttca tggtaatagc
     4681 tattatcatt tttgaattaa ttatattaat gtgtttagca atagcactgg aggtgttgta
     4741 aatatgtgga ttgtcatttc aatcgtttta gctatatttt tattgatctt gttaagtagc
     4801 atttctcata agatgaaaac catagaagca ttggagtata tgaattctta tcttttcaag
     4861 cagttagtaa aaaataatgg tgttgaaggt ttagaagatt atgaaaatga agttgaacga
     4921 attagaaaaa gattcaaaag ctaaagaggg ggctaaagcc ctctccagga ttaaaagaag
     4981 cggatatttt ttatttcgtt tttgtaaatt aaaactaatt tatgtgaaat tgtctttgaa
     5041 gtcttgataa ttaagaaggt tgaattttct gagattatat tagttacggg aaaggctttt
     5101 cctgagtgca ataaaattaa agttctcaag ttttcatttt cgtatttatc ataaatgatt
     5161 tcgcgtaaag ttttcaaaat ttgtcacctc tttaatttta ttaaattaat tatacaagaa
     5221 aggagccgaa tatgttagac aaagtcactc aaatagaaac aattaaatat gatcgtgatg
     5281 tctcatattc ttatgctgct agtcgtttat ccacatattg gactaatcac aatatggctt
     5341 ggtctgactt tatgcagaag ctagcacaaa cagttagaac taaagaagat ttaactgagt
     5401 acaataaaat gtctaagtct gaacaagcag atataaaaga tgttggcgga tttgtcggtg
     5461 gatatttaaa agaaggcaaa cgacgtgctg gtcaagtcat gaatcgttca atgctaacac
     5521 ttgatatcga ttatgctgct caagatatga ctgacatatt atctatgttt tatgattttg
     5581 catattgttt atattcaaca cataagcata gagagataag tccaagactg cgtttagtga
     5641 ttcctttaaa acgaaatgta aatgcagatg agtatgaagc tattgggcgt aaagtcgcag
     5701 atatcgttgg catggattac ttcgatgata caacttatca accacatagg ttaatgtatt
     5761 ggccttcaac tagcaatgat gcagaatttt tctttaccta tgaagattta cctttgttag
     5821 atccagataa aatattaaat gaatatgttg attggactga cacattagaa tggccaacgt
     5881 cttcaaagga agagagtaag actaaaagat tagcagataa gcaaggtgac ccagaagaaa
     5941 agccgggaat tgttggcgca ttttgtagag cctatacgat agaagaagct atatcaactt
     6001 ttattcctga cttatacgaa aaacattcta ctaaccgtta tacctatcat gaaggttcaa
     6061 ctgcaggtgg attggtgtta tacgaaaata acaagtttgc ctattcttat cataatacgg
     6121 atcccgttag cggtatgctt gtgaacagtt ttgatttagt acgcatacac ttatatggtg
     6181 ctcaagatga agacgctaaa acagatactc cggttaatcg actacctagt tataaagcaa
     6241 tgcagcaaag agcgcaaaat gatgaagttg ttaaaaagca attaattaac gacaaaatgt
     6301 ctgatgcaat gcaggatttc gatgaaatag taaatagcga tgatgcatgg tctgagacgt
     6361 tagaaattac ttcgaaaggt actttcaaag ctagtatccc aaatatagaa attatattgc
     6421 gtaatgatcc aaatttaaaa ggaaaaatag catttaatga atttacaaaa caaattgaat
     6481 gcttagggaa agtgccatgg aatactaatt ttaagacacg tcaatggcaa gacggtgatg
     6541 atagcagttt aagaagttat atcgaaaaga tttatgacat acaccattca ggtaaaacaa
     6601 aagatgccat tataagcgta gcaatgcaaa atgcttatca tccagtaagg gattatctaa
     6661 ataaaatatc gtgggatgga cataaacgtc ttgaaaagtt atttatcaaa tacttaggtg
     6721 ttgaagatac tgaagtgaat agaacaacta ccaaaaaagc attgactgct ggaattgctc
     6781 gagtaatgga gcctggatgt aaatttgact atatgcttac actttatggt cctcaaggtg
     6841 taggtaaatc tgctttgcta aaaaaattag gtggtgcatg gttttctgac agtttagttt
     6901 ctgttacagg taaagaagcc tatgaggcct tacaaggcgt ttggctaatg gaaatggcag
     6961 aacttgcagc tacaagaaaa gctgaagttg aagctattaa gcatttcata tctaaacaag
     7021 ttgaccggtt tcgtgttgct tatggacatt atattgaaga ttttccaagg caatgtattt
     7081 tcattggtac aactaataaa gttgatttct taagagatga aactggtgga agacgttttt
     7141 ggccaatgac tgtaaatcca gagagagttg aagtgaactg gtctaaacta accaaagaag
     7201 agatcgacca aatttgggca gaagctaaat attattatga acaaggagaa gagttattcc
     7261 tcaaccctga actagaagaa gaaatgcgtt caatacaaag caaacatact gaggaatctc
     7321 catatacagg cattattgat gaatatctta acacaccaat tcctagcaat tgggatgact
     7381 taactatctt tgaacgaaga cgattttatc aaggtgatgt tgatatgtta ccaacaggaa
     7441 atgtagatta cgttgaaaga aataaggtct gtgcgcttga agtgtttgtt gaatgttttg
     7501 gtaaagataa gggagatagt agaggatcta tggaaattag aaagatttca aacatcttaa
     7561 gacaattaga caattggtct gtatatgatg gtaataaaag tgggaaaatt cgatttggaa
     7621 aagattatgg tgtacagata gcttatgtaa gagatgaaag tttagaggat ttaatataat
     7681 aaatattgaa taaatataca ttttagagtg ttgtatcaga tgttgcatca ttttttgagt
     7741 gatgcaacac gtgagtgtaa aaagtaatcg taggtgttgc atcattttta gtgatgcaac
     7801 attgatgcaa caaatgatac aacacctctt tcccttctcg ctgtaaggtt caaacctgtt
     7861 tgtttccaat gttgcatcaa attcactata aagtttaaaa agtagtgtta gggagtaaag
     7921 aggtataggg gtaaccctct aacagctatt tttaaaagtt tggcaagaat tgatgcaaca
     7981 tcggaacaca aatataaatt ttgtatacaa ggtgaatata tgaaagaatc gacattagaa
     8041 aaatatttag tgaaagagat atcaaagcta aacggtttat gtttaaaatg ggttgcacct
     8101 ggaacaagag gtgtgccaga tagaattatt attatgccag aaggaaaaac atattttgta
     8161 gaaatgaagc aagaaaaagg aaagttgcat cctttacaaa aatatgtgca tagacaattt
     8221 gaaaatagag atcataaagt atatgtgtta tggaataaag aacaagtaaa tacttttatc
     8281 agaatggtag gtggaacatt tggcgattga cttcaaacca catagctatc aaaagtatgc
     8341 aatagataaa gtgatagata atgagaaata cggtctgttt ttagatatgg gtctagggaa
     8401 aacagtatca acacttacag catttagtga attgcagttg ttagacacta aaaaaatgtt
     8461 agttatagca cctaaacaag ttgctaaaga tacatgggtt gatgaagttg ataagtggaa
     8521 ccatttaaat catctgaaag tgtctttagt tttaggaaca cctaaagaaa gaaatgatgc
     8581 attaaacaca gaggctgata tctatgtaac caataaagaa aatactaaat ggttatgtga
     8641 tcaatataaa aaagaatggc catttgacat ggttgtgatt gatgaactgt ctacatttaa
     8701 aagtcctaag agtcaaaggt ttaaatctat taaaaagaaa ttaccactca ttaatagatt
     8761 tataggatta acaggaacac ctagtccaaa tagtttacag gatttatggg ctcaagttta
     8821 tttgatagac agaggtgaaa gacttgagtc ttcattcagt cgttatcgag aaaggtactt
     8881 taaaccaact catcaagtta gcgaacatat ttttaagtgg gagctaagag acggatctga
     8941 agaaaagata tataaacaaa tagaagatat atgtttaagc atgaaagcga aagattatct
     9001 ggatatgcct gacagagttg atactaaaca aacagtagtc ttatcagaaa aagaaagaaa
     9061 agtatatgaa gaattagaaa aaaactatat tttagaatcg gaagaagaag gaacagttgt
     9121 agctcaaaat ggggcatcat taagtcagaa actacttcaa ctatctaacg gcgcagttta
     9181 tacagatgag gaagatgtaa gacttataca tgataagaag ttagataagt tagaggaaat
     9241 tatagaggag tctcaaggcc aaccaatact attgttttat aacttcaaac acgataaaga
     9301 aagaatactt caaaggttta aggaagcaac cacattagag gattcaaact ataaagaacg
     9361 ttggaacagt ggagacatta agttgcttat agcacatcca gcaagtgcag gacatggatt
     9421 aaacttacaa caaggtgggc acattattgt ttggtttgga cttacatggt ccttggaatt
     9481 ataccaacaa gcaaatgcta gattatacag acaaggacaa aatcatacga ctattattca
     9541 tcatatcatg accgataaca caatagatca aagagtatat aaagctttac aaaataaaga
     9601 actaacgcaa gaagaattaa tgaaagctat taaagcaaga atagctaagc ataagtaatg
     9661 gaggtctaac atggggaaca caatatatga tatcaagcca ggaacattta aatatattga
     9721 atcagaaata tataatttaa atgagaacaa gaaagaaata aaaagattga ggttggaaat
     9781 acttaatcca acgaaggaac aagattccaa tattgtatat ggaccattac aaaaaggcga
     9841 accagttaga acaactgaac taatggcaac tagattatta actaataaga tgttacgaaa
     9901 ccttgaagaa atggtcgaag cagttgagag tgaatactta aagttgcctg aagatcataa
     9961 gaaagtaata aggctcaagt attggaataa agaaaagaag ttaaagatgg aacagatagg
    10021 acatgaatgc catatgcatc gtaatactgt tactactata cgaaagaact ttgttaaagc
    10081 ggtagcgtat catgcaggta tcaaataaca ttgtgcaaag attgtgcaaa aggcctacaa
    10141 atctgtagta atatgatagt atcggaaaga tgtataaagt tatctaaaag ttatacgaca
    10201 caagtacacg aggcacatcg ctatgcggtg tgtcttttgt tatgcaatca aagaggtgta
    10261 agagatgacc aagcacaata acatttataa gcatggtcgt aagtcatatc aatacgattg
    10321 gttctatcat tcaaaagcat ggaagaagtt aagggagata gcattagata gagataatca
    10381 tctttgtcaa atgtgtttac gcgaagatat tgtaacagat gcaaacatag tgcatcacat
    10441 tatttatgtt gatgaagatt ttaacaaagc tttagaatta gataatctaa tgtcagtttg
    10501 ttatggctgt cataacaaaa ttcatgcaaa tggtaatgac aaaagtaatc ttaagaaaat
    10561 tagagttcta aaaatttaaa taaaaaaaca tttaaataaa attttatagc cccctgccca
    10621 ttggcttaaa atgttttttc gccgggtacc ggcgggggcc cttcgcttgc aacgcggata
    10681 aacttttatg aaagggggtc tttatatgaa attaacaaaa aaacagctga aagaatatat
    10741 agaggattat aaaaaatctg atgacatatt aattaatttg tatatagaaa cgtatgaatt
    10801 ttattgtcgg ttaagagatg aacttaaaaa tagtgatttg atgatagagc atacaaacaa
    10861 ggctggtgcg agcaatattg ttaagaatcc attaagcata gaactgacaa aaacagttca
    10921 aacactaaat aacttactca agtctatggg tttaactgca gcacaaagaa aaaagatagt
    10981 tcaagaagaa ggtggattcg gtgactatta aagttttaaa tgaaccttca ccaaaactat
    11041 taacaacatg gtatgcagag caagtcactc aagggaaaat aaaaacaagc aaatatgtta
    11101 aaaaagaatg tgagagacac cttagatatc tagaaaatgg aggtaaatgg gtatttgatg
    11161 aagaattagc gcaccgtcct attcgattca tagaaaagtt ttgtaaacct tccaaaggat
    11221 ctaaacgtca acttgtatta caaccatggc aacattttat tattggcagt ttgtttggtt
    11281 gggttcataa agaaacaaaa ctgcgcaggt ttaaagaagc tttgatattt atggggcgaa
    11341 aaaatggtaa aacaactact atatctggtg ttgctaacta tgctgtttct caagatggag
    11401 aaaacggcgc tgaaatccat cttttagcaa acgtaatgaa acaagctaga atattattcg
    11461 atgaatctaa ggcgatgata aaagctagcc caaagcttga taaaaatttc agaacattaa
    11521 gagatgaaat ccattatgac gcaacgatat caaaaattat gccccaagca tcagatagcg
    11581 ataagttaga tggattgaat acacacatgg ggatttttga tgaaattcat gaatttaaag
    11641 actataaatt gatttcagtt ataaaaaact caagagctgc aaggttacaa cctcttctca
    11701 tctacattac gacagcaggg tatcaattag atggtccact tgttgatatg gtagaagcgg
    11761 gaagagacac cttagatcaa atcatagaag acgaaagaac tttttattat ttagcatctt
    11821 tggatgatga cgatgatatt aatgattcgt cgaactggat aaaagcaaat cccaacttag
    11881 gtgtctctat aaatttagat gagatgaaag aagagtggga aaaagctaag agaacaccag
    11941 ctgaacgtgg agattttata accaaaaggt ttaatatctt tgctaataat gacgagatga
    12001 gttttattga ttacccaaca ctccaaaaaa ataatgaaat tgtttcttta gaagagctgg
    12061 aaggcagacc atgcacgatt ggttatgatt tatcagaaac agaggacttt acagccgcgt
    12121 gtgctacttt tgcgttagat aatggtaaag ttgcagtttt atcgcattca tggattccta
    12181 agcacaaagt tgaatattct aacgaaaaaa taccctatag agaatgggaa gaagatggct
    12241 tattaacagt gcaagataag ccttatattg actaccaaga tgttttaaat tggataatta
    12301 agatgaatga gcattatgta gtagaaaaaa ttacttatga tagagcgaac gcattcaaac
    12361 taaatcaaga gttaaaaaat tacgggtttg aaacggaaga aacaagacaa ggagctttga
    12421 ccttgagccc tgcattgaag gatttaaaag aaatgttttt agatgggaaa ataatattta
    12481 ataataatcc tttaatgaaa tggtatatca ataatgttca gttgaaacta gacagaaacg
    12541 gaaactggtt gccgtctaag caaagcagat atcgtaaaat agatggcttt gcagcatttt
    12601 taaacacata tacagatatt atgaataaag ttgtttctga caagggtgaa ggaaacatag
    12661 aatttattag tattaaagat ataatgcgtt aaggaggtga atgttatcgc aaaagagaat
    12721 attgtcacac gcataaagaa aaaattgata gacaattgga ttgatcagtc agcttctaag
    12781 ctttatgact ttagcccatg gaaaaataaa tctttttggg gtgtaatcaa taatacgctt
    12841 gaaactaatg aaacgatatt ttcagctatt acaaagttat ctaattcgat ggctagtttg
    12901 cccttgaaaa tgtatgaaga ttataaagta gttaatacag aagtatctga tttacttaca
    12961 gtgtcaccga ataattctct gagcagtttt gattttatta atcaaattga aacaatcaga
    13021 aatgaaaaag gtaatgcata tgtgctaatt gaacgagaca tctatcatca accatcaaag
    13081 cttttcttat taaatccaga tgttgttgaa atgttaattg aaaaccaatc acgtgaactt
    13141 tattattcca ttcatgctgc aactggaaat aaattgattg ttcataatat ggacatgttg
    13201 cattttaaac acatcgtggc atctaatatg gtgcaaggca ttagtccgat tgatgtgttg
    13261 aagaatacaa ctgattttga taatgcagta agaaccttta atcttacaga aatgcaaaaa
    13321 cctgattctt tcatgcttaa atatggttcc aatgtaggta aagaaaaaag gcagcaagtg
    13381 ttagaagatt tcaaacagta ctatgaagaa aacggtggaa tattattcca agagcctggt
    13441 gttgaaatcg aaccgttacc taaaaaatat gtctctgaag atatagtggc aagcgagaat
    13501 ttaacaagag aaagagtagc taacgttttt caattgccct cagtattctt aaatgcaaga
    13561 tcaaatacaa atttcgcgaa aaatgaagag ttaaacagat tttacttgca gcatacctta
    13621 ttgccaatcg tcaaacagta tgaagaagaa tttaatcgga aactacttac taaaacagac
    13681 agagaaaaaa ataggtattt taaatttaac gttaaatctt atttaagggc tgatagtgca
    13741 acacaagcag aagtgtactt taaagcagtt cgtagtggtt actacactat aaatgacatt
    13801 agagagtggg aagatttacc accagttgaa ggtggagata agccgctaat aagcggtgat
    13861 ttatacccaa ttgacacgcc acttgaatta agaaaatctt tgaaaggtgg tgataaaaat
    13921 gtcaatgaaa gctaagtatt ttcaaatgaa aagaaaatca aaaagtaaag gtgaaatatt
    13981 tatttatggt gatattgtaa gtgataaatg gtttgaaagt gatgtaactg ctacagattt
    14041 caaaaataaa ctagatgaac taggagacat cagtgaaata gatgttcata taaattcatc
    14101 tggaggcagt gtatttgaag ggcatgcaat atacaatatg ctaaaaatgc atcctgcaaa
    14161 aattaatatc tatgtcgatg ccttagcggc atcaattgct agtgttatcg ctatgagtgg
    14221 tgacactatt tttatgcaca aaaatagttt tttaatgatt cataattcat gggttatgac
    14281 tgtaggtaat gcagaagaat taagaaagac agcggattta cttgaaaaaa cagatgctgt
    14341 tagtaattca gcttatttag ataaagcaaa agatttagat caagaacact taaaacagat
    14401 gttagatgca gaaacttggc ttactgcaga agaagccttg tctttcggct tgatagatga
    14461 aattttagga gctaatgaaa tagctgctag tatctctaaa gagcaatata agcgtttcga
    14521 gaacgtccca gaagatttaa agaaagatgt agacaaaatc actaaaattg atgatgtaga
    14581 tacatctgaa ttggttgaaa cacctaaaga aagtatgtca ctagaagaaa aagaaaaaag
    14641 agaaaaaatt aaacgcgaat gcgaaatttt aaaaatgaca atgaattatt aggaggaaat
    14701 gaaatgccga cattatatga attaaaacaa tccttaggta tgattggaca acaattaaaa
    14761 aataaaaatg atgaattgag tcagaaagca acagatccaa atattgatat ggaagacatc
    14821 aaacaactag aaacagaaaa agcaggttta caacaaagat ttaacattgt tgaaagacaa
    14881 gtgcaagaca ttgaagagaa agaaaaagcg aaagttaaag ataaaggaga agcttatcaa
    14941 tctttaagtg ataatgagaa gatggttaaa gctaaggcag agttttatcg tcacgcgatt
    15001 ttaccaaatg aatttgaaaa accttcaatg gaggcacaac gtttattaca cgctttacca
    15061 acaggaaatg attcaggtgg agataagctc ttaccaaaaa cactttctaa agaaattgtt
    15121 tcagaaccat ttgctaaaaa ccaattacgt gaaaaagctc gtctaactaa cattaaaggt
    15181 ttagagattc caagagtttc atacacttta gacgatgatg atttcattac agacgtagaa
    15241 acagcaaaag aattaaaagc aaaaggtgat acagtcaagt tcactactaa taaattcaaa
    15301 gtatttgctg caatttcaga tactgtaatt catggatcag atgtagattt agtaaactgg
    15361 gttgaaaacg cactacaatc aggattagca gctaaagagc gtaaagatgc cttagcagta
    15421 agtcctaaat ctggattaga acacatgtca ttttataatg gatctgttaa agaagttgag
    15481 ggagcagaca tgtatgatgc tattattaac gctttagcag atttacatga agattatcgt
    15541 gataacgcaa caatttatat gcgatatgca gattatgtca aaattattag tgttctttca
    15601 aatggaacaa caaatttctt tgacacacca gcagaaaaag tatttggcaa accagtagta
    15661 tttacagatg cagcagttaa acctattgtg ggagatttca attattttgg aattaactat
    15721 gatggaacaa cttatgacac tgataaagat gttaaaaaag gcgaatattt gtttgtatta
    15781 acagcatggt atgatcagca acgtacatta gacagtgcat tcagaattgc aaaagcaaaa
    15841 gaaaatacag gtccattacc cagctaagcc ccaaaaggtt aatgtaacag ctaaggctaa
    15901 atcagctgta atatcagccg aataggggtg atgaaatgag tttagaagaa attaaattgt
    15961 ggttgagaat tgactataat ttcgaaaatg atttaattga aggtctcatt caatcggcta
    16021 agtctgaatt actattaagt ggggttccag attatgacaa agatgacttg gaatacccgc
    16081 ttttttgtac agcgattaaa tatatcattg caagagatta tgaaagtcgt ggatactcaa
    16141 atgaccaatc tagaagcaag gtgtttaatg aaaaaggatt gcaaaaaatg attttgaaat
    16201 taaaaaagtg gtaggtgatt tttaaatgga atttaatgaa tttaaagatc gcgcgtattt
    16261 ttttcaatat ataaacaaag gaccatatcc agatgaagag gaaaaaatga aattgtatag
    16321 ttgcttttgt aaaatttata atccttctat gaaagataga gaaattttaa aagcgactga
    16381 atcaaaatca ggattaacca taattgtcag gtcttctaaa actgaatatc taccacaaac
    16441 aaatcactta gttaaaattg acagtgcatt atattccgat aaattattca acattgtaga
    16501 aataagaatt gatacaccag atattggcta taatacagtg gttttatcag aaaaatgagt
    16561 gtagaaatta aagggatacc tgaagtgttg aataaattag aatcggtata cggtaaacaa
    16621 gcaatgcagg ctaagagtga taaagcttta aatgaagcat ctgaattttt tataaaggct
    16681 ttaaagaaag agttcgagag ctttaaagat acgggtgcca gtatagaaga aatgactaaa
    16741 tctaagcctt atacaaaagt tggcagtcaa gaaagggctg ttttaattga atgggtaggc
    16801 cctatgaatc gcaaaaacat tattcacttg aatgaacatg gttatacaag agatggaaaa
    16861 aaatatacac caagaggttt tggagttatt gcaaaaacat tagctgctag cgaacgtaag
    16921 tatagagaaa ttataaaaaa ggagttggcc agataaatga atatattaaa caccataaaa
    16981 ggaattttat tatctgatgc agagctcaaa acacatataa attctagaat atactattac
    17041 aaagtcactg aaaacgctga aacttccaaa ccctttgttg ttattacacc tgtttatgat
    17101 ttgccttcag actttatgtc tgataaatat ctcagtgaag aatacttaat tcaaatagat
    17161 gtagaatctt caaataatca gaaaacaatt gatataacaa aacgaataag atacctgtta
    17221 tatcaacaaa atttaattca agcatctagt cagttagatg cttattttga agaaactaaa
    17281 cgttatgtga tgtcgagacg atatcaaggc atacccaaaa atatatatta taaaaatcag
    17341 cgcatcgaat aggtgtgctt tttaattttt aaggaggaaa taagcaatgg cagaaggaca
    17401 aggttcttat aaagtaggtt ttaaaagatt atacgttgga gtttttaacc cagaagcaac
    17461 aaaagtagtt aaacgcatga catgggaaga tgaaaaaggt ggtacagttg acctaaatat
    17521 cacaggttta gcaccagatt tagtagatat gtttgcatct aacaaacgtg tatggatgaa
    17581 aaaacaaggt actaatgaag ttaagtctga catgagtatt ttcaatattc caagtgatga
    17641 tttaaacaca gttattggac gtactaaaga taaaaatggt acatcttggg taggagagaa
    17701 tacaagagca ccgtatgtaa cagtaattgg cgaatcggaa gatggtttaa caggtcagcc
    17761 ggtatatgta gccttactta aaggtacttt tagtttagat tcaattgaat ttaaaacacg
    17821 aggtgaaaaa gcagaagccc cagaacctac aaaattaaca ggtgactgga tgaatagaaa
    17881 agttgatgtt gatggaacgt cacaaggtat tgtatacggt tatcatgaag gtaaagaagg
    17941 agaagcagaa ttcttcaaaa aagtattcgt tggatacacg gacagtgaag atcattcaga
    18001 ggattctgca ggttcgttac ccagctaatc cccaaaatgt tgaagtagca gttaattcaa
    18061 aatctgcaac agtttcagca gaataggggc tttcaaaata aatcaaagga gaataattta
    18121 tgactaaaac tttaaaggtt tataaaggag acgacgtcgt agcttctgaa caaggtgaag
    18181 gcaaagtgtc agtaacttta tctaatttag aagcggatac aacttatcca aaaggtactt
    18241 accaagtggc atgggaagaa aatggtaaag aatctagtaa agttgatgta cctcaattca
    18301 aaaccaatcc aattctagtc tcaggcgtat catttacacc agaaactaaa tcaattatgg
    18361 taaataccga tgacaatgtt gagccaaaca ttgcaccaag cacagcaacg aataaaatat
    18421 tgaaatatac aagtgaacat ccagaatttg ttactgtaga tgaaaataca ggagcaattc
    18481 acggtgtagc tgaaggtact tcagtaatca ctgctatgtc tactgatgga agcgataagt
    18541 caggacaaat ttcagtgaca gtaacaaacg gatagggatt taaggcgcag tatatctgcg
    18601 tcttttttat ttgaataaaa ggagctaata caatgattaa atttgaaatt aaagatcgta
    18661 aaacaggaaa aacagagagc tatacaaaag aagatgtaac aatgggcgaa gcagaaaaat
    18721 gctatgagta tttagaatta gtaaatcaag agaataaaaa agaagcacct aacgcaacaa
    18781 aaatgagaca aaaagagcga cagttattag tagatttatt taaagatgaa ggattgactg
    18841 aagaagatgt tctgaacaag atgagtacta aaacttatac aaaagcctta caagatatat
    18901 ttcgagaaat caatggtgaa gatgaagaag attcagaaac tgaaccagaa gagatgggaa
    18961 agacagaaga acaatctcaa taaaagacat tttatcgaac attaagaaaa tacaacgttt
    19021 ctgtatggag cagtatgggt ggacattaac tgaagtcaga aaacagccgt atgtaaaact
    19081 tttagaaata cttaatgaag agaataaaga agagactgaa gaaaaacaaa gtgaacaaaa
    19141 agtcattaca ggtacggatt taagaaaact ttttggaagc tagaaaggag gttaatatga
    19201 atgaaaaagt agaaggcatg accttggagc tgaaattaga ccatttaggt gtccaagaag
    19261 gcatgaaagg tttaaagcga caattaggtg ttgttaatag tgaaatgaaa gctaatctgt
    19321 cagcatttga taagtctgaa aaatcaatgg aaaaatatca ggcgagaatt aaggggttaa
    19381 atgataggct taaagttcaa aaaaagatgt attctcaagt agaagatgag cttaaacaag
    19441 ttaacgctaa ttaccaaaaa gctaaatcca gtgtaaaaga tgttgagaaa gcatatttaa
    19501 agttagtaga agccaataaa aaagaaaaat tagctcttga taaatctaaa gaagccttaa
    19561 aatcatcgaa tacagaactt aaaaaagctg aaaatcaata taaacgtaca aatcaacgta
    19621 aacaagatgc gtatcaaaaa cttaaacagt tgagagatgc agaacaaaag cttaagaata
    19681 gtaaccaagc tactactgca caactaaaaa gagcaagtga cgcagtacag aagcagtccg
    19741 ctaagcataa agcacttgtt gaacaatata aacaagaagg caatcaagtt caaaaactaa
    19801 aagtgcaaaa tgacaatctt tcaaaatcaa atgataaaat tgaaagttct tacgctaaaa
    19861 ctaatactaa attaaagcaa acagaaaaag aatttaatga tttaaacaat actattaaga
    19921 atcatagcgc taatgtcgca aaagctgaaa cagctgttaa taaagaaaaa gctgctttaa
    19981 ataatttgga gcgttcaata gataaagctt catccgaaat gaagactttt aacaaagaac
    20041 aaatgatagc tcaaagtcat ttcggtaaac ttgcaagtca agcggatgtc atgtcaaaga
    20101 aatttagttc tattggagac aaaatgactt ccctgggacg tacaatgacg atgggcgtat
    20161 ctacaccaat tactttaggg ttaggtgcag cattaaaaac aagtgcagac tttgaaggcc
    20221 aaatgtctcg agttggagcg attgcgcaag caagcagtaa agacttgaaa agcatgtcta
    20281 atcaagcagt tgacttagga gctaaaacca gtaaaagtgc taacgaagtt gctaaaggta
    20341 tggaagaatt ggcagcttta ggctttaatg ccaaacaaac aatggaggct atgccaggtg
    20401 ttatcagtgc agcagaagca agtggtgcag aaatggctac aactgcaact gtaatggctt
    20461 cagcgattaa ctctttcggt ttaaaagcat ctgatgcaaa tcatgttgct gatttacttg
    20521 cgagatcagc aaatgatagt gctgcagata ttcagtacat gggagatgca ttgaagtatg
    20581 ctggtactcc tgcaaaagca ttaggagttt caatagagga cacttccgca gcaattgaag
    20641 ttttatctaa ctcaggttta gagggttctc aagcaggtac tgccctaaga gcttcattta
    20701 tcaggctagc taatccaagt aaaaatacag ctaaggaaat gaaaaaatta ggtattcatt
    20761 tgtctgatgc taaaggtcaa tttgttggca tgggtgaatt gattagacag ttccaagata
    20821 atatgaaagg catgacgaga gaacaaaaac tagctacagt ggctacaata gttggtactg
    20881 aagcagcaag tggattttta gccttgattg aagcgggacc agataaaatt aatagctata
    20941 gtaaatcctt aaagaattcc aatggcgaaa gtaaaaaagc agcagatttg atgaaagata
    21001 atctcaaagg cgctctggaa caattaggtg gcgcttttga atcattagca atcgaagtcg
    21061 gtaaagattt aacgcctatg attagagcag gagcggaagg tttaacaaaa ttagttgatg
    21121 gatttacaca tctccctggt tgggttagaa aagcttcagt aggattagca ctttttggtg
    21181 cagcaattgg acctgcagtt cttgctggag ggttattaat acgtacagtt ggaagtgctg
    21241 ctaaaggata tgcgtcatta aatagacgta ttgctgaaaa tacaatcctt tcaaatacta
    21301 attcaaaagc aatgaaatct ttaggtcttc aaacattatt tcttggttct acaacaggaa
    21361 aaacgtcaaa aggctttaaa gggttagccg gagctatgat gtttaattta aaacctataa
    21421 atgttttgaa aaattctgca aagctagcaa ttttaccgtt caaacttttg aaaaacggtt
    21481 taggattagc tgcaaaatct ttatttgcag taagtggagg cgcaagattt gcgggtgtag
    21541 ccttaaggtt tttaacagga cctataggtg ctacaataac tgctattaca attgcgtata
    21601 aagtttttaa aaccgcatat gatcgtgtgg aatggttcag aaacggtatt aacggtttag
    21661 gagaaactat aaagtttttt ggtggtaaaa ttattggcgg cgctgttaga aagctaggag
    21721 agtttaaaaa ctatcttgga agtatcggca aaagcttcaa agaaaagttt tcaaaagata
    21781 tgaaagatgg ttataaatca ttaagcgacg atgaccttct caaagtagga gtcaacaagt
    21841 ttaaaggatt tatgcaaacc atgggcacag cttctaaaaa agcgtctgat actgtaaaag
    21901 tgttagggaa aggtgtttca aaagaaacag aaaaagcttt agaaaaatat gtgcattatt
    21961 ctgaagaaaa tagcagaatc atggaaaaag tacgtttaaa ctcgggtcag atatcagaag
    22021 acaaagcaaa aaaacttttg aaaattgaaa cggatttatc taataacctt atagctgaaa
    22081 tagaaaaaag aaataaaaag gaactcgaaa aaactcaaga acttattgat aagtatagtg
    22141 cattcgatga acaagaaaag caaaacattt taactcgaac taaagaaaaa aatgacttgc
    22201 gaattaaaaa agagcaagaa ctcaatcaga aaatcaaaga attgaaagaa aaagctttga
    22261 gtgatggtca gatttcagaa aatgaaagaa aagaaattga aaagcttgaa aatcaaagac
    22321 gtgatatcac tgttaaagaa ttgagtaaga ctgaaaaaga gcaagagcgt attttagtaa
    22381 gaatgcaaag aaacagaaat gcttattcaa tagacgaagc gagcaaagca attaaagaag
    22441 cagaaaaagc aagaaaagca agaaaaaaag aagtggacaa gcaatatgaa gatgatgtca
    22501 ttgctataaa aaataacgtc aacctttcta agtctgaaaa agataaattg ttagctattg
    22561 ctgatcaaag acataaagat gaagtaagaa aagcaaaatc taaaaaagat gctgtagtag
    22621 atgttgttaa aaagcaaaat aaagatattg ataaagaaat ggatttatcc agtggacgtg
    22681 tatataaaaa tactgaaaag tggtggaatg gccttaaaag ttggtggtct aactttagag
    22741 aagaccaaaa gaaaaaaagc gataaatacg ctaaagaaca agaagaaaca gctcgtagaa
    22801 acagagaaaa tataaagaaa tggtttggaa atgcttggga cggcgtaaaa agtaaaactg
    22861 gcgaagcctt tagtaaaatg ggcagaaatg ctaatcattt tggcggcgaa atgaaaaaaa
    22921 tgtggagcgg aatcaaaggg attccaagca aattaagttc aggttggagc tcagccaaaa
    22981 gttctgtagg ataccacact aaggctatag ctaatagtac tggtaaatgg tttggaaaag
    23041 cttggcaatc tgttaaatcg acaacaggaa gtatttacaa tcaaactaag caaaagtatt
    23101 cagatgcttc agataaagct tgggcgcatt caaaatctat ttggagaggc acatcaaaat
    23161 ggtttagcaa tgcatataaa agtgcaaagg gctggctaac ggatatggct aataaatctc
    23221 gcgcgaaatg ggataatatt tctagtacag cttggtcgaa tgcaaaatcc gtttggaaag
    23281 gaacatcgaa atggtttagt aactcataca aatctttaaa agattggact ggggatatgt
    23341 attcaagagc ccacgatcgt tttgatgcaa tttcaagttc ggcatggtct aacgctaaat
    23401 cagtatttaa tggttttaga aaatggctat ccaaaacata tgattggatt agagatattg
    23461 gtaaagacat gggaagagct gcggctgatt taggtaaaaa tgttgctaat aaagctattg
    23521 gcggtttgaa tagcatgatt ggcggtatta ataaaatatc taaagccatt actgataaaa
    23581 atctcatcaa gccaatacct acattgtcta ctggtacttt agcaggaaag ggtgtagcta
    23641 ccgataattc gggagcatta acgcaaccga catttgctgt attaaatgat agaggttctg
    23701 gaaacgcccc aggtggtgga gttcaagaag taattcacag ggctgacgga acattccatg
    23761 caccccaagg acgagatgtg gttgttccac taggagttgg ggatagcgta ataaatgcta
    23821 atgacactct gaagttacag cgtatgggtg ttttaccaaa gtttcatgga ggtacgaaaa
    23881 agaaagattg gctagaccaa cttaaaggta atataggtaa aaaagcagga gaatttggag
    23941 ctacagctaa aaacacagcg cataatatca aaaaaggtgc agaagaaatg gttgaagcag
    24001 caggcgataa aatcaaagat ggtgcatctt ggttaggcga taaaatcggc gatgtgtggg
    24061 attacgtaca acatccaggg aaactagtaa ataaagtaat gtcaggttta aatattaatt
    24121 ttggaggcgg agctaacgct acagtaaaaa tagctaaagg cgcatactca ttgctcaaaa
    24181 agaaattagt agacaaagta aaatcgtggt ttgaagattt cggtggtgga ggcgatggaa
    24241 gctatctatt tgaatatcca atctggcaaa gatttggacg ctacacaggt ggacttaact
    24301 ttaatggcgg tcgtcactat ggtatagact ttggtatgcc ttctggaaca aacgtttatg
    24361 ccgttaaagg tggtatagca gataaggtat ggactgatta cggtggcggt aattctatac
    24421 aaattaagac tggtgctaat gaatggaact ggtatatgca tttatctaag caattagcaa
    24481 gacaaggcca acgtattaaa gctggtcaac tgatagggaa atcaggtgct acaggtaatt
    24541 tcgttagagg agcacactta catttccaat tgatgcaagg gtcacatcca gggaatgata
    24601 cagctaaaga tccagaaaaa tggttgaagt cacttaaagg tagtggcgtt cgaagtggtt
    24661 caggtgttaa taaggctgca tctgcttggg caggcgatat acgtcgtgca gcaaaacgaa
    24721 tgggtgttaa tgttacttcg ggtgatgtag gaaatattat tagcttgatt caacacgaat
    24781 caggaggaaa tgcaggtata actcaatcta gtgcgcttag agacatcaac gttttacagg
    24841 gcaatccagc aaaaggattg cttcaatata tcccacaaac atttagacat tatgctgtta
    24901 gaggtcacaa caatatatat agtggttacg atcagttatt agcgttcttt aacaacagat
    24961 attggcgctc acagtttaac ccaagaggtg gttggtctcc aagtggtcca agaagatatg
    25021 cgaatggtgg tttgattaca aagcatcaac ttgctgaagt gggtgaagga gataaacagg
    25081 agatggttat ccctttaact agacgtaaac gagcaattca attaactgaa caggttatgc
    25141 gcatcatcgg tatggatggc aagccaaata acatcactgt aaataatgat acttcaacag
    25201 ttgaaaaatt gttgaaacaa attgttatgt taagtgataa aggaaataaa ttaacagatg
    25261 cattgattca aactgtttct tctcaggata ataacttagg ttctaatgat gcaattagag
    25321 gtttagaaaa aatattgtca aaacaaagtg ggcatagagc aaatgcaaat aattatatgg
    25381 gaggtttgac taattaatgc aatcttttgt aaaaatcata gatggttaca aggaagaagt
    25441 aataacagat tttaatcagc ttatattttt agatgcaagg gctgaaagtc caaacaccaa
    25501 tgataacagt gtaactatta acggagtaga tggtatttta ccgggcgcaa ttagttttgc
    25561 gcctttttca ttagtattaa ggtttggcta tgatggtata gatgttatag atttaaattt
    25621 atttgagcat tggtttagat ctgtgtttaa tcgcagacat ccttattatg ttattacttc
    25681 tcaaatgcct ggtgttaaat atgcagtgaa tacagctaat gttacatcta atttaaaaga
    25741 tggttcttca actgaaattg aagtaagttt aaatgtttat aaagggtatt ctgaatcagt
    25801 taattggacc gatagcgagt tcttattcga ctctaattgg atgtttgaaa atggaattcc
    25861 tcttgatttc acacctaaat atactcatac atcaaatcaa tttactattt ggaacggttc
    25921 tactgatacg ataaatccac gattcaagca cgatttgaaa atattaatta atttaaatgc
    25981 gagtggagga tttgaactgg ttaactatac aacaggtgat atttttaagt acaacaaaag
    26041 tatagataaa aacactgatt ttgttttaga tggtgtgtat gcatatcgag atataaatag
    26101 agtgggaatt gatacaaata gaggcattat aacattagcg ccaggtaaaa atgaatttaa
    26161 gattaaagga gacgtcagtg atattaaaac tacatttaag tttcctttta tttataggta
    26221 ggtgatttaa tggattatca tgatcattta tcagtaatgg attttaatga attgatttgt
    26281 gaaaatttac tagatgtaga ttatggttct tttaaagaat attatgaact gaatgaagct
    26341 aggtacatca cctttacagt ttatagaact actcataata gttttgtttt tgatttattg
    26401 atttgtgaaa acttcataat ttatcatggt gaaaaatata caattaagca gacagcgcca
    26461 aaggttgaag gtgataaagt ttttattgaa gttacggcat atcacataat gtatgaattt
    26521 caaaatcact cagtggaatc aaataagctt gatgacgaca gtagcgaaac tggtaaaacg
    26581 ccagaatact ctttagatga gtacttaaga tatggatttg caaatcaaaa aacttcggtc
    26641 aaaatgacct ataaaataat tggagatttt aagcgaaaag taccgattga cgaattaggt
    26701 aacaaaaacg gcttagaata ctgtaaagaa gcggtagacc tgtttggctg tataatttac
    26761 ccaaatgata cagagattgg tttttattct cctgaaacat tttatcaaag aagcgagaaa
    26821 gtgattcgat atcaatataa tactgatact gtatctgcaa ctgtcagtac attggaatta
    26881 agaacagcta taaaagtttt tggaaaaaag tatacagctg aggaaaagaa aaattataat
    26941 cctattagaa caactgacat taaatattca aatggtttta taaaagaagg tacttatcgt
    27001 accgaaacaa ttgggtctaa agctactatt aactttaatt gcaagtatgg taatgaaaca
    27061 gttagattta caataaaaaa gggctctcaa ggtggaatat ataagttgat tttagacggc
    27121 aagcaaatta agcaaatttc ttgttttgct aagtcggttc agtctgaaac aatagattta
    27181 ataaaaaata ttgataaagg caagcacgtt ttagaaatga tatttttagg agaagacccc
    27241 aaaaatagaa ttgatatatc ttcaaataaa aaagctaagc cttgtatgta tgttggaact
    27301 gaaaaatcaa cagtcttaaa tttaattgct gataattcag gtcgcaatca atacaaagca
    27361 attgtcgact acgtcgcaga tagtgcaaag cagtttggga ttcgatatgc taatacgcaa
    27421 acaaatgaag atatcgaaac acaggataag ctgttagaat ttgcaaaaaa gcaaataaat
    27481 gatactccta agactgaatt agatgttaat tatataggtt atgaaaaaat agagccaaga
    27541 gatagcgtat tttttgttca tgaattaatg ggatataaca ctgaattaaa ggttgttaaa
    27601 cttgataggt cacatccatt tgtaaacgca atagatgaag tgtctttcag caatgaaata
    27661 aaagatatgg tacaaattca acaagcactt aacagacgag ttattgcaca agataataga
    27721 tataactatc aagcaaatcg tataaatcat ttatacacta gtactttgaa ttctcctttc
    27781 gagacaatgg atatagggag tgtattaata taatggcaac agaagaagtt aaaatcaaag
    27841 cgctacttga aaacgataaa cagtactttc cagctacaca ctggaaagct ataaatggga
    27901 taccttatgc aggcagtagt gatattgatg gattgcctca agacggtatc atttcggtag
    27961 atgataaaaa taaattagat aaattaaaaa taggcgaagc aggaattatt caaaatagca
    28021 ttgtacagaa atccccaaac ggtaaattgt ggaaaataac agttgacgat agtgggaaac
    28081 ttggtacagt gctattttat tagaaaggaa ggtgcattat ggaaaatttg tatttaataa
    28141 aggatttggg agctttagca ggtcgagatt atagagctaa agaaattcaa aacctgcaaa
    28201 gaatagagca atttgcgctt ggcttgacaa cagagtttaa gttgcatcag aaagctaaaa
    28261 caatgcaaca cttcgctgag caaatttatt ataatggtag atcgcaagca gcagtaaaca
    28321 aatctttaca aagtcaaatt aacgcacttg ttgtggcacc acgtaataac agtgctaatg
    28381 agattgttca agctcgagtt aatgtaaacg gcgaaacctt tgacacatta aaagaacatt
    28441 tagacgattg ggaaaccaaa actcaaatta ataaagagga aactataaga gaattaaata
    28501 agaccaaaca agaaattctt gatatcgagt atcgttttga acctgataag caagagtttt
    28561 tatttgtgac agaacttgca cctcttacaa atgcagtaat gcaatccttc tggtttgata
    28621 atagaacagg catagtatac atgacacaag ctagaaataa tggctatatg ctaagtcgtt
    28681 taagacctaa tggtcaattt atagacagct cattgattgt aggtgggggt catggtacac
    28741 ataacggtta tagatatatt gatgatgagt tatggattta tagttttatc ttaaatggta
    28801 ataatgagaa tacattagtt cgtttcaagt atacgcctaa tgtggaaatt agctatggca
    28861 agtatggtat gcaagatgta tttacaggac acccagaaaa accctacatc acccctgtca
    28921 taaatgaaaa agaaaataaa attctataca gaattgagag acctagaagt cagtgggaac
    28981 ttgaaaactc aatgaattat atagagataa gaagtttaga cgatgttgat aaaaatattg
    29041 ataaagtttt gcataaaatc agtatcccta tgagactaac aaacgaaacc caaccaatgc
    29101 agggtgtgac ttttgatgaa aaatacttgt attggtatac aggagacagt aatccaaata
    29161 atagaaacta tttaacggct ttcgatttag aaacaggaga agaagcgtat caggttaatg
    29221 ctgactatgg tggaacacta gattcatttc ctggcgaatt tgcggaagca gaaggtttgc
    29281 aaatatacta tgacaaagat agtggtaaaa aagctttgat gctaggtgtt actgtcggtg
    29341 gtgatggaaa tagaacacat cgtattttca tgattgggca aagaggtatt ttagaaatac
    29401 ttcactcaag aggcgttcct tttatcatga gtgacacagg tggtagagtt aaacctttac
    29461 caatgaggcc tgataaactt aagaatcttg ggatgttaac agagccaggt ctttactatt
    29521 tatacactga tcatacagtt caaatcgatg atttcccatt accaagagaa tggcgtgatg
    29581 caggttggtt cttggaagtt aagccaccac aaactggcgg tgatgtaatt cagatattga
    29641 cgcgtaatag ttatgcaagg aatatgatga cttttgaaag ggtgctttct ggaagaactg
    29701 gagacatttc ggactggaat tatgtgccta aaaatagtgg taaatgggag agagtacctt
    29761 cattcatcac aaaaatgtca gatattaaca tagtaggcat gtcgttttat ttaactacgg
    29821 atgatacaaa acgttttaca gattttccaa ctgaacgtaa aggggtagct ggttggaact
    29881 tatatgtaga agcttcaaac acaggtggct ttgttcatag gctagttcgt aatagtgtta
    29941 cagcatctgc tgagatacta ttgaaaaatt atgatagtaa aacaagttca gggccatgga
    30001 ctttacacga agggagaatt ataagttaat gagtaattta gagaaatctg tagctataaa
    30061 tttagaaaac acagcgcatt atgaaaatat ttcaaatcta gatataactt ttagaacagg
    30121 agagagtgat tcttctgttc ttctttttaa tatcattaaa aataatcaac cgttattact
    30181 gagtgaagaa aatatcaaag cacgaatagc gattcgaggt aaaggagtaa tggtagttgc
    30241 tccactagaa atattagatc catttaaagg tattttaaaa tttcaattac ctaatgatgt
    30301 aattaaaaga gatggaagtt atcaagctca agtttcggtt gcagaattag gtaattcaga
    30361 cgtggtagtt gtagagagaa ctatcacatt taacgttgaa aaaagtttgt ttagcaagat
    30421 tccctctgaa acaaaactac actatattgt tgagtttcaa gaattagaaa aaactattat
    30481 ggatcgcgcg aaagcaatgg acgaggctat aaaaaatggt gaggattatg cgagtctgat
    30541 tgaaaaagct aaagaaaaag gtctatcaga tattcaaata gcaaaatctt caagtattga
    30601 tgaattaaag caacttgcta atagccgtat atctgatttg gaaaataaag cgcaagcata
    30661 ttcaagaaca ttcgatgagc aaaagcgata tatggatgag aaacatgaag ccttcaagca
    30721 gtcagtgaat agtggtggtt tagtcacaag tggttctact tcaaattggc aaaaagctaa
    30781 gattactaaa gatgatggta agataatgca gattactgga tttgatttta ataatccaga
    30841 acaaagaata ggtgattcaa cccaatttat ttatgtttcg caagctataa attatccaag
    30901 agatgttagt actaacggta ctgtcgaata tttagtagta acttcagatt acaagcgtat
    30961 gacttatcga ccgaacggta caaataaagt gtttgttaaa agaaaagaag cgggttcatg
    31021 gtctgagtgg tcagaattag ctattaatga ttacaataca ccttttgaaa ctgttcaaag
    31081 tgcccaatca aaagctaata tggccgaaag taacgctaaa ttatacgcag atgacaagtt
    31141 taataaaagg tattcggtta tttttgatgg aacagcaaat ggtgtgggct ctacattgta
    31201 cttaaatgag agtttagacc aatttatttt attaattttt tatgggactt ttccaggtgg
    31261 tgactttaca gagtttggca gtccttttgg aggaggaaag atttcattga atccctcaaa
    31321 tcttccagat ggtgatggaa atggtggagg tgtttatgag tttggattaa ctaaatctag
    31381 tcgtacatct ttaactatat caaacgatgt ctatttcgac ttaggaagtc aaagaggctc
    31441 tggtgcgaac gcaaatagag ggacaattaa caaaattata ggagtgagaa aataatgcaa
    31501 atattagtta acaagcgtaa tgagataatt tcatacgcta tcattggcgg ctttgaagaa
    31561 ggtattgata ttgaaaattt accagaaaat ttctctcaag tttttagacc taaagccttt
    31621 aaatattcaa atggggaaat agtttttaac gaagattatt cagaagaaaa agatgacttg
    31681 catcaacaga ttgacagtga agaacaaaac acagtcgctt ctgatgacat cttacgaaaa
    31741 atggttgcta gtatgcagaa acaagttgtt caaagtacaa agttatcgat gcaagttaat
    31801 aagcaaaatg cactaatggc aaaacaactt gtgacactta ataaaaaatt agaagaggtt
    31861 aaaggagaga ctgaaaatgc ttaaattaat ttcaccaaca ttcgaagata ttaaaacatg
    31921 gtatcaattg aaagaatata gtaaagaaga tatagcgtgg tatgtagata tggaagttat
    31981 agataaagag gaatatgcaa ttattacagg agaaaagtat ccagaaaatc tagagtcata
    32041 ggttataatc ttatggcttt ttaatttgaa taaagtgggt ggtgtaatgt ttggatttac
    32101 caaacgacac gaacaagatt ggcgtttaac gcgattagaa gaaaatgata agactatgtt
    32161 tgaaaaattc gacagaatag aagacagtct gagaacgcaa gaaaaaattt atgacaagtt
    32221 agatagaaat ttcgaagaac taaggcgtga caaagaagaa gatgaaaaaa ataaagagaa
    32281 aaatgctaaa aatattagag acatcaagat gtggattcta ggattaatag ggacgattct
    32341 aagtacattt gttatagcct tgttaaaaac tatttttggc atttaaagga ggtgatcacc
    32401 atgcttaagg gaattttagg atatagcttt tggtcgtgtt tctggtttgg taagtgtaag
    32461 taatatttaa gagtcagtgc ttcggcactg gctttttatt ttggataaaa ggagcaaaca
    32521 aatggatgca aaagtaataa caagatacat cgtattgatc ttagcattag taaatcaatt
    32581 cttagcgaat aaaggtataa gtccgatacc agtagatgaa gaaagtgttt catcgattat
    32641 cttaacagtt gttgctttat atactacata taaagataat ccaacatctc aagaagggaa
    32701 atgggcgaat caaaaattaa agaaatataa agctgaaagt aaatatagaa aagcaacagg
    32761 gcaagcgcca attaaagaag taatgacacc tacgaatatg aacgacacaa atgatttagg
    32821 gtaggtgttg accaatgttg ataacaaaaa accaagcaga aaaatggttt gataattcat
    32881 tagggaagca gttcaatcct gatttgtttt atggatttca gtgttatgat tacgccaata
    32941 tgttctttat gttagcgaca ggcgaaaggc tgcaaggttt atatgcttat aatatcccgt
    33001 ttgataataa agcaaagatt gaaaaatatg gtcaaataat taaaaactat gacagctttt
    33061 taccgcaaaa gttggatatt gtcgttttcc cgtcaaagta tggtggcgga gctggacacg
    33121 ttgaaattgt tgagagcgca aatttaaaca ctttcacatc atttggtcaa aactggaacg
    33181 gtaaaggttg gactaatggc gttgcgcaac ctggttgggg tcctgaaact gtgacaagac
    33241 atgttcatta ttatgacaat ccaatgtatt ttattaggtt aaacttccct aacaacttaa
    33301 gcgttggcaa taaagctaaa ggtattatta agcaagcgac tacaaaaaaa gaggcagtaa
    33361 ttaaacctaa aaaaattatg cttgtagccg gtcatggtta taacgatcct ggagcagtag
    33421 gaaacggaac aaacgaacgc gattttatac gtaaatatat aacgcctaat atcgctaagt
    33481 atttaagaca tgcaggacat gaagttgcat tatacggtgg ctcaagtcaa tcacaagata
    33541 tgtatcaaga tactgcatac ggtgttaatg taggcaataa aaaagattat ggcttatatt
    33601 gggttaaatc acaggggtat gacattgttc tagaaataca tttagacgca gcaggagaaa
    33661 gcgcaagtgg tgggcatgtt attatctcaa gtcaattcaa tgcagatact attgataaaa
    33721 gtatacaaga tgttattaaa aataatttag gacaaataag aggtgtaaca cctcgtaacg
    33781 atttactaaa tgttaatgta tcagcagaaa taaatatcaa ttatcgctta tctgaattag
    33841 gttttattac taataaaaat gatatggatt ggattaagaa aaattacgac ttgtactcta
    33901 aactaatagc tggtgcgatt catggtaagc caattggtgg agtggtagct agtgaggtca
    33961 aagcgccagt taaaaacgaa aagaatccgc cagtgccagc aggttataca ctcgataaga
    34021 ataatgtccc ttataaaaaa gaacaaggca attacacagt agctaatgtt aaaggtaata
    34081 atgtaagaga cggttattca actaattcaa gaattacagg ggtattaccc aacaacacaa
    34141 caattacgta tgacggtgca tattgtatta atggttatag atggattact tatattgcta
    34201 atagtggaca acgtcgctat attgcgaccg gagaggtaga catagcaggc aaccgaataa
    34261 gcagttttgg taagtttagt gcagtttgat aattgtatat gatgaatctt aggcaggtac
    34321 ttcggtactt gcctattatt taaaattaat aaacagttaa tttttacatg aatatattaa
    34381 attttaaaaa aacaaacgtt tttagtatat aaattatttt gtgttcgtat tgtgtgctat
    34441 gattaaaaag ttgttatggt caactatatc gtggttttat gtttattatc aatcaaaata
    34501 taaattattt ataatttgtt tggtaatgaa cgggtttttt tcgaaatgat agtaaaaaaa
    34561 cacatttgt
//
LOCUS       DAMZKC010000006        33842 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.006, whole genome shotgun sequence.
ACCESSION   DAMZKC010000006 DAMZKC010000000
VERSION     DAMZKC010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 33842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33842
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.006"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            257..1172
                     /locus_tag="POB55_000212"
                     /pseudo
     CDS             257..1172
                     /locus_tag="POB55_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000276756.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing protein"
     gene            1151..1891
                     /locus_tag="POB55_000213"
     CDS             1151..1891
                     /locus_tag="POB55_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDI6499210.1"
                     /translation="MAYTYTLKDIIEITGVTKRTLHYYDEIGLLVPDKNDKNYRVYKQ
                     QDLEKLQKILILKSFDFDIAKIKQYISYDNEQLRKLLSEQVSKLDKKISDLQLIRRSV
                     CEFINGHSLIDTSILNKTLQSQYDKEASIKYGHTKAYQSFIRRKDSLQSQDIRHKLTT
                     IFNKFNHMSLSHYPIQDCSDLVFEWKAFMNTIADFDDETLCCIAKTYEDDTRFKDYFN
                     SYDNQNLASYISEAVNYFLSNVNKSDNF"
     gene            complement(2149..2802)
                     /gene="nreC"
                     /locus_tag="POB55_000214"
     CDS             complement(2149..2802)
                     /gene="nreC"
                     /locus_tag="POB55_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002464689.1"
                     /note="Involved in the regulation of the the nitrate
                     reductase operon narGHJI; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate respiration regulation response
                     regulator NreC"
                     /protein_id="HDI6499211.1"
                     /translation="MKIVIADDHAVVRTGFSMILNYQNDMEVVATAADGVEAYQKVME
                     YKPDVLLMDLSMPPGESGLIATSKIADSFPETKILILTMFDDEEYLFHVLRNGAKGYI
                     LKNAPDEQLLLAIRTVYKGETYVDMKLTTSLVNEFVSNSNQDTANTTDPFKILSKREL
                     EILPLIAKGYGNKEIAEKLFVSVKTVEAHKTHIMTKLGLKSKPELVEYALKKKLLEF"
     gene            complement(2825..3859)
                     /gene="nreB"
                     /locus_tag="POB55_000215"
     CDS             complement(2825..3859)
                     /gene="nreB"
                     /locus_tag="POB55_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501138.1"
                     /note="Involved in the regulation of the the nitrate
                     reductase operon narGHJI; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate respiration regulation sensor histidine
                     kinase NreB"
                     /protein_id="HDI6499212.1"
                     /translation="MINEDSIQLDTLLKKYYEHSIEKIVFADDNGKIIAMNDAAKDIL
                     SEEDNYSAVANAICHRCEGYTNAYDVQSCKDCFLESMQVQATNFQVFMKTKDQKVMPF
                     TATYQLIDQDRGIHAFTLQNVSSQIEQQEKLHQQRMMRKTISAQENERKRISRELHDS
                     VIQEMLNVDVQLRLLKYQEDTTKLLEDAENIEYIVAKLIDDIRNMSVELRPASLDDLG
                     LEAAFKSYFKQFEENYGIKIIYTSNIKNTRFDSDIETVVYRVVQEAILNALKYADVNE
                     INVGIRQTGRHLVAEVIDAGNGFDPSSKPKGSGLGLYGMNERAELVSGSVNIETKIGE
                     GTNVTLNIPI"
     gene            complement(3883..4335)
                     /gene="nreA"
                     /locus_tag="POB55_000216"
     CDS             complement(3883..4335)
                     /gene="nreA"
                     /locus_tag="POB55_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate respiration regulation accessory nitrate
                     sensor NreA"
                     /protein_id="HDI6499213.1"
                     /translation="MTPEAIIEDRRFQETLDKIRKEEGYDFAAIAFYESNKPSSPIKW
                     HYVSGNKNNRFKLIILRKGRGLAGTVMKTGKRMVIANVGLALGPEEKIDYPILLSESL
                     TAVLAVPLWYKNQVYGVLLFGQRDGRPLPKIFDNDDIQRKFGIFNDDK"
     gene            complement(4355..5032)
                     /gene="narI"
                     /locus_tag="POB55_000217"
     CDS             complement(4355..5032)
                     /gene="narI"
                     /locus_tag="POB55_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HDI6499214.1"
                     /translation="MLNQFLWVIFPYLCLVVFVAGHIARYRYDKFSWTAKSSELIERK
                     RLMWGSLLFHLGIIPVFFGHVVGLLIPKSWMDAVGVSEHLYHIGAVYIGSIFGIITLI
                     GMFLLTARRVTTKSIRRLSSASDIFVNFLLLLIVFMGCYATLVTNIQHPDFNYRTSLS
                     IWFRQLFMLKPDASLMSGVPIAFKMHILLGFTIMACWPFTRLVHVWSVPLTYINRRYI
                     IYRKNKV"
     gene            complement(5025..5600)
                     /gene="narJ"
                     /locus_tag="POB55_000218"
     CDS             complement(5025..5600)
                     /gene="narJ"
                     /locus_tag="POB55_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HDI6499215.1"
                     /translation="MINFDNFKKYQESFGYMAQQLCFPEKLTFHPKTFEETISTDHPA
                     YEDLVAFRNVMMTFSLSEIKAIYTDTFDFTKKAPLYMTYNKFDTQKERGQMLAKLKVL
                     YEMFGLEMVDNELSDYLPLMLQFLQVAEWRNDPRAEENIQLIIMIIEDGTYVMANALG
                     EDNNPYAYVIQALRKTLKLCLTSPKGVKHHA"
     gene            complement(5593..7152)
                     /gene="narH"
                     /locus_tag="POB55_000219"
     CDS             complement(5593..7152)
                     /gene="narH"
                     /locus_tag="POB55_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009384084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HDI6499216.1"
                     /translation="MKIKAQVAMVLNLDKCIGCHTCSVTCKNTWTNRPGAEYMWFNNV
                     ETKPGVGYPKRWEDQEHYKGGWVLNRKGKLELKSGSRISKIALGKIFYNPDMPLIKDY
                     YEPWNYNYEHLTTAKSGKHSPVARAYSEITGDNIEIEWGPNWEDDLAGGHVTGPKDPN
                     IQKIEEDIKFQFDETFMMYLPRLCEHCLNPSCVASCPSGAMYKRDEDGIVLVDQDACR
                     GWRYCMTGCPYKKVYFNWKTNKAEKCTFCFPRIEAGMPTVCSETCTGRMRYLGVLLYD
                     ADRVHEAASAVDEKDLYEKQLDIFLNPFDEEVIAQAEKDGIGYDWIEAAQNSPIYKLA
                     IEYKLAFPLHPEFRTMPMVWYCPPLSPIMSYFEGKNTTQNPDAIFPAIEEMRLPIEYL
                     ANIFTAGDTEPVKGALQRMAMMRSYMRSQVTQQPFDTSRLERLGITERQTKDMYRLLG
                     LAKYEDRFVIPTSHKETYLDTYHAQGSTGYNYGGEHFGDNCEGCGVAVGSGKTGQEIY
                     NENFYGGIFRD"
     gene            complement(7142..10831)
                     /locus_tag="POB55_000220"
     CDS             complement(7142..10831)
                     /locus_tag="POB55_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HDI6499217.1"
                     /translation="MGKFGLNFFKPTEKFNGNWSILESKSREWEKMYRERWSHDKEVR
                     TTHGVNCTGSCSWKVFVKNGVITWENQQTDYPSCGPDMPEYEPRGCPRGASFSWYEYS
                     PLRIKYPYIRGKLWDLWTEALEENNGNRVAAWASIVENEDKAKQYKQARGMGGHVRSN
                     WKDVTEIIAAQLLYTIKKYGPDRIAGFTPIPAMSMISYAAGARFINLLGGEMLSFYDW
                     YADLPPASPQIWGEQTDVPESSDWYNASYIIMWGSNVPLTRTPDAHFMTEVRYKGTKV
                     ISVAPDYAENVKFADNWLAPNPGSDAAIAQGMTHVILQEHYVNQPNERFINYAKQYTD
                     MPFLIMLDEDENGYKAGRFLRASDLGQTTEQGEWKPVIHDAISDSLVVPNGTMGQRWE
                     EGKKWNLKLETEDGSKINPTLSMAEGGYELETIQFPYFDSDGDGIFNRPIPTRQVTLA
                     NGDKVRIATIFDLMASQYGVRRFDHKLESKGYDDAESKYTPAWQEAISGVKQSVVIQV
                     AKEFAQNAIDTEGRSMIIMGAGINHWFNSDTIYRSILNLVMLCGCQGVNGGGWAHYVG
                     QEKCRPIEGWSTVAFAKDWQGPPRLQNGTSWFYFATDQWKYEESNVDRLKSPLAKTEE
                     LKHQHPADYNVLAARLGWLPSYPQFNKNSLLFAEEAKDEGIDSNEAILKRAINEVKSK
                     QTQFAIEDPDLKKNHPKSLFIWRSNLISSSAKGQEYFMKHLLGTKSGLLATPNENEKP
                     EEITWREETTGKLDLVVSLDFRMTATPLYSDIVLPAATWYEKHDLSSTDMHPYVHPFN
                     PAIDPLWESRSDWDIYKTLAKAFSEMAKDYLPGTFKDVVTTPLSHDTKQEISTPYGVV
                     KDWSKGEIEAVPGRTMPNFAIVERDYTKIYDKYVTLGPVLEKGKVGAHGVSFGVSEQY
                     EELKSMLGTWSDTNDDSVRANRPRIDTARNVADAILSISSATNGKLSQKSYEDLEEQT
                     GMPLKDISSERAAEKISFLNITSQPREVIPTAVFPGSNKQGRRYSPFTTNIERLVPFR
                     TLTGRQSYYVDHEVFQQFGESLPVYKPTLPPMVFGNRDKKIKGGTDALVLRYLTPHGK
                     WNIHSMYQDNKHMLTLFRGGPTVWISNEDAEKHDIQDNDWLEVYNRNGVVTARAVISH
                     RMPKGTMFMYHAQDKHIQTPGSEITDTRGGSHNAPTRIHLKPTQLVGGYAQISYHFNY
                     YGPIGNQRDLYVAVRKMKEVNWLED"
     gene            complement(11139..12116)
                     /gene="cobA"
                     /locus_tag="POB55_000221"
     CDS             complement(11139..12116)
                     /gene="cobA"
                     /locus_tag="POB55_000221"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HDI6499218.1"
                     /translation="MSVEEYGKVYLIGAGPGNPNYLTKKAERLIREADVILYDRLVNP
                     LILQYANLTTEIIDVGKKPYAKHIQQEKINDCIVEAARRYNKVVRLKGGDPAIFGRVQ
                     EEVDTLNEHHIAFEIVPGVTSASAAVATMQTGLTMRTVAKSVTFSTGHFKDSEENEVD
                     VNSLVNGGTLAIYMGVKRLGKIITQIQQYTDIDYPIAIVFQASCFNEFVVKGRLSNII
                     SKLQHYSIEAKPGICIIGEVVGYTENVSTTSNPTQQFYVVSGSKHDALMLCEHLYDEG
                     YGCLLNPNNTSNGTYHSSQYDYYDAFIKQQENVTYISTDRADANTVLCH"
     gene            complement(12107..12421)
                     /gene="nirD"
                     /locus_tag="POB55_000222"
     CDS             complement(12107..12421)
                     /gene="nirD"
                     /locus_tag="POB55_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HDI6499219.1"
                     /translation="METKEKIKVTTIDELTPLIGKKVIVKDKEVGLFLTESGKIHAIH
                     NICPHKQGPLSEGTVSGEYVFCPLHDQKIDLNTGIVQEPDEGCVDVYEVEVTDGNVYI
                     CL"
     gene            complement(12425..14830)
                     /locus_tag="POB55_000223"
     CDS             complement(12425..14830)
                     /locus_tag="POB55_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDI6499220.1"
                     /translation="MAKQKLVMIGNGMAGIRTIEEILERANDLYDITVIGKEPYPNYN
                     RIMLSNILQNKMTVEETIMNPYEWYEEHGIELITNDPVIEVDRANQSVTTANGIEVSY
                     DKLIFATGSKAFVIPVPGSTLPSVIGWRTIDDTEQMMNIAKTKKKAIVIGGGLLGLEC
                     ARGLLDQGMEVTVLHLAEWLMEMQLDRKAGNMLKADLEKQGMKFEMQANTTEILGEDD
                     VEGVKLADGREIPADLVVMAVGIRPYTEVAKESGLDVNRGIVVNDVMQTSDSNVYAVG
                     ECAEHNGKVYGLVAPLYEQGKVLADHLTNKETNGYKGSTTFTSLKVSGCDLYSAGQIV
                     ENAEIKGIEIFNSVDNNYKKIFLKDGNVVGAVLYGDIDDGSRFYNMMKKGESTEDYTL
                     VSLLTKGGEEASLSIADMADDETICGCNGVDKGTIVNAITENGFTTVEEVTAKTKAGN
                     SCGKCKPQIAQILQHTLGDDFVAAKPAGICGCTDLTRDQIVTQIRAKGLKTSKEVRHV
                     LNFKNKGGCPKCRPAINYYLNMVYPHDHEDERESRFANERYHANIQNDGTFSVIPQMR
                     GGVTDADQLIRLGEVAKKYHVPLVKVTGSQRVGLYGVKKEELPNIWEDLGMRSASAYG
                     KKTRSVKSCVGKEFCRFGTQYTTRLGIRLEKTFEYIDTPHKFKMGVSGCPRSCVESGV
                     KDFGIISVENGFQIYIGGNGGTEVEKAEFLTTVETEDEVIKLCGALMQYYRETGIYAE
                     RTAPWLRRLGFENVKEVLLDPERQNELFERIMDAKKAVEAEPWEAITSNAQARKIFEV
                     EKV"
     gene            complement(14896..15627)
                     /locus_tag="POB55_000224"
     CDS             complement(14896..15627)
                     /locus_tag="POB55_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin chelatase"
                     /protein_id="HDI6499221.1"
                     /translation="MNGNIIVAHGMRHGRQNQALEAFISELVKDDIHHYDIAFLESEH
                     QDLETVMTTLIQSGVDHFKIVPLLIFSAMHYLKDIPNIVHEMKRRYPDIKVEVSEPLG
                     THPLMRRLIEQRICDALAGEEQQVGVMVVAHGNINGKFTKAHEELQICTQNLQISKPT
                     YARTLYGEISFTHDLESISKRYQKLIVVPLFLYDGRLVNKVKQQMNDMVINTDIHITP
                     SINFDPILKQIINDRLESLMIPMKI"
     gene            complement(15856..16383)
                     /locus_tag="POB55_000225"
     CDS             complement(15856..16383)
                     /locus_tag="POB55_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6499222.1"
                     /translation="MMIRLASEQDAEKLQQLMHEAFTPLRELGIDWPSVNANLDAVKE
                     NIDKNTTFVMTIDDEIISTITVRYPWGSVKSISGYPFVWWFATNPEYEGKGYGSQLLT
                     YVEEAFLRDTLKSAAVTLGTSARLHPWLLKIYEKRGYEIYSEHENDDGDLGVIMRKVL
                     IPERFDETILGQPPF"
     gene            complement(16627..17451)
                     /locus_tag="POB55_000226"
     CDS             complement(16627..17451)
                     /locus_tag="POB55_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDI6499223.1"
                     /translation="MIENKKTVEDTYSTGAIVDSISSSVQMKQVMVQQTPGRYMLKAM
                     MAGFLLSIVTVFMFGIKTQFASTHVDGLINLMGAIAFSLGLILVVLTNSELLTSNFMY
                     FTVGWYYKVVSVKKMTWILLYCFLGNILGGFVLFFLMKFAHVMTPEMTQALTALVQKK
                     TVDSTWLNIFTKGIFCNFFINIGIFISMQFKGGLTKAFFIACGVVVFVYMGYEHVVFN
                     AGLYAGMVFFNLDAVSWLHVLKNIVFAFLGNFVGGGIFVGLVYAFLNGKRNRLEQQ"
     gene            complement(17668..17850)
                     /locus_tag="POB55_000227"
     CDS             complement(17668..17850)
                     /locus_tag="POB55_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499224.1"
                     /translation="MVERYIKVLILYIFTTLLSSISVTSKCVPNKVIRFILRTAIGYS
                     VFAYGLHYFSNLKKNK"
     gene            complement(17934..18401)
                     /locus_tag="POB55_000228"
     CDS             complement(17934..18401)
                     /locus_tag="POB55_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HDI6499225.1"
                     /translation="MTIKVEDNKIIFSRTIEAPIEKVFDAYTTKALFEKWFHPKDAST
                     KVFRFNAVSGGDAFYAIKTPTMTSYTLAEYKTVKRPYLIEYIDSFATPQGAKDTKMPS
                     MKITLSFSKSNTTKTTVTSTSVFPTKEAAQQVINMGVEQGMNQTLDQLDALLK"
     gene            complement(18587..20137)
                     /gene="adcA"
                     /locus_tag="POB55_000229"
     CDS             complement(18587..20137)
                     /gene="adcA"
                     /locus_tag="POB55_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HDI6499226.1"
                     /translation="MKKKLGMLLLVPAVTLSLAACGNDDGKDKDGKVTIKTTVYPLQS
                     FAEQIGGKHVKVSSIYPAGTDLHSYEPTQKDILSASKSDLFMYTGDNLDPVAKKVAST
                     IKDKDKKLSLEDKLDKAKLLTDQHEHGEEHEHEGHDHEKEEHHHHHGGYDPHVWLDPK
                     INQTFAKEIKDELVKKDPKHKDDYEKNYKKLNDDLKKIDNDMKQVTKDKQGNAVFISH
                     ESIGYLADRYGFVQKGIQNMNAEDPSQKELTKIVKEIRDSNAKYILYEDNVANKVTET
                     IRKETDAKPLKFYNMESLNKEQQKKDNITYQSLMKSNIENIGKALDSGVKVKDDKAES
                     KHDKAISDGYFKDEQVKDRELSDYAGEWQSVYPYLKDGTLDEVMEHKAENDPKKSAKD
                     LKAYYDKGYKTDITNIDIKGNEITFTKDGKKHTGKYEYNGKKTLKYPKGNRGVRFMFK
                     LVDGNDKDLPKFIQFSDHNIAPKKAEHFHIFMGNDNDALLKEMDNWPTYYPSKLNKDQ
                     IKEEMLAH"
     gene            complement(20431..20697)
                     /locus_tag="POB55_000230"
     CDS             complement(20431..20697)
                     /locus_tag="POB55_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HDI6499227.1"
                     /translation="MARLNITFSPQAFEDYKYFQQNDKKMVKKINELLKSIDRNGALE
                     GIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIKIASCKYHY"
     gene            complement(20697..20948)
                     /locus_tag="POB55_000231"
     CDS             complement(20697..20948)
                     /locus_tag="POB55_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDI6499228.1"
                     /translation="MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESD
                     YNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKDIDV"
     gene            complement(21113..21214)
                     /locus_tag="POB55_000232"
                     /pseudo
     CDS             complement(21113..21214)
                     /locus_tag="POB55_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501155.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21221..21820)
                     /locus_tag="POB55_000233"
     CDS             complement(21221..21820)
                     /locus_tag="POB55_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDI6499229.1"
                     /translation="MTKKLLTLFIVSMLILTACGKKESATTSSKNGKPLVVIYGDYKC
                     PYCKELDEKVMPKLRKNYIDNHKVEYQFVNLAFLGKDSIVGSRASHAVLMYAPKSFLD
                     FQKQLFAAQQDENKEWLTKELLDKHIKQLHLDKETENKIIKDYKTKDSKSWKAAEKDK
                     KIAKDNHIKTTPTAFINGEKVEDPYDYESYEKLLKDKIK"
     gene            complement(21839..22201)
                     /locus_tag="POB55_000234"
     CDS             complement(21839..22201)
                     /locus_tag="POB55_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4467 domain-containing protein"
                     /protein_id="HDI6499230.1"
                     /translation="MKRFVATVLLLLVFISGCGNDKYVKEIDEAVKIQNQKQEQLAKK
                     GNGDRVDHFERKDANIYVYDKDKIIILAYKPLSNDDEVHYYAYDFSDKRVSYKQDFDS
                     RRYYQQHDADYHEENMTN"
     gene            22456..23706
                     /gene="fmhA"
                     /locus_tag="POB55_000235"
     CDS             22456..23706
                     /gene="fmhA"
                     /locus_tag="POB55_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FemA/FemB family glycyltransferase FmhA"
                     /protein_id="HDI6499231.1"
                     /translation="MNFVTLTSDEFNAFTTKHFSHYTQSTIHYNHRVDLKGDVHLVGV
                     KDDNGQVIAGCLLTEARTLKFFKYFYTHRGPVMDYTNQSLVAFFFKALTSYLKKHNCL
                     YVLVDPYLIENLRNADGEIVKSYDNRAFVRTMDKLGYKHQGFPVGYDSMSQIRWLSVL
                     DLKDKTEDQLLKEMDYQTRRNIKKTYDIGVKTKTLTIDETQTFFDLFHMAEEKHGFKF
                     RELPYFEEMQKLYDDHAMLKLAYIDLNEYLKTLQLKQQQLTAELSGVEEALEESPNSK
                     KNKTKRTQLEQQLNSNKRKIDNTIEQIEQDGAVLNLASALFIYNEHEVYYLSSGSNPK
                     YNAYMGAYHLQWEMIKFAKAHHIDRYNFYGITGDFSESSEDYGVQQFKKGFNAHVEEY
                     IGDFIKPIKPLLYKVQTYLNHKRR"
     gene            complement(23803..24534)
                     /locus_tag="POB55_000236"
     CDS             complement(23803..24534)
                     /locus_tag="POB55_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDI6499232.1"
                     /translation="MIQLNNIHKSFNDVEVIKGIDLSVEQGEVVTLIGRSGSGKTTLL
                     RMINALEIPTEGTVYVNGKTYTSKDKKSQIEVRKQSGMVFQSYNLFPHKTALENVMEG
                     LITVKKLKKDEARGKSLELLEKVGLTHVKDQRPHALSGGQQQRVAIARALAMNPKVML
                     FDEPTSALDPELVNDVLKVIKDLANEGMTMVIVTHEMRFAKEVSNNIVFIHEGMIGEQ
                     EAPEEMFNRPKTEELRRFLNVINEE"
     gene            complement(24531..25250)
                     /locus_tag="POB55_000237"
     CDS             complement(24531..25250)
                     /locus_tag="POB55_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDI6499233.1"
                     /translation="MFLNLNSEQQHALDAAKQAFGPMLEGLVKYSIPITLVTFVLGLI
                     IALFTALMRISTSKILRSISRVYVSIIRGTPMIVQLFIIFYGIPELGRLLTNDADNQW
                     TLAPVVAAIIGLSLNVGAYASEIIRGGIISIPKGQTEAAYSIGMTYGQTIQRIILPQA
                     IRVSIPALGNTFLSLIKDTSLLGFILVAEMFRKAQEVASTTYEYLTIYVLVALMYWVV
                     CFIISIIQGIYESYIERGYRS"
     gene            complement(25231..26010)
                     /locus_tag="POB55_000238"
     CDS             complement(25231..26010)
                     /locus_tag="POB55_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDI6499234.1"
                     /translation="MKRLLFVMIAFVFILAACGNNSSKDKEASKDSKTINVGTEGTYA
                     PFSFHDKDGKLTGYDIDVIKAVAKEEGLKLKFNETSWDSMFAGLDAGRFDVIANQVGI
                     NPDREKKYKFSKPYTFSSAVLVIRENEKDIKDFDDVKGKKLAQTFTSNYGKLAKDKGA
                     DITKVDGFNQSMDLLLSKRVDGTFNDSLSYLDYKKQKPNAKIKAIKGNAEQSRSAFAF
                     SKKADDETVQKFNDGLKKIEENGELAKIGKKWFGQDVSKSK"
     gene            complement(26131..27570)
                     /gene="mdeA"
                     /locus_tag="POB55_000239"
     CDS             complement(26131..27570)
                     /gene="mdeA"
                     /locus_tag="POB55_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdeA"
                     /protein_id="HDI6499235.1"
                     /translation="MSKKQKLTMIITMLMGGFFGLLNETLLVTALPSIMKDFEISYTQ
                     VQWLTTAFLLTNGIVIPLSALVIQRYTTRQVFLVGISIFFLGTLLGGLSPHFATLLVA
                     RIIQALGAGIMMPLMMTTILDVFQPHERGKYMGIFGLVIGLAPAIGPTLSGYLVEYFN
                     WRSLFHVVAPIAAVTFLIGFKTIKNVGTTIKVPIDFISVIFSVLGFGGLLYGTSSISE
                     KGFDNPIVLLSMIGGVVLVALFVLRQYRLSTPLLNFAVFKNKQFTVGIIIMGVTMVSM
                     IGSETILPIFVQNLLHRSALDSGLTLLPGAIVMAFMSMTSGALYEKFGPRKLALVGMA
                     IVVITTAYFVVMDEQTSTIMLATVYAIRMVGIALGLIPVMTHTMNQLKPEMNAHGSSM
                     TNTVQQIAGSIGTAALITILSHASKNFSPTMSDYNGMNKIDMMNQIKVDTMLHGYHAG
                     FLFALLITVISFFCSFMLQGKKKEVDSRQ"
     gene            27744..27848
                     /locus_tag="POB55_000240"
     CDS             27744..27848
                     /locus_tag="POB55_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HDI6499236.1"
                     /translation="MKLDLQTARRNLNSPNIKTRKRALKIIKQHKRAK"
     gene            complement(28002..28688)
                     /locus_tag="POB55_000241"
     CDS             complement(28002..28688)
                     /locus_tag="POB55_000241"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDI6499237.1"
                     /translation="MPKLILCRHGQSEWNAKNLFTGWEDVNLSEQGINEATRAGEKVR
                     ENNIAIDVAFTSLLTRALDTTHYILTESKQQWIPVYKSWRLNERHYGGLQGLNKDDAR
                     KEFGEEQVHIWRRSYDVKPPAETEEQREAYLADRRYNHLDKRMMPYSESLKDTLVRVI
                     PFWTDHISQYLLDGQTVLVSAHGNSIRALIKYLEDVSDEDIINYEIKTGAPLVYELTD
                     DLEVIDKYYL"
     gene            complement(29015..29881)
                     /locus_tag="POB55_000242"
     CDS             complement(29015..29881)
                     /locus_tag="POB55_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDI6499238.1"
                     /translation="MSHNENLKLAQRGAYLSLIVYIILSIVKYVTGFVFNSAAVRADA
                     LNNMTDIIVSLAVIIGLKISIKPADRNHPYGHLKSENISSLLVSFVIMFVGIQVVIQN
                     APRLFKEDDVVPNAITIIVSLISGLVMLIVFAVNQRLAKRTKSSSLNSAAKDNLSDSL
                     VSIGTAIGLIFTQIGFPIVDIILATLLGLLIVYTGFGIFKEAIFMLSDGFNETELEAY
                     RNDILEVDEVQEVKSIKGRYHGSSVFIDVTIVVDANLSLVEAHQICDNVEHHLHKKGI
                     SSVYVHPEPDHL"
     gene            complement(30380..30484)
                     /locus_tag="POB55_000243"
     CDS             complement(30380..30484)
                     /locus_tag="POB55_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499239.1"
                     /translation="MLTNRLTSVNYVKKRLIQNIQRILSKEVLYGEMQ"
     gene            30579..31892
                     /gene="sbi"
                     /locus_tag="POB55_000244"
     CDS             30579..31892
                     /gene="sbi"
                     /locus_tag="POB55_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin-binding protein Sbi"
                     /protein_id="HDI6499240.1"
                     /translation="MKNKYISKLLVGAATITLATMISNGEAKASENTQQTSTKHQTTQ
                     NNYVTDQQKAFYQVLHLKGITEEQRNQYIKTLREHPERAQEVFSESLKDSKNPDRRVA
                     QQNAFYNVLKNDNLTEQEKNNYIAQIKENPDRSQQVWVESVQSSKAKERQNIENADKA
                     IKDFQDNKAPHDKSAAYEANSKLPKDLRDKNNRFVEKVSIEKAIVRHDERVKSANDAI
                     SKLNVKDSIENRRLAQREVNKAPMDVKEHLQKQLDALVAQKDAEKKVAPKVEAPQIQS
                     PQIEKPKAESPKVEVPQIQSPKVEVPQSKLLGYYQSLKDSFNYGYKYLTDTYKSYKEK
                     YDTAKYYYNTYYKYKGAIDKAVLTLLGDGSKSYIQPLKVDDKNGYLAKSYAQVRNYVT
                     ESINTGKVLYTFYQNPTLVKTAIKAQETASSIKNTITGLFNSFWK"
     gene            32428..33357
                     /gene="hlgA"
                     /locus_tag="POB55_000245"
     CDS             32428..33357
                     /gene="hlgA"
                     /locus_tag="POB55_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component gamma-hemolysin HlgAB subunit A"
                     /protein_id="HDI6499241.1"
                     /translation="MIKNKILTATLAVGLIAPLANPFIEISKAENKIEDIGQGAEIIK
                     RTQDITSKRLAITQNIQFDFVKDKKYNKDALVVKMQGFISSRTTYSDLKKYPYIKRMI
                     WPFQYNISLKTKDSNVDLINYLPKNKIDSADVSQKLGYNIGGNFQSAPSIGGSGSFNY
                     SKTISYNQKNYVTEVESQNSKGVKWGVKANSFVTPNGQVSAYDQYLFAQDPTGPAARD
                     YFVPDNQLPPLIQSGFNPSFITTLSHERGKGDKSEFEITYGRNMDATYAYVTRHRLAV
                     DRKHDAFKNRNVTVKYEVNWKTHEVKIKSITPK"
BASE COUNT        10981 a         6469 c         4771 g        11621 t
ORIGIN      
        1 atagacaaag tattgactac atatatattt tattatgatt gtgaaaaagt gaacataagg
       61 attttccctt atttatgaag tgatttaccc tatacaatgt ccctttaaga tgcttttaga
      121 cacttattcg ccatcatttt aatcatatta tttaggaaca ctcacatact tttcttattt
      181 ttttcaatta gttacaatta ctgcttgaaa atcacgttac gttatccttt atgctgacaa
      241 cataaggagg tttatcatgt ataaaatcaa tattattaga tcagattccg tctataagaa
      301 tatactcaag gcaccaataa acgaccgtga ttcaattttc actaaagaaa tattagtacc
      361 ttttaaaaag aaatttgagg tacaacatat gcctatttat aatgatgaca aacaaacaat
      421 gagtgcgatt caatttttag atgcatttca aaagtctccc aaagaactgg ttgaaactga
      481 ccaaatgtca atacaatacc tgaataatga tttctggagt aattgcgaaa aatatttaaa
      541 agttgctata gatcaatttt caaattatag tatctcattt caagtttcta actatcattt
      601 cacagtatta cttggagata gccaaaaacc acttatgtat ctaaataaaa atcgcggtgg
      661 tgatggtggc ataccaggtt atattatgat ttatttagtg ccgagtacaa gtacaattaa
      721 ttctatgaaa agcttaattg cacatgaagt aaatcataat atgcgctatc aatattattg
      781 attgggatgg cggaagtttg attgaattga ttattgcaga aggattagct gaaaactatg
      841 tagaatcatt gtatggtaaa gcgcatattg gaccgtgggt aacaaatact aattggagtc
      901 gtgataacgt aaaaattaaa aatactattt ataatcattt acatttaaag catatatttg
      961 aatcgatgcc ttatctctat ggtgatgata ttaataaact tcaaggtagg cctatcgttg
     1021 gcttatctca tgctgccggg tatgcatgtg gctatcactt ggtaaaatac tttttacaaa
     1081 aaacaaacat acctattgaa gttgccacaa cacttccagc acaaaaaata ataaatgagg
     1141 tgactgaatt ttggcataca catacacttt aaaagatatt attgaaatta caggtgtaac
     1201 taaaagaact ttacattatt acgatgaaat aggattatta gttccagata aaaatgataa
     1261 aaattatcgc gtttataaac agcaagactt agaaaaatta caaaagattt taatactcaa
     1321 gtcttttgat tttgatatcg ctaaaataaa acaatacatt tcatatgata atgaacaatt
     1381 gcgaaagtta ttatcagagc aagtaagcaa gttagataaa aagatttcag acttacaatt
     1441 aattagacgc tctgtatgtg aatttattaa cggacactct ctaatagata ccagcatttt
     1501 aaataagaca ctacagtcac aatatgataa agaagcatct ataaaatatg gtcatacgaa
     1561 agcatatcaa tcatttatta gacgtaaaga cagcttacaa tcgcaggata tcagacataa
     1621 attgacaact atcttcaata aatttaatca tatgtctttg agtcattatc caatccaaga
     1681 ttgtagtgat ctcgtatttg agtggaaggc atttatgaac actatcgctg attttgatga
     1741 tgaaacatta tgctgtattg ctaaaacata tgaagatgat acgcgtttca aagattactt
     1801 taattcatat gataatcaaa atttagcatc atacatttca gaagctgtta attatttttt
     1861 gagcaatgtg aataagagcg acaattttta atcctcatac agatgctaca acactgaata
     1921 caccaactaa aataattgtt caaagtagaa aactaatcat ttattctcta aatcaataac
     1981 tatattaaaa gttatacctt tgcaaagcga attagtaaag gttaccgaaa ggagaaagga
     2041 ttaggttcca ttcgatttat aaaaaaatat atttcgaaac atacaaaagc gccagaagat
     2101 aatcatttcc tctcgcgctt ttatactgaa ttatattaat ttcaaactct aaaactctaa
     2161 taatttcttt ttcaatgcat attcaactag ctcaggttta ctctttaagc caagctttgt
     2221 cataatatgc gtcttatgtg cttctactgt tttcacagat acaaataatt tctctgcaat
     2281 ttctttattc ccgtaacctt tggcaataag tggcaatatt tctagttctc gttttgataa
     2341 gattttaaaa ggatctgttg tgtttgcagt gtcctgattt gaattagata caaattcatt
     2401 cactaaagat gttgtaagtt tcatatctac atatgtttca cctttatata cagttcgaat
     2461 agctaacaat aattgttcat caggtgcatt tttcaatatg taacctttcg caccattacg
     2521 caacacatgg aacaaatact cctcatcatc aaacatggtt aatattaaaa ttttagtttc
     2581 tggaaaactg tcagcaattt tactcgtagc gataagacct gactcacctg gtggcatact
     2641 taaatccatt agtaacacat caggtttata ttccattact ttttggtaag cttcgacgcc
     2701 atctgcagcc gttgcaacaa cttccatatc attttgataa tttaaaatca tagagaaccc
     2761 cgtacggaca acagcgtgat catcggcaat gactattttc aattttattc ccccaatgta
     2821 tgtttcaaat tggaatgttc aatgtaacat tggtaccctc accaattttc gtttcaatat
     2881 tgacgctacc gctgactaac tcagctcgct cattcattcc atataaaccg agtccagaac
     2941 ctttaggctt agaacttgga tcaaaaccat ttcccgcatc tatcacttct gctaccaaat
     3001 ggcgcccagt ttgacggata cctacattta tttcatttac atcagcgtat ttcaacgcat
     3061 ttagaatagc ttcttgtact actcgataaa caaccgtttc aatatcacta tcaaagcggg
     3121 tatttttaat atttgatgta tatatgattt ttattccata attttcttca aactgtttaa
     3181 aatatgattt aaaagctgct tcaaggccta gatcatccaa agaagcgggt cttaattcaa
     3241 ccgacatatt acgtatatca tcaattaatt tagcgacaat atattcaata ttttctgcgt
     3301 cttccaaaag cttagttgta tcttcttgat attttaataa tctcaattga acatctacat
     3361 tgagcatttc ttgaatcaca ctatcatgta actctctaga aattcgcttt ctttcatttt
     3421 cttgggctga gattgtttta cgcatcatac gttgttgatg taatttctct tgctgttcaa
     3481 tttgtgatga aacattttga agcgtaaatg catgaattcc cctgtcttga tcaatcaact
     3541 gatatgttgc tgtaaatggc atcacttttt gatctttcgt cttcataaat acttggaaat
     3601 tcgtagcttg tacttgcatc gattctaaga aacagtcttt acatgattgt acatcatagg
     3661 catttgtata cccttcacat ctgtgacaaa tcgcattcgc cacagcactg taattatctt
     3721 cttcagataa tatatcttta gcagcatcat tcattgcaat aattttgccg ttatcatcag
     3781 caaaaactat cttttcgatt gaatgctcat aatatttttt caataaagta tctaactgta
     3841 tactgtcctc attaatcatg acttacaccc taattcatct cattatttat catcattgaa
     3901 aataccaaac ttacgttgaa tatcatcatt atcaaatatt tttggtaaag gacgaccatc
     3961 tctttgacca aataatagta cgccatacac ttgattctta taccaaagcg gcactgctaa
     4021 aactgctgtt aatgattcgc tcaataaaat tggatagtca atcttttctt caggccctaa
     4081 agctaaacca acattggcta ttaccatacg ctttcctgtt ttcataacag ttccagctaa
     4141 tccacgacct tttcttaaaa taatcaattt aaatcgatta tttttattac ctgaaacata
     4201 gtgccatttt attggagatg atggtttgtt agattcatag aaagcgattg ccgcaaaatc
     4261 ataaccctct tctttgcgta ttttatctaa tgtctcttga aatctacgat cttcaattat
     4321 tgcttctggt gtcaaatcct ttcacctctt atgcttacac tttattctta cggtaaataa
     4381 tatatctgcg atttatatat gtcaaaggta cactccaaac atgcaccaaa cgtgtaaatg
     4441 gccaacaagc cataatagtg aaacctaaca atatatgcat tttaaatgca atcggcacac
     4501 cactcatcaa tgacgcatct ggttttaaca taaataattg tctaaaccaa attgataatg
     4561 aagttctgta gttaaaatct ggatgttgta tattggttac taatgttgcg taacatccca
     4621 taaatacgat aagtaataat aagaaattta caaatatatc cgacgctgaa cttaatcttc
     4681 gaatactttt cgtagtaaca cgtctcgctg ttaataaaaa catccctatc aaagttatta
     4741 taccaaagat gctaccaata taaacagcgc ctatatgata taaatgctca gacacaccca
     4801 ctgcatccat ccatgatttc ggtattaaca atccaactac gtgtccaaaa aacactggaa
     4861 taatacctaa gtgaaataat aaacttcccc acatcaatct ttttctttct attagttcac
     4921 tagatttagc tgtccaagaa aatttatcat aacgataacg tgcaatatga cctgcgacaa
     4981 agacaactaa acataaatac ggaaatataa cccataaaaa ctgattaagc atgatgtttc
     5041 actccttttg gtgatgtcaa acataatttc aatgtttttc taagtgcttg aatcacatag
     5101 gcatatggat tgttatcttc accaagtgca ttcgccatca catatgttcc atcctcaata
     5161 atcataatga ttaattgaat attctcttca gctcttggat catttcgcca ttctgccact
     5221 tgcaaaaatt gaagcatcaa cggtagataa tcagaaagtt cattatctac catttctagt
     5281 ccaaacattt catataatac ctttaattta gctaacattt gcccacgttc tttttgcgta
     5341 tcaaatttgt tatacgtcat atataatggt gcttttttcg taaaatcaaa tgtatctgta
     5401 taaatcgctt tgatttctga taatgaaaat gtcatcatta catttctaaa tgctactaaa
     5461 tcttcatatg ctggatgatc tgtcgaaatt gtttcttcaa aagtttttgg atgaaaagtt
     5521 aatttttctg gaaagcataa ctgttgtgcc atatatccaa aactttcttg atatttttta
     5581 aaattatcga aattaatcac ggaaaatccc tccatagaaa ttctcattat aaatttcttg
     5641 accagttttc cctgaaccta ctgcaacgcc acagccttca cagttatctc caaaatgctc
     5701 gccgccgtaa ttgtatcctg tactaccttg tgcgtgatac gtatctaaat aggtttcttt
     5761 gtgtgatgtt ggaataacaa atcgatcttc atatttggct agtcctaata aacgatacat
     5821 gtctttagtt tggcgctcgg ttatacctaa tcgctctaat cgagacgtgt caaatggctg
     5881 ttgagtaact tgagatctca tataacttct catcattgcc atacgttgta gggctccttt
     5941 tactggctct gtatctcctg cagtgaaaat attagctaag tattcaatag gtaaacgcat
     6001 ttcttcaatg gctgggaaaa tcgcatctgg attttgagtt gtatttttac cttcaaaata
     6061 gctcataatt gggctaagtg gtgggcaata ccaaaccatc ggcatcgttc taaattcagg
     6121 atgtaacgga aatgcaagtt tatattcaat tgctaactta taaattggag agttttgtgc
     6181 agcttcaatc caatcataac caataccatc tttttcagct tgagcaatga cttcttcgtc
     6241 aaatgggttt aagaatatat ctaattgttt ttcgtataaa tctttctcgt ctactgctga
     6301 agccgcttca tgaactcgat ctgcatcata taataaaaca cctaagtaac gcatacgtcc
     6361 tgtacaagtt tcagagcata ccgtaggcat acccgcctcg attctcggga aacagaaagt
     6421 acatttttca gctttgttcg ttttccaatt gaagtaaact ttcttatatg gacaacctgt
     6481 catacagtaa cgccatccac gacatgcgtc ttggtcaact aatacaatgc catcttcatc
     6541 acgtttatac atagcacctg aaggacacga tgcaacgcaa cttggattca agcaatgttc
     6601 acataaacgt ggtaaataca tcataaaagt ttcgtcaaat tggaatttaa tatcttcttc
     6661 tattttttgg atgttaggat cttttggacc tgtaacatga ccacctgcta agtcatcttc
     6721 ccagttaggt ccccattcaa tttcaatgtt atcccctgta atttctgaat acgctctagc
     6781 aactggcgaa tgcttccctg atttcgcagt tgttaaatgt tcataattat agttccatgg
     6841 ctcataataa tctttaatta atggcatatc tgggttataa aaaattttac ctaaagcaat
     6901 ttttgaaatt ctacttccag attttaattc aagtttccct ttacgattta gtacccaacc
     6961 acctttgtag tgttcttggt cttcccaacg tttcggatac cctacacctg gcttcgtttc
     7021 tacgttgttg aaccacatgt actcagcacc tggacgattt gtccaagtgt ttttacatgt
     7081 cacactacac gtatggcatc ctatgcattt atctaaattt aataccatcg caacttgcgc
     7141 tttaatcttc aagccaatta acctccttca tctttctaac tgctacatat aaatcccttt
     7201 ggttcccaat tggtccataa taattaaagt gataactaat ttgtgcgtat cctccgacta
     7261 gttgtgttgg tttcaaatgg attctagtcg gcgcgttgtg tgaaccacca cgtgtatctg
     7321 taatttctga cccaggcgtt tgaatatgtt tatcttgtgc atgatacata aacattgtac
     7381 ctttaggcat acgatgcgaa ataactgctc ttgccgttac aacaccatta cggttataca
     7441 cttctagcca atcattatct tggatatcgt gtttttcagc atcttcattt gatatccaaa
     7501 ccgttggacc acctctaaat agtgtcaaca tatgcttatt atcttgatac attgagtgta
     7561 tattccattt tccatgaggc gttaaataac gcagtaccaa agcatctgta ccacctttaa
     7621 ttttcttatc tctattccca aataccattg gcggcaatgt cggtttatat actggtaagc
     7681 tctctccaaa ttgttggaaa acttcgtgat ccacataata actttgacgt cctgttaatg
     7741 ttctaaaagg tactagacgt tctatattcg ttgtaaatgg tgaatatcgt cgaccttgtt
     7801 tatttgaacc tgggaatact gctgtcggta ttacttctcg tggttgtgaa gttatattta
     7861 aaaacgaaat tttctcagca gcgcgttcgc tagaaatatc ttttaacggc attccagttt
     7921 gttcttcgag atcttcatat gatttttgtg ataatttacc attcgtagca gatgaaatac
     7981 ttagtattgc atcagctaca ttacgtgctg tatcaatacg tggacgattc gctctcacag
     8041 aatcatcatt tgtatcactc cacgtaccta acatactttt taattcttca tattgttcac
     8101 tgacaccgaa acttacacca tgtgctccaa ctttcccttt ttcaagtaca ggaccaagcg
     8161 tgacatattt gtcgtaaatt ttagtgtagt cgcgttctac aattgcaaag ttaggcattg
     8221 tacgtccagg taccgcttca atttcaccct tcgaccaatc tttcactacg ccgtatggtg
     8281 ttgaaatttc ttgctttgta tcatgactaa gcggagttgt cacaacatct ttaaacgttc
     8341 caggtaaata gtcttttgcc atttctgaaa atgcttttgc caacgtttta taaatatccc
     8401 agtctgaacg cgattcccat aatggatcaa tagctggatt aaaaggatgt acatatggat
     8461 gcatatctgt agatgacaaa tcatgcttct cataccaagt cgctgctggc aaaacaatgt
     8521 cagaatataa aggtgttgct gtcattctga aatctaaaga aacgactaaa tctaatttcc
     8581 ctgttgtttc ctcacgccac gtaatttctt ctggcttttc attttcattt ggtgtagcta
     8641 ataaccctga ttttgtgcca agtaaatgct tcataaagta ttcttgacct tttgcagaac
     8701 ttgagattag atttgagcgc catataaaca gtgatttcgg atgattcttt ttcaaatccg
     8761 gatcttctat cgcaaattgc gtttgttttg acttaacttc atttatcgct cgttttaaaa
     8821 ttgcttcatt tgaatctata ccttcatctt tagcttcttc tgcaaacaac aaactatttt
     8881 tattaaattg tggatatgat ggtaaccaac caagtctagc tgctaaaaca ttataatcag
     8941 ctggatgttg atgctttaac tcctctgttt tagctaatgg agattttaaa cgatctacat
     9001 ttgactcttc atatttccat tggtctgttg caaaatagaa ccaacttgtt ccgttttgca
     9061 aacgtggtgg tccttgccag tctttcgcaa atgcgacagt actccatcct tcaatcggac
     9121 gacatttttc ttgtcccaca tagtgagccc aaccgccacc attcacacct tgacagccac
     9181 ataacataac taagtttaag attgaacgat aaatcgtatc tgagttaaac caatggttaa
     9241 tacccgcacc catgataatc attgaacgcc cttcagtatc gatagcgttt tgcgcaaatt
     9301 ctttcgctac ttgaatgaca acactttgtt ttacgcctga aatggcttct tgccaagcag
     9361 gtgtatattt tgattctgca tcgtcgtatc cttttgattc taatttatga tcaaaacgac
     9421 gcacgccata ttgacttgcc attaagtcaa aaattgtagc aatacggact ttgtcaccat
     9481 ttgctaaagt gacttgtcga gttggaattg gacgattgaa tatcccatct ccatcactat
     9541 caaagtatgg gaattgaatt gtttctaatt cgtatccacc ttctgccatt gataatgtag
     9601 ggttaatttt agaaccatct tctgtttcta gttttaagtt ccacttctta ccttcttccc
     9661 aacgttgacc cattgtgcca ttaggtacta ctaaactatc gctgattgca tcatgaataa
     9721 ctggcttcca ttcgccttgc tctgttgttt gacctaagtc actcgctctt aaaaatcgac
     9781 ccgctttata tccattttca tcttcatcca gcatgataag aaacggcata tctgtatatt
     9841 gtttagcgta atttataaag cgttcattag gttgattaac ataatgttct tgtaaaataa
     9901 catgcgtcat tccttgtgca attgcagcat ctgaaccagg attcggtgct agccagttat
     9961 ctgcaaattt cacattttct gcgtaatctg gtgctactga aatgactttt gtacctttat
    10021 agcggacttc agtcataaaa tgtgcatccg gagtacgtgt taaaggtaca ttagaccccc
    10081 acataataat gtatgatgcg ttataccagt cgcttgattc aggcacatct gtttgctctc
    10141 cccaaatttg tggagaggca ggtggtaaat ctgcatacca gtcataaaaa ctaagcattt
    10201 caccaccaag caaattgatg aatcgagcac ctgctgcata actaatcatt gacatcgctg
    10261 gaataggtgt aaatcctgcg attcgatctg gaccatattt ttttattgta tacagtaatt
    10321 gtgctgcgat tatctctgta acgtctttcc aatttgaacg cacgtgccct cccatacctc
    10381 gggcttgctt atattgtttg gctttgtctt cattttcaac aatagacgcc catgcagcaa
    10441 cgcgattacc attgttttct tctaatgctt cagtccataa atcccagagt tttccacgaa
    10501 tatatggata tttgattcga agcggactgt attcatacca agagaatgac gcacctcgtg
    10561 gacatcctct cggttcgtat tcaggcatat ccggaccaca acttggatag tcagtttgtt
    10621 gattttccca ggtaatcaca ccatttttca caaatacttt ccaagaacat gagcctgtac
    10681 agttaacacc atgtgttgtt cttacttctt tatcgtggct ccaacgttct ctgtacattt
    10741 tttcccattc tctactttta ctttctagga tcgaccaatt cccattaaat ttttctgttg
    10801 gcttaaagaa attcaatcca aattttccca tatttatatc ctcctacgta taaaaatacg
    10861 atgtgtagat gtcgtgtttt taaatacttt aaaatgccca agactattgc tttaattaga
    10921 ttgtacattt tttcacaaac ataaaatatt agggaatcac ctaattactt aaggaatttc
    10981 cctatcaata acgggatttc attgaaataa tacacaatca tgtatggtcg tgcttattgc
    11041 caatctaaat cgttcaaatt tggcacaacg acaaataagg cttcaacacg aatatattct
    11101 ctcggttgaa accttactta ttcatttatt tttttaaatt agtgacataa cactgtatta
    11161 gcatctgcac gatcggttga aatatatgtt acattttctt gctgcttaat aaatgcatca
    11221 tagtaatcat attgcgacga atgatatgtg ccattcgatg tattatttgg gtttagcaaa
    11281 cagccataac cttcgtcata taaatgttca cagagcataa gggcgtcatg tttagaacca
    11341 cttactacat aaaattgctg agtcggatta gaagttgttg agacattttc agtataacca
    11401 acaacttccc caataataca tatacctggt ttcgcctcaa ttgaatagtg ttgcaatttt
    11461 gaaataatat tacttaaacg ccccttaaca acaaactcgt taaaacacga tgcttgaaag
    11521 acaatcgcta tcgggtaatc aatatctgtg tattgttgta tctgtgtgat aattttccct
    11581 aaacgtttta cccccatata aattgctaac gtgccaccat tcactaagga attgacatcc
    11641 acttcatttt cttctgaatc tttaaagtga cctgtagaaa atgtcacact tttagcaact
    11701 gtacgcattg tcaaacctgt ctgcatagta gcaactgctg cgctcgctga tgtcacccct
    11761 ggtacaattt caaacgcaat atgatgttca tttagtgtgt cgacttcttc ttgcacacga
    11821 ccaaatatcg ctggatcgcc acctttaagt ctaacaacct tgttatatcg acgcgctgct
    11881 tccacgatac agtcatttat tttttcttgc tgaatatgtt ttgcatacgg ctttttacca
    11941 acatcgataa tttcagtagt caaattcgca tattgtaaaa ttaacggatt cactaatcga
    12001 tcatatagaa tgacatccgc ttcacgtatt aaacgctcag cctttttagt caaataattc
    12061 ggattacctg ggcccgcacc tatcaagtaa accttgccat attcctctac agacatatat
    12121 atacgttccc gtctgtaact tctacctcat aaacatctac acaaccttca tcaggttctt
    12181 gaacaatacc tgtatttaaa tcaatttttt gatcgtggag cgggcaaaat acatattccc
    12241 cactcactgt cccttcagac aatggtcctt gtttgtgtgg acagatattg tgaatcgcat
    12301 gaattttgcc actttctgtt aaaaacaatc ctacctcttt gtctttgaca ataacctttt
    12361 ttccaattag gggtgttaat tcatctatag ttgtcacttt aattttttct tttgtttcca
    12421 tgtattacac cttctccact tcaaaaattt tacgtgcttg cgcattgcta gttattgctt
    12481 cccaaggttc agcttcgact gcttttttag catccataat gcgttcaaat agttcatttt
    12541 gtctttctgg gtcaagtaag acttctttta cattttcaaa tccaagtctt cttaaccatg
    12601 gcgctgttct ttcagcatat atacctgttt cgcgataata ttgcatcaaa gcaccacata
    12661 atttgattac ttcatcttct gtttctacag ttgttaaaaa ttcagctttt tcaacttctg
    12721 taccaccatt accaccgata tagatttgga atccattttc aactgagata ataccaaaat
    12781 ctttaacacc tgattcaaca caacttcttg ggcagcctga tacacccatt ttgaatttat
    12841 gaggtgtatc gatgtattca aatgtttttt ctaaacgaat gccaagtcgt gtcgtgtatt
    12901 gcgtaccaaa tcgacaaaac tctttaccaa cacaactttt aactgagcga gttttcttac
    12961 cataagctga tgctgaacgc atacctaggt cttcccatat atttggtaat tcttcttttt
    13021 taactccata caaaccaaca cgttgcgaac ctgtcacttt aactagtggc acatgatatt
    13081 tcttagccac ttctcctaga cgaatcagtt ggtctgcatc tgtaacaccc ccacgcattt
    13141 gaggtataac agaaaatgta ccatcatttt gaatattcgc atggtaacgt tcgttagcaa
    13201 atcttgattc tctttcatct tcatgatcat gtggataaac catattcaaa taatagttaa
    13261 tcgcaggtcg acattttgga catccacctt tgtttttaaa gtttaaaaca tgtcgaactt
    13321 ctttagatgt ttttaaacct ttcgctctta tttgcgttac aatttgatcg cgtgtcaaat
    13381 cagtgcaacc acatatacca gcaggttttg cggcaacaaa gtcatctcct aaagtgtgct
    13441 gcaatatttg agcaatttgc ggtttacatt taccacatga attccccgct tttgttttag
    13501 ccgttacttc ttcaactgtt gtaaagccat tttccgtaat cgcatttact atagtacctt
    13561 tatcaacacc attacaacca caaattgttt catcatcagc catatcagca attgatagcg
    13621 atgcctcttc tccaccttta gtaagcaatg atacaagtgt gtaatcttca gtggattcac
    13681 cttttttcat catgttataa aagcgtgaac catcatcgat atcaccatat agtactgcac
    13741 caactacatt accgtctttt aaaaagattt ttttatagtt attatcaaca ctattaaata
    13801 tttcaatacc tttaatttct gcattttcta caatttgacc agcactatac aagtcacacc
    13861 cagaaacttt taatgacgta aatgttgttg atcccttgta tccgttcgtt tctttatttg
    13921 ttaaatgatc agctaatact ttaccttgtt catatagtgg tgcaacgagt ccataaactt
    13981 tgccgttatg ttctgcacat tcaccaactg catatacatt gctatcactt gtttgcatca
    14041 catcattgac aacaatacca cgattaacat ctagacctga ttctttggct acttctgtgt
    14101 atggtcgtat acctactgcc ataacaacta agtctgccgg aatctcgcgt ccatcagcca
    14161 atttaacacc ctcaacatca tcttctccta agatttcagt tgtgttggct tgcatttcaa
    14221 acttcatacc ttgcttttct agatctgctt taagcatatt tccagcttta cggtctagct
    14281 gcatttccat caaccattca gctaaatgta acaccgttac ttccatacct tgatctaata
    14341 aaccacgtgc acactctaaa cctagtaagc ctccaccaat tacaattgct ttctttttag
    14401 tcttcgcaat gttcatcatt tgttcagtat catcaatcgt tctccaacca attacactcg
    14461 gtaatgtcga acctggaact ggaatgacga atgctttaga acctgtcgca aaaatcaatt
    14521 tatcgtatga tacttcaata ccatttgcag tagtaactga ttgatttgct ctatctactt
    14581 caattacagg atcatttgta attaactcga taccatgttc ctcataccac tcatatggat
    14641 tcataattgt ttcttcaact gtcattttat tttgtaaaat atttgaaagc atgatgcggt
    14701 tatagtttgg ataaggttct ttacctatta ccgtaatatc atataaatcg ttggcgcgct
    14761 ctaatatttc ttcgattgtt cgaatgcccg ccataccgtt accaatcatt actagttttt
    14821 gctttgccat aaaatatgcc cctttactcc ataatattta tttcaaaaaa aggtattaat
    14881 ttttcgttag tgcttttata ttttcatcgg aatcattaag ctttctaatc tatcgttaat
    14941 gatttgcttt aaaattgggt cgaagttaat tgaaggtgtg atgtgtatat ctgtattaat
    15001 aaccatgtca ttcatttgct gcttcacttt gttaacaagc cttccgtcgt ataaaaataa
    15061 tggtacgaca atcaattttt gataccgttt cgagatgctt tctaaatcat gtgtaaaact
    15121 aatctctcca tatagcgttc tcgcatatgt cggcttgcta atttgcaaat tttgagtgca
    15181 tatttgtaac tcttcgtgtg ccttagtaaa ctttccatta atattgccgt gtgcaacaac
    15241 cataactcca acttgttgtt cttcacctgc taatgcgtca caaatacgtt gttcaattaa
    15301 tcgtctcatt aaaggatgtg tgccaagtgg ctcgcttact tctaccttta tgtctggata
    15361 ccgtcgtttc atttcatgaa cgatattcgg tatatccttg agataatgca ttgcactaaa
    15421 gattagcaat ggtacaattt taaaatggtc aaccccactt tgaatcaacg tcgtcattac
    15481 cgtctctaaa tcctgatgct cactttctaa aaacgcaata tcatagtgat gtatatcatc
    15541 ttttactaat tcagaaataa atgcttctaa cgcttgattc tgtcgtccgt gcctcatgcc
    15601 atgtgcaaca atgatattcc cattcacatt taccaaccct ttcacacgta ttgtatacca
    15661 aatcattttg tttttgtgaa aagaatcaca ttataatgta aaatcaggga attccctgat
    15721 gcctgtagtc atgcatattc cttatacatt ttcccttttt gttaaatcaa aaaagcgacc
    15781 gatatatgaa tccctactca acatttattt gagcaagcat taatatatcg gtcgcttgta
    15841 gtgtatatta ttatcttaaa atggtggttg gcctaatatt gtttcgtcaa agcgctcggg
    15901 tatcaatact ttgcgcatga tcacacctaa atcgccatca tcattttcat gttcgctgta
    15961 tatttcataa cctctttttt cataaatttt aagtaaccac ggatgcaatc ttgcagatgt
    16021 acctaaagta actgccgctg actttaacgt atctcgcaaa aatgcttctt caacataagt
    16081 aagtaattgg ctaccatagc ctttcccttc atactcagga tttgtcgcaa accaccagac
    16141 aaaaggatag cccgaaatac ttttcacact tccccaagga tatctaaccg taatcgtaga
    16201 tataatttca tcatcaattg tcatgacaaa tgtagtattt ttatctatat tttctttaac
    16261 agcatctaaa ttagcattaa ctgaaggcca atcaatacct agttctctta gaggcgtaaa
    16321 tgcttcatgc atgagttgtt gcaatttttc tgcatcttgt tcacttgcga gtcgaatcat
    16381 cattttttgt catattaatc cccactcttt tttaaatgat ttaaccatat tttattttta
    16441 aaataaatat ccatcaaagt gtatcaataa atttatcaca tgttagaaag tatgcttcat
    16501 ctgaatacac caatactctc ataaaactta ttaaaaatta ctctctcaac gtaaaaaaac
    16561 cattcaaatt catgaatggt ttggaagaat gattcattgt tacgctattt aatcactaca
    16621 tcttaattat tgttgctcta aacgattacg cttaccattt aagaaagcat aaacgagacc
    16681 tacaaaaata ccgccaccga caaagttacc taagaaagca aaaacgatat tttttaaaac
    16741 gtgtaaccat gaaactgcat caaggttaaa gaataccata cctgcatata gacctgcatt
    16801 gaacacaacg tgctcatatc ccatgtatac aaagaccacg acaccacaag ctataaagaa
    16861 tgcctttgtt aagccgcctt tgaattgcat agagatgaaa ataccaatat taataaagaa
    16921 gttacagaaa ataccttttg taaaaatatt caaccatgtt gaatcaacag tctttttctg
    16981 aactaaagct gttaaagctt gtgtcatttc aggtgtcatg acatgcgcaa atttcattaa
    17041 gaaaaataac acaaacccac ctaaaatatt tcctaagaaa caatataata aaatccaagt
    17101 catcttctta acagaaacga ctttataata ccagcctact gtaaagtaca tgaagttact
    17161 tgttaataat tcagagttag ttaatacaac taaaatcaaa cctaaactaa aagcaatggc
    17221 tcccattaaa ttgataagtc catctacgtg agtactcgca aactgcgtct ttattccgaa
    17281 cataaataca gtaacgattg agagtaaaaa tccggccatc attgctttaa gcatatatcg
    17341 gccaggtgtt tgctgtacca ttacttgctt catttgtact gaagatgata ttgaatcaac
    17401 aattgcgcct gttgaatatg tatcttcaac tgttttctta ttttctatca cagtacttcc
    17461 cccatttact ttgatcaaat aaaatttact ttctaattat attagtaaat caatgtaatt
    17521 gatagtttaa atttcagaaa aatcttttta ttctctcaaa aatgtagttt tctttcataa
    17581 aaacagcgag ataaatcgcg tgattggcta atatctttat gatttaagat attacgacgc
    17641 tcattttatc tcgctttatg taaagtatta cttattcttt tttaaatttg aaaagtaatg
    17701 taaaccatat gcaaatacac tgtaaccaat tgcagtacgt aaaataaacc ttataacttt
    17761 attaggtaca cattttgacg ttactgaaat agaagatagt agtgttgtga aaatatataa
    17821 tattaaaact ttgatataac gttctaccat cgaatcatcc ctcattttta taatattaca
    17881 agattattat aaaccgaatg aaacaaaaaa gtttttacat attttcaaca actttatttc
    17941 aataatgcat caagttgatc taaagtttga ttcatacctt gttcaacacc catgtttatg
    18001 acttgttgag cggcttcttt tgttggaaat accgatgttg atgtcactgt cgttttcgtt
    18061 gtattgctct tggaaaatga caaagtaatt ttcatgcttg gcatttttgt atcttttgca
    18121 ccttgaggtg tcgcaaatga gtcaatatat tcaattaaat atggacgctt aaccgtttta
    18181 tattctgcta atgtatagct ggtcattgta ggtgttttaa tcgcataaaa tgcatcaccg
    18241 cctgaaacag cattaaagcg aaacactttg gtactagcgt cttttggatg aaaccatttt
    18301 tcaaataatg cttttgtcgt ataggcatca aacacctttt caatcggtgc ttctatcgtt
    18361 cttgaaaaaa taattttatt atcttcaact ttaatcgtca tcttcccact ccctatcttt
    18421 tatatttact catcttaaca tgcattgagt aacatatgtt accaaatcat ttgttataaa
    18481 ctatagtaat attaatcatt ccctctgttg aaattgataa ctacgaaata aaaaacacac
    18541 tctattcagc tattaacagt tgagtagagt gtgcaaacct gttgaattaa tgcgctaaca
    18601 tttcttcttt gatttggtct ttatttaatt ttgaaggata atatgttggc cagttatcca
    18661 tttcttttaa taacgcgtca ttatcattac ccataaagat atggaagtgt tctgcctttt
    18721 taggtgcaat gttgtgatcg ctaaattgga tgaatttcgg taagtcttta tcattaccat
    18781 cgaccaattt aaacataaat ctcacgccac ggttaccttt aggatatttc aatgttttct
    18841 taccattgta ttcatattta ccagtgtgtt tcttaccatc tttagtaaat gtaatttcat
    18901 ttccttttat atcaatgtta gtaatatcag ttttatatcc tttgtcataa taagctttta
    18961 aatctttagc agatttcttc ggatcatttt cagctttatg ttccatcact tcatcaagcg
    19021 taccgtcttt taagtaaggg taaacagatt gccattcacc agcataatcg cttaattcac
    19081 ggtctttaac ttgctcatct ttaaaatacc catcagaaat tgctttgtca tgtttacttt
    19141 cagctttgtc gtctttcact ttaacaccac tgtctaaagc tttaccgata ttttcaatat
    19201 tagatttcat taatgattga tacgtaatat tatctttttt ctgttgttct ttatttaaag
    19261 actccatgtt gtagaatttt aaaggcttcg catctgtttc tttacgaatt gtttcagtca
    19321 ctttattcgc aacattatct tcgtaaagaa tatatttagc attgctatct ctaatttctt
    19381 taacaatttt agttaattct ttttgtgatg gatcttcagc attcatgttt tgaatacctt
    19441 tttgaacaaa accataacga tcagctaagt atccaattga ttcatgtgaa atgaatactg
    19501 cattaccttg cttatctttt gtaacttgct tcatatcgtt atcaattttc ttaagatcgt
    19561 cgtttaattt tttgtagttt ttctcatagt catctttatg ttttggatct tttttcacta
    19621 attcatcttt aatttcttta gcgaaagttt gattaatttt aggatctaac catacgtgtg
    19681 gatcatatcc accatgatga tgatgatgtt cttctttctc atgatcatgt ccctcatgtt
    19741 catgctcttc accatgctcg tgttgatcag ttaaaagctt tgctttatct aatttatcct
    19801 ctaaagacag ttttttatct ttatctttga tagtagatgc aactttctta gcaaccggat
    19861 ctaaattatc ccctgtatac ataaacaagt ctgacttgct tgcacttaat atatcttttt
    19921 gtgttggttc atagctatgt aaatctgtcc ctgctggata gattgatgat accttcacgt
    19981 gttttccacc aatttgctct gcaaatgatt gcaatggata aactgtcgtt ttaattgtta
    20041 ccttgccatc tttatctttt ccatcatcat tcccacatgc ggctaatgat aaagttacgg
    20101 ctggtacaag aagtaacata cctaatttct ttttcatagt caccctccta aatagtaatc
    20161 tttacgtttt aaaattatat actcatgcta cttaaaatac aagcactgtt tttataaata
    20221 atataaagtc gaatcatact ttaggtattt accacttttc gttttatcta atacatgact
    20281 attgtacaaa tatgatacag aaaactttat agtatttacg aacgtcataa tataagaaaa
    20341 aagacaatct ctatcatatt gctaatagaa attgcctttt catattgtcc taaaataact
    20401 taccctattt ttatagtgtg acttttagat ttaataatgg tatttacatg atgctatttt
    20461 tatatgattg tcatctactg tataaaccaa tctatgttcg tgattgatac gtctactata
    20521 atacccagtc agattcgatt ttaacttttc aggcttacct ataccttcca atgcaccatt
    20581 tctgtcaata cttttaagta actcattaat tttcttcacc atttttttat cgttctgctg
    20641 aaaatactta taatcttcaa aggcttgagg cgaaaacgta atatttaacc tagccattat
    20701 acatctatat ctttcgttat agttttccca cgttctagat ctgcaattga ttgagctaag
    20761 tgttcagcat tatttgggtt ctgttggagg taaagtgttt ccatcatgga gttataatct
    20821 gattctgaca tgattactac gtttttatca tcagtagatg ttacagttac catatcacta
    20881 tcatcattta cttttgtcat aagtgccttt aaattctgtc gagcgtatga ataattttta
    20941 ataatcatat ttatcgcctc cttttaacac acctcaattg tacaaatatc tgtacaataa
    21001 aacaagcgac acagaaaaaa actactaatc aagatttcgc acggggaaga atcaatacct
    21061 atcctgtact gattccctac taaacaatgt ttacatgata atttctacct atttagactt
    21121 catacattaa aaagacaatc tctatcatat cgctaataga aattgccttt tcatattgtc
    21181 ctaataaatt gatatcactt acttatctca acaattaaga ctattttatt ttatctttta
    21241 ataacttctc ataactttca taatcatatg gatcttcaac tttctcgcca ttaataaatg
    21301 cagttggtgt cgtttttata tgattatctt tcgctatttt tttatctttc tctgcagctt
    21361 tccaagactt gctatctttt gtcttgtaat cttttataat tttattttcc gtctctttat
    21421 ctaaatgcag ttgtttaata tgtttatcta atagttcttt tgttaaccat tctttatttt
    21481 catcttgctg ggcagcaaat aattgctttt gaaaatctaa aaatgattta ggtgcataca
    21541 tcaatactgc atgactcgca cgcgaaccaa caattgagtc tttacctaag aaagctaaat
    21601 tgacaaattg gtattccact ttgtgattat ctatataatt tttacgcaac tttggcatga
    21661 ctttttcatc taattcttta caataaggac atttatagtc gccatatatg acaactaatg
    21721 gtttgccgtt tttcgaggat gtcgttgctg attctttttt accgcaagct gttaaaatta
    21781 acatgctcac tataaataat gttagtaatt ttttagtcat aataactcct gttcatatct
    21841 agttcgtcat attttcttca tgataatccg catcatgttg ttgataatat cgtctcgaat
    21901 caaaatcttg cttatatgat acacgtttat cactaaaatc atatgcataa taatgcactt
    21961 catcatcatt actcaaaggt ttataagcta aaatgataat tttatcctta tcatagacat
    22021 aaatattagc atctttgcgt tcaaaatgat caacacgatc accgttgcct tttttggcaa
    22081 gttgttcttg tttttgattt tgaattttaa ctgcttcatc tatttctttc acatatttat
    22141 cattaccaca tcctgatata aagactagta ataataatac cgtcgccaca aatcgcttca
    22201 atgctcccac tcctactatc aacttccaac taactttcaa tataattaat actatacacc
    22261 gtcaaacatt aatgcgctac aactactacc aatgcattta atataaatat ccaaagaaat
    22321 aacagttatg tatagtcaaa gaaaaatata tttcattttc tatgtgtatt tgtaatcagt
    22381 ttttgataaa ataaaaaaat agcattatta ataaattcaa aaattacagt tcaattcaga
    22441 gagggatggg ataaaatgaa ctttgtaacg ttgacttcgg atgagttcaa tgcgtttaca
    22501 acaaagcatt tttcacatta cacacaatca actattcatt acaatcatag agttgattta
    22561 aaaggcgatg tgcatcttgt aggggttaaa gatgacaatg gtcaagtgat tgcaggatgc
    22621 ttattgacag aagcacgcac acttaaattt ttcaaatatt tttatacaca tcgcgggcca
    22681 gtgatggatt atacaaatca atcattagta gcatttttct ttaaagcatt aacgtcatat
    22741 ttaaagaaac acaattgttt atatgtcctt gtagatccat atttaattga aaatttacgc
    22801 aatgcagacg gtgaaattgt taaatcttat gataaccgag catttgttag aacaatggat
    22861 aaattaggtt ataaacacca aggtttccct gtaggttatg attcaatgag ccaaatccgt
    22921 tggctgtcag tgttagattt aaaagataag actgaagacc aacttttaaa agaaatggat
    22981 tatcaaacga gacgtaatat taaaaaaaca tatgatattg gtgtcaaaac taaaacgtta
    23041 acgattgatg aaacgcaaac ttttttcgac ttattccata tggctgagga aaagcatggt
    23101 ttcaaattcc gtgagttacc atactttgaa gaaatgcaaa agttatacga tgaccacgcc
    23161 atgttaaagt tggcgtatat tgatttaaac gagtatttaa aaacgttaca attaaagcaa
    23221 caacaattaa cagctgaact ctcaggtgtt gaagaagcat tggaagaaag tcctaattca
    23281 aagaaaaata aaacgaaacg cacacagcta gaacaacagc taaatagcaa taagcgtaaa
    23341 atcgacaata caatagaaca aattgaacaa gatggtgcag tcttaaactt agcttctgct
    23401 ttgtttatct acaatgagca tgaagtttat tacttatcta gtggttcaaa ccctaaatat
    23461 aatgcttata tgggtgccta ccatttgcag tgggaaatga ttaagtttgc taaagcgcat
    23521 catattgatc gctataattt ttatggtatt accggtgact tttcagagtc atcagaagat
    23581 tatggagtcc aacaattcaa aaaaggtttt aacgcacatg tagaagagta tatcggcgac
    23641 tttattaagc ctatcaaacc attactatat aaggtacaaa catacttaaa tcataaacgt
    23701 cgttaaaata tcgctccaag attatgacaa cataacaaag aaccacgcat catgtatatg
    23761 gcatcatcac catactaaaa catgatacgt ggttttattt gattattctt catttataac
    23821 atttaagaaa cgtcttaatt cttctgtttt cggacgattg aacatctctt ctggagcctc
    23881 ttgttctccg atcatgcctt catgaataaa tacaatgtta ttagatactt ctttagcaaa
    23941 acgcatttca tgtgtcacaa tgaccattgt catgccttca ttagccaaat ctttaataac
    24001 ctttaaaaca tcattcacaa gttcaggatc aagtgcagat gttggttcat caaacaacat
    24061 cactttaggg ttcattgcta gtgctcttgc aatagcaaca cgttgttgtt gaccacctga
    24121 taatgcatgt ggacgttgat ctttgacatg tgttaaacca actttctcaa gtaactcaag
    24181 tgatttccca cgtgcctcat cctttttcaa ctttttaact gtgataagac cttccattac
    24241 attttctaat gccgtcttat gcggaaaaag gttataactt tgaaatacca taccagactg
    24301 tttacgaact tctatttgtg attttttatc tttagatgta tatgttttgc cgttaacata
    24361 aactgtacct tcagttggaa tttctaatgc attaatcata cgtaacaatg ttgttttacc
    24421 tgaaccagat cgaccgatta aggttacaac ctcaccttgt tcaacagata aatcaatacc
    24481 tttgatgact tcaacatcat tgaatgattt atggatattg ttcaattgaa tcatgagcga
    24541 taccctcttt caatataaga ttcatagata ccttggataa ttgaaataat aaagcatacc
    24601 acccagtaca ttagcgcaac taacacataa attgttaaat attcatatgt tgtagacgca
    24661 acttcttgag cttttctaaa catttcagcc actaaaataa atcctaataa tgatgtatct
    24721 ttgattaaac ttaaaaatgt attacctagt gcaggaatcg acactcgaat tgcctgcggt
    24781 aaaatgatac gttgtatcgt ttgaccatac gtcataccga tggaatatgc agcttctgtt
    24841 tgtcctttcg gtatagaaat aataccgcca cgaataattt ccgaagcata cgcacctaca
    24901 tttaatgata aaccaataat agcagccact acaggtgcca atgtccattg gttgtcagcg
    24961 tcatttgtta ataatctacc taattctgga ataccataaa atatgataaa tagttgtact
    25021 atcataggtg ttcctcgaat aatagataca tagacacgtg aaatacttct taaaatttta
    25081 ctcgttgaaa ttcgcattaa tgctgtaaat aatgcaataa tcaatcctaa aacaaatgta
    25141 actaatgtaa taggaattga atatttgacc aaaccttcta gcataggtcc gaaagcttgt
    25201 tttgcagcat ctagcgcatg ttgttgttcg ctatttagat ttagaaacat cttgaccaaa
    25261 ccatttctta cctattttag ctaattcacc gttttcctcg atttttttca agccatcatt
    25321 gaatttttga actgtttcat catctgcttt tttagaaaat gcaaatgcag atctactttg
    25381 ttcagcatta cctttgattg ctttgatctt agcattaggt ttttgttttt tataatccaa
    25441 gtatgacaga ctatcattaa atgtaccatc aacacgctta gacaataata aatccattga
    25501 ttggttaaag ccatcaactt ttgtaatatc agcaccttta tccttagcta atttaccata
    25561 attagatgtg aatgtttgtg ctaacttctt acctttaaca tcatcaaaat ctttaatatc
    25621 tttttcattt tcacgaataa ctaaaacagc acttgagaat gtgtaaggct tagaaaattt
    25681 atatttcttt tctctatcag gattaatacc tacttggttc gcgattacat caaaacgccc
    25741 tgcgtctaaa cctgcaaaca tagaatccca agaagtttca ttaaatttaa gttttaaacc
    25801 ttcttcttta gccactgctt taataacatc aatatcgtaa ccagttaatt taccatcttt
    25861 atcgtggaaa ctaaatggtg cataagtccc ctcagtccca acattaattg tcttgctatc
    25921 tttactagct tccttgtctt tcgacgaatt gtttccgcat gcagccaata tgaaaacgaa
    25981 agctatcatc acaaataaaa gtcttttcat agaatatatc tccttattct tattattcta
    26041 atcggaataa aaatatttta atctgaaatg ccggataagt caatatgttg aacttttgct
    26101 aaaaagcttt atatagctag caacttatat ttattgacga gaatcaactt cttttttctt
    26161 cccttgtaac ataaatgaac aaaagaaact aataactgta atcaataatg caaataaaaa
    26221 tccagcgtga taaccatgca acatcgtatc aactttaatt tgattcatca tatcaatttt
    26281 gttcatgcca ttatagtctg acatagttgg agaaaagttt ttgcttgcat gagataaaat
    26341 agtaattaat gcagctgttc cgatagaacc agcaatttgt tgtactgtgt ttgtcataga
    26401 tgaaccatgt gcattcattt ctggctttaa ttgattcatc gtatgggtca ttactggaat
    26461 taatcctaac gcgataccaa ccattcgaat cgcataaact gttgccaaca tgattgttga
    26521 tgtttgttca tccattacaa caaaataagc cgtagtaata acaacaatcg ccataccgac
    26581 taaagccaat tttctaggac caaacttttc atataaagca cccgaagtca tcgacataaa
    26641 tgccataaca attgctcctg gtaataaagt taacccagaa tctaaagctg aacgatgcaa
    26701 taaattttgc acaaagatag gtaaaatcgt ttccgaacca atcatcgata ccattgtgac
    26761 acccataata atgataccaa ctgtaaattg tttattttta aatacagcaa aatttaataa
    26821 tggtgttgat agccgatatt gacgtaatac aaataatgcg actaaaacaa cgcctccaat
    26881 catagataat aatacgatag gattatcaaa acctttttct gaaattgaac tcgttccata
    26941 caataacccg ccgaaaccta gtacagaaaa aatgacagaa ataaaatcaa taggtacttt
    27001 aattgtagtt ccaacatttt ttatcgtttt aaatccaatt aaaaatgtca cagctgcaat
    27061 tggagcgaca acatggaaaa gcgatctcca gttaaaatat tcaacaaggt aacctgaaag
    27121 agtaggtcca atagctggtg ctaaaccaat taccaaacca aatatcccca tatatttacc
    27181 gcgttcatgc ggttggaaaa catccaaaat cgttgtcatc atcaatggca tcataatacc
    27241 tgcgcctaac gcctgaataa ttctagcaac taataatgtt gcaaagtgcg gactcaagcc
    27301 gccgagtaat gtacctaaga aaaagataga aatacccact aaaaacactt gtcttgttgt
    27361 ataacgttgt ataacaagcg cggacaaagg aataacaatc ccattagtca ataaaaaagc
    27421 tgttgtcagc cattgaactt gtgtatatga aatttcaaaa tctttcataa tacttggtaa
    27481 agccgtcact aatagtgttt catttaataa tccaaaaaat ccacccatca gcatagtaat
    27541 aatcatcgtt aatttttgtt tcttcgacat ttaaagcttc cccctcacca tctaaaaaac
    27601 gacacacatt tttagatatc cggtaattat acccattttc caaaaaatta gcacgacttt
    27661 attacaattt ctttaaaacg taatcattac tatttacata tgagtagtta tcggtcataa
    27721 tatatttaca aggagggata gagatgaaat tagatttaca gactgctcgt cgaaatttaa
    27781 atagccctaa cattaaaaca agaaagcgtg ctttaaagat tattaagcaa cacaaaagag
    27841 cgaaataatt gttttaattt gactatattg tatgtctaac tatattttcg agtatcccta
    27901 gctcgttaat ataaactcta tacttaacta tttccttaca ttacttcaca tatttaagag
    27961 ctgcatatac actcactcct tgtgtacatg cagctctttt tttataagta gtatttatct
    28021 ataacttcta aatcatccgt taattcataa acaagcggtg cacctgtttt aatttcataa
    28081 ttaatgatat cttcatctga cacatcttca agatatttaa ttaatgcgcg aattgaattt
    28141 ccgtgtgcag aaactaatac cgtttgacca tctagcaaat attgtgaaat atgatctgtc
    28201 caaaatggta tcactcgaac taaagtatct ttcagacttt cagaataagg catcatacgt
    28261 ttatctaaat gattatatcg acgatcagct aagtaagctt cacgttgttc ttcggtttca
    28321 gcaggtggtt tcacatcata agaacgacgc caaatatgta cttgttcttc tccaaattct
    28381 tttctagcat catctttatt taagccttgc aatccaccat agtggcgttc atttaaacgc
    28441 cagcttttat atacaggaat ccattgttgt ttagattcag ttaaaatata atgcgttgta
    28501 tctaaagcac gtgttaataa cgatgtaaaa gctacatcga tggcaatgtt attttctctt
    28561 actttttcac ctgctctagt cgcttcatta ataccttgtt cagataaatt aacatcttcc
    28621 catccagtaa ataagttttt agcattccac tcgctttgtc catgacgaca taaaattaat
    28681 tttggcataa cttataacct ctttccttta atctttagga cgttatttca gggatatttt
    28741 aagatgattt aatcttagta attttctttt caatatattt taaaattcaa tgattaaatc
    28801 aattatcact agacattaaa tacataaatc ctattcccca ttttcatttt ttaattcata
    28861 aatgaatcaa taaccaccta ataacaaatc atattataca cctttgttcg ctatttttct
    28921 aaggtttaaa aaatattttt aggtaagcct aaaaatagat gtaataaaaa cgcctcctca
    28981 gatatttata tatctatgaa gacgtttaaa tacattatag atggtctggt tctgggtgaa
    29041 cgtatactga ggaaatacct tttttgtgca aatgatgttc gacattgtca caaatttgat
    29101 gcgcttctac taaggaaagg ttagcatcta caacaattgt gacatcaata aacacactac
    29161 ttccatggta acgcccttta atacttttaa cttcttgtac ttcatcaact tctaaaatat
    29221 cattgcgata cgcttctaat tcagtttcat tgaaaccatc actcaacata aaaattgctt
    29281 ctttaaaaat accaaaacca gtataaacga ttagtaagcc tagtaatgtt gctaaaataa
    29341 tatcgacaat tgggaaaccg atttgcgtaa aaattaatcc tatcgctgtt ccaatgctga
    29401 ctaaactatc tgataaatta tctttggcag ccgaatttaa agaactactt ttcgttcttt
    29461 tcgctagtct ttgattgact gcaaatacaa tcaacattac aagaccactg attaagctga
    29521 cgataattgt tattgcgtta ggtacaacgt catcttcttt gaacaaacga ggtgcatttt
    29581 gaataactac ttggatacct acaaacataa tgacaaatga caccaataat gaagaaatat
    29641 tttcagactt caaatggcca taaggatgat ttcgatctgc aggtttaatt gaaattttca
    29701 atccaataat aacagctaaa gaaacgataa tatctgtcat attgtttaat gcgtcggctc
    29761 ttacagctgc agagttaaag acaaaacccg tgacatactt aacaatagat aagattatat
    29821 atacaattaa actcaaataa gcaccgcgtt gcgccaattt aagattttca ttatgagaca
    29881 tgcgttgaac caccttgaat tagtatagta acaatattat gaatgattta ttttaaattt
    29941 acaacgtttt taatttttat aaatttttat aaaattaaac taatttattc attcgcaacc
    30001 cctaaaaata atttttagcc tttctgcgaa ttttatgagc tagaaaggct tatgcattag
    30061 tagcgaagct aactaatgcg ttcctttatt ttctgcgaat tttatgagct agaaaggctt
    30121 atgcagttga cgttttacgt ccaactgcgt tccttcgtct tcttcaaatt tatttgttag
    30181 aaaggtgccc aacccttcct gcttgttaac tttcgtctcg aaaatttcta tgttgggacc
    30241 ctatgcatta gttgcgaagc tatctaatgt catgacctaa ttatatagaa gaaaaagtgc
    30301 atcagtgaca aattaaatga gatttctact ctaccaaact ctcttcgaaa gacaattttc
    30361 tcctctattt attagcaaac tattgcattt ctccatatag tacttcctta cttaaaatac
    30421 gctgaatgtt ctgaattaaa cgctttttta catagttaac actagttaat ctattagtta
    30481 acattagtta ataattagtt aatttccatt tgtattctca tgtgataaat tctaaaagca
    30541 tacaataaat ttaatatgta aaaagaaagg gaatacacat gaaaaataaa tatatctcga
    30601 agttgctagt tggggcagca acaattactt tagctacaat gatttcaaat ggggaagcaa
    30661 aagcgagtga aaacacgcaa caaacttcaa ctaagcacca aacaactcaa aacaactacg
    30721 taacagatca acaaaaagct ttttatcaag tattacatct aaaaggtatc acagaagaac
    30781 aacgtaacca atacatcaaa acattacgcg aacacccaga acgtgcacaa gaagtattct
    30841 ctgaatcact taaagacagc aagaacccag accgacgtgt tgcacaacaa aacgcttttt
    30901 acaatgttct taaaaatgat aacttaactg aacaagaaaa aaataattac attgcacaaa
    30961 ttaaagaaaa ccctgataga agccaacaag tttgggtaga atcagtacaa tcttctaaag
    31021 ctaaagaacg tcaaaatatt gaaaatgcgg ataaagcaat taaagatttc caagataaca
    31081 aagcaccaca cgataaatca gcagcatatg aagctaactc aaaattacct aaagatttac
    31141 gtgataaaaa taaccgcttt gtagaaaaag tttcaattga aaaagcaatc gttcgtcatg
    31201 atgagcgtgt gaaatcagca aatgatgcaa tctcaaaatt aaatgtaaaa gattcaattg
    31261 aaaacagacg tttagcacaa cgtgaagtta acaaagcacc tatggatgta aaagagcatt
    31321 tacagaaaca attagacgca ttagtagctc aaaaagatgc tgaaaagaaa gtggcgccaa
    31381 aagttgaggc tcctcaaatt caatcaccac aaattgaaaa acctaaagca gaatcaccaa
    31441 aagttgaagt ccctcaaatc caatcaccaa aagttgaggt tcctcaatct aaattattag
    31501 gttactacca atcattaaaa gattcattta actatggtta caagtattta acagatactt
    31561 ataaaagcta taaagaaaaa tatgatacag caaagtacta ctataatacg tactataaat
    31621 acaaaggtgc gattgacaaa gctgtattaa ctttacttgg cgatggttct aaatcttata
    31681 tccaaccatt gaaagttgat gataaaaatg gctatttagc taaatcatat gcacaagtaa
    31741 gaaactatgt aactgagtca atcaatactg gtaaagtatt atatactttc taccaaaacc
    31801 caacattagt aaaaacagct attaaagctc aagaaactgc atcatcaatc aaaaatacaa
    31861 taactggatt atttaactca ttctggaaat aatcaatcaa aaatatcttc tctagtttta
    31921 catcattttt taaataattt tcgtaataaa ccgtgattaa aaagaaccgc tgattctcaa
    31981 tcgaatctac ggttcttttt tcattttcca tcaattaaat gtttcttcgc tatttgtcag
    32041 cccacttttt tacctgcaac ttgttaaata atccttacat cgttaacgaa tagttcatca
    32101 tttagttgaa tcagctcaac tttattaact tcaaattttc acaaactatt gcgcaatcca
    32161 ttcctcttcc actacaagca ccataattaa acaacaattc aatagaataa gacttgcaaa
    32221 acatagttat gtagctatat aaacgcctgc gaccaataaa tcttttaaac ataacataat
    32281 gcaaaaacat catttaacaa tgctaaaaat gtctcttcaa tacatgttga tagtaattaa
    32341 cttttaacga acagttaatt cgaaaacgct tacaaataga ttattatata tatgaactta
    32401 aaattaaata gaaagaaagt gatttctatg attaaaaata aaatattaac agcaacttta
    32461 gcagttggtt taatagcccc tttagccaat ccatttatag aaatttctaa agcagaaaat
    32521 aagatagaag atatcggcca aggtgcagaa atcatcaaaa gaacacaaga cattactagc
    32581 aaacgattag ctataactca aaacattcaa tttgattttg taaaagataa aaaatataac
    32641 aaagatgccc tagttgttaa gatgcaaggc ttcatcagct ctagaacaac atattcagac
    32701 ttaaaaaaat atccatatat taaaagaatg atatggccat ttcaatataa tatcagtttg
    32761 aaaacgaaag actctaatgt tgatttaatc aattatcttc ctaaaaataa aattgattca
    32821 gcagatgtta gtcagaaatt aggctataat atcggcggaa acttccaatc agcgccatca
    32881 atcggaggca gtggctcatt caactactct aaaacaatta gttataatca aaaaaactat
    32941 gttactgaag tagaaagtca gaactctaaa ggtgttaaat ggggagttaa agcaaattca
    33001 ttcgttacac cgaatggtca agtatctgca tatgatcaat acttatttgc acaagaccca
    33061 actggtccag cagcaagaga ctatttcgtc ccagataatc aattacctcc tttaattcaa
    33121 agtggcttta atccatcatt tattacaaca ttgtcacacg aaagaggtaa aggtgataaa
    33181 agcgagtttg aaatcactta cggcagaaac atggatgcta catatgctta cgtgacaaga
    33241 catcgtttag ccgttgatag aaaacatgat gcttttaaaa accgaaacgt tacagttaaa
    33301 tatgaagtga actggaaaac acatgaagta aaaattaaaa gcatcacacc taagtaaaca
    33361 gttcaatcat tttataaaat cctgggacac ttcatacttg tctcaggatt ttttaacata
    33421 ttgaatcagc ctcataacat taaattaatt tatcgtatat taaatttaat aataacaact
    33481 gatttttata agaacaaagt atcacaccat agtagataca caaataatac aaatgaatcc
    33541 atttaagttg aaagcttaaa taaatattct caagttaata aataattaac ttttagatgg
    33601 attcatcaaa aatcgtaaaa agaacaattt gtattttaca aacattaatt aaaaataaaa
    33661 gcaagacatt cgtgcaatcg gttaccttaa attgtttaca actgtcaaca ataccaaggt
    33721 tttattaact atatttctca caaaattagc ttttagcatt ccaaacaaaa aaggttaaat
    33781 tgaacggaat tatggcattt ttaacttaat tgtaaaaaaa agttgataat ggtcaattgt
    33841 ta
//
LOCUS       DAMZKC010000007        33704 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.007, whole genome shotgun sequence.
ACCESSION   DAMZKC010000007 DAMZKC010000000
VERSION     DAMZKC010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 33704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33704
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.007"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            36..1742
                     /gene="acsA"
                     /locus_tag="POB55_000246"
     CDS             36..1742
                     /gene="acsA"
                     /locus_tag="POB55_000246"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HDI6499242.1"
                     /translation="MKVEVYKGAQGKHNLKDYEEIYNTFDWKDVEQAFSWSETGKMNM
                     AYECIDRHVDQGLGDKIALNYKDEYRKESYTYKDMQRLSNKAANVLSEHAEVDKGDRV
                     FIFMSRTPELYFALLGVLKIGAIVGPLFEAFMEKAVADRLENSEAKVLITNKALLPRV
                     PVDKLPNLKKIVVVDEDVEDNYIDFISLMETASDEFDIEWLKSDDGLILHYTSGSTGQ
                     PKGVLHVQQAMLVHYISGKYVLDLQEDDVYWCTADPGWVTGTSYGIFAPWLNGATNCI
                     AGGRFSPEQWYSMIEDFKVTIWYTAPTALRMLMSAGDDIVEKYDLSSLRSILSVGEPL
                     NPEVIKWAKKVYGLTVLDTWWMTETGGHMIVNYPTMDVKLGSMGKPLPGIQAAIIDDA
                     GNELPPNRMGNLAIKKGWPSMMYRIWKNPEKYKSYFIGDWYVSGDSAYKDEDGYFWFQ
                     GRVDDVIMTAGERVGPFEVESKLVEHEAVAEAGIIGKPDPVRGEIIKAFVALRKGYEP
                     TDELKEEIRLFVKEGLSAHAAPREIEFKDKLPKTRSGKIMRRVLKAWELNLDAGDLST
                     ME"
     gene            2182..3849
                     /locus_tag="POB55_000247"
     CDS             2182..3849
                     /locus_tag="POB55_000247"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HDI6499243.1"
                     /translation="MTHLSDLDIANQSTLQPIKDIAASVGISEDALEPYGHYKAKIDI
                     NKITPRENKGKVVLVTAMSPTPAGEGKSTVTVGLADAFHELNKNVMVALREPALGPTF
                     GIKGGATGGGYAQVLPMEDINLHFNGDFHAITTANNALSAFIDNHIHQGNELGIDQRR
                     IEWKRVLDMNDRALRHVNVGLGGPTNGVPREDGFNITVASEIMAILCLSRSIKDLKDK
                     ISRITIGYTRDRKPVTVADLKVQGALAMILKDAIKPNLVQSIEGTPALVHGGPFANIA
                     HGCNSILATETARDLADIVVTEAGFGSDLGAEKFMDIKAREAGFDPAAVVVVATIRAL
                     KMHGGVAKDNLKEENVEAVKAGIVNLERHVNNIKKFGVEPVVAINAFIHDTDAEVEYV
                     KSWAKENNVRIALTEVWEKGGKGGVDLANEVLEVIDQPNSFKPLYELELPLEQKIEKI
                     VTEIYGGSKVTFSSKAQKQLKQFKENGWDNYPVCMAKTQYSFSDDQTLLGAPSGFEIT
                     IRELEAKTGAGFIVALTGAIMTMPGLPKKPAALNMDVTDDGHAIGLF"
     gene            complement(4095..4184)
                     /locus_tag="POB55_000248"
     CDS             complement(4095..4184)
                     /locus_tag="POB55_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499244.1"
                     /translation="MLLTTPIRLYLIDNNYQYQLLVLIVAHPN"
     gene            4183..6870
                     /gene="harA"
                     /locus_tag="POB55_000249"
     CDS             4183..6870
                     /gene="harA"
                     /locus_tag="POB55_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haptoglobin-binding heme uptake protein HarA"
                     /protein_id="HDI6499245.1"
                     /translation="MNKHHPKLRSFYSIRKSILGVASVIVSTLFLITSQHQAQAAENT
                     NTSDKISENQNNNATTTQPPKDTNQTQPATQPANTAKTYPAADESLKDAIKDPALENK
                     EHDIGPREQVNFQLLDKNNETQYYHFFSIKDPADVYYTKKKAEVELDINTASTWKKFE
                     VYENNQKLPVRLVSYSPVPEDHAYIRFPVSDGTQELKIVSSTQIDDGEETNYDYTKLV
                     FAKPIYNDPSLVKSDTNDAVVTNDQSSSDASNQTNTNTSNQNTSTINNANNQPQATTN
                     MSQPAQPKSSANADQASSQPAHETNSNGNTNDKTNESSNQSDVNQQYPPADESLQDAI
                     KNPAIIDKEHTADNWRPIDFQMKNDKGERQFYHYASTVEPATVIFTKTGPIIELGLKT
                     ASTWKKFEVYEGDKKLPVELVSYDSDKDYAYIRFPVSNGTREVKIVSSIEYGENIHED
                     YDYTLMVFAQPITNNPDDYVDEETYNLQKLLAPYHKAKTLERQVYELEKLQEKLPEKY
                     KAEYKKKLDQTRVELADQVKSAVTEFENVTPTNDQLTDVQEAHFVVFESEENSESVMD
                     GFVEHPFYTATLNGQKYVVMKTKDDSYWKDLIVEGKRVTTVSKDPKNNSRTLIFPYIP
                     DKAVYNAIVKVVVANIGYEGQYHVRIINQDINTKDDDTSQNNTSEPLNVQTGQEGKVA
                     DTDVAENSSTATNPKDASDKADVIEPDSDVVKDADNNIDKDVQHDVDHLSDMSDNNHF
                     DKYDLKEMDTQIAKDTDRNVDKGADNSVGMSSNVDTDKDSNKNKDKVIQLNHIADKNN
                     HNGKAAKLDVVKQNYNNTDKVTDKKTTEHLPSDIHKTVDKTVKTKEKAGTPSKENKLS
                     QSKMLPKTGETTSSQSWWGLYALLGMLALFIPKFRKESK"
     gene            7001..7093
                     /locus_tag="POB55_000250"
     CDS             7001..7093
                     /locus_tag="POB55_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499246.1"
                     /translation="MKIILLLFLIFGFIVVVTLKSEHQLTLFSI"
     gene            complement(7073..7978)
                     /locus_tag="POB55_000251"
     CDS             complement(7073..7978)
                     /locus_tag="POB55_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDI6499247.1"
                     /translation="MTNQDNNHQLNHRIYHFEKIYKAIKHVIVFIFMIFIAIVAIAVI
                     AMSLYFHHLTKTSDSLSDDALIKKVRQIPGDELLDHNNKNLLYEYNHSQNSLIIGPKT
                     SSPNVIKALTSSEDTLFYKHDGILPKAILRAMIQDIFNTDQSSGGSTITQQLVKNQVL
                     TNEKTYSRKANELRLAIRLEHLLSKDEIIYTYLNIVPFGRDYNGANISGIASASYSLF
                     GIPPKDLSIAQSAYLIGLLQSPYGYTPYEKDGTLKSDKDLKYSIQRQHYVLKRMLIED
                     QITEKEYNDALKYDIKSHLLNRKKR"
     gene            8360..9622
                     /locus_tag="POB55_000252"
     CDS             8360..9622
                     /locus_tag="POB55_000252"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HDI6499248.1"
                     /translation="MTNVLIEDLKWRGLIYQQTDEQGIEDLLNKEQVTLYCGADPTAD
                     SLHIGHLLPFLTLRRFQEHGHRPIVLIGGGTGMIGDPSGKSEERVLQTEEQVDKNIEG
                     ISKQMHNIFEFGTDHGAVLVNNRDWLGQISLISFLRDYGKHVGVNYMLGKDSIQSRLE
                     HGISYTEFTYTILQAIDFGHLNRELNCKIQVGGSDQWGNITSGIELMRRMYGQTDAYG
                     LTIPLVTKSDGKKFGKSESGAVWLDAEKTSPYEFYQFWINQSDEDVIKFLKYFTFLGK
                     EEIDRLEQSKNEAPHLREAQKTLAEEVTKFIHGEDALNDAIRISQALFSGDLKSLSAK
                     ELKDGFKDVPQVTLSNDTTNIVEVLIETGISPSKRQAREDVNNGAIYINGERQQDVNY
                     ALAPEDKIDGEFTIIRRGKKKYFMVNYQ"
     gene            complement(9716..10990)
                     /locus_tag="POB55_000253"
     CDS             complement(9716..10990)
                     /locus_tag="POB55_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDI6499249.1"
                     /translation="MSDFNHTDHSTTNHSQTPRYRRPKFPWFKTVIVALIAGIIGALL
                     VLGIGKVLNSTILNKDGSTVQTTNNKGGNQLDGQSKKFGTVHEMIKSVSPTIVGVINM
                     QKASSVDDLLKGKSSKPSEAGVGSGVIYQINNNSAYIVTNNHVIDGANEIRVQLHNKK
                     QVKAKLVGKDAVTDIAVLKIENTKGIKAIQFANSSKVQTGDSVFAMGNPLGLQFANSV
                     TSGIISASERTIDAETTGGNTKVSVLQTDAAINPGNSGGALVDINGNLVGINSMKIAA
                     TQVEGIGFAIPSNEVKVTIEQLVKHGKIDRPSIGIGLINLKDIPEEEREQLHTDREDG
                     IYVAKADSDIDLKKGDIITEIDGKKIKDDVDLRSYLYENKKPGESVTVTVIRDGKTKE
                     VKVKLKQQKEQPKRQSRSERQSPGQGDRDFFR"
     gene            11162..11779
                     /locus_tag="POB55_000254"
     CDS             11162..11779
                     /locus_tag="POB55_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HDI6499250.1"
                     /translation="MYSVISKILNFILVKMSKSLYVIGKDNIPKDSKYVVTCTHESYN
                     EVIMLGMALYPNQIHYMAKKELFKNKWIGKFLTSLNAFPVDRENPGPSTLKRPINLLK
                     DNKTVGIFPTGSRTSQEGAPLKRGASTIAMLSKSPILPVAYVGPTKIHGLLTGQAYIN
                     IGKPIDINDLPKDLKRNERIDYITKEIETRTAKLQQELHEIVKSL"
     gene            11907..13373
                     /locus_tag="POB55_000255"
     CDS             11907..13373
                     /locus_tag="POB55_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIBC"
                     /protein_id="HDI6499251.1"
                     /translation="MYKFFQNLGRSLMLPVAILPAAAIIAGIGNTLNALHATPKIAMF
                     FTTVGTTILEQLGILFAIGVAIGMAKKNDGAVALAATLGYFLVTVVLSPMKLAPLLGM
                     KASEINSAFEKMNNGNVFVGIVIGLIAAYAYNKFSEIELPLALSFFSGKRLVPIMTAF
                     YCTFLVVILLFLWPLLYSWIVKFGESIVGLGSFGAFIYGVANRLLIPTGLHHALNSVF
                     WFDTIGINDIGKFQSGKDAIKGITGRYQAGFFPIMMFGIPAAALAMYHTAKTTQKKQV
                     YGWFLASSVAAFFVGVTEPIEFAFMFVAPILYVVHALLTGLSLFIAATFHWTAGFSFS
                     AGLIDYVLSLINPVSNHPLMLLVQGVVFFILYYVIFRVVIQVFNLNTIGRGENELVDP
                     TVVKDNIAPGENDIKQSKYHQHAIQILEGLGGQENIVNLTNCATRLRLELKDTSIIDQ
                     QKIKNAGAVGVTVNGKHSTQVIVGTHVQQVADEIEKHL"
     gene            13480..14610
                     /locus_tag="POB55_000256"
     CDS             13480..14610
                     /locus_tag="POB55_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDI6499252.1"
                     /translation="MKKILFDVDGVFLSEERCFDVSALTVYELLMDKCYLGLHSHIDW
                     ETLTDNDIQDIRNRIFQKDKILNKLKSLGLNSNWDMLFIVFSIHLIDILKKLSHDEIE
                     AFMYQDEPVELKLQNISTNLADCFNLNEQLPLQFLDNVKVGKNNIYAALEEFATTELH
                     VSDATLFSLKGALWTLAQEVYQEWYLGSKLYEDVEKKIARTTFKTGYIYQEIILRPVD
                     EVKVLLNDLKGAGFELGIATGRPYTETVVPFENLGLLPYFEADFIATASDVLEAENMY
                     PQARPLGKPNPFSYIAALYGNNRDKYESYINKQDNIVNKDDVFIVGDSLADLLSAQKI
                     GATFIGTLTGLKGKDAAGELEAHHADYVINHLGELRGVLDNL"
     gene            complement(14571..14666)
                     /locus_tag="POB55_000257"
     CDS             complement(14571..14666)
                     /locus_tag="POB55_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499253.1"
                     /translation="MKPLVQSFTSSCAVKQQSNYKLSSTPLSSPK"
     gene            complement(14760..16364)
                     /locus_tag="POB55_000258"
     CDS             complement(14760..16364)
                     /locus_tag="POB55_000258"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDI6499254.1"
                     /translation="MKQFNVLVADPISKDGIKALLDHEQFNVDIQTGLSEEALIKIIP
                     SYHALIVRSQTTVTENIINAADSLKVIARAGVGVDNININAATLKGILVINAPDGNTI
                     SATEHTLAMLLSMARNIPQAHQSLTNKEWNRNAFKGTELYHKTLGVIGAGRIGLGVAK
                     RAQSFGMKILAFDPYLTDEKAKSLSITKATVDEIAQHSDFVTLHTPLTPKTKGLINAD
                     FFAKAKPSLQIINVARGGIIDEKALIKALDEGQISRAAIDVFEHEPATDSPLVAHDKI
                     IVTPHLGASTVEAQEKVAISVSNEIIEILIDGTVTHAVNAPKMDLSNIDDTVKSFINL
                     SQTVGELAIQLMYNAPSSIKITYGGDLASIDSSLLTRTIITHILKDDLGPEVNIINAL
                     MLLNQQQVTLNIENNKAETGFSNYLEVELSNDSDSVKVGASVFTGFGPRIVRINNFSV
                     DLKPNQYQIVSYHNDTPGMVGKTGALLGKYNINIASMTLGRTEAGGDALMILSVDQPV
                     SNNIIDELKQVGEYNQIFTTELTVQS"
     gene            complement(16351..17511)
                     /locus_tag="POB55_000259"
     CDS             complement(16351..17511)
                     /locus_tag="POB55_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HDI6499255.1"
                     /translation="MYYHQPLLLTPGPTPVPDAIMREIQAPMVGHRSKDFEDIAQQAF
                     QGLKPIFGSQNDVLILTSSGTSVLEASMLNIVNPEDHFVVIVSGAFGNRFKQIAQTYY
                     KNVHIYDVTWGEAVDVKDFINFLSTLNVEVKAVFSQYCETSTTVLHPIHELGNAINQF
                     NSNIYFVVDGVSCIGAVDVDINKDKIDVLVSGSQKAIMLPPGLAFVAYSHRAKERFKE
                     VTTPKFYLDLNKYISSQADNSTPFTPNVSLFRGVNAYVETVKAEGFNHVIARHYAIRN
                     ALRSALKALDLTLLVNDKDASPTVTAFKPNTNDEVKIIKDELKNRFKITIAGGQGHLK
                     GQILRIGHMGKISPFDILSVVSALEIILTEHRKVNYIGKGISKYMEVIHEAI"
     gene            complement(17625..18071)
                     /locus_tag="POB55_000260"
     CDS             complement(17625..18071)
                     /locus_tag="POB55_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HDI6499256.1"
                     /translation="MHQHDFKVQTSWQGGRNNVGNVQGDILSENISIPASLGGVGLGT
                     NPDELLVSAASSCYIISLAATLERAKFTDISIEQQSIGTACLNNGKFSMSKIVHHPQI
                     QIPSDQIAQLEKRLPKLITIADNNCMISNAVRNNVDIKIYPIIQAK"
     gene            18151..18894
                     /locus_tag="POB55_000261"
     CDS             18151..18894
                     /locus_tag="POB55_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDI6499257.1"
                     /translation="MTLNKLKDELQIVSHRGLPSDFPENTMVGYREVMGLNVAMLEID
                     VHLTKDQHFVVIHDETIDRTSDGKGRISDYTLSQLKSFDFGSYKDVAFKGERIPTLDE
                     VLSLCLKYDKKLLIELKSPNLYPEIECKLLAFLEEKKVDATQVVIQSFDIECIEKLNT
                     LGSIYELGVLCSKRNYWYKKPNFSRIAQIASYVNPNYALVTRKFVDKAHHHQLQVMPY
                     TVNKLKTGEKLRQMGVDGLITDNPKLFIK"
     gene            complement(19110..19712)
                     /gene="rpsD"
                     /locus_tag="POB55_000262"
     CDS             complement(19110..19712)
                     /gene="rpsD"
                     /locus_tag="POB55_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019165370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HDI6499258.1"
                     /translation="MARFRGSNWKKSRRLGISLSGTGKELEKRPYAPGQHGPNQRKKL
                     SEYGLQLREKQKLRYLYGMTERQFRNTFDIAGKKFGVHGENFMILLASRLDAVVYSLG
                     LARTRRQARQLVNHGHILVDGKRVDIPSYSVKPGQTISVREKSQKLNIIVESVEINNF
                     VPEYLNFDADSLTGTFVRLPERSELPAEINEQLIVEYYSR"
     gene            complement(19956..20420)
                     /locus_tag="POB55_000263"
     CDS             complement(19956..20420)
                     /locus_tag="POB55_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HDI6499259.1"
                     /translation="MTTINPTNYTLLKKQAASLIEDEHHMIAILSNMSALLNDNLDQI
                     NWVGFYLLEQNELILGPFQGHPACVHIPIGKGVCGTAVSERRTQVVADVHQFEGHIAC
                     DANSKSEIVVPIFKDDKIIGVLDIDAPITDRFDDNDKEHLEAIVKIIEKQLA"
     gene            20557..22251
                     /gene="ezrA"
                     /locus_tag="POB55_000264"
     CDS             20557..22251
                     /gene="ezrA"
                     /locus_tag="POB55_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HDI6499260.1"
                     /translation="MVLYIILAIIVIILIAVGVLFYLRSNKRQIIEKAIERKNEIETL
                     PFDQNLAQLSKLNLKGETKTKYDAMKKDNVESTNKYLAPVEEKIHNAEALLDKFSFNA
                     SQSEIDDANELMDSYEQSYQQQLEDVNEIIALYKDNDELYDKCKVDYREMKRDVLANR
                     HQFGEAASLLETEIEKFEPRLEQYEVLKADGNYVQAHNHIAALNEQMKQLRSYMEEIP
                     ELIRETQKELPGQFQDLKYGCRDLKVEGYDLDHVKVDSTLQSLKTELSFVEPLISRLE
                     LEEANDKLANINDKLDDMYDLIEHEVKAKNDVEETKDIITDNLFKAKDMNYTLQTEIE
                     YVRENYYINESDAQSVRQFENEIQSLISVYDDILKEMSKSAVRYSEVQDNLQYLEDHV
                     TVINDKQEKLQNHLIQLREDEAEAEDNLLRVQSKKEEVYRRLLASNLTSVPERFIIMK
                     NEIDHEVRDVNEQFSERPIHVKQLKDKVSKIVIQMNTFEDEANDVLVNAVYAEKLIQY
                     GNRYRKDYSNVDKSLNEAERLFKNNRYKRAIEIAEQALESVEPGVTKHIEEEVIKQ"
     gene            complement(22325..22465)
                     /locus_tag="POB55_000265"
     CDS             complement(22325..22465)
                     /locus_tag="POB55_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499261.1"
                     /translation="MIMLFSLLILIKTTHLITFIFKQLLFIFIVPFIYPYKKEARQRI
                     VG"
     gene            22614..23762
                     /locus_tag="POB55_000266"
     CDS             22614..23762
                     /locus_tag="POB55_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDI6499262.1"
                     /translation="MFILIYLDNAATTKAFEEVLDTYLKVNQSMYYNPNSPHKAGLQA
                     NQLLQQAKAQINAMINSKTNYDVVFTSGATESNNLALKGIAYRKFDTAKEIITSVLEH
                     PSVLEVVRYLEAHEGFKVKYVDVKKDGSINLEHFKELMSDKVGLVTCMYVNNVTGQIQ
                     PIPQMAKVIKNYPKAHFHVDAVQAFGKISMDLNNIDSISLSGHKFNGLKGQGVLLVNH
                     IQNVEPTVHGGGQEYGVRSGTVNLPNDIAMVKAMKIANENFEALNAFVTVLNNDVRQF
                     LNKYHGVYINSSTSGSPFVLNISFPGVKGEVLVNAFSKYDIMISTTSACSSKRNKLNE
                     VLAAMGLSDKSIEGSIRLSFGATTTKEDIARFKEIFIIIYEEIKELLK"
     gene            23762..24985
                     /gene="thiI"
                     /locus_tag="POB55_000267"
     CDS             23762..24985
                     /gene="thiI"
                     /locus_tag="POB55_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HDI6499263.1"
                     /translation="MKYDHLLVRYGELTLKGSNRKKFVNQLRNNVNKSLKGLDGFVVK
                     GKRDRMYIELEDHADINEITYRLSKIFGIKSISPVLKVEKTIEAMSAAAIKFAQQFEE
                     NSTFKIDVKRADKNFPMDTYELQRELGGAILKHFDNISVNVKRPDHEIRVEVRLDAIY
                     MYEEVVPGSGGLPVGTGGKTLLMLSGGIDSPVAGMEVMRRGVTIEAIHFHSPPFTSDQ
                     AKEKVIELTRILAERVGPIKLHIVPFTELQKQVNKVVHPRYTMTSTRRMMMRVADKLV
                     HQIGALAIVNGENLGQVASQTLHSMYAINNVTSTPVLRPLLTYDKEEIIIKSKEIGTF
                     ETSIQPFEDCCTIFTPKNPVTEPNFDKVVQYESVFDFEEMINRAVENIETLEITSDYK
                     TIKEQQTNQLINDFL"
     gene            25029..25799
                     /locus_tag="POB55_000268"
     CDS             25029..25799
                     /locus_tag="POB55_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HDI6499264.1"
                     /translation="MDLNLTMIIIIILFGFIAAFIDSVVGGGGLISTPALLAIGLPPS
                     VALGTNKLASSFGSLTSTIKFIRSGKVDLYVVAKLFGFVFLASACGAYIATMVPSQIL
                     KPLIIIALSSVFIFTLLKKDWGNTRTFTQFTFKKAILFAALFILIGFYDGFVGGGTGS
                     FMLFVLLVFGFDFLSAAGNAKVLNFASNIGALVLFMVLGQVDYVIGLIMATSMIAGSY
                     TGAHFAIKQGVGYVKVLFIIITAILILKNAFDYIQQFV"
     gene            25897..26391
                     /gene="tpx"
                     /locus_tag="POB55_000269"
     CDS             25897..26391
                     /gene="tpx"
                     /locus_tag="POB55_000269"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HDI6499265.1"
                     /translation="MTEITFKGGPIHLKGQQINEGDFAPDFTVLDNDLNQVTLADYAG
                     KKKLISVVPSIDTGVCDQQTRKFNSDASKEEGIVLTISADLPFAQKRWCASAGLDNVI
                     TLSDHRDLSFGENYGVVMEELRLLARAVFVLDADNKVVYKEIVSEGTDFPDFDAALAA
                     YKNI"
     gene            26514..27461
                     /locus_tag="POB55_000270"
     CDS             26514..27461
                     /locus_tag="POB55_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDI6499266.1"
                     /translation="MAEQQTIMERLFHTLDEKAKTLNNENGQSFIENLGLAMEQVYTN
                     ERGLLEQSTLQDRRKAFQFAYLSLMQEEKIQANHQITPDSIGLILGFLVERFMNNQEE
                     LHIVDIASGAGHLSATVKEVLPEIAVMHHLIEVDPVLSRVSVHLANFLEIPFDVYPQD
                     AIMPLPLEEADIVIGDFPVGYYPIDERSKEFKLGFEEGHSYSHYLLIEQAINALKDAG
                     YAFLVVPSNIFTGEHVKQLEKYIATETEMQAFLNLPPTLFKNEKARKSILILQKKKSG
                     ETKPVEVLLANIPDFKIPSQFQGFMTELNQWMDTNRPKK"
     gene            27549..28751
                     /locus_tag="POB55_000271"
     CDS             27549..28751
                     /locus_tag="POB55_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HDI6499267.1"
                     /translation="MSKLILAINAGSSSLKFQLIRMPEEELVTKGLVERIGLKDSIFT
                     IEVNGEKVKTVQDIKDHVEAVDIMLDAFKAHNIINDINDIDGTGHRVVHGGEKFPESV
                     AITDEVEKEIEELSELAPLHNPANLMGIRAFRKLLPNIPHVAIFDTAFHQTMPEKAYL
                     YSLPYHYYKDYGIRKYGFHGTSHKFVSQRAAEMLDKPIEDLRIISCHIGNGASIAAID
                     GGKSIDTSMGFTPLAGVTMGTRSGNIDPALIPFIMEKTGKTAEQVLEILNKESGLLGL
                     SGTSSDLRDLSEEAESGKARSQMALDVFASKIHKYIGSYAARMHGVDVIVFTAGIGEN
                     SVEIRAKVLEGLEFMGVYWDPKKNENLLRGKEGFINYPHSPVKVVVIPTDEESMIARD
                     VMTFGGLK"
     gene            complement(28997..29497)
                     /locus_tag="POB55_000272"
     CDS             complement(28997..29497)
                     /locus_tag="POB55_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDI6499268.1"
                     /translation="MITYKNILIAVDGSHEAEWAFNRAVGVAKRNDAKLTIVNVIDSR
                     TYSSYEVYDAQFTEKSKHFAEELLNGYKEVATNAGVKDVETRLEFGSPKSIIPKKLAH
                     EINADLIMSGTSGLNAVERFIVGSVSESIVRHAPCDVLVVRTEELPADFQPQVATTQL
                     REKYQN"
     gene            29638..30756
                     /gene="ald"
                     /locus_tag="POB55_000273"
     CDS             29638..30756
                     /gene="ald"
                     /locus_tag="POB55_000273"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HDI6499269.1"
                     /translation="MKIGIPREIKNNENRVGLSPSGVHALVESGHTVLVETNAGSGSF
                     FEDVDYKEAGAEIVAEQAKVWDVDMVIKVKEPLESEYPYFKEGLVLFTYLHLANEEKL
                     TQALIDRKVISIAYETVQLPDRSLPLLSPMSEVAGRMSAQVGAEFLQKLNGGMGILLG
                     GVPGVPKGKVTIIGGGQAGTNAAKIALGLGADVTILDVNPKRLQQLDDLFGGRVHTIM
                     SNPLNIELYVKQSDLVIGAVLIPGAKAPRLVTEDMIKQMKNGSVIIDIAIDQGGIFET
                     TDKITTHDDPTYIKHGVVHYAVANMPGAVPRTSTLALNNATLPYALMLANKGYREAFK
                     SNQPLSLGLNTYKGHVTNKGVAEAFEMEYKSVEEALQL"
     gene            30880..30978
                     /locus_tag="POB55_000274"
     CDS             30880..30978
                     /locus_tag="POB55_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499270.1"
                     /translation="MNDIDLIHYMLLMNIPAYVFLACLLHIAFRLG"
     gene            complement(31072..32127)
                     /locus_tag="POB55_000275"
     CDS             complement(31072..32127)
                     /locus_tag="POB55_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDI6499271.1"
                     /translation="MTKISKIIDELNNQQADAAWITTPLNVYYFTGYRSEPHERLFAL
                     LIKKDGKQVLFCPKMEVEEVKASPFTGEIVGYLDTENPFSLYPQTINKLLIESEHLTV
                     ARQKQLISGFNVNSFGDVDLTIKQLRNIKSEDEISKIRKAAELADKCIEIGVSYLKEG
                     VTEREVVNHIEQTIKQYGVNEMSFDTMVLFGDHAASPHGTPGDRRLKSNEYVLFDLGV
                     IYEHYCSDMTRTIKFGEPSKEAQEIYNIVLEAETSAIQAIKPGIPLKDIDHIARNIIS
                     EKGYGEYFPHRLGHGLGLQEHEYQDVSSTNSNLLEAGMVITIEPGIYVPGVAGVRIED
                     DILVTNEGYEVLTHYEK"
     gene            32302..32991
                     /locus_tag="POB55_000276"
     CDS             32302..32991
                     /locus_tag="POB55_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDI6499272.1"
                     /translation="MKLSFHGQSTIYLEGNNKKVIVDPFISNNPKCDLNIETVQVDYI
                     VLTHGHFDHFGDVVELAKKTGATVIGSAEMADYLSSYHGVENVHGMNIGGKANFDFGS
                     VKFVQAFHSSSFTHENGIPVYLGMPMGIVFEVEGKTIYHTGDTGLFSDMSLIAKRHPV
                     DVCFVPIGDNFTMGIDDASYAINEFIKPKISVPIHYDTFPLIEQDPQQFKDAVNVGDV
                     QILKPGESVQF"
     gene            complement(33296..>33704)
                     /locus_tag="POB55_000277"
     CDS             complement(33296..>33704)
                     /locus_tag="POB55_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDI6499273.1"
                     /translation="KNILLGVDTQLKNEKALKEVSKLAGEGTVVTVLNAISEQDAQAS
                     IKAGVHLNKLTEERSKRLEKTRKALEDYGIDYDQIIVRGNAKEELLKHANSGKYEIVV
                     LSNRKAEDKKKFVLGSVSHKVAKRATIPVLIVK"
BASE COUNT        11591 a         4906 c         6192 g        11015 t
ORIGIN      
        1 gaaagcgttt tcacataaca aagggggagt ttcaaatgaa agtcgaagtt tataaaggag
       61 cgcaaggtaa acataacctt aaagattatg aagaaatata taatactttt gattggaaag
      121 acgtagaaca agcattttct tggagtgaaa ctggaaaaat gaacatggca tatgaatgta
      181 tagatcggca tgtagatcaa ggattagggg ataaaatagc gttaaattac aaagatgagt
      241 acagaaaaga atcgtatact tataaagata tgcaacggtt atctaataaa gcagcgaatg
      301 ttttgtctga acatgcagaa gttgacaaag gtgacagagt atttatattt atgtcgcgta
      361 cacctgaact atattttgcg ttgttaggtg ttttaaaaat tggtgcaatt gttgggccgt
      421 tatttgaagc atttatggaa aaggcagttg cggatagatt agagaacagt gaagctaaag
      481 tgttaattac taataaggca ttgttacctc gagtacctgt agataaatta ccgaacttga
      541 aaaaaattgt tgtcgtagat gaggatgtag aagacaatta catagacttc attagtttga
      601 tggaaactgc tagcgatgaa tttgacattg aatggttaaa gtcggatgat ggtttgattt
      661 tacattatac atcaggttct actgggcaac ctaaaggtgt attgcatgtt caacaagcaa
      721 tgttagtgca ctatatttct ggaaaatatg tattagattt acaagaagat gatgtttatt
      781 ggtgtacagc agatccaggt tgggttacag gaacatctta tggtattttt gcaccatggt
      841 taaatggcgc tacaaattgt atagctggtg gtcgcttttc accagaacag tggtatagta
      901 tgattgaaga ttttaaagtg acgatttggt atacggcacc aacagcttta agaatgttaa
      961 tgagtgctgg tgacgatatt gttgagaaat atgacttgtc atcgttacgt tcgattctat
     1021 cagtaggtga gcctttaaat cctgaagtta taaaatgggc gaaaaaagta tacggtttaa
     1081 cggtgttaga tacttggtgg atgacagaaa caggtggaca tatgattgtt aactatccaa
     1141 cgatggacgt caagcttggc tcaatgggca aaccattacc tggtattcaa gctgcaatta
     1201 tcgatgatgc aggtaatgaa ttaccaccaa atcgaatggg caaccttgct ataaaaaaag
     1261 gctggccatc aatgatgtat cgtatctgga agaatccaga aaaatataag tcatatttta
     1321 ttggagactg gtatgtatct ggtgattcgg catataaaga tgaagatggt tacttctggt
     1381 tccaaggacg tgttgatgat gtaattatga cagctggtga acgagttgga ccatttgagg
     1441 ttgagtctaa attggttgaa cacgaagcag ttgccgaagc aggaattatt ggtaaacctg
     1501 atccggttcg cggtgaaata attaaggcgt ttgttgcact gagaaaagga tatgaaccaa
     1561 cagacgaatt aaaagaagaa attcgtttat ttgttaaaga aggtttgtcg gcacatgcag
     1621 caccacgtga aatcgaattt aaagataaat tacctaaaac acgctcaggt aaaattatga
     1681 gacgtgtatt aaaagcttgg gaattaaatt tagatgcagg ggatttaagt acaatggaat
     1741 aatgacatga atgttattga agatttcttt cgaagaataa agggatacaa catatttcat
     1801 gttaatgttt aaatattagt ttactttatg ataagcaata taaagagctg ttaacttgtg
     1861 tcatatcatt tcgtagaaag catttgaaaa tgatgacata acaataatgg catatcttta
     1921 tattgctttt tatttttaat atgatctttg gaatatgact attttaaata atagaaaaat
     1981 atagttatca atagtatcaa gcgctaaaag ttgtataata caaaacttta ataagtgaat
     2041 ttattgcaaa aatgaaagcg ctaacccgat ttagtcgaca agtttttaac agttcgttat
     2101 tatatgaatg taagtaaaaa tttcttagct acaacttaca tattataaat gcataaatta
     2161 aacaaaaagg ggcgaaaaaa gttgactcat ttatcagatt tagatattgc gaatcaatca
     2221 acactacaac caattaagga tattgctgca tcagtaggta tttcagagga tgcattagaa
     2281 ccttatggtc attacaaagc taaaatcgac attaataaaa ttacgccaag agaaaacaaa
     2341 gggaaagttg ttttagtaac tgcgatgagc ccaacaccag ctggtgaagg taaatcaacg
     2401 gttacagttg gtttagctga tgcattccat gagttaaata aaaacgttat ggttgcatta
     2461 agagagcctg ctttaggacc aacatttggt attaaaggtg gtgcgactgg tggtggttat
     2521 gcgcaagtct tacctatgga agatatcaac ttacatttta atggggattt ccatgcgatt
     2581 acaactgcta ataatgcatt gtctgcgttt atagataatc atattcacca aggtaatgaa
     2641 ttaggaatcg atcaaagacg tattgaatgg aaacgtgtat tagatatgaa tgatcgtgca
     2701 cttagacatg taaatgttgg gttaggtgga cctacaaatg gtgtaccacg tgaagatggc
     2761 tttaatatta cagtagcgtc tgaaattatg gcgattttat gtttaagtag aagtattaaa
     2821 gacttaaaag ataaaattag tcgtattact attggttata ctagagatcg caagccagtt
     2881 acagttgcag atttaaaagt gcaaggtgca cttgcaatga ttttaaaaga tgcaattaaa
     2941 ccaaacttag tacaatcaat tgaagggaca ccagcattag ttcatggtgg accatttgcg
     3001 aatatcgcac acggttgtaa ctcaatttta gcaactgaaa cagcacgtga tttagctgat
     3061 atcgttgtaa cggaagctgg atttggttca gacttaggcg ctgaaaaatt catggacatt
     3121 aaagcgcgtg aagcaggatt tgatccggca gctgtcgttg ttgttgcgac aattcgtgcg
     3181 ttaaaaatgc atggtggtgt agcgaaagat aatttaaaag aagaaaatgt agaagcagta
     3241 aaagcaggaa ttgttaattt agagcgtcat gttaataata ttaaaaaatt cggtgtagaa
     3301 ccggttgttg caattaatgc atttatacat gataccgatg cagaagtaga atatgtaaaa
     3361 tcttgggcta aagaaaataa cgtacgaatt gccttaactg aagtttggga aaaaggtggt
     3421 aaaggtggcg ttgacttagc aaatgaagta ttagaagtca ttgatcaacc taattcattt
     3481 aaacctttat atgaattaga attaccatta gagcaaaaga ttgaaaagat tgtgactgaa
     3541 atctatggcg gttcaaaagt aacgtttagc agtaaagcgc aaaaacaatt aaaacaattt
     3601 aaagaaaatg gttgggataa ttacccagta tgtatggcga aaacacaata ttcattctca
     3661 gatgatcaaa cgttgttagg tgcaccatca ggatttgaaa ttacaattcg tgaattagaa
     3721 gcgaaaacag gtgcaggatt tatcgtagcg ttgacaggtg caatcatgac tatgcctggt
     3781 ttacctaaaa aaccagcagc attaaacatg gatgttactg atgatggtca tgcaattggg
     3841 ttattctaat aaatcatgtc aattgtttaa taaagataag taaatagttt aatagaccgg
     3901 actgttggag atgcattatt tcagcagttc ggttttttgc tgtgctaaaa atagattcaa
     3961 tttggcgaat ctaacgacaa tgtttgaagg tggttaatta atgtatatga agataaaaag
     4021 tgggcttgaa gaataggaaa gcgatgcaat gaatattcca tattaaaaaa aaataataaa
     4081 ataggttgca atatttaatt gggatgcgct acaattaaca ctaataattg atattgataa
     4141 ttattatcaa ttaaatataa tcttatagga gttgttaaca acatgaacaa acatcaccca
     4201 aaattaaggt ctttctattc tattagaaaa tcaattctag gcgttgcatc ggtcattgtc
     4261 agtacactat ttttaattac ttctcaacat caagcacaag cagcagaaaa tacaaatact
     4321 tcagataaaa tctcggaaaa tcaaaataat aatgcaacta caactcagcc accaaaggat
     4381 acaaatcaaa cacaacctgc tacgcaacca gcaaacactg cgaaaaccta tcctgcagcg
     4441 gatgaatcac ttaaagatgc aattaaagat cctgcattag aaaataaaga acatgatata
     4501 ggtccaagag aacaagtcaa tttccaatta ttagataaaa acaatgaaac gcaatactat
     4561 cactttttca gcatcaaaga tccagcagat gtgtattaca ctaaaaagaa agcagaagtt
     4621 gaattagaca tcaatactgc ttcaacatgg aaaaagtttg aagtctatga aaacaatcaa
     4681 aaattaccag tgagacttgt atcatatagt cctgtaccag aagaccatgc ctatattcga
     4741 ttcccagttt cagatggcac acaagaattg aaaattgttt cttcgactca aattgatgat
     4801 ggagaagaaa caaattatga ttatactaaa ttagtatttg ctaaacctat ttataacgat
     4861 ccttcacttg tgaaatcaga tacaaatgat gcagtagtaa cgaatgatca atcaagttca
     4921 gacgcaagta atcaaacaaa cacgaataca tctaatcaaa atacatcaac gatcaacaat
     4981 gctaataatc aaccgcaggc aacgacaaat atgagtcaac ctgcacaacc aaaatcgtca
     5041 gcgaatgcag atcaagcgtc aagccaacca gctcatgaaa caaattctaa tggtaatact
     5101 aacgataaaa cgaatgagtc aagtaatcag tcggatgtta atcaacagta tccaccagca
     5161 gatgaatcac tacaagatgc aattaaaaac ccggctatca tcgataaaga acatacagct
     5221 gataattggc gaccaattga ttttcaaatg aaaaatgata aaggtgaaag acagttctat
     5281 cattatgcta gtactgttga accagcaact gtcattttta caaaaacagg accaataatt
     5341 gaattaggtt taaagactgc ttcaacatgg aagaaatttg aagtttatga aggtgacaaa
     5401 aagttaccag tcgaattagt atcatatgat tctgataaag attatgccta tattcgtttc
     5461 ccagtatcta atggtacgag agaagttaaa attgtgtcat ctattgaata tggtgagaac
     5521 atccatgaag actatgatta tacgctaatg gtctttgcac agcctattac taataaccca
     5581 gacgactatg tggatgagga aacatacaat ttacaaaaat tattagctcc gtatcacaaa
     5641 gctaaaacgt tagaaagaca agtttatgaa ttagaaaaat tacaagagaa attgccggaa
     5701 aaatataagg cggaatataa aaagaaatta gatcaaacta gagtagagtt agctgatcag
     5761 gttaaatcag cagtgacgga atttgaaaat gttacaccta caaatgatca attaacagat
     5821 gtacaagaag cgcattttgt tgtttttgaa agtgaagaaa atagtgagtc agttatggac
     5881 ggctttgttg aacatccatt ctatacagca actttaaatg gtcaaaaata tgtagtgatg
     5941 aaaacaaagg atgacagtta ctggaaagat ttaattgtag aaggtaaacg tgtcactact
     6001 gtttctaaag atcctaaaaa taattctaga acgttgattt tcccatatat acctgacaaa
     6061 gcagtttaca atgcgattgt taaagtcgtt gtggcaaaca ttggttatga aggtcaatat
     6121 catgtcagaa ttataaatca ggatatcaat acaaaagatg atgatacatc acaaaataac
     6181 acgagtgaac cgctaaatgt acaaacagga caagaaggta aggttgctga tacagatgta
     6241 gctgaaaata gcagcactgc aacaaatcct aaagatgcgt ctgataaagc agatgtgata
     6301 gaaccagatt ctgacgtggt taaagatgct gataataata ttgataaaga tgtgcaacat
     6361 gatgttgatc atttatccga tatgtcggat aataatcact tcgataagta tgatttaaaa
     6421 gaaatggata ctcaaattgc caaagatact gatagaaatg tggataaagg tgccgataat
     6481 agcgttggta tgtcatcgaa tgtcgatact gataaagact ctaataaaaa taaagacaaa
     6541 gtcatacagc tgaatcatat tgccgataaa aataatcata atggaaaagc agcaaagctt
     6601 gacgtagtga aacaaaatta taataataca gacaaagtta ctgacaaaaa aacaactgaa
     6661 catctgccga gtgatattca taaaactgta gataaaacag tgaaaacaaa agaaaaagcc
     6721 ggcacaccat cgaaagaaaa caaacttagt caatctaaaa tgctaccaaa aactggagaa
     6781 acaacttcaa gccaatcatg gtggggctta tatgcgttat taggtatgtt agctttattc
     6841 attcctaaat tcagaaaaga atctaaataa ttaactggat atagcatatg tatgattaac
     6901 tttgtagaca atgtgaaacc aattaaatta taaactattg attggtttaa tggctttcct
     6961 ttagagtaaa taaaaagaac agcagtgaga aattttctaa ttgaaaataa tcttactgct
     7021 gtttttaata tttggattca ttgttgtggt tactttaaaa agtgagcatc aattaacgct
     7081 tttttcgatt taacaaatgt gatttaatat catattttaa tgcgtcgttg tattcttttt
     7141 cagtgatttg atcttcgatt aacatacgct ttaatacata atgttgtctt tggatactat
     7201 atttcaaatc tttatccgat tttaatgttc catctttttc gtagggtgta tagccataag
     7261 ggctttgcaa caaaccgata aggtatgcag attgtgcaat tgataaatct tttggtggaa
     7321 taccaaacaa actatatgaa gcggatgcaa ttccggaaat attagcgcca ttataatctc
     7381 taccgaaggg aactatattt aaatatgtat atataatttc atcttttgag agtaggtgtt
     7441 ctaatctaat tgctaggcga agttcatttg cttttctact atatgttttt tcgttggtaa
     7501 gaacttgatt tttaacaagt tgttgtgtaa ttgtgctacc acctgaactt tgatcagtat
     7561 taaaaatatc ttgtatcatt gctcttaaaa tcgcctttgg taagatgcca tcatgtttat
     7621 aaaataaagt gtcttcagat gacgttaatg ctttaatgac atttggactt gatgttttag
     7681 ggcctataat gagtgagttt tgagaatggt tatactcata taataaattt ttgttattat
     7741 gatctaataa ttcatcgcca ggtatttgtc gaactttttt tattaaagca tcatctgata
     7801 atgagtcgga cgttttagtt aaatgatgaa aatataaaga catcgcaatc acagcgatag
     7861 caacgatggc aatgaaaatc ataaatataa aaacaatgac atgtttgata gctttatata
     7921 tcttttcaaa atgatatata cgatgattca attgatgatt gttgtcttga ttcgtcatct
     7981 ttaacctcct aaattacgta tagtatacca tatttaagca atttgacttt tattttaacg
     8041 ttaattataa ttagggaaaa tgcataaaga ataggataaa aggacaagta atattatttg
     8101 aaatcatcag agagctagtg gtcgctgtga actagtgttt ttaataatat gaatacacct
     8161 ttataactaa catgtcctac gtttattaat gaacgtatca ttaatagagt gaacattatc
     8221 ttatgattat gtttaagttt ggtggtaacg tgcaatcgcg cccttacata tttgttgtaa
     8281 gggttttttt tacgttcaaa aaactttaaa gtattttaac aagtattcaa taaataatta
     8341 taaataggag gaaataataa tgacgaatgt attaattgaa gatttaaaat ggagaggtct
     8401 tatttatcaa caaactgatg aacaaggtat tgaagattta ttaaataaag aacaagtgac
     8461 gttatactgc ggtgccgatc caacggcaga tagtttacat attggtcact tactaccatt
     8521 cttaacatta agacgttttc aagaacatgg acatcgtcct atcgttttaa ttggcggtgg
     8581 tacaggtatg attggtgatc catcaggtaa atcagaagaa cgtgtgctac aaacagaaga
     8641 acaagtagat aaaaatatcg aaggtattag taagcaaatg cacaatattt ttgaatttgg
     8701 aacagaccat ggtgcagtgc ttgttaataa tagagactgg ttaggacaaa tctcattaat
     8761 tagcttttta cgtgactatg gtaaacacgt cggcgttaat tacatgttag gtaaagattc
     8821 aatccaaagt cgtttagaac atggtatttc atatacagaa ttcacataca cgattttaca
     8881 agctattgat ttcggtcatt tgaatagaga attgaattgt aagattcaag taggtggatc
     8941 agatcaatgg ggtaatatca caagtggtat tgaattaatg cgtcgtatgt atggtcaaac
     9001 agacgcatac ggtttaacta ttccgcttgt aactaaatca gatggtaaga aatttggtaa
     9061 gtctgagtca ggtgctgttt ggttagatgc tgaaaaaaca agtccttatg aattttatca
     9121 attctggatt aatcaatcag acgaagatgt aattaaattc ttaaaatact ttactttctt
     9181 aggaaaagaa gaaattgatc gcttagaaca atctaaaaat gaagcaccgc atttacgtga
     9241 agctcaaaaa acattagctg aagaagtaac taaatttatt catggtgaag atgcattaaa
     9301 tgatgcaatc cgtatttcac aagcattatt tagtggtgat ttaaaatcat tatcagcgaa
     9361 agaattaaaa gatggattta aagatgtgcc tcaagtgaca ttatcaaatg acacaacaaa
     9421 tatcgttgaa gtccttattg aaacaggcat ttctccttct aaacgacaag cacgtgaaga
     9481 tgttaacaat ggtgcgattt atattaatgg tgagagacaa caagatgtta attatgcttt
     9541 agcaccagaa gataaaattg atggcgaatt tacgattatt cgtcgcggta agaaaaaata
     9601 cttcatggtt aactatcaat aaatataatt gcatagctaa ataaattaga gcctactcat
     9661 attcattcct aagaatgtaa tgagtaggct cttaatgtac ttttctgtct gtaaattatc
     9721 taaagaaatc tctatcgcct tggccaggtg attgacgttc tgatcggctt tgacgttttg
     9781 gttgttcttt ttgttgtttt aatttcactt taacttcttt tgttttacca tcacggataa
     9841 cggtaacagt gactgattca ccaggttttt tattttcata taaatagctt cttaaatcaa
     9901 catcatcttt aattttcttg ccatcaattt ctgtaataat atcacctttt ttaagatcaa
     9961 tatcactatc agctttggcg acataaatac cgtcttctct atcagtatga agttgctcgc
    10021 gctcttcttc aggaatatct ttcaaattaa ttaaaccaat accaatcgaa gggcggtcaa
    10081 ttttaccatg ttttacaagt tgttcaattg ttactttaac ttcattactt ggaatagcaa
    10141 acccgatacc ttcaacttgt gtcgcagcaa ttttcattga gttaatacca actaaattac
    10201 cattaatatc tactaatgcg ccacctgagt tacctgggtt aatagcagca tctgtttgaa
    10261 gaacgctgac ttttgtattg ccaccagttg tctcagcgtc aatagtacgt tcgcttgctg
    10321 aaatgatacc agatgttaca gagttagcaa attgtaatcc taatgggtta cccattgcga
    10381 atacgctatc gccagtttgt acttttgaag agttggcgaa ttgaatcgct ttaatacctt
    10441 ttgtattttc aattttaagt acagcaatat cagttactgc atctttacca actaatttcg
    10501 ctttaacttg ttttttatta tgtaattgga ctctaatttc atttgcgcca tcaataacat
    10561 gattgtttgt aacgatataa gctgaattgt tgtttatttg atagataaca cctgaaccta
    10621 ctccagcttc agatggttta gatgatttgc cttttaataa gtcgtctaca cttgatgctt
    10681 tttgcatgtt aataactcca acaattgtag gggagacaga ttttatcatt tcatgaacgg
    10741 taccgaattt cttgctttga ccgtctaatt gattgccacc tttattattt gttgtctgaa
    10801 cagttgaacc atctttattt aaaattgtac tatttaatac tttgcctata ccaagtacta
    10861 gaagtgcacc aataattcca gcaatcaatg caacgatgac tgttttaaac catggaaatt
    10921 taggtcttct gtatctaggt gtttggctat ggtttgttgt agaatgatct gtatgattaa
    10981 aatctgacat acttaacctc cattatatga tttatatatg ctttaattat gtcttttttt
    11041 tataattatt gctagtaata cactatacaa ctttagatgt attttcagac tatcttgttc
    11101 tagtaaaaat atagtgataa aatctagttg ttataaacaa ataagttaag taggtggcac
    11161 aatgtattca gtgattagta agattttgaa tttcatttta gttaaaatgt caaaatcatt
    11221 atacgtaatc ggtaaagata atattcctaa ggatagtaaa tatgtcgtaa cttgtacgca
    11281 tgaaagttat aacgaagtca ttatgctagg tatggcatta tatcctaatc aaattcatta
    11341 tatggctaaa aaagaattat tcaaaaataa atggattggt aaatttttaa cgtctttaaa
    11401 tgcatttcct gtagatcgtg aaaatccagg acctagtact ttaaaacgcc caatcaattt
    11461 gttgaaagat aataagacag ttggtatctt cccaacaggt tctcgcacat cgcaagaagg
    11521 tgcacctttg aaacgtggtg cttcaacaat tgcaatgtta agtaagtcac caatattacc
    11581 tgttgcttat gttggtccta ctaagattca cggtctttta acaggacaag catacataaa
    11641 tattggtaaa ccaatcgata ttaacgattt acctaaagat ttaaaacgaa acgagcgtat
    11701 cgattatatt acaaaagaga ttgaaacacg aacagcaaag ttacaacaag aattgcatga
    11761 aattgtaaaa agtttataaa ataacttgag gtagaaatat agctggttag taagcctagg
    11821 tgactataac ggagtatcga agtagttatt ctagttacat tagtaatctt tatatttcta
    11881 ccttatttta agaaggatga cattcaatgt ataaattttt ccaaaattta gggcgttcgt
    11941 taatgttacc agttgccatt ttaccggctg cagcaattat tgccggaatt ggtaatacgt
    12001 taaatgcact tcatgcgacg ccgaaaatcg caatgttctt tactacggta ggtactacga
    12061 ttttagagca attaggtatt ttatttgcaa ttggtgtagc tataggaatg gcaaagaaaa
    12121 atgatggtgc agtcgcgctt gcagctacgt taggatattt tttagttaca gttgtattat
    12181 caccaatgaa attagcgcct ttattaggaa tgaaagcttc ggaaataaat tcagcttttg
    12241 aaaagatgaa taatggcaat gtctttgttg gtattgtaat aggtttaata gcagcttatg
    12301 catacaataa gtttagtgaa atagaattac cattagcatt atcatttttt agtggtaaac
    12361 gtctagttcc aattatgact gcattttact gtacattttt agttgtcata ttgttattct
    12421 tatggccact actttattca tggattgtaa aatttggtga atctatagtt ggtttaggat
    12481 cgtttggtgc tttcatatat ggtgttgcta acagactatt aattcctaca ggattacatc
    12541 atgcactgaa tagtgtgttc tggtttgata cgattggtat taatgatatt ggtaaattcc
    12601 aaagtggcaa agatgctata aaaggtatta caggacgcta tcaagcggga ttcttcccaa
    12661 tcatgatgtt cggtattcca gcggctgcgt tagcaatgta tcatacagct aaaacgactc
    12721 agaaaaaaca agtttacggt tggttcctag cgagttcagt tgcagcattt ttcgttggtg
    12781 ttacagagcc gattgagttt gcatttatgt ttgttgcgcc aatcttatat gttgttcatg
    12841 cgttattaac aggattatct ttatttatcg cagctacatt ccattggaca gctggatttt
    12901 catttagtgc aggtttgata gattatgtgt tatcactaat taatccggta tcaaatcatc
    12961 cattgatgtt gcttgttcaa ggtgttgtat ttttcatctt atactatgtg attttccgtg
    13021 tagtaatcca agtatttaac ttgaatacga ttggtagagg tgaaaatgaa ttagttgatc
    13081 caacagttgt aaaagataat attgctcctg gtgaaaatga tattaaacaa agtaaatatc
    13141 atcaacatgc tatacaaata ttagaaggtt taggtggtca agagaatatt gttaatttaa
    13201 ctaattgtgc aacaaggttg cgtctagagt taaaagacac atctattatt gatcaacaga
    13261 agattaaaaa cgccggtgca gttggtgtta ctgttaatgg taaacattca actcaagtta
    13321 ttgtcggtac acacgtacag caggttgctg atgaaattga aaaacattta taaaatttaa
    13381 acagctaaag cgcaaatcca acttcaatag ggtttgcgct ttttttagtc aaatagtaga
    13441 atgatttata tctgaatttt cagaaatgga gtggggattg tgaagaaaat tttatttgat
    13501 gttgatggcg tttttttaag tgaagaacgt tgtttcgatg tctctgcatt aacggtatat
    13561 gaattattaa tggataagtg ttatttaggt cttcattcac atatagattg ggaaactttg
    13621 actgataacg atatacaaga cattaggaat cgaatttttc aaaaagataa gattttaaac
    13681 aaattaaaat cgctagggct aaattcaaat tgggatatgt tatttatcgt ttttagtatt
    13741 catttaattg atattttgaa aaagttatca catgatgaaa ttgaggcatt catgtatcaa
    13801 gatgagcctg tcgaattgaa gttgcaaaat ataagcacaa acttagcaga ctgttttaat
    13861 ttaaatgaac aactaccttt acaatttttg gataatgtaa aagttggtaa aaataatatt
    13921 tatgctgctt tggaagagtt tgcaacaaca gagttacatg tttcggatgc tactttattt
    13981 agtttgaagg gtgcattatg gacgttagcg caagaagttt atcaagaatg gtatttagga
    14041 tcgaagttgt atgaagatgt tgaaaagaaa atagcacgaa ctacttttaa gacaggttat
    14101 atttatcaag aaattatttt gagaccagta gatgaagtta aggtacttct gaatgattta
    14161 aaaggtgctg gtttcgaatt aggtattgca acaggtcgtc cttatactga gactgttgtg
    14221 ccatttgaaa atttaggatt gttaccatat tttgaagctg attttattgc aacagcaagt
    14281 gatgttttag aagcagagaa tatgtatccg caagcacgac cattaggaaa gccgaatcct
    14341 tttagttata tcgcagcttt atatggtaat aatcgcgata aatatgaatc ttatatcaat
    14401 aagcaagata acattgtaaa taaagatgac gtatttatag taggcgattc gttagctgac
    14461 ttattaagtg ctcaaaaaat aggtgcaacg tttattggaa cattaacagg tttaaaaggt
    14521 aaggatgctg caggtgagtt agaagcgcat catgccgact atgttattaa tcatttaggt
    14581 gaacttagag gtgtactaga taatttgtaa tttgattgtt gtttgacagc acaacttgaa
    14641 gtgaatgatt gaaccaaagg tttcatattg agttacaatg aaattaataa tgaaaaaatg
    14701 ccaagaagca atggaagtaa tccaatgtct tcttggcatt ttgaatttac ataaattgtt
    14761 tatgactgta ccgtcaattc agttgtgaaa atttgattgt attcaccaac ttgtttaagt
    14821 tcatcaatta tattgtttga aacaggttga tcaacggata aaatcattag cgcatctccg
    14881 cccgcttcag ttctacctaa agtcatagat gcaatgttga tattgtattt acctaacaat
    14941 gcgccagttt ttcctaccat acctggagta tcattatgat atgacacaat ttgatattga
    15001 tttggcttta agtctacaga aaaattatta attctaacaa ttcttggacc gaaacctgta
    15061 aagacagaag cgccaacttt aacggaatcg ctatcgtttg atagttctac ctctaagtag
    15121 ttactaaaac ctgtctctgc tttattattt tcaatattta atgtcacttg ttgttgattt
    15181 aacaacatta gagcattgat aatattgact tcaggaccaa gatcatcttt taaaatatga
    15241 gtaataattg tacgtgttaa taaactacta tcaatagagg ctaagtcgcc accgtacgta
    15301 attttaatag agcttggtgc attgtacatt aattgaatag ctaattcacc aactgtttgg
    15361 cttaaattga tgaatgattt tacagtatca tctatattgc ttaagtccat tttaggtgca
    15421 ttcactgcat gcgttacagt accatcaatt aaaatttcga tgatttcatt tgaaacagaa
    15481 attgccactt tttcttgagc ttcgactgtt gaagcaccca aatgaggtgt aacaataatt
    15541 ttatcatgtg caacaagagg cgagtcagtt gcaggttcat gttcaaacac atcgatagct
    15601 gcccgactaa tttgtccttc gtctaatgct tttattagcg ccttttcatc aataatacca
    15661 ccacgtgcca cattgattat ttgcaaacta ggttttgctt tggcaaaaaa gtcagcatta
    15721 attaagcctt ttgttttagg tgttagtggt gtatgtaatg taacgaaatc agaatgttgg
    15781 gcaatctcat caactgttgc cttcgtaatg cttaaagatt ttgctttttc atccgttaag
    15841 taagggtcaa aagctagtat tttcattccg aaactttgcg cacgtttagc aacacctaaa
    15901 ccaattctac cagcaccaat gacacctaat gttttatgat aaagctcagt acctttaaat
    15961 gcatttcgat tccattcttt atttgtaagt gattggtgtg cttgcggaat atttcgtgcc
    16021 attgataata acattgccag tgtatgttca gtagctgaaa tcgtattacc atctggggca
    16081 ttaataacta aaataccttt taatgttgca gcattaatat taatattatc tacaccaaca
    16141 ccggcgcgtg cgattacttt taaagaatca gcagcattta tgatattttc agtaaccgta
    16201 gtttgactac gaacgattaa agcatggtat gaaggtataa ttttgattaa tgcttcttcg
    16261 gacaagccag tttgaatatc tacattgaat tgttcgtgat ctaataatgc tttgatacca
    16321 tcttttgata tgggatctgc aacgagtaca ttaaattgct tcatgaataa cctccatata
    16381 ttttgatata cctttaccga tatagttaac tttacggtgt tcagttaaaa taatttctaa
    16441 agcagatact accgataaaa tatcgaaagg actaattttc cccatatgac caattcttaa
    16501 aatttgacct ttaagatggc cttgaccacc agcaattgtt attttaaacc gatttttaag
    16561 ttcatctttg attattttca cttcatcatt tgtattaggt ttgaatgctg taaccgttgg
    16621 agatgcatct ttatcattga ctaataaagt taaatctaat gcttttaagg cgcttcttaa
    16681 tgcatttcta attgcatagt gtcgtgctat tacgtgattg aaaccttctg cttttacggt
    16741 ttcaacgtat gcatttacac ctctaaataa agacacattt ggtgtgaacg gtgtagaatt
    16801 gtcagcttgt gacgaaatgt atttatttaa gtctagataa aattttggcg tagttacttc
    16861 tttgaaacgt tcttttgcac ggtggctata agctacaaaa gctaatcctg gaggtaacat
    16921 aattgctttt tgactaccag aaacaagtac atcaatttta tctttgttaa tgtcaacatc
    16981 aacagcacca atgcaactta cgccgtcaac tacaaaataa atattactat taaattgatt
    17041 gatggcattt cctaactcgt gaatagggtg tagcactgtc gtagatgttt cgcaatattg
    17101 actaaatact gctttcactt caacatttaa agttgaaagg aaattgatga aatctttgac
    17161 atctacagct tctccccatg ttacgtcata aatatgcaca tttttgtaat aagtttgtgc
    17221 aatttgttta aatcggttac caaaggcacc tgaaacaatg acaacgaagt gatcttcagg
    17281 gtttacaatg ttcaacatac tagcctccaa gacgcttgta ccgctagatg ttaaaataag
    17341 tacatcattt tgactcccaa atattggctt tagaccttga aatgcttgtt gtgcgatgtc
    17401 ttcaaaatct ttagaacgat gaccaaccat aggtgcttga atttctctca taatggcatc
    17461 aggtacaggg gttgggccag gtgttaataa caacggttga tgataataca tttatatgcc
    17521 tcctataaat aagatttcac tattttagca aattttctga aaatttaaaa gcctaaaaaa
    17581 gtgataacgc tatattgtcg aacaaacgtt atcacttaaa gattttattt ggcctgaatg
    17641 atgggataaa tttttatgtc cacattattt cttacagcat ttgaaatcat gcaattatta
    17701 tctgcaattg ttatcaattt tggtaatcgc ttttctaatt gtgctatttg atcacttgga
    17761 atttgaattt gaggatggtg cacaattttt gacatactga attttccgtt atttaaacaa
    17821 gctgttccaa tcgattgttg ttcaattgaa atatctgtga actttgcacg ttcaagagta
    17881 gctgctaatg agatgatata acatgatgaa gcggctgata ctaacaattc atcgggattt
    17941 gttcctaaac caacaccacc taaggaagca ggtatagaaa tattctctga aagtatgtcg
    18001 ccttgaacgt ttccgacatt gttacgacca ccttgccaag aagtttggac tttaaagtca
    18061 tgttgatgca attgctttaa cctccaatat aattgtgata gtttaatttt agaatagttt
    18121 atcaaagttt aacagaaagg tgacttatca atgactctga ataaactgaa agatgaatta
    18181 caaattgttt cgcaccgtgg attgccgagt gattttcctg aaaatacaat ggtcggttat
    18241 cgagaggtaa tggggctcaa tgttgctatg ttagaaatag atgttcattt gaccaaagac
    18301 caacattttg ttgtgataca tgatgaaaca attgatagaa catcggatgg taaggggcgt
    18361 atttctgatt acacattatc gcaattaaaa tcatttgatt ttggtagtta taaagatgtt
    18421 gcttttaagg gagaacgaat acccacgtta gatgaagtac tttcattatg cttaaagtat
    18481 gataaaaagc tattgattga attgaaaagt ccgaatttat atccagaaat agaatgtaaa
    18541 ttgctggcat ttttggaaga aaagaaagtc gatgcgacac aagtagttat acaatctttt
    18601 gatatcgaat gcatcgaaaa gttaaacaca ttgggcagta tatatgaatt aggtgtgctt
    18661 tgtagtaaac gtaattattg gtataaaaag ccaaactttt caagaattgc tcaaattgct
    18721 agttatgtta atcctaatta tgcattagtg accagaaaat ttgttgataa agcacatcat
    18781 catcaattac aggttatgcc atacacagtt aataaattga aaactggaga aaaattaaga
    18841 caaatgggtg tagatggatt aattacagat aatccgaaat tatttattaa gtgagacgat
    18901 gaatggtaat ttgatagagg gatgaggatt cgtatatcgc aattgataat tttaaaaact
    18961 aatgatgaaa tactaaagat aattaaacaa ataagacttg taaattctaa gttgattgta
    19021 aaatagggta gctccatata attttgaatc tatcattttt aaattaataa aaaaaacacc
    19081 cacaattgtg ggtgtttgta ttgaacgtat tatcttgagt agtactcaac gattaattgt
    19141 tcgttaattt cagcaggtaa ttcgctacgt tctggtaaac gtacgaaagt accagttaag
    19201 ctgtcagcat caaagtttaa gtactcaggt acgaaattgt tgatttcaac tgattcaacg
    19261 atgatgttta atttttgaga tttttcacga actgaaattg tttgaccagg tttaacagaa
    19321 taagatggaa tatcaacacg tttaccatct actaagatat gaccgtggtt aactaattga
    19381 cgtgcttgac gacgagtacg agctaaacct aatgaataaa caacagcgtc taaacgactt
    19441 gctaataaga tcatgaagtt ttcaccgtgt acaccgaatt ttttaccagc gatgtcaaat
    19501 gtgttacgga attgtctttc agtcattcca tataagtaac gtaatttttg tttttcacgt
    19561 aattgtaaac catattctga taatttttta cgttggtttg gaccatgttg tcctggtgcg
    19621 taaggacgtt tttctaattc tttaccagta ccgcttaaag agatacctaa acgacgagat
    19681 tttttccagt ttgaacctct gaatcgagcc ataataagac tcctccttta attctttttt
    19741 gttgttatga ataaacaaaa aagagtgttg catgcttact aagatatatt gttttatgtg
    19801 tcttcacctt atagcgacag ttacgtgaca cgtccgcatt gggaacaaca tagcgctcaa
    19861 taatatacaa ctgctacttt cgttaattca cacaaagatt attgtaacat tttcctgcta
    19921 actgtcaaca ctttattgaa aatgctgatg accttttatg cgagttgctt ttcaataatt
    19981 ttaacaattg cttcaagatg ttctttgtca ttgtcatcaa atcgatcagt tataggggca
    20041 tcgatatcta agacgccgat aattttatca tctttgaaaa ttggaacgac aatctcagac
    20101 ttactattag catcacaagc gatatgtcct tcgaattgat ggacatcagc tacaacttgt
    20161 gtacgacgtt ctgaaacggc tgtaccacat acaccttttc caattggaat gtggacacaa
    20221 gcggggtgtc cttggaaagg tccaagtata agttcatttt gttccaataa gtaaaagccg
    20281 acccaattaa tttgatctag attatcattt aataaggcag acatgttact taaaatagca
    20341 atcatatgat gttcatcttc aataaggctt gctgcttgtt tctttaataa tgtgtagttt
    20401 gttgggttaa ttgttgtcat atttaaaacc tcttattttc atagatttta taatagtata
    20461 gaatttcgaa aatattatgg caataaattc attttataat ttatccttaa gcaaaattac
    20521 gttataatag taatgataaa ttaggaggag aagcatatgg tgttatatat cattttggca
    20581 ataattgtga ttatattgat tgctgtaggt gtattattct atttacgttc aaataaacga
    20641 caaattattg aaaaagcaat cgaacgtaaa aatgaaattg agacgttacc ttttgatcaa
    20701 aaccttgcac aattatctaa gttgaattta aaaggtgaaa caaaaacgaa atacgatgca
    20761 atgaaaaagg acaacgtaga aagtacaaat aagtatctag ctcctgtgga agaaaaaatc
    20821 cataatgctg aggctttatt agataaattt agtttcaacg catctcaaag tgaaattgat
    20881 gatgcaaatg agttgatgga tagttacgaa caaagctatc agcaacaatt agaagatgta
    20941 aatgaaatta ttgcgttata caaagataat gatgaattat atgacaaatg taaggttgat
    21001 tatcgtgaaa tgaaacgtga tgttttagca aatcgtcatc aatttggtga ggcagcaagt
    21061 ctacttgaaa ctgaaattga aaagtttgag ccaaggttag agcaatatga agtactaaaa
    21121 gctgatggta attatgtaca agcgcacaac catatagctg ccttgaatga acaaatgaaa
    21181 cagctaagat cttatatgga agaaatacca gaattaatta gagaaactca aaaagaatta
    21241 cctggtcaat tccaagattt aaaatatggt tgccgtgatc ttaaagttga agggtatgat
    21301 ctggatcacg taaaagtaga cagtacatta caaagcttaa aaacagagct tagtttcgtt
    21361 gaaccattaa ttagccgctt agaattagaa gaagctaatg ataaactagc taatatcaat
    21421 gataagttag atgacatgta tgatttaatt gaacatgaag ttaaagctaa aaatgatgtc
    21481 gaagaaacaa aagatatcat tacggataac ttattcaaag ctaaagacat gaattataca
    21541 ttgcaaacag aaattgaata tgtacgtgaa aactactata taaatgaatc tgatgctcaa
    21601 agtgttcgtc aatttgaaaa tgaaattcaa agtttaattt ctgtatatga tgatatttta
    21661 aaagaaatgt ctaaatctgc tgtgcgatat agcgaggttc aggataattt acaatattta
    21721 gaagatcatg tcacagttat taatgacaaa caagaaaagc tacaaaatca tctgattcaa
    21781 ttgcgtgaag atgaagcaga agcagaagac aatctgctac gagtccaatc gaagaaagaa
    21841 gaagtgtatc gtcgattact tgcttctaac ttaacaagcg ttcctgaaag gtttatcatc
    21901 atgaaaaatg aaattgatca tgaagttcgt gatgttaacg aacaatttag tgaacgtcca
    21961 atacacgtta aacagttaaa agataaagtg tctaaaattg tgattcaaat gaatacattt
    22021 gaagatgaag caaatgatgt tcttgttaat gctgtttatg cagagaaatt aattcaatat
    22081 ggaaatagat atcgtaagga ctatagcaat gttgataaga gcttaaatga agctgaacga
    22141 ttatttaaaa ataatcgcta taagcgtgcg attgaaattg cagagcaagc tcttgaaagt
    22201 gttgagccag gtgttactaa acatattgaa gaagaagtta ttaagcaata gaaactagta
    22261 tgtagttata cttaaataat atgagcactc tgtcaaattg gactgatgag tttaataatt
    22321 gaagttagcc aacgatacgt tgtctagctt cttttttata tggataaatg aaagggacaa
    22381 taaatataaa tagcaattgt ttaaagataa acgtaatcaa atgtgttgtt ttaattaata
    22441 taagtagtga aaaaagcata atcacacagc tgtttaaata gagtgaaata gtctaatttt
    22501 tatttaataa gtagaaataa gattattttg cgctataacc tttatttttt gtcggataat
    22561 agtataatgc ttgagtaaaa caattttaac gattgatgat taatgaaaga gaagtgttta
    22621 ttttgatata tctagataat gcggcaacga cgaaagcatt tgaagaagtg ttagatactt
    22681 atttaaaagt aaatcaatca atgtattata atccgaatag tccgcataaa gctggtttgc
    22741 aggcaaatca attactacaa caagcaaaag cccaaattaa tgcaatgatt aattcaaaaa
    22801 caaattatga tgttgtattc actagtggtg caactgaatc caataatctt gctttaaaag
    22861 gtattgccta tcgtaaattt gatacagcga aggaaataat tacatccgtg ttagagcatc
    22921 cgtccgtatt agaggttgta agatatttgg aagcacacga aggatttaaa gttaaatatg
    22981 ttgatgtaaa gaaagatggc agtattaact tagaacactt caaagaatta atgtcagaca
    23041 aagtcggttt agtaacatgt atgtatgtaa ataatgtaac tggacaaata cagcctattc
    23101 cacaaatggc taaagttata aaaaattatc ctaaggcaca ttttcatgta gatgcggttc
    23161 aagcattcgg caaaatttca atggatctca ataacataga tagtattagt ttaagtggac
    23221 acaagtttaa tggtttaaaa ggccaaggcg tcttacttgt aaatcacatt caaaatgttg
    23281 aaccaactgt ccatggtggt ggtcaagaat atggcgttag aagtggaaca gttaatttgc
    23341 caaatgatat tgcaatggtt aaagcgatga agatagctaa tgaaaacttt gaagcattga
    23401 atgcatttgt tactgtgtta aataatgacg tccgtcaatt tttaaataaa tatcatggag
    23461 tttatattaa ttcttcaact tctggttcac cattcgtttt aaatattagt tttcctggcg
    23521 taaaaggtga agtattagtt aatgcttttt caaaatatga cattatgata tctacgacga
    23581 gtgcttgttc atctaaacgt aataaattaa atgaagtatt ggctgcaatg ggattatcag
    23641 acaaatctat tgaaggtagt ataagattat catttggggc tactacaact aaagaagata
    23701 tagcaaggtt taaagaaata tttatcatca tttatgagga aattaaggag ttgctaaaat
    23761 aatgaagtat gatcacttgc ttgttagata cggggagtta acattaaagg gttcaaatag
    23821 aaaaaaattt gtaaatcaat taagaaataa tgtaaataag tcattaaaag gacttgatgg
    23881 gtttgtcgtt aaaggcaaac gagatcgtat gtatattgaa cttgaagacc atgcagatat
    23941 aaatgaaata acatatcgat tatcaaaaat tttcggtatt aaatctatta gtccagtatt
    24001 aaaagtagaa aaaacaatag aggcaatgag tgcagcggca attaaatttg cgcagcaatt
    24061 tgaagaaaac agcacattta aaattgatgt gaagcgtgcc gataaaaatt tcccaatgga
    24121 tacgtatgaa ttacagcgtg aattgggtgg tgcaatattg aagcacttcg acaatatttc
    24181 agtgaatgtc aaacgtccag atcatgaaat tcgagtggaa gttagattag atgcaattta
    24241 tatgtatgaa gaagttgttc cgggttcagg tggattacca gttggtactg gtggtaagac
    24301 gttactaatg ctttcaggcg gtatagactc accagttgct gggatggaag tgatgagacg
    24361 tggcgtaaca attgaagcga ttcatttcca tagtccacca tttacaagtg atcaagcaaa
    24421 agaaaaagtt attgaattga cacgtatttt agctgaacgt gttggaccaa ttaaattgca
    24481 tattgtacca tttacagaat tgcaaaaaca ggtaaataaa gttgtacatc caagatatac
    24541 aatgacttca acgagacgta tgatgatgcg tgttgctgat aaattagtac atcaaatagg
    24601 ggctttagct attgtaaatg gtgaaaacct agggcaggta gccagtcaaa cacttcatag
    24661 catgtatgca attaataatg taacttctac tcctgtatta cgtcctttat taacttacga
    24721 taaagaagaa attattatta aatcgaaaga aattggtaca tttgaaacat ctattcaacc
    24781 atttgaagat tgttgtacaa ttttcacccc taaaaatcca gtaaccgaac caaactttga
    24841 taaggtagtt caatatgaaa gcgtctttga ttttgaagag atgattaatc gtgctgttga
    24901 aaatattgaa acacttgaaa taactagtga ttataaaact attaaagaac agcaaacaaa
    24961 ccaattaata aatgactttt tataaataaa atcctagagt aaatttaaac ataaggggat
    25021 gttaaactat ggatttgaac ttaacgatga ttataatcat aattttattt ggttttatcg
    25081 cggcgtttat agattcggtt gtagggggtg gcggtttaat ttctacgcca gcattattag
    25141 caatcggtct accaccatct gtggctttag gtacaaataa attggcaagt tcatttggtt
    25201 ctttaactag tacgataaag tttataaggt ccggtaaagt ggacttatat gttgttgcca
    25261 aattatttgg ttttgtattt ttggcatctg catgtggcgc atatattgca acgatggttc
    25321 cgtcacaaat attgaaacct ttaatcatta ttgcactttc gtcggtgttt atattcacat
    25381 tacttaaaaa agattggggc aatacacgta cgtttactca atttacattt aagaaagcca
    25441 tactatttgc agcacttttt atattaatcg gcttttatga tggatttgta ggtgggggaa
    25501 caggttcatt tatgcttttt gtattgttag tctttggttt tgatttttta agtgcagcag
    25561 gaaatgctaa agttttgaac tttgcttcta atataggtgc gcttgtatta tttatggtat
    25621 taggacaagt agattatgta ataggtttaa taatggctac aagcatgatt gctgggtcat
    25681 atactggggc acattttgct atcaaacaag gtgttggcta tgtaaaagta ttatttatta
    25741 ttattactgc tattttaatt ttaaaaaatg cttttgatta tattcaacaa tttgtataaa
    25801 attaaatttg cttttgatta aagcatgaaa attgtaatca aaccataaat tgtcgtatga
    25861 tgtagttaga attttaaaat gcaggaggtc aagtatatga ctgaaataac attcaaaggt
    25921 ggaccaatcc acttaaaagg tcaacaaatt aatgaaggtg attttgcacc tgattttaca
    25981 gtgttagata atgacttaaa tcaagtaaca ttagcagatt atgctggtaa aaagaaatta
    26041 attagtgtgg taccatcaat tgatacaggt gtttgtgatc agcagactcg caaattcaac
    26101 tctgatgctt ctaaagaaga ggggattgtg cttacaattt cagcagactt accattcgca
    26161 caaaaaagat ggtgcgcttc agcaggttta gacaatgtca ttacattaag tgaccaccgt
    26221 gacttatcat ttggtgaaaa ctatggcgtt gttatggaag aacttcgctt attagctcgt
    26281 gcagtatttg tattagatgc agataataaa gttgtttata aagaaatcgt tagtgaaggt
    26341 actgatttcc cagattttga tgctgcttta gctgcataca aaaatattta atcattaaag
    26401 agataaatct taaaatgtat acatcgtgtc catcgttgtc aacagcatta aaatagaatt
    26461 gttttctatg attgctaaga cctatgggca ctttttattg gagagggacg aatatggcag
    26521 aacaacaaac aattatggaa cgcttgtttc atacattaga tgaaaaagct aaaacattaa
    26581 ataatgaaaa tggccaaagt tttattgaaa atcttgggct agcaatggaa caagtatata
    26641 ccaatgaaag aggattgtta gaacaatcga cgttacaaga tcgacgtaaa gcattccaat
    26701 ttgcatattt aagtttaatg caggaagaaa agatacaagc aaatcatcaa attacaccag
    26761 attcaattgg attgatacta ggatttttag ttgagcgttt tatgaacaac caagaagaat
    26821 tacatattgt tgatattgca agtggtgccg gtcatttaag tgctactgta aaagaagtgt
    26881 tacctgaaat tgcggttatg catcatttaa ttgaagttga tccagtttta tcacgtgtta
    26941 gtgtacattt agcaaacttc ttagaaattc ctttcgatgt gtatcctcaa gatgccatca
    27001 tgccactacc attagaagaa gcagatatcg ttattggtga ttttccagta ggctattatc
    27061 caattgatga aagaagtaag gagtttaagc taggttttga agaaggacat agttattcac
    27121 attatttatt aatagaacaa gcaataaatg cattaaaaga tgctggatat gcctttctag
    27181 tggtaccaag taatattttt acaggtgaac atgtaaaaca gcttgaaaaa tatattgcaa
    27241 cagagacaga gatgcaagca tttttaaatt taccaccaac tttatttaaa aatgaaaaag
    27301 cgcgaaaatc tatattaatt ttacaaaaga aaaaatcggg tgaaacaaag ccagttgaag
    27361 tattattggc aaatattcct gatttcaaaa ttccttcaca atttcaagga tttatgacag
    27421 agttaaatca gtggatggac acaaatcgtc ctaaaaaata atctgtaata gtatagtcat
    27481 aaaactgtat gaatgataaa atgaaaaatg aaatagtata aaaagcaaac gatggaggat
    27541 cattacttat gtcaaaatta atcttggcta tcaatgctgg tagttcatca ttaaaatttc
    27601 aattaattag aatgcctgaa gaggaattag taacaaaagg tttagtagaa agaatcggat
    27661 tgaaggattc aatttttaca attgaagtca acggggaaaa agttaaaaca gtacaagata
    27721 tcaaggatca cgttgaggca gttgatatca tgttggatgc ttttaaagcg cacaatatta
    27781 ttaatgatat taacgatatc gatggaacag gtcaccgtgt tgtacatggt ggtgaaaaat
    27841 tccctgaatc agtagctatt actgatgagg ttgaaaagga aattgaagaa ttaagcgaat
    27901 tagcaccgct tcacaaccca gctaacttga tgggtattcg tgctttccgt aaattattac
    27961 caaatatccc tcatgtggca atttttgata cagcattcca tcaaacaatg cctgaaaaag
    28021 catatctata tagcttgcca tatcattatt ataaagatta tggcattcgt aagtatggtt
    28081 tccatggtac aagccataaa tttgtatcac aaagagcggc agaaatgtta gataagccaa
    28141 ttgaagattt acgtattatt tcatgtcaca ttggtaatgg agcatcaatt gcagctatcg
    28201 atggcggtaa atctattgat acatcaatgg gcttcacacc acttgctggt gtaacaatgg
    28261 gtactcgttc tggtaatatt gaccctgctt taattccatt tattatggaa aaaacaggta
    28321 aaactgctga acaagtattg gaaatcttaa acaaagaatc aggtttatta ggattatcag
    28381 gtacatctag tgatttacgt gacttatcag aagaagctga atcaggtaaa gcacgttcac
    28441 aaatggcttt agatgtattt gcttctaaaa ttcacaaata tattggatca tatgctgcga
    28501 gaatgcatgg tgttgatgtt atcgtattta cagcaggtat cggtgaaaac tcagtagaaa
    28561 ttcgtgcgaa agttcttgaa ggcttagaat ttatgggtgt ttactgggat cctaagaaaa
    28621 atgaaaactt actacgtggt aaagaaggat ttattaacta tcctcattca ccagttaaag
    28681 ttgtcgttat tccaacagat gaagaaagta tgattgcccg tgatgttatg acattcggtg
    28741 gtttaaaata atttttaaat aaatattaag cgtctatctc cgctacaatt atatgtagga
    28801 ggtagacgct ttttgtattg tttgaaaatt aactagtgta atgtctcttt tcacgaatat
    28861 cttaaagaat taagtagtcg aagaagatta tgaaaacatt attaagtgag ttttatttat
    28921 aaacaataaa aaaccaagtg cagaaagttg cacttggttc ataatgatca agtagttatc
    28981 taatgatcat ctatatttaa ttttgatatt tttcacgtaa ttgagttgtt gcaacttgtg
    29041 gttggaagtc tgctggtaac tcttcagtac gaacaactaa cacgtcacat ggcgcatgac
    29101 gaacgataga ttctgataca gaaccaacaa taaatctttc cacggcattt aagcctgatg
    29161 taccactcat aatcaagtct gcattaattt catgtgcaag ctttttagga atgatagatt
    29221 taggagagcc aaactctaga cgcgtttcta catctttaac accagcgtta gtagctactt
    29281 ctttataacc atttaataat tcttctgcaa aatgcttaga tttttcagta aattgagcat
    29341 cataaacttc ataagaagaa tacgttcttg aatcaattac attcacaatt gttaacttcg
    29401 catcgttacg tttagcaaca ccaactgctc tgttaaatgc ccattccgct tcatgtgaac
    29461 cgtcaactgc gattaaaata tttttgtaag taatcatagt aagtgcctcc ttgttctttc
    29521 ttaattatat tttaccacaa ttttcggaaa agtcagtgta ataaccttta caattttgtg
    29581 gatttttgat tgcgctttca aaatgttcgc gttaagatga ttatggaggt ggatggtatg
    29641 aaaattggta taccaaggga gattaaaaat aatgaaaatc gtgttggttt atcaccaagt
    29701 ggtgtgcacg ctttagtaga aagtgggcat actgttttag tggaaacaaa tgcgggttca
    29761 ggatcattct ttgaagatgt agattacaaa gaagcaggtg ctgagattgt tgctgaacaa
    29821 gcaaaagttt gggatgtgga tatggttatt aaagttaaag aaccacttga atctgaatat
    29881 ccatatttta aagaagggct tgtattattc acttatcttc atttagcaaa tgaagaaaaa
    29941 ttaacacaag ctttgataga tagaaaagta attagtattg catatgagac tgtgcagtta
    30001 ccagaccgat ctttaccatt gttatcacca atgagtgagg tagcaggaag aatgtcagct
    30061 caagttggcg cagagttcct acaaaaactt aatggtggta tgggaattct actaggtggt
    30121 gtcccaggag tacctaaggg taaagtaact attatcggtg gtggtcaagc aggaacaaat
    30181 gcagctaaaa ttgcactagg actaggtgca gatgttacga ttttagatgt taatccaaag
    30241 cgtttacaac aattagatga tttattcggt ggacgtgtac atacaattat gtcaaatccg
    30301 ttgaatattg agttgtatgt taaacaaagt gatttagtaa taggtgcagt tttaattcca
    30361 ggtgctaaag cgccaagact tgtaacagaa gacatgatta aacaaatgaa aaatgggtca
    30421 gttattattg acattgctat tgatcaaggc ggtatttttg aaacaactga taaaattacg
    30481 acacatgatg atcctacata tattaagcat ggtgtggttc attatgcagt tgcaaatatg
    30541 ccaggtgcag taccgcgtac ttcgacgtta gctttaaata atgctacgct accttatgcg
    30601 ctcatgctag ctaataaagg gtatagagaa gcatttaaat caaatcaacc attatcatta
    30661 ggtttaaata cttacaaagg tcacgtaacc aataaaggcg ttgcagaggc atttgaaatg
    30721 gaatataaat ctgtagaaga agcattacaa ttataatttg aatttgatga aaagggaatg
    30781 gattacagcg tactaaagaa tgacgttatc atctccttgg ttattaaaat taaataatga
    30841 taaattaaag ctagaaacaa tgcgaagaaa tcatcgaaca tgaatgatat tgatttgatt
    30901 cattacatgt tactgatgaa catacctgca tatgtgtttc tagcttgttt gttacatatt
    30961 gcatttagac ttggataaaa atttatgtct taggcgctac aatgttatat tggcagtgtt
    31021 tgactgaatg agaatgcgct tgtaacaagc ttttcatttt tatcccactc cttatttttc
    31081 gtaatgtgtt aatacttcat atccttcatt agtgacaagt atgtcatctt caattcttac
    31141 acctgcaaca ccaggtacat aaatacctgg ttcgattgta ataaccatgc cagcttctaa
    31201 caaattagaa ttagtacttg aaacatcttg atattcatgt tcttgtaatc ctaggccatg
    31261 acctaagcga tgagggaaat attcaccata acctttttct gaaataatat ttctagcgat
    31321 atgatcgata tcttttaatg gtattccagg tttaattgct tggattgcag atgtttctgc
    31381 ttctaataca atattataaa tttcttgtgc ttctttgcta ggttcaccaa atttaatagt
    31441 acgtgtcata tcgctacaat aatgctcata aattacacct aaatcaaata gtacatattc
    31501 attgcttttt aatctgcgat ctcctggtgt gccatgaggt gatgcggcat gatctccaaa
    31561 taaaaccatc gtatcaaaac tcatttcatt gacgccatat tgtttgatag tttgctcaat
    31621 atggttgact acttcacgtt cagtcacacc ttcttttaaa taagaaacac ctatttcgat
    31681 acacttatct gctaactcag cagctttacg tattttgcta atttcatctt cggatttaat
    31741 atttctcaat tgtttgattg ttaaatcaac atctccgaat gaattgacat tgaaaccaga
    31801 gattaattgt ttttggcgtg ctactgttaa gtgctcgctt tcaattagta atttattgat
    31861 tgtttgagga taaagtgaaa aagggttttc agtgtctaaa tatccaacga tttcacctgt
    31921 gaaaggtgat gctttgactt cttcgacttc catttttgga caaaatagta cttgtttacc
    31981 atctttctta atcaataatg caaataatct ttcatggggt tcgctacggt atccagtaaa
    32041 ataatataca ttcaacggtg ttgtaatcca tgctgcatca gcttgttgat tgttcaattc
    32101 gtctattatt tttgatattt ttgtcatttt ataaacactc ctcaacataa tattacttcc
    32161 attttatgcc aattattatt agatttcaaa atcgagctaa tacattaact agattttcaa
    32221 tttttaaaat ttgagaattt gttataatta agtggcaaaa gggtactaaa tttatatagt
    32281 aattgatagg aggatgctca aatgaagtta tcatttcatg gtcaatcaac gatttattta
    32341 gaagggaaca acaaaaaggt tatcgtagat ccgtttattt caaataaccc taagtgtgat
    32401 ttgaatattg aaacagtgca ggtagactat atcgttttaa cgcacggtca ttttgatcat
    32461 tttggtgatg ttgtggaact agcaaagaaa acaggagcaa cagtaattgg aagtgcagaa
    32521 atggctgact atctttcttc atatcacggt gttgaaaatg ttcatggtat gaatattggt
    32581 ggtaaagcaa actttgattt cggaagcgtt aaatttgttc aagcatttca tagttcaagt
    32641 tttacacatg aaaatggtat tccagtttat ttaggtatgc caatgggtat cgtgtttgaa
    32701 gttgaaggaa aaacaattta tcatactggt gatacaggtc tgtttagtga catgtcactt
    32761 attgcgaaac gtcatccagt tgatgtatgt tttgtaccaa ttggagataa ttttacgatg
    32821 ggtattgatg atgcaagtta tgctattaat gaatttatta aaccaaaaat ttctgtaccg
    32881 attcattatg atacattccc attaatcgaa caagatccac aacaatttaa agatgctgtt
    32941 aatgtagggg acgtgcaaat tttaaaacca ggtgaatctg ttcagtttta aatagaatat
    33001 agattttaca ctaaaaaaca cctcgctaat ctatgtggaa gttatcgtct gcatattttt
    33061 agtgggtgtt tttatattct actgattcag ttacgacgtg gacgggggac atcagtggtt
    33121 ctttatcatt tctgacctac tctcgtaatt ttagttttat acaattagac attgattata
    33181 aatttactac gatataaaaa atccagaacc acagtcataa tcgtttcttg tatttactca
    33241 gtcgtttgag actgcatcat gattgaaaga ttatttgtga ttctggataa aaattttatt
    33301 taacgattaa tacagggata gtcgcacgtt ttgctacttt gtggctgaca cttccaagta
    33361 caaatttctt tttgtcttct gctttacggt tacttaaaac aacaatttcg tatttaccgc
    33421 tattagcatg ttttaatagt tcttcttttg cattaccacg aacaattatt tggtcataat
    33481 caataccata atcttctaaa gctttgcgtg ttttttccaa tcgcttgctt cgttcttcag
    33541 taagtttgtt taaatgaaca cctgctttaa ttgatgcttg agcatcttgt tcgctgattg
    33601 cgtttaaaac tgttacgact gtaccttcgc cagctaattt agacacttct tttagtgctt
    33661 tttcattttt taactgagtg tctacaccaa gtaatatatt ttta
//
LOCUS       DAMZKC010000008        33358 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.008, whole genome shotgun sequence.
ACCESSION   DAMZKC010000008 DAMZKC010000000
VERSION     DAMZKC010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 33358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33358
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.008"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(62..229)
                     /locus_tag="POB55_000278"
     CDS             complement(62..229)
                     /locus_tag="POB55_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499274.1"
                     /translation="MRYVISIIMGIVLAIWSFKQLSQSHLDSGFIFFFIVYVLCISYF
                     NSDKHDKNKKR"
     gene            complement(341..1885)
                     /gene="pruA"
                     /locus_tag="POB55_000279"
     CDS             complement(341..1885)
                     /gene="pruA"
                     /locus_tag="POB55_000279"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HDI6499275.1"
                     /translation="MVVEFKNEPGYDFSVQENVDMFKKALKDVEKELGQDIPLVINGE
                     KIFKDDKIKSINPADTSQVIANASKATKQDVEDAFKAANEAYKSWKTWSANDRAELML
                     RVSAIIRRRKAEIAAIMVYEAGKPWDEAVGDAAEGIDFIEYYARSMMDLAQGKPVLDR
                     EGEHNKYFYKSIGTGVTIPPWNFPFAIMAGTTLAPVVAGNTVLLKPAEDTPYIAYKLM
                     GILEEAGLPKGVVNFVPGDPKEIGDYLVDHKDTHFVTFTGSRATGTRIYERSAVVQEG
                     QNFLKRVIAEMGGKDAIVVDENIDTDMAAEAIVTSAFGFSGQKCSACSRAIVHKDVYD
                     EVLEKSIKLTKELTLGNTVDNTYMGPVINKKQFDKIKNYIEIGKEEGKLEQGGGTDDS
                     KGYFVEPTIISGLKSKDRIMQEEIFGPVVGFVKVNDFDEAIEVANDTDYGLTGAVITN
                     NREHWIKAVNEFDVGNLYLNRGCTSAVVGYHPFGGFKMSGTDAKTGSPDYLLHFLEQK
                     VVSEMF"
     gene            complement(2075..2674)
                     /locus_tag="POB55_000280"
     CDS             complement(2075..2674)
                     /locus_tag="POB55_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDI6499276.1"
                     /translation="MTEKEKMLAEKWYDANFDQYLINERARAKDICFELNHTRPSATN
                     KRKELIDQLFQTTTDNVSISIPFDTDYGWNVKLGKNVYVNTNCYFMDGGQITIGDNVF
                     IGPNCGFYTATHPLNFHHRNEGFEKAGPIHIGSNTWFGGHVAVLPGVTIGEGSVIGAG
                     SVVTKDIPPHSLAVGNPCKVVRKIDNDLPSETLNDETIK"
     gene            2913..3104
                     /gene="cwrA"
                     /locus_tag="POB55_000281"
     CDS             2913..3104
                     /gene="cwrA"
                     /locus_tag="POB55_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall inhibition responsive protein CwrA"
                     /protein_id="HDI6499277.1"
                     /translation="MRILITGTVAILIILGLVKTIQDHEMTNDTSRQLSDNKDDDKVI
                     HLNNFKNLHAKEFNPSDFF"
     gene            3344..5752
                     /locus_tag="POB55_000282"
     CDS             3344..5752
                     /locus_tag="POB55_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HDI6499278.1"
                     /translation="MANTKKTTLDITGMTCAACSNRIEKKLNKLDDVNAQVNLTTEKA
                     TVEYNPDQHDVQEFINTIQHLGYGVAVETVELDITGMTCAACSSRIEKVLNKMDGVQN
                     ATVNLTTEQAKVDYYPEETDADKLVTRIQKLGYDASIKDNNKDQTSRKAEALQHKLIK
                     LIISAVLSLPLLMLMFVHLFNMHIPALFTNPWFQFILATPVQFIIGWQFYVGAYKNLR
                     NGGANMDVLVAVGTSAAYFYSIYEMVRWLNGSTTQPHLYFETSAVLITLILFGKYLEA
                     RAKSQTTNALGELLSLQAKEARILKDGNEVMIPLNEVHVGDTLIVKPGEKIPVDGKII
                     KGMTAIDESMLTGESIPVEKNVDDTVIGSTMNKNGTITMTATKVGGDTALANIIKVVE
                     EAQSSKAPIQRLADIISGYFVPIVVGIALLTFIVWITLVTPGTFEPALVASISVLVIA
                     CPCALGLATPTSIMVGTGRAAENGILFKGGEFVERTHQIDTIVLDKTGTITNGRPVVT
                     DYHGDNQTLQLLATAEKDSEHPLAEAIVNYAKEKQLTLTETTTFKAVPGHGIEATIDH
                     HHILVGNRKLMADNDISLPKHISDDLTYYERDGKTAMLIAVNYSLTGIIAVADTVKDH
                     AKDAIKQLHDMGIEVAMLTGDNKNTAQAIAKQVGIDTVIADILPEEKAAQIAKLQQQG
                     KKVAMVGDGVNDAPALVKADIGIAIGTGTEVAIEAADITILGGDLMLIPKAIYASKAT
                     IRNIRQNLFWAFGYNIAGIPIAALGLLAPWVAGAAMALSSVSVVTNALRLKKMRLEPR
                     RKDA"
     gene            6101..6307
                     /gene="copZ"
                     /locus_tag="POB55_000283"
     CDS             6101..6307
                     /gene="copZ"
                     /locus_tag="POB55_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HDI6499279.1"
                     /translation="MSQEILNVEGMSCGHCKSAVESALNNIDGVTSADVNLENGQVSV
                     QYDDSKVAVSQMKDAIEDQGYDVV"
     gene            complement(6397..7395)
                     /locus_tag="POB55_000284"
     CDS             complement(6397..7395)
                     /locus_tag="POB55_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HDI6499280.1"
                     /translation="MTKIKIMSVRDEDMPYIKAWAEKHHVEVDITKEALTDDNVEGVA
                     GYDGLSLSQQIPLSEHVYKRLNELDIKQIAQRSAGFDTYDLELANKYNLIVSNVPSYS
                     PNSIAEFAVNQAINVVRHFNQIQTKVREHDFRWEPTILSKSIKDLKVAVIGTGRIGRV
                     VADIFANGYQSDVVAYDPFPNAKIATYVDYKDTIEEAVEGADIVTLHVPATKYNHYLF
                     NAELFKHFKKGAVFVNCARGSLVDTKALLDALDNGVIKGAALDTYEFERKLFPSDQRG
                     KTLNDPLLESLIDREDVILTPHIAFYTEAAVKNLIVDALDATLDVLQTGDTRLRVN"
     gene            complement(7418..8572)
                     /locus_tag="POB55_000285"
     CDS             complement(7418..8572)
                     /locus_tag="POB55_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDI6499281.1"
                     /translation="MISNKLANIPDSYFGKTMGRKIEHGPLPLINMAVGIPDGPTPQG
                     IIDHFQQALTIPENQKYGAFHGKEAFKQAIVDFYQRQYNVTLDKEDEVCILYGTKNGL
                     VAVPTCVINPGDYVLLPDPGYTDYLAGVLLADGKPVPLNLEPPHYLPDWSTVDSQIID
                     KTKLIYLTYPNNPTGSTATKEVFDEAIAKFKDTDTKIVHDFAYGAFGFDAKNPSILAS
                     ENGKDVAIEIYSLSKGYNMSGFRVGFAVGNKDMIQALKKYQTHTNAGMFGALQDAAIY
                     ALNHYDDFLEEQSNVFKTRRDRFEAMLAKADLPFVHAKGGIYVWLETPPGYDSEQFEQ
                     FLVQEKSILVAPGKPFGENGNRYVRISLALDDQKLDEAAIRLTELAYLYE"
     gene            complement(8999..10507)
                     /locus_tag="POB55_000286"
     CDS             complement(8999..10507)
                     /locus_tag="POB55_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDI6499282.1"
                     /translation="MKIAVIGAGVTGLAAAARIASQGHEVTIFEKNNNVGGRMNQLKK
                     DGFTFDMGPTIVMMPDVYKDVFTACGKNYEDYIELRQLRYIYDVYFDHDDRITVPTDL
                     AELQQMLESIEPGSTHGFMSFLTDVYKKYEIARRYFLERTYRKPSDFYNMTSLVQGAK
                     LKTLNHADQLIEHYIDNEKIQKLLAFQTLYIGIDPKRGPSLYSIIPMIEMMFGVHFIK
                     GGMYGMAQGLAQLNKDLGVNIELNAEIEQIIIDPKFKRADAIKVNGDIRKFDKILCTA
                     DFPSVAESLMPDFAPIKKYPPHKIADLDYSCSAFLMYIGIDIDVTDQVRLHNVIFSDD
                     FRGNIEEIFEGRLSYDPSIYVYVPAVADKSLAPEGKTGIYVLMPTPELKTGSGIDWSD
                     EALTQQIKEIIYRKLATIEVFEDIKSHIVSETIFTPNDFEQTYHAKFGSAFGLMPTLA
                     QSNYYRPQNVSRDYKDLYFAGASTHPGAGVPIVLTSAKITVDEMIKDIEQGV"
     gene            complement(10519..11382)
                     /locus_tag="POB55_000287"
     CDS             complement(10519..11382)
                     /locus_tag="POB55_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HDI6499283.1"
                     /translation="MTMMDMNFKYCHKIMKKHSKSFSYAFDLLPEDQRKAVWAIYAVC
                     RKIDDSIDVYGDIQFLNQIKEDIQSIEKYPYEHHHFQSDRRIMMALQHVAQHKNIAFQ
                     SFYNLIDTVYKDQHFTMFETDAELFGYCYGVAGTVGEVLTPILSDHETHQTYDVARRL
                     GESLQLINILRDVGEDFDNERIYFSKQRLKQYEVDIAEVYQNGVNNHYIDLWEYYAAI
                     AEKDFQDVMDQIKVFSIEAQPIIELAARIYIEILDEVRQANYTLHERVFVDKRKKAKL
                     FHEINSKYHRI"
     gene            complement(11419..12546)
                     /gene="crtQ"
                     /locus_tag="POB55_000288"
     CDS             complement(11419..12546)
                     /gene="crtQ"
                     /locus_tag="POB55_000288"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,4'-diaponeurosporenoate glycosyltransferase"
                     /protein_id="HDI6499284.1"
                     /translation="MKWLSRILTVIVTMSMACGALIFNRRHQLKAKTLNFNHKALTII
                     IPARNEEKRIGHLLHSIIQQQVPVDVIVMNDGSTDETARVARSYGATVVDVVDDTDGK
                     WYGKSHACYQGVTHACTNRIAFVDADVTFLRKDAVETLINQYQLQGEKGLLSVQPYHI
                     TKRFYEGFSAIFNLMTVVGMNVFSTLDDGRTNQHAFGPVTLTNKEDYYATGGHKSANR
                     HIIEGFALGSAYTSQSLPVTVYEGFPFVAFRMYQEGFQSLQEGWTKHLSTGAGGTKPK
                     IMTAIVLWLFGSIASILGLCLSLKYRQMSVRKMVALYLSYTTQFIYLHRRVGQFSNLL
                     MVCHPLLFMFFTKIFIQSWKQTHRYGVVEWKGRQYSISKEQ"
     gene            complement(12552..14045)
                     /locus_tag="POB55_000289"
     CDS             complement(12552..14045)
                     /locus_tag="POB55_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDI6499285.1"
                     /translation="MTKHIIVIGGGLGGISAAIRMAQSGYSVSLYEQNNHIGGKVNRH
                     ESDGFGFDLGPSILTMPYIFEKLFEYSKKQMSDYVTIKRLPHQWRSFFPDGTTIDLYE
                     GIKETGQHNAILSKQDIEELQNYLNYTRRIDRITEKGYFNYGLDTLSQIIKFHGPLNA
                     LINYDYVHTMQQAIDKRISNPYLRQMLGYFIKYVGSSSYDAPAVLSMLFHMQQEQGLW
                     YVEGGIHHLANALEKLAREEGVTIHTGARVDNIKTYQRRVTGVRLDTGEFVKADYIIS
                     NMEVIPTYKYLIHLDTQRLNKLEREFEPASSGYVMHLGVACQYPQLAHHNFFFTENAY
                     LNYQQVFHEKVLPDDPTIYLVNTNKTDHTQAPVGYENIKVLPHIPYIQDQPFTTEDYA
                     KFRDKILDKLEKMGLTDLRKHIIYEDVWTPEDIEKNYRSNRGAIYGVVADKKKNKGFK
                     FPKESQYFENLYFVGGSVNPGGGIPMVTLSGQQVADKINAREAKNRK"
     gene            complement(14038..14526)
                     /gene="crtO"
                     /locus_tag="POB55_000290"
     CDS             complement(14038..14526)
                     /gene="crtO"
                     /locus_tag="POB55_000290"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl-4,4'-diaponeurosporenoate
                     acyltransferase"
                     /protein_id="HDI6499286.1"
                     /translation="MKKYIKTAFFCSMYWLIVQLNIANLGTRIPDKYFRQKYIIFKSF
                     NFEKHGKFWNKWFYVRKWKHKILDGHQLNQNIYDQRHLMTINTDEIEKMIIETKRAEL
                     IHWISILPVIIFNKGPRLVKYINIFYAMIANVPIIIVQRYNRPRLTQLLRILKRRGER
                     HD"
     gene            complement(14701..15468)
                     /locus_tag="POB55_000291"
     CDS             complement(14701..15468)
                     /locus_tag="POB55_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDI6499287.1"
                     /translation="MKKIVTATIATAGLATIAFAGHDAQAAEQNNNGYNSNDAQSYSY
                     TYTIDAQGNYHYTWTGNWNPSQLTQNNTYYYNNYNTYSYNNASYNNYYNHSYQYNNYT
                     NNSQTATNNYYTGGSGASYSTTSNNVHVTTTAAPSSNGRSISNGYASGSNLYTSGQCT
                     YYVFDRVGGKIGSTWGNASNWANAAASSGYTVNNTPKVGAIMQTTQGYYGHVAYVEGV
                     NSNGSVRVSEMNYGHGAGVVTSRTISANQAGSYNFIH"
     gene            complement(15829..17640)
                     /locus_tag="POB55_000292"
     CDS             complement(15829..17640)
                     /locus_tag="POB55_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDI6499288.1"
                     /translation="MDTKDFKRLEKMYSPRYLPGLDGLRAFAVIGIIIYHLNAQWLSG
                     GFLGVDTFFVISGYLITSLLISEYYRTQKIDLLEFWKRRLKRLIPAVLFLICVVLTFT
                     LIFKPELIIQMKRDAIAAIFYVSNWWYISQNVDYFNQFAIEPLKHLWSLAIEEQFYLL
                     FPLVITFLLHRFKPRNIIQTLFIVSLISLGLMIVIHFITGDNSRVYFGTDTRLQTLLL
                     GCILAFIWPPFALKKDISKKIVVSLDIIGISGFAVLMTLFFIVGDQDQWIYNGGFYII
                     SFATLFIIAIAVHPSSLFAKFLSMKPLLIIGKRSYSLYLWHYPIIVFVNSYYVQGQIP
                     VYVYIIEILLTALMAEISYRFIETPIRKKGFKAFAFLPKKKGQFARTVLVILLLVPSI
                     VVLSGQFDALGKQHEAEKKEKKTEFKTTKKKVVKKDKQEDKQTANSKEDIKKSSPLLI
                     GDSVMVDIGNVFTKKIPNAQIDGKVGRQLVDATPIVKSQYKDYAKKGQKVVVELGTNG
                     AFTKDQLNELLDSFGKADIYLVSIRVPRDYEGRINKLIYEAAEKRSNVHLVDWYKASA
                     GHPEYFAYDGIHLEYAGSKALTDLIVKTMETHATNKK"
     gene            complement(18153..18641)
                     /locus_tag="POB55_000293"
     CDS             complement(18153..18641)
                     /locus_tag="POB55_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6499289.1"
                     /translation="MAINIIEYNRSYKEELIEFILSIQKNEFNIKIDRDDQPDLENIE
                     HNYLNNGGQFWLAINNHQNIVGTIGLIRLDNNMSALKKMFVDKGYRNLKIGKKLLDKV
                     IMTCKEQNIDGIYLGTIDKFISAQYFYSNNGFREIKRGDLPSSFPKLDVDNRFYYRNL
                     KD"
     gene            complement(18917..19618)
                     /gene="isaA"
                     /locus_tag="POB55_000294"
     CDS             complement(18917..19618)
                     /gene="isaA"
                     /locus_tag="POB55_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase IsaA"
                     /protein_id="HDI6499290.1"
                     /translation="MKKTIMASSLAVALGVTGYAAGTGHQAHAAEVNVDQAHLVDLAH
                     NHQDQLNAAPIKDGAYDIHFVKDGFQYNFTSNGTTWSWSYEAANGQTAGFSNVAGADY
                     TTSYNQGSNVQSVSYNAQSSNSNVEAVSAPTYHNYSTSTTSSSVRLSNGNTAGATGSS
                     AAQIMAQRTGVSASTWAAIIARESNGQVNAYNPSGASGLFQTMPGWGPTNTVDQQINA
                     AVKAYKAQGLGAWGF"
     gene            complement(20226..21281)
                     /locus_tag="POB55_000295"
     CDS             complement(20226..21281)
                     /locus_tag="POB55_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HDI6499291.1"
                     /translation="MTSIKTITPKDFIFRVLSGVAIGIVAGLVPNAILGEIFKYFMDY
                     HPIFKTLLGVVVAIQFTVPALIGALVAMKFDLSPLAIAVVASAAYVGSGAAQFKNGAW
                     MITGIGDLINTMITAAIAVLFILLIQHRVGSMALIVFPTVVGGISGAIGVLILPYTKM
                     ITTAIGNMVNGFTELQPIVMSILISMVFSLIIISPLSTVAIAFAIGISGLAAGSASIG
                     ISATEAVLIIGTSKVNRLGVPLSVFFGGVKMMIPNMVKYPILMLPILTTAIVSGLVSA
                     LVGIHGTKESAGFGFIGMVGPINAFKFMEVDSAWLSVLLIVVAFFVVPFVTAWLADII
                     YRKVFRLYTNDIFKFMG"
     gene            complement(21399..21536)
                     /locus_tag="POB55_000296"
     CDS             complement(21399..21536)
                     /locus_tag="POB55_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499292.1"
                     /translation="MDFKNEDKIKVYCNNMFEKLPISLSKNSEFCDILFFIVLSYRCY
                     C"
     gene            21691..22260
                     /locus_tag="POB55_000297"
     CDS             21691..22260
                     /locus_tag="POB55_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDI6499293.1"
                     /translation="MMESKSIDPRIVRTKQLLVDAFLKISREKKLSQITVKDITDIAT
                     LNRATFYAHFTDKEDLLDYTLSVTILKDLNDNLSISNVINEKVLRNIFISIASYIKDA
                     AKSCELNSEAFCNKAHQRINNELEDIFAIMLENSYPEHQRDIIVNSASFLAAGISGLA
                     LHWFNTSQETADVFIDRNLPFLIHHIAHF"
     gene            22458..22958
                     /locus_tag="POB55_000298"
     CDS             22458..22958
                     /locus_tag="POB55_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499294.1"
                     /translation="MTSIFVFAYLNNTSDNMLFRQQMICACLLANVQHSSIVCLNNTC
                     CIVDIRCLNTKIGGIDVSETDEPQGFERTHNILNINQNRQSLMTFIISKLKSTLKQRI
                     INARGKKRIDYRLSYNFYITYYDTVEIKKIDCEYTYAMPIAILIRHTMSFDNAHFFAQ
                     LRHPVL"
     gene            23178..23408
                     /locus_tag="POB55_000299"
     CDS             23178..23408
                     /locus_tag="POB55_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HDI6499295.1"
                     /translation="MKILDRINELANKEKVQPLTVAEKQEQHALRQDYLSMIRGQVLT
                     TFSTIKVVDPIGQDVTPDKVYDLRQQYGYIQN"
     gene            complement(23571..23948)
                     /locus_tag="POB55_000300"
     CDS             complement(23571..23948)
                     /locus_tag="POB55_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDI6499296.1"
                     /translation="MIQSMWFNLHVQDLEKSAQFYKALGFKINRNPQMLDKMVGIQIG
                     QTTVILIENKHFQNVSQQSLNTEPNEVMISLGVNTNEEVDQLVNKVKEAGGTVVQEPT
                     VSQGFYGAMFKDLDGHHFNFLVC"
     gene            complement(23965..24786)
                     /locus_tag="POB55_000301"
     CDS             complement(23965..24786)
                     /locus_tag="POB55_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA/HSCARG family protein"
                     /protein_id="HDI6499297.1"
                     /translation="MKDILVIGATGKQGNAVVKQLLEDGWYVSALTRNKNNRKLSDIG
                     HPHLSIVEGDLSDNVSLQSAMKGKYGLYSIQPIVKDDVSEELRQGMKIIEIAEQENIQ
                     HIVYSTAGGVNRNRTGPHFEVLAKIENRLMESNINATVIKPSFFMDNFLRIAKVEDER
                     ITLPEFINPNIKFTMISSIDIAKIASYIFAHPQSFTHQSIEIGSDEVTLSEAATIFSE
                     VTGKSTVIEGEFVSGVAEKQWLEEKGYEVDFELMAEINPTRLSLSDWLKVQNYNK"
     gene            complement(24806..25105)
                     /locus_tag="POB55_000302"
     CDS             complement(24806..25105)
                     /locus_tag="POB55_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2316 family protein"
                     /protein_id="HDI6499298.1"
                     /translation="MSLNKEQRRITCEELQAHFEESTLSVQMIAEKLNVTTEDVEKVL
                     AMTAPLGIFSHQLQRFIHLVWDVRDVINDNIKGNGQTPEPYTYLKGEKEDYWFLR"
     gene            25237..25794
                     /locus_tag="POB55_000303"
     CDS             25237..25794
                     /locus_tag="POB55_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDI6499299.1"
                     /translation="MRKDAKENRQRIEEIAHKLFDEEGVENISMNRIAKELGIGMGTL
                     YRHFKDKSDLCYYVIQRDLDIFITHFKQIKDDYHSNYEVMQVSLDYLLQFKIDNKALL
                     QCIEAGNNKLRFYQSAFYQELFDFYYDLFKSDDDTYTKFKTDMLLQSLSTSVFAFQIE
                     HRHISIEAYRNYLLNIYLDEVERND"
     gene            25787..26491
                     /locus_tag="POB55_000304"
     CDS             25787..26491
                     /locus_tag="POB55_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HDI6499300.1"
                     /translation="MTKIVLITGGNKGLGYASAEALKALGYKVYIGSRNDVRGQQASQ
                     KLGVHYVQLDVTSDYSVKNAYNMIAEKEGRLDILINNAGISGQFSAPSKLTPRDVEEV
                     YQTNVFGIVRMMNTFVPLLEKSEQPVVVNVSSGLGSFGMVTNPETAESKVNSLAYCSS
                     KSAVTMLTLQYAKGLPNMQINAADPGATNTDLVGDFSNNSKHVSEGIKPIIQLATIGA
                     DGPTGTFINGDGEMPW"
     gene            26589..27599
                     /locus_tag="POB55_000305"
     CDS             26589..27599
                     /locus_tag="POB55_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDI6499301.1"
                     /translation="MKSITFEEHYVIEDIQKETMNAISADPKGVPMKVMLEGLEKKTG
                     FTNADELSHHDERIQFMNNQDVQIQVLSYGNGSPSNLVGQKAIELCQKANDQLANYIA
                     QYPNRFVGFATLPINEPEAAAREFERCINDLGFKGALIMGRAQDGFLDQDKYDIIFKT
                     AENLDVPIYLHPAPVNSDIYQSYYKGNYPEVTAATFACFGYGWHIDVGIHAIHLVLSG
                     IFDRYPKLNMIIGHWGEFIPFFLERMDEALFAEHLNHPVSYYFKNNFYITPSGMLTKP
                     QFDLVKKEVGIDRILYAADYPYIEPEKLGVFLDELGLTDEEKEKISYTNGAKLLGLSA
                     NN"
     gene            27619..28437
                     /locus_tag="POB55_000306"
     CDS             27619..28437
                     /locus_tag="POB55_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDI6499302.1"
                     /translation="METLELQGAKLRYHQVGQGPVLIFIPGANGTGDIFLPLAEQLKD
                     HFTVVAVDRRDYGESELTEPLPDSASNPDSDYRVKRDAQDIAELAKSLSDEPVYILGS
                     IVAMHVLKDYPEVVKKIAFHEPPINTFLPDSTYWKDKNDDIVHQILTEGLEKGMKTFG
                     ETLNIAPIDAKMMSQPADTEEGRIEQYKRTMFWSEFEIRQYTHSDITLDDFTKYSDKI
                     TLLNGTDSRGSFPQDVNFYINKETSIPIVDIPGGHLGYIQKPEGFADVLLNMWG"
     gene            complement(28584..29474)
                     /locus_tag="POB55_000307"
     CDS             complement(28584..29474)
                     /locus_tag="POB55_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDI6499303.1"
                     /translation="MKIVIIGGFLGGGKTTVLNHLLAESLKESLKPAVIMNEFGKMSV
                     DGALVSEDIPLSELTEGCICCAMKADVSEQLHQLYLKEQPDIVFIECSGIAEPVSVLD
                     ACLTPILAPFTTITHMIGVIDASMYKHIKSFPKDIQGLFYEQLAYCSVLFVNKIDSAD
                     VETTSKLLKDLEVINPEADIQVGMHGSVTLPISVRQMTATSDNKHKSLHQMINHQFVQ
                     SPAKCTKAEFIKRLACLPSHIYRLKGFMTFEDTAHTYLIQFTQGQYELTPVAFSKKVP
                     EYLVLIGKGISKEDYQCLEQ"
     gene            complement(29571..30911)
                     /locus_tag="POB55_000308"
     CDS             complement(29571..30911)
                     /locus_tag="POB55_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HDI6499304.1"
                     /translation="MLEVKYDMRTGHYTPIPNEPHYLVISHADKLTATEKAKLRLLII
                     KQKLDISLAESVVSSPIASEHVIEQLTLFQHERRHLRPKISATFLAWLLIFLMFALPI
                     GIAYQFSDWFQNQYVSAWIEYLTQTTLLNHDILQHILFGDYGVLSLGTYSLVWALPVV
                     ILISLSTAIIDQTGLKSWMIWAIEPSMLWIGLQGNDIVPLLEGFGCNAAAISQAAHQC
                     HTCTKTQCMSLISFGSSCSYQIGATLSIFSVAGKSWLFMPYLILVLLGGILHNRIWLK
                     KNDQQLSVPLPYDRQLHMPNIRQMLLQMWQNIQMFIVQALPIFITICLIVSILSLTPI
                     LNVLSQIFTPILSLLGISSELSPGILFSMIRKDGMLLFNLHQGALLQGMTATQLLLLV
                     FFSSTFTVCSVTMTMLLKHLGGQSALKLIGKQMVTSLSLVIGVGIIVKIVMLII"
     gene            complement(30905..32011)
                     /locus_tag="POB55_000309"
     CDS             complement(30905..32011)
                     /locus_tag="POB55_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HDI6499305.1"
                     /translation="MQHHKVAIIGAGAAGIGMAITLKDFGITDVIILEKGTVGHSFKH
                     WPKSTRTITPSFTSNGFGMPDMNAISMDTSPAFTFNEEHISGETYAEYLQVVANHYEL
                     NIFENTVVTNISVDDAYYTIATTTEIYHADYIFVATGDYNFPKKPFKYGIHYSEIEDF
                     DNFNKGQYVVIGGNESGFDAAYQLAKNGSDIALYTSTTGLNDPDADPSVRLSPYTRQR
                     LGNVIKQGARIEMNVHYTVKDIDFNNGQYHISFDSGQSVHTPHEPILATGFDATKNPI
                     VQQLFVTTNQDIKLTTHDESTRYPNIFMIGATVENDNAKLCYIYKFRARFAVLAHLLT
                     QREGLPAKQDVIENYQKNQMYLDDYSCCEVSCTC"
     gene            complement(32306..32602)
                     /locus_tag="POB55_000310"
     CDS             complement(32306..32602)
                     /locus_tag="POB55_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4176 domain-containing protein"
                     /protein_id="HDI6499306.1"
                     /translation="METIGSIIYLKEGSQKLMIINRGPIVEIENQKYMFDYSACKYPI
                     GVVEDEIYYFNEENIDSVIFKGYSDQDEVRFQELFENMKQNLDSEIQRGEVTPQ"
     gene            complement(32590..32958)
                     /locus_tag="POB55_000311"
     CDS             complement(32590..32958)
                     /locus_tag="POB55_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499307.1"
                     /translation="MSNKLDEINKIITAKHKQMDDLYDEKREVKALIDESDALNHSID
                     QLYQHLGERYYSSNMASRMEQFRDEFHFAKRRSTEALYEQQQQIQHGIRKAEEEMIDL
                     EMRRNVEIETVTKEENKWKQ"
     gene            complement(32973..33251)
                     /locus_tag="POB55_000312"
     CDS             complement(32973..33251)
                     /locus_tag="POB55_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04197 family type VII secretion effector"
                     /protein_id="HDI6499308.1"
                     /translation="MMSTVQSDIFKTNSASSSIKSAVETCNNVSKPDKDESTTVSGNN
                     NAHSVIDDLMSKNQSVAEAIRTASDNIQKVGEAFDQTDVMIGNEIGKN"
BASE COUNT        10717 a         6250 c         5119 g        11272 t
ORIGIN      
        1 atgagcgtta ccaacattca taataaaaac gatagtgtac acgttaatga attcgtgtac
       61 actatcgttt tttattttta tcatgcttat cgctattaaa ataactgata cacaacacat
      121 aaactatgaa gaaaaaaata aatccgctat ctaaatgact ttgactcagt tgtttaaatg
      181 accaaattgc taatacaatt cccattatta ttgaaataac gtatctcaca ttcttatacc
      241 tataatcctt ttctaaaaat atggttgcta ttacttaatt tttaaagtta taaataaaaa
      301 gagccaaccg caatggatgg cccttgttca ttatgaagca ttagaacatt tctgaaacaa
      361 ccttttgttc taagaagtgt aataagtagt ctggactacc tgttttagcg tccgtacctg
      421 acattttgaa accaccaaat ggatggtatc caacaactgc tgaagtacag cctctgttaa
      481 ggtataaatt gcctacatca aattcgttta ccgctttaat ccaatgctcg cgattatttg
      541 taatcactgc accagttaaa ccgtaatctg tatcatttgc aacctcaatt gcttcatcaa
      601 aatcgttaac tttcacaaag ccaacaactg gaccaaaaat ttcttcttgc atgattctat
      661 ctttagattt aagtcctgaa atgattgttg gttctacaaa gtaacctttt gaatcatcag
      721 tgccgccacc ttgttctaat ttaccttctt ctttaccaat ctcaatataa tttttaatct
      781 tatcaaattg ttttttatta ataactgggc ccatatacgt attgtctaca gtattgccca
      841 acgttaattc ttttgttaat ttgattgatt tctctaatac ttcgtcataa acgtctttat
      901 gcacaattgc acgtgaacat gctgaacatt tttgaccaga aaaaccaaat gctgacgtta
      961 caatagcttc tgctgccata tctgtatcaa tattttcatc aactacaatg gcatctttac
     1021 cacccatttc agcgataaca cgtttcaaga agttttgacc ttcttgaaca acggcactac
     1081 gttcataaat tctagtacct gtcgcacgtg atcctgtaaa tgtaacgaaa tgcgtatctt
     1141 tatgatcaac taagtaatca ccaatttctt tcggatcacc aggaacaaag ttaactacgc
     1201 cttttggtaa tcctgcttct tctaaaattc ccattaattt ataagcgata taaggtgtat
     1261 cctcagcagg tttcaataac actgtattac ctgccacaac tggtgctaaa gttgtaccag
     1321 ccataatcgc aaacgggaag ttccacggcg gaattgtaac acctgtacca attgatttat
     1381 agaaatattt attgtgttca ccttcacgat caagtactgg cttaccttga gccaagtcca
     1441 tcattgaacg tgcatagtat tcaataaaat caataccttc agctgcatca ccaactgctt
     1501 catcccatgg cttacctgct tcataaacca taattgctgc aatttccgct tttcgacgac
     1561 gaataattgc cgaaacacgt aacataagct ctgcacgatc atttgctgac catgttttcc
     1621 aagatttata agcttcgttt gctgctttaa acgcatcttc aacatcttgt tttgttgcct
     1681 ttgatgcatt tgcaatcact tgtgatgtgt ctgcaggatt gattgattta attttgtcat
     1741 ctttgaaaat cttctctcca ttaatcacta atggtatgtc ttgacctaat tctttttcca
     1801 cgtctttcaa tgctttctta aacatatcca cattttcttg gactgaaaaa tcgtaaccag
     1861 gttcattttt aaattctact accatgtaca cttaccccct ataaattttg aaagtggttt
     1921 aaccctttga tttaatgata taacatcatt taaactcatt ttactatgat taaggttagt
     1981 tttgcaatcg ctttcatttt tatgctttat cacttattct caagtatttt gaaattgatt
     2041 ggttactttt taaaatttat atgggtcgca actgctactt tatcgtttcg tcatttaatg
     2101 tttcggatgg taggtcatta tcaattttac gaacgacttt acaagggttt ccaaccgcta
     2161 agctgtgtgg cggaatatct ttagtgacaa cactaccagc accaatcaca ctgccttctc
     2221 caatcgtcac ccctggtaac acggctacat gaccgccaaa ccaagtatta ctgccaatat
     2281 gaatgggtcc ggctttttca aaaccttcat ttctatgatg gaaattaagt ggatgtgtcg
     2341 ctgtgtagaa tccacaatta ggtcctataa aaacattatc gccaattgta atctgtccac
     2401 catccataaa gtaacaattg gtgtttacat agacattttt tcctagttta acgttccaac
     2461 cataatctgt atcaaaagga atcgaaatac ttacattgtc tgttgttgtt tgaaataatt
     2521 gatcaattaa ttcctttctt ttatttgtag cactcggtct tgtatgattt aattcaaagc
     2581 aaatatcttt cgctcgtgca cgttcattga ttaagtattg atcaaagttt gcatcgtacc
     2641 atttttctgc taacattttt tctttttcag tcattacacc tttcaactcc taataactta
     2701 tttacttgtt taaaagttaa tcaaataaac attcgcctat gcaactaata cgctataaca
     2761 ttatgaaatc atgaccttat cacccttatc tatacaattc tcgcatcaaa tactgctaaa
     2821 gtagtagata aattcaatac tacagacgca ttcatttttt aatctattaa cgtacaatgt
     2881 gagtaagaga aatataaagg agtatgatag cgatgagaat attaattaca ggcacagttg
     2941 ctatcttaat cattttaggt ttggtcaaaa cgatacaaga tcacgaaatg acaaacgaca
     3001 cgagtcgtca gttgtcagac aacaaagatg atgataaagt catccatctt aataatttta
     3061 aaaatttaca tgcgaaagaa tttaacccat ctgatttctt ttaagtcacc taagaattgc
     3121 aaatccagaa gtcatttaag ttttaccttt cattcataca tcctttaata ttaattacga
     3181 cttcttttat atagatgcta aatagagaga ttgttgtgca atgtttgcac ggcaatctct
     3241 ctttttcttt ttaaaattgg taaaagtaaa acgcaacgat tgacttatat acctataggg
     3301 ggtacattag acgtgtaaca atgaatcaca gggaggcaat aatgtggcta atacgaaaaa
     3361 aacaacatta gatatcactg gtatgacttg tgccgcatgt tcaaatcgta tcgaaaagaa
     3421 actgaataaa cttgatgacg ttaatgccca agtgaattta actacagaga aagcaactgt
     3481 tgagtataac cctgatcaac atgatgtcca agaatttatt aatacgattc aacatttagg
     3541 ttacggtgtc gctgtagaaa ctgtcgaatt agacattaca ggtatgactt gtgctgcatg
     3601 ctcaagccgt attgaaaaag tgttaaataa aatggacggt gttcaaaatg caacggtcaa
     3661 tttaacaaca gagcaagcta aagttgacta ttatcctgaa gaaacagatg ctgataaact
     3721 tgtcactcgc attcaaaaat taggttatga cgcgtctatt aaagataaca ataaagatca
     3781 aacgtcacgc aaagctgaag cgctacaaca taaattgatt aagcttatca tatcagcagt
     3841 attatcttta ccactattaa tgttaatgtt tgtgcatctt ttcaatatgc atataccagc
     3901 actatttacg aatccatggt tccaatttat tttagctaca cctgtacaat ttattattgg
     3961 atggcaattt tatgtaggtg cttataaaaa cttaagaaat ggtggcgcca atatggatgt
     4021 acttgttgct gttggtacaa gtgcagcata tttttacagt atttatgaaa tggttcgttg
     4081 gctaaatggc tcaacaacgc aaccgcattt atactttgaa acaagcgccg tactaattac
     4141 cttaatctta ttcggtaagt atttagaagc tagagcgaag tctcaaacaa ccaatgcgct
     4201 tggcgaatta ttaagtttgc aagctaaaga agcacgcatt ttaaaagatg gtaatgaagt
     4261 gatgattcct ctaaatgaag tacatgttgg agatacactt atcgttaaac caggtgaaaa
     4321 gatacctgtt gatggcaaaa ttattaaagg tatgactgcc atcgacgaat ctatgttaac
     4381 aggtgaatct atccctgttg agaagaatgt tgatgatact gtaattggtt caacgatgaa
     4441 caaaaacggt actattacta tgacagcaac aaaagttggc ggggacactg cgttggcaaa
     4501 tattattaaa gttgtcgaag aagctcaaag ttctaaagcg ccgattcaac gattggcaga
     4561 tattatttct ggttatttcg ttcctatcgt tgttggtatc gcactattaa catttatcgt
     4621 gtggattact ttagttacac caggtacatt tgaacctgca cttgttgcga gtatttccgt
     4681 tcttgtcatt gcttgtccat gcgcattagg acttgctaca ccaacttcta ttatggtagg
     4741 tactggtcgc gctgctgaaa atggtatttt atttaaaggt ggcgagtttg ttgaacgcac
     4801 acatcaaatt gataccatcg ttttagataa gacgggtacc attacaaatg gtcgtccagt
     4861 cgtgacagat tatcatggtg acaatcaaac gctacaacta cttgctactg ctgaaaaaga
     4921 ttctgaacac ccattggcag aagccattgt caattatgca aaagaaaagc aattaacatt
     4981 aactgagacg acaacattta aagcagtacc tggacatggt attgaagcaa cgattgatca
     5041 tcaccatata ttggttggta accgtaaatt aatggctgac aatgatatta gcttgcctaa
     5101 gcatatttct gatgatttaa catattatga acgagatggt aaaactgcta tgctcattgc
     5161 tgttaattat tcattaactg gtatcatcgc agtggcagat actgtaaaag atcatgccaa
     5221 agatgcaata aaacaattgc atgatatggg cattgaagtt gccatgttaa ctggcgataa
     5281 taaaaacact gctcaagcca ttgcaaaaca agtaggcata gatactgtta ttgcagatat
     5341 tttaccagaa gaaaaagctg cacaaattgc gaaactacag caacaaggta agaaggttgc
     5401 gatggttggt gacggtgtaa atgatgcacc tgcattagtt aaagctgata tcggtatcgc
     5461 cattggtaca ggtacagaag ttgccattga agcagctgat attactattc ttggtggcga
     5521 cttgatgctt attcctaaag ccatttatgc aagtaaagca accattcgta atattcgtca
     5581 aaatctattt tgggcattcg gctataatat tgccggtatc cctatagctg cattgggctt
     5641 acttgcgcca tgggttgctg gtgctgcaat ggcactaagt tcagtaagtg ttgtcacaaa
     5701 cgcacttaga ttgaaaaaga tgcgattaga accacgccgt aaagatgcct agattcctta
     5761 ataatgaagg attcgttggt gattctgaga taggctagtg attggctcta taatgtcgcg
     5821 gtttacagtt ggattttcgc tccaactgca tatatagtta cacttttcgc ttggcgaatt
     5881 agtgtatctt acctaatagc tccgcctatt aggttccatc attattataa ataataagta
     5941 cactacggtt tacagttgga tcttcgctcc aactgcataa gagccactaa ttattataaa
     6001 taataagtac actacggttt acagttggtc cttcgctcca actgcataag agccactaat
     6061 tattataaat aataagtggc tctaaaagga ggtgaatgct atgtcacaag aaattttaaa
     6121 tgttgaaggt atgagctgtg gtcactgcaa aagtgctgtt gaatctgcat taaataatat
     6181 tgacggtgtc acttcagctg acgttaacct tgaaaatggt caagtaagtg ttcaatatga
     6241 tgacagtaaa gttgctgtat ctcaaatgaa agacgcaatt gaagatcaag gttacgatgt
     6301 cgtttaatta ggcaatattc aacgtcatca acaccaaatt aaaaaatcga actgatgaga
     6361 atcccaacaa tccaaattat ctcatcagtt cgatttttaa tttactcgta acctagtatc
     6421 tccagtctgc aatacatcta atgttgcatc taatgcatcg acaattagat ttttaactgc
     6481 agcttcagta taaaacgcaa tatgtggtgt taatatgaca tcttccctgt caatcaacga
     6541 ttctaacaat ggatcgttca gtgttttgcc cctttgatca cttgggaaaa gtttgcgttc
     6601 aaattcatac gtatcaagtg ctgcaccttt aatcacacca ttgtctaatg cgtctaataa
     6661 cgccttagta tctactaaag aacctctcgc acaattgaca aatactgcgc cctttttaaa
     6721 atgtttaaat aattcagcat taaatagata atgattatat ttcgttgcag gtacatgtaa
     6781 tgtcacgata tcagcacctt caaccgcttc ctcaatcgta tctttgtaat cgacatacgt
     6841 tgcaatttta gcattaggaa acgggtcgta tgcgaccaca tcactttgat aaccattggc
     6901 aaatatatcg gctactacac ggccaattcg acctgtacca ataacagcta cttttaaatc
     6961 tttaatggat ttcgataaaa tagtaggttc ccatctaaaa tcatgctccc gcactttcgt
     7021 ttgaatttga ttaaaatgac gaaccacatt aatagcctgg ttcacagcaa actccgcaat
     7081 tgaattcgga gagtatgacg gcacatttga cacaatcaag ttatacttgt ttgctaactc
     7141 caaatcatat gtatcaaatc cagcactacg ttgtgcgatt tgtttaatat caagttcatt
     7201 taatcgttta taaacatgct ctgataatgg tatttgttgt gatagcgata agccatcata
     7261 accagcgaca ccttcaacat tgtcatcagt taatgcttct ttagtaatat ctacctcaac
     7321 atgatgtttc tctgcccacg ccttgatata aggcatatct tcatcacgta cactcatgat
     7381 tttaattttt gtcattttaa catcaccctt aactttatta ttcatataaa tatgctagtt
     7441 ctgttaatct tattgcagct tcgtctaatt tctggtcatc taacgccaat gaaattctca
     7501 cataacgatt accattctct ccaaatggtt tccctggagc aacaagtatt gacttctctt
     7561 gcactaaaaa ttgctcaaat tgctcgctgt cataaccagg cggtgtttcc aaccatacat
     7621 atatgccacc tttagcatga acaaatggca aatcagcttt tgcaagcatg gcttcgaatc
     7681 ggtcacgacg tgttttaaat acattgcttt gttcttctaa aaaatcatca taatgattca
     7741 aagcatatat tgcggcatct tgtaatgcac caaacatccc agcatttgtg tgcgtttggt
     7801 actttttcaa agcttgaatc atatctttat taccaactgc aaaaccgact ctaaaacctg
     7861 acatgttata acctttagac aaggaataaa tttcaatcgc aacatctttc ccattttccg
     7921 aagcaagtat actaggattt ttagcgtcga aaccgaaagc accataagca aaatcatgca
     7981 cgattttagt gtctgtatct ttaaatttag ctatcgcttc atcaaaaact tctttcgtag
     8041 ctgtcgatcc agttggatta tttggatacg ttaaataaat gagttttgtt ttatctatta
     8101 tttgtgaatc aactgtggac caatctggca aataatgtgg cggttctaaa ttaagcggga
     8161 caggcttgcc atcagctaaa agtacacctg ctaaataatc cgtgtagcct ggatcaggta
     8221 gtaatacata gtctcctgga ttgataacac atgttggtac tgccactaat ccattttttg
     8281 taccatataa aatgcacact tcatcttctt tatctaacgt cacattatat tgtctttgat
     8341 aaaaatctac aatagcttgc ttgaacgctt ctttaccatg aaaagcacca tatttttgat
     8401 tttcaggaat agttagtgct tgttgaaaat gatcaataat accttgtggc gtaggcccat
     8461 cagggattcc aactgccata ttaattaatg gcaatggtcc atgttcgatt ttacgtccca
     8521 tcgttttccc gaaataacta tcagggatat ttgctaattt gttagagatc atcaaattcc
     8581 tcctctatca ttaaacatag cctgggcgac tatcataatc ctaacaactt gtatcactct
     8641 catttagatg gttacaatga catcgccatt caccgttatg ttcaacagaa cttatgacac
     8701 acgttgtatt gaatgaattt attttcattt taggtaggta taatattatt gtcaatatta
     8761 ggaattttca gattaatata cactcaatca ttatgattca actgccatgc atatccgcat
     8821 gcgcaaccag ttagatatgc ttatataaag tataacgccc atcaaggtac gtattcaaac
     8881 gtgaacctta acaggcgtca ttcattgtta aataaaactt cttaagcaca tacttatttc
     8941 actatgcctt ttacgttccc cttatacttt tctcacatct ttctcttaga ctactccctt
     9001 acacgccttg ctcaatatct ttaatcattt catctacagt tattttcgca ctcgttaaga
     9061 caataggaac gcctgcacct ggatgcgtac ttgcacctgc aaaatataaa tctttataat
     9121 ctcgcgatac attttgtgga cgataataat tactttgcgc taaagttggc attaaaccga
     9181 atgccgaacc aaatttcgca tgatacgttt gctcaaaatc atttggcgta aagattgttt
     9241 ctgaaacaat atgcgatttt atatcttcaa atacttcaat cgttgctaat ttacgataaa
     9301 taatttcctt tatttgttgc gtcaaagctt catctgacca atcgattccg ctacctgttt
     9361 taagttccgg cgtcggcatt agcacataaa taccagtttt gccttctggc gcaagtgatt
     9421 tatcagcgac cgctggtaca tacacataaa tagaaggatc atatgataaa cgtccctcaa
     9481 atatttcttc aatattgcct ctaaagtcat ctgaaaaaat aacattatga agtctcactt
     9541 gatctgtcac atcaatatct ataccgatat acattaaaaa tgctgaacaa gagtaatcta
     9601 agtctgcaat tttatgtggt ggatactttt taataggtgc aaaatctggc attaatgatt
     9661 ccgcaacact agggaaatca gccgtacata aaattttatc aaattttctt atgtcaccat
     9721 tcacttttat cgcatcggcc cgtttgaatt taggatcaat aataatttgc tcaatttcag
     9781 catttagttc aatattaacg cctaagtctt tatttaattg cgctagccct tgagccatgc
     9841 catacatacc gcctttaata aaatgcacac caaacatcat ttcaatcata ggaataattg
     9901 aatatagtga cgggcctcgt tttggatcaa ttcctatgta taacgtttga aacgctaaaa
     9961 gcttttgtat cttttcgtta tcaatataat gttcaattag ctgatctgca tgatttaacg
    10021 tttttaactt agcaccttgc acaagtgacg tcatattata aaagtcactc ggtttgcgat
    10081 acgttctttc taagaaatag cgacgtgcaa tttcatattt tttataaaca tccgttaaaa
    10141 aggacataaa accatgcgtt gaaccaggtt ctatactttc tagcatttgc tgtaattcag
    10201 ctaaatctgt aggcaccgtt atacgatcat cgtggtcaaa atacacatcg taaatataac
    10261 gtaattgtct caattcaata taatcttcat aatttttacc acacgctgta aaaacatctt
    10321 tataaacatc tggcatcatg acaattgtgg gacccatatc aaatgtaaag ccgtctttct
    10381 ttaattgatt catacgcccg cctacattat tatttttttc aaatatcgtc acttcatgac
    10441 cttgagaagc aatacgggct gccgctgcta atcctgtgac acctgcacca attactgcaa
    10501 tcttcattat tcaaccacct atattctatg atatttacta tttatttcat gaaacaactt
    10561 tgcctttttc cgcttatcca caaaaacacg ttcatgtaat gtatagttag cctgtctcac
    10621 ttcgtccagt atttcaatat atatacgtgc tgctaattct atgattggtt gtgcttcaat
    10681 actaaatact ttgatttgat ccataacatc ttgaaaatct ttttctgcga tagctgcata
    10741 atattcccat aagtcaatat aatgattatt aacaccattt tggtacactt cagcaatatc
    10801 aacttcatat tgctttaatc gttgcttact aaaatatatc cgttcattgt caaaatcttc
    10861 accgacatct cttaatatat taatcaattg caacgattca ccaagtcttc ttgcgacatc
    10921 gtatgtctga tgtgtttcat gatcacttaa aatcggcgtc aatacttcac ctactgtacc
    10981 agcaacacca taacaatatc cgaataattc agcgtccgtt tcaaacattg taaaatgttg
    11041 atctttatat acagtatcaa tgagattata gaaagattga aaggcgatat ttttatgttg
    11101 tgcaacatgc tgaagtgcca tcattattct acgatcactt tgaaagtgat gatgttcata
    11161 tgggtatttt tcaatagatt gtatatcttc ttttatttga tttaaaaatt gaatatcgcc
    11221 ataaacatct atactgtcat caattttacg acacacagca taaattgccc aaaccgcttt
    11281 tctttgatct tctggtaaca agtcaaaagc gtaagaaaag ctttttgaat gtttcttcat
    11341 gattttatga caatatttaa aattcatatc catcattgtc atactagtcc tcctatattg
    11401 aaatgccatt accttgattt attgttcttt agatatagaa tattgacgac ctttccattc
    11461 aactacacca taccgatgcg tttgtttcca agattggatg aaaattttag taaaaaacat
    11521 aaataacaat ggatgacata ccattaataa attagaaaat tggccgaccc ttcgatgcag
    11581 ataaataaat tgtgtagtat agctcaagta aagtgctacc atttttctta cagacatttg
    11641 gcgatatttt aaactaagac atagccctaa aatactcgct atagaaccaa acaaccacaa
    11701 cacaattgct gtcatgatct taggctttgt gccacctgcc ccagttgaca aatgctttgt
    11761 ccatccttct tgtaatgact gaaatccttc ttgatacatg cgaaatgcaa caaatggaaa
    11821 cccttcataa actgttacgg gcaatgattg tgaagtatat gcacttccta aagcaaatcc
    11881 ttcaataata tgacggtttg cacttttatg acctccagtt gcataataat cttctttatt
    11941 tgttaatgtc accggtccaa atgcatgctg gttagtccga ccgtcgtcta aggtagaaaa
    12001 tacattcata ccaacgactg tcattaaatt aaatatcgct gaaaaccctt cgtagaaacg
    12061 ctttgttata tgataaggct gtacgcttaa caatcctttt tcaccttgta attgatactg
    12121 attaatcaac gtttcaacag catctttcct taagaaagtt acatcagcat ctacaaaggc
    12181 aatacgattc gtacatgcat gcgtcacacc ttgataacaa gcatgtgatt tcccatacca
    12241 tttgccgtca gtatcatcaa caacatctac tacagtcgca ccatatgaac gtgctacacg
    12301 tgctgtttca tctgtcgatc cgtcattcat aacaatgaca tctactggaa cttgctgttg
    12361 tattatcgaa tgtagtaaat gacctattct tttttcttcg tttctagccg gaataataat
    12421 tgttaatgct ttatgattga agttcagcgt tttcgccttt agctgatgtc tacgattaaa
    12481 tatcaatgca ccacacgcca tagacatggt cactattact gttaatattc gtgataacca
    12541 tttcatagat atcacttcct attcttcgct tctcgcgcgt ttattttgtc tgcgacttgt
    12601 tgcccactta atgtaaccat tggtatgcca ccaccaggat ttactgatcc acctacaaag
    12661 tacaagtttt caaaatactg actttcttta ggaaatttaa atcctttgtt tttcttttta
    12721 tctgctacaa caccatatat tgcaccacga ttagaacgat aatttttttc aatatcctcc
    12781 ggtgtccaaa catcttcata aataatgtgt tttcttaaat cagtaagtcc cattttttct
    12841 aatttatcca aaattttatc cctaaacttc gcataatctt cagttgtaaa aggctgatct
    12901 tgaatatatg gaatatgtgg taagactttg atattttcat aacctactgg cgcttgtgtg
    12961 tgatcagttt tattcgtatt tactagataa atggtcggat catctggcaa taccttttca
    13021 tgaaaaactt gttgataatt gagataagca ttttccgtaa aaaagaaatt atgatgtgct
    13081 aattgcgggt attggcaagc aacacctaaa tgcatcacat atcctgagct tgccggctca
    13141 aattccctct ctaatttgtt taatcgttga gtatcaaggt gaattaaata tttataagta
    13201 ggtatgactt ccatatttga aataatataa tctgccttta caaactcacc tgtatctaat
    13261 ctgacacccg tcacacgtct ttgatatgtt ttaatattgt ccacacgtgc acctgtatga
    13321 attgtgacac cttcttcacg cgctagcttt tccaaggcat tggctaaatg atggattcca
    13381 ccttctacat accaaaggcc ttgctcttgt tgcatatgga ataacataga taatacagct
    13441 ggcgcatcgt atgatgaaga acctacatat ttgataaaat agcctaacat ttgtcgcaag
    13501 tatggattcg agatacgctt gtctatggcc tgttgcatag tatgtacata atcataatta
    13561 ataagagcat ttaatggccc atgaaattta ataatttgag atagtgtatc taaaccatag
    13621 ttaaaatacc ctttttcagt aatacgatcg attcgtcttg tataattcaa ataattttgc
    13681 agttcctcta tatcctgttt cgacaatatc gcattatgct gacctgtttc tttaatacct
    13741 tcatacaaat cgatagtcgt tccatctgga aaaaagctac gccattgatg tggcaatcgc
    13801 ttgattgtaa cgtagtctga catttgcttc ttgctatatt cgaataattt ttcaaaaata
    13861 taaggcatcg ttaaaataga tggacctaaa tcaaagccaa agccatctga ttcatgacga
    13921 ttcactttgc ctcctatatg attattttgt tcatataatg agaccgaata gccactttgt
    13981 gccattcgaa ttgctgcaga aatcccacct aagccaccac caataacgat gatatgttta
    14041 gtcatgacgt tcacctcttc gttttaatat gcgtagtaac tgcgttaatc tcggtcgatt
    14101 atagcgttgc acaataatga ttggaacatt agctatcatt gcatagaaaa tatttatata
    14161 ctttactaaa cgagggcctt tattgaatat gatgactgga agtatcgata tccaatgaat
    14221 caactctgcc ctctttgtct ctataatcat tttttcaatt tcatcagtat tgattgtcat
    14281 taaatgacgc tgatcatata tattttgatt aagctgatga ccatctaaaa tcttatgttt
    14341 ccattttctt acgtaaaacc atttgttcca aaattttcca tgcttctcaa agttaaatga
    14401 tttaaatatt atgtacttct gacgaaaata cttatcagga attcttgtac ctaaatttgc
    14461 tatatttagt tgaacaatta accaatacat actacaaaaa aatgctgttt taatatattt
    14521 tttcatggtt ttcatctaaa ttgaatcact ctcaatcata ctgacatagt ataaattatc
    14581 actttattac ttagttccct aaagatcagt gaataaaccg ctctcattta tatttatata
    14641 aaacaaaaac gccaatactc tcatgttaga gaatattgac gtcatttgat ttacatttga
    14701 ttaatgaatg aaattatatg aacctgcttg gtttgctgaa attgtacgag acgtaaccac
    14761 accagcacca tgtccatagt tcatttctga aacacgaaca gaaccgttgc tgttaacgcc
    14821 ttcaacgtaa gcaacatgac cgtaatagcc ttgtgttgtt tgcatgatag caccaacttt
    14881 tggtgtattg ttcactgtat agccagatga tgcagctgcg ttagcccaat tacttgcgtt
    14941 accccatgtt gaaccaattt tcccaccaac acgatcaaat acataataag tacattgtcc
    15001 tgaagtatat aagttacttc ctgatgcata accattagaa attgaacgac catttgaaga
    15061 tggcgctgca gttgtagtca catgaacatt attacttgtt gtgctataac ttgcacctga
    15121 accaccagta taatagttat ttgttgctgt ttgactattg tttgtatagt tattgtattg
    15181 atatgaatga ttatagtagt tattgtaaga tgcattgtta taactataag tattgtagtt
    15241 gttgtagtag tatgtgttgt tttgcgttaa ttgacttgga ttccaatttc ctgtccaagt
    15301 gtaatgataa ttaccttgtg catcaattgt atacgtatag ctgtatgatt gagcgtcatt
    15361 agaattatat ccattgttat tttgttctgc ggcttgtgca tcatgtcctg caaatgcgat
    15421 agtggcaagt cctgctgtag cgattgtagc tgtaacgatt tttttcataa ttaaatatcc
    15481 tcccaataat caaaacactc tatacatata gtcattagcg actcaattat tttaaatttt
    15541 atatttttta tttttttagt atgcaaataa ttataacttt tatagttttg cgacaaaaca
    15601 aactataaca gagattaggt gtcttgcggg ctttgttatc ataatgtaat ctgttcctaa
    15661 taaagcttca ttgaatgtaa tataccaatt agaatgttta caacactttt tctgcaccgt
    15721 taccatggga tttgaaaata aaaaagcact aaacttttaa tgaaatacat catttttgat
    15781 aataaatatc agaagtaatg taatatttca aaagtttagt gcatcaaatt atttcttatt
    15841 tgtagcatgt gtttccatcg tttttacaat caaatcagtc agcgctttac tacctgcata
    15901 ttctaagtga ataccgtcat atgcaaagta ttccggatga cctgcagaag ctttatacca
    15961 atcgactaga tgtacattag agcgcttttc agctgcctca taaattaatt tatttattct
    16021 accttcataa tctctaggta ctctaataga aactaaataa atgtctgctt ttccaaaact
    16081 atccaatagt tcatttaatt gatctttcgt aaatgcccca tttgtaccaa gctctactac
    16141 aactttttga ccttttttag catagtcttt atattgcgat ttcacaattg gtgtagcatc
    16201 aacgagttgc cgtccaactt taccatcaat ttgtgcattt ggtattttct tagtaaagac
    16261 attaccaata tccaccatga ccgagtcacc aattaatagt ggtgatgact ttttaatatc
    16321 ctctttgcta ttcgctgtct gcttatcctc ttgcttatct tttttaacga cttttttctt
    16381 cgttgtttta aattccgtct tcttctcttt cttctcggct tcatgttgtt tgccaagtgc
    16441 atcaaactgt ccactgagca caacgataga cggaaccaat aataggataa ctaacactgt
    16501 tctagcaaat tgccccttct ttttaggtaa aaatgcaaaa gctttaaatc cttttttacg
    16561 tataggtgtt tcaataaagc gatacgaaat ttcagccatt aacgctgtta acaaaatttc
    16621 tataatataa acgtataccg gtatttgtcc ttgtacgtaa taactgttca caaaaacaat
    16681 gataggataa tgccataaat ataagctata tgatcgttta cctataatta gtaaaggttt
    16741 catacttaaa aatttagcaa ataaactaga aggatgtacc gcaattgcaa taatgaataa
    16801 agttgcaaat gatataatgt aaaatcctcc attatagatc cattgatctt ggtcgccaac
    16861 tataaagaac aaagtcatta gaaccgcaaa accagatatc cctataatat ctaatgatac
    16921 gacaatcttt ttagaaatat cttttttcaa agcaaacgga ggccaaataa atgctaatat
    16981 acaaccaagc aataaagttt gcagtcgtgt atctgtccca aaatacacac gtgaattatc
    17041 tccagtgatg aaatgaatca ctatcataag tcctaaagaa atcaacgata caataaatag
    17101 cgtttgaata atatttctcg gtttaaatct atgtaataag aacgtgataa ccaatgggaa
    17161 aagcaagtaa aattgttctt caatggctaa agaccataaa tgtttgagtg gttcaatagc
    17221 aaattggtta aaataatcta cattctgtga gatgtaccac cagtttgaaa catagaatat
    17281 agctgcaata gcatctcgtt tcatttgtat aatcaattcc ggtttaaata tcagtgtgaa
    17341 cgtaagcacg acacaaatta aaaacaacac tgccggaatg agtcgtttca atcgtcgctt
    17401 ccaaaactct agcaaatcaa ttttttgcgt ccgataatac tcacttatca acaaacttgt
    17461 tattaaataa cctgaaataa cgaagaatgt atctactcct aaaaagcccc cacttaacca
    17521 ttgtgcattc aagtgataaa taatgattcc tataactgcg aatgccctca acccatctaa
    17581 tccgggtaag tatcgcgggg aatacatttt ttctaaacgt ttaaagtctt ttgtatccat
    17641 gttaataaac gccccattta tttttctcta ttttgtagca tatcacaata tttttgaaaa
    17701 taaaatattg cactgatttt cattaattga tttaaccctt aattaagata gttttaaatt
    17761 ttttattaag tagaaaacaa ttattacagt taatttcatt actgcaaacc acatataaat
    17821 ttgtcgattt tactacataa catagattat catagattct tgaattttta gcaaaataac
    17881 tgttattttc attatatttt tacaaaaaaa aggttcgttt tatattttat gcctcttact
    17941 gtaacagaat cattaagata tgctattcga atatactttt tcaaaattta tataatgaat
    18001 aaattaacat gtattgaaaa aaaagcgaaa tgcagcctat cctctaatgt aaaccaaacg
    18061 atatatctcg tcagacttta tatttaaacg ctatgtgtca cttttaaaat gaatattact
    18121 aagattgtca tatcaattat tattgcatcg aattaatctt ttaaatttct gtaataaaac
    18181 ctattatcta catctaattt aggaaaacta cttggtaaat ctcctcgctt aatttcacgg
    18241 aagccattat tagaataaaa atactgtgca ctaataaatt tatcaattgt tcctaaataa
    18301 ataccatcga tattttgttc tttacatgtc attataactt tatctaaaag ttttttacct
    18361 atttttaaat tcctataacc tttatcaaca aacatttttt taagtgcaga catattatta
    18421 tctagtctaa tcaaacctat agtaccaaca atattttgat gattgtttat tgcaagccaa
    18481 aattgccctc cattattcaa atagttatgt tcgatgttct ccaaatcagg ttgatcatct
    18541 ctatcaattt ttatattaaa ttcatttttt tgaatcgata aaataaactc gattagctct
    18601 tccttataag acctattata ttcaattatg tttatagcca tttttatctc ctttttcatt
    18661 taatttaatt ataaaatgtg cgtttagttt gtatctagtg tactcagtac agcctcaaat
    18721 gaagtttcat tccacttggc acttaataaa gacaagtatt ttagcagtaa tacaataaag
    18781 tccaataaat ttccctaact tcaatatcca ctttttaaaa aatgtatttt taattaataa
    18841 aaaaactctc cccaatttct atgggaagag ctatatattt aatgtctaaa cattactttt
    18901 atttattatg aaggaattag aatccccaag cacctaaacc ttgtgctttg tatgctttaa
    18961 cagctgcgtt gatttgttgg tcaacagtgt ttgttggacc ccaacctggc atagtttgga
    19021 ataaacctga agcacctgat gggttgtaag catttacttg accatttgat tcacgagcga
    19081 tgattgcagc ccatgtagaa gctgaaacac cagtacgttg agccatgatt tgagctgctg
    19141 atgaaccagt agcacctgca gtattaccat tgcttaatct cactgaactt gaagtagttg
    19201 aagtgctgta gttatggtaa gttggagctg aaacagcttc aacgtttgag ttacttgatt
    19261 gtgcattgta gcttactgat tgtacatttg aaccttggtt gtatgaagta gtgtagtctg
    19321 cacctgcaac gtttgagaaa ccagcagttt gaccattagc tgcttcatag ctccatgacc
    19381 atgtagtacc atttgaagtg aagttatatt ggaaaccatc ttttacaaag tggatgtcat
    19441 atgcaccatc tttgattgga gctgcattta attgatcttg gtgattgtgt gctaagtcaa
    19501 ctaagtgtgc ttgatcaacg tttacttcag cagcgtgtgc ttgatgtcct gtacctgctg
    19561 cgtaacctgt tacacctaat gccactgcta atgatgatgc cataattgtc tttttcatag
    19621 taaaaaatcc tccagtaata attgtaagtt tatgttttta gtaattatat tttgaatttg
    19681 aatgtcgtag tgcaagttta aattgtcttt tatttctttc aacggtactc actatatcac
    19741 aaaaaaccag ccagtaaatt acactttctt tacaaaacat tacaatatca agttttattt
    19801 gtaatgttga aatatggctg ttttatactg taatgtgaaa tatgtgccct ttagaatcca
    19861 ataaaccctt gaaatagtct ttaacgcata agatttttac tatatttagc tcaactatta
    19921 cagctttcgt aatattacag attgtatttt tgttacatag ctgtaatata tctgacatgt
    19981 aacttctctt attttcgtac attaaacgca attaaaaagc aatcaacaca tatgtttcta
    20041 cacatgtatt gattgctatt attgttgtat atccaaagtt ttaaaacaca catcttttgt
    20101 gaattgtctt atcttttata agcacaaata aactgcacct taattatatt gctaaactct
    20161 attatcgtaa ttcacaataa cattaaataa tttttgatct catattttca aaaaacatac
    20221 tgttattatc ccatgaattt aaaaatatca ttagtatata aacgaaacac tttacgataa
    20281 atgatatctg caagccaagc tgttacaaat ggtacaacaa agaacgctac tacaattagt
    20341 aagacactca accaagcaga atcaacctcc ataaatttaa atgcattaat cggtcctacc
    20401 attcctataa aaccaaatcc agctgactct ttcgttccat gaatacctac taatgctgat
    20461 accaaacctg atacaatggc tgtcgttaat attggtaaca taagaattgg atatttcacc
    20521 atattaggta tcatcatttt aacgcctcca aagaagacgg ataacggcac ccctaaacga
    20581 ttcactttac ttgtaccaat tatcaatact gcttcagtcg cggagatacc aattgaagct
    20641 gatccagctg ctaaacctga aatacctatc gcaaaggcaa tggccacagt tgatagtggc
    20701 gaaataataa taagactaaa taccattgaa atcaaaatac tcatgacaat cggttgtaat
    20761 tctgtaaaac cattaaccat attaccgatg gctgttgtaa tcattttcgt atacggcaat
    20821 attaaaacac caattgcacc tgaaataccg ccaacaactg ttgggaatac aatcaatgcc
    20881 atactaccta cgcgatgttg aataagtaaa atgaacaaca ctgcaatcgc tgctgtaatc
    20941 attgtattaa ttaaatcacc aatacccgta atcatccaag caccattttt aaactgcgct
    21001 gcaccgcttc ctacatatgc tgcactcgcc acaacagcaa ttgctaatgg cgataggtca
    21061 aatttcatgg caaccaatgc accaatcaaa gcaggtactg taaattgaat tgcaacgaca
    21121 acgcctaata acgttttaaa aatcggatga taatccataa agtatttaaa aatttctcca
    21181 agtatcgcat taggaactaa acccgcaaca atacctatgg cgacacctga taaaactcta
    21241 aatataaaat ctttgggtgt aattgtttta attgatgtca taatatcatc cttccattta
    21301 tgtatataca tctttatgca aataataaag agccttaagt tataagctgc cactagctta
    21361 aattctaaga tgtgcatgcc gatgttgtta tatttaggct agcagtaaca tctataactc
    21421 aagactatga aaaatagtat atcgcaaaat tctgaatttt tagataaaga aattggcaat
    21481 ttttcaaaca tattgttaca atacactttt attttatctt catttttaaa atccattaat
    21541 acaatagaag aaagacattc aaatgcttac caaaaaggta cattatttgt taggagcgta
    21601 tcagcactta tatatcatca acacaattga caatataata gaagatactg ataataagtg
    21661 ttaaaacaac agatgttagg tagtgaacaa atgatggaaa gtaaatccat agatccaaga
    21721 atcgttagaa ccaaacaatt gcttgtcgat gcttttctta aaatttctag agaaaagaaa
    21781 ttatctcaaa ttacagttaa agatatcact gatattgcta cgctaaatcg tgcaacattt
    21841 tacgctcatt tcactgataa agaagacctc ctagactaca cattatctgt aaccatttta
    21901 aaagacttga atgataattt gagcatttct aatgtcatta atgaaaaggt tctgcgtaat
    21961 attttcattt caattgcgag ttatatcaaa gatgctgcaa agtcttgcga attaaatagt
    22021 gaagcatttt gcaacaaagc acatcaacgt attaataatg aattagaaga tatttttgcg
    22081 attatgttag aaaacagcta tccggagcat caacgagata tcattgtaaa tagtgcgagt
    22141 tttttagcag ctggtatctc aggcttagca ttacattggt ttaacacgag tcaagagaca
    22201 gccgatgtgt ttatcgatcg caaccttcca tttttaattc atcatatagc acatttttaa
    22261 taaaacttgg tatttagtca tgcatcttga aatcactatg tgacttaggt tcatacttgt
    22321 acacacaata aaatttaacg tattacgatt gattagccgt gtctaggaca taaatcaacg
    22381 tcctatactc tacaatgtca tattagcagt cgttaactga atgaaaataa gcttgtcatt
    22441 aaaacatata gattttagtg acaagcattt ttgtttttgc gtacttaaac aacacttcag
    22501 acaatatgtt gtttaggcaa caaatgatat gtgcgtgttt attggcaaac gtacaacata
    22561 gtagtatagt atgtctaaac aacacatgtt gcatagttga tatacgttgt ttaaatacta
    22621 agataggagg gattgacgtg agcgagacag atgaacctca ggggtttgaa cgcacgcata
    22681 atatattaaa tattaatcag aataggcaga gtctaatgac attcattata agtaaattga
    22741 agtcgacgtt gaagcaacgc ataataaatg ctcgtggtaa aaagcgaatc gactatcgac
    22801 tgtcgtataa cttttacatt acgtattatg atactgtaga aatcaagaaa atcgactgtg
    22861 aatataccta tgctatgccc attgcaattt taataagaca cacgatgtca ttcgacaatg
    22921 ctcatttctt tgctcagtta cgtcatcctg tcttataaaa caacattgca gacatgtata
    22981 tcaaacgaca cttcaataac atcactttgc catcgtacta ctattaaaat cgtgtctcaa
    23041 accccttatt ttaattccaa aaatctgctg gtcaaaagac cgagaaacta aaaacattac
    23101 tggatgtgtt gataaattat cttatagaaa taaactcaaa atatatcaac acttgattct
    23161 aaggaggata tgacaatatg aaaattttag atagaattaa tgaacttgca aataaagaaa
    23221 aagtccaacc acttactgta gctgaaaaac aagaacaaca tgcattgcgt caagactact
    23281 taagcatgat ccgaggacaa gtattaacaa cattttccac aataaaagtg gttgatccaa
    23341 tcggtcagga tgtcacacca gataaagtgt atgatcttcg ccaacaatac ggttatattc
    23401 aaaattaata tttgctcacg aggtattgca cttaaggtgc caactgacct cataaacaaa
    23461 gcccatactg attgaagaca ctaatgtgtc caccatgtct cttacgacat gtgcacatta
    23521 aaccttcatc tttgtatggg ctttttattt attcttttga gaatttcatt ttagcagacc
    23581 aaaaaattaa aatggtgtcc atcaagatct ttgaacattg caccataaaa cccttggctt
    23641 actgttggtt cttgaacgac tgtgccaccc gcttctttca ctttattcac caactggtca
    23701 acttcttcat ttgtgttcac acctagagaa atcatcactt catttggttc agtattaagg
    23761 ctttgctgac ttacattttg aaaatgcttg ttttctatta aaattacggt tgtttgacct
    23821 atttgaatgc cgaccatttt atctaacatt tgtgggtttc tatttatttt aaatcctaac
    23881 gctttataaa actgtgcgct cttttctaaa tcttgcacat gcaaattaaa ccacattgat
    23941 tgaatcatga ttgcacccca ttcattactt attatagttt tggactttaa gccaatcact
    24001 taatgataat cttgttggat ttatttcagc cattaattca aagtctactt cataaccttt
    24061 ttcttccaac cattgctttt ctgcaacacc actaacaaat tctccttcta taacagtaga
    24121 tttacctgtc acttcactaa aaattgttgc tgcttcactt aatgtaactt catcggaacc
    24181 aatctctatt gattgatgcg taaagctttg tggatgtgca aaaatatacg atgcaatttt
    24241 agctatatca atagaagaaa tcattgtgaa ttttatattc ggattaataa attctggtaa
    24301 tgtaatacgt tcatcttcga ctttagcaat gcgtaaaaaa ttatccataa agaatgatgg
    24361 tttgataact gttgcattta tattagattc cattaatcta ttttctattt ttgctagtac
    24421 ttcaaagtgt gggccagttc gatttcgatt aacccctccc gcagtactat acacaatatg
    24481 ttgaatattt tcttgctcag ctatttcaat tatcttcata ccttgtctta attcttcgct
    24541 aacatcatct ttaacgattg gctgaatact gtataagcca tacttacctt tcatcgctga
    24601 ttgcaaacta acattatcac tcagatcacc ttcaacgatt gataaatgcg gatgtcctat
    24661 gtctgaaagt ttacgattat tcttatttct agttaatgca cttacatacc atccatcctc
    24721 taacaactgt tttacaactg cattaccttg cttccctgtt gcgcctatta ctaaaatatc
    24781 tttcatttta gatccactcc tctttttatc ttaaaaacca atagtcctct ttttcacctt
    24841 ttaaatacgt atatggttct ggtgtttgtc catttccttt aatattgtcg tttattacat
    24901 ctctgacatc ccatactaaa tgaataaatc gttgtaattg atgactaaaa atgcctagtg
    24961 gcgctgtcat agctaatact ttttccacat cttctgtagt gacattcagt ttttcagcaa
    25021 tcatttgaac cgataaggta gattcttcaa aatgtgcttg caactcttcg cacgtaatgc
    25081 gtctttgctc tttatttaaa gacatatgca acactctttt cttttttaaa tatttacagc
    25141 actttgcaaa ttcattgcta tctttcttaa cacgttttat aataaacgga gagttatccg
    25201 tttgtcaatt ttagagaatt ttaagtaggt gtaatcatgc gaaaagatgc aaaagagaat
    25261 aggcaacgaa ttgaagaaat agcgcacaaa ctttttgatg aagaaggcgt tgaaaacatt
    25321 agtatgaatc gtattgctaa agaactaggt atcggtatgg ggacattgta tcgacatttc
    25381 aaagataaaa gcgatttatg ctactacgtc atacaaagag accttgatat ttttatcact
    25441 cattttaagc aaattaaaga tgactatcat tctaattatg aggttatgca agtgtcgtta
    25501 gattatcttt tacaatttaa aatcgataat aaggcactac tgcaatgtat tgaagcaggc
    25561 aacaataaat tacgttttta ccaaagcgca ttttatcaag aattatttga tttttattat
    25621 gacttattta aaagcgatga cgacacttac acgaagttca aaacagatat gctgttgcaa
    25681 tcattatcaa ctagtgtatt tgccttccaa atagagcatc gccatatttc aattgaagcg
    25741 tacagaaatt atttattaaa catttattta gatgaggtgg aacgtaatga ctaaaattgt
    25801 gttaatcaca ggaggcaata aagggttagg ctatgcaagt gcagaggcac ttaaagcatt
    25861 gggttacaaa gtttatatag gttctcggaa tgatgtaaga ggacaacaag catcacaaaa
    25921 attaggtgtt cattatgtac agttagatgt tacaagtgac tactcagtca aaaatgctta
    25981 taacatgatt gctgaaaaag aaggccgtct tgatatcctc attaacaatg caggtatatc
    26041 tggtcaattt tcagcacctt ccaaattgac acctcgtgac gttgaagaag tatatcaaac
    26101 gaatgtattc ggtatcgtac gaatgatgaa tacattcgtc cctctcttag aaaaatctga
    26161 acaacctgtt gtcgtcaacg tatcaagtgg tttaggttca tttggaatgg ttacaaaccc
    26221 cgaaacagct gaatctaaag tgaattcatt agcttattgt tcgtctaaat cggcagtaac
    26281 aatgctaacg ctacaatacg ctaaaggatt acctaacatg caaatcaatg ctgctgatcc
    26341 aggcgctaca aatactgatt tagttggtga ttttagtaat aattccaaac acgtttctga
    26401 aggaatcaag ccaatcattc aattagcaac gattggtgca gatggcccaa caggtacatt
    26461 tattaatggc gatggagaga tgccttggta gatcaatttt atgttacaaa cattgaaatt
    26521 agaggtatat aaaaaatgct tctgcaatag atgcaataaa catgcaatac aatatggagg
    26581 cgaagtaaat gaaaagtatt acgtttgaag aacattatgt cattgaagat attcaaaaag
    26641 aaacgatgaa tgcgatatca gcagatccta aaggtgtacc gatgaaagta atgttagaag
    26701 gccttgaaaa aaagacaggt tttacaaatg ccgacgaatt atcacatcat gatgaacgta
    26761 tccaatttat gaataatcaa gacgttcaaa ttcaagtctt atcttatgga aatggttctc
    26821 cttcaaattt ggttggtcag aaagccattg aattatgtca aaaagcaaat gatcaattgg
    26881 caaactatat tgcacaatat cccaatcgct ttgtaggctt tgcaacttta cctatcaatg
    26941 agcctgaagc cgcagcgcga gaattcgaac gttgcatcaa tgatttagga ttcaaaggtg
    27001 cgctcattat gggacgtgca caagatggtt ttcttgatca agacaaatat gacattattt
    27061 tcaaaacagc tgaaaattta gacgtaccga tttatctaca tcccgcgcca gttaacagtg
    27121 acatttatca atcatactat aaaggaaatt atcctgaagt aactgcggca acatttgctt
    27181 gttttggtta tggttggcac attgatgtcg gtattcatgc aatacatcta gtattatctg
    27241 gtatttttga tcgttatcca aagttaaata tgattattgg acattggggt gagtttatcc
    27301 cattcttctt agaacgtatg gatgaagctt tattcgctga acatttgaac caccctgtaa
    27361 gctattactt taaaaataat ttttatatca caccgagtgg catgttaacg aagccacagt
    27421 ttgatttagt caagaaagaa gtaggtattg atagaattct ttatgctgct gattatccat
    27481 atattgagcc tgaaaaatta ggtgtatttt tagatgaact gggtttaaca gatgaagaaa
    27541 aagagaaaat aagttataca aatggtgcta aattattagg tttatcagct aacaattaac
    27601 aaatggaggt cattgaatat ggaaacttta gaattacaag gcgctaaatt acgataccac
    27661 caagtcggac aaggacccgt gctcatcttt attcctggtg caaacggtac cggagacatt
    27721 tttctgcctc ttgcagaaca gttaaaagac cattttactg ttgtagccgt tgatcgtcgt
    27781 gattatggag aaagcgagtt aactgaacca ctccctgatt ccgcttcaaa ccctgacagt
    27841 gattatcgtg tcaaacgcga cgcacaggac attgccgaac ttgcaaagtc attaagtgat
    27901 gaacctgtct atatattagg ttcaatcgtt gcgatgcatg tgttaaaaga ttaccctgaa
    27961 gtcgttaaaa agattgcatt tcatgaacca ccaattaata catttttacc agacagtact
    28021 tattggaaag ataaaaatga tgacattgtg catcaaattt taacagaagg cttagaaaaa
    28081 ggcatgaaaa catttggtga aacattaaat atcgcaccaa ttgatgcaaa aatgatgtct
    28141 caacctgctg atactgaaga aggacgtata gaacagtaca aacgtacgat gttctggtca
    28201 gaatttgaaa ttagacaata tacgcattca gatattactt tggatgattt cacaaaatac
    28261 agcgataaaa tcaccctact aaatgggaca gattctagag gctccttccc acaagatgtt
    28321 aacttttata taaacaaaga aactagcata cctattgtag atattccagg cggtcactta
    28381 ggttatattc aaaaacctga aggctttgct gacgtattat taaatatgtg gggttaatct
    28441 atcgttctct aataaaacag cttggacagt tttcaaatta tatttgatgg ctgaccaagc
    28501 ttatttttgt ttacgtttaa aagttcaaat caactcgtta ttatacatga aggcatgtta
    28561 accattctct tccactgaaa acactactgt tccaaacatt gatagtcttc ttttgaaata
    28621 ccctttccga taagcaccaa atattctggc actttttttg aaaatgctac aggtgttaat
    28681 tcatattgtc cttgtgtaaa ttgaatgaga tacgtatgtg cggtgtcttc aaatgtcata
    28741 aaccctttca acctataaat atgagacgga aggcatgcta aacgttttat aaactctgct
    28801 ttagtacatt tggctggtga ttgcacaaat tgatgattaa tcatttgatg taacgactta
    28861 tgtttattgt cagaagttgc tgtcatttgt ctaactgata ttggcaaagt gacgctgcca
    28921 tgcataccga cttgtatatc ggcctctggg ttaataactt ctaaatcttt caatagtttg
    28981 ctcgttgttt caacatctgc tgaatctatt ttattaacaa ataagacaga acaatatgct
    29041 aattgctcat aaaataagcc ttggatgtct ttagggaatg atttaatgtg tttatacatg
    29101 cttgcgtcta ttacaccaat catatgtgta attgttgtaa acggagctaa aataggcgtt
    29161 aaacaagcat ctaagacaga gactggttct gcaatcccac tacattcaat aaatacaatg
    29221 tctggttgct cttttaaata taattgatgt aactgttctg atacatctgc tttcattgcg
    29281 caacagatac acccctctgt cagttcactt aaaggtatgt cttcagatac taaggcacca
    29341 tcaacactca ttttcccaaa ttcattcatg atgactgctg gtttcagcga ttcctttaat
    29401 gattcagcga gcaaatgatt taagacagtc gttttaccgc cacctaaaaa cccacctata
    29461 ataactattt tcatctgcga ctctcctctt atacttcata tgaatagcat attccaagat
    29521 agatctccta ttgactgaca tgactctata ttcagttata gttcattttt ttaaataatc
    29581 agcattacta ttttaacaat gatgcctaca ccaataacta aagacaatga tgtcaccatt
    29641 tgctttccaa ttaattttag tgctgactga ccacctaaat gtttcaaaag catcgtcatt
    29701 gtgaccgagc acactgtaaa agttgaacta aaaaacacaa gtagtagtaa ctgtgttgct
    29761 gtcattcctt gtaataaggc gccctgatgc aaattaaaca agagcatgcc gtcttttcga
    29821 atcattgaaa ataaaatccc tggtgacaat tctgacgaga tgcctaataa cgataatata
    29881 ggtgtaaata tttgtgataa aacattcaaa attggcgtta gtgataaaat actaacaata
    29941 agacagattg tgataaaaat aggtagcgct tgaacgataa acatttgtat attttgccac
    30001 atttgcagca acatttgacg tatatttggc atatgtaatt gtctatcata aggtagcgga
    30061 acgctaagtt gttgatcatt ctttttcaac catatcctat tatgtaagat gccacctaaa
    30121 agtactaata ttaagtacgg cataaatagc catgactttc cagctacact aaaaatagat
    30181 aatgtcgcac ctatttgata actacaagaa ctaccaaagc ttattaaact catacactgt
    30241 gtcttcgtgc aggtatggca ttggtgtgct gcttgtgaaa tagctgctgc attacatcca
    30301 aatccttcta atagtggcac gatatcatta ccttgtaatc cgatccataa cattgacggt
    30361 tcaattgccc atatcatcca tgatttgagt cctgtttgat caattattgc agttgataaa
    30421 ctaattaata ttacaaccgg caatgcccat acgagcgaat atgttccaag tgatagcaca
    30481 ccataatcac caaataatat atgctgtaat atatcgtgat tgagcaatgt tgtttgagtt
    30541 aaatattcta tccatgctga tacatactga ttttgaaacc aatctgaaaa ttgataagcg
    30601 ataccgattg gtaatgcaaa cattaaaaat atcaacaacc aggctaaaaa tgtcgcactt
    30661 attttaggcc ttaaatgtcg tcgctcatgt tgaaatagtg tcaattgttc tatcacatgt
    30721 tcgctcgcta taggcgaaga aactacactt tctgccaatg aaatatctaa cttctgtttt
    30781 atgattaata atcttaattt cgctttttct gttgcggtaa gtttatccgc atgactaata
    30841 actaaataat gaggttcatt aggtatgggc gtataatgcc cagttctcat atcatatttc
    30901 acttctaaca tgtgcatgac acttcacaac atgaataatc atctaaatac atttgatttt
    30961 tttgataatt ttcaatgaca tcttgtttag ctggtaagcc ttcccgctgt gttaaaagat
    31021 gggcaagtac tgcaaatcgc gctctgaatt tatagatata gcataatttg gcattatcat
    31081 tttcaactgt tgcaccaatc ataaaaatat tcggataacg tgtcgattca tcatgtgttg
    31141 ttaatttaat atcttgattt gttgtcacaa ataattgttg aacgattgga ttttttgttg
    31201 catcaaagcc agttgctagt attggttcat gaggcgtatg cacactttgt ccgctatcaa
    31261 aactgatatg atactgtcca ttgttaaaat caatatcttt aactgtataa tgtacattca
    31321 tttcgatgcg agcaccttgc ttaatgacat tacctagtcg ctgacgtgta taaggtgaca
    31381 atctaacact aggatcagca tccggatcat ttaaaccggt tgtgctagta taaagtgcga
    31441 tgtcagagcc attttttgca agttgatatg cagcatcaaa gccactttca ttacctccga
    31501 taaccacata ttgcccctta ttaaagttat caaagtcttc aatttcacta taatgaatac
    31561 catatttaaa tggcttttta gggaaattat aatcacctgt tgcgacaaag atataatccg
    31621 cgtgatatat ctctgttgtc gttgcaatcg tataatatgc atcatctaca gatatatttg
    31681 tgacaactgt attttcaaag atattcagct cgtaatggtt ggcaaccact tgtaaatatt
    31741 cagcatatgt ttctccggaa atatgttctt cattaaatgt aaatgctggt gaagtatcca
    31801 tggaaattgc attcatatca ggcatgccaa atccattaga cgtaaatgat ggcgtgatcg
    31861 tacgagtcga tttcggccaa tgtttaaatg aatgtcctac tgttcctttt tctaaaataa
    31921 tgacatctgt tataccgaaa tcttttaagg taatggccat acctatacct gcagcaccgg
    31981 caccgataat agccacttta tgatgttgca tattaacact ccctcaatga tctatttcaa
    32041 gttattgttt ttgactataa atcgtaataa ttacgattta taaagtatag acctatcctt
    32101 gagaaatttc aatattaaaa gaaaaacttc cgttattgta gagaattcat ctacatttcg
    32161 gaagttggta tgtgtctttt ttattaaatt taaagatgct acggttagtt caaatgatta
    32221 tgaatgacat ttcatggtat catccagcct cagtacttgg agataacatt ttggcggtga
    32281 gacagaaatc atatttttac aaaatttatt ggggtgtaac ttctccacgt tgtatttcac
    32341 tatccaaatt ttgtttcata ttttcaaaca actcttgaaa tctaacctca tcttgatcag
    32401 aataaccttt aaaaataact gaatctatat tttcctcgtt aaaataataa atttcatctt
    32461 ctacaacacc aatcggatat ttacatgcag aatagtcaaa catatacttt tgattttcaa
    32521 tttctacaat tggtcctcta ttaataatca ttaacttttg cgaaccttct tttaaataaa
    32581 taatgcttcc tattgtttcc atttattttc ctcctttgtc accgtctcaa tttcaacatt
    32641 ccttcgcatt tccaagtcaa tcatctcttc ttctgcttta cgaatgccat gttgaatttg
    32701 ctgttgctgc tcgtataacg cttccgttga acgtcgtttc gcaaaatgaa attcatcgcg
    32761 gaactgttcc atacgactag ccatattgct actataataa cgctcaccta aatgttgata
    32821 taattgatct atcgaatgat taagcgcatc actttcatct atcaatgctt taacctctcg
    32881 cttttcatca tataagtcat ccatttgctt atgtttcgct gtgattattt tattgatttc
    32941 atccagttta ttcgacatca tttcaccacg ttttaatttt taccaatttc attaccaatc
    33001 attacgtcag tttggtcaaa agcctcacca actttttgta tattatcgct cgcagttcgt
    33061 attgcttcag caacagattg attcttactc atcaaatcat ctatcacact atgagcatta
    33121 ttatttccac ttactgttgt actttcatct ttatccggtt tcgacacatt attacatgtt
    33181 tcaacagcgc ttttaataga tgatgatgca ctattggtct taaaaatatc actttgaact
    33241 gtactcatca tttacacctc tctctccatt ttaattattt taaaattatt agaaactgta
    33301 tcaatatatg atattcaaac attcaattat acttaaatat tgttttatga ttatattc
//
LOCUS       DAMZKC010000009        33301 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.009, whole genome shotgun sequence.
ACCESSION   DAMZKC010000009 DAMZKC010000000
VERSION     DAMZKC010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 33301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33301
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.009"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(27..1154)
                     /locus_tag="POB55_000313"
     CDS             complement(27..1154)
                     /locus_tag="POB55_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HDI6499309.1"
                     /translation="MKSKYEPLFDKVELPNGVELRNRFVLAPLTHISSNDDGTISDVE
                     LPYIEKRSQDVGITINAASNVSDVGKAFPGQPSIAHDSDIEGLKRLATAMKKNGAKAL
                     VQIHHGGAQALPELTPGGDVVAPSPISLKSFGQKQEHSAREMTNEEIEQAIKDFGEAT
                     RRAIEAGFDGVEIHGANHYLIHQFVSPYYNRRNDVWANQYKFPVAVIEEVLKAKEAYG
                     NKDFIVGYRLSPEEAESPGITMEITEELVNKISHMPIDYIHVSMMDTHATTREGKYAG
                     QERLPLIHKWINGRMPLIGIGSIFTADEALDAVENVGVDLVAIGRELLLDYQFVEKIK
                     DGREDEIINYFDPEREDNHHLTPNLWHQFNEGFYPLPRKDK"
     gene            complement(1516..1893)
                     /locus_tag="POB55_000314"
     CDS             complement(1516..1893)
                     /locus_tag="POB55_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HDI6499310.1"
                     /translation="MNNYKIGQHIKVRVTGIQPYGAFVETPNHTEGLIHISEIMDDYV
                     HNLKKFLSEGQIVKAKILSIDDEGKLNLSLKDNDYFKNYERKKEKQSVLDEIRETEKY
                     GFQTLKERLPIWIKQSKRAIRND"
     gene            complement(2308..2901)
                     /locus_tag="POB55_000315"
     CDS             complement(2308..2901)
                     /locus_tag="POB55_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDI6499311.1"
                     /translation="MANYPQLNKEVQQGEIKVVMHTNKGDMTFKLFPNIAPKTVENFV
                     THAKNGYYDGITFHRVINDFMIQGGDPTATGMGGESIYGGAFEDEFSLNAFNLYGALS
                     MANSGPNTNGSQFFIVQMKEVPQNMLSQLADGGWPQPIVDAYGEKGGTPWLDQKHTVF
                     GQIIDGETTLEDIANTKVGPQDKPLHDVVIESIDVEE"
     gene            2965..3348
                     /locus_tag="POB55_000316"
     CDS             2965..3348
                     /locus_tag="POB55_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase-associated lipoprotein B"
                     /protein_id="HDI6499312.1"
                     /translation="MKLYRFSHKTGSYGVTIVEENGDDVLVKVEQVIKHPKQGDLHNP
                     TETEGVFFHERKALSHFEKRYTKRSQLRDFNVDVLRYEDSLQQAITDLETKLKAQQTK
                     HAEMSLASLARLKEDYSLQYKENFK"
     gene            3479..5884
                     /gene="mnhA1"
                     /locus_tag="POB55_000317"
     CDS             3479..5884
                     /gene="mnhA1"
                     /locus_tag="POB55_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit A"
                     /protein_id="HDI6499313.1"
                     /translation="MSLLHIAVILPLIFALIIPILYRFFKRIHLGWFVLPVPIVIFIY
                     MLTLIKTTMSGNTVMKTLNWMPHFGMNFDLYLDGLGLLFSLLISGIGSLVVLYSIGYL
                     SKSEQLGNFYCYLLLFMGAMLGVVLSDNVIILYLFWELTSFSSFLLISFWRERQASIY
                     GAQKSLIITVFGGLSLLGGIILLAIPTQSFSIQYMIQHASEIQNSPFFIFAMILIMIG
                     AFTKSAQFPFYIWLPDAMEAPTPVSAYLHSATMVKAGLYLIARMTPIFAASQGWVWTV
                     TLVGLITLFWASLNATKQQDLKGILAFSTVSQLGMIMAMLGIGAISYHYQGDDSKIYA
                     AAFTAAIFHLINHATFKGALFMITGAVDHSTGTRDVKKLGGLLTIMPISFTITVITAL
                     SMAGVPPFNGFLSKESFLETTFTASQANLFSVDTLGYLFPIIGIVGSVFTFVYSIKFI
                     MHIFFGQYKPEQLPKKAHEVSILMLLSPAILATLVIVFGLFPGILTNSIIEPATSSIN
                     HTVIDDVEFHMFHGLTPAFLSTLVIYILGILLIVTFSYWVKLLQRQPGKLTFNYWYNR
                     SANVIPNYSEKMTNSYVTDYSRNNLVIIFGALILLTFVTIFSVPFNINFKDVSPIRIF
                     EVCIVILLLSAAFLILFAKSRLFSIIMLSAVGYAVSVLFIFFKAPDLALTQFVVESIS
                     TALFLLCFYHLPNLNRYNEKRSFQLTNALIAGGVGLSVIIIGLIAYGNRHFESISKFY
                     QEHVYDLAHGKNMVNVILVDFRGMDTLFESSVLGIAGLAVYTMIKLRKKRQTQGNEVK
                     NHE"
     gene            5877..6305
                     /gene="mnhB1"
                     /locus_tag="POB55_000318"
     CDS             5877..6305
                     /gene="mnhB1"
                     /locus_tag="POB55_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit B"
                     /protein_id="HDI6499314.1"
                     /translation="MNRQQNDLILQFAAVIIFFMVMVFGFSLFLAGHYTPGGGFVGGL
                     LFASSLVIITIAFDIETMRKIFPLDFKILIGIGLVFCIATPIASWFLGKNFFTHVTFD
                     IPLFILEPVHMTTAVFFDFGVLCAVVGTVMTIIISIGENE"
     gene            6305..6646
                     /gene="mnhC1"
                     /locus_tag="POB55_000319"
     CDS             6305..6646
                     /gene="mnhC1"
                     /locus_tag="POB55_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit C"
                     /protein_id="HDI6499315.1"
                     /translation="MEIIMIFVSGILTAISVYLVLSKSLIRIVMGTTLLTHAANLFLI
                     TMGGLKHGTVPIYEANVKSYVDPIPQALILTAIVIAFATTAFFLVLAFRTYKELGTDN
                     VESMKGVPEDD"
     gene            6639..8135
                     /gene="mnhD1"
                     /locus_tag="POB55_000320"
     CDS             6639..8135
                     /gene="mnhD1"
                     /locus_tag="POB55_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit D"
                     /protein_id="HDI6499316.1"
                     /translation="MIESNMLVLTLVIPVITAILLVFIGKRPIIKRYVALGGTLLTLV
                     AAIINLANVVKHGPIRVELGSWKAPYSIVFVLDIFSALLIITSIIITAIVILYSYQTI
                     GIERERYYYYFSVLFMLIGIIGAFTTGDIFNLFVFFEVFLMSSYFLLVIGSTKIQLQE
                     TIKYVLVNVVSSSFFVMGVAILYSVVGTLNLADISNKLANLSAHDSGLVNIVFILFIF
                     VFATKAGVFPMFVWLPSAYYAPPIPIIAFFGALLTKVGVYAIARTLSLFFSDNVSFSH
                     YVILFLALLTIIFGCVGAVAYANIKKIILYNVMIAVGVILVGVAMMTESGMIGAIYYT
                     LHDMLVKLALFLLIGIMIKITGTADLRQFGGLIKRYPVLGWSFFIAALSLAGIPPLSG
                     FYGKFFIVQSTFERGFYLSGVIVLLSSLVVLYSVIRIFLQGFFGQPKGYDLNNKVDVK
                     YLTTIAIVAVVITVLYGLSADYLYPMVKAGAETFYNPSTYVKAVLGGK"
     gene            8137..8616
                     /gene="mnhE1"
                     /locus_tag="POB55_000321"
     CDS             8137..8616
                     /gene="mnhE1"
                     /locus_tag="POB55_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit E"
                     /protein_id="HDI6499317.1"
                     /translation="MAVQLVLNFIIAVFWLFVTNSYTTNNFVLGFIFGLVLVYLLHRV
                     LPGRFYVITLYRIIKLVIIFLIELIKANFDVLKIIIKPSIKNEPGFFVYHTDLKKDWQ
                     IVLLSNLITLTPGTVVLGVSDDRTKIYIHAIDFSTKEQEVESIKTSLEKIVREVGEI"
     gene            8616..8909
                     /gene="mnhF1"
                     /locus_tag="POB55_000322"
     CDS             8616..8909
                     /gene="mnhF1"
                     /locus_tag="POB55_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit F"
                     /protein_id="HDI6499318.1"
                     /translation="MNHNVIIVIALIIVVISMLAMLIRVVLGPSLADRVVALDAIGLQ
                     LMAVIALFSILLNIKYMIVVIMMIGILAFLGTAVFSKFMDKGKVIEHDQNHTD"
     gene            8887..9243
                     /gene="mnhG1"
                     /locus_tag="POB55_000323"
     CDS             8887..9243
                     /gene="mnhG1"
                     /locus_tag="POB55_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit G"
                     /protein_id="HDI6499319.1"
                     /translation="MIKIILISLALIFVIIGALISALAAIGLLRLEDVYSRAHAAGKA
                     STLGAMSLLFGTFLYFIATQGFVNMQLIVAIIFVLITGPLSSHMIMKAAYNIKTPYTK
                     KTKVDEISEDLKDTKL"
     gene            9492..10646
                     /locus_tag="POB55_000324"
     CDS             9492..10646
                     /locus_tag="POB55_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HDI6499320.1"
                     /translation="MHWTIIGGGIQGTAIAQKLLSSGLTTDRLTIIDPHETFCQRFNS
                     FTNRIEMPYLRSPIVHHVHPQPFHLKQFAKQHQYTNAFYGPYQRPELTMFMDHIAHAS
                     KQYQLEDCLVQGLVQTLDKQEDKWHIKLEDGQIITTDCVVIAIGSTNIPFMPDILKDK
                     QNVNHIFEKEHDQVVYDKTDHIVGSGITAAHLALKLLNHDNDKKIHLWLNKDIEIHDF
                     DADPGWLGPKNMSTFLSTKSMPERNAIVQRERHKGSMPHELYLRLKKHIKNGRINVHK
                     TPITQISDGLINTENNSVPYQQIMVATGFEQDFMSQPLIKQLIQNYDAPINECNYPVI
                     SEKLEWIPNLFVAGCFADLELGPFGRNVMGGRKAAERIEQAFLKLQQYSA"
     gene            complement(10702..11076)
                     /locus_tag="POB55_000325"
     CDS             complement(10702..11076)
                     /locus_tag="POB55_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HDI6499321.1"
                     /translation="MTHLLETFEMSIDHQEDGLVVISMPVTDKVKQPFGYLHGGASIA
                     LGETACSLGSANLIDTTKFIPLGLEMNANHIHSAKDGHVTATAEIIHQGKSTHVWDIK
                     IKNDKEQLITVMRGTVAIKPLK"
     gene            complement(11095..12411)
                     /locus_tag="POB55_000326"
     CDS             complement(11095..12411)
                     /locus_tag="POB55_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HDI6499322.1"
                     /translation="MINAVVIAVILMIVLCLCRLNVVISLFISALVGGLISGMSIEKV
                     INVFGKNIVDGAEVALSYALLGGFAALISYSGITDYLVGKIINAIHAENSRWSRVKVK
                     VTIIIALLAMSIMSQNLIPVHIAFIPIVIPPLLSLFNDLKIDRRLIGLIIGFGLCFPY
                     VLLPYGFGQIFQQIIQSGFAKANHPIEFNMIWKAMLIPSMGYIVGLLIGLYVYRKPRE
                     YETRKISDSDNVTELKPYILIVTIVAILATFLVQTFTDSMIFGALAGVLVFFISRAYN
                     WYELDAKFVEGIKIMAYIGVVILTANGFAGVMNATGDIDELVKTLTSITGDNKLFSII
                     MMYVIGLIVTLGIGSSFATIPIIASLFIPFGASIGLDTMALIALIGTASALGDSGSPA
                     SDSTLGPTAGLNVDGQHDHIRDTCVPNFLFYNIPLMIFGTIAAMVL"
     gene            complement(12822..14297)
                     /locus_tag="POB55_000327"
     CDS             complement(12822..14297)
                     /locus_tag="POB55_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family metallopeptidase"
                     /protein_id="HDI6499323.1"
                     /translation="MNFKLNNTLSNEINTLIIGIPEHLNQLERISFNHIDITESLERL
                     KHQHIIGSKVGKIYTTAFDVQDQTYRLITVGLGNLKTRSYQDMLKIWGHLFQYIKSEH
                     IEDTYLLMDSFISKYDQLSDVLMACGIQSERATYEFDHYKSSKKAPFKTNLNLISESL
                     IELDFIHEGISIGQSINLARDFSNMPPNVLTPQTFAEDIVNHFKNTKVKVDVKDYDTL
                     VSEGFGLLQAVGKGSKHKPRLVTITYNGKDKDEAPIALVGKGITYDSGGYSIKTKNGM
                     ATMKFDMCGAANVVGIIEAASRLQLPVNIVGVLACAENMINEASMKPDDVFTALSGET
                     VEVMNTDAEGRLVLADAVFYANQYQPSVIMDFATLTGAAIVALGDDKAAAFESNSKVI
                     LNDILQISSEVDEMVFELPITATERASIKHSDIADLVNHTNGQGKALFAASFVTHFSG
                     QTPHIHFDIAGPATTNKASYNGPKGPTGFMIPTIVQWLKQQ"
     gene            complement(14428..15636)
                     /locus_tag="POB55_000328"
     CDS             complement(14428..15636)
                     /locus_tag="POB55_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDI6499324.1"
                     /translation="MAQDRKKVLVLGAGYAGLQTVTKLQKAISTEEAEITLINKNEYH
                     YEATWLHEASAGTLNYEDVLYPVESVLKKDKVNFVQAEVTKIDRDAKKVETNQGIYDF
                     DILVVALGFVSETFGIEGMKDHAFQIENVITARELSRHIEDKFANYAASKEKDDNDLS
                     ILVGGAGFTGVEFLGELTDRIPELCSKYGVDQNKVKITCVEAAPKMLPMFSEELVNHA
                     VSYLEDRGVEFKIATPIVACNEKGFVVEVDGEKQQLNAGTSVWAAGVRGSKLMEESFE
                     GVKRGRIVTKQDLTINGYDNIFVIGDCSAFIPAGEERPLPTTAQIAMQQGESVAKNIK
                     RILNGESTEEFEYVDRGTVCSLGSHDGVGMVFDKPIAGKKAAFMKKVIDTRAVFKIGG
                     IGLAFKKGKF"
     gene            complement(16090..16449)
                     /locus_tag="POB55_000329"
     CDS             complement(16090..16449)
                     /locus_tag="POB55_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HDI6499325.1"
                     /translation="MPTVILTEAAAYEVKDMLKANEMPDGYLKIKVNGGGCTGLTYGM
                     GAEEAPGENDEVLEYFGLKVLVDKKDAPVLNGTTIDFKQSLMGGGFQIDNPNAIASCG
                     CGSSFRTAKVAGNPENC"
     gene            complement(16462..16698)
                     /locus_tag="POB55_000330"
     CDS             complement(16462..16698)
                     /locus_tag="POB55_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YuzB family protein"
                     /protein_id="HDI6499326.1"
                     /translation="MNPIVEFCLSNMAKGGDYVFNQLENDPDVDVLEYGCLTHCGICS
                     AGLYALVNGDIVEGDSPEELLQNIYAHIKETWIF"
     gene            17015..18079
                     /locus_tag="POB55_000331"
     CDS             17015..18079
                     /locus_tag="POB55_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDI6499327.1"
                     /translation="MKNLVLLGGGYGNMRIMSRILTTSLPQDYTVTLVDRMPFHGLKP
                     EFYALAAGTKSDKDVRMKFPNHPQVNTVYGEINDIDLDAQIVSVGNSKIDYDELIIGL
                     GCEDKYHNVPGAEEYTHSIQTLSKARDTFHSISELPEGAKVGIVGAGLSGIELASELR
                     ESRSDLEIYLYDRGPRILRNFPEKLSKYVAKWFAKNNVTVVPNSNINKVEPGKIYNCD
                     EPKDIDLVVWTAGIQPVEVVRNLPIDINSNGRVIVNQYHQVPTYRNVYVVGDCADLPH
                     APSAQLAEVQGDQIADVLKKQWLNEPLPDKMPELKVQGIVGSLGDKQGFAYIMDRTVT
                     GRLASILKSGVLWLYKYHNG"
     gene            complement(18138..18461)
                     /locus_tag="POB55_000332"
     CDS             complement(18138..18461)
                     /locus_tag="POB55_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YuzD family protein"
                     /protein_id="HDI6499328.1"
                     /translation="MEHVSVVVYGADVICASCVNAPTSKDIYDWLQPLLKRKYPNISF
                     KYTYIDITKDNDNLTDHDLQFIERIEQDELFYPLITMNDEYVADGYIQTKQITRFIDQ
                     KLVNE"
     gene            18562..18804
                     /locus_tag="POB55_000333"
     CDS             18562..18804
                     /locus_tag="POB55_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HDI6499329.1"
                     /translation="MPTEDTTMFDQVAEVIERLRPFLLRDGGDCSLIDVEDGIVKLQL
                     HGACGTCPSSTITLKAGIERALHEEVPGVIEVEQVF"
     gene            complement(19067..20242)
                     /gene="dltD"
                     /locus_tag="POB55_000334"
     CDS             complement(19067..20242)
                     /gene="dltD"
                     /locus_tag="POB55_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HDI6499330.1"
                     /translation="MKLKPFLPILISGAVFIVFLLLPASWFTGLVNEKTVEDNRTSLT
                     DQVLKGTLIQDKLYESNKYYPIYGSSELGKDDPFNPAIALNKHNANKKAFLLGAGGST
                     DLINAVELASQYDKLKGKKLTFIISPQWFTNHGLTNQNFDARMSQTQINQMFQQKNMS
                     TELKRRYAQRLLQFPHVHNKEYLKSYAKNPKETKDSYISGFKENQLIKIEAIKSLFAM
                     DKSPLEHVKPATKPDASWDEMKQKAVEIGKADTTSNKFGIRDQYWKLIQESKRKVRRD
                     YEFNVNSPEFQDLELLVKTMRAAGADVQYVSIPSNGVWYDHIGIDKERRQAVYKKIHS
                     TVVDNGGKIYDMTDKDYEKYVISDAVHIGWKGWVYMDEQIAKHMKGEPQPEVDKPKN"
     gene            complement(20239..20475)
                     /gene="dltC"
                     /locus_tag="POB55_000335"
     CDS             complement(20239..20475)
                     /gene="dltC"
                     /locus_tag="POB55_000335"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002433359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     2"
                     /protein_id="HDI6499331.1"
                     /translation="MEFREQVLNLLAEVAENDIVKENPDVEIFEEGIIDSFQTVGLLL
                     EIQNKLDIEVSIMDFDRDEWATPNKIVEALEELR"
     gene            complement(20493..21707)
                     /gene="dltB"
                     /locus_tag="POB55_000336"
     CDS             complement(20493..21707)
                     /gene="dltB"
                     /locus_tag="POB55_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PG:teichoic acid D-alanyltransferase DltB"
                     /protein_id="HDI6499332.1"
                     /translation="MIPYGDFTFFLIALIALLPVIILGFLGKRSYIYNGVVTAFMIVL
                     IFSSDKHNLFDQKYLSVQLISFIIYVVWQVLLIMFYYHSKPKNNSFSKFVTVMVLSIL
                     PLALVKVLQSTWLGGHQIHFHESKLIEFVGFLGISYVTFKSVQLIMEIRDGSIKEIKV
                     WKLIQFISFFPTISSGPIDRYKRFVKDDKKVPTGNEYRELVLKAIHMIMLGFLYKYIV
                     AYFINTYAIMPLQLDLHGFVNLWLYMYAYSLYLFFDFAGYSLFAIAFSYLFGIKTPPN
                     FDKPFKAKNIKDFWNRWHMTLSFWFRDCIYMRSLFYMSRKKLLKSQFAMSNVAFLINF
                     FIMGIWHGIEVYYIVYGLYHAALFIGYGYYERWRKKHPPRWQNGFTTALSIVITFHFV
                     TFGFLIFSGKLI"
     gene            complement(21704..23161)
                     /gene="dltA"
                     /locus_tag="POB55_000337"
     CDS             complement(21704..23161)
                     /gene="dltA"
                     /locus_tag="POB55_000337"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HDI6499333.1"
                     /translation="MTDIINKLQAFADANPQSIAVRHTTDELTYQQLMDESSKLAHRL
                     QGSKKPMILFGHMSPYMIVGMIGAIKAGCGYVPVDTSIPEDRIKMIINKVQPEFVFNT
                     TDESFESLEGEVFTIEDIKTSQDPVIFDSQIKDNDTVYTIFTSGSTGEPKGVQIEYAS
                     LVQFTEWMLELNKSGNEQQWLNQAPFSFDLSVMAIYPCLASGGTLNLVDKNMINKPKL
                     LNEMLTATPINIWVSTPSFMEMCLLLPTLNEEQYGSLNEFFFCGEILPHRAAKALVNR
                     FPSATIYNTYGPTEATVAVTSIQITQEILDQYPTLPVGVERPGARLSTTDEGELVIEG
                     QSVSLGYLKNDQKTAEVFNFDDGIRTYHTGDKAKFENGQWFIQGRIDFQIKLNGYRME
                     LEEIETQLRQSEFVKEAIVVPVYKNDKVIHLIGAIVPTTEVTDNAEMTKNIKNDLKSR
                     LPEYMIPRKFEWMEQLPLTSNGKIDRKKIAEVING"
     gene            complement(23177..23329)
                     /locus_tag="POB55_000338"
     CDS             complement(23177..23329)
                     /locus_tag="POB55_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HDI6499334.1"
                     /translation="MKSKSKQPPNKYVEAFKPYLLTLLYLAIFITLYLIYGSGDTHNN
                     FIYNEF"
     gene            complement(23377..23487)
                     /locus_tag="POB55_000339"
     CDS             complement(23377..23487)
                     /locus_tag="POB55_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499335.1"
                     /translation="MMTTNYYVESIKLKLNFIMNIDIMNCKKQILKRILY"
     gene            complement(23741..24700)
                     /locus_tag="POB55_000340"
     CDS             complement(23741..24700)
                     /locus_tag="POB55_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HDI6499336.1"
                     /translation="MVKIVVSRKIPDKFYQQLSKLGDVVMWQKSLVPMPKDQFVTALR
                     DADACFITLSEQIDAEILAQSPNLKVIANMAVGYDNIDVESATANNVVVTNTPNVLTE
                     TTAELGFTLMLAIARRIVEAEKYVEADAWQSWGPYLLSGKDVFNSTIGIYGMGDIGKA
                     FARRLQGFNTNILYHNQSRHKDAEADFNATYVSFETLLAESDFIICTAPLTKETHHKF
                     NAEAFEQMKNDAIFINIGRGQIVDETALIDALDNKEILACGLDVLANEPIDHTHPLMG
                     RDNVLITPHIGSASVTTRDNMIQLCINNIEAVMTNQVPHTPVN"
     gene            complement(24733..25512)
                     /locus_tag="POB55_000341"
     CDS             complement(24733..25512)
                     /locus_tag="POB55_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HDI6499337.1"
                     /translation="MKQYKAYLIDLDGTMYMGTDEIDGAKQFIDYLNVKGIPHLYVTN
                     NSTKTPEQVTEKLREMHIDAKPEEVVTSALATADYISEQSPGASVYMLGGSGLNTALT
                     EAGLVIKNDEHVDYVVIGLDEQVTYEKLAIATLGVRNGATFISTNPDVSIPKERGLLP
                     GNGAITSVVSVSTGVSPQFIGKPEPIIMVKALEILGLDKSEVAMVGDLYDTDIMSGIN
                     VGMDTIHVQTGVSTLEDVQNKNVPPTYSFKDLNEAIAELEK"
     gene            complement(25512..25946)
                     /locus_tag="POB55_000342"
     CDS             complement(25512..25946)
                     /locus_tag="POB55_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HDI6499338.1"
                     /translation="MYFVDKDKLTQKLAYLQALTDDYHESKHNHYAFERIAQMLIESS
                     VDIGNMIIDAFILRDPGNYKDVIDILELENVITKETQQAINKTVGIRKQFTYDYTALD
                     IKIIMPMFDDALPYYKQFITEVTTFLHQENVPVTAFGKGENQ"
     gene            26044..26310
                     /locus_tag="POB55_000343"
     CDS             26044..26310
                     /locus_tag="POB55_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3055 domain-containing protein"
                     /protein_id="HDI6499339.1"
                     /translation="MIDMYLYDDNEESQVQFVGFVGEHSRYDLMLVHTNRHYGKTLVL
                     NMQTNKFGIIGTDDLKEEGYIAHILGVNAEEGDEITEYLNEVIH"
     gene            complement(26389..26772)
                     /locus_tag="POB55_000344"
     CDS             complement(26389..26772)
                     /locus_tag="POB55_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HDI6499340.1"
                     /translation="MIKVDQHYFELIENYRECFNEEQFIARYSDILDKYDYIVGDYGY
                     DQLRLKGFYKDSNKKAEMSKRFSNIQDYIFEYCNFGCPYFVLRHLSKQEVKKLIEEVH
                     PSDVIDDDNKLQDVKIKPTIQDTEH"
     gene            complement(26902..27819)
                     /gene="lipA"
                     /locus_tag="POB55_000345"
     CDS             complement(26902..27819)
                     /gene="lipA"
                     /locus_tag="POB55_000345"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HDI6499341.1"
                     /translation="MATKNEEILRKPDWLKIKLNTNENYTGLKKMMREKNLNTVCEEA
                     KCPNIHECWGARRTATFMILGAVCTRACRFCAVKTGLPNELDLNEPERVAESVELMNL
                     KHVVITAVARDDLRDAGSNVYAETVRKVRERNPFTTIEILPSDMGGDYDALETLMASR
                     PDILNHNIETVRRLTPRVRARATYDRTLEFLRRSKELQPDIPTKSSIMVGLGETIEEI
                     YETMDDLRANDVDILTIGQYLQPSRKHLKVQKYYTPLEFGKLRKVAMDKGFKHCQAGP
                     LVRSSYHADEQVNEAAKEKQRQGEAQLNS"
     gene            complement(27903..29222)
                     /locus_tag="POB55_000346"
     CDS             complement(27903..29222)
                     /locus_tag="POB55_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HDI6499342.1"
                     /translation="MRLTIYHTNDIHSHLHEYERIKAYMAEHRPRLNHPSLYVDLGDH
                     VDLSAPITEATLGKKNVALLNEAKCDVATIGNNEGMTISHEALNHLYDEAKFIVTCSN
                     VIDESGHLPNNIVSSYIKDIDGVKILFVAATAPFTPFYRALNWIVTDPLESIKEEIEL
                     QRGKFDVLIVLSHCGIFFDETLCQELPEIDVIFGSHTHHYLEHGEINNGVLMAAAGKY
                     GNYLGEVNLTFEAHKVVHKTAKIIPLKTLPEVETSFEEEGKTLMSNSVIQHPVVLKRS
                     MNHITEAAYLLAQSVCEYTHAQCAIINAGLLVKDIVKDEVTEYDIHQMLPHPINMVRV
                     RLFGVKLKEIIAKSNKQEYMYEHAQGLGFRGNIFGGYILYNLGYIHSTGRYYLNGEEI
                     EDDKEYVLGTIDMYTFGRYFPTLKELPKEYLMPEFLRDIFKEKLLEY"
     gene            complement(29249..30076)
                     /locus_tag="POB55_000347"
     CDS             complement(29249..30076)
                     /locus_tag="POB55_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDI6499343.1"
                     /translation="MLLTITLLVLIGGLSAIIGSIVGIGGGIIIVPTMVYLGVEHGLL
                     HNITTQVAIGTSSVILIVTGLSSSLGYLKTKQVDIKNGSIFLFGLLPGSLLGSFISRY
                     LTFESFNLYFGIFLIFVAILLMVRNKIKPFKIFDKPKYEKTYVDAKGKTYHYSVPPLF
                     AFITTFLIGILTGLFGIGGGALMTPLMLIVFRFPPHVAVGTSMMMIFFSSVMSSIGHI
                     AQGHVAWGYAIILIISSYFGAKIGVKVNQSIKSDTVVTLLRTVMLLMGIYLIIRALI"
     gene            complement(30089..30937)
                     /locus_tag="POB55_000348"
     CDS             complement(30089..30937)
                     /locus_tag="POB55_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HDI6499344.1"
                     /translation="MINIGLTGWGDHYSLYEDLERQTDKLKTYAGHFPVVELDATYYA
                     IQPERNILKWIKETPDTFEFVVKIHQALTLHADYKTFADTRQELFDQFKNMLEPLHTQ
                     KKLAMVLVQFPPWFDCNAQNIKYILYVRQQLQAFPMCVEFRHQSWFSDAFKEQTLAFL
                     TEHQIIHAVVDEPQVKDGSVPLVNRITNEIAFVRYHGRNHYGWTKKDMSDQEWRDVRY
                     LYDYNEQELIDLAQKAQILAQKAKKVYVIFNNNSGGHAANNAKTYQRLLNIEYEGLAP
                     QQLKLF"
     gene            complement(31113..32180)
                     /locus_tag="POB55_000349"
     CDS             complement(31113..32180)
                     /locus_tag="POB55_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HDI6499345.1"
                     /translation="MWNKNRLTQMLSIEYPIIQAGMAGSTTPKLVASVSNSGGLGTIG
                     AGYFNTQQLEDEIDYVRQLTSNSFGVNVFVPSQQSYTSSQIENMNAWLKPYRRALHLE
                     EPVVKITEEQQFKCHIDTIIKKQVPVCCFTFGIPSESIIKRLKEANIKLIGTATSVDE
                     AIANEKAGMDAIVAQGSEAGGHRGSFLKPKNQLPMVGTISLVPQIVDVVSIPVIAAGG
                     IMDGRGVLASIVLGAEGVQMGTAFLTSQDSNASELLRDAIINSKETDTVVTKAFSGKL
                     ARGINNRFIEEMSQYEGDIPDYPIQNELTSSIRKAAANIGDKELTHMWSGQSPRLATT
                     HPANTIVSNIINQINQIMQYK"
     gene            complement(32194..33234)
                     /locus_tag="POB55_000350"
     CDS             complement(32194..33234)
                     /locus_tag="POB55_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDI6499346.1"
                     /translation="MIIAIIILIFISFFFSGSETALTAANKTKFKTEADKGDKKAKGI
                     VKLLEKPSEFITTILIGNNVANILLPTLVTIMALRWGISVGIASAVLTVVIILISEVI
                     PKSVAATFPDKITRLVYPIINICVIVFRPITLLLNKLTDSINRSLSKGQPQEHQFSKE
                     EFKTMLAIAGHEGALNEIETSRLEGVINFENLKVKDVDTTPRINVTAFASNATYEEVY
                     ETVMNKPYTRYPVYEGDIDNIIGVFHSKYLLAWSNKKENQITNYSAKPLFVNEHNKAE
                     WVLRKMTISRKHLAIVLDEFGGTEAIVSHEDLIEELLGMEIEDEMDKKEKEKLSQQQI
                     QFQQRKNRNVSI"
BASE COUNT        10492 a         5970 c         4986 g        11853 t
ORIGIN      
        1 tatcattagt ggtaacagtt aaacacttat ttatctttac gtggtaatgg atagaatcct
       61 tcattaaatt gatgccataa attaggagtt aagtgatgat tatcttctct ctctggatca
      121 aagtaattaa taatttcatc ttcccgtcca tctttaattt tttcaacaaa ttgataatcc
      181 agtagtagct ctctaccaat ggctactaag tcaacaccaa cattttcaac tgcatctaaa
      241 gcttcgtcag ctgtgaaaat tgaaccaata ccgataagtg gcatacgacc atttatccat
      301 ttgtgaatta aaggcagtct ttcttgtcca gcgtatttac cttcacgtgt cgttgcatgc
      361 gtatccatca tcgaaacatg aatatagtcg attggcatat ggctaatttt attaacgagt
      421 tcctctgtaa tttccattgt gattcctgga gactccgctt cctctggaga taatctgtat
      481 ccaactataa agtctttatt gccatacgct tctttcgctt taagcacctc ttcaatcaca
      541 gcgaccggga atttatattg atttgcccaa acatcatttc ttctattata gtatggtgat
      601 acaaattgat gaattaagta atgattcgcg ccatgtattt caacaccatc aaatcctgct
      661 tcaattgcac gtcgcgttgc ttcaccaaaa tccttgattg cttgttcaat ctcttcattc
      721 gtcatttctc tagcactatg ttcttgtttc tgaccgaaac tttttaaaga aattggactt
      781 ggtgctacga cgtctccacc aggtgttaat tcaggcaatg cttgtgcacc gccatgatgt
      841 atttgtacga gtgctttggc accgtttttc ttcattgctg tagctaatcg ttttagtcct
      901 tcaatatcac tgtcatgcgc gattgatggc tgtcctggaa atgcttttcc gacatcactc
      961 acattactcg cagcattaat tgtaatacca acatcttgtg aacgcttttc aatataagga
     1021 agttctacat ctgaaatagt accatcatca tttgaagaaa tatgtgttaa aggggctaac
     1081 acaaatcgat ttctcaactc tactccattt ggtaattcta ctttatcaaa caatggttcg
     1141 tatttacttt tcattactta attcctcctt aaaattattg agttgactat aacatgtaat
     1201 tttttcattc tccaattatc tgtttatata gttgcaattc gtttaatact ttaagttgta
     1261 gttctcattt ctaaaataaa ttaaaaaaac aaatccacat acaatattgc aaatcaatga
     1321 ttctatctat caaagataat ggatatgtca ccttaaacca tttgtacgtg taaatagaat
     1381 caatcatatt acgttatcgt agacaaaaag agattagtat ctaataaaat ttaatagata
     1441 ctaatctctt ttatagtcta agaaactttc atttcagacc ctttgtatga ttttcggtac
     1501 gatttatctg ttcctttagt cgtttcgaat tgctcgcttt gactgtttta tccagattgg
     1561 taagcgttct ttaagtgttt gaaacccata tttttctgtt tctctgattt catctaatac
     1621 tgattgtttt tccttcttac gctcataatt tttgaagtaa tcattatcct ttaatgatag
     1681 attaagcttt ccttcatcat ctatagacaa aattttagct ttaacaattt ggccttctga
     1741 tagaaatttc ttcaaattat gaacgtagtc atccataatt tctgatatat gaatcagtcc
     1801 ttcagtatga ttaggggtct caacaaacgc accgtatggt tgaataccag ttacacgcac
     1861 cttgatatgt tggccaattt tgtagttatt caacttaata accccttatt tcgtttactt
     1921 aacactatta tcatagcaca aatcgaaatg gtggactaat taaaacatca aatcatacga
     1981 atattatttt ataaatgaat aagaattcta caaacttgga tttacaaata aaattagcaa
     2041 caaactccta tctaaatcaa tatgatatcc agctactttg ttttcaattt attaactatt
     2101 tgaatagcaa ttctctttaa aaacataaaa agcaacctca actattatct ttagaataca
     2161 catctattta aaaactgtgc tttcttgata aatatattga gattgctcat catgttagtt
     2221 tatttgtttc gttggaatat atactaatcc attcataaat tagctttatc cgagtttaaa
     2281 atgttggtag ttaattatgt ttagctatta ttcttcaaca tcaatagatt caattacaac
     2341 atcatgaagt ggtttgtctt gtggtcccac ttttgtattt gcaatatctt ctaaagtagt
     2401 ttcaccatca atgatttgac cgaatactgt atgtttttga tctaaccatg gtgtaccacc
     2461 cttttcgcca tatgcatcaa cgattggttg aggccagcca ccatctgcaa gttgacttaa
     2521 catattttga ggtacttctt tcatttgaac aatgaaaaat tgtgaaccat tagtattagg
     2581 tcctgagtta gccattgata atgcgccata taagttaaat gcatttaatg aaaattcatc
     2641 ttcaaaagca ccgccataaa tactttcgcc acccatacca gtagctgttg gatcgccacc
     2701 ttgaatcatg aagtcattaa tgacacggtg gaatgttatt ccatcataat aaccattttt
     2761 tgcatgtgtc acaaaatttt caactgtttt tggtgcaata tttggaaata atttgaatgt
     2821 catgtcacct ttatttgtgt gcataaccac tttaatttca ccttgttgta cttctttgtt
     2881 taactgtgga tagttagcca tttatattct ccattcatgt tatgatacat atatattaac
     2941 atacttttaa gggaggaaaa tagcatgaag ctatatcgat tttcacacaa aacaggtagc
     3001 tatggtgtca cgattgttga agaaaatgga gacgatgtcc ttgttaaagt tgaacaagtt
     3061 atcaaacacc cgaaacaagg tgatttacat aaccctactg aaactgaagg tgtatttttc
     3121 catgaacgca aagcattaag ccattttgaa aaacgctata cgaaacgttc acaattacgc
     3181 gacttcaatg ttgatgtatt gcgctatgaa gattcacttc aacaagcaat tacagattta
     3241 gaaactaaac ttaaagctca gcaaacaaaa catgctgaaa tgtctttagc tagtttagca
     3301 cgcttaaaag aagattattc acttcaatac aaagaaaatt ttaaataaaa aatgacgtgt
     3361 ttcttcttat ttaaatactt ctgaaaataa agcacaacaa ggagtgtgta cgtttttcag
     3421 aactatatca ttcatgtata atgaataaca taaatatttt ttaaaggagg acaacatagt
     3481 gagtttgtta catattgcgg tgatattacc gttaatattc gcactcatta ttcctatcct
     3541 gtatagattt tttaaacgta tacatttagg atggttcgta ttacctgtcc ctatcgtcat
     3601 atttatttat atgcttacat taattaaaac gacgatgtca ggtaatactg taatgaaaac
     3661 cttaaattgg atgcctcatt ttggtatgaa ctttgattta tatcttgatg gcttaggttt
     3721 actctttagt ttgcttattt caggaatcgg cagtttagta gtattatatt caatcggcta
     3781 cttaagtaag tcagaacaac tagggaattt ttattgttat ttattactat ttatgggtgc
     3841 aatgttaggt gtcgtacttt cagataatgt tattattcta tatttattct gggagctaac
     3901 gtcattttct agttttttac ttatttcatt ctggcgcgaa cgacaagcat ctatatatgg
     3961 tgctcaaaaa tcgctcataa tcacagtatt tggtggtcta tcattattag gtggtatcat
     4021 cttactcgct ataccaacac aatcatttag cattcagtac atgattcaac atgcttctga
     4081 aattcaaaat agcccattct tcatttttgc gatgatttta atcatgattg gtgcttttac
     4141 aaaatctgct cagtttccat tttatatttg gctaccagat gcaatggaag cacctacacc
     4201 agtcagtgct tatcttcatt cagcaacaat ggttaaagca gggttatatt taatcgctcg
     4261 aatgacacca atcttcgcag catcgcaagg ttgggtatgg actgtcacac tcgttggttt
     4321 aattacatta ttctgggctt cgctaaacgc aacaaaacaa caagacttga aaggtatact
     4381 ggcattttca acagtatccc aattaggtat gattatggcg atgcttggaa tcggtgccat
     4441 tagttatcac tatcaaggcg atgatagcaa aatatatgct gctgcattta cggctgcaat
     4501 attccattta atcaaccatg caacatttaa aggtgcccta tttatgatta caggtgcagt
     4561 ggatcattct actggtacac gtgatgttaa gaaattaggt ggcttactaa caattatgcc
     4621 tatttcattt acaatcactg ttattacagc attaagtatg gctggtgtac caccgttcaa
     4681 tggtttctta tcaaaagaat catttttaga aacaacattt acagcaagcc aagcaaattt
     4741 attcagtgtt gatacattag gctacctatt cccaatcatt ggtatagtgg gcagtgtctt
     4801 cacatttgta tattcaatta aatttataat gcatatattt ttcggacaat ataaacctga
     4861 acaattacct aaaaaagcac acgaagtatc aatattaatg cttttatcac cagctatttt
     4921 agctacatta gtaattgtat ttggtttatt ccctggcatt ttaactaatt cgattattga
     4981 accagctaca tcttcgatta atcatacggt aattgatgat gttgaattcc atatgttcca
     5041 tggattaacg cctgcctttt tatcaacatt agtgatttat attttaggta tcttactaat
     5101 tgtgacattt agttattggg tgaaattgtt gcaacgacaa cctggtaaac taacattcaa
     5161 ttattggtac aacagaagtg caaatgttat tccaaactac tctgaaaaga tgacgaatag
     5221 ctatgttacc gattattcac gtaataattt agttatcatt tttggtgcat taattttatt
     5281 aacatttgtc actatattct cagtaccttt caacattaat tttaaagacg ttagtccgat
     5341 tagaattttc gaagtttgta tcgtaatttt attattaagt gctgcctttt taattctttt
     5401 cgctaaatca agattattta gcatcattat gttaagtgca gttggatatg ctgtgtctgt
     5461 attgtttata ttctttaaag cacccgactt agcattgaca cagtttgtcg ttgaatctat
     5521 ttcaacagca ttgttcttat tatgcttcta ccacttacca aatttaaatc gttataatga
     5581 aaagcgctca ttccagctta ctaatgcttt aattgctggt ggtgttggtt tgtccgtcat
     5641 tattatcggt ttaattgcat acggaaatag acattttgaa tcgatatcta agttttatca
     5701 agagcacgtt tatgatttag ctcacggtaa aaacatggta aacgtcatac ttgttgattt
     5761 ccgtggtatg gatactttat tcgagtcatc tgtgcttggt atcgcaggat tagctgtata
     5821 tacaatgatt aaattacgta aaaagcgtca aacgcaaggt aacgaggtga aaaatcatga
     5881 atagacaaca aaatgatcta attttgcaat ttgctgcagt tattatcttt ttcatggtaa
     5941 tggtctttgg tttttcactt ttcttagcag gacattatac acctggtggc ggtttcgtcg
     6001 gcggattatt attcgcaagt tcacttgtta tcattacgat tgcatttgat atagaaacaa
     6061 tgcgtaaaat tttcccatta gattttaaaa tcttgatagg tatcggatta gttttttgta
     6121 ttgcgacgcc tatcgcaagt tggtttttag gcaaaaattt ctttacgcat gtcacatttg
     6181 atataccgtt atttatatta gaacctgttc atatgacaac agcagtattc tttgatttcg
     6241 gtgttttatg tgcagtagtt ggtactgtca tgacaattat tatttcgatt ggagagaacg
     6301 aatagtggaa attattatga tttttgttag tggtattctc acagcaatta gtgtctatct
     6361 cgttttgtct aaaagtctga tacgaattgt tatgggaact acactattaa cacatgcagc
     6421 aaatttattt ttaataacta tgggcggact taaacatggt actgttccaa tttatgaagc
     6481 gaacgtaaaa agctatgttg atcctatccc gcaagcactt attttaacag caatcgttat
     6541 cgcctttgcg acaacagcct ttttcttagt attagcattt agaacatata aagaattagg
     6601 cacagataac gttgagagta tgaaaggagt tccagaagat gattgaaagt aatatgcttg
     6661 tgttaacgct agttattcca gtaattacag ccatattact tgtctttatt ggaaagcgtc
     6721 ctattataaa gcgttatgtc gcactaggcg gtacattatt aaccttggtc gcggcaatta
     6781 tcaatttagc aaatgttgtt aaacatggac caatacgcgt agagctaggt tcatggaaag
     6841 caccatacag tattgtcttt gtattagaca tattcagtgc cctactaata attactagta
     6901 ttattattac agctattgtg attttatatt cataccaaac aattggtatt gaacgtgaaa
     6961 gatattatta ttatttttca gtactattta tgttaatagg tatcattggt gcattcacaa
     7021 ctggtgacat tttcaaccta ttcgtatttt tcgaagtatt tttaatgtca tcttatttct
     7081 tattagtaat tggttctaca aaaattcaac tacaagaaac aataaaatat gttcttgtta
     7141 acgttgtatc gtcatccttc ttcgtaatgg gtgtagcgat tttatattca gtagtaggaa
     7201 cattgaatct agcagatata agcaataaat tagctaacct ttcagcgcat gacagtggct
     7261 tagtcaatat cgttttcata ctatttatct ttgtttttgc tacaaaagct ggcgtattcc
     7321 caatgtttgt ttggttacct agtgcatact atgcaccacc aattccaatc attgcattct
     7381 ttggtgcttt gttaacaaaa gttggtgttt atgcaatcgc tagaacattg agcctattct
     7441 ttagtgataa tgtaagcttt tcacattatg tcattttatt cttagcacta ttaacgatca
     7501 tctttggttg tgtcggtgct gttgcatatg ccaacattaa aaaaatcatc ctatacaatg
     7561 tcatgattgc tgtaggtgtg atcttagtcg gcgttgcaat gatgacagag tcaggtatga
     7621 ttggggctat ttactataca ttgcacgaca tgttagtaaa acttgcatta ttcttgctta
     7681 tcggaattat gattaaaatt acaggcactg ctgatttaag acaatttggt ggcttaatta
     7741 aacgatatcc tgtacttggt tggtcattct ttatcgcagc attaagttta gctggcattc
     7801 ccccactcag tggcttttat ggaaaatttt ttattgtcca atcaacattt gaaagaggat
     7861 tttatttaag tggtgtcatc gtcttattat caagtttagt cgtattatat tctgtcattc
     7921 gcatattcct acaaggcttt ttcggtcaac caaaaggata tgatcttaat aacaaagtcg
     7981 acgtcaaata tttaactacg attgcaatcg ttgcagttgt gataactgtt ttatatggat
     8041 tgtcagcaga ttacttatat ccaatggtaa aagctggtgc agaaacattc tacaatccat
     8101 caacctacgt taaagctgta ttaggaggta aataacatgg ctgtacaatt agtattaaac
     8161 tttattatcg cagtgttttg gttgtttgtg acaaatagtt acacaacaaa taactttgtg
     8221 ttaggtttca tctttggcct agttcttgtt tacctcttac acagagtgtt accaggtaga
     8281 ttttatgtca tcacgctata tcgtattatt aaattagtta ttattttctt aatcgaactg
     8341 attaaagcta attttgatgt tttgaaaatt attattaaac cttcgattaa aaatgaacca
     8401 ggattctttg tttatcacac agatttaaaa aaagattggc aaattgtctt gctgtctaat
     8461 ttaattacat taacacctgg tactgtagtg cttggtgtaa gtgacgatcg tacaaaaatt
     8521 tatattcatg ccatagattt ttcaactaaa gagcaagaag tagaaagtat taaaacatca
     8581 ttagagaaaa ttgtaagaga ggtgggcgaa atataatgaa tcataatgtt attatcgtta
     8641 ttgcattaat catagttgtc atttctatgt tagctatgct cattcgcgtt gtgctaggcc
     8701 catcacttgc cgatcgtgtt gtcgcattag atgcgattgg tcttcaatta atggcagtta
     8761 tagcattatt cagtatttta ttaaatatta aatacatgat tgtcgttatt atgatgattg
     8821 gtatattagc ttttttaggt actgcagtat tctctaaatt tatggacaaa ggtaaggtga
     8881 ttgaacatga tcaaaatcat actgattagt cttgcactta tctttgttat catcggtgct
     8941 ttaattagcg ccctggcagc tataggatta ttgagacttg aagatgtata ttcacgtgca
     9001 catgctgccg gaaaagcatc aacattaggt gcaatgtcat tactatttgg tacgttttta
     9061 tattttattg ctactcaagg ttttgtaaat atgcaattaa tcgttgcgat tatctttgta
     9121 ttaattacag gtcctctttc aagtcatatg attatgaaag cagcatataa tattaaaacg
     9181 ccttatacta aaaagactaa agtcgatgaa atttcagaag acttaaaaga cacaaaattg
     9241 tagattatat aacaaaatca tgaatataaa tcaacaacaa acagcagtaa gatgattcca
     9301 aattaggaat gattttactg ctgttttctt ttgacattgt tacctctttt tcaatggttt
     9361 tttctttgac tacagcttcg ccctatctac atatatctct ttaatttaat tgcctttcat
     9421 gtcgttatgt attatgataa taataattat aaatcgtaac gattacgctt taaaaagaga
     9481 gaggttttat tatgcattgg acaattatcg gcggtggcat acagggaact gcaatcgcac
     9541 aaaaactatt atcaagcgga ttaacaacag accgattaac aatcattgac ccacacgaaa
     9601 ctttttgcca aaggtttaac tcatttacaa atcgaataga aatgccttat ttaagatcac
     9661 cgattgtaca tcacgtacat ccacaaccgt tccatctaaa acaattcgct aaacagcacc
     9721 aatatacaaa tgctttttat ggtccttatc aacgacctga attgacaatg tttatggatc
     9781 atattgcaca tgcttctaaa caatatcaat tagaggattg cttggttcaa ggtttagttc
     9841 aaactttaga taaacaagaa gacaaatggc atatcaagtt agaagatgga caaattatca
     9901 ctacagattg cgtcgttatt gcaataggta gtacaaatat tccgtttatg cctgacattt
     9961 taaaagacaa acagaatgta aatcatatct tcgagaaaga acatgatcaa gtagtatatg
    10021 ataagaccga tcatatcgtt ggtagtggca ttacggctgc acatcttgca cttaaattgt
    10081 taaatcatga taacgataaa aagattcatt tatggctaaa taaagatatt gaaatacatg
    10141 actttgatgc tgatcctggt tggttaggtc cgaagaatat gtctacattt ttaagtacta
    10201 aaagcatgcc tgaaagaaat gccattgtac aacgtgaacg tcataaagga tcaatgcctc
    10261 acgaactgta cttacgcctt aaaaaacata ttaaaaatgg tcgtataaat gtgcataaaa
    10321 cacctatcac tcaaattagt gatggtttaa ttaacactga aaataattct gttccatatc
    10381 aacagattat ggttgcaact ggttttgaac aagattttat gtcacaacca cttattaagc
    10441 aattaataca aaattatgat gcacctatca acgaatgtaa ttaccctgtt atttccgaaa
    10501 aattagaatg gataccaaat ctatttgtcg caggatgctt tgcagactta gaattaggac
    10561 catttggtag aaatgttatg ggtggccgta aagctgccga acgcattgaa caagcatttc
    10621 taaaattaca acaatatagc gcataaaaaa actgctagcc gtagttatga atatctcata
    10681 acatttcagc tagcagttct tttattttaa aggcttaata gcaactgtac cacgcataac
    10741 tgtaattaat tgttctttgt cattcttaat ttttatatcc catacatgtg tcgacttacc
    10801 ttgatgaata atttcagctg tcgcagtaac atgaccatct ttagcagaat gaatatgatt
    10861 ggcattcatc tctaaaccta atggaataaa tttggttgta tcaattagat tagcagatcc
    10921 taatgaacat gctgtttcac ctaaagcaat cgaagcacca ccatgtaaat atccaaatgg
    10981 ttgttttact ttatcagtaa caggcataga aataacaact aaaccatctt cctggtgatc
    11041 tattgacatc tcaaatgtct ctaataaatg agtcatattt tcacctcttt atgcttatag
    11101 taccatagca gcaatagtac cgaaaatcat taaaggaata ttataaaaca agaagtttgg
    11161 tacacatgta tcgcgtatat gatcatgttg gccatcaaca tttaatcccg cagttggtcc
    11221 taatgttgaa tcacttgcag gtgaacctga gtcacctaat gcactcgctg ttccaatcaa
    11281 tgcgattaat gccattgtat ctagtccaat tgacgctcca aaaggaatga ataatgatgc
    11341 gataatagga attgttgcaa atgatgatcc aatacctaaa gtgacaatta aacctatcac
    11401 atacatcatg ataatgctaa ataatttatt atcaccagta atacttgtta aagttttaac
    11461 taattcatct atatcaccag tagcattcat tacaccagca aatccatttg ctgttaaaat
    11521 aactacacca atataagcca taattttaat accttcaaca aacttagcat ctaattcata
    11581 ccaattatat gcacgtgaaa taaagaatac gagtacccct gccagtgcac caaaaatcat
    11641 tgaatctgta aatgtttgta ctaaaaatgt agctagtatt gctacaattg ttactattaa
    11701 gatatatggt tttaactctg taacattgtc actatctgaa attttacgtg tttcatattc
    11761 acgtggttta cgatatacat ataaaccgat aagtaagcca acaatatacc ccattgaagg
    11821 aataagcatt gctttccaaa tcatattaaa ctcaattggg tgattcgcct ttgcaaagcc
    11881 actttgaata atttgctgga aaatttgacc gaatccatat ggtaataaca catacgggaa
    11941 acataaacca aaaccgataa tcaaaccgat taaacgtcta tctattttta agtcattaaa
    12001 cagacttaac aatggtggga tgacaattgg aatgaatgca atatgtacag gaattaagtt
    12061 ttgactcatg atactcatag ctaataatgc aatgattatt gtcactttga ctttaactct
    12121 tgaccatcga ctattttcag cgtgaattgc atttataatt tttcctacta aatagtctgt
    12181 gataccactg tatgaaatta atgctgcaaa tccacctaat aaagcatagc ttaatgctac
    12241 ctcagcacca tcgactatat ttttcccaaa tacatttata actttttcaa tgctcatgcc
    12301 tgaaattaag ccaccaacta gcgcactgat aaataagctt ataactacgt ttaatcgaca
    12361 taaacatagc acaatcatta aaattactgc tattactact gcatttatca tatgacaacc
    12421 atcctttatc actctatcga actaaagcgt ttatagacta catagtatca gtagctttaa
    12481 cacctgtcaa tagcaaacta taccaataat ctttattttt tagttaattg tctttcttaa
    12541 aataatttta gctttcatta aattaaacaa ttttacaagc ttggaacacc aatcaaaatc
    12601 ctaagttcta aaatgcaata ttagtagtcg ttgactgaat gaacatatgc ttataatatt
    12661 tttttgcaat gctagtcaag ttgatttatg ctcacaagga tatgcgattt atattttctt
    12721 acaacaatga aaatgcctga tacaatgcga tcctttatac tttcaagagt taaattagaa
    12781 atgtatatca agacacaagt tgtatcaggc aaataattaa attattgttg ttttaaccat
    12841 tgtactatcg tcggaatcat aaatcctgtt ggcccttttg gaccattata tgaagcttta
    12901 ttagtcgttg ctggacctgc aatatcgaaa tgaatgtgag gtgtttgacc actaaaatgt
    12961 gttacaaaac ttgccgcaaa tagcgcttta ccttgtccat tcgtatggtt aactaaatca
    13021 gcgatatcac tgtgtttaat acttgcacgt tcggttgcag taatcggtaa ttcaaatacc
    13081 atttcatcga cttcagaact tatttgtaat atatcgttta atatcacttt actattcgat
    13141 tcaaatgcag cagctttatc atcgcctagt gcaacaattg ctgcacccgt taatgtagca
    13201 aagtccataa tcacactagg ctgatattga tttgcataaa acacagcatc tgcaaggact
    13261 aatctacctt cagcgtctgt attcattact tctacagttt caccacttaa tgctgtaaat
    13321 acatcatctg gcttcattga tgcttcattt atcatatttt cagcacacgc aagcactccg
    13381 acaatattta caggcagttg taaacgacta gccgcttcaa tgataccaac gacattcgca
    13441 gcgccacaca tgtcaaactt cattgtagcc atgccattct tcgttttaat actataacca
    13501 ccagaatcat acgttatacc tttaccaact aaggcaattg gtgcttcatc tttgtctttg
    13561 ccattatatg tgatggttac taatctcggt ttatgcttac tacctttacc tactgcttgt
    13621 aaaagtccga atccttcaga aactaaagtg tcataatctt taacatctac tttgaccttt
    13681 gtatttttaa aatgattaac aatatcttct gcaaatgttt gtggtgttaa tacattcggt
    13741 ggcatattac taaagtctct tgccaaatta atggattggc caatactgat accctcatga
    13801 ataaaatcta attcaattaa tgattcacta ataaggttta aattcgtctt aaacggtgcc
    13861 ttcttacttg atttataatg atcgaattca tatgttgcac gctcactttg aataccgcat
    13921 gccattaata catctgataa ctgatcatat tttgaaataa atgaatccat aagtaaatac
    13981 gtatcttcaa tgtgttctga ctttatgtat tggaaaagat gtccccatat tttcaacata
    14041 tcttgataac tacgtgtctt taagtttcct aaaccaactg taattaaacg atatgtttga
    14101 tcttgtacat caaatgcagt tgtataaatc ttcccaactt tactaccaat aatatgttga
    14161 tgttttagtc tttcaagtga ttctgtaata tcgatatgat taaaactaat gcgctctaac
    14221 tgatttaaat gttctggtat accaataatc aatgtattta tttcgttgct tagtgtgtta
    14281 tttaatttaa aattcataat gtacctcctt agatttgata tgttactcac ttcaaattgt
    14341 acaacaataa agcccttcag tgacactgaa ggactttata aatgaataat ttaattttat
    14401 gtgtttagct gaaactttat ttaaagccta gaatttacct tttttgaatg ctaaaccgat
    14461 accaccgatt ttgaataccg cacgtgtatc aatcactttt ttcatgaatg ctgctttttt
    14521 accagcgata ggtttatcaa ataccatacc tacaccgtca tgtgaaccta aagaacaaac
    14581 agttccacga tcaacgtatt cgaattcttc agttgattca ccgtttaaga tgcgtttaat
    14641 gtttttagca acactttcac cttgttgcat tgcaatttgt gctgtagttg gtaatggacg
    14701 ttcttctcca gctgggataa acgctgaaca gtcaccaata acaaaaatgt tgtcgtaacc
    14761 attgattgtt aaatcttgct ttgtaacgat acgtccacgt ttaacgcctt caaatgattc
    14821 ttccattaat ttactaccac gtacaccagc tgcccatact gaagtacctg catttaattg
    14881 ttgtttttca ccatctactt caactacaaa acctttttcg ttacaagcaa cgattggtgt
    14941 agcaatttta aattcaacac cgcggtcttc taagtagcta actgcgtggt taactaattc
    15001 ttctgagaac attggtaaca ttttaggtgc tgcttcaaca caagtgattt taactttatt
    15061 ttgatccaca ccatatttgc tacataattc aggaattctg tctgttaatt cacctaagaa
    15121 ttcaacacca gtgaatccag caccaccaac taagatagat aaatcgttat catctttttc
    15181 ttttgatgct gcatagttag caaatttgtc ttcgatatga cgtgataatt cacgtgctgt
    15241 gataacattt tcaatttgga aagcatgatc tttcatacct tcgatgccga atgtttcact
    15301 aacgaaacct aatgctacta ctaaaatatc aaagtcataa ataccttgat ttgtttctac
    15361 ctttttagca tcacggtcaa tttttgttac ttctgcttga acaaagttca ctttgtcttt
    15421 cttcaagaca ctttccacag gatataatac atcttcatag tttagtgtac ctgctgatgc
    15481 ttcatgtaac catgttgctt catagtgata ttcattttta ttaataagcg taatttctgc
    15541 ttcttctgtt gatatcgctt tttgcaattt agttacagtt tgtaaacctg cataaccagc
    15601 accaagtaca agtacttttt tacgatcttg agccatttaa tttcacctaa gctttcatat
    15661 ttttttaacc aaatgctgat aattactagt aacaactggt cattactact tttaccacaa
    15721 tgtatactag tttatcatca ttttgttatt taagtctttt aaattatgta aaaacctgtg
    15781 atgttcacaa aaaagacatt tttaatccaa tactaattct atagctttta tgttatattt
    15841 tcaagcaaga ttcagtcaga ttgtgaaaaa attaattaat tttttcgtag cctgatttcg
    15901 attaaaaatg tatccgctat cacgatcatt taaaatacta tttatagtca gattcttaat
    15961 tttcaataat ctttttaata aaacaaaata tctttacgca aaaataggac ataatcaaat
    16021 atgtccttaa tcgttattga tatcaatttg tatcccaaaa attgggttaa ccattaaaaa
    16081 gttattttat tagcaatttt caggattacc tgcaactttt gcagttctaa atgaactacc
    16141 acagccacat gaagcaattg cattaggatt gtcgatttgg aaaccgccac ccattaatga
    16201 ttgcttaaaa tcaatagtcg taccatttaa tacgggtgca tcttttttgt ctactaatac
    16261 ttttaatcca aagtattcta agacttcatc attttcacca ggcgcttctt ctgcacccat
    16321 accgtatgtt aaaccagtgc acccgccacc attcacttta atttttaaat agccatctgg
    16381 catttcattt gctttaagca tatcttttac ttcgtaagca gctgcttctg ttaatataac
    16441 tgttggcatg ataactcctc cttaaaaaat ccaagtttct tttatatgtg catatatatt
    16501 ttgtaataat tcttccggcg aatcaccttc aacaatatca ccatttacta aagcatacaa
    16561 cccggctgaa catataccac aatgtgtcag gcaaccatac tctaacacat cgacatctgg
    16621 gtcattttcc agttgattaa aaacataatc tccacctttt gccatgttag agagacaaaa
    16681 ttctacgatc ggattcatac ttcaccttct tatttcattt gttacaatat tatagcattt
    16741 taaaactggt attttaacat gatgtgctca attagcaaca actgatgttt cttatcccag
    16801 ttatgtaata gtgccttagt tagtacaaat atttatttgt agtcattttc aaaaaaatat
    16861 ataataagca tataaataca tatttacttt tttaggggtt attgatgaat caaagacact
    16921 tgcattttat aactgttcat taacaaatat atctatgcac tttgatcttt tcaaaattaa
    16981 agtaaatatt aacatataaa aggggtactt cattatgaaa aacttagttt tgttaggcgg
    17041 cggatatggt aatatgcgta tcatgtcacg cattttaact acttctttac cacaagatta
    17101 tacagtcaca ttagttgatc gtatgccatt tcatggattg aaaccagaat tttatgcttt
    17161 agctgcgggc acgaaatcag ataaagatgt tcgtatgaaa ttccctaatc atccacaagt
    17221 gaatacagtt tatggtgaaa ttaacgacat agatttagat gctcaaattg tctcagtcgg
    17281 taattctaaa attgattatg atgagctaat cattggttta ggatgtgaag ataaatatca
    17341 taacgttcca ggagccgaag aatatacaca tagtattcaa acactctcaa aggctcggga
    17401 tactttccat agtattagtg aactaccaga aggtgctaaa gtcggtatcg ttggtgctgg
    17461 attaagcggc atagaacttg ccagcgaatt aagagaaagt agatcagact tggaaatata
    17521 tctttatgac cgtgggccgc gaattttaag aaattttcca gaaaaattaa gtaagtatgt
    17581 tgcgaaatgg ttcgccaaaa ataatgttac cgttgttcca aattcaaata ttaataaagt
    17641 tgaacctggt aaaatatata actgtgatga acctaaagat attgatttag ttgtatggac
    17701 agcaggaatt caacctgttg aagttgttcg taacttgccg attgatataa atagtaatgg
    17761 acgcgtgata gttaaccagt atcatcaagt accaacatat cgtaacgtct atgtagttgg
    17821 tgattgtgct gatttaccac atgcgccaag tgctcagtta gccgaagttc aaggtgatca
    17881 aattgccgat gtgcttaaaa agcaatggct aaatgaacca ttacctgaca aaatgccgga
    17941 actaaaggta caaggtatcg ttgggtcatt aggagataag caaggttttg catatatcat
    18001 ggaccgcact gtaacaggac gactcgcttc tattttaaaa tccggcgtac tttggttata
    18061 taaatatcat aatggttaaa attaaaaagc aatgtcagtt cacactatta ctgacattgc
    18121 ttttagttat gtgtatatta ttcattcaca agcttttggt ctataaatcg agtgatttgc
    18181 tttgtttgta tataaccatc tgctacatat tcatcgttca ttgtaattaa tggataaaat
    18241 agttcatctt gttctattct ttcaataaat tgtaaatcat gatctgttaa gttgtcatta
    18301 tcttttgtaa tatctatata cgtatattta aatgatatat ttgggtattt tctttttaat
    18361 agcggctgta gccagtcata aatatctttc gatgttggcg cattaacgca acttgcacat
    18421 ataacatctg ccccatatac taccacactc acgtgctcca tatatttatc ccccattggt
    18481 ttgatagatt tttattacac tatctattat aatatactaa taaagattat caaattcatc
    18541 tctcgaaagg agacttgcct gatgcctact gaagatacaa cgatgtttga tcaagtagca
    18601 gaagttattg aacgtcttcg tccattttta ttacgtgatg gtggcgactg ctcattgatt
    18661 gacgtggaag acggtattgt taaattacaa ttacatggtg catgtggtac atgcccaagt
    18721 tctacaatca ctcttaaagc tggtattgag cgtgcattac acgaagaagt gcctggtgta
    18781 atagaagtag aacaagtatt ctaatataaa gatattaagt taacagaaca tattatgaat
    18841 aacaacaaag acaggaatac tgaattattt ataatcagtt attacctgtc ttttttcgct
    18901 gtcaaattgg actgatggga aagttatttt atactattta tctgattaat tattttatta
    18961 ttgccacata tccaagcaca tattactatt ctattctatg aaatttaaaa tataaaaata
    19021 gcacatacgc tcaatcaaaa tcgttgagtt atgtgctatt tgtattttaa tttttaggtt
    19081 tatctacttc aggttgtggt tcacctttca tatgtttcgc aatttgctca tccatataaa
    19141 cccaaccttt ccaaccgatg tgtacggcat cactgataac atatttttca taatctttat
    19201 cagtcatatc gtaaatttta ccaccattat ctacaacagt agaatggatt tttttataaa
    19261 ctgcttgacg acgttcttta tcgataccaa tgtggtcata ccatacaccg tttgatggaa
    19321 tacttacata ttgaacatct gcaccagcag cacgcattgt ttttacaagt aattctaaat
    19381 cttggaattc tggagaatta acattgaatt cgtagtcacg tctaacttta cgcttacttt
    19441 cttgaattaa tttccagtat tgatctctaa taccaaattt attcgatgta gtatcagctt
    19501 taccaatttc aactgctttt tgtttcatct catcccaaga agcgtctggt tttgtagcag
    19561 gtttaacatg ttctaatgga gatttatcca ttgcaaacaa tgatttaatc gcttctattt
    19621 taatcaattg attctcttta aaaccagaaa tataactatc tttagtttct ttagggtttt
    19681 tagcataaga tttcaagtat tctttattgt gtacatgtgg aaactgtaat aaacgttgtg
    19741 cataacgacg ttttaattca gtagacatgt ttttctgctg gaacatttga ttaatttgag
    19801 tttgagacat acgagcatca aagttttgat tcgttaaacc atggtttgta aaccattgtg
    19861 gtgaaataat aaatgttaat ttcttacctt ttaatttatc atactgtgat gcaagttcaa
    19921 ctgcgttaat taagtctgta gaaccaccag cacctaataa gaatgctttt ttgttggcgt
    19981 tatgcttatt taatgcaatt gcaggattaa atgggtcatc tttacctaat tcactagagc
    20041 cgtatatagg ataatacttg tttgattcgt ataacttatc ttgaatgagt gtgcctttta
    20101 gtacttgatc tgtcaatgaa gttctattat cttctacagt cttttcattt actaatcctg
    20161 taaaccaact agcaggtaat aatagaaaga caatgaatac cgctccacta attaaaatgg
    20221 gtaaaaaagg ttttaatttc atcgtaactc ttctaatgct tcaacgattt tatttggtgt
    20281 tgcccactca tctctatcaa agtccataat agatacttcg atatcaagtt tattttgaat
    20341 ctctaataat aatccaactg tttggaaaga atcaataata ccttcttcaa aaatttctac
    20401 gtctggattt tcttttacaa tatcattttc tgctacttct gctaataaat ttaatacttg
    20461 ttctctaaat tccataatta aattctcctt tattatataa gtttacctga gaagattaaa
    20521 aagccaaatg ttacaaagtg gaatgtaatc acaatgctaa gtgctgttgt gaaaccattt
    20581 tgccaacgtg gcggatgttt cttacgccaa cgttcataat agccataacc tataaacaat
    20641 gctgcatggt ataaaccata aacaatgtaa tacacttcga taccatgcca aattcccatt
    20701 atgaagaagt tgattaagaa tgccacgtta gacattgcaa attgactctt caataatttt
    20761 ttacgagaca tgtagaataa agatctcatg taaatacaat ctctgaacca gaatgataat
    20821 gtcatatgcc atctattcca gaaatcttta atatttttcg ctttgaaagg tttatcgaag
    20881 tttggtggtg ttttaatacc gaataaataa ctaaatgcta tcgcaaataa actataacct
    20941 gcaaagtcaa agaataaata taagctgtat gcgtacatat ataaccacaa attgacaaag
    21001 ccatgtaagt ctaattgtaa cggcatgatt gcatatgtgt taataaagta agcaacaata
    21061 tatttataca agaaaccaag cataatcatg tgaattgctt ttaatactaa ttcacgatat
    21121 tcattgcctg ttggtacttt tttatcgtct ttaacgaaac gtttgtaacg atcgattggt
    21181 ccagatgaaa tcgttgggaa gaatgaaata aattgaatta atttccatac tttaatttct
    21241 ttgatagaac catcacgaat ttccataatt aactgcacac ttttgaatgt aacataagaa
    21301 attcctaaga aaccaacaaa ttcaattaat ttactttcat ggaagtgaat ctgatgtcca
    21361 cctaaccatg tactttgtaa cactttcaca agtgctaatg gcaatattga taaaaccatt
    21421 acagttacaa attttgaaaa tgaattattt tttggttttg aatgataata aaacattatc
    21481 aataaaactt gccatacgac gtaaataata aaactaatta attgaacact taaatacttt
    21541 tggtcaaaca gattatgttt atcagaagaa aagattaaca caatcataaa tgctgtaact
    21601 acgccattat aaatgtaact tcgcttacct aaaaatccaa gtataatgac tggtaataat
    21661 gcaattaaag caattaagaa gaatgtaaaa tcaccatatg gaatcatccg ttaattacct
    21721 ctgcaatttt ctttctgtca attttaccat ttgaagtcaa tggcaattgt tccatccatt
    21781 caaactttct aggaatcata tactctggta agcgtgattt caagtcattt ttaatatttt
    21841 tagtcatttc tgcattgtcc gtaacttcag tcgttggcac aattgcacca attaaatgaa
    21901 taactttatc atttttatat acaggtacaa caatcgcttc ttttacgaac tcagactggc
    21961 gtaattgtgt ttcaatttct tctaattcca ttctgtagcc attcaatttg atttggaaat
    22021 caatacgacc ttgaatgaac cattgaccat tttcaaactt cgctttatca ccagtgtgat
    22081 atgtacgaat accgtcatcg aaattaaata cttcagctgt tttttggtca ttttttaagt
    22141 atcctaaact tacactttga ccttcgataa caagttcacc ttcatctgta gtagataatc
    22201 ttgcgcctgg tctttcaacg ccaacaggta atgtcggata ttgatctaag atttcttgtg
    22261 taatttgaat acttgtaact gctaccgtag cttcagttgg accatatgtg ttgtaaatcg
    22321 tcgcacttgg gaaacggttt actaacgctt ttgctgctct gtgaggtaga atttcaccac
    22381 agaagaagaa ttcgttaaga ctaccatatt gttcttcatt aagcgttggt aataataaac
    22441 acatttccat aaatgatggt gttgataccc aaatgttaat cggtgttgct gttagcattt
    22501 catttaataa tttaggttta ttaatcatgt ttttatctac aagatttaat gtaccgcctg
    22561 atgctaaaca tggataaata gccattacag ataaatcaaa tgaaaatggc gcttggttaa
    22621 gccattgttg ttcatttcct gatttattaa gttctaacat ccactcagta aattgaacta
    22681 aacttgcata ttcaatttga acacctttag gttccccagt agaaccagat gtaaagattg
    22741 tgtatactgt gtcgttatct ttaatctgac tatcaaaaat tactgggtct tgagatgttt
    22801 taatatcttc tattgtaaat acttcgcctt ctaaactttc aaatgattca tcagtcgtat
    22861 taaatacaaa ctctggttga accttgttaa taatcatttt aatacggtct tcaggaattg
    22921 aagtgtctac aggtacatat ccacatcctg ctttaatggc accaatcatc ccaacaatca
    22981 tatatggtga catgtgaccg aataaaatca tcggtttctt actaccttgt aatcgatgtg
    23041 ctaatttact agactcatcc attaactgtt gataagttaa ttcatcagtt gtgtgtctaa
    23101 cagcaatgct ttgtggattt gcatccgcaa acgcttgcag cttgttaata atatctgtca
    23161 tattaagtct ccctcattag aactcattat aaatgaagtt attgtgtgtg tcgccactgc
    23221 cataaattaa atataaagta ataaatattg ccaaatacaa tagtgttaat aaatatggtt
    23281 tgaatgcttc aacatattta ttaggtggct gtttactttt agatttcata ttgcacctct
    23341 taaagttctt agtaaaaacg cctttataaa gaccgttcaa tataaaatac gttttaaaat
    23401 ttgtttttta caattcatta tatcgatatt cataatgaaa ttcaatttta attttataga
    23461 ttcaacatag taattggttg tcatcactca attattgtaa atgatacact ttgttgagaa
    23521 catcattcat tttaaaggtt atttaaacaa taaacaatta cagtctatat aacaattttg
    23581 ttatatacgt caaaatcaaa taaactcatc acattaatat gacgagttta taatgttatt
    23641 gaattatcat cagcgcaaat atatacattc gcaagtcaag cataacatat ttaacaattg
    23701 ctttgcttgt tttaccaatg attaaaaacc atacttattt tcaatttact ggagtatgtg
    23761 gtacctgatt tgtcataacc gcttctatat tattaataca taattgaatc atattgtccc
    23821 gtgttgttac tgatgcgcta ccaatgtgtg gtgtaatcag aacattatca cgtcccatta
    23881 atggatgtgt atgatcaatc ggttcatttg ctaatacatc taaaccacat gctaaaattt
    23941 ctttattgtc tagtgcatcg attaatgctg tttcatctac aatttgtcct ctaccgatat
    24001 taataaaaat tgcatcattt ttcatttgtt caaatgcttc agcattaaat ttatgatgtg
    24061 tttcttttgt aagtggcgct gtacagatga taaaatcact ttctgctaac aacgtttcaa
    24121 aagaaacata tgttgcatta aagtccgcct ctgcatcttt atgtcttgat tgattatgat
    24181 aaagaatatt agtattaaac ccttgcaacc ttcttgcaaa agctttacca atatctccca
    24241 taccatatat tccaatagtt gaattgaaga catctttacc tgacaataaa taaggacccc
    24301 agctttgcca tgcatctgct tctacatatt tttcagcttc tacaatacgg cgtgctatag
    24361 caagcattaa tgtaaatcct aattctgcag ttgtttcagt aagtacattt ggtgtattcg
    24421 tgacaaccac gttattcgct gttgcacttt caacatcgat gttgtcatat cctacagcca
    24481 tattcgcaat tacttttaaa tttggtgatt gcgctaaaat ttctgcatcg atctgttcac
    24541 ttaatgtaat aaaacaagca tctgcgtcac gaagtgctgt cacaaattga tctttaggca
    24601 taggcactaa tgatttttgc cacataacaa cgtcaccaag tttacttaat tgttgataaa
    24661 atttatctgg aattttcctc gaaacaacta tttttaccaa atcaattcac ctactttata
    24721 aaatgactat atctattttt ctaattcagc tattgcttca tttaaatctt taaaagaata
    24781 cgttggtggc acatttttat tttgcacatc ttctaacgta gatacacctg tttgtacatg
    24841 aatcgtatcc atacctacgt taataccaga cataatatcg gtatcgtaca gatcgcctac
    24901 catagcaact tcggatttat ctaatcctaa aatttctaat gctttaacca taataatcgg
    24961 ttctggttta ccaataaatt gtggcgatac acctgtcgat acacttacaa cacttgtaat
    25021 agcaccatta ccaggtaata aaccacgctc tttaggaatt gatacatcag gatttgtaga
    25081 aataaatgtt gcaccatttc ttacacctaa cgttgcaatc gcaagctttt catatgtaac
    25141 ttgttcgtca agtccaataa ctacataatc aacatgctcg tcatttttaa tgacaagtcc
    25201 cgcttcggtt aacgcagtat ttaaaccact cccacctaac atatatactg atgctcctgg
    25261 tgattgttct gaaatataat cagcagtggc taacgctgac gttacaacct cttctggttt
    25321 agcatcaatg tgcatttcac gtaatttttc agttacttgc tcaggtgttt ttgttgaatt
    25381 attagttacg tataaatgag gaatgccttt tacatttaaa taatcgatga attgttttgc
    25441 tccatcaatc tcatctgttc ccatatacat tgtgccatct aaatcgatta aatacgcttt
    25501 atactgtttc attattgatt ttctccttta ccaaaagctg ttactggtac attttcttga
    25561 tgtaaaaatg tcgttacttc tgtaataaat tgtttgtaat aaggtaatgc gtcatcaaac
    25621 attggcatga taatcttaat atctaaggct gtgtaatcat atgtaaattg tttacgaata
    25681 ccgacagttt tattaatcgc ctgctgtgtt tctttagtaa taacattttc tagttctaat
    25741 atatcaatca catctttata attaccagga tcccttaaaa taaatgcatc gataatcata
    25801 ttccctatat ctaccgatga ttctatcaac atttgagcaa tgcgttcaaa tgcataatga
    25861 ttgtgcttgc tctcatgata atcatcagtt aatgcttgta aataggctaa tttctgagtt
    25921 agtttatctt tgtctacaaa atacattcct tgtcacctct cacttatata ttatcataat
    25981 tttgcagtat acttcatttg tcgttatact aactttacta cttttaaaag gagtcgctac
    26041 aaaatgattg atatgtattt atatgatgac aacgaagaga gtcaagttca gtttgttggt
    26101 tttgttggcg agcatagtcg atatgatttg atgttagtcc atacaaatag acattatggt
    26161 aagacactcg tacttaatat gcaaacaaat aaattcggta ttatcggtac tgatgattta
    26221 aaagaagaag gctatatcgc tcatatttta ggtgtaaatg ctgaagaagg cgatgaaatt
    26281 actgagtatt taaatgaagt cattcattaa agataaaaac ccctaactag attacaggtc
    26341 catgcttgaa ttgcatgatt cccattttca atctagctaa gggttttatt aatgttcagt
    26401 atcttgaatg gttggcttaa tcttcacatc ttgaagttta ttgtcgtcat ctatcacatc
    26461 agacggatga acttcttcga ttaacttttt aacctcttgt ttagacaaat gtcttaatac
    26521 aaagtaagga caaccaaagt tacaatattc aaatatgtaa tcttgaatat ttgaaaaacg
    26581 tttactcatc tctgcttttt tattagaatc tttgtaaaaa ccttttaatc gtaattgatc
    26641 gtaaccatag tcaccaacta tgtaatcata tttatctaaa atatctgaat acctagcaat
    26701 aaattgttct tcattaaaac attcgcgata attttctatt aattcaaagt aatgttgatc
    26761 tacttttatc aaattcttca cctatctatg acaatgaaag gctcagttta tacttttagt
    26821 gtttcataaa aataatgatt acttctatga cacgttcgtt ttgtacaaac taagccttta
    26881 tgccttatta atggttaaat attaactatt taactgtgcc tcaccttggc gttgcttttc
    26941 tttagcagct tcatttactt gctcatccgc atgataagaa ctacgtacta aaggtccagc
    27001 ttggcaatgt ttaaaccctt tatccattgc cacttttctt aatttaccaa actctaaagg
    27061 cgtgtaatat ttttgaacct ttaaatgttt acgtgaaggt tgtaaatatt gaccaatcgt
    27121 taaaatatct acatcattcg cacgtaaatc atccatcgtt tcataaattt cttctatagt
    27181 ttcacctaat ccaaccataa tacttgattt agttgggata tccggttgta attcttttga
    27241 acgacgtaaa aactctaatg ttctgtcgta agtcgcacgc gcacgaactc tcggtgttaa
    27301 gcgacgaaca gtttcaatat tatggtttaa aatgtcaggt cttgacgcca ttaatgtttc
    27361 taacgcatca tagtccccgc ccatatctga tggtaaaatt tcaatcgttg taaatggatt
    27421 tctttctcta actttacgta ctgtctcagc ataaacattt gaaccagcat ctcttaaatc
    27481 atcacgcgca acagcagtga taacaacgtg tttcaaattc attaattcaa ctgattcagc
    27541 tacacgttca ggctcattta aatcaagttc attaggtaaa cctgtcttaa ccgcacaaaa
    27601 acgacaagct cttgtacata cggcacctaa aatcataaat gtcgctgtac gacgtgcacc
    27661 ccaacattca tgtatattag gacatttagc ttcttcacat acagtattaa gatttttttc
    27721 cctcatcatc ttcttaagtc ctgtatagtt ttcgttggta tttaatttta ttttcaacca
    27781 atccggttta cgtaatattt cctcgttttt tgtcgccata acaacacata ccctcctgtt
    27841 aagtgtatct ttaaacatta tatcgaaatt catgacaaat gaaaatccaa taatcttact
    27901 ttttaatatt ccaataattt ttctttaaat atatctctta aaaactctgg cattaaatac
    27961 tcttttggta attctttcaa tgttgggaaa taacgaccga acgtatacat atctatcgta
    28021 cctagtacat attctttgtc gtcttcgatt tcttctccat tcagatagta acgccctgta
    28081 gaatgaatgt accctaaatt ataaagaata tatcctccaa atatattccc tctgaaaccc
    28141 aaaccttgtg catgttcata catatattct tgtttattac ttttagctat aatctctttt
    28201 aatttcacac caaaaagtct aacccttacc atattaatcg gatgcggtaa catttgatga
    28261 atgtcatatt ctgtcacttc atcttttaca atatctttaa cgagtaagcc agcattgatg
    28321 atggcacatt gtgcatgtgt atactcacaa acactttgag ctaataagta tgcagcttca
    28381 gttatgtgat tcatactacg cttaagcact actggatgtt gaattactga attggacatt
    28441 aacgtttttc cttcttcttc aaatgaagtt tcaacttcag gtaatgtttt taaaggaata
    28501 atctttgcag ttttatgtac tactttatgt gcctcaaaag ttaaattaac ctctccaaga
    28561 taattaccat actttccagc tgccgccatc agtacaccat tattgatttc accatgttcc
    28621 aaataatgat gcgtatgact accaaaaatg acatcaattt caggcaattc ttggcataat
    28681 gtttcatcga agaaaatgcc acaatgactt agcacgatta atacatcaaa tttacctcgt
    28741 tgaagttcaa tttcttcttt tatagattca agtggatcgg taacaatcca atttagtgca
    28801 cgataaaatg gggtaaaagg tgctgtcgct gcaacgaata gtattttcac accgtctatg
    28861 tccttaatat aagaagaaac gatattattt ggtaaatgac ctgattcatc tataacattg
    28921 ctacatgtca ctataaattt tgcttcgtcg taaaggtgat ttaaagcttc gtgtgaaatg
    28981 gtcatccctt cattattacc gattgttgca acatcacatt ttgcttcatt tagtaatgcc
    29041 acattctttt tacctaaagt tgcttcagtt ataggtgcgg ataaatctac atgatcacct
    29101 agatcaacat ataaagaagg atgattaagt cgtggccgat gttctgccat atatgcttta
    29161 atgcgttcgt attcatgtaa atgactatga atatcgttcg tatgataaat tgtaagcctc
    29221 aaattgacgt cctcctttta aagttgtatt aaatcaacgc acgaataatt aaatatatac
    29281 ccattaacaa cattactgtt ctcaataatg ttactaccgt atctgactta attgattgat
    29341 tcactttgac accgattttc gcaccaaaat aactagaaat aattaaaatg attgcataac
    29401 cccaagctac gtgaccttga gcaatgtgcc ctatagaact catgacactt gaaaagaaaa
    29461 tcatcatcat acttgttcca acagctacat gaggtggaaa tctaaataca ataagcatta
    29521 gtggcgtcat tagtgcgcca cctccaatac caaataaacc tgtcaatata ccaattaaaa
    29581 acgttgtaat aaaagcaaac aatggtggaa cactataatg atatgtttta cctttagcgt
    29641 ctacataagt cttttcatac ttgggtttat cgaaaatttt aaacggttta atcttatttc
    29701 ttaccattaa taaaatggct acgaaaatta aaaagatacc aaaatataaa ttaaatgact
    29761 caaatgttaa atatctacta atgaaggacc caagcaatga acctggtaat agtccaaata
    29821 aaaagatgga accattttta atatcaactt gttttgtttt taaatatcca agtgatgaag
    29881 aaagtcctgt cacaattaga atgactgaag acgtccctat cgctacttgt gttgtaatat
    29941 tatgtagtaa tccatgttca acaccgaggt aaaccattgt tggaacgata ataataccgc
    30001 ctccaatgcc tacgatagac cctataatcg ctgacaaacc tccgattaaa actaataatg
    30061 taattgttaa taacatagtc gtcgcctctt aaaataattt taattgttgt ggtgctaacc
    30121 cttcatattc tatattcaat aatcgctgat atgttttggc attatttgct gcatgaccac
    30181 cagaattatt gttaaatatg acgtaaactt tcttagcttt ttgtgctaat atttgtgcct
    30241 tttgtgccaa gtctattaat tcttgctcat tataatcata taaatagcgt acatcgcgcc
    30301 attcttgatc tgacatatct ttcttagtcc aaccgtaatg attacgtcca tgataacgta
    30361 caaacgcaat ttcatttgtg attcgattga ctaaaggtac actgccatct ttcacttgtg
    30421 gttcatcaac tactgcatga atgatttgat gttctgttaa aaatgccaat gtttgttctt
    30481 taaatgcatc actaaaccat gattgatgcc taaattctac acacattgga aatgcttgta
    30541 attgctgtct tacatacaaa atatatttga tattttgtgc attgcagtca aaccatggcg
    30601 gaaattgaac caataccatt gctaattttt tctgtgtatg taagggctct aacatattct
    30661 taaattgatc aaatagttct tgccttgtat ctgcaaatgt tttgtagtct gcatgcaatg
    30721 tgagtgcttg atgaattttg accacaaatt caaatgtatc aggcgtttct tttatccatt
    30781 tcaatatatt tctttccggt tgtatcgcat agtatgtcgc atctaattcg acaaccggaa
    30841 aatgtccagc atatgtttta agtttatcgg tttggcgttc taaatcttca tataatgaat
    30901 agtgatcacc ccaacctgtt aatccgatgt ttatcatata tatcaccaat gtcatcatac
    30961 catataactt ttatcataat catttcagcg aactttagtt tgataatttc gtttgactag
    31021 attggtaaaa tagaaacgca atccacaaaa atggcaagca ctaaaataat gtttgggggt
    31081 gcttgtgctt ttgtggattg cggtcgattc gattatttat attgcatgat ttgattaatt
    31141 tgattgatta tattggacac gatggtgttg gcgggatgcg ttgttgctag tcgcgggctt
    31201 tgtccactcc acatatgtgt taactctttg tcgccgatgt ttgccgcggc ttttcttatg
    31261 ctacttgtta gctcattttg tattggataa tctgggatgt cgccttcgta ttgggacatt
    31321 tcttcgataa acctattgtt gataccgcgt gcaagctttc cactaaacgc ttttgtaacg
    31381 actgtatctg tttctttact atttataatt gcgtctcgta gtagttctga tgcattacta
    31441 tcttgtgatg ttaaaaatgc agtgcccatt tgtacccctt ctgcacctaa gacaatactt
    31501 gccaaaactc ctctaccgtc cataattcca ccagcggcaa tgaccggaat tgaaacgaca
    31561 tctacaattt gtggcactaa agatattgtt ccaaccatag gtaattgatt tttaggtttt
    31621 aaaaatgaac cacgatgtcc acctgcttca ctaccttgag caacgatagc atccataccc
    31681 gctttttcat tcgcaatagc ttcatcaaca cttgttgctg tacctattag cttaatgttt
    31741 gcttctttca atctttttat aatagattcg cttggaattc caaaagtaaa acaacataca
    31801 ggcacttgct ttttaattat cgtatcaata tgacacttaa attgttgttc ttcggtaatt
    31861 tttacaaccg gctcttctaa atgtaatgcg cgtcgataag gttttaacca tgcattcata
    31921 ttttcaattt gactactagt atatgattgt tgacttggta caaagacatt tacgccaaaa
    31981 gaatttgacg ttaattggcg tacataatct atttcatctt ccaattgctg cgtattaaag
    32041 taacctgcgc ctattgtgcc taacccacca ctgttactta ctgatgcaac taatttcggt
    32101 gtcgtacttc ctgccatacc tgcttgtata attggatatt caatacttaa catttgagta
    32161 agtcgattct tattccacat agctgttcgc tccttatata gatacgttgc gatttttccg
    32221 ttgttgaaat tgaatttgct gttgagaaag tttttctttt tcctttttat ccatctcatc
    32281 ttcaatttcc atacctaata attcttcaat taagtcttca tgtgacacta tcgcttcagt
    32341 accaccaaat tcgtccaaca caattgctaa atgttttcta gaaatagtca tcttacgtaa
    32401 tacccattca gctttattgt gttcattcac aaataatggc ttagctgaat agtttgtaat
    32461 ttgattttct tttttattac tccaagccaa cagatattta gaatgaaaca ctccaataat
    32521 gttatcaata tctccctcgt acactggata tctagtgtat ggcttattca taaccgtttc
    32581 ataaacttct tcgtatgtcg catttgaagc aaatgccgtc acattaattc taggtgttgt
    32641 atctacatct tttactttta aattttcaaa attaatgaca ccttccaacc tactcgtctc
    32701 aatttcattt aaagcacctt catgtccagc aattgctaac attgttttaa attcttcttt
    32761 tgaaaattga tgttcttgag gttgaccctt agataaactt cgattaatac tgtccgtcaa
    32821 cttatttaaa agtaatgtga tagggcgaaa cacaatgaca caaatattaa taattggata
    32881 tacaagcctt gttattttat ctggaaatgt tgcagcgaca gacttgggaa tcacttcgga
    32941 aatcaaaatg ataacaactg ttaaaacagc tgatgcaata ccaacgctaa tcccccaacg
    33001 taaagccata attgtaacaa gtgttggtaa taaaatattc gcgacattat tcccaattag
    33061 aatcgttgta ataaactcac ttggtttttc aagtaacttt acaatgcctt ttgctttttt
    33121 atcacctttg tcagcttcag ttttaaattt tgttttattg gcagccgtta atgccgtctc
    33181 gcttcctgaa aagaaaaacg aaataaatat caatataatt atggcaatga tcacgcgtgt
    33241 agtctcctta ttgtcatatc ttatttttat tgtagttact taattcccga cgttctcgtt
    33301 t
//
LOCUS       DAMZKC010000010        32438 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.010, whole genome shotgun sequence.
ACCESSION   DAMZKC010000010 DAMZKC010000000
VERSION     DAMZKC010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 32438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32438
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.010"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(291..818)
                     /gene="isaB"
                     /locus_tag="POB55_000351"
     CDS             complement(291..818)
                     /gene="isaB"
                     /locus_tag="POB55_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunodominant staphylococcal antigen IsaB"
                     /protein_id="HDI6499347.1"
                     /translation="MNKTSKVCVAATLALGTLIGVTVVENSAPTSKQAQAAITPYYTY
                     NGYIGNNANFILDKNFINAIKYDNVKFNGIKLAKTNTIKKVEKYDQTFKGVSAKGNEA
                     SQLQFVVKNNISLKDIQKAYGKDLKKENGKTKEADSGIFYYQNAKKTLGIWFVVDHNR
                     VVEVTVGHTPYKTSK"
     gene            1076..1534
                     /locus_tag="POB55_000352"
     CDS             1076..1534
                     /locus_tag="POB55_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499348.1"
                     /translation="MKKLLITIVIIVLIGALGFAIYAAINHSTSSSNNQEQENKATHK
                     KTETEENDQQDDSADKADKNQNNGNNVTNDNQPSSQSNVPNNHSTAPSNTQPAQPKDS
                     NSNKNNTHDGGSQSSTNNQNNQQQNTPPANKSNNTNTSSKQTPQAQSPKN"
     gene            1774..3648
                     /locus_tag="POB55_000353"
     CDS             1774..3648
                     /locus_tag="POB55_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDI6499349.1"
                     /translation="MLDRHLKLLQFFIKNPSKHISSNEIAEHVNVSNRTVRNDIHVIN
                     SNFMDDIIVSIKSKGYQLNTSQYTLETITERYTHIQSYKEKLLLSMAYQLLMHNKSQT
                     LQQLEQDYLLSKTVLNDYFVRIQQWCQKFNIALTIKKKQGIVVDGTDNDITNAIIHLN
                     QLSSGHVHVEDLILNELPDSHQRMISHIIQETLQQHSIETTDIQIQQLLIHLILIIKR
                     KQSLSELDTLNHDSKLISQSCIKQINERLGYDLNEKVINMFSFFIAYHFNKLDIGIER
                     IFIQSYIDRLIELMQQRIHIPFNEDSILQQNLYNHFSKAYLRITQNIYLNNPLVIEIK
                     KLYPFVFNTLFEAIDKLAIDTDIEMSEDEIAFLTIHFQAAIERRTKTQLNVVIACYYG
                     LGVSNFLETKINNLSEELSVINTIKLENITHYHFDNVDLLITTHDIPKQTLNILPKHL
                     TTIKVAPLFSEDDRHKIRLVVKQKQNPVQAHHHMDTVNFLVVNTEQKSRHTVQIFEEA
                     QKILQAHHAIVEGYIESALEREKSSSTYIGNFMAIPHGDPEKVLQSHVLIFRTKDVFP
                     WRQHDVKLVFFLAISHKDKAFTKQMMQLIANLDDDSVNHLCSLDDHSLKQQLFEYLQE
                     "
     gene            3725..5677
                     /locus_tag="POB55_000354"
     CDS             3725..5677
                     /locus_tag="POB55_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HDI6499350.1"
                     /translation="MKIVAITSCPNGIAHTYMAQEKLEQVAKEMGVDIKVETQGGVGA
                     ENVLTTQDIEEADGVIIAADKQVDLSRFVGKRLINENVREGIHNPRGLIQRIINQDAP
                     IYQSETNYHSKDRGKSKSGIQMVYQHLMNGVSFMVPFIVVGGLLIAIALTLGGETTSK
                     GLVIPDDSFWKSIENIGSLAFKFMVPILAGYIAVSIADKPGLVPGMIGGAIAADGSFY
                     GSDAGAGFLGGIVAGFLAGYIAKWIKDIKVPKAMAPIMPIIIIPIISSVVVGLIFIFL
                     IGAPISNIFEALTTWLKSMQGANIIILAMIIGAMIAFDMGGPVNKVAFLFGSALIAEG
                     NYAVMGMVAVAVCTPPIGLGLATFVQKYKFNHSEREMGKASFTMGLFGITEGAIPFAA
                     QDPLRIIPANIIGAMIASVIAAIGGVGDRVAHGGPIVAVLGGIDHVLWFIFAVIVGSL
                     VTMTTVLLLKRNTPVIAVDAPAQHTQLHDTDITQHDTEVDNVDKTAKHSDLNKHSHVF
                     DQQTMIITDHDMSRNEAIDMLIHKLKICRYVEHTSHLKNAILEREMESTTAIGMNVAI
                     PHAKSDVVKQPIVAVMKNNHGVKWDSLDGSLPQLIFLIAVPNNSQDTHLKILQRLSKA
                     LMNDETRQSLINANSTTEIYNLLMKI"
     gene            5691..6629
                     /gene="manA"
                     /locus_tag="POB55_000355"
     CDS             5691..6629
                     /gene="manA"
                     /locus_tag="POB55_000355"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HDI6499351.1"
                     /translation="MALFLQPVFKDRIWGGHALKAFNYDIPNETTGECWAISAHPNGP
                     NTIINGPYKDMTLDQLWSQHRELFDNDSRDSFPLLTKVLDANDKLSVQVHPDDDYALK
                     HEGELGKTECWYILDAAPGAEIIYGVHAHNKQTLIDMIDNHEFDSLFKRIPVKPGDFY
                     YVPAGTVHAIGSGILILETQQSSDTTYRIYDYDRRDQNGQLRDLHLEQSKDVIELGNH
                     EPNTTPITTHIDTHTVTQFVDNQYFAVYKWDIHGDLKFNKPHAYCLVTIIDGTGTLTV
                     NDKHYDIQKGSSFILTTEDEDIHFVGNISAIVSHPG"
     gene            complement(6741..9722)
                     /locus_tag="POB55_000356"
     CDS             complement(6741..9722)
                     /locus_tag="POB55_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HDI6499352.1"
                     /translation="MKNAFKLFKMDLKKVAKTPAVWIILAGLAILPSFYAWFNLWAMW
                     DPYGNTGHIKVAVVNEDKGDIIRGKKVNVGNTMVNTLKKNKSFDWQFVSREKADHEIK
                     MGKYFAGIYIPSKFTHEITGTLRKQPQKADVEFKVNQKINAVASKLTDTGSSVVVEKA
                     NEQFNKTVTRALLEEANKAGLTIEENVPTINKIKNAVYSADKALPKINDFANKIVYLN
                     NHQADLDKYANDFRKLGNYKGDILDAQKKLNEVNGAIPQLNEKAKLILALNNYMPKIE
                     KALNFAADDVPAQFPKINQGLNIASQGIDQANGQLNDAKGFVTQVRSRVGDYQDAIRR
                     AQDLNRRNQQQIPQNSAANNETSNSAPAAGNGVASTPPSAPSGDTAPNNNVTQNTAPN
                     SNNAPVSTTPQSTSGKKDGQSFADITTTQVSTANENTQNITDKDVKSMEAALTGSLLS
                     LSNNLDTQAKAAQKDSQALRNISYGILASDKPSDFRESLDNVKSGLEYTTQYNQQFID
                     TLKEIEKNENVDLSKEIDKVKAANNRINESLRLVNQLSNALKNGSSGTAEATKLLDQL
                     SKLDSSLSSFRDYVKKDLNSSLVSISQRIMDELNKGQTALSNVQSKLNTIDQVINSGQ
                     AILKNGKTRIDRLQTVLPSIEQQYISAVKNAQANFPKVKSDVAKAANFVRNDLPQLEQ
                     RLTNATASVNKNLPTLLNGYDQAVGLLNKNQPQAKKALSDLADFSQNKLPDVEKDLKK
                     ANKIFKKLDKDDAVDKLIDTLKNDLKKQAGIIANPINKKTVDVFPVKDYGSGMTPFYT
                     ALSVWVGALLMVSLLTVDNKHKSLESVLTTRQVFLGKAGFFIMLGMLQALIVSVGDLL
                     ILKAGVESPVLFVLITIFCSIIFNSIVYTCVSLLGNPGKAIAIVLLVLQIAGGGGTFP
                     IQTTPQFFQNISPYLPFTYAIDSLRETVGGIVPEILITKLIILTLFGIGFFVVGLILK
                     PVTDPLMKRVSEKVDQSNVTE"
     gene            9933..11792
                     /locus_tag="POB55_000357"
     CDS             9933..11792
                     /locus_tag="POB55_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HDI6499353.1"
                     /translation="MPKNKILIYLLSTTLVLPTLVSPTAYADTPQKDTTAKTTSHDSK
                     KSNDDETSKDTTSKDIDKADNNNTSNQDNNDKKFKTIDDSTSDSNNIIDFIYKNLPQT
                     NINQLLTKNKYDDNYSLTTLIQNLFNLNSDISDYEQPRNGEKSTNDSNKNSDNSIKND
                     TDTQSSKQDKADNQKAPKSNNTKPSTSNKQPNSPKPTQPNQSNSQPASDDKANQKSSS
                     KDNQSMSDSALDSILDQYSEDAKKTQKDYASQSKKDKNEKSNTKNPQLPTQDELKHKS
                     KPAQSFNNDVNQKDTRATSLFETDPSISNNDDSGQFNVVDSKDTRQFVKSIAKDAHRI
                     GQDNDIYASVMIAQAILESDSGRSALAKSPNHNLFGIKGAFEGNSVPFNTLEADGNKL
                     YSINAGFRKYPSTKESLKDYSDLIKNGIDGNRTIYKPTWKSEADSYKDATSHLSKTYA
                     TDPNYAKKLNSIIKHYQLTQFDDERMPDLDKYERSIKDYDDSSDEFKPFREVSDSMPY
                     PHGQCTWYVYNRMKQFGTSISGDLGDAHNWNNRAQYRDYQVSHTPKRHAAVVFEAGQF
                     GADQHYGHVAFVEKVNSDGSIVISESNVKGLGIISHRTINAAAAEELSYITGK"
     gene            complement(12057..12617)
                     /locus_tag="POB55_000358"
     CDS             complement(12057..12617)
                     /locus_tag="POB55_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDI6499354.1"
                     /translation="MSRKTALLVLDMQEGIASSVPRIKNIIKANQRAIEAARQHRIPV
                     IFIRLVLDKHFNDVSSSNKVFSTIKAQGYAITEADASTRILEDLAPLEDEPIISKRRF
                     SAFTGSYLEVYLRANDINHLVLTGVSTSGAVLSTALESVDKDYYITVLEDAVGDRSDD
                     KHDFIIEQILSRSCDIESVESWKSSL"
     gene            complement(12786..14693)
                     /gene="sasF"
                     /locus_tag="POB55_000359"
     CDS             complement(12786..14693)
                     /gene="sasF"
                     /locus_tag="POB55_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-wall-anchored protein SasF"
                     /protein_id="HDI6499355.1"
                     /translation="MAKYRGEPFQLYVKLSCSTMMATSIILTNILPYDAQAASEKDTE
                     ISKEILSKQDLLDKVDKAIRQIEQLKQLSASSKAHYKAQLNEAKTASQIDEIIKRANE
                     LDSKDNKSSHTEMNGQSDIDSKLDQLLKDLNEVSSNVDRGQQSGEDDLNAMKNDMSQT
                     ATTKHGEKDDKNDEAMVNKALEDLDHLNQQIHKSKDALKYASKDPAVSTTDSNHEVAK
                     TPNNDGSGHVVLNKFLSNEENQSHSNRLTDKLQGSDKINHAMIEKLAKSNASTQHYTY
                     HKLNTLQSLDQRIANTQLPKNQKSDLMSEVNKTKERIKSQRNIILEELARTDDKKYAT
                     QSILESIFNKDEADKILKDIRVDGKTDQQIADQITRHIDQLSLTTSDDLLTSLIDQSQ
                     DKSLLISQILQTKLGKAEADKLAKDWTNKGLSNRQIVDQLKKHFASTGDTSSDDILKA
                     ILNNAKDKKQAIETILATRIERQKAKLLADLITKIETDQNKIFNLVKSALNGKADDLL
                     NLQKRLNQTKKDIDYILSPIVNRPSLLDRLNKNGKTTDLNKLANLMNQGSNLLDSIPD
                     IPTPKPEKTLTLGKGNGLLSGLLNADGNVSLPKAGETIKEHWLPISVIVGAMGVLMIW
                     LSRRNKLKNKA"
     gene            complement(14935..16293)
                     /gene="gtfB"
                     /locus_tag="POB55_000360"
     CDS             complement(14935..16293)
                     /gene="gtfB"
                     /locus_tag="POB55_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosylation chaperone
                     GtfB"
                     /protein_id="HDI6499356.1"
                     /translation="MIKLFDYFNNHSRKLYESFKASKLEEDLTIVLNDNGFLPDDVIS
                     PYQFFADNHNTENMKPRFFNQVTVPAFWEIKGNNNSATINDMGRLRGKIFYQSGERPR
                     IVSRVEWFDDQQRVRFVDYYSKNGIKFAQTVYDLNRKAILKKYMTAEGKEVIYENFVT
                     SDVILDWQGKSYFFPSKLAFVLFFIKQLEITEHHFVINSLALPFSVLYNLPSKGSDVL
                     VWQEHCNGNVPGNMQLMCKGDMKRHCNIIIPDKNEYETMLNIADAKVQSRILQGGYLY
                     NYRSRNRYTKEIVILTNSDQLRNIKVLVETLPDFKFHIAAITEMSDKLMQLDQYANVH
                     LYPSINIDRVNELYQLCDIYLDINEGNEILNAVEQAFDYELLILGYRQTAHHAKVTLS
                     EHLFEHNDEITMESKDQLIQMLESLKDQQQFQDALLAQKAHAHEISREQFEQVFKQAL
                     ES"
     gene            complement(16286..17794)
                     /gene="gtfA"
                     /locus_tag="POB55_000361"
     CDS             complement(16286..17794)
                     /gene="gtfA"
                     /locus_tag="POB55_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosyltransferase GtfA"
                     /protein_id="HDI6499357.1"
                     /translation="MTIYNINFGIGWASSGVEYAQAYRAKLLRRTNQDTKFVFLDFIQ
                     SENIQTLTQNIGFKDQEVIWLYQYFSDISIAPTTYTLDDIERELGHEISFRERNGKIV
                     RLYFNGKSSFVTCYLQNEQKDVVDRAEFVINSMLVRKDFYSYTRIFSEYYAPADNKAK
                     LYMRQFYNEDGSIAYNEYIDGDSSIYVFEDAVLYNKTEFIAYFLQRLNLTRDDIVILD
                     RASDIGQAVLQHKGDSKVGVVIHADHYSNNMMSEQHILWNNYYEYQFSKAKYIDFFIT
                     ATDIQNHMVCRQFEQYQGYRPRVYTIPVGSIDALSYPKLSRKPYAMISASRLANEKHI
                     DWLVKAVIVAKRQVPELTFDIYGEGSEKTRLRKIIDTHRAQDYIRLLGHVKLDEIYND
                     YELFLSASTSEGFGLTLMEAVGSGLGMIGLNVNYGNPTFIRDGENGYLVPFDTDEDSV
                     DDVIAKLAHAIVMYFNNGPQTPHDISYEVAQQFMTQDIILKWETLVQEVLHD"
     gene            complement(17812..20202)
                     /gene="secA2"
                     /locus_tag="POB55_000362"
     CDS             complement(17812..20202)
                     /gene="secA2"
                     /locus_tag="POB55_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HDI6499358.1"
                     /translation="MKHKLDVTINELRLKSIRKIVKRINTWGDEVKSYSDDALKQKTI
                     EFKERLASGVDTLDTLLPEAYAVAREASWRVLGMYPKEVQLIGAIVLHEGNIAEMQTG
                     EGKTLTATMPLYLNALSGKGTYLITTNDYLAKRDFEEMQPLYEWLGLTASLGFVDIVD
                     YEYQKGEKRNLYEHDIIYTTNGRLGFDYLIDNLADSAEGKFLPQLNYGIIDEVDSIIL
                     DAAQTPLVISGAPRLQSNLFHIVKEFVDTLIEDVHFKMKKTKKEIWLLNQGIEAAQSY
                     FNVEDLYSEQAMVLVRNINLALRAQYLFESNVDYFVYNGDIVLIDRITGRMLPGTKLQ
                     AGLHQAIEAKEGMEVSTDKSVMATITFQNLFKLFESFSGMTATGKLGESEFFDLYSKI
                     VVQVPTDKAIQRIDEPDKVFRSVDEKNIAMIHDIVELHETGRPVLLITRTAEAAEYFS
                     KVLFQMDIPNNLLIAQNVAKEAQMIAEAGQIGSMTVATSMAGRGTDIKLGEGVEALGG
                     LAVIIHEHMENSRVDRQLRGRSGRQGDPGSSCIYISLDDYLVKRWSDSNLAENNQLYS
                     LDAQRLSQSNLFNRKVKQIVVKAQRISEEQGVKAREMANEFEKSISIQRDLVYEERNR
                     VLEIDDAENQDFKALAKDVFEMFVNEEKVLTKSRVVEYIYQNLSFQFNKDVACVNFKD
                     KQAVVTFLLEQFEKQLALNRKNMQSAYYYNIFVQKVFLKAIDSCWLEQVDYLQQLKAS
                     VNQRQNGQRNAIFEYHRVALDSFEVMTRNIKKRMVKNICQSMITFDKEGMPVIHFP"
     gene            complement(20192..21151)
                     /gene="asp3"
                     /locus_tag="POB55_000363"
     CDS             complement(20192..21151)
                     /gene="asp3"
                     /locus_tag="POB55_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp3"
                     /protein_id="HDI6499359.1"
                     /translation="MLKNKTFKVTWNYISQTTFMYGSKVSFSNGEVTFINPLMPSGLP
                     IHEWLMLKQFSKYKSAPSLPILRRGQHYKLHFDFDATPAGSVYFIIIFYNKNGTKLST
                     EIVKSNSITIQYPDEAYAYKIKMMNAASTSLVFRCLTITEMTHQDDLEYKSMRVTKID
                     DNQYGNDKINVIIAEPSDVYPTISNDFLKLFGHVWLVERWMEDDIKENIKQLKDDLQS
                     QDTLTAINLISYGSKSNVFATYVAQHLDCKVYRTSHEDDDLKEWLKEHVPGNHELKDT
                     NVEVYFKEEQDNHLNYMSRLMNPVRFLDYLDVSKLNGGEVNET"
     gene            complement(21129..22697)
                     /gene="asp2"
                     /locus_tag="POB55_000364"
     CDS             complement(21129..22697)
                     /gene="asp2"
                     /locus_tag="POB55_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp2"
                     /protein_id="HDI6499360.1"
                     /translation="MPRKFRVLQIGGDDLEPIFQHKKGVSWDYFDIGLFEFDSGYVEA
                     IEAIVEAEGRFDFIYIQAPYSETLTNLLQMISEPYNTYVDESFWSVEYEQDENVQKYV
                     VQPLHYRNIEERNNKLEAVSFSGQYGDKVSPKLALVHPNFKGDVVYQGNSELTLSGEF
                     GKEFKPIASWQNNLVYDKDKVIQIWPEFDIDGAVELQYTFRLIQTGADGALIEQIVLT
                     DDMLDSPLEIPTKPFDAYISVTVKARGNGTVHLGPIHKRWSRLDMGQFLLGGSRFVDS
                     QRQEFIYYFHPGDMKPPLNVYFSGYRTAEGFEGYYMMKRMNAPFLLIGDPRVEGGSFY
                     IGSSEYEQGIINVIDETLEKLNFKSHELILSGLSMGSFGALYYGAQLNPQAIIVGKPL
                     VNIGTIAEHMRLLRPEEFGTALDVLVSNEGDTSQASIQALNQKFWQTFQKKSLSQTVF
                     AIAYMQHDDYDPNAFQELLPVLTAHQARVMNRSIPGRHNDDSPTIASWFVNFYNIILE
                     DKFGRVQHAEKQNI"
     gene            complement(22687..24240)
                     /gene="asp1"
                     /locus_tag="POB55_000365"
     CDS             complement(22687..24240)
                     /gene="asp1"
                     /locus_tag="POB55_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp1"
                     /protein_id="HDI6499361.1"
                     /translation="MKYFIPAWYDDQRWWQDTTVPYYQLQNKTEFDDMISLMGMHLEN
                     DLDYQLIVLNHAPNLRTFLHRYDLYETKYSSVFDEIQGFSHHAPQAINYHHLKWPDDV
                     EFVYTPYLLKCVTSEQTYTNIYFSQEGYSIWFEEFERDQLQRRYIFDDRGYLSAIRYF
                     DDQGEASYQEYLTINGDCVLHEDLKNGRVTVSKRYQHHYQQTEYNNMAQLIEEKFQAM
                     IAQQIHEDDHVIVASDARHNRQIANHIPAKSLSYSFFKNRNETVSDEEYQSIVKNAHL
                     IVDSVQLERDLISHQEKYQRENTMIRITPFETRQSPNISSQLMETFIGVWIDGMSDAD
                     LQQMMQRLVDYIAQEDYYRLILLSRHQNDIPMWLRECITSVNEEYQAKQNADVNVSAL
                     MTPEDQDDIIAVKTIHAEHDVVEALRTLRLVIDMSKEPDLYLQISAISAGIPQINGQQ
                     TDYVSDYDNGRIINTVDELDDALNYYLFYLKNWNYAYAYSLKLIDAYASKNIINQLDE
                     LIEGENDAT"
     gene            complement(24251..25462)
                     /gene="secY2"
                     /locus_tag="POB55_000366"
     CDS             complement(24251..25462)
                     /gene="secY2"
                     /locus_tag="POB55_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein translocase subunit
                     SecY2"
                     /protein_id="HDI6499362.1"
                     /translation="MLKLLQQYEYKIIYKRMLYTCFILFIYILGTNISIVSYNDMQVK
                     HESFFKIAISNMGGDVNTLNIFTLGLGPWLTSMIILMLISYRNMDKYMKQTSLEKHYK
                     ERILTLILSVIQSYFVIHEYVSKERVHQDNIYLTILILVTGTMLLVWLADKNSRYGIA
                     GPMPIVMVSIIKSMMHQKMEYIDASHIVIALLIILVIITLFILLFIELVEVRIPYIDL
                     MNVSATNMKSYLSWKVNPAGSITLMMSISAFVFLKSGIHFILSMFNKSISDDMPMLTF
                     DSPIGISVYLVIQMLLGYFLSRFLINTKQKSKDFLKSGNYFSGVKPGKDTERYLNYQA
                     RRVCWFGSALVTIIIGIPLYFTLFVPHLSTEIYFSVQLIVLVYISINIAETIRTYLYF
                     DKYKPFLSQYW"
     gene            complement(25592..32419)
                     /gene="sasA"
                     /locus_tag="POB55_000367"
     CDS             complement(25592..32419)
                     /gene="sasA"
                     /locus_tag="POB55_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-rich repeat glycoprotein adhesin SasA"
                     /protein_id="HDI6499363.1"
                     /translation="MSKRQKEFHDSLANEKTRVRLYKSGKNWVKSGIKEIEMFKIMGL
                     PFISHSLVSQDNQSISKKMTGYGLKTTAVIGGAFTVNMLHDQQAFAASDAPLTSELNT
                     QSETVGNQNSTTIEASTSTADSTSVTKNSSSVQTSNSDTVSSEKSEKVTSTTNSTSNQ
                     QEKLTSTSESTSSKNTTSSSDTKSVASTSSTEQPINTSTNQSTASNNTSQSTTPSSVN
                     LNKTSTTSTSTAPVKLRTFSRLAMSTFASAATTTAVTANTITVNKDNLKQYMTTSGNA
                     TYDQSTGIVTLTQDAYSQKGAITLGTRIDSNKSFHFSGKVNLGNKYEGNGNGGDGIGF
                     AFSPGVLGETGLNGAAVGIGGLSNAFGFKLDTYHNTSKPNSAAKANADPSNVAGGGAF
                     GAFVTTDSYGVATTYTSSSTADNAAKLKVQPTNNTFQDFDINYNGDTKVMTVTYAGQT
                     WTRNISDWIAKSGTTNFSLSMTASTGGATNLQQVQFGTFEYTESAVTQVRYVDVTTGK
                     DIIPPKTYSGNVDQVVTIDNQQSALTAKGYNYTSVDSSYASTYNDTNKTVKMTNAGQS
                     VTYYFTDVKAPTVTVGNQTIEVGKTMNPVVLTTTDNGTGTVTNTVTGLPSGLSYDSAT
                     NSIIGTPTKIGQSTVTVVSTDQANNKSTTTFTINVVDTTAPTVTPIGDQSSEVYSPIS
                     PIKIATQDNSGNAVTNTVTGLPSGLTFDSTNNTISGTPTNIGTSTITIVSTDASGNKT
                     TTTFKYEVTRNSMSDSVSTSGSTQQSQSVSTSKADSQSASTSTSGSIVVSTSASTSKS
                     TSVSLSDSVSASKSLSTSESNSVSSSTSTSLVNSQSVSSSMSDSASKSTSLSDSISNS
                     SSTEKSESLSTSTSDSLRTSTSLSDSLSMSTSGSLSKSQSLSTSTSESSSTSASLSDS
                     TSNAISTSESLSESASTSDSISISNSIANSQSASTSKSDSQSTSISLSTSDSKSMSTS
                     ESLSDSTSTSGSVSGSLSIAASQSVSTSTSDSMSTSEIVSDSISTSGSLSASDSKSMS
                     VSSSMSTSQSGSTSESLSDSQSTSDSDSKSLSLSTSQSGSTSTSTSTSASVRTSESQS
                     TSGSMSASQSDSMSISTSFSDSTSDSKSASTASSESISQSASTSTSGSVSTSTSLSTS
                     NSERTSTSMSDSTSLSTSESDSISESTSTSDSISEAISASESTFISLSESNSTSDSES
                     QSASAFLSESLSESTSESTSESVSSSTSESTSLSDSTSESGSTSTSLSNSTSGSASIS
                     TSTSISESTSTFKSESVSTSLSMSTSTSLSDSTSLSTSLSDSTSDSKSDSLSTSMSTS
                     DSISTSKSDSISTSTSLSGSTSESESDSTSSSESKSDSTSMSISMSQSTSGSTSTSTS
                     TSLSDSTSTSLSLSASMNQSGVDSNSASQSASNSTSTSTSESDSQSTSSYTSQSTSQS
                     ESTSTSTSLSDSTSISKSTSQSGSVSTSASLSGSESESDSQSISTSASESTSESASTS
                     LSDSTSTSNSGSASTSTSLSNSASASESDSSSTSLSDSTSASMQSSESDSQSTSASLS
                     DSLSTSTSNRMSTIASLSTSVSTSESGSTSESTSESDSTSTSLSDSQSTSRSTSASGS
                     ASTSTSTSDSRSTSASTSTSMRTSTSDSQSMSLSTSTSTSMSDSTSLSDSVSDSTSDS
                     TSASTSGSMSVSISLSDSTSTSTSASEVMSASISDSQSMSESVNDSESVSESNSESDS
                     KSMSGSTSVSDSGSLSVSTSLRKSESVSESSSLSGSQSMSDSVSTSDSSSLSVSTSLR
                     SSESVSESDSLSDSKSTSGSTSTSTSGSLSTSTSLSGSESVSESTSLSDSISMSDSTS
                     TSDSDSLSGSISLSGSTSLSTSDSLSDSKSLSSSQSMSGSESTSTSVSDSQSSSTSNS
                     QFDSMSISASESDSMSTSDSSSISGSNSTSTSLSTSDSMSGSVSVSTSTSLSDSISGS
                     TSLSDSSSTSTSTSLSDSMSQSQSTSTSASGSLSTSISTSMSMSASTSSSQSTSVSTS
                     LSTSDSISDSTSISISGSQSTVESESTSDSTSISDSESLSTSDSDSTSTSTSDSTSGS
                     TSTSISESLSTSGSGSTSVSDSTSMSESDSTSVSMSQSMSGSTYNSTSVSDSESVSTS
                     TSTSLSTSDSTSTSESLSTSMSGSQSISDSTSTSMSGSTSTSESNSMHPSDSMSMHHT
                     HSTSTSRLSSEATTSTSESQSTLSATSEVTKHNGTPAQSEKRLPDTGDSIKQNGLLGG
                     VMTLLVGLGLMKRKKKKDENDQDDSQA"
BASE COUNT        10025 a         6040 c         5120 g        11253 t
ORIGIN      
        1 ttttaaaaat gttatctgta actaaataaa attttaaaaa ttgagcttaa ttatatttta
       61 aaatttatca aggctttatt tttgtgaaaa tattaacaaa tacaaaatag tgctctatac
      121 acttaatcaa tgcttgaaat atgtgagata actatttaaa taatcatgat caaataaaca
      181 tttttatcaa tttcaaacaa ctatgtaaga cgcaataatc gttattattt aataaaaaag
      241 actagaatgc ggtatctccc ctcacactct agccttaata tgccattatt ttatttactt
      301 gttttgtatg gtgtatgtcc aactgttact tcgacaactc tattatgatc aacgacaaac
      361 cagatgccta atgtcttttt agcattttga tagtaaaaaa taccgctatc agcttccttt
      421 gttttaccat tttctttttt caagtccttg ccataagctt tttggatatc ttttaatgaa
      481 atattatttt taactacaaa ttgcaattga cttgcttcgt ttccttttgc agaaacacct
      541 ttaaaagttt gatcatattt ctctactttt tttatcgtat tcgttttagc taatttaata
      601 ccattgaatt tcacattatc atacttaatc gcgttaataa aattcttatc cagaataaaa
      661 ttagcattat taccaatata accattataa gtataatatg gggttattgc tgcctgtgcc
      721 tgtttacttg ttggcgccga attttcaaca actgttactc caatcagtgt gcccaatgct
      781 aatgttgctg ctacacaaac tttactggtt ttattcataa ttatctctct tttccataca
      841 aatattttat tccttacact tttattataa ctttttcagc cataaaaata ctaatgatta
      901 tatttataaa tacacaacag ttttgttata ctaattgaag tacacaaaaa acaatgaaaa
      961 tattaatatc gcctactttt ctaatattta ttttttaaaa tgattatggc aatattgaaa
     1021 taaattattt agcttcacgt atcaactact tttcattaac atggaggcgt tatatttgaa
     1081 aaaacttctt attaccatcg taattatcgt attaatcgga gcacttgggt tcgcaattta
     1141 tgctgcaata aatcattcta catcatcaag taataaccaa gaacaagaaa ataaagctac
     1201 gcataaaaag acagaaaccg aagaaaatga tcaacaagat gatagtgcag acaaagcaga
     1261 taaaaatcaa aataatggca ataacgttac taatgacaat caaccatctt cacagtcaaa
     1321 tgttcctaac aaccattcaa cagcaccatc gaatacgcaa ccagctcagc caaaagactc
     1381 aaattcaaat aaaaataata ctcacgatgg tggtagtcaa tcgtcaacaa acaaccaaaa
     1441 taatcaacaa cagaacacac cacctgctaa taaatcaaat aataccaata cgtcttctaa
     1501 acaaacacct caagcacaat cacctaaaaa ttaatcattt acctgatacg tccacaagta
     1561 tcaggtacat aaattcagaa gaacagcaat atgatatttt tctatttgaa atatcttact
     1621 gctgttcttt atttgcataa ttcgcactct gttaaattca tataaaagtt cgctttaatg
     1681 tcattgacgt atgtataagc tctcatgttt aataatatta atacaccttt aaaaacgctt
     1741 acacggaaaa tttagaaagg aggtacatgt taaatgcttg atagacactt aaaactactc
     1801 cagtttttta ttaaaaatcc atcaaagcac attagttcta atgaaattgc tgaacatgtc
     1861 aatgtttcaa accgcacagt tcgcaatgat attcatgtta tcaatagcaa ttttatggat
     1921 gatatcatcg ttagtattaa atctaaagga tatcaattaa atacatcgca gtatacctta
     1981 gaaacgataa ctgaacgtta cacgcatatt caatcatata aagaaaaact attattatct
     2041 atggcgtatc aactgctaat gcacaataaa tcgcagacat tgcaacaact agagcaagat
     2101 tatctattat ctaaaactgt gctcaacgac tatttcgttc gaattcaaca atggtgtcaa
     2161 aaatttaata tcgctttaac aatcaagaaa aaacaaggta ttgtggtcga tggaacagat
     2221 aatgatatca ctaatgccat catccattta aatcaattat catcagggca tgtacatgtt
     2281 gaagacctaa ttttaaatga attacctgat tcacaccaaa ggatgatttc gcatattata
     2341 caagagacac ttcaacaaca tagtattgaa acaactgata ttcaaataca acagctttta
     2401 attcatctca ttttaatcat taaacgtaag caatctttaa gtgagctaga cacgttaaac
     2461 cacgattcaa aattgatatc tcaatcttgc attaagcaaa tcaacgaaag attaggttat
     2521 gacttgaacg aaaaagtaat caatatgttt tcattcttta ttgcttatca ttttaataaa
     2581 ctcgatattg gcatagaacg tatatttata caaagttata ttgatcgctt aatcgaattg
     2641 atgcagcaac gtattcatat acctttcaat gaagattcga ttttacaaca aaatttatat
     2701 aatcatttta gtaaagcata tttacgtatc actcaaaaca tttatttaaa caatccttta
     2761 gttatagaga ttaaaaaact ctatcccttt gtgtttaaca cactttttga agcgattgat
     2821 aaattagcaa ttgatactga tatagagatg agcgaagatg aaattgcatt tttaacaatt
     2881 cattttcaag ctgccatcga gcgtcgtacc aaaacacaat taaatgtagt cattgcttgt
     2941 tattatggtc taggtgtgtc gaatttttta gaaactaaaa taaataactt atccgaagag
     3001 ttgtcagtga taaatacgat taaactagaa aatataacac actatcactt tgacaatgta
     3061 gatttattaa taacgaccca tgatattcca aaacaaacgc taaacatact tcctaagcac
     3121 ctaacgacta tcaaggttgc accattattt tctgaagatg accgtcacaa aatcagactc
     3181 gttgtgaagc aaaagcaaaa tccagttcaa gcacatcatc atatggacac tgtcaatttc
     3241 cttgtcgtaa atactgaaca aaaatcacgg catactgtgc aaatttttga agaagctcaa
     3301 aaaatattac aagcacatca tgcgattgta gaaggatata tcgaatcagc tttagagcgt
     3361 gagaaatcat cttctacata cataggtaat tttatggcga ttcctcatgg agatcctgaa
     3421 aaagttttac aatcacatgt gcttattttt cgcacaaaag atgtttttcc ttggcgacaa
     3481 cacgatgtca aacttgtctt tttcttagcg atttcgcata aagacaaagc ttttacaaaa
     3541 caaatgatgc aattaattgc taacttggat gatgattcag tgaaccattt atgtagttta
     3601 gatgatcata gtttaaagca acagttattt gaatacttac aagaataatt gccactagta
     3661 acggtaatta ttcttttttt tagtatcgag aatacgctta caatatagaa aaggagatga
     3721 ttttatgaaa attgtagcta ttacctcatg cccaaatggc attgcacata catatatggc
     3781 tcaagaaaag cttgagcagg tagcaaaaga aatgggtgtg gatattaaag tagagacaca
     3841 gggcggtgtt ggtgctgaaa atgttttaac tacgcaagac atcgaagaag ctgacggtgt
     3901 cataattgca gctgataaac aagttgatct gtcccgattt gttggtaaac ggttgattaa
     3961 tgaaaatgtt cgcgaaggga ttcataatcc gagaggtcta attcaacgta tcattaatca
     4021 agatgcgcct atttatcaat ctgaaacaaa ttatcattcg aaagatcgcg gtaagtctaa
     4081 aagtggtatt caaatggtgt atcaacattt aatgaacggt gtatcgttta tggttccttt
     4141 tatcgtagtt ggtggactcc ttatcgccat cgcgctgact ctaggcggtg aaacgacatc
     4201 aaaaggatta gtcatcccag atgattcatt ttggaaatcc attgaaaaca ttggtagttt
     4261 agcctttaaa tttatggttc caatactagc tggatatatc gctgttagta tcgctgacaa
     4321 gcccggtctt gttcctggta tgattggcgg cgctatcgct gctgacggta gtttttatgg
     4381 cagtgatgca ggcgcaggat ttttaggtgg tattgttgct ggtttcttag caggttatat
     4441 cgcaaagtgg attaaagata ttaaagttcc aaaagcaatg gcaccaatta tgcctatcat
     4501 tattattccc attatttcat cggtcgttgt aggattgata tttatatttt taatcggtgc
     4561 accaatttct aatatttttg aggcattaac aacctggtta aaaagtatgc aaggtgctaa
     4621 tattattata cttgcgatga ttataggtgc gatgattgcc ttcgatatgg gcggtccagt
     4681 aaacaaagta gcatttttat ttggctcagc acttattgct gaaggtaatt acgccgttat
     4741 gggtatggtc gcagttgctg tatgtacacc accaatcggt ttaggtttag caacgtttgt
     4801 tcaaaagtat aaattcaatc attccgaaag agaaatgggt aaggcttcct tcactatggg
     4861 actgtttggt attactgaag gtgctattcc tttcgcagcc caagatccat tgcgcattat
     4921 acctgcaaac atcattggtg caatgattgc atcagtcatt gcagccattg gtggtgtcgg
     4981 cgatagagtc gcacatggag gtccaatcgt ggctgtatta ggtggtattg atcatgtatt
     5041 atggtttatc tttgcagtga tagtaggtag tcttgtcaca atgactactg tactattatt
     5101 aaaacgcaat acaccagtta tagcagtaga tgcaccagcg caacacacgc aacttcatga
     5161 tacagatatt acacaacatg atacagaggt tgacaatgta gataaaaccg cgaaacattc
     5221 agatctcaat aaacattctc atgtttttga tcaacaaact atgataatta ctgatcacga
     5281 catgtctcga aacgaagcaa ttgatatgtt gattcataaa cttaaaattt gccgttatgt
     5341 agagcacaca tcgcatttaa aaaatgcaat cctagaacgt gaaatggagt ctacgacagc
     5401 tattggcatg aacgtggcca ttccacatgc aaagtctgat gttgtaaaac aaccgattgt
     5461 agctgttatg aaaaataatc atggtgttaa atgggacagt ctagatggta gtctacctca
     5521 attgatattc ttaattgccg taccaaacaa cagtcaggat acacatttaa aaatacttca
     5581 gcgcttatct aaagcgttaa tgaatgatga gacacgccaa tcactaatta atgcaaacag
     5641 tactacagaa atttataact tattaatgaa aatatagagg agttttaact atggcattat
     5701 ttttacaacc cgtttttaaa gacagaattt ggggcggtca tgcattaaaa gcatttaact
     5761 atgatatccc taatgagaca actggtgaat gctgggcaat ttctgcacat ccaaatggtc
     5821 ctaatacaat tatcaatggt ccatacaagg atatgaccct agatcaactt tggtcacaac
     5881 atcgtgaatt attcgataat gattcacgag atagtttccc tctgctaact aaagtattag
     5941 atgccaatga taaattatct gttcaagttc atccagatga tgactacgct ttaaaacacg
     6001 aaggcgaact aggtaaaaca gaatgttggt atattttaga tgcagcccca ggtgctgaaa
     6061 ttatatatgg cgtacatgca cataataaac agacgttaat agacatgatt gataatcatg
     6121 aatttgacag tcttttcaaa cgtatacctg ttaagcccgg tgacttttat tatgtgcctg
     6181 ctggcactgt ccatgccatt ggttcgggta ttttaatttt agagacacaa caatcatccg
     6241 atacgacata ccgtatttat gactatgata gacgagatca aaatggtcaa ttgcgtgatt
     6301 tgcacttaga gcaaagtaaa gatgtgattg aattaggcaa tcatgaacca aatactactc
     6361 ctataacaac tcacatcgac acacacactg taactcaatt tgtagataat caatattttg
     6421 cagtatataa atgggatatt catggagatt taaaattcaa taaaccacat gcttattgct
     6481 tagttactat tatcgatggt actggtactt taacagtaaa cgataagcac tatgacatcc
     6541 aaaaaggttc tagctttatt ttaactacgg aagacgagga tattcacttt gtcggtaata
     6601 ttagtgctat agtaagtcat cctggataat agcaatgata acacccgtcc cataaaagac
     6661 agaaattact atctcttctg tctaaaaaca taaaaatcca cacattaagg agctataacc
     6721 ctaatgtgtg gatttaattt ttattctgta acgttacttt ggtcaacttt ttcagatacg
     6781 cgcttcatca atggatctgt tacaggtttt aaaattaaac ctacaacgaa gaatcctatg
     6841 ccaaataacg ttaatataat taattttgta attaggattt ccggaacaat accgcctact
     6901 gtttcacgta atgaatcaat tgcatacgta aatggtaagt atggcgaaat gttttggaaa
     6961 aattgtggcg tagtttgaat tgggaatgtt cctccaccac ctgcaatttg taatacaagc
     7021 aatacgattg caatggcttt acctggatta ccaagtaatg atacgcacgt atatacgatt
     7081 gagttgaaaa taatcgaaca gaaaatcgtt ataagtacaa ataatacagg tgactcaact
     7141 cctgctttta ggattaacaa atctccaacc gatacaatga gtgcttgcaa cataccaagc
     7201 attataaaga atcctgcctt acctaagaac acttgtcttg tcgttaagac tgactctaga
     7261 ctcttatgtt tattatcaac cgttaataaa cttaccatca agagtgcacc tacccatact
     7321 gacagtgcag tatagaatgg tgtcatacct gaaccataat ccttaactgg gaaaacatca
     7381 acagtcttct tattaatagg atttgcaata atacccgctt gctttttcaa atcattctta
     7441 agtgtgtcga ttaatttgtc gactgcatca tctttgtcta atttcttgaa aattttattc
     7501 gcttttttca aatctttttc aacatcaggc aatttatttt gagaaaaatc agctaaatct
     7561 gataaagcct ttttcgcttg tggctgattt ttatttagta atcctaccgc ttgatcataa
     7621 ccatttaata acgttggtaa atttttattc acacttgctg tcgcattagt taatcgctgt
     7681 tccaactgtg gtaagtcatt gcgcacaaag ttagcagctt tagctacatc acttttcact
     7741 ttcgggaagt ttgcttgagc atttttaaca gcactaatgt attgttgttc aatacttggt
     7801 aatactgttt gtaagcgatc aatacgtgtt ttaccatttt ttaaaatagc ttgtccactg
     7861 ttaatgactt gatcaattgt atttaattta gactgaacat tggataatgc agtttgccct
     7921 ttgttcaatt catccataat acgttgtgat attgatacta aagagctgtt aagatctttt
     7981 ttaacataat ctctaaatga tgataatgat gaatctagtt ttgaaagttg atctagtaat
     8041 ttagtagctt cagcagttcc tgaactacca ttctttaatg cattgcttaa ttgattaact
     8101 aaccttaatg attcattaat tcgattatta gctgctttta ccttatcaat ttcttttgat
     8161 aaatcaacat tttcattctt ctcaatctct tttaatgtat cgataaattg ttgattatat
     8221 tgcgttgtgt attctaaacc ggacttaaca ttatctaaag actctctaaa atcagaaggc
     8281 ttgtccgatg ctaaaatgcc atacgaaata ttacgtaatg cctgactatc tttttgtgcg
     8341 gctttcgctt gggtatctaa attatttgat aatgataata aagagcccgt taatgccgct
     8401 tccattgatt taacatcttt atctgtaatg ttttgtgtgt tctcgttagc tgtgctgact
     8461 tgtgttgttg ttatatctgc aaaactttga ccatcttttt tcccgcttgt actttgtggt
     8521 gtagtcgata caggcgcatt attactattt ggtgcggtat tttgcgtaac attattattt
     8581 ggtgcagtat cgccacttgg tgcacttggt ggcgttgatg ctacaccatt accagctgca
     8641 ggtgcactat ttgatgtttc gttgttcgcc gcgctatttt gaggaatctg ttgctggttt
     8701 cttcgattta aatcttgcgc gcgtcgaatt gcatcttgat aatcaccgac tctacttcta
     8761 acttgtgtga cgaagccttt ggcatcattt aactgtccat tagcttgatc aatcccttga
     8821 ctcgcaatgt taagtccttg attaatttta gggaactgcg ctggcacgtc atcagctgca
     8881 aaatttaacg ctttttcaat tttcggcata taattattta aagctaatat caacttagcc
     8941 ttttcattaa gttgcggaat agcaccattg acttcgttta attttttctg agcatctaaa
     9001 atatcacctt tataatttcc tagttttcta aaatcattgg catatttatc taaatccgct
     9061 tggtggttat tcaaatatac aattttattc gcaaagtcat taatcttagg taaagcttta
     9121 tctgccgaat ataccgcatt ttttatctta ttaattgtcg gcacattttc ttcaatagtt
     9181 aaacctgctt tgttagcttc ttctaataat gctcgagtta ctgttttatt aaattgttca
     9241 ttcgcttttt caacgacaac tgacgaacca gtatctgtta gcttagacgc aacagcgtta
     9301 atcttctgat tcaccttaaa ttctacatct gctttttgag gctgcttacg tagtgtcccg
     9361 gtaatttcat gtgtaaactt agatgggatg taaatacctg caaaatattt acccattttt
     9421 atctcatgat cagctttctc tctacttaca aactgccaat caaaactttt atttttcttg
     9481 agtgtattaa ccatcgtatt accgacatta acttttttcc ctctgattat gtcgccttta
     9541 tcttcattaa cgactgcgac cttgatgtgt cccgtgttgc catatggatc ccacattgcc
     9601 cataagttaa accaagcgta gaacgatggc aaaatagcta agcctgctaa gataatccac
     9661 acagctggcg tcttagctac tttcttcaga tccattttaa ataatttaaa tgcgttcttc
     9721 attgtcacac tcctatgtag gaattattca tattttttat atattttttg taaattaatt
     9781 tatttttgcg ttgtgaatta gtataatcaa tttactggaa gatatttagt cgattgatac
     9841 ctatcaacta ttttcagcat acgataaatt ataacaaatc atagtttatt atcacactta
     9901 attattatat ttttcaaggg agaatacgaa atatgcctaa aaataaaatt ttaatttatt
     9961 tgctatcaac tacgctcgta ttacctactt tagtttcacc taccgcttat gctgatacac
    10021 ctcaaaaaga tactacagct aagacaacat ctcatgattc aaaaaaatct aatgacgatg
    10081 aaacttctaa ggatactaca agtaaagata ttgataaagc agacaacaat aatacaagta
    10141 accaagacaa taacgacaaa aaatttaaaa ctatagacga cagcacttca gactctaaca
    10201 atatcattga ttttatttat aagaatttac cacaaaccaa tataaaccaa ttgctaacca
    10261 aaaataaata cgatgataat tactcattaa caactttaat ccaaaaccta ttcaatttaa
    10321 attcggatat ttctgattac gaacaacctc gtaatggcga aaagtcaaca aatgattcga
    10381 ataaaaacag tgacaatagc atcaaaaatg acactgatac gcaatcatct aaacaagata
    10441 aagcagacaa tcaaaaagca cctaaatcaa acaatacaaa accaagtaca tctaataagc
    10501 aaccaaattc gccaaagcca acacaaccta atcaatcaaa tagtcaacca gcaagtgacg
    10561 ataaagcaaa tcaaaaatct tcatcgaaag ataatcaatc aatgtcagat tcggctttag
    10621 actctatttt ggatcaatac agtgaagatg caaagaaaac acaaaaagat tatgcatctc
    10681 aatctaaaaa agacaaaaat gaaaaatcta atacaaagaa tccacagtta ccaacacaag
    10741 atgaattgaa acataaatct aaacctgctc aatcattcaa taacgatgtt aatcaaaagg
    10801 atacacgtgc aacatcatta ttcgaaacag atcctagtat atctaacaat gatgatagcg
    10861 gacaatttaa cgttgttgac tcaaaagata cacgtcaatt tgtcaaatca attgctaaag
    10921 atgcacatcg cattggtcaa gataacgata tttatgcgtc tgtcatgatt gcccaagcaa
    10981 tcttagaatc tgactcaggt cgtagtgctt tagctaagtc accaaaccat aatttattcg
    11041 gtatcaaagg tgcttttgaa gggaattctg ttccttttaa cacattagaa gctgatggta
    11101 ataaattgta tagtattaat gctggattcc gaaaatatcc aagcacgaaa gaatcactaa
    11161 aagattactc tgaccttatt aaaaatggta ttgatggcaa tcgaacaatt tataaaccaa
    11221 catggaaatc ggaagccgat tcttataaag atgcaacatc acacttatct aaaacatatg
    11281 ctacagatcc aaactatgct aagaaattaa acagtattat taaacactat caattaactc
    11341 agtttgacga tgaacgcatg ccagatttag ataaatatga acgttctatc aaggattatg
    11401 atgattcatc agatgaattc aaacctttcc gtgaggtatc tgatagtatg ccatatccac
    11461 atggtcaatg tacttggtac gtatataacc gtatgaaaca atttggtaca tctatctcag
    11521 gtgatttagg tgatgcacat aattggaata atcgagctca ataccgtgat tatcaagtaa
    11581 gtcatacacc aaaacgtcat gctgctgttg tatttgaggc tggacaattt ggtgcagatc
    11641 aacattacgg tcatgtagca tttgttgaaa aagttaacag tgatggttct atcgttattt
    11701 cagaatccaa tgttaaagga ttaggtatca tttctcatag aactatcaat gcagctgccg
    11761 ctgaagaatt atcatatatt acaggtaaat aagtattatt aaacccgtaa aatttataag
    11821 tataaacaag gagttcggac ttaaacatat ttctgttcat aagtccgatt tcttattcaa
    11881 ttaaacccga ggcattcagt tcgaacgcct cgggtcattt tatataaata tattatttta
    11941 tgttcaaatg ttcctcatca tatccgtttc aattgccatc tcacacattt tataaatatg
    12001 agcaaatgta cttattctca aacattactg ccgagcttta attgacgtta tattaactat
    12061 aaactacttt tccatgactc tacggattca atgtcacatg agcgtgataa aatttgttca
    12121 ataataaagt catgtttatc atctgatcta tcaccaacag catcttctaa aacagtaata
    12181 taatagtctt tatctacact ttctaatgcc gtgctcaata cagctccact tgtagagaca
    12241 cccgttaata ctaaatgatt aatatcattt gcacgtaaat aaacttccaa gtaactacct
    12301 gtaaatgcgc taaagcgtcg cttagaaata atcggctcat cttctagtgg tgctaaatct
    12361 tcaagtattc gtgtagatgc atctgcttca gtaatcgcat atccttgagc tttaattgtt
    12421 gaaaacactt tattactcga ggagacatca ttaaaatgct tatctaacac taaacgtatg
    12481 aaaatgactg gtattcgatg ttgtcttgct gcttcaattg ctctctgatt cgctttaata
    12541 atatttttta ttctaggtac actactcgct ataccttctt gcatatccaa aactaatagc
    12601 gccgtttttc gagacatctt cattctcctt tacttgtgta gttctaagtc gttaaattca
    12661 ttataacgtt aaaatgatgg acaatctatt cattgcattt tgcatatact tcacaataat
    12721 ttaaggggaa ataagacgtc ttatatactt aaaaaaaata tatagatgct cttcccccaa
    12781 tataattatg ctttattttt caacttattg cgtcgtgata accaaatcat tagtacaccc
    12841 attgcaccaa caattacaga tatcggcaac caatgttctt ttatcgtttc ccccgcttta
    12901 ggcaaagata cattaccatc agcatttaat aatccactta acaatccatt acctttacca
    12961 agtgttaacg tcttttctgg ctttggtgtg ggtatatctg gaatactgtc taataaattt
    13021 gatccttgat tcattaaatt tgctaactta tttaaatccg tcgttttccc atttttattc
    13081 aatcgatcta gtaaacttgg acgatttact attggtgata aaatatagtc tatatctttt
    13141 ttcgtttgat tgagtctctt ttgtaaattc aataaatcat ccgctttacc attcaatgcc
    13201 gatttaacta aattaaaaat tttattttga tctgtttcta ttttagtaat taaatctgcc
    13261 agtaattttg ccttttgtct ttctatacgt gttgctaaaa tcgtttcaat tgcttgtttt
    13321 ttatctttcg cattattcaa aattgctttt aatatatcat ctgaagacgt gtcgccagtt
    13381 gatgcaaaat gtttcttcaa ttggtcaacg atttggcgat ttgataatcc tttattcgtc
    13441 caatctttag ccaatttatc tgcttcagct tttcctaatt tcgtttgtaa aatttgagaa
    13501 atcaatagcg acttatcttg tgattgatca atcaatgacg ttaataaatc atcactcgtt
    13561 gtcagagata gttgatcaat atgacgagta atttgatctg caatttgttg atccgtttta
    13621 ccatcaacac gtatatcttt tagaatttta tctgcctcgt ctttattaaa tatactttct
    13681 aaaatgcttt gtgtagcata ctttttatca tcagtacgtg caagttcttc caaaataata
    13741 tttcgttgac tttttatacg ctctttcgtc ttatttactt cgctcattaa gtctgatttt
    13801 tgatttttag gaagttgcgt atttgcaata cgttgatcta aagattgtaa cgtattcagt
    13861 ttatgatatg tgtaatgttg cgttgaggca ttacttttag ccaatttttc aatcatagca
    13921 tgattaattt tatcgcttcc ttgtaattta tcagtgagtc gattactatg gctttgattc
    13981 tcttcatttg aaaggaattt atttaacaca acatgtccag aaccatcatt atttggcgtt
    14041 ttagctactt catgattact atctgttgta gacactgctg gatctttcga tgcatatttc
    14101 aatgcatctt tcgatttgtg tatttgctga ttcaaatggt ctaggtcttc taacgcctta
    14161 tttaccattg cttcatcatt tttatcatct ttttctccat gttttgttgt agccgtttgt
    14221 gacatatcat ttttcattgc attaagatcg tcctcgccac tttgttgacc cctatcaaca
    14281 tttgaagaaa cctcatttaa atctttaagc aattgatcta atttactgtc tatatcactt
    14341 tgaccgttca tttcagtgtg agaactttta ttgtctttgc tatccaactc attagctcgt
    14401 tttatgattt catctatttg cgatgctgtt ttcgcttcat ttagttgtgc tttataatgt
    14461 gctttagatg aagccgataa ctgttttaat tgctcaattt gacgaattgc tttgtcaact
    14521 ttgtctaata aatcttgctt agataatatc tcttttgaaa tttcagtatc cttttcagat
    14581 gcagcttggg catcgtacgg caagatattc gttaaaatga tacttgtcgc catcattgtc
    14641 gaacacgata actttacata taattgaaac ggttcccctc gatatttagc catcaatata
    14701 ctccttcctc acttacttcc ttcaaagaat tacatactat tatatacctg ttcacaagaa
    14761 atttatactt atctatctag ttattgttgt tagtaattat ctacttatta cttagcttat
    14821 atttaagtaa acaaaacaag catgacgtaa tatcatattg tccatgtcgc ttacatcata
    14881 ttacgtcaaa tcttttatat taaatgatgc tttattttag actgcttttt ctttttagct
    14941 ttcgagcgcc tgtttaaaaa cttgctcgaa ttgttcacgc gagatttcgt gtgcatgtgc
    15001 tttttgtgct aataaagcat cttgaaactg ttgttgatct ttcaaactct ctaacatttg
    15061 tattaattgg tctttacttt ccattgttat ctcatcatta tgctcaaata agtgctctga
    15121 taatgttact ttagcatggt gtgcggtttg acgataacct aaaatcaaca actcatagtc
    15181 aaacgcttgt tccaccgcat ttaaaatttc attaccctca ttgatatcaa gataaatatc
    15241 acataactgg tatagttcat ttaccctatc tatattaata gatgggtata aatgcacatt
    15301 agcatattga tcaagttgca ttagcttatc agacatctct gtaatagcag cgatgtgaaa
    15361 cttaaaatct ggtaaagttt caaccaatac cttgatgtta cgaagttgat ccgagttagt
    15421 taatattaca atttctttag tatatctatt acgactacga tagttatata gatatccgcc
    15481 ttgtaaaata cgagattgaa cctttgcgtc tgctatattg agcatcgttt catattcgtt
    15541 tttatctgga ataataatat tacaatgtcg tttcatatca cctttacaca tcaattgcat
    15601 atttcccggg acattaccat tacagtgttc ttgccatacc aaaacatcac taccttttga
    15661 tggcaaatta tataacactg aaaatggtag ggctagtgag ttaataacga aatgatgttc
    15721 cgtaatttca agttgcttga taaaaaataa tacgaatgcg agctttgaag ggaaaaagta
    15781 agacttccct tgccaatcca atatgacatc agatgttaca aaattttcat aaatcacttc
    15841 tttaccttct gctgtcatat atttcttcaa gatcgcttta cgatttaaat cgtaaacagt
    15901 ttgtgcaaat ttaataccat tcttagaata ataatcgaca aatcggacac gttgttggtc
    15961 atcaaaccat tcgacacgac taacaattct agggcgctct ccactttgat aaaatatttt
    16021 gcctcgtaga cgtcccatat cattaattgt agccgaattg ttgttacctt taatttccca
    16081 aaaagctggt acagtaacct gattaaaaaa tcgtggtttc atattttctg tattatgatt
    16141 atctgcaaaa aattgatacg gtgatataac atcgtccggt aaaaagccat tgtcattgag
    16201 tacaattgtt aaatcttctt ccaacttact ggctttaaaa gactcatata actttcgtga
    16261 atgattgtta aagtaatcaa ataatttaat catgtagcac ctcttgaact aatgtttccc
    16321 attttaaaat aatatcttga gtcataaatt gctgtgccac ttcataagag atgtcatgtg
    16381 gtgtctgggg accattgtta aaatacatta caatggcatg agctagtttt gcgataacat
    16441 catccacact atcttcatcg gtatcaaaag gtaccaagta gccattttcc ccatctcgaa
    16501 taaaggttgg gttaccataa ttcacattta atccaatcat acctagtcct gagcctaccg
    16561 cttccattag tgttaaccca aaaccttcgc tagttgatgc agaaagaaat aactcataat
    16621 cattataaat ttcatcaagt ttaacatgcc ctagtaaacg aatataatct tgcgcgcggt
    16681 gtgtatcaat aattttacgc agtcgcgtct tctcgctacc ttctccataa atatcaaatg
    16741 ttaattctgg cacttgtcgt ttagccacga taaccgcttt gacaagccaa tcaatatgtt
    16801 tctcatttgc taaacgagat gcactaatca tcgcatatgg ctttcttgat aatttaggat
    16861 atgataatgc atcaatgctt cccacaggaa tagtataaac acgtgggcga taaccttgat
    16921 attgctcaaa ttgtcgacaa accatatgat tttgaatatc tgttgctgta ataaagaaat
    16981 caatgtattt agcttttgaa aattgatatt cataataatt gttccatagt atatgctgct
    17041 cactcatcat attattacta taatgatcag catgaatcac aacaccaact ttactatcac
    17101 ctttatgctg caaaacagcc tgaccaatat cagaagcgcg gtctaatatg acaatatcgt
    17161 ctcgggttaa attcaatcgt tgtaaaaagt atgcaataaa ttcggttttg ttatacaaca
    17221 ccgcatcttc aaacacatat atagagctgt ctccatcaat atattcgtta taagcgatgg
    17281 aaccatcttc attatagaat tgtcgcatat ataatttcgc tttattatca gctggtgcat
    17341 aatactcaga aaatatacgc gtataactat aaaaatcttt acgtactaac atactattaa
    17401 ttacaaattc tgcacgatcc acaacatctt tttgttcatt ttgtagataa catgttacaa
    17461 atgatgattt cccattaaaa tatagacgga ctatcttacc atttctttct ctaaaactaa
    17521 tttcatgacc aagctcacgt tcaatgtcat ctaacgtgta cgttgttggt gctatagaaa
    17581 tatcactaaa atactgatac aaccaaataa cttcttgatc tttaaaccca atgttttgcg
    17641 ttaatgtctg tatgttctct gactgtataa aatctaaaaa cacaaattta gtgtcttgat
    17701 ttgtacgtct caataattta gcacggtaag cttgtgcata ttcaacaccg ctactcgccc
    17761 agcctatacc aaagtttata ttatatattg tcatgcgcta ccccttttca tttatggaaa
    17821 atgtataact ggcataccct ctttatcaaa tgtaatcatg ctttgacaaa tatttttcac
    17881 cattcttttt ttgatatttc gtgtcataac ttcaaatgaa tctaatgcaa ctctatggta
    17941 ttcaaaaata gcgttacgtt gcccattttg tcgttgatta acgcttgctt ttaattgttg
    18001 taaatagtcg acttgttcta accaacatga atcaattgct ttcaaaaaga ctttttgaac
    18061 gaaaatatta taataatatg cactttgcat gtttttacga ttcaaagcta attgcttttc
    18121 aaattgctct aataaaaatg tcactactgc ttgcttatct ttaaaattaa cacaagccac
    18181 atctttatta aattggaaac ttaaattttg ataaatatac tcgacaacac gcgattttgt
    18241 tagcaccttt tcctcattta caaacatttc aaatacatct ttagctaacg ctttaaaatc
    18301 ttgattctca gcatcatcta tttctaaaac tcgattgcgt tcctcgtata caagatctcg
    18361 ctgtatacta atgctttttt caaattcatt agccatttca cgagctttaa ccccttgttc
    18421 ttccgagata cgctgcgctt taactacaat ttgcttaact ttgcgattaa acaaattact
    18481 ttgcgataat cgttgtgcat ctaatgaata taattgatta ttttccgcta aattactatc
    18541 gctccatcgc ttaactaaat aatcatctag tgaaatatat atacaagatg atcccggatc
    18601 cccttgtcta ccagaacgac cacgtaattg cctgtctaca cggctatttt ccatatgttc
    18661 atgaataata acagctaatc cacctaatgc ttcgacacct tcaccaagtt taatatctgt
    18721 gcctcgacct gccatactag tcgcaacagt catggaacca atttgccctg cttcagctat
    18781 catctgcgct tcttttgcaa cattttgcgc aatgagtaaa ttattaggaa tatccatttg
    18841 gaataatact ttcgaaaagt attcagccgc ttcagcagtt ctcgttatga gtaaaaccgg
    18901 tcgccccgtt tcatgaagtt caactatatc atgaatcatc gcgatgtttt tctcatcaac
    18961 tgaacgaaac actttatctg gttcatcgat acgttgaatc gctttatcag ttggtacttg
    19021 tacgactatt tttgaataca aatcaaagaa ctctgattcg cctaattttc ctgtagctgt
    19081 catacctgaa aatgattcaa aaagtttaaa taaattctgg aaggtaattg ttgccataac
    19141 acttttatct gttgaaacct ccataccttc tttcgcttca atagcttggt gaagtccagc
    19201 ttgcaactta gttcccggta acatacgacc tgtaatacgg tcaattaaaa caatatcacc
    19261 attatataca aagtaatcga cattagattc aaacaaatat tgcgcgcgca gtgctagatt
    19321 aatattacgc actaggacca tcgcttgttc gctatataaa tcttcaacat taaagtatga
    19381 ttgtgccgct tcaatacctt gatttaacag ccatatttct tttttggtct tcttcatttt
    19441 aaaatgcacg tcttcaatca atgtatctac aaactctttc acaatatgaa atagatttga
    19501 ttgtaatctt ggtgcgcccg aaataactaa tggtgtttgc gcagcatcta aaatgattga
    19561 gtccacttca tcaataatac cgtaatttaa ttgtggtaaa aatttccctt ccgcactatc
    19621 agccaaatta tcaattaaat aatcaaaacc gagacgtcca ttagttgtat atataatatc
    19681 atgttcatat aaattacgtt tttccccttt ttgatactca taatccacaa tatcaacaaa
    19741 acctaatgaa gcagttaaac ctaaccattc atataatggt tgcatctctt caaaatcacg
    19801 cttcgctaaa taatcattcg ttgtaattaa atatgttcct tttcccgaaa gtgcatttaa
    19861 atataaaggc atcgttgctg ttaatgtttt accttcgcct gtttgcatct ccgcaatgtt
    19921 accttcatgc aatacaatcg ctccgattaa ctgaacttct ttaggataca tacctaatac
    19981 tctccagctc gcttcacgtg ccactgcata agcttcaggt aacaatgtat ctagtgtatc
    20041 aactcctgat gctaaacgtt ctttaaattc tattgtcttt tgttttaacg catcatcaga
    20101 atatgattta acttcatcgc cccatgtatt gatgcgtttc actatttttc taatcgactt
    20161 tagtcttaat tcgtttatcg taacatctag tttatgtttc atttacttcc ccaccattca
    20221 gtttcgatac atctaagtaa tctaaaaatc gtactggatt cattaaacgt gacatataat
    20281 ttagatggtt gtcttgctct tctttaaaat aaacctcgac atttgtatct tttagttcat
    20341 gatttcctgg gacatgttct ttaagccatt cttttaaatc atcatcttca tggcttgtac
    20401 gatatacttt gcaatctaaa tgctgagcga cataagttgc aaaaacattt gactttgacc
    20461 cataactaat caaattaata gccgttaggg tatcttgact ttgcaaatca tcctttagtt
    20521 gcttaatatt ttccttgata tcgtcttcca tccaacgttc gacgagccaa acatgaccaa
    20581 acagtttcaa aaaatcattc gaaatagttg gatagacgtc agatggttct gcaataatga
    20641 catttatttt atcatttcca tattggttat cgtctatctt cgttacccgc atgcttttat
    20701 attctaaatc atcttgatgc gtcatctctg tgattgttaa acatctaaat acaagactcg
    20761 tcgatgctgc attcatcatt tttattttat aagcataggc ttcatcagga tattgaatcg
    20821 taatactatt tgactttaca atctcagtac ttagttttgt gccattttta ttataaaaaa
    20881 tgatgataaa atacactgaa ccagcaggcg ttgcatcaaa atcaaaatgc aatttataat
    20941 gctgtcctct acgcaaaatt ggtaaacttg gcgcactttt atattttgaa aattgcttta
    21001 acatcaacca ctcatgaatc ggtaatccag agggcatcaa agggtttata aaagtcactt
    21061 caccatttga aaatgatact ttagagccat acataaatgt agtttgtgaa atataattcc
    21121 aagtaacttt aaatgttttg tttttcagca tgttgaactc tcccaaactt gtcttccaaa
    21181 ataatgttgt aaaaattaac aaaccaactt gcaatggtag gtgaatcatc attatgtcgc
    21241 ccaggaatac tgcgattcat cactcttgct tggtgtgctg tcaatacagg taatagctct
    21301 tgaaatgcat ttggatcata atcatcatgt tgcatatatg ctatggcaaa aacagtttgt
    21361 gacaatgatt tcttttgaaa tgtttgccaa aatttttgat ttaatgcctg tatcgacgct
    21421 tgagatgtat caccttcatt agacaccagg acgtctaatg ctgtaccgaa ctcttctggt
    21481 ctaagtaatc gcatatgttc agcaatcgtt ccaatattaa caagtggttt accaacaata
    21541 attgcctgag gatttaactg agcaccataa tacaaggcac caaatgaacc catagataaa
    21601 ccagataata ttaattcatg tgatttaaaa ttcagctttt ctaatgtctc gtcaataaca
    21661 ttgataatac cttgttcata ttcagatgaa ccgatataaa aactaccacc ttcaacacga
    21721 ggatcgccga taagtaaaaa cggtgcattc atacgtttca tcatataata tccttcgaaa
    21781 ccttccgctg ttcgataacc actaaaatat acgtttagtg gtggtttcat atcaccaggg
    21841 tggaaataat aaataaattc ctgtcgttga ctatctacga aacgactacc accgagtaaa
    21901 aattgaccca tatctaatct agaccatcgt ttgtgtatag gtcctaaatg taccgttccg
    21961 ttgccacgcg ccttaacagt tacacttata taagcatcaa atggtttcgt aggtatctct
    22021 aaaggactgt ctaacatatc atcagtcaat acgatttgtt caattaatgc accatcagcg
    22081 ccagtctgaa tcaatctaaa tgtatattgc aactcgaccg caccatcaat atcaaattct
    22141 ggccatattt gaatgacttt atctttatcg taaacgagat tattttgcca agatgcgata
    22201 ggtttaaatt cttttccaaa ttctccactc aatgtgagct ctgaattacc ttggtaaacg
    22261 acatctcctt taaaattcgg atgcacaagt gctaacttag gagaaacctt atctccatac
    22321 tgtcctgaga agctaactgc ctctaattta ttattacgtt cttcaatatt ccggtaatgt
    22381 aatggttgaa caacgtattt ttggacattt tcgtcttgtt catattcaac tgaccaaaat
    22441 gattcatcaa catacgtatt gtatggttca cttatcattt gtaataaatt cgttaatgtc
    22501 tccgagtatg gtgcttgaat atagataaaa tcaaagcgcc cttctgcttc aacaatcgct
    22561 tcaatagcct ctacataacc actatcaaat tcaaacaatc caatatcgaa gtaatcccaa
    22621 ctcacacctt ttttgtgttg aaaaataggt tctaaatcgt ctcctccaat ttgcaaaact
    22681 ctaaatttac gtggcatcat tttcaccttc tattaactca tcgagctgat taataatatt
    22741 cttagaagca tatgcatcta ttaattttaa agaataggcg tacgcataat tccaattttt
    22801 caaataaaat aaataataat ttaacgcatc atctaattca tcaactgtat ttataatacg
    22861 gccattgtca taatcagaga cgtaatctgt ttgttgacca ttaatttgtg gaatcccagc
    22921 gctaattgca ctaatttgta aatacaagtc aggttctttt gacatatcta tcacaagtcg
    22981 caacgtccgc aatgcttcta caacatcatg ttcagcatgt atcgtcttaa cagcaatgat
    23041 gtcatcttga tcttcaggtg tcattaatgc tgaaacatta acatccgcat tctgtttagc
    23101 ttggtattcc tcatttaccg acgtaataca ttcacgaagc cacatcggta tgtcattttg
    23161 atggcgcgat aataaaatta aacggtaata atcttcctgt gcgatataat ccacaagtcg
    23221 ttgcatcatt tgttgcaaat cagcgtcact cataccatct atccatacac ctataaatgt
    23281 ttccatcaat tgactactta tattaggtga ttgtctcgtt tcaaatggtg tgattcgaat
    23341 cattgtattc tcccgctgat acttctcttg atgactaatt aaatcacgtt ctagttgcac
    23401 actatcaaca attaaatggg cattctttac gatagattga tattcctcat ctgacacagt
    23461 ttcatttcta tttttaaaaa atgaataact taatgatttc gctggaatat gattggctat
    23521 ttgtcgattg tgcctagcat ctgaagccac aatcacatga tcatcttcat gtatttgttg
    23581 tgcaatcatt gcttgaaatt tttcttcaat tagttgagcc atattgttat attctgtttg
    23641 ttgatagtga tgttgatatc tttttgaaac agtgactctg ccatttttca aatcttcatg
    23701 aagtacacaa tctccattaa tcgttaaata ttcttggtaa gaagcctctc cctgatcatc
    23761 aaaataacgt atcgctgata aataacctct gtcatcaaaa atataacgcc gttgtaactg
    23821 atctctttca aattcttcaa accaaattga atacccttct tgactaaaat aaatatttgt
    23881 ataggtctgt tcactcgtca cacattttaa taaatacggt gtgtacacaa actcaacatc
    23941 atccggccat tttaagtgat gataattaat cgcttgtggc gcatggtggc tgaatccttg
    24001 aatttcatca aacacagacg aatactttgt ctcatataag tcatatcgat gtaaaaatgt
    24061 tcttaaattt ggtgcatgat tgagaacaat cagttgataa tctaagtcat tttcaaggtg
    24121 cattcccatt aaactaatca tatcgtcaaa ttccgtctta ttttgtagtt gataatacgg
    24181 cacagtcgtg tcttgccacc atcgttggtc atcgtaccaa gctggaataa agtatttcat
    24241 aattacctcc ttaccaatac tggcttaaaa atggcttata tttatcaaaa tataaatatg
    24301 tacgaattgt ttctgcaata ttaatactga tgtaaactaa tacaattagt tgtactgaga
    24361 aataaatttc agtagataaa tgcggtacaa acaatgtgaa ataaagcggt ataccaataa
    24421 tgattgtaac taatgccgat ccaaaccaac atacgcgtcg tgcttgataa tttaaataac
    24481 gttctgtatc cttaccaggt ttaactcctg aaaaataatt gccactcttt aagaaatctt
    24541 tggatttttg tttagtattg attaaaaatc tcgataaaaa ataacccaat aacatttgaa
    24601 tcactaaata caccgaaata ccaattggac tatcaaatgt cagcattggc atgtcatctg
    24661 atatgctttt attaaacata gataaaataa aatgaatgcc actttttaag aaaacaaaag
    24721 ctgaaatgct catcattaaa gtaatactgc ctgcagggtt aactttccaa gataaataag
    24781 atttcatatt tgttgcggaa acgttcatta aatcgatata tggtattctc acttctacta
    24841 attcaataaa taataagata aacaatgtga ttatcacaag gatgattaac aacgcaatca
    24901 caatatgact tgcatctata tattccattt tttgatgcat cattgattta ataatgctaa
    24961 ccattacaat cggcattggt cctgcaatgc cgtaacgact atttttgtca gctaaccaaa
    25021 ctaataacat cgttccagta accaaaatca atattgttaa gtaaatattg tcttgatgaa
    25081 cacgttcttt cgaaacatat tcatgaatca caaaataact ttgaataaca cttaaaatta
    25141 atgttaagat gcgctcttta taatgcttct caagacttgt ttgtttcatg tatttatcca
    25201 tattccgata agaaatcagc atcaagataa tcattgatgt taaccacgga cctaatccta
    25261 aagtgaaaat gtttaaagta ttaacgtctc cacccatatt agaaatagct attttaaaaa
    25321 atgactcatg ttttacttgc atatcgttat aggaaacgat ggaaatgttt gtgcctaata
    25381 tataaataaa caagataaaa catgtgtata gcatacgttt atatataatt ttatattcgt
    25441 attgttgtaa aagttttaac atgttgcacc tcttttatat caaaaacatt aaaaagacta
    25501 agggttcatc actaattatt aaaatcctat atcgattttt ctagtgattg gtgcctcagt
    25561 ctttttaatt ttagccagct ataaattcaa tttatgcttg agaatcatct tgatcatttt
    25621 catctttctt tttctttctc ttcattaaac ctaaaccaac taataatgtc ataacgccac
    25681 ctagtaatcc attttgtttt attgagtcac ctgtatctgg caatcttttt tcactttgtg
    25741 ctggcgtgcc attatgttta gtcacttcag atgttgcact taatgtagac tgagattcac
    25801 tcgtgctcgt tgttgcttca cttgataagc gagatgtgct cgtgctgtga gtatgatgca
    25861 tactcattga gtctgacgga tgcattgagt tagattcaga tgtacttgtt gagccggaca
    25921 tacttgttga tgtcgagtca gaaatgcttt gtgaaccaga catagatgta ctcagtgatt
    25981 cggatgtgct tgtcgaatcg gatgtgctca atgacgttga tgtgcttgtt gacactgatt
    26041 ctgagtcgct cactgaagtc gagttgtacg ttgaaccgga catactttgt gacattgaaa
    26101 cacttgttga atcggattca ctcattgatg ttgagtcaga tacgctcgtt gaacctgaac
    26161 cagacgtact taatgattca gatatgcttg ttgaagttga accacttgtt gagtccgatg
    26221 tacttgtcga tgtcgagtct gaatctgatg tactcaatga ttctgagtca ctgatagaag
    26281 ttgaatcact tgtagattct gattctactg tactttgtga accactgata cttattgaag
    26341 tagaatcact gatactgtct gatgttgata atgatgtcga caccgatgtg ctttgtgatg
    26401 acgatgtact agcactcatt gacattgatg ttgatatcga tgtacttaag gaaccagatg
    26461 cacttgtact tgttgactgg ctttgtgaca ttgaatcact taatgatgta gatgtgcttg
    26521 ttgagctcga gtcacttaaa cttgttgaac ctgatattga gtcacttaaa cttgtcgatg
    26581 ttgaaactga tacgcttccg ctcattgagt cagatgttga aagtgatgta ctcgttgaat
    26641 ttgatccact gatgctagac gaatcacttg tagacattga gtcgctttct gatgcactga
    26701 tgctcataga gtcaaattga ctattacttg ttgagcttga ctgcgaatcg ctcacacttg
    26761 ttgacgttga ttctgatcca ctcatacttt gcgagctact caatgatttt gaatcactta
    26821 atgaatccga agtgctaaga cttgtggaac cacttaaaga tattgatcca cttaatgagt
    26881 cggagtcact tgtactagta gaatcactca ttgatattga atcacttagc gaggtagact
    26941 cgcttacgct ttctgaacca cttaatgatg ttgaggtact caatgaacca gatgtacttg
    27001 ttgaagtcga accacttgtc gattttgaat cacttaatga atcagattca ctcacgcttt
    27061 ccgaacttct tagtgacgtc gatacactta atgatgacga atcgcttgtg cttactgaat
    27121 cgctcatcga ttgtgagcca ctcaatgaac tagactcgct tacactttct gattttctta
    27181 atgacgttga gacgctcaat gagccagaat cacttacgct tgttgaacca ctcatcgatt
    27241 ttgagtcact ttcagaatta gattcgctta cactttctga atcatttaca gattctgaca
    27301 tactttgtga atcagatatg cttgcgctca ttacttcact agccgatgtt gatgtgcttg
    27361 tcgaatcact taacgatata gacacactca tcgaaccaga tgtactcgca cttgttgagt
    27421 ctgatgttga atcactaaca ctatcagata atgacgttga atcactcata cttgttgatg
    27481 tacttgttga aagcgacata ctttgtgaat cacttgtact tgtacgcatc gaagtactag
    27541 ttgaagctga tgtactacga gagtcacttg ttgatgttga tgtacttgct gatccagatg
    27601 cacttgtact tcttgatgtg ctttgtgaat cgcttaatga tgttgatgtt gaatcggatt
    27661 cacttgtact ttctgatgtt gagccagatt ctgatgtact taccgatgtt gataaacttg
    27721 caatggtcga catgcggttt gaagttgatg tacttagcga atcacttaat gatgctgatg
    27781 tgctttgtga atcggattca ctgctttgca ttgacgcaga tgttgaatca cttaaagatg
    27841 ttgacgacga atcggattca cttgcgcttg ctgagttact gagcgatgtt gacgtacttg
    27901 ctgatcctga gttacttgta cttgttgagt cactgagtga tgttgacgca ctttctgatg
    27961 ttgattcact tgcacttgtt gagatacttt gtgaatcaga ttcactctct gaaccactta
    28021 atgacgctga tgtgcttacc gaacctgatt gactcgtact tttagatata cttgttgaat
    28081 cgcttagtga cgttgatgtc gatgtggatt cactttggct tgttgactga cttgtatatg
    28141 atgatgtgct ttgtgaatcg gattcgctcg tgcttgtact tgttgagttt gaggcacttt
    28201 ggcttgctga gtttgagtct actccgcttt gattcattga tgcacttagt gacaatgacg
    28261 tactcgttga gtcagacaaa cttgtactcg ttgacgtact tgtacttcct gatgttgatt
    28321 gagacatact tatgctcatt gatgttgaat cggatttact ttcacttgat gatgttgagt
    28381 cggattcact ttcacttgta gaaccactta atgatgtgga tgtactaatg gaatcagatt
    28441 tacttgtact gattgaatca cttgtcgaca ttgatgtact taatgaatca gacttactat
    28501 cacttgtgga atcacttaat gatgttgaca aacttgtaga gtcagacaaa cttgtactcg
    28561 ttgacatact tagtgatgtt gaaacactct cgctcttaaa cgttgacgtt gattcactga
    28621 tacttgtcga tgttgaaatg gacgcactac cacttgttga attacttaat gatgttgatg
    28681 tgctaccaga ttctgatgta ctgtctgata atgacgtact ctcacttgtc gaactactca
    28741 ctgactctga tgttgattca gacgtacttt cacttaatga ttcacttaaa aaggcagatg
    28801 cactttgtga ttctgaatcg ctagtactat ttgattcact taatgatata aacgtgctct
    28861 ctgaagcaga tattgcttca cttatagagt cgctcgttga cgttgattca cttattgaat
    28921 cagactctga tgtacttaag cttgtggaat cactcataga tgttgatgta cgttctgaat
    28981 tacttgtact taacgatgtc gaagtactta ccgaaccaga tgtgctcgta gaagcacttt
    29041 gtgatattga ttcacttgat gcagttgatg ctgatttgct atcactcgtt gaatcactaa
    29101 acgacgttga tatgctcatt gaatcggatt gacttgcact cattgaacca gacgtacttt
    29161 gtgattccga agtacgtact gaagcacttg tcgacgttga tgtacttgtt gaacctgatt
    29221 gactagtact tagtgataat gacttactat cagaatcaga tgtactttgt gaatcactta
    29281 atgattctga tgtactacct gactgagacg tgctcattga actacttaca gacattgatt
    29341 tactgtctga tgcagataat gacccacttg tactgataga gtcacttact atctctgaag
    29401 tactcatcga gtctgatgta cttgttgaga cactttgtga tgctgctatg cttagtgatc
    29461 cagaaacaga accacttgtg ctcgtcgaat cgctcaatga ttctgatgta ctcatcgatt
    29521 ttgaatcact tgtacttaat gatattgatg tactttgtga atctgatttg cttgttgacg
    29581 cactttgaga gttggctatg ctatttgaaa tactgataga gtccgaggtg ctagctgact
    29641 cgctcaatga ttctgatgtg ctgattgcat tcgatgtgct gtcacttaat gatgctgatg
    29701 tactagacga ttctgatgta cttgttgata gactttgtga cttagacaag ctccccgatg
    29761 tactcatact taatgagtca ctgagtgacg ttgatgtacg caatgaatca gatgtacttg
    29821 ttgatagact ttcggatttt tcagtactgc tagagtttga aatagaatcg cttaatgatg
    29881 ttgatttact agctgaatcc gacatgcttg atgatacact ttgtgaattc actaaacttg
    29941 tgcttgttga gcttgataca ctattacttt cagatgtgct taatgactta gatgcactca
    30001 cagaatcaga taggcttaca cttgtcgatt tcgaggtact agctgatgta gataccacaa
    30061 tcgatcctga tgtactcgtt gatgcacttt gtgagtcagc tttacttgtt gagacacttt
    30121 gggattgttg tgtacttcct gatgttgata cggaatcact catgctattt cttgttactt
    30181 catatttaaa agttgttgtc gttttgttac cactcgcatc tgtagaaacg attgtaatag
    30241 tacttgtacc aatgtttgtt ggtgtaccac taatggtatt atttgtacta tcgaatgtta
    30301 gtccagatgg caatccagtc actgtattcg tcaccgcatt tccactgttg tcctgcgtag
    30361 caattttaat cggtgatatt ggcgaataca cttctgatga ttgatctcct attggtgtca
    30421 ctgttggtgc tgtcgtatcc acaacattta ttgtaaaagt tgtcgtcgat ttattgtttg
    30481 cttggtcagt agacacaact gtcactgttg attgaccaat tttcgttggc gtcccaatga
    30541 ttgaattcgt tgcactatca taacttaatc cgcttggtaa tcctgtaact gtatttgtca
    30601 cagtcccagt accattatcc gttgtagtca acacaacagg attcattgtt ttacccactt
    30661 caatggtttg attgcctaca gttacagttg gtgcttttac atcagtaaaa taatatgtca
    30721 ctgattgtcc agcattcgtc atttttacag ttttatttgt atcattataa gttgacgcat
    30781 atgaactatc gacggacgtg tagttatatc ctttagcagt caatgcagat tgctgattat
    30841 cgattgtcac gacttgatca acatttcctg aatatgtttt tggtggaata atatctttac
    30901 ctgttgttac atcaacgtat ctcacttgtg taacagcaga ctctgtatat tcgaatgttc
    30961 caaattgtac ttgttgtaaa tttgtcgcgc cacctgttga ggctgtcatt gataatgaaa
    31021 agttggtcgt accacttttt gcaatccaat ctgaaatatt acgtgtccat gtttgacctg
    31081 catacgttac agtcataact tttgtatcac cattataatt aatatcaaaa tcttggaacg
    31141 tgttgttcgt aggttgcact tttaacttcg cagcattatc agctgttgaa cttgatgtat
    31201 acgttgtcgc aacaccataa ctatctgttg ttacaaaggc accaaacgca cctccaccag
    31261 ctacattaga tgggtcagca ttcgcctttg cagctgaatt tggtttagat gtattgtgat
    31321 acgtatccaa tttgaagcca aatgcgttac ttaagccacc aatacctact gcggcaccgt
    31381 ttaaccctgt ttcacctaat acacctggcg aaaaggcaaa gccgatacca tctccaccat
    31441 ttccattacc ttcgtattta ttacctaaat ttacttttcc agaaaaatga aaactcttat
    31501 tagagtcaat acgtgttcct aatgtaatgg cacctttttg gctgtatgca tcctgtgtta
    31561 acgtcacaat accggtactt tgatcatagg tagcattacc tgacgttgtc atatattgtt
    31621 ttaagttatc tttattaact gtaattgtat tagcagttac tgcggttgtc gtcgctgctg
    31681 acgcaaatgt tgacatagct aagcgactga aagttcgaag ttttactggt gcggtgctag
    31741 ttgacgttgt gctagttttg tttaagttga ccgaagatgg cgttgtgctt tgtgaagtgt
    31801 tatttgatgc agtactttga tttgttgatg tattaattgg ttgttctgta cttgaagttg
    31861 aagctacaga tttagtatca gaacttgatg tagtattctt tgaggatgtt gattctgatg
    31921 tagatgtcaa tttttcttgt tgattgcttg tactattagt tgtcgaagtg accttttcag
    31981 acttttcact tgagactgtg tcactatttg atgtttgtac cgaactacta tttttcgtta
    32041 cacttgtgga atcggctgtt gatgttgatg cttcgattgt cgttgagttt tgattaccta
    32101 ctgtttcact ttgtgtgttt aattcagaag ttaatggtgc atcagaagcc gcaaaagctt
    32161 gctggtcatg caacatattt accgtgaatg caccaccaat aaccgccgta gttttcagtc
    32221 cgtatcccgt cattttttta ctaatgcttt gattatcttg actcactaaa ctatgactaa
    32281 taaatggtag ccccataatt ttgaacattt ctatttcttt aattccggat tttacccaat
    32341 tttttccaga tttataaagt cttactcttg ttttttcgtt tgctaagctg tcatgaaatt
    32401 ctttctgtct tttactcatg taataactcc ttgtatta
//
LOCUS       DAMZKC010000011        31585 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.011, whole genome shotgun sequence.
ACCESSION   DAMZKC010000011 DAMZKC010000000
VERSION     DAMZKC010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 31585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31585
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.011"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(213..908)
                     /locus_tag="POB55_000368"
     CDS             complement(213..908)
                     /locus_tag="POB55_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6499364.1"
                     /translation="MEGNLLQQLFNYYVTNFGYLWDLFFKHLLMSVYGVLFAALIGIP
                     LGILLARYTKLSGFVITIANIIQTVPVIAMLAILMLVMGLGSETVVLTVFLYALLPII
                     KNTYTGIASVDANIKDAGKGMGMTRNQVLRMIELPLSVSVIIGGIRIALVVAIGVVAV
                     GSFIGAPTLGDIVIRGTNATDGTTFILAGAIPIAIIAIVIDVLLRFLEKRLDPTTRHR
                     KNQSNHRPQSINM"
     gene            complement(908..1849)
                     /locus_tag="POB55_000369"
     CDS             complement(908..1849)
                     /locus_tag="POB55_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HDI6499365.1"
                     /translation="MKKIKYILVVFVLSLTVLSGCSLPGLGSKSTKNDVKITALSTSE
                     SQIISHMLRLLIEHDTHGKIKPTLVNNLGSSTIQHNALINGDANISGVRYNGTDLTGA
                     LKEAPIKDPKKAMIATQQGFKKKFDQTFFDSYGFANTYAFMVTKETAKKYHLETVSDL
                     AKHSKDLRLGMDSSWMNRKGDGYEGFKKEYGFDFGTVRPMQIGLVYDALNSEKLDVAL
                     GYSTDGRIAAYDLKVLKDDKQFFPPYAASAVATNELLRQHPELKTTINKLTGKISTSE
                     MQRLNYEADGKGKEPAVVAEEFLKKHHYFDKQKGGHK"
     gene            complement(1866..2501)
                     /locus_tag="POB55_000370"
     CDS             complement(1866..2501)
                     /locus_tag="POB55_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6499366.1"
                     /translation="MIEFLHEHGGQLMSKTLEHFYISIVALLLAIIVAVPIGILLSKT
                     KRTANIILTVAGVLQTIPTLAVLAIMIPIFGVGKTPAIVALFIYVLLPILNNTVLGVQ
                     NIDSNIKEAGKSMGMTQFQLMKDVELPLALPLIIGGIRLSSVYVISWATLASYVGAGG
                     LGDFIFNGLNLYDPLMIVTATVLVTALALGVDALLALVEKWVVPKGLKVSG"
     gene            complement(2498..3724)
                     /locus_tag="POB55_000371"
     CDS             complement(2498..3724)
                     /locus_tag="POB55_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6499367.1"
                     /translation="MLSIKHLTKIYSGNKKAVDDISLDIQSGEFIAFIGTSGSGKTTA
                     LRMINRMIEATEGQIEIDGKDVRSMNPVELRRSIGYVIQQIGLMPHMTIKENIVLVPK
                     LLKWTKEEKDKRAKELIKLVDLPESFLERYPAELSGGQQQRIGVVRALAAEQDIILMD
                     EPFGALDPITRDTLQDLVKTLQRKLGKTFIFVTHDMDEAIKLADKICIMSEGKVVQFD
                     TPDNILRHPANDFVRDFIGQNRLIQDRPNDKTVEGVMIKPITIQAEATLNDAVHIMRQ
                     KRVDTIFVVDSDNHLLGFLDIEDINQGIRGHKTLRDMMQQHIYTVQIDSKLQDSVRTI
                     LKRNIRNVPVVDDQQRLVGLITRANVVDIVYDTIWGDSEDTVQAEHVGEDTASSKVHE
                     QHTTNVKVRDIGDDKS"
     gene            complement(4051..4170)
                     /locus_tag="POB55_000372"
     CDS             complement(4051..4170)
                     /locus_tag="POB55_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499368.1"
                     /translation="MDVCPPVTMDACALLQKFEFCNNISHFRHFFAIKQPFER"
     gene            complement(4250..4390)
                     /locus_tag="POB55_000373"
     CDS             complement(4250..4390)
                     /locus_tag="POB55_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499369.1"
                     /translation="MSKAYRLYNNIQDDTKQPMAHAVGCFFRCACHGQHFDVGIPLQA
                     WE"
     gene            complement(4394..4993)
                     /locus_tag="POB55_000374"
     CDS             complement(4394..4993)
                     /locus_tag="POB55_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family protein"
                     /protein_id="HDI6499370.1"
                     /translation="MSEKQSNEQVEAFLNKESQWQEEYKYLRALIFNETELEEAYKWM
                     HPCYTLNNKNVVLIHGFKNYVALLFHKGAILEDKYHTLIQQTEKVQAARQLRFENLTE
                     IQARSEEIKYYLAEAIKAEKAGKKVEMKKTEEYVIPKELEAKFEEMPQLESSFYKLTP
                     GRQHQYIYHIGQAKRSETRQKRVEKYINQILEGKGMHDK"
     gene            5173..6810
                     /locus_tag="POB55_000375"
     CDS             5173..6810
                     /locus_tag="POB55_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HDI6499371.1"
                     /translation="MNKDKQLHNDKINLSQLVLLGLGSLIGSGWLFGAWEASSIAGPA
                     AIISWVLGFLVIGTIAYNYIEIGTMFPQSGGMSNYAQYTHGSLLGFIAAWANWVSLVT
                     IIPIEAVSAVQYMSSWPWHWAKPMRYLMENGSISTYGLLAVYLIIVIFSLLNYWSVKL
                     LTSFTSLISVFKLGVPMLTIIMLMLSGFDTSNYGHSASTFMPYGSAPIFAATTASGII
                     FSFNSFQTIINMGSEIKNPEKNIARGIAISLSISAVLYIILQSTFITSMPQSMLQHSG
                     WNGINFNSPFADLAILLGINWLAILLYIEAFVSPFGTGVSFVAVTGRVLRAMEKNGHI
                     PKFLGKMNEKYHIPRVAIIFNAIISMIMVTLFRDWGTLAAVISTATLVAYLTGPTTVI
                     ALRKMGPTMTRPFRAKILKVMAPLSFVLASLAIYWAMWPTTAEVILIIILGLPIYFFY
                     EYRMNWRNTKKQIGGSLWIIVYLIVLSILSFIGSKEFKGLNMIHYPFDFIVIIVVALI
                     FYYIGTTSSFESVYFRRATRINTKMRESLNNESKSSH"
     gene            7117..8469
                     /locus_tag="POB55_000376"
     CDS             7117..8469
                     /locus_tag="POB55_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase/lipase family protein"
                     /protein_id="HDI6499372.1"
                     /translation="MKINTTGGQIHGITQDGLDIFLGIPYAEPPVHDNRFKHSTLKTQ
                     WSEPIDATEIQPIPPQPDNKLEDFFSSQSTTFTEHEDCLYLNIWKQHNDQTKKPVIIY
                     FYGGSFENGHGTAELYQPAHLVQNNDIIVITCNYRLGALGYLDWSYFNKGFHSNNGLS
                     DQINVIKWVHQFIESFGGDANNITLMGQSAGSMSILTLLKIPDIEPYFHKVVLLSGAL
                     RLDTLKSARNKAQYFQKLMLDYLDTDDVTSLSTDDILMLMAKLKQSRGPSKGLDLIYA
                     PIKTDYIQNNYPTTKPIFACYTKDEGDIYITSEQKKLSPQRFIDIMELNDIPLKYEDV
                     QTAKQQSLAITHCYFKQPMKQFLQQLNIQDSNAQLWLAEFAWHDTSSAHYRSAYHILD
                     MVFWFGNLQILAAHQYPTTAHLKFLSRQMQNDLANFAKSGKMPWPMYHNERRYYRTYQ
                     "
     gene            complement(8531..9718)
                     /locus_tag="POB55_000377"
     CDS             complement(8531..9718)
                     /locus_tag="POB55_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6499373.1"
                     /translation="MSIMRLFTFILSIFIVGMVEMMVAGIMNLMSQDLHVSEAVVGQL
                     VTMYALTFAICGPILVKLTNRFSSRPVLLWTLLIFIIGNGIIAVAPNFSILVVGRIIS
                     SAAAALIIVKVLAITAMLSAPKNRGKMIGLVYTGFSGANVFGVPIGTVIGDLVGWRYT
                     FLFLIIVSIIVGFLMMIYLPKDQEIQRGPVNHETPSHENHVTSKILRPAEVAKYLIIT
                     FLVLIANSVTFVFINPLILSNGHDMSFVSLALLVNGIAGVIGTSLGGIFSDKITSKRW
                     LMISVSIFIVMMLLMNLILPGSGLLLAGLFIWNIMQWSTNPAVQSGVIQHVEGDTSQV
                     MSWNMSSLNAGIGVGGIIGGLVMTHVSVQAITYTSAIIGALGLIVVFTLKNNHYAKTF
                     KSS"
     gene            complement(10114..10890)
                     /gene="fetB"
                     /locus_tag="POB55_000378"
     CDS             complement(10114..10890)
                     /gene="fetB"
                     /locus_tag="POB55_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HDI6499374.1"
                     /translation="MSNTALGLTALLLVIPIIISYKEGLHIIKDLIVATLRAIVQLII
                     LGFLLHYIFKINDKWLLILCVLVIIINASWNTISRASPVMHHVFWISFLAIFIGTALP
                     LAGTIATGAIQFTANEVIPIGGMLANNGLIAINLAYQNLDRAFVQDGTNIESKLSLAA
                     TPKLASKGAIRESIRLAIVPTIDSVKTYGLVSIPGMMTGLIIGGVPPLQAIKFQLLVV
                     FIHTTATIMSALIATYLSYGQFFNARHQLVARNTDVKSES"
     gene            complement(10883..11545)
                     /locus_tag="POB55_000379"
     CDS             complement(10883..11545)
                     /locus_tag="POB55_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDI6499375.1"
                     /translation="MLLEIKDLVYKASDRIILDHISLKVDKGESIAIIGPSGSGKSTF
                     QKQICNLISPTSGELYFKGKPYNDYDPEELRQRISYLMQQSDLFGETIEDNMIFPSLA
                     RNDKFDRKRAKQLIKDVGLGHYQLSSEVENMSGGERQRIAIARQLMYTPDILLLDEST
                     SALDVHNKEKIENIIFKLVEQDVAIMWITHSDDQSMRHFQKRITIVDGKISKVEELNQ
                     HE"
     gene            complement(11793..12869)
                     /locus_tag="POB55_000380"
     CDS             complement(11793..12869)
                     /locus_tag="POB55_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HDI6499376.1"
                     /translation="MNQSVKLLKHLTDVNGIAGYEMQVKEAMRNYIEPVSDQIIEDNL
                     GGIFGKKNAENGQYSIMISGHMDEVGFMVTKIDKHGFISFTPVGGWWNQVMLSQKVTI
                     TTDSGKEIRGIIGSKPPHVLTPEERKKPMEIKNMFIDIGVSSKEEAEEAGVEVGNMVT
                     PYSEFEVLANDKYLTAKAFDNRYGCALAIEVLKRLKDENIGINLYSGATVQEEVGLRG
                     AKVAANTIKPDLAIAVDVGIAYDTPGMSGQTSDSKLGGGPVVIMMDATSIAHQGLRKH
                     IKDVAKEHNIEVQWDTTPGGGTDAGSIHVANEGIPTMTIGVTLRYMHSNVSVLNVDDY
                     ENSIRLVTEIVRSLNDESYKNIMW"
     gene            complement(13225..13491)
                     /locus_tag="POB55_000381"
     CDS             complement(13225..13491)
                     /locus_tag="POB55_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HDI6499377.1"
                     /translation="MSNYTVKIKNSAKSDLKKIKHSYLKKSFLEIVETLKNDPYKITQ
                     SFEKSEPKYLERYSRRINHQHRVVYTVDDRNKEVLILSAWSHYD"
     gene            complement(13491..13748)
                     /locus_tag="POB55_000382"
     CDS             complement(13491..13748)
                     /locus_tag="POB55_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDI6499378.1"
                     /translation="MIITSPTEARKDFYQLLKNVNNNHEPIYISGNNAENNAVIIGLE
                     DWKSIQETIYLESTGTMDKVREREKDNSGTTNIDDIDWDNL"
     gene            14107..14562
                     /locus_tag="POB55_000383"
     CDS             14107..14562
                     /locus_tag="POB55_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1307 domain-containing protein"
                     /protein_id="HDI6499379.1"
                     /translation="MKKLCSLIVVALVCIIALSACGKEQTKTYEGDVSGKHVLTSITY
                     KDDKVLKQSTINTIKYDDLGMDKDEAKKLFAKSESIFKDLKGVKYKVDYKDKKAIEHL
                     DIDYTEVDMKKLNKRLGVSTKENKDISFEKLEKQLKHRGLKEKDKMDDK"
     gene            complement(14730..16307)
                     /locus_tag="POB55_000384"
     CDS             complement(14730..16307)
                     /locus_tag="POB55_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HDI6499380.1"
                     /translation="MTFLTVMQFIVNIIVVGFLLTVIVIGLIWLIKDKRQSQHSVLRN
                     YPLLARIRYISEKMGPELRQYLFSGDNEGKPFSRNDYKNIVLAGKYNSRMTSFGTTKD
                     YQDGFYIQNTMFPMQRNEISVDNTTLLSTFIYKIANERLFSREEYRVPTKIDPYYLSD
                     DHAIKLGERLKHPFILKRIVGQSGMSYGALGKNAITALSKGLAKAGTWMNTGEGGLSE
                     YHLKGNGDIIFQIGPGLFGVRDKEGNFSEGLFKEVAQLSNVRAFELKLAQGAKTRGGH
                     MEAEKVNEEIAKIRNVEPYKTINSPNRYEFIHNAEDLIRFVDQLQQLGQKPVGFKIVV
                     SKVSEIETLVRTMVELDKYPSFITIDGGEGGTGATFQELQDGVGLPLFTALPIVSGML
                     EKYGIRDKVKLAASGKLVTPDKIAIALGLGADFVNIARGMMISVGCIMSQQCHMNTCP
                     VGVATTDAKKEKALIVGEKQYRVTNYVTSLHEGLFNIAAAVGVSSPTEITADHIVYRK
                     VDGELQTIHDYKLKLIS"
     gene            16503..17252
                     /locus_tag="POB55_000385"
     CDS             16503..17252
                     /locus_tag="POB55_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499381.1"
                     /translation="MSNTNKHYIEEEYATEQSRFFKRDIGFIFLYIFLVNILPIIIVF
                     LTFGLVAVFTQQAPWGFSFPISMNISYLLAVIISLAIFRRMHEDAFIPMIRSQIVASA
                     KYKWHILISFTLSVVLFLLLNHFNIFALNVDAFTVFDIYPLIKGYGSISLIAIFSLTI
                     ILNTLICQLIFRHILIQELSRLIPLKITIVLSVILETLYYYPYINTWWEFIPALILAS
                     SATYLYLKSYRNFMVSYFYQLAVILVLHIFM"
     gene            complement(17614..18807)
                     /locus_tag="POB55_000386"
     CDS             complement(17614..18807)
                     /locus_tag="POB55_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6499382.1"
                     /translation="MKGAMAWPFLRLYILTLMFFSANAILNVFIPLRGHDLGATNTVI
                     GIVMGAYMLTAMVFRPWAGQIIARVGPIKVLRIILIINAIALIIYGFTGLEGYFVARV
                     MQGVCTAFFSMSLQLGIIDALPEEHRSEGVSLYSLFSTIPNLIGPLVAVGIWNANNIS
                     LFAIVIIFIALTTTFFGYRVTFAEQEPDTSDKIEKMPFNAVTVFAQFFKNKELLNSGI
                     IMIVASIVFGAVSTFVPLYTVSLGFANAGIFLTIQAIAVVVARFYLRKYIPSDGMWHP
                     KYMVSVLSLLVIASFVVAFGPQVGAIIFYGSAILIGMTQAMVYPTLTSYLSFVLPKVG
                     RNMLLGLFIACADLGISLGGALMGPISDLVGFKWMYLICGMLVIVIMIMSLLKKPTPR
                     PASSL"
     gene            complement(18819..19568)
                     /locus_tag="POB55_000387"
     CDS             complement(18819..19568)
                     /locus_tag="POB55_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6499383.1"
                     /translation="MIKIKDVEKSYQSAHVFKRRRTPIVKGVSFECPIGATIAIIGES
                     GSGKSTLSRMILGIEKPDKGCVTLNDQPMHKKKVRRHQIGAVFQDYTSSLHPFQTVRE
                     ILFEVMCQCDGQPKDVMEVQAITLLEEVGLSKAYMDKYPNMLSGGEAQRVAIARAICI
                     NPKYILFDEAISSLDMSIQTQILDLLIHLRETRQLSYIFITHDIQAATYLCDQLIIFK
                     NGKIEEQIPTSALHKSDNAYTRELIEKQLSF"
     gene            complement(19561..20376)
                     /locus_tag="POB55_000388"
     CDS             complement(19561..20376)
                     /locus_tag="POB55_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6499384.1"
                     /translation="MTLLTVKHLTITDTWTDQPLVSDVNFTLTKGETLGVIGESGSGK
                     SITCKSIIGLNLERLGVTGEIIFDGTSMLSLSESQLKKYRGKDIAMVMQQGSRAFDPS
                     TTVGKQMFETMKVHTSMSTQEIEKTLIEYMDYLSLKDSKRILKSYPYMLSGGMLQRLM
                     IALALALKPKLIIADEPTTALDTITQYDVLEAFIDIKKHFDCAMIFISHDLTVINKIA
                     DRVVVMKNGQLIEQGTRESVLHHPEHVYTKYLLSTKKKINDHFKHVMRGDVHD"
     gene            complement(20373..21242)
                     /locus_tag="POB55_000389"
     CDS             complement(20373..21242)
                     /locus_tag="POB55_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6499385.1"
                     /translation="MIILKRLLQDKGAVIALGIIVLYVFLGLAAPLVTFYDPNHIDTA
                     NKFAGMSFQHLLGTDHLGRDILTRLIYAIRPSLLYVFVALFVSVLIGSILGFLSGYFQ
                     GFVDALIMRACDVMLAFPSYVVTLALIALFGMGAENIIMAFILTRWAWFCRVIRTSVM
                     QYTASDHVRFAKTIGMNDMKIIHKHIMPLTLADIAIISSSSMCSMILQISGFSFLGLG
                     VKAPTAEWGMMLNEARKVMFTHPEMMFAPGIAIVIIVMAFNFLSDALQIAIDPRISSK
                     DKLRSVKKGVVQS"
     gene            complement(21239..22174)
                     /locus_tag="POB55_000390"
     CDS             complement(21239..22174)
                     /locus_tag="POB55_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6499386.1"
                     /translation="MFKFILKRIALMFPLVIVVSFMTFLLTYITNENPAVTILHAQGT
                     PNVTPELIAETNEKYGFNDPLLIQYKNWLLEAMQFNFGTSYITGDPVAERIGPAFMNT
                     LKLTIISSVMVMITSIILGVVSALKRGKFTDRAIRSVAFFLTALPSYWIASILIIYVS
                     VKLNILPTSGLTGPESYILPVIVITIAYAAIYFRNVRRSMVEQLNEDYVLYLRASGVK
                     SITLMLHVLRNALQVAVSIFCMSIPMIMGGLVVIEYIFAWPGLGQLSLKAILEHDFPV
                     IQAYVLIVAVLFIVFNTLADIINALLNPRLREGAR"
     gene            complement(22187..23785)
                     /gene="cntA"
                     /locus_tag="POB55_000391"
     CDS             complement(22187..23785)
                     /gene="cntA"
                     /locus_tag="POB55_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylopine-dependent metal ABC transporter
                     substrate-binding protein CntA"
                     /protein_id="HDI6499387.1"
                     /translation="MRKLTKMSAMLLASGLILTGCGGNKGLEEKKENKQLTYTTVKDI
                     GDMNPHVYGGSMSAESMIYEPLVRNTKDGIKPLLAKKWDVSEDGKTYTFHLRDDVKFH
                     DGTPFDADAVKKNIDAVQQNKKLHSWLKISTLIDNVKVKDKYTVELNLKEAYQPALAE
                     LAMPRPYVFVSPKDFKNGTTKDGVKKFDGTGPFKLGEHKKDESADFNKNDQYWGEKSK
                     LNKVQAKVMPAGETAFLSMKKGETNFAFTDDRGTDSLDKDSLKQLKDTGDYQVKRSQP
                     MNTKMLVVNSGKKDNAVSDKTVRQAIGHMVNRDKIAKEILDGQEKPATQLFAKNVTDI
                     NFDMPTRKYDLKKAESLLDEAGWKKVKDSDVRQKDGKNLEMAMYYDKGSSSQKEQAEY
                     LQAEFKKMGIKLNINGETSDKIAERRTSGDYDLMFNQTWGLLYDPQSTIAAFKAKNGY
                     ESATSGIENKDKIYNSIDDAFKIQNGKERSGAYKNILKQIDDEGIFIPISHGSMTVVA
                     PKDLEKVSFTQSQYELPFNEMQYK"
     gene            complement(23925..25229)
                     /gene="cntM"
                     /locus_tag="POB55_000392"
     CDS             complement(23925..25229)
                     /gene="cntM"
                     /locus_tag="POB55_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_096659686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylopine dehydrogenase CntM"
                     /protein_id="HDI6499388.1"
                     /translation="MSKLLMIGTGPVAIQLANICYLKSDYEIDMVGRASTSEKSKRLY
                     QAYKKEKQFEVKIQNEAHQHLEGKFEINRLYKDVKNVNGEYETVVMACTADAYYDTLQ
                     QLSLETLQSVKHVILISPTFGSQMIVEQFMSKFNKDIEVISFSTYLGDTRIVDKEAPN
                     HVLTTGVKKKLYMGSTHSNSTMCQRISALAEQLKIQLEVVESPLHAETRNSSLYVHPP
                     LFMNDFSLKAIFEGTDVPVYVYKLFPEGPITMTLIREMRLMWKEMMAILQAFRVPSVN
                     LLQFMVKENYPVRPETLDEGDIEHFEILPDILQEYLLYVRYTAILIDPFSQPDENGHY
                     FDFSAVPFKQVYKNEQDVVQIPRMPSEDYYRTAMIQHIGKMLGIKTPMIDQFLTRYEA
                     SCQAYKDMHQDQHLSSQFNTNLFEGDKALVTKFLEINRTLSQ"
     gene            complement(25222..26040)
                     /gene="cntL"
                     /locus_tag="POB55_000393"
     CDS             complement(25222..26040)
                     /gene="cntL"
                     /locus_tag="POB55_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-histidine (S)-2-aminobutanoyltransferase CntL"
                     /protein_id="HDI6499389.1"
                     /translation="MNNFNNEIKLILQQYLEKFEAHYERVLQDDQYIEALETLMDDYS
                     EFILNPIYEQQFNAWRDVEEKAQLIKSLQYITAQCVKQVEVIRARRLLDGQASTTGYF
                     DNIEHCIDEEFGQCSIASNDKLLLVGSGAYPMTLIQVAKETGASVIGIDIDPQAVDLG
                     RRIVNVLAPNEDITITDQKVSELKDIKDVTHIIFSSTIPLKYSILEELYDLTNENVVV
                     AMRFGDGIKAIFNYPSQETAEDKWQCVNKHMRPQQIFDIALYKKAAIKVGITDV"
     gene            complement(26051..26872)
                     /gene="cntK"
                     /locus_tag="POB55_000394"
     CDS             complement(26051..26872)
                     /gene="cntK"
                     /locus_tag="POB55_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine racemase CntK"
                     /protein_id="HDI6499390.1"
                     /translation="MNRQVIEFSKYNPSGNMTILVHSKHDASEYASIANQLMAATHVC
                     CEQVGFIESTQNDDGNDFHLVMSGNEFCGNATMSYIHHLQESHLLKDQQFKVKVSGCS
                     DLVQCAIHDCQYYEVQMPQAHRVVPTTINMGNHSWKAIEIIYETYVHYVIPVKQVTTE
                     IQHLVEAFVREQQWSHKYKTVGMMLFDEQRQFLQPLIYIPEIQSLIWENSCGSGTASI
                     GVFNNYQRNDACKDFTVHQPGGSILVTSKRCHQLGYQTSIKGQVTTVATGKAYIE"
     gene            complement(27747..28109)
                     /locus_tag="POB55_000395"
     CDS             complement(27747..28109)
                     /locus_tag="POB55_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499391.1"
                     /translation="MLKEKESFRLLYQAIREIADKIGDNQLETNSVSLLLLDFDFEHE
                     VFDELYLAILKYLNTVSIENISHSELLNLIENTIPEDRDINTFVKNKIIIGFANNYFP
                     ELQVLANEIKSDMASSLK"
     gene            complement(28372..29190)
                     /locus_tag="POB55_000396"
     CDS             complement(28372..29190)
                     /locus_tag="POB55_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001620216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDI6499392.1"
                     /translation="MKRLENKVAVVTGASTGIGQASAIALAQEGAYVLAVDIAEAVSE
                     TVDKIKSNGDKAKAYIVDIASEQQIDNFASEIREQIGHVDVLFNNAGVDNAAGRIHEY
                     PTDVYDKIMNVDMRGTFLMTKMMLPLMMTKGGSIVNTSSFSGQAADLYRSGYNAAKGA
                     VINFTKSIAIEYGRDGIRANAIAPGTIETPLVDKLTGTSEDKEGKAFRENQKWMTPLG
                     RLGKPEEVGKLVVFLASDESSFITGETIRIDGGVMAYTWPGEMLSDDSWKRTLE"
     gene            29425..30963
                     /locus_tag="POB55_000397"
     CDS             29425..30963
                     /locus_tag="POB55_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HDI6499393.1"
                     /translation="MTSKHQQKGSIVNRFLNSVEKIGNKLPDPSVLFFLMCVGLAIMT
                     WFISLFNVSVKHPGTHQTIYIKNIISHDGFTMIMNDTIKNFSEFPALGLVLAVMIGIG
                     VAEKTGYFDKLMISVVNRAPRFLILPTIILIGILGSTAGDAATIILPPLAAMLFIKIG
                     YHPIAGLTMAYASAVGGFAANIVVGMQDALVYSFTEPATRIVSDSIKTNVAMNWYFIA
                     ASVVVLLPTILLVTTKLIIPRLGQYDDRLMHDDHEETSSHITDKEAHALKWANISFIV
                     TIILLIITAIPEHSFLRNAKTGSLLDDAPLINGVGLIILVVFLVPGLVYGILSKEIKN
                     TKDLGKMFGDAVGSMGTFIVIVFFAAQLLAYLKWSNLGIIAAVKGAKLLEHQNGIVLI
                     LGIIVLSAMVNMLIGSASAKWGILGPIFVPMLILIGFHPAFTQVIYRVGDSITNPITP
                     MMPYLPLLLTYAQKYDKRMKLGALLSSLMPYSIALSIVWTVFVIIWFLLGIPVGPGGP
                     IFVK"
     gene            complement(31075..31497)
                     /locus_tag="POB55_000398"
     CDS             complement(31075..31497)
                     /locus_tag="POB55_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDI6499394.1"
                     /translation="MPYNYKKQNGELMSVMSQGEKFIHQSPVNDELSALIKLLISKIN
                     GCHYCVDIHKKELKELGVTQMKIDEVLSFRHLDLFTDQEKVTLEFAEMLNSIKDFKKF
                     EIIDRLKSFYDEEQIIDLVFVVNQINGWNRLNIISDKL"
BASE COUNT        10042 a         6125 c         4662 g        10756 t
ORIGIN      
        1 aattataaat aaaacatctc attttttagc atttttacaa taattaacgc tttttatcca
       61 aaaactttca tgataataat tgccatttta gtgtaaacgc atacaaacac aacctataaa
      121 aaggctactc aaagattatt tgagctcctt gagtagcctc attttttata aatcatgaaa
      181 cgaaatcgct aaattataaa catcttctac tattacatat taatactttg cggccgatga
      241 ttagattgat ttttacgatg tcgtgttgtt gggtctaatc gtttttctaa aaatcttaat
      301 agtacatcaa tgacgattgc aatgatagca atcggaatcg cacctgctaa aataaacgtt
      361 gtgccatccg tcgcatttgt accacgaatc acaatgtcac caagcgtagg tgctcctata
      421 aatgatccaa cggcaacaac acctatcgca acaaccaagg caatacgaat gccaccgata
      481 ataaccgaaa cagataacgg taattcaatc attcgtagca cttgattgcg tgtcattccc
      541 atacctttgc cagcatcctt aatattcgca tcaacactag ctataccagt ataagtgttt
      601 tttataattg gaagtaacgc atataaaaac actgttaaaa ctactgtttc tgaacctaag
      661 cccatgacta acattaaaat agctaacatt gcaatgactg gaactgtttg aattatattt
      721 gcaattgtaa ttacaaatcc agaaagtttt gtgtatcttg caagcaagat tcccaatgga
      781 ataccaatta aagctgcaaa cagcacacca tagacagaca ttaataagtg tttgaaaaat
      841 aaatcccata gatagccaaa gttcgtaaca taataattga ataattgctg taataaatta
      901 ccttccatta cttatgacca cctttctgtt tatcaaaata gtggtgtttt ttcaaaaatt
      961 cttctgcgac gacagcaggt tctttacctt taccatccgc ttcataattc aagcgctgca
     1021 tctctgaagt tgaaatcttt cctgtcaact tattaatcgt cgttttaagt tctgggtgtt
     1081 ggcgtaataa ttcatttgtt gcaacagcac tcgcagcata aggtgggaaa aattgtttat
     1141 catctttaag tactttcaaa tcatacgcgg caattcgacc atctgtagaa taacctaatg
     1201 caacgtctaa cttctctgag tttaatgcgt cgtagactag acctatttgc attggtctca
     1261 ctgtaccaaa gtcgaaacca tactcttttt taaatccttc atagccatcg cctttacgat
     1321 tcatccatga actatccata cctaaacgta aatctttact atgctttgct aaatctgaaa
     1381 ctgtctctaa atgatatttt ttagcagttt ccttcgttac catgaatgca tacgtattcg
     1441 caaaaccata cgaatcaaaa aacgtttgat caaatttctt tttaaatcct tgttgtgtgg
     1501 ctatcattgc tttcttagga tctttaattg gtgcttcctt caaagctccc gttaaatctg
     1561 tgccattata tctaacacct gatatattag catccccatt aattaaggca ttatgttgaa
     1621 tcgtacttga ccctaaatta tttactaatg ttggctttat cttaccatgt gtatcatgct
     1681 ctattaacaa ccgtaacata tgtgaaataa tttgcgattc gcttgttgat aatgctgtaa
     1741 ttttgacatc atttttcgtg ctcttactac ctagtccggg caaactacat ccagataata
     1801 cggtaagcga taagacaaac acgacaagta tatatttaat tttcttcatt atcttagcct
     1861 cctaattatc cagatacttt taagcctttg ggaactaccc atttttcaac taaagctaat
     1921 aaggcatcaa cacctaatgc tagtgcagta acgagtaccg ttgcagttac aatcatcagt
     1981 ggatcatata aatttaaacc attgaaaatg aaatcaccta atccacccgc acctacataa
     2041 cttgcaagtg tagcccaact aattacatac acagacgaca aacgaatgcc accaatgata
     2101 agcggtaatg ctaacggcaa ttcaacatcc ttcatcaatt gaaattgtgt cattcccata
     2161 ctttttccag cttctttaat gttgctatca atattttgaa caccgagtac cgtgttattt
     2221 aaaataggta ataatacata aataaatagc gctacaattg caggcgtttt accaacacca
     2281 aaaatcggta tcataatagc aagtacagct agtgttggaa tagtttgtaa gacacctgcc
     2341 acagttaata taatattggc agttcgcttt gtttttgata ataaaatgcc tataggtact
     2401 gcaacaatga tggcaagtaa taatgccact atagaaatat aaaaatgttc cagtgttttc
     2461 gacatcaact gtccaccatg ttcatgtagg aactcaatca tgatttatca tctcctatgt
     2521 cacgtacttt gacattagta gtgtgttgct catgcacttt tgaggacgca gtgtcttccc
     2581 ccacatgttc tgcttgcact gtatcctcac tatcgcccca aatcgtgtca tatacaatat
     2641 caacaacatt ggcacgcgta atcagtccta ctaaacgctg ttgatcatct acaacaggaa
     2701 catttctgat atttcttttt aaaatcgtgc gtacagaatc ttgcaatttc gaatcaattt
     2761 gaacggtata aatatgttgt tgcatcatat cacgcaaagt tttgtgtcct cgtatacctt
     2821 gattaatatc ctcaatgtct aagaatccaa gtaaatggtt gtcactatca acgacaaaaa
     2881 tagtatcaac acgtttttgt ctcataatat gaacggcgtc attcagtgtt gcttctgcct
     2941 gtatcgtgat tggtttaatc attacacctt ctacagtctt gtcattggga cggtcttgaa
     3001 tcagtctatt ttgtcctata aaatcacgta caaaatcatt tgcgggatgt cttaaaatat
     3061 tgtctggtgt atcaaattgc accaccttac cttctgacat aatacaaatt ttgtctgcta
     3121 atttaatcgc ttcatccata tcatgtgtta caaagataaa cgtcttgcct aattttcgtt
     3181 gtaacgtttt aactaaatct tgtaacgtat ctctcgtaat aggatccaat gcaccaaaag
     3241 gttcatccat taaaataata tcttgttcgg ccgcaagtgc tcttacgaca ccgatacgtt
     3301 gttgttgccc acctgatagt tctgctggat aacgctctaa aaatgactcc ggtaaatcca
     3361 caagtttaat taattccttt gcacgtttat ccttttcttc tttagtccat ttcaacaatt
     3421 tgggtaccaa tacaatattc tctttaatcg tcatatgagg cattaaccca atttgttgaa
     3481 taacataacc aatgcttcga cgcaattcga caggattcat actccgaaca tctttaccat
     3541 caatttcaat ttgtccttct gtcgcttcaa tcatacggtt tatcattctt aaagcagtcg
     3601 ttttaccact tccactggtt ccaataaatg cgataaattc cccagattga atatctaaag
     3661 agatgtcatc tactgccttt ttattaccag aataaatttt cgttaaatgc ttaatactta
     3721 acataatcat ttctccttcc tcataaaaaa tatgacgaat gatacgcata aaataaaaag
     3781 accatcatcg caattgttaa acaaatagag tcataagtta aacaagtaca catcatggtc
     3841 ttgattatgt agaattttat atttttcatc tgtaatgtga atacttaaca gtaaacggcg
     3901 ctatgtatgt tgaatttcta ccgattactt tgtttgcaca ttcgtatatt cgacatttaa
     3961 aactaacata ctctatatga tgttattaaa cattattatt ttatctttgg caactaaata
     4021 gataactcat attttttaaa agcgaattta ttatcgctca aatggttgtt ttatagcaaa
     4081 aaagtgtcgg aaatgtgaaa tattattgca aaactcgaat ttttgtaata atgcacacgc
     4141 atccattgtt acaggcggac aaacgtccat cagccactca caaagtcatc cttatatttt
     4201 aatattttca tcattcattg tagacaccct tgccttttgc taacatttcc tactcccaag
     4261 cttgtaacgg aattccaacg tcaaagtgtt gcccatgaca ggcacaccta aaaaaacaac
     4321 caacagcatg agccattggt tgtttcgtgt catcttgtat gttgttgtat aaccggtatg
     4381 ctttactcat taattactta tcatgcatcc ctttaccttc tagtatttgg ttaatatact
     4441 tttcaacacg cttttgtctt gtttcactgc gtttagcttg tccaatatga tatatatatt
     4501 gatgttgtct gcctggcgtt aatttataaa atgaagactc taactgtggc atttcttcga
     4561 acttagcttc taactcttta ggaataacat attcctctgt tttcttcatt tcaacttttt
     4621 taccagcttt ttcagcttta attgcttcag ctagataata tttaatttct tcgctacgtg
     4681 cttgaatctc tgttaaattt tcaaatcgta actgacgagc tgcttgcacc ttttcagtct
     4741 gttgaatgag tgtatgatat ttatcctcca aaatggcacc tttatgaaat aatagtgcaa
     4801 cataattttt gaagccatgg ataagtacta catttttatt attcaacgtg taacaaggat
     4861 gcatccattt atacgcttcc tctaattctg tttcattaaa gattaacgct ctcaaatatt
     4921 tatattcttc ttgccattga ctctctttat ttaaaaatgc ttcaacttgt tcatttgatt
     4981 gtttctcact catagtcatc acttccattt tgtttttcaa tacacattgt attatatatc
     5041 ctaattttat aaaaaactaa ttttacaaat gcttttgcgt tcttacaaaa aatgcatttg
     5101 actattatta taataagcgt ataattgtcg catattattt tttgtatttt tggcaataac
     5161 gaaggagtat ttatgaataa agacaagcaa ttgcacaacg acaaaatcaa tctatcccaa
     5221 ttagtcttat tagggttagg ctctttaata ggatctggtt ggctatttgg tgcgtgggaa
     5281 gcatcatcaa tagctggacc agcagcaatc atatcatggg ttcttggatt cctagtcatt
     5341 ggaaccattg cctataacta cattgaaatc ggcacaatgt ttcctcaatc aggtggcatg
     5401 agtaactatg cccagtatac acatggctca ttattaggct ttattgctgc ttgggcgaat
     5461 tgggtgtctt tggtgacaat aatacctatc gaagctgtgt cagctgttca atatatgagt
     5521 tcttggccgt ggcattgggc gaaaccaatg agatatttaa tggaaaatgg ctctattagc
     5581 acatacggat tgctagctgt atatctcatc attgttattt tttcattatt aaactattgg
     5641 tccgtaaaac ttttaacatc atttacgagt ttaatttctg tatttaaatt aggcgtaccc
     5701 atgttaacca tcatcatgtt gatgctatca ggattcgaca cttcaaatta cggccattcg
     5761 gcaagcacat ttatgcctta cggaagtgca ccgatttttg ctgcaacaac agcatcaggg
     5821 attatttttt cattcaattc attccagaca attattaata tgggttcaga aattaaaaat
     5881 cctgaaaaaa atatcgcaag aggcatcgct atctcactgt caatcagtgc agtgttgtac
     5941 atcattttac aaagtacgtt tatcacttct atgcctcaat caatgttaca acatagtgga
     6001 tggaatggca tcaacttcaa ttcaccattt gctgatttag ctatcttatt aggaattaat
     6061 tggctcgcaa ttttactata tattgaagct tttgtatcac cattcggtac tggcgtgtca
     6121 tttgtcgccg ttacaggtcg agttttacga gcaatggaga aaaatggaca tatccctaaa
     6181 tttcttggga agatgaatga aaaatatcat atcccacgtg tagcaatcat ctttaatgcc
     6241 atcattagta tgattatggt tacattattt agagattggg gtacgctagc agcagttatt
     6301 tctactgcaa ctttagtagc ctatttaact ggcccaacga cagtgattgc attaagaaaa
     6361 atgggaccaa caatgactcg tccatttaga gcaaaaattt taaaagtaat ggcaccatta
     6421 tcatttgtat tagcttcatt agctatatat tgggcaatgt ggccaacaac ggctgaagtt
     6481 attttaatca ttatacttgg attaccaatc tacttcttct atgaataccg tatgaattgg
     6541 cgtaatacaa agaaacaaat tggtggtagc ttatggatta ttgtatattt aatcgtgcta
     6601 tcaatactgt catttatagg aagcaaagaa tttaaaggct taaatatgat tcactatcca
     6661 tttgacttta tcgttattat tgttgtggcg cttatattct attacatcgg tacaacgagt
     6721 tcatttgaaa gcgtctattt ccgtcgcgca acacgaatca atacgaagat gcgtgagtca
     6781 ctaaataatg aatcaaagtc atctcattaa atattttaaa atacagtgcc atatatgatt
     6841 tcaacacaca cattaaccaa ccattgattt caacatcttg gttggttttt tattttgaaa
     6901 atcggttata aataactaac ataacaagat ggcgagagtg atattgtaat gaaatgtcac
     6961 tgtagaagaa catcatataa tgctgaaaat ggtttgagtt aaaacagata tcaaactcgt
     7021 ctgattcagt cacaaaattg tcttgttata cttgtcacct atcatctata gaccgtggta
     7081 tgattaaatt ggggatgata aaggaggtta ataaatatga agattaatac tacaggtggt
     7141 caaattcatg gtattacaca agatggttta gatatcttct taggcattcc ttatgcagaa
     7201 ccaccagttc atgacaatcg ctttaaacat tctacgttaa aaacacaatg gtcagagcca
     7261 attgatgcaa ctgaaataca acccatccca ccgcaaccag acaacaaatt agaagatttt
     7321 ttctcctcac aatctacaac ttttactgaa catgaagact gtttatatct aaatatttgg
     7381 aaacaacata atgatcagac gaagaaacct gtcatcattt atttttatgg tggtagtttt
     7441 gaaaatggtc atggtacagc cgaactctat caaccggcac atttagtaca aaataacgac
     7501 attatcgtta ttacatgcaa ttatcgttta ggcgcattag gatatttaga ctggtcatat
     7561 tttaataaag gttttcattc caataatggc ctttcagatc aaatcaatgt cataaaatgg
     7621 gtgcatcaat ttattgaatc cttcggtggc gacgctaata acattacttt aatgggtcag
     7681 tctgcaggca gtatgagcat tttgacttta cttaaaatac ctgacattga gccatacttc
     7741 cataaagtcg ttctactaag tggcgcacta cgattagaca cccttaagag tgcacgcaat
     7801 aaagcacaat atttccaaaa attgatgctc gattatttag atacagatga tgttacatca
     7861 ttatcgacag atgatattct tatgctgatg gcgaagctaa aacaatctcg aggaccttct
     7921 aaagggcttg atttaatata tgcgcctatt aaaacagatt atatacaaaa taattatcca
     7981 acaacgaaac caatttttgc atgttataca aaagatgaag gcgatattta tattactagt
     8041 gaacagaaaa aattatcgcc gcaacgcttt atcgacatta tggaattaaa tgatattcct
     8101 ttaaaatacg aagatgttca gacggcgaag caacaatctt tagcgattac acattgttat
     8161 ttcaaacagc cgatgaagca atttttacaa caactcaata tacaagattc aaacgcacaa
     8221 ctatggcttg ctgaatttgc atggcatgat acttctagcg cacactatcg cagtgcttac
     8281 catattctag atatggtgtt ttggttcgga aacttacaaa tattagcagc acatcaatat
     8341 ccaacaactg ctcaccttaa atttttaagt cgacaaatgc aaaatgattt agctaatttt
     8401 gctaaaagcg gtaaaatgcc ttggccgatg tatcataatg aacgtcgcta ttatcgcaca
     8461 tatcaataac gttatcgcat ctaaaaaagc acacctgaat ttgatagcag gcgtgctttt
     8521 catatgagaa ttatgatgat ttaaatgttt tagcataatg attatttttc aatgtgaaaa
     8581 caacgattaa tcctaatgcg ccaatgatgg cactcgtata cgtgatagct tgaacagaaa
     8641 catgtgtcat gaccaagcct ccaataatgc cgccaacacc aataccagcg tttaaactag
     8701 acatgttcca actcattact tggcttgtgt cgccttcaac atgttgaatc acaccgcttt
     8761 gcactgctgg attagtactc cattgcatga tattccaaat aaatagtcct gctaacaata
     8821 gacctgaacc aggtaagatt aaattcataa gtaacatcat aacgataaaa atagaaacag
     8881 aaatcattaa ccaacgctta cttgtaattt tatcggagaa tataccaccc aatgatgttc
     8941 caataacgcc agcgattcca tttactagaa gtgctaatga aacgaatgac atatcatgac
     9001 cattagataa aataagtgga tttataaaga cgaatgtcac tgagtttgca atcaatacta
     9061 aaaacgtaat aattaaatat tttgctactt cagcaggtct taatattttc gaagtaacat
     9121 gattttcatg agatggtgtc tcatgattca cagggcctcg ttgtatttcc tgatccttcg
     9181 gtaaatagat catcatcaag aagccaacaa taatactcac aataattaag aatagaaatg
     9241 tatagcgcca acctactaaa tcgccgataa ccgttccaat tggtacacca aaaacattgg
     9301 ccccactaaa ccctgtatag acaagtccaa tcattttacc acgattttta ggtgctgata
     9361 acatcgctgt aatagctaat acttttacga taattagtgc tgctgctgca gatgagataa
     9421 ttctaccaac tactaatatt gaaaaatttg gcgctacagc aataatgcca ttaccaatga
     9481 taaatataag taatgtccat aataatacag gccttgatga aaaacggttc gttaatttaa
     9541 ccagaatagg tccacatatc gcaaatgtta aagcgtacat tgtcactaat tgaccaacga
     9601 cagcttctga tacatgtaag tcctgactca tcaagttcat aattcctgca accatcattt
     9661 caaccattcc tacgataaaa atacttaaaa tgaatgtaaa tagtcgcatg attgacattt
     9721 taaaactcct ttcaaacaaa atttccgata ttcatttccc ttagaataac gatttattaa
     9781 aaaatgtgtg caacacaatt ttatatataa ccgttctatt atataatatt ttgctttaat
     9841 tttctgtgct taattggaaa attattgaaa ttgtttttca gtatttgaaa aattaacaca
     9901 agtatgtcat ttaataatta tgtataatta cttcagtttc aacaatgact cccaaaaagc
     9961 acgcacgctt tatatgtcag aacagataac tgactacaaa gtatgcgtgc ctttatgaat
    10021 attttcgctg tgttaatgaa tagcgatact atatgtgcgc gtcacttcat ttccaattaa
    10081 aactaatacc taaatctgat gcagtaaaat ctatcatgat tcactcttaa catcagtatt
    10141 tcgtgctact aattgatgtc ttgcattgaa aaattgacca tagcttaaat atgtcgcaat
    10201 caaagcagac ataatggtcg cagttgtatg aataaacacg actaacaatt gaaatttaat
    10261 cgcttgtaaa ggtggtacgc caccaataat taagcctgtc atcataccag gaatcgacac
    10321 aagcccatat gttttaaccg aatcaattgt tggcactata gctaaacgaa tactttcacg
    10381 tattgcacct ttagaagcca atttaggtgt agctgcaagt gataatttag attcaatatt
    10441 agtaccatct tgtacgaatg cacgatctaa attctggtaa gctaaattaa ttgcaatcaa
    10501 gccattatta gcaagcatgc cgccaatagg tataacttca ttggctgtaa attgaatggc
    10561 ccctgtcgca atagtacctg caagcggtaa tgccgttcca atgaagatag ctagaaatga
    10621 tatccaaaac acatgatgca tcactggtga tgctcgacta attgtattcc atgatgcatt
    10681 aataataatg accaatacac ataaaataag cagccattta tcgtttattt taaaaatata
    10741 atgcagcaaa aatcccaaaa tgattaattg cacaattgcg cgtagtgtcg caacaattaa
    10801 atctttaata atatgtaaac cttctttata tgaaatgata atcggaatga ctaaaagcaa
    10861 tgccgtcaat ccaagtgccg tattactcat gttgattcaa ctcctcaacc ttagaaattt
    10921 tgccatcaac aatcgttatg cgcttttgaa aatgtctcat actttgatca tcactatggg
    10981 taatccacat gatagcaaca tcctgttcca ctaacttaaa aatgatattt tctatctttt
    11041 ctttattatg aacgtctaat gcactggtcg attcatctaa taaaagaata tccggtgtat
    11101 acatcagttg gcgcgctata gcaattcttt gccgctcacc acccgacata ttttccactt
    11161 ccgaacttaa ttgataatgt cccaaaccga catctttaat taattgcttt gcacgttttc
    11221 tatcaaattt atcattacgt gcaagtgatg ggaatatcat gttatcttca atcgtttcac
    11281 caaacaagtc actttgctgc atcaaataac tgattcgttg acgcaattct tccgggtcat
    11341 aatcattata gggtttacct ttaaaataaa gttctccact agttggacta atcaaattac
    11401 atatttgctt ttgaaatgta cttttaccac tacctgatgg acctataatg gcaatactct
    11461 cgcctttatc tacttttaga ctgatatgat ctagtatgat tctatcgctc gctttataca
    11521 ctaaatcttt aatttctaac aacatttgca ccgctcctca tgtaatatat caggtatatt
    11581 attcccgttt ttttggtttt tagtgcaatt atatttaaga aaaaatcatt ttcttgtaat
    11641 atcaaaattt aggcataatt gattttcagc atttaaatta aaaacaaaaa atcctattaa
    11701 catggatact gtctaatgac taagaattaa aattagacaa ttgtttaaca acctaccata
    11761 ttaaaaggat tctttattat ttgtggattg ggttaccaca tgatattttt ataactttca
    11821 tcatttaatg aacggacaat ttcagtaaca agacggatag aattttcata atcatctaca
    11881 ttgagcactg aaacattaga atgcatgtat cgcagcgtaa caccgattgt catcgtggga
    11941 ataccttcat ttgcgacatg aatacttccc gcatctgtac ctccacctgg tgtcgtatcc
    12001 cattgtactt cgatgttatg ttccttagct acatctttaa tatgcttacg caaaccttgg
    12061 tgagcaatac ttgtagcatc catcataatg acaactggac caccgcctag tttactatcg
    12121 ctcgtttgac ctgacatacc tggggtatca taagcaatac ctacatcgac agctatcgcc
    12181 aagtctggtt taatcgtatt cgctgccact ttcgcaccac gcaaaccaac ttcttcttgc
    12241 actgtggcac cactgtataa gttaatgcca atattttcat cttttaaacg ttttaatacc
    12301 tcaatagcta atgcacagcc atagcgatta tcaaatgctt tcgcagttaa atatttatca
    12361 tttgcaagca cttcaaattc actatatggc gtaaccatat tgcctacttc aacgccagct
    12421 tcttcagctt cttccttgct actaacacca atatctataa acatattttt gatttccatt
    12481 ggctttttac gttcttcagg cgttaagaca tgtggcggtt tagaaccgat gatacctcta
    12541 atttctttgc ccgaatctgt tgtaatcgtt actttttgag atagcatgac ttgattccac
    12601 catccaccaa ctggcgtaaa tgaaataaaa ccatgtttat caatctttgt taccataaaa
    12661 ccaacttcat ccatatgacc agaaatcata attgagtatt gaccattctc agcatttttc
    12721 tttccaaaaa tgccacccaa gttatcttca ataatttgat cactgacagg ctctatatag
    12781 ttacgcattg cttctttaac ttgcatttca taaccagcaa tgccgtttac atctgttaaa
    12841 tgtttaagta atttgactga ctgattcatg ttattcatcc tcctgtattt caactttaaa
    12901 ctattgtatc actaactttc agaaaaacgt tccctaccgc tttatcattt tcaggcttaa
    12961 atatttttaa aattttgtga ggaatccgct taaaaaaact catatttcta aaatttaata
    13021 ttccgaaaaa gtcacaattt aaaatccctt attataatac gttattcttt tattgttact
    13081 aatttcaatc tgtttagtct tgcgctatta taaaattaaa aagtgtagag ataacaacgc
    13141 attaacgtcg ctattttccc tacacttaaa tactagtttc tttaattaac ttatcattaa
    13201 agttattcag atattgaata ttcattaatc ataatgtgac catgccgata gtattaatac
    13261 ttctttattt cgatcatcta cggtatagac gaccctgtgc tgatggttaa ttcttcttga
    13321 atatcgctct aaatatttag gctctgattt ttcaaaagat tgtgttattt tatacggatc
    13381 attttttaaa gtctcaacaa tttctaaaaa tgacttcttt aaataagaat gttttatttt
    13441 ctttaaatct gatttcgctg aatttttaat cttaaccgtg taattgctca ttaaagatta
    13501 tcccaatcaa tatcatctat atttgttgta ccactattat ctttttctct ttctcttact
    13561 ttgtccattg taccagtaga ttcaagatat attgtctctt gtatactttt ccaatcttct
    13621 aaacctataa tcacagcatt attttcggca ttattgccac taatataaat tggttcgtga
    13681 ttattattaa cattttttag taattgataa aaatcttttc tcgcttctgt agggctagtg
    13741 ataatcatat aaaccctcct ttgttttctg ttacgctaat tatagcgtac gcacttgagt
    13801 acgtcaatat taccaacgag ttcatcgtat tcttaatacc tatatgctat cgtacacaag
    13861 cttttgttct tttcttccaa cataccgtct ataatgctca aaaagaaatc agcagtggaa
    13921 cacactacgt tccctaccgc tttatcattt tcaggcttaa atatttttaa aattttgtga
    13981 ggaatccgct taaaaaaact catatcttca aaaaaagtga caaaatttaa atttttgaat
    14041 aacatcattt tttagtaaat gttataattt ctttataata caaatatcga aagaaggcga
    14101 ctgactttga aaaaattatg ttcattaatt gtagtagcat tagtttgtat cattgcatta
    14161 tcagcttgtg gtaaagaaca aaccaaaaca tatgaaggtg atgttagtgg taaacatgta
    14221 ctaacttcta tcacatataa agatgataaa gttttaaaac agtctacaat caacacaatt
    14281 aaatatgacg atctaggtat ggataaagac gaagccaaaa agttattcgc caaatctgaa
    14341 agtattttca aagaccttaa aggcgtaaaa tacaaagtag actataaaga taaaaaagca
    14401 attgaacact tagacataga ttacacagaa gttgacatga aaaaattaaa taaacgtctt
    14461 ggtgtttcaa ctaaagaaaa taaagatatt agttttgaaa aacttgaaaa gcaattaaag
    14521 cacagaggct taaaagaaaa agataaaatg gacgacaaat agtttataac ataaaaatgc
    14581 cctcagataa gactaaggtt acaaacctta actcatattc tgagggcttt aatatttgaa
    14641 attcttgtgt gaccagcatc cactactaat ataaaattat ttgcagtaac actaaaatcc
    14701 gctgctttca ttttctcgaa atagataaat caactaatca attttaattt ataatcatgt
    14761 atcgtttgta actcaccatc gacttttcga tatacaatat gatcagcagt aatttctgta
    14821 ggactggata cgccaacagc tgctgcaata ttgaataagc cttcatgcaa acttgttaca
    14881 tagtttgtga cacgatattg cttttctcca acaatcaatg ctttttcttt tttagcatct
    14941 gttgttgcaa caccaactgg acacgtattc atgtgacatt gttgactcat tatacaaccg
    15001 acactaatca tcatcccacg tgcgatattt acaaaatctg cacctaaacc tagtgcaatc
    15061 gcaattttat ctggtgtcac taacttacca gatgccgcca atttcacttt atctcgaata
    15121 ccatattttt ctaacatgcc agacacaata ggtagagctg taaatagcgg taagccaaca
    15181 ccatcttgta attcttggaa tgttgcacca gtaccacctt caccaccatc aatcgtaata
    15241 aagcttggat acttatctag ttccaccatc gtacgtacaa gtgtttcaat ttctgaaact
    15301 ttgcttacta caattttgaa tcctactggt ttttgaccta attgctgcaa ctgatcgacg
    15361 aaacgaatca aatcttcagc attatgaata aattcgtaac ggttaggtga attgattgtt
    15421 ttataaggtt caacatttcg gattttagca atttcttcgt ttaccttttc agcttccata
    15481 tgaccaccac gagtcttagc accttgtgcc aacttcagct caaatgcgcg tacgttagat
    15541 aactgtgcaa cctctttaaa taaaccttca ctaaaattac cttctttatc acgaacacca
    15601 aataaaccgg gaccaatttg gaaaatgata tccccattac cttttaaatg atattctgat
    15661 aagccacctt cacctgtatt catccaagtg cccgctttag ctagaccttt agataaagct
    15721 gtaatagcat tttttcctaa agcgccataa ctcataccag attgtcctac gatacgtttt
    15781 aaaataaatg gatgttttaa acgttcacct aattttattg catggtcatc acttaagtaa
    15841 tacggatcaa tctttgtcgg tacacgatat tcttcacgac taaataaacg ctcattcgcg
    15901 attttataaa tgaatgttga taacaatgtt gtattatcta ctgaaatctc attacgttgc
    15961 atcggaaaca ttgtgttctg tatgtaaaag ccgtcttgat aatctttagt agtaccgaag
    16021 ctggtcatac gagagttata ttttccagcc aaaacgatat ttttatagtc attacgtgaa
    16081 aaaggtttcc cttcattatc cccagaaaat aaatactgac gtaattccgg tcccattttt
    16141 tctgaaatat atctaatacg tgctagtaaa ggataattcc ttaatacact atgttgtgat
    16201 tgtcttttat ctttaattaa ccaaataagc ccgataacaa taaccgtaag caggaagcct
    16261 acaacgataa tgttaactat aaattgcatg actgtaagaa acgtcattac aatccctccc
    16321 ccaaaaaatt tcaattcaat atttatgata caccttacaa aataaaacac aatggaagcg
    16381 tttcatttta taaaacaatt ttatgatatg tttttcattt taaattttaa tgtataaaac
    16441 atacaataca aagtaatatg tgctaaagta tctatataat acaactattt aagaggtata
    16501 ctatgtcaaa tacaaataaa cattacatag aagaagaata cgctactgaa caatctcgtt
    16561 ttttcaaacg tgatattgga tttattttcc tttatatatt tttggttaac atcttgccaa
    16621 tcattatcgt ctttttaact tttgggttag tagctgtatt cactcaacaa gccccatggg
    16681 gtttctcttt tcctatttca atgaacatta gctatttact cgcagttatt atttcactag
    16741 ctatttttcg tcggatgcat gaagatgcgt ttattccaat gatacgttcg caaatcgttg
    16801 ctagtgctaa atataaatgg catattctta tatcatttac actaagtgtc gtactctttt
    16861 tacttttaaa tcatttcaat attttcgcac taaatgtaga tgcctttact gtctttgaca
    16921 tttacccgct cattaaaggg tatggttcca tcagtttaat agctatattt tcgttaacca
    16981 ttatattaaa tactttgatt tgtcaactta ttttccgtca tattttaatt caagaattaa
    17041 gcagattaat ccctttaaag attacaatag tattatccgt tattcttgaa acactgtact
    17101 attatccata cattaataca tggtgggaat tcattccagc attgatacta gcaagtagtg
    17161 cgacttactt atatttaaaa tcataccgta attttatggt gagctatttt tatcaattag
    17221 cagttatact cgttttgcat attttcatgt aaaatatttc agagtattat ccattatcca
    17281 tacacatcaa aaagcacact taaaagtcag tatcgcaact cacttctaag cgtgctttat
    17341 ttagatatac tgtcgttcta aaaacgagcc atatagagag cctgagacat aaatcaatgt
    17401 tcaatgctct acaaagttat attggcagta gttgactgaa cgaaaatgcg cttgtaacaa
    17461 gcttttttca attctagtca accttgccgg ggtgggacga cgaaataaat tttgcgaaaa
    17521 tatcatttct gtcccactcc catgaatcgc catgctcaat atacctttta aaatttaaat
    17581 attcattaac ttaatatgct ttaaactttt tcattaaaga ctactcgctg ggcgtggtgt
    17641 tggttttttc aataaactca taatcattat tacaatgact aacataccac aaattagata
    17701 catccattta aatcctacta aatcggaaat aggtcccatc aatgcgccac ctaacgatat
    17761 acctaagtct gcacaggcaa taaataaacc taacaacata ttacgaccta cttttggtaa
    17821 gacgaagctt aagtatgatg ttaatgttgg gtacaccatt gcttgtgtca ttcctattaa
    17881 tattgcacta ccatagaaaa taattgcacc tacttgcgga ccaaatgcca ctacaaatga
    17941 cgcgatcact aataatgata gtacagatac catatattta ggatgccaca taccatctga
    18001 cggaatgtat ttccttaagt aaaatcttgc cacaacaact gcgatggcct gtattgtcaa
    18061 aaagattccc gcattcgcga atcctaaact cactgtgtat aacggtacaa atgtactaac
    18121 tgcaccaaat acaatcgatg caacaatcat gataatacca ctgttcaaca actctttatt
    18181 tttgaaaaat tgcgcaaaaa cagttacagc gttaaacggc attttttcaa tcttatctga
    18241 cgtatcgggt tcctgttcag caaaggtcac gcgatagcca aagaatgttg ttgttaatgc
    18301 gataaagata atgacaattg caaatagtga aatattattt gcattccaaa tacctacggc
    18361 aactaatggt ccaattaagt ttggaatcgt tgaaaatagc gagtacaatg atacaccttc
    18421 agaacgatgt tcctctggta atgcatcaat aatacctaac tgtaaagaca tagaaaagaa
    18481 tgccgtacac acaccttgca taacacgtgc tacgaaataa ccttctaatc cagtaaaacc
    18541 ataaataatt aaagctatgg cattgatgat caaaataatt cttaatactt taatgggacc
    18601 gacacgagca ataatttgtc ctgcccatgg tcgaaacacc attgctgtta acatgtatgc
    18661 ccccataacg ataccgataa ccgtattcgt tgcgcctaaa tcatgccctc gtaacggtat
    18721 aaatacgttt aagattgcat tggcactaaa gaacatcaat gttaatatat ataatctcaa
    18781 aaagggccaa gccattgcac ctttcattat ctcactcctt agaatgatag ttgtttttct
    18841 attaattctc ttgtataagc attgtcactt ttatgcaatg cgcttgtcgg aatttgttct
    18901 tctatttttc cgtttttaaa aataattaat tgatcacata aatacgtggc agcttgaata
    18961 tcatgtgtga taaaaatata actcaactga cgcgtttcac gtaaatgaat caataaatct
    19021 aatatttgtg tttgaattga catgtcgagt gaactaatgg cttcatcaaa caaaatatat
    19081 ttagggttaa tacatattgc acgcgcaatc gcaacacgtt gcgcctctcc acctgataac
    19141 atattaggat atttatccat gtatgcctta gatagaccga cttcttccaa caatgtaatt
    19201 gcttgaactt ccataacatc tttaggttgt ccatcacatt gacacatcac ttcaaataag
    19261 atttctctaa cagtctgaaa tggatgtaat gatgacgtat aatcttgaaa tacagcacca
    19321 atttgatgac gtctcacttt cttcttatgc atcggttgat catttaaggt tacacaacct
    19381 ttatccggtt tctcaatacc taatatcata cgactcaatg tcgatttacc gctaccactt
    19441 tctccgataa tcgcaatcgt cgcaccgatt ggacactcaa atgacacacc tttcacgata
    19501 ggtgttcgac gacgcttaaa aacatgtgcg ctttgatatg acttttcaac atctttaatt
    19561 ttaatcatgt acatcacccc tcatcacatg tttaaaatga tcattaatct tcttcttcgt
    19621 tgataataaa tacttcgtat aaacatgttc tggatgatgc aagactgatt cacgtgtccc
    19681 ttgttcaata agctgaccat ttttcatcac aacaacacgg tctgcaatct tgttaataac
    19741 cgttaaatca tgtgaaatga aaatcatcgc acagtcaaag tgttttttaa tatctataaa
    19801 tgcttccagt acatcatatt gtgtaattgt atctaaagcc gttgtcggct catcagcaat
    19861 gattaacttt ggtttcaaag ctaacgctaa agcaatcatc aatcgctgta acattcctcc
    19921 tgataacatg taagggtatg attttaatat acgtttagaa tctttcaaac ttaaataatc
    19981 catatattca atcaatgtct tttcaatttc ttgtgtagac attgacgtat gtactttcat
    20041 agtctcaaac atttgtttac cgacagtagt tgatgggtca aaggcacgac taccttgttg
    20101 catgaccatc gcaatgtctt taccacggta ctttttcaat tgcgattcag ataatgacaa
    20161 cattgatgta ccatcaaaga taatttcacc tgtcaccccg agtcgttcga gattcaaacc
    20221 aataatcgat ttacaagtga ttgatttacc gctaccactt tctccaataa cgcctaaagt
    20281 ttcaccctta gttaatgtaa aattcacatc actcacgagt ggttgatctg tccaggtatc
    20341 tgtaatcgtc aaatgtttaa ctgttaacaa tgtcatgatt gcaccactcc ttttttcaca
    20401 gaacgaagtt tatctttaga agagatgcgg ggatcaatag caatttgtaa agcatcggat
    20461 aagaagttaa atgccatcac tataatcact atggcaatac ctggcgcaaa catcatttca
    20521 ggatgtgtaa acatcacttt tctagcttcg ttaagcatca tgccccactc tgcagtaggc
    20581 gctttgacac ctaatcctaa aaatgaaaag ccagatattt gcaagatcat tgaacacatc
    20641 gaactactag agatgatagc aatatctgct aatgttaacg gcataatatg tttgtgaata
    20701 attttcatat cattcatacc gattgtttta gcaaatctta catggtcaga agcagtgtac
    20761 tgcataacac ttgtacgtat aacacgacag aaccatgccc aacgcgtcaa aataaatgcc
    20821 atgataatat tttcggcacc cataccaaac aatgcaatta atgctaacgt tacaacataa
    20881 cttgggaatg ccaacataac atcacacgca cgcatgatta aggcgtcaac aaacccttgg
    20941 aaatatcctg ataagaatcc taaaatagat ccaataagta cagaaacaaa tagcgcaaca
    21001 aagacatata acaaacttgg tctaatcgca taaattaacc tagttaaaat atctctacct
    21061 aaatggtcag tacctagtag atgttgaaaa ctcatgccag caaatttgtt tgctgtatcg
    21121 atatggttag gatcataaaa tgtcacaagt ggtgctgcta atcctaaaaa gacatataat
    21181 acaataatgc ctaaagcaat tactgcacct ttatcttgta ataatcgttt taaaattatc
    21241 atcgtgcgcc ctcccttaat cttggattta atagcgcatt aatgatatct gctaatgtat
    21301 taaatacaat aaataatacc gctacaatta atacatatgc ttgaataact ggaaaatcgt
    21361 gttcaagtat tgcttttaaa cttaattgac ctagtccagg ccatgcaaag atatactcga
    21421 taacaactag tccacccatt atcattggta tagacataca aaagattgat accgcaactt
    21481 gtaaagcatt acgcaacaca tgcaacatta atgtgataga tttcacaccg cttgctctta
    21541 aataaagtac ataatcttca tttaattgtt ccaccatcga gcgtctaaca tttctaaagt
    21601 aaatagcagc ataggcaatc gtaataacga tcactggcaa tatgtaactt tctggacctg
    21661 ttaatccaga agtcggcaat atgtttaact tcactgaaac gtaaataata agtattgaag
    21721 ctatccaata tgatggtaat gcagttagaa agaaagccac tgaacgtatc gcacgatcag
    21781 tgaactttcc tctttttaat gcactaacta cacctaaaat aattgatgta atcatcacca
    21841 taacacttga aattattgtt aatttcaatg tattcataaa tgctggacca atgcgttcag
    21901 caacaggatc acctgtaatg tagcttgtac caaaattaaa ttgcatcgct tcaagtaacc
    21961 aatttttata ttgaattaat aatggatcat tgaaaccgta cttctcattc gtttctgcaa
    22021 tcaactctgg tgttacattt ggcgtccctt gtgcatgtaa aattgtcaca gctggatttt
    22081 catttgtaat atacgtcaat agaaatgtca taaaacttac tacaatcact aatggaaaca
    22141 tgagcgcaat acgttttaag ataaatttga acatctaatt gctcctttat ttatactgca
    22201 tttcattgaa tggtaattca tactgtgatt gtgtgaatga tactttttct aaatctttcg
    22261 gcgcaacaac tgtcatacta ccgtgtgaaa tagggataaa gataccttca tcatcaattt
    22321 gtttcaaaat gtttttataa gcgcctgaac gctctttacc gttttggatt ttaaatgcgt
    22381 catcaatgct gttgtatatt ttatctttgt tctcaatgcc tgatgttgca ctttcataac
    22441 catttttcgc tttaaatgct gcaatagtac tttgtggatc gtacaataat ccccaagttt
    22501 ggttgaacat taagtcataa tcaccagaag tacgacgttc agcaatttta tctgatgttt
    22561 cgccattgat gtttaactta atacccattt tcttaaattc tgcttgtaag tattctgctt
    22621 gttctttttg acttgaagaa cctttgtcat agtacattgc catttcaagg tttttaccat
    22681 ctttttgacg aacatcgctg tctttaactt tcttccaacc agcttcatct aataatgatt
    22741 ctgctttttt aaggtcatac ttacgtgttg gcatatcgaa attaatatct gttacatttt
    22801 tcgcaaataa ttgagttgct ggtttttctt gaccatctaa aatttctttg gcaattttat
    22861 ctctgtttac catatgacca atcgcttgtc tgactgtttt gtcactcaca gcgttatctt
    22921 ttttaccaga attgacaact aacattttcg tattcatagg ttgactacgc ttaacttgat
    22981 agtcacctgt atctttcaat tgttttaaag agtctttgtc taagctatcc gtacctctat
    23041 catctgtgaa ggcaaagttc gtttcacctt ttttcattga taggaatgct gtttcaccag
    23101 caggcattac ttttgcttgt actttgttaa gtttagactt ttcgccccag tattgatcat
    23161 ttttgttaaa gtctgcagac tcatcttttt tatgttcacc taatttaaat ggaccagtac
    23221 catcgaactt tttaacgcca tcttttgttg taccgttttt aaagtctttt ggagacacaa
    23281 atacatatgg acgaggcatc gctaattcag ccaatgcagg ttgatatgct tctttcaaat
    23341 tcaattcaac cgtgtactta tctttaactt taacattgtc aattaatgtt gaaatcttta
    23401 accaagaatg caatttttta ttttgttgaa ccgcatcaat atttttctta actgcgtcag
    23461 catcaaatgg tgtaccatca tggaatttaa cgtcatctct caaatggaat gtatatgtct
    23521 tcccatcttc agacacatcc cattttttag ctagtaaagg cttaattcca tctttcgtgt
    23581 tacgtacaag cggctcgtat atcatacttt cagcagacat cgatccaccg taaacatgcg
    23641 gattcatatc accgatatct ttaaccgtcg tatacgttaa ttgcttgttt tcttttttct
    23701 cctctaaacc tttattaccg ccacaaccag ttaaaattag ccctgatgca agtaacattg
    23761 cactcatttt agttagtttt ctcatttgct tttcctcttt ctaaattgat aagttgcatt
    23821 acttaatcaa atcgtaatga ttatttttct caattatttg cttattttta tgtaaatgtc
    23881 aaacataatt caataatttt tttctattat aaaacttcaa acccttattg tgaaagcgtt
    23941 ctattgattt ccaaaaattt tgtgacgagt gctttatctc cttcaaatag atttgtatta
    24001 aattgagaag ataagtgttg atcttgatgc atatccttgt acgcctggca acttgcttca
    24061 tagcgagtta ggaactgatc aatcattggc gttttgatac ctagcatttt cccaatatgc
    24121 tgaatcatcg ccgttctgta ataatcttca cttggcattc ttggaatttg aacaacatcc
    24181 tgttcatttt tatagacttg cttaaatggt acagctgaaa aatcaaagta atgtccgttt
    24241 tcgtctggct gtgaaaatgg atcaatgagg attgcggtat atcttacata aagcagatat
    24301 tcttgtaaga tatctggcaa gatttcgaaa tgctcaatat caccttcatc caaagtttca
    24361 ggacgtactg gataattttc cttcaccata aattgaagca ggttgactga cggcactcta
    24421 aatgcttgta aaatagccat catttccttc cacattaaac gcatttcacg gattagtgtc
    24481 atcgttatcg gtccttcagg aaataactta tacacataaa ccggtacatc cgtcccttcg
    24541 aaaatggctt tcaatgaaaa gtcattcata aatagtggtg ggtgcacata aagcgaactg
    24601 tttcgcgttt cagcgtgcag tggcgattca acgacttcta attgaatttt caattgctca
    24661 gctaaagcag agattcgttg acacattgtt gagtttgaat gtgtcgatcc catgtacaat
    24721 ttctttttta cacctgttgt caacacatga ttaggcgctt ctttatcaac aatacgtgta
    24781 tcgccaagat aagttgagaa tgaaatcact tcgatatctt tattaaattt agacataaat
    24841 tgttcgacaa tcatttgcga accaaatgtc ggtgatatta aaatgacatg tttgacactt
    24901 tgcaaagttt ctaacgacaa ttgctgtagt gtgtcataat aagcatctgc tgtgcatgcc
    24961 atgacaaccg tttcgtattc accattaacg tttttaacgt ctttatacaa acgattaatt
    25021 tcaaacttac cttccagatg ttgatgcgcc tcgttttgta ttttgacttc aaattgtttc
    25081 tcttttttat acgcttgata taagcgtttt gatttttctg atgttgaggc acgtccaacc
    25141 atatcaatct cataatctga ttttaaatag caaatattcg ctaattggat tgcgaccgga
    25201 ccagtgccta tcattaataa tttagacatc cgtaatacct acctttatag ctgctttttt
    25261 ataaagtgct atatcaaaaa tttgctgtgg tctcatatgt ttattcacac attgccactt
    25321 atcttccgct gtttcttgtg acggataatt aaatattgct ttgatgccat caccaaagcg
    25381 cattgcaact acgacatttt catttgttaa atcatataat tcttctaaaa tgctgtactt
    25441 taaaggaatt gtcgagctga atatgatatg cgtcacatct ttgatatctt taagttcaga
    25501 taccttttga tccgtaattg ttatatcttc atttggtgct aagacgttaa cgattctgcg
    25561 ccctaggtca acggcttgtg gatcaatatc aataccgata actgaagcac ctgtttcttt
    25621 tgctacttga attaacgtca ttggatatgc acctgaacca actaacaata atttgtcatt
    25681 gctagctata ctacattgtc caaactcttc atcaatacaa tgttctatat tgtcaaagta
    25741 acctgtggta gacgcctgtc cgtctaatag acgtctcgct ctaatgactt ccacttgttt
    25801 aacacactgc gctgtaatat attgcagtga ttttattaat tgtgcttttt cttcaacgtc
    25861 acgccaagca ttaaattgtt gttcataaat aggatttaaa ataaattcac tatagtcatc
    25921 catcaatgtt tctaatgctt cgatatattg atcgtcttgt aatacacgct cgtaatgcgc
    25981 ttcaaacttt tctaaatatt gttgtaatat caatttgatt tcattattaa agttattcat
    26041 tgtaggctcc ttattctata tatgcttttc ctgtagctac agttgtaacc tgtcctttaa
    26101 ttgaagtttg atatcccaat tgatgacatc gctttgatgt cactaaaata ctgccccctg
    26161 gctgatgtac tgtaaaatct ttgcatgcgt cattacgttg ataattatta aaaaccccaa
    26221 ttgatgctgt accagaacca cagctatttt cccaaattaa actttgaatt tctggtatat
    26281 agattaatgg ctgtaaaaat tgacgttgtt catcaaaaag catcatacct actgttttat
    26341 atttgtgact ccattgttgc tcacgcacaa acgcttcaac caaatgttga atttctgttg
    26401 ttacttgttt aactggaatc acataatgta catatgtttc ataaataatt tctattgctt
    26461 tccatgaatg attacccata ttaattgttg ttggcacaac acgatgggct tgtggcattt
    26521 gaacttcata gtattggcaa tcatgaattg cgcattgcac taaatccgaa cagccagaca
    26581 ccttcacctt aaactgttgg tctttaagca aatgactttc ttgcaaatga tgtatatatg
    26641 acatcgtcgc attaccgcaa aattcattac cgctcataac taagtgaaaa tcattgccat
    26701 catcattttg tgttgattct ataaagccta cctgttcaca acatacatgt gttgcggcca
    26761 tcaactgatt ggcgatagat gcatattcac tagcatcatg ttttgaatga acaagtatcg
    26821 tcatattccc cgaaggatta tacttagaaa attctataac ctgccgattc atacgacact
    26881 cctttagatg tatttttaga gcctctgtaa ttttaattac tagaggctct tatgcagttg
    26941 ttgcgaagca aacaactgta ttcctctaaa cctgtaattt tttagaagat gaacctaata
    27001 gctctatcta gtaggtaagt catgctaatt cgcaaaagcg aaaaccatgg ctcttatgca
    27061 gttgttgcga agcaagcaac tgtattcctc taaacctgta atttttaata gaagatgaac
    27121 ctaatagttt cccatctatt aggtaagtca tgctaacccg caaagtggaa agcatgactt
    27181 agtaatcggt tgttgtatca acatgcaacc gtaaacctta tttaaagttg cttcttgtgg
    27241 ctctacaagc gaatgcagtt gtccgttaac tacattcact tagatgcttt tactgttttc
    27301 acctcaacta aaacttcaaa aaagttgcag aaaacaaatc gtaactatta cgatttgatt
    27361 atatactata ttagttgaga atcattgtca ataaaggttg taaatgaatg cacatttata
    27421 ttttgataat ccttccataa acatcgtatt ctcagcacat tcaagtcttt ttgaaaattt
    27481 ttatcaataa ttgaatagaa gttccgttct cgtcagttca aaattttacg taagttgtct
    27541 ctgcgtattg ctttagtgag aataacccac acttgtggaa acgtcctttt gttaatctac
    27601 aatggatccc cttgtctttc gctaacattt cctatcacca atcctgtaac gaattttcat
    27661 cgtcaagttg ttgcccatgc aatgcgcacc aaaaaaagct ttgtacaatt tcgtacaaag
    27721 ctttaaaaat tcaaataaaa atatcattat tttaatgaac tagccatatc agattttatt
    27781 tcgtttgcta atacttgtaa ttcagggaaa taattattag caaagccaat tattatttta
    27841 ttttttacaa acgtgttaat atctcgatct tctggaatcg tattttctat taaattcaaa
    27901 agctcactat gacttatgtt ctctatactt actgtattta aatattttaa aatcgcaaga
    27961 tacaattcat caaatacttc atgttcaaaa tcaaagtcca ataataataa actaacggaa
    28021 ttagtttcta actgattatc tccaatttta tctgcaatct ctcttatagc ttgatatagc
    28081 aatctaaaac tttctttttc ttttaacata aaattctcct taatttatta gaatagacaa
    28141 aatacagctt attttaaaga tactccaaaa gtttgaactc gagattctga taaattagat
    28201 gcacgaccta caatacgtta aaacaagctt taagcaatac acttcaaatg aacaatatgt
    28261 gattttcaaa gattaattaa tctcggcttt aaatgtcttt cattgcgaca atatgcatac
    28321 attgcctact tcatgccaaa ttcttttcca ctggatatcg attttaaata attattccaa
    28381 tgtccgcttc cacgaatcat cactcaacat ctcgccaggc catgtgtaag ccataacacc
    28441 accgtcaata cgaattgttt cacctgtaat aaatgaactc tcatcagatg ctaaaaagac
    28501 aacgagcttg cctacttctt ctggtttacc taaacgtccc agcggtgtca tccatttctg
    28561 attttctctg aatgctttgc cttccttatc ttcactcgta cctgtcagtt tatctactaa
    28621 cggggtttca atcgtacctg gtgcaatcgc attagctcga atgccatctc taccatattc
    28681 gattgcaatg gatttcgtaa aattaatcac tgcccctttc gcagcattat atcctgagcg
    28741 atacaaatct gctgcctgtc ctgaaaatga agatgtgtta acaatagagc cacctttagt
    28801 catcattaaa ggtaacatca ttttcgtcat caaaaatgtt ccacgcatat ctacattcat
    28861 aatcttgtca tatacatctg ttggatactc atgaattcta ccagccgcat tatccacacc
    28921 ggcattatta aataacacat caacatgccc aatctgttct ctaatttctg atgcaaaatt
    28981 gtcaatttgt tgttcgcttg ctatatccac aatataagcc tttgctttgt caccattact
    29041 tttaatttta tcgacagtct ccgataccgc ttcagctatg tctaccgcca atacatacgc
    29101 accttcttga gctaaagcga ttgcagaagc ttgtccgata cctgtacttg ctcctgttac
    29161 gactgcaact ttattttcca aacgtttcat tcagtcactc ctatacaaca tatcttaaac
    29221 ttacagtcct tattttccac tgttcaatgc ttcataaacc taatagataa gtctccaggc
    29281 agtcttatct actctctgct atacaatttc tattgaataa ctcaaaatat ttaacaatat
    29341 tagatttttc attttctgaa aattgttact tttgagagta ttttttgtta cactttaaat
    29401 atttaacaaa ggggggaatc attgatgaca tcaaaacatc aacaaaaggg gtctatcgtc
    29461 aatcgtttct tgaatagtgt tgaaaaaatt ggaaataaat tgccagatcc tagcgtttta
    29521 ttctttttaa tgtgtgtggg cttagccatt atgacgtggt ttatctcatt atttaatgta
    29581 tctgttaagc atccaggtac gcatcaaacc atttatatta aaaatataat tagccatgat
    29641 ggatttacga tgataatgaa tgatacgatt aaaaatttct cagagttccc agcattaggc
    29701 ttagtactag cagtgatgat tggtattggt gttgcagaga aaacaggata cttcgacaag
    29761 ttaatgattt ctgttgtgaa tcgcgcacca cgtttcttaa ttttaccgac tattatatta
    29821 attggtattt taggtagtac agccggcgat gctgcgacaa ttatcttgcc gccgctcgca
    29881 gcgatgcttt ttattaaaat tggctatcat cctatcgctg gactaacgat ggcatatgct
    29941 tccgctgtag gtggctttgc cgcaaatata gttgttggta tgcaagatgc tttggtctat
    30001 tcatttacag aaccagcaac acgtatcgtt tcagattcta ttaaaacaaa cgttgctatg
    30061 aactggtact ttatcgcagc gagtgtcgtt gtattacttc ctacgatatt gctagttaca
    30121 actaaactga ttattcctag gttaggtcaa tatgatgatc gtttaatgca tgatgatcat
    30181 gaagaaacgt cttcacatat tactgacaaa gaagctcatg ctttaaaatg ggcaaatatc
    30241 agtttcatag tcacaattat tttattaatt attacagcca ttcctgaaca tagcttttta
    30301 agaaatgcta aaacaggcag cttactagac gatgcgccat taattaacgg tgtcggatta
    30361 attattcttg ttgtattttt agtacctgga ttagtttatg ggattttaag caaagaaatt
    30421 aaaaatacaa aagacttagg taaaatgttc ggcgatgctg taggatcaat gggaacattt
    30481 atcgttattg tgttttttgc agcacagcta cttgcgtatt taaagtggag taatttagga
    30541 attatcgctg ctgttaaagg tgccaaatta ttagaacatc aaaacggcat tgtattgata
    30601 ctaggtatta ttgtactgag tgcaatggtc aacatgttaa tcggaagcgc atcggctaaa
    30661 tggggtattt taggaccgat attcgtacca atgttaatcc ttatcggctt tcatcccgca
    30721 ttcacacaag tcatttaccg tgtaggagat tcaattacca atccaattac accgatgatg
    30781 ccgtacttac ctttattatt aacttatgca caaaaatacg ataaacgcat gaaactcgga
    30841 gccttacttt ctagtttaat gccgtattca atcgcgctaa gtattgtgtg gacagtattt
    30901 gtcattattt ggttcctatt aggtatccct gttggaccag gtggaccgat attcgttaaa
    30961 taacagtatt caaagggata aattaaaatg ccactataca agatttgacc tgtatagtgg
    31021 catgatttgt tatgatatag cgttcgagat gaaactctgt atttatatga agaattatag
    31081 tttatcacta ataatattta gcctgttcca accgttaatt tggtttacaa caaagacaag
    31141 atcaataatt tgttcttcat cataaaatga ttttagccgg tcaataattt caaacttctt
    31201 aaagtctttg attgaattta acatttctgc aaattcaagc gtcacttttt cttgatcagt
    31261 aaataaatct aaatgtctaa aactcaagac ttcatcaatt ttcatttgtg ttacacccaa
    31321 ttcctttaat tcttttttat ggatatcaac acaataatga caaccgttaa tttttgaaat
    31381 taataactta atcaatgcac taagttcatc attaacgggt gattgatgaa taaacttttc
    31441 accttggctc attacagaca ttaactctcc attttgtttc ttgtaattat acggcatttg
    31501 caatctctcc tttacattgt ttaataccaa tccagtgata ctacacttcg aacaatattg
    31561 gatagaattt gaaattctgt ataac
//
LOCUS       DAMZKC010000012        31462 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.012, whole genome shotgun sequence.
ACCESSION   DAMZKC010000012 DAMZKC010000000
VERSION     DAMZKC010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 31462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31462
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.012"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(44..136)
                     /locus_tag="POB55_000399"
     CDS             complement(44..136)
                     /locus_tag="POB55_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499395.1"
                     /translation="MLKSKFHSPLSTVDISFFESSYIFSYQDTL"
     gene            complement(474..878)
                     /locus_tag="POB55_000400"
     CDS             complement(474..878)
                     /locus_tag="POB55_000400"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HDI6499396.1"
                     /translation="MNTLNLDIVTPNGSVYNRDNVELVVMQTTAGEIGVMSGHIPTVA
                     ALKTGFVKVKFHDGTEYIAVSDGFVEVRKDKVSIIVQTAETAREIDVERAKLAKARAE
                     SHLENDDDNTDIHRAERALERANNRLRVAELK"
     gene            complement(898..2310)
                     /gene="atpD"
                     /locus_tag="POB55_000401"
     CDS             complement(898..2310)
                     /gene="atpD"
                     /locus_tag="POB55_000401"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017636767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HDI6499397.1"
                     /translation="MGIGRVTQVMGPVIDVRFEHNEVPKINNALVIDVPKEEGTIQLT
                     LEVALQLGDDVVRTIAMDSTDGVQRGMDVKDTGKEISVPVGDETLGRVFNVLGETIDL
                     KEEISDSVRRDPIHRQAPAFDELSTEVQILETGIKVVDLLAPYIKGGKIGLFGGAGVG
                     KTVLIQELINNIAQEHGGISVFAGVGERTREGNDLYFEMSDSGVIKKTAMVFGQMNEP
                     PGARMRVALSGLTMAEYFRDEQGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTTKGSVTSIQAVFVPADDYTDPAPATAFAHLDATTNLERKLTE
                     MGIYPAVDPLASTSRALEPSIVGQEHYEVARDVQSTLQKYRELQDIIAILGMDELSDE
                     DKQTVERARRIQFFLSQNFHVAEQFTGQKGSYVPVKTTVANFKDILDGKYDHIPEDAF
                     RLVGSMDDVIAKAKDMGVEV"
     gene            complement(2332..3198)
                     /locus_tag="POB55_000402"
     CDS             complement(2332..3198)
                     /locus_tag="POB55_000402"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HDI6499398.1"
                     /translation="MASLKEIDTRIKSTKKMKQITKAMNMVSSSKLRRAEKNTKQFTP
                     YMDKMQDAITAVAGASSNTNHPMLRPRKITRSGYLVITSDKGLAGAYSANVLKKLITD
                     IEAKHQDSSEYSIVVLGQQGVDFLKNRGYDIEYSQVDVPDQPSFKSVQALANHAIDLY
                     SEEEIDELNIYYSHYVSVLENKPTSRQVLPLSQEDSSKGHGHLSSYEFEPDKESILSV
                     ILPQYVESLIYGTILDAKASEHATRMTAMKNATDNATELIDDLSLEYNRARQAEITQQ
                     ITEIVGGSAALE"
     gene            complement(3229..4737)
                     /locus_tag="POB55_000403"
     CDS             complement(3229..4737)
                     /locus_tag="POB55_000403"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDI6499399.1"
                     /translation="MAIKAEEISALLRSQIENYESEMSVTDVGTVLQIGDGIALIHGL
                     NDVMAGELVEFHNGVLGLAQNLEESNVGVVILGPYTGITEGDEVKRTGRIMEVPVGEE
                     LIGRVVNPLGQPIDGQGPINTTKTRPVEKKATGVMDRKSVDEPLQTGIKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQGTICIYVAIGQKDSTVRANVEKLRQAGAL
                     DYTIVVAASASEPSPLLYIAPYSGVTMGEEFMFNGKHVLIVYDDLTKQAAAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLNDDLGGGSITALPIIETQAGDISAYVPTNV
                     ISITDGQIFLQSDLFFSGVRPAINAGQSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     ESFAQFGSDLDEFTASKLERGKRTVEVLKQDQNKPLPVEHQVLIIYALTKGYLDDIPV
                     VDITRFEDELNHWAESNATELLNEIRETGGLPDAEKFDTAINEFKKSFSKSE"
     gene            complement(4759..5298)
                     /locus_tag="POB55_000404"
     CDS             complement(4759..5298)
                     /locus_tag="POB55_000404"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HDI6499400.1"
                     /translation="MVKVANKYAKALFDVSLDTNNLETINEELTVINEAVKDKIEQLK
                     MVDSNPTQTAEQRRELINGVFTDINPYIKNMMYVLADNRHISLIADVFKAFQSLYNGH
                     YNQDFATIESTYELSQEELDKIVKLVTQQTKLSKVIVDTKINPDLIGGFRVKVGTTVL
                     DGSVRNDLVQLQRKFRRVN"
     gene            complement(5298..5819)
                     /locus_tag="POB55_000405"
     CDS             complement(5298..5819)
                     /locus_tag="POB55_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HDI6499401.1"
                     /translation="MTETANLFVLGAAGGVEWGTVIVQVLTFIVLLALLKKFAWGPLK
                     DVMDKRERDINRDIDDAEQAKLNAQKLEEENKQKLKETQEEVQKILEDAKVQARQQQE
                     QIIHEANVRANGMIETAQSEINSQKERAIADINNQVSELSVLIASKVLRKEISEQDQK
                     ALVDKYLKEAGDK"
     gene            complement(6016..6228)
                     /gene="atpE"
                     /locus_tag="POB55_000406"
     CDS             complement(6016..6228)
                     /gene="atpE"
                     /locus_tag="POB55_000406"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HDI6499402.1"
                     /translation="MNLIAAAIAIGLSALGAGIGNGLIVSRTVEGVARQPEARGQLMG
                     IMFIGVGLVEALPIIGVVIAFMTFAG"
     gene            complement(6271..6999)
                     /gene="atpB"
                     /locus_tag="POB55_000407"
     CDS             complement(6271..6999)
                     /gene="atpB"
                     /locus_tag="POB55_000407"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HDI6499403.1"
                     /translation="MDHKSPLVSWNLFGFDIVFNLSSILMILVTAFLVFLLAIICTRN
                     LKKRPTGKQNFVEWIFDFVRGIIEGNMAWKKGGQFHFLAVTLILYIFIANMLGLPFSI
                     VTKDHTLWWKSPTADATVTLTLSTTIILLTHFYGIKMRGTKQYLKGYVQPFWPLAIIN
                     VFEEFTSTLTLGLRLYGNIFAGEILLTLLAGLFFNEPAWGWIISIPGLIVWQAFSIFV
                     GTIQAYIFIMLSMVYMSHKVADEH"
     gene            complement(7020..7472)
                     /locus_tag="POB55_000408"
     CDS             complement(7020..7472)
                     /locus_tag="POB55_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HDI6499404.1"
                     /translation="MNRIDTTNRLYYHLVCMSGTVFMIDISHTEAYKMSRFYNIFKQF
                     IQYYFYLIIILGGLYLYTHHAFILGLIIGVTGSAINTCIFECYLAKAKRPDTMHISTG
                     NMWRYLVAIIACMIWYLNKAHVSIIGIIIGLMISYVVVIIRPLLKVSK"
     gene            complement(7540..8667)
                     /gene="wecB"
                     /locus_tag="POB55_000409"
     CDS             complement(7540..8667)
                     /gene="wecB"
                     /locus_tag="POB55_000409"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HDI6499405.1"
                     /translation="MKKIMTIFGTRPEAIKMAPLVKALEQEKMLEPIVVVTAQHREML
                     DSVLSTFEIKPKYDLNIMKSGQTLSEITSKSITQLEQVIQLEKPDMVLVHGDTMTTFA
                     GGLAAFYNQVPIGHVEAGLRSYDKYSPFPEEVNRQLVGVLADLHFAPTKNAASHLLSE
                     GKYSESVVVTGNTAIDAMKYTVDDNYKSNIMDKYHDKKFILMTAHRRENIGKPMENIF
                     KAVRRLIDEYTDLALVYPMHKNPKVREVAQKILGSHDRIELIEPLDVVDFHNFAKKSY
                     FILTDSGGIQEEAPSFNKPVLVLRSVTERPEGVEAGTLKVIGTNKQNVYQAAKELIDD
                     ERLYHQMSEASNPYGDGFASERIVNHIKYYLNLITEKLSDF"
     gene            complement(8691..9320)
                     /gene="upp"
                     /locus_tag="POB55_000410"
     CDS             complement(8691..9320)
                     /gene="upp"
                     /locus_tag="POB55_000410"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017636774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HDI6499406.1"
                     /translation="MSKVHVFDHPLIQHKLSYIRDVNTGTKEFRELVDEVGMLMAYEV
                     TRDLELQDVDIETPVTKMTAKRLAGKKLAIVPILRAGLGMTDGILSLVPAARVGHIGL
                     YRDPETLKAVEYFAKLPQDITERQIIVVDPMLATGASAIEAITSLKKRGAKNIRFMCL
                     IAAPEGVEKMHEAHPDVDIYIAALDEKLNDKAYITPGLGDAGDRLFGTK"
     gene            complement(9348..10586)
                     /locus_tag="POB55_000411"
     CDS             complement(9348..10586)
                     /locus_tag="POB55_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDI6499407.1"
                     /translation="MSYITKQDKVIAEAIEREFQRQNSNIELIASENFVSEAVMEAQG
                     SVLTNKYAEGYPGRRYYGGCEFVDVTESIAIDRAKALFGAEHVNVQPHSGSQANMAVY
                     LVALEMGDTVLGMNLSHGGHLTHGAPVNFSGKFYNFVEYGVDKDTERINYDEVRKLAL
                     EHKPKLIVAGASAYSRTIDFKKFKEIADEVNAKLMVDMAHIAGLVAAGLHPNPVEYAD
                     FVTTTTHKTLRGPRGGMILCKEEYKKDIDKTIFPGIQGGPLEHVIAAKAVAFGEALEN
                     NFKTYQQQVVKNAKVLAEALINEGFRIVSGGTDNHLVAVDVKGSIGLTGKEAEETLDS
                     VGITCNKNTIPFDQEKPFVTSGIRLGTPAATTRGFDEKAFEEVAKIISLALKNSKDEE
                     KLQQAKERVAKLTAEYPLYQ"
     gene            complement(10613..11137)
                     /locus_tag="POB55_000412"
     CDS             complement(10613..11137)
                     /locus_tag="POB55_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HDI6499408.1"
                     /translation="MKDLTMLLDELKDMSFFNKGDICLIGCSTSEVIGEKIGTVGSME
                     VAETIFNALDVVSKETGVTFAFQGCEHINRAITIEKSQYNPLTMEEVSVVPDVHAGGS
                     LATYAFQHMKDPIVVEHITVPCGIDIGQTLIGMHIKHVCVPVRTSVKQVGQAIVTIAT
                     SRPKKIGGERAKYQ"
     gene            complement(11244..11663)
                     /locus_tag="POB55_000413"
     CDS             complement(11244..11663)
                     /locus_tag="POB55_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HDI6499409.1"
                     /translation="MKILFVCTGNTCRSPLAESIAKEVMPNHQFESRGIFAVNNQGVS
                     NYVEDLVEEHHLAETTLSQQFTEADLKADIILTMSYSHKELIEAHFGLQNHVFTLHEY
                     VKEAGEVIDPYGGTKEMYVHTYEELVSLILKLKDIIC"
     gene            complement(11660..12706)
                     /locus_tag="POB55_000414"
     CDS             complement(11660..12706)
                     /locus_tag="POB55_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HDI6499410.1"
                     /translation="MDTKIWDVREYNEDLQQYPKINEIKDIVLNGGLIGLPTETVYGL
                     AANATDEEAVAKIYEAKGRPSDNPLIVHIHSKGQLKDFTYTLDPRVEKLMQAFWPGPI
                     SFILPLKPGYLCRKVSGGLSSVAVRMPSHSVGRQLLQIINEPLAAPSANLSGRPSPTT
                     FNHVYQDLNGRIDGIVQAEQSEEGLESTVLDCTSFPYKIARPGSITAAMITEILPNSI
                     AHADYNDTEQPIAPGMKYKHYSPNTPLTIITDIESKIGNDGKDWSSIAFIVPSNKVAF
                     IPSEAQFIQLCQDDNDVKQASHNLYDVLHSLDENENISAAYIYGFELNDNTEAIMNRM
                     LKAASNHIIKGCEL"
     gene            complement(12790..13626)
                     /gene="prmC"
                     /locus_tag="POB55_000415"
     CDS             complement(12790..13626)
                     /gene="prmC"
                     /locus_tag="POB55_000415"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDI6499411.1"
                     /translation="MVNYKEKLDEAIHLTQQKGFEQTRAEWLMLDVFQWTRTDFVVHM
                     HDDMPKAMIMKFDLALQRMLLGEPIQYIVGFASFYGRTFDVNSNCLIPRPETEEVMLH
                     FLQQLEDDATIVDIGTGSGVLAITLKCEKPDLNVIATDISLEAMNMARNNAEKHQSQI
                     QFLTGDALKPLINEDIKLNGLISNPPYIDEKDMVTMSPTVTRFEPHQALFADNHGYAI
                     YESIIEDLPHVMEKGSPVVFEIGYNQGEALKSIILNKFPDKKIDIIKDINGHDRIVSF
                     KW"
     gene            complement(13613..14689)
                     /gene="prfA"
                     /locus_tag="POB55_000416"
     CDS             complement(13613..14689)
                     /gene="prfA"
                     /locus_tag="POB55_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015445905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HDI6499412.1"
                     /translation="MFDQLDIVEERYEQLNELLSDPDVVNDSDKLRKYSKEQADLQKT
                     VDVYRNYKAKKEELADIEEMLSETDDKEEVEMLKEESNGIKAELPNLEEELKILLIPK
                     DPNDDKDVIVEIRAAAGGDEAAIFAGDLMRMYSKYAESQGFKTEIVEASESDHGGYKE
                     ISFSVSGNGAYSKLKFENGAHRVQRVPETESGGRIHTSTATVAVLPEVEDVEIEIRNE
                     DLKIDTYRSSGAGGQHVNTTDSAVRITHLPTGVIATSSEKSQIQNREKAMKVLKARLY
                     DMKVQEEQQKYASQRKSAVGTGDRSERIRTYNYPQSRVTDHRIGLTLQKLGQIMEGHL
                     EEIIDALTLSEQTDKLKELNNGEL"
     gene            complement(14690..15289)
                     /locus_tag="POB55_000417"
     CDS             complement(14690..15289)
                     /locus_tag="POB55_000417"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HDI6499413.1"
                     /translation="MYETYHSGWIECITGSMFSGKSEELIRRLRRGIYAKQKVVVFKP
                     AIDDRYHKEKVVSHNGNAIEAINISKASEIMTHDLTNVDVIGIDEVQFFDDEIVSIVE
                     KLSADGHRVIVAGLDMDFRGEPFEPMPKLMAVSEQVTKLQAVCAVCGSSSSRTQRLIN
                     GKPAKIDDPIILVGANESYEPRCRAHHIVAPSDNNKEEL"
     gene            complement(15636..15890)
                     /locus_tag="POB55_000418"
     CDS             complement(15636..15890)
                     /locus_tag="POB55_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HDI6499414.1"
                     /translation="MKQGIHPEYHQVIFLDTTTNFKFLSGSTKTSSEMMEWEDGKEYP
                     VIRLDISSDSHPFYTGRQKFAAADGRVERFNKKFGLKSNN"
     gene            complement(16008..17324)
                     /gene="rho"
                     /locus_tag="POB55_000419"
     CDS             complement(16008..17324)
                     /gene="rho"
                     /locus_tag="POB55_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HDI6499415.1"
                     /translation="MPERERTSPQYESFHELYKNYTTKELTQKAKTLKLTNYSKLNKK
                     ELVLAIMEAQMEKDGNYYMEGILDDIQPDGYGFLRTVNYSKGEKDIYISASQIRRFEI
                     KRGDKVTGKVRKPKDNEKYYGLLQVDFVNDHNAEEVKKRPHFQALTPLYPDERIKLET
                     ETQNYSTRIMDLVTPIGLGQRGLIVAPPKAGKTSLLKEIANAISTNKPDAKLFILLVG
                     ERPEEVTDLERSVEAAEVVHSTFDEPPEHHVKVAELLLERAKRLVEIGEDVIILMDSI
                     TRLARAYNLVIPPSGRTLSGGLDPASLHKPKAFFGAARNIEAGGSLTILATALVDTGS
                     RMDDMIYEEFKGTGNMELHLDRKLSERRIFPAIEIGRSSTRKEELLISKSELDTLWQL
                     RNLFTDSTDFTERFIRKLKRSKNNEDFFKQLQKSAEESTKTGRPII"
     gene            complement(17571..18998)
                     /locus_tag="POB55_000420"
     CDS             complement(17571..18998)
                     /locus_tag="POB55_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HDI6499416.1"
                     /translation="MRDYTKQYINGEWVESNSNETIEVINPATEEVIGKVAKGNKADV
                     DKAVEAADNVYLEFRHTSVKERQALLDKIVKEYENRKDDIVQAITDELGAPLSLSERV
                     HYQMGLNHFVAARDALDNYEFEERRGDDLVVKEAIGVSGLITPWNFPTNQTSLKLAAA
                     FAAGSPVVLKPSEETPFAAVILAEIFDKVGVPKGVFNLVNGDGAGVGNPLSEHPKVRM
                     MSFTGSGPTGSKIMEKAAKDFKKVSLELGGKSPYIVLDDVDIKEAAKATTGKVVNNTG
                     QVCTAGTRVLVPKKIKDAFLAELKEQFSQVRVGNPREDGTQVGPIISKKQFDQVQNYI
                     NKGIEEGAELFYGGPGKPEGLEKGYFARPTIFINVDNQMTIAQEEIFGPVMSVITYND
                     LDEAIQIANDTKYGLAGYVIGKDKETLHKVARSIEAGTVEINEAGRKPDLPFGGYKQS
                     GLGREWGDYGIEEFLEVKSIAGYFK"
     gene            complement(19236..19571)
                     /locus_tag="POB55_000421"
     CDS             complement(19236..19571)
                     /locus_tag="POB55_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI6499417.1"
                     /translation="MEVCPYLEETFKILGRSWNGLIINYLSRCNDCSAHFSDMKRDLK
                     TITPRALSLKLSELAQWELVEKQIISTSPVQIIYVLTEKGKALAEALHPIEAWAQSYV
                     DLTDQRTAK"
     gene            complement(19660..20919)
                     /locus_tag="POB55_000422"
     CDS             complement(19660..20919)
                     /locus_tag="POB55_000422"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDI6499418.1"
                     /translation="MAQEVIKIRGGRTLNGEVNISGAKNSAVAIIPATLLAQGHVKLE
                     GLPQISDVKTLVSLLEDLNIKASLNGMELEVDTTEIQNAALPNNKVESLRASYYMMGA
                     MLGRFKKCVIGLPGGCPLGPRPIDQHIKGFKALGAEIDESSTTSMKIEAKELKGAHIF
                     LDMVSVGATINIMLAAVYATGQTVIENAAKEPEVVDVANFLTSMGANIKGAGTSTIKI
                     NGVKELHGSEYQVIPDRIEAGTYMCIAAACGENVILNNIVPKHVETLTAKFSELGVNV
                     DVRDERIRINNNAPYQFVDIKTLVYPGFATDLQQPITPLLFMANGPSFVTDTIYPERF
                     KHVEELKRMGANIEVDEGTATIKPSTLHGAEVYASDLRAGACLIIAGLIAEGVTTIYN
                     VKHIYRGYTDIVEHLKALGADIWTETV"
     gene            complement(21385..22245)
                     /locus_tag="POB55_000423"
     CDS             complement(21385..22245)
                     /locus_tag="POB55_000423"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002468309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HDI6499419.1"
                     /translation="MPLVSMKEMLIDAKENGYAVGQYNINNLEFTQAILEASQEENAP
                     VILGVSEGAARYMSGFYTIVKMVEGLMHDLNITIPVAIHLDHGSSFEKCKEAIDAGFT
                     SVMIDASHSPFEENVATTKKVVEYAHEKGVSVEAELGTVGGQEDDVVADGIIYADPKE
                     CQELVEKTGIDALAPALGSVHGPYKGEPKLGFKEMEEIGLSTGLPLVLHGGTGIPTKD
                     IQKAIPFGTAKINVNTENQIASAKAVRDVLNNDKEVYDPRKYLGPAREAIKETVKGKI
                     KEFGTSNRAK"
     gene            22463..22984
                     /locus_tag="POB55_000424"
     CDS             22463..22984
                     /locus_tag="POB55_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2529 domain-containing protein"
                     /protein_id="HDI6499420.1"
                     /translation="MSKILNTQLIGIFNRLEKQSLEIQMAAQCLIQAIGGEGYVYVKG
                     YDDLQFFESFILHSDERLKSSRKLDAIKDFKEIDSTDRVLLFAPFYNDQVALDIQKLI
                     DLDIDVVLISNKPKTDDFPDHLVHFIDLSTPRPIVYTEDYDKIVQPHAMALNYVYYDI
                     YTQMIEMTRDLEL"
     gene            complement(23093..24703)
                     /locus_tag="POB55_000425"
     CDS             complement(23093..24703)
                     /locus_tag="POB55_000425"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HDI6499421.1"
                     /translation="MTKFIFVTGGVVSSLGKGITASSLGRLLKDRGLNVTIQKFDPYL
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKFSNVTAGKVYSHVLKKE
                     RRGDYLGGTVQVIPHITNEIKERLLLAGESTNADVVITEIGGTTGDIESLPFIEAIRQ
                     IRSDLGRENVMYVHCTLLPYIKAAGEMKTKPTQHSVKELRGLGIQPDLIVVRTEYEMT
                     QDLKDKIALFCDINKESVIECRDADSLYEIPLQLSQQNMDDIVIKRLQLNAKYETQLD
                     EWKQLLDIVNNLDGKITIGLVGKYVSLQDAYLSVVESLKHAGYPFAKDIDIRWIDSSE
                     VTDENAAEYLADVDGILVPGGFGFRASEGKISAIKYARENNVPFFGICLGMQLATVEF
                     SRNVLGLEGAHSAELDPATPYPIIDLLPEQKDIEDLGGTLRLGLYPCSIKEGTLAQDV
                     YGKAEIEERHRHRYEFNNDYREQLEANGMVISGTSPDGRLVEMVEIPTNDFFIACQFH
                     PEFLSRPNRPHPIFKSFIEASLKYQQNK"
     gene            complement(25039..25569)
                     /locus_tag="POB55_000426"
     CDS             complement(25039..25569)
                     /locus_tag="POB55_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HDI6499422.1"
                     /translation="MKIQDYTKQMVDEKSFIDMAYTLLNDKGETMNLYDIIDEFRALG
                     DYEYEEIENRVVQFYTDLNTDGRFLNVGENLWGLRDWYSVDDIEEKIAPTIQKFDILD
                     ADDEEDQNLKLLGEDEMDDDDDIPAQTDDQEELNDPEDEQVEEEINHSDIVIEEDEDE
                     LDEDEEVFEDEEDFND"
     gene            complement(25681..26541)
                     /locus_tag="POB55_000427"
     CDS             complement(25681..26541)
                     /locus_tag="POB55_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6499423.1"
                     /translation="MAKELCFEGITLKAFDEQYRSAINDFDLNERQQIYSSLPKEVID
                     DAINDADRIANVAINDKNEVVGFFVLHRYYQHEGYDTPENVVYIRSLSINEKYQGFGY
                     GTKIMMSLPQYVQGVFPDFNHLYLVVDAENDNAWNLYERAGFMHTATKEEGPIGKERL
                     YYLDLDSKHVSSLKLEEESRSEVTNVHIINLMIDGQKVGFIALEQIGERMNIAAIEVD
                     KSYRFNGIGSSALRQLPTYLRKNYDNLNVITMILFGENNDFKPLCLNSNFVEIEQTDD
                     YVVFEKYLNY"
     gene            26965..27768
                     /gene="coaW"
                     /locus_tag="POB55_000428"
     CDS             26965..27768
                     /gene="coaW"
                     /locus_tag="POB55_000428"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II pantothenate kinase"
                     /protein_id="HDI6499424.1"
                     /translation="MKVGIDAGGTLIKIVQEQDNQRTFKTELTKNIDQVVEWLNQQQI
                     EKLCLTGGNAGVIAENINIPAQIFVEFDAASQGLGILLKEQGHDLADYIFANVGTGTS
                     LHYFDGQSQRRVGGIGTGGGMIQGLGYLLSQITDYKQLTDMAQHGDRNTIDLKVRHIY
                     KDTEPPIPGDLTAANFGHVLHHLDADFTPSNKLAAVIGVVGEVVTTMAITVAREFKTE
                     NIVYIGSSFHNNALLRKVVEDYTVLRGCKPYYVENGAFSGAIGALYLEK"
     gene            complement(27898..28569)
                     /locus_tag="POB55_000429"
     CDS             complement(27898..28569)
                     /locus_tag="POB55_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2750 domain-containing protein"
                     /protein_id="HDI6499425.1"
                     /translation="MSYKAYPFFRDILINECIYFASKNKKLVRLNYKSEAYVGVWTEE
                     SVAVSFLTSRDIPFDKVVKMDVDRFATYELDELFDEQDHIIMNQTMEEEGHLLNVVAV
                     TQEVMTELDKIRIKEFVQDVAKYDEVYGLTKKGSKQFILISENDSDEKKPHIMPVWSI
                     KNRALKVRDEDFEECDLITIEGSVFGEWLDELRDDHKAVAIDLKTGVVGTIVSAQKLS
                     NELTF"
     gene            complement(28968..30161)
                     /locus_tag="POB55_000430"
     CDS             complement(28968..30161)
                     /locus_tag="POB55_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HDI6499426.1"
                     /translation="MTNNGNEPNLTLSDLYDKDVVYTSRPSYISNPWLKPDEHQSNFL
                     TGRELLIANQLPVIVHEASATDKLHQLFQVIGKEVPNSIYTFNNQQSYENLIKQLAHK
                     ENKKIYFQYIHDETILNQQYYALDKTLFVALNNKARIPEWTNGKFLPKRKVVKIEQFE
                     NEIKNWEFPLVIKPGDDLPTAGGYGVMICYHDADLQKAITRIKEATAETNSLIIEQKI
                     EEKANYCVQFAYSESLGIQYLGAATQLTDKYGFYNGNENTTNVPEHVIEAGRQIMENG
                     VNQGFFGVAGFDLLVDEDDNVYAIDLNFRQNGSTSMLLLANELNSGYQKFYSYHSKGD
                     NTHFFNTILKYVKEGSLYPLSYYDGDWYGEDKVKSRFGCIWHGDSKETVLENERAFLA
                     ELEHY"
     gene            complement(30161..31345)
                     /locus_tag="POB55_000431"
     CDS             complement(30161..31345)
                     /locus_tag="POB55_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDI6499427.1"
                     /translation="MSEKQQILDYIETNKYSYIEISHRIHERPELGNEEIFASRTLID
                     RLKEHDFEIETEIAGHATGFIATYDSGLDGPAIGFLAEYDALPGLGHACGHNIIGTAS
                     VLGAIGLKQVIDQIGGKVVVLGCPAEEGGENGSAKASYVKAGVIDQIDIALMIHPGNE
                     TYKTIDTLAVDVLDVKFYGKSAHASENADEALNALDAMISYFNGVAQLRQHIKKDQRV
                     HGVILDGGKAANIIPDYTHARFYTRAMTRKELDILTEKVNQIARGAAIQTGCDYEFGR
                     IQNGVNEFIKTPKLDDLFAKYAEEVGEAVIDDDFGYGSTDTGNVSHVVPTIHPHIKIG
                     SRNLVGHTHRFREAAASVHGDEALIKGAKIMALMGLELITNQDVYQDIIEEHAHLKGN
                     GK"
BASE COUNT         9509 a         6242 c         4522 g        11189 t
ORIGIN      
        1 taagctaact tactatataa ttctttttac ataaatatca taactataaa gtatcttggt
       61 atgaaaaaat gtagctactt tcaaaaaatg aaatgtcgac tgtagatagt ggtgaatgaa
      121 attttgattt tagcaatcta ttagtgattg taaaaaagta aaactattct tgggagtcgg
      181 acataaatca atgtctagac tctatagtgt tgtatttggc agtagttgac ggggccccaa
      241 cataaagaaa tactttttct ttagaaatta gtatttctta tacatgagtt ttactcatgt
      301 attcctattt ttaaatacac attagctgtg tttaatgata aagaaccact acatcataaa
      361 tctttaatag ttctttatca attccttccc ccctctcatg ttttgtatct ttaaatcaat
      421 ataattcaaa aaagggtcga agatatgaaa tcacatcttc gaccctttat ttactatttt
      481 aattcagcca cacgcaaacg gttatttgct ctctctaaag ctctttcggc tctatgaata
      541 tcagtattgt cgtcatcatt ttccaagtga gactctgctc ttgctttggc taatttagct
      601 ctttcaacat caatttctct tgcagtttct gcagtctgaa caatgattga aactttatct
      661 tttctaactt caacaaagcc atcgcttaca gcaatatatt cagttccatc gtgaaatttc
      721 acttttacaa agcctgtttt taaagcagct acagttggaa tatgtccact catgacacct
      781 atctcaccag ctgttgtttg cataacaacg agttcaacat tatcacgatt gtaaacagaa
      841 ccattaggag tgacaatatc taggtttaat gtattcatta tccattcctc ctaattgtta
      901 tacttcaaca cccatatctt tagcttttgc aataacatca tccatgctac caactaaacg
      961 gaaagcatct tctggaatat ggtcatattt accatctaag atatctttaa agtttgcaac
     1021 tgttgtctta acaggtacat aagaaccttt ttgaccagta aattgttccg ctacgtggaa
     1081 gttttgagat aagaagaatt gaattctacg tgcgcgttca actgtttgtt tatcttcatc
     1141 agataattcg tccataccta agatagcaat gatatcttgt aattcacggt atttttgaag
     1201 tgttgattgt acatcacgag ctacttcata atgttcttga cctacaattg atggttccaa
     1261 tgctcttgat gtagacgcta atggatccac ggctggataa atacccattt cagttaattt
     1321 acgttctaag tttgtagttg catctaaatg ggcaaacgct gtcgcaggcg ctgggtcagt
     1381 atagtcatcg gcaggtacga ataccgcttg aatagaagta actgatcctt ttgttgtaga
     1441 cgtaatacgt tcttgtaatt gtcccatttc agtagcaagt gttggttggt aacctactgc
     1501 agaaggcata cgacctaata atgcagatac ctcagaacca gcttgtgtaa atctgaaaat
     1561 gttatcgatg aataataata cgtcttgacc ttgttcgtca cggaaatatt cagccattgt
     1621 taaaccagat aatgcaacac gcatacgtgc accaggtggc tcattcattt gcccgaatac
     1681 catggctgtt ttcttaatta caccactgtc actcatttcg aagtataaat cgttaccttc
     1741 acgagtacgt tcacctacac cggcgaatac agaaatacca ccgtgctctt gagcgatgtt
     1801 gttaattaat tcttggatta atactgtttt acctacaccg gcaccaccga acaatccgat
     1861 tttaccacct ttaatataag gtgctagtaa atctactact ttaatacctg tttctaaaat
     1921 ttgaacttct gttgaaagtt catcgaatgc tggtgcttga cgatggatag gatcgcggcg
     1981 aacagaatca ctaatttctt ctttaaggtc aattgtttca cctagtacat taaatacacg
     2041 acctaatgtt tcgtcaccaa caggtacact aatttctttg cctgtatctt ttacatccat
     2101 gcctctttgg acaccatcag ttgaatccat cgcaattgta cgaacaacgt cgtcacctaa
     2161 ttgcagcgca acttctaatg ttagttgtat tgtaccttct tctttaggca catcaataac
     2221 caaggcgtta ttaattttag gaacttcgtt atgttcaaat cgaacatcaa ttacaggacc
     2281 cataacttga gttacacggc caattcccat gctattttcc tcctttaaat attattcaag
     2341 cgctgcggaa ccaccaacaa tttcagtaat ttgttgcgta atttctgctt gtctcgctct
     2401 gttatattct aatgataagt catcaataag ttcagttgca ttatcagtgg catttttcat
     2461 cgcagtcata cgtgttgcat gctcacttgc ttttgcgtct aatattgttc cgtaaatcaa
     2521 actctcaaca tattgaggca agattacact taagatagat tctttatctg gctcaaattc
     2581 ataagaagac aaatgaccat gccccttact agaatcctct tgagataatg gtaatacttg
     2641 tctagatgta ggcttgtttt caagaacgct gacataatga ctatagtata tatttaattc
     2701 atcaatttct tcttcactgt ataagtctat agcatggtta gctagtgctt gaacagattt
     2761 gaaagaaggt tgatcaggta cgtctacttg agaatactca atgtcataac ctctattttt
     2821 taggaaatca acaccttgtt gccctaaaac tacaatactg tattcgctac tatcttgatg
     2881 tttcgcttca atatcagtaa tcaatttttt aagcacgttt gcactatatg cacctgctaa
     2941 acctttatca ctcgtgataa ctaaatatcc acttctagta attttacgag gtcttagcat
     3001 tggatggttt gtattgctac ttgcccctgc aactgcagta attgcatctt gcattttatc
     3061 catatatggt gtgaattgtt ttgtattttt ttcagctcta cgaagttttg aacttgatac
     3121 catgttcatc gctttcgtaa tctgcttcat ttttttggtt gattttattc gagtatctat
     3181 ttctttaaga gaagccacta tctcaccacc ttatactaaa cttgtttatt attcagattt
     3241 gctaaagctt tttttgaatt cgttaattgc tgtgtcaaac ttctcagcat ctggtaagcc
     3301 accagtttct ctgatttcat ttaacagttc agtagcattt gattctgccc agtggtttaa
     3361 ctcatcttca aaacgtgtga tatctacaac tggaatatca tctaaatatc cttttgttaa
     3421 tgcataaata atcaacactt ggtgttcgac tggtaatggt ttgttttgat cttgttttaa
     3481 gacttcaaca gtacgtttac cacgttctaa tttacttgca gtaaattcat caaggtctga
     3541 accgaattgt gcaaatgatt caagttctct gtatgacgct aagtcaagac gtaacgtacc
     3601 agcaactttc ttcattgctt taatttgtgc agatccacca acacgagata cagattgtcc
     3661 agcattaatc gctggtctta cacctgagaa gaataaatca gattgtaaga agatttgtcc
     3721 atctgtaatt gaaataacgt ttgttggtac ataagctgaa atatcaccag cttgtgtttc
     3781 gataattggt aatgcagtaa ttgaaccgcc acctaagtca tcgtttaatt ttgctgctct
     3841 ttctaataat ctactatgta agtagaatac gtcacctggg tatgcttcac gacctggagg
     3901 tctacgtaat aataatgata actcacgata agctgctgct tgtttagtta aatcatcata
     3961 aacgattaaa acatgtttac cgttaaacat gaattcttca cccattgtta cacctgaata
     4021 tggtgcaata tataataatg gagaaggttc agaagctgat gctgctacaa caatagtgta
     4081 gtctaaagcg cctgcttgtc ttaacttttc aacatttgct cttactgttg aatctttttg
     4141 accaatagca acatagatac aaatcgtacc ttgatctttt tggttcaaaa ttgtgtcaat
     4201 tgcaattgtt gttttacctg tttgacggtc accgatgatt aactcacgtt gacctctacc
     4261 aattggtact aaagcatcaa ttgctttgat acctgtttgt aatggctcat ctactgattt
     4321 acgatccatt acaccagtag cttttttctc tactggacgt gttttagttg tgttaatcgg
     4381 tccttgtcca tcaataggtt gtcctaatgg attaacaact cttccgatta gttcttcacc
     4441 tactggtact tccatgatac gaccagtacg tttaacttcg tcaccttcag taatacctgt
     4501 gtatggtcct aaaataacca cacccacgtt tgactcttca aggttttggg ctaaaccaag
     4561 tacgccgtta tggaattcta ctagctcacc agccataacg tcatttaatc cgtgaattaa
     4621 tgcaatacca tcaccaattt gtaatacagt acctacatca gttacggaca tttctgactc
     4681 ataattttca atttgtgagc gaagtaatgc actgatttct tcagctttta tggccatcta
     4741 tgtcactcct ctttataatt aattaactct tctaaatttt ctttgtaatt ggacaagatc
     4801 atttctaaca ctaccatcta atacagttgt gccgacttta actctaaatc caccaattaa
     4861 atctggatta atttttgtat ctacaataac tttagataac ttcgtttgtt gagttactag
     4921 tttgacaatc ttatctaact cttcttgact caattcatat gttgactcaa ttgttgcaaa
     4981 atcttgattg tagtgtccgt tatataagct ttggaacgcc ttgaatacgt cagctattaa
     5041 tgagatatgt ctattatctg ctaacacgta catcatattt ttaatgtatg gattaatgtc
     5101 agtaaataca ccattaatca actcacgacg ttgttctgct gtttgagttg gattactgtc
     5161 aaccattttc aattgctcaa ttttatcttt tactgcttca tttataactg ttaattcttc
     5221 attaatagtc tctaaattat ttgtatctaa tgacacgtca aataatgctt tagcatactt
     5281 gttagctact tttaccatta tttatcgcct gcctctttta gatacttgtc aaccaatgct
     5341 ttttggtctt gttcagaaat ttcttttcta agaactttag aagcaattaa cactgatagt
     5401 tcagatactt gattattaat atctgcaatg gcacgttctt tttggctatt gatttcactt
     5461 tgtgctgttt caatcatacc gtttgcacgt acgtttgctt catgaataat ttgttcttgc
     5521 tgttgacgtg cttgaacctt agcatcttct aaaatctttt gaacttcttc ttgtgtttct
     5581 ttaagttttt gtttattttc ttcttcaagt ttctgtgcat ttaacttagc ttgttctgcg
     5641 tcatcgatat ctctgttaat atctctttca cgtttatcca ttacatcttt caatggaccc
     5701 catgcgaact ttttaagtaa cgcaagtaac acgatgaaag ttaggacctg tacaatcaca
     5761 gtaccccact caacgcctcc agctgcacca agaacgaata agttagctgt ttcagtcacg
     5821 ggttcatgac actcctttca ttaaaataat cgctagtgtt atatagaaaa aacgacgagg
     5881 ttaggaccta ctgaatttat gcgataggca tggtgcattc atatctcaac tatttaataa
     5941 agttcaaaga tattactata tatgcatcat gaattatgtc ctatgcgctt tgtcccgacc
     6001 tcttttatct gttaattatc cagcaaatgt catgaatgca attactacac cgatgatagg
     6061 taatgcctca actaaaccta caccaatgaa catgataccc attaattgac cacgtgcttc
     6121 tggttgacgt gctacacctt caactgttct tgaaacgatt aaaccgttac cgatacctgc
     6181 tcctaatgct gataaaccaa ttgcgattgc tgctgcgatt aaattcataa tttaatttcc
     6241 tcctgtgatt gtatataatt attgaaattt ttagtgttca tctgccactt tatgtgacat
     6301 ataaaccatc gaaagcataa taaagatata tgcttggatt gttcctacaa atattgaaaa
     6361 tgcttgccaa acaattaatc ctgggatact aataatccaa ccccatgctg gttcgttaaa
     6421 gaataagcca gcaagtaatg ttaatagtat ctcacctgca aatatgttac cgtacaaacg
     6481 cagaccaagc gttaatgttg aagtgaactc ttcgaaaaca ttaataattg ccaatggcca
     6541 aaacggctgt acataacctt taagatattg tttcgtacca cgcattttaa ttccataaaa
     6601 gtgagttaac agtattatcg ttgtagacaa cgttaaagtc actgttgcat cagctgtcgg
     6661 tgatttccac cacaatgtgt gatctttcgt tactatagaa aacggaagac ctaacatatt
     6721 agctataaaa atgtacagaa tcagcgttac tgctaagaag tggaattgac cacctttttt
     6781 ccaagccatg ttaccttcaa tgattcccct cacgaaatca aaaatccatt caacgaaatt
     6841 ttgtttgcca gttggtcttt ttttcaaatt acgcgtacaa atgatagcaa gtagaaaaac
     6901 aagaaacgcc gtaacaagta tcatcaatat acttgataaa ttgaaaacga tatcaaaacc
     6961 gaataaattc caactcacga gcggggattt gtgatccata atctcacctc tttcttaatt
     7021 tatttgctca cctttagtaa aggacgtatg ataactacaa catatgaaat cattaaacca
     7081 ataattatac cgatgatact tacatgcgct ttattaaggt accaaatcat acaggcaata
     7141 attgcaacta agtatcgcca catatttcca gttgaaatat gcatagtgtc tggtctttta
     7201 gctttagcta aatagcattc aaaaatacat gtgttgattg cagaaccagt aacaccaatg
     7261 attaatccta agataaatgc atggtgtgta taaaggtata atcctcccaa tattattatt
     7321 agataaaaat aatattgaat gaactgttta aaaatgttgt agaaacgact cattttatat
     7381 gcctccgtgt gactgatatc aatcatgaaa accgttccac tcatacaaac taaatgataa
     7441 tataggcgat ttgtggtgtc aattctattc aaattgttgt aaaaaaataa gcatagttag
     7501 acacaattta gacacaatta gacaaaaagt ctattttcct cagaaatcgc ttagtttttc
     7561 agtgattaaa ttcaaataat acttaatatg attaactatt ctttctgaag caaatccatc
     7621 accgtaagga ttagatgctt cagacatttg atgatataat ctctcatcat cgattaattc
     7681 ttttgcagct tgatacacat tttgcttatt tgttccaatg acttttaatg tgccagcttc
     7741 aacaccttca ggacgttctg taacgcttcg caaaactaaa actggcttat taaatgatgg
     7801 cgcttcttcc tgaattccac ctgaatctgt caaaataaaa taagattttt tagcaaaatt
     7861 atggaaatct actacgtcca aaggttcaat caattcaatt ctgtcatgac tacctaaaat
     7921 cttttgagcc acctctcgaa ctttcgggtt tttatgcatt ggatatacca gtgctaaatc
     7981 agtatactca tctattaagc gtctaaccgc tttaaatata ttttccatgg gtttcccgat
     8041 attttctcgt cggtgtgctg tcataagaat gaattttttg tcatggtatt tatccatgat
     8101 gttagattta taattgtcat caactgtata tttcatagca tcaatcgcag tattaccagt
     8161 gacaacaaca ctttctgaat atttcccttc acttaacaaa tgcgatgcag catttttagt
     8221 aggtgcaaaa tgtaagtcag ctaatacacc aactaattgt ctattcacct cttccggaaa
     8281 aggtgaatat ttatcataac ttctaagccc tgcttcaacg tgtccaatcg gcacttggtt
     8341 ataaaatgcc gctaaaccac ctgcaaatgt cgtcatcgta tcaccatgta caagtaccat
     8401 gtctggtttt tctaattgaa tcacttgttc taattgagtg attgatttag aagttatctc
     8461 agaaagtgtc tgtcctgatt tcataatatt caaatcgtat tttggtttga tttcaaaggt
     8521 acttaatact gaatcaagca tttctctatg ctgtgctgta acaacaacaa ttggctcgag
     8581 cattttttct tgttccaaag ctttaactaa aggagccatc tttatggctt cgggtcttgt
     8641 tccaaatatg gtcataatct ttttcatcaa actacttatc tccgattctt ctatttagta
     8701 ccaaacaatc tatctccagc gtcgcctaac cctggtgtga tatatgcttt atcatttagc
     8761 ttttcatcaa gtgcagcaat ataaatatct acatctggat gtgcttcatg catcttttct
     8821 acgccttctg gtgctgcaat taaacacatg aagcgaatat ttttagcgcc acgtttcttc
     8881 aatgaagtaa tagcttcaat tgctgatgcg cctgttgcta acataggatc aacaacaatg
     8941 atttgtcttt cagtaatatc ttgaggtaac ttagcaaaat actctacagc ctttaatgtt
     9001 tcgggatctc gatacaaacc gatatgtcca actctggctg caggcactaa acttaaaata
     9061 ccatcagtca tacctaaacc agctcttaaa attggaacga tagctaattt tttaccagct
     9121 aatcgtttag ccgtcatttt agttacaggc gtttcaatat caacatcctg aagctctaag
     9181 tctctagtta cttcatatgc catcaacata ccaacttcgt ctacaagttc tctaaattct
     9241 ttagtacctg tatttacatc tctaatatag cttagtttgt gttgaattaa tggatgatcg
     9301 aaaacgtgta ctttactcat aaaaattact cctatctttg tgtatgttta ttgatataga
     9361 ggatattcag ctgttaattt cgcaacgcgt tctttagctt gttgtaattt ttcttcatct
     9421 ttactatttt tcaatgctaa actgatgatt tttgcaactt cctcaaaagc tttttcatca
     9481 aatccacgcg ttgttgcagc aggtgtacct aaacgtatac cactcgttac aaaaggtttt
     9541 tcttgatcga acggaatggt atttttgtta catgtgatac caactgaatc taaagtctct
     9601 tcagcttctt taccagtaag tcctatagac ccttttacat caacagctac taagtgatta
     9661 tctgtaccgc cagaaacaat tctaaatcct tcattaatta atgcttctgc aagaactttt
     9721 gcgtttttaa ccacttgttg ttgatacgtt ttgaaattat tttctaacgc ttctccaaaa
     9781 gcaactgctt ttgctgcaat aacatgctca agaggtccac cttgaatacc agggaaaatt
     9841 gttttatcta tgtctttttt atattcttcc ttacataaaa tcataccacc acgtggtccg
     9901 cgtaatgttt tgtgtgttgt agttgttaca aaatcagcat attctactgg atttggatgt
     9961 aaacctgccg ctactaatcc tgcaatatgt gccatgtcta ccattaactt agcgtttact
    10021 tcatctgcga tttctttaaa ctttttgaag tcaattgttc ttgaatatgc tgatgctcct
    10081 gccacaataa gcttaggctt atgctctaac gctaatttac gaacttcatc ataattgatt
    10141 cgttctgtgt ctttatctac tccatattca acgaaattgt agaatttacc actaaaatta
    10201 acaggcgctc catgtgtcaa gtgaccacca tgactcaaat tcatacctaa aactgtgtcg
    10261 cccatttcta atgcaactaa gtaaacagcc atgttcgctt gtgaacctga atgtggttga
    10321 acattgacat gttcagctcc aaacaatgct ttagcacgat caattgcgat gctttcagta
    10381 acatctacaa actcacagcc accataatat cggcgtcctg gatagccttc agcatactta
    10441 ttagtcaaca ctgaaccttg tgcttccata accgcttccg atacaaaatt ttccgatgcg
    10501 attaactcta tgttgctatt ttgtctctga aattctctct cgattgcttc tgcgataact
    10561 ttatcttgct tggtgatata agacataaaa tctccccttc tttcaaaaaa acttattggt
    10621 atttagcacg ttcgccacca atctttttcg gcctagatgt ggcaatagtt acaattgcct
    10681 gtcctacttg ctttactgag gtccttacag gtacacatac atgtttaata tgcatgccta
    10741 ttaacgtttg accaatatca attccacaag gaacagtaat atgttcgacc acgatcggat
    10801 ccttcatatg ctgaaaagcg tatgttgcca aactccctcc agcatgtaca tctggaacga
    10861 cggaaacttc ttccattgtt aatggattat actgagattt ttctattgtt atcgctctgt
    10921 tgatatgttc acatccttga aaagcaaaag taacgcctgt ctctttactc acaacatcta
    10981 atgcattaaa aatagtttct gcaacttcca tcgaaccgac agtccctatt ttttcgccaa
    11041 tgacttccga tgttgaacat ccaattaaac atatatctcc tttattaaaa aaggacatat
    11101 cttttaattc gtctaataac attgtcaaat ctttcataaa agcccaccct tcctaaaaat
    11161 aaaaaaggaa tatagcaaag tgctacactc ctctattata acttatttaa ctgttaacat
    11221 atactaatta tacagaattc ctactagcaa ataatatctt ttaattttaa aattaaactt
    11281 acaagttctt cataggtatg tacatacatt tcttttgttc caccgtatgg atctataact
    11341 tctcctgctt cttttacata ttcatgcaat gtgaaaacat gattttgcaa accaaagtgt
    11401 gcctctatta attctttgtg cgaatacgac atcgtcaaaa taatatctgc tttcaaatct
    11461 gcttcagtaa attgttgcga taaggtcgtt tcagctaaat gatgttcttc aactaagtct
    11521 tcaacataat tcgaaacacc ttgattgttc acagcgaata tacctcttga ttcaaattga
    11581 tgatttggca taacctcttt tgcaatactt tccgctaatg ggctacgaca tgtgttacct
    11641 gtacaaacga ataaaatctt catagttcac atcctttaat aatgtgatta cttgcagctt
    11701 ttaacatgcg attcataatt gcttctgtat tatcattcag ctcaaagccg tatatatacg
    11761 ccgctgaaat attttcattt tcatcaagtg aatgtaacac atcataaaga ttatgacttg
    11821 cttgtttaac atcattgtca tcctgacata attgaatgaa ttgcgcttca cttggtataa
    11881 acgccacctt attactcggc acaataaaag ctatagaaga ccaatcttta ccgtcatttc
    11941 caattttgct ctcaatatct gtaataattg taagtggtgt attgggtgag taatgcttat
    12001 acttcatacc tggtgcaatt ggctgttcag tatcattata atcagcatgg gcgatactat
    12061 tcggaagtat ttctgtaatc attgctgctg ttatagaacc aggtcttgca attttataag
    12121 gaaaagatgt gcaatctaaa accgtacttt ctaatccttc ttcactttgt tcagcttgaa
    12181 caataccatc gatacggcca ttcaaatctt gatatacatg attgaaagtt gttggtgaag
    12241 gtctaccact taaattagca cttggagcag ctagaggttc atttatgatt tgtaataatt
    12301 gtctacctac agaatggctt ggcattctaa cagcaactga tgataaacct ccagaaactt
    12361 ttcgacatag atagcctggc tttaacggca atataaacga aatagggccc ggccagaatg
    12421 cctgcattaa cttttctacg cgtggatcca aagtatatgt aaaatctttt aattgacctt
    12481 tactgtgtat atgaacaata agcggattgt cagatggacg gcctttagct tcatatattt
    12541 tagctacagc ttcttcatct gtcgcatttg ctgcaagtcc ataaactgtt tcagttggta
    12601 aacctattaa accaccgttt aaaacaatgt cttttatttc attaatttta ggatattgct
    12661 gtaaatcttc attatattct ctaacatccc aaattttagt atccaactta atcacgcctt
    12721 tcttatttat cataatataa agcaaaaagc tatgcactta actaatcata gcaaaggcat
    12781 aacttctaat taccatttaa atgagacgat tcgatcgtgg ccatttatat ctttaataat
    12841 gtcgattttt ttgtcaggaa atttatttaa aattattgat ttaagtgcct caccttgatt
    12901 gtaaccaatt tcaaaaacaa ctgggctgcc tttttccata acgtgaggta aatcttcaat
    12961 gattgattca taaatagcat atccatggtt atctgcaaac aatgcctgat gtggttcgaa
    13021 tctcgtaacc gttggagaca tcgtaaccat atctttttca tctatatatg gtggattaga
    13081 tatcaagccg ttcaacttga tatcttcatt aattaagggc tttaatgcat cccctgttaa
    13141 aaattgtatt tgtgattgat gcttctcagc attattacga gccatattca ttgcttcaag
    13201 tgaaatatca gtagcaataa catttaaatc cggcttttca catttcaaag taattgcaag
    13261 tacaccacta cccgttccga tatctacgat tgttgcatca tcttctaact gttgtaagaa
    13321 atgcaacatt acttcttcag tttcaggtct tggtatcaaa caatttgagt ttacatcaaa
    13381 cgttctacca taaaatgagg caaagccaac tatatactgt ataggctctc ctaataacat
    13441 acgttgtaat gctaagtcga acttcataat catcgctttc ggcatatcat catgcatgtg
    13501 gactacaaag tccgtacgcg tccattgaaa tacatctaac attaaccatt cagctcgtgt
    13561 ttgttcaaac cctttttgtt gtgttaaatg aattgcttca tctaactttt ctttataatt
    13621 caccattatt aagttctttc aatttatctg tctgctctga taaagtcagt gcatctataa
    13681 tttcttctaa atggccttcc ataatttgcc ctaatttttg aagcgttaga cctatacgat
    13741 ggtctgttac acggctttgt ggataattat aagttcgaat acgttctgaa cgatcaccag
    13801 taccgactgc tgatttacgt tgtgacgcat acttttgttg ttcttcttga actttcatat
    13861 cgtataaacg tgcttttaac actttcattg ctttttcacg gttttgaatt tgagacttct
    13921 cagaagatgt tgcaatgaca ccagttggta aatgggtaat acgtactgca gagtcagttg
    13981 tgtttacgtg ctgaccacct gcaccacttg aacgatacgt gtcgattttt aaatcttcat
    14041 ttctaatttc aatttctaca tcttcaactt ctggtaaaac tgccactgta gctgttgaag
    14101 tatgaatacg tccacctgat tctgtttcag gcacacgttg aacgcggtgc gcaccatttt
    14161 caaatttcaa tttactatac gcgccattac cagaaactga gaaactaatt tctttgtaac
    14221 caccatggtc actttcagac gcttctacta tttcagtttt gaatccttgt gattcagcat
    14281 actttgaata catacgcatt aaatcaccag caaaaatcgc agcctcatca ccacctgctg
    14341 ctgctcttat ttctacaata acgtctttgt catcattagg atctttagga atcaataata
    14401 ttttaagctc ttcttcaaga tttggaagtt cagctttaat accattactc tcctctttta
    14461 acatttctac ttcttcttta tcatcagtct cacttaacat ttcttcaata tcagctaatt
    14521 cttctttttt agctttatag ttacgataaa catctacagt tttttgtaaa tcagcttgct
    14581 ctttagaata tttacgtaat ttatctgaat catttacaac atctgggtca cttaacagtt
    14641 catttaactg ttcgtatctt tcttctacaa tatctaattg atcaaacact tataattcct
    14701 ccttattatt atcactaggt gctacgatat ggtgcgcgcg acaacgtggc tcataacttt
    14761 cattggcacc tactaagata atcggatcat cgattttagc tggtttacca tttattaatc
    14821 gttgcgttct actagatgaa gaaccacaaa cagcacaaac tgcttgaagt ttcgttactt
    14881 gttcactgac agccatcaat ttaggcattg gttcgaacgg ttcgccccta aaatccatat
    14941 ctaatccagc aacaataaca cggtgtccat ctgctgatag tttttctact atacttacaa
    15001 tttcatcgtc aaaaaattgc acttcgtcta ttcctataac atcaacatta gttaagtcgt
    15061 gcgtcataat ttcacttgct ttagaaatat taatcgcttc aatggcatta ccattatgag
    15121 agaccacttt ttctttatga tatcgatcat caatcgccgg tttaaataca acgacttttt
    15181 gtttagcgta tatacccctt cttagacgtc ttattagttc ttcggattta ccgctaaaca
    15241 tactacctgt aatacattct atccaaccgg aatggtaagt ttcatacatt gagagttcca
    15301 cctttttcaa aacataatcg ctttattata tcatatttca aatattcata aatgtctttt
    15361 tcataattat atcgatattg tacatgaaca attattttat aaatttgtct tgcatattta
    15421 tatacagcta ttctaatttg ttgcttctta ttcgtgcggt gtttctactc aatacaaact
    15481 ttaataaagg gggaaattta tcattatgta ttaacaacat ggtattgagt cacttctttg
    15541 aacacagtca taagtatata cccttactac gcttataaat aaaaaagctg tctacaattc
    15601 cattatagga tgcagacagc ttaacgatta ctatattagt tgtttgattt aagaccaaac
    15661 tttttgttga aacgttccac acgtccatcc gcagcagcga atttttgacg tccagtgtag
    15721 aatggatgtg aatcagatga aatatctaaa cgaataactg ggtattcttt tccatcttcc
    15781 cactccatca tttctgaaga tgtttttgta gaaccgctta agaatttgaa gtttgtagta
    15841 gtatctaaaa agattacttg gtgatactca ggatgaattc cttgtttcat tattttcagc
    15901 tcctttgccc tgaaccatct ggaacagagt tatttgtgag tttttaccca atactgtgta
    15961 attataaagg taattaattc aaaacgcaag cccctatata atgtttatta aattataggt
    16021 cgacccgttt tagtactttc ttctgcagac ttttgtagct gcttgaagaa atcttcatta
    16081 ttcttagacc ttttaagttt gcgaataaat ctttcagtaa agtcagttga gtcagtgaat
    16141 agatttctta attgccataa tgtgtctaat tcagatttac ttatcaacaa ttcttcttta
    16201 cgcgttgaac ttctgccaat ttcaattgca gggaagatac gacgttcaga caatttacga
    16261 tctaaatgta actccatgtt acctgttcct ttaaattctt cgtaaatcat atcgtccata
    16321 cgtgaacccg tatcaactaa tgcagttgca agtattgtta aacttccacc cgcttcaata
    16381 tttctcgctg caccgaagaa tgcttttggt ttgtgtaaag atgcaggatc taaaccacct
    16441 gataatgtac gaccacttgg tggaataact aagttataag cgcgtgctaa tctcgttata
    16501 gaatccatta aaataatgac atcttcccca atttctacta aacgctttgc acgttcaagt
    16561 aataattcag ctactttaac atggtgttct ggtggttcgt caaacgttga atgaacgact
    16621 tcagcagctt ctactgagcg ttctaaatct gttacctctt caggacgctc gccaactaac
    16681 aaaataaata gctttgcatc tggtttgttc gtactgattg cattcgctat ttcttttaat
    16741 aacgatgttt tacctgcttt aggtggcgcc actattaaac cacgttgacc taaaccaatc
    16801 ggtgttacta aatccatgat gcgcgttgaa taattttgtg tttctgtctc taatttaata
    16861 cgctcatctg gataaagtgg tgtcaaagct tggaaatgcg gacgtttctt cacttcttct
    16921 gcgttatggt cattgacaaa gtcaacttgt aataagccat aatatttttc gttatcttta
    16981 ggttttctaa ctttcccagt tactttatcc ccacgtttaa tttcaaaacg acgaatttgg
    17041 ctagcagata tataaatatc tttttcccct ttagaatagt tcactgttct taaaaaacca
    17101 taaccatctg gttgtatatc atctaagata ccttccatat aatagttacc atctttttcc
    17161 atttgtgctt ccataatagc tagaacaagt tcttttttat ttaatttact atagttcgtc
    17221 aacttaagag ttttagcttt ttgagtgagt tccttggtag tatagttctt gtacaattcg
    17281 tggaatgatt catactgagg agatgtacgt tctctttcag gcatatagtt tacacccatt
    17341 tcattatttt ttagttagta agtaatagat aggcatgaca atgcataaat aactgtgcta
    17401 gcacaatccc attatgctaa tccccaacct tttaaataca tgaataacta tagcaaattt
    17461 ttattttaat tacaaaatct atttataaaa attgcaaaat taaaacagta cacaaaactt
    17521 aattagtcat gtgcactgtt ttattgcgat ttttaattca aaaatcaaat ttatttaaaa
    17581 tatccagcta tagatttcac ttctaagaac tcttcaatac cataatcgcc ccattcacga
    17641 cctaaaccag attgtttata gccaccaaat ggtaaatctg gctttctacc tgcttcgttt
    17701 atttctactg tacctgcttc aatagaacga gctactttat gcaatgtttc tttgtcctta
    17761 ccaataacat atcctgccaa accatatttt gtatcatttg caatttgaat cgcttcatct
    17821 aaatcgttat aagtgataac tgacattact ggcccaaaaa tttcttcttg tgctatcgtc
    17881 atttgattat ccacattaat aaaaattgtc ggacgtgcaa agtatccttt ttcaagtcct
    17941 tctggtttac caggaccacc ataaaataat tcagcacctt cttcaatacc tttattaata
    18001 taattttgta cttgatcaaa ttgtttttta ctaatgatag ggcctacttg tgtaccatct
    18061 tctcttggat taccgacacg cacttggcta aattgttctt ttaattcagc taagaatgca
    18121 tctttaattt ttttaggcac taaaactcgt gtaccagctg tacatacttg accagtatta
    18181 ttaacaactt tgcctgttgt tgctttagcc gcttctttaa tatctacgtc atctaggacg
    18241 atgtatggtg atttgccacc aagctctaat gatacctttt taaaatcttt agcagctttt
    18301 tccataattt tagaaccagt agggcctgat cctgtaaatg acatcatgcg tactttagga
    18361 tgctcagata aaggattccc aacgccagca ccatcaccat taacaaggtt aaatacacct
    18421 ttaggaacac cgactttatc aaaaatctca gctaaaataa cagctgcaaa tggtgtttct
    18481 tcagatggtt taagtacaac tggactacca gccgcaaatg ctgctgctaa ttttaatgat
    18541 gtttggtttg tagggaagtt ccacggtgta attaagccag atacaccgat tgcttcttta
    18601 acaactaaat catctccgcg gcgttcttca aattcgtagt tatctaatgc gtctctcgct
    18661 gcaacaaaat ggtttagtcc catttgataa tggacacgct cagataatga taaaggagca
    18721 cctaattcat ccgtaatagc ttgtacaata tcgtcttttc tgttttcata ttcttttaca
    18781 attttatcta ataacgcttg tctttctttc acagatgtat gacggaactc taaataaaca
    18841 ttgtctgccg cctcgacagc tttatcaaca tcagctttat tgcctttagc aactttcccg
    18901 attacttctt cggttgctgg atttataact tctatcgttt cattactatt actttctacc
    18961 cattcgccat taatgtattg ctttgtgtag tctctcatta ttctttcact cctcaagaat
    19021 gatttatata ttatttatcc gtaatgcata cgatataaac attacattcc attatgatac
    19081 gctccctttt aatatgttca aaataaaatg ctttaaaatt ttgctgcatc tattttttta
    19141 tatttttaaa taaaaataac tattcccaca tcatcgcaaa taatgcgaag acttagaaat
    19201 agttataatc aatgctatgt ttaaacgtca tgtaattatt tagcagtacg ttgatctgtt
    19261 aaatcgacat atgattgcgc ccatgcttca attggatgta aagcctctgc taacgcttta
    19321 cctttttcag ttagcacata aataatttgt actggactcg tagaaatgat ttgcttctca
    19381 actaactccc attgtgcaag ctctgacaac ttaagactta aagcacgtgg tgttattgtt
    19441 ttcaaatctc ttttcatatc ggaaaagtgt gctgaacagt cattacatct tgagagataa
    19501 ttaatgatta atccattcca acttctacca agtattttaa aagtttcttc gagatacgga
    19561 catacttcca tcatcttcac ctctcatcaa taaatttaga atgtaaacga acaccctttt
    19621 ttaatcagta taattattat accacaaaac aagataaatc tatacagttt ccgtccaaat
    19681 atctgcacct aaagctttta agtgttctac aatatctgta taacctctat aaatatgttt
    19741 tacattgtaa attgtagtta caccctcagc aattaaacca gcaataatta aacaagctcc
    19801 tgctcttaaa tcgctagcat aaacttcagc accatgtaat gttgatggct ttatcgttgc
    19861 tgtgccttcg tcaacttcaa tatttgcacc catgcgcttt aattcttcaa catgtttaaa
    19921 acgctccgga taaatcgtat cagttacaaa tgaaggacca tttgccataa ataataatgg
    19981 tgtaataggc tgttgcaaat cagtagcaaa acctggatat actagtgttt taatatcaac
    20041 aaattgatat ggcgcattat tattgatgcg aattctttcg tctcttacat caacattcac
    20101 acctaattca ctaaatttag cagttaatgt ttctacatgt ttcggaacaa tattatttaa
    20161 tataacattt tctccacatg ctgcagcgat acacatatat gtgcctgctt caattctatc
    20221 aggtataact tgatactcag aaccatgtaa ttctttgacg ccattgattt taattgttga
    20281 tgtacccgct cccttaatat tagctcccat acttgttaag aagttagcaa catcaactac
    20341 ttccggttct ttagcagcat tttcaattac agtttgtcct gttgcataaa ctgcagctag
    20401 cataatgtta attgttgcac ctacgctaac catatctaga aaaatatgtg caccttttaa
    20461 ctctttagct tcgattttca ttgatgtcgt acttgattca tcaatttcag cacctaaagc
    20521 tttaaatcct ttaatatgtt gatcaattgg acgtggccca agtggacatc ctcccggtaa
    20581 accaatcaca cattttttaa atctacctaa catggcaccc atcatatagt atgaagcacg
    20641 taatgattca actttattat ttggtaatgc agcattttgt atttcagttg tatcaacttc
    20701 taattccata ccatttagtg atgccttaat atttaaatct tctaataaac tcactaaagt
    20761 tttaacatca gagatttgcg gtaacccttc taatttcaca tgtccttgag ctaataatgt
    20821 tgcaggaatg atagctacag cactgttttt cgcgccactg atattaactt ccccatttag
    20881 tgtgcgtcca cctcttattt ttattacctc ttgagccata tcatttatct cctttgtcca
    20941 ttacttttta attttttgca ttattagcat tataaattaa ttttacttta tttgtaaaat
    21001 agtttatttt aatcgttaaa atcttgtctt tctattttcg attgattaat aacaaatcat
    21061 tcacctttac taatatatgc tgttcttccc cacttgacca tggtctaaag tctgattatt
    21121 caactaaata ttgacgttgt tttgacaatt atttagtttt aattctacgg ttttcacgta
    21181 aacttttttc ataaattgct cattttcctt ataattatat cacaatcaat tttgatatat
    21241 tttaccattt ttacgctttc attttaaaat caaactgttt tatttattac ttatacaaca
    21301 taaaaaagaa gcaaacacca ttttgtctgc ttcttttaaa attaatatcc ctacgttatt
    21361 aataacttaa agactaaata ttaattattt agcgcggttt gaagtaccga actctttaat
    21421 tttaccttta actgtttctt tgatagcttc acgtgcaggt cctaagtatt tacgaggatc
    21481 gtaaacttct ttgtcgttat ttaaaacgtc acgaactgct tttgctgaag cgatttggtt
    21541 ttcagtgttt acgttaattt tagctgtacc aaatggaatt gctttttgga tatctttagt
    21601 tgggatacca gtaccaccgt gtaatactaa tggtaaacca gttgataaac cgatttcttc
    21661 catttctttg aatcctaatt ttggttcacc tttgtatgga ccatgaactg aacctaatgc
    21721 tggcgctaat gcatcgatac cagttttttc aactagttct tgacattctt taggatcagc
    21781 ataaatgatg ccgtctgcta caacatcatc ttcttgtcca ccaacagtac ctaattcagc
    21841 ttctacagaa acaccttttt catgagcgta ttcaacaact tttttagttg ttgctacgtt
    21901 ttcttcgaat gggctgtgtg aagcatcgat cattactgat gtgaaaccag catcgatagc
    21961 ttctttacat ttttcaaagc ttgaaccatg gtctaaatgg attgctacag gaatagtgat
    22021 gtttaagtca tgcattaacc cttcaaccat tttaacaatt gtgtagaaac cgctcatgta
    22081 acgagcagca ccttcagaaa cacctaaaat tacaggtgcg ttttcttctt gtgacgcttc
    22141 taaaattgct tgagtgaatt ctaggttatt aatattgtat tgacctaccg cataaccatt
    22201 ttcttttgca tcaattaaca tttctttcat tgaaactaaa ggcatgaaag ttcctccttg
    22261 agtgcttacg cacgtatttt tcaaagtaat tatatcaaaa aaatagcgtc aatgcattga
    22321 aatactgcag attaaatgtg tattttttga aaaatgcgat agttatacta aatgcaaacg
    22381 tatacatttt tatacaattt aagccgttgc ataatatgta gtcattaatt acaatatttt
    22441 ataagtagat tggaggataa agatgtcaaa aatcttaaac acacaactaa taggtatctt
    22501 taataggcta gaaaaacaat ctttagaaat tcaaatggcc gcccaatgtt taattcaagc
    22561 aattggtggc gaaggttatg tttatgtaaa aggttatgat gatttacagt ttttcgaaag
    22621 tttcattcta catagtgatg agcgtttaaa atctagccga aaacttgacg ctattaaaga
    22681 ctttaaagaa attgactcaa ctgatcgtgt attattattc gcaccatttt ataatgatca
    22741 agttgcatta gatattcaaa aacttatcga cttagatatc gatgtggtct taataagcaa
    22801 caaacctaaa acagatgatt tccctgatca tctcgtacat tttatcgatt tatctacacc
    22861 aagacctata gtctacactg aagattacga taagattgtc caaccacatg cgatggcatt
    22921 gaattatgta tactatgaca tttatacaca aatgattgaa atgacaagag acttagaatt
    22981 ataaaagtca ctattgttat ttcatacctt gtcactaaca taatattaaa attatatgaa
    23041 aaaccggtac tttcaatgtt taacaatgaa agtaccggtt ttattagcaa atttatttat
    23101 tttgttgata ttttaacgaa gcttcaataa atgatttaaa aatcgggtgc ggacgatttg
    23161 gtctagataa gaattctggg tggaattgac aagcaataaa gaaatcattt gtcggaatct
    23221 ctaccatttc tactaaacgt ccatctggac ttgtaccaga aattaccata ccatttgctt
    23281 ctaattgttc tctatagtca ttattaaatt cataacgatg acgatgtctt tcttcaattt
    23341 ccgctttacc ataaacatct tgtgccaatg tgccttcttt aattgaacat ggatataagc
    23401 ctaagcgtaa tgtaccacct aaatcttcga tatctttttg ttctggtaat aaatctataa
    23461 ttgggtatgg tgttgctggg tctaattcag ctgaatgtgc gccttcaagg cctaatacgt
    23521 ttcttgaaaa ttcaactgtt gcaagttgca ttcctaaaca aataccaaag aatggtacat
    23581 tgttttctct agcatactta attgcactaa ttttaccttc acttgcacgg aaaccaaatc
    23641 cacctggtac taaaataccg tcgacatctg caaggtattc ggctgcattt tcatctgtta
    23701 cttcacttga atcaatccat ctaatgtcaa tatctttggc aaaaggatat ccagcatgtt
    23761 tcaatgattc aacaactgat aaatatgcat cttgtaagct aacatattta cctactaaac
    23821 caattgtaat tttaccatct aaattattaa cgatatctaa caactgtttc cattcatcaa
    23881 gctgtgtttc atatttcgcg tttaattgta aacgtttaat aacgatatca tccatatttt
    23941 gttggcttaa ttgtaatgga atttcgtata aagagtctgc atcacgacat tcaataacac
    24001 tttctttatt aatgtcacag aataatgcaa ttttatcttt taaatcttgt gtcatttcat
    24061 attcagttct tacaacgatt aagtctggtt gaatacctaa gcctcgtaat tctttaacac
    24121 tatgttgtgt tggcttcgtt ttcatttctc cagcagcttt aatataaggc agtaatgtac
    24181 agtgaacata cataacattt tctctaccta aatcgctacg aatttgacga atcgcttcaa
    24241 taaacggtaa tgactcaata tcacctgttg taccgccaat ttcagtgata acaacgtctg
    24301 cattcgtact ttcccctgca agtaataaac gttctttaat ttcatttgta atatgcggaa
    24361 taacttgaac tgttccacct aagtaatcac cacgacgttc ttttttcaat acgtgtgaat
    24421 acactttacc ggctgtcaca tttgaaaact tgtttaaatt aatatcgata aatctttcgt
    24481 aatgtcctaa gtctaggtca gtttctgcac catcatccgt tacgaatact tcaccatgtt
    24541 gataaggact cattgtacct gggtcaacat ttaagtatgg atcgaatttt tgaattgtta
    24601 catttagacc tctatctttt aataatctac ctagagaaga tgctgtaata cccttcccta
    24661 atgatgaaac tacgccacct gttacaaaaa taaattttgt catgtcttga cctcctaatt
    24721 attaaaggga atgcttcctc atcaattgta aaaattacgt aaaacataac attaaagcaa
    24781 gctcattcaa gttgtttatt tgtcgaattc gacaaaatta aattctgttt acttaccgtt
    24841 tccatactgc tatgtcacta tgtatatcat ttacaacttt gatttttgcg ttatcatttc
    24901 ttgatttact caattaaata aaaaaacgct ccctctcaca aattctgtaa ggggagcgta
    24961 taaattttat acacgtgtcc tatttaaagg agcccgaata aaattttatc atctttcaac
    25021 taaaagtcaa acaaaaaatt aatcgttgaa gtcttcttcg tcttcaaaca cttcttcgtc
    25081 ttcgtctagt tcatcttcat cttcttcaat gactatatcc gaatgattga tttcttcttc
    25141 aacctgctca tcttctggat catttagttc ttcttgatca tctgtttgag ctggaatatc
    25201 atcgtcgtca tccatttcat cttcgcccaa taattttaag ttttgatctt cttcatcatc
    25261 tgcatccaga atatcgaatt tttgaatagt tggtgcgatt ttctcttcaa tatcatctac
    25321 cgaataccaa tcacgtaatc cccataaatt ttctccaaca tttaaaaaac gaccatctgt
    25381 gtttaaatcc gtgtaaaatt gtacaacacg attttcaatt tcttcgtact cataatcacc
    25441 taacgctcta aattcatcga tgatatcata taagttcatt gtttcgcctt tatcattcaa
    25501 taatgtataa gccatatcaa taaatgattt ttcatcaacc atttgttttg tataatcttg
    25561 aattttcatt gcccaatact tcctttcgaa ggatataatc taaatttata cgtctgaatt
    25621 catttatata tagtaacaaa actaaattat aaatgacaat atcatatttc gcaatcactg
    25681 ttagtaattt aaatattttt caaaaacgac ataatcatca gtttgttcga tttcaacgaa
    25741 attactattt agacataatg gtttaaaatc attattctct ccaaacagaa tcatcgtaat
    25801 cacattaagg ttgtcatagt tttttcttaa gtaagttggc aattgtcgca gcgcacttga
    25861 accaatacca ttaaagcgat atgatttatc cacttcaata gcagcaatgt tcatgcgttc
    25921 accaatctgc tccaatgcga taaagccaac cttttggcca tcaatcatta aattaatgat
    25981 atgtacattg gtcacttctg aacgactttc ttcttcaagc tttaatgacg aaacatgttt
    26041 tgaatctaag tccaagtaat atagtcgttc tttgccaatt ggtccttctt ctttagtcgc
    26101 tgtatgcatg aatccagcac gttcgtaaag gttccaagca ttgtcatttt ccgcatctac
    26161 tactagatat agatgattaa aatcaggaaa tacaccttga acatattgcg gcaatgacat
    26221 cattattttc gtgccatatc caaaaccttg atatttttca ttaatcgata atgaacgaat
    26281 ataaacgaca ttttcaggtg tatcataacc ttcatgctga tagtaacgat gtaatacaaa
    26341 aaagcccacc acttcatttt tatcgtttat tgctacgtta gcaatcctat cagcatcatt
    26401 aattgcatca tcaataactt ctttaggtaa agatgaatat atttgttgtc tttcattcaa
    26461 gtcaaaatca ttaattgctg aacgatattg ttcatcaaat gcttttaaag tgataccttc
    26521 aaaacaaagt tctttagcca tatatttaac ccctatcttt ttatatatga agcatgaatc
    26581 cccatattat tttacctatt atttcactac gataccatta tttgctctaa gttaaataaa
    26641 ttacttattt atattttttg aattttaaaa ttaatgtgct actcctcatt acttatcatt
    26701 ttaatcagag acttacaaat acactgcgta cgaactactt ttaatattgc gtcactaact
    26761 aacaatccta tacaacttca aaattcctag aaaataatat atataactta acgtctacgt
    26821 ttgtaataca ttaattgcga tacgcttaca atgttgaaat acaatggcat caaatttgtt
    26881 aataattact attattactt gttttttagt cattttacta tactattatt taaggataga
    26941 cattaataat gaggtgggaa tcttatgaaa gttggcattg acgctggcgg tacactaatt
    27001 aaaatcgttc aagagcaaga taaccaacgt acttttaaaa ctgaattaac taaaaatatt
    27061 gatcaagttg tggaatggtt aaaccaacag caaattgaaa aattatgctt aactggaggt
    27121 aatgcaggtg tcattgctga aaacatcaac attcctgcac aaatttttgt agagtttgat
    27181 gctgcatctc aaggactagg tattttgtta aaagagcaag gccatgactt agcagattat
    27241 atttttgcaa atgttggtac cggtacgtca ctacattatt tcgacggcca atcacaacgt
    27301 cgtgtaggcg gtatcggtac aggtggaggc atgattcaag gactcggtta tttactaagt
    27361 caaatcactg attataaaca gttaactgac atggcacaac acggtgatcg taacactatc
    27421 gatttaaagg ttagacatat ttataaagat accgaaccac ccattcccgg tgatttaaca
    27481 gcagctaatt ttggtcatgt actacatcat ttagatgctg attttacacc tagcaataaa
    27541 ttagcagccg ttattggtgt cgttggcgaa gttgttacga caatggccat tactgttgca
    27601 cgtgaattta aaactgaaaa tatcgtttat atcggttctt catttcataa taacgcttta
    27661 ttacgcaaag ttgtggaaga ttatactgtg ttacgcggtt gcaaaccgta ctatgttgaa
    27721 aacggcgcat tttcaggtgc tattggtgca ttatatttag aaaaatagaa aaagaccatt
    27781 tatatgcgcg ttgttttaaa taaacgctat ataaatggcc ttttatttta tggtctaagt
    27841 gttttgaata cgtattgttc catttattat cacttaatta cttacaactc aattcaatta
    27901 aaatgtaagt tcattagaca atttttgcgc tgaaacaatt gtaccaacca cgccagtttt
    27961 taaatctatc gcaacggctt tatgatcatc tctaagttca tctagccatt ctccgaaaac
    28021 agaaccttca atcgtaatta aatcacactc ttcaaaatct tcatctcgaa cttttaacgc
    28081 tctgttttta atactccata caggcataat atgcggcttt ttttcgtcgc tatcattttc
    28141 actaatgaga ataaactgct tactaccttt tttagttaag ccgtatactt catcatattt
    28201 cgctacatct tggacaaatt ctttgattct aattttatct aattccgtca tcacttcttg
    28261 tgtaacagct acaacgttta gtagatgccc ttcttcttcc attgtttgat tcataataat
    28321 atggtcttgt tcatcaaaca attcatctaa ttcataagta gcaaaacgat caacgtccat
    28381 ttttacaact ttatcaaatg gaatatcacg acttgttaaa aatgatacgg ccacactttc
    28441 ttctgtccaa acgcctacat acgcttcact tttataattt aggcgtacta gttttttatt
    28501 tttagaggcg aaataaatac attcatttat taatatatct ctaaagaatg gatatgcttt
    28561 ataagacatg cttgaccctc actttttaaa atatgtcata cataaaatag taaatgactc
    28621 caacaagtta gtcaattaga catattatga attcaattta atgtcatgtt tttcatttta
    28681 aaaattcaat agcattaaat tttacgtata cacaaattcg gtctataaca cgaaacatat
    28741 ctagttacta aaaatttcaa aacgataacc tttagtatac ctttatcaac aaaatggcaa
    28801 ctcttataac atagtgaaat aaaagtcgcc atttaatatt agtattacaa agtattgcca
    28861 tatcataaat ttaattactg ttgactagaa tcgaaaaaag cttgttacaa acgcatattc
    28921 agtcaactac tggcaacaca acattgtagc gccttatgtt ccgaactcta atagtgttca
    28981 agttcagcta aaaatgcgcg ttcattctcc agtactgttt cttttgaatc accatgccaa
    29041 atacagccaa accttgattt aactttatct tcaccgtacc aatcaccatc ataataagat
    29101 aacgggtata aactaccttc tttgacatat ttcaaaatcg tattgaagaa atgtgtgtta
    29161 tcaccttttg aatgataact ataaaacttt tgatatcctg aattcaactc gttagcaagt
    29221 aataacatgc tcgttgaacc attttgtcta aagtttaaat caatcgcata aacgttatca
    29281 tcctcatcga cgagtaggtc aaaaccagct acaccaaaga atccttgatt cacaccgttt
    29341 tccataattt gtctacctgc ttctataacg tgctctggta cattagtagt attttcattt
    29401 ccgttataaa atccgtactt atccgttaat tgcgttgccg caccaagata ttgaatgcct
    29461 agcgattccg agtatgcaaa ctgaacgcaa taattcgctt tttcttcaat cttctgttcg
    29521 ataataagcg aattcgtttc agcagttgcc tctttaatac gcgttatcgc tttttgtaaa
    29581 tctgcatcat gataacaaat cataacgcca tagccacctg ctgtcggaag atcatctccc
    29641 ggcttaatta ctaacgggaa ttcccaattc ttaatctcgt tttcgaattg ctcaattttt
    29701 acaacttttc tttttggtaa aaacttccca tttgtccatt caggtattct tgctttatta
    29761 tttaaagcaa caaataacgt tttatctaat gcataatatt gctgattcaa gattgtttca
    29821 tcatgaatat attgaaaata aatcttttta ttttccttat gtgccaattg tttgatcaag
    29881 ttttcgtaag attgctgatt gttaaatgta taaattgagt tcggtacttc cttaccaata
    29941 acttgaaata gctgatgcaa tttgtctgtc gcactagctt cgtgaacaat aacaggtaat
    30001 tgatttgcta ttaataactc cctaccagtt aaaaaattag attgatgttc gtccggtttc
    30061 aaccatggat tcgatatata cgaaggtctt gacgtataga caacatcttt gtcatataaa
    30121 tcacttaacg ttaagttcgg ctcattacca ttatttgtca ttacttccca ttccctttca
    30181 aatgcgcatg ctcttcaata atgtcttgat aaacgtcttg atttgtaatt aactctaacc
    30241 ccatcaacgc cattatttta gcgcctttaa ttaatgcttc atcaccatgt acactcgcag
    30301 ccgcttctct aaatctatgc gtatgtccta ctaaattacg tgatcctatt ttaatatgag
    30361 gatgtattgt tggcacaaca tgacttacgt tccctgtatc cgtagagcca taaccaaaat
    30421 catcatcaat aactgcttca ccaacttctt cagcatattt agcaaataaa tcatctaatt
    30481 tcggcgtttt aatgaattca ttcacaccgt tttgaattcg accaaattca taatcacaac
    30541 cagtctgtat cgcagctcca cgtgcgattt gatttacttt ttctgttaat atatccaatt
    30601 ctttacgcgt cattgctcta gtataaaaac gagcatgtgt atagtctgga ataatattag
    30661 ctgctttccc gccatctaaa atcacaccat gcacacgttg atctttttta atatgttgtc
    30721 gtagttgtgc tacaccatta aaataactaa tcatagcgtc taatgcattt aacgcttcat
    30781 ctgcattttc agaggcatga gcactttttc cgtaaaattt aacatctaaa acatccactg
    30841 ccaaagtatc aatcgtttta taagtttcat ttcccggatg aatcattaag gcaatgtcta
    30901 tttgatcaat cacaccagcc ttgacataag aagctttagc gctaccattt tccccacctt
    30961 cttcagctgg acatccaaga acgactactt taccaccaat ttggtcaatc acttgcttca
    31021 aaccaattgc accaagcaca cttgcagttc caatgatatt atgaccacaa gcatgaccca
    31081 accctggcaa agcatcgtat tctgctaaaa aacctatagc tggcccgtca agacctgaat
    31141 catatgtcgc tataaaccca gttgcatgcc cagcaatctc ggtttcaatt tcaaaatcat
    31201 gctctttcaa acgatctatt aaagttcgag acgcaaatat ttcttcatta ccaagttcag
    31261 gacgttcatg aattctatga ctgatttcga tataactata tttatttgtt tctatataat
    31321 cgagaatttg ttgtttttca ctcatttttt actatctccc ttttacccta cactcatttt
    31381 aatcatccac aatattttgt tctttcaaaa tgaattatta cttattctat cggttttatc
    31441 tcatgatgtc atctagtttt tc
//
LOCUS       DAMZKC010000013        30295 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.013, whole genome shotgun sequence.
ACCESSION   DAMZKC010000013 DAMZKC010000000
VERSION     DAMZKC010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 30295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30295
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.013"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            81..890
                     /locus_tag="POB55_000432"
     CDS             81..890
                     /locus_tag="POB55_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HDI6499428.1"
                     /translation="MDFSNFFQNLSTLKIVTSILDLLIVWYVLYLLITVFKGTKAIQL
                     LKGILVIVIGQQISMILNLTATSKLFDIVIQWGVLALIVIFQPEIRRALEQLGRGSFL
                     KRYTSNTYSKDEEKLIQSVSKAVQYMAKRRIGALIVFEKETGLQDYIETGIAMDSNIS
                     QELLINVFIPNTPLHDGAMIIQGTKIAAAASYLPLSDSPKISKSLGTRHRAAVGISEV
                     SDAFTVIVSEETGDISVTFDGKLRRDISNEIFEELLAEHWFGTRFQKKGVK"
     gene            892..1824
                     /locus_tag="POB55_000433"
     CDS             892..1824
                     /locus_tag="POB55_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HDI6499429.1"
                     /translation="MLESKWGLRFIAFLLALFFFLSVNNVFGNIFNTGNLGQKSSKTI
                     QDVPVEILYNTKDLHLTKAPETVNVTISGPQSKIIKIENPEDLRVVIDLSNAKAGKYQ
                     EKYQVKGLADDIHYSVKPKLANITLENKVTKKMTVQPDVSQSDIDPLYKITKQEVSPQ
                     TVKVTGGEEQLNDIAYLKATFKTNKKINGDTKDVAEVTAFDKKLNKLNVSIQPNEVNL
                     QVKVEPFSKKVKVNVKQKGSLADDKELSSIDLEDKEIEIFGSRDDLQNISEVDAEVDL
                     DGISESTEKTVKINLPEHVTKAQPSETKAYINVK"
     gene            1851..3206
                     /locus_tag="POB55_000434"
     CDS             1851..3206
                     /locus_tag="POB55_000434"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HDI6499430.1"
                     /translation="MGKYFGTDGVRGVANQELTPELAFKLGRYGGYVLAHNKGEKHPR
                     VLVGRDTRVSGEMLESALIAGLISIGAEVMRLGIISTPGVAYLTRDMGAELGVMISAS
                     HNPVADNGIKFFGSDGFKLSDEQENEIEALLDQENPELPRPVGNDIVHYSDYFEGAQK
                     YLSYLKSTVDVNFEGLKIVLDGANGSTSSLAPFLFGDLEADTETIGCSPDGYNINEKC
                     GSTHPEKLAEKVVETESDFGLAFDGDGDRIIAVDENGQIVDGDQIMFIIGQEMHKNQE
                     LNNDMIVSTVMSNLGFYKALEQEGIKSNKTKVGDRYVVEEMRRGNYNLGGEQSGHIVM
                     MDYNTTGDGLLTGIQLASVIKMTGKSLSELAGQMKKYPQSLINVRVTDKYRVEENVDV
                     KEVMTKVEVEMNGEGRILVRPSGTEPLVRVMVEAATDEDAERFAQQIADVVQDKMGLD
                     K"
     gene            3492..10997
                     /locus_tag="POB55_000435"
                     /pseudo
     CDS             3492..10997
                     /locus_tag="POB55_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500880.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
     gene            complement(11317..12423)
                     /locus_tag="POB55_000436"
     CDS             complement(11317..12423)
                     /locus_tag="POB55_000436"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDI6499431.1"
                     /translation="MKAVHFGAGNIGRGFIGYILADNNVKVTFADVNEEIINALAHDH
                     QYDVILADESKTTTRVNNVDAINSMQPSEALKQAILEADIITTAVGVNILPIIAKSFA
                     PFLKEKTNHVNIVACENAIMATDTLKKAVLDITGPLGNNIHFANSAVDRIVPLQKNEN
                     ILDVMVEPFYEWVVEKDAWYGPELNHIKYVDDLTPYIERKLLTVNTGHAYLAYAGKFA
                     GKATVLDAVKDSSIEAGLRRVLAETSQYITNEFDFTEAEQAGYVEKIIDRFNNSYLSD
                     EVTRVGRGTLRKIGPKDRIIKPLTYLYNKDLERTGLLNTAALLLKYDDTADQETVEKN
                     NYIKEHGLKAFLSEYAKVDDGLADEIIEAYNSLS"
     gene            complement(12423..12857)
                     /locus_tag="POB55_000437"
     CDS             complement(12423..12857)
                     /locus_tag="POB55_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDI6499432.1"
                     /translation="MSELFSNDNIFLNVNVNSQNEAIEKAGKALVDSGAVTDAYIQAM
                     KDREQVVSTFMGNGLAIPHGTDEAKTNVIHSGLTLLQIPEGVDWDGEVVKVVVGIAGK
                     DGEHLDLLSKIAITFSEEENVDRIVQAKSAEEIKQVFEEADA"
     gene            complement(12869..15001)
                     /locus_tag="POB55_000438"
     CDS             complement(12869..15001)
                     /locus_tag="POB55_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDI6499433.1"
                     /translation="MLLSTREKEMIALLIKYHGQYITIHDIAQQLAVSSRTIHRELKG
                     VEAYLTSFSLTLERANKKGLRIAGTDSDLNDLKQSIAQHQTIDLSVEEQKVIIIYALI
                     QAKEPVKQYSLAQEIGVSVQTLAKMLDDLELDLNKYQLSLSRKRGEGIYLVGTESKKR
                     EFLSQLMVNNLNSTSVYSVIENHFVFHSLNQIHKDFVDLERIFNVERLLMDYLSALPY
                     QLTESSYLTLTVHIVLSISRIKNGEYVALNDDIYDSVQNTFEHKVASELADKLGQIYD
                     VTFNQAEIAFITIHLRGAKRKNLNDTSLNNRCEENKIKAFVNKVEMISGMTFADLDTL
                     VDGLTLHLNPAINRLQANIETYNPLTDMIKFKYPRLFENVRLALNDCWPDLIFPESEI
                     AFIVLHFGGSIKNQGNRFLNILVVCSSGMGTSRLLSTRLEQVFSEIERITQASVSDLK
                     SLDLSQYDGIISTVNLDIDSPYLTVNPLLPDSDISYVAQFLNTKSTFQETHDKSSNMI
                     DKDDVHVETKDIDGNTSFENEQTSYLTSVFEKHLSDEKSEQLMHHMRSGLTLLDSVKI
                     VSTEVKQWQTYIADYLYQCDAINDPTSFAELLEQRLIDNPGWILSPYPVAIPHLRDNM
                     IKHPMVLITVLEEPLILPSIQNDNQTIKYMISMFIPDNDFMASLVSDLSEFLSLKLES
                     IDTFMENPQELETLLRNKFLERIKKQFI"
     gene            complement(15036..16574)
                     /locus_tag="POB55_000439"
     CDS             complement(15036..16574)
                     /locus_tag="POB55_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HDI6499434.1"
                     /translation="MSQTEEKKGIGRRVQAFGSFLSSMIMPNIGAFIAWGFIAAIFID
                     NGWLPNKDLATLAGPMITYLIPLLIAFSGGRLIYDLRGGIIAATATMGVIVALPDTPM
                     LLGAMIMGPLVGWLMKKTDQLIQPRTPQGFEMLFNNFSAGILGFIMTIAGFKILAPLM
                     KFIMHILSVAVEALVHAHLLPLVSILVEPAKIVFLNNAINHGVFTPLGADQAAKAGQS
                     ILYTIESNPGPGLGILLAYMIFGKGTAKATSYGAGIIHFLGGIHEIYFPYVLMRPLLF
                     IAVILGGMTGVATYQATGFGFKSPASPGSFIVYCLNAPRGEFLHMLLGVFLAALVSFV
                     VAALIMKFTREPKQDLEAATAQMENTKGKKSSVASKLVSSDKNVNTEENASGNVSETS
                     SSDDDPEALLDNYNTEDVDAHNYNNINHVIFACDAGMGSSAMGASMLRNKFKKAGIND
                     ITVTNTAINQLPKDAQLVITQKKLTDRAIKQTPNAIHISVDNFLNSPRYEELLNNLKK
                     DDQA"
     gene            17255..19060
                     /gene="glmS"
                     /locus_tag="POB55_000440"
     CDS             17255..19060
                     /gene="glmS"
                     /locus_tag="POB55_000440"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HDI6499435.1"
                     /translation="MCGIVGYIGYDNAKELLLKGLEKLEYRGYDSAGIAVVNDDNTTV
                     FKEKGRIAELRKVADSSDFDGPVGIGHTRWATHGVPNHENSHPHQSSNGRFTLVHNGV
                     IENYEELKGEYLQGVSFISETDTEVIVQLVEYFSNQGLSTEEAFTKVVSLLHGSYALG
                     LLDAEDKDTIYVAKNKSPLLLGVGEGFNVIASDALAMLQVTSEYKEIHDHEIVIVKKD
                     EVIIKDADGNVVERDSYIAEIDASDAEKGVYAHYMLKEIHEQPAVMRRIIQEYQDAEG
                     NLKIDQDIINDVKEADRIYVIAAGTSYHAGLVGKEFLEKWAGVPTEVHVASEFVYNMP
                     LLSEKPLFVYISQSGETADSRAVLVETNKLGHKSLTITNVAGSTLSREADHTLLLHAG
                     PEIAVASTKAYTAQIAVLSILSQIVAKEHGREADIDLLRELAKVTTAIEAIVDDAPIM
                     EQIATDFLETTRNAFFIGRTIDYNVSLEGALKLKEISYIQAEGFAGGELKHGTIALIE
                     EGTPVVGLATQEKVNLSIRGNVKEVVARGAHPCIISMEGLEKEGDTYVIPHVHELLTP
                     LVSVVALQLISYYAALHRDLDVDKPRNLAKSVTVE"
     gene            19292..20074
                     /locus_tag="POB55_000441"
     CDS             19292..20074
                     /locus_tag="POB55_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6499436.1"
                     /translation="MLIQLDQIGRMKQGKTILKKISWQIAKGDKWILYGLNGAGKTTL
                     LNILNAYEPATSGTVNLFGKMPGKVGYSAETVRQHIGFVSHSLLEKFQEGERVIDVVI
                     SGAFKSIGVYQDIDEEVRNEAHQLLKLVGMSAKAQQYIGYLSTGEKQRVMIARALMGQ
                     PQVLILDEPAAGLDFIARESLLSILDSLSDSYPMLAMIYVTHFIEEITSNFTKILLLK
                     DGESVQQGLIDDILTSENMSRFFQKNVAVQRWNNRFSMAMLE"
     gene            20142..20999
                     /locus_tag="POB55_000442"
     CDS             20142..20999
                     /locus_tag="POB55_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDI6499437.1"
                     /translation="MDNVKAIFLDMDGTILHENNQASPYTKDVINRLREKGYKVFLAT
                     GRSHSEIHQLVPQDFAVNGIISSNGTIGEVDGEIIFKHGLSLAQVQQITNLAKRQQIY
                     YEVFPFEGNRVSLKEDETWMRDMIRSQDPINGVSHSEWSSRQDALAGKIDWVTKFPEG
                     EYSKIYLFSSNLEKITAFRDELKQNHVQLQISVSNSSRFNAETMAYQTDKGTGIKEMI
                     AHFGIHQEETLVIGDSDNDRAMFEFGHYTVAMKNARPEIQALTSDVTAYTNEEDGAAK
                     YLAKHFLAD"
     gene            complement(21658..21816)
                     /locus_tag="POB55_000443"
     CDS             complement(21658..21816)
                     /locus_tag="POB55_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001792784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HDI6499438.1"
                     /translation="MSKSSDALFNSLTQSFELIEGVYNELVTDNLKIVMYKPRKESTS
                     GRINRRFK"
     gene            22496..22600
                     /locus_tag="POB55_000444"
     CDS             22496..22600
                     /locus_tag="POB55_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499439.1"
                     /translation="MLLLERTSMSDFEMLMVVLTIIGLVLISTQDHKK"
     gene            complement(23033..24124)
                     /locus_tag="POB55_000445"
     CDS             complement(23033..24124)
                     /locus_tag="POB55_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDI6499440.1"
                     /translation="MFTYFKSAFKNAKPQLLITLIYALIAFAVIAVVYLLANFQLAKY
                     VQTIAIYSQFGQKPPVDAYLKVIAVLLIAAVVSLFVLVQIFIGITNVMKRAMSHEKVK
                     FTDLFIAFKKGNYLKSVLIGLVSIAMIIVLSLLTSLLYKLFSPVSEMIMNSVQSSYAD
                     STHLIGIAITTQSIIIIVVLLIKAIITWLLLIPVFNFMTSFVESTNDKVKTHLANGFK
                     AMKNGQKTFFKFFIGILLLNLIIILFKTPVGYLISFNTQSLSQSVAENIIRVYTVMTI
                     ILFVVIHAIILMGIVQYYLKRGQKITKDKVKTADKNKKVVTEPKNTKVENDKVTTSVE
                     TKTEKAQDSLNDNTTKTMTSDKPEDNQPK"
     gene            complement(24390..25370)
                     /gene="czrB"
                     /locus_tag="POB55_000446"
     CDS             complement(24390..25370)
                     /gene="czrB"
                     /locus_tag="POB55_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc efflux transporter CzrB"
                     /protein_id="HDI6499441.1"
                     /translation="MSHSHHHHDHMHSHVTTNNKKVLFISFLIIGLYMFIEIIGGLLA
                     NSLALLSDGIHMFSDTFSLGVALVAFIYAEKNTTTTKTFGYKRFEVLAALFNGVTLFV
                     ISILIVFEAIKRFFVPSEVQSKEMLIISIIGLIVNIVVAFFMFKGGDTSHNLNMRGAF
                     LHVIGDLLGSVGAITAAILIWAFGWTIADPIASILVSVIILKSAWGITKSSINILMEG
                     TPSDVDIDEVITTIKKDSRIQSVHDCHVWTISNDMNALSCHVVVDHTLTMKECELLLE
                     NIEHDLLHLNIHHMTIQLETPNHKHDESIICSGTHSHSHNHHAHHHAHAH"
     gene            complement(25372..25692)
                     /gene="czrA"
                     /locus_tag="POB55_000447"
     CDS             complement(25372..25692)
                     /gene="czrA"
                     /locus_tag="POB55_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)-responsive metalloregulatory
                     transcriptional repressor CzrA"
                     /protein_id="HDI6499442.1"
                     /translation="MSEQYSEINTDTLERVTEIFKALGDYNRIRIMELLSVSEASVGH
                     ISHQLNLSQSNVSHQLKLLKSVHLVKAKRQGQSMIYSLDDIHVATMLKQAIHHANHPK
                     ESGL"
     gene            25844..26509
                     /locus_tag="POB55_000448"
     CDS             25844..26509
                     /locus_tag="POB55_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDI6499443.1"
                     /translation="MNILVIGANGGVGSLLVQQLAKENVPFTAGVRQSDQLNALKSQG
                     MKAILVDVENDSIETLTETFKPFDKVIFSVGSGGNTGADKTIIVDLDGAVKSMIASKE
                     ANIKHYVMVSTYDSRRQAFDDSGDLKPYTIAKHYADDYLRRSGLNYTILHPGALTNAA
                     GSGKIEAAQYFDGKGEIPREDVATVLKEIVTSNHFNHQEFQIISGEQDIKDALTQFEI
                     EKD"
     gene            26945..27883
                     /locus_tag="POB55_000449"
     CDS             26945..27883
                     /locus_tag="POB55_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001620169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HDI6499444.1"
                     /translation="MPLFLQPILKTKLWGGQRLSEFGYQLDNDTTGECWCVSAHPNGT
                     SEIINGPYQGQTLDRIWSEHRELFGDFPSKDFPLLTKIVDARESLSIHVHPDNSYAYE
                     HENGQYGKSECWYIIDAEEDAEIVIGTLAESREEVANHVQHGTIESILRYIKVKPGEF
                     YFIPAGTVHTISSGILAYETMQSSDITYRLYDFNRQDNQYNDRPLNIEKALDVIQYNA
                     PLPNILPESEIIENHKCTHIVSNDFFTLVKWEISGTLNYMKPREFCLVTVLEGEGQMI
                     VDGEIFKLTTGTNFILTSEDLDSVFEGDFTLMISYV"
     gene            27914..28144
                     /locus_tag="POB55_000450"
     CDS             27914..28144
                     /locus_tag="POB55_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499445.1"
                     /translation="MKQFLYIALVCGVIAGLGAFLHIPQYPSMTIPRIVAILGIISAM
                     LTFKDKQISASLKFSALLINVLPLCGTFVASN"
     gene            28816..30189
                     /locus_tag="POB55_000451"
     CDS             28816..30189
                     /locus_tag="POB55_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HDI6499446.1"
                     /translation="MTAETNYFWLNCGYNRWNHNEPLVGQTALFESGAHFNPSQGFRA
                     FKKAKVGDQVIFYQVQTDTGLLGCGEIISVETGAQNKIRVQFRFNEQLKPLTADYLKR
                     SEALEFRMSNMKETLFNQITAEEFDLISGLGKGEIKIPRYFFLAETEEFEPGNQYTIY
                     THTYNGIKRNGYHFYTQLEEGDNIIFYNRTKNQSVVGIGEVSKHIHEKPPIPGRTNST
                     VIEVSYEKDITPITLSTLNKHPKLKNLYFLQENAKQAIASMSQAQYDAIIEMSDNNGL
                     KSPFEMVQKPDMLESEKEEALKPFILLVVDRKEEGLKAANDLLQKANANPVITTGHPD
                     FSEDMLYGKYLPNETGALYYREGFITQLMPKKDKSYLVIDNFNRIDTDIFQTYINVLE
                     GYEVTLPRYNKDGNMIKWSRQKDSFYYFNPNWHIVGITYDSLEEIKEKYSEQFLKYTR
                     IVKVKHD"
BASE COUNT        11141 a         4676 c         5395 g         9083 t
ORIGIN      
        1 taaatatatg ttcgtatgac agtgatgtaa atgattggta taatgggtat tatggaaaaa
       61 tattacccgg aggagatgtt atggattttt ccaacttttt tcaaaacctc agtacgttaa
      121 aaattgtaac gagtatcctt gatttactga tagtttggta tgtactttat cttctcatca
      181 cggtctttaa gggaactaaa gcgatacaat tacttaaagg gatattagta attgttattg
      241 gtcagcagat aagtatgata ttgaacttga ctgcaacatc taaattattc gatatcgtta
      301 ttcaatgggg ggtattagct ttaatagtaa tattccaacc agaaattaga cgtgcgttag
      361 aacaacttgg tagaggtagc tttttaaaac gctatacttc taatacgtat agtaaagatg
      421 aagagaaatt gattcaatcg gtttcaaagg ctgtgcaata tatggctaaa agacgtatag
      481 gtgcattaat tgtctttgaa aaagaaacag gtcttcaaga ttatattgaa acaggtattg
      541 caatggattc aaatatttcg caagaacttt taattaatgt ctttatacct aacacacctt
      601 tacatgatgg tgcaatgatt attcaaggca cgaagattgc agcagcagca agttatttgc
      661 cattgtctga tagtcctaag atatctaaaa gtttgggtac aagacataga gctgcggttg
      721 gtatttcaga agtatctgat gcatttaccg ttattgtatc tgaagaaact ggtgatattt
      781 cggtaacatt tgatggaaaa ttacgacgag acatttcaaa cgaaattttt gaagagttgc
      841 ttgctgaaca ttggtttggc acacgctttc aaaagaaagg tgtgaaataa tatgctagaa
      901 agtaaatggg gcttgagatt tattgccttt cttttggcat tgtttttctt tttatctgtt
      961 aacaatgttt ttggaaatat ctttaacact ggtaatcttg gtcaaaagtc tagtaaaacg
     1021 attcaagatg tacccgttga aattctttat aacactaaag atttgcattt aacaaaagcg
     1081 cctgaaacag ttaatgtgac tatttcagga ccacaatcaa agataataaa aattgaaaat
     1141 ccagaagatt taagagtagt gattgattta tcaaatgcta aagctggaaa atatcaagag
     1201 aagtatcaag ttaaagggtt agctgatgac attcattatt ctgtaaaacc taaattagca
     1261 aatattacgc ttgaaaacaa agtaactaaa aagatgacag ttcaacctga tgtaagtcag
     1321 agtgatattg atccacttta taaaattaca aagcaagaag tttcaccaca aacagttaaa
     1381 gtaacaggtg gagaagaaca attgaatgat atcgcttatt taaaagccac ttttaaaact
     1441 aataaaaaga ttaatggtga cacaaaagat gtcgcagaag taacggcttt tgataaaaaa
     1501 ctgaataaat taaatgtatc gattcaacct aatgaagtga atttacaagt taaagtagag
     1561 ccttttagca aaaaggttaa agtaaatgtt aaacagaaag gtagtttagc agatgataaa
     1621 gagttaagtt cgattgattt agaagataaa gaaattgaaa tcttcggtag tcgagatgac
     1681 ttacaaaata taagcgaagt tgatgcagaa gtagatttag atggtatttc agaatcaact
     1741 gaaaagactg taaaaatcaa tttaccagaa catgtcacta aagcacaacc aagtgaaacg
     1801 aaggcttata taaatgtaaa ataaatagct aaattaaagg agagtaaaca atgggaaaat
     1861 attttggtac agacggagta agaggtgtcg caaaccaaga actaacacct gaattggcat
     1921 ttaaattagg aagatacggt ggctatgttc tagcacataa taaaggtgaa aaacacccac
     1981 gtgtacttgt aggtcgcgat actagagttt caggtgaaat gttagaatca gcattaatag
     2041 ctggtttgat ttcaattggt gcagaagtga tgcgattagg tattatttca acaccaggtg
     2101 ttgcatattt aacacgcgat atgggtgcag agttaggtgt aatgatttca gcctctcata
     2161 atccagttgc agataatggt attaaattct ttggatcaga tggttttaaa ctatcagatg
     2221 aacaagaaaa tgaaattgaa gcattattgg atcaagaaaa cccagaatta ccaagaccag
     2281 ttggtaatga tattgtacat tattcagatt actttgaagg ggcacaaaaa tatttgagtt
     2341 atttaaaatc aacagtagat gttaactttg aaggtttgaa aattgtttta gatggtgcaa
     2401 atggttcaac atcatcatta gcgccattct tatttggtga tttagaagca gatactgaaa
     2461 caattggatg tagtcctgat ggatataata tcaatgagaa atgtggctct acacatcctg
     2521 aaaaattagc agaaaaagtt gttgaaactg aaagtgattt tgggttagca tttgacggcg
     2581 atggagacag aatcatagca gtagatgaga atggtcaaat cgttgacggt gaccaaatta
     2641 tgtttattat tggtcaagaa atgcataaaa atcaagaatt gaataatgac atgattgttt
     2701 ctactgttat gagtaattta ggtttttaca aagcgcttga acaagaagga attaaatcta
     2761 ataaaactaa agttggcgac agatatgtag tagaagaaat gcgtcgcggt aattataact
     2821 taggtggaga acaatctggg catatcgtta tgatggatta caatacaact ggtgatggtt
     2881 tattaactgg tattcaatta gcttctgtaa taaaaatgac tggtaaatca ctaagtgaat
     2941 tagctggaca aatgaaaaaa tatccacaat cattaattaa cgtacgcgta acagataaat
     3001 atcgtgttga agaaaatgtt gacgttaaag aagttatgac taaagtagaa gtagaaatga
     3061 atggagaagg tcgaatttta gtaagacctt ctggaacaga accattagtt cgtgtcatgg
     3121 ttgaagcagc aactgatgaa gatgctgaaa gatttgcaca acaaatagct gatgtggttc
     3181 aagataaaat gggattagat aaataaatac cataaatact gtattacaaa taagccgatg
     3241 cgtatgcatc ggttttttgt gtttctagaa ataatttata gtacaaacgt aaaatgatat
     3301 aaacaaaata aaaacaaagt aataaatatg taatataaaa tacactggta ctcaatatat
     3361 aatgatgata aaattaattt taattagata gagttgcttt gtgtttttaa cgcagatgct
     3421 actacttatc ttaacagttg attaagtgaa atcatttaac agcgagaata atcaaccagg
     3481 aggatgactt aatgaattta ttcagacaac aaaaatttag tatcagaaaa tttaatgtcg
     3541 gtattttttc agctttaatt gccactgtta catttatatc tattaacccg acaacagcgt
     3601 ctgcagcaga gcaaaatcag cctgcacaaa atcaaccagc acaaccagct gatgccaatg
     3661 cacagcctaa cgcaaatgct ggtgctcaag ctaatcctgc agcacaacca gctgcacctg
     3721 cgaaccaagg acaaccagca gcacaaccag caaaccaagg tggacaggct aatccagcag
     3781 gaggcgcagc acaaccagta ggaggcgcag cacaaccggc agcaggtaca gcacaaccag
     3841 ctggacaagg taatcaagct gatccaaata acgctgcaca agcacaacct ggaaatcaag
     3901 cagcaccggc aaaccaagca ggacaaggaa ataaccaagc aacaccggca aatcaaacac
     3961 agccagcgaa tgctccagca gcgcaaccag cagcacctgt agcagcaaac gcacaaactc
     4021 aagatccaaa tgctagcaat actggtgaag gcagtattaa tacaacatta acgtttgatg
     4081 atcctgccat atcaacagat gagaatagac aggatccaac tgtaactgtt acagataaag
     4141 taaatggtta ttcattaatt aacaacggta agattggttt cgttaactca gaattaagac
     4201 gaagcgatat gtttgataag aataaccctc agaactatca agctagggga aatgtagctg
     4261 cattaggtcg tgtgaatgca aatgattcta cagatcatgg taactttaac ggtatttcaa
     4321 aaactgttaa tgtaaaacca gattcagaat taattatcaa ctttactact atgcaaacga
     4381 atagtaagca aggtgcaaca aatttagtta ttaaagatgc taagaaaaat actgaattag
     4441 caactgtaaa tgtagctaag actggtactg cacacttatt taaagtacca acagatgctg
     4501 atcgtttaga tttacaattt attcctgaca atacagcagt tgctgatgct tcaagaatta
     4561 caacaaataa agatggttat aaatactatt cattcattga taatgtaggt ctattctcag
     4621 gatcacattt atatgtcaaa aatagagatt tagcaccgaa agcaacaaat aataaagaat
     4681 ttacaattaa tactgaaatc ggtaacaatg gtaattttgg tgcttcattg aaagcagatc
     4741 aatttaaata tgaagtaaca ttaccacaag gtgtaactta cattaatgat tcattaacta
     4801 caacattccc taatggtaat gaagacagta cagtattgaa aaatatgact gttaattatg
     4861 atcaaactgc aaataaagtt acatttacaa gccaaggtgt gacaacggca cgtggtacac
     4921 acactaaaga agttttattc ccagataaat ctttaaaatt atcatataaa gttaatgttg
     4981 cgaatatcga tacacctaaa aatattgatt ttaatgaaaa attaacatat cgtactgctt
     5041 cagatgttgt aattaataat gcgcaaccag aagttacact aactgcagat ccattttcag
     5101 tagcggttga aatgaacaaa gatgcgttgc aacaacaagt aaactcacaa gttgataata
     5161 gtcattacac aacagcatca attgcagaat acaataaact taaacaacaa gcagatacta
     5221 ttttaaatga agatgcgaat catgttgaaa ctgcaaatcg tgcatctcaa gcggatattg
     5281 atggtttagt aactaaatta caagctgcat taattgataa tcaagcagca attgctgaat
     5341 tagatactaa agctcaagaa aaggttacag cagcacaaca aagtaaaaaa gttacgcaag
     5401 atgaagttgc agcgcttgtg actaaaatta acaatgataa aaataatgca atcgcagaaa
     5461 ttaataaaca aacaacatca caaggtgtca caactgaaaa agataatggt atcgcagtgt
     5521 tagaacaaga tgtgattaca ccaacagtta aacctcaagc gaaacaagat attatccaag
     5581 cagttacaac tcgtaaacaa caaattaaaa agtcaaatgc atcattacaa gatgaaaaag
     5641 atgtagcaaa tgataaaatt ggtaaaattg aaacaaaggc aattaaagat attgatgcag
     5701 caacaacaaa tgcacaagta gaagccatta aaacaaaagc aatcaatgat attaatcaaa
     5761 ctacacctgc tacaacagct aaagcagcag ctcttgaaga atttgacgaa gttgttcaag
     5821 cacaaattga tcaagcacct ttaaatcctg atacaacaaa tgaagaagta gcggaagcta
     5881 ttgaacgtat taatgcagct aaagtttctg gtgttaaagc aattgaagcg acaacgactg
     5941 cacaagattt agaaagagtt aaaaacgaag aaatctcaaa aattgaaaat attactgact
     6001 ctacgcaaac aaaaatggat gcctataatg aagttaaaca agctgcaaca gctagaaaag
     6061 ctcaaaatgc tacagtttca aatgcaacaa atgaagaagt agcagaagct gatgcagcag
     6121 tagaagcagc tcaaaagcaa ggtttacatg acatccaagt tgttaaatca aaacaggaag
     6181 ttgctgatac aaaatcaaaa gtattagata aaatcaatgc aattcaaaca caagcaaaag
     6241 ttaaacctgc agctgataca gaagtagaaa acgcatataa tacacgtaaa caagaaattc
     6301 aaaatagcaa tgcttcaact acagaagaaa aacaagctgc atatacagaa ttagatacta
     6361 aaaagcaaga agcaagaaca aatcttgatg ctgcaaatac aaacagtgct gtaacaacag
     6421 ctaaagacaa tggtattgct gcaattaatc aagttcaagc tgccacaact aagaaatcgg
     6481 atgctaaagc agaaattgct caaaaagcaa gtgaacgtaa aacagcaatc gaagcaatga
     6541 atgattcgac tactgaagaa caacaagcag cgaaagaaaa agttgatcaa gctgttctgt
     6601 ctgcaaacgc tgatatagat aatgctgcag caaatacaga tgtagataat gcaaaaacta
     6661 caaatgaagc tacaatcgca gccattacac ctgatgcaaa tgttaaacct caagcgaaac
     6721 aagcaattgc agataaagta caagctcaag aaaaagcaat tgatgctaat aacggttcaa
     6781 caacagaaga aaaagcagct gcgaaacaac aagtccaaac tgaaaaaacg actgctgatg
     6841 cagcaataga tgcagcacat acaaatgcgg aagttgaagc ggctaaaaat gcagaaattg
     6901 ctaaaattga agcgattcag ccagcaacaa caactaaaga taatgcgaaa gaagcaattg
     6961 ctacgaaagc gaatgaacgt aaaacagcaa tcgctcaaac gcaagacatt actgctgaag
     7021 aaattgcagc ggctaatgcg gacgtagata atgctgtgac acaagcaaat agcaacattg
     7081 aagctgctaa tagtcaaaat gatgtagacc aagcgaaaac gacaggtgaa actagtattg
     7141 atcaagtaac accaacagtt aataaaaaag caactgcacg taatgaaatc acagcaattt
     7201 taaataacaa attgcaagag attcaagcta cgccagatgc aacagatgaa gaaaaacaag
     7261 cagctgatgc agaagcaaat actgaaaatg gtaaagcaat tcaagccatt gcagcagcaa
     7321 ctactaacgc acaagttgat gaagctaaaa caaatgcaga agcagcgatt aatgcggtaa
     7381 caccaaaagt tgtgaagaaa caaacggcta aagatgaaat tgatcaatta caagcaacgc
     7441 aaacaaatgt tatcaataat gatcagaacg ctacaactga agaaaaagaa gcagctattc
     7501 aacaattagc aacagcagtt acagacgcga aaaataatat tacagctgca actgatgata
     7561 atggtgtaga tcaggcgaaa gacgctggaa agaattcaat tcaaagcacg caaccagcaa
     7621 catcggttaa atcaaatgct aaaaatgatg ttgatcaagc tgtgacaact caaaatcaag
     7681 caattgataa tacaactggt gctacaactg aagagaaaaa tgcagcaaaa gatttagttt
     7741 taaaagctaa agaaaaagcg tatcaagata tcttaaatgc acaaacaact aatgatgtta
     7801 cgcaaattaa agatcaagca gttgctgata ttcaaggtat tactgcagat acaacaatta
     7861 aagatgttgc gaaagatgaa ttagcaacaa aagcaaacga acaaaaagcg cttattgcac
     7921 aaactgcaga tgcgactact gaagaaaaag aacaagcaaa tcaacaagta gacgcacaat
     7981 taacacaagg taatcaaaat attgaaaatg cacagtcaat tgatgacgta aacactgcaa
     8041 aagataatgc aattcaagcc atcgacccaa ttcaagcatc aacagatgtt aaaacgaatg
     8101 caagagcgga attgctaact gaaatgcaaa ataaaataac tgaaatactt aataataatg
     8161 agactactaa tgaagaaaaa ggtaacgata ttggaccagt tagagcagca tatgaagaag
     8221 gtttaaataa tattaatgca gcaactacta caggtgatgt aactactgct aaagatacag
     8281 cagtacaaaa agttcaacaa cttcatgcaa atcctgttaa gaaaccagca ggtaaaaaag
     8341 aattagatca agctgcagct gataagaaaa cacaaataga acaaacacca aatgcatcac
     8401 aacaagaaat taatgatgca aaacaagaag ttgatactga attaaatcaa gcgaaaacaa
     8461 atgtcgatca atcatcaaca aatgaatatg ttgataatgc agttaaagaa ggaaaagcta
     8521 aaattaatgc agttaaaaca tttagtgagt acaaaaaaga tgctttagct aaaattgaag
     8581 atgcatataa tgctaaagta aacgaagcgg ataactctaa cgcatcgact tcaagtgaaa
     8641 ttgctgaagc gaaacaaaaa cttgctgaat taaaacaaac tgcggatcaa aatgttaatc
     8701 aagctacttc taaagatgac attgaagttc aaattcataa tgacttagat aatattaacg
     8761 attacacaat tccaacaggt aaaaaagaat cagctacaac agatttatat gcttatgcag
     8821 atcagaagaa aaataatatt tcagctgaca ctaatgcaac acaagatgaa aagcaataag
     8881 caattaagca agttgaccaa aatgttcaaa ctgcattaga aagcattaat aatggtgtgg
     8941 ataatggtga cgttgatgat gcattaacac aaggtaaagc agcaattgat gctattcaag
     9001 tagatgctac tgttaaacct aaagcgaacc aagctattga agttaaagca gaagatacga
     9061 aagaatctat tgatcaaagt gaccagttaa ctgctgaaga aaaaactgaa gcattagcaa
     9121 tgattaaaca aattacagat caagctaaac aaggtattac tgatgcaaca acaactgctg
     9181 aagttgaaaa agcgaaagct caaggacttg aagcatttga taacattcaa atcgactcaa
     9241 cagaaaaaca aaaagctatc gaagaattag aaactgcact agaccagatt gaagcaggtg
     9301 taaatgtcga cgctgatgct acaactgaag aaaaagaagc gtttacgaat gctttagaag
     9361 acattttatc aaaagcaact gaagatattt ctgatcaaac tacaaatgca gaaatcgcta
     9421 ctgtcaaaaa tagtgcgctt gaacaactta aagcacaacg tattaatcca gtagttaaga
     9481 aaaatgcttt ggaagcaatt agagaagtgg ttaacaagca aatagaaata attaaaaatg
     9541 cagatgcaga tgcatcggcg aaagaaattg cacgtacaga tttaggtaga tattttgaca
     9601 gatttgctga taaattagat aaaacacaaa caaatacaga ggttgctgaa ttacaaaatg
     9661 taacgatacc tgcaattgaa gcgattgtgc ctcagaatga tccagatgct aatgatacta
     9721 ataatggtac agataataat gatgcaacag caaattcaaa tgctaatgca acaccagaaa
     9781 acactggaca acctaacgta tctgagacga cagataatgg caaagcagac gcttctccga
     9841 caacaccaaa taatagtgat gcggcaacag gtgaaacaac agtaacatca gcaactgatg
     9901 atgcaaagga taaaccgcaa gctaataaca attctagtgc agatgcatca acaaacagtc
     9961 cgacaatgga taacgatgta actagtaaac cagaagtaga atcaactaat aacggcacaa
    10021 ctgataaacc tgtaacagaa acagataatg caacgccagc agaaagcaca acaaataaca
    10081 atagtacaac tacagcaaca aacgagaatg caccaacagg atcaacaacg acggctccaa
    10141 caactgcgag cacagaagca gcatcatctg ctgatagtaa agataatgca tctgtaaatg
    10201 attcaaaaca aaatgctgaa gtaaataata gtgctgaatc tcaatcaact aatggcaagg
    10261 ttgcacaacc aaaatctgaa aataaagcta aggcagaaaa agatggtaga gattcaacaa
    10321 atcaaagtat ggttgaatca acaactgaaa cattgccttc agcagacata acagaaccaa
    10381 atgtaccttc taatacatca aaagataaag aggaaagtac gtcaaatcaa actgatgcag
    10441 gacaacttaa gtctgaaact aacgttgcat ctaacgaagc agataaatcg ccaagcaaag
    10501 ctgatactga agtttcgaat aaaccatcaa catctgcatc ttctgaagca aaagaaaaaa
    10561 tgacttcaac taatgttagc caaaaagatg atacggcaac agcagatact aatgatatgc
    10621 aaacatcagt tggctcagtt gcaaacaata aagctaaaga tatgcaaact aatgatacgc
    10681 aaacatcagt tggcccagtt gcaaacaata aagctacgca aaacgacggt gccaatgcat
    10741 ctccagcaac agtttctaat ggtagtcata gtatgcatca agatatgctt aatgtaacta
    10801 agactgaaga aaacaaagca aatgcaaaat cagaccaaca aggaaaagtt aataagccta
    10861 aacaacaagc taaaacttta ccagatactg gtatgtcaca taatgacgat ttaccatatg
    10921 ctgaattagc tctaggtgca ggtatggcat tcttaattag aagattcact aagaaagacc
    10981 aacaaactga agaataaaat ttattcaaga atgtgaacat ctagttattt catagggaaa
    11041 tattccttac taagtaacca agtctaacag ttaatatgaa tttgttttag taatgaccaa
    11101 tagccattga ataggtggct gtggctcgaa aataaaacaa attattaacg caaaaatact
    11161 cctttcatac ataacacaag ttaagtgtct atgttgaagg gagtattttt ttatgttcag
    11221 aaatttaata ttgttaattc aacggaacta ctacattatt actgattgtt aacgaataac
    11281 gttggaaact tcttgtttca aacaaagctc aataaattat gaaagtgaat tgtacgcttc
    11341 aattatttca tcggctaagc catcgtcaac tttagcatat tcgcttaaaa acgcttttaa
    11401 accgtgttct ttaatgtaat tatttttctc aacagtttct tggtctgctg tatcatcata
    11461 cttcaataac aatgcagctg tatttaataa accagtgcgt tctaaatctt tattataaag
    11521 atatgttaat ggttttataa ttctatcttt agggccaatt ttacgtaacg tacctcgtcc
    11581 gacacgtgtc acttcatcag ataaataaga attgttgaaa cggtctatta ttttttcaac
    11641 ataaccagct tgttccgctt cagtaaaatc aaattcatta gtaatatatt gacttgtttc
    11701 agctaaaaca cggcgtaagc cagcttcaat tgaactatct tttacagcgt ctaatacggt
    11761 agctttacct gcaaatttac cagcatacgc taaatatgca tgtcctgtat tcacagttaa
    11821 taatttacgc tcaatatatg gtgttaaatc atcaacatat ttaatatgat ttagttctgg
    11881 gccataccat gcatcttttt caacaaccca ttcgtaaaat ggctcaacca taacgtctaa
    11941 tatattttca ttcttttgta atggtacaat tctatcaact gctgagttag caaaatgaat
    12001 attgttacca agagggccag taatatcaag tactgctttt ttcaatgtat cagttgccat
    12061 aatagcattc tcacaagcaa caatattaac atggtttgtt ttttctttta agaaaggcgc
    12121 aaaagattta gcaataatag gtagtatgtt aacaccaaca gctgttgtaa taatatcagc
    12181 ttctagaatt gcttgtttca acgcttcaga aggttgcatt gaattaattg catcaacatt
    12241 attcacgcgc gtcgttgttt tagactcatc agctaaaata acatcgtatt gatgatcatg
    12301 agctaaagca ttaatgattt cttcattgac gtctgcaaat gttactttaa cattgttgtc
    12361 tgcaagaata taaccaatga aaccacgacc tatgttacca gcaccaaagt gaactgcttt
    12421 cattatgcat ctgcctcctc gaatacttgt ttaatttctt ctgcagattt tgcttgaacg
    12481 atacgatcca cattttcttc ttcgctaaat gtaattgcaa ttttagataa caagtctaaa
    12541 tgttcgccat ctttaccagc gattcccacg acaactttaa ctacttcgcc atcccagtca
    12601 acgccttcag ggatttgtaa taatgttaaa cctgagtgaa tcacatttgt tttagcttca
    12661 tctgtgccat gaggaattgc taagccattt cccataaatg ttgatacgac ttgttcacga
    12721 tctttcattg cttgaatata agcatctgtt acagcaccac tatcaactaa ggctttacct
    12781 gctttttcaa ttgcttcatt ttggctgtta acatttacat ttaaaaagat attgtcatta
    12841 ctaaataatt cgctcatttt gtacactcct aaataaattg ttttttaatt cgttctaaaa
    12901 atttgtttct taataatgtt tcaagttcct gtggattttc cataaaagta tcaatagatt
    12961 ctaatttcaa acttaaaaat tcggacaagt cacttaccag tgatgccata aaatcattgt
    13021 caggaataaa catggaaatc atatatttaa ttgtttgatt gtcattttga atactaggca
    13081 atattaacgg ttcttctaaa actgtgatta gaaccatagg gtgtttaatc atattgtctc
    13141 ttaggtgtgg tatcgcaaca ggatatggac ttaatatcca tcctggattg tcaatcaatc
    13201 gttgctctag tagttcagcg aatgacgttg gatcgtttat tgcatcgcat tgatatagat
    13261 aatctgcgat atatgtttgc cactgtttaa cttcggtact aactattttc actgaatcaa
    13321 gcaaagttaa acccgaacgc atatgatgca ttaattgttc tgatttttcg tcacttaaat
    13381 gtttttcgaa aactgaagtt aagtatgaag tttgttcatt ttcaaaagat gtattgccat
    13441 caatatcttt cgtttcaaca tgaacatcat ccttatcaat catgtttgat gatttatcat
    13501 gcgtctcttg gaacgtagac tttgtattta aaaactgtgc gacataactg atatcactat
    13561 ctggtaataa tgggtttacc gttaaatagg gggagtcgat gtctaaattc acagtagaaa
    13621 taatgccatc atattgactt aaatctagtg acttcaaatc gctgactgat gcttgtgtaa
    13681 tacgctcaat ctcactaaaa acttgctcta gacgagttga taatagacga ctagttccca
    13741 taccactgct gcaaacgact aatatgttta aaaatcgatt accttggttt ttaatcgagc
    13801 caccaaagtg taaaactata aaagcaattt cactctctgg aaaaatcaaa tcaggccaac
    13861 aatcatttaa agctaatctt acattttcaa atagtcttgg atatttgaac ttaatcatgt
    13921 ctgttaacgg attataggtc tcgatattag cttgcaaacg attgattgca ggattaaggt
    13981 gtagcgtcag tccatctact aaagtatcca aatctgcaaa tgtcataccg gaaatcattt
    14041 ctactttgtt aacaaacgct ttaattttgt tttcttcaca acgattattt aatgatgtat
    14101 cattaagatt ttttcgttta gctccacgta aatggatagt aatgaaagca atttctgcct
    14161 gattaaacgt gacgtcatat atttgaccaa gtttatcagc aagttcgctt gctactttgt
    14221 gttcaaatgt gttttgtaca gaatcataaa tatcatcgtt taatgcgaca tactcaccat
    14281 tttttatacg tgaaatggat agcacgatat ggacagttaa agttaaataa cttgattcgg
    14341 taagttggta gggtaaggca cttaggtagt ccattaatag tctttcaaca ttaaaaatgc
    14401 gctctaagtc aacaaagtct ttgtggattt gatttaatga atgaaagaca aaatgatttt
    14461 caattactga ataaacgcta gtactattta agttattcac cattaattga cttaaaaatt
    14521 cacgtttctt tgattcagta cctaccaagt aaatgccttc gccacgcttt cgagataaag
    14581 atagttggta cttatttaaa tcaagctcta aatcatctaa catctttgct aaagtctgga
    14641 cagaaacgcc aatttcttgc gctaaactat attgtttaac tggctccttg gcttgtatca
    14701 aagcgtatat aataattact ttctgctctt caacagataa gtcaatggtt tgatgttgtg
    14761 caatcgattg cttcaaatcg tttaaatcag aatctgtgcc agcaatgcgt agcccttttt
    14821 tgtttgcgcg ttctaaagtt aatgaaaatg aagttaaata tgcttcaaca ccttttaatt
    14881 cacggtgaat agtacgagag gacaccgcaa gttgctgagc aatgtcgtgt atagtgatat
    14941 attgaccgtg gtacttaatc aatagggcta tcatttcttt ttcacgtgta ctcaataaca
    15001 tggcggtatc ctccattttt taatttattt aattattatg cttgatcatc tttttttaga
    15061 ttatttaaaa gttcttcata tcttggtgaa ttaaggaaat tatccactga aatatggatg
    15121 gcatttggtg tttgtttaat agcacgatca gttagttttt tctgagtgat aactaattga
    15181 gcatcttttg gcaattgatt aatcgcagta tttgtaactg taatatcatt aatgcccgcc
    15241 tttttaaatt tattacgtaa catgcttgca cccattgcac tagaacccat acccgcatcg
    15301 caggcaaaaa taacatgatt tatattattg taattgtgtg catcaacatc ttcagtgttg
    15361 taattatcca atagcgcttc aggatcatca tctgatgaag atgtttcact aacattacca
    15421 ctagcatttt cttctgtatt aacattttta tcagaagata ctaacttaga agcaacgctt
    15481 gattttttcc ctttagtatt ttccatttga gctgtcgcag cttctaaatc ctgctttggt
    15541 tctctagtga acttcataat taaagcagct acaacgaatg atacaagtgc agcaaggaag
    15601 acaccgagta acatgtgcaa gaattcacct ctaggtgcat ttaaacagta aactataaat
    15661 gaacctggtg acgcgggact tttaaatcca aatcctgttg cttgataagt tgcaacacca
    15721 gtcattccac ctaaaataac agcgataaat aataaaggac gcattaatac atatgggaaa
    15781 taaatttcat gaataccacc taagaagtgg ataattccag caccatatga cgttgctttt
    15841 gcagtgcctt ttccaaaaat catataagca agtaagatac ctaaacctgg tccagggtta
    15901 gattcaattg tgtataaaat tgattgacca gcttttgcag cttgatctgc accaagcggt
    15961 gtgaatacac catggttaat cgcattgttt aaaaatacaa tttttgcagg ctctactaaa
    16021 atacttacaa gtggaagtag gtgtgcatgt actaatgctt caactgccac tgataaaata
    16081 tgcataataa atttcataag tggtgctaaa attttaaatc ctgcaatcgt catgataaat
    16141 cctaaaatac cagcagaaaa gttattaaat aacatttcaa aaccttgcgg cgttctaggt
    16201 tgaatcaatt ggtcggtctt cttcattaac caaccaacaa gtggacccat aatcattgca
    16261 ccaagtaaca ttggtgtatc aggtaatgca acgatgaccc ccatagttgc tgttgctgcg
    16321 atgataccac cacgtaaatc ataaattaaa cgaccaccac taaatgcgat caataatggg
    16381 attaaataag taatcattgg tcctgctaaa gtagctaaat ctttgttagg taaccatcca
    16441 ttatctataa aaatggccgc gataaaaccc caagcgatga aagcgccaat gtttggcatg
    16501 atcatactac ttaagaatga tccaaatgct tgaacacgac gaccaattcc ttttttctct
    16561 tcagtttgtg acatgtttac acctcttttt taatatatgt gactatatat agtgtataga
    16621 gaggttactt gttactcaat ataaacaaaa atcaactttg tcaaaataaa tgtgacaaaa
    16681 ttaaataaag tgtcatcaat gtgacagtat agatattttg aaaaagtaaa acaaaaaaat
    16741 tgttttagga tttttaaaat tttattgtga aaatatttgc aaaacaaaac aacaccgttg
    16801 tacaataatg attaatggaa agggggaaag ttcggcagta cagttaaagc gcctgtgcaa
    16861 ataaatattt gtatttgaag attaaaggtt aatatatgag tggcctttat agagtgcaat
    16921 atatgtattt gtagacgagg aggatagtga tcgaatagat cggcggatgc tatcccggat
    16981 gtggctcatt cgttagctta ttaagtaaaa cattagggtg acttaatgga caaagttaat
    17041 aagatcgcca gaaattgaat ataaaaaata ttaatatgga aagtacagtg tgagcaattt
    17101 gtatagttgt aaaaataact atgcttaatt tgttatggat gaatgcgatg atagcatgtt
    17161 cctatttata ttatgaaagc agattgtcaa tctaaattat cggcaataaa tcataattta
    17221 cgcgtactat tccaatatgg aggaaaatgt cgttatgtgt ggaattgttg gttatattgg
    17281 ctatgataat gccaaagaat tattattaaa aggtttagaa aaattagaat acagaggtta
    17341 tgactctgca ggtatcgcag tagtaaatga tgataataca actgtattta aagaaaaagg
    17401 tcgtattgca gaattacgta aagttgctga tagtagcgat tttgatggac ctgttggaat
    17461 cggtcacaca cgttgggcaa cacacggtgt accgaatcat gaaaactctc atccacatca
    17521 atcatcaaat ggccgtttta ctctagttca taacggtgtt attgaaaact atgaagagtt
    17581 aaaaggtgaa tacttacaag gtgtatcatt catttcagaa acagatacag aagttatcgt
    17641 tcaattagtt gaatactttt caaatcaagg actttcaact gaagaagcat ttacaaaagt
    17701 tgtgtcatta ttacatggtt catatgcatt aggtttatta gatgctgaag acaaagacac
    17761 aatctatgtt gctaaaaata aatcaccatt attattaggt gttggtgaag gtttcaatgt
    17821 tatcgcatca gacgcacttg caatgttaca agtgacaagc gaatataaag aaatccatga
    17881 ccatgaaatc gttattgtta aaaaagatga agttattatt aaagatgcag atggaaacgt
    17941 tgtagaacgt gattcatata ttgctgaaat tgatgcatca gatgctgaaa aaggtgttta
    18001 tgcacactac atgttaaaag aaattcatga acaaccagca gtaatgcgtc gtattattca
    18061 agaatatcaa gatgcagaag gtaacttgaa aattgatcaa gacatcatca atgatgttaa
    18121 agaagcagac cgcatttacg ttattgcagc aggtacaagc taccatgcag gtttagtagg
    18181 taaagaattt ttagaaaaat gggctggcgt accaactgaa gtacacgttg catcagagtt
    18241 tgtctacaac atgccattat tatctgaaaa accattgttc gtttatattt ctcaatcagg
    18301 tgaaactgca gatagccgcg ccgtattagt tgaaactaat aaattaggtc ataaatcatt
    18361 aacaatcact aatgttgcag gttcaacttt atcacgtgaa gcagaccaca cattgttatt
    18421 acacgcgggt cctgaaatcg cagttgcatc tacaaaagca tatactgcac aaattgcagt
    18481 attatcaatc ttgtctcaaa tcgttgcaaa agagcatggt cgtgaagcag atattgattt
    18541 attgagagaa ttagcaaaag taacaacagc aatagaagca attgttgacg atgcaccaat
    18601 tatggaacaa attgctacag atttcttaga aacaacacgc aatgcattct ttatcggacg
    18661 tactattgac tataacgtaa gtttagaagg tgcgttaaaa cttaaagaaa tttcttacat
    18721 tcaagcagaa ggttttgctg gtggagaact taaacatggt acaattgcct taatcgaaga
    18781 aggtacacca gttgtaggtt tagcaacaca agagaaagtt aatttatcaa ttcgtggtaa
    18841 cgttaaagag gtagtagcac gtggtgcaca tccatgtatt atttctatgg agggtcttga
    18901 aaaagaaggc gacacttatg tcattcctca tgtacatgaa ttgttaacgc cattagtatc
    18961 agtggttgca ttacaattaa tttcatacta tgcagcatta cacagagatt tagatgttga
    19021 taaaccacgt aaccttgcta aatcagttac tgtggaataa ttcacttttt tagaatcaat
    19081 catgtattaa aattaaagta tatggcaccc ttttagatta atcgactaga agggtgcttt
    19141 tttaggtcga cttagctttt acttcatctt aatttggcag aaatgcgtaa aaatgaagtg
    19201 ttttatttat ttaaatagtc tgacaattaa gggtgttatg ttaatatgat tttatgagaa
    19261 gtatggagta gcaataaagg ggtgacctcg catgttaatt caattagatc aaattgggcg
    19321 aatgaagcaa ggaaaaacaa ttttaaaaaa gatttcttgg caaattgcta aaggtgataa
    19381 atggatatta tatgggttga atggtgctgg caagacaaca cttctaaata ttttaaatgc
    19441 gtatgagcct gcaacatctg gaactgttaa ccttttcggt aaaatgccag gcaaggtagg
    19501 gtattctgca gagactgtac gacaacatat aggttttgta tctcatagtt tactggaaaa
    19561 gtttcaagag ggtgaaagag taatcgatgt ggtgataagc ggtgctttta aatcaattgg
    19621 tgtttatcaa gatattgatg aagaagtacg taatgaagca catcaattac ttaaattagt
    19681 tggaatgtct gctaaagcgc aacaatatat tggttattta tctacaggtg aaaaacaacg
    19741 agtgatgatt gcgcgagctt taatggggca accccaggtt ttaattttag atgagccagc
    19801 agctggttta gactttattg cacgagaatc gttgttaagt atacttgact cattgtcaga
    19861 ttcatatcca atgcttgcga tgatttatgt tacgcacttt attgaagaaa ttactagtaa
    19921 ctttactaaa attttgctat taaaagatgg ggaaagtgta caacaaggat taatagatga
    19981 tattttgaca tctgaaaaca tgtcacgatt tttccagaaa aatgtagcag ttcaaagatg
    20041 gaataatcga ttttctatgg caatgttaga gtaaatattt tgcaaatact aactaataat
    20101 gacaaaattt aattaagata aaatggacag tggagggcaa tatggataac gtaaaagcaa
    20161 tatttttgga catggatgga acaattttac atgaaaataa tcaagcatca ccgtatacga
    20221 aagatgtcat taatcgattg agagagaaag gatataaagt atttttggca acaggacgtt
    20281 cgcattctga aatacatcaa cttgtacctc aagattttgc ggttaatggc atcattagtt
    20341 caaatggaac aattggagaa gtagatggag aaattatctt caagcatggt ttatcattgg
    20401 ctcaagtgca acaaattact aatttagcta agcgccaaca aatttattat gaggtatttc
    20461 cttttgaagg taatagagtt tctttaaaag aagatgaaac atggatgcga gatatgattc
    20521 gtagtcaaga tcctattaat ggcgtaagtc atagtgaatg gtcttcaaga caagatgcgc
    20581 ttgctggtaa gatagattgg gtaactaagt ttcctgaagg tgaatattca aaaatttatc
    20641 tattcagttc taatttagaa aaaataacag catttagaga tgaattaaag caaaatcatg
    20701 tgcaactaca gattagtgtt tcaaattcat caagatttaa tgcggaaaca atggcttatc
    20761 aaactgataa aggtacaggc attaaagaaa tgattgcaca ttttggtatt catcaagaag
    20821 aaacgttagt tattggagat agcgacaatg atagagcaat gtttgaattt ggtcattata
    20881 cagttgctat gaaaaatgca cgccctgaaa tccaagcatt aacttcagat gtaacggcat
    20941 acacgaatga agaggatggc gcagcaaaat atttagcaaa gcatttttta gctgattaat
    21001 aacatcggca gttatttatt atttaattta caatagttga agagtaattt acaaaggagc
    21061 agtaaagtta ttttctatta gaaaatgtct tactgctcct ttgtatgcta gtaatccaga
    21121 tactagtgtt atttcttctt tttgtctttc ttctccatat atggaatgaa gaaaatcaca
    21181 aatagggcac agagtatagc tacaattgaa ccgtatgtca tattagcatc accttttata
    21241 tggtttatgc atttaagtat actataacaa ctaccgttat tttaagtagg ggtgtatttg
    21301 cgttagagcc cctaacaact ccaaaatcgc atttgtgatt ttgggataat caaacatttc
    21361 attccataag taagatatgt ttcactttat atgagttttt tacattcaca ttctgtagtg
    21421 gttgtaagaa aattttttct ttctcttagg cagtaatagg caatttacct gtactagtat
    21481 gaacttagta gttaaatcgt tcattaagat taaaaaaact taggaatctc ttccaaatgc
    21541 aattcgtctt ggtaagcatt aatttcatct aatatttttg ttattgattc aactttatct
    21601 ttttctaggg aggccggaaa tacaaggttt tacttgaaat ttaaaaccat cagtgaatta
    21661 tttaaatcta cgatttattc gaccacttgt actttctttt cttggcttat acatgactat
    21721 tttcaagtta tccgttacaa gttcattata tacaccttct attagctcaa acgattgtgt
    21781 taatgaatta aataaggcat cacttgattt actcattgtt atgcatgatt ataaaacgag
    21841 aataataaag tagtagcaca cctatatata gtggaatcct ttgattatct ttcgttttaa
    21901 agttaatgct ttctttccag tcaaactgtg cttgatgatc aggttttgtc tcgaatcttg
    21961 ttgtatcttt aggcgacatc tattgataac atttcataat ataaggagta aattcatcat
    22021 gtttaagtat ataagctcta aatgtataat atgcacaggt taatccatgt ttttccttcg
    22081 aatattgcca aagtatacgc ttatagtaaa atttttgttc actagtgtct gaaagtaatt
    22141 tttcgaggat aggataatac ttatcaataa tagactgacg atttctcttt caaagttttc
    22201 atttttcaat gtctattttg aagggagcat atcagccgaa aaggttggtg ctcttcttta
    22261 ccagagaata aaaaatgaga aattaaattt ttatatgatg aacaaacgat aacaggggaa
    22321 gaaatagcta gttagacaag ggtatgtcac tcaactgttt atcgagttga ttaagaaatg
    22381 aaaaaactta tactatgaat tactgtttaa aagtgtgcat gttataatat ttattgagca
    22441 agttggatag atggtggcta atctcttaat aaaggggtga tgcctatggt tatagttgtt
    22501 actcctagaa aggactagca tgtctgattt tgaaatgctg atggttgtat taacaatcat
    22561 tggtttagta ttgattagta ctcaagacca taaaaaataa ccttctattc gctttgaccg
    22621 gcattttgaa ggctattttt aaataaaata taaggtcacc gtctttttaa cgggctcatt
    22681 agggtaacat gtttccgagt gttgcccttt ttgtgtttca agagttaaag attttcatct
    22741 tttgcttcta cttactacaa atatatttta acacattttt cttatgaatt gtagttctga
    22801 acataatcag aattaataaa accaactttc catacagcag aaaatacaat taaaagtata
    22861 gaatgtaatc acgaattata taaaaagtat tggagacctc ttcatatata gataattcac
    22921 ttagttattt tagaaagaag cccctaacaa ctaaagttga aaagtaggag cgcctgcgct
    22981 aagcgcatgc ataagagccc ctaacaacta aagttgtaag gggctctaaa atttattttg
    23041 gttgattgtc ttctggttta tctgaagtca ttgtttttgt tgtattatca tttaatgaat
    23101 cttgtgcttt ttctgttttt gtttcaacag atgttgtcac tttatcattt tctacttttg
    23161 tattttttgg ttcagtgaca acttttttat tcttgtcagc tgtttttact ttatctttag
    23221 taattttttg accacgtttt aagtaatatt gaacgatacc cattaaaatg attgcatgaa
    23281 taacaacgaa taaaataatt gtcattactg tataaactct tatgatattt tctgcaacac
    23341 tttgagagag tgattgcgta ttaaatgaaa tcaagtaacc aacaggtgtt ttaaataaaa
    23401 taataattaa gtttaataat agtattccaa tgaaaaattt gaaaaatgtt ttttgaccat
    23461 ttttcattgc tttaaatcca ttagctaagt gtgtttttac tttatcgtta gttgattcaa
    23521 cgaaactagt cataaagttg aaaacaggta ttaacaataa ccaagtaata attgctttaa
    23581 tcaataaaac aacaatgatt atgatgcttt gagttgtaat agcaatacca atcaggtgtg
    23641 tactatctgc atatgatgat tgtactgaat tcataatcat ttctgatact ggagaaaata
    23701 atttgtatag taatgatgtc agaagtgaaa gaacgataat cattgctata gatacaagac
    23761 ctatcagaac gctctttaaa taattacctt ttttaaatgc aataaataaa tcggtaaatt
    23821 ttactttttc atgactcatt gctcgtttca taacatttgt aattccgata aaaatttgaa
    23881 ctaaaacaaa cagtgaaact acagctgcta taagtaatac agcgataact tttaaatatg
    23941 catctactgg tggtttttgc ccaaattgtg aataaattgc aatagtttgt acatacttag
    24001 caagctggaa attagcaagt aaatatacaa ctgcaattac agcaaatgct ataagtgcat
    24061 atatcaatgt tattaataat tgtggtttag catttttaaa tgctgattta aagtaagtaa
    24121 acaaagtggt gcctcctttt cttcatataa atacaataac aaaaaacacg ttattttcat
    24181 attaatatct aataaaaatt aattaaagtg actaaagtta gagaattata atagtaaatt
    24241 gtcaaaaata tgactaatgt aagaaataat agttgaaaat ttgttaataa acattgcatc
    24301 atcaaagacc aaacaagtcg ccataaagac ataaaaaacc tactgccatt gaaaagttta
    24361 atcgatgcaa tggcagtagg ttaaaattat taatgtgcat gcgcgtgatg atgagcatga
    24421 tggttatgtg aatgactatg tgttcctgaa catataatcg attcatcatg tttgtgatta
    24481 ggcgtttcta attgaatagt catatggtga atatttaaat gtaataaatc atgctcaatg
    24541 ttttctaata ataattcaca ttctttcatt gtcaatgtat ggtctacaac aacatgacaa
    24601 ctcaatgcat tcatatcatt tgaaattgtc caaacatggc aatcatgcac actttgtatt
    24661 cgtgaatcct ttttaatagt agttataact tcatctatat caacatcact tggtgtgcct
    24721 tccattaaaa tgttaataga agattttgtg ataccccaag cactttttaa aataataacg
    24781 gaaactaaaa tacttgcgat aggatcggcg attgtccatc caaatgccca aattaaaata
    24841 gctgcagtaa tggcgccaac tgaacctaat aagtctccga taacatgtag aaaagcacca
    24901 cgcatattta aattgtgtga agtgtcgccg cctttaaaca taaagaatgc aacaacgata
    24961 ttgacaatta aaccaataat gctaatgatt aacatttctt ttgattgaac ttcagaagga
    25021 acaaagaaac gtttaatcgc ttcaaaaaca atcaaaatac ttattacaaa aagcgttaca
    25081 ccgttaaata acgctgcgag tacttcgaaa cgtttataac caaatgtttt tgtagttgtg
    25141 gtattctttt cagcataaat aaatgcgaca agtgcaacac ctaatgagaa tgtgtcgcta
    25201 aacatatgga taccgtcaga tagtaatgcc aagctgttag caaggagacc gccgatgatt
    25261 tcgataaaca tatatagacc gattattaaa aacgatataa acaatacttt cttattattt
    25321 gtagttacat gactatgcat atggtcatga tgatgatgtg aatgagacat attataaccc
    25381 actttcttta ggatgattcg cgtgatgtat ggcttgcttt aacatagttg ctacgtggat
    25441 gtcatctaat gaataaatca ttgattggcc ttgtcgtttt gctttcacaa gatgcacact
    25501 tttaagtaat tttaattggt gcgagacatt tgattgagat aaattcaatt gatgtgaaat
    25561 gtgaccaaca cttgcttcac tgactgataa caattccatg atacgtattc gattgtaatc
    25621 gcctaatgcc ttgaaaattt cagttacgcg ttctaatgta tctgtattta tttctgaata
    25681 ttgttctgac attatatgac aatcctttca tatgaatatt tgttcatata ttaatttaat
    25741 acgttcccag tagtatgtca attgattttt atagttgtaa caagttttac atatctcata
    25801 aagtggtatg gcatagagaa taataaatag gagagtgttg taaatgaata ttttggttat
    25861 aggtgctaat ggcggtgtag gttcgttact agtgcaacaa ttagcaaaag aaaatgtacc
    25921 atttactgct ggtgttaggc aatcagatca acttaatgcg ttaaaatcac aaggtatgaa
    25981 agcaattctt gttgatgttg aaaatgattc aatagagact ttaactgaga cgtttaaacc
    26041 attcgataaa gttatctttt cagtaggttc tggagggaac acgggtgcag ataaaacaat
    26101 tattgtcgat ttagatggtg ctgttaaatc aatgattgcg agtaaagagg ctaatattaa
    26161 acactatgtc atggtttcaa catatgattc gagacgtcag gcttttgatg atagcggtga
    26221 tttaaagcca tatacaatag ctaaacatta cgctgatgac tatttaagac gttcaggttt
    26281 gaattataca attttacatc caggggcact tacaaatgca gcaggatctg gaaagataga
    26341 agctgcacaa tattttgatg gtaaaggtga aattcctaga gaagatgttg cgacagtatt
    26401 aaaagaaatt gtcacatcca atcattttaa tcaccaagag tttcaaatta ttagtggtga
    26461 gcaggacatt aaagatgctt taactcaatt tgaaattgaa aaggattaat aaggatagta
    26521 aatatgattt gaagcgggtt acgttttcgt aactcgttat ttttttgaaa atgatgaatt
    26581 ataagtatga ataagtacgt attgtctatg cattttacaa agaatttgaa caatgtatgg
    26641 cacaactatt aaagttgttt attaattggt ataatgggcg agtaaattta aagggaaatg
    26701 tgtacaaatg taagagagag ataatctcta tgctattaat ttttaaaaat gttaaagtac
    26761 tgtaaattta agtgaagcgc tttaattggc agtactgcaa tgttagtaaa tatggtacaa
    26821 tcactaatag ttaatttgaa atgtggaagt ttataatacg atgtaaatga gaacaaaatc
    26881 aaaatataat ggaacaacaa catcagagtt ctattgctta cttaatcaca atggaggtgc
    26941 aacaatgcca ttatttttac aaccaatttt aaaaacaaaa ttatggggcg gtcaacgtct
    27001 aagtgagttt ggatatcaat tagacaatga tacaactggg gaatgttggt gtgtgtcagc
    27061 acatccaaat ggtacgagcg agattattaa tggaccatat caaggtcaaa cattagaccg
    27121 tatttggtca gaacatcgtg aattgtttgg tgatttccca agcaaagatt ttccgcttct
    27181 aactaaaata gtggatgcaa gagaatcact ttctattcat gtgcaccctg ataattctta
    27241 tgcttatgag catgaaaacg ggcaatatgg caaatctgaa tgttggtata ttatagatgc
    27301 agaagaagat gcagaaatag ttatagggac tttagcagag tctagagaag aagttgcgaa
    27361 tcatgttcaa cacggaacga tagagtcgat acttagatat attaaagtaa aacctggaga
    27421 attctatttt attccagcag gaacagtaca tactatttct tcaggaatat tagcatacga
    27481 aacgatgcaa tcgtcagaca ttacatatag actttatgat ttcaatcgtc aagataatca
    27541 atataatgat agaccgttaa atattgaaaa agctttagac gttattcagt acaatgcacc
    27601 attacctaat attttgcctg aaagtgaaat tattgaaaac cataagtgta cacacattgt
    27661 atcgaatgat ttctttacat tggttaaatg ggaaatttct ggcacgttaa attatatgaa
    27721 gcctagagag ttctgtttag ttacagtgtt ggaaggtgaa ggacaaatga ttgtcgatgg
    27781 tgaaattttc aaactgacta ctggtacaaa ctttattttg acttctgaag atttggatag
    27841 tgtctttgaa ggcgatttca cattgatgat tagctatgtg taatagtgta ataacaattt
    27901 aggagtgaat gatttgaagc aatttctata tattgcgtta gtatgtggtg tgatagcagg
    27961 tcttggtgct ttcttacata taccgcagta tccgagcatg acaattccac gtatagtagc
    28021 tattttagga attatcagtg ctatgttgac ttttaaagac aagcaaatca gcgcctcatt
    28081 aaagtttagc gcattgttaa ttaatgtgct gccattatgc ggtacctttg tagcttcaaa
    28141 ttaattgtcg atagcgcgtt tgaataaagt acagtgcatt aacagaagaa atatgatcgt
    28201 gttaatgaca ttgatgaaaa taaagcacat caacataacc tctcacttgc atagtagtga
    28261 gggggttttt gttggtttat ataaaatgaa gtaattaata tttaatgaaa attaagacat
    28321 gataaaattc taacggcagt atagatattt aaaaatgtgt atcaatggcg ctatagagat
    28381 aagcaagctt agtggtatca tatataaatt tgtaattttt attgcatagt gtatgcaagc
    28441 atgtttttta tatgcaaaat aggtagctaa atatgttaaa ctgtttgtaa caatttttat
    28501 cattaagaca gatataataa ataaaatata gaaaactgat taaaaggtat acagttgctc
    28561 gtatgtgtgt ccaactgtat ttagagtcgc acttgttgta aagcaaaata gttcgactta
    28621 cttagaagtg aaaacgataa gggtctggga cgtaaattaa tgtcctgggc gctacaatgt
    28681 tataggaaat gcgattaaaa tcgcagtgaa caaagggtat acagttgttc gcatacgcgt
    28741 ccaactgcat aagaaacact aaaagaatgt aattcatagt gtttctaaaa tttattcagg
    28801 ttaaggagta ttaagatgac agcagaaacg aattattttt ggttgaattg tggatataac
    28861 cgttggaatc ataatgaacc gttagtagga caaacagctt tatttgaatc aggcgcacat
    28921 tttaacccat ctcaaggatt tagagcgttt aaaaaggcta aagtaggaga tcaagtcatc
    28981 ttttatcaag ttcagacgga tactggacta cttggatgtg gtgaaattat tagtgttgaa
    29041 acaggtgcac aaaataaaat acgtgtacag tttcgtttta atgaacaatt gaaaccatta
    29101 acagcagatt atttaaagcg tagtgaagct ttggaatttc gtatgagtaa tatgaaagag
    29161 acattgttca atcaaatcac tgcagaagag tttgatttaa taagtggtct tggtaaaggc
    29221 gaaattaaga taccgcgtta tttcttttta gctgaaactg aagaatttga acctggaaac
    29281 caatatacga tttatacgca cacgtacaac ggtattaaac gtaatggtta tcatttttat
    29341 actcagttag aagaaggcga caatattatt ttttataatc gaacgaaaaa tcaatctgtc
    29401 gttggaattg gagaagtatc aaagcatatt catgaaaaac caccaattcc tggtagaaca
    29461 aatagtacag tgattgaagt gtcttatgag aaagacatta caccgattac attaagtaca
    29521 ttaaataagc atcctaaatt gaaaaactta tatttccttc aagaaaatgc aaaacaggct
    29581 attgcaagta tgtcacaagc acagtatgat gcaattattg aaatgagcga taataatgga
    29641 ttgaaatctc catttgaaat ggttcaaaaa ccagatatgc ttgaaagtga aaaagaagaa
    29701 gcgttaaagc catttatttt acttgttgta gatcgaaaag aagaaggctt aaaagcggct
    29761 aatgacttat tgcaaaaagc gaatgcaaat cctgttataa caacaggtca tcctgatttt
    29821 tcagaagata tgctttatgg caagtattta ccaaatgaaa caggggcatt atactatcgt
    29881 gaagggttta ttactcaatt aatgcctaaa aaggataaaa gttatttagt tattgataat
    29941 tttaatcgta tagatacaga cattttccaa acgtatatta atgtgttaga aggctatgaa
    30001 gtaacattac cacgttataa taaagacggt aatatgatta aatggtcacg acaaaaagat
    30061 tctttctatt atttcaatcc taattggcat attgtaggta ttacatatga cagtttagag
    30121 gaaattaagg agaaatattc agagcagttt ttaaaataca cacgtatagt caaagtcaaa
    30181 catgactaat gatatatggt agtttttgaa aagaagtaag gataggatta aaaattgcat
    30241 aattgcatat ttgaataacc gcataaagaa aaaatgacgg gtaatcaacc ataag
//
LOCUS       DAMZKC010000014        29835 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.014, whole genome shotgun sequence.
ACCESSION   DAMZKC010000014 DAMZKC010000000
VERSION     DAMZKC010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 29835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29835
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.014"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            97..771
                     /locus_tag="POB55_000452"
     CDS             97..771
                     /locus_tag="POB55_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDI6499447.1"
                     /translation="MIDKKLTSPKMTVPLFLIALIVFIGMFYSVVTNQEWLKNIDMGS
                     LTWFTDYFGEPQRQYVNNLFNYYMTFSAEIGDVKGVVLISIIVTIILFIKQRHLAVWF
                     VTYLVSGVIMNKLIKDTVLRPRPYNHLAVDTGFSFPSGHSNASTLLYFALMIIIISLA
                     AKTITKVLSALVMGILWLSILFCRLYFHAHYFSDVIGGTSLAIIWVALFLMVYPYFIN
                     HRRQRV"
     gene            808..1542
                     /locus_tag="POB55_000453"
     CDS             808..1542
                     /locus_tag="POB55_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase"
                     /protein_id="HDI6499448.1"
                     /translation="MRIKTPSPSYLKGTNGHAILLLHSFTGTNRDVKHLAAELNDQGF
                     SCYAPNYPGHGLLLKDFMTYNVDDWWEEVEKAYQFLVNEGYESISATGVSLGGLMTLK
                     LAQHYPLKRIAVMSAPKEKSDDGLIEHLVYYSQRMSDILNLDQQASSAQLAAIDDYEG
                     EITKFQHFIDDIMTNLNVIKMPANILFGGKDAPSYETSAHFIYEHLGSVDKELNGLKD
                     SHHLMTHGEGRDILEENVIRFFNALT"
     gene            1720..1833
                     /locus_tag="POB55_000454"
     CDS             1720..1833
                     /locus_tag="POB55_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499449.1"
                     /translation="MGIAPEVVMWRDVGDIDGVVCGEILAHVYSVEPGILM"
     gene            1998..3335
                     /locus_tag="POB55_000455"
     CDS             1998..3335
                     /locus_tag="POB55_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HDI6499450.1"
                     /translation="MKRQQSQWKSSTGFILASAGSAIGLGAMWKFPYMAGIYGGGAFL
                     AMFLIFTIFVGLPLLIMEFTVGKMGRTYTTQIYSKLTGKKWLNIIGWNGNLAVFILFG
                     FYSVIGGWIVIYIGQVLWQLVIFQRINHLQEMNFEAVISNPWLTVLGQGIFIFATMII
                     VMLGVEKGLEKASKVMMPLLFVFLIIIVIKSLTLDGALEGVKFILQPRVSEITADGIL
                     FALGQSFFTLSLGTTGMITYASYASKDMTIKSSAISIVVMNIFVSVLAGLAIFPALHS
                     FGYEPQEGPGLLFKVLPMVFSQMHLGTLFYLGFLVLFLFAALTSSISLLELNVSNFTK
                     NDNTKRKKVAVIGSILVFIISIPATLSFGILKDVRFGAGTIFDNMDFIVSNVLMPLGA
                     LGTTLVVGQLLDKKLLQQYFGKDRFRLFSGWYYLIKYAMPVVIILVFIVQLFS"
     gene            3552..4457
                     /locus_tag="POB55_000456"
     CDS             3552..4457
                     /locus_tag="POB55_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HDI6499451.1"
                     /translation="MITYDLIGNTPLVLLEHYSDDKVKIYAKLEQWNPGGSVKDRLGK
                     YLVEKAIQEGRVRAGQTIVEATAGNTGIGLAIAANRHHLKCKIFAPYGFSEEKINIMI
                     ALGADVSRTSQSEGMHGAQLAARSYAEKYGAVYMNQFESEHNPDTYFHTLGPELTSAL
                     QQIDYFVAGIGSGGTFTGTARYLKQHHVQCYAVEPEGSVLNGGPAHAHDTEGIGSEKW
                     PIFLERRLVDGIFTIKDQDAFRNVKSLAINEGLLVGSSSGAALQGALNLKAQLSEGTI
                     VVVFPDGSDRYMSKQIFNYEENDYE"
     gene            4450..5592
                     /locus_tag="POB55_000457"
     CDS             4450..5592
                     /locus_tag="POB55_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional cystathionine
                     gamma-lyase/homocysteine desulfhydrase"
                     /protein_id="HDI6499452.1"
                     /translation="MNKKTKLIHGGHTTDDYTGAVTTPIYQTSTYLQDDIGDLRQGYE
                     YSRTANPTRSSVESVITALENGKHGFAFSSGVAAISAVVMLLDKGDHIILNSDVYGGT
                     YRALTKVFTRFGIEVDFVDTTHTDSIVQAIRPTTKMLFIETPSNPLLRVTDIKKSAEI
                     AKEHGLISVVDNTFMTPYYQNPLDLGIDIVLHSATKYLGGHSDVVAGLVATSDDKLAE
                     RLAFISNSTGGILGPQDSYLLVRGIKTLGLRMEQINRSVIEIIKMLQAHPAVQQVFHP
                     SIESHLNHDVHMAQADGHTGVIAFEVKNTESAKQLIKATSYYTLAESLGAVESLISVP
                     ALMTHASIPADIRAKEGITDGLVRISVGIEDTEDLVDDLKQALDTL"
     gene            5889..6914
                     /locus_tag="POB55_000458"
     CDS             5889..6914
                     /locus_tag="POB55_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HDI6499453.1"
                     /translation="MIEFRQVSKTFNKKKQKIDALKDVSFTVNRNDIFGVIGYSGAGK
                     STLVRLVNHLEAASNGQVIVDGHDITNYSDKMMRDIKKDIGMIFQHFNLLNSATVFKN
                     VAMPLILSKKSKTEIKQRVTEMLEFVGLSDKKDQFPEELSGGQKQRVAIARALVTNPK
                     ILLCDEATSALDPATTASILTLLKNVNQTFGITIMMITHEMRVIKDICNRVAVMEKGQ
                     VVETGTVKEVFSHPKTTIAQNFVSTVIQTEPSTSLIRRLNDEQVGDFKDYKIFVEETQ
                     VTQPIINDLIQICGREVKILFSSMSEIQGNTVCYMWLRFNMDQQFDDTAINQYFKEKN
                     IQFEEVH"
     gene            6918..7577
                     /locus_tag="POB55_000459"
     CDS             6918..7577
                     /locus_tag="POB55_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6499454.1"
                     /translation="MFGSDLDSAQLLQALYETLYMVSIALFLGAVIGIPLGVLLVITR
                     KQGIWPNIVIHQVLNPIINILRSLPFIILLIAIVPFTKLVVGTSIGTTAAIVPLTVYV
                     APYIARLVENSLLEVDEGIIEAAKAMGASPLQIIRYFLIPEALGSLVLAITTAIIGLI
                     GSTAMAGAVGGGGIGDLALVYGYQRFDTTVIIITVIVLVIIVQVIQTLGNVLARFIRR
                     H"
     gene            7614..8456
                     /gene="gmpC"
                     /locus_tag="POB55_000460"
     CDS             7614..8456
                     /gene="gmpC"
                     /locus_tag="POB55_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter
                     glycylmethionine-binding lipoprotein"
                     /protein_id="HDI6499455.1"
                     /translation="MKRLIGLVIVALVLLAACGSNNDKKVTIGVASNDTKAWEKVKEL
                     AKKDDIDVEIKHFSDYNLPNKALNDGDIDMNAFQHFAFLDQYKKAHKGTKISALSTTV
                     LAPLGIYSDKIKDVKKVKDGAKVVIPNDVSNQARALKLLEAAGLIKLKKDFGLAGTVK
                     AITSNPKHLKITAVDAQQTARALSDVDIAVINNGVATKAGKDPKNDPIFLEKSNSDAV
                     KPYINIVAVNDKDLDNKTYAKIVELYHSKEAQKALQEDVKDGEKPVNLSKDEIKAIET
                     SLAK"
     gene            8788..9792
                     /gene="aaa"
                     /locus_tag="POB55_000461"
     CDS             8788..9792
                     /gene="aaa"
                     /locus_tag="POB55_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin/adhesin Aaa"
                     /protein_id="HDI6499456.1"
                     /translation="MQKKVIAAIIGTSAISAVAATQANAATTHTVKPGESVWAISNKY
                     GISIAKLKSLNNLTSNLIFPNQVLKVSGSSNSTSNSSRPSTNSGGGSYYTVQAGDSLS
                     LIASKYGTTYQNIMRLNGLNNFFIYPGQKLKVSGTASSSNAASNSSRPSTNSGGGSYY
                     TVQAGDSLSLIASKYGTTYQKIMSLNGLNNFFIYPGQKLKVTGNASTNSGSATTTNRG
                     YNTPVFSHQNLYTWGQCTYHVFNRRAEIGKGISTYWWNANNWDNAAAADGYTIDNRPT
                     VGSIAQTDVGYYGHVMFVERVNNDGSILVSEMNYSAAPGILTYRTVPAYQVNNYRYIH
                     "
     gene            complement(9977..10246)
                     /locus_tag="POB55_000462"
     CDS             complement(9977..10246)
                     /locus_tag="POB55_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499457.1"
                     /translation="MAKRSKSQRLSSLLNVAGFIVDGYNGYKYHAKNKKLVYLSLGLS
                     TVGTVLDFYISIKSPRKFKKAVAVVTLITNGARLFTSIRKVKHEY"
     gene            10395..10790
                     /locus_tag="POB55_000463"
     CDS             10395..10790
                     /locus_tag="POB55_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HDI6499458.1"
                     /translation="MIKCVCLVEETADKILLVQVRNREKYYFPGGKIEEGESRVQALL
                     REVKEELNLTLTMDEIEYIGTIVGPAYPQQDMLTELNGFRALTKIDWENVTINNEITD
                     IRWIDKDNDALIAPAVKVWIETYGGKHDK"
     gene            10780..11265
                     /locus_tag="POB55_000464"
     CDS             10780..11265
                     /locus_tag="POB55_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6499459.1"
                     /translation="MTNNDTIMLRHYVPQDYSMLEAFQLSESDLKFVKTPEENITAAM
                     SDNERYPIVVMDGRQCVAFFTLHRGKGVAPFSDNQDAVFFRSFSVDQRYRNRGIGKVV
                     MEKLASFITSTFQDINEIVLTVNTDNPHAMALYRQQGYQYMGDSMFIGRPVHIMALTI
                     K"
     gene            complement(11434..12216)
                     /locus_tag="POB55_000465"
     CDS             complement(11434..12216)
                     /locus_tag="POB55_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HDI6499460.1"
                     /translation="MNAVFILALLLLFLMIIFGGRKGFISYLTLFLNFAILIISIVLI
                     IFGVPIYVVTFFFCIIIGACNLFVLNSYNVKTQAAFISTIVTTILLITLIYLSVYFGH
                     LQGFSTEQQDETYVYSMNIGIDMVQFMVFTIILAVIAAVIDLTITISSPIYELHQVNP
                     TLTQYELFQSGMRVGREILATSANTIYLAFFGGQLALFIWFFKLKYSFGHIINSKIFA
                     QEFISIILGGIAVAISIPITAWITAFLIKRPSRQRKNDESIN"
     gene            complement(12213..13331)
                     /locus_tag="POB55_000466"
     CDS             complement(12213..13331)
                     /locus_tag="POB55_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HDI6499461.1"
                     /translation="MFLKYRNLFTKPFNIILGLFCIAFIGAILFTFNNENFYNKPIGQ
                     IIDVKHVSSTPTKDAQNNRDIKYKNQLKVKILNGQFAGETTTINHQYVKSQADSEAFR
                     THEKVLLHISNKPSDAYIIEKKRDTLTVIITGLFLLTVLLVGRKVGLQSILSLILNSI
                     AILIAIYIHIQHSNINLFLLMTIAMICSTILTLLLVTGWHMRTLITIASTIIGTFLSI
                     GLTELIIYMTDGKGIKYETMNFLSLPPKDIFLASVLIGSLGAIMDVAITIASGMHEIL
                     QRTPHISMRRWALAGRNIGQDIMGTMTNILLFSYLSGALPMFLIFLKNANTVTYTISM
                     NWSLEIARALTGGIGIVLTIPITILFMEIFETLRRAKQ"
     gene            complement(13446..14329)
                     /locus_tag="POB55_000467"
                     /pseudo
     CDS             complement(13446..14329)
                     /locus_tag="POB55_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499017.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            14511..19010
                     /gene="gltB"
                     /locus_tag="POB55_000468"
     CDS             14511..19010
                     /gene="gltB"
                     /locus_tag="POB55_000468"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HDI6499462.1"
                     /translation="MHNEKLIKGLYDYREEHDACGIGFYANMDNKRSHDIIDKSLEML
                     RRLDHRGGVGADGITGDGAGIMTEIPFAFFKQHVTDFDIPGEGEYAVGLFFSKERILG
                     SEHEAVFKKYFEGEGLSILGYRNVPVNKDAIAKHVADTMPVIQQVFIDIRDIEDVEKR
                     LFLARKQLEFYSTQCDLELYFTSLSRKTIVYKGWLRSDQIKKLYTDLSDDLYQSKLGL
                     VHSRFSTNTFPSWKRAHPNRMLMHNGEINTIKGNVNWMRARQHKLIETLFGEDQHKVF
                     QIVDEDGSDSAIVDNALEFLSLAMEPEKAAMLLIPEPWLYNEANDANVRAFYEFYSYL
                     MEPWDGPTMISFCNGDKLGALTDRNGLRPGRYTITKDNFIVFSSEVGVVDVPESNVAF
                     KGQLNPGKLLLVDFKQNKVIENNDLKGAIAGELPYKAWIDNHKVDFDFENIQYQDSQW
                     KDETLFKLQRQFAYTKEEIHKYIQELVEGKKDPIGAMGYDAPIAVLNERPESLFNYFK
                     QLFAQVTNPPIDAYREKIVTSELSYLGGEGNLLAPDETVLDRIQLKRPVLNESHIAAI
                     DQEHFKLTYLSTVYEGDLEDALEVLGREAVNAVKQGAQILVLDDSGLVDSNGFAMPML
                     LAISHVHQLLIKADLRMSTSLVAKSGETREVHHVACLLAYGANAIVPYLAQRTVEQLT
                     LTEGLQGTVVDNVKTYTDVLSEGVIKVMAKMGISTVQSYQGAQIFEAIGLSHDVIDRY
                     FTGIQSKLSGISIDQIDAENKARQQSDDNYLASGSTFQWRQQGQHHAFNPESIFLLQH
                     ACKENDYAQFKAYSEAVNKNRTDHIRHLLEFKACTPIDIDQVEPVSDIVKRFNTGAMS
                     YGSISAEAHETLAQAMNQLGGKSNSGEGGEDAKRYEVQVDGSNKVSAIKQVASGRFGV
                     TSDYLQHAKEIQIKVAQGAKPGEGGQLPGTKVYPWIAKTRGSTPGIGLISPPPHHDIY
                     SIEDLAQLIHDLKNANKDADIAVKLVSKTGVGTIASGVAKAFADKIVISGYDGGTGAS
                     PKTSIQHAGVPWEIGLAETHQTLKLNDLRSRVKLETDGKLLTGKDVAYACALGAEEFG
                     FATAPLVVLGCIMMRVCHKDTCPVGVATQNKDLRALYRGKAHHVVNFMHFIAQELREI
                     LASLGLKRVEDLVGRTDLLQRSSTLKVNSKAASIDVEKLLCPFDGPNTKEIQQNHNLE
                     HGFDLTNLYEVTKPYIAEGRRYTGSFTVNNEQRDVGVITGSEISKQYGEAGLPENTIN
                     VYTNGHAGQSLAAYAPKGLMIHHTGDANDYVGKGLSGGTVIVKAPFEERQNEIIAGNV
                     SFYGATGGKAFINGSAGERFCIRNSGVDVVVEGIGDHGLEYMTGGHVINLGDVGKNFG
                     QGMSGGIAYVIPSDVEAFVENNQLDTLSFTKIKHQEEKAFIKQMLEEHVSHTNSTRAI
                     HVLKHFDRIEDVVVKVIPKDYQLMMQKIHLHKSLHDNEDEAMLAAFYDDSKTIDAKHK
                     PAVVY"
     gene            19028..20491
                     /locus_tag="POB55_000469"
     CDS             19028..20491
                     /locus_tag="POB55_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HDI6499463.1"
                     /translation="MGEFKGFMKYDKQYLGELSLVDRLKHHKAYQQRFTKEDASIQGA
                     RCMDCGTPFCQTGQQYGRETIGCPIGNYIPEWNDLVYHQDFKTAYERLSETNNFPDFT
                     GRVCPAPCESACVMKINRESIAIKGIERTIIDEAFENGWVAPKVPSRRRDEKVAIVGS
                     GPAGLAAAEELNLLGYQVTIYERARESGGLLMYGIPNMKLDKDVVRRRIKLMEEAGIT
                     FINGVEVGVDIDKATLESEYDAIILCTGAQKGRDLPLEGRMGDGIHFAMDYLTEQTQL
                     LNGEIDDITITAKDKNVIIIGAGDTGADCVATALRENCKSIVQFNKYTKLPEAITFTE
                     NASWPLAMPVFKMDYAHQEYEAKFGKEPRAYGVQTMRYDVDDKGHIRGLYTQILEQGE
                     NGMVMKEGPERFWPADLVLLSIGFEGTEPTVPNAFNIKTDRNRIVADDTNYQTNNEKV
                     FAAGDARRGQSLVVWAIKEGRGVAKAVDQYLASKVCV"
     gene            20760..20852
                     /locus_tag="POB55_000470"
     tRNA            20760..20852
                     /locus_tag="POB55_000470"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20796..20798,aa:Ser,seq:tga)
     gene            21322..22749
                     /gene="treP"
                     /locus_tag="POB55_000471"
     CDS             21322..22749
                     /gene="treP"
                     /locus_tag="POB55_000471"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HDI6499464.1"
                     /translation="MAVKREDVKAIVSAIGGKENLEAATHCVTRLRLVLKDESKVDKD
                     ALSNNALVKGQFKADHQYQIVIGPGTVDEVYKQFIDETGAQEASKDEAKQAAAQKGNP
                     VQRLIKLLGDIFIPILPAIVTAGLLMGINNLLTMKGLFGPKALIEMYPQIADISNIIN
                     VIASTAFIFLPALIGWSSMRVFGGSPILGIVLGLILMHPQLVSQYDLAKGNIPTWNLF
                     GLEIKQLNYQGQVLPVLIAAYVLAKIEKGLNKVVHDSIKMLVVGPVALLVTGFLAFII
                     IGPVALLIGTGITSGVTFIFQHAGWLGGAIYGLLYAPLVITGLHHMFLAVDFQLMGSS
                     LGGTYLWPIVAISNICQGSAAFGAWFVYKRRKMVKEEGLALTSCISGMLGVTEPAMFG
                     VNLPLKYPFIAAISTSCVLGAIVGMNNVLGKVGVGGVPAFISIQKEFWPVYLIVTAIA
                     IVVPCILTIVMSHFSKQKAKEIVED"
     gene            22813..24453
                     /gene="treC"
                     /locus_tag="POB55_000472"
     CDS             22813..24453
                     /gene="treC"
                     /locus_tag="POB55_000472"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HDI6499465.1"
                     /translation="MSKEIDWRKSVVYQIYPKSFNDTTGNGIGDINGIIEKLDYIKLL
                     GVDYIWLTPVYESPMNDNGYDISNYLEINEDFGTMDDFEKLIKVAHQKDLKVMLDIVI
                     NHTSTEHEWFKEARKSKDNPYRDYYFFRSSEDGPPTNWHSKFGGNAWKFDSETDEYYL
                     HLFDVSQADLNWDNPEVRQSLYRIVNHWIDFGVDGFRFDVINLISKGEFKDSDKIGKE
                     FYTDGPRVHEFLHELNRQTFGNTDMMTVGEMSSTTIENCIKYTQPERQELNSVFNFHH
                     LKVDYVDGEKWTNAKLDFHKLKKILMQWQRGIYDGGGWNAIFWCNHDQPRVVSRFGDD
                     TSEEMRIQSAKMLAIALHMLQGTPYIYQGEEIGMTDPHFTSIAQYRDVESINAYHQLL
                     SEGHAEADVLTILGQKSRDNSRTPMQWSDDVNAGFTAGKPWIDISENYHQVNVRQALQ
                     NKESIFYTYQKLIQLRHTHDIITYGDIVPRFMDHDHLFVYERHYKNQQWLVIANFSAS
                     AVDLPEGLAREGRVVIQTGTVENNTISGFGAIVIETNA"
     gene            24478..25206
                     /gene="treR"
                     /locus_tag="POB55_000473"
     CDS             24478..25206
                     /gene="treR"
                     /locus_tag="POB55_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HDI6499466.1"
                     /translation="MAKQKKFMKIYEALKEDILNGQIQYGEQIPSEHDLVQLYQSSRE
                     TVRKALDLLALDGMIQKIHGKGSLVIYQEVTEFPFSELVSFKEMQEEMGVAYLTEVVV
                     NEVVEAHEVPEVQHALNINSSESLIHIVRTRRLNQHVKIVDEDYFLKSIVSDIGNDVA
                     SDSIYDYLEKVLNLNISYSSKSITFEPFDEQAYQLFGDVSVAYSATVRSIVYLENTMP
                     FQYNISKHLANEFKFNDFSRRRIK"
     gene            25268..25537
                     /gene="ffs"
                     /locus_tag="POB55_000474"
     ncRNA           25268..25537
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="POB55_000474"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            25610..25711
                     /locus_tag="POB55_000475"
     CDS             25610..25711
                     /locus_tag="POB55_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499467.1"
                     /translation="MKTIQRIIRGTCLWEVAFLYVKFDSSEIDVQFE"
     gene            25849..26373
                     /locus_tag="POB55_000476"
     CDS             25849..26373
                     /locus_tag="POB55_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDI6499468.1"
                     /translation="MQIYLSTLTELDYDKSLNSIEESFDDNPETSWQALAKVKHLRKS
                     PCYNFELEVIAKNENNDVVGHVLLIEVEINSDDKTYYGLAIASLSVHPELRGQKLGRG
                     LVQAVEERAKAQEYSTVVVDHCFDYFEKLGYQNAAEHDIKLESGDAPLLVKYLWDNLT
                     DAPHGIVKFPEHFY"
     gene            26442..28139
                     /gene="dnaX"
                     /locus_tag="POB55_000477"
     CDS             26442..28139
                     /gene="dnaX"
                     /locus_tag="POB55_000477"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HDI6499469.1"
                     /translation="MNYQALYRMYRPQSFEDVVGQEHVTKTLRNAISKEKQSHAYIFS
                     GPRGTGKTSIAKVFAKAINCLNSTDGEPCNECHICKGITQGTNSDVIEIDAASNNGVD
                     EIRNIRDKVKYAPSELKYKVYIIDEVHMLTTGAFNALLKTLEEPPAHAIFILATTEPH
                     KIPPTIISRAQRFDFKAISLNQIVERLKFVADAQQIECEDEALAFIAKASEGGMRDAL
                     SIMDQAIAFGDGTLTLQDALNVTGSVHDEALDHLFDDIVQGDVQASFKKYHQFITEGK
                     EVNRLINDMIYFVRDTIMNKTSEKDTEYRALMNLELDMLYQMIDLINDTLVSIRFSVN
                     QNVHFEVLLVKLAEQIKGQPQVIANVAEPAQIASSPNTDVLLQRMEQLEQELKTLKAQ
                     GVSVAPVQKSSKKPARGIQKSKNAFSMQQIAKVLDKANKADIKLLKDHWQEVIDHAKN
                     NDKKSLVSLLQNSEPVAASEDHVLVKFEEEIHCEIVNKDDEKRSSIESVVCNIVNKNV
                     KVVGVPSDQWQRVRTEYLQNRKNEGDDMPKQQAQQTDIAQKAKDLFGEETVHVIDEE"
     gene            28229..28546
                     /locus_tag="POB55_000478"
     CDS             28229..28546
                     /locus_tag="POB55_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002479279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HDI6499470.1"
                     /translation="MRGGGNMQQMMKQMQKMQKKMAQEQEKLKEERIVGTAGGGMVAV
                     TVTGHKEVVDVEIKEEAVDPDDIEMLQDLVLAATNEAMNKADELTQERLGKHTQGLNI
                     PGM"
     gene            28553..29149
                     /gene="recR"
                     /locus_tag="POB55_000479"
     CDS             28553..29149
                     /gene="recR"
                     /locus_tag="POB55_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009383386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HDI6499471.1"
                     /translation="MHYPEPISKLIDSFMKLPGIGPKTAQRLAFHTLDMKEDDVVQFA
                     KALVDVKRELTYCSVCGHITENDPCYICEDKQRDRSVICVVEDDKDVIAMEKMREYKG
                     LYHVLHGSISPMDGIGPEDINIPSLIERLKNDEVSELILAMNPNLEGESTAMYISRLV
                     KPIGIKVTRLAQGLSVGGDLEYADEVTLSKAIAGRTEM"
     gene            29354..29452
                     /locus_tag="POB55_000480"
     CDS             29354..29452
                     /locus_tag="POB55_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499472.1"
                     /translation="MNWIIELDLWLWCVGAINLINAVNDYANEILL"
BASE COUNT         9905 a         4264 c         6275 g         9391 t
ORIGIN      
        1 ataattttga tattgtcggt aaagtgaaat ttgtggcaca ttgatattat ggataacatt
       61 tgtaaaatga aaatgatgat acgaggagat tgtaagatga tagataaaaa attaacatca
      121 ccgaaaatga cagtgccttt atttttaatc gcgctgattg tatttatagg tatgttttac
      181 agtgtagtga caaatcaaga atggcttaaa aatatagata tgggatcatt aacatggttt
      241 acagattatt tcggtgagcc acaacgtcag tatgttaaca atttatttaa ttactatatg
      301 acgtttagtg cggaaattgg agatgtcaaa ggtgtcgtgt tgatttccat tatcgtcaca
      361 atcatactgt ttattaaaca gaggcattta gcggtttggt ttgtgacata tttggtttca
      421 ggtgtcatca tgaacaaatt aattaaagat actgttttac gtccaagacc atataatcat
      481 ttagccgttg atacaggctt ttcatttcca agtggacatt ccaacgccag cacattatta
      541 tatttcgctt taatgatcat aattatttca cttgctgcta agacaataac aaaagtgttg
      601 agtgcgctag ttatgggaat attatggctt agcatattat tttgtcgcct ttattttcat
      661 gcgcattact tttcagatgt cattggcggc acgtcactag caatcatttg ggtagcgtta
      721 ttcttaatgg tatacccata ctttattaat catcgacgac aacgcgttta gcaaacatct
      781 ttagacatag tggaggtata tagaaaaatg agaattaaaa caccgagtcc ctcttattta
      841 aaaggcacaa atggacatgc gatattatta ttacattcat ttacaggtac aaatcgggat
      901 gtgaagcatc ttgcagctga gttaaatgac caaggattta gttgttatgc accgaattat
      961 ccaggtcatg gcttactgtt gaaagatttc atgacatata atgtagatga ttggtgggaa
     1021 gaagttgaga aagcttacca atttttagtc aatgaaggtt atgaatctat cagtgcaacg
     1081 ggtgtgtctt taggtggatt aatgacatta aaattggcgc aacactatcc tttgaaacgt
     1141 atcgctgtca tgtcagcacc aaaggaaaag agtgacgatg gtttaataga acatttagtt
     1201 tattatagtc aacgcatgtc ggatatttta aatttagatc agcaagcatc gagtgcgcaa
     1261 ttagcagcaa ttgatgatta tgaaggtgaa attacgaagt ttcaacattt tattgatgat
     1321 atcatgacaa atttaaatgt tattaaaatg ccagctaata tattatttgg tggtaaagat
     1381 gcgccatcct atgaaacaag tgcacatttt atttatgaac atttaggatc agtagacaaa
     1441 gaattaaatg gtctgaagga ttcgcatcat ttaatgacgc atggagaagg cagagatatt
     1501 ttagaagaaa atgttattcg ctttttcaat gctttaacat aattgaagat tttaaaaatt
     1561 aacacttgag tgacgatgtt attatatttg tcatttgagt gttttttgtg gcgtgatagt
     1621 atagggaact tagctaaagt taatttttag tgtgatgtcg tggaaatatt atggagttat
     1681 accagaagtt gatttgtatt gtaatgttgt gggcatttta tgggcattgc acctgaagtt
     1741 gttatgtggc gagatgttgg ggatatagat ggtgttgtat gtggcgagat attggcgcac
     1801 gtatacagcg ttgaaccagg aattttaatg tagcgtaatg acaacacatt acaagagttg
     1861 actatattat gataaatcca ggcgctaccg tattaatgaa acaattttga tgcaggtgta
     1921 ttgcatgtta tttacataca tgttagagtg ttacttaaac acgtagacgc tttaacgctt
     1981 taaaggagat gtgaatgatg aaaagacaac aatcacaatg gaagtcatca actggattta
     2041 ttttagctag tgcgggttct gcaatcggtc ttggtgccat gtggaaattc ccatatatgg
     2101 cagggattta tggcggcggt gcctttctag ctatgttctt aatattcacc atttttgttg
     2161 ggttaccatt actcattatg gaattcactg ttgggaaaat gggacggaca tatacaacac
     2221 aaatatatag taaattaact ggtaaaaaat ggctcaatat cattggctgg aacggtaatt
     2281 tggccgtgtt tattttattt ggcttctata gtgttatcgg tggttggatt gtcatttaca
     2341 tcggacaagt attatggcaa ttagttatat ttcaacgcat caatcatctc caagaaatga
     2401 attttgaagc ggtaatatca aatccttggt taactgttct agggcaaggt atattcatat
     2461 tcgctacgat gattattgtc atgttaggtg ttgaaaaagg attagaaaag gcatcgaaag
     2521 ttatgatgcc attgctgttt gtctttttaa tcatcattgt gattaagtct ttaacattag
     2581 atggcgcctt agaaggtgtg aaatttattt tacaaccaag agtgtcagag attactgctg
     2641 acggtatctt gtttgcgcta ggccaatcat tctttacgtt atcattagga actacaggta
     2701 tgattactta tgcgagttat gcctctaaag acatgacgat taagtcatca gctatttcta
     2761 tcgttgttat gaatatcttt gtatctgtat tggcaggtct agctatattt ccggcgttac
     2821 atagttttgg ctatgaacca caagaagggc ctggattatt atttaaagta ctgccaatgg
     2881 tctttagtca aatgcatcta ggcacattat tctatttggg attcttagtg ctgttcttat
     2941 ttgcggcttt aacgtcatct atttctttat tagaattaaa tgtttctaac ttcacgaaga
     3001 atgacaatac aaaacgtaaa aaagtcgcag tgatcggtag tattttagta tttatcatta
     3061 gtattccagc aaccttatct tttggtatct taaaagatgt aagattcggt gcgggaacga
     3121 tttttgataa tatggatttc atcgtttcga atgtattgat gccattaggc gcattaggta
     3181 ctacgcttgt cgtaggacaa ttattagata aaaaattatt acaacaatat tttggtaaag
     3241 atcgatttag attattcagt ggttggtatt acttaattaa gtatgcgatg cctgtcgtta
     3301 ttattttagt ctttatcgtg caattattta gttaatatat agaagcatct gacacacaag
     3361 tatttgtgtt gctcaggtgt ttttttgttt ggaattgaaa agttagtact ggttctgaat
     3421 taatcgaaac ttagataatt gcaaaataac aaaatgtcgt ttataattct tatctgaaaa
     3481 gtaagaatta aaattgagac atgaagaaag gtttcattca tttgatcggc atgtcagatg
     3541 agggtgcatc tatgattact tatgatttaa ttggcaatac accattagta ctgttagaac
     3601 attatagtga tgataaagtt aaaatttatg ccaagcttga acaatggaat cctggaggca
     3661 gtgttaaaga cagactcggg aaatatttag tagagaaggc aattcaagaa gggcgtgtgc
     3721 gtgcaggtca aactattgtt gaagcgactg ctggtaatac aggcataggg ttagctattg
     3781 cagcgaatag acatcattta aaatgtaaga tctttgcgcc gtatggtttt tcagaagaaa
     3841 agattaatat tatgatagcg cttggtgcag atgtttcaag gacgagtcag tctgaaggta
     3901 tgcatggggc acaattagct gcacgttcct atgctgaaaa atatggtgcc gtttatatga
     3961 atcaatttga atccgaacat aatccggata catattttca tacattggga cccgaattga
     4021 cttcagcatt acagcaaatt gattattttg tggctggtat tggctctggc ggtacattta
     4081 caggtaccgc acgttattta aagcaacatc acgtgcaatg ttatgccgtt gagccagaag
     4141 ggtccgtgtt aaatggaggg ccagctcatg cacatgacac tgaaggtatc ggttctgaga
     4201 aatggccgat atttttagag agacgtcttg tagatgggat atttacgatt aaagatcaag
     4261 atgcctttcg aaatgtcaaa agtttggcta taaatgaagg gttgttagta ggcagttctt
     4321 caggtgcagc attacaaggt gcattgaatt taaaagcgca attatctgaa ggtacgattg
     4381 ttgtcgtatt tccagatggt agcgatcgat atatgtctaa gcaaatattt aattatgagg
     4441 agaatgatta tgaataagaa aactaaatta attcacggtg gacacacaac ggacgactat
     4501 acaggtgcag ttactacacc aatttatcaa acaagtacat atttacaaga tgatattggt
     4561 gatttacgtc aaggatatga atattctcgt actgcgaatc caacaagaag ttctgtagaa
     4621 agcgttatta cggcattaga aaatggcaaa catggctttg cgtttagttc aggtgttgca
     4681 gctatcagtg cagttgttat gctgttggac aaaggtgatc atattatttt aaattcagat
     4741 gtatacggcg gtacttatcg cgcattgaca aaagtattta cacgatttgg cattgaagtg
     4801 gattttgtag atacaacgca tacagattca attgtacaag cgatacgccc aacaacaaag
     4861 atgttgttta ttgaaacacc ttctaatcca ttattacgtg ttactgacat taaaaagtct
     4921 gctgaaattg cgaaagaaca cggtttgatt tcagtcgttg ataacacatt tatgacacct
     4981 tattatcaga atccattaga tttaggcatc gatattgtct tgcattctgc aacgaaatat
     5041 ttaggtggac atagtgatgt cgttgctggt ttagttgcaa catcggatga caagcttgca
     5101 gaacgtttag catttatttc aaattcaaca ggtggcattt taggacctca agatagctat
     5161 ttacttgtga ggggtattaa aacattaggt ttacgtatgg aacaaattaa tcgcagtgtt
     5221 attgaaatta ttaaaatgtt acaagcacat ccagctgtgc aacaagtgtt ccatccaagt
     5281 attgaaagtc atttaaatca tgatgtccat atggctcaag cggatggcca tacaggtgtg
     5341 attgcatttg aagtgaaaaa tacagaaagt gccaaacaat tgattaaagc aacatcgtat
     5401 tacacattag ctgaaagttt aggtgcagtg gaaagtttaa tttcagtacc tgcattgatg
     5461 acacatgcat ccattccagc agatattcga gctaaagaag gtattacaga cggacttgta
     5521 agaatttctg taggtattga agatactgaa gatttagtcg atgatttaaa acaagcacta
     5581 gatacgttat aaataatagc agcactggca tatattttga gttcaacgtt tgttgttctt
     5641 gaatatgcta gtgctttttt tgtgcgatgt taatcattcg tgattgagtt atggaggttt
     5701 ggtggacatt tgtatggatt taatttgata aatgtcatag tagtctcaca attcgtcatt
     5761 gtcacatgaa ttgcttattt tttaattttt tagaaaattc ggcaaattgt attgacgata
     5821 tatgcaattt tacatataat gcttttattc ctagtggaat gataagaatt tgaaggaggg
     5881 ggctttgcat gattgagttt cgacaggtaa gtaagacctt taataaaaag aagcaaaaaa
     5941 tagatgcttt gaaggacgta tcatttacgg tcaatcgcaa tgatattttt ggtgtgattg
     6001 gatatagtgg tgcaggaaaa agtacgttgg taagactcgt gaatcatctt gaagctgcct
     6061 cgaatggaca agtgattgta gatggacatg atattacgaa ttatagcgat aaaatgatga
     6121 gggatattaa gaaagatatc ggtatgatat ttcagcattt caatttatta aattcagcta
     6181 ccgtatttaa aaatgtagca atgccactca ttttaagtaa gaaaagcaaa acagaaatta
     6241 agcaacgagt aacggaaatg cttgaatttg taggattgag tgataaaaaa gaccaatttc
     6301 ctgaagaatt atctggtggg cagaagcaaa gggtggctat tgcaagagcg cttgttacta
     6361 atccgaaaat actcctatgc gatgaagcaa caagcgcatt ggatccagca acgactgctt
     6421 cgatattgac gttattaaag aatgtcaatc aaacctttgg cattacaatt atgatgatta
     6481 cacatgaaat gcgcgttatt aaagacattt gtaatcgtgt tgctgtaatg gaaaagggtc
     6541 aagtggttga aacaggaact gttaaagagg tgtttagtca tcctaaaacg acgattgctc
     6601 aaaattttgt gtctacagtt atacagactg agccaagtac atcattgatt cgtcgattga
     6661 atgacgaaca agttggcgat tttaaagatt ataaaatctt cgtcgaggaa actcaggtga
     6721 cacaaccgat tataaatgac ttgattcaaa tttgtggcag agaggttaaa attttatttt
     6781 catctatgtc agaaatacaa ggtaacaccg tatgttatat gtggcttcga tttaatatgg
     6841 atcaacaatt tgatgacacg gcaataaatc aatatttcaa agagaaaaat attcaatttg
     6901 aggaggtgca ttaacatatg tttggttctg atttagacag tgcacagtta ttacaagcat
     6961 tgtacgaaac gctatatatg gtatctattg ctttattttt aggagcagtg attggtattc
     7021 cattaggtgt cttattggta attactcgaa aacaaggcat atggccgaat atagtgatac
     7081 atcaagtttt aaatcctata atcaatattt taaggtcact accatttatt attttgttaa
     7141 ttgcgattgt accattcaca aaattagtag taggtacttc aattggtacg actgctgcca
     7201 tcgtgccttt aacagtatat gtggcacctt acattgcaag acttgttgaa aactcattat
     7261 tagaagtaga cgaggggatt attgaagcgg cgaaagcgat gggcgcttca ccactacaaa
     7321 tcattagata ttttttaatt cctgaagcgt taggttcatt agtattagca attaccactg
     7381 cgattattgg acttattgga agtacggcga tggcaggagc tgttggcggt ggtggtatag
     7441 gagacttagc tttagtgtat ggttatcaaa gatttgatac gacggtcatt attattaccg
     7501 ttattgtatt agtcattatt gtccaagtga ttcaaacgct agggaatgtc ctagctagat
     7561 tcatacgtag acattaatga tatatagtga agattttgaa aggaattgat agaatgaaaa
     7621 gattgattgg gttagttatc gtagcacttg tattattagc agcgtgtggt agtaacaatg
     7681 ataaaaaagt aacaattggt gtcgcatcaa atgacactaa ggcttgggag aaggttaaag
     7741 aattagctaa aaaagatgat attgatgtgg agattaagca cttttccgat tacaatttac
     7801 cgaataaagc attaaacgat ggtgatattg atatgaatgc attccaacat tttgcatttt
     7861 tagatcagta taagaaggca cataaaggaa caaagatttc agcattgagt acaacagttt
     7921 tagcaccgtt gggcatttac tcagataaaa ttaaagatgt caaaaaggtt aaagatggtg
     7981 ctaaagttgt cattccaaat gatgtatcaa accaagcacg tgcacttaaa ctattagaag
     8041 cagctggttt aataaaactg aaaaaagatt tcggattagc aggtacagtg aaagctataa
     8101 cgtcaaatcc aaaacattta aaaattactg cagtagatgc acagcaaact gcgcgtgctt
     8161 tatctgatgt cgatattgca gttattaata acggtgtagc aactaaagcg gggaaagacc
     8221 ctaaaaatga tccgatattt ttagaaaaat caaattcaga tgctgtaaag ccatatatta
     8281 atattgttgc agttaatgac aaagacttgg ataacaaaac atatgctaaa atcgtagaat
     8341 tgtatcattc aaaagaagct caaaaagcgt tgcaggaaga tgtcaaagat ggagagaaac
     8401 ctgttaattt atctaaagat gagattaagg caatagaaac gtcattagca aaataaatta
     8461 tattgcgtcc tacaagcaaa gttcatgctt atgtttgtag ggcgttattg ttggagaata
     8521 aaattatttc caatagagaa agggattgta atcattttat agtgaaatat tatgaaattg
     8581 taataattta gatattgtaa aatctaataa gttgtaataa ttttaagggg taattataaa
     8641 atttgatgat acagtatatg atttttttgt aatcataatg tcatcaaaca tcaacctatt
     8701 atacataata aaatcgtata atgatgtagt attcataaat tcggataaaa gaatgttagg
     8761 aaagttaagc aagaggagga ttttaaagtg caaaaaaaag taattgcagc tattattggg
     8821 acaagcgcga ttagcgctgt tgcggcaact caagcaaatg cggctacaac tcacacagta
     8881 aaaccgggtg aatcagtgtg ggcaatttca aataagtatg ggatttcgat tgctaaatta
     8941 aagtcattaa acaatttaac atctaatcta attttcccaa accaagtact aaaagtatct
     9001 ggctcaagta attctacgag taatagtagc cgtccatcaa cgaactcagg tggcggatca
     9061 tactacacag tacaagcagg cgactcatta tcattaatcg catcaaaata tggtacaact
     9121 taccaaaaca ttatgcgact taatggttta aataatttct ttatttatcc aggtcaaaaa
     9181 ttaaaagtat caggtactgc tagctcaagt aacgctgcga gcaatagtag ccgtccatca
     9241 acgaactcag gtggcggatc atactataca gtacaagcag gtgactcatt gtcattaatc
     9301 gcatcaaaat atggtacaac ttatcaaaaa attatgagct taaatggctt aaataatttc
     9361 tttatatatc cgggtcaaaa attgaaagta actggtaatg catctacgaa ctcaggatct
     9421 gcaacaacga caaatagagg ttacaataca ccagtattca gtcaccaaaa cttatataca
     9481 tggggtcaat gtacatatca tgtatttaat cgtcgtgctg aaattggtaa aggtattagt
     9541 acttattggt ggaatgctaa taactgggat aacgcagcgg cagcagatgg ttacactatc
     9601 gacaatagac ctactgtagg ttctatcgct caaacagatg taggttatta tggtcatgtt
     9661 atgtttgtag aacgtgtaaa taacgatggt agtattttag tttcagaaat gaactattca
     9721 gctgcaccag gtattttaac ttacagaacg gtaccagctt accaagtaaa taattataga
     9781 tatattcact aaagtcttac gtatataaat atataatgaa ttcctattac atttcacaag
     9841 ctgaaaagtt ggattgaatc ctaaacttta tggcggggct atcaaagtcg tgattgttgt
     9901 aataggaatt tttgtataag aaaaaggatt ggtcgaatga acttcatgtt ctatgttcga
     9961 ccaatccttt tctgaattag tattcatgct ttactttgcg aatgcttgta aataatctag
    10021 caccgtttgt tattaaagta acaactgcca ctgctttttt gaacttacgt ggtgacttaa
    10081 ttgaaatgta aaagtctaac acggttccta cagtgcttaa acctaatgaa agatatacta
    10141 attttttatt tttagcatga tatttatagc cattgtagcc gtcgactatg aaacctgcga
    10201 catttagtaa acttgataaa cgttgtgatt tggaacgttt tgccataata ataattcccc
    10261 ctagtatctt ctgtttatat gaaaaattgg gtgcgctgaa ttgctaacgt tttgcgctat
    10321 aactactcat atatgataac ataattgtac agtataattt taaaaattga tttcacaaag
    10381 ttggggtgtc aaagatgatt aaatgtgtct gtttagttga agaaacagct gataaaatat
    10441 tacttgttca agtaaggaat cgcgaaaagt attatttccc aggtggtaaa atagaagaag
    10501 gggaatcacg agtacaagcg ctgttaagag aagtaaaaga agaattaaat ttaacattaa
    10561 caatggatga aattgaatat atcgggacaa ttgtaggtcc tgcatatcca caacaggata
    10621 tgttaactga gttaaatgga tttcgcgcat taaccaaaat cgattgggaa aacgtaacta
    10681 tcaataatga aattacggat atacgctgga ttgataaaga taatgatgcg ttgattgcgc
    10741 ctgctgtcaa agtttggatt gaaacgtatg gtggtaaaca tgacaaataa tgacaccatc
    10801 atgttacgac attatgtccc acaagattat tcgatgttag aagcttttca attaagtgaa
    10861 agtgatttga agtttgttaa aacgccagag gaaaatatta cagctgcaat gtctgataat
    10921 gaaaggtatc ccatcgttgt aatggatggc aggcaatgtg tggccttttt tacattacat
    10981 cgtggaaaag gggtcgcacc atttagcgat aaccaagatg cagtattttt caggtcattt
    11041 agtgttgatc aacgttatcg taatagagga ataggtaaag tggtaatgga aaaattggcg
    11101 tcatttatca cttcaacatt tcaggatatt aatgagattg tgttaacggt taatactgac
    11161 aatccacatg ccatggcact ttatcgccaa caaggatatc aatatatggg agatagtatg
    11221 ttcatcggaa gacctgttca tattatggcg ttaactataa aataaattaa atttaaaagc
    11281 atctttactc atcgtcgacc acaacaatta atgatgaata aaggtgcttt ttgttataga
    11341 tcatcggaca atttactata gtaaaaagcg acctagtgaa caattgacat atatccacag
    11401 gtcgcttaac ttaagttata ttgctagttg cgattaattg atagactcat catttttgcg
    11461 ctgtcgagat ggtcttttta ttaaaaatgc cgtaatccaa gccgtaatcg gaatactgat
    11521 tgcaacggca ataccgccta aaataataga aataaattct tgggcaaata ttttcgagtt
    11581 tataatatga ccaaatgaat atttaagttt gaaaaaccaa ataaataaag caagttggcc
    11641 accaaaaaag gcaaggtaaa tcgtgttcgc agatgtcgct aaaatttctc taccaacacg
    11701 catgccagat tggaataatt cgtattgcgt aagcgttgga ttcacttgat gcaattcata
    11761 aatgggtgaa ctaatggtaa ttgttaaatc tatcacagct gcaataacag caagaataat
    11821 agtgaacacc ataaattgaa ccatatcaat gccaatattc attgaataca catatgtttc
    11881 atcttgttgt tcggttgaaa agccttgtag atgaccgaag tagaccgata aataaatgag
    11941 tgtaatcaac aatattgttg taacgatagt gctgataaat gcagcttgtg ttttaacatt
    12001 gtaactattg agtacgaata aattacaagc gccaataata atgcagaaaa agaatgtgac
    12061 gacataaatc ggtacgccaa aaataatcaa cacaatacta ataattaaaa tagcaaaatt
    12121 taaaaatagg gttaaataag agatgaatcc ctttctacct ccgaaaatta tcatcagaaa
    12181 gaggagcaat aacgccaata taaatacagc attcattgtt tcgccctcct taatgtttca
    12241 aatatttcca taaacaatat tgtgatagga attgtaagta cgatacctat accacctgtt
    12301 agtgcgcgcg caatttctaa cgaccaattc atcgaaatag tataagtcac tgtattggca
    12361 ttttttaaaa agattaaaaa cataggtagt gcaccggata aatatgagaa taataagatg
    12421 ttagtcattg ttcccataat atcttggccg atgtttcggc cagcaagcgc ccatctcctc
    12481 attgaaatgt gtggcgtacg ctgtaaaatt tcatgcatac cactagcaat tgtaattgca
    12541 acatccataa tagcgccaag tgaacctatt aacactgagg ctaggaagat atctttcggt
    12601 ggtaatgata aaaagttcat cgtttcatat ttaatgcctt taccgtctgt catatatatg
    12661 attaattctg ttaaacctat actcaaaaaa gttccgataa ttgtactggc tatggtaatg
    12721 agtgtgcgca tatgccagcc tgtaacgagc aataaagtga gtattgttga acagatcatg
    12781 gcaatggtca tgagtaagaa taaattaata ttgctatgtt gaatatgaat gtaaattgcg
    12841 attaatatgg caatagaatt caagattaac gataaaatcg attgcagtcc gactttgcga
    12901 ccaaccaata atacagttaa taagaacaaa ccagtgatga taaccgttaa ggtatcacgc
    12961 ttcttttcta taatataagc atcactcggc ttgttagaaa tatgtaataa tactttttcg
    13021 tgtgtgcgaa atgcctcaga atctgcttgc gatttgacgt actgatgatt aatcgtcgtc
    13081 gtttctccag caaattgacc atttaatatt ttgactttta attgattttt atatttaata
    13141 tcacgattat tttgtgcatc ttttgtaggt gtcgaagaaa catgtttgac atctataatt
    13201 tgaccaattg gtttgttgta aaagttctca ttattgaatg taaataaaat agcaccaatg
    13261 aatgcgatgc agaacaaacc taaaattata ttaaatggct ttgtaaataa atttctatat
    13321 ttcaaaaaca aaaccccaat tctatgaatg aattaatatg gtgattatac gcccttaatt
    13381 ttttattttc aaagatatta ctgctaagtg taaaacgaaa atcatcattg atagcatcga
    13441 attacttaat ggaatgtaga cgttttagtc attaattgct gaatgagtgt taataagata
    13501 ccaatatcac tcttcgtata aggttctttc gtaatagcac atatcgttct ttttaattca
    13561 gtatgatcta attttatatc tatccatgat ttagattctg gtaaatgtat attttgtgat
    13621 gaaatgatgt aaccttcttt ttgacgaagg agatactgcg caagtggttg gctactgatt
    13681 gtgtatacat ctgatttagt aatcttgcgc aattgttttt tacagtttcg gcaaatggtg
    13741 ccaagcaata aatatgacta tgctcaaact gaattaatgg tgggtgtgtc gccatcgtaa
    13801 ttggatcgtc tgaaggcgca tataaatgat agtgctcttc gaataaaggt agcatatgta
    13861 attgtttgtg tttacgtatt tctggtgtaa gttccgtgaa accaatgtct atattcccat
    13921 ttaatacgct atttataatc gtgtcatgat ctaataagtt cggtatgaca tgtgtatcat
    13981 tttgtaaatg aaacgtttgg ataagtggta gtaacatgtg ggatacgtca ctctcatcat
    14041 agccaatgta gatactttta tttttagtta atccatggct ttgaaattgt tcaatcgtgc
    14101 tatctaaatg ttcaataatg cgcagagctt cattaaataa taatttccct tcagaagtga
    14161 gcgtaatatt gcgtccttgc tttttaaata aagacacatt aagttcttgt tctaataatg
    14221 taatttgacg gcttatcgct gattgagcaa tgtttagttc aagtgctgtt tcggagatat
    14281 gttctctttt agcgacctcg ataaaatatc ttaattgttt aatttccata gtgatatagg
    14341 cacctccaaa aatgagtgtt ttgtaactat tatagcaata ttattgataa atgttctatt
    14401 ttttagatga atatcttcta ttttatatat tgaacagata aattttttag attatagtaa
    14461 ttatcattaa taactaatat cagaatattc taaaaaaagg ggtgtgcatc atgcacaatg
    14521 agaaattaat taaaggctta tatgactatc gtgaggaaca tgatgcgtgt ggtattggtt
    14581 tttatgcgaa tatggataat aaaaggtctc acgacatcat tgataaatcg cttgaaatgt
    14641 tgcgacgctt agatcacagg ggcggggtcg gcgcagatgg catcactggt gatggcgcag
    14701 gtattatgac tgaaatacct tttgcatttt tcaaacaaca tgtaacggac tttgatatcc
    14761 caggtgaagg tgaatatgcc gtggggttat ttttttccaa agaacgcatt ttaggttctg
    14821 aacatgaagc agtttttaaa aaatattttg aaggtgaagg gttatcaatt cttggttatc
    14881 gtaatgtacc agttaataaa gatgccattg ctaaacatgt agcagatacg atgccagtca
    14941 ttcaacaagt gtttattgat attagggaca ttgaagatgt tgaaaagcgt ttgtttttag
    15001 cgagaaaaca attagagttc tattcgactc agtgcgattt agaattgtat tttacgagct
    15061 tatcacgcaa aacaattgta tataaaggtt ggttacgatc agaccaaatt aaaaaactct
    15121 atacagattt atcggatgat ttatatcaat caaagctagg gttagtgcat tcgagattta
    15181 gtacgaatac attcccgagt tggaaaaggg cgcatcctaa ccgtatgtta atgcataatg
    15241 gtgagattaa cacgattaaa ggtaatgtga actggatgcg agcacgccaa cataaattaa
    15301 tcgaaacatt atttggcgag gatcaacata aagtgtttca aattgtcgat gaggatggta
    15361 gtgactctgc cattgtagat aatgcgctag agttcttatc gttagccatg gagccagaaa
    15421 aggcagcgat gttactcata cctgaacctt ggttatacaa tgaagcgaat gatgcaaatg
    15481 tacgtgcatt ttatgaattt tatagttatt taatggaacc gtgggatggt cctacaatga
    15541 tttcgttctg taacggtgac aaacttggcg cgcttacaga tagaaatgga ttacgtccag
    15601 gtcgttatac gattactaaa gataacttta ttgtcttttc atctgaagtg ggtgttgtgg
    15661 acgtacctga aagtaatgtt gcttttaaag gtcaattgaa tcctggaaag ttattgcttg
    15721 ttgattttaa acagaataaa gtcattgaaa ataatgattt gaaaggtgcg attgctggag
    15781 aattaccata taaagcgtgg attgataacc ataaagttga ctttgatttt gaaaatatac
    15841 aatatcaaga ttcgcaatgg aaagatgaga cgttatttaa attacaacgt cagtttgcat
    15901 acacgaaaga agagattcat aagtatattc aggaacttgt agaaggtaag aaggatccta
    15961 tcggtgcaat gggatatgat gcgccaattg cagtgttgaa cgagcgacca gaatcactat
    16021 ttaattactt taaacagctg tttgcacaag ttacgaatcc accaattgat gcgtatcgtg
    16081 aaaaaatcgt aacgagtgaa ctttcttatt taggtggcga aggtaactta ctagcacctg
    16141 acgaaacggt tttagatcgt attcaattga aaaggccggt attgaatgaa tcacacatag
    16201 cagcgattga tcaggaacat tttaaattaa cttatttatc aacggtatat gaaggggatt
    16261 tggaagatgc gttagaagta ttaggccgag aagcagtgaa tgctgtaaag caaggcgctc
    16321 aaattctagt gttagatgat agtggattag ttgatagcaa tggctttgca atgccgatgt
    16381 tactcgcaat aagtcatgtg catcaattac ttattaaagc agatttacgt atgtctacaa
    16441 gtttagtcgc taaatctggt gagacacgag aagtgcatca tgttgcttgt ttactcgcat
    16501 atggcgcgaa tgcaattgtg ccatacctag cgcaacgtac agttgaacaa ctgacattga
    16561 cagaagggtt acaaggcacc gttgtcgata atgttaagac atatacggat gtattgtccg
    16621 aaggtgtcat taaagtaatg gctaagatgg gaatttcgac agtgcaaagt tatcaaggtg
    16681 cacaaatatt tgaagcgatt ggcttgtctc atgatgtgat tgatcgttat tttactggga
    16741 tacagtctaa gttatcgggt atttcgattg atcaaattga tgctgaaaat aaagcacgtc
    16801 aacaaagtga tgataattat cttgcatcag gtagtacatt ccaatggaga caacaaggtc
    16861 aacatcatgc ttttaatccg gaatctattt tcttattgca gcacgcatgt aaagaaaatg
    16921 actatgcgca atttaaagca tactctgaag cggtgaacaa aaatagaaca gatcacatta
    16981 gacatttact tgaatttaaa gcatgtacac cgattgacat cgaccaagtt gaaccggtaa
    17041 gtgacattgt caaacgcttt aatacagggg cgatgagtta tggatcgatt tcagcggaag
    17101 cacatgaaac gttagcacaa gccatgaacc aattaggtgg aaagagtaat agtggtgaag
    17161 gtggcgaaga tgcaaaacgt tatgaagtac aagttgatgg aagcaacaaa gtaagtgcga
    17221 ttaaacaagt tgcttctggg cgttttggtg taactagtga ttatttacaa catgccaaag
    17281 aaattcaaat taaagttgcg caaggtgcaa agcctggtga aggtggtcaa ttacctggta
    17341 ctaaggtata tccgtggatt gcgaagacaa gagggtcaac gccaggtatc ggtctgattt
    17401 caccaccgcc acatcatgat atttattcaa tagaagattt agcgcaactg atacatgatt
    17461 taaaaaatgc gaataaagat gcagatatcg cggtaaaatt agtttcgaaa acaggtgttg
    17521 gtaccattgc atctggggtg gcaaaagcat ttgcagataa aattgtcatc agtggttacg
    17581 atggtggtac aggggcttca cctaaaacga gtattcagca tgccggtgtt ccttgggaga
    17641 ttggtttagc agaaacacat caaacattaa aactaaacga cttaagaagt cgtgttaagt
    17701 tagaaacaga cggtaagtta ttaactggta aagatgtagc gtacgcatgt gcgcttggag
    17761 cggaagaatt tggatttgca actgcaccat tagtggtgtt gggctgtatt atgatgcgtg
    17821 tatgccataa agatacatgt ccagtaggag ttgcaactca aaacaaagat ttacgtgctt
    17881 tatatagagg taaagcacat catgttgtta attttatgca ttttattgca caagaattaa
    17941 gagaaatttt agcatcttta ggtttgaaac gtgtagaaga cttagttgga agaactgatt
    18001 tattacaacg atcatcaaca ttaaaagtga atagcaaagc ggctagtatt gatgttgaaa
    18061 aactgttatg tcctttcgat gggccaaaca caaaagaaat tcaacaaaat cataatcttg
    18121 agcatggatt tgatttaaca aatttatatg aagtaacgaa gccgtatatt gctgaagggc
    18181 gtcgctatac aggtagcttt acagtaaata atgaacaacg tgatgtagga gttattacag
    18241 gtagtgagat ttcgaaacaa tatggagaag caggacttcc tgaaaataca attaatgttt
    18301 atacgaatgg tcatgctggt caaagtcttg cagcatatgc accgaaaggc ttaatgattc
    18361 atcatactgg agatgcgaat gactatgttg gtaaaggatt atctggtggt acggtcattg
    18421 tcaaagcacc ttttgaagaa cgacaaaatg aaattattgc tggtaacgtc tcattctatg
    18481 gtgcgacagg tggtaaggca tttattaacg gtagtgcagg agaaagattc tgtattagaa
    18541 atagtggtgt agatgttgtc gttgaaggta tcggtgacca tggattagag tacatgactg
    18601 gtggacatgt cattaattta ggtgatgtag gtaagaactt cggtcaaggt atgagtggtg
    18661 gtattgctta cgttatcccg tctgatgtag aagcttttgt tgaaaataat caactagata
    18721 cgctttcgtt tacaaagatt aaacaccaag aagaaaaagc attcattaag caaatgctgg
    18781 aagaacatgt gtcacacacg aatagtacga gagcgattca tgtgttaaaa cattttgatc
    18841 gcattgaaga tgtcgtcgtt aaagttattc ctaaagatta tcaattaatg atgcaaaaaa
    18901 ttcatttgca caaatcatta catgacaatg aagatgaagc gatgttagct gcattttacg
    18961 atgacagtaa aacaatcgac gctaaacata aaccagccgt tgtgtattaa ggaaaggggg
    19021 agatacgatg ggtgaattta aaggatttat gaagtatgac aaacagtact taggtgaatt
    19081 atcactggta gaccgtttga agcatcataa agcatatcaa caacgattta ctaaagaaga
    19141 tgcctctatc caaggtgcac gatgtatgga ttgtggaacg ccgttttgtc aaaccggaca
    19201 acagtatggt agggaaacaa taggttgtcc aattggaaac tacattcctg aatggaacga
    19261 cttagtgtat catcaagatt ttaaaactgc ttatgaacgc ttaagcgaaa caaataactt
    19321 tcctgacttt acagggcgtg tatgtcctgc accatgcgaa agtgcttgtg tgatgaagat
    19381 taatagagaa tcgattgcga ttaaaggtat tgaacgcaca attattgatg aagcttttga
    19441 aaatggttgg gtagcgccga aagttccgag tcgccgtaga gatgaaaaag tggcaatcgt
    19501 tggaagcggt ccagcaggat tagctgctgc tgaagaactt aatctactag gatatcaagt
    19561 aactatttat gaacgtgcta gagaatcagg cggtttatta atgtatggta ttccgaatat
    19621 gaaacttgat aaagatgtgg ttcgacgtcg tattaagtta atggaagaag cgggcattac
    19681 tttcattaat ggtgttgaag taggcgttga tattgataaa gcaacgttag aatctgagta
    19741 tgatgccatt atattatgta ctggtgcaca aaaaggtaga gatttacctt tagaaggacg
    19801 catgggtgat ggtatacatt tcgctatgga ttatttaact gaacaaacgc agttgttaaa
    19861 tggagaaatt gatgatataa caataactgc aaaagataag aatgtcatta tcattggtgc
    19921 tggtgataca ggggcagact gtgtagcgac agcattaaga gaaaattgta aatcgattgt
    19981 tcaatttaat aaatatacga aattgccaga agcaattaca tttacagaaa atgcatcatg
    20041 gcctttagca atgccggtgt ttaaaatgga ctatgcgcac caagagtacg aagctaagtt
    20101 tggtaaggaa ccacgtgcat atggtgttca aacaatgcgt tacgatgttg acgataaagg
    20161 acacatacgt ggtttgtata ctcaaatttt agagcaaggc gaaaatggta tggtcatgaa
    20221 agaaggacct gaaagatttt ggcctgctga ccttgtatta ttatcaatcg gcttcgaagg
    20281 tacagaacca acagtaccga atgcttttaa cattaaaacg gatagaaatc gaatcgtggc
    20341 ggatgataca aactatcaaa ctaataatga aaaggtattt gctgctggag atgctagacg
    20401 tggtcaaagt ttagttgtat gggcgattaa agaaggtaga ggcgtagcga aagcagtaga
    20461 tcagtattta gcgagtaaag tttgtgtata atctttgtat ggaaatggtg gttacgttga
    20521 cgttgtggca tgctgaatcg agtttgaaat aatctagtat ctatcaacgt cacatgccat
    20581 ctttgtaacc taaaaacaaa ggtttgtaag acaacaaatc gattaattat aagtagtgat
    20641 tttttacatt cgtttatagg tcaactgtag tggaagacaa tgatttgtgg taatcatgta
    20701 atgcttaaaa acaatattga cttttacaga acgttcatat atgataaata ttgtgtttag
    20761 gaggaatacc caagtccggc tgaagggatc ggtcttgaaa accgacaggg gcttaacggc
    20821 tcgcgggggt tcgaatccct cttcctccgc catcaatatt tatattaaat actatatata
    20881 atgaaggtaa gtgctcaaat tttgagtatt tgcctttttt atttgtcttt gaatggctcg
    20941 tttttttagc tatagaaatg atagggcatt gagattggaa gggcatttgg cttgtgcaat
    21001 atacatagct aaatgtcttt tttgttttgt gaaatatgat ggatggcttg tgtgggcaag
    21061 tttgctattt atagatatgc atttttcaat ttaggagttg gccatgcatc tacactttat
    21121 aatggtgaga gcgtggtgag gtattgttaa tcacgcaatt gtagcgagga gttattgcta
    21181 catatgtcgt tatggctcat tgattttctg aaatggctac cccagataat tgtgacaaaa
    21241 taaaaaatat ttgttgaaag cctttacata acttgtctag acaagttata ctcgttttaa
    21301 gacattaagg gagtgaaata tatggctgta aaaagagaag atgtaaaagc catcgtaagc
    21361 gctattgggg gaaaagaaaa tcttgaagct gcaacgcatt gtgtaacacg attacgttta
    21421 gtgcttaaag atgaaagcaa agttgataaa gacgcattaa gtaataacgc gttggtcaag
    21481 gggcagttca aagcagacca tcaatatcaa attgtcattg gtccaggaac agtcgatgaa
    21541 gtgtataagc agtttattga tgaaacaggt gctcaagaag cttcgaaaga tgaagcgaaa
    21601 caagcagctg cgcaaaaagg gaatccagta caacgtttga tcaaattgtt aggggatatt
    21661 tttataccaa tattacctgc gattgtgaca gctggtttgt taatggggat caataattta
    21721 cttacaatga aaggtttatt tggtccaaaa gcacttattg agatgtatcc acaaattgct
    21781 gatatttcaa acatcattaa tgtgattgcg agtacggcat ttattttctt accagcatta
    21841 attggttgga gtagtatgcg tgtatttggt ggtagtccga ttctaggcat agtcttaggt
    21901 ttgattttaa tgcatccgca attagtatct cagtatgatt tggcaaaagg gaatattccg
    21961 acgtggaact tatttggctt agagattaag cagttgaatt accaaggtca agtgttgcca
    22021 gttttaattg cagcttatgt tctagctaaa attgaaaaag gattaaataa agtcgttcac
    22081 gattcgataa aaatgttggt cgttggaccc gtagcgcttt tagttactgg atttttagca
    22141 tttattatca ttggaccagt tgcgttattg attggtacag gtattacatc tggtgttaca
    22201 tttatattcc aacatgcagg atggcttggc ggagcaatat atggattgtt atatgcacca
    22261 cttgtaatta caggactaca ccatatgttt ttagcagtag atttccaatt gatgggtagc
    22321 agcttaggtg gtacgtactt atggccaatc gttgcgattt ccaatatttg tcagggctct
    22381 gcagcatttg gagcatggtt tgtctataaa cgtcgtaaaa tggttaaaga agaaggcttg
    22441 gcattaacat cttgtatttc tggtatgtta ggtgttactg aaccagccat gtttggtgtg
    22501 aacttacctc tgaaatatcc atttatcgct gcgatatcaa catcttgtgt attgggggca
    22561 atcgttggta tgaataacgt acttggaaaa gttggtgttg gtggcgtgcc agcattcatt
    22621 tcaattcaaa aagaattttg gccagtatat ctcattgtga cagctattgc tattgttgta
    22681 ccatgtatac taacaattgt gatgtctcat tttagtaaac aaaaagcgaa agaaattgtt
    22741 gaagattaat aaaataaaaa aggggcgttc gttatttgga cgtcctttat tacgttataa
    22801 ggtggtaatt gtgtgtcgaa agaaatagat tggagaaaat ccgttgtata tcaaatttat
    22861 cctaagtcgt ttaatgatac gacggggaat ggtataggag atatcaatgg aattatagaa
    22921 aaattggatt atatcaagtt attgggtgtt gattatattt ggttaacacc agtgtatgaa
    22981 tcaccgatga atgataatgg ctatgatatc agcaattatt tagaaatcaa tgaagacttt
    23041 ggaacgatgg atgattttga aaagttaatc aaagttgctc atcaaaaaga cttgaaagtg
    23101 atgttagata ttgtcattaa tcatacgtcg acggagcatg aatggtttaa agaagcccgt
    23161 aaatctaaag acaaccctta tagagattat tactttttca gatcatctga agacgggccg
    23221 ccaacaaatt ggcattctaa attcggtggt aatgcatgga agtttgattc tgagacagat
    23281 gaatattatt tacatttatt tgatgtcagt caagctgatt taaattggga taatccggaa
    23341 gtacgtcaat cgttatatcg catagtcaat cattggatag acttcggcgt tgatggtttt
    23401 cgatttgatg tcattaactt aatttctaaa ggtgaattta aggactctga caaaataggt
    23461 aaagaatttt atacggatgg tcctagagtg catgagtttc tgcatgaatt aaatcgtcaa
    23521 acgtttggta acactgacat gatgactgta ggagaaatgt cttcgacgac gattgaaaat
    23581 tgtattaagt atacacagcc agaacgccaa gaattgaata gtgtttttaa ttttcatcat
    23641 ctaaaggttg attatgttga tggtgaaaag tggacaaatg cgaagcttga tttccataag
    23701 ttaaagaaaa ttctgatgca atggcaacga ggtatttatg acggtggcgg atggaacgcg
    23761 attttctggt gtaatcatga tcagccacgg gtagtgtcta gatttggtga tgatacgtcg
    23821 gaagagatga ggatacaaag tgctaaaatg ttagctatcg cactgcatat gttgcaaggg
    23881 acgccatata tttaccaagg tgaagaaatt ggtatgacgg acccacattt tacatcaata
    23941 gcacaatatc gcgatgttga atcgattaat gcctaccatc agttgttaag tgaagggcat
    24001 gctgaagcgg atgtgttaac gattttagga cagaagtcac gagacaattc gagaacgcct
    24061 atgcaatgga gtgatgatgt taatgctgga tttacagctg gtaagccttg gattgatatt
    24121 tcggaaaatt atcatcaggt caacgttaga caagcacttc agaataaaga gtctattttc
    24181 tatacgtatc aaaaattaat acaattaaga catacgcatg atattattac gtatggagac
    24241 attgtgccac gttttatgga tcatgatcat ttatttgttt atgaacgtca ttataagaat
    24301 caacaatggc tagtaattgc gaatttctca gcatcggctg ttgatttgcc agaaggattg
    24361 gctagagaag gtcgtgttgt gattcaaaca ggcacagtgg aaaataatac gataagcggg
    24421 tttggtgcaa ttgtaatcga aacaaacgcg taaaataaat tgagtggatg cgtttatatg
    24481 gcgaaacaaa aaaagtttat gaagatttat gaggcgttga aagaagatat attaaacggg
    24541 cagattcaat atggtgaaca aattccgtct gaacatgatt tggtgcaatt gtaccagtca
    24601 tctcgagaga ccgtgcgtaa ggcattagat ttgttggcat tagacggcat gattcaaaag
    24661 attcatggta aagggtcact tgtcatttat caggaggtta cagagtttcc attttctgaa
    24721 ctggttagtt ttaaagaaat gcaagaagaa atgggcgtcg catatttaac tgaagttgtt
    24781 gtgaatgagg ttgttgaagc gcatgaagtt ccagaagttc aacatgcttt aaacatcaat
    24841 tctagtgaat cactcattca tattgttaga actcgtcggc ttaaccaaca tgtgaagatt
    24901 gttgatgaag attattttct aaagtcgatt gtttcagaca taggtaatga tgttgcgagt
    24961 gattctattt atgattattt ggaaaaggta ttaaatctta atattagtta ttcaagtaag
    25021 tctattactt ttgaaccgtt tgatgaacaa gcatatcaat tgtttggtga tgtatcggtg
    25081 gcttattcag caacagttcg aagtattgtg tatttagaaa atacaatgcc gtttcaatat
    25141 aatatttcaa aacatcttgc aaatgaattt aaatttaacg acttctcaag acgtcgtata
    25201 aagtaaacaa tgatataaat gatttatact tgcaattaac tattaaaata tagtaatata
    25261 tatcttgccg tgctaggtgg ggaggtagcg gttccctgta ctcgaaatcc gctttatgcg
    25321 aggcttaatt cctttgttga ggccgtattt ttgcgaagtc tgcccaaagc acgtagtgtt
    25381 tgaagatttc ggtcctatgc aatatgaacc catgaaccat gtcaggtcct gacggaagca
    25441 gcattaagtg gatcatcata tgtgccgtag ggtagccgag atttagctaa cgactttggt
    25501 tacgttcgtg aattacgttc gatgcttagg tgcacggttt tttatttttt aaatattaaa
    25561 ccgattatta agagttgaaa atatatattt atttatagaa gctactttct tgaagacaat
    25621 tcagcgtatt atacgtggaa catgtttgtg ggaagtagct tttttatatg tgaagtttga
    25681 ttcaagtgaa atcgatgtgc agtttgaatg atttttgtgt caatgaaaag taagaagtta
    25741 ttatttgatg ataaagaaat gatggtgaaa tgagggggag tatcttacaa tagaattatt
    25801 aatgagatac gttatgatta ttgacaatca aatgcctacg gaggacatat gcaaatatat
    25861 ttaagtactt taacagagtt agattatgat aaatctttaa atagtattga agaaagtttt
    25921 gatgataatc ctgaaacgag ttggcaagca cttgcgaaag taaaacattt aagaaaatct
    25981 ccttgctata attttgaatt agaagtaata gcgaaaaatg aaaataacga tgtcgttgga
    26041 cacgttttat taattgaagt agaaattaat agtgatgata agacgtatta tggtttggcg
    26101 attgcctctt tatcagttca tcctgaatta cgtggacaaa aattaggtcg tggcttggtt
    26161 caagcagtag aagagcgtgc caaagcacaa gagtatagta cggttgttgt agaccattgt
    26221 tttgactact ttgaaaagtt gggttatcaa aatgctgctg agcatgacat taaattagaa
    26281 tctggtgatg caccgttact tgtaaaatat ttatgggata atttgacgga tgcaccacac
    26341 ggaatcgtaa aatttccaga acatttttat taattgttca attaagaagt aaaggtatta
    26401 tcatgctata atgagaggta attgtttatg gaggtgctaa cttgaattat caagccttat
    26461 atcgtatgta cagaccccaa agtttcgagg atgtcgtcgg acaagaacat gtcacgaaga
    26521 cattgcgcaa tgcgatttct aaagaaaaac agtcgcatgc ttatattttt agtggtccga
    26581 gaggcacggg gaaaacgagt attgccaaag tgtttgctaa agcaatcaac tgtttaaata
    26641 gcactgatgg agaaccttgt aatgaatgtc atatttgtaa aggcattacg caggggacta
    26701 attcagatgt gatagaaatt gatgctgcta gtaataatgg cgttgatgaa ataagaaata
    26761 ttagagacaa agttaaatat gcaccaagtg aattgaaata taaagtttat attatagatg
    26821 aggtgcacat gctaacaaca ggtgctttta atgccctttt aaagacgtta gaagaacctc
    26881 cagcacacgc tatttttata ttggcaacga cagaaccaca taaaatccct ccaacaatca
    26941 tttctagggc acaacgtttt gattttaaag caattagcct aaatcaaatt gttgaacgtt
    27001 taaaatttgt agcagatgca caacaaattg aatgtgaaga tgaagccttg gcatttatcg
    27061 ctaaagcgtc tgaagggggt atgcgtgatg cattaagtat tatggatcag gctattgcat
    27121 ttggtgatgg tacgttaaca ttgcaagatg cgttgaatgt cacaggtagc gtacatgatg
    27181 aagcgttgga tcacttgttt gatgatattg tacaaggtga cgtacaagca tcttttaaaa
    27241 aataccatca gtttataaca gagggtaaag aagtgaatcg cctaataaat gatatgattt
    27301 attttgtcag agatacgatt atgaataaaa catctgagaa agatactgag tatcgagcac
    27361 tgatgaactt agaattagat atgttatatc aaatgattga tcttattaat gatacattag
    27421 tgtcgattcg ttttagtgtg aatcaaaacg ttcattttga agtgttgtta gtaaaattag
    27481 ctgagcagat taagggtcaa ccacaagtga ttgcgaatgt agctgaacca gcacaaattg
    27541 cttcatcgcc aaacacagat gtattgttgc aacgtatgga acagttagag caagaactaa
    27601 aaacactaaa agcacaagga gtgagtgtcg ctcctgttca aaaatcttcg aaaaagcctg
    27661 cgagaggcat acaaaaatct aaaaatgcat tttcaatgca acaaattgca aaagtgctag
    27721 ataaagcgaa taaggcagat atcaaattgt tgaaagatca ttggcaagaa gtgattgatc
    27781 atgccaaaaa caatgataaa aaatcactcg ttagtttatt gcaaaattcg gaacctgtgg
    27841 cggcaagtga agatcacgta cttgtgaaat ttgaggaaga gatccattgt gaaatcgtca
    27901 ataaagacga cgagaaacgt agtagtatag aaagtgttgt atgtaatatc gttaataaaa
    27961 acgttaaagt tgttggtgta ccatcagatc aatggcaaag agttcgaacg gagtatttac
    28021 aaaatcgtaa aaacgaaggc gatgatatgc caaagcaaca agcacaacaa acagatattg
    28081 ctcaaaaagc aaaagatctt ttcggtgaag aaactgtaca tgtgatagat gaagagtgat
    28141 acatgacaag cgatataatc gtatgtataa tgaaagaaac atcattttat tgataaatat
    28201 ttattgattt tcaaggagga aatggaatat gcgcggtggc ggaaacatgc aacaaatgat
    28261 gaaacaaatg caaaaaatgc aaaagaaaat ggctcaagaa caagaaaaac ttaaagaaga
    28321 gcgtattgta ggaacagctg gcggtggcat ggttgcagtt actgtaactg gtcataaaga
    28381 agttgtcgac gttgaaatca aagaagaagc tgtagaccca gatgatattg aaatgctaca
    28441 agacttagtg ttagcagcta ctaatgaagc gatgaataaa gctgatgagc ttactcaaga
    28501 acgtttaggt aaacatactc aaggcttaaa catccctgga atgtgatcat agatgcatta
    28561 tccagaacct atatcaaaac ttattgatag ctttatgaaa ttgccaggca ttggtccaaa
    28621 gacagcccaa cgtctggctt ttcatacctt agatatgaaa gaagacgatg ttgttcagtt
    28681 tgccaaagca ttagtagatg ttaagagaga attaacatat tgtagcgtat gtggtcacat
    28741 tactgaaaat gatccatgtt atatttgtga agataagcaa agagatcgtt cagttatttg
    28801 tgttgtggaa gatgacaaag atgtcatagc tatggaaaaa atgagagaat acaaaggttt
    28861 atatcacgtt ttacatgggt ctatttcgcc tatggatggc attggaccag aagatattaa
    28921 tattccttca ttgattgaac gcttgaaaaa cgatgaagtt agcgaattaa tcttagctat
    28981 gaacccgaac ttagaggggg aatctacagc catgtatatt tctagattag ttaagcctat
    29041 aggtatcaaa gtgacgagat tagcacaagg gttatcggta ggtggcgatt tagagtatgc
    29101 tgacgaagta acattatcta aagcaatcgc aggtagaaca gaaatgtaat gtcttctatt
    29161 aaacattttt gattttaata ctatagtaag aaaagtcaca gtgtaatcat tgtggctttt
    29221 tttatggtgt ggtgtgatgt actactttat ttgcggtgtg gcggtggtat ggtttaccta
    29281 gttttactga gggatgggta atctttagga agcaagccgt tggttgtgat ttgttacttc
    29341 taatagtaat gatgtgaatt ggattatcga attagatcta tggttatggt gtgttggtgc
    29401 tattaatttg ataaatgcgg ttaatgacta tgcaaatgaa attcttttgt aattgaaatg
    29461 atagatgctg gcttagtaag ttgtacttct ttggtctaaa gcttattaaa tcagcctgta
    29521 tagcggtgtt ttgagagatt atttaaaact tgtaaattta tttttaattt ctggtaaaaa
    29581 aataacgttc tgttttgcgg tttttttgat tgatatggtt agaaaaaaat ctgtttcttg
    29641 ttctaaaaaa cgtactattt ataagtgggg atttttttaa gttcgatttt taggataagg
    29701 gcgttcagta cagatgacaa aggtgtaatt tttactgttg ttaagcagtt tgaaagcctg
    29761 tatagtattt atttgttgag gcaaacaaaa caattcaact taagaaataa cttgaattac
    29821 taacgaaaat taatt
//
LOCUS       DAMZKC010000015        29120 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.015, whole genome shotgun sequence.
ACCESSION   DAMZKC010000015 DAMZKC010000000
VERSION     DAMZKC010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 29120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29120
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.015"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            132..2207
                     /gene="topA"
                     /locus_tag="POB55_000481"
     CDS             132..2207
                     /gene="topA"
                     /locus_tag="POB55_000481"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HDI6499473.1"
                     /translation="MTLADNLVIVESPAKAKTIEKYLGKKYKVIASMGHVRDLPRSQM
                     GVDTEDNYEPKYITIRGKGPVVKELKKHAKKAKNVFLASDPDREGEAIAWHLSKILEL
                     EDSKENRVVFNEITKDAVKESFKNPREIEMNLVDAQQARRILDRLVGYNISPVLWKKV
                     KKGLSAGRVQSVALRLVIDRENEIRNFKPEEYWTIEGEFRYKKSKFNAKFLHYKNKPF
                     KLKTKKDVEKITAALDGDQFEITNVTKKEKTRNPANPFTTSTLQQEAARKLNFKARKT
                     MMVAQQLYEGIDLKKQGTIGLITYMRTDSIRISDTAKAEAKQYITDKYGESYTSKRKA
                     SGKQGDQDAHEAIRPSSTMRTPDDMKSFLTKDQYRLYKLIWERFVASQMAPAILDTVS
                     LDITQGDIKFRANGQTIKFKGFMTLYVETKDDSDSEKENKLPKLEQGDKVTATQIEPA
                     QHYTQPPPRYTEARLVKTLEELKIGRPSTYAPTIDTIQKRNYVKLESKRFVPTELGEI
                     VHEQVKEYFPEIIDVEFTVNMETLLDKIAEGDITWRKVIDGFFSSFKQDVERAEEEME
                     KIEIKDEPAGEDCEVCGSPMVIKMGRYGKFMACSNFPDCRNTKAIVKSIGVKCPKCND
                     GDVVERKSKKNRVFYGCSKYPECDFISWDKPIGRDCPKCNQYLVENKKGKTTQVICSN
                     CDYKEAAQK"
     gene            2363..3670
                     /gene="trmFO"
                     /locus_tag="POB55_000482"
     CDS             2363..3670
                     /gene="trmFO"
                     /locus_tag="POB55_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HDI6499474.1"
                     /translation="MTQTVNVIGAGLAGSEAAYQLAERGIKVNLIEMRPVKQTPAHHT
                     DKFAELVCSNSLRGNALTNGVGVLKEEMRRLNSIIIEAADKARVPAGGALAVDRHDFS
                     GYITETLKNHENITVINEEINAIPDGYTIIATGPLTTETLAQEIVDITGKDQLYFYDA
                     AAPIIEKESIDMDKVYLKSRYDKGEAAYLNCPMTEDEFNRFYDAVLEAEVAPVNSFEK
                     EKYFEGCMPFEVMAERGRKTLLFGPMKPVGLEDPKTGKRPYAVVQLRQDDAAGTLYNI
                     VGFQTHLKWGAQKEVIKLIPGLENVDIVRYGVMHRNTFINSPDVLNEKYELISQPNIQ
                     FAGQMTGVEGYVESAASGLVAGINLAHKILGKGEVVFPRETMIGSMAYYISHAKNNKN
                     FQPMNANFGLLPSLETRIKDKKERYEAQANRALDYLENFKKTL"
     gene            4087..4983
                     /gene="xerC"
                     /locus_tag="POB55_000483"
     CDS             4087..4983
                     /gene="xerC"
                     /locus_tag="POB55_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HDI6499475.1"
                     /translation="MNHIQEAFLNTLKVERNFSEHTLKSYQDDLIQFNQFLEQEHLQL
                     KTFEYRDARNYLSYLYSNHLKRTSVSRKISTLRTFYEYWMTLDENIINPFVQLVHPKK
                     EKYLPQFFYEEEMEALFKTVEEDTSKSLRDRVILELLYATGIRVSELVNIKKQDIDFY
                     ANGVTVLGKGSKERFVPFGAYCRQSIENYLEHFKPIQSCNHDFLIVNMKGEAITERGV
                     RYVLNDIVKRTAGVSEIHPHKLRHTFATHLLNQGADLRTVQSLLGHVNLSTTGKYTHV
                     SNQQLRKVYLNAHPRAKKENET"
     gene            4980..5525
                     /gene="hslV"
                     /locus_tag="POB55_000484"
     CDS             4980..5525
                     /gene="hslV"
                     /locus_tag="POB55_000484"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HDI6499476.1"
                     /translation="MSNTTLHATTIYAVRHNGKAAMAGDGQVTLGQQVIMKQTARKVR
                     RLYEGKVLAGFAGSVADAFTLFEKFETKLQQFSGNLERAAVELAQEWRGDKQLRQLEA
                     MLIVMDKDAILVVSGTGEVIAPDDDLIAIGSGGNYALSAGRALKRHASHLSAEEMAYE
                     SLKVAADICVFTNDNIVVETL"
     gene            5591..6994
                     /gene="hslU"
                     /locus_tag="POB55_000485"
     CDS             5591..6994
                     /gene="hslU"
                     /locus_tag="POB55_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HDI6499477.1"
                     /translation="MDTAGIRLTPKEIVSKLNEYIVGQNDAKRKVAIALRNRYRRSLL
                     DEESKQEISPKNILMIGPTGVGKTEIARRMAKVVGAPFIKVEATKFTEVGYVGRDVES
                     MVRDLVDVSVRLVKAQKKSLVQDEATAKANEKLVKLLVPSMKKKASQTNNPLESLFGG
                     AIPNFGQNNEDEEEPPTEEIKTKRSEIKRQLEEGKLEKEKVRIKVEQDPGALGMLGTN
                     QNQQMQEMMNQLMPKKKVEREVAVETARKILADSYADELIDQESANQEALELAEQMGI
                     IFIDEIDKVATNNHNSGQDVSRQGVQRDILPILEGSVIQTKYGTVNTEHMLFIGAGAF
                     HVSKPSDLIPELQGRFPIRVELDSLSVEDFVRILTEPKLSLIKQYEALLQTEEVTVNF
                     TDEAITRLAEIAYQVNQDTDNIGARRLHTILEKMLEDLSFEAPSMPNAVVDITPQYVD
                     DKLKSISTNKDLSAFIL"
     gene            7019..7792
                     /gene="codY"
                     /locus_tag="POB55_000486"
     CDS             7019..7792
                     /gene="codY"
                     /locus_tag="POB55_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HDI6499478.1"
                     /translation="MSLLSKTRELNTLLQKHKGIAVDFKDVAQTISSVTVTNVFIVSR
                     RGKILGSSLNELLKSQRIIQMLEERHIPSEYTERLMEVKQTESNIDIDNVLTVFPPEN
                     RELFIDSRTTIFPILGGGERLGTLVLGRVHDDFNENDLVLGEYAATVIGMEILREKHS
                     EVEKEARDKAAITMAINSLSYSEKEAIEHIFEELGGTEGLLIASKVADRVGITRSVIV
                     NALRKLESAGVIESRSLGMKGTFIKVKKEKFLDELEKSK"
     gene            7843..7935
                     /locus_tag="POB55_000487"
     CDS             7843..7935
                     /locus_tag="POB55_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499479.1"
                     /translation="MAVVNEMTLMIMLKSFLYFCLIFNQIDIPH"
     gene            8134..8901
                     /gene="rpsB"
                     /locus_tag="POB55_000488"
     CDS             8134..8901
                     /gene="rpsB"
                     /locus_tag="POB55_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HDI6499480.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKKVDEAYNFLKQVSEDGGQVLFVGTKKQAQESVKSEAERAGQFYINQRWLGGLLT
                     NYKTISKRIKRISEIEKMEEDGLFEVLPKKEVVELKKEYDRLIKFLGGIRDMKSMPQA
                     LFVVDPRKERNAIAEARKLNIPIVGIVDTNCDPDEIDYVIPANDDAIRAVKLLTAKMA
                     DAILEGQQGVSNEEVAAEQNIDLDEKEKSEETEATEE"
     gene            8935..9048
                     /locus_tag="POB55_000489"
     CDS             8935..9048
                     /locus_tag="POB55_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499481.1"
                     /translation="MGDKILMLITFFKKKIEANYKYSIRVLAILPIIMLKS"
     gene            9083..9964
                     /locus_tag="POB55_000490"
     CDS             9083..9964
                     /locus_tag="POB55_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HDI6499482.1"
                     /translation="MATISAKLVKELREKTGAGMMDCKKALTETDGDIDKAIDYLREK
                     GIAKAAKKADRIAAEGLVHVETKGNDAVIVEINSETDFVARNEGFQELVKEIANQVLD
                     TKAETVEALMETTLPNGKSVDERIKEAISTIGEKLSVRRFAIRTKTDNDAFGAYLHMG
                     GRIGVLTVVEGSTDEEAARDVAMHIAAINPKYVSSEQVSEEEINHEREVLKQQALNEG
                     KPENIVEKMVEGRLRKYLQEICAVDQDFVKNPDVTVEAFLKTKGGKLVDFVRYEVGEG
                     MEKREENFADEVKGQMK"
     gene            10101..10823
                     /locus_tag="POB55_000491"
     CDS             10101..10823
                     /locus_tag="POB55_000491"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HDI6499483.1"
                     /translation="MAQISKYKRVVLKLSGEALAGEKGFGINPVIIKSVAEQVAEVAK
                     MDCEIAVIVGGGNIWRGKTGSDLGMDRGTADYMGMLATVMNALALQDSLEQLDCDTRV
                     LTSIEMKQVAEPYIRRRAIRHLEKKRVVIFAAGIGNPYFSTDTTAALRAAEVEADVIL
                     MGKNNVDGVYSADPKVNKDAVKYEHLTHIQMLQEGLQVMDSTASSFCMDNNIPLTVFS
                     IMEEGNIKRAVMGEKIGTLITK"
     gene            10842..11396
                     /gene="frr"
                     /locus_tag="POB55_000492"
     CDS             10842..11396
                     /gene="frr"
                     /locus_tag="POB55_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HDI6499484.1"
                     /translation="MSDIINETKSRMQKSIESLSRELANISAGRANSNLLNGVTVDYY
                     GAPTPVQQLASINVPEARLLVISPYDKTSVADIEKAIIAANLGVNPTSDGEVIRIAVP
                     ALTEERRKERVKDVKKIGEEAKVSVRNIRRDMNDQLKKDEKNGDITEDELRSGTEDVQ
                     KATDNSIKEIDQMIADKEKDIMSV"
     gene            11769..12539
                     /locus_tag="POB55_000493"
     CDS             11769..12539
                     /locus_tag="POB55_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HDI6499485.1"
                     /translation="MFKKLINKKNTINNYNEELDSSNIPEHIAIIMDGNGRWAKKRKM
                     PRIKGHYEGMQTIKKITRVASDIGVKYLTLYAFSTENWSRPESEVNYIMNLPVNFLKT
                     FLPELIEKNVKVETIGFTDKLPKSTIEAINNAKEKTANNTGLKLIFAINYGGRAELVH
                     SIKNMFDELHQQGLNSDIIDETYINNHLMTKDYPDPELLIRTSGEQRISNFLIWQVSY
                     SEFIFNQKLWPDFDEDELIKCIKIYQSRQRRFGGLSEE"
     gene            12546..13328
                     /locus_tag="POB55_000494"
     CDS             12546..13328
                     /locus_tag="POB55_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDI6499486.1"
                     /translation="MKVRTLTAIIALIVFLPILLKGGLVLMIFANILALIALKELLNM
                     NMIKFVSVPGLISAVGLIIIMLPQHAGPWVQVIQLKSLIAMSFIVLSYTVLSKNRFSF
                     MDAAFCLMSVAYVGIGFMFFYETRSEGLHYILYAFLIVWLTDTGAYLFGKMMGKHKLW
                     PVISPNKTIEGFIGGLFCSLIVPLAMLYFVDFNMNVWILLGVTLILSLFGQLGDLVES
                     GFKRHFGVKDSGRILPGHGGILDRFDSFMFVLPLLNILLIQS"
     gene            13540..14826
                     /gene="rseP"
                     /locus_tag="POB55_000495"
     CDS             13540..14826
                     /gene="rseP"
                     /locus_tag="POB55_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HDI6499487.1"
                     /translation="MSYLVTIIAFIIVFGVLVTVHEYGHMFFAKRAGIMCPEFAIGMG
                     PKIFSFRKNETLYTIRLLPVGGYVRMAGDGLEEPPVEPGMNVKIKLNEENEITHIILD
                     DHHKFQQIEAIEVKKCDFKDDLFIEGITAYDNERHHFKIARKSFFVENGSLVQIAPRD
                     RQFAHKKPWPKFLTLFAGPLFNFILALVLFIGLAYYQGTPTSTVEQVADKYPAQQAGL
                     QKGDKIVQIGKYKISEFDDVDKALDKVKDNKTTVKFERDGKTKSVELTPKKTERKLTK
                     VSSETKYVLGFQPASERTLFKPIVYGFESFLKGSTLIFTAVVGMLASIFTGGFSFDML
                     NGPVGIYHNVDSVVKAGIISLIGYTALLSVNLGIMNLIPIPALDGGRILFVIYEAIFR
                     KPVNKKAETTIIAIGAIFMVVIMILVTWNDIRRYFL"
     gene            14846..16549
                     /locus_tag="POB55_000496"
     CDS             14846..16549
                     /locus_tag="POB55_000496"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDI6499488.1"
                     /translation="MKQSKVFIPTMRDVPSEAEAQSHRLLLKSGLIKQSTSGIYSYLP
                     LATRVLNNITAIVRQEMERIDSVEILMPALQQAELWEESGRWGAYGPELMRLQDRHGR
                     QFALGPTHEELVTSIVRNELKSYKQLPMTLFQIQSKFRDEKRPRFGLLRGREFIMKDA
                     YSFHADEASLDQTYQDMYQAYSRIFERVGINARPVVADSGAIGGSHTHEFMALSAIGE
                     DTIVYSKESDYAANIEKAEVVYEPNHKYSTVQPLEKIETPNVKTAQELADFLGRPVDE
                     IVKTMIFKVDGEYIMVLVRGHHEINDIKLKSYFGTDNIELATQDEIVNLVGANPGSLG
                     PVIDKEIKIYADNFVQDLNNLVVGANEDGYHLINVNVGRDFNVDEYGDFRFILEGEKL
                     SDGSGVAHFAEGIEVGQVFKLGTKYSESMNATFLDNQGKAQPLIMGCYGIGISRTLSA
                     IVEQNHDDNGIVWPKSVTPFDLHLISINPKKDDQRELADALYAEFNTKFDVLYDDRQE
                     RAGVKFNDADLIGLPLRIVVGKRASEGIVEVKERLTGDSEEVHIDDLMTVITNKYDNL
                     K"
     gene            16813..21123
                     /locus_tag="POB55_000497"
     CDS             16813..21123
                     /locus_tag="POB55_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDI6499489.1"
                     /translation="MTEQQKFKVLADQIKISNQLDAEILNSGELTRIDVSNKNRTWEF
                     HITLPQFLAHEDYLLFINAIEQEFKDIANVTCRFTVTNGTNQDEHAIKYFGHCIDQTA
                     LSPKVKGQLKQKKLIMSGKVLKVMVSNDIERNHFDKACNGSLIKAFRNCGFDIDKIIF
                     ETNDNDQEQNLASLEAHIQEEDEQSARLATEKLEKMKAEKAKQQDNNESAVDKCQIGK
                     PIQIENIKPIESIIEEEFKVAIEGVIFDINLKELKSGRHIVEIKVTDYTDSLVLKMFT
                     RKNKDDLEHFKALSVGKWVRAQGRIEEDTFIRDLVMMMSDIEEIKKATKKDKAEEKRV
                     EFHLHTAMSQMDGIPNIGAYVKQAADWGHPAIAVTDHNVVQAFPDAHAAAEKHGIKMI
                     YGMEGMLVDDGVPIAYKPQDVVLKDATYVVFDVETTGLSNQYDKIIELAAVKVHNGEI
                     IDKFERFSNPHERLSETIINLTHITDDMLVDAPEIEEVLTEFKEWVGDAIFVAHNASF
                     DMGFIDTGYERLGFGPSTNGVIDTLELSRTINTEYGKHGLNFLAKKYGVELTQHHRAI
                     YDTEATAYIFIKMVQQMKELGVLNHNEINKKLSNEDAYKRARPSHVTLIVQNQQGLKN
                     LFKIVSASLVKYFYRTPRIPRSLLDEYREGLLVGTACDEGELFTAVMQKDQSQVEKIA
                     KYYDFIEIQPPALYQDLIDRELIRDTETLHEIYQRLIHAGDTAGIPVIATGNAHYLFE
                     HDGIARKILIASQPGNPLNRSTLPEAHFRTTDEMLNEFHFLGEEKAHEIVVKNTNELA
                     DRIERVVPIKDELYTPRMEGANEEIRELSYANARKLYGEDLPQIVIDRLEKELKSIIG
                     NGFAVIYLISQRLVKKSLDDGYLVGSRGSVGSSFVATMTEITEVNPLPPHYICPNCKT
                     SEFFNDGSVGSGFDLPDKTCETCGAPLIKEGQDIPFETFLGFKGDKVPDIDLNFSGEY
                     QPNAHNYTKVLFGEDKVFRAGTIGTVAEKTAFGYVKGYLNDQGIHKRGAEIDRLVKGC
                     TGVKRTTGQHPGGIIVVPDYMDIYDFTPIQYPADDQNSAWMTTHFDFHSIHDNVLKLD
                     ILGHDDPTMIRMLQDLSGIDPKTIPVDDKEVMQIFSTPESLGVTEDEILCKTGTFGVP
                     EFGTGFVRQMLEDTKPTTFSELVQISGLSHGTDVWLGNAQELIKTGICDLSSVIGCRD
                     DIMVYLMYAGLEPSMAFKIMESVRKGKGLTEEMIETMKENEVPDWYLDSCLKIKYMFP
                     KAHAAAYVLMAVRIAYFKVHHPLYYYASYFTIRASDFDLITMIKDKTSIRNTVKDMYS
                     RYMDLGKKEKDVLTVLEIMNEMAHRGYRMQPISLEKSQAFEFIIEGDTLIPPFISVPG
                     LGENVAKRIVEARDDGPFLSKEDLNKKAGLSQKIIEYLDELGSLPNLPDKAQLSIFDM
                     "
     gene            21413..21880
                     /gene="rimP"
                     /locus_tag="POB55_000498"
     CDS             21413..21880
                     /gene="rimP"
                     /locus_tag="POB55_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HDI6499490.1"
                     /translation="MSKITEQVEVIVKPIMEDLNFELVDVEYVKEGRDHFLRISIDKE
                     GGVDLNDCTLASEKISEAMDANDPIPEMYYLDVASPGAERPIKKEQDFQNAITKPVFV
                     SLYVPIEGEKEWLGILQEVNNETIVVQVKIKARTKDIEIPRDKIAKARHAVMI"
     gene            21901..23076
                     /gene="nusA"
                     /locus_tag="POB55_000499"
     CDS             21901..23076
                     /gene="nusA"
                     /locus_tag="POB55_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HDI6499491.1"
                     /translation="MSSNELLLATEYLEKEKKIPRAVLIDAIEAALITAYKKNYDSAR
                     NVRVELNMDQGTFKVIARKDVVEEVFDDRDEVDLSTALVKNPAYEIGDIYEEDVTPKD
                     FGRVGAQAAKQAVMQRLRDAEREILFEEFIDKEEDILTGIIDRVDHRYVYVNLGRIEA
                     VLSEAERSPNEKYIPNERIKVYVNKVEQTTKGPQIYVSRSHPGLLKRLFEQEVPEIYD
                     GTVIVKSVAREAGDRSKISVFSENNDIDAVGACVGAKGARVEAVVEELGGEKIDIVQW
                     NEDPKVFVKNALSPSQVLEVIVDETNQSTIVVVPDYQLSLAIGKRGQNARLAAKLTGW
                     KIDIKSETDAREAGIYPVVEAEKVTEEDVALEDADTTESTEEVNDVSVETNVEKESE"
     gene            23097..23381
                     /locus_tag="POB55_000500"
     CDS             23097..23381
                     /locus_tag="POB55_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HDI6499492.1"
                     /translation="MKKKKIPMRKCILSNEMHPKKDMIRVVVNKEGEIFADVTGKKQG
                     RGAYVSKDVAMVEKAQQKEILEKYFKASKEQLDPVYKEIIRLIYREEIPK"
     gene            23378..23695
                     /locus_tag="POB55_000501"
     CDS             23378..23695
                     /locus_tag="POB55_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ family RNA-binding protein"
                     /protein_id="HDI6499493.1"
                     /translation="MSIDQILNFLGLAMRAGKVKTGESVIVNEIKKGNLKLVIVANDA
                     SDNTAKLITDKCKSYKVPFRKFGNRNELGIALGKGERVNVGITDPGFAKKLLSMIDEY
                     HKE"
     gene            23700..25817
                     /gene="infB"
                     /locus_tag="POB55_000502"
     CDS             23700..25817
                     /gene="infB"
                     /locus_tag="POB55_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDI6499494.1"
                     /translation="MSKQRIYEYAKELNLKSKEIIDELKSMNIEVSNHMQALEDDQIK
                     ALDKKFKKEQKNDNKQSTQNNHQKSNNQNQNKGQQKDNKKNQQQNNKGNKGNKKNNRN
                     NKKNNKNNKPQNQPAAPKEIPSKVTYQEGITVGEFADKLNVESSEIIKKLFLLGIVAN
                     INQSLNQETIELIADDYGVEVEEEVVINEEDLSIYFEDEKDDPEAIERPAVVTIMGHV
                     DHGKTTLLDSIRHTKVTAGEAGGITQHIGAYQIENDGKKITFLDTPGHAAFTTMRARG
                     AQVTDITILVVAADDGVMPQTIEAINHAKEAEVPIIVAVNKIDKPTSNPDRVMQELTE
                     YGLIPEDWGGETIFVPLSALSGDGIDDLLEMIGLVAEVQELKANPKNRAVGTVIEAEL
                     DKSRGPSASLLVQNGTLNVGDAIVVGNTYGRIRAMVNDLGQRIKMAGPSTPVEITGIN
                     DVPQAGDRFVVFSDEKQARRIGESRHEASIVQQRQESKNVSLDNLFEQMKQGEMKDLN
                     VIIKGDVQGSVEALAASLMKIDVEGVNVRIIHTAVGAINESDVTLANASNGIIIGFNV
                     RPDSGAKRAAEAENVDMRLHRVIYNVIEEIESAMKGLLDPEFEEQVIGQAEVRQTFKV
                     SKVGTIAGCYVTEGKITRNAGVRIIRDGIVQYEGELDTLKRFKDDAKEVAKGYECGIT
                     IENYNDLKEGDVIEAFEMVEIKR"
     gene            26203..26553
                     /gene="rbfA"
                     /locus_tag="POB55_000503"
     CDS             26203..26553
                     /gene="rbfA"
                     /locus_tag="POB55_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HDI6499495.1"
                     /translation="MSSMRAERVGEQMKKELMDIINNKVKDPRVGFITITDVVLTNDL
                     SQAKVFLTVLGNDKEVENTFKALDKAKGFIKSELGSRMRLRIMPELMYEYDQSIEYGN
                     KIERMIQDLHKQDR"
     gene            26723..27640
                     /gene="truB"
                     /locus_tag="POB55_000504"
     CDS             26723..27640
                     /gene="truB"
                     /locus_tag="POB55_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HDI6499496.1"
                     /translation="MYNGILPVYKERGLTSHDVVFKLRKILKTKKIGHTGTLDPEVAG
                     VLPVCIGNATRVSDYVMDMGKAYEATVSIGRSTTTEDQTGDTLEKKGVHSADFNKDDI
                     DRLLESFKGIIEQIPPMYSSVKVNGKKLYEYARNNETVERPKRKVNIKDIGRISELDF
                     KENECHFKIRVICGKGTYIRTLATDIGVKLGFPAHMSKLTRIESGGFVLKDSLTLEQI
                     KELHEQDSLQNKLFPLEYGLKGLPSIKIKDSHIKKRILNGQKFNKNEFDNKIKDQIVF
                     IDDDSEKVLAIYMVHPTKESEIKPKKVFN"
     gene            27655..28626
                     /gene="ribF"
                     /locus_tag="POB55_000505"
     CDS             27655..28626
                     /gene="ribF"
                     /locus_tag="POB55_000505"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HDI6499497.1"
                     /translation="MKVIEVTHPIQSKQYNTEDVAMAFGFFDGMHKGHDKVFDILNEI
                     AEARSLKKAVMTFDPHPSVVLNPKRKRTTYLTPLSDKIEKISQHDIDYCIVVNFSSRF
                     ANVSVEDFVENYIIKNNVKEVIAGFDFTFGKFGKGNMTVLQEYDAFNTTIVSKQEIEN
                     EKISTTSIRQDLINGELQKANDALGYIYSIKGTVVQGEKRGRTIGFPTANIQPSDDYL
                     LPRKGVYAVSIEIGTENKLYRGVANIGVKPTFHDPNKAEVVIEVNIFDFEDNIYGERV
                     TVNWHHFLRPEIKFDGIDPLVKQMNDDKSRAKYLLAVDFGDEVAYNI"
     gene            28741..29010
                     /gene="rpsO"
                     /locus_tag="POB55_000506"
     CDS             28741..29010
                     /gene="rpsO"
                     /locus_tag="POB55_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HDI6499498.1"
                     /translation="MAISQERKNEIIKEYRVHETDTGSPEVQIAVLTAEINAVNEHLR
                     THKKDHHSRRGLLKMVGRRRHLLNYLRSKDIQRYRELIKSLGIRR"
BASE COUNT        10765 a         4008 c         5723 g         8624 t
ORIGIN      
        1 ttgtaattta ttttttaata aaatctataa aaaatagaca gggaaaatga tttgtttaga
       61 tataaaacgt tgacaaaagc aaaattaagc gtttatcatt tatctttagt aattagatta
      121 gcgaggggga aatgacattg gcagataatt tagtcattgt tgaatcgcct gcaaaagcaa
      181 aaaccattga aaagtattta ggtaagaaat ataaagttat agcttcaatg ggacacgtca
      241 gagacttacc aagaagtcaa atgggtgtcg acactgaaga taattacgaa ccaaaatata
      301 taacaatacg cggaaaaggt cctgttgtaa aagaattgaa aaaacatgca aaaaaagcga
      361 aaaacgtctt tctcgcaagt gaccccgacc gtgaaggtga agcaattgct tggcatttat
      421 caaaaatttt agagcttgaa gattctaaag aaaatcgcgt tgttttcaac gaaataacta
      481 aagacgctgt taaagaaagt tttaaaaatc ctagagaaat tgaaatgaac ttagtcgatg
      541 cacaacaagc gcgtcgaata ttagatagat tggttggcta taacatctcg ccagttctat
      601 ggaaaaaagt aaaaaaaggt ttgtcagcgg gtcgagttca atctgttgca cttcgtttag
      661 tcattgaccg tgaaaatgaa attcgaaact ttaaaccaga agaatattgg actattgaag
      721 gagaatttag atacaaaaaa tcaaaattca atgctaaatt ccttcattat aaaaataaac
      781 cttttaaatt aaaaacgaaa aaagatgttg agaaaattac agctgcacta gatggagatc
      841 aattcgaaat tacaaacgtg actaaaaaag aaaaaacgcg taatccagca aacccattta
      901 caacttctac attacaacaa gaggcggcac gtaaattaaa ctttaaagca agaaaaacaa
      961 tgatggtcgc acaacaatta tatgaaggta tagatttgaa aaaacaaggt acgattggtt
     1021 taataacata tatgagaacc gattctatac gtatttcaga tactgccaaa gctgaagcaa
     1081 aacagtatat aactgataaa tacggtgaat cttacacttc taaacgtaaa gcatcaggga
     1141 aacaaggtga ccaagatgcc catgaggcta ttagaccttc aagtactatg cgtacgccag
     1201 atgatatgaa gtcatttttg acgaaagacc aataccgatt atacaaatta atttgggaac
     1261 gatttgttgc tagtcaaatg gctccagcaa tacttgatac agtctcatta gacataacac
     1321 aaggtgacat taaatttaga gcgaatggtc aaacaatcaa gtttaaagga tttatgacac
     1381 tttatgtaga aactaaagat gatagtgata gcgaaaagga aaataaactg cctaaattag
     1441 agcaaggtga taaagtcaca gcaactcaaa ttgaaccagc tcaacactat acacaaccac
     1501 ctccaagata tactgaggcg agattagtaa aaacactaga agaattgaaa attgggcgac
     1561 catcaactta tgcaccgaca atagatacga ttcaaaagcg taactatgtc aaattagaaa
     1621 gtaagcgttt tgttcctact gagttgggag aaatagttca tgaacaagtg aaagaatact
     1681 tcccagagat tattgatgtg gaattcacag tgaatatgga aacgttactt gataagattg
     1741 cagaaggcga cattacatgg agaaaagtaa tcgacggttt ctttagtagc tttaaacaag
     1801 atgttgaacg tgctgaagaa gagatggaaa agattgaaat caaagatgag ccagccggtg
     1861 aagactgtga agtttgtggt tctcctatgg ttataaaaat gggacgctat ggtaagttca
     1921 tggcttgctc aaacttcccg gattgtcgta atacaaaagc gatagttaag tctattggtg
     1981 ttaaatgtcc aaaatgtaat gatggtgacg tcgtagaaag aaaatctaaa aagaatcgtg
     2041 tcttttatgg atgttcgaaa tatcctgaat gcgactttat ctcttgggat aagccgattg
     2101 gaagagattg tccaaaatgt aaccaatatc ttgttgaaaa taaaaaaggc aagacaacac
     2161 aagtaatatg ttcaaattgc gattataaag aggcagcgca gaaataatat ttttatttcc
     2221 tagatacatt ttaaggttgt taaatagaat cattagtgaa tcttatttta aagatagtaa
     2281 tggattaatc taaataagtg cggataatat aaacataaca acataattaa aagacataaa
     2341 tgacaataaa aggagtatag aaatgactca aactgtaaat gtaataggtg ctggtcttgc
     2401 cggttcagaa gcggcatatc aattagctga aagaggaatt aaagttaatc taatagagat
     2461 gagacctgtt aaacaaacac cagcgcacca tactgataaa tttgcggaac ttgtatgttc
     2521 caattcatta cgaggaaatg ctttaactaa tggtgtgggt gttttaaaag aagaaatgag
     2581 aagattgaat tctataatta ttgaagcggc tgataaggca cgagttccag ctggtggtgc
     2641 attagcagtt gatagacacg atttttcagg ttatattact gaaacactta aaaatcatga
     2701 aaatatcaca gttattaatg aagaaattaa tgccattcca gatggataca caattatcgc
     2761 aacaggacca cttactacag aaacccttgc gcaagaaata gtggacatta ctggtaaaga
     2821 tcaactttat ttctatgatg cggctgctcc aattattgaa aaagaatcta ttgatatgga
     2881 taaagtttac ttaaagtccc gttatgataa aggtgaagct gcatatttaa actgtcctat
     2941 gactgaggat gaatttaatc gcttttatga tgcagtatta gaagctgaag ttgcgcctgt
     3001 aaattcattt gaaaaagaaa aatatttcga gggttgtatg ccttttgaag taatggcaga
     3061 acgcggacgc aagacattac tatttggacc aatgaaacca gtaggattag aagatccaaa
     3121 gactgggaaa cgtccttatg cagtggttca attaagacaa gatgatgctg ctggcacact
     3181 ctacaatatt gtcggcttcc aaacgcattt aaaatgggga gctcaaaaag aagttattaa
     3241 attaattcca ggtttagaaa atgttgatat tgttagatat ggtgtgatgc atagaaatac
     3301 cttcattaac tcaccggacg tattaaacga gaaatatgaa ttgatttcac aaccaaacat
     3361 acagtttgcg ggacaaatga ctggtgttga aggttatgta gaaagcgcag ctagcggctt
     3421 agttgcaggt atcaatcttg cgcataaaat attaggcaaa ggtgaggtag tatttcctag
     3481 agaaacgatg attggaagta tggcttacta tatttctcat gctaaaaata ataagaattt
     3541 ccaacctatg aatgctaact tcgggttatt accatcttta gaaactagaa ttaaagataa
     3601 aaaagaacgc tatgaagcac aagctaatag agctttggat tacttagaaa atttcaaaaa
     3661 aactttataa aatagttaga aagactagat atgctattca ttcttaagtc atcaacgagt
     3721 aagtaatgac tttctaaatg gaaaatactt atcctagtct ttttaatttt ggaattgtta
     3781 cgtatttctg acaatttaga attcgcattc aaaaaatatc taaataaata acacgcaata
     3841 agttgattga tgtaacatgt aagagaatgt tttaaataaa ctttatttaa aaggcaatga
     3901 aataataaat ggcaaggcta ttaataaaga cttttagtaa ttaatttaaa aaagaggtat
     3961 tctaattaac aggttttccg attagttaca attatttaat tctcaaaaga tttagaattg
     4021 attatcaaat tactgtaagc cctttgctgt atatgctaca attcttattg atggagggta
     4081 aatgtattga atcatattca agaagcgttt ttaaatacat tgaaagttga acggaatttt
     4141 tcggaacata cattgaaatc atatcaagat gacttaattc agtttaatca atttttagaa
     4201 caagaacatt tacagttgaa aacttttgaa tacagagatg ctagaaatta tttgagctat
     4261 ttatattcaa atcatttgaa aagaacatct gtttctcgta aaatctcaac gttaagaact
     4321 ttctatgaat attggatgac gcttgatgag aacattatta atccatttgt tcaattagta
     4381 catccgaaaa aagaaaaata tcttccgcaa ttcttttacg aagaagaaat ggaagcgtta
     4441 tttaaaactg tagaagagga cacttcaaaa agtttacggg atcgagttat tcttgaattg
     4501 ttatatgcta caggtatccg tgtttcggaa ttagttaata ttaaaaaaca agatatagat
     4561 ttttacgcga atggtgttac cgtattagga aaagggagca aagagcgctt tgtaccgttt
     4621 ggtgcttatt gtagacaaag catcgaaaat tatttagaac atttcaaacc aattcagtca
     4681 tgcaatcatg attttcttat tgtaaatatg aagggtgaag caatcactga acgcggtgta
     4741 cgatatgttt taaatgatat tgttaaacga acagcaggcg taagtgagat tcatccccac
     4801 aagctcagac atacatttgc aacgcattta ttgaatcaag gtgcagacct aagaacagta
     4861 caatcgttat taggtcatgt taatttgtca acaactggta aatatacaca cgtatctaac
     4921 caacaattaa gaaaagtgta tctaaatgca catcctcgag cgaaaaagga gaatgaaaca
     4981 tgagtaatac aacattacat gcaacaacaa tttatgctgt aagacataat gggaaagcag
     5041 ctatggctgg agatgggcaa gtaacgcttg gtcaacaagt catcatgaaa caaacggcaa
     5101 gaaaagtgcg acgtttatat gaaggtaaag tgttagctgg tttcgcaggt agtgtagcag
     5161 atgcgtttac gttatttgaa aaattcgaaa caaaattaca acagtttagt ggtaacttag
     5221 aaagagctgc tgttgaattg gcacaagaat ggcgaggcga taaacaatta cgtcaattag
     5281 aagctatgct aattgtaatg gataaagatg ctattttagt tgtcagtgga actggcgaag
     5341 ttattgcccc agatgatgac cttatcgcta ttggatcagg aggcaactac gcattaagcg
     5401 caggacgtgc attgaaacgc catgcatcgc atttgtctgc tgaagaaatg gcatatgaga
     5461 gcttgaaagt agcggctgat atttgtgtct ttaccaacga taatattgtt gttgaaacac
     5521 tataataatc agagcacgat aaataattac gagcaattaa ttttagttaa aagacggagg
     5581 aatgaaatta atggatacag ctggaataag attaactcca aaagaaatcg tatctaaatt
     5641 aaatgaatac atcgttggac aaaatgatgc taaacgtaaa gtggcaattg ccttacgtaa
     5701 tcgatacaga agaagtttat tagatgagga atcaaagcaa gaaatttcac ctaaaaatat
     5761 tttgatgatt ggaccaactg gcgttggtaa aactgaaatt gcaagaagaa tggccaaagt
     5821 tgtcggcgcg ccatttataa aagtagaagc tactaaattt actgaggtag gttatgtagg
     5881 acgagatgtt gaaagtatgg ttagagatct tgttgatgtt tcagtaagat tagtcaaggc
     5941 acagaaaaaa tcattggtac aagatgaagc aacagctaag gccaatgaaa aacttgttaa
     6001 gttattagtt ccaagtatga aaaagaaagc gtctcaaacg aataatcctt tagagtcact
     6061 tttcggaggt gcaattccaa atttcggaca aaataacgaa gatgaagaag aaccacctac
     6121 tgaggaaatt aaaacaaaac gttctgaaat taagagacag ctagaagaag gcaaacttga
     6181 aaaagaaaag gtaagaatta aagtcgaaca agatcctggt gctttaggta tgctaggtac
     6241 aaatcaaaat cagcaaatgc aagagatgat gaatcaatta atgcctaaaa agaaagttga
     6301 gcgagaagtt gctgttgaga cggcaaggaa aatcttagct gatagttatg cggatgaact
     6361 aattgatcaa gaaagcgcta accaagaagc gcttgaatta gcagaacaaa tgggtatcat
     6421 ctttatagat gaaatcgaca aagttgcgac gaataatcat aatagtggtc aagatgtctc
     6481 aagacaaggt gttcaaagag atattttacc tatacttgaa ggtagcgtta ttcaaaccaa
     6541 atatggtact gtgaatactg aacatatgct gtttataggt gctggagctt tccatgtatc
     6601 taagccgagt gacttgatac cagaattgca aggtcgtttt ccgattagag ttgaacttga
     6661 tagtttatcg gtagaagatt ttgtaagaat tttgacagaa ccaaaattgt cattaattaa
     6721 acaatatgaa gcattgcttc aaacagaaga agttactgta aactttaccg atgaagcaat
     6781 tactcgttta gctgagattg cttatcaagt aaatcaagat acagacaaca ttggtgcacg
     6841 tcgacttcat acaattttag aaaagatgtt agaagattta tcattcgaag caccaagtat
     6901 gccgaatgca gttgtagata ttaccccaca atatgttgat gataaattaa aatcaatttc
     6961 aacaaataaa gatttaagtg catttattct ataaaaatat acaaaaggag aaaaattcat
     7021 gagcttatta tctaaaacga gagagttaaa cacgttactt caaaaacaca aaggtattgc
     7081 ggttgatttt aaagatgtag cacaaacgat tagtagcgta actgtaacaa atgtatttat
     7141 tgtatcgcgt cgaggtaaaa ttttagggtc gagtctaaat gaattattaa aaagtcaaag
     7201 aattattcaa atgttggaag aaagacatat cccaagtgaa tatacagaac gattaatgga
     7261 agttaaacaa acagaatcaa atattgatat cgacaatgta ttaacagttt ttccacctga
     7321 aaacagagaa ttattcatag atagtcgtac aactatcttc ccaattttag gtggaggaga
     7381 aagattaggt acattagtac ttggtcgagt acatgatgat tttaatgaaa atgatttggt
     7441 actaggtgaa tatgctgcta cagttattgg tatggaaatc ttacgtgaga agcatagtga
     7501 agtagaaaaa gaagcgcgcg ataaagctgc tattacaatg gcaattaatt cattatctta
     7561 ttctgaaaaa gaagcgattg aacatatctt tgaagaactt ggcggtacgg aaggcctatt
     7621 aatcgcatca aaagttgcag atagagttgg tattactaga tctgtaattg taaatgcact
     7681 acgtaaatta gaaagtgctg gtgtaattga atcacgttct ttaggaatga aaggtacttt
     7741 cattaaagtt aaaaaagaaa aattcttaga tgaattagaa aaaagtaaat aagtcgatga
     7801 gtctgggaca taattcaatg tcctagtcgt gacgatgtta tcttggcggt agtcaacgaa
     7861 atgactttga tgattatgtt gaagtcattt ctttattttt gtctaatttt caatcaaatt
     7921 gatattccac attaacatga tagaattacc tttaaattta tgaaaaaatt cttgaaaaac
     7981 atttgcttta gacacggcat tatgttatat ttattttcgg ctataaagcc aatacacaca
     8041 tatcaaacgt gattgtgtaa agggtgcaat atttgtatat tgtctttaca atagtttgct
     8101 atggaggtaa ttaaccaata ggaggaattt ataatggcag taatttcaat gaaacaatta
     8161 ctagaagcgg gtgttcactt cggtcaccaa acacgtcgtt ggaacccaaa aatgaaaaaa
     8221 tatatcttca ctgagagaaa tggtatttat atcatcgact tacaaaaaac agtgaaaaaa
     8281 gtagacgagg catacaactt cttgaaacaa gtttcagaag atggtggaca agtcttattc
     8341 gtaggaacta aaaaacaagc acaagaatca gttaaatctg aagcagaacg tgctggtcaa
     8401 ttctacatta accaaagatg gttaggtgga ttattaacta actataaaac gatctcaaaa
     8461 cgaatcaaac gtatttctga aattgaaaaa atggaagaag atggtttatt cgaagtatta
     8521 cctaaaaaag aagtagtaga acttaaaaaa gaatacgacc gtttaatcaa attcttaggc
     8581 ggaattcgtg atatgaaatc aatgcctcaa gcattattcg tagttgaccc acgtaaagag
     8641 cgtaatgcaa ttgctgaagc tcgtaaatta aatattccta tcgtaggtat cgttgacact
     8701 aactgtgatc ctgacgaaat tgactacgtt atcccagcaa acgacgatgc tatccgtgcg
     8761 gttaaattat taactgctaa aatggcagat gcaatcttag aaggtcaaca aggcgtttct
     8821 aatgaagaag tagctgcaga acaaaacatc gatttagatg aaaaagaaaa atcagaagaa
     8881 acagaagcaa ctgaagaata atcaactgtt gaatctgact tagatatagt ttaaatgggt
     8941 gataagatat taatgcttat cacctttttt aaaaagaaaa tcgaggcaaa ttacaaatat
     9001 tcaattagag tattagcaat cttgcctata ataatgctaa aatcataata tataaaatga
     9061 taacttattg gaggaataat gaatggcaac tatttcagca aaacttgtta aagaattacg
     9121 tgaaaaaact ggcgcgggta tgatggattg taaaaaagcg ctaactgaaa ctgatggtga
     9181 catcgataaa gcgattgatt acctacgtga aaaaggtatt gctaaagcag ctaaaaaagc
     9241 agaccgtatt gcggctgaag gtttagtaca tgtagaaact aaaggtaacg acgcagttat
     9301 cgttgaaatc aactctgaaa cagactttgt tgctcgtaac gaaggattcc aagagttagt
     9361 taaagaaatc gctaatcaag tattagatac aaaagctgaa actgttgaag ctttaatgga
     9421 aacaacttta ccaaatggta aatcagttga tgaaagaatt aaagaagcaa tttcaacaat
     9481 cggtgaaaaa ttaagtgttc gtcgttttgc tatcagaact aaaactgata acgatgcttt
     9541 cggcgcttac ttacacatgg gtggacgcat tggtgtatta acagttgttg aaggttcaac
     9601 tgacgaagaa gcagcaagag acgttgctat gcatatcgct gcaatcaacc ctaaatatgt
     9661 ttcttctgaa caagttagcg aagaagaaat caaccacgaa agagaagttt taaaacaaca
     9721 agcattaaat gaaggtaaac cagaaaacat cgttgaaaaa atggtggaag gacgtttacg
     9781 taaatactta caagaaattt gtgctgtaga tcaagacttc gttaaaaacc ctgatgtaac
     9841 agttgaagct ttcttaaaaa caaaaggtgg aaaacttgtt gacttcgtac gctatgaagt
     9901 aggcgaaggt atggaaaaac gcgaagaaaa ctttgcggat gaagttaaag gacaaatgaa
     9961 ataatctgtc ataaagtaaa acaaggaaga agacaccttt aatgttgctt tattaaaatg
    10021 taaatcattc taataaaacg acaactgtgt cttctttact tgtatatgtt acatatattc
    10081 acgatagaga ggataagaaa atggctcaaa tttctaaata taaacgtgta gttttgaaac
    10141 taagtggtga agcgttagct ggagaaaaag gatttggcat aaatccagta attattaaaa
    10201 gtgttgctga gcaagtggct gaagttgcta aaatggactg tgaaatcgca gtaatcgttg
    10261 gtggcggaaa catttggaga ggtaaaacag gtagtgactt aggtatggac cgtggaactg
    10321 ctgattacat gggtatgctt gcaactgtaa tgaatgcctt agcattacaa gatagtttag
    10381 aacaattgga ttgtgataca cgagtattaa catctattga aatgaagcaa gtggctgaac
    10441 cttatattcg tcgtcgtgca attagacact tagaaaagaa acgcgtagtt atttttgctg
    10501 caggtattgg aaacccatac ttctctacag atactacagc ggcattacgt gctgcagaag
    10561 ttgaagcaga tgttatttta atgggcaaaa ataatgtaga tggtgtatat tctgcagatc
    10621 ctaaagtaaa caaagatgcg gtaaaatatg aacatttaac gcatattcaa atgcttcaag
    10681 aaggtttaca agtaatggat tcaacagcat cctcattctg tatggataat aacattccgt
    10741 taactgtttt ctctattatg gaagaaggaa atattaaacg tgctgttatg ggtgaaaaga
    10801 taggtacgtt aattacaaaa taaatttaga ggtgtaaaat aatgagtgac attattaatg
    10861 aaactaaatc aagaatgcaa aaatcaatcg aaagcttatc acgtgaatta gctaatatca
    10921 gtgcaggaag agctaattca aatttattaa acggcgtaac agttgattac tatggtgcac
    10981 caacacctgt acaacaatta gcaagcatca atgttccaga agcacgttta cttgttattt
    11041 ctccatacga caaaacttct gtagctgaca tcgaaaaagc gataatagcg gctaacttag
    11101 gtgtcaaccc aacaagtgat ggtgaagtga tacgtattgc agtacctgcc ttaacagaag
    11161 agcgtagaaa agagcgcgtt aaagatgtta agaaaattgg tgaagaagct aaagtatctg
    11221 ttcgaaatat tcgtcgtgat atgaatgatc agttgaaaaa agatgaaaaa aatggcgaca
    11281 ttactgaaga tgagttgaga agtggtactg aagatgttca gaaagcaaca gacaattcaa
    11341 taaaagaaat tgatcaaatg attgctgata aagaaaaaga tattatgtca gtataaaact
    11401 aatatacaat gacatattaa aatgccagta ttaaacgata atgtaacatt taaaatgggc
    11461 atgtttaatt aaatcaaaga tgcatgtgat gatttaaatt cacaatgagc ataaaaatgg
    11521 tgtttaaaca agttaattaa acatatactt tataaataat aggcattagg tatattgcta
    11581 taataaagtt atgtaatttt taacctcagt atgtatgtca catttctggt gtaaactgta
    11641 ccgagtcaga ctttggtaca gtttttttat ttgcttattc aatgcattaa atgagtatga
    11701 taaaatgata atgattgttt agtaacttat actatatgac agagatgatc aggctcggag
    11761 gaaagaccat gtttaaaaag ctaataaata aaaagaacac tataaataat tataatgaag
    11821 aattagactc gtctaatata cctgaacata tcgctattat tatggatggt aatgggcgat
    11881 gggctaagaa gcgaaaaatg cctagaatta aaggtcatta cgaaggtatg caaacaataa
    11941 aaaaaattac tagggtagct agtgatattg gtgttaagta cttaacttta tacgcctttt
    12001 ccactgaaaa ttggtcaaga cctgaaagtg aagtaaatta tattatgaat ttgcctgtca
    12061 atttcttaaa gacattctta ccggaactaa ttgaaaaaaa tgtcaaagtt gaaacaattg
    12121 gatttactga taagttgcca aaatcaacga tagaagcaat taataatgct aaagaaaaga
    12181 cagctaataa taccggctta aaattaatat ttgcaattaa ttatggtggc agagcagaac
    12241 ttgttcatag tattaaaaat atgtttgacg agcttcatca acaaggttta aatagtgata
    12301 tcatagatga aacatatata aacaatcatt taatgacaaa agactatcct gatccagagt
    12361 tgttaattcg tacttcagga gaacaaagaa taagtaattt cttgatttgg caagtttcgt
    12421 atagtgaatt tatctttaat caaaaattat ggcctgactt tgacgaagat gaattaatta
    12481 aatgtataaa aatttatcag tcacgtcaaa gacgctttgg cggattgagt gaggagtagt
    12541 atagtatgaa agttagaacg ctgacagcta ttattgcctt aatcgtattc ttgcctatct
    12601 tgttaaaagg cggccttgtg ttaatgatat ttgctaatat attagcattg attgcattaa
    12661 aagaattgtt gaatatgaat atgattaaat ttgtttcagt tcctggttta attagtgcag
    12721 ttggtcttat catcattatg ttgccacaac atgcagggcc atgggtacaa gtaattcaat
    12781 taaaaagttt aattgcaatg agctttattg tattaagtta tactgtctta tctaaaaaca
    12841 gatttagttt tatggatgct gcattttgct taatgtctgt ggcttatgta ggcattggtt
    12901 ttatgttctt ttatgaaacg agatcagaag gattacatta catattatat gcctttttaa
    12961 ttgtttggct tacagataca ggggcttact tgtttggtaa aatgatgggt aaacataagc
    13021 tttggccagt aataagtccg aataaaacaa tcgaaggatt cataggtggc ttgttctgta
    13081 gtttgatagt accacttgca atgttatatt ttgtagattt caatatgaat gtatggatat
    13141 tacttggagt gacattgatt ttaagtttat ttggtcaatt aggtgattta gtggaatcag
    13201 gatttaagcg tcatttcggc gttaaagact caggtcgtat actacctgga cacggtggta
    13261 ttttagaccg atttgacagc tttatgtttg tgttaccatt attaaatatt ttattaatac
    13321 aatcttaatg ctgagaacaa atcaataaac gtaaagagga gttgctgaga taatttaatg
    13381 aatctcagaa ctccttttga aaattatacg caatattaac tttgaaaatt agacgttata
    13441 ttttgtgatt tgtcagtatc atattataat gacttatgtt acgtatacag caatcatttt
    13501 taaaataaaa gaaatttata aacaatcgag gtgtagcgag tgagctattt agttacaata
    13561 attgcattta ttattgtttt tggtgtacta gtaactgttc atgaatatgg ccatatgttt
    13621 tttgcgaaaa gagcaggcat tatgtgtcca gaatttgcga tcggtatggg accgaaaatt
    13681 tttagtttta gaaaaaacga aacactttac actattaggt tattgcctgt tggtggatat
    13741 gttcgtatgg caggagatgg cttagaagag ccaccagtcg agcccggtat gaacgttaaa
    13801 attaaactga atgaagaaaa tgaaataaca catatcatat tagatgatca tcataagttt
    13861 caacaaattg aagcaatcga agttaaaaaa tgtgatttta aggatgactt attcatagaa
    13921 ggtatcactg cttatgataa tgaaagacat cattttaaaa ttgctagaaa gtctttcttt
    13981 gttgaaaatg gtagcttagt tcaaattgct ccgagagaca gacaatttgc acataaaaag
    14041 ccatggccga aatttttaac attatttgcg ggaccgttat ttaactttat attagcttta
    14101 gtcctattta ttggtcttgc atattatcaa ggcacgccta cgtctactgt agaacaagtc
    14161 gcagataagt atccagctca acaagcagga ttacaaaaag gtgataagat cgtccaaatt
    14221 ggcaaatata aaatatctga atttgatgat gttgataagg cgttagataa agttaaagat
    14281 aataagacga ctgttaaatt tgaacgtgat ggtaaaacaa agtcagttga attaacacct
    14341 aaaaagactg aaagaaaact gactaaagta agttcagaga cgaagtatgt tctcggattc
    14401 caaccagcga gtgaacgtac gctcttcaaa ccaattgtat atggatttga aagctttttg
    14461 aaaggtagta cactaatttt tacagctgta gtcggtatgt tggctagtat atttacgggc
    14521 ggattctcat ttgatatgtt aaatggtccg gttggtattt atcataacgt cgactcagtt
    14581 gttaaagcgg gtatcattag cttaattggt tacactgcgt tattaagtgt aaacttaggt
    14641 attatgaatt taattcctat tcctgcacta gacggtggtc gtattttatt tgttatatat
    14701 gaagcgattt tcagaaaacc agttaataaa aaagcggaaa caacgattat tgctattggt
    14761 gccattttca tggtcgttat tatgatatta gtaacgtgga atgatattcg acgatatttc
    14821 ttataattta ggaggataaa taattatgaa gcaatccaaa gtttttatac caacgatgcg
    14881 tgatgtgcca tcagaagcag aagcacaaag tcatcgttta ttattgaaat cgggtttgat
    14941 aaaacaaagt acaagtggga tttatagtta tttaccgcta gcaacacgtg tgttaaataa
    15001 tattactgca attgtgcgac aagaaatgga acgtatcgat tctgttgaaa ttttaatgcc
    15061 agcgttacaa caagctgaat tatgggaaga atcaggacgt tggggtgcat atggcccaga
    15121 attaatgcgt ttacaagata gacatggaag acaatttgca ttaggtccaa cacatgaaga
    15181 attagttaca tcaatagtaa gaaatgaatt gaaatcatac aaacaattac cgatgacatt
    15241 attccaaatt caatctaaat tccgtgatga aaagagacca cgttttggtt tacttcgtgg
    15301 gcgtgaattt attatgaaag atgcgtattc attccatgct gacgaggcat cattagatca
    15361 aacgtatcaa gatatgtatc aagcgtatag ccgtattttt gagagagttg gcattaacgc
    15421 aagaccagta gttgcagatt caggtgctat aggcggtagc catacacatg aatttatggc
    15481 attaagtgct atcggtgagg atacaatcgt ttacagtaaa gaaagtgact atgctgctaa
    15541 tatcgaaaaa gcagaagtcg tttacgaacc aaatcataag tattctactg tgcaaccttt
    15601 agaaaaaatt gaaacaccaa atgttaagac tgcacaagaa ttggcagact tcttaggtag
    15661 accagtagat gaaatcgtta aaacgatgat tttcaaagtt gatggcgaat atattatggt
    15721 tttagtgcgt ggccatcatg aaattaatga cattaaatta aaatcttatt tcggcacaga
    15781 taatattgaa ttagcaacac aagacgaaat tgttaattta gttggtgcaa atccgggttc
    15841 actaggtcct gttattgata aagaaatcaa aatttatgca gataattttg tgcaagattt
    15901 aaataattta gttgtcggtg ctaacgaaga tggctatcac ttaattaatg taaatgtagg
    15961 tagagacttc aacgttgatg aatatggcga tttccgtttt attttagaag gcgaaaagtt
    16021 aagtgatggt tcaggcgttg cacattttgc tgaaggtatt gaagttggtc aagtattcaa
    16081 attgggtact aagtattcag aatcaatgaa tgctacattc ttagataacc aaggaaaagc
    16141 ccaaccttta attatgggtt gttacggtat tggaatttct agaacgctga gtgcgattgt
    16201 tgaacaaaat cacgatgata atggaattgt ttggcctaaa tcagttactc catttgattt
    16261 acatttaatt tctattaatc ctaagaaaga tgatcaacga gaactagcgg atgcactata
    16321 tgctgaattt aatactaaat ttgatgtgtt gtacgatgat cgtcaggaac gtgcgggtgt
    16381 caaatttaat gatgccgatt taattggttt accactgcga attgttgttg gtaaacgtgc
    16441 atcggaaggt attgtagaag ttaaagaacg tttaacaggt gatagcgaag aagttcacat
    16501 tgatgactta atgactgtca ttacaaataa atatgataac ttaaaataat taagatcgaa
    16561 tgaattataa gagtaggaaa aagctgaaag aaatcagatg cttatgtcct gctcttatta
    16621 tttttgatat aatgattatt cgatgaaaaa tgactgaaga catagtataa ttaaagataa
    16681 atttgtttta acaatattat gattagccaa atataaagca tttaattttc tatcattact
    16741 atgcctacat aatctaaata ttgttcgaac acgtaaaagt aatttctatt taaggtggta
    16801 attgtcttgg caatgacaga gcaacaaaaa tttaaagtgc ttgctgatca aattaaaatt
    16861 tcaaatcaat tagatgctga aattttaaat tcaggtgaac tgacacgtat agatgtttct
    16921 aacaaaaaca gaacatggga atttcatatt acattaccac aattcttagc tcatgaagat
    16981 tatttattat ttataaatgc aatagagcaa gagtttaaag atatcgccaa cgttacatgt
    17041 cgttttacgg taacaaatgg cacgaatcaa gatgaacatg caattaaata ctttggacac
    17101 tgtattgacc aaacagcttt atctccaaaa gttaaaggtc aattgaaaca gaaaaagctt
    17161 attatgtctg gaaaagtatt aaaagtaatg gtatcaaatg acattgaacg taatcatttt
    17221 gataaggcat gcaatggaag tcttatcaaa gcgtttagaa attgtggttt tgatatcgat
    17281 aaaatcatct tcgaaacaaa tgataatgat caagaacaaa acttagcttc tttagaagca
    17341 catattcaag aagaagacga acaaagtgca cgattggcaa cagagaaact tgaaaaaatg
    17401 aaagctgaaa aagcgaaaca acaagataac aacgaaagtg ctgttgataa gtgtcaaatt
    17461 ggtaagccga ttcaaattga aaatattaaa ccaattgaat ctattattga ggaagagttt
    17521 aaagttgcaa tagagggtgt catttttgat ataaacttaa aagaacttaa aagtggtcgc
    17581 catatcgtag aaattaaagt gactgactat acggactctt tagttttaaa aatgtttact
    17641 cgtaaaaaca aagatgattt agaacatttt aaagcgctaa gtgttggtaa atgggttagg
    17701 gctcaaggtc gtattgaaga agatacattt attagagatt tagttatgat gatgtctgat
    17761 attgaagaga ttaaaaaagc gacaaaaaaa gataaggctg aagaaaagcg tgtagaattc
    17821 cacttgcata ctgcaatgag ccaaatggat ggtataccca atattggtgc gtatgttaaa
    17881 caggcagcag actggggaca tccagccatt gcggttacag accataatgt tgtgcaagca
    17941 tttccagatg ctcacgcagc agcggaaaaa catggcatta aaatgatata cggtatggaa
    18001 ggtatgttag ttgatgatgg tgttccgatt gcatacaaac cacaagatgt cgtattaaaa
    18061 gatgctactt atgttgtgtt cgacgttgag acaactggtt tatcaaatca gtatgataaa
    18121 atcatcgagc ttgcagctgt gaaagttcat aacggtgaaa tcatcgataa gtttgaaagg
    18181 tttagtaatc cgcatgaacg attatcggaa acgattatca atttgacgca tattactgat
    18241 gatatgttag tagatgcccc tgagattgaa gaagtactta cagagtttaa agaatgggtt
    18301 ggcgatgcga tattcgtagc gcataatgct tcgtttgata tgggctttat cgatacggga
    18361 tatgaacgtc ttgggtttgg accatcaacg aatggtgtta tcgatacttt agaattatct
    18421 cgtacgatta atactgaata tggtaaacat ggtttgaatt tcttagctaa aaaatatggc
    18481 gtagaattaa cgcaacatca ccgtgccatt tatgatacag aagcaacagc ttacattttc
    18541 ataaaaatgg ttcaacaaat gaaagaatta ggcgtattaa atcataacga aatcaacaaa
    18601 aaactcagta atgaagatgc atataaacgt gcaagaccta gtcatgtcac attaattgta
    18661 caaaaccaac aaggtcttaa aaatctattt aaaattgtaa gtgcatcatt ggtgaagtat
    18721 ttctaccgta cacctcgaat tccacgttca ttgttagatg aatatcgtga gggattattg
    18781 gtaggtacag cgtgtgatga aggtgaatta tttacggcag ttatgcagaa ggaccagagc
    18841 caagttgaaa aaattgccaa atattatgat tttattgaaa ttcaaccacc ggcactttat
    18901 caagatttaa ttgatagaga gcttattaga gatactgaaa cattacatga aatttatcaa
    18961 cgtttaatac atgcaggtga cacagcgggt atacctgtta ttgcgacagg aaatgcacac
    19021 tatttgtttg aacatgatgg tatcgcacgt aaaattttaa tagcatcaca acccggcaat
    19081 ccacttaatc gctcaacttt accggaagca cattttagaa ctacagatga aatgttaaac
    19141 gagtttcatt ttttaggtga agaaaaagcg catgaaattg ttgtgaaaaa tacaaacgaa
    19201 ttagcagatc gaattgaacg tgttgttcct attaaagatg aattatacac accgcgtatg
    19261 gaaggtgcta acgaagaaat tagagaacta agttatgcaa atgcgcgtaa actgtatggt
    19321 gaagacctgc ctcaaatcgt aattgatcga ttagaaaaag aattaaaaag tattatcggt
    19381 aatggatttg cggtaattta cttaatttcg caacgtttag ttaaaaaatc attagatgat
    19441 ggatacttag ttggttcccg tggttcagta ggttctagtt ttgtagcgac aatgactgag
    19501 attactgaag taaacccgtt accgccacac tatatttgtc cgaactgtaa aacgagtgaa
    19561 tttttcaatg atggttcagt aggatcagga tttgatttac ctgataagac gtgtgaaact
    19621 tgtggagcgc cacttattaa agaaggacaa gatattccgt ttgaaacatt tttaggattt
    19681 aagggagata aagttcctga tatcgactta aactttagtg gtgaatatca accgaatgcc
    19741 cataactaca caaaagtatt atttggtgag gataaagtat tccgtgcagg tacaattggt
    19801 actgttgctg aaaagactgc ttttggttat gttaaaggtt atttgaatga tcaaggcatc
    19861 cacaaaagag gtgctgaaat agatcgactc gttaaaggat gtacaggtgt taaacgtaca
    19921 actggacagc atccaggggg tattattgta gtacctgatt acatggatat ttatgatttt
    19981 acgccgatac aatatcctgc cgatgatcaa aattcagcat ggatgacgac acattttgat
    20041 ttccattcta ttcatgataa tgtattaaaa cttgatatac ttggacacga tgatccaaca
    20101 atgattcgta tgcttcaaga tttatcgggc attgatccaa aaacgatacc tgtagatgac
    20161 aaagaagtca tgcaaatatt tagtacacct gaaagtttag gtgttactga agatgaaatt
    20221 ttatgtaaaa caggtacgtt tggggttcca gaattcggta cagggttcgt gcgtcaaatg
    20281 ttagaagata caaagccaac aacattttct gaattagttc aaatctcagg attatctcat
    20341 ggtacagatg tgtggttagg caatgctcaa gaattaatta aaaccggtat atgtgattta
    20401 tcaagtgtaa ttggttgtcg tgatgatatc atggtttatt taatgtatgc tggtttagaa
    20461 ccatcaatgg cttttaaaat aatggagtca gtacgtaaag gtaaaggttt aactgaagaa
    20521 atgattgaaa cgatgaaaga aaatgaagtg ccggattggt atttagattc atgtcttaaa
    20581 attaagtaca tgttccctaa agcccatgca gcagcatacg ttttaatggc agtacgtatc
    20641 gcatatttca aagtacatca tccactttat tactatgcat cttactttac aattcgtgcg
    20701 tcagactttg atttaatcac gatgattaaa gataaaacaa gcattcgaaa tactgtaaaa
    20761 gacatgtatt ctcgctatat ggatctaggt aaaaaagaaa aagacgtatt aacagtcttg
    20821 gaaattatga atgaaatggc gcatcgaggt tatcgcatgc aaccgattag tttagaaaag
    20881 agtcaggcgt tcgaatttat cattgaaggc gatacactta ttccgccgtt catatcagtg
    20941 cctgggcttg gcgaaaacgt tgcgaaacga attgttgaag ctcgtgacga tggcccattt
    21001 ttatcaaaag aagatttaaa caaaaaagct ggattatctc agaaaattat tgagtattta
    21061 gatgagttag gctcattacc gaatttacca gataaagctc aactttcgat atttgatatg
    21121 taaaatgaaa taatcaaggt atttatttaa tgcgtatggc gtagttaaag aaatacaaaa
    21181 ttgttgctgg acataaaatt atgcctgtat ttcttttcaa cgtcttacga gtctattcga
    21241 atgtaatggt gaaataaatg aacaaacttt tacaagaatc tctgattaat agtgaagtca
    21301 tttgtttcaa gcataaactt atgctataat taagttgctt aaaaattagt gaactcaggc
    21361 agaagagtgg gagattcccg ctcttttcta tttgccaaaa agggaggcct gtatgagtaa
    21421 aattacagaa caagtagaag tgattgttaa accaattatg gaagacttga attttgaact
    21481 tgtagacgtt gaatatgtca aagagggtag agatcatttt cttagaatct ctattgataa
    21541 agaaggtggc gtagatttaa atgattgtac gctagcttct gaaaaaataa gtgaagctat
    21601 ggatgcaaat gatcctattc ctgaaatgta ttatttagac gtagcgtcac ctggtgcaga
    21661 acgtccaatt aaaaaagaac aagatttcca aaatgcaata actaaacctg tatttgtttc
    21721 tttatatgta ccaattgaag gtgaaaagga atggttaggc attttacaag aagtcaataa
    21781 tgaaacaatt gtagtacaag ttaaaatcaa agcaagaacg aaagatatag agataccgag
    21841 agacaaaata gcaaaagcac gtcacgcagt tatgatttaa cgtgatgagg aggaaaaaac
    21901 gtgtcaagta atgaattatt attagctact gagtatttag aaaaagaaaa gaagattcct
    21961 agagcagtat taattgatgc tattgaagca gctttaatta ctgcatacaa aaagaattat
    22021 gatagtgcaa gaaatgtccg tgtggaatta aatatggatc aaggtacttt caaagttatc
    22081 gctcgtaaag atgttgttga agaagtattt gacgacagag atgaagtgga tttaagtaca
    22141 gcgcttgtta aaaaccctgc atatgaaatt ggtgatatat acgaagaaga tgtaacacct
    22201 aaagattttg gtcgtgtagg tgctcaagca gcgaaacaag cagtaatgca acgtcttcgt
    22261 gatgctgaac gtgaaatttt atttgaagaa tttatagaca aagaagaaga catacttact
    22321 ggaattattg accgtgttga ccatcgttat gtatatgtga acttaggtcg tatcgaagct
    22381 gttttatctg aagcagaaag aagtcctaac gaaaaatata ttcctaacga acgtatcaaa
    22441 gtatatgtta acaaagtgga acaaacgaca aaaggtcctc aaatctatgt ttctcgtagc
    22501 catccaggtt tattaaaacg tttatttgaa caagaagttc cagaaattta cgatggtact
    22561 gtaattgtta aatcagtagc acgtgaagct ggcgatcgct ctaaaattag tgtcttctct
    22621 gaaaacaatg atatagatgc tgttggtgca tgtgttggtg ccaaaggcgc acgtgttgaa
    22681 gctgttgttg aagagctagg tggcgaaaaa atcgacatcg ttcaatggaa tgaagatcca
    22741 aaagtatttg taaaaaatgc tttaagccct tctcaagttt tagaagttat tgttgatgaa
    22801 acaaatcaat ctacaatagt tgttgttcct gattatcaat tgtcattagc gattggtaaa
    22861 agaggacaaa acgcacgtct agctgctaaa ttaaccggct ggaaaattga tattaaatca
    22921 gaaacagatg cgcgcgaagc gggtatctat ccagtagttg aagctgaaaa agtaactgaa
    22981 gaagatgttg ctttagaaga tgctgacaca acagaatcaa ccgaagaggt aaatgatgtt
    23041 tcagttgaaa caaatgtaga gaaagaatct gaataatagg ttggagtgaa gtatctatga
    23101 aaaagaaaaa aattccgatg cgaaaatgta ttctttcaaa tgaaatgcat cccaaaaaag
    23161 atatgattcg tgttgttgtt aataaagaag gcgaaatctt tgcggatgtt actggaaaga
    23221 aacaaggccg tggcgcatat gtttctaaag atgttgctat ggttgaaaaa gcacaacaaa
    23281 aagaaatttt agaaaaatat tttaaagcat ctaaagagca attggatcct gtttataaag
    23341 aaattattag attaatttat agagaagaga tcccaaaatg agtatagatc aaatattaaa
    23401 ctttttagga ttagcaatga gagctggtaa agtaaaaaca ggtgaatcag tcattgttaa
    23461 tgagataaaa aaaggaaatt taaagctcgt tattgttgca aatgatgcgt ctgataatac
    23521 agctaaatta attacagata aatgtaagag ttacaaagtt ccattcagaa agtttggaaa
    23581 tcgaaatgaa ttgggaatag cacttggaaa aggtgagcgt gttaatgtag ggattactga
    23641 cccaggcttt gctaaaaagt tgctatcaat gatagatgaa tatcataagg agtgattata
    23701 tgagtaaaca aagaatttac gaatatgcga aagaattaaa tctaaagagt aaagagatta
    23761 tagatgagtt aaaaagcatg aatattgagg tttcaaatca tatgcaagct ttggaagatg
    23821 accaaattaa agcattagat aaaaagttca aaaaagaaca aaagaacgac aataaacaaa
    23881 gcactcaaaa taatcaccaa aaatcaaaca atcaaaacca aaataaaggg caacaaaaag
    23941 ataacaaaaa gaatcaacaa caaaataata aaggcaacaa aggcaataaa aagaataata
    24001 gaaataataa gaaaaataac aagaataata aaccacaaaa tcaaccagct gctccaaaag
    24061 aaataccatc aaaagtgaca tatcaagaag gtattacagt aggcgaattt gcggataaat
    24121 taaatgttga atcatcagaa attatcaaaa aattattctt acttggtatt gttgctaata
    24181 tcaatcaatc attaaatcaa gaaacaatcg aattaattgc cgatgattat ggcgttgagg
    24241 ttgaagagga agttgtgatt aatgaagaag atttatcaat ctacttcgaa gacgaaaaag
    24301 atgatccaga agcaattgag agaccagcag ttgtaacaat tatgggacat gttgaccatg
    24361 gtaaaactac tttattagat tcaattcgtc atacaaaagt tacagcaggt gaagcaggtg
    24421 gaatcactca acatattggt gcatatcaaa ttgaaaacga tggcaaaaaa atcactttct
    24481 tagatacacc gggacatgct gcatttacaa cgatgcgtgc gcgtggtgca caagtaacag
    24541 atattacaat tttagtagta gcagctgacg atggtgttat gccacaaaca attgaagcaa
    24601 ttaaccatgc taaagaagca gaagtaccaa ttattgttgc agtaaataaa attgataaac
    24661 caacttcaaa tcctgatcga gttatgcaag aattaactga atatggttta attcctgaag
    24721 attggggtgg cgaaacaatt ttcgttccac tttctgcatt aagtggtgat ggtatcgacg
    24781 atttattaga aatgatagga ttagttgcag aagttcaaga acttaaagca aatcctaaaa
    24841 accgtgctgt tggtacagtt atcgaagctg aattagataa atcacgtggt ccttctgcat
    24901 cattattagt acaaaacggt acattaaatg ttggggatgc gattgtagtt ggtaatactt
    24961 acggccgtat tcgtgcaatg gttaatgact taggtcaaag aatcaaaatg gctggtccat
    25021 caacgcctgt tgaaattaca ggtattaatg atgtgccaca agctggggat cgctttgttg
    25081 tatttagtga tgaaaaacaa gctcgtcgta ttggtgaatc aagacacgaa gctagcattg
    25141 ttcaacaacg tcaagaaagt aaaaatgttt cattagataa cctgtttgaa caaatgaaac
    25201 aaggtgaaat gaaagattta aacgttatta ttaaaggtga tgttcaaggt tctgttgaag
    25261 ctttagctgc atcattaatg aaaattgatg ttgaaggcgt aaatgttcgt atcattcata
    25321 cagcggttgg tgcaattaat gagtcagacg tgacacttgc taatgcctca aatggtatta
    25381 tcattggttt caatgttcgt ccagacagtg gtgcaaaacg tgctgcagaa gctgaaaatg
    25441 ttgatatgcg tttacacaga gttatttata atgttatcga agaaattgaa tcagcgatga
    25501 aaggtttact tgatccagaa tttgaagaac aagttatcgg acaagctgaa gttcgtcaaa
    25561 cattcaaagt ttctaaagtt ggtactattg ctggatgtta tgttactgaa ggtaaaatta
    25621 cgcgaaatgc tggtgtacgt attattcgtg atggtattgt tcaatatgaa ggcgaattag
    25681 atacacttaa acgtttcaaa gatgatgcta aggaagttgc aaaaggttat gaatgtggta
    25741 ttacaattga aaactacaat gaccttaaag aaggcgatgt tatcgaagca tttgaaatgg
    25801 ttgaaattaa gcgttaatta aataaattac aagctaaaag tatagttaag attgatatgc
    25861 tccctataaa tattgcactt tttaagtgtc tactttatag ggagcatatt tgatactagc
    25921 ttttggtttt ttattagaat agattaccta ttaaaagtta cgttatatgg acatgatttt
    25981 gtataaaatt ttgtggtggc ctagaatgat ttttaatgac aaaatataat gtcgactatt
    26041 attggaaaat tttctgttga aatgcctatc ttacggcaaa ctttatttga ttttataggc
    26101 ttaatttatt aaaataacgt gtgagctaaa ataattgttt aagcattgtt acactaaaaa
    26161 atgcaaataa caattgaact taaagataaa gaggtgacaa gaatgagcag tatgagagca
    26221 gagcgtgttg gtgaacaaat gaagaaggaa ttaatggata tcatcaacaa taaagtcaaa
    26281 gatcctcgag ttggttttat tacaattaca gatgttgttt taacaaatga tttatcgcag
    26341 gctaaagtat ttttaactgt attaggtaac gataaagaag tagaaaatac atttaaagca
    26401 cttgataaag caaaaggctt cattaagtct gaattaggtt ctagaatgcg attacgtatt
    26461 atgccggaat taatgtatga atatgatcaa tcaatcgaat atggcaataa aattgaacga
    26521 atgattcaag atttacacaa acaagataga taatttagtg ttaggtatct ggaaaatgtt
    26581 tgataatttc ttaatatcgg tatattaaca ttaaacagtt aatacataga tgtgtagaaa
    26641 tagttaacat tttccagttt ttttatgaat aaattcagtt gatacgctat taaaatatat
    26701 tttaaaaaaa gaaggtgact atatgtataa tgggatatta ccagtatata aagagcgcgg
    26761 tttaacaagt catgacgttg tattcaaatt gcgtaaaata ttaaaaacta aaaaaatagg
    26821 tcacacgggt acgcttgatc ccgaagttgc aggcgtgtta ccggtatgta taggtaatgc
    26881 aacgagagtt agtgattatg ttatggatat gggcaaagct tatgaagcaa ctgtatcgat
    26941 aggaagaagt acaacgactg aagatcaaac gggtgataca ttggaaaaaa aaggtgtaca
    27001 ctcagcagat tttaataagg acgatattga ccgattgtta gaaagtttta aaggtatcat
    27061 tgaacaaatt ccgccgatgt actcatccgt caaagtaaat ggtaaaaaat tatatgaata
    27121 tgcgcgtaat aatgaaacag ttgaaagacc aaagcgtaaa gttaatatta aagacattgg
    27181 gcgtatatct gaattagatt ttaaagaaaa tgagtgtcat tttaaaatac gcgtcatctg
    27241 tggtaaaggt acatatatta gaacgctagc aactgatatt ggtgtgaaat taggctttcc
    27301 ggcacatatg tcgaaattaa cacgaatcga gtctggtgga tttgtgttga aagatagcct
    27361 tacattagaa caaataaaag aacttcatga gcaggattca ttgcaaaata aattgtttcc
    27421 tttagaatat ggattaaagg gtttgccaag cattaaaatt aaagattcgc acataaaaaa
    27481 acgtatttta aatgggcaga aatttaataa aaatgaattt gataacaaaa ttaaagacca
    27541 aattgtattt attgatgatg attcagaaaa agtattagca atttatatgg tacaccctac
    27601 aaaagaatca gaaattaaac ctaaaaaagt ctttaattaa aggagataga atttatgaaa
    27661 gtcatagaag tgacacatcc tatacaatca aaacagtata atacagagga tgttgcaatg
    27721 gcattcggat ttttcgatgg catgcataaa ggtcatgaca aagtctttga tatattaaac
    27781 gaaatagctg aggcacgcag tttaaaaaaa gcggtgatga catttgatcc gcatccgtct
    27841 gtcgtgttga atcctaaaag aaaacgaaca acgtatttaa cgccactttc agataaaatc
    27901 gaaaaaatta gccaacatga tattgattat tgtatagtgg ttaatttttc atctaggttt
    27961 gctaatgtga gcgtagaaga ttttgttgaa aattatataa ttaaaaataa tgtaaaagaa
    28021 gtcattgctg gttttgattt tacttttggt aaatttggaa aaggtaatat gactgtactt
    28081 caagaatatg atgcgtttaa tacgacaatt gtgagtaaac aagaaattga aaatgaaaaa
    28141 atttctacaa cttctattcg tcaagattta atcaatggtg agttgcaaaa agcgaatgat
    28201 gctttaggct atatatattc tattaaaggc actgtagtgc aaggtgaaaa aaggggaaga
    28261 actattggct tcccaacagc taacattcaa cctagtgatg attatttgtt acctcgtaaa
    28321 ggtgtttatg ctgttagtat tgaaatcggc actgaaaata aattatatcg aggggtagct
    28381 aacataggtg taaagccaac atttcatgat cctaacaaag cagaagttgt catcgaagtg
    28441 aatatctttg actttgagga taatatttat ggtgaacgag tgaccgtgaa ttggcatcat
    28501 ttcttacgtc ctgagattaa atttgatggt atcgacccat tagttaaaca aatgaacgat
    28561 gataaatcgc gtgctaaata tttattagca gttgattttg gtgatgaagt agcttataat
    28621 atctagagtt gcgtatagtt atataaacaa tctataccac acctttttct tagtaggtcg
    28681 aatctccaac gcctaactcg gattaaggag tattcaaaca ttttaaggag gaaattgatt
    28741 atggcaattt cacaagaacg taaaaacgaa atcattaaag aataccgtgt acacgaaact
    28801 gatactggtt caccagaagt acaaatcgct gtacttactg cagaaatcaa cgcagtaaac
    28861 gaacacttac gtacacacaa aaaagaccac cattcacgtc gtggattatt aaaaatggta
    28921 ggtcgtcgta gacatttatt aaactactta cgtagtaaag atattcaacg ttaccgtgaa
    28981 ttaattaaat cacttggtat ccgtcgttaa tcttaatata acgtctttga ggttggggca
    29041 tatttatgtt ccaaccttaa tttatattaa aaaagctttt tacaaatatt aacatttatt
    29101 atatgttaag ctaatattga
//
LOCUS       DAMZKC010000016        28005 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.016, whole genome shotgun sequence.
ACCESSION   DAMZKC010000016 DAMZKC010000000
VERSION     DAMZKC010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 28005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28005
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.016"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            180..27045
                     /gene="ebh"
                     /locus_tag="POB55_000507"
                     /pseudo
     CDS             180..27045
                     /gene="ebh"
                     /locus_tag="POB55_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001635093.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hyperosmolarity resistance protein Ebh"
     gene            complement(27104..27505)
                     /locus_tag="POB55_000508"
     CDS             complement(27104..27505)
                     /locus_tag="POB55_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HDI6499499.1"
                     /translation="MAKINFDAATKGNPGISTCAIVIKEDEQHYTYTHELGEMDNHTA
                     EWAACIYALEHARELNVQNALLYTDSKIIADSIEAGYVKNANFKPYFDQIEIFEKDFD
                     LLFVKWIPREQNKEANQHAQQALYKLIKKNK"
BASE COUNT        11244 a         4986 c         5071 g         6704 t
ORIGIN      
        1 cacatagttg taatgtgttt caatactttt tggatagatt gcgaaattgt attgaatcgt
       61 catcgtttta aatttttaaa tgagaatgga atgagcatta caatatacaa gcaatcaaaa
      121 gtaaatacat tcacaacaca acagagacat aacaacaaga taaggagtga acaatagctg
      181 tgaattatcg tgataaaatt caaaagttta gtattcgtaa atatacagtt ggtacatttt
      241 caactgtcat tgcgacattg gtatttttag gattcaatac atcacaagca catgctgctg
      301 aaacaaatca accagcaagc gtggttaaac agaaacaaca aagtaataat gaacagactg
      361 agaatcgaga atctcaagta caaaattctc aaaattcaca aaatagtcaa tcattatctg
      421 ctactcatga aaatgagcaa ccaaatatta gtcaagctaa tttagtagat caaaaagtag
      481 cgcaatcatc tactactaat gatgaacaac cagcatctca aaatgtaaat acaaagaaag
      541 attcggcaac ggctgcgaca acacaaccag ataaagaaga aggtaagcat aaacaaaacg
      601 aaagtcaatc tgctaataaa aatggaaacg acaatagagc ggctcattta gaaaatcatg
      661 aagcaaatgt agtaacagct tcagattcat ctgataatgg taacgtacaa catgaccgaa
      721 atgaattaca agcatttttt gatgcaaatt atcatgatta tcgctttatt gaccgtgaaa
      781 atgcagattc tggcacattt aactatgtaa aaggcatttt tgaaaagatt aatacgttat
      841 taggcagtaa tgatccaata aacaataaag acttgcaact tgcatacaaa gaattggaac
      901 aagctgttgc tttaattcgt acaatgcctc aacgtcaaca aactagccgt cgatcaaaca
      961 gaattcaaac gcgttctgtt gagtctagag ctgcagagcc tagatcagta tcagactatc
     1021 aaaatgcaaa ttcatcatat tatgttgaaa atgctaatga tggttcagga tatcctgtag
     1081 gtacatatat caatgcttct agtaaagggg cgccatataa tttaccaact acaccatgga
     1141 atacattgaa ggcctctgac tcaaaggaaa ttgctcttat gacagcgaaa caaactggag
     1201 atggctacca atgggttatt aagtttaata aaggacatgc tccacatcaa aatatgattt
     1261 tctggtttgc attaccagca gaccaagtgc cagtaggaag aactgacttt gtaacagtta
     1321 attcagatgg aacaaatgta caatggagtc atggagcaga agcaggtgca aataaaccac
     1381 ttcaacaaat gtgggaatat ggagtaaatg atcctgatcg ttcacatgac tttaaaataa
     1441 gaaatagaag tggccaagta atatatagct ggccaactgt ccatgtttat tctttagaag
     1501 atttatctag agcgagtgat tattttagtg aagctggagc aacagctgct actaaagcat
     1561 ttggtagaca aaattttgaa tatattaatg gtcaaaaacc tgctgaatca ccgggtgttc
     1621 ctaaagttta tactttcatc ggtcaaggtg atgcaagtta tacaatttca tttaaaacac
     1681 aaggtccaac tgttaataaa ttgtactatg cagcaggtgg gcgtgcttta gagtacaatc
     1741 aattatttat gtacagtcaa ctatacgtcg aatcaacgca agaccatcaa caacgtctta
     1801 atggtttaag acaagtggtt aatcgtacat atcgcatagg tacaactaaa cgtgtagaag
     1861 tgagtcaagg aaatgtacaa acgaaaaagg tattagaaag tacaaaccta aatatagatg
     1921 attttgttga tgatccatta agttatgtta agacgccgag taataaagtg ttaggttttt
     1981 acccaactaa tgcaactact aacgctttta gaccgggggg cgttcaggaa ttaaatgaat
     2041 atcaattaag tcaattattt actgatcaaa aattacaaga agcagcaaga actagaaacc
     2101 caataagatt aatgattggt ttcgactatc ctgatggtta tggtaatagt gaaactttag
     2161 ttcctgttaa cttaacggta ttacctgaaa tccaacataa tattaaattc tttaaaaatg
     2221 acgatactca aaacattgct gaaaaaccat tttcaaaaca agctgggcat ccagttttct
     2281 atgtatatgc aggtaaccaa gggaatgctt ccgtgaattt aggtggtagc gtaacatcta
     2341 ttcaaccatt acgtattaat ttaacaagta atgagaattt tacagataaa gattggcaaa
     2401 ttacaggtat tccgcgtaca ttacacattg aaaactcgac aaatagaact aataatgcta
     2461 gagaacgtaa cattgaactt gttggtaatt tattaccagg ggattacttt ggaacgatac
     2521 gttttggacg taaagaacaa ttattcgaaa ttcgtgttaa accacataca ccaacaatta
     2581 caacgacagc tgagcaatta agaggtacag cattacaaaa agtgcctgtt aatatttcgg
     2641 gaataccgtt ggatccatcg gcattggttt atttagttgc accaacaaat caaactacga
     2701 atggtggtag tgaggcagat caaataccat ctggttatac gatacttgcg actggtacac
     2761 ctgatggggt gcataataca attactatac gaccgcaaga ttatgttgta ttcataccac
     2821 ctgtaggtaa acaaattaga gcagtagttt attataataa agtagttgca tctaatatga
     2881 gtaatgctgt tactattttg ccagatgaca ttccaccaac aatcaataat cctgttggaa
     2941 taaatgccaa atactatcga ggcgacgaag tcaactttac aatgggagtc tctgatagac
     3001 attctggtat aaaaaataca actattacta ctttgccgaa tggttggaca tcaaatttaa
     3061 ctaaatccga caacaaaaac ggctcattag ctattacagg tagagtgagt atgaatcagg
     3121 cgtttaacag tgatattaca tttaaagtat cagcgacaga taatgtcaat aatacgacaa
     3181 atgatagtca atctaaacat gtgtcaattc atgtaggtaa aattagtgaa gatgctcatc
     3241 cgattgtatt aggaaatact gagaaagttg tagtagtcaa tccgactgct gtatctaatg
     3301 atgaaaagca aagcataatt actgccttta tgaataaaaa ccaaaatata agaggatatt
     3361 tagcatcaac tgatccagta actgtcgata ataatggtaa tgtcacatta cattaccgtg
     3421 atggctcatc aacaacgctt gatgctacaa atgtgatgac atacgaacca gttgtgaaat
     3481 ctgaatatca aactgccaat gcagctaaaa cagcaacggt aacgattgct aaaggacaat
     3541 catttaatat tggtgatatt aaacaatatt ttactttaag taatggacaa gctattccaa
     3601 gtggcacatt tacaaatatt acatctgata gaactattct aactgcacaa gaagttagtc
     3661 aaatgaatgc aggcacgcag ttatatcata tagttgcttc aaatgcatat cataaagaca
     3721 ctgaagattt ctatattagt ttaaaaatcg ttgatgtgaa acaacctgaa ggcgatcaac
     3781 gtgtctatcg tacgtcaaca tatgatttaa caactgatga aatctcaaaa gtaaaacaag
     3841 cttttattaa tgcaaataga gatgtaatta cgcttgccga gggtaatatt tcagttacaa
     3901 atacacctaa tggtgctaat gtaagtacta ttacagtaaa tattaataaa ggtcgattaa
     3961 cgaaatcatt cgcgtcaaac ctagctaata tgaatttctt gcgttgggtt aatttcccgc
     4021 aagattatac agtgacatgg acgaatgcaa aaattgcaaa cagaccaaca gatggtggtt
     4081 tatcatggtc tgatgaccat aaatctttaa tttatcgtta tgatgctaca ttaggcacac
     4141 aaattacaac taatgatatt ttaacaatgc taaaagcgac tactacagtg cctggattgc
     4201 gtaataatat tactggtaat gaaaaagcac aagcagaagc tggcggaaga cctaacttta
     4261 gaacgactgg ttattcacaa tcaaatgcga caactgatgg tcaacgtcaa tttacgttga
     4321 atggtcaagt gattcaagtg ttagacatca tcaacccttc aaacggttat ggtgggcaac
     4381 ctgttacaaa ttcaaatact cgtgcaaacc atagtaactc aactgttgtt aacgtaaacg
     4441 aaccggcagc taatggtgct agcgcattta caattgacca cgttgtaaaa agtaattcta
     4501 cacataatgc aagtgatgca gtttataaag cacagttata cttaacgcca tatggtccaa
     4561 aacaatatgt tgaacattta aatcaaaata caggaaatac tactgacgct attaacattt
     4621 attttgtacc aagtgattta gtgaatccaa caatttcagt aggtaattac actaatcatc
     4681 aagtgttctc aggtgaaaca tttacaaata ctattacagc gaatgataac tttggtgtgc
     4741 aatctgtaac tgtaccaaat acatcacaaa ttacaggcac tgttgataat aaccatcaac
     4801 atgtttctgc aacggcacca aatgtgacat cagcaactaa taagacaatc aatttattag
     4861 caactgatac aagcggcaat acagctacaa cttcgttcaa tgtaacagtg aaacctttgc
     4921 gtgataaata tcgagttggt acttcatcaa cggctgctaa tcctgtgaga attgccaata
     4981 tttcgaataa tgcgacagta tcacaagctg atcaaacagc aattattaat tcattaacgt
     5041 ttactgaaac agtaccaaat agaagttatg caagagcaag tgcgaatgaa atcactagta
     5101 aaacagttag taatgtcagt cgtactggaa ataatgccaa tgttacagta actgctactt
     5161 atcaagatgg aacaacatca acagtgactg tacctgtaaa gcatgtcatt ccagaaatcg
     5221 ttgcacattc gcattacact gtacaaggcc aagacttccc agcaggtaat ggttctagtg
     5281 catcagatta ctttaagtta tctaatggta gtgccattcc agatgcaact attacatggg
     5341 taagtggaca agcgccaaat aaagataata cacgtattgg tgaagatata acagtaactg
     5401 cacatatctt aattgatggt gaaacaacgc cgattacgaa aacagcaaca tataaagtag
     5461 taagtacagt accgaagcat gtctttgaga cgaatagagg tgctgtgttc ccaggtgtat
     5521 ctgatgtgta tgatgcgaaa caatatgtta aaccagttaa tgattcttgg acacaaaatg
     5581 cgcaacgtat gaatttccaa tttacaaatt catatggacc aagtaaagat gtggttggta
     5641 tttcaacaag ggatattcga gttacttatg ataatcacca aacgcaaatt atcaagattt
     5701 tagcaaaagt taaaccagat ccaccgcgaa ttgatggcaa ctctgtgact tataaagcag
     5761 gtcttactaa ccaacaaatt aaaattaata atgtgttaag tagttcatct attaaattat
     5821 ttaaagcaga taatacgcca ttaacgatta caaacacgac ttatggtagt ggtaatactg
     5881 cagttgtaac ggtaagtgat gcgttaccaa atggtgtgat taaggcaagg tcttctatca
     5941 caatgaataa tgtgacgtac actactcaag atgaacatgg tcgagcgata gatgtaacaa
     6001 gaaatgaatc tgttgattca aatgacagtg caacagtaac agtgacacca caattacaag
     6061 caactactga aggtgctgta tttattaaag gtggcgacgg ttttgatttc ggacacgtag
     6121 aaagatttat tcaaaacccg ccacatgggg caacggttgc atggcatgat agtccagata
     6181 catggaagaa tacagtcggt aacactcata aaactgcggt tgtaacatta cctaatggtc
     6241 aaggtacgcg taatgttgaa gttccagtca aagtttatcc agttgctaat gcaaaggcgc
     6301 catcacgtga tgtgaaaggt caaaatttga ctaatggaac ggatgcgatg aactacatta
     6361 catttgatcc aaatacaaac acaaatggta tcactgcagc atgggcaaat agacaacaac
     6421 caaataacca acaagcaggc gtgcaacatt taaatgtcga tgtcacatat ccaggtattt
     6481 cagctgctaa acgagttcct gttactgtta atgtatatca atttgaattc cctcaaacta
     6541 cttatacgac aacggttgga ggcactttag caagtggtac gcaagcatca ggatatgcac
     6601 atatgcaaaa tgctactggt ttaccaacag atggatttac gtataaatgg aatcgtgata
     6661 ctacaggtac aaatgacgca aactggtcag ctatgaataa accgaatgtg gctaaagtcg
     6721 ttaacgcaaa atatgacgtc atctataacg gacatacttt tgcaacatct ttaccagcga
     6781 aatttgtagt aaaagatgtg caaccagcga aaccaactgt gactgaaaca gcggcaggag
     6841 cgattacaat tgcacctgga gcaaaccaaa cagtgaatac acatgcaggt aacgtaacga
     6901 catacgctga taaattagtt attaaacgta atggtaacgt tgtgacgaca tttacacgtc
     6961 gcaataatac aagtccatgg gtgaaagaag catctgcagc aactgtagca ggtattgctg
     7021 gaactaataa tggtattact gttgcagcag gtactttcaa ccctgctgat acaattcaag
     7081 ttgttgcaac gcaaggaagc ggagagacag taagtgatga gcaacgtagt gatgatttca
     7141 cagttgtcgc accacaaccg aaccaagcga ctactaagat ttggcaaaat ggtcatattg
     7201 atatcacgcc taataatcca tcaggacatt taattaatcc aactcaagca atggatattg
     7261 cttacactga aaaagtgggt aatggtgcag aacatagtaa gacaattaat gttgttcgtg
     7321 gtcaaaataa tcaatggaca attgcgaata agcctgacta tgtaacgtta gatgcacaaa
     7381 ctggtaaagt gacgttcaat gccaatacta taaaaccaaa ttcatcaatc acaattactc
     7441 cgaaagcagg tacaggtcac tcagtaagta gtaatccaag tacattaact gcaccggcag
     7501 ctcatactgt caacacaact gaaattgtga aagattatgg ttcaaatgta acagcagctg
     7561 aaattaacaa tgcagttcaa gttgctaata aacgtactgc aacgattaaa aatggcacag
     7621 caatgcctac taatttagct ggtggtagca caacgacgat tcctgtgaca gtaacttaca
     7681 atgatggtag tactgaagaa gtacaagagt ccattttcac aaaagcggat aaacgtgagt
     7741 taatcacagc taaaaatcat ttagatgatc cagtaagcac tgaaggtaaa aagccaggta
     7801 caattacgca gtacaataat gcaattcata atgcgcaaca acaaatcaat actgcaaaaa
     7861 cagaagcgca acaagtgatt aataatgatc gtgcaacacc acaacaagtt tctgacgcat
     7921 taactaaagt tcgtgcagca caaactaaga ttgatcaagc taaagcatta cttcaaaata
     7981 aagaagataa tagccaatta gtaacatcta aaaataactt acaaagttct gtgaaccaag
     8041 taccatcaac tgctggtatg acgcaacaaa gtattgataa ctataatgcg aagaagcgtg
     8101 aagcagaaac tgaaataact gcagcacaac gtgttataga caatggagat gcaacaccgc
     8161 agcaaatttc agaggaaaaa catcgtgtcg ataacgcatt aacagcatta aaccaagcga
     8221 aacaaaattt aactacagat acacatacct tagagcaagc agtgcaacaa ttgaatcgca
     8281 caggtacaac gactggtaag aaaccagcaa gtattactgc gtataataat tcaatgcatg
     8341 cgcttcaagc agaacttaca agtgctaaaa atagtgctaa tgctatcatt cagaagccaa
     8401 taagatcagt gcaagaggta caaacagcat taacaaatgt aaatcgtgtt aatgagcgat
     8461 taacgcaagc aattaatcaa ttagtacctt tagctgataa tagtgcttta agaactgcta
     8521 agacgaaact tgatgaagaa atcaataaat cagtaactac tgatggtatg acacaatcat
     8581 caatccaagc atatgaaaat gctaaacgtg cgggtcaaac agaatcaaca aatgcacaaa
     8641 atgttattaa caatggtgac gcaacagacc aacaaattgc cgaagaaaaa acaaaagtag
     8701 aagaaaaata taatagctta aaacaagcaa ttgctggatt aacaccagac ttggcaccat
     8761 tacaaactgc aaaaactcag ttgcaaaatg atattgatca gccaacgagt acgactggta
     8821 tgacaagcac atctattgca gcatttaatg aaaaactttc agcagctaga actaaaattc
     8881 aagaaattga tcgcgtactg gcatctcatc cagatgttgc aacgattcgt caaaacgtga
     8941 cagcagcgaa tgctgctaaa acagcacttg atcaagcacg caatggctta acagttgata
     9001 aagcgccttt agaaaatgcg aaaaatcaac tacaacatag tattgacacg caaacaagta
     9061 caactggtat gacacaagac tctataaatg catacaatgc gaagttaaca gctgcacgta
     9121 ataagattca acaaatcaat caagtattag caggttcacc gactgtagaa caaattaata
     9181 caaatacgtc tgcagcaaat caagctaaat ctgatttaga tcatgcacgt caagcgttaa
     9241 caccagataa agcaccgctt caaactgcga aaacgcaatt agaacaaagc attaatcaac
     9301 caacggatac aacaggtatg acgaccgctt cgttaaatgc gtacaaccaa aaattacaag
     9361 cagcgcgtca aaagttaact gaaattaatc aagtgttgaa tggcaaccca actgtccaaa
     9421 atatcaatga taaagtgaca gaggcaaacc aagctaagga tcaattaaat acagcacgtc
     9481 aaggtttaac attagataga cagccagcgt taacaacatt acatggtgca tctaacttaa
     9541 accaagcaca acaaaataat ttcacgcaac aaattaatgc tgctcaaaat catgctgctc
     9601 ttgaaacaat taagtctaac attacggctt taaatactgc gatgacgaaa ttaaaagaca
     9661 gtgttgcgga taataataca attaaatcag gtcaaaatta cactgacgca acaccagcta
     9721 ataaacaagc gtatgataat gcagttaatg cggctaaagg tgtcattgga gaaacgacta
     9781 atccaacgat ggatgttaac acagtgaacc aaaaagcagc atctgttaaa tcgacgaaag
     9841 atgctttaga tggtcaacaa aacttacaac gtgcgaaaac agaagcaaca aatgcgatta
     9901 cgcatgcaag tgatttaaac caagcacaaa agaatgcatt aacacaacaa gtgaatagtg
     9961 cacaaaatgt acatgcagta aatgatatta aacaaacgac tcaaagctta aatactgcta
    10021 tgacaggttt aaaacgtggc gttgctaatc ataatcaagt cgtacaaagt gataattatg
    10081 tcaacgcaga tactaataag aaaaatgatt acaacaatgc atacaaccat gcgaatgaca
    10141 ttattaatgg taatgcacaa catccagtta taacaccaag tgatgttaac aatgctttat
    10201 caaatgtcac tagtaaagaa catgcattga atggtgaagc taagttaaat gctgcgaaac
    10261 aagaagcgaa cactgcatta ggtcaattaa ataacttgaa taatgcgcaa cgtcaaaact
    10321 tacaatcgca aattaatagt gcacatcaaa tcgagacagt taatacgatt aagcaaaatg
    10381 caacaaactt aaatagtgca atgggtaact taagacaagc tgttgcagat aaagatcaag
    10441 tgaaacgtac agaagattat gcggatgcag atacagctaa acaaaatgca tataacagtg
    10501 cagtttcaag tgccgaaaca atcattaatc aaacaacaaa tccaacgatg tctgttgatg
    10561 atgttaatcg tgcaacttca gctgttactt ctaataaaaa tgcattaaat ggtgatgaaa
    10621 aattagcaca atctaaaaca gatgctgcaa gagcaattga tgcattacca catttaaata
    10681 atgcacaaaa agcagatgtt aaatctaaaa ttaatgctgc atcaaatatt gctggcgtaa
    10741 atactgttaa acaacaaggt acagatttaa atacagcgat gggtaacttg caaggtgcaa
    10801 tcaatgatga acaaacgacg cttaatagtc aaaactatca agatgcgaca cctagtaaga
    10861 aaacagcata cacaaatgcg gtacaagctg cgaaagatat tttaaataaa tcaaatggtc
    10921 aaaataaaac gaaagatcaa gttactgaag cgatgaatca attgaattcg gctaaaaata
    10981 acttagatgg tacgcgttta ttagatcaag cgaagcaaac agcgaaacag cagttaaata
    11041 atatgacgca tttaacaact gcacaaaaaa cgaatttaac aaaccaaatt aatagtggta
    11101 ctactgtcgc tggtgttcat acggttcaat caaatgccaa tacattagat caagccatga
    11161 atacgttaag acaaagtatt gccaacaaag atgcgactaa agcaagtgaa gattacgtag
    11221 atgctaataa tgataagcaa acagcatata acaacgcagt agctgctgct gaaacgatta
    11281 ttaatgcaaa tagtaatcca gaaatgaatc caagtacgat tacacaaaaa gcagagcaag
    11341 tgaatagttc taaaacggca cttaacggtg atgaaaactt agctacggca aaacaaaatg
    11401 cgaaaacgta cttaaacaca ttaacgagta ttacagatgc tcaaaagaac aatttgatta
    11461 gtcaaattag tagtgcgaca agagttagtg gtgttgatac tgtaaaacaa aatgcacaac
    11521 atctagatca agctatggct agcttgcaaa gtggtattaa caacgaatct caagttaaat
    11581 catctgagaa atatcgtgat gctgatacaa ataaacaaca agagtatgat aatgctatta
    11641 ctgcagcgaa agcgatttta aataaacaac atggtccaaa cactgcgcaa aatgcagttg
    11701 aagcagcatt gcaacgtgtg aatactgcga aagatgcatt gaatggcgat gcaaaattaa
    11761 ttgcagctca aaacgcagcg aaacaacatt taggtacttt aacgcatatc actacagctc
    11821 aacgtaatga tttaacaaat caaatttcac aagctacaaa cttagctggt gttgaatctg
    11881 ttaaacaaaa tgcgaatagt ttagatggtg ctatgggtaa cttacaaacg gctatcaacg
    11941 ataagtcagg aacattagcg agccaaaact tcttggatgc tgatgagcaa aaacgtaatg
    12001 catacaatca agctgtatca gcagccgaaa ccattttaaa taaacaaact ggaccgaata
    12061 cagcgaaaac agcagtcgaa caagcactta ataatgttaa taatgcgaaa catgcattaa
    12121 atggtacgca aaacttaaac aatgcgaaac aagcagcgat tacagcaatc aatggcgcat
    12181 ctgatttaaa tcaacatcaa aaagatgcat taaaagcaca agctaatggt gctcaacgcg
    12241 tatctaatgc acaagatgta caacgtaatg cgactgaact gaacacggcg atgggcacat
    12301 taaaacatgc catcgcagat aagacgaata cgttagcaag cagtaaatat gtcaacgccg
    12361 atagcactaa acaaaatgct tacacaacta aagttaccaa tgctgaacat attattagcg
    12421 gtacgccaac ggttgttacg acaccttcag aagtaacagc tgcagctaat caagtaaaca
    12481 gcgcgaaaca agaattaaat ggtgacgaaa gattacgtgt agcaaaacaa aacgccaata
    12541 ctgctattga tgcattaacg caattaaata ctcctcaaaa agctaaatta aaagaacaag
    12601 tgggacaagc caatagatta gaagacgtac aatctgttca aacaaatgga caagcattga
    12661 acaatgcaat gaaaggctta agagatagta ttgctaacga aacaacagtc aaagcaagtc
    12721 aaaactatac agacgcaagt ccgaataacc aatcaacata taatagcgct gtgtcaaatg
    12781 cgaaaggtat cattaatcaa actaacaatc cgactatgga tgctagtgcg attacccaag
    12841 ctgcaacaca agtgaataat gctaaaaatg gtttaaacgg tgctgaaaac ttaagaaatg
    12901 cacaaaacac tgctaagcaa aacttaaata cgttatcaca cttaacaaat aaccaaaaat
    12961 ctgccatctc atcacaaatt gaccgtgcag gtcatgtgag tgaggtaaca gctgctaaaa
    13021 atgcagcaac tgagttaaac acgcaaatgg gcaacttgga acaagctatc catgatcaaa
    13081 acacagttaa acaaggtgtt aacttcactg atgcagataa agctaaacgt gatgcgtata
    13141 caaatgcggt aagcagagct gaagcaattc tgaataaaac gcaaggtgca aatacgtcta
    13201 aacaagatgt tgaagcggct attcaaaatg tttcaagtgc taaaaatgca ttgaatggtg
    13261 atcaaaacgt tacaaatgcg aagaatgcag ctaaaaatgc attaaataac ttaacgtcaa
    13321 ttaataatgc acaaaaacgt gacttaacaa ctaaaattga tcaagcaaca actgtagctg
    13381 gtgttgaagc tgtatctaat acaggtacac aattgaatac agcgatggct aacttgcaaa
    13441 atggtattaa tgataaagca aatacactag caagtgaaaa ctatcatgat gctgattcag
    13501 ataagaaaac tgcttatact caagccgttg cgaacgcaga aaatatttta aataaaaata
    13561 gtggatcaaa tttagataaa gctgccgttg aaaacgcgtt gtcacaagtt gctaatgcga
    13621 aaggtgccct aaatggtaac cataatttag agcaagctaa atcaaatgca aacactacta
    13681 taaacggact tcaacattta acaactgctc aaaaagataa attgaaacaa caagtgcaac
    13741 aagcacaaaa tgttgcaggt gtagatactg ttaaatcaag tgccaacaca ttaaatggtg
    13801 ctatgggtac gttaagaaat agcattcaag ataacacagc tacgaaaaat ggccaaaact
    13861 atcttgatgc tacaggaagt aacaaaacaa actataacaa tgctgttgat agtgctaatg
    13921 gtgtcattaa tgcaacaagc aatccaaata tggatgctaa tgcaattaac caaatcgcta
    13981 cacaagtgac atcaacgaaa aatgcattag atggtacaca taatttaacg caagcgaaac
    14041 aaacagcaac aaatgccatc gatggtgcta ctaacttaaa taaagcgcaa aaagatgcgt
    14101 tgaaagcaca agtgacaagt gcgcaacgtg ttgcaaatgt aacaagtatc caacaaactg
    14161 caaatgaact taatacagct atgggtcaat tacaacatgg tattgatgat gaaaatgcaa
    14221 caaaacaaac tcaaaaatat cgtgacgctg aacaaagtaa gaaaactgct tatgatcaag
    14281 ctgtagctgc tgcgaaagca attttaaata aacaaacagg ttcaaattca gataaagcag
    14341 cagttgaccg tgcattacaa caagtaacaa gtacgaaaga tgcattgaat ggtgatgcaa
    14401 aactggcaga agcgaaagcg gcagctaaac aaaacttagg cactttaaac catattacga
    14461 atgcacaacg tactgcgtta gaaggtcaaa tcaatcaagc gacgactgtt gatggcgtta
    14521 atactgtaaa aacaaatgcc aatacattag acgacgcaat gaatagctta caaggtgcaa
    14581 tcaatgataa agatgctaca ttaagaaatc aaaattatct tgatgcagat gaatcaaaac
    14641 gaaatgcata tacgcaagct gtcacagcgg ctgaaggcat tttaaataaa caaactggtg
    14701 gtaacacatc taaagcagac gttgataatg cattaaatgc agttacaaga gcgaaagcgg
    14761 ctttaaatgg tgctgaaaac ttaagaaatg cgaaaacttc agcaacaaat acgattaatg
    14821 gtttacctaa cttaacacaa ttacaaaaag acaacttgaa gcatcaagtt gaacaagcgc
    14881 aaaatgtagc aggtgtaaat ggtgttaaag ataaaggtaa taccttaaat actgccatgg
    14941 gtgcattacg tacaagtatc caaaatgata atacgacgaa aacaagtcaa aattatcttg
    15001 atgcatctga tagcaacaaa aataattaca atactgctgt aaataatgca aatggtgtta
    15061 ttaatgcaac gaacaatcca aatatggatg ctaatgcgat taatggcatg gcaaatcaag
    15121 tcaatacaac aaaagcagcg ttaaatggtg tacaaaactt agctcaagct aaaacaaatg
    15181 cgacgaacac aattaacaac gcacatgact taaaccaaaa acaaaaagat gcattaaaaa
    15241 cacaagttaa caatgcacaa cgtgtatctg atgcaaataa cgttcaacat acagctactg
    15301 aattgaacgg tgcgatgaca gcacttaaag cagctattgc tgataaagaa agaacaaaag
    15361 caagcggtaa ttatgtcaat gctgatcaag aaaaacgtca agcgtatgat tcaaaagtga
    15421 ctaacgctga aaatatcatt aatggtacac cgaatgcgac attaacagtc aatgatgtaa
    15481 atagtgcgac ttcacaagtc aatgcggcta aaacagcatt aaatggcgat aacaacttac
    15541 gtgttgctaa agaaaatgct aataatacaa ttgatggctt agcacaattg aataatgcac
    15601 aaaaagcaaa attaaaagaa caagttcaaa gtgcaactac attagaaggt gttcaaactg
    15661 ttaaaaatag ttctcaaacg ttgaatacag cgatgaaagg cttaagagat agtattgcga
    15721 atgaagcaac aattaaagca ggtcaaaact acactgacgc aagtccaact aatcgtaacg
    15781 agtacgacag cgcagttact gcagcaaaag caatcattaa tcaaacatcg aacccaacga
    15841 tggaaccaaa tactattacg caagcaacat cacaagtgac aactaaagaa catgcattaa
    15901 atggtgcgca aaacttagct caagctaaga caactgcgaa aaacaactta aataacttaa
    15961 catcaattaa caatgcacaa aaagatgcgt taacgcgtag cattgatggt gcaactacag
    16021 tagctggtgt aaatcaagaa actgccaaag caacagaatt aaataacgca atgcatagtt
    16081 tacaaaatgg tatcaatgat gagacacaaa caaaacaaac tcagaaatac ctagatgcag
    16141 agccaaataa gaaatcagct tatgatcaag ctgtaaatgc agcgaaagca attttaacaa
    16201 aagctagtgg tcaaaatgta gacaaagcag cagttgaaca agcattacaa aatgtgaaca
    16261 gtacgaagac ggcgttgaac ggtgatgcga aattaaatga agctaaagct gctgcgaaac
    16321 aaacgttagg tacattaaca cacattaata atgcacaacg taatgcgtta gataatgaaa
    16381 ttacacaagc aacaaatgtt gaaggtgtta atacagttaa agccaaagcg caacaattag
    16441 atggtgctat gggtcaatta gaaacatcaa ttcgtgataa agacacgacg ttacaaagtc
    16501 aaaattatca agatgctgat gatgctaaac gaacggctta ttctcaagca gtaaatgcag
    16561 cagcaactat tttaaataaa acagctggcg gtaatacacc taaagcagat gttgaaagag
    16621 caatgcaagc tgttgcacaa gcaaatactg cattaaacgg tattcaaaac ttagaacgtg
    16681 cgaaacaggc tgctaacaca gcgattacaa acgcttcgga cttaaataca aaacaaaaag
    16741 aagcattaaa agcacaagta acaagtgcag gacgcgtatc tgcagcaaat ggtgttgaac
    16801 atactgcgac tgaaataaat actgcgatga cagctttaaa acgtgctatt gctgataaag
    16861 ctgacacaaa aactagtggt aactatgtca atgctgatgc gaataaacgt caagcatatg
    16921 atgaaaaagt gacagctgcc gaaagtatcg ttaatggtac accaacacca acgttaacac
    16981 catcagatgt tacaaatgca gcaacgcaag taacgaatgc gaagacgcag ttaaacggta
    17041 atcataattt agaagtagcg aaacaaaatg ctaacacagc aattgatggt ttaacttctt
    17101 taaatggtcc gcaaaaagca aaacttaaag aacaagtggg tcaagcgacg acgttgccaa
    17161 atgttcaaac tgttcgtgat aatgcacaaa cattaaacac tgcaatgaaa ggtctacgag
    17221 atagcattgc gaatgaagca acgattaaag caggtcaaaa ctacacagat gcaagtccta
    17281 ataatcgtag tgaatacgat agtgcagtca ctgcagcaaa agcaatcatt ggtcaaacaa
    17341 ctagtccatc aatgaatgcg caagaaatta atcaagcgaa agaccaagtg acagctaaac
    17401 aacaagcgtt aaacggtcaa gaaaacttaa gaactgcgca aacaaatgcg aagcaacatt
    17461 tgaatggctt aagtgactta actaatgctc aaaaagaagc ggcgaaacgt caaatcgagg
    17521 gtgcaacgca tgttaatgaa gtaacacaag cacaaaataa tgcggatgca ttaaatacag
    17581 ctatgacgaa cttgaaaaat ggtattcaag atcagaatac gattaagcaa ggtgttaact
    17641 tcactgatgc agatgaagcg aaacgtaatg catatacaaa tgcagtgacg caagctgaac
    17701 aaattttaaa taaagcacaa ggtccaaata ctgcaaaaga caatgttgaa tctgcgttac
    17761 aaaatgtaca acgtgctaaa aacgaattga acggtaatca aaatgttgcg aatgctaaga
    17821 caactgcgaa aaatgcattg aataacttaa catcaatcaa caacgcacaa aaagaagcgt
    17881 tgaaatcaca aattgaaggt gctacaacag ttgcaggtgt aaatcaagtg tctacaacag
    17941 catctgaatt aaatactgca atgagcaact tacaacgtgg tattaacgat gaagcagcta
    18001 caaaagcagc tcagaaatat actgatgcag atagagataa acaaactgca tacaatgatg
    18061 ctgtaacggc agctaaaacg ttattagata aaacagctgg cacaaatgaa aataaagcag
    18121 ccgttgaaca agcattacaa cgtgtgaata ctgctaaaac tgcattaaat ggtgatgcgc
    18181 gcttaaatga agcgaagaac acagctaaac aacaagtagc gacaatgtca cacttaactg
    18241 atgctcaaaa agcaaactta acatcgcaaa tcgaaagtgg tacgactgtt gcaggtgttc
    18301 aaggtattca agctaatgcc ggtactttag atcaagcaat gaatcaatta agacaaagta
    18361 ttgcttctaa agatgcgact aaatcaagcg aagattatca agacgcgaat gcagatttac
    18421 aaaatgcata caatgatgcg gtaactaatg ctgaaggtat tattagtgca acgaataacc
    18481 ctgaaatgaa tcctgataca attaaccaaa aagcgagcca agtgaacagt gcgaagtctg
    18541 cattgaacgg tgatgaaaaa ttagcagcag caaaacaaac tgcgaaatca gatatcggtc
    18601 gtttgacaga cttgaacaat gcacaacgaa ctgcggcaaa tgctgaagtg gatcaagcac
    18661 caaatcttgc agctgttaca gcggctaaaa ataaagcaac atcgttaaac acagcgatgg
    18721 gtaatttgaa acatgcactt gctgaaaagg ataatacgaa acgtagtgtc aattacacag
    18781 atgcggatca accaaaacaa caagcgtatg atactgcggt tacacaagca gaagcaatta
    18841 ctaatgctaa tggcagcaac gcgaatgaaa caacagttca agcagcacta aaccaattga
    18901 atcaagctaa aaatgacttg aatggtgata ataaagttgc tcaagcaaaa gagtcagcga
    18961 aacgtgcatt agcttcatat agtaacttga ataatgcgta atcaactgca gcaactagtc
    19021 aaattgacaa tgcaacgaca gtagcaggtg taactgctgc acaaaatact gctaatgaat
    19081 taaatacagc aatgggtcaa cttcaaaatg gtattaatga ccaaaacact gttaaacaac
    19141 aagtgaactt tacagatgct gaccaaggta agaaagatgc ttacacaaat gctgttacga
    19201 atgctcaagg tattttagat aaagcaaacg gtcaaaatat gacaaaagca caagttgaag
    19261 ctgcattaaa tcaagtaacg actgctaaga atgctttaaa cggtgatgca aatgtaagac
    19321 aagcaaaatc agatgcgaaa gcaaatttag gtacattaac acacttaaat aatgcacaaa
    19381 aacaagattt aacatcacaa atcgaaggtg caacaacagt taacggtgta aatagtgtta
    19441 aaacgaaagc acaagactta gatggtgcaa tgcaacgatt agagtcagca atcgcaaata
    19501 aagatcaaac taaagcgagc gaaaactaca tcgacgcaga tccaactaag aaaacagcat
    19561 ttgataatgc tatcacacaa gctgaatctt acttaaataa agatcatggt acgaataaag
    19621 ataagcaagc tgttgaacaa gcaattcaaa gtgtaacgtc tactgaaaat gctttgaacg
    19681 gtgacgcgaa cttacaacgc gctaaaactg aagcaacaca agctatcgat aacttgacac
    19741 atttgaatac accacaaaaa acagcattaa aacaacaagt gaacgctgcg caacgtgtat
    19801 caggtgtaac tgatctgaaa aatagtgcta catcacttga taatgcgatg gatcaattaa
    19861 aacaaggaat tgctgatcat gacacaattg ttgctggtgg taattacact aacgcaagtc
    19921 ctgataagca aggtgcttat actgatgcat ataatgctgc gaaaaacatt gtaaatggtt
    19981 cacctaatgt gattacaaat gcagcagatg ttactgcggc aacacaacgt gtcaataatg
    20041 ctgaaacaag tttaaatggt gattcaaact tagcaactgc gaagcaacaa gctaaagatg
    20101 cattacgtca aatgacacat ttatctgatg cacaaaaaca aagtattact ggtcaaattg
    20161 atagcgcgac acaagtaact ggtgtacaaa gtgtgaaaga caatgcaaca aatcttgaca
    20221 atgcaatgaa tcaacttcga aatagtattg cgaataaaga tgaagtaaaa gcgagtcaac
    20281 catatgttga tgcagataca gataaacaaa atgcatacaa tacagcagtt acaagtgctg
    20341 aaaatatcat taatgcaacg agtcagccaa cacttgatcc atctgcagta acacaagcag
    20401 ctaatcaagt gaacactaac aaaactgcgc ttaatggtgc gcaaaactta gcaaataaaa
    20461 agcaagaaac aactgctaac atcaaccaat taagtcattt aaataatgct caaaagcaag
    20521 atttaaatac acaagtgaca aatgcaccaa atattagcac agtaaatcaa gtgaaaacta
    20581 aagctgaaca attagatcaa gcaatggaac gtttaatcaa cggaatccaa gacaaagatc
    20641 aagtgaaaca aagtgttaac tttacagatg cagatccaga aaaacaaaca gcatacaaca
    20701 atgcggtaac tgctgctgaa aatattatta atcaagcaaa tggtacaaat gcgaaccaat
    20761 cacaagttga agcagcactt tcaactgtaa caactactaa acaagcgttg aatggtgata
    20821 gaaaagtaac agatgctaaa aacaatgcaa accaaacatt atctacgtta gataacttaa
    20881 acaatgcaca aaaaggtgct gttactggaa acatcaatca agcgcacact gtagctgaag
    20941 taacgcaagc cattcaaacc gctcaggaac tgaatacagc gatgggtaac ttgaaaaata
    21001 gcttgaatga taaagacact acacttggca gtcaaaactt tgcagatgca gatccagaga
    21061 agaaaaatgc atacaatgaa gcggttcgta atgctgaaaa tattttaaat aaatctacag
    21121 gtacaaacgt gcctaaagat caagttgaag cagctatgaa tcaagtgaat actacaaaag
    21181 cagcgcttaa tggtactcaa aaccttgaaa aagctaaaca acacgcaaat acagcaattg
    21241 acggtttaag ccatttaaca aatgcacaaa aagaggcatt aaaacaattg gtacaacaat
    21301 cgactactgt tgcagaagca caaggtaatg aacaaaaagc aaacaatgtt gatgcagcaa
    21361 tggacaaatt acgtcaaagt attgcagata atgcgacaac aaaacaaaac caaaattata
    21421 ctgattaata atgcaacaac tcgtgataaa gtggctgaaa tcattgcaca agcgcaagca
    21481 ttaaatgaag cgataaaagc attaaaagaa agtattaagg atcaaccaca aactgaagca
    21541 agtagtaaat ttattaacga ggatcaagcg caaaaagatg cttatacgca agcagtacaa
    21601 cacgcgaaag atttgattaa caaaacaact gatcctacat tagccaaatc aatcattgat
    21661 caagcgacac aggcagtgac tgatgctaaa aacaatttac atggtgatca aaaactagct
    21721 caagataagc aacgtgcaac agaaacgtta aataacttgt ctaacttgaa tacaccacaa
    21781 cgtcaagcac ttgaaaatca aatcaataat gcagcaactc gtgtcgaagt agcacaaaaa
    21841 ttaactgaag cacaagcact taaccaagca atggaagctt tacgtaatag cattcaagat
    21901 caacagcaaa cggaagcggg tagcaagttt atcaatgaag ataaaccaca aaaagatgct
    21961 taccaagcag cagttcaaaa tgcaaaagat ttaattaatc aaactaacaa tccaacgctt
    22021 gataaagcac aagttgaaca attgacacaa gctgttaacc aagctaaaga taacctacac
    22081 ggtgatcaaa aacttgcaga cgataaacaa catgcggtta ctgatttaaa tcaattaaat
    22141 ggtttgaata atccgcaacg tcaagcactt gaaagccaaa taaacaacgc agcaactcgt
    22201 gatgaagtag cgcaaaaatt agctgaagca aaagcgcttg atcaagcaat gcaagcatta
    22261 cgtaatagta ttcaagatca acaacaaaca gaatctggta gcaagtttat caatgaagat
    22321 aaaccgcaaa aagatgctta ccaagcagca gttcaaaatg caaaagattt aattaaccaa
    22381 acaggtaatc caacgcttga taaagcacaa gttgaacaat tgacacaagc agtaacaact
    22441 gcaaaagata acctacatgg tgatcaaaaa cttgctcgtg atcaacaaca agcagtaaca
    22501 actgtaaatg cattgccaaa cataaatcat gcacaacaac aagcattaac tgatgctata
    22561 aatgcagcgc ctacaagaac agaggttgca caacatgttc aaactgctac tgaacttgat
    22621 cacgcgatgg aaacattgaa aaataaagtt gatcaagtaa atacagataa ggctcaacca
    22681 aattacactg aagcgtcaac tgataaaaaa gaagcagtag accaagcgtt acaagctgca
    22741 gaaagcatta cagatccaac taatggttca aatgcgaata aagacgctgt agaacaagca
    22801 ttaactaagc ttcaagaaaa agtaaatgag ttaaatggtg atgagagagt cgctgaagct
    22861 aaaacacaag cgaaacaaaa tattgaccaa ttaacacatt taaatgctga tcaaatcgca
    22921 actgctaaac aaaacattga tcaagcgacg caacttcaac caatcgctga attagtagat
    22981 caagcaacgc aattgaacca atcaatggat caattacaac aagctgttaa tgatcataca
    23041 aatgttgagc aaacagttga ttacacgcaa gcagattcag ataagcaaaa ggcttataaa
    23101 caagcgattg ctgatgctga aaatgtattg aaacaaaatg cgaataagca acaagtggat
    23161 caagcacttc aaaatatttt aaatgcaaaa caagcattaa atggtgatga acgtgtagca
    23221 cttgctaaaa caaatggtaa acatgacatc gaccaattga atgcattaaa caatgctcaa
    23281 caagatggat ttaaaggtcg catcgatcaa tcaaacgatt taaatcaaat ccaacaaatt
    23341 gtagatgagg ctaaggcact taatcgtgca atggatcaat tgtcagaaga aatcactggc
    23401 aatgaaggac gcacgaaagg tagcacgaac tatgtcaatg cagatacaca agtcaaacaa
    23461 gtatatgatg aagcggttga taaagcgaaa caagcacttg ataaatcgac tggtcaaaac
    23521 ttaactgcag aacaagttat caaattaaat gatgcagtaa cggcagcgaa acaggcatta
    23581 aatggtgaag aaagacttaa taatcgtaag tctgaagcat tacaaagatt ggatcaatta
    23641 acacatctaa acaatgctca aagacaatta gcaatccaac aaattaataa tgctgaaacg
    23701 ctaaataaag catctcgagc aattaataga gcaactaaat tagataatgc aatgggtgca
    23761 gtacaacaat atattgacga acagcacctt ggtgttatca gcagcacaaa ttacatcaat
    23821 gcagatgaca atttgaaagc aaattatgat aatgcaattg cgaatgcagc acatgagtta
    23881 gataaagtgc aaggtaatgc aattgcaaaa gctgaagcag agcaattgaa acaaaatatt
    23941 atcgatgctc aaaatgcatt aaatggagac caaaaccttg caaatgccaa agataaagca
    24001 aatgcgtttg ttaattcgtt aaatggatta aatcaacagc aacaagatct tgcacataaa
    24061 gcaattaaca atgccgatac tgtatcagat gtaacagata ttgttaataa tcaaattgac
    24121 ttaaatgatg caatggaaac attgaaacat ttagttgaca atgaaattcc aaatgcagag
    24181 caaactgtca attaccaaaa cgctgacgat aatgctaaaa caaacttcga tgatgccaaa
    24241 cgtctagcaa atgcattgct aaatagtgat aacacaaatg tgaatgatat caatggcgca
    24301 atccaagcag tcaatgatgc aatccataat cttaatggtg atcaacgact acaagatgct
    24361 aaagacaagg caattcaatc tattaatcaa gctttagcta ataagctaaa agaaatcgaa
    24421 gcttcaaatg cgacggatca agacaagctt attgcgaaaa ataaagcaga agaattggca
    24481 aacagcatca tcaacaacat taataaagca acaagtaatc aggatgtatc tcaagttcaa
    24541 acagctggca accatgcgat tgaacaagtg catgccaatg aaataccgaa agctaaaatt
    24601 gatgcgaata aagatgttga caagcaagtt caagcattaa ttgacgaaat tgatcgaaat
    24661 ccaaatctaa cagataagga aaaacaagca cttaaagatc gtattaatca aatacttcaa
    24721 caaggtcata acgacattaa caatgcgctg actaaagaag aaattgaaca agccaaagca
    24781 caacttgcgc aagcattaca agaaattaaa gatttagtga aagctaaaga aaatgcgaaa
    24841 caagatgttg acaagcaagt tcaagcttta attgacgaaa tcgatcaaaa tccaaatcta
    24901 acagataagg aaaaacaagc acttaaagat cgaattaacc aaatacttca acaaggtcat
    24961 aacgacatta acaatgcgat gactaaagaa gaaattgaac aagccaaagc acaacttgca
    25021 caagcattac aagacatcaa agatttagtg aaagctaaag aagatgcgaa aaatgcaata
    25081 aaagccttag ctaatgcgaa gcgtgatcaa atcaattcaa atccagattt aacacctgag
    25141 caaaaagcaa aagcgctcaa agaaattgac gaagctgaaa aacgagcact acaaaacgtt
    25201 gagaatgctc aaactataga tcaattaaat cgaggattaa acttaggttt agatgacatt
    25261 agaaatacac atgtatggga ggttgatgaa caacctgctg taaatgaaat ttttgaagca
    25321 acacctgagc aaatcctagt taatggtgaa ctcattgtac atcgtgatga catcattaca
    25381 gaacaagata ttcttgcaca cataaactta attgatcagc tttcagcaga agttattgat
    25441 acaccatcaa ctgcaacgat ttctgatagc ttaacagcaa aagttgaagt tacattgctt
    25501 gatggatcaa aagtgattgt taatgttcct gtaaaagttg tagaaaaaga attgtcagta
    25561 gtcaaacaac aggcaattga atcaatcgaa aatgcggcac aacaaaagat taatgaaatc
    25621 aataatagtg tgacattaac actggaacaa aaagaagctg caattgcaga agttaataag
    25681 cttaaacaac aagcaattga tcatattaac aatgcacctg atgttcattc agttgaagaa
    25741 attcaacaac aagaacaagc gcatattgaa caatttaatc cagaacaatt tacgattgaa
    25801 caagcaaaat caaatgcaat taaatcgatt gaagatgcaa ttcaacatat gattgatgaa
    25861 atcaaagctc gtactgatct aacagataaa gagaagcaag aagctattgc taagttaaat
    25921 caattaaaag aacaagcaat tcaagcgatt caacgtgcgc aaagcatcga tgaaataact
    25981 gagcaattgg aacaatttaa agctcaaatg aaagcagcta atccaacagc aaaagaacta
    26041 gctaaacgca agcaagaagc tattagtaga attaaagact tttcaaatga aaaaatgaat
    26101 agtattcgaa atagtgaaat tggcacagct gatgaaaaac aagcagcaat gaatcaaatt
    26161 aacgaaattg tgcttgaaac aattagagat attaataatg cgcatacatt acagcaagtt
    26221 gaggctgcat tgaacaatgg tattgctcga atttcagcag tacaaattgt aatatctgat
    26281 cgtgctaaac aatcttcaag tactggaaat gaatctaata gccatttaac aattggttat
    26341 ggaactgcaa atcatccatt taacagttcg actattggac ataaaaagaa aattgatgaa
    26401 gatgatgaca ttgatccact tcatatgcgt cactttagta ataatttcgg taatgttatt
    26461 aaaaacgcta ttggtgtggt gggtatctct ggcttactag ctagtttctg gttcttcatt
    26521 gccaaacgtc gtcgtaaaga agatgaagag gaagaattag aaataagaga taataataaa
    26581 gattcaataa aagagacttt agacgataca aaacatttac cacttttatt tgcgaaacgt
    26641 cgcagaaaag aagatgaaga agatgttact gttgaagaaa aagattcgct aaataatggc
    26701 gagtcactcg ataaagttaa acatacgcct ttcttcttac caaaacgtcg tcgtaaagaa
    26761 gatgaagaag atgtggaagt tacaaatgaa aacacagatg aaaaagtgtt gaaagataac
    26821 gaacattcac cactcttatt cgcaaaacga cgcaaagata aagaggaaga tgttgaaaca
    26881 acaactagta ttgaatctaa agatgaggac gttcctttat tattggctaa aaagaaaaat
    26941 caaaaagata accaatccaa agacaaaaag tcagcatcaa aaaatacttc taaaaaggta
    27001 gcagccaaaa agaagaaaaa gaaatctaag aaaaataaaa aataatttgt ttctttgata
    27061 aatagaggag caccgattga catcacatca gtcggtgctc cttttattta ttctttttaa
    27121 ttaatttata caatgcctgt tgagcgtgtt gattcgcttc tttgttttgt tctctcggta
    27181 tccatttaac aaataataaa tcaaaatctt tttcaaatat ttctatttga tcaaaataag
    27241 gtttgaaatt tgcgtttttc acataaccag cttcaatgct atctgcaatt atctttgagt
    27301 ctgtatataa tagtgcgttt tgaacattta attcacgtgc atgttctagt gcataaatac
    27361 atgcagccca ttctgcagtg tggttatcca tttcgcctaa ctcatgtgta tatgtataat
    27421 gctgctcatc ttctttgatt acaatggcac atgtacttat gcctggattt cctttcgtcg
    27481 cagcatcaaa atttattttc gccataataa acctactttc tattcaatac ttagttaaag
    27541 ttactattac tgtaatacaa aatatgttgg gtaatccatt aaaaaacacg catcacttaa
    27601 ataagtaaca cgtgtttaaa atactcgctg attcaaagat gattttctaa tacgtagtac
    27661 tgtaatatac ttcctaaaaa atcatcttca ggctgggaca taaatcaatg ttctatgctc
    27721 tacgatgtta tattggcagt agttgactga acaaaaatgc gcttgtaaca agcttttttc
    27781 aattctagtc aggggcccca acacagagaa tttcgaaaag aaattctaca ggcaatgcga
    27841 gttggggtgt gggccccaac acagaagatg acgaaaagtc agcttacaat aatgtgcaag
    27901 ttggggatgg gccccaacaa agagaaattg gattcccaat ttctacagac aatgcaagtt
    27961 ggggtgggac gacgaaataa attttgcgaa aatatcattt ctgtc
//
LOCUS       DAMZKC010000017        27898 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.017, whole genome shotgun sequence.
ACCESSION   DAMZKC010000017 DAMZKC010000000
VERSION     DAMZKC010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 27898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27898
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.017"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            142..3215
                     /gene="mupA"
                     /locus_tag="POB55_000509"
                     /pseudo
     CDS             142..3215
                     /gene="mupA"
                     /locus_tag="POB55_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163435.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mupirocin-resistant isoleucine--tRNA ligase
                     MupA"
     gene            3285..4172
                     /locus_tag="POB55_000510"
     CDS             3285..4172
                     /locus_tag="POB55_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HDI6499500.1"
                     /translation="MYNFDYSKLPIKNIQKIFPIAGGYVNLSFSVDASNKKYFLKLQP
                     NTKSNFFDYELSSLKELTDKNIPVPQIINKGELDNNSFLLLEFIENGHAYPESYRKLG
                     KIVANMHKNINSLNLFGFSHNFNGGTIEFTNKWTSSWGDLFIKSRMDKLCSEIYKKRL
                     FSISDLLLYEDIRKIMLKSLYYHQSSPSLLHGDLWKGNILFQKNGDPILCDPVCLYGD
                     REFDIGYTLALEKFPLDFYQEYNNIYPLCVGYEKRIEFYKLYVFMIHLFAIGTDFYDK
                     VKDTMYNILNNYGVDFIKG"
     gene            4517..4810
                     /locus_tag="POB55_000511"
     CDS             4517..4810
                     /locus_tag="POB55_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000726503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HDI6499501.1"
                     /translation="MKKKFVSSCIASTILFGTLLGVTYKAEAATVHVAGGVWSHGIGK
                     HYVWSYYSHNKRNHGSTAVGKYSSFSGVARPGVQSKASAPKAWGGNKTFYSLH"
     gene            4885..6819
                     /locus_tag="POB55_000512"
     CDS             4885..6819
                     /locus_tag="POB55_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001670421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HDI6499502.1"
                     /translation="MKKFKLFLDFSTLLLISGLLFLFFFKENEEIIPESSNILTISNW
                     DKSNSKSKVLDVIESGAKNQNIQIIKSVKDFDNKKEFFVFNSKRNNSDFIRNKTSLLT
                     PSDLLNREIKGKYYIIGEHFNVEELTSELKKAGLTSEVEYINRNILFFELVIDNDLLV
                     PFIFLGVLYLLYFLYDRGYNLKFYAIQKLHGFSTTRIIFHNIHIKIIYWLVLTAIFFI
                     ANILIIHIANLELDFLMFIRRFIVLLFVFACITTLLWLISYSLLLKLSIPLILKGKKG
                     YKFSIFMGTFTKCIMVLILSFLLAQNLNAYTKLKNIYDSEKIWQKLDNYYTLEISPNI
                     RNSEEKQILETNIYNLIKKSEQLGGLLLKNNNIANPDKNNYIPENGNVFFINNNFLNF
                     YKNINHDFKMIDNHSDKINVFIPPRLQYQKSEIQKNHQGWIDFQKQQNKKVDVQVLSK
                     NVSVFSFDRTTNLKFGYLDSPIVMLLTLDDLTGDFYLAAVSQGGYLFKDLDTLKQLLK
                     DNHLEEEISGITNYKDSVLNELNETKTKMIITIVTIIINVFILLIATVFETIQYFSWN
                     KKQLLLRKIHGYSLFSSNVRYLTISILLSIMLAYTTHILFGSKILLFIIMSIAIIQHL
                     LQIAYIKYLEKYFKDLMREI"
     gene            6823..7134
                     /locus_tag="POB55_000513"
     CDS             6823..7134
                     /locus_tag="POB55_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDI6499503.1"
                     /translation="MKRTLIIVTIVLLIIILSLLLVRNEHLDRFNPLLKEKVSYAKVP
                     KDTQDYRNITVYSKNGEKKSYKLDFLGYDPTKEYVKVNHKGKYVRSIEYITKDIPSFL
                     K"
     gene            7146..7772
                     /locus_tag="POB55_000514"
     CDS             7146..7772
                     /locus_tag="POB55_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6499504.1"
                     /translation="MIKLENVFVKKGNKNILDGCNFNFEKGKSYALVGESGAGKSTLL
                     NIIAGFEDVSQGSIYIEDKLLKKKVDFYRYTLGYLFQNFALLENQTISQNLDLALKFK
                     KNKKDNMNLKKEVLKKVGLDLDIKRIVSSLSGGEQQRVALARLILKDPKIILADEPTG
                     SLDTKNGKIVIDLLLKLLDENKTMIVVTHDLELAKRFDVIVNISELRS"
     gene            8010..8400
                     /locus_tag="POB55_000515"
                     /pseudo
     CDS             8010..8400
                     /locus_tag="POB55_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001643491.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
     gene            8340..8639
                     /locus_tag="POB55_000516"
     CDS             8340..8639
                     /locus_tag="POB55_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HDI6499505.1"
                     /translation="MEEEYGKENLLYATVHMDEITPHMHYGVVPITKDGRLSAKEVVG
                     NKKALTEFQDRFNTYINKQGYDLKRGISRQLTKEKHDQVSRYKQKTEYHKQMHMR"
     gene            8733..9278
                     /locus_tag="POB55_000517"
     CDS             8733..9278
                     /locus_tag="POB55_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001805533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HDI6499506.1"
                     /translation="MYEIKTKNVGGWFHKEKQETGNIVITKTDFEKYTKQIKAAQMIL
                     DDYECIKSGKSLKKAEKQNESLVNELTSVHMENEKLVEEFNDLAQRYNYLLSENEKKD
                     KELNYTLKLFNQVFKIIKSMMKEERYHTLINHIDNHLDNSKIREVMTIDNNDEQFFKK
                     KYQAQEREIIFKEDREDGYTL"
     gene            9662..10522
                     /locus_tag="POB55_000518"
     CDS             9662..10522
                     /locus_tag="POB55_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDI6499507.1"
                     /translation="MTGETVVYKNEMNLVPLRRFTATEINLFFAMCNKLKEQDTNTLR
                     LSFDELKKLSNYSPETRNINRFANDLDNVYKKMLNLTIRYEDDDVIERFVLFNHYRIH
                     KREQYLEISTSSNLKHILNSITNNFTKFELKEMTRLKSTYSKNMFRLLKQYKHTGYLK
                     IHIDDFKNRLDIPKSYRMTDINKNVFKPIIIELGSIFNNLTINKIKAKKGRKIEWIEF
                     TFDAEKRIHNKRQPQMSKIDKSRQYVRREKTPKWLEERSYEKQPQKDYDPQLEKERED
                     FLKQLELNWE"
     gene            10609..10950
                     /locus_tag="POB55_000519"
     CDS             10609..10950
                     /locus_tag="POB55_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI6499508.1"
                     /translation="MKLAEAIKEQRELKGWSQEELANILKVSRQSVSKWESAKNYPSL
                     DILIAMSDLFGISLEHLIKGDARFKKVILEGNYRESNRVPSIVDFLYDFWWLFFPVAG
                     FLVWAIHSFMG"
     gene            complement(11189..11260)
                     /locus_tag="POB55_000520"
     CDS             complement(11189..11260)
                     /locus_tag="POB55_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HDI6499509.1"
                     /translation="MNEILVHIMTTAISGCLVTLFGY"
     gene            11409..11594
                     /locus_tag="POB55_000521"
     CDS             11409..11594
                     /locus_tag="POB55_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499510.1"
                     /translation="MFGDQRQEATKYVIKEGYQDIYFLNKNGEWYYFEVRSVWRGKHI
                     IRVKDGLLGWRKEIVTE"
     gene            complement(11813..12556)
                     /locus_tag="POB55_000522"
     CDS             complement(11813..12556)
                     /locus_tag="POB55_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HDI6499511.1"
                     /translation="MKSVKRLSEELGVSKQTIFNNIKRLNIETIKQENTSFIKEDTDI
                     EKIIQRVNENKKKYGFESATEDKQKKESDNINFESKSDAQIVEILKNQINTLNNQIEK
                     QESRHETTIEFYRKELQERSKLLENQQVLALESNKKIQKLENQLEKEKHLNYPFDTST
                     NARQNVDAQEKTYTTSPVNINRDQEETKETEIQYKDISGSQSDESTQGEEAQREDVSA
                     NPNDNDSDIEEKSEETEAKKGFWSRLFGN"
     gene            12967..13643
                     /locus_tag="POB55_000523"
                     /pseudo
     CDS             12967..13643
                     /locus_tag="POB55_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181101.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
     gene            15145..15762
                     /gene="cadD"
                     /locus_tag="POB55_000524"
     CDS             15145..15762
                     /gene="cadD"
                     /locus_tag="POB55_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium resistance transporter CadD"
                     /protein_id="HDI6499512.1"
                     /translation="MIQTVVAAAVLYIATAVDLLVILLIFFARAKTRKEYRNIYVGQY
                     LGSIILILVSLFLAFVLNYVPEKWILGLLGLIPIYLGIKVAIYDDCEGEKRAKKELNE
                     KGLSKLVGTVAIVTIASCGADNIGLFVPYFVTLSVTNLLLTLFVFLILIFFLVFTAQK
                     LANIPGIGEIVEKFSRWIMAIIYIALGLFIIIENDTIQTILGFIF"
     gene            15781..16128
                     /locus_tag="POB55_000525"
     CDS             15781..16128
                     /locus_tag="POB55_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020366422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI6499513.1"
                     /translation="MSYENTCDVICVHEDKVNNALSFLEDDKSKKLLNILEKICDEKK
                     LKIILSLIKEDELCVCDISLILKMSVASTSHHLRLLYKNEVLDFYKDGKMAYYFIKDD
                     EIREFFSKNQEGF"
     gene            16297..16389
                     /locus_tag="POB55_000526"
     CDS             16297..16389
                     /locus_tag="POB55_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HDI6499514.1"
                     /translation="MLIIFVHIIAPVISGCAVAYYTYWLSKRNK"
     gene            16601..17125
                     /locus_tag="POB55_000527"
     CDS             16601..17125
                     /locus_tag="POB55_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HDI6499515.1"
                     /translation="MSNTKIKYQIFISSTYLDLIEERQAVVEAVLNSNNIPAGMELFN
                     SSSKSQWEIITQWINESDAVILLLGSSYGTIDSEENISYTEMEYNFAKSLGKPIYVFE
                     MNKASTESAEKSKKLDEFKTKVKENRLISPNINDISELKSKVSQTLIQSVPSEIKENS
                     GWIRVQDCGKNVKK"
     gene            17197..17574
                     /locus_tag="POB55_000528"
     CDS             17197..17574
                     /locus_tag="POB55_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499516.1"
                     /translation="MYVKTFYFQHTPFDDSYIEDIENYLLNYENNPHKSFMKIKVYDY
                     KNFLKHLKNILILIKQGKKPLAQAGHFKLSFYEEDLKLLQKNYKATLVDIKGLNKHFY
                     KECDRCLKAYNIFIEGINKHLYE"
     gene            complement(17694..18509)
                     /locus_tag="POB55_000529"
     CDS             complement(17694..18509)
                     /locus_tag="POB55_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDI6499517.1"
                     /translation="MTKNQNFIETKEYKRFAEFCDACIKYQYIGICYGQPGVGKTLSS
                     RYYTNWDTIEKQVNHRGWEDLASKTTDDILSVDKIFYNAPAEKQTKLRNDLYSITAII
                     DLGQKLHVVNKYGHEHSKHYSDMFKYIDLIIVDEIDRLKVQHLEQLRAIYDEHNLAMI
                     FIGMPGIEKKLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKCQDLGFDLKLEDFTDY
                     EAITTIIKITKGNFRLIHRLFAQIDRIMDINGLDKISTEVVETARDSLVIGIR"
     gene            complement(18502..19945)
                     /locus_tag="POB55_000530"
                     /pseudo
     CDS             complement(18502..19945)
                     /locus_tag="POB55_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790716.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            complement(19917..20510)
                     /locus_tag="POB55_000531"
     CDS             complement(19917..20510)
                     /locus_tag="POB55_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDI6499518.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNTYGCEKIFNDHMSGSKSKRPG
                     LDKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSST
                     GQLLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNQKDLNLLKTLYDNG
                     TPIKTIAEQWHVSRTTIYRYLNKLEDKEDEKQGEVSN"
     gene            complement(20774..21154)
                     /gene="blaI"
                     /locus_tag="POB55_000532"
     CDS             complement(20774..21154)
                     /gene="blaI"
                     /locus_tag="POB55_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillinase repressor BlaI"
                     /protein_id="HDI6499519.1"
                     /translation="MANKQVEISMAEWDVMNIIWDKKSVSANEIVVEIQKYKEVSDKT
                     IRTLITRLYKKEIIKRYKSENIYFYSSNIKEDDIKMKTAKTFLNKLYGGDMKSLVLNF
                     AKNEELNNKEIEELRDILNDISKK"
     gene            complement(21144..22901)
                     /gene="blaR1"
                     /locus_tag="POB55_000533"
     CDS             complement(21144..22901)
                     /gene="blaR1"
                     /locus_tag="POB55_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer BlaR1"
                     /protein_id="HDI6499520.1"
                     /translation="MAKLLIMSVVSFCFIFLLLVFFRYILKRYFNYSLNYKVWYLTVL
                     AGLIPFIPIKFSFIKFNNVNNQAPTVESKSHDLNHNIITTKPVQEFATDIHKFNWDSI
                     DNICTVIWIVLVIILSFKFLKALLYLKYLKKQSLYLNENEKNKIDTILFNHQYKKNIV
                     IRKAEAIQSPITFWYGKYIILIPSSYFKSVIDKRLKYIILHEYAHAKNRDTLHLIIFN
                     IFSIIMSYNPLVHIVKRKIIHDNEVEADRFVLNNINKNEFKTYAESIMDSVLKTSFFN
                     KNILSHSFNGKKSLLKNRLINIKEADLKKQSKLILIFICIFTFFIMIIQSQFLMGQSL
                     TDYNYKKPLQSDYQILDESKNFGSNSGSFVMYSMKKDKYYIYNEKESRKRYSPDSTYK
                     IYLALFGLDRHIISDKNSRMSWNHKHYLFESWNKEQDLNTAMQNSVNWYFERISNQIP
                     KNYTAAQLKQLNYGNENLGSYKSYWMEDSLKISNLEQVIVFKNMMEQNNHFSKKAKNQ
                     LSSSLLIKKNEKYELYGKTGTGIVNGKYNNGWFVGYVITNHDKYYFATHLSDGNPSGK
                     NAELISEKILKGMGVLNGQ"
     gene            23008..23853
                     /gene="blaZ"
                     /locus_tag="POB55_000534"
     CDS             23008..23853
                     /gene="blaZ"
                     /locus_tag="POB55_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /protein_id="HDI6499521.1"
                     /translation="MKKLIFLIVMALVLSACNSNSSHAKELNDLEKKYNAHIGVYALD
                     TKSGKEVKFNSDKRFAYASTSKAINSAILLEQVPYNKLNKKVHINKDDIVAYSPILEK
                     YVGKDITLKELIEASMTYSDNTANNKIIKEIGGIKKVKQRLKELGDKVTNPVRYEIEL
                     NYYSPKSKKDTSTPAAFGKTLNKLIANGKLSKKNKNFLLDLMFNNKNGDTLIKDGVPK
                     DYKVADKSGQAITYASRNDVAFVYPKGQSEPIVLVIFTNKDNKSDKPNDKLISETAKS
                     VMKEF"
     gene            24222..24755
                     /locus_tag="POB55_000535"
     CDS             24222..24755
                     /locus_tag="POB55_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDI6499522.1"
                     /translation="MIVGYARVSSNDQNLERQLENLKTFCAEKIFTEKQSGKSIENRP
                     VLQEALNFLRMGDRFVVESIDCLGRNYDEVVGNPLLDKFMKDLIIQILAMVSEQERNE
                     SKRRQAQGIQVAKEKGVYKGRPLLYSPNAKDPQKRIIYHRVVEMLEEGQSISKIAKEV
                     NITRQTIYRIKNDNDLI"
     gene            complement(24937..25503)
                     /gene="qacR"
                     /locus_tag="POB55_000536"
     CDS             complement(24937..25503)
                     /gene="qacR"
                     /locus_tag="POB55_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001807342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug-binding transcriptional regulator
                     QacR"
                     /protein_id="HDI6499523.1"
                     /translation="MNLKDKILGVAKELFIKNGYNATTTGEIVKLSESSKGNLYYHFK
                     TKENLFLEILNIEESKWQEQWKKEQIKCKTNREKFYLYNELSLTTEYYYPLQNAIIEF
                     YTEYYKTNSINEKMNKLENKYIDAYHVIFKEGNLNGEWCINDVNAVSKIAANAVNGIV
                     TFTHEQNINERIKLMNKFSQIFLNGLSK"
     gene            25682..27226
                     /locus_tag="POB55_000537"
     CDS             25682..27226
                     /locus_tag="POB55_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux MFS
                     transporter QacA"
                     /protein_id="HDI6499524.1"
                     /translation="MISFFTKTTDMMTSKKRWTALVVLAVSLFVVTMDMTILIMALPE
                     LVRELEPSGTQQLWIVDIYSLVLAGFIIPLSAFADKWGRKKALLTGFALFGLVSLAIF
                     FAESAEFVIAIRFLLGIAGALIMPTTLSMIRVIFENPKERATALAVWSIASSIGAVFG
                     PIIGGALLEQFSWHSAFLINVPFAIIAVVAGLFLLPESKLSKEKSHSWDIPSTILSIA
                     GMIGLVWSIKEFSKEGLADIIPWVVIVLAITMIVIFVKRNLSSSDPMLDVRLFKKRSF
                     SAGTIAAFMTMFAMASVLLLASQWLQVVEELSPFKAGLYLLPMAIGDMVFAPIAPGLA
                     ARFGPKIVLPSGIGIAAIGMFIMYFFGHPLSYSTMALALILVGAGMASLAVASALIML
                     ETPTSKAGNAAAVEESMYDLGNVFGVAVLGSLSSMLYRVFLDISSFSSKGIVGDLAHV
                     AEESVVGAVEVAKATGIKQLANEAVTSFNDAFVATALVGGIIMIIISIVVYLLIPKSL
                     DITKQK"
     gene            27431..27679
                     /locus_tag="POB55_000538"
                     /pseudo
     CDS             27431..27679
                     /locus_tag="POB55_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077893.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        10136 a         3674 c         4271 g         9817 t
ORIGIN      
        1 ttgcagtagt ttgtattata actttacttt atccaatata tctttctatg gagacatgaa
       61 aggaaatgaa tttcatgaaa attcaccaag actaatagta aaaataacga aataagtgat
      121 actctaggag gctgaaaagt tttgacaaag aaatatttaa acacccagaa tgaaatatca
      181 gcattttgga atactcaaaa gatatttaaa aaatcaattg acaatagaaa aggacaggaa
      241 agttttgttt tttatgacgg ccccccaact gcaaatggcc ttcctcatgc tggccatgtt
      301 cttggaagag taatcaagga tttagttgca agattaaaaa ctatgcaagg tttttatgta
      361 gaaagaaaag caggatggga tacccatggc ttaccagttg aattagaggt tgaaaaaaaa
      421 ttggaattaa aggaaaacaa gacattgaaa agtatggaat agaaaatttt ataaatgaat
      481 gtaaaaaaag tgtatttaat tatgaaaaag aatggcggga tttttctaaa gatttaggat
      541 actgggttga catggactcc ccctatataa ctcttgagaa taattatatt gaaagtgtat
      601 ggaatatatt atctacattc cataaaaaag gactattata taagggacat aaggtgactc
      661 cttattgtac acatgatcaa accgctttaa gttctcatga agtagcgcaa ggctataaaa
      721 acgttaaaga tttatcagct gttgttaaat ttcaacttac aaatagtaaa gatacttatt
      781 tcttaagttg gactaccact ccctggactt tgcctgcaaa tgtagcatta gctataaata
      841 aagatcttaa ttattcaaaa attcgggtag aaaatgagta ttatatctta gctacagatc
      901 taattaattc tataataact gaaaaatacg aaattattga taccttttca ggaagtaatt
      961 taattaattt aaaatacatt cctccttttg aaagcgacgg tttagttaat gcatattacg
     1021 ttgttgatgg agaatttgtt actaactcag aaggaactgg tattgttcat atagcaccag
     1081 ctcatgggga agatgactac caattggttt tagagcgtga tttggatttc ttaaatgtta
     1141 taacaagaga aggagtatat aatgataggt tccctgaatt agttggtaat aaagctaaaa
     1201 atagtgatat agaaatcata aaattattat ccaaaaaaca acttttatat aaaaaacaaa
     1261 aatatgagca taattatcct cattgttgga gatgtggtaa tcctttgata tattatgcga
     1321 tggaaggttg gtttattaaa acaactaatt ttaagaatga aattattaac aataataata
     1381 atatagagtg gtttccttct catattaagg aagggagaat gggaaatttc ttagaaaata
     1441 tggttgattg gaacattggt agaaatagat attggggaac accattaaat gtatggattt
     1501 gcaatgattg taatcacgaa tacgcaccaa gtagtattaa ggatttacaa aataattcca
     1561 tcaataaaat tgatgaagat attgagttgc atagacctta tgttgataat atcactctta
     1621 gttgccctaa gtgtaatggg aaaatgtctc gagtagaaga agtaatcgat gtttggtttg
     1681 atagcggctc tatgccgttt gctcagcatc attatccttt tgataaccag aaaattttta
     1741 atcaacactt tccagctgat tttattgcag aaggagttga tcaaacgaga ggctggtttt
     1801 acagtttact agtaatttct actattctaa aaggaaaatc ttcttataaa cgtgctttat
     1861 ctttaggaca tattctagac agtaatggta aaaaaatgtc taaaagtaaa ggaaacgtta
     1921 ttaatccaac tgaattaatt aataagtacg gagccgattc tttaagatgg gccttaattt
     1981 cggatagtgc tccatggaat aacaaaagat tctcagaaaa tatagtagct cagaccaaat
     2041 cgaaatttat agatacgctt gataatattt ataaatttta taatatgtat aataaaatag
     2101 atcactataa tcctaataat gaaattacaa aaagtagaaa tacattagat aattgggctc
     2161 tttctcgctt aaacacctta ataaaagaaa gtaatattta tgtaaataat tacgatttca
     2221 cttccgcagc cagattaatt aacgaatata ccaatacaat aagtaattgg tatattcgga
     2281 gatcgagagg acgattttgg gaacaaggaa tttctaacga taaaaaagat gcgtacaata
     2341 cgctttatga aattttaaca actttatcaa gactagtggc tccatttgtt ccatttatat
     2401 ctgaaaaaat ccattataat ttgactggaa aaagtgtgca tttacaagat tatccacaat
     2461 ataaagaaag ttttattaat caagcattgg aagatgaaat gcataccgtt ataaaaattg
     2521 tagaattatc tagacaggct cgcaaaaatg cagatttaaa aattaagcaa cctttatcga
     2581 aaatggtgat taaacctaat agtcaattaa acttaagttt tttacctaat tactattcaa
     2641 taataaaaga cgaattaaat ataaaaaaca ttgaattaac tgataatatt aatgactata
     2701 ttacctatga gcttaaattg aatttttctt ctgtgggacc aaaactaggg aacaaaacga
     2761 aaaatattca aacattgata gactccctat cagagtatga taaaaaaagt ttaattgagt
     2821 ctaataactt caaaagttta tcttctgatg ctgagttaac taaggatgat tttataatta
     2881 aaaccttacc taaggatagt tatcaactca gtgaagataa tgactgcgtt atattattag
     2941 ataaaaattt atctcctgaa ttaattcgcg aaggacatgc tagagagctc attagattaa
     3001 ttcaacaatt aagaaaaaag aaaaatttac caataaatca acgtattgat atttatatcg
     3061 gtgtaactgg ggaattatta gaatcaataa aaaccaataa aaatatgttt aaagaaaatt
     3121 tgctgattaa aaatatacac ttaaatgtta tagatgaata tgaaaatact attcatttta
     3181 ataataaaga aataaaaatt tccttattat attaaacaaa catggccact ctattttagt
     3241 agagtggcca tgagttatca aagccaagca aaaggtgata aactatgtat aattttgatt
     3301 attctaaatt accaattaaa aatattcaaa aaatatttcc tattgcaggg ggatatgtga
     3361 atctatcttt ttcagttgat gcttccaaca aaaaatattt tttaaagcta caacctaata
     3421 ctaaatcaaa tttttttgat tatgagttat ccagtttaaa agaattaact gataaaaata
     3481 ttcctgttcc tcaaattatt aataaaggag aattagacaa taattctttt ttactgttag
     3541 agttcattga aaatggccat gcttatcctg aaagctatag aaaactaggt aaaatagttg
     3601 caaacatgca taaaaatata aattcactaa atctatttgg ctttagtcat aatttcaatg
     3661 gaggaacgat tgaattcact aataaatgga cttcttcttg gggggattta tttattaaaa
     3721 gtaggatgga taagctatgt tctgaaatat acaagaaacg tttattttca atttcggatc
     3781 ttttattata tgaagatata cgaaaaatta tgttgaagag cttatattat caccaatcgt
     3841 caccatcatt acttcatggt gatctttgga aaggaaatat tctttttcaa aaaaatggag
     3901 atcctattct atgtgatcca gtctgtttat atggagatag agaatttgat attggatata
     3961 ctctagcatt agaaaaattt ccattagatt tttatcaaga atataataat atatatccgt
     4021 tgtgtgttgg atatgaaaag cgaatagaat tttacaagtt atatgtgttt atgattcatt
     4081 tatttgctat aggtacagat ttttatgata aagttaaaga taccatgtat aatattttaa
     4141 ataattacgg ggttgatttt ataaaaggat agggtgttga ctttgtttca attttctaaa
     4201 atcatttatc tacttctaat agtcactttt ttcgaacgtt tataacgcaa tcatgatgag
     4261 agtattaata cagttaatta tttgaaatac tttagtttat ataatgttga attataggta
     4321 gtgaaattgt atttattata cattgtcata tcgttgttta atgatagtta atttaaggat
     4381 aatagaatta atattatata tagatgaaca aaacaagcaa cagagcagaa aaaacacttt
     4441 tagattattt ttactcattc tctattttat ttatttttgc tataataaat aaataatatt
     4501 aaatggaggt gtttttatga aaaagaaatt tgtttctagt tgtattgcta gtacaatctt
     4561 attcggcact ttattaggtg taacttataa agctgaagct gcaacagtac atgtagctgg
     4621 tggtgtttgg agccatggta ttggaaaaca ttacgtatgg tcttattata gtcacaataa
     4681 aagaaatcat ggttcaacag cagtagggaa atattcatct tttagtggtg ttgctcgacc
     4741 tggtgttcaa tctaaggcat ctgctccaaa ggcttggggt ggaaacaaaa cattttatag
     4801 tcttcactaa ttacatatat taattaagga gaatagacag aattaagtaa acttaattct
     4861 gtctattctc ttgaaagagg caatatgaaa aaattcaaat tatttttaga ttttagtact
     4921 ttattattaa tttcagggct attattttta tttttcttca aagaaaatga agaaattata
     4981 cctgaaagtt ccaatatttt aacaatctct aattgggata aatctaacag taaatcaaaa
     5041 gtgttagatg tcattgaatc aggcgccaaa aatcaaaata tacaaataat taaatccgtt
     5101 aaagattttg ataataaaaa agaatttttt gttttcaact caaaacgaaa taattctgac
     5161 tttatacgta ataaaacatc acttttaact ccatcagatt tattaaacag agagataaaa
     5221 ggaaagtatt atattattgg tgaacatttc aatgtagaag agcttacgag tgaattaaaa
     5281 aaagctggtt taacatctga agttgaatac ataaatagaa acatattatt ttttgaatta
     5341 gtcatagata atgatttatt agtgcctttc atattcttag gcgtattata tcttttgtac
     5401 ttcttatatg acagaggata taatttgaaa ttttatgcta ttcaaaagct acatggcttt
     5461 tcaactacac gcattatttt tcataatatt catataaaaa tcatttattg gctagtactt
     5521 accgctattt tctttattgc taatatatta atcattcata tagccaattt agaattggat
     5581 ttcttaatgt ttattcgtag atttatcgtg ttgctttttg tttttgcttg tattactaca
     5641 ttgctgtggc tgatttcgta tagtcttctt ttgaagttaa gtattccatt aatcttaaaa
     5701 gggaaaaaag gatataagtt ttcaatattt atgggtacct tcaccaaatg tatcatggtg
     5761 ctaatactat cttttttact ggcacaaaat ttaaatgctt atacgaagtt aaagaatata
     5821 tatgactctg aaaaaatctg gcaaaagtta gataattact atacattaga gatttctcct
     5881 aatattagaa attcagaaga aaaacaaatt ttagaaacta atatatacaa tttaattaag
     5941 aaaagcgaac aacttggtgg attgctttta aagaataata atatagccaa tcccgataaa
     6001 aacaattaca ttcctgaaaa tggcaatgtc ttttttatca ataacaattt tctgaatttc
     6061 tataaaaata taaatcatga ttttaaaatg attgacaatc attcggataa aataaacgtg
     6121 tttattcctc caaggttaca atatcaaaaa tctgaaattc aaaaaaatca ccaaggatgg
     6181 attgactttc aaaagcaaca aaacaaaaaa gtagatgtgc aagtactgtc aaaaaacgta
     6241 tctgtttttt cttttgatcg cacaactaat ttgaaatttg ggtatctaga ttctccaata
     6301 gtaatgttgt tgacacttga cgatttaaca ggagattttt atttagctgc tgtttctcaa
     6361 ggtggatatc tatttaaaga tttagataca cttaaacagt tgcttaaaga taatcaccta
     6421 gaagaagaaa ttagcggtat aactaattac aaggattctg tgttaaatga attaaatgaa
     6481 acgaaaacaa aaatgataat aacaatagtt actattatta ttaacgtttt cattcttttg
     6541 atagctactg tatttgaaac aattcaatat tttagctgga ataaaaagca actattatta
     6601 agaaaaattc atggttattc attattcagc agtaatgtac gttatttaac aatttctatc
     6661 ttactttcta ttatgttagc ttacactacg catattttat ttggttcaaa gatattacta
     6721 ttcataataa tgtctattgc tattattcaa catttacttc aaattgctta tattaaatat
     6781 cttgaaaaat actttaaaga tttaatgagg gagatttaaa caatgaaaag aactttaatt
     6841 attgtaacaa ttgtactatt aatcatcata cttagtctgt tattggttag aaatgagcat
     6901 ttagatcgtt ttaacccact gttaaaagaa aaagtatctt atgccaaagt tccaaaagat
     6961 acccaggact atagaaatat aactgtatac tctaaaaatg gagaaaaaaa gagttataaa
     7021 ttggattttt taggttatga tcctacaaaa gaatatgtga aagtaaatca taaagggaaa
     7081 tatgtgcgtt cgatagaata cattacaaaa gatattccta gctttttaaa atgaggtgac
     7141 cgatagtgat aaaacttgaa aatgtttttg taaaaaaagg taataaaaac attttagacg
     7201 gatgtaactt taattttgaa aaaggaaaat catatgcttt agttggagaa agtggcgctg
     7261 gaaaatctac tttacttaac ataatcgctg ggttcgaaga tgtatcacaa ggaagcatat
     7321 atattgaaga taagttacta aaaaagaaag tcgattttta tagatatacg ttagggtatt
     7381 tgtttcaaaa ttttgcttta cttgaaaatc aaacaatcag tcaaaattta gatttagccc
     7441 taaagttcaa aaagaataaa aaagataata tgaacttaaa gaaagaagta ttaaaaaagg
     7501 tcggcctaga tttagatatc aagagaattg ttagttctct aagcggtggt gaacaacaaa
     7561 gagtagcatt ggcaagatta atattaaaag atccgaaaat cattttagct gatgaaccta
     7621 ctgggtcact agatacgaaa aatggaaaaa tagttattga tttattattg aagctacttg
     7681 atgaaaataa aactatgatt gtcgtaacgc atgatttaga gcttgcgaaa cgatttgatg
     7741 tcatagtcaa tatttctgag cttagaagtt aagaaatatt gataggtata gccatacata
     7801 gagaaaaaac aatatgaaat atgattagtc attacttttg agttttttaa tttaaggact
     7861 ggattttcca gtcctttttt atgatgataa aaaacaagcc tcgcagagca cacgttttta
     7921 atgacttatt tggtttttaa taagtctagt atgttaaaac ttttctgtgt ttaggtgtta
     7981 taatcatatg cagtggaagt tgatttatta tgtcttattc aattattcga gttgttaaag
     8041 tgaaatcaaa aacaaataca agaggtattc aaagacatat tcaaagagaa aataaaaatt
     8101 atgaaaatat agatattgat ttaagtaagt catatttaaa ctatgattta gtcaatgata
     8161 ctaagtttga ttttaataaa aaaattgatg agaaaatcga aaaaaattat aaagggaaac
     8221 gcaaaattag aacagatgcc attaaacata ttgatggatt aattacatca gataatgtgt
     8281 tttttaatca attatctgaa gaagagacaa aatagttttt taagcatgct aaagcgttct
     8341 tggaagaaga atacggtaaa gaaaatcttt tatatgcgac agtacatatg gatgagataa
     8401 caccacatat gcattatggc gtagttccta ttactaaaga tggacgttta agtgcgaaag
     8461 aagttgtagg taataaaaaa gcattaaccg aattccaaga tagattcaat acgtatatta
     8521 ataagcaagg ctatgattta aaacgaggaa tttctagaca acttacaaaa gaaaaacatg
     8581 atcaagttag ccgttataaa caaaaaacag aatatcataa acaaatgcat atgcgctaga
     8641 agcaaataga ggattattta aagcaagaaa atgataaatt gatacaaaac taccaaaaat
     8701 cattagatac gcttaaaaag cttctgagtg tcatgtatga aattaaaact aaaaatgtag
     8761 gtggatggtt tcataaagag aagcaagaaa caggcaatat tgtaattact aaaactgatt
     8821 ttgaaaaata cactaaacaa attaaagcag cacagatgat attagatgat tatgaatgta
     8881 ttaaaagtgg gaagtcatta aaaaaagcag agaaacaaaa tgaaagttta gttaatgaat
     8941 tgactagtgt ccatatggaa aatgaaaagc tggttgaaga atttaatgat ttagcacaac
     9001 gttataatta tttgttgtca gagaatgaaa agaaagataa agaactaaat tatacgctta
     9061 agttattcaa tcaagttttt aaaatcatta aaagtatgat gaaagaagag cgttatcata
     9121 cactaataaa tcatattgat aatcatttag acaattctaa aatacgtgaa gtaatgacga
     9181 tagataacaa tgatgaacag ttctttaaga agaaatatca agcacaagaa cgagaaatca
     9241 tttttaaaga agatagagaa gatggttata cattataaaa atagatattt aaatgcattt
     9301 gtttgagatt aattattttt taggatgaaa aataggtagc ccccctcgaa ctttttaata
     9361 tatttttata aatcttttta aaaacctttt taggggcttg ttcagcccta gagcaacaag
     9421 ggatttcaat gcctaaagga gtgtttttgc cacataaaag gagtgttttt gccacataaa
     9481 aggagtgttt ttgccacata aaaggagtgt ttttgccaca taaaaggagt gttttaaaaa
     9541 gttgtactcc ttttatggtt tgacattgat aataaagtag tttattgtga ataaaagttt
     9601 tcaaatggga gtgtatattc aaacgatact cctttaggtt cgtaataaag ggggctgtat
     9661 catgacagga gagacagttg tatataaaaa tgaaatgaat ttagttccat taaggcgatt
     9721 tacagctact gaaattaatt tattttttgc gatgtgtaat aaattaaaag agcaagatac
     9781 taatacatta cgtttgtctt ttgatgaatt aaaaaaatta agtaattata gtccagaaac
     9841 acgtaatatt aatagatttg ctaatgattt agataatgtg tataaaaaga tgttgaattt
     9901 aaccattaga tatgaagatg atgatgtaat agaaagattc gttttattca atcattatag
     9961 aatccataag cgagaacaat atctagaaat atcaacttct tcaaatttaa aacacatatt
    10021 gaattcaatt actaataatt ttacaaaatt tgaattaaaa gaaatgacta ggcttaaatc
    10081 tacatattct aaaaatatgt ttagattact taaacaatac aaacatacag gctatttaaa
    10141 aattcacatc gatgatttta aaaatcgttt agatatacct aaatcttatc gtatgactga
    10201 tattaacaaa aatgttttta aacctattat tattgaatta ggttctatat ttaataatct
    10261 tactatcaat aaaatcaaag cgaaaaaagg gcgtaaaatt gaatggatag agttcacatt
    10321 tgacgctgag aaacgcattc ataacaaacg acaacctcaa atgtccaaga tagataaatc
    10381 aagacaatat gtgaggcgtg aaaagacgcc taaatggtta gaagaacggt catatgaaaa
    10441 gcaacctcaa aaagattacg acccacaatt agagaaagaa cgagaggatt ttttaaaaca
    10501 acttgaactg aattgggaat aacgcaactt ttcgttgcga gttggttaac taaaagtatc
    10561 gtgacttttc aattatttta attatagttt gagttaagga gtgattttat gaaattagct
    10621 gaagctataa aagaacagag agaattaaaa ggatggtctc aagaggaatt agccaatata
    10681 ttaaaggttt ccagacaaag tgtttctaaa tgggaaagtg ccaaaaacta tccttcctta
    10741 gatattttga ttgctatgag cgatttattc ggaatctctt tagagcattt aattaaagga
    10801 gatgcacgtt ttaaaaaagt tattttagag ggaaattata gagagagtaa cagggtacct
    10861 tccatagttg attttcttta tgatttttgg tggcttttct ttcctgttgc cggattttta
    10921 gtttgggcca ttcattcgtt catgggttga ttttaaaatc aaaatttaat gcacatcagg
    10981 caatatgaaa aaggaaagta tttttacgac accttgaaat gtattgataa gaatagaaga
    11041 gtttacaaaa ggcaatatga aatatatact aaaagtaaca agaatttcgt ttgtaaacgc
    11101 accaaacccc gaactattcg ccgtagttcg gggttttttg gtgaattggc tttaatcgtc
    11161 tatattattt tttatcacgt ttatggagct aataaccaaa taacgtaacg agacaaccac
    11221 tgattgccgt tgtcatgatg tgaacaagaa tttcattcat aaatcgcacc tcctctctac
    11281 gccgaattga catctgagag atagacgacc ttcacattat aacaaatctc tccttccatg
    11341 aaataacatg aatattaata aatgttgtta tatattaaag ataaataaat ataagggtgg
    11401 gattgataat gtttggcgat caaagacaag aagctacaaa atatgtcatt aaagaaggtt
    11461 atcaagatat ttatttcctc aacaaaaacg gggaatggta ttatttcgaa gtaagaagtg
    11521 tctggcgtgg caaacatatt attagagtta aagatggttt gttaggttgg agaaaagaaa
    11581 tagtaactga ataaagatac aaaatacttg ccaatgcaaa agaatgaaaa cgcgtcaaat
    11641 caacgatgta taaaagatgc ataaaagata atgaaagtaa aaagccataa atgccgtcat
    11701 ataggcattt atggcttttt gtttggttaa tataatcgat gattatcaga agttcaattc
    11761 acataaaaaa gcacggctaa tttatgaact tgtcgtacct ttttaattaa aattagttac
    11821 caaatagacg actccaaaac ccctttttag cttccgtttc ttcactcttt tcttctatat
    11881 cactatcatt gtcatttgga ttagctgaaa cgtcttctct ttgtgcttct tcaccttgtg
    11941 tactttcgtc gctttgactt ccagatatat ccttatattg aatctctgtt tctttagttt
    12001 cttcctgatc tcggttaata tttacaggac tagtagtata tgttttttct tgtgcatcaa
    12061 cgttttgtct agcgttagta gatgtatcaa agggataatt aaggtgtttt tctttttcta
    12121 attgattttc taatttttga atctttttat tactctctaa tgccagaact tgctgattct
    12181 caagcaattt tgatctttct tgcaactctt ttctgtaaaa ttcaattgtt gtttcatgac
    12241 gactttcttg tttttcaatt tgattattta aagtattaat ctgattttta agtatttcta
    12301 ctatttgagc atcgctttta gattcaaaat taatattatc cgactctttt ttttgtttgt
    12361 cttcagtagc actttcaaaa ccatatttct ttttattttc atttactctt tgaatgattt
    12421 tttctatatc ggtatcttct ttaataaacg aagtgttttc ctgttttatt gtctctatat
    12481 tcaatctctt aatattatta aaaatcgttt gcttactaac ccctaattct tcactcaacc
    12541 tttttacact tttcatatca tcaacccttt ataaaatgtt tataaaccct tagtaaaata
    12601 ttataagctt ataacgatta ataataaagt aatcgacaaa taaaaatata aattcgccaa
    12661 cctttttatc aagggtttgt aaagggttta tggtgtttat caaaggttta taaacctata
    12721 ttttcccttt tttaagttta ttcaaagtgc tataataacc ttaatataac aaaaacccca
    12781 actactggta atagtcgggg tttggtaata aaacgtttag aaactgttta tttatataat
    12841 tttcgatact tagattatat aatgaataat atcttagtgc aagcacctag attattaaat
    12901 aaacacaaaa aatgtaagcg ttttattacc tcttaccaaa ctaaaattcg gaaagaggtt
    12961 tttattatgt caattaaact ttacaacata aatgaggaat acagagaaaa gttttatcaa
    13021 ataccgaaag tattttttac aaatcctaaa tatatcaagt tatccaatga tgcaaaaatg
    13081 gcatggggta ttcttagaga tcgtttagat ttatcaatta aaaatggttg ggttgattct
    13141 aaaacaggta atatttattt ttattacaaa aatgaaaacc cacaaaatat attaaattgt
    13201 aaaaaagata aagtaattaa aataaagaaa gaacttaatt gaagccgaat tactactaca
    13261 agaaagacaa ggtttaaata aaacaaataa actttattta gttaaaccag aagtaacaga
    13321 agatgatatt tataaaattc aaaagcaaga aaacactgtt gagagcagca atggcaagga
    13381 agtcggaaaa accgacttca agaagtcgat aaaacagaat ctaatgatac tgaacttagc
    13441 tatactgaat ctaacaatac tgaaataaat gatatgaatg acttgaatga tatagagtcc
    13501 gaaaatgaaa ttagtaatca ttcaagtcaa tgcaaggtta tttaatgcaa acaattttaa
    13561 ctgaatttga agaaacacat gcactttata tgcgccgtaa aaacatgaaa caatataata
    13621 tctttaatca atcattcaaa tgattttata acaatcaaag atacatcaaa atatttcgtt
    13681 aaatcatctg actttttaac tgatagcgat gaaaaaatca agaaattgtc agtgatctag
    13741 aaaaagattt gtatcgaaaa cgtatgttaa gttatggtgg attgcttaaa taaaaacata
    13801 aaattttaaa cttagacgat gctgaagatg gcaatttaat taatacaagt gatgaagata
    13861 aaatagcaaa tgaagaagaa aaggcacatt cgattacggc aattttgaaa agcaaaatta
    13921 ttatttaagg cattagtgat aactgatgtc tttttttatt acttttttgt aaaaaaagtg
    13981 ctgtcttatt tgtgtgacaa atgctgtatg tagtgtcaca aaagtgtgac acttgagctt
    14041 tttatgaccc cacacaaaaa cattttcgaa gccttgaaat atctggcttt gccagatccc
    14101 ccattcattg atggggacaa atttccctta tgctcttacg gagattttag agttaaatta
    14161 aaatttctaa gtttttgata agaaaagccc tgcaggaatt tagaagaacg tggggaagtt
    14221 ttaaagaatt ttatgctaat ttttaatttg catgtaaatg ggcagtgtct aaaaaatagt
    14281 tattgttttt gctcaaattt ttgtttttga aaatagcata tatttatttg gctcatattt
    14341 gcgttttaaa agcgtatata agttttagat aaaactatat gaatcacccc taaatcttta
    14401 aaatgccctc ttaaaattca aaataagggc ataaaaaata aaagcccccc cagataaagt
    14461 gaggtagctt tttctttcga cgccgtatcg tcgatgtaat tcaaaaaagt tatgggctat
    14521 aaatctacat cactttttca agaatgtagt agcaatattc aacgcaaatt aatttttata
    14581 actgctcgga atatctcaag ccgtttcttt aattttgaaa taaaaaaatc gacgaaggtc
    14641 gattacgttt ttgcacatgt cgatttatcc gacgtataag tgcgccctta cgggatttaa
    14701 ctagattatg acgacgaatt ttagacctgt aaagcaataa caattaggtc taccaggtag
    14761 ataaagtgta caaagtagaa ccgaaaaagt tatttatttt gaagattttt cgtagatata
    14821 atttgctaat aaacttccta aaactgaata aagaaaaata gttaaactca tgtaatcact
    14881 ccataattct gaagtgtctc catgtctacg catagacacg catttatgcc gagaaaattt
    14941 attgtgcgtt gagaagaacc cttaactaaa cttgcagacg aatgtcggca tagcgtgagc
    15001 tattaagccg accattcgac aagttttggg attgttaagg gttccgaggc tcaacgtcaa
    15061 taaagcaatt ggaataaagc acctatgatt atttaaaaat ataagttata atacattcaa
    15121 aaatattttt gaatgaggtg tattatgatt caaacggttg ttgctgctgc tgttctttat
    15181 attgctacag cagttgattt attagtaatt ttattaatat tttttgctag agcaaagact
    15241 agaaaagaat atagaaatat ttatgttggt caatatttag ggtctattat tttaatatta
    15301 gttagtttgt ttttagcttt tgtattaaat tatgttccag aaaagtggat attaggttta
    15361 ttgggtttaa taccaattta tcttggaatt aaagtggcta tttatgatga ttgtgaagga
    15421 gaaaagagag ctaaaaaaga attgaatgaa aaaggattgt ctaaattagt tggtacggtt
    15481 gcaattgtta cgatagcaag ttgtggcgcc gataatattg gtttatttgt tccttatttt
    15541 gtgacattaa gtgttactaa tttattactt actctgtttg tctttttaat tttaattttc
    15601 ttcttggtgt ttactgcaca aaaattagct aatatcccag gaattggaga aattgttgag
    15661 aaatttagtc gttggattat ggctatcatt tatatagctt taggtttatt tattattatt
    15721 gaaaatgaca ctattcaaac aattttagga tttatatttt aatttagggt gtgattttat
    15781 atgagttatg aaaatacttg tgatgtgatc tgtgtacatg aggataaagt taacaatgct
    15841 ttaagttttt tagaagatga taaatctaag aaattactta acattttaga aaaaatttgt
    15901 gatgagaaga aattgaaaat catattatct ttgattaaag aagatgagtt gtgtgtttgt
    15961 gatatttctt tgatattgaa aatgagtgtt gcttcaactt cacatcattt aaggctttta
    16021 tataaaaatg aggtacttga tttttataaa gatggaaaga tggcatatta ttttattaaa
    16081 gacgatgaaa taagagagtt tttctctaaa aatcaggagg gtttttgaaa cgagtgaaac
    16141 gagtttcttc ttgtcttgat actataaaaa acaaagttat ataacataag acgacttatc
    16201 ggaagttatc tatcatcgta tattcggaga tgatataata tacaggtcgc ctatctctca
    16261 ggcgtcaatt cagacgcaga gaggaggtgc attactttgc taatcatttt cgttcacatc
    16321 atagcaccag tcatcagtgg ctgtgctgtt gcgtattata cttattggct tagtaaacgc
    16381 aacaaataat taataggcga ctttataagc cagcatcgaa aaaatcccca cactattgca
    16441 gtagtgtggg gattggtgca ttaattgtta atcattttcg ttatctagat tataacactg
    16501 gttagtagag gaatgcaaaa agataatatt ttaagtagtc tttttggata ttatcatatg
    16561 atatatttat gtaaattgat tttttattaa aggagcatat atgagtaata ctaaaattaa
    16621 atatcaaatt tttatttcat caacttattt agatttaata gaagagcgtc aagcagtagt
    16681 agaagcagtt ttaaacagta ataatattcc agcaggtatg gaacttttta actcaagtag
    16741 taaaagccaa tgggaaataa taacacaatg gatcaatgaa tcagatgcag tgatattatt
    16801 gttaggttct agttatggaa caattgatag tgaagaaaat attagctata cagaaatgga
    16861 gtacaatttt gctaaatctt taggtaaacc tatatatgtt tttgagatga ataaagcatc
    16921 taccgaatcc gcggaaaagt ctaaaaaatt agatgagttt aagacaaaag ttaaagaaaa
    16981 tagactaatt agtccaaata ttaatgatat ttcggaatta aaaagtaaag tatctcagac
    17041 tttaatacaa agtgtaccta gtgaaattaa agaaaatagt ggatggataa gagtacagga
    17101 ttgtggtaaa aatgtaaaaa aataaaaaaa ttggatgcta tacaaagaga tttagataca
    17161 tatgattttt tgaaagaaga actttataaa atattcatgt atgtaaaaac tttttatttc
    17221 cagcacacac catttgatga tagttatatt gaagatatag aaaactacct gctaaattat
    17281 gaaaataatc cacataaatc attcatgaaa ataaaagttt atgattataa aaacttttta
    17341 aaacatttaa aaaacatatt gattttaatt aaacaaggaa aaaagccatt agctcaagcc
    17401 ggtcatttta aattaagctt ttacgaagag gatttaaaat tgttacaaaa aaattataag
    17461 gctacgctag tcgatattaa aggattgaat aaacattttt ataaggaatg tgataggtgc
    17521 ttaaaagcat ataatatttt tattgagggt ataaataaac atttatatga atgatataag
    17581 tacaccctaa tcatacatat ataattgttg tcactttact cgatatatga gaatgacttt
    17641 acctaataaa tgagaaaagc aacgcactat attaaaatgc gttgcttttt ctattatcga
    17701 atacctataa ctaaactatc tctagctgtt tctacaactt ctgtacttat tttatctaaa
    17761 ccatttatat ccataattct gtctatctgc gcaaataaac gatgaatcag cctaaaattc
    17821 ccttttgtaa ttttaataat cgtcgttatt gcctcataat cagtaaagtc ttcgagtttt
    17881 aaatcaaatc ctaagtcttg acacttatat tctaatatat gatgcgtctc atctttactt
    17941 agattatcaa attcatgcgc aaaacctata cgcgaatata gttgaggata acgagatagt
    18001 tttttctcta tacccggcat accaataaat atcattgcta aattatgttc atcataaata
    18061 gctcttaatt gctctaaatg ttgcacttta aggcgatcaa tctcatctac tataattaaa
    18121 tcaatatatt taaacatatc actataatgt ttactatgtt catgtccata cttatttaca
    18181 acatgtaatt tttgacccaa atcaatgatt gcagtaatac tatataaatc attccttaat
    18241 ttagtttgtt tttctgctgg tgcattataa aatattttat ccaccgatag tatgtcatcg
    18301 gtcgttttac tagctaaatc ttcccaacct ctatggttaa cttgtttttc aatagtatcc
    18361 caatttgtgt aatacctaga cgataaagtt tttccgacac caggttgacc ataacatata
    18421 ccaatatact gatatttaat acaagcatca caaaattcag caaagcgttt atactctttc
    18481 gtttcaatga aattttgatt tttagtcatt acgatatctc tttaattttt tgacgttttt
    18541 agtcgtttct tgaggtgaat aaccacaatt tttttcttcc ttaattaatt ccgtggtact
    18601 tggactagca atattttgtt ttaagtgttt tcttctctga ctacgagcgt gctgtatgtc
    18661 ttttatatct atggagtaat cagctatttc tggacatata gcagtacaaa gaaattcatc
    18721 tctataaaac acacgtattt cagccatatc atttgggtta taacgaatca gtacatattc
    18781 acctacataa gcttctagat tagtattact gtatcgaaaa ccttgaaaat gaatgccatc
    18841 tgaatgaact tttctagatt taggtatttc caacaataat aaatcaagtt gttctaagct
    18901 acttggcata ttagggaaaa agtgattact attccaatga ctgataggcg tggtatgaat
    18961 agtactatgt tctttttgat tataatcttc aattaaaaag taacgtaatt tttcttcaaa
    19021 attgtgaaaa tttattagat tactaggagt atcattattg tttatatatc caggcagttg
    19081 ttctaagaaa gtttgattaa ctgtctgaaa gaatcgttct atttttccac gaccacgagg
    19141 aacaccaact ttcgaaaaca tcaagttaat tttcaaatca atagcgactt gttccatatg
    19201 atgtgaagta aagtcacttc catgatctgt gtagaatttt tcgggtatac cacacactgg
    19261 ccaattggta tcatttttat tccatattgc ttggtgtagt gttaatgctg tgttttgagc
    19321 attggggggc atcaaaactt aagaaatagc ctgcaattgc acgactatag tcatccataa
    19381 taatagttag ccatggtcta ttgatattac ctttttggtc taatataaag atatctagca
    19441 aagtatggtc tgcttgccat atttcattgg gtctcgagga ttctctaatt tgtattaaat
    19501 cgtacttatt ttgatagtat ttttcaccct gatgagagaa atcaataaca gacttaggca
    19561 tcgctttaat aacactataa atttgtttat aacttggtat atcaaaatta ttttctttac
    19621 agtaattagt gattttccta tgaattgtgg ctatactatt tcttttattc ataagcgcaa
    19681 ggcgttcaat ttcatcaatt atctttggtt ctattttacg tgttccttta tcagctcttg
    19741 tcgcatagat tagtccttta agtccttgtt ctttatatga cttattccac ctataaaggg
    19801 tgcttaacgc aatcccttta cttttagaaa tacttgataa agattgttcg cctaggataa
    19861 aaggttttat aatattgtac ttttgagtag cttttttacg tttggcttca gaaaaattag
    19921 ttagatactt ctccttgttt ttcatcttcc ttgtcctcca atttattgag gtaacgataa
    19981 attgttgtac gtgaaacatg ccactgttct gcaattgtct taattggcgt gccattatca
    20041 taaagggtct taagtaagtt taaatctttt tgatttaatt tttctggtct gcctccatat
    20101 cggcctctag cacgtgctgc aattcggcca gctgaagaac gctctaaaat taaattacgt
    20161 tcaaattctg caaatgctgc gaataaatga aataataatt gtccagttga acttgattta
    20221 tccattgtta tattttcttc taaactatgg aaacttacac ctcgttcgtt aagctcatta
    20281 actaatgtga ttaaatcctc catattacgt cccaatctat ctaatctcca aacaacaatc
    20341 gtatctcctg acctcgcaaa ttcaattgct ttatctaaac caggtctttt actttttgaa
    20401 ccactcatat ggtcattaaa tatcttttca caaccatatg tatttaatcg atcctcctgt
    20461 aaatttaaat tttgtaatcc agttgaaact cttgcataac ctattttcaa aatacaaacc
    20521 tcctatatac atcttcagtg taccataact taattacaag gtcttttatg aacatagatt
    20581 ttttgttggg tttttgaaca cataatccta tgtaattctt acttatttag acataaaaga
    20641 agagtgtaca gaaaatggtt attttctgta cactctcatc tttaataata tattttaaat
    20701 gtcattaaca aaactaattt aataagagtc aagcatagtt tacatatgta ttatgtttga
    20761 aacatacctc taattacttt ttactaatat catttaaaat atctcgcaat tcttcaattt
    20821 ctttgttatt taattcttca tttttcgcaa aattcaacac taaacttttc atatcccctc
    20881 catacagttt attaagaaag gttttagcag ttttcatttt aatatcgtct tctttaatat
    20941 ttgatgagta aaaataaata ttttctgatt tgtatcgttt tataatctct tttttatata
    21001 gtcttgtgat taatgttcta atcgttttat cactaacttc tttatatttt tgaatttcaa
    21061 ctacaatttc attagctgat actgattttt tatcccatat tatattcata acatcccatt
    21121 cagccataga tatttcaact tgcttattgg ccatttaaaa cacccattcc ttttaatatt
    21181 ttttcactta ttaattcagc atttttccca gacgggtttc catctgataa atgtgtagca
    21241 aaataatact tatcatgatt tgtaattacg taacctacaa accacccatt attatacttc
    21301 ccgtttacta tacctgtacc tgttttccca tacagttcat acttttcatt tttcttaatc
    21361 aataatgaag aagataattg attctttgct tttttactaa aatggttatt ttgttccatc
    21421 atatttttaa aaactattac ttgttcaaga ttagatattt tcaaactatc ttccatccaa
    21481 tagcttttat aacttcccaa attttcatta ccataattta attgcttgag ttgagccgca
    21541 gtatagttct ttggtatttg attactaata cgttcgaagt accaattaac tgaattttgc
    21601 attgctgtat ttaaatcttg ttccttattc caagactcga aaagataatg tttatgattc
    21661 catgacatac gtgaattttt atcacttata atatgacggt caagtccaaa cagtgctaaa
    21721 taaattttgt aagttgaatc aggagaatat ctttttctac tttctttttc attataaata
    21781 tagtatttgt ctttcttcat gctgtacata acgaaagatc cactatttga accaaaattt
    21841 ttactttcat ctaaaatttg gtaatcgctt tgtaagggct ttttataatt ataatcagtt
    21901 aaggattgcc ccattaaaaa ctggctttga attatcatga taaaaaaagt aaaaatacaa
    21961 ataaaaatta gaattaattt tgactgcttt ttcagatcgg cttcttttat attaattaat
    22021 ctatttttga gtaatgattt tttaccatta aatgaatggc ttaatatatt tttgttgaaa
    22081 aatgaggttt ttaatacgga gtccataata gattccgcat aagttttaaa ttcatttttg
    22141 ttaatattat taaggacaaa tctatcggct tctacttcat tgtcatgaat tatctttctt
    22201 tttacaatat gtactaatgg attgtaactc ataataatag agaagatatt aaagataatt
    22261 aaatgtaaag tatctctatt tttagcatga gcatattcat gcagtattat atattttagt
    22321 cttttatcaa ttacactttt aaaatatgaa ctaggaatca aaataatata tttcccatac
    22381 caaaaagtta ttggagattg aatagcctct gcttttcgaa tcacaatatt ttttttatat
    22441 tgatggttga aaagtatcgt atctatttta tttttttcat tttcgtttag ataaagtgac
    22501 tgtttcttta aatatttaag atataataag gctttcaaaa atttaaaact taaaataata
    22561 actaaaacta tccaaataac tgtgcagata ttatcaattg aatcccaatt aaacttatgg
    22621 atatctgttg cgaactcttg aacaggtttg gtggtaatta tgttatggtt caagtcgtgt
    22681 gacttacttt caactgtggg cgcttgatta ttcacattat taaattttat aaaagagaat
    22741 ttaattggga taaaaggaat taatccagca agcacagtta gataccaaac tttataattt
    22801 aaactataat taaaatagcg ttttaatatg tacctaaaaa agactaacaa tagaaatata
    22861 aaacaaaagc ttactacact cattattagt aatttagcca ttttgacacc ttctttcaaa
    22921 tatttataat aaacaattga catcaatatt acaattgtaa tattattgtt ttgtaaaaat
    22981 tacaactgta atatcggagg gtttattttg aaaaagttaa tatttttaat tgtaatggct
    23041 ttagttttaa gtgcatgtaa ttcaaacagt tcacatgcca aagagttaaa tgatttagaa
    23101 aaaaaatata atgctcatat tggtgtttat gctttagata ctaaaagtgg taaggaagta
    23161 aaatttaatt cagataagag atttgcctat gcttcgactt caaaagcgat aaatagtgct
    23221 attttgttag aacaagtacc ttataataag ttaaataaaa aagtacatat taacaaagat
    23281 gatatagttg cttattctcc tattttagaa aaatatgtag gaaaagacat cactttaaaa
    23341 gaacttattg aggcttcaat gacatatagt gataatacag caaacaataa aattataaaa
    23401 gaaatcggtg gaatcaaaaa agttaaacaa cgtctaaaag aactaggaga taaagtaaca
    23461 aatccagtta gatatgagat agaattaaat tactattcgc caaagagcaa aaaagatact
    23521 tcaacgcctg ctgctttcgg caagacttta aataaactta tcgcaaatgg aaaattaagc
    23581 aaaaaaaata aaaatttctt acttgattta atgtttaata ataaaaacgg agacacttta
    23641 attaaagatg gtgttccaaa agactataag gttgctgata aaagtggtca agcaataaca
    23701 tatgcttcta gaaatgatgt tgcttttgtt tatcctaagg gccaatctga acctattgtt
    23761 ttagtcattt ttacgaataa agacaataaa agtgataagc caaatgataa gttgataagt
    23821 gaaaccgcca agagtgtaat gaaggaattt taatattcta aatgcataat aaatactgat
    23881 aacatcttat agtttgtatt atattttcta ttatcgttga catgtataat tttgatatca
    23941 aaacctgatt ttccctttat tattttcgag atttattttc ttaattctct ttaacaaact
    24001 agaaatattg tatatacaaa aaatcataaa taatagatga atagtttaat tataggtgtt
    24061 catcaatcga aaaagcaacg tatcttattt aaagtgcgtt gcttttttct catttataag
    24121 gttaaataat tctcatatat caagcaaagt gacataattg ttgaatttat agtaactgaa
    24181 acgtatgatt agggtgtata ttaattatga gatggaaatc aatgattgtt ggctatgcga
    24241 gagtatcatc gaacgatcaa aatttagaaa gacaacttga aaatttaaag acgttttgtg
    24301 ctgaaaaaat atttacagaa aaacagtctg ggaaatctat tgaaaataga ccagtacttc
    24361 aagaagcact taactttttg aggatgggag atcggtttgt agtagaatcc attgattgtt
    24421 taggtcgtaa ttatgatgaa gtggttggca atccattact ggataagttt atgaaagatt
    24481 taattattca aatattagca atggtttcag aacaagagag aaatgaaagt aaacgtcgac
    24541 aagcgcaagg aattcaagtt gcgaaggaaa aaggcgtata taaagggcgc cccttacttt
    24601 attcaccaaa tgctaaagat ccacagaaac gtattattta tcatcgcgtt gtagaaatgt
    24661 tagaagaagg ccaatcaatt agtaaaattg cgaaagaagt aaatataaca aggcagacga
    24721 tttacagaat taaaaacgat aatgatttga tttaataaac tttgtttcaa tgcaactgag
    24781 agcttctcac agcgtctcaa aacagatgat tataatctat tatgagtttt taataataag
    24841 acaagaaaag agagcgtaaa tatgctctct ctttttctca ttaacgaacg taatttagaa
    24901 ataatattta ttggtatttc aagaatatat atataattat ttactaagtc catttaaaaa
    24961 aatttgagag aacttgttca taagtttaat tctttcatta atattttgtt catgtgtaaa
    25021 ggtaacaatt ccattaacag cgtttgctgc tattttacta acagcattaa catcattaat
    25081 acaccattcg ccatttaaat taccctcctt aaaaattaca tgataagcat ctatatattt
    25141 gttttctaat ttattcattt tttcattaat gctattagtt ttataatatt cagtgtaaaa
    25201 ttcaattatt gcgttttgaa gtggataata gtattcggtt gttaaagata gttcattata
    25261 taaataaaat ttttctctat tagttttaca tttgatttgt tcctttttcc actgttcttg
    25321 ccatttagat tcttctatat ttaaaatttc taaaaataga ttttcttttg ttttaaagtg
    25381 ataataaaga ttccctttac tactttctga taatttaaca atttctccag tagtagtggc
    25441 attatatcca ttttttataa ataattcctt tgcgacacct agtattttat ctttcaagtt
    25501 catcacgcct ttaatgagta ttaagtacta tcaatgatag cacaaaaaat tcaaagaata
    25561 attgtataac acaatacagc catttaaatt tcgcaagatt ttttgttgta atatgtaaaa
    25621 aaaatagatt ataatcctta tagaccgatc gcacggtcta taaggattgg agggaactta
    25681 aatgatttca ttttttacaa aaactactga tatgatgaca tcaaaaaaaa gatggactgc
    25741 actagtagta ttagctgtta gtttgtttgt tgttacaatg gatatgacaa tattaattat
    25801 ggctttaccg gaattagtaa gagagttaga gccttctggt acccaacagt tatggatagt
    25861 tgatatatac tctcttgttt tagctggctt tataattcca ttgagtgcct ttgctgataa
    25921 atggggaaga aaaaaagcat tattaactgg atttgcttta tttggcctcg tttcattagc
    25981 tatatttttc gcagaaagtg cagagttcgt aatagctatt cgatttttac ttggtattgc
    26041 aggtgcttta ataatgccaa ctactctttc aatgataaga gtaatttttg aaaaccctaa
    26101 agaaagggcc actgcattag ctgtatggtc aatcgcttca tcgataggtg ctgtttttgg
    26161 accaattatc ggaggagctt tacttgagca attttcatgg cactcggcat ttttaattaa
    26221 tgtaccgttt gcgataatag cagttgtagc aggtttattt ttattaccag agtctaagtt
    26281 atcaaaagaa aagtctcact cgtgggatat tccttctaca attttatcaa ttgcaggcat
    26341 gattggactg gtatggagta tcaaagaatt ttcaaaagaa ggactagcag atattattcc
    26401 atgggttgta atagtattag caattaccat gatagtgata tttgttaaac gtaatttatc
    26461 aagttctgat ccaatgttag acgtaagact ttttaaaaag agatcatttt cagctggtac
    26521 aattgctgca tttatgacaa tgtttgcaat ggcatctgtt ttgttattag cttcacaatg
    26581 gttacaggtt gtggaagaac tttctccttt taaagctggc ttatacctat tacctatggc
    26641 aataggagat atggtgtttg caccaattgc acccggatta gcggcgcgat ttggaccgaa
    26701 aatagtgtta ccttccggaa ttggaattgc agccattggc atgtttatta tgtatttctt
    26761 tggtcatcca ttatcatatt ctacaatggc tttagcatta attttagttg gagctggtat
    26821 ggcttcacta gcagttgcat ctgctctaat aatgttagaa acacctacat caaaagcagg
    26881 taatgcagct gctgttgaag agtctatgta tgaccttgga aatgtttttg gtgtagcagt
    26941 acttggtagc ctatcttcta tgctttatcg tgtattttta gatatttcat ctttttcatc
    27001 aaaaggtata gttggagatt tagctcatgt agctgaagaa tctgtagtgg gcgctgtcga
    27061 agtagctaaa gctacgggga taaaacagct tgcaaacgag gctgtaacat catttaatga
    27121 tgcttttgta gcaactgctt tagtaggtgg gattatcatg attatcattt caatagttgt
    27181 ctatttgtta attcccaaat cacttgatat aactaaacaa aaatagtact aaacatatat
    27241 ttttagatat tgtagattag tttattttta aaattagtaa tagttaaaaa gtagaaccat
    27301 taattttaaa tggttctact tttttattat taagtaaaat tataaaagac aatttcttaa
    27361 attaataata tgatatacct atatctaata atttattagt attaataaac cttgaaagga
    27421 tgattaactg atgaatagag cagatatttt taaaatattt gatgtagaac ttaatataac
    27481 tatattttta ggagcactag tagcaacttt catctatttt ataagttaca tgaacaatgt
    27541 aaatgaatta attacattat gcttaacatt agcttttcct gcatatttat taaaaattag
    27601 cagatcgttc gaggttattt tattagagga aaaaataaaa aatagtaaaa tcaaaatctg
    27661 tgacaaagat agaattgaag gttctgttgc aaagttaaaa atcatgtaat atttatactg
    27721 aaaaacgaaa aataattaat actaacaaaa ctctttactg ttaaaacaat atttgctttt
    27781 atagttttgt atagatgtgc atataatttt aactttgcaa cagaaccaaa tttataatag
    27841 tatagtttca acatgggatg tttataaaaa taacgaatat agcaaagacc tttcaaga
//
LOCUS       DAMZKC010000018        27642 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.018, whole genome shotgun sequence.
ACCESSION   DAMZKC010000018 DAMZKC010000000
VERSION     DAMZKC010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 27642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27642
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.018"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..218
                     /locus_tag="POB55_000539"
     CDS             <1..218
                     /locus_tag="POB55_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDI6499525.1"
                     /translation="DFTLMPMGLNILLVLLYFVYLGLTIVMIVFLCFDSKPANKYGES
                     PKYPSTIKNQPVTSETPKTPEETDNQL"
     gene            449..1348
                     /locus_tag="POB55_000540"
     CDS             449..1348
                     /locus_tag="POB55_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6499526.1"
                     /translation="MGLVIEHVTKRFGKMTAVNDISLELESGKMLGFLGRNGAGKTTT
                     FRMILGLSEPTEGHITYNGKKLDKTMYNRIGYLPEERGLHGKLTVEEELKYLATLKGM
                     SKTEIQQQISYWLERFDITENRKKRIDSLSKGNQQKIQLLASMLHKPELLILDEPFSG
                     LDPVNVELLKEAVKDLNDWGSTIVYSSHRMEHVEELCDDVCILDKGQLVVSGDINHVR
                     ASNGNKKVVIESETTLPDLTNIRGIIHSENMKQGLQLTIENEDVAKDIYQVVAHQGYV
                     KRFQVVEPSLQDIFIEKVGGKDA"
     gene            1341..2573
                     /locus_tag="POB55_000541"
     CDS             1341..2573
                     /locus_tag="POB55_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6499527.1"
                     /translation="MHKFWATFTLTYIKKIKAKSFVIFMIIIAALMIGLSNIDKIINM
                     FDDGPDKIGVAAPNEQIYKVFKQQANTFHSDAKFTKVSIEDAEKEVKKHKLDKAYIIK
                     VNQNRTLQGTIISEKRVSHEDSQKVQALLTAIQTNMVAGELNINKEDLQKLQAQSKVD
                     NKVISNDEVDKVSEGQKIFNYALAYGIIFLMFFIVLNYASQIAMEIASEKTSRVIEMI
                     ITSISPIQHIFAKILGVIAVAVTQIILIVLMAVICIFAFDLKDLLQGFNVEMNQLSWQ
                     IIIVGIISSIVGIMAYVLLAAILGSLTSRIEDLNQSLMPLTLLGMIAFYIAIFTINNP
                     DGRLALITSYIPFLAPFQLVVRAQTSGLQIYEVILSSLISIVMVAVLIWIAIKTYKDS
                     VLTFERGLFNSMKRVFKK"
     gene            2557..2742
                     /locus_tag="POB55_000542"
     CDS             2557..2742
                     /locus_tag="POB55_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499528.1"
                     /translation="MYLKNKIDESDTHLSYKMMMKACYSYFFIFSYRIMSKKVYNMNV
                     KTNFLKWECEVRRNVNK"
     gene            2791..3117
                     /locus_tag="POB55_000543"
     CDS             2791..3117
                     /locus_tag="POB55_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HDI6499529.1"
                     /translation="MLYPIFIFILAGLCEIGGGYLIWLWLREGQSSLVGLIGGAILML
                     YGVIATFQSFPSFGRVYAAYGGVFIIMSLIFAMVVDKQMPDKYDVIGAIICIVGVLVM
                     LLPSRA"
     gene            3147..4166
                     /locus_tag="POB55_000544"
     CDS             3147..4166
                     /locus_tag="POB55_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDI6499530.1"
                     /translation="MIVEVEHQRQSVDLIKIDNDETKIIFTNYGARVVSWKYHDNNIV
                     LGNVVEADEFYHSNPFKFGASIGRYSGRIDNAKFKLKGKEYQLEKNNGEHHLHGGCHG
                     LDNKLFDYEIRNEIAQIKVIFKTVLKSADDHFPGDIDVTITHIYDADHQWSIEYEAVA
                     SEDTLFSPTNHVYFNLNRDNNVVDNHRISSNQLDMYVLDERNIVTGDILDLHEVFEDN
                     KIKLSDIFTSQHAQLSQQMTRFGGLDHPFTVGEHKMYVENHEFMLEVDTDMPHVVFFT
                     FNQPDEWDSPFNIYKPHSGFTLETQFLPNDINLYGEKAPSILKAGEVFNSKTSFRIEE
                     KVEND"
     gene            complement(4402..5055)
                     /locus_tag="POB55_000545"
     CDS             complement(4402..5055)
                     /locus_tag="POB55_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HDI6499531.1"
                     /translation="MISIHAISTGKIQDLPYSSKRPMRSALDKTKISQTTWLSSTGFT
                     GDEQAYKDHGGPHKAVCGFSKHNYALYQDDLPTLPIHAMFGENLTFDYLDESDVYFGN
                     QYRLGEALIEVSEIREPCWKIQAKYNIPDLVKRMSTSGKTGFYFRVLKQGYVSPNDQL
                     YLIQEAPIEHRLSVQQLNELYYNDRQNQDMLRYALNNPFLSPTRRDKLQKMYNRTLK"
     gene            5229..5915
                     /locus_tag="POB55_000546"
     CDS             5229..5915
                     /locus_tag="POB55_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase A"
                     /protein_id="HDI6499532.1"
                     /translation="MKDVKALKLMTLNDVLSQINGDMTLGIGTGSTMELLLPQMAQLI
                     KERGYNITGVCTSNKIAFLAKELGIKICEINDVDHIDLAIDGADEVDPSLNIIKGGGG
                     ALFREKVIDEMASRFVVVVDETKIVQYLGETFKLPVEVDKFNWYHILRKIESYADIKV
                     ERRVNEDVAFITDNGNYILDCKLPKGIDPYKFHEYLIHLTGVFETGYFLDMADQVIVG
                     TQEGVKILEK"
     gene            complement(5981..7240)
                     /gene="sdpC"
                     /locus_tag="POB55_000547"
     CDS             complement(5981..7240)
                     /gene="sdpC"
                     /locus_tag="POB55_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease SdpC"
                     /protein_id="HDI6499533.1"
                     /translation="MKNNKISGFQWAMTIFVFFVITMALSIMLRDFQSIIGVKHFIFE
                     VTDLAPLIAAIICILVFKYKKVQLAGLKFSISLKVIERLLLALILPLIILIIGMYSFN
                     TFADSFILLQSTGLSVPITHILIGHILMAFVVEFGFRSYLQNIVETKMNTFFASIVVG
                     LMYSVFSANTTYGTEFAAYNFLYTFSFSMILGELIRATKGRTIYIATTFHASMTFGLI
                     FLFSEEIGDLFSIKVIAISTAIVAVGYIGLSLIIRGIAYLTTRRNLEELEPNNYLDHV
                     NDDEETNHTEAEKSSSNIKDAEKTGVATASTVGVAKNDTENTVADEPSIHEGTEKTEP
                     QHHIGNQTESNHDEDHDITSESVESAESVKQAPQSDDLTNDSNEDEIEQSLKEPATYK
                     EDRRSSVVIDAEKHIEKTEEQSSDKNK"
     gene            7530..8465
                     /locus_tag="POB55_000548"
     CDS             7530..8465
                     /locus_tag="POB55_000548"
                     /EC_number="3.5.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HDI6499534.1"
                     /translation="MYKQGEPNLWTGRLDSETDPKKFRHFQTVTFEDLSKLEKSSMPS
                     GVGILGYAVDKGVALNKGRIGAKEGPDAIKQAFAGLPDLNQCETLVDYGNVYHDHEEL
                     IDTQKEFAMLAAKSIANHRQTFLLGGGHDIAYAQYLATRKVYPTQSIGVINIDAHFDT
                     RAEQESTSGTSFRQILEEDDNTDYLVLGIAQGGNTQSLFDYAKEKKIDYVFADELLNH
                     VSPPIKDMIERFVHEHDVIMFTICMDVIDSAFAPGVSAPAVLGLYPHTVLELAKRIIP
                     SDKVSSVSIAEMNPTYDADNRTAKLVANLVHHFLK"
     gene            complement(8898..9782)
                     /locus_tag="POB55_000549"
     CDS             complement(8898..9782)
                     /locus_tag="POB55_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI6499535.1"
                     /translation="MKIIQLEYFLAIVKYNSFTKAAQFLHISQPSLTATIKKMEADLG
                     YDLFTRSTKDIKITEKGIQFYRYASELVQQYRSTMEKMYDLSVTSEPRIKIGTLESTN
                     QWIANLIRKHHSDYPEQQYRLYEIHDKHQSIEQLLNFNIHLAITNEKITHEDIKSIPL
                     YEESYILLAPKETFKNQNWVDVENLPLILPNKNSQVRKHLDDYFNRRNIRPNVVVETD
                     RFESAVGFVHLGLGYAIIPRFYYQSFHTSNLEYKKIRPNLGRKIYINYHKKRKHSEQV
                     HTFVQQCQDYLYGLLEAL"
     gene            9942..11603
                     /gene="hutU"
                     /locus_tag="POB55_000550"
     CDS             9942..11603
                     /gene="hutU"
                     /locus_tag="POB55_000550"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HDI6499536.1"
                     /translation="MRKIQAKKGLSIECKGWEQEAVLRMLYNNLDPEVAERPEDLVVY
                     GGIGKAARNWEAFEAIEKTLRELESDETMLVQSGKPVAVFKTHEEAPRVLISNSVLVP
                     EWANWDHFNELDKKGLIMYGQMTAGSWIYIGSQGIVQGTYETFAELGNQHFNGDLAGT
                     VTLTAGLGGMGGAQPLAITMNHGVAICVDVDETRVDKRIDTKYCDVKTADLDEALKLA
                     EEAKERGEGLSIGLVGNAVDIHQAILEKGFKIDIITDQTSAHDPLNGYVPQGYSVEEA
                     KVLREKDPKKYVELSQASMAKHVELMLEFQKRGAVAFDYGNNIRQVAFNNGVKNAFDF
                     PGFVPAYIRPLFCEGKGPFRFAALSGDPKDIERADEEMRKLFPENEKLLRWLDLAEEK
                     ISYQGLPSRIAWLGYGERAKMGLALNRLVRDGEISAPIVIGRDHLDAGSVASPNRETE
                     SMKDGSDAVGDWAVLNALINTAAGGSWISFHHGGGVGMGYSLHAGMVVVADGSERAER
                     RLERVLTTDPGMGVARHVDAGYDIAIQTAKEKGIHIPMIDKAGDK"
     gene            11603..12841
                     /locus_tag="POB55_000551"
     CDS             11603..12841
                     /locus_tag="POB55_000551"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HDI6499537.1"
                     /translation="MNDLIINHIAELILPRSTDKPLKGKELDELNVVKNGTVVIKDGK
                     IVYAGTHTDDYDATETIDASGKVVSPALVDAHTHLTFGGSREHEMSLKRQGKSYLEIL
                     EMGGGILSTVNATRETSEDDLFKKAEHDLLTMIKHGVLAVESKSGYGLDRENELKQLK
                     VSNRLAEKYDLDMKHTFLGPHAVPKEASSNEAFLEEMIALLPEVKQYADFADIFCETG
                     VFTIEQSQHYMQKAKEAGFKVKIHADEIDPLGGLELAIDEQAISADHLVASSDKGKEK
                     LRNSDTVAVLLPATTFYLGKEDYADARGMLDNNGAIALATDYNPGSSVTNNLQLVMAI
                     AALKLKLSPNEVWNAVTVNAAKAIDINAGTINTGDKANLVIWDAPNHEYIPYHFGINH
                     AEKVIKDGKVIVDNTLSFKA"
     gene            13117..14238
                     /locus_tag="POB55_000552"
     CDS             13117..14238
                     /locus_tag="POB55_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 peptidase aminoacylase family protein"
                     /protein_id="HDI6499538.1"
                     /translation="MGLKDQAVTWRRYFHQFPELSDKEFKTTQKIKDILTEHHIRILD
                     LPLATGLVAEVGQGLSCIAVRADIDALPIQELVEQDFKSENEGVMHACGHDIHMASIL
                     ATAVKLKEIEGTLTGRVKFIFQPAEELGHGAFKIIETHALKDVQAVLGFHNDPSRSVG
                     TFAIKTGAITSAVDRFEFHIKGVGGHAAKPEQCNDPVIVLAQLINSIQSIVSRNLSAF
                     DEAVVTIGQISCGNTWNVIADHAYVQGTVRSFDPVVRKLVETRLQDIADGLAQAYNMK
                     INLNYTHLPGAVMNDEALTHKAIAVAQHVGYKAEMMEQPLTIGEDFSGYSQHFPSVFA
                     LIGSHSEYDLHHPQYKPDERILEKVPEYFVEFVKRLLHE"
     gene            14349..15230
                     /locus_tag="POB55_000553"
     CDS             14349..15230
                     /locus_tag="POB55_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDI6499539.1"
                     /translation="MAAQDPRTKFKTTDYEKQEQEVPGLQSEMTPAPDCGETSYQGHQ
                     RLQGYKILVTGGDSAIGRAAAIAYAKEGADVAINYLPSEEQDAQEVRQVIEESGQKAV
                     LIPGDIRDEQFNYDLVEQAYQQLGGLDNVTLVAGHQQYHDDIHGFTTEAFTETFETNV
                     YPLFWTVQKALEYLKPGASITTTSSVQGYHPSPILHDYAASKAAIISLTKSFSEELGP
                     KGIRVNCVAPGPFWSPLQISGGQPQSKIPTFGQKTPLGRAGQPVELCGTYVLLASEES
                     SYTTGQVFGVSGGVQID"
     gene            15717..16286
                     /locus_tag="POB55_000554"
     CDS             15717..16286
                     /locus_tag="POB55_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499540.1"
                     /translation="MALIVSRAILFLILALAFGIATVFPEQKLPLYIVFTLFVAVAIV
                     SNVWFTHQNVKKHIIKFNKYVAYFLSFVSGLLYIGFVIYTLTIGSYDVLPIIIFAISI
                     VLVQILENILGISIKNTRLSNKQLKVISKQRVQQFKVISLILVSVSIVFIFIALMLKW
                     WLLLVISIIVLIIITVAMIVYEKYYTTPD"
     regulatory      16339..16434
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            16511..17824
                     /locus_tag="POB55_000555"
     CDS             16511..17824
                     /locus_tag="POB55_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020444780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HDI6499541.1"
                     /translation="MEVHKKSNAWALFPLLLFVALFLGVGIITGDFTSMPLNVAITIT
                     VIVALLMNRKESFAKKVEVFTKGAGHSNIILMMLIFILAGAFSNTAEKMGGVKSTVNL
                     GLSLIPENLIIVGLFVICMFVSISMGTSVGTVAAIAPVGYGFAQATDVPTALAMATVV
                     GGAMFGDNLSMISDTTIAAVRTQHTKMKDKFRVNFKIVLPGAILTIIILYFLTNGISL
                     NHAKSYDYNLIKVVPYILVLVLALLGVNVIIVLIGGTLLAGIIGLIDGSFGWMGLLDA
                     VSKGIISMEDIAMIALLIGGLVGIIQHNGGIEWLLQFVRSKVKSKRGAELGIASLVSV
                     ADIATANNTISIIMSGPLAKNIADEYDVDSRKSASILDIFGGCFQGLLPYSPQVISAA
                     GVAGISPLMLFPYSIYPILLGVCGLIAILFNFPKLKKNTSQEMKH"
     gene            17991..18497
                     /locus_tag="POB55_000556"
     CDS             17991..18497
                     /locus_tag="POB55_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HDI6499542.1"
                     /translation="MAKFNVENEHVEVEVEKLYKFSPELVYEAWTKKDLLKQWFMTSA
                     RTNKEIEADVKEGGKYRIVDQQRNGKVNVIEGIYESLVMDEYVKMTIGMPGLSETQDV
                     IEVEFFERETGGTQMLFYYRSLVEKERRFTNLEYKQKKKEYHDAMVHGFELMFDKMYH
                     VIETSTQQ"
     gene            complement(18555..19319)
                     /locus_tag="POB55_000557"
     CDS             complement(18555..19319)
                     /locus_tag="POB55_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDI6499543.1"
                     /translation="MFLDEHINRNFDKLNDNDLHIAHFINTHIDECKNMKIQDLAQFT
                     HASNATIHRFTRKLGFDGYSDFKSYLKFESNKTHQLPSNSIDSFKQEIDSTFSYLERV
                     DYQLLTEKMHTASTIYLYGTGRAQLNVASEAQRILLTLHNHVIVLHDEHELKMILNKS
                     EPNDLFFIISLSGETHELLEVTNLLQLRQKYFISVTTMKDNTLAQKANYNVYVSSNTF
                     YLTDGTDYSSFISYHIFFETLVRKYNEYLLHNDISN"
     gene            19649..21253
                     /locus_tag="POB55_000558"
     CDS             19649..21253
                     /locus_tag="POB55_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDI6499544.1"
                     /translation="MNAIKRFGSAMIVPVLMFAFFGIVLGFATLFKNPTIMGSLADQH
                     TFWFKFWSVIESGGWVIFTHMEVVFVVGLPLSLAKKAPGHAALAALMGYLMFNTFINA
                     ILTQWPHTFGANLEKGVENVPGLKSIAGIATLDTNILGGIIISAIITWIHNRYYSKRL
                     PEMVGVFQGLTFVVTISFFVMLPLAAITCVIWPTVQHGIGSMQHFIIASGYIGVWLYH
                     FLERVLIPTGLHHFIYAPIEVGPVVVNHGLKAEWLQHLNEFAKSTKPLKEQFPYGFML
                     QGNGKVFGCLGIALAMYATTPKENRKKVAALLIPATLTAVVVGITEPLEFTFLFIAPY
                     LFVLHAVLAASMDTLMYAFGVVGNMGGGLLDFISTNWLPLGKEHWGTYVAQVIIGLIF
                     VAIYFFLFRFLILKFDIPLPGRKKTEEEVKLFSKQDYKNKKGDSVDSKRASTGNEYED
                     KAAYYLDGLGGKENIKDVTNCTTRLRLTVYDESKVADTEYFTHQQMAHGLVKSGKSIQ
                     VVVGMTVPQVREAFEQMVEDQSSEDK"
     gene            complement(21319..21849)
                     /locus_tag="POB55_000559"
     CDS             complement(21319..21849)
                     /locus_tag="POB55_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499545.1"
                     /translation="MLIDKFETYIINIAGLNDRTTRKKLSKLCKSVQFCDALQFSINK
                     QFNQYVLEISLPKQQLPYFISFLSFHQYSIFQVLSPKKINELLDSDNLYQSAKRFDIN
                     IDGLQDAFIKDKVIDIMNMFQNHTDITYTLNKSHAHIICTPEIFAKLLHTIATRNIDI
                     LSANYRSSSMSKARIS"
     gene            22117..23034
                     /locus_tag="POB55_000560"
     CDS             22117..23034
                     /locus_tag="POB55_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HDI6499546.1"
                     /translation="MFQKLSIFATKSFLVWMLVAAVIGFISPQHVATLGKWVPYLLGI
                     VMLGMGLTITPNDFKMVFKAPRAVIIGVCLQFSIMPTLAFIIAKSFHLPPDIAVGVIL
                     VGCCPGGTSSNVMSYLAKANVALSVSITTVSTLLAPFVTPALIYLFANEWLEVSFLSM
                     LWSVVQVVLIPIALGIVLQIINRKIAEKASTALPIISVVAISLILAIVVGGSKHQILT
                     TGLLIFLVVILHNVLGYTIGYWLARLLKLDRQDQKAVSIEVGMQNSGLAVSLAALHFN
                     PIAAVPGAVFSFIHNITGPILAKYWSKKL"
     gene            23109..23744
                     /locus_tag="POB55_000561"
     CDS             23109..23744
                     /locus_tag="POB55_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDI6499547.1"
                     /translation="MYRAVIFDFDGTIIDTEQHLFNVINKHLEMHNADPISIDFYRSS
                     IGGAATDLHDHLIKAIGSENKDKLYEEHHLTSTTLPMIDTIKSLMAFLKQRHIPMAIA
                     TSSVKAEIMPTFKALGLDDYIEVVVGREDVEQVKPDPELYLSAVQQLNYMPTQCLAIE
                     DSVNGATAAIAAGLDVIVNTNKMTSAQDFSNVDYVAKDIDYDQIVARFFTK"
     gene            23755..23946
                     /locus_tag="POB55_000562"
     CDS             23755..23946
                     /locus_tag="POB55_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499548.1"
                     /translation="MMGYIILFFLAGPVILGVGNLVIGPIFNKQTPFRVQVRSFVVGS
                     MIYLILATIGYFLLLQGKL"
     gene            complement(24042..24227)
                     /locus_tag="POB55_000563"
     CDS             complement(24042..24227)
                     /locus_tag="POB55_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499549.1"
                     /translation="MTTALIGVIVLILLIISFVPNYRAMKSAKEQGSNATRATIIVGI
                     DIILIVLIIVTLLLKYM"
     gene            complement(24224..24580)
                     /locus_tag="POB55_000564"
     CDS             complement(24224..24580)
                     /locus_tag="POB55_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499550.1"
                     /translation="MLKQILLRATKISVLFAVAFFIINYIGMEKPDILYLVGRTIIAT
                     LAFILICLTVFTIINSPERKIKLGTTLPIALIIGIIFGAIFLTVQIGVITGLIIGVIA
                     TFIWELIEKNKGGRSS"
     gene            24860..26239
                     /locus_tag="POB55_000565"
     CDS             24860..26239
                     /locus_tag="POB55_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDI6499551.1"
                     /translation="MENNELQRGLSARQIQMIALGGTIGVGLFMGATSTIKWTGPSVI
                     LAYLIAGIFLFLIMRAMGEMIYLNPTTGSFATFASDYIHPAAGYMTAWSNIFQWIVVG
                     MSEVIAVGEYMKFWFPELPTWIPGVIAILLLMAANLFSVKAFGEFEFWFALIKVVTII
                     LMIIAGFGLIFFGFGNGGHAVGISNLWTNGGFMPNGIVGFFFALSIVIGSYQGVELIG
                     ITAGETKDPQKNIVKAVNGVIWRILIFYLGAIFVIVSVYPWNQLGDIGSPFVATFAKI
                     GITFAAGLINFVVLTAAMSGCNSGIFSASRMIYTLAHKGEMPKIFTKIMRNGVPLYTV
                     IAVSLGILIGALLNVILPLYIDGAKSIFVYVYSASILPGMIPWFMILFSHLRFRKLHP
                     EELEGHPFKMPGGAVTNYLTILFLILVLVGMVFNVETRISVLIGVIFLTIVTIYYFIR
                     YNKNNVKAK"
     gene            complement(26626..27498)
                     /locus_tag="POB55_000566"
     CDS             complement(26626..27498)
                     /locus_tag="POB55_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDI6499552.1"
                     /translation="MSNVLTEIDSQYPYMTKNEKKIAKFILNSPQKVIKMRSQDLASL
                     LDISTSSVIRFSKKITDGGFHDLKINISKYVPKASSIYNVELMNNESTESLRTKLHTR
                     TTRALNHANNELNDKTIDQICHCLKRSETIFIYGFGASFVVATDLYQKLSRIGLNIQL
                     VQETHIFATLLATHNSNDSVILITNNGTQSEMQSMVKVIDDYHIPIITITSTRDNPVA
                     QASNIVLTYGKTDENEMHMGATTSLFAQMFTIDILYYRYVALNYHASLDFITQSKMAL
                     DNYRKHLSNINFKH"
BASE COUNT         9245 a         3818 c         5396 g         9183 t
ORIGIN      
        1 gtgattttac attaatgccg atgggtctta atattttgtt agtacttctt tattttgttt
       61 atttaggcct aacaattgtt atgatagtat tcctatgctt tgacagtaag ccggcaaata
      121 aatatggcga aagcccaaaa tacccgtcta caattaaaaa tcaaccagtg acatctgaaa
      181 caccgaaaac accggaagaa acagataatc aattgtaatg atataaatat ctcggcacaa
      241 tgatgctggg atattttttt acaaataagg caataaagat atgggaaatg cgtatctagc
      301 gctaatgata cttaacatgc aaagaagata ctatttaaat atttcaattt cagaaatttt
      361 aaaaataaat tcactttaaa aatattgcat ttatagagaa tagaaatgtt atattaagat
      421 aaatacatat ttttttagga ggcaaagtat gggacttgta attgaacatg ttacgaaacg
      481 ttttggcaag atgacggcag taaatgatat ttcattagag ttagaatcag gcaaaatgtt
      541 aggttttcta ggaagaaatg gtgcaggtaa aacaacgaca tttcgtatga ttttaggctt
      601 aagtgaacca actgaaggac acattacgta taacggtaaa aaactagata aaacaatgta
      661 caatcgtatt ggttatttac cggaagaacg cggtttacat gggaaattga cagttgaaga
      721 agaattgaaa tatttggcaa ctttaaaggg aatgtcaaaa acagaaatac aacagcaaat
      781 atcgtattgg cttgagcgtt ttgatattac tgaaaaccgc aaaaaacgaa ttgatagtct
      841 atcaaaaggg aatcaacaaa aaattcagct gttagcaagt atgttacata aaccagaatt
      901 actaatatta gatgagcctt tcagtggtct agacccagtt aatgttgagt tattaaaaga
      961 agcagttaaa gacttaaatg attggggcag tacaatcgtt tatagttctc accgtatgga
     1021 gcatgttgaa gaattatgtg atgatgtttg tattttagat aaaggtcaac ttgttgtttc
     1081 tggtgatatc aatcatgtac gtgcatctaa tggtaacaag aaagttgtca ttgaatcaga
     1141 aacaacacta ccagatttaa caaacattag aggcatcatt catagtgaaa acatgaagca
     1201 aggtcttcag ttgacgattg agaatgaaga cgtagccaaa gatatttatc aagttgtggc
     1261 acatcaaggt tatgttaaac gattccaagt tgtagaacca tcattacaag atatattcat
     1321 tgagaaggtt ggtggcaagg atgcataagt tttgggcgac atttactttg acatatatca
     1381 aaaaaattaa agctaaatca tttgtaattt ttatgattat tattgctgcc ttaatgattg
     1441 gcctatctaa cattgataag attattaaca tgtttgatga tggtccagat aaaattggtg
     1501 tagcagcacc gaatgaacaa atctataagg ttttcaagca acaggcgaat acatttcata
     1561 gtgatgctaa gtttactaaa gtttctatag aagatgctga aaaagaagta aaaaaacata
     1621 aacttgataa agcttatatt atcaaggtga atcaaaatcg cacgttacaa gggacaatca
     1681 ttagtgagaa gcgagtaagt catgaagatt cacaaaaagt acaagcactg cttacagcta
     1741 ttcaaacaaa tatggtagcc ggggaattaa atatcaacaa agaagactta caaaaattac
     1801 aagcacaaag taaagttgat aataaggtga tttctaatga tgaagtcgat aaagtatcag
     1861 aaggacaaaa gattttcaac tatgctttag cttatggcat tatctttcta atgttcttta
     1921 ttgttttaaa ttatgcatct caaatagcta tggaaattgc tagtgagaaa acatcgcgtg
     1981 tcattgaaat gattattaca agtatttcac caatccaaca tatttttgct aaaattttag
     2041 gcgttatagc agttgccgtg actcagatta ttttaattgt attgatggca gttatatgca
     2101 tttttgcctt tgacttaaaa gacttgctac aaggttttaa cgtggaaatg aatcaattgt
     2161 cttggcaaat tattattgtc ggaattataa gtagtattgt tggaattatg gcgtatgtat
     2221 tacttgcagc catcctcgga tcattaacat ctagaataga agatttaaat caatctttga
     2281 tgccactgac attacttggt atgattgcat tttatatcgc gatattcacg ataaataatc
     2341 ctgatgggcg acttgcttta atcacaagtt atattccgtt tttagcgcca tttcaattag
     2401 ttgtacgcgc tcaaacttca gggcttcaaa tatatgaggt catcttaagt tctttaatct
     2461 caattgtaat ggtagctgtc ttgatatgga ttgcaattaa aacatataaa gattcagtac
     2521 ttacatttga acgtggctta tttaattcaa tgaaacgtgt atttaaaaaa taaaattgat
     2581 gagagtgaca cacatttatc atataaaatg atgatgaaag cgtgttactc ttattttttt
     2641 atatttagct atagaataat gtctaaaaaa gtgtacaata tgaatgtgaa aacgaacttt
     2701 ttaaaatggg aatgtgaagt aagaagaaat gtaaacaaat gatatgtagc cgatagatta
     2761 ttggtagcaa atgtgtgatg gagtgatttc atgttatatc cgatttttat atttatatta
     2821 gctggcttat gtgaaatagg tgggggatac ctgatttggc tgtggcttag ggaaggacag
     2881 tcttcacttg ttgggctaat aggcggtgcg atactcatgt tatatggtgt cattgcgaca
     2941 tttcaatcat ttccatcatt cggaagagta tatgcagcat atggtggcgt atttatcatc
     3001 atgagtttga tttttgcgat ggtcgtagat aaacaaatgc cagataaata tgatgttatc
     3061 ggtgcaatca tttgtattgt aggtgtttta gtaatgttgc tacccagcag agcttgaaca
     3121 cttgtggtta gaagggagaa gtaaaaatga tagttgaagt tgagcaccaa agacaaagtg
     3181 tagatttaat caaaattgat aatgatgaaa ctaaaattat ttttacgaat tatggagcaa
     3241 gagtagtgtc ttggaaatat catgataata atattgttct tggaaatgtt gttgaagcgg
     3301 atgaatttta tcatagtaat ccttttaaat ttggtgcttc tataggaaga tatagcggta
     3361 gaattgataa tgctaaattt aagttgaaag gtaaagaata tcagttagaa aaaaacaacg
     3421 gcgaacatca tttacatggt ggatgtcatg gtttggacaa taagttattt gattatgaaa
     3481 ttagaaatga aattgctcaa attaaagtga tatttaagac ggtgttgaaa tcagctgatg
     3541 atcatttccc gggtgatatc gatgtgacga ttacacatat ttatgatgcg gatcaccaat
     3601 ggtcgattga atacgaagca gttgcatcag aagacacatt gtttagtcct acgaatcatg
     3661 tgtacttcaa tttgaatcga gataataatg tcgttgataa tcatcgcatt tcaagtaatc
     3721 aattagatat gtatgtatta gatgagcgaa atattgttac aggtgatatt ctagatttac
     3781 atgaagtgtt tgaagataac aagataaaat tgagtgatat ttttacatca caacatgcgc
     3841 aattaagcca acaaatgaca cgttttggtg gtctcgatca cccatttaca gttggtgaac
     3901 ataaaatgta tgttgaaaat catgaattta tgttagaggt cgatacagat atgcctcatg
     3961 ttgtgttctt tacttttaac cagcctgatg agtgggatag tccatttaat atttataaac
     4021 cacattctgg ctttacatta gaaacacaat ttttaccaaa tgatattaat ttatatggtg
     4081 aaaaagcacc gtcaatttta aaagctggtg aagtgttcaa ttcgaaaaca agtttccgta
     4141 tcgaagaaaa agtagaaaat gattaaaagt atcggcattt atataatatt tatgttattt
     4201 ggcggaattt tgaataggtt atcaagaatc gtatcaaatg aagtgacatc attaatatac
     4261 agtttgaaat aaagaaaata tataacataa aaaaggaatc aatagcatca gtggttatag
     4321 ataagactat tatacaaacg atgtgtaagt tgattccttt ttgttttgaa aagttcattt
     4381 aacacttata atgaaaggta attatttcaa tgttctgtta tacatttttt gaagtttatc
     4441 gcgtcttgtt ggtgacagaa atggattgtt tagtgcatat cgtaacatat cttgattttg
     4501 tctatcatta taataaagtt cattaagctg ttgtacagat aaacgatgtt cgattggtgc
     4561 ttcttgtatt aagtaaagct gatcatttgg agatacatag ccttgtttta atacccggaa
     4621 atagaaacct gttttaccag atgtagacat gcgcttcact aaatcaggaa tattatattt
     4681 tgcttgaatt ttccagcatg gttctctaat ttcagaaacc tcaattaacg cttcacctaa
     4741 acgatattga ttaccaaagt aaacatcaga ttcgtctaaa taatcaaatg ttaaattctc
     4801 tccaaacatc gcatgaatag gtagtgtagg taaatcatct tgatacagtg cataattatg
     4861 cttactaaac ccacaaactg ctttatgtgg tccaccatga tctttataag cctgttcatc
     4921 accagtgaaa ccagttgaag ataaccatgt tgtttgtgaa atcttagttt tatctaaagc
     4981 agagcgcatc ggtcttttcg agctataagg caaatcttgg atttttcctg tcgaaattgc
     5041 gtgaattgat atcatatcat tctccttaac gagtattatt taaaatatat tgtactataa
     5101 aacattttta gaattgtaaa ttctgattga tatgtttaaa aaaatgttag aaataagggt
     5161 atgttaattt atacgagtca tgtaaaatat gaattgaata gattacataa atatgggagg
     5221 ctaccttcat gaaagatgtc aaagcactca agttaatgac attaaatgat gttttaagtc
     5281 aaatcaatgg tgacatgacg ctgggaattg gtacaggaag tacaatggaa ttactcttac
     5341 ctcaaatggc gcaactaatt aaagaacgtg gttacaatat tacaggtgta tgcacttcta
     5401 ataaaattgc atttttagca aaagaattag gtattaaaat atgtgaaatc aatgatgttg
     5461 atcatatcga cttagcaatt gatggtgctg atgaagtaga tccatcttta aatataatta
     5521 aaggtggcgg tggtgcgctg ttcagagaga aagttataga tgaaatggca tcgcgttttg
     5581 ttgtggttgt cgatgaaacg aaaatagttc aatacttagg tgagacgttt aagttaccag
     5641 tagaggtgga taaatttaac tggtatcaca tattacgtaa aattgaatca tacgctgata
     5701 taaaagtaga acgtcgtgta aacgaagatg tcgcatttat aactgataat ggcaattata
     5761 ttttagattg taagttacca aaaggaattg acccatataa gttccatgag tatttaattc
     5821 atctgacagg tgtgtttgag acaggatact tcttagatat ggcagatcaa gttattgtag
     5881 gcactcaaga gggtgttaaa attttagaaa aataatatat agtatagaaa agcgcttgct
     5941 agcatcatct ttttaagagg tactagcaag cgctttgtta ttatttgttt ttatctgaag
     6001 attgttcttc agttttttcg atatgttttt ctgcatcaat tacaactgat gaacgtctgt
     6061 cttctttata agtcgcaggt tcttttaatg attgctctat ttcatcttca tttgaatcgt
     6121 ttgttaaatc gtcactttgt ggtgcttgtt taactgattc tgctgattct actgactccg
     6181 aagtgatgtc atgatcttca tcatgattag attcagtttg attacctatg tgatgttgag
     6241 gttctgtttt ttcagtacct tcatgaatgc ttggttcgtc agccactgta ttttcagtat
     6301 catttttagc aacaccgacc gttgatgcag tagctacacc tgttttttca gcatctttaa
     6361 tatttgaaga agatttttca gcctcagtat gattagtttc ttcatcgtca ttgacatggt
     6421 ctaaataatt attaggctca agttcttcaa ggtttcgtct tgttgttaaa tatgcaatac
     6481 ctcggataat taagcttaaa ccaatgtatc ctactgcaac gattgctgtt gaaatggcga
     6541 tgactttgat tgaaaataga tcgccgattt cttcgctaaa caagaaaata agtccgaatg
     6601 tcattgaagc atggaatgtc gttgcaatat aaattgtacg tcctttagtc gctctaatta
     6661 attcaccaag aatcatagag aatgagaatg tataaaggaa gttataagca gcaaattctg
     6721 taccataagt tgtatttgct gagaaaactg aatacatcaa accaacaaca atacttgcaa
     6781 agaacgtgtt cattttcgtt tcaacaatat tttgtaagta tgagcggaat ccgaattcta
     6841 ctacgaacgc catcagaata tgtccaatca gaatgtgtgt aataggtact gataagcctg
     6901 ttgattgtaa taaaataaag ctatctgcaa atgtattaaa gctgtacata ccaataatta
     6961 gaataattaa aggtaaaatt aaagctagca atagacgttc aattactttc aggctgattg
     7021 agaattttaa acctgcaagt tggacctttt tatatttgaa aacgagtata caaatgattg
     7081 cagcaattaa tggtgctaga tctgtaactt caaatataaa gtgtttgaca ccaattatag
     7141 actggaaatc tctgagcata atggataacg ccattgtaat gacaaagaag acgaaaatcg
     7201 tcattgccca ttgaaaacca gaaattttat tgttcttcat tatatgtaac ctccattagg
     7261 taacaagcaa aatatctttt agtaacaatt atacatatta caagccgaga gataaattgt
     7321 ctatgtcaat attaatgaaa atgtaatagt cttgacgtat tttattaaca taaatcagct
     7381 aagtattcgt tattacatag aaaatagggg taaaagtaat gtagataagt atttttaagg
     7441 tacggattta actatgaatt gaattcaaaa tttcctatac gttatacttt aattgttaat
     7501 aagccacata aataagaggg ggaaatgctg tgtacaagca aggtgaacca aatttatgga
     7561 ctggaaggtt agatagtgaa acagacccga aaaaatttag acattttcaa acagtaacat
     7621 ttgaagattt gtctaagctg gaaaagagta gtatgccatc aggggtcggt atattaggct
     7681 atgctgttga caaaggtgtt gctttaaaca aggggcgcat tggtgcaaaa gaagggccag
     7741 atgcgattaa acaagcattt gcaggtttgc cggatttgaa tcaatgtgaa actttagtcg
     7801 attacggaaa tgtttatcat gatcatgagg aattaattga tactcaaaaa gaatttgcta
     7861 tgcttgcagc gaagtcaatt gctaatcata gacaaacatt tttattaggc ggtggacatg
     7921 atattgcgta tgcacaatat ttagctacgc gtaaagttta tccaacacag tcaatcggtg
     7981 tgattaacat tgatgcgcat tttgatactc gtgctgaaca agaatcaaca tctggtacga
     8041 gttttagaca aattttagaa gaggatgaca atactgatta tttagttctt ggtatagctc
     8101 aaggtggtaa tacgcaaagt ttatttgatt atgctaaaga gaaaaagatt gattatgtct
     8161 ttgcagatga attattaaac catgtgtcgc cacctattaa agatatgatt gagcgttttg
     8221 tgcatgaaca tgacgtcatt atgtttacga tttgtatgga tgtcattgat agtgcatttg
     8281 caccaggggt aagtgcgcct gcagtgttag gtttgtatcc acatactgtt cttgaattag
     8341 caaaacgtat tattccaagt gataaggtgt cttcagttag tattgctgag atgaatccaa
     8401 catatgacgc tgacaataga actgctaagc tcgttgctaa tttagtgcat cattttttaa
     8461 aataattaaa aatatttata tgtgacgaga tttgcattgc agggcagaat gtgagtattt
     8521 cttaatagaa gttatattaa cgttgatata tttgaaaaat aattgcagta agaagactct
     8581 taagttgtag tcatcttact gcttatttat gtttgttggt ctgaatgtaa tgatttatac
     8641 atgtaaatca taaaattggt tgtgaaattg ttttacagtt gactgagcaa ctgcataaga
     8701 ggctctaata acttaagtta aaggcaaaag gtatacagtt gactgagcaa ctgcataaga
     8761 ggttctaata acttaagtta aaggcaaaag gtatacagtt gactgagcaa ctgcataaga
     8821 ggctctaata acttaagtta aaggtgaaag gtatacagtt gactacgcaa ctgcataaga
     8881 ggctctaata acttaagtta aagagcctct aaaagtccat ataaataatc ttggcattgt
     8941 tgtacgaatg tatgtacttg ttcggagtgt ttgcgttttt tatggtaatt gatataaatt
     9001 tttcggccta agtttggacg aattttttta tattctaaat tagacgtgtg aaatgattgg
     9061 taataaaatc tcggaatgat agcgtaacct aagccgagat gaacaaatcc aactgctgat
     9121 tcgaatcgat ctgtttctac aacgacattt ggacgaatat ttcttctatt aaaatagtca
     9181 tctaagtgtt tgcgcacttg agaatttttg tttggtaata tgagtggcaa attttcaaca
     9241 tctacccaat tttgattttt aaatgtttcc ttgggtgcta ataaaatgta agattcctca
     9301 tataatggaa tagattttat atcttcgtgg gttatttttt catttgttat agctaaatga
     9361 atattaaaat tcagtaattg ctctatagat tgatgtttat catgtatttc atataaacga
     9421 tattgctgtt cagggtagtc ggaatggtgc tttcgaatta aattcgcaat ccattgattc
     9481 gtagattcaa gagtcccaat ttttatcctt ggttctgatg taacgcttaa atcatacatt
     9541 ttttccatcg tggatcgata ttgttgaact aattcgctcg cataacgata aaactgtatt
     9601 cctttttcgg taatcttgat gtcttttgtt gaacgtgtaa ataagtcata acctaaatct
     9661 gcttccattt ttttaatcgt agcagttaaa gatggctggc taatatgtaa aaattgtgca
     9721 gctttagtaa aactattata tttcacgata gccaagaagt attctaactg aataattttc
     9781 atgtgttttg ctccttttat ttatagattc aagctatgga taattagttt atttatattt
     9841 gttggctata ttttaacata ctataatgaa tattgaagat aataatgtaa gcgttttaaa
     9901 tttagtggaa ttaacttcta aaaagaacag gagcgaattt tatgagaaaa attcaagcaa
     9961 aaaaaggttt gagtattgag tgtaaaggtt gggaacaaga agcagtactt agaatgttat
    10021 acaacaattt agacccagaa gttgctgaaa gaccagaaga tttagttgtt tatggtggta
    10081 ttggaaaagc agcacgtaat tgggaagctt ttgaagcaat agagaaaacg ttacgagaat
    10141 tagaatcaga tgagacaatg ttagtacaat caggaaagcc agttgctgta tttaaaacac
    10201 atgaagaagc gccacgtgta ttaatttcaa attcagtatt agtgcctgaa tgggcaaact
    10261 gggatcattt taatgaatta gataaaaaag gtttaatcat gtatggacag atgacagccg
    10321 gtagttggat atatattggt tctcaaggta ttgtgcaagg tacttatgag acatttgccg
    10381 agctaggtaa tcaacacttt aatggagatt tagcaggcac tgttacactg acagcaggtt
    10441 taggtggtat gggtggtgcg caaccgttag caattactat gaatcatggg gtagcaattt
    10501 gcgtggatgt tgatgaaaca cgtgttgata agcgaattga tacgaaatac tgtgatgtta
    10561 aaacagctga tttagatgaa gcattaaaat tagcagaaga ggcgaaagaa cgtggagaag
    10621 gattatcaat cgggttagtt ggaaatgctg tagacatcca tcaagcgatt ctagaaaaag
    10681 gatttaaaat tgacattatt actgaccaaa caagtgcaca tgatccgcta aatggatatg
    10741 tgccacaagg atactctgta gaagaagcga aagtattgcg tgaaaaagat ccgaaaaaat
    10801 atgttgaact gtcacaagct tcaatggcaa agcatgttga attaatgctt gaattccaaa
    10861 aacgtggcgc tgtagcattt gattatggta acaatattcg tcaagtagcc ttcaataacg
    10921 gagtgaaaaa tgcttttgac ttcccaggct ttgtaccagc ttacattaga ccattattct
    10981 gtgaaggtaa agggccattc cgctttgctg cgttgagtgg tgatccaaaa gatatcgagc
    11041 gtgcggatga agaaatgcgc aaacttttcc cagaaaatga aaagttatta agatggcttg
    11101 atttagctga agaaaaaatt tcatatcaag gactaccatc acgtattgct tggttaggct
    11161 atggagaaag agcgaaaatg ggcttagctt taaatcgtct tgtacgtgac ggtgaaattt
    11221 cagcaccaat tgttattgga cgagaccatt tagatgctgg ttcagttgct agtcctaacc
    11281 gtgaaacaga aagtatgaaa gatggtagtg acgcggttgg tgattgggct gtattgaatg
    11341 cactcattaa cacagctgca ggtggttcat ggatttcatt ccatcacggt ggcggtgttg
    11401 gcatgggata ttcacttcat gcgggtatgg ttgttgtagc agatggatca gagcgtgctg
    11461 aaagaagatt ggaacgtgta ttgacgactg acccaggtat gggtgttgcc cgacatgttg
    11521 atgctggcta tgacatcgct attcaaacag ctaaagaaaa aggtattcat attccaatga
    11581 ttgataaagc aggtgataag taatgaatga tttaataatt aatcatatag cagaattaat
    11641 tttaccgaga tcaacagata aacctttgaa gggtaaggaa ttagatgaat taaatgttgt
    11701 gaaaaatggt acagttgtca ttaaagatgg caaaattgta tatgctggaa cacatacaga
    11761 tgactacgat gcgactgaga cgattgatgc tagtgggaag gtagtgtccc cagcattagt
    11821 agatgcacat acccatttaa catttggtgg atctcgagaa catgagatgt cattaaaacg
    11881 tcaaggtaag tcttaccttg aaatattaga aatgggtggt ggcatattat ctaccgttaa
    11941 tgctactaga gaaacatccg aagatgactt gtttaaaaaa gcagagcatg atttgcttac
    12001 tatgattaaa cacggcgtgc ttgcagttga aagtaagagt ggttatggat tagatagaga
    12061 aaatgaactg aagcaattga aagtgtctaa tcgtttagct gagaaatatg atttagacat
    12121 gaaacatact ttcctagggc ctcatgctgt acctaaagag gcaagttcaa atgaggcatt
    12181 tttagaagaa atgattgcgt tacttccgga agtaaaacaa tatgcagact ttgcggatat
    12241 tttctgtgaa acaggtgtat ttacaataga acaatcgcaa cattatatgc aaaaagccaa
    12301 agaagcaggt tttaaagtga aaatacatgc ggatgaaatt gatccgttag gcggactgga
    12361 attagcaatt gatgaacaag caatatcagc agaccactta gtagcttcta gtgataaagg
    12421 aaaagaaaag ttgcgaaata gtgatactgt agctgtcctc ttacctgcaa cgacgttcta
    12481 tttaggtaaa gaagattatg cagatgcaag aggcatgcta gacaataacg gtgcgattgc
    12541 attagcaact gattataacc ctggtagtag tgtcacaaac aacttacaac ttgttatggc
    12601 gattgcagcc ttgaaattaa agctatcacc taatgaagtt tggaatgctg ttacggtcaa
    12661 tgctgctaaa gcaatagaca ttaacgcggg tacaattaac acaggtgata aggctaattt
    12721 agtaatttgg gatgcaccaa atcatgaata tattccatat cattttggta tcaatcacgc
    12781 agaaaaagtt attaaagacg gtaaggtcat cgtagataac acattatctt ttaaagcata
    12841 aaataattag aaagctctaa gcattggttt acactaatgt ttagagcttt tgtggtttgt
    12901 aaaaacaagt ttatccgaga acacgttttt gtatagattc ttaattataa aagttagaat
    12961 agtttgacaa cagtgttacg atattattaa acaactgtct atcattagcg aaagtaatta
    13021 acaagagtac attgttgtga aatcatcgtt taatagtcaa agatgatcat cgtagggtag
    13081 acaacatatc attgacttaa ggaaggggaa tgaccgatgg gattaaaaga tcaagctgtt
    13141 acctggagaa ggtattttca tcaatttcca gaactatccg ataaagaatt taagactaca
    13201 caaaaaatta aagatatttt aacagaacat catattagaa tattagactt accacttgcg
    13261 actgggcttg ttgctgaagt gggacaaggt ctgagttgta tagctgttcg ggctgacatt
    13321 gatgctttac cgattcaaga gcttgttgaa caggatttta aatctgaaaa tgaaggtgtt
    13381 atgcatgcgt gcggtcatga tattcacatg gctagtattt tggctacggc tgtaaaatta
    13441 aaagaaattg agggcactct tactgggcgt gttaaattca tttttcaacc agcagaagaa
    13501 ctaggacatg gcgcttttaa aattattgaa acacatgcat taaaagatgt acaagcagta
    13561 ttgggttttc ataatgaccc atcgcgttcg gtaggtacat ttgcaatcaa aacaggggca
    13621 attacatcag cagtagatcg ttttgagttt catattaaag gcgtgggtgg tcatgctgca
    13681 aagccagaac aatgcaacga tccagttatt gtgttggcgc aattgattaa tagtattcaa
    13741 tccatagtta gtagaaatct atctgcgttt gatgaagcgg tagtaacaat tggacaaata
    13801 tcatgtggta atacttggaa tgtcattgct gatcatgcgt atgtacaagg cacagtgcgt
    13861 agttttgatc ctgtggtacg aaagcttgta gaaacacgat tacaagatat tgcggacgga
    13921 ttggctcaag catataatat gaaaattaat ttaaattata cacatttacc tggcgctgtg
    13981 atgaatgatg aggcattgac gcataaagca attgcagtag cccaacatgt tggatataag
    14041 gcggaaatga tggaacagcc gcttacgatt ggagaagatt tttcaggtta tagtcaacat
    14101 tttccaagtg tatttgcgtt aattggttct catagtgaat atgatttaca tcatcctcaa
    14161 tataaaccgg atgaaaggat tcttgaaaaa gtacctgagt attttgttga atttgtaaaa
    14221 agactattgc atgagtaatt cgagcttgtt tttataaaga agaaagtaat ttttagtgta
    14281 atcatgatta agttcctagg aagattgggt aaataataac cattaagcga ataaggagat
    14341 gttaaaaaat ggcagctcaa gatcctagaa caaaatttaa aacaacggat tatgaaaaac
    14401 aagaacaaga ggtaccgggt ttacaatctg aaatgacgcc agcacctgat tgtggcgaaa
    14461 cgtcttacca aggtcatcaa cgcttacaag gctataaaat attagtcaca ggtggtgact
    14521 cagcaatagg gcgtgcagca gcaattgcgt atgctaaaga aggtgcagat gtagcgatta
    14581 actatcttcc gagtgaagaa caagatgcac aagaagtacg ccaagtgatt gaagaaagtg
    14641 gtcaaaaggc agtgttaatt cctggtgata taagagatga gcagttcaac tatgaccttg
    14701 ttgaacaagc atatcaacaa ttaggtggct tagataatgt tactttagtt gctggacatc
    14761 aacagtatca tgatgatatt catggattta caactgaggc atttacagaa acatttgaaa
    14821 cgaatgtcta tccgctattt tggacagttc aaaaagcatt ggaatattta aaaccgggtg
    14881 cgtcaattac aacgacatca tctgtacaag gttatcatcc tagtcctatt cttcatgatt
    14941 atgccgcttc aaaagcagca attatttcat taactaaaag tttttcagaa gaattaggac
    15001 caaaaggaat tagagtgaac tgtgtagcgc ctggtccatt ctggtcacca ttacaaattt
    15061 ctggtggaca acctcaaagt aaaatcccaa catttggtca aaaaacacct ttaggtcgtg
    15121 caggtcaacc tgttgagtta tgtggaactt atgtgctatt agcgtcagaa gaatcaagtt
    15181 atacaactgg acaagtgttc ggtgttagcg gtggagtgca aatagattag gtttgtatga
    15241 agcttttaat tgaacaacat aatattaatt tagttacttc aatccattaa attaaaatga
    15301 attggtgaag tgtattttaa atagtcggta aaggtgtgta tctttatcgg cttattttaa
    15361 tactctgtca taaggagcgt ttgctcaggg tgtggattat aaggtttaag cagaataaac
    15421 tttcttaatg aggatgtcag gacaacaacg aaaaaattaa aaaatgttgc gggattgggt
    15481 ttagggacgg ttagtggaag aaagttaaat tcattaacaa gtgaaatacc taaggctaga
    15541 tattaaacgt cagaacggac aaagaataat aggatatgtt ttcaattaaa tagcgaagta
    15601 tttatatgtt aagatgatgt aaattacata gaaacgctac ccgtagaagt actgatcata
    15661 gaaacgctga taaacgtaaa tttgcaaact cgatattaaa caaaggggtt gttattatgg
    15721 cattaatagt atctagagct atattatttt tgattttagc attagctttt ggaatagcga
    15781 ctgtttttcc tgaacaaaaa ttaccattat atatagtatt cacattattt gtagcagttg
    15841 cgatcgtttc gaatgtatgg tttacacatc aaaacgttaa aaagcatatc attaagttta
    15901 acaaatacgt cgcatacttt ttatcctttg tctcagggtt actttacatt ggatttgtaa
    15961 tttatacatt aactataggg agctatgatg ttttaccaat tattatattt gcaataagta
    16021 tcgtattggt tcaaatatta gaaaatatac tgggaatcag tataaaaaat acgcgtctat
    16081 caaacaaaca gttgaaagtc atctccaagc aacgtgttca gcaatttaaa gtcatatcat
    16141 taatattagt aagtgtgtct atcgtgttca tttttattgc attaatgctt aaatggtggc
    16201 ttttacttgt tattagcatt attgttttga ttattattac agtagcaatg attgtttatg
    16261 aaaaatatta tacgactcct gattaattta tgattgacgt actaaggatt atcttgtaac
    16321 gtaaacgtaa ataagcatca cttatctaga gaggtggagg gactggccct atgaagcctc
    16381 ggcaacatct cgaatgtgcc aattccagta accgtaatgg tttgaagata agcaggtaaa
    16441 gcacatgaaa acctctttct tcatcgtttg tgagaaagag gtatttttaa ttggaaagca
    16501 ggtaaaaagg atggaagtac ataaaaagag caatgcttgg gcattattcc ccttgttatt
    16561 atttgtggcg ttgtttttag gcgtaggtat tatcacaggt gattttactt caatgccatt
    16621 aaatgttgca attacgataa cggtaattgt ggcgttatta atgaatcgaa aagaatcatt
    16681 tgctaaaaaa gttgaggtct tcacgaaagg tgcaggccat tcaaacatta ttttaatgat
    16741 gttgattttt attttagcag gtgcattttc aaatacagct gaaaaaatgg gtggcgtaaa
    16801 gtcgacagtt aatttaggac tatcattaat cccagaaaat ttaattattg taggattgtt
    16861 tgtgatttgt atgtttgttt ctatatctat ggggacgtct gtagggactg ttgctgcaat
    16921 agcacctgtt ggttacggat ttgcacaagc gacagatgta ccaactgcgc tagcaatggc
    16981 tacagttgtt ggaggtgcaa tgttcggcga taatctatca atgatttcag atacaacaat
    17041 tgctgcggtt agaacacaac atacgaaaat gaaagataaa ttcagagtaa actttaaaat
    17101 tgtattgcca ggtgctattt taacgattat tattctgtat ttcttaacga atggcatttc
    17161 tttaaatcat gctaaaagct atgattataa tctaattaaa gttgtaccgt atattttagt
    17221 attggtatta gcattattag gtgtaaatgt cattatcgta ttgattggcg gaacattatt
    17281 agccggaatc attggtctca tagatggttc attcggttgg atggggctac tagatgcagt
    17341 gtccaaaggt attataagca tggaagatat tgctatgatt gcgttgctaa ttggtggttt
    17401 agtcggtatt attcaacaca atggtggtat tgaatggttg ttgcaatttg ttcgctctaa
    17461 agtgaaatca aagcgtggcg cagaattagg aattgcgagt ttggtaagtg tcgcagatat
    17521 tgccacggca aacaacacca tctctattat tatgtcaggt cctttagcta aaaatattgc
    17581 agatgaatat gatgtagatt caaggaaatc agcaagtatt cttgatatat ttggaggctg
    17641 cttccaaggt ttattaccat atagcccgca agtcatttca gcggctggtg tagctggtat
    17701 ttcaccgtta atgttattcc catacagcat ttatcctata ttattaggtg tatgcggact
    17761 tattgcgatt ttatttaatt ttccaaagct aaaaaagaac actagtcaag aaatgaaaca
    17821 ttaatgctta acaatatatc gatgtagcca agtaaggtgg cttgttatat taacagatca
    17881 tgttgtactc accgtataac gctaatcttt taaaaatgat gatcatctat gtttggaact
    17941 gagcaaaaat acgttaaaat aaaagtaaga gcgaaagtgg gtggacaaca gtggcaaaat
    18001 ttaatgtaga gaatgaacat gtcgaagttg aagttgaaaa gctttataaa ttttctccag
    18061 agttagtata cgaagcttgg acaaagaaag atttgttgaa acaatggttt atgacatcgg
    18121 ccagaacgaa taaagagatt gaagcggacg ttaaagaagg cggtaaatat cgtattgtag
    18181 atcaacaacg taatggcaaa gtaaatgtga ttgaaggcat ttatgaatct ttagttatgg
    18241 atgaatacgt taagatgact atcggtatgc ctggactaag tgaaacacaa gatgtcattg
    18301 aggtagaatt ttttgaacgt gaaacaggtg gcacccaaat gctattttat tatcgttcat
    18361 tagttgaaaa agaaagacga tttactaatt tagaatacaa gcaaaagaaa aaagaatatc
    18421 atgatgccat ggtgcatggt ttcgaattga tgtttgataa gatgtaccac gttatcgaaa
    18481 catcaacaca acaataaaat gataaaagga agttatgata ggctcaaggc cattaaatca
    18541 taacttcctt ttttctaatt tgaaatatca ttatgtagta aatactcatt atattttctt
    18601 acaagtgttt caaagaatat atgataactg atgaagctag aatagtctgt accatcagtt
    18661 aagtagaatg tgttactgga tacatagacg ttgtaatttg ctttttgagc aagggtatta
    18721 tctttcattg tagtaacaga aataaagtat ttttgtctaa gttgaagcaa gtttgttact
    18781 tctaataatt catgtgtttc acctgataat gaaataataa agaataaatc attaggttca
    18841 ctcttgttca aaatcatttt aagttcatgt tcatcatgaa gtacaatgac atgattatgt
    18901 aacgttaaca atatacgctg tgcctctgaa gcaacgttta actgtgcacg ccctgtacca
    18961 tacaaatata ttgttgaagc tgtatgcatt ttttcagtaa gaagctggta atcaacgcgt
    19021 tctaaataac tgaatgtact atcgatttct tgtttgaaac tatcgataga gttagaaggt
    19081 aattggtgtg tcttattact ttcaaattta aggtacgatt taaaatcact ataaccgtca
    19141 aaacctaatt tgcgtgtaaa tctgtgaatg gttgcattcg aggcatgtgt gaattgcgct
    19201 aaatcttgta ttttcatatt tttacattca tctatatgtg tattgataaa gtgagcgata
    19261 tgtaaatcat tatcattaag tttatcaaag tttcgattaa tgtgttcatc taaaaacata
    19321 ttcaacctcc ttgaaaataa atgtgtgata gatcgttatt acattaaaat cttgtatgtc
    19381 attatatata tttgtgtatt gatataggtt tgattatgcg tttaaaacga atgtattaca
    19441 aatacaacga tgtcatatat aaggtcgtga aaaaattttc actatatgaa aagataatct
    19501 gattatacaa aatactttca aaatacgtca atgtcgttgt aggtgtgtaa gcgtttacta
    19561 tatattatat ttaaatagta agacatctat ttataaatga atctaattag atgtattgtt
    19621 aggaaagatg atatggaggg aatagaagat gaatgcgata aagcgtttcg gtagtgcaat
    19681 gattgtaccg gtattaatgt tcgctttctt tgggatagtg cttggctttg cgacattatt
    19741 taaaaatcct acaatcatgg ggagtttagc agatcaacat acgttttggt ttaaattttg
    19801 gtcagtaata gaatctggtg gctgggttat ttttacgcat atggaagttg tatttgttgt
    19861 aggtttacca ctttctttag ctaaaaaagc accaggtcat gcagcacttg cagcattgat
    19921 gggttactta atgtttaata catttataaa tgccatttta acgcaatggc cacatacgtt
    19981 tggcgctaat ttagaaaaag gcgttgaaaa tgtacctggt ttaaaatcaa ttgcaggtat
    20041 tgccacattg gatacgaata ttctaggtgg tattattatt tcagctatta ttacttggat
    20101 tcacaataga tactatagta aacgattacc agaaatggtt ggtgtattcc aaggtttaac
    20161 atttgtagtt acgatttcat tttttgtgat gttaccatta gcagcaatta cttgtgttat
    20221 ttggccaact gttcagcacg gtattggttc aatgcaacac tttatcatcg cttcaggcta
    20281 tatcggtgtt tggctgtacc acttcttaga acgtgtatta ataccaacag gattacatca
    20341 ctttatttat gcaccaattg aagttggacc agtagtagtc aatcacggct tgaaagctga
    20401 atggttacaa catttaaatg agtttgctaa gagtactaag ccattaaaag agcaattccc
    20461 ttatggcttt atgcttcaag gaaatgggaa agtatttgga tgtttaggta ttgctttagc
    20521 aatgtatgct acgacaccta aagaaaatcg taaaaaagta gcagcattac ttattcctgc
    20581 aacattaaca gcagtcgttg taggtattac tgagccatta gaatttacat tcttattcat
    20641 cgcaccatac ttatttgttt tacacgcagt tttagcagca tcaatggata ctttaatgta
    20701 cgcatttggt gttgtaggta acatgggtgg tggcttgtta gactttattt caacaaactg
    20761 gttgccatta ggtaaagaac attggggtac ttatgttgca caagttatta tcggtctgat
    20821 atttgtagcg atttacttct tcttattcag attcttaatt ttgaaatttg atattccatt
    20881 acctggacgt aagaaaacag aagaggaagt taaattattt tctaagcaag attacaaaaa
    20941 taaaaaaggt gatagcgtag attctaaacg tgcttcaaca ggtaatgaat acgaagacaa
    21001 agctgcgtat tacctcgacg gtttaggcgg caaagaaaat atcaaagatg ttactaactg
    21061 tacaacaaga ttacgcttaa ctgtttatga tgaaagtaaa gttgcggata cggaatattt
    21121 cacgcatcaa caaatggcac acggattagt aaagagtggt aagagtatcc aagttgtagt
    21181 aggtatgaca gtaccacaag ttcgtgaagc atttgaacaa atggttgagg atcaatcgtc
    21241 cgaagataaa taatacaaaa aaagacacat cactatacgc aatcattgta aatgatcatc
    21301 gtatagatgt gtcttttgtt atgatatacg agctttgctc atagatgacg atctataatt
    21361 ggcagataga atgtcgatat ttctagttgc aatcgtatgt aacaatttag caaatatttc
    21421 tggcgtacat ataatatgtg catgagactt attcaacgta taagtgatat cagtgtgatt
    21481 ttggaacata ttcataatat cgatgacttt gtctttaatg aaagcatctt gcaagccatc
    21541 aatattgata tcgaaacgct ttgcagattg atataaatta tcagaatcaa gtaattcatt
    21601 tattttcttt ggtgaaagta cttggaaaat actatactga tgaaaactta aaaatgaaat
    21661 gaagtatgga agttgttgct ttggtaagct aatttctaat acatactgat taaactgttt
    21721 gtttattgag aattgcaacg catcacaaaa ttgtacactt ttgcataact tacttaattt
    21781 ttttctagta gtacggtcat ttaaaccggc gatgttgatg atgtatgttt cgaatttatc
    21841 aattaacatg tgaggacctc ccgaggaata catggcatta aatacacgtt taatatttat
    21901 aaaggtgact taattttgtt caagttgatt ttaccacgct ttttttcttt attcactaag
    21961 acttttgaat gaagtttaaa ataatttttt atcagtgata aaatatttgc aataagaaga
    22021 gaatgactaa ataatcttaa ttttcagaaa agtaattgta accttactgg tcttatggta
    22081 atatttttca atattatcga cgaggatgtg ttaacaatgt ttcaaaaatt gagtatattt
    22141 gccacgaaga gttttttggt atggatgtta gtagcagctg ttattggatt tatttcccca
    22201 caacatgttg cgacattagg taagtgggta ccttatttac ttggtattgt tatgttaggt
    22261 atgggattaa caattacacc taatgatttc aaaatggtct ttaaagcacc tagagcagta
    22321 attattggtg tctgtctaca attcagtatt atgcccacat tagcatttat aattgcaaag
    22381 tcttttcatt taccacctga tattgctgtt ggcgtaatat tagttggatg ttgtccgggt
    22441 gggacatcaa gtaatgtaat gagttattta gccaaagcta acgtagcact ttctgtttct
    22501 attacgacgg tctctacgtt gctagcgcca ttcgttacac ctgcgttaat atatctattt
    22561 gcaaatgaat ggttggaagt atctttcttg agtatgttgt ggtcagttgt tcaagttgta
    22621 ttaattccaa ttgctttagg tattgttttg caaattatta atcgtaaaat tgctgaaaaa
    22681 gcttctacag ctttgccaat tatatcagtt gttgctattt cattaatttt agcaatagtt
    22741 gtaggtggca gtaagcacca aatcttaact acaggattat taatattttt agtagttatt
    22801 ttacataacg tattaggata tacgattgga tattggttag ctcgtctttt aaaattagat
    22861 cgacaagatc aaaaagcagt cagtattgaa gttggaatgc agaactctgg tttagctgtg
    22921 tcattagcag cattgcattt taatccaatt gcagcagtac caggcgcagt gtttagtttc
    22981 attcataata taacagggcc tattttagca aagtattggt caaaaaagtt ataattgcac
    23041 taatagaatg aagtggtcat cggactgtgt taagctttga taaagagaaa aaatagagga
    23101 gtaaatatat gtatagagca gttatatttg atttcgatgg aacaataata gatacggaac
    23161 aacatttatt taatgttatt aataaacatt tagagatgca taatgccgat cctataagca
    23221 ttgattttta tcgttcttct attggaggag cagctacaga tttgcatgac catttaatta
    23281 aagcgattgg ttcggaaaat aaagataaac tttatgaaga acatcatctt actagtacaa
    23341 cattaccgat gattgatacg attaaatcat tgatggcatt tttaaagcaa cgtcacattc
    23401 ctatggcgat tgccacaagt agtgtgaaag cggaaataat gcctaccttt aaagcattag
    23461 gtctagatga ttatatagag gtagttgttg gtagagaaga tgttgaacaa gttaaacctg
    23521 atcctgaatt atatttatct gcagtacaac aattaaatta tatgccgaca caatgtttgg
    23581 ctattgaaga ttctgtaaat ggtgcaacag ccgcgattgc agctggatta gatgttattg
    23641 ttaatacgaa taaaatgaca agcgcacagg acttttctaa tgtagattat gtagcaaaag
    23701 atattgatta cgatcaaatt gtagcgcgtt tctttacgaa ataggaggcg tatcatgatg
    23761 ggttacatta tattgttttt tctagctggt ccagtaattt taggcgttgg aaatttggtg
    23821 attggtccta tatttaacaa acagacacca tttcgcgtgc aagtaagatc ttttgttgtt
    23881 ggttcaatga tttacttaat actcgcaaca attggctatt ttttactatt acaaggtaaa
    23941 ctttaacgag aaaaccacct tacctcatta aatggacgac catatgtatg tgaaatggta
    24001 gaacgttcat gtttatgtat gagatagggt ggtttaaata gttacatata ttttaataat
    24061 aacgtcacga tgataagtac aattaagata atatctatgc ctactataat tgtagctctt
    24121 gttgcattac ttccttgttc ttttgctgat ttcatagcac ggtagtttgg cacaaagcta
    24181 ataattagta agattaatac aattacacca attaatgctg ttgtcatgat gaacgacctc
    24241 ctttattttt ttcaatcaat tcccaaataa acgtagcaat cacaccgatg attaacccag
    24301 tgatgacacc gatttgtacc gttaaaaata tggcgccgaa aatgatacca ataattaatg
    24361 caatcggtaa agtcgtaccg agtttaatct tgcgctctgg agaattaata atagtaaata
    24421 ctgtaagaca aatgagtatg aaagcaagtg ttgcaataat agttcttcca actaaatata
    24481 ggatgtcagg tttttccata ccgatataat ttatgatgaa aaatgctaca gcaaagagta
    24541 ccgatatttt tgtagcacgt agcagtattt gttttaacat tgatatactc ctttttaata
    24601 ttattaaaat tatatcataa ttaccaagag tagctgaagt tgtatgtgac tcaacggttc
    24661 acggacaact tttttaattt tttagaaaaa tcacaaaata attgtttgca aagttgcaaa
    24721 agcctgctat agtagttctg taaacgattg catggtatgc aaatattaat gtaccaaaat
    24781 cgataattta tagtataatt acggcaataa gtttttttat ggatttattt agtatcaatc
    24841 agagatgggg taagaagtta tggagaacaa tgaactacaa aggggattga gtgcccgtca
    24901 aattcaaatg attgcacttg gtggtacgat tggcgtgggg cttttcatgg gtgcgacaag
    24961 tacaattaaa tggacaggcc catcagttat ccttgcatat ttaattgcgg gtatcttttt
    25021 atttttaatc atgagagcaa tgggggaaat gatttattta aaccctacaa caggatcatt
    25081 tgcaacattt gcaagtgatt atatacatcc tgcagcaggt tatatgacag catggagtaa
    25141 tatattccaa tggattgtag ttggtatgag tgaggtcatc gcagtaggag aatatatgaa
    25201 gttttggttc ccggaattgc caacttggat tcctggtgtt attgctattt tattattaat
    25261 ggcagcgaat ttattctcgg taaaagcgtt tggagaattt gaattttggt ttgctttaat
    25321 taaagttgta acaattattt taatgattat tgctggtttt ggtcttattt tctttggttt
    25381 tggaaatggt ggccatgcgg taggtatttc taatctatgg acaaatggcg gatttatgcc
    25441 aaatggtatt gttggtttct tctttgcatt atcaattgta attggttcat accaaggtgt
    25501 ggaactgata ggtattacgg caggtgaaac taaagatcct cagaaaaata tcgttaaagc
    25561 agtgaatggt gttatctgga gaattttaat tttctattta ggtgctattt ttgttattgt
    25621 ttcagtgtat ccttggaatc aattaggaga cattggaagt ccgtttgttg caacatttgc
    25681 taaaatcggt attacatttg cagctggatt aattaacttt gttgtattaa ctgcagcaat
    25741 gtcaggatgt aactcaggta ttttcagtgc gagtcgtatg atttatacac ttgctcataa
    25801 aggtgaaatg ccgaaaatat ttactaaaat aatgagaaat ggtgtaccat tgtacactgt
    25861 tatagcagta tctcttggta ttttaattgg tgctttatta aacgtaattt tacctttata
    25921 tatcgatgga gcgaagagta ttttcgtata cgtttatagt gcgtctattt taccaggtat
    25981 gattccttgg tttatgattt tattcagtca cttacgtttt agaaaattac accctgaaga
    26041 attggaaggt cacccattca aaatgcctgg tggcgcagta actaactatt taacaatctt
    26101 attcttaata ttagtattgg ttggtatggt atttaatgtt gaaactagaa tatcagtgct
    26161 aattggtgtc atcttcttaa cgattgtaac gatttattac tttattagat ataataaaaa
    26221 taatgtaaaa gcaaaatagt atgattataa tgtgcgctta ttttatgaca aatttggccc
    26281 tagcttatta aagttaaata gccaaagaat gttatgagta ggcgcatttt tttataaata
    26341 tagcttaaat cgaagttgta gtgtgaaaaa cttataagtg gcaatattat cgttagtttt
    26401 tactgtgggc tttaaaataa aaaatagcat tattaaaata acaaacgcaa ttgatatatg
    26461 gaatgaatcc gttaatcaac tgcgtttgtt tttaatataa gaaattgaat ggccttatca
    26521 aatcattggt catatgcgta tgttatacaa gataaaagac atgtaagctt tgaaaatgat
    26581 gcataaatta ttatatgaca tgattaaaga atttatataa gactgttaat gtttaaagtt
    26641 aatatttgat aaatgtttac gatagttatc taaagccatt ttagattgtg ttatgaaatc
    26701 taatgatgcg tgataattta atgcaacata acgataatat aaaatatcaa tagtgaacat
    26761 ttgagcaaat aatgaagttg ttgctcccat atgcatctca ttttcatcag ttttcccata
    26821 agttaaaaca atatttgatg cctgtgctac gggattatcc cttgtactag taattgtaat
    26881 tataggtata tggtagtcat caataacttt aaccattgat tgcatttcac tttgcgtacc
    26941 attgttagta ataagaataa cactgtcgtt cgaattgtga gttgctaata atgttgcaaa
    27001 aatatgtgtt tcttgaacaa gttgaatatt aagacctatt cttgataact tttggtataa
    27061 gtcggtagca actacaaaag atgcgccaaa accatatata aaaatcgtct cagaacgttt
    27121 taaacaatga catatttgat caatagtttt atcatttaat tcgttattag catgattaag
    27181 tgcgcgtgta gtacgtgtat ggagtttagt tcttaaagat tctgtgcttt cgttattcat
    27241 taattcaaca ttgtaaattg atgatgcttt aggaacatat ttagatatat ttattttcaa
    27301 gtcgtgaaaa ccgccatcag taattttctt gctgaatcga atgactgatg atgtactaat
    27361 atctaataag cttgctaagt cttgagacct cattttaatc accttttgag gtgaatttag
    27421 tataaattta gctatctttt tttcattttt agtcatatat ggatattgac tatctatttc
    27481 tgttagtacg tttgacatat taatcactcc actttaacgc aatatatttt attagcgttg
    27541 gctattgttg tttaacgcga tggtatttac aacttgtaac gtaagagaat gaaaaataag
    27601 ctgaatatgt tgtttgttta atgatattga attataaaac tg
//
LOCUS       DAMZKC010000019        26932 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.019, whole genome shotgun sequence.
ACCESSION   DAMZKC010000019 DAMZKC010000000
VERSION     DAMZKC010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 26932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26932
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.019"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(319..489)
                     /locus_tag="POB55_000567"
     CDS             complement(319..489)
                     /locus_tag="POB55_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499553.1"
                     /translation="MLRLSIIFIAFIINTTITYGYTTEGTWVNLLFKSLSLSMIIVFM
                     FYYIRFVIEKKR"
     gene            complement(551..649)
                     /locus_tag="POB55_000568"
     CDS             complement(551..649)
                     /locus_tag="POB55_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499554.1"
                     /translation="MIKKKHLSGSLVPDRCFLIISEEVERFMENAY"
     gene            complement(668..1567)
                     /gene="sdaAA"
                     /locus_tag="POB55_000569"
     CDS             complement(668..1567)
                     /gene="sdaAA"
                     /locus_tag="POB55_000569"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HDI6499555.1"
                     /translation="MFDSIRETIDYAVENNMSFADIMVKEEMELSGKSRDEVRAQMKQ
                     NLDVMRDAVIKGTTGDGVESVTGYTGHDAAKLRDYNETHHALSGYEMIDAVKGAIATN
                     EVNAAMGIICATPTAGSSGTIPGALFKLEKTHDLTEEQMIDFLFTSALFGRVVANNAS
                     VAGATGGCQAEVGSASAMAAAAAVAIFGGSPEASGHAMALAISNLLGLVCDPVAGLVE
                     IPCVMRNAIGSGNALISADLALAGIESRIPVDEVIEAMDKVGRNLPASLRETGLGGLA
                     GTPTGEAIKRKIFGTAEDMVKNN"
     gene            complement(1581..2261)
                     /gene="sdaAB"
                     /locus_tag="POB55_000570"
     CDS             complement(1581..2261)
                     /gene="sdaAB"
                     /locus_tag="POB55_000570"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HDI6499556.1"
                     /translation="MAKSYDYQSAFDIIGPVMMGPSSSHTAGAVKIGNSARAILGDIP
                     KNIEIHYYESFAQTHQGHGTDVAIVGGAMGYSTFDNRIKSSLEIAADDGIKIDIIEEE
                     GDSIGQHPNCAYIKSSRNDGRYIEIIGISIGGGTIKIKGIHINGLEVDLNHGLPILVV
                     DGNMTKAQVNHFINDINDMKLDCKDEFIKIDEDKCLVVIPLNKAISESTLNTIKEKYS
                     DVNVSYIN"
     gene            complement(2264..3307)
                     /locus_tag="POB55_000571"
     CDS             complement(2264..3307)
                     /locus_tag="POB55_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDI6499557.1"
                     /translation="MDILLGVGTLVLVLVIMTLFLKYAPYGKQGLQALSGAACATFLP
                     QAFLSYAIGGVFDIKFLQDIGDLAGSLSGIAVGILTCLNLGVAPVFAVIVGLVLHDFK
                     LLPAFIAAYGVAFLIKWIEKKVPEGLDLIVVILFAPAIAFGLASIITPGVLATLKQIG
                     SAVTAVGDNNPYALAVILGLVIPVTGMTPLSSMVLTSLLGLTGVPMAIGALTCTGSSF
                     VNLILFRKLNIGGPSKAFAVCIEPLTQIDLIAQYPVQLYGTNALIGVVNACIVTFSGL
                     VIGVKGMATPIAGAIVLFGFNNAVTSIVTIATVIIVSIVLAYIIGTLINKFNLMNINF
                     KMPSKKNHIKESV"
     gene            3857..4060
                     /locus_tag="POB55_000572"
     CDS             3857..4060
                     /locus_tag="POB55_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499558.1"
                     /translation="MQLSHSVKVAISIYLALIFITFTSYLVIILYTSMTGHDVSHFVL
                     DSQHSHHGSLTQKHLSLPEISFK"
     gene            4243..5178
                     /locus_tag="POB55_000573"
     CDS             4243..5178
                     /locus_tag="POB55_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDI6499559.1"
                     /translation="MRYLANNHSALRLKGIILAIVGACLWGLGGTVSDFLFKYKNINV
                     DWYVTARLVVSGVFLLIMYKMMQPKRSIFSVFQDRRMLGKLLIFSILGMLVVQYAYMA
                     SINTGNAAIATLLQYIAPVYIIIWFVIRGVAKLTLFDVLAIIMTLLGTFLLLTNGSFS
                     NLVVNPASLFWGILAGVALAFYTIYPSDLLNRFGSILIVGWAMLISGVAMNLRHPIWH
                     IDITKWDISIILFLIFGIIGGTALAFYFFIDSLQYISAKETTLFGTVEPVVAVIASSL
                     WLHVAFKPFQIVGIILIMILILLLSLKRQPEALDE"
     gene            complement(5259..6227)
                     /locus_tag="POB55_000574"
     CDS             complement(5259..6227)
                     /locus_tag="POB55_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDI6499560.1"
                     /translation="MRKKWSTLAFGFLVAAYAHIRIKEKRSVKSYMLEQGIRLSRAKR
                     RFMYKEEAMKALEKMAPQTAGEYEGTNYQFKMPVTVDKHYGSTVYTVNDKQDKHQRVV
                     LYAHGGAWFQDPLKIHFEFIDELAETLNAKVIMPVYPKIPHQDYQATYVLFEKLYHDL
                     LNQVADSKQIVVMGDSAGGQIALSFAQLLKEKHIVQPGHIVLISPVLDATMQHPEIPD
                     YLKKDPMVGVDGSVFLAEQWAGDTPLDNYKVSPINGDLDGLGRITLTVGTKEVLYPDA
                     LNLSQLLSAKGIEHDFIPGYYQFHIYPVFPIPERRRFLYQVKNIIN"
     gene            complement(6360..6704)
                     /locus_tag="POB55_000575"
     CDS             complement(6360..6704)
                     /locus_tag="POB55_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDI6499561.1"
                     /translation="MVKQLNSVEAFREFIHQYPLAVVHVMRDQCSVCHAVLPQIEDLM
                     QSYPNVPLAVINQSQVEAIAGELNIFTVPVDLIFMNGKEMHRQGRFIDMQRFEHHLKQ
                     MNDSVNNDVDEH"
     gene            complement(6746..7156)
                     /locus_tag="POB55_000576"
     CDS             complement(6746..7156)
                     /locus_tag="POB55_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HDI6499562.1"
                     /translation="MTTEGLLVAEKEIEVNGYDIDAMGVVSNIVYIRWFEDLRTAFIN
                     QHMNYSTMINQGISPILMKTEAEYKVPVTIHDKPVGRIYLVKASKMKWVFQFEIVSAH
                     GVHCIGTQTGGFYRLSDKKITSVPQVFQDILATK"
     gene            complement(7521..9587)
                     /locus_tag="POB55_000577"
     CDS             complement(7521..9587)
                     /locus_tag="POB55_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIABC"
                     /protein_id="HDI6499563.1"
                     /translation="MFKKLFGQLQRIGKALMLPVAILPAAGILLAFGNAMHNEQLVEI
                     APWLKNDIIVMISSVMEAAGQVVFDNLPLLFAVGTALGLAGGDGVAALAALVGYLIMN
                     ATMGKVLHITIDDIFSYAKGAKELSQAAKEPAHALVLGIPTLQTGVFGGIIMGALAAW
                     CYNKFYNITLPPFLGFFAGKRFVPIVTSVVAIATGVLLSFAWPPIQDGLNSLSNFLLN
                     KNLTLTTFIFGIIERSLIPFGLHHIFYSPFWFEFGSYTNHAGELVRGDQRIWMAQLKD
                     GVPFTAGAFTTGKYPFMMFGLPAAAFAIYKNARPERKKVVGGLMLSAGLTAFLTGITE
                     PLEFSFLFVAPVLYGIHVLLAGTSFLVMHLLGVKIGMTFSGGFIDYILYGLLNWDRSH
                     ALLVIPVGIVYAIVYYFLFDFAIRKFKLKTPGREDEETEIRNSSVAKLPFDVLDAMGG
                     KENIKHLDACITRLRVEVVDKSKVDVAGIKALGASGVLEVGNNMQAIFGPKSDQIKHD
                     MAKIMSGEITKPSETTVTEEMSDEPVHVEALGTTDIYAPGIGQIIPLSEVPDQVFAGK
                     MMGDGVGFIPEKGEIVAPFDGTVKTIFPTKHAIGLESESGVEVLIHIGIDTVKLNGEG
                     FESLINVDEKVTQGQPLMKVNLAYLKAHAPSIVTPMIITNLENKELVIEDVQDADPGK
                     LIMTVK"
     gene            complement(9888..11627)
                     /locus_tag="POB55_000578"
     CDS             complement(9888..11627)
                     /locus_tag="POB55_000578"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HDI6499564.1"
                     /translation="MAKIKANEALVKALQAWDIDHLYGIPGDSIDAVVDSLRTVRDQF
                     KFYHVRHEEVASLAAAGYTKLTGKIGVALSIGGPGLIHLLNGMYDAKMDNVPQLILSG
                     QTNSTALGTKAFQETNLQKLCEDVAVYNHQIEKGDNVFEIVNEAIRTAYEQKGVAVVI
                     CPNDLLTEKIKDTTNKPVDTSRPTVVSPKYKDIKKAVKLINKSKKPVMLIGVGAKHAK
                     DELRAFVEAAKIPVVHSLPAKTILPDDHPYSIGNLGKIGTKTSYQTMQDADLLIMVGT
                     NYPYVDYLPKKNIKAIQIDTNPKNIGHRFNINVGIVGDSKIALHQLTENIKHVAERPF
                     LNKTLERKAVWDKWMEQDKNNNSKPLRPERLMASINKFIKDDAVISADVGTATVWSTR
                     YLNLGVNNKFIISSWLGTMGCGLPGAMASKIAYPNRQAIAIAGDGAFQMVMQDFATAV
                     QYDLPLTVFVLNNKQLAFIKYEQQAAGELEYAVDFSDMDHAKFAEAAGGKGYTIKSAS
                     EVDAIVEEALAQDVPTIVDVYVDPNAAPLPGKIVNEEALGYGKWAFRSITEDKHLDLD
                     QIPPISVAAKRFL"
     gene            complement(11674..12363)
                     /locus_tag="POB55_000579"
     CDS             complement(11674..12363)
                     /locus_tag="POB55_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HDI6499565.1"
                     /translation="MNDYVQALLMILLTVVLYYFAKRLQQKYPNPFLNPALIASLGII
                     FVLLIFGISYNGYMKGGSWINHILNATVVCLAYPLYKNREKIKDNVSIIFASVLTGVM
                     LNFMLVFLTLKAFGYSKDVIVTLLPRSITAAVGIEVSHELGGTDTMTVLFIITTGLIG
                     SILGSMLLRFGRFESSIAKGLTYGNASHAFGTAKALEMDIESGAFSSIGMILTAVISS
                     VLIPVLILLFY"
     gene            complement(12356..12751)
                     /gene="cidA"
                     /locus_tag="POB55_000580"
     CDS             complement(12356..12751)
                     /gene="cidA"
                     /locus_tag="POB55_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin-like murein hydrolase modulator CidA"
                     /protein_id="HDI6499566.1"
                     /translation="MHKVQLIIKLLLQLGIIIVITYIGTEIQKIFHLPLAGSIVGLFL
                     FYLLLQFKIVPLTWVEDGANFLLKTMVFFFIPSVVGIMDVASEITLNYILFFAVIIIG
                     TCIVALSSGYIAEKMSVKHKHRKGVDAYE"
     gene            complement(12964..13842)
                     /locus_tag="POB55_000581"
     CDS             complement(12964..13842)
                     /locus_tag="POB55_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI6499567.1"
                     /translation="MDIKHMKYFIEVVKQGGMTNASKSLYIAQPTISKAIKDIENEMG
                     TPLFDRSKRHLILTDAGQIFYEKSKEIVALYDYLPSEMERLNGLETGHINMGMSAVMN
                     MKILINILGAFHQQYPNVTYNLIENGGKTIEQQIINDEVDIGVTTLPVDHHIFDYTTL
                     DKEDLRLIVSREHRLAKYETVKLEDLAGEDFILFNKDFYLNDKIIENAKNVGFVPNTV
                     AQISQWHVIEDLVTNELGISILPTSISEQLNGDVKLLRIEDAHVHWELGVVWKKDKQL
                     SHATTKWIEFLKDRLG"
     gene            14009..14227
                     /locus_tag="POB55_000582"
     CDS             14009..14227
                     /locus_tag="POB55_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterile alpha motif-like domain-containing
                     protein"
                     /protein_id="HDI6499568.1"
                     /translation="MSFYEFMQSFLGDDTPLGELVDWINQDSNFPREVKSHSEILSYF
                     RTNPCPESISVPVIKRALSVFNQFTNVQ"
     gene            14617..15048
                     /locus_tag="POB55_000583"
     CDS             14617..15048
                     /locus_tag="POB55_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDI6499569.1"
                     /translation="MEYKKILIRLLIAFAVLFSADFTYQSVEQTHQSHAAVNYYSKNQ
                     CTWWAFKRRAQVGKPVSNRWGNAKNWYYNARKSKYATGRTPRKFAVMQSTAGYYGHVA
                     VVEQVYKNGSIKVSEYNFYRPLKYNTRVLSKKAARNFNYIY"
     gene            complement(15186..16466)
                     /locus_tag="POB55_000584"
     CDS             complement(15186..16466)
                     /locus_tag="POB55_000584"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HDI6499570.1"
                     /translation="MQNLDKNFRHLSRKEKLQQLVDKQWLSEEQFDILLNHPLIDEEV
                     ANSLIENVIAQGALPVGLLPNIIVDDKAYVVPMMVEEPSVVAAASYGAKLVNQTGGFK
                     TVSSERIMIGQIVFDGVDDTEKLSADIKALEKQIHKIADEAYPSIKARGGGYQRIAID
                     TFPEQQLLSLKVFVDTKDAMGANMLNTILEAITAFLKNEFPQSDILMSILSNHATASV
                     VKVQGEIDVKDLARGERTGEEVAKRMERASVLAQVDIHRAATHNKGVMNGIHAVVLAT
                     GNDTRGAEASAHAYASRDGQYRGIATWRYDQDRQRLIGTIEVPMTLAIVGGGTKVLPI
                     AKASLELLNVESAQELGHVVAAVGLAQNFAACRALVSEGIQQGHMSLQYKSLAIVVGA
                     KGDEIAKVAEALKKEPRANTQAAERILQDLRSQQ"
     gene            16720..17886
                     /locus_tag="POB55_000585"
     CDS             16720..17886
                     /locus_tag="POB55_000585"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HDI6499571.1"
                     /translation="MTIGIDKINFYVPKYYVDMAKLAEARQVDPNKFLIGIGQTEMAV
                     SPVNQDIVSMGANAAKDIITDEDKKKIGMVIVATESAVDAAKAAAVQIHNLLGIQPFA
                     RCFEMKEACYAATPAIQLAKDYLATRPNEKVLVIATDTARYGLNSGGEPTQGAGAVAM
                     VIAHNPSILALNEDAVAYTEDVYDFWRPTGHKYPLVDGALSKDAYIRSFQQSWNEYAK
                     RQGKSLADFASLCFHVPFTKMGKKALESIIDNADETTQERLRSGYEDAVDYNRYVGNI
                     YTGSLYLSLISLLENRDLQAGETIGLFSYGSGSVGEFYSATLVEGYKDHLDQAAHKAL
                     LNNRTEVSVDAYETFFKRFDDVEFDEEQDAVHEDRHIFYLSNIENNVREYHRPE"
     gene            complement(18056..18577)
                     /locus_tag="POB55_000586"
     CDS             complement(18056..18577)
                     /locus_tag="POB55_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDI6499572.1"
                     /translation="MEYKSYYDSPVGRLELVSDGGSLTAVLFENQQGDSTREENTSLA
                     IFREATQWLDAYFKGDNPEITIPLKPTGSHFQQCVWNELRQVPYGTLTTYGAIAKKVG
                     KLLNKPKMSAQAVGGAVGSNPLSIIVPCHRVVGKTGSLTGFGGTINNKIKLLELENID
                     MSKLYVPKHSTKP"
     gene            18986..21091
                     /gene="clpL"
                     /locus_tag="POB55_000587"
     CDS             18986..21091
                     /gene="clpL"
                     /locus_tag="POB55_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpL"
                     /protein_id="HDI6499573.1"
                     /translation="MNNGFFNSDFDSIFRRMMQDMQGSNQVGNKKYYINGKEVSPEEL
                     AQLTQQGGNHSAEQSAQAFQQAAQRQQGQQGGNGNYLEQIGRNLTQEARDGLLDPVIG
                     RDKEIQETAEVLSRRTKNNPILVGEAGVGKTAIVEGLAQAIVEGNVPAAIKDKEIISV
                     DISSLEAGTQYRGAFEENIQKLIEGVKSSQNAVLFFDEIHQIIGSGATGSDSGSKGLS
                     DILKPALSRGEISIIGATTQDEYRNNILKDAALTRRFNEVLVNEPSAKDTVEILKGIR
                     EKFEEHHQVKLPDDVLKACVDLSIQYIPQRLLPDKAIDVLDITAAHLSAQSPAVDKVE
                     TEKRISELENDKRKAVSAEEYKKADDIQNEIKSLQDKLENSNGEHTAVATVHDISDTI
                     QRLTGIPVSQMDDNDIERLKNISNRLRSKIIGQDQAVEMVSRAIRRNRAGFDDGNRPI
                     GSFLFVGPTGVGKTELAKQLAIDLFGNKDALIRLDMSEYSDTTAVSKMIGTTAGYVGY
                     DDNSNTLTEKVRRNPYSVILFDEIEKANPQILTLLLQVMDDGNLTDGQGNVINFKNTI
                     IICTSNAGFGNGNDAEEKDIMHEMKKFFRPEFLNRFNGIVEFLHLDKDALQDIVNLLL
                     DDVQVTLDKKGITMDVSQDAKDWLIEEGYDEELGARPLRRIVEQQVRDKITDYYLDHT
                     DVKHVDIDVEDNELVVKGK"
     gene            complement(21160..21330)
                     /locus_tag="POB55_000588"
     CDS             complement(21160..21330)
                     /locus_tag="POB55_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HDI6499574.1"
                     /translation="MSVIINILIFLAIFGYALYTLVKFFKRSKQGKCGTCDINRDCCG
                     TEQHTANHFPGK"
     gene            complement(21343..23337)
                     /gene="feoB"
                     /locus_tag="POB55_000589"
     CDS             complement(21343..23337)
                     /gene="feoB"
                     /locus_tag="POB55_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HDI6499575.1"
                     /translation="MENYCILGNPNVGKTSLFNALTGSYEYIGNWSGVTVEKKVGKLK
                     ENVGQLIDLPGTYDLSPISKDETVVTDYLLNDSFSGIINIVDASQLKRNMQLTVQLLE
                     LNQPIYIGLNMIDVATKRGIKIDYHKLMKKLKTPIFPVVARTGKGTKHLLGEIKHLGE
                     GYQPHFKINYGEKIEETIKNMCQIIMTETSHDKYQARFIAIQFLLNNMQIANELNSEV
                     VNKLSSLRDQVDKQVEAVSVRREMERIRNHYIETLLQDVVTYPDEDKQYFSSRIDKIL
                     THKYIGMPIFLAIMWLIFQTTFTWIGTPLSDQLDAFIGGTFTDSVKTIMNYLGVIPFL
                     QDLITDGIIAGVGSVLVFVPQIVVLFFFISLLEDSGYMARIAVLMDRIMESFGLSGKS
                     FIPMIIGFGCNVPSIMAARSIENEKERLTTILIAPFMSCSARLPVYALFVGIFFKENQ
                     SLVVLSLYVLGIIMAFLVSTVLTKTILKNDNAIFIVELPTYRVPSIKTLWRSTWEKAK
                     GFVRKAGTFIFGGSVVIWLLSYVGPHGINVNINQSFLHMVGSFFGMLVQPLGFGTWQA
                     GATLVPGFLAKEVIVSSMAIIYSSGDAGLVNVIQNQFTPLSAYAFMIFILLYIPCVST
                     VAAIRKETYSWKWTALAVVYPLVTAYVLTFIFYQIGHLFV"
     gene            complement(23349..23576)
                     /locus_tag="POB55_000590"
     CDS             complement(23349..23576)
                     /locus_tag="POB55_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDI6499576.1"
                     /translation="MLNIKNGEMNKAYKIKRMDIANENMLYRLSAFGLTDDAIITIKQ
                     KCLFKGPCIIEVNGQQLSIRHCDACSIALEE"
     gene            complement(23792..26281)
                     /gene="farE"
                     /locus_tag="POB55_000591"
     CDS             complement(23792..26281)
                     /gene="farE"
                     /locus_tag="POB55_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid efflux MMPL transporter FarE"
                     /protein_id="HDI6499577.1"
                     /translation="MAKFLYKMGTFIAKHKWSAVIAWIVIVAAILIPLATNAPKFDND
                     IKMTGLESLDTNKKIEKHFNQDSEKAQIRVVFKTTKDDGIVQPNITKDIKKTLDDIKK
                     DDKHIDKISDPYENKQISKDKTTAFADITYDVSQTSLKDGSRDNVKSHLKDLRDNHNV
                     QTELTGTGMTSTEVGGNSELVGIIVAFVVLLITFGSVIAAGLPIISALIGLASGVGII
                     SLLTYAFDIPNVTLTLAVMIGLAVGIDYALFILFRYRQVMKTETDYIKGIGLAIGTAG
                     SAVIFAGVTVVIAVCGLSLVGIDFLAVMGFASAISVIFAVFSALTLLPALISIFHKRI
                     KVNKLQSNFKKDIDTPWSKFITGNALAAVLLGLIILVAAAIPVSHMRLGIPDDGVKPA
                     DSTQKKAYDIISDKFGEGFNGQIPMLINVKDKKDDPQGLQQDLQSVYKDIKDKKNVDI
                     VTPPQMSKDNDYALMVVIPKKGPNAESTNDLVHDLRDYHKDAQDKYGFKTEISGQSVI
                     NIDMSKKLNEAIPLFATVIVVLAFFLLMIVFRSILIPLKAVLGFVLSLMATLGFTTLV
                     MQDGFMKGLFGIETTGPMLAFLPVITIGILFGLAMDYEVFLMSRIHEEYSKTGDNDYS
                     IKVGLKESGPVIVAAALIMFSVFFAFVFQEDVMIKSMGMALAFGVLFDAFVVRMMLIP
                     ALTKLFGKGSWYLPAWLNRIIPRVDIEGHALEKYKTVESQESEAKDSKETYDTTFKIY
                     PQGATNVSKHQDVHGQDDVRSIVLDDKTMALYQEVKQQSASSFFLYDALIDYQNKHQL
                     NSKQQVTNIEQLNKNIEKLNQLLEKNLRNKS"
     gene            26426..>26932
                     /gene="farR"
                     /locus_tag="POB55_000592"
     CDS             26426..>26932
                     /gene="farR"
                     /locus_tag="POB55_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid efflux pump transcriptional regulator
                     FarR"
                     /protein_id="HDI6499578.1"
                     /translation="MKETDLRVIKTKKALSSSLLQLLEQQLFQTITVNQICDNALVHR
                     TTFYKHFYDKYDLLEYLFNQLTKDYFARDISDRLNHPFQTISDTINNKEDLRDIAEFQ
                     EEDAEFNKVLKNVCIKIMHNDIKNNRDRIDIDSDIPDNLIFYIYDSLIEGFMHWIKDE
                     KIDWPGEEI"
BASE COUNT         8573 a         5160 c         4116 g         9083 t
ORIGIN      
        1 gtcccacccc aacttacatt gtctgtagaa attgggaatc caatttctct ttgttggggc
       61 cccgccaact tgcacattat tgtaagctga tttttcgtca gcttctgtgt tggggcccac
      121 accccaactc gcattgcctg tagaatttca tttcgaaatt ctctgtgttg gggcccctga
      181 ctagaatcga aaaaagcttg ttacaagcgc attttcgttc agtcaactac tgccaatata
      241 actttgtaga gcatattaca ttgatttaca tcgtcccttt tatttattct tttcaaatac
      301 tatccccata gctttgattt aacgcttttt ctcaataaca aaacgaatat agtagaacat
      361 gaaaacgata atcatgctga gcgataaaga tttaaataat agattgaccc acgttccctc
      421 agtcgtatat ccatatgtaa tcgttgtgtt aatgatgaat gctataaaga tgattgatag
      481 tcttagcata tcatcactcc ttttaagtta ttttagatat acggggacgc ttttgcaatc
      541 actattttga ttagtatgca ttttccataa atctttcaac ttcttcagag ataattaaga
      601 agcatctatc tggtactaat gatccagaca gatgcttctt ttttatcaat attttattgt
      661 tatctcatta attattttta accatatctt cagctgtgcc aaagatttta cgtttaattg
      721 cttcgccagt tggtgtgcct gctagtccac ccaatccagt ttcacgtaat gatgcaggaa
      781 ggttacgacc aaccttatcc attgcttcaa taacttcatc aacagggatt ctactttcaa
      841 tacctgctaa tgctaaatct gctgaaatta aagcgttacc cgaaccaatt gcatttctca
      901 taacacaagg aatttcaaca agtccggcta ctggatcaca aactaaacct aataaattac
      961 ttatcgctaa tgccatagcg tgcccggatg cttctggtga tcctccgaat atagctactg
     1021 ctgcagctgc ggccattgca gatgctgaac caacttcagc ttggcagcca cctgttgcac
     1081 cagctacact tgcattgttt gctacgacac gcccaaacaa tgctgaagtg aataagaaat
     1141 caatcatttg ctcttctgtt aaatcatgtg ttttttctaa tttaaaaagt gcaccgggaa
     1201 tggtacccga ggaaccagct gttggcgttg cacaaataat acccatcgca gcattgactt
     1261 catttgttgc aatggcacct ttgactgcgt caatcatttc atatccagac aaagcatgat
     1321 gtgtttcatt ataatcacgt agtttagcag catcatgacc agtgtagccc gttacacttt
     1381 caaccccatc acctgtcgtc cctttgatta ctgcgtctcg catgacatct aaattttgtt
     1441 tcatttgcgc tcgcacttca tcacgtgatt taccgcttaa ttccatttct tctttaacca
     1501 tgatatccgc aaatgacata ttattttcta cggcataatc tatagtctct ctaattgaat
     1561 caaacatgtt tattccccct ctaatttata taggaaacgt ttacgtcact gtatttctct
     1621 ttaattgtat ttaatgttga ttctgagatt gctttattta atggtattac aaccaagcat
     1681 ttatcttcat ctatcttaat aaattcatct ttacagtcta atttcatatc gttgatatca
     1741 ttgatgaaat gatttacttg tgctttagtc atatttccgt caacaactaa aattggtaat
     1801 ccatgattta aatctacttc tagtccattt atatgaatac ctttaatttt aattgtacca
     1861 ccaccgattg aaataccgat aatttcaatg tagcgaccat cattacgaga tgatttgata
     1921 taagcacagt ttggatgttg accaatacta tcgccttctt cttcgatgat atctatttta
     1981 ataccatcat cagctgcaat ttctaatgaa gatttaattc ggttatcaaa tgttgaatat
     2041 cccattgctc cacccacaat agcgacatct gtaccatgtc cttggtgtgt ttgagcaaat
     2101 gattcataat aatgtatttc aatattttta ggaatatctc ccaatattgc gcgtgctgaa
     2161 ttcccaatct ttactgcacc agccgtatga gaacttgaag ggcccatcat aactggtccg
     2221 ataatatcga aagcactttg ataatcatag ctctttgcca taattaaaca ctctccttaa
     2281 tatgattctt tttgctcggc attttaaagt tgatattcat taaattaaac ttattaatta
     2341 gtgttccaat aatataggct aagacaatgc tgacaataat cactgttgcg attgttacaa
     2401 ttgatgtcac tgcattatta aagccaaaca atacgatggc gcctgcaatt ggtgttgcca
     2461 tacctttgac acctattact agtccgctaa atgtcacgat acatgcgttg acgaccccaa
     2521 tcagtgcatt tgtaccatat agttgtactg gatattgcgc tattaaatca atttgcgtca
     2581 atggctcaat acaaactgca aatgcttttg acggtccacc aatgtttaat tttcggaata
     2641 aaataaggtt aacaaatgag ctacctgtac atgttagtgc tccaatagcc ataggaacac
     2701 ctgtcagtcc taataaactt gttaatacca ttgaacttag cggtgtcata cctgtaacag
     2761 gaatcactag tcctaaaatg accgctaatg catatggatt gttatcacct accgcagtga
     2821 cagcactacc tatttgtttt aatgttgcta gcacaccagg tgtaatgatt gatgcaagtc
     2881 cgaaagcaat tgctggtgca aataagatca ccacaattaa gtccaagcct tctggaactt
     2941 tcttttcaat ccatttaatt aaaaaagcta cgccataagc tgcgatgaat gctggtaata
     3001 atttaaagtc atgtaatact aaaccaacaa taaccgcaaa tactggtgca acgcctaagt
     3061 ttaagcacgt tagaatacct actgcgatac cgcttaaact tcctgctaaa tccccaatat
     3121 cttgtagaaa tttaatatca aatacgccac caatagcgta acttaagaat gcttgtggta
     3181 gaaatgtcgc acaagctgca ccggataatg cttgtagtcc ttgtttaccg tacggtgcat
     3241 actttaaaaa tagcgtcatg atcactaaaa ccaagactaa tgtgcctaca cctaacagaa
     3301 tatccatttc ccaaaaacct ctctctatgt ttattttatt ttcagaccat aaacatcgta
     3361 cacccttaag aaaacgtttt caacttttat ctgttatcaa atcaaatatt taagtgaaat
     3421 atttcatatt tgtgaaagat ttttaaaatg gattgtttca aaaactactt atgttgtcat
     3481 taatatttac taattaactt tttactctat atttcaaaca gttgtgtgac agttttttga
     3541 taactttttt acatctgaaa gtgagtaata tttctaaaaa tctttaatat ttatacactt
     3601 tatctttcga actatttatt ttttgttttt gtgctgtgtt tataaaaccg cacactatat
     3661 tcaaaaataa cttattacac atatatttgc aataagataa ttaaagtagg atattatttt
     3721 tagttttctg ataggaatga tgatagtcta taggttgaat ctttactttt tttaaagcta
     3781 aatttacatc aacttaacaa tggctggtta tactgaagat gaaaatattt agtataactt
     3841 agtggaggcg ataaaggtgc aattgagtca ttccgttaaa gttgcaattt ctatctattt
     3901 agcacttatc tttataacgt tcacttctta tttagtcatt attttatata cgagtatgac
     3961 tggacatgat gtatcacatt tcgtgttaga tagtcagcat tctcatcatg gatctcttac
     4021 gcaaaaacat ttgagtcttc ctgaaatctc atttaaatag tttatcctct gtgtttcaac
     4081 attcatttcc catatcgatt catttatcta tcatctagac cactacatct tagatgattt
     4141 ttttattttc tcatttcact ctttcttaaa agtcgatata atgaattaaa tcattatcat
     4201 acaccgacat attttatgtt gttggtgtta agttttaaag gggtgagata cttggcgaat
     4261 aatcattcag ctttgcgttt aaaaggaatt atacttgcca ttgtcggtgc ttgtttatgg
     4321 ggattaggtg gtactgtttc tgatttcttg ttcaaatata agaatattaa tgtcgattgg
     4381 tacgtcactg ctcgacttgt agtcagtggt gttttcttac ttattatgta caaaatgatg
     4441 caacccaaac gttctatatt tagcgtattc caagatcgac gtatgttagg caaattactt
     4501 atcttcagta tactgggcat gttagttgta caatatgctt atatggcatc tattaataca
     4561 ggtaatgctg cgattgcaac attactacaa tacattgcgc cagtttatat tattatttgg
     4621 tttgtcataa gaggcgttgc aaaactaaca ttatttgatg tgcttgctat tatcatgaca
     4681 ctattaggaa catttttatt attaacgaat ggttcatttt ctaatttagt cgtcaatcct
     4741 gcaagtttat tctggggtat tttagctggt gtagcactcg ctttttacac aatttatcct
     4801 tcagacctac ttaaccgctt cggttcgatt ctaattgtcg ggtgggcaat gcttatttct
     4861 ggtgttgcga tgaatttacg ccatccaatt tggcacattg atatcactaa atgggacata
     4921 tcaattatat tatttttaat ctttggtatt atcggtggta ccgcactcgc attttatttc
     4981 tttatcgaca gtttacaata catatcagcg aaagaaacaa cattattcgg aactgttgaa
     5041 cctgtcgtag ccgttatcgc aagcagtcta tggttacatg tggcattcaa accatttcaa
     5101 atcgtaggca tcattcttat tatgatttta attttattac tatcacttaa aagacaacct
     5161 gaagcattag atgaataaga aaactctgat aatcacttta gcaagtaact atttttaaca
     5221 acgtagctac cttttatgtg atatcagagt tttttatttt aattaataat atttttcact
     5281 tggtataaaa agcgtcggcg ctctggtatc ggaaatactg gataaatatg gaattggtaa
     5341 tatccaggta taaagtcatg ttcgatacct tttgcactca acaattgcga taagttcaaa
     5401 gcatctgggt acagtacttc ttttgtgcca acagttaatg taatacgacc taaaccatct
     5461 aagtcaccat taattggtga tactttgtag ttatctaaag gtgtgtcccc tgcccattgt
     5521 tcagctaaga acacactgcc atccacacct accattgggt ctttctttaa gtagtcagga
     5581 atttcaggat gctgcatcgt tgcatctaaa actggtgaaa ttaatacaat atgtcctggt
     5641 tgcacaatat gtttttcttt taacaattga gcaaatgata aagcaatttg accgcccgca
     5701 gagtcaccca ttacaacgat ttgtttagaa tctgctactt gattcaataa atcatggtat
     5761 aacttttcaa aaagcacata cgtcgcttga taatcttgat gcggaatctt cggatatact
     5821 ggcatgatga ctttagcatt gagtgtttct gcaagttcat caataaattc gaagtgaatt
     5881 ttaagtgggt cttggaacca tgcacctcca tgtgcgtata atacaacacg ttgatgctta
     5941 tcttgtttat cattaacggt ataaacggtt gaaccgtagt gcttatccac tgttactggc
     6001 atcttaaact gataattggt gccctcatat tcacctgctg tctgtggcgc cattttttct
     6061 aatgctttca tcgcttcttc tttatacata aaacgacgct tagctctaga taatcgtata
     6121 ccttgttcta acatataact tttcacactg cgtttttctt taattctaat atgtgcgtat
     6181 gctgctacta aaaatccaaa cgcaagtgta gaccattttt tacgcatttg tcgtcactcc
     6241 tctttgatga tttctactat agttctactg taccatgctg attcttatta ctaccaatat
     6301 ttactgggaa ataagttatg atgtctacat aatcttagca atgctaatca tttgcgatat
     6361 taatgctcat cgacatcgtt atttacacta tcattcattt gcttaagatg atgttcaaaa
     6421 cgttgcatat cgataaaacg cccttgacga tgcatttctt ttccattcat aaaaattaaa
     6481 tccacaggta cagtgaaaat atttaattct ccagcaatag cttccacctg actttgatta
     6541 atcacagcta atggcacatt gggatatgat tgcatcaagt cttcaatttg tggtaaaacg
     6601 gcatgacaca cgctacactg atcgcgcatg acatgtacaa ctgctaacgg atattgatga
     6661 ataaattcac ggaatgcttc gacactattt aattgcttaa ccatttcgaa caccttctta
     6721 ctttttatat tttaaaatga agtcattatt ttgttgctaa aatgtcttga aacacttgtg
     6781 gcacagaggt tatcttctta tcgctcaatc tgtaaaaacc gcctgtctgt gtaccaatac
     6841 aatgcacgcc atgtgcggac acaatttcaa actgaaacac ccatttcatc ttgcttgctt
     6901 taactaagta aatacgacct actggtttgt catgtattgt gacaggtact ttatactctg
     6961 cttccgtttt cataagtata ggtgaaatgc cttgattgat cattgttgag taattcatgt
     7021 gctgattaat aaacgctgtt ctcaaatctt cgaaccatct aatataaacg atattactaa
     7081 cgacacccat cgcatcaatg tcgtaaccat tcacttcgat ttctttctct gcaactaata
     7141 gaccttctgt tgtcataatg acaaatctcc ttatcttaca aatagattta aaatcatgtt
     7201 aatttatttt tattactttc atgataaaaa tacaagcaat tgaactttat tacggaaaat
     7261 caaacttttc aatgacgtta ttaatttgac tagtcgtgaa gatgcatttt tctaaaatgt
     7321 acaaaaagta caatccctac tttctatccc ccaaactaac atctatgcca ttgctataca
     7381 tcattgcgca ctaaaaaggc agtagcttca ttttaagcta ctgccttttg taacgtattc
     7441 tctttcaact cgctacttcg catacgcact acgaatgact aaaagttcat tcatttgata
     7501 tgctgtttca tttttaatca ttatttgact gtcataatta gcttacctgg atcagcatct
     7561 tgtacatctt caatgacaag ttctttattt tcaagatttg taataatcat tggtgtaacg
     7621 atgcttggtg cgtgtgcttt caagtatgct aaattcactt tcattaatgg ttgaccttgt
     7681 gttacttttt catcaacgtt aatcagactt tcgaatcctt caccattcag tttcactgta
     7741 tcgataccaa tatgaataag tacttcgacg ccactttcag attctaatcc tatcgcatgt
     7801 ttcgtaggga agattgtttt cactgtacca tcaaacggtg ctacaatttc acctttttca
     7861 gggataaagc caacaccatc acccatcatt ttaccagcga atacttgatc aggtacttct
     7921 gataatggaa tgatttgacc gatacctggt gcatagatgt ctgttgttcc aagtgcttct
     7981 acgtgaactg gttcatctga catttcttca gtcactgtcg tttcacttgg tttcgtaatt
     8041 tcaccactca taatcttggc catatcatgt ttaatttgat ctgattttgg accaaagata
     8101 gcttgcatat tgtttccaac ttctaataca cctgatgcgc ctaaagcttt aatacctgct
     8161 acatctactt ttgatttatc aaccacttct acgcgtagac gtgtaataca tgcatctaaa
     8221 tgtttaatgt tttcttttcc acccattgca tctaagacat caaatggtaa ttttgcgaca
     8281 ctagagttac gaatttcagt ttcttcatct tcacgacctg gtgttttcaa tttaaactta
     8341 cgaattgcaa agtcgaataa gaagtaatac acgatagcat atacaatacc gactggaata
     8401 actaataatg cgtgtgaacg atcccagttt aataaaccat ataaaatata atctatgaaa
     8461 ccacctgaga atgtcatacc aattttaacg cctaataaat gcattactaa gaatgatgta
     8521 ccagctaata atacgtgaat tccataaagt actggtgcta caaataagaa tgaaaattct
     8581 aatggctcag tgataccagt taaaaatgca gttaatcctg ctgataacat taaaccaccc
     8641 acgacttttt tacgttctgg tcgtgcattt ttataaatag caaatgccgc cgctggtaaa
     8701 ccaaacatca taaatggata tttaccagta gtaaatgcac cagcagtaaa tggtacgcca
     8761 tctttcaatt gtgccatcca aatacgttgg tcaccacgaa ccaattcacc tgcgtgattt
     8821 gtataacttc cgaattcaaa ccagaacggt gaatagaaaa tatgatgtaa accaaatgga
     8881 attaatgagc gttcaataat accgaatata aacgttgtta atgttaaatt tttatttaat
     8941 aagaaattcg ataaactatt taatccatct tgaattggtg gccacgcaaa gcttaaaagc
     9001 acacctgttg cgattgctac gaccgatgtc acaatcggta caaatcgttt acctgcaaag
     9061 aatcctaaaa atggtggtag tgtaatatta taaaatttgt tgtaacacca tgcggctaaa
     9121 gcacccatga taataccacc aaacacaccc gtttgtaacg ttggaatacc taatactaaa
     9181 gcatgtgctg gttctttcgc tgcttgactt aattcttttg cccctttggc atatgagaaa
     9241 atgtcatcaa ttgtaatgtg caacactttc cccattgttg cattcataat taagtaacct
     9301 actagcgctg ctaatgctgc aacaccgtct cctcctgcta atccaagtgc tgtaccaact
     9361 gcaaataata atggcaagtt atcaaataca acttgtcctg ctgcttccat gaccgacgaa
     9421 atcattacaa tgatatcgtt ttttaaccat ggtgcaattt ctactaattg ttcgttgtgc
     9481 attgcgttac caaacgctaa taaaatacca gctgctggta aaatcgcaac aggtaacatt
     9541 aatgctttac cgatacgttg caattgtcca aacaatttct taaacatata aacatcccct
     9601 atctcaattt ccgcttccat gtacaaaaaa agcaggcatg aacaaacaag actattctca
     9661 tagtgcggtt gtttgctcat gcctgcatta ccagtaacac gcaagtgata tctaattcat
     9721 agctattata catatttgta aaagcgtttt caacaacttt ttcacaattt tatcatcaat
     9781 tcagtacgat catttgtgat aaataaacaa aacggcaatt cccattttgt tgaaatagaa
     9841 attaccgttt ttatagttca attcaattgt gataaccttt aaatcagtta taagaaacgt
     9901 tttgctgcca ctgaaattgg tggaatttga tctaaatcta aatgcttatc ttcagtaatt
     9961 gatctaaatg cccacttacc ataaccaagc gcttcttcat ttacaatttt acctggtaat
    10021 ggcgcagcat taggatcaac atatacatct acaatcgttg gtacatcttg tgctaatgcc
    10081 tcttcgacta tagcatctac ttcgctagca ctcttaattg tataaccttt accacctgct
    10141 gcctcagcaa attttgcatg atccatatca gaaaaatcaa ctgcatattc taattcacca
    10201 gctgcttgtt gttcatattt aataaatgct aactgtttgt tattaagtac aaatacagtt
    10261 aaaggtaaat catattgtac tgctgtagcg aagtcttgca ttaccatttg gaatgcaccg
    10321 tcaccagcaa tggcaatcgc ttgtctattt ggatatgcaa tttttgatgc catagctcct
    10381 ggaagaccgc aacccattgt acctaaccaa cttgaaatga tgaacttgtt gttcacacca
    10441 agatttaaat aacgtgtaga ccaaactgtt gcagtaccaa catctgctga aattactgca
    10501 tcatctttaa taaatttgtt aattgatgcc attaaacgtt ctggacgtaa tggtttgcta
    10561 ttattatttt tatcttgttc catccattta tcccaaaccg ctttacgttc taacgttttg
    10621 tttaaaaatg gtctttccgc aacatgttta atattttcag ttaactgatg caatgcaatt
    10681 ttactgtctc caacaattcc tacattaata ttaaaacgat gtccgatatt tttaggattt
    10741 gtgtcgattt ggattgcttt aatatttttc ttaggtaagt aatctacata tggatagttt
    10801 gtacctacca taattagtaa atctgcatcc tgcattgtct gatatgaggt ttttgtacct
    10861 attttaccta agttaccaat actgtatgga tggtcatccg gtaagattgt cttagcaggt
    10921 aatgaatgaa cgaccggaat ttttgccgct tcaacaaacg cacgtagctc atctttcgca
    10981 tgttttgcac caacaccaat caacataacc ggctttttac tcttattgat taatttaacc
    11041 gcttttttaa tgtctttata ttttggtgat acaacagttg gtcttgatgt atctactggt
    11101 ttattcgttg tatctttaat tttttcagtt aataagtcgt taggacaaat aacaacagct
    11161 acaccttttt gttcatatgc cgtacgaatt gcttcgttaa caatttcaaa cacattgtca
    11221 cctttttcaa tttggtgatt ataaacggct acatcttcac ataatttttg taaatttgtt
    11281 tcttggaatg ctttcgttcc aagtgctgta ctattcgttt gtccagataa tattaattgc
    11341 ggtacattat ccattttggc atcatacata ccatttaata aatgaattaa accagggcca
    11401 ccgatactta atgccacacc gattttacca gttaattttg tgtaaccagc agccgctaag
    11461 cttgctactt cttcatgacg tacatgataa aatttaaatt gatctctcac tgtacgtaaa
    11521 ctatcgacta ctgcgtcgat tgagtctcct ggaataccat acaagtgatc tatatcccat
    11581 gcttgtaatg ctttaactaa tgcttcattt gcttttattt ttgccattat tactaatagc
    11641 ctccctttct gtctatcatt ttaaatatct aaattaatag aataataaaa ttagaacagg
    11701 tattaacact gaactaataa ctgcagttaa aatcatccca attgaactaa atgcaccgga
    11761 ttcaatatcc atttctagtg ctttagctgt gccaaatgca tgtgacgcat tcccatacgt
    11821 taaacctttg gcgatagaag attcaaatct tccaaatctt aataacatcg aacctaaaat
    11881 actaccgatt aaacccgttg tgataataaa aagtacggtc atcgtatctg taccacctag
    11941 ttcatgtgac acttcgatac ctactgcggc tgttatagat cggggcaata acgttacaat
    12001 gacgtcttta gaatagccaa atgctttaag tgttaagaac actaacatga aattcagcat
    12061 gacgccagtt aatacacttg caaaaatgat agagacattg tctttaattt tctctctatt
    12121 tttataaagt gggtacgcta aacatacgac cgttgcgttt aaaatatggt tgatccaact
    12181 gccacctttc atatacccgt tataactaat tccaaagata agtaagacaa aaataattcc
    12241 taaagatgca attaatgctg gattcaaaaa tgggttcgga tatttttgtt gtaacctttt
    12301 agcgaaataa tataaaacga cagtcaacaa aatcattaat aaggcttgca cgtaatcatt
    12361 cataagcgtc tacaccttta cgatgtttat gtttaacaga cattttttca gcaatataac
    12421 ctgaagataa tgcaacgata catgttccta tgataatgac tgcgaaaaag agtatataat
    12481 ttagcgtaat ttcggaagca acatccataa tacctacaac tgacggtatg aagaaaaaga
    12541 ccatcgtctt taataaaaag tttgcaccgt cttctaccca agttagcggt acaatcttaa
    12601 attgtaatag taaataaaat aaaaatagac caacaatact gccggctaag ggaagatgaa
    12661 aaatcttttg aatttctgtg ccaatataag taatcacaat gatgattcct agttgtagta
    12721 gtagtttgat tattaattgg actttgtgca tggcgccatc cctttctaaa tatgtctaaa
    12781 ttgttacaat aactattata aagatggcgt acacaatata aaaatactct ttttttatag
    12841 tttgtattac tattttctat ggaaacgctc tctaaatttc acttcatttt cacaaaaaaa
    12901 cactcattat ttcaatgacc cacgtaatga tgcgccttaa ataatgagtg ctttattaat
    12961 atgttagcct aaacggtctt tcaaaaattc tatccatttc gttgtggcat gacttaattg
    13021 tttatccttc ttccaaacaa cacctaattc ccaatgtaca tgagcatctt caatgcgtag
    13081 cagcttcaca tctccattta gttgctctga aattgatgtt ggtaaaatac taatacctaa
    13141 ttcattcgta actaaatctt ctataacatg ccattgtgaa atttgcgcta cagtattcgg
    13201 aacaaagcca acgtttttag cattttcaat aattttatca ttcaagtaaa agtctttatt
    13261 aaataaaatg aagtcttcac ctgctaaatc ttcgagttta acagtttcat attttgcgag
    13321 tcgatgctct ctgctcacga taagtcgcaa atcttcctta tctagggtag tataatcgaa
    13381 aatatgatga tcgactggca aagtggtcac gcctatatct acttcatcat tgataatttg
    13441 ctgttcaatt gttttaccgc cattttctat caaattatat gtaacatttg gatattgttg
    13501 atggaatgca ccaagaatat tgataagaat cttcatattc atgactgccg acatgcccat
    13561 gtttatatgt cctgtttcca gtccattcaa gcgttccatt tcagatggta aataatcata
    13621 cagtgcaaca atttctttac ttttctcata aaaaatttga cctgcatcgg taagaattaa
    13681 atgtctttta cttctatcaa ataagggcgt gcccatttca ttttcaatat ctttaattgc
    13741 tttactaatt gtaggttgtg caatatataa tgatttggaa gcattagtca tgcctccttg
    13801 cttaacgact tcaataaaat atttcatatg tttgatatcc actaacatca tcctaactat
    13861 ttcaacacgt ttatttcatt atacacaact tcttaaacat tcaataaatt tagatttata
    13921 aaattgcttt ataaaaagtt ttatgtaaaa tacacattct tttatggtat tatattaatg
    13981 gctcaagtat attaatcgag gtgaggtatt gagcttttac gaatttatgc aaagttttct
    14041 tggtgatgac acaccattag gtgaattggt tgattggatt aatcaagata gcaatttccc
    14101 tagagaagtg aagagccata gtgaaatatt gtcatatttt cgaacaaatc catgtcctga
    14161 aagcatctca gtacctgtaa ttaaacgggc actatcagtt ttcaaccaat tcacaaatgt
    14221 acaatgatac atgaatacat ggagtcacat catctcatgt tagcgctctg attgcagggc
    14281 gctattttta atgttattgc tacatttagt atattgtgat tgctattttc tttcagttat
    14341 agaaccacgt accttttctt ttaaatcatt ttatattttc ccactaatat ccgctgttaa
    14401 tcaatcctga catccttgta tcactatgac aattaattgt taaatacatg aatttctaca
    14461 ttttatgaaa aaatccattt ttattacaat tcaacacttt atatgacaac ttcattacag
    14521 ttacttttat tgttgattgc ttacattgtt ttctaaaaaa aatttgttat cataattaac
    14581 gttgaataaa gaaaaaaatt aacttgggag ataaaaatgg aatataaaaa gatactaatt
    14641 cgtttattaa ttgcttttgc agtacttttc tcagcagatt tcacttatca atccgtcgaa
    14701 caaacgcatc aatcgcatgc cgcagttaat tattatagta aaaaccaatg tacatggtgg
    14761 gcatttaaac gtcgcgcaca agtcggtaaa cctgtttcta atagatgggg caatgctaaa
    14821 aattggtatt acaatgcacg taaatcaaaa tatgcgactg gtcgtacacc aagaaaattt
    14881 gctgtcatgc aatcaactgc aggatattat ggacatgtcg cagttgttga acaagtatat
    14941 aaaaacggta gtattaaagt ttcagaatac aacttttatc gcccattaaa atacaataca
    15001 cgtgtactaa gcaaaaaggc agcacgtaac tttaactata tttactaatc aaaaaacttc
    15061 tatcacgaac gcttcaattt cctgtatgcg tgtgatagaa gtttttattt ttgttcatat
    15121 taatttatct aagcgctacg atggactgac ctaaactttt ctttaaaatc gctatttgca
    15181 atttactatt gttggcttct taaatcttgt aaaatacgtt ccgctgcttg tgtatttgca
    15241 cggggttctt ttttcaaagc ttcagctact ttagcaattt catcaccttt tgctcctaca
    15301 acaatagcta atgatttata ttgtaaactc atatgacctt gttgaatacc ttctgacaca
    15361 agcgcgcgac atgctgcaaa gttttgcgct aaaccaacgg cagcaactac atgacctaat
    15421 tcttgtgctg actctacatt tagtagctct aatgaagctt tagctattgg taatactttc
    15481 gtaccacccc caacaattgc caatgtcata ggcacttcaa ttgtaccaat caatcgttga
    15541 cgatcttgat cgtaacgcca tgtagcaata ccacgatact gtccgtcacg acttgcgtat
    15601 gcatgcgcac ttgcttctgc accacgcgta tcatttcctg ttgctaaaac aacagcatgt
    15661 atgccattca taacaccttt attatgtgtt gctgcacgat gaatatctac ttgtgccaat
    15721 acagaagcac gttccattcg tttggcaacc tcttctccag ttctctcgcc ccttgctaaa
    15781 tctttaacat caatttcgcc ttgaacttta acaacggacg ctgttgcatg attggataaa
    15841 atactcatta aaatgtcgct ttgcggaaat tcatttttta aaaatgcagt tatggcctct
    15901 aaaatcgtat taagcatatt agcgcccata gcatctttcg tatcaacaaa tacttttaaa
    15961 gatagtaact gttgctcagg aaatgtatca atcgctatac gttggtaacc accaccacgc
    16021 gctttaatag aaggatatgc ctcatccgca attttatgaa tttgcttttc taaagcttta
    16081 atgtctgctg ataatttttc agtatcgtca acgccatcaa agacgatttg acctatcata
    16141 atacgttcag aagataccgt tttaaatccg ccagtctgat tcactagctt tgcaccataa
    16201 ctagctgcag cgacaactga aggctcttcc accatcatag gtacaacata tgccttatcg
    16261 tccacaatga tattcggtaa taatccaacg ggtaatgcac cttgcgcgat gacattttca
    16321 attaaactat tggctacttc ttcatcgatt aatggatgat tcagtaaaat gtcgaattgt
    16381 tcttctgata accattgctt atcaaccaat tgttgtaact tttctttacg agataaatgt
    16441 cgaaaattct tatctaaatt ttgcatggac gtactccttt tacttcacat aatttttaac
    16501 atttcaatca ctactatttt taccacaaaa taacgtcatt cgtcttaaaa ttcaattgaa
    16561 taattgtcgt tttgacttta aaataaaaca aggtaaatta aaacgcttac aagaaacgac
    16621 aaatcatttt taaatttagt atatttcttt gtataaaatt agcatattct gatatgatac
    16681 aagtgttgct tttataaatt tgaaaggatg taaaacctta tgacaatagg tatcgataaa
    16741 ataaactttt acgttccaaa gtactatgta gacatggcta aattagcaga agcacgccaa
    16801 gtagatccaa acaaattttt aattggtatt ggtcaaactg aaatggctgt tagtcctgta
    16861 aaccaagaca tcgtttcaat gggcgctaat gctgctaagg acattataac agacgaagac
    16921 aaaaagaaaa ttggtatggt aattgtggca actgaatcag cagttgatgc tgctaaagca
    16981 gccgctgttc aaattcacaa cttattaggt attcaacctt ttgcacgttg ctttgaaatg
    17041 aaagaagctt gttatgctgc aacaccagca attcaattag ctaaagatta tttagcaact
    17101 agaccgaatg aaaaagtatt agttattgct acagatacag cacgttatgg attgaattca
    17161 ggcggcgagc caacacaagg tgctggcgca gttgcgatgg ttattgcaca taatccaagc
    17221 attttggcat taaatgaaga tgctgttgct tacactgaag acgtttatga tttctggcgt
    17281 ccaactggac ataaatatcc attagttgat ggtgcattat ctaaagatgc ttatatccgc
    17341 tcattccaac aaagctggaa tgaatacgca aaacgtcaag gtaagtcgct agctgacttc
    17401 gcatctctat gcttccatgt tccatttaca aaaatgggta aaaaggcatt agagtcaatc
    17461 attgataacg ctgatgaaac aactcaagag cgtttacgtt caggatatga agatgctgta
    17521 gattataacc gttatgtcgg taatatttat actggatcat tatatttaag cctaatatca
    17581 ttacttgaaa atcgtgattt acaagctggt gaaacaatcg gtttattcag ttatggctca
    17641 ggttcagttg gtgaatttta tagtgcgaca ttagttgaag gctacaaaga tcatttagat
    17701 caagctgcac ataaagcatt attaaataac cgtactgaag tatctgttga tgcatatgaa
    17761 acattcttca aacgttttga tgacgttgaa tttgacgaag aacaagatgc tgttcatgaa
    17821 gatcgtcata ttttctactt atcaaatatt gaaaataacg ttcgcgaata tcacagacca
    17881 gagtaatctg tgtatttgaa agcatttaaa tataacctaa aaacagcagt aagacagctt
    17941 ctcattgaaa tcgtcttact gctgttctct atttataaca cttcgtattg aatgaattag
    18001 ctaagcctgt ttgacacatt attgaagttt tcctaatgcc tggatccttt atacgttacg
    18061 gcttcgtgct atgttttggt acataaagct ttgacatatc gatattctcc aactctaaca
    18121 gcttaatttt attattaatc gttccaccga accctgttaa gctacccgtt ttaccgacaa
    18181 cacgatgaca tggcacgata atagataatg gattacttcc gactgcacct ccaaccgctt
    18241 gggctgacat ttttggcttg ttaagcagct tgcctacttt tttggcaata gcaccatacg
    18301 ttgttagagt cccataagga acctgtctta attcattcca aacacactgt tgaaaatgac
    18361 tacctgttgg ctttaaaggt attgtgattt caggattgtc acctttaaaa tacgcgtcta
    18421 accactgtgt cgcctctcta aatatcgcta aagacgtatt ttcttcccta gtactatcac
    18481 cttgttgatt ttcaaacaaa acagcggtca gacttccccc atcactcaca agttccaatc
    18541 gtcctacagg cgaatcatag taactcttat actccataaa aatgccccct ttttctaatc
    18601 atacgcctta attgttaatt tagaaaatat tcatgttcac attttttaaa aataaataag
    18661 tcgtcaatta cgtatataaa cacgtaatac cagctatcac tttgctgcaa tatacagtta
    18721 catatcttac tacacgcgct aacctcttac tttgtaaacc aaatcttaaa ttaaaatatt
    18781 gaaaatgcaa tgaatcctta atattttatt aaacctataa ttacttatta aaaataacac
    18841 acaatattca taaagtttta aaaatattct gtttaatcat ctattattag tggaaaaata
    18901 taagagcaat tgtttatagt ttgcataacg cttcaaaagt attttctttt ttgtttagtt
    18961 caaaaaaatt tagaggtgat gttatatgaa taacggtttt ttcaatagcg actttgattc
    19021 aattttccga agaatgatgc aagatatgca aggttcaaat caagtcggta acaaaaagta
    19081 ctatattaat ggtaaagaag tttcacctga agaactagcg caactcacac aacaaggtgg
    19141 caatcactct gctgaacaaa gtgcgcaagc ttttcaacaa gcagcacaaa gacaacaagg
    19201 gcaacaaggt ggcaacggca attatttaga acaaattggt cgtaacctta cgcaagaagc
    19261 acgtgacggt ttattagatc cagtcattgg tcgtgataaa gaaattcaag aaactgctga
    19321 agtcttaagt agacgaacta aaaacaatcc tatattagtt ggagaagctg gtgttggtaa
    19381 aactgcgatt gttgaaggtt tagcacaggc aatcgttgaa ggaaatgtac cagcagcaat
    19441 caaagacaaa gaaattattt ctgtagatat ttcatcatta gaagctggaa cgcaatatcg
    19501 tggtgctttt gaagaaaata ttcaaaaatt aatcgaaggt gttaaatctt cacaaaatgc
    19561 cgtattattc tttgatgaaa tccatcaaat tatcggttca ggtgccacag gaagtgattc
    19621 aggtagcaaa gggttatctg atattttgaa acctgcatta agtcgtggtg aaatttcaat
    19681 tattggtgca acaacacaag atgaatatcg aaacaatatt cttaaagatg ctgcattaac
    19741 gcgcagattt aatgaagtac ttgttaatga accaagcgct aaagatactg ttgaaatttt
    19801 aaaaggtatt cgcgaaaaat tcgaagaaca ccatcaagta aaattaccag atgacgtatt
    19861 aaaagcatgt gttgacttat caattcaata tattccacaa cgattattac cagataaagc
    19921 aatcgatgtg ttagatatta cagcagcaca tttatctgcg caaagtccag ctgtcgataa
    19981 agttgaaact gaaaaacgaa tttctgaatt agaaaatgat aaacgtaaag cggtaagtgc
    20041 tgaagaatat aaaaaagctg acgacattca aaatgaaatc aaatcattac aagataaatt
    20101 agaaaatagt aatggtgaac atactgctgt tgctacagtt catgatattt cagatactat
    20161 tcaacgatta actggtattc cagtttctca aatggatgat aacgatattg aacgtttaaa
    20221 aaatatttct aatcgtttaa gaagtaaaat cataggtcaa gatcaagctg tagaaatggt
    20281 ttcacgtgca attcgccgta atcgtgctgg gtttgatgac ggcaaccgcc caattggcag
    20341 tttcctattt gttggcccta ctggtgttgg taaaacagag cttgctaaac aattagcaat
    20401 tgatctattt ggtaataaag atgcactgat tcgacttgat atgagtgaat atagtgacac
    20461 aacagctgtt tcaaaaatga ttggtacaac tgctggttat gtcggttatg atgacaattc
    20521 aaacacgtta actgaaaaag tacgccgtaa tccatactca gtcattctat ttgatgaaat
    20581 cgaaaaagca aatccacaaa ttttaacatt gttattacaa gtaatggatg atggtaattt
    20641 gactgatggt caaggtaatg tcatcaactt taaaaataca attattattt gtacatcaaa
    20701 tgctggcttt ggcaatggca atgacgctga agaaaaagat attatgcacg aaatgaaaaa
    20761 attcttccgc cctgaattcc ttaaccgctt caacggcatc gttgaattct tacatttaga
    20821 taaagatgca ttgcaagata tcgtcaactt attattagac gatgtacaag ttacattaga
    20881 taaaaaaggt attacgatgg acgtttctca agatgcgaaa gattggttaa ttgaagaagg
    20941 ctatgatgaa gaattaggtg cacgtccatt aagacgtatt gttgaacagc aagtacgtga
    21001 caaaattaca gattactatt tagatcatac agacgttaaa catgtggata tagatgttga
    21061 ggataacgaa ttagtcgtaa aaggtaaata acgacacttt aacatatcgc gcatcaaaaa
    21121 tgagcatcag gtcgcccttg cctgtgctca tttttttaat tatttccctg gaaaatgatt
    21181 cgctgtgtgc tgttctgttc cacaacaatc acgattaatg tcacatgtac cacattttcc
    21241 ttgtttcgaa cgtttgaaaa atttcactag tgtatacaag gcatatccga aaattgctaa
    21301 aaaaattaaa atgttaataa tgactgacac tttaaccact ccttaaacaa ataaatgtcc
    21361 gatttgataa aaaatgaatg ttaagacata tgcagtgact agaggataga caactgcaag
    21421 tgccgtccat ttccatgaat aagtctcttt acggattgct gccactgtag aaacacaagg
    21481 aatatacaat agtataaata tcataaatgc atacgcagat agcggtgtga attgattttg
    21541 aatcacatta acaaggcctg catcacctga tgaatagata atcgccatcg aacttacgat
    21601 aacttctttt gctaaaaatc ctggcactaa cgtagcacct gcttgccatg ttccaaatcc
    21661 gagcggttgc actaacatac caaagaaact accaaccata tgtaaaaaac tttgattgat
    21721 attcacattg ataccatgtg gtcctacata acttaatagc caaatgacta ctgagccgcc
    21781 aaaaataaat gtacctgctt tacgaacaaa gcccttagcc ttttcccaag tactacgcca
    21841 caacgtttta atggaaggca cacggtatgt tggcaattcc acaataaaga ttgcattatc
    21901 attttttaaa atcgtcttag taagtactgt actgactaaa aatgccataa taatacctaa
    21961 aacatacaag cttaatacta ctaaagattg attctctttg aaaaagatac ctacgaacaa
    22021 cgcatacact ggcagtctag cagagcatga catgaatggt gcaattaata tcgttgttaa
    22081 acgctctttt tcattttcaa tactgcgcgc agccataata ctcggtacgt tacaaccaaa
    22141 tccgataatc atcggtataa aagacttccc gcttaaaccg aacgattcca taatacgatc
    22201 cattaaaact gcaatacgcg ccatataacc tgaatcttct aataaagata taaagaaaaa
    22261 tagcacaaca atttgtggta caaagactaa tactgatcct acaccagcaa taatgccatc
    22321 tgtaattaaa tcttgtaaaa atggtataac gccaagataa ttcataatcg tcttcacact
    22381 atctgtaaat gtaccaccta taaatgcatc gagttgatcc gacaaaggtg tgccaatcca
    22441 tgtaaatgta gtttgaaaga tcaaccacat aatcgctaga aagataggca tccctatata
    22501 tttatgtgtt aatatcttgt ctattctaga gctgaaatat tgcttatctt catctggata
    22561 cgttaccacg tcttgcaata acgtctcaat ataatgattg cgtatacgct ccatctctcg
    22621 acgaacagat acagcctcta cttgtttatc aacttgatca cgtaaactcg acaatttatt
    22681 tacaacctct gaattaagtt cgtttgcaat ttgcatgtta tttaataaga attgaatcgc
    22741 aataaatcta gcttgatatt tatcatgaga tgtctctgtc attattattt gacacatatt
    22801 tttaattgtc tcttcaatct tctcaccata attgatttta aaatgcggtt gatacccttc
    22861 ccctagatgc tttatttcgc caagtaaatg tttagttcct ttgcctgtac gtgccacaac
    22921 tggaaaaata ggtgttttta actttttcat caatttatga taatcgattt ttatcccgcg
    22981 ctttgtagct acatcaatca tatttaatcc gatgtatatt ggttgattaa gttctaacaa
    23041 ttgtactgtt aattgcatat tcctttttag ttgactcgcg tcaacaatgt taatgattcc
    23101 tgaaaatgaa tcgtttaata aatagtctgt cactacagtt tcatctttag aaatcggcga
    23161 taaatcatat gtacctggta aatcaattaa ttgtcctaca ttttctttta gcttccctac
    23221 ttttttctct accgttacgc cactccagtt gcctatatat tcatacgaac cagttaaagc
    23281 gttaaacaaa gatgttttac caacattagg gtttcctaaa atacaataat tttccattcg
    23341 tccggctcct attcttctaa tgcaatagaa caagcatcgc aatgcctaat acttaactgt
    23401 tgtccgttta cttcaataat acatggccct ttaaataaac atttttgttt aatcgttatg
    23461 atagcgtcat ctgttaaccc aaaggcactt agacgataca acatattctc attagcaata
    23521 tccattcgct ttattttata agccttattc atttcaccat ttttaatgtt taacatacta
    23581 tttcgctctc ctattagaaa taataatcat tatcacttaa aaatcataac ccttaaaatt
    23641 gtagctcgca atactttatt taaataattt tcatttttca tgtaaaattt gtgacattgc
    23701 aaaaatgttt tgtataatca ctttcatgca aaaaacctcc gcttcaaatg tcattgatag
    23761 acatttaaag cggaggcatg tacatgttat tttatgattt atttcgtaga tttttttcta
    23821 acaattgatt gagcttttca atatttttat tcaactgttc tatattcgtt acttgttgtt
    23881 ttgaatttag ttgatgcttg ttttgataat ctattaaagc atcatataag aaaaatgaac
    23941 ttgctgattg ctgtttcact tcttggtaca atgccatagt tttatcatct agtactattg
    24001 aacgtacatc atcttgccca tgtacatctt gatgtttcga tacatttgtt gcaccttgtg
    24061 gatatatttt aaaggttgtg tcgtatgttt ccttactatc tttagcttca gattcctgtg
    24121 attcaaccgt tttatatttt tcaagtgcat gtccttcaat atcaactcgt ggaataatgc
    24181 gattcaacca tgctggtaaa taccacgaac cttttccaaa caatttcgtt aatgcaggaa
    24241 ttaacatcat tctgactacg aaggcatcaa agagtacacc aaacgctaat gccataccca
    24301 ttgatttaat catgacatct tcttggaata caaacgcaaa gaagacacta aacatgatta
    24361 atgcagctgc tacaataaca ggaccgcttt ctttcaaccc tactttgata gaataatcat
    24421 tatcccctgt tttactatat tcttcatgaa ttcgcgacat aaggaagact tcataatcca
    24481 tcgctaatcc aaataagata cctatagtaa taaccggtaa aaatgctagc attggtcctg
    24541 tcgtttcaat accaaacaga cctttcataa aaccatcttg cattactaat gttgtaaatc
    24601 ctagcgttgc cattaatgac aagacaaatc ctaaaactgc ttttaacggt attagaattg
    24661 aacggaagac aatcattaat aagaaaaatg ccaatacaac aattactgtt gcaaataaag
    24721 gtatcgcctc atttaacttt ttagacatat caatattaat gacactttgt cccgaaatct
    24781 ccgttttgaa cccatattta tcttgtgcat ctttatgata atctcgtaaa tcatgcacta
    24841 aatcatttgt actctctgca ttaggccctt tcttaggtat cacgaccatc aaagcgtaat
    24901 cattatcttt actcatttgt ggcggcgtaa cgatatctac atttttctta tctttaatat
    24961 ctttatatac agactgtaaa tcttgttgta atccttgtgg atcatccttt ttatctttca
    25021 catttatcaa catcggaatt tggccattaa atccttcacc aaatttatcc gagattatat
    25081 cgtaagcttt tttctgtgta gaatctgctg gtttaacacc gtcatctgga ataccaagtc
    25141 gcatatgact aactggtatt gcagctgcta ctaatatgat taaacctagt aatactgccg
    25201 caagtgcatt tcctgtaata aatttagacc aaggcgtatc aatatctttt ttaaaattag
    25261 actgcaattt gttcacttta atgcgtttat ggaaaatact aattaacgca ggtaataaag
    25321 tcagtgcact gaatacagca aatataacac taattgccga agcaaacccc atcactgcta
    25381 agaaatctat gccaactagc gataaaccgc atacggcaat cacaactgtc acaccagcga
    25441 agatgactgc gctacctgct gttcctattg caagaccaat gcctttaatg taatctgttt
    25501 cagttttcat aacttgtcga tatctgaata aaataaataa tgcataatcg ataccaactg
    25561 ctagtccaat cattacggct aatgtcagtg tgacatttgg tatatcgaat gcataagtta
    25621 acaaactgat aatgccaacc ccagatgcta gaccaatcaa tgcacttata attggtaatc
    25681 ctgcagcaat gactgaacca aatgtaatca ataacactac aaatgcaaca atgataccaa
    25741 ctagttcaga attaccgcct acttctgtag atgtcattcc tgtaccagtt aactctgttt
    25801 gtacattatg attgtcacgt aagtctttca aatgactttt aacattatct ctagagccat
    25861 cttttaaaga tgtttgacta acgtcatatg tgatatctgc aaatgcagtt gttttatctt
    25921 tactaatttg cttattttca taaggatctg atattttatc aatgtgcttg tcatcttttt
    25981 taatatcatc taacgttttc ttaatatctt tagtaatgtt cggttgcaca ataccatcat
    26041 ctttagtcgt cttaaagaca acacgtattt gtgccttttc actatcttga ttaaaatgtt
    26101 tttcaatctt tttattcgta tccaacgact ctaatcctgt cattttaata tcattgtcaa
    26161 atttcggtgc atttgtagca agtggtatca atattgcagc tacaatcact atccatgcaa
    26221 tgaccgcgga ccatttatgt tttgcgatga atgtccccat cttatataaa aattttgcca
    26281 aagtatattg cctcctttta aaatcaacgt tatagtttaa atatacagtg tagattattg
    26341 ttagattata gtatctatcc ccgacctctt aaagaatcaa ttggaaaatt ttgtatatta
    26401 aactacacac aaaggagaaa tgtagatgaa agagactgat ttacgagtta taaagacaaa
    26461 aaaagcattg tcgagtagct tgctacaatt gttagaacag caattattcc aaacgattac
    26521 tgtcaatcaa atttgcgaca acgcactcgt acaccgcaca acattttata aacattttta
    26581 tgataaatac gatcttctag agtatttgtt caatcaattg actaaagact attttgccag
    26641 agacattagc gatcgcctta accacccatt tcaaacgatt agcgatacaa ttaacaataa
    26701 agaagatttg cgagatattg cagaatttca agaagaagac gctgaattta ataaagtatt
    26761 aaaaaatgtc tgcattaaaa ttatgcataa cgatatcaaa aataatagag accgtatcga
    26821 tattgacagc gacatcccag ataatctcat attttatatt tatgactcgt tgattgaagg
    26881 ttttatgcat tggataaaag atgaaaaaat tgactggcct ggggaagaaa tt
//
LOCUS       DAMZKC010000020        26574 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.020, whole genome shotgun sequence.
ACCESSION   DAMZKC010000020 DAMZKC010000000
VERSION     DAMZKC010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 26574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26574
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.020"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            27..413
                     /locus_tag="POB55_000593"
     CDS             27..413
                     /locus_tag="POB55_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015978254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499579.1"
                     /translation="MMIKKLKNMDWFDIFIAGILRLFGVIALMLVVISPIYTVASYQN
                     KEVHQGTITDKYNKRQDKEDKFYIVLDNKQVIENSDLLFKKKFDSADIQARLKVGDKV
                     EVKTIGYRIHFLNLYPVLYEVKKVDK"
     gene            413..586
                     /locus_tag="POB55_000594"
     CDS             413..586
                     /locus_tag="POB55_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator RinB"
                     /protein_id="HDI6499580.1"
                     /translation="MIKQILRLLFLLAMYELGKYVTEQVYIMMTANDDVEAPSDFAKL
                     SDQSDLMRAEVSE"
     gene            587..988
                     /locus_tag="POB55_000595"
     CDS             587..988
                     /locus_tag="POB55_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499581.1"
                     /translation="MYSKESIVNMIGTHKMKCNVLADVIPEYDSNSIAQYGIQATLPK
                     PQGENSSKVEDVVVRLERANKRYAQMLKEVEFINQSQQRLGHVDFCFLELLKKGYNRD
                     AIIKKMPNSKLNRNNFLARRDELAEKIYLLQ"
     gene            1339..1833
                     /locus_tag="POB55_000596"
     CDS             1339..1833
                     /locus_tag="POB55_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HDI6499582.1"
                     /translation="MIKLTPKQEKFVLGLIEGKSQRKAYIDAGYSTKGKSGEYLDKEA
                     STLFKNRKVSGRYEKLRQEVAEQSKWTRQKAFEEYEWLKNVAKNDIEIEGVKKATADA
                     FLASLDGMNRMTLGNEVLANKKIETEIKMLEKKIEQIDKGDSGTEDKIKQLHDAITEV
                     IVNE"
     gene            1826..3049
                     /locus_tag="POB55_000597"
     CDS             1826..3049
                     /locus_tag="POB55_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HDI6499583.1"
                     /translation="MNKLKSLYTDKQIEILKQTQKQDWFMLINHGAKRTGKTILNNDL
                     FLRELMRVRKIADEEGIETPQYILAGATLGTIQKNVLIELTNKYGIEFNFDKYNSFML
                     FGVQVVQTGHNKVSGIGAIRGMTSFGAYINEASLAHEEVFDEIKSRCSGTGARILVDT
                     NPDHPEHWLLKDYIENTDPKAGILSYQFKLDDNNFLNDRYKESIKASTPSGMFYERNI
                     NGMWVSGDGVVYADFDLNENTIKADELNDIPIKEYFAGVDWGYEHYGSIVLIGRGIDG
                     NFYFIEEHAHQFKFIDDWVVIAKDIVCRYGNINFYCDTARPEYITEFRRHRLRAINAD
                     KSKLSGVEEVAKLFKQNKLLVLYDNMDRFKQEVFKYVWHPTNGEPIKEFDDVLDSLRY
                     AIYTHTKPERLRRGK"
     gene            3046..4470
                     /locus_tag="POB55_000598"
     CDS             3046..4470
                     /locus_tag="POB55_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016528746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDI6499584.1"
                     /translation="MTLYKLIDDIEAQGILPKHIEALIESHKDDRERMVNLYNRYKTH
                     IDYVPIFKRRPIEEKEDFETGGNVRRLDVSVNNKLNNSFDSEIVDTRVGYLHGVPVTY
                     DLDENAEKNEKLKKFITNFVIRNSVDDEDSEIGKMAAICGYGARLAYIDTNGDIRIKN
                     IDPYNVIFVGDNILEPTYSLRYFYEKDDDNGTDYVYAEFYDNTYYYVFRGEGIDALQE
                     VGRYEHLFDYNPLFGVPNNKEMIGDAEKVIHLIDAYDLTMSDASSEISQTRLAYLVLR
                     GMGMSEEMIQETQKSGAFELFDKDMDVKYLTKDVNDTMIENHLDRIEKNIMRFAKSVN
                     FNSDEFNGNVPIIGMKLKLMALENKCMTFERKMTAMLRYQFKVILSALKRKGYNLNDD
                     SYLNLIFKFTRNIPVNKLEESQVLINLKGQVSERTRLGQSQLVDDVDYELDEMEKESL
                     EFNDKLPDIYEGDANDKSQNNQSE"
     gene            4439..5389
                     /locus_tag="POB55_000599"
     CDS             4439..5389
                     /locus_tag="POB55_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HDI6499585.1"
                     /translation="MTNPKITNQNDIDEYIEGLISKAEKPIEQLFANRLKEIKQIIAD
                     MFEKYQSDDVYVTWTEFNKYNRLNKELTRIGTMLTDDYRQVAKMIQKSQEDAYIEKFL
                     MSLYLYEMASQTSMQFDVPSKEVIKSAIEQPIEFIRLMPTLQKHRDEVLKKIRMHITQ
                     GIMSGEGYSKIAKAIRDDIGMSKAQSLRVARTEAGRAMSQAGLDSAMVAKDNGLKMKK
                     RWHATKDTRTRDTHRHLDGESVEIDQNFQSSGCVGQAPKLFIGVNSAKENINCRCKLL
                     YYIDKDELPTVMRVRNDDGENEVIPFMNYREWEKHKRKKK"
     gene            5485..6069
                     /locus_tag="POB55_000600"
     CDS             5485..6069
                     /locus_tag="POB55_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HDI6499586.1"
                     /translation="MNIEEVKSFFEEHKDDKEVKDYLNGLKTVSVDDVKGFLDTEEGK
                     RFIQPELDRYHSKGLESWKEKNLENLIEQEVRKRNPEQSEEQKRISALEKELEKRDAE
                     AKREKLRSNALGKAQELNLPTSLVDRFLGDSDEDTEQNLKALKETFDKYVQEGVESKF
                     KSSGRDVKESQNQDLDPSNVKSIEEMAKEINIRK"
     gene            6086..7000
                     /locus_tag="POB55_000601"
     CDS             6086..7000
                     /locus_tag="POB55_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000235170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDI6499587.1"
                     /translation="MTTPTYTPGNVILSDFKNGVIPAEQGTLIMKDIMANSAIMKLAK
                     NEPMTAQKKKFTYLAKGVGAYWVSETERIQTSKPEYAQAEMEAKKIGVIIPLSKEFLK
                     WTAKDFFNEVKPLIAEAFYKAFDQAVIFGTKSPYNTSTSGKPLVEGAEEKGNVVTDTN
                     NLYVDLSALMATIEDEELDPNGVLTTRSFRSKMRNALDANDRPLFDANGNEIMGLPLS
                     YTGADVYDKKKSLALMGDWDYARYGILQGIEYAISEDATLTTLQASDASGQPVSLFER
                     DMFALRATMHIAYMNVKPEAFATLKPTE"
     gene            7012..7155
                     /locus_tag="POB55_000602"
     CDS             7012..7155
                     /locus_tag="POB55_000602"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499588.1"
                     /translation="MANPAEEIKVKKDNMTITVTKKVFDSYYSLVGYKEVKSRRTTSD
                     KSE"
     gene            7161..7511
                     /locus_tag="POB55_000603"
     CDS             7161..7511
                     /locus_tag="POB55_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016528745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HDI6499589.1"
                     /translation="MTLYEDVKLLLKKNGVEVKSDEEEIFKMEVDGILEDVRDITNND
                     FMKDGQVIYPYSIKKYVADVLEYYQRPEVKKNLKSRSMGTVSYTYNDGVPDYISGVLN
                     RYKRAKFHPFKPIR"
     gene            7523..7858
                     /locus_tag="POB55_000604"
     CDS             7523..7858
                     /locus_tag="POB55_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HDI6499590.1"
                     /translation="MFNPYDEFPHTISIGSIKKVGEYPIIQERFVSDKTINGFMDTPT
                     TSEQLKFHQMSQEYDRNLYVPYDLPISKNNLFEYEGRIFSIEGDSVDQGGQHEIKLLR
                     LKQVPYGKS"
     gene            7845..8258
                     /locus_tag="POB55_000605"
     CDS             7845..8258
                     /locus_tag="POB55_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002480803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDI6499591.1"
                     /translation="MAKVKYGADSMVVELDKFDKKIEEWVKKGIAKTTMKIYNTAVAL
                     APVDLGFLEESIDFKYFDGGLSSVISVGADYAIYVEYGTGIYATGPGGSRATKIPWSF
                     KGDDGEWYTTYGQAPQPFWNPAIDAGRKTFEQYFS"
     gene            8271..8696
                     /locus_tag="POB55_000606"
     CDS             8271..8696
                     /locus_tag="POB55_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000270192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HDI6499592.1"
                     /translation="MWVSVEPELTNQIYKRLISDPNINKLVDDRVFDVVQDDAVYPYI
                     VVGESNVTNNESSATMRETVGIVIHVYSQFATQYEAKLILSAIGYVLNRPIEIDNYEF
                     QFSRIDSQAVFPDIDRFTKHGTIRLLFKYRHKKKNEGVY"
     gene            8697..9254
                     /locus_tag="POB55_000607"
     CDS             8697..9254
                     /locus_tag="POB55_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDI6499593.1"
                     /translation="MAQKNYLAVVRPAETDLDPVESLLLADLQEGGHTIENDLAEIVR
                     GGKTDYSPNAMSESFKLTIGNVPGDKGIEAVKHAVQTGGQLRIWLYERNKRADGKHHG
                     MFGYVVPESFEMSFDDESDKIELSLKVKWNTAEGAEDNLPKEWFEAAGAPTVEYEKFG
                     EKVGTFENQKKASVVSDSHTEDHSM"
     gene            9321..9827
                     /locus_tag="POB55_000608"
     CDS             9321..9827
                     /locus_tag="POB55_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499594.1"
                     /translation="MTEFNPITTLKINDGEKDYEVEAKVTFAFDRKAEKFSEDSEDGR
                     KGAMPGFNVIFNGLLESRNKAILQFWECATAYLKNPPTREQLEKAIDDFITENEDTLP
                     LLQGALDKLNNSGFFKRESRSYWMTLNKAPNMAKSEDKEMTKAGIEMMKENYKEIMGA
                     EPYTITQK"
     gene            9872..10156
                     /locus_tag="POB55_000609"
     CDS             9872..10156
                     /locus_tag="POB55_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499595.1"
                     /translation="MLALTPAEWRDWLIGGQDRYLDQRQLLIEQAQANGLVQASKRLT
                     SMIRDIEKQRYEIREPGSYARVQKARLEEEKRRRELFKEGTRKFLESKGG"
     gene            10160..13045
                     /locus_tag="POB55_000610"
     CDS             10160..13045
                     /locus_tag="POB55_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HDI6499596.1"
                     /translation="MDTHFMAKIMANIRDFQSNVRKAQRLAKTSVPNEIETDVKADIS
                     RFQRALQRAKAMAQKWREHNVKIDGNNSPLKRAIASAKTMLATLHNKTIKVNFDTRGM
                     TKTQILTKALNQSLTDYSEKMDALATKIRTFGTIFAQQVKGLMIASIQALIPVIAGLV
                     PAIMAVLNAVGVLGGGVLGLVGAFSVAGLGAVGFGAMAISALKMVEDGTLAVTKEVQD
                     FRKASDQLKDTWRDIVKENQAKIFNAMSAGLRGITSALTKMKPFLSEVSMLVEANVRK
                     FEEWVKTSNTAKKAFESLNTIGGAIFGDLLNAAGRFGDGLVNIFTQLMPLFKFMSQGL
                     QNMSIDFQNWANSVAGQNAIQAFIEYTKTNLPKIGKIFGNVFKGIGNLMIAFSQNSSN
                     IFDWLVELTNKFREWSEQVGQSQGFKDFVNYVQENGPTIMNLIGNIVKALVAFGKAMA
                     PIASKLLNFITNLADFIGKLFETHPAVAQAMGILGILGGAFWALMAPIAAVSSVLSNV
                     FGTSLLRIIKKMLDLTGIPELLSKAFAPLAGVFTSISWPVLLVIAIIAAFIGVVVYLW
                     KTNETFRKNMIKAWEDIKTAVSEAIQGVIDWLSELWDKIQTTIEPIKPILEALGKIFM
                     TVLGVLVIGVIVNVMNVIQGLWTVIKIAFEAIGTVISVAVQIIVGLFTALIQFLTGDF
                     SGAWETIKNTVSNVLETIWEYMQSVWESIIGFLTGVMNRTLSMFGTSWSQIWSTITNF
                     VSSIWNTVTSWFSRVASSVAEKMGQALNFIITKGSEWVSNIWNTVTSFASKVADGFKR
                     VVSNVGDGMSDALGKIKSFFSDFLNAGAELIGKVAEGVANAAHKVVSAVGDAISSAWD
                     SVTSFVSGHGGGSSLGKGLAVSQAKVIATDFGSAFNKELSSTLTDSIVDPVSTSIDRH
                     MTGDVQHSLKENNRPIVNVTIRNEGDLDLIKSRIDDIDAIDGSFNLL"
     gene            13060..14001
                     /locus_tag="POB55_000611"
     CDS             13060..14001
                     /locus_tag="POB55_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HDI6499597.1"
                     /translation="MIAHDIEVIRNGSQYRVSDNPFTYNHLEVVEYNVTGAGYHRNYS
                     DIEGIDGRFHNFAKEELKKVELKLRYKVPKIAYASHLKSDVQTLFAGRFYLRELATPD
                     NSIKYEHILDIPKDKQAFELDYVDGRQLFVGLVSEVSFDTTQTSGEFSLSFETTELPY
                     FESVGYSTDLESDNDPEKWSAPDRLPTNEGDKRRQMTFYNTNSGEVYYNGDVPLTQFN
                     QFNVVEIELAEDVKANDKDGFTFYTDKGNISVIKEVDLKAGDKIIFDGKHTYRGYLNI
                     DSFNKTLEQPVLYPGWNRFKSNKVMKQITFRHKLYFR"
     gene            14012..15898
                     /locus_tag="POB55_000612"
     CDS             14012..15898
                     /locus_tag="POB55_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDI6499598.1"
                     /translation="MPILLKSLQGVGHAINVSTKVSKKLNEDSSLDLTIIENASTFDA
                     IGAITKMWTITHVEGEDDFNEYVIVILDKSTIGEKIKLDIKARQKELDDLNNSRIYQE
                     YNESFTGVEFFNTVFKGTSYKYVLHTKVDASKFEGLGKGDTRLEIFKKGLERYHLEYE
                     YDAKTKTFHLYDELSKVANYYIKSGVNADNVKIQEDASKCYTYIRGYGDFDGQQTFTE
                     AGLQFEFTHPLAQLIGKREAPPLIDGRIKKEDVLKKSMELVIKKSVTASISLDFVALR
                     EHFPEANPKIGDVVRVVDSAIGYNDLVRIVEITTHRDAYNNITKQDVVLGDFTRRNRY
                     NKAVHDAANYVKSVKSTKSDPSKELKALNAKVNASLSINNELVKQNEKINAKVDKMNT
                     KTVTTANGTIMYDFTSQSSIRNIKSIGTIGDSVARGSHAKTNFTEMLGKKLKAKTTNL
                     ARGGATMATVPIGKEAVENSIYRQAEQIRGDLIILQGTDDDWLHGYWAGVPIGTDKTD
                     TKTFYGAFCSAIEVIRKNNPDSKILVMTATRQCPMSGTTIRRKDTDKNKLGLTLEDYV
                     NAQILACSELDVPVFDAYHTDYFKPYNPAFRKASMEDGLHPNEKGHEVIMYELIKDYY
                     SFYD"
     gene            15911..17809
                     /locus_tag="POB55_000613"
     CDS             15911..17809
                     /locus_tag="POB55_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499599.1"
                     /translation="MAYGLITSLHSMTGRKIVAQHEYNYRLLDEGMSKLEKMFIYHQK
                     EEIYAHSAKQIKYLNDSVEDYLTYLNGRFSNMILGHNGDGINEVKDARIDNTGYGHKT
                     LQDRLYHDYSTLDAFTKKVEKAVDEHYKEYQATEYRFEPKEQEPEFITDLSPYTNAVM
                     QSFWVDPRTKIIYMTQARPGNHYMLSRLKPNGQFIDRLLVKNGGHGTHNAYRYIDGEL
                     WIYSAVLDSNKNNKFVRFQYRTGEITYGNEMQDVMPNIFNDRYTSAIYNPIENLMIFR
                     REYKASERQAKNSLNFIEVRSADDIDKGIDKVLYQMDIPMEYTSDTQPMQGITYDAGI
                     LYWYTGDSNTANPNYLQGFDIKTKELLFKRRIDIGGVNNNFKGDFQEAEGLDMYYDLE
                     TGRKALLIGVTIGPGNNRHHSIYSIGQRGVNQFLKNIAPQVSMTDSGGRVKPLPIQNP
                     AYLSDITEVGHYYIYTQDTQNALDFPLPKAFRDAGWFLDVLPGHYNGALRQVLTRNST
                     GRNMLKFERVIDIFNKKNNGAWNFCPQNAGYWEHIPKSITKLSDLKIVGLDFYITTEE
                     SKRFTDFPKDFKGIAGWILEVKSNTPGNTTQVLRRNNFPSAHQFLVRNFGTGGVGKWS
                     LFEGKVVE"
     gene            17809..19632
                     /locus_tag="POB55_000614"
     CDS             17809..19632
                     /locus_tag="POB55_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HDI6499600.1"
                     /translation="MIVDNFSKDDNLIELQTTSQYNPIIDTNISFYESDRGTGVLNFA
                     VTKNNKPLSISKHNAMTSIVLKTDNFDDEHGAYISDELTIVDAINGRMQYVIPNEFLK
                     YTGRVHAQAYFTQNGSNNVIVERQFNFNIQNDLISNFDGKTKLVYIKSIQDLTESVKE
                     EVEDLKKSLSDTKSLVTEIDSRINQGIQRLEIKQNEAVQMITTTQDKAVQYINSEFQK
                     IVDKEQAIFERVNEVEQQINGADLVKGNSTTNWQKSKLTDDYGKAIESSEQSIEAVLR
                     HANSSMIIHITNAKDAPEKADIGTLEKPGQDGVDDGSSFDESTYTSSKSGVLVVYVVD
                     NNTARATWYPDDSNDEYTKYKIYGTWYPFYKKNDGNLTKQFVEEISNNTLNQAKQYVD
                     GKLQSISWQQHKLTEHNGQSIQKNLYNAKGNLEALGAGNYYVTSVPDLPGIVESYEGY
                     LSVFVKDDANKLFNFTPLNSKKVYTRSITNGRLDSQWATPNEHKTAVLFDGAANGVGT
                     RINLTEAYTNYAILFISGTYPGGVIEAFSLTSIPNAIQLSKTNVVDSDGNGGGSYECL
                     ITKESGTTLKIDNDVYLDLGSKTGSGANANRVTINKIVGWK"
     gene            19632..20009
                     /locus_tag="POB55_000615"
     CDS             19632..20009
                     /locus_tag="POB55_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2977 domain-containing protein"
                     /protein_id="HDI6499601.1"
                     /translation="MKITVNDKNEVIGYVNTGGLRNSLDVDDNNVPIKFKEEFEPRKF
                     IFTNGEIKYNSNFEKEDVPNASNQQSESDLSDEELRGMVASMQMQVTQVNILAMELKQ
                     QNAMLTQQLTELKAGKTNTEEDV"
     gene            20019..20192
                     /locus_tag="POB55_000616"
     CDS             20019..20192
                     /locus_tag="POB55_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HDI6499602.1"
                     /translation="MKMIYPTFKDIKTFYVWGCYKNEQIKWYVDMGVIDKEEYALITG
                     EKYPEAKDEKSQV"
     gene            20233..20532
                     /locus_tag="POB55_000617"
     CDS             20233..20532
                     /locus_tag="POB55_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2951 domain-containing protein"
                     /protein_id="HDI6499603.1"
                     /translation="MFGFTKRHEHEWRIRRLEENDKTMLSTLNEIKLGQKTQEQVNIK
                     LDKTLDAIQRERQIDEKNKKENDKNIRDMKMWILGLIGTIFSTIVIALLRTIFGI"
     gene            20669..22543
                     /locus_tag="POB55_000618"
     CDS             20669..22543
                     /locus_tag="POB55_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016187665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDI6499604.1"
                     /translation="MGLPNPKTRKPTASEVVEWAKSNIGKRINIDNYRGSQCWDTPNF
                     IFKRYWGFVTWGNAKDMANYRYPKGFRFYRYSSGFVPEPGDIAVWHPGNGIGSDGHTA
                     IVVGPSNKSYFYSVDQNWVNSNSWTGSPGSLVRHPYVSVTGFVRPPYSKDTSKPSSTD
                     TSSASKANDSTITGEAKKPQFKEVKTVKYTAYSNVLDKEEHFIDHIVVMGDERSDIQG
                     LYIKESMHMRSVDELYTQRNKFISDYEIPHLYVDREATWLARPTNFDDPRHPNWLVIE
                     VCGGQTDSKRQFLMNQIQALIRGVWLLSGTDKELSETTLKVDPNIWRSMKDLINYDLI
                     KQGIPDDAKYEQVKKKMLETYIKRDILKRENIKEVTTQTTIRISDKTSVDSASTRGPT
                     ASDEKPSIVTEKSPFTFQQALDRQMSRGNPKKSHTWGWANATRAQTSSAMNVKRIWES
                     NTQCYQMLNLGKYQGVSVSALNKILKGKGTLDGQGKAFAEACKKNNINEIYLIAHAFL
                     ESGYGTSNFASGRYGAYNYFGIGAFDNDPDYAMKFAKNKGWTTPAKAIMGGASFVRKD
                     YINKGQNTLYRIRWNPKNPATHQYATAIEWCQHQASTIAKLYKQIGLKGIYFIRDKYK
                     "
     gene            22556..23794
                     /locus_tag="POB55_000619"
     CDS             22556..23794
                     /locus_tag="POB55_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HDI6499605.1"
                     /translation="MYKIKDVETRIKNDGVDLGDIGCRFYTEDENTASIRIGINDKQG
                     RIDLKAHGLTPRLHLFMEDGSIFKNEPLIIDDVVKGFLTYKISKKVIKHAGYVRCKLF
                     LEKEEGKIHVANFSFNIVDSGIESAVAKEIDVKLVDDAITRILKDNATDLLSKDFKEK
                     IDKDVISYIEKNESRFIGAKGDKGEPGQPGAKGEAGKKGEQGVPGKNGTVVSINPDTK
                     MWQIDGKDTDIKAEPELLDKINIANVEGLENKLQEVEKIKDTTLNDSKTYTDSKIAEL
                     VDSAPESMNTLRELAEAIQNNSISESVLQQIGSKVSTEDFERFKQSLNSLYADKNHSH
                     TIKQIEGLENALSKKSDINHSHDERYLLSSNAFTKEEADKLYQPIGSSQPSLNIWTGS
                     ETEYNYLYQKDPNTLYLIKG"
     gene            23799..24194
                     /locus_tag="POB55_000620"
     CDS             23799..24194
                     /locus_tag="POB55_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499606.1"
                     /translation="MEGNFKNVKKLIYEGEEYTKVYAGNIQVWKKPSSFVIKPLPKNK
                     YPDSIEESTAKWTINGVEPNKSYQVTIENVRSGIMRISQTNLGSSELGISGVNSGVAS
                     KNINFSNPSGTLYVTISDVYSGSPTLTIE"
     gene            24250..24525
                     /locus_tag="POB55_000621"
     CDS             24250..24525
                     /locus_tag="POB55_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HDI6499607.1"
                     /translation="MDIGTIVRTILLIVAWINQFLAIKHISPIPVDEVFISTVVTGIV
                     SIWTWWKNNNFTHASKKGQQKIYEVKAGIQSTGGAPKVNGDDNNAVG"
     gene            24512..25924
                     /locus_tag="POB55_000622"
     CDS             24512..25924
                     /locus_tag="POB55_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015978294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDI6499608.1"
                     /translation="MPSVRTYSQAISYLKSLEGKAWNPDNAFGCQCFDTANQYWLYLF
                     NHRLKGVGAADIPTWNDFTNEATVYENTVSFQALPGDVVIFNRNYGGGYGHVGIVISA
                     TLDSITILEQNWLGGAYWSPPEVTTRRTHGYDFPMWFIRPFYAKETTANKLRSAVTPV
                     KQDKLSKGKKIMLVAGHGIGAYSNDPGAVANGENERDFNRKNIIPRVKKYLESVGNTV
                     LLYGGNSMNQDLYQDTLYGQRVGNYKDYGMYWIKSEVKPDAIIEFHLDSASPQASGGH
                     VIISDRFPADDIDKALSSALDKTVGKIRGVTPRGDLLNANVSADLNLNYRLIELGFIT
                     STKDLNYIKNNLDSFTKRIAEAINGRQIDAPSSKPSADKITWNWKGVFYPNPEKAIRV
                     RKTAGLTGTVVEEDSWLYTKDDWVKFDQVIKKDGYWWIRFKYQREGSSTNNFYCAVCR
                     ITDKEQKIKNEKYWGTIEWA"
     gene            complement(25984..26541)
                     /locus_tag="POB55_000623"
     CDS             complement(25984..26541)
                     /locus_tag="POB55_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015978304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4888 domain-containing protein"
                     /protein_id="HDI6499609.1"
                     /translation="MNKKLLTKTLIASALVLTTVGSGFHSSSNYNGINNVAKASEITD
                     SELWKNVRDALKDANIIDKTNHETIKVKYKLKNGGESEISGTANLDNLSNTNNSTVSP
                     DSVNRVDITRVNPNGNTIEANDAWKKLVDKLKEKHIVKVGDKVTIHSKDPSDEKVYGK
                     AGDQDSNVKNRLISPKDITHITIER"
BASE COUNT         9602 a         4028 c         5518 g         7426 t
ORIGIN      
        1 tagtttaatt gatactggag atgacaatga tgattaaaaa acttaaaaat atggattggt
       61 ttgatatctt tattgctgga atactgcgat tattcggcgt aatcgcactg atgcttgttg
      121 tcatatctcc tatctataca gtggctagtt accaaaacaa agaagtacat caagggacaa
      181 ttacagataa atataacaag agacaagata aagaagacaa gttctatatt gtgttagaca
      241 acaagcaagt catcgaaaac tctgacttac tattcaaaaa gaaatttgat agcgcagaca
      301 tacaagctag gttaaaagta ggcgacaaag tagaagttaa aacgattggt tatagaatac
      361 actttttaaa tttatatccg gtcttatacg aagtaaagaa ggtagataaa taatgattaa
      421 acaaatatta agactattat tcttactagc gatgtatgag ttaggtaagt atgtaactga
      481 gcaagtatat attatgatga cggctaatga tgatgtagag gcgccgagtg acttcgcaaa
      541 gttgagcgat cagtctgatt tgatgagggc ggaggtgtca gagtagatgt atagcaaaga
      601 gtcaattgtt aatatgatag gcacacataa aatgaagtgt aatgtattag cagatgtaat
      661 accggaatat gatagcaatt caattgcaca gtatggcata caagcaacgt tgccgaaacc
      721 acaaggggaa aactcaagta aagttgaaga tgttgttgtg aggcttgaga gagcaaataa
      781 aaggtatgct cagatgttaa aagaagttga gtttataaat caatcacaac agagattagg
      841 acacgttgac ttttgtttct tagagttatt gaagaaaggt tataacaggg atgcgattat
      901 caagaagatg cctaactcta aattaaacag aaacaacttc ctagcacgcc gtgatgagtt
      961 agcagaaaag atttatctac tacagtgacg aaaatgacga aaatgacaga aatgacgaaa
     1021 atgacactat ttttaaactg tgaattaatt ttatataatt gatttgtaag aattatctta
     1081 agacgtgggg taatagccac attagatgtt ctcatcgatg tgattgagaa gtgacaaaca
     1141 tgtaaagttg atatgttacg ctattgatca cttactacct gcctatatgg tgggtagttt
     1201 aattcttgca ttttgagtca taactatttt cctcctttcg catttattga acgtagctcc
     1261 tgcacaagat gtaggggcat ttttgtattt aaataactag agtaattaac gtaaaggcgt
     1321 gtgatacagt gaaaacaatt gattaaatta acaccgaagc aagaaaagtt tgtgctagga
     1381 ctcatagagg gcaagagcca acggaaagca tatattgacg cagggtattc gactaaaggt
     1441 aagagtgggg aatatctaga taaagaagcg agtacacttt ttaaaaatcg gaaggtttcc
     1501 ggaaggtacg aaaaattgcg tcaagaagta gctgaacaat caaaatggac acgccaaaag
     1561 gcctttgaag aatatgagtg gctaaagaat gtagctaaga atgacattga aatagaggga
     1621 gtgaagaaag cgacagctga tgcattcctc gctagtttag atggtatgaa tagaatgacg
     1681 ttaggtaacg aagttttagc taacaagaaa atagaaactg aaattaagat gcttgagaag
     1741 aagattgaac aaatagataa aggtgacagt ggaacagaag ataaaatcaa acaacttcac
     1801 gacgcaataa cggaagtgat cgtcaatgaa taaacttaaa tctttatata cggacaaaca
     1861 aattgaaata ttgaaacaaa cgcaaaaaca agattggttt atgttaatta atcacggagc
     1921 taagcgtaca ggtaaaacaa tattaaacaa cgacttattt ttacgtgagt taatgcgtgt
     1981 gcgaaagata gcagacgaag aaggaattga gacacctcaa tatatacttg ctggtgcaac
     2041 attaggtacg attcaaaaaa acgtactaat agagttaact aacaaatatg gcattgagtt
     2101 taattttgat aaatacaatt cattcatgtt atttggcgtt caagtggttc agacaggtca
     2161 caataaagta agtggtatag gagctatacg tggtatgaca tcgtttggtg catatatcaa
     2221 cgaagcgtcg ttagcgcatg aagaagtgtt tgacgagatt aagtcacgtt gtagtggaac
     2281 tggtgcaaga atattggtag ataccaaccc tgaccatccc gaacattggt tgttgaaaga
     2341 ttacatcgaa aatacagatc ctaaagcagg tatactgagt taccaattta agctcgatga
     2401 caataacttt cttaacgata gatataaaga gtctattaag gcttcaacac catcaggtat
     2461 gttctacgaa cgcaatatca acggtatgtg ggtgtctggt gacggtgtag tatatgccga
     2521 ctttgatttg aatgagaata cgattaaagc agatgaattg aacgacatac ctatcaaaga
     2581 atactttgct ggtgtcgact ggggttacga gcattatgga tctattgtgt taataggacg
     2641 aggtatagat ggtaactttt attttattga ggagcacgca caccaattta agtttattga
     2701 tgattgggtg gttattgcaa aagatattgt atgtagatat ggcaatatta atttttactg
     2761 cgatactgca cgacctgaat acatcactga atttagaaga catagattac gtgcaattaa
     2821 cgctgataaa agtaaactat cgggtgtaga ggaagttgct aagttgttca aacaaaacaa
     2881 gttacttgtt ctttatgata atatggatag gtttaagcaa gaggtattta aatatgtttg
     2941 gcaccctaca aacggagagc ctataaaaga atttgatgac gtgttggact cgttaagata
     3001 tgccatatac acacatacta aacctgaacg attaaggagg gggaaatgac attgtataag
     3061 ttaatagatg atattgaagc acaaggaata ttgcctaagc atattgaggc tctaatagag
     3121 tcacataaag acgatagaga gagaatggtt aatctctata atagatacaa gacacatatt
     3181 gactatgtac caatattcaa acgtcgacca attgaagaaa aagaagattt tgaaactggt
     3241 ggaaatgtaa ggcgattaga cgtgtctgtt aataacaaac ttaacaactc ttttgacagc
     3301 gaaattgttg atacacgtgt tggttattta catggtgttc ctgttactta tgatttagat
     3361 gaaaacgcag aaaaaaacga aaagttgaaa aagtttataa ccaactttgt cattagaaat
     3421 agtgttgatg atgaggattc tgaaataggt aaaatggcag caatttgcgg atatggcgct
     3481 aggttagcat atattgatac gaatggtgat attaggatta agaatataga tccctataat
     3541 gttatttttg ttggcgacaa tattttagaa cctacatact cgttgcgcta cttttatgaa
     3601 aaagatgatg ataatggcac tgattatgtg tacgcagagt tttacgataa tacttattat
     3661 tatgtatttc gaggagaagg tattgacgct ttgcaagaag ttggacgata tgaacattta
     3721 tttgattaca atccattgtt tggtgtacct aacaacaaag agatgatagg agatgctgaa
     3781 aaggttattc acttaattga cgcatatgat ttaacaatga gcgatgcatc aagtgagatt
     3841 agtcagacac gtttagcata ccttgtgtta cgcggtatgg gtatgagcga agaaatgatt
     3901 caagaaacac aaaagagtgg cgcatttgag ttgttcgaca aagatatgga cgttaaatac
     3961 ttaacaaaag acgtaaatga cacaatgatt gagaaccatt tagatcgaat tgaaaagaat
     4021 atcatgcgtt ttgcaaagtc agtaaacttt aattctgacg agtttaatgg aaatgtacct
     4081 atcattggaa tgaaacttaa gcttatggct ttagagaaca agtgtatgac gtttgagcgt
     4141 aagatgacag ctatgttgag gtaccaattc aaagttattt tatctgcatt aaagcgtaaa
     4201 gggtacaact taaatgatga tagttattta aatctgatat ttaagttcac tcgaaacatt
     4261 ccagttaata agttagaaga atcacaagtg ctaatcaacc ttaaggggca agtttcagaa
     4321 cgaacaaggt tgggacaatc acaactagtt gatgatgttg attacgaatt agacgaaatg
     4381 gaaaaagaaa gtcttgaatt taatgacaaa ttacctgata tatatgaagg tgacgcaaat
     4441 gacaaatccc aaaataacca atcagaatga tattgatgag tatatcgagg gtttaatctc
     4501 taaagcagaa aaaccaatag aacaactatt tgctaatcga cttaaagaga tcaaacaaat
     4561 catcgcagat atgtttgaaa agtatcaaag tgatgatgtg tatgttacat ggactgaatt
     4621 caataaatac aacaggctca ataaggagtt aactcgtata ggtacaatgt tgactgatga
     4681 ctataggcaa gtagctaaga tgattcagaa gtcacaggaa gatgcttata tcgaaaagtt
     4741 ccttatgagc ctttatttat atgaaatggc gagtcaaaca tctatgcagt ttgatgttcc
     4801 gagtaaagag gtaatcaaat cagctattga acaacctatt gagttcattc gtttaatgcc
     4861 aacactacaa aaacatcgtg atgaagtatt gaaaaagata cgtatgcaca ttacacaagg
     4921 tattatgagt ggagagggtt actctaagat agctaaagca atacgtgatg atattggcat
     4981 gtctaaagct caatcattgc gtgtggctcg cacagaagca ggcagagcaa tgtcgcaagc
     5041 tgggcttgat agtgcaatgg ttgctaaaga taatgggttg aagatgaaga aacgttggca
     5101 tgctaccaaa gatacacgta cacgcgatac tcatcgccat ttagatggtg aatctgtaga
     5161 aatagaccaa aactttcaat caagtgggtg cgttggacaa gcaccaaagc tatttatcgg
     5221 tgtaaacagt gcgaaagaga atattaattg tcgttgtaaa ttactctatt acattgataa
     5281 agatgaatta cccactgtga tgagagtgcg taatgatgat ggtgaaaatg aagttatacc
     5341 attcatgaat tatcgtgagt gggaaaaaca caagaggaaa aagaaataat gtatctatcg
     5401 accttagcat gtcgttaaac tgctttttat tatgcacttt tcggactgtt agggtacgcg
     5461 aagggcaaaa aggagttttg atatatgaat atcgaagaag ttaagtcttt ttttgaagaa
     5521 cacaaagacg ataaagaagt aaaagattat ctaaacggac ttaagacggt gtctgttgat
     5581 gacgttaaag gctttttaga tacagaagaa ggtaaacgat tcattcaacc tgaattagat
     5641 cgttatcatt cgaaaggatt agaatcatgg aaagagaaaa atcttgagaa tctaatcgaa
     5701 caagaagtac ggaagcgtaa tcctgagcaa tcagaagaac aaaaacgtat tagtgctctt
     5761 gaaaaagagt tagaaaaacg cgacgcagag gcaaaacgtg agaagttaag aagtaacgcg
     5821 ctaggtaaag cgcaggaact aaatttacca acatccttag ttgatagatt tttaggcgat
     5881 tctgatgaag atactgaaca aaacttaaaa gctttaaaag aaacatttga caagtatgtt
     5941 caagaaggtg ttgagtctaa atttaaatcg agtggaagag atgttaaaga atcacaaaat
     6001 caagatttag acccttcaaa tgtaaagtcc attgaagaaa tggcgaaaga aatcaatatt
     6061 agaaaataaa gtgaggtaat aaaatatgac aactccaaca tacacgccag gcaatgttat
     6121 tttatcggat tttaaaaacg gcgttattcc agcagaacaa ggtactttaa tcatgaaaga
     6181 cattatggct aattcagcaa ttatgaaatt agctaaaaat gagccaatga cagcacaaaa
     6241 gaaaaaattt acttacttag ctaaaggtgt aggcgcctac tgggtatcag aaacggaacg
     6301 tattcaaact tctaagcctg aatatgcaca agcagaaatg gaagctaaga aaattggtgt
     6361 aattattccg ttatcaaaag agtttcttaa atggactgca aaagatttct ttaatgaggt
     6421 taaaccttta attgcagagg cgttttacaa agcgtttgac caagctgtta tctttggtac
     6481 taaatcacct tacaacactt caactagtgg taaaccgctt gttgaaggcg cagaagagaa
     6541 aggtaacgtt gttacagata ctaataattt atacgtagac ctttcggcat taatggctac
     6601 tattgaagat gaagagttag atccaaacgg agtattaact acacgttcat tcagaagtaa
     6661 aatgcgtaat gctttagatg ctaatgacag accattattt gatgctaacg ggaacgagat
     6721 tatgggatta ccactatctt atactggagc ggatgtatac gacaaaaaga aatcgttagc
     6781 actaatgggt gattgggatt acgcacgtta cggtatctta caaggtattg agtatgcaat
     6841 ttctgaagat gccacgttaa cgacgttaca agcatcagat gcttctggcc aaccagtatc
     6901 attatttgaa cgtgatatgt tcgctttacg tgcgacgatg catattgcat acatgaacgt
     6961 taaaccagaa gcgttcgcaa cgcttaaacc aactgaatag gaggagatat gatggctaat
     7021 cctgcagaag agattaaggt aaaaaaagac aatatgacta ttactgttac aaagaaggta
     7081 tttgactctt attacagtct tgtcggttac aaagaggtta aatcacgtcg tactacgtct
     7141 gataagagcg agtgataaaa atgactcttt atgaagatgt taaactttta ctcaagaaaa
     7201 atggagtgga agttaaaagt gatgaagaag aaatatttaa gatggaagtt gacggaatac
     7261 tagaagatgt tagggatata acaaacaatg attttatgaa agatggtcaa gtcatttatc
     7321 cttactcaat caaaaagtat gtcgcagatg tcctagagta ttatcaacga cctgaagtta
     7381 aaaagaattt aaagtcaaga agtatgggga cagtgtcgta cacttataac gatggtgtcc
     7441 ctgattacat tagtggagta ttaaacaggt ataaacgagc aaagtttcat ccgtttaaac
     7501 caataaggta gaggtgttgt ttgtgtttaa cccatacgac gaattccctc acactatttc
     7561 tattgggagt atcaaaaaag taggagagta tccgattata caagagcgct ttgtaagcga
     7621 taaaacaatt aatggtttta tggatacacc tactacatct gaacaattaa aatttcatca
     7681 aatgtcccaa gagtatgaca gaaacctata tgtaccttat gacttgccaa tatctaaaaa
     7741 caatttattt gagtatgagg gtagaatctt tagtattgaa ggtgattctg tagatcaggg
     7801 tggacaacat gaaattaagc tactacgact taagcaggtg ccatatggca aaagttaagt
     7861 acggtgctga tagcatggtt gttgaattgg ataagttcga taagaaaata gaagagtggg
     7921 ttaaaaaagg tatcgctaaa acaacgatga agatatataa cactgctgta gcattagctc
     7981 ctgttgactt aggtttttta gaagaaagta ttgactttaa atatttcgat ggtgggttat
     8041 ccagtgttat aagtgtcggc gcagattatg caatatacgt tgaatacggt actggtatat
     8101 atgctactgg tcctggtggt agtcgtgcta caaagattcc gtggagtttt aaaggtgatg
     8161 acggcgaatg gtacacaaca tatggtcaag cgccacagcc attttggaac cctgcaattg
     8221 acgcaggacg caagacattc gagcagtatt tttcatagag gtggttaaat atgtgggtat
     8281 cagttgagcc tgaacttaca aatcaaatat ataaaagatt aatctcagac cctaacatta
     8341 acaaactagt tgatgatagg gtctttgacg ttgttcaaga tgacgctgtt tacccatata
     8401 ttgttgtggg tgaatcaaac gtcactaaca acgaatctag cgcaacaatg agagaaacag
     8461 tcggtattgt catacatgtg tattcacagt tcgctacaca atacgaggct aagctcattt
     8521 taagcgcgat aggttatgtg cttaacagac ctatagaaat agataattac gagtttcaat
     8581 ttagccgtat cgatagtcaa gcagtattcc ctgatataga caggttcact aagcatggca
     8641 cgatacggct tttatttaag tacagacata aaaagaaaaa cgaaggagtg tattaaatgg
     8701 cgcaaaaaaa ctatttagca gttgtacgtc cagctgaaac tgacttagat ccagtagaat
     8761 ctttattatt agctgactta caagaaggtg gacatacgat tgaaaatgat ttagctgaaa
     8821 tagtacgagg cggtaaaacg gactattctc ccaatgcaat gtcagaatca tttaaattaa
     8881 caattggtaa tgtgcctgga gataaaggaa ttgaagcagt gaaacacgct gtacaaacag
     8941 gtggacagtt gcgtatatgg ctttatgagc gtaataaacg tgcagacggt aaacatcatg
     9001 gaatgtttgg ttatgttgtt ccagaatcat ttgaaatgtc gtttgatgat gaaagtgaca
     9061 aaatcgaact atcattaaag gttaaatgga atacagcaga aggtgctgaa gataacttgc
     9121 cgaaagagtg gtttgaagct gcaggtgcgc ctacagttga atacgaaaaa ttcggcgaaa
     9181 aagtcggaac attcgagaat caaaagaaag ctagtgttgt atctgattca cacacggaag
     9241 accattctat gtaaacgaat agatcaaggg ggcgtaagct ccctattttt ttataaaaaa
     9301 attgaaaaga ggtatatatt ttgactgaat ttaatccaat tacaacatta aaaattaatg
     9361 acggagaaaa agattacgaa gtagaagcaa aagtaacatt tgcatttgac cgaaaagctg
     9421 aaaaattctc agaagatagc gaagatggga gaaaaggagc aatgccagga ttcaatgtta
     9481 tctttaacgg tttgctagaa tctagaaaca aagcgatttt acaattttgg gaatgtgcta
     9541 ctgcttattt aaaaaacccg ccaactcgag aacaattaga aaaagcaatt gatgatttca
     9601 tcactgaaaa cgaggatact ttgccgttat tacaaggggc tttggacaaa cttaacaata
     9661 gtggtttttt caagagggag agtcgctcgt actggatgac attgaacaaa gcaccgaata
     9721 tggccaaaag cgaggacaaa gaaatgacga aagcaggcat agaaatgatg aaagagaatt
     9781 acaaggaaat catgggcgca gaaccttaca cgattactca aaaataaggc aactgacagc
     9841 tagatattta ggatatatcc ctgaacatga attgttagca ctaacacctg ctgaatggcg
     9901 tgattggctt attggtggtc aggataggta cctagatcaa agacaattat taattgaaca
     9961 agcgcaagct aacggcttag tacaagcttc taagaggcta actagtatga ttcgtgacat
    10021 tgagaaacaa cgttacgaaa taagagaacc tggtagctat gctcgtgtac aaaaagctag
    10081 attagaagaa gaaaaaagaa gacgtgaact cttcaaagaa ggtacaagaa aattccttga
    10141 atcgaaagga ggttagcctt tggatactca ttttatggca aagattatgg ccaatattag
    10201 agatttccaa agcaacgtaa ggaaagctca acgattagca aagacgtctg taccaaacga
    10261 aattgaaaca gatgtaaaag cagatatttc aagattccaa agagctttac aacgcgctaa
    10321 agctatggcg caaaaatggc gtgaacataa cgttaaaata gatggtaata attcaccgtt
    10381 aaaacgtgca attgctagtg caaaaacgat gttggccacg ttacacaaca aaacaataaa
    10441 agttaatttc gatacgagag gtatgacaaa aacccaaatt ttaactaagg cactgaatca
    10501 gtccttaact gattatagtg agaaaatgga cgcgctagct actaaaattc gtacatttgg
    10561 tacaattttt gcacaacaag ttaaaggctt aatgattgct agtatacaag cattgatacc
    10621 agtgattgcc ggattagtac ctgcaataat ggcagtactt aatgcggttg gtgtattagg
    10681 tggtggcgtt ttaggtttag ttggcgcatt ctctgtcgca ggtcttggag ctgttggctt
    10741 tggtgcaatg gctattagcg ctcttaaaat ggttgaagat ggtacactag cagtaacaaa
    10801 agaggtacaa gacttcagaa aagccagcga tcaattaaaa gatacatggc gtgatattgt
    10861 taaagagaat caagctaaaa tatttaacgc tatgtcagca ggattaagag gtattacaag
    10921 cgcgttaact aaaatgaaac ctttcttgtc tgaggtatca atgttagttg aagcgaatgt
    10981 gcgtaagttt gaagaatggg tcaaaacgtc taacactgca aaaaaagcgt tcgagtcgtt
    11041 gaataccatc ggtggggcta tatttggtga tttattgaac gctgcaggac gatttggtga
    11101 tgggttagtc aatatcttca ctcaattgat gccgttattt aaattcatgt cacaaggtct
    11161 gcaaaacatg tctatagatt tccaaaattg ggctaatagt gtagctggac aaaatgcgat
    11221 acaagcgttt atcgagtaca ctaaaacaaa tctacccaag ataggcaaaa tattcggaaa
    11281 cgtttttaaa ggtattggta atttaatgat tgcgttcagt caaaacagtt ctaatatttt
    11341 tgattggctc gttgaattga cgaataagtt tagggaatgg tctgaacaag tagggcaatc
    11401 tcaaggtttt aaagatttcg tcaactatgt tcaagagaat ggcccaacta ttatgaactt
    11461 aatcggcaac attgttaaag cgttagtagc gtttggtaaa gcaatggcac ctatagctag
    11521 taaattacta aatttcatca ccaatctagc tgattttatc ggtaagttat tcgaaactca
    11581 tccggcagta gctcaggcta tgggtatttt gggcatatta ggtggtgcat tctgggcttt
    11641 gatggctccg attgcagcag tgagtagtgt tttaagcaat gtatttggta caagtttatt
    11701 aagaattata aagaaaatgt tagatttaac aggaataccg gaattattgt ctaaagcttt
    11761 tgcgccatta gctggtgtat ttacaagtat ttcttggcct gtattactag ttattgcaat
    11821 tatagctgcg ttcatcggtg ttgtcgttta cttatggaaa actaacgaga ctttcagaaa
    11881 gaatatgata aaagcgtggg aagatatcaa aaccgctgtt tctgaagcga ttcaaggcgt
    11941 aattgattgg ttaagtgaac tatgggataa aatacagacc acaatagaac caatcaaacc
    12001 gatattagaa gcgttaggta aaatttttat gactgtgtta ggcgttttag tgataggtgt
    12061 catagtaaac gtaatgaatg tcattcaagg attatggaca gtaataaaaa tagcattcga
    12121 agctatagga acagtgatat ctgtagcagt ccaaatcata gtaggtttat tcacggcgtt
    12181 aatacagttc cttacaggtg acttctcagg tgcttgggag acgattaaaa acacagtttc
    12241 caatgtgctt gaaaccatct gggaatatat gcaatcagtt tgggagtcaa ttatcggctt
    12301 tttaactggc gtaatgaatc gaacactttc tatgtttggt acaagttggt cacagatatg
    12361 gagtacaatc actaattttg ttagcagtat ttggaacact gttacaagtt ggttcagtcg
    12421 tgttgcttcg agtgtagctg aaaaaatggg acaagcacta aattttatta tcacaaaagg
    12481 ttctgaatgg gtttctaaca tttggaatac agttacaagt ttcgcgagta aagtagctga
    12541 tgggtttaaa agagttgtct caaatgtagg tgacggtatg agtgatgcac ttggtaagat
    12601 taaaagtttc ttcagtgatt tcttaaatgc cggagcggaa ttaatcggca aagtagctga
    12661 gggtgtagct aatgctgcgc acaaagtagt cagcgcggta ggcgatgcga tttcatcagc
    12721 ttgggactct gtaacttcat tcgtaagtgg gcacggtgga ggtagtagct taggtaaagg
    12781 tttagcggta tcacaagcaa aagtaattgc tacagacttt ggcagtgcct ttaataaaga
    12841 gctatcctct actttgacag atagtatagt agatcctgta agtacttcta tagacagaca
    12901 catgactggc gatgttcaac atagcttaaa agaaaataat agacctattg tgaatgtaac
    12961 tattagaaat gagggagacc ttgatttaat caaatcacgc attgatgata tagacgctat
    13021 agacggaagt ttcaacttat tataagggag gtttgttagt tgatagcgca cgatatagaa
    13081 gtaataagga atggttcgca atatcgcgtc agtgacaatc ctttcactta taatcacttg
    13141 gaagtagttg aatataacgt tacaggcgca ggctatcatc gtaactattc tgacatagaa
    13201 ggtatcgatg gtagatttca taattttgct aaagaagaac ttaaaaaagt agagcttaag
    13261 ttaaggtaca aagtacctaa gattgcttat gcttcacatt taaaatcaga cgtccaaaca
    13321 ctatttgctg ggcgttttta tttaagggaa ttagcaacac cagacaattc aattaagtat
    13381 gagcatatat tagatatacc aaaagacaaa caagcatttg agcttgatta tgttgatgga
    13441 cgacaacttt ttgtaggact agtaagtgaa gtttcttttg acacaactca aacatcaggg
    13501 gaattttctt tgtcgtttga aacaaccgaa ctaccatact ttgaaagtgt cggttatagt
    13561 actgatcttg aaagtgataa cgaccctgaa aaatggtcgg cacctgatag attgcctaca
    13621 aacgaaggtg ataagaggcg tcaaatgaca ttttacaaca ctaactcagg agaagtttat
    13681 tataacggtg atgttccttt aacacagttt aatcagttta atgttgttga aatagagtta
    13741 gctgaagatg ttaaagctaa tgataaggat ggattcactt tctatacaga taaaggaaat
    13801 atctcagtta ttaaagaagt tgatttaaaa gccggagata aaataatctt cgacggtaaa
    13861 catacctata gaggttattt aaatatagat tcttttaata aaactttaga acaaccggtt
    13921 ttatatccag gttggaatcg attcaagtct aataaagtaa tgaaacaaat tacatttaga
    13981 cacaaattat attttagata aggagtagcc tatgccaatt ttattaaaaa gtctacaggg
    14041 tgtagggcac gctattaatg ttagtacaaa agtaagtaaa aagctaaatg aagatagttc
    14101 tttggatcta actattatcg agaacgcgag tacgtttgac gcaataggtg ctataactaa
    14161 aatgtggacg atcactcatg ttgaaggtga agatgatttc aacgaatatg taattgtcat
    14221 acttgataag tctactattg gcgaaaaaat aaagcttgat atcaaagcta ggcaaaaaga
    14281 acttgatgac cttaacaatt ctaggattta ccaagagtat aacgaaagtt tcacaggcgt
    14341 agagtttttt aacactgtat ttaaaggaac tagttataag tacgtattgc acactaaggt
    14401 tgacgcatca aagttcgagg gattaggtaa aggagacaca agacttgaga tattcaaaaa
    14461 aggacttgaa cgctatcatc tcgaatatga gtacgacgct aaaacaaaaa catttcactt
    14521 gtatgacgaa ttatctaaag tagcaaatta ctatattaaa tcaggtgtaa atgctgataa
    14581 cgtcaaaatt caagaagatg cttctaaatg ctacacatat ataagaggtt atggcgactt
    14641 tgacggtcag caaactttta cagaggctgg gttacaattc gaattcacac acccattagc
    14701 acaactgatt gggaaaaggg aagcgcctcc gttaatagat ggacgcataa aaaaagaaga
    14761 tgttttgaaa aaatcaatgg agctagtgat aaagaaaagt gtcactgctt ctatttcctt
    14821 agactttgta gcgttacgtg aacatttccc agaagctaac cctaaaatag gtgatgttgt
    14881 tagagtggtg gattctgcca taggatataa cgacttagtg agaatagtcg aaatcactac
    14941 acatagagat gcgtacaata atatcactaa gcaagatgta gtattaggag actttacaag
    15001 gcgtaatcgt tataacaaag cagttcatga tgctgcaaat tatgttaaaa gcgtaaaatc
    15061 tacaaaatcc gacccatcta aagaactaaa agcattaaac gcaaaagtta acgcaagttt
    15121 atctataaat aatgaattgg ttaagcagaa tgaaaaaata aacgctaaag tcgataagat
    15181 gaatactaaa acagttacaa ctgctaatgg tacgatcatg tacgacttta ctagtcaatc
    15241 aagtataaga aacatcaaat caattggaac gattggcgac tctgtagcta gagggtcgca
    15301 cgcaaaaact aatttcacag aaatgttagg caagaaattg aaagctaaaa cgactaatct
    15361 tgcaagaggt ggcgcaacaa tggcaacagt tccaataggt aaagaagcgg tagaaaacag
    15421 catttataga caagcagagc aaataagagg agacctaatc atattacaag gcactgatga
    15481 tgactggtta cacggttatt gggcaggcgt accgataggc actgataaaa cggatacaaa
    15541 aacgttttac ggtgcctttt gttctgcaat tgaagttatt agaaagaata atccagattc
    15601 aaaaatacta gtgatgacag ctacaagaca atgccctatg agtggtacaa caatacgccg
    15661 taaagacacg gacaaaaaca aactagggtt aacacttgag gactatgtaa acgctcaaat
    15721 attagcttgt agtgagttag atgtaccagt gtttgacgca tatcacacag attactttaa
    15781 gccatacaat ccagctttta ggaaagcgag catggaggac ggcttacacc ctaacgaaaa
    15841 aggtcacgag gttattatgt acgagttaat caaggattat tacagttttt acgactaaag
    15901 gaggcaacca atggcttacg gattaattac aagtttacat tcaatgacag gtcggaaaat
    15961 agttgctcaa cacgagtata actatcgctt gttagatgaa ggtatgagca aacttgagaa
    16021 aatgtttata taccatcaaa aagaagaaat atacgcacac tcagcgaaac aaattaaata
    16081 cttgaatgac agtgttgaag attatttaac gtatttaaat ggccgtttta gcaatatgat
    16141 tctaggccat aacggcgacg gtatcaatga agtaaaagac gcgcgtattg ataatacagg
    16201 ttatggtcat aagacattgc aagatcgttt gtatcatgat tattcaacac tagatgcttt
    16261 cactaaaaag gttgagaaag ctgtagatga acactataaa gaatatcaag cgacagaata
    16321 ccgattcgaa ccaaaagagc aagaaccgga atttatcact gatttatcgc catatacaaa
    16381 tgcagtaatg caatcatttt gggtagaccc tagaacgaaa attatttata tgacgcaagc
    16441 tcgtccaggt aatcattaca tgttatctag attgaagccc aacggacaat ttattgatag
    16501 attgcttgtt aaaaacggcg gtcacggtac acacaatgcg tatagataca ttgatggaga
    16561 attatggatt tattcagctg tattggacag taacaaaaac aacaagtttg tacgtttcca
    16621 atatagaact ggagaaataa cttatggtaa tgaaatgcaa gatgtcatgc cgaatatatt
    16681 taacgacaga tatacgtcag cgatttataa tcctatagaa aatttaatga ttttcagacg
    16741 tgaatataaa gcttctgaaa gacaagctaa gaattcattg aatttcattg aagtaagaag
    16801 tgctgacgat attgataaag gtatagacaa agtattgtat caaatggata tacctatgga
    16861 atacacttca gatacacaac ctatgcaagg tatcacttat gatgcaggta tcttatattg
    16921 gtatacaggt gattcgaata cagccaaccc taactactta caaggtttcg atataaaaac
    16981 aaaagaattg ttatttaaac gacgtatcga tattggcggt gtgaataata actttaaagg
    17041 agacttccaa gaagctgagg gtctagatat gtattacgat ctagaaacag gacgtaaagc
    17101 acttttaata ggggtaacta ttggacctgg taataacaga catcactcaa tttattctat
    17161 cggccaaaga ggtgttaacc aattcttaaa aaacattgca cctcaagtat cgatgactga
    17221 ttcaggtgga cgtgttaaac cgttaccaat acagaaccca gcatatctaa gtgatattac
    17281 ggaagttggt cattactata tctatacgca agacacacaa aatgcattag atttcccgtt
    17341 accgaaagcg tttagagatg cagggtggtt cttggatgta ctgcctggac actataatgg
    17401 tgctctaaga caagtactta ccagaaacag cacaggtaga aatatgctta aattcgaacg
    17461 tgtcattgac attttcaata agaaaaacaa cggagcatgg aatttctgtc cgcaaaacgc
    17521 cggttattgg gaacatatcc ctaagagtat tacaaaatta tcagatttaa aaatcgttgg
    17581 tttagatttc tatatcacta ctgaagaatc aaaacgattt actgattttc ctaaagactt
    17641 taaaggtatt gcaggttgga tattagaagt aaaatcgaat acaccaggta acacaacaca
    17701 agtattaaga cgtaataact tcccgtctgc acatcaattt ttagttagaa actttggtac
    17761 tggtggcgtt ggtaaatgga gtttattcga aggaaaggtg gttgaataat gatagtagat
    17821 aatttttcga aagacgataa cttaatcgag ttacaaacaa catcacaata taatccaatt
    17881 attgacacaa acatcagttt ctatgaatca gatagaggaa ctggtgtttt aaattttgca
    17941 gtaactaaga ataataagcc gttatcaatc agcaaacata atgcgatgac tagtattgtg
    18001 cttaagacgg ataacttcga cgatgaacac ggcgcttata ttagtgatga acttacaatt
    18061 gttgatgcaa ttaacggacg aatgcaatac gttatcccaa atgagttctt aaaatacact
    18121 ggacgcgtac atgcgcaagc atattttact caaaacggta gcaataacgt aattgtagag
    18181 cgtcaattta acttcaatat ccagaatgat ctaattagta attttgacgg taaaacaaag
    18241 ctagtttata tcaaatcaat tcaggactta acagaaagtg ttaaagaaga agttgaggac
    18301 ttaaagaaaa gtttgagtga tacaaaatcg ttggttactg aaattgatag tcgtattaat
    18361 caaggtattc aaagattaga aatcaaacaa aatgaagcgg tacagatgat tacaacaaca
    18421 caagacaaag ccgttcaata tataaatagc gagttccaga aaattgttga taaagagcaa
    18481 gcgatttttg aacgtgttaa cgaagttgaa caacaaatca atggcgctga ccttgttaaa
    18541 ggtaattcaa caacaaattg gcaaaagtct aaacttactg atgattacgg taaagcaatc
    18601 gaatcatctg aacagtcaat agaagctgtt ttaagacacg ctaactcatc tatgattatt
    18661 catattacta atgcaaaaga tgcgccagaa aaggcggata taggcacgtt agagaagccc
    18721 ggacaagatg gtgttgatga cggttcttcg ttcgatgaat caacttatac atcaagcaaa
    18781 tctggtgtgt tagttgttta tgttgttgat aataatactg ctcgtgcaac atggtaccca
    18841 gatgattcaa acgatgagta cacaaaatac aaaatctacg gcacgtggta cccgttttat
    18901 aaaaagaatg atggaaactt aactaagcaa tttgttgaag aaatatctaa caacacactg
    18961 aatcaagcta aacagtatgt agatggtaag ttacaaagta taagttggca acaacataag
    19021 ttaacagaac ataacggtca atcaatccaa aagaacttat ataacgccaa aggtaattta
    19081 gaagcattgg gcgctgggaa ttattacgta acaagtgtgc ctgatttacc aggtattgtt
    19141 gaaagttacg aaggctactt atcagtattt gttaaagatg atgcaaataa gttattcaac
    19201 ttcacacctt taaactctaa aaaagtttat acacgatcaa tcaccaatgg tcgattagat
    19261 tcacaatggg ctacacctaa cgaacataaa acagccgtgt tattcgacgg tgctgcaaac
    19321 ggtgtaggaa caaggattaa tttaaccgaa gcttatacaa attatgcaat tctattcata
    19381 agcggtactt atccaggtgg tgttattgaa gcattcagtt taacctctat accaaatgcg
    19441 attcaattaa gtaaaacaaa tgtagttgac tcagacggta acggtggtgg tagttatgaa
    19501 tgcttaataa ctaaagaaag tggtacgacg ttaaaaatcg ataacgatgt gtaccttgat
    19561 ttaggcagta aaacaggttc tggtgctaat gcaaacagag ttacgataaa taaaattgtg
    19621 gggtggaaat aatgaaaatc acagtaaacg ataaaaacga agttatcgga tacgttaata
    19681 ctggcggttt acgcaatagt ttagatgtag acgataacaa tgtgcctatc aaattcaaag
    19741 aagagttcga acctagaaag ttcattttca ctaacggcga aattaaatac aatagcaatt
    19801 tcgaaaaaga agacgtaccg aatgcatcaa accaacaaag tgaatcagat ttgagtgatg
    19861 aagaacttcg cggaatggtt gcaagtatgc aaatgcaggt gacgcaagta aacattttgg
    19921 cgatggaatt aaagcaacaa aacgctatgt taacacaaca gttgactgaa ctaaaagctg
    19981 gtaaaacaaa tacagaggag gacgtttaaa tggagaaaat taagatgatt tatccaactt
    20041 tcaaggacat taaaactttt tatgtgtggg gttgctataa aaatgagcaa attaagtggt
    20101 acgtagacat gggtgtaatc gacaaagaag aatatgcatt gatcactggt gaaaaatatc
    20161 cagaggcaaa agatgaaaag tcacaggtgt aatgcttgag gctttttaat ttaacacaaa
    20221 gtaggtggcg taatgtttgg atttaccaaa cggcacgaac atgaatggcg aattagaaga
    20281 ttagaagaga atgataaaac aatgcttagc actctcaatg agattaaatt aggtcaaaaa
    20341 actcaagagc aagttaacat taaattagat aaaactttag atgctatcca gagggaaaga
    20401 cagatagacg aaaaaaataa gaaagaaaac gacaaaaata tacgcgatat gaaaatgtgg
    20461 attctcggtt tgatagggac tatcttcagt acgattgtca tagctttact aagaactatt
    20521 tttggtattt aaaggaggtg attaccatgc ttaaagggat tttaggatat agcttctggg
    20581 cgtgcttctg gtttggtaaa tgtaaataac agttaagagt cagtgcttcg gcactggctt
    20641 tttattttga ttgaaatgag gtgcatacat gggattacct aacccaaaga ctagaaagcc
    20701 tacagctagt gaagtggtgg agtgggcaaa gtcgaatatt ggtaagagga ttaatataga
    20761 taattatcgg ggcagtcaat gttgggatac acctaacttt atttttaaaa gatattgggg
    20821 ttttgtaaca tggggcaatg ctaaggatat ggctaattac agatatccta agggtttccg
    20881 attctatcgt tattcatctg gatttgtacc ggaacctgga gacatcgcag tttggcaccc
    20941 tggcaacgga ataggttcgg acggacacac cgcaatagta gtaggaccat ctaataaaag
    21001 ttatttttat agcgttgacc aaaactgggt taattctaat agttggacag gttctccggg
    21061 aagtttagta agacaccctt atgtaagtgt tacaggcttt gtcagacctc catattcaaa
    21121 agatactagc aaacctagta gtactgatac aagttcagca tcaaaagcca atgactcaac
    21181 aattactggc gaagcgaaga aaccgcaatt taaagaagtt aaaacagtaa aatacactgc
    21241 ttacagcaat gttttagata aagaagagca tttcattgat catatagttg taatgggtga
    21301 tgaacgctca gatattcaag gattatatat aaaagaatca atgcatatgc gttctgtaga
    21361 cgaactgtat acgcaaagaa ataagtttat aagcgattat gaaataccgc atttatatgt
    21421 cgatagagag gctacatggc ttgctagacc aaccaatttt gatgacccgc gtcaccctaa
    21481 ttggctagtt attgaagtat gtggtggtca aacagatagc aagcgtcaat tcttaatgaa
    21541 ccaaatacaa gctttaatac ggggtgtatg gttgttgtca ggaacagata aagaattatc
    21601 tgaaacgacg ttaaaggtag accctaatat ttggcgtagt atgaaagatt taattaatta
    21661 cgacttgatt aagcaaggta taccggatga cgcaaagtat gagcaagtca aaaagaaaat
    21721 gcttgagacg tacatcaaac gagatatatt gaaacgagaa aatattaaag aagtaactac
    21781 acaaacaaca ataagaatta gtgataaaac atcggttgac agtgcgtcaa caagaggacc
    21841 cactgcatca gacgaaaaac caagcatcgt tactgaaaaa agtccgttca cgttccagca
    21901 agcactggat agacaaatgt ctaggggtaa cccgaaaaaa tctcatacat ggggctgggc
    21961 taatgcaaca cgagcacaaa cgagctcagc aatgaatgtt aagcgaatat gggaaagtaa
    22021 cacacaatgc tatcaaatgc ttaatttagg caagtatcaa ggcgtttcag ttagtgcgct
    22081 taataagata ctcaaaggga aaggaacgct agacggacaa ggcaaagcat ttgcagaagc
    22141 ctgtaagaaa aacaacatta acgaaatcta tttgatcgcg cacgctttct tagaaagtgg
    22201 atacggaaca agtaacttcg ctagtggtag atacggtgca tataattact tcggtattgg
    22261 tgcattcgac aacgaccctg attatgcaat gaaatttgct aagaataaag gttggacaac
    22321 tccagcaaaa gcaatcatgg gcggtgctag cttcgtaaga aaggattaca tcaacaaagg
    22381 gcagaataca ctgtacagaa tcagatggaa tcctaagaat ccagctacgc accaatacgc
    22441 tactgctata gagtggtgcc aacatcaagc tagtacaatc gctaagctat ataaacaaat
    22501 cggcttaaaa ggtatctact ttataagaga taaatataaa taaagaggtg tataaatgta
    22561 caaaataaaa gatgttgaaa cgagaataaa aaatgatggt gttgacttag gtgacattgg
    22621 ctgtcgattt tacactgaag atgaaaatac agcatctata agaataggta tcaatgacaa
    22681 acaaggtcgt atcgatctaa aagcacatgg cttaacacct agattgcatt tgtttatgga
    22741 agatggctct atattcaaaa atgagcccct tattatcgac gatgttgtaa aagggttcct
    22801 tacctacaag atatctaaaa aggttatcaa acacgctggt tatgtacgtt gtaagctgtt
    22861 tttagagaaa gaagaaggaa aaatacatgt tgcaaacttt tctttcaata tcgttgatag
    22921 tggtattgaa tctgctgtag caaaagaaat cgatgttaaa ttggtagatg atgctattac
    22981 gagaatttta aaagataacg cgacagattt attgagcaaa gactttaaag agaaaataga
    23041 taaagatgtc atttcttaca tcgaaaagaa tgaaagtaga tttataggtg cgaaaggtga
    23101 taaaggcgaa ccgggacaac ctggtgcaaa aggtgaagca ggtaaaaaag gagaacaagg
    23161 cgtacccggt aaaaacggta ctgtagtatc aatcaatcct gacactaaaa tgtggcaaat
    23221 tgatggtaaa gatacagata tcaaagcaga acctgagtta ttggacaaaa tcaatatcgc
    23281 aaatgttgaa gggttagaaa ataaattgca agaagttgaa aaaatcaaag atacaactct
    23341 caacgactct aaaacgtata cggattcaaa aattgctgaa ctagttgata gcgcgcctga
    23401 atctatgaat acattaagag aattagcaga agcaatacaa aacaactcta tttcagaaag
    23461 tgtattgcaa cagattggct caaaagttag tacagaagat tttgagagat tcaagcaatc
    23521 attaaacagt ttgtatgcag ataaaaatca tagtcataca atcaaacaga ttgaaggatt
    23581 agaaaatgct ttatcaaaaa aatcagacat aaatcacagt catgatgaac gttatctttt
    23641 atcatcaaat gcttttacaa aagaggaagc agataaactt tatcaaccta tcggttcttc
    23701 gcagccgtca ctgaatattt ggacaggcag tgaaacagaa tataattatt tgtatcaaaa
    23761 agaccctaat acactttact taattaaggg gtgattttat ggaaggtaat tttaaaaatg
    23821 taaagaagct tatttacgaa ggcgaagaat atacaaaagt atatgctgga aatatccaag
    23881 tatggaaaaa gccttcatct tttgtaataa aacccttacc taaaaataaa tatccggata
    23941 gcatagaaga atcaacagca aaatggacaa taaatggagt tgaacctaat aaaagttatc
    24001 aggtgacaat agaaaatgta cgtagcggta taatgaggat ttcgcaaact aatttagggt
    24061 caagtgaatt aggaatatca ggagtcaata gcggagttgc aagtaaaaat atcaacttta
    24121 gtaatccttc agggacgttg tatgtcacta taagtgatgt ttattcagga tctccgacat
    24181 tgaccattga ataattttaa acgactaatt ttttagtcgt tttttatttt ggataaaagg
    24241 agcaaacaaa tggatatcgg tacaatcgta agaacaattt tattaatagt cgcatggatc
    24301 aatcagtttt tagcaatcaa acatatttct ccaatcccag ttgacgaagt gtttataagc
    24361 acagtcgtta ctgggattgt ttcaatttgg acgtggtgga agaataacaa ctttactcac
    24421 gcatctaaga aagggcaaca aaaaatttat gaagtaaaag ctggcattca gtcaactggt
    24481 ggcgcaccta aagtgaacgg agatgataac aatgccgtcg gttagaacat acagtcaagc
    24541 tattagctac cttaaaagcc tagagggtaa ggcgtggaat ccagacaatg catttggatg
    24601 tcaatgcttc gatactgcca accaatattg gctttactta tttaatcata ggttgaaagg
    24661 tgtgggcgct gcggacattc ctacatggaa tgatttcact aacgaagcaa ccgtttacga
    24721 aaatactgtg tcgtttcaag cattgccggg cgacgtcgtt atttttaacc gtaattatgg
    24781 cggtggttat ggtcatgtag gtattgtaat aagcgctacg ttagattcta taactatttt
    24841 agagcagaac tggctaggcg gtgcttactg gagcccacca gaagttacta caagacgcac
    24901 acacggctat gattttccta tgtggttcat tcgtccattc tatgcaaaag aaacgactgc
    24961 taataagcta agaagcgcag taacaccagt taaacaagat aagttatcaa aaggcaaaaa
    25021 aatcatgctt gtagctggtc atggtattgg cgcatactct aacgacccag gtgccgttgc
    25081 gaatggagaa aacgaaagag attttaaccg taaaaatatt atccctagag tgaaaaagta
    25141 tcttgagtca gtaggtaaca cagtattgtt atacggtggc aactcgatga atcaagattt
    25201 atatcaagat acattgtacg gtcaacgtgt tggaaactat aaagattatg gcatgtactg
    25261 gattaaaagc gaagtcaaac cggatgcaat catagagttt catttagatt ctgctagccc
    25321 acaagcaagt ggcgggcatg taatcattag cgatcgtttc ccagctgatg acattgacaa
    25381 ggcattaagt agtgcattag ataaaacagt gggtaaaata agaggtgtga cacctagagg
    25441 ggatttattg aacgctaacg tgtctgctga tcttaatctt aattatcgtt taatcgaatt
    25501 aggttttatc acatctacga aagatttaaa ctacattaaa aacaatttag acagcttcac
    25561 gaagcggatt gctgaagcca ttaacggcag acaaattgat gcgccaagta gtaagccaag
    25621 cgctgacaaa ataacatgga attggaaagg cgtattttat cctaatccag aaaaagctat
    25681 aagagtcaga aaaacagctg gattaaccgg cacagtcgtt gaagaagatt catggctata
    25741 cacaaaagat gattgggtaa aattcgacca agtcattaaa aaagatggct actggtggat
    25801 tagattcaaa tatcaacgtg agggctctag tactaacaat ttctattgtg cagtgtgtag
    25861 aattactgat aaggaacaaa agattaaaaa tgaaaaatat tggggcacga ttgagtgggc
    25921 ttaataggtt gtacctataa aaagaaaaga ggtaggttat tttcttccta cctctaaaaa
    25981 tgattatctt tctattgtta tatgagttat atctttagga ctaatcagtc tattttttac
    26041 attagaatct tgatctcctg ccttgccata tactttttca tcagaaggat ctttactatg
    26101 gatagttact ttatcaccga ctttaacaat atgcttttct tttaatttat ctactaattt
    26161 tttccatgca tcatttgcct ctattgtgtt tccgtttgga ttaactcttg taatatcgac
    26221 acggttaacg ctatcaggac taacggtgct gttattagta ttactaagat tatctaagtt
    26281 cgcagtccca gaaatttcgc tctctccacc gttttttaat ttatatttta ctttaatcgt
    26341 ttcgtggttt gttttatcaa tgatatttgc gtcttttaaa gcgtctctta catttttcca
    26401 caattcgcta tctgttattt cagaagcttt tgcaacgtta ttaataccat tataatttga
    26461 agaagaatga aaacctgaac ctactgttgt taaaactaaa gcacttgcta tcaatgtttt
    26521 tgttaatagt tttttattca ttttattttc tcctataact tatttgcaat cgat
//
LOCUS       DAMZKC010000021        26088 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.021, whole genome shotgun sequence.
ACCESSION   DAMZKC010000021 DAMZKC010000000
VERSION     DAMZKC010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 26088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26088
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.021"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            70..882
                     /gene="tagG"
                     /locus_tag="POB55_000624"
     CDS             70..882
                     /gene="tagG"
                     /locus_tag="POB55_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acids export ABC transporter permease
                     subunit TagG"
                     /protein_id="HDI6499610.1"
                     /translation="MSAIGTVFKEHVKNFYLIQRLAQFQVKIINHSNYLGVAWELINP
                     VMQIMVYWMVFGLGIRSNAPIHGVPFVYWLLVGISMWFFINQGILEGTKAITQKFNQV
                     SKMNFPLSIIPTYIVTSRFYGHLGLLLLVIIACMFTGIYPSIHIIQLLIYVPFCFFLT
                     ASVTLLTSTLGVLVRDTQMLMQAILRILFYFSPILWLPKNHGISGLIHEMMKYNPVYF
                     IAESYRAAILYHEWYFMDHWKLMLYNFGIVAIFFAIGAYLHMKYRDQFADFL"
     gene            981..2084
                     /locus_tag="POB55_000625"
     CDS             981..2084
                     /locus_tag="POB55_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HDI6499611.1"
                     /translation="MNVLIKKFYHLVVRILSKMITPQVIDKPHIVFMMTFPEDIKPII
                     KALNNSSYQKTVLTTPKQAPYLSELSDDVDVIEMTNRTLVKQIKALKSAQMIIIDNYY
                     LLLGGYNKTSNQHIVQTWHASGALKNFGLTDHQVDVSDKTMVQQYRKVYQATDFYLVG
                     CEQMSQCFKQSLGATEEQMLYFGLPRINKYYTADRATVKAELKDKYGITNKLALYVPT
                     YREDKADNRAIDKAYFEKCLPGYTLINKLHPSIEHSDIDDVSSIDTSTLMLMSDIIIS
                     DYSSLPIEASLLDIPTIFYVYDEGTYDKVRGLNQFYKAIPDSYKVYTEEDLIMTIQEK
                     EHLLNPLFKDWHKYNTDKSLHQLTEYIDKMVTK"
     gene            2081..3142
                     /locus_tag="POB55_000626"
     CDS             2081..3142
                     /locus_tag="POB55_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDI6499612.1"
                     /translation="MRLTIIIPTCNNEATIRQLLISIESKEHYRILCIDGGSTDQTIP
                     MIERLPRELKHISLIQLQNASIATCINKGLMDIKMTDPHDSDAFMVINPTSIVLPGKL
                     DRLTAAFKNNDNIDMVIGQRAYNYHGEWKLKSADEFIKDNRIVTLTEQPDLLSMMSFD
                     GKLFSAKFAELQCDETLANTYNHTILVKAMQKATDIHLVSQMIVGDNDIDTHATSNDE
                     DFNRYITEIMKIRQRVMEMLLLPEQRLLYSDMVDRILFNNSLKYYMNEHPAVTHTTIQ
                     LVKDYIMSMQHSDYVSQNMFDIINTVEFIGENWDREIYELWRQTLIQVGINRPTYKKF
                     LIQLKGRKFAHRTKSMLKR"
     gene            3205..3603
                     /gene="tagD"
                     /locus_tag="POB55_000627"
     CDS             3205..3603
                     /gene="tagD"
                     /locus_tag="POB55_000627"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HDI6499613.1"
                     /translation="MKRVITYGTYDLLHYGHIELLRRAREMGDYLIVALSTDEFNQIK
                     HKKSYYDYEQRKMMLESIRYVDLVIPEKGWGQKEDDVEKFDVDVFVMGHDWEGEFDFL
                     KDKCEVIYLKRTEGISTTKIKQELYGKDAK"
     gene            complement(3720..5015)
                     /gene="pbp4"
                     /locus_tag="POB55_000628"
     CDS             complement(3720..5015)
                     /gene="pbp4"
                     /locus_tag="POB55_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein PBP4"
                     /protein_id="HDI6499614.1"
                     /translation="MKNLISIIIILCLTLSIMTPYAQAANSDVTPVQAANQYGYAGLS
                     AAYEPTSAVNVSQTGQLLYQYNIDTKWNPASMTKLMTMYLTLEAVNKGQLSLDDTVTM
                     TNKEYIMSTLPELSNTKLYPGQVWTIADLLQITVSNSSNAAALILAKKVSKNTSDFVD
                     LMNNKAKAIGMKNTHFVNPTGAENSRLRSFAPTKYKDQERTVTTARDYAILDLHVIKE
                     TPKILDFTKQLAPTTHAVTYYTFNFSLEGAKMSLPGTDGLKTGSSDTANYNHTITTKR
                     GKFRINQVIMGAGDYKNLGGEKQRNMMGNALMERSFDQYKYVKILSKGEQRINGKKYY
                     VENDLYDVLPSDFSKKDYKLVVEDGKVHADYPREFINKDYGPPTVEVHQPIIQKANTV
                     AKSMWEEHPLFTIIGGACLVAGLALIVHMIINRLFRKRK"
     gene            5436..7163
                     /locus_tag="POB55_000629"
     CDS             5436..7163
                     /locus_tag="POB55_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6499615.1"
                     /translation="MKRENPLFFLFKKLSWPVGLIVAAITISSLGSLSGLLVPLFTGR
                     IVDKFSVSHINWNLIALFGGIFVINALLSGLGLYLLSKIGEKIIYAIRSVLWEHIIQL
                     KMPFFDKNESGQLMSRLTDDTKVINEFISQKLPNLLPSIVTLVGSLIMLFILDWKMTL
                     LTFITIPIFVLIMIPLGRIMQKISTSTQSEIANFSGLLGRVLTEMRLVKISNTERLEL
                     DNAHKNLNEIYKLGLKQAKIAAVVQPISGIVMLLTIAIILGFGALEIATGAITAGTLI
                     AMIFYVIQLSMPLINLSTLVTDYKKAVGASSRIYEIMQEPIEPTEALEDSENVLIDDG
                     VLSFEHVDFKYDVKKILDDVSFQIPQGQVSAFVGPSGSGKSTIFNLIERMYEIESGDI
                     KYGLESVYNIPLSKWRRKIGYVMQSNSMMSGTIRDNILYGINRHVSDEELINYAKLAN
                     CHDFIMQFDEGYDTLVGERGLKLSGGQRQRIDIARSFVKNPDILLLDEATANLDSESE
                     LKIQEALETLMEGRTTIVIAHRLSTIKKAGQIIFLDKGQVTGKGTHSELMASHAKYKN
                     FVVSQKLTD"
     gene            7520..8749
                     /locus_tag="POB55_000630"
     CDS             7520..8749
                     /locus_tag="POB55_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDI6499616.1"
                     /translation="MFLLINIIGLIVFLGIAVLFSRDRKNIQWQSIGILVVLNLFLAW
                     FFIYFDWGQKAVRGAANGIAWVVQSAHAGTGFAFASLTNVKMMDMAVAALFPILLIVP
                     LFDILMYFNILPKIIGGIGWLLAKVTRQPKFESFFGIEMMFLGNTEALAVSSEQLKRM
                     NEMRVLTIAMMSMSSVSGAIVGAYVQMVPGELVLTAIPLNIVNAIIVSCLLNPVSVEE
                     KEDIIYSLKNNEVERQPFFSFLGDSVLAAGKLVLIIIAFVISFVALADLFDRFINLIT
                     GMIAGWIGIKGSFGLNQILGVFMYPFALLLGLPYDEAWLVAQQMAKKIVTNEFVVMGE
                     ISKDIASYTPHHRAVITTFLISFANFSTIGMIIGTLKGIVDKKTSDFVSKYVPMMLLS
                     GILVSLLTAAFVGLFAW"
     gene            9004..9096
                     /locus_tag="POB55_000631"
     CDS             9004..9096
                     /locus_tag="POB55_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499617.1"
                     /translation="MFCYYKQHKGDNFSIEEVKNIIADNEMKVN"
     gene            9274..10107
                     /locus_tag="POB55_000632"
     CDS             9274..10107
                     /locus_tag="POB55_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDI6499618.1"
                     /translation="MNKTVKDLILVVLGSFIFAAGVNAFIISGNLGEGGVTGLAIILY
                     YAFHISPAITNFLVNAVLIAIGYKFLSKRSMYLTILVTILISIFLSLTESWQVETGNS
                     IVNAIFGGVSVGLGIGVIILAGGTTAGTTILARIATKYLDVSTPYALLFFDMIVVAIS
                     LTVIPLDKVLVTVISLYIGTKVMEYVIEGLNTKKAMTIISTNPDKLAKAIDEQIGRGL
                     TILNGHGYYTREEKDVLYVVISKTQVSKAKRLIKQIDKDAFLVIHDVRDVYGNGFLAD
                     E"
     gene            10389..11186
                     /locus_tag="POB55_000633"
     CDS             10389..11186
                     /locus_tag="POB55_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6499619.1"
                     /translation="MNRLHGQQVKIGYGDNTIINKLDVEIPDGKVTSIIGPNGCGKST
                     LLKALSRLLAVKEGEVFLDGENIHTQSTKEIAKKIAILPQSPEVADGLTVGELVSYGR
                     FPHQKGFGRLTAEDKKEIDWAMEVTGTDTFRHRSINDLSGGQRQRVWIAMALAQRTDI
                     IFLDEPTTYLDICHQLEILELVQKLNQEQGCTIVMVLHDINQAIRFSDHLIAMKEGDI
                     IATGSTEDVLTQEILEKVFNIDVVLSKDPKTGKPLLVTYDLCRRAYS"
     gene            11222..12226
                     /locus_tag="POB55_000634"
     CDS             11222..12226
                     /locus_tag="POB55_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDI6499620.1"
                     /translation="MTNRENSTPLKFLAYIIGLSMILLITLFISTLIGDAKIQASTII
                     EAIFNYNPSNQQQNIINEIRIPRNIAAVIVGMALAVSGAIIQGVTRNSLADPALIGLN
                     SGASFALALTYAVLPNTSFLILMFAGFLGAILGGAIVLMIGRSRRDGFNPMRIILAGA
                     AVSAMLTALSQGIALAFRLNQTVTFWTAGGVSGTTWSHLKWAIPLIGIALFIILTISK
                     QLTILNLGESLAKGLGQNVTMIRGICLIIAMILAGIAVAIAGQVAFVGLMVPHIARFL
                     IGTDYAKILPLTALLGGILVLVADVIARYLGEAPVGAIISFIGVPYFLYLVKKGGRSI
                     "
     gene            12223..13239
                     /locus_tag="POB55_000635"
     CDS             12223..13239
                     /locus_tag="POB55_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDI6499621.1"
                     /translation="MISSNNKRRQWIALAVFSILLFLGCTWSITSGEYNIPVERFFKT
                     LIGQGDAIDELILLDFRLPRMMITILAGAALSISGAIVQSVTKNPIAEPGILGINAGG
                     GFAIALFIAIGKINADNFVYVLPLISILGGIATALIIFIFSFNKNEGVTPASMVLIGV
                     GLQTALYGGSITIMSKFDDKQSDFIAAWFAGNIWGDEWPFVIAFLPWVLIIIPYLLFK
                     SNTLNIIHTGDNIARGLGVRLSRERLILFFIAVMLSSAAVAVAGSISFIGLMGPHIAK
                     RIVGPRHQLFLPIAILVGACLLVIADTIGKIVLQPGGVPAGIVVAIIGAPYFLYLMYK
                     TKNV"
     gene            13473..14441
                     /gene="dhaK"
                     /locus_tag="POB55_000636"
     CDS             13473..14441
                     /gene="dhaK"
                     /locus_tag="POB55_000636"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HDI6499622.1"
                     /translation="MMKKLINKKETFLTDMLEGLLIAHPELDLIANTVIVKKAKKEHG
                     VAIVSGGGSGHEPAHAGFVAEGMLDAAVCGEVFTSPTPDKILEAIKAVDTGDGVLLVV
                     KNYAGDVMNFEMAQELAEMEGINVQTVIVRDDIAVTNEVQRRGVAGTVFVHKLAGYLA
                     EKGYSLTEIKSRVEALLPEIKSIGMAIEPPLVPTTGKYGFDIEDDKMEIGIGIHGEKG
                     IHREEVKDIDHIVGTLLDELYKEVTANDVILMVNGMGGTPLSELNIVTKYIQQNLAAR
                     TVNVAKWFVGDYMTSLDMQGFSITIVPNKPEYLEAFLAPTTSQYFK"
     gene            14483..15067
                     /gene="dhaL"
                     /locus_tag="POB55_000637"
     CDS             14483..15067
                     /gene="dhaL"
                     /locus_tag="POB55_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HDI6499623.1"
                     /translation="MKVNDMKARLLNLEETFKKHESELTELDRAIGDGDHGVNMVRGF
                     SSLKDKLDDSSMQSLFKSTGMALMSNVGGASGPLYGFSFVKMSAVTKDDMDNQDFITL
                     IQAFAEAVESRGKVTLNEKTMYDVVARAAEKLENGETLTFNDLQQLADNTKDMVATKG
                     RAAYFGEESKGYIDPGAQSMVYILNALIGDEDNA"
     gene            15060..15422
                     /locus_tag="POB55_000638"
     CDS             15060..15422
                     /locus_tag="POB55_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HDI6499624.1"
                     /translation="MPKIILVSHSKEIASGTKSLLKQMAGDVDIIPIGGLPDGSIGTS
                     FDIIQEVLTKLEDDALCFYDIGSSEMNVDMAIEMYDGNHRVLKVDAPIVEGSFIAAVK
                     LSIGGSIDDALAEIKQSF"
     gene            15538..16035
                     /locus_tag="POB55_000639"
     CDS             15538..16035
                     /locus_tag="POB55_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499625.1"
                     /translation="MQHLIKKHVLNGEFDLVRQLMSETDFMEFEEAYISSAHEVESMM
                     FYTCILDMIKYEESSEMHDLAFLLLVYPLSEYEGALDSAYYHADASIKLTDGKEVKSL
                     LQMLLLHAIPTPVISDKKAFDIAKQILKLDPNNNVARNVLKDTAKRMDNVVVDINELH
                     QRNAR"
     gene            16492..17559
                     /locus_tag="POB55_000640"
     CDS             16492..17559
                     /locus_tag="POB55_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499626.1"
                     /translation="MMMNKEATKIGFAYVGIVVGAGFSTGQEVMQFFTKYGLWAYLGV
                     IISGFILAFIGRQVAKIGTAFEATNHESTLQYVFGEKFSKVFDYILIFFLFGIAVTMI
                     AGAGATFEESYNIPTWLGALIMTLAIYITLLLDFNKIVRALGIVTPFLIVLVVLIAGV
                     YLFKGHVSLAEVNQVVPEASIWKGIWFGTIYGGLAFSVGFSTIVAIGGDTEKRTVSGA
                     GAMYGGIIYTVLLALINFALQSEYPTIKNASIPTLTLANNIHPLIATVLSVIMLAVMY
                     NTILGLMYSFAARFTEPYSKNYHIFIIIMMVAGYLLSFVGFAELINKLYTIMGYVGLF
                     IVVAVIIKYFKRKNADKKHIA"
     gene            17710..18753
                     /locus_tag="POB55_000641"
     CDS             17710..18753
                     /locus_tag="POB55_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDI6499627.1"
                     /translation="MNKDNKWTMITALFITVISVLLAFHLKQHYDQITNENHANKDKI
                     NIKNKNVRIYQNLTYNRVFPNSKLDIITPVDMSSNAKLPVIFWMHGGGYIAGDKQYKN
                     PLLAKIAEQGYIVVNVNYALAPQYKYPTPLIQMNQATQFIKENKMNLPIDFNQVIIGG
                     DSAGAQLASQFTAIQTNDRLREAMKFDQSFKPSQIKGAILFGGFYNMQTVRETEFPRI
                     QLFMKSYTGEEDWEKSFKNISQMSTVKQSTKNYPPTFLSVGDSDPFESQNIEFSKKLQ
                     ELNVPVDTLFYDGTHHLHHQYQFHLNKPESIDNIKKVLLFLSRNTSSSGIQTEEKPQI
                     ENPSNELPLNPLN"
     gene            19082..19510
                     /locus_tag="POB55_000642"
     CDS             19082..19510
                     /locus_tag="POB55_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499628.1"
                     /translation="MTRSALKPFKNKRVMVTGRIQRVLFKNYLDRHSTFKPNVRILLK
                     DVFVSGVSIDHLWLYETNKYYALAMELIHQRVKFSANVVPYYKINRNNNLFVQDYGIK
                     RKGRLITEEAYNQNNQYQDKIYEKLPDIDFRLEDFYSKEN"
     gene            complement(19719..20225)
                     /locus_tag="POB55_000643"
     CDS             complement(19719..20225)
                     /locus_tag="POB55_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6499629.1"
                     /translation="MAHIIRRVSIKDVENFISMLAKIYDESPYMFYTPGEYDPSVTSA
                     SKQLEEYITSPHKVIFVAESDEQLVGFAFVNTTPFQRIKHVAKIDLGVKKLYQHRGIG
                     QALLDAIMAWCLNNQIHRIEANVPLNNQPALELFKSADFQIEGVLKDKLFIDGKYYDD
                     YMMAKILN"
     gene            20338..21261
                     /gene="graX"
                     /locus_tag="POB55_000644"
                     /gene_synonym="apsX"
     CDS             20338..21261
                     /gene="graX"
                     /locus_tag="POB55_000644"
                     /gene_synonym="apsX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="auxiliary protein GraX/ApsX"
                     /protein_id="HDI6499630.1"
                     /translation="MKPKVLLAGGTGYIGKYLSEVIENDAELFTISKYPDNKKTDDVE
                     MTWIQCDIFHYEQVVAAMNQIDIAVFFIDPTKNSAKITQSSARDLTLIAADNFGRAAA
                     INQVKKVIYIPGSRYDNETIERLGAYGTPVETTNLVFKRSLVNVELQVSKYDDVRSTM
                     KVVLPKGWTLKNVVNHFIAWMGYTKGTFVKTEKSHDQFKIYIKNKVRPLAVFKIEETA
                     DGIITLILLSGSLVKKYTVNQGKLEFRLIKESAVVYIHLYDYIPRLFWPIYYFIQAPM
                     QKMMIHGFEVDCRIKDFQSRLKSGENMKYTK"
     gene            21277..21951
                     /gene="graR"
                     /locus_tag="POB55_000645"
                     /gene_synonym="apsR"
     CDS             21277..21951
                     /gene="graR"
                     /locus_tag="POB55_000645"
                     /gene_synonym="apsR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor
                     GraR/ApsR"
                     /protein_id="HDI6499631.1"
                     /translation="MQILLVEDDNTLFQELKKELEQWDFNVAGIEDFGKVMDTFESFN
                     PEIVILDVQLPKYDGFYWCRKMREVSNVPILFLSSRDNPMDQVMSMELGADDYMQKPF
                     YTNVLIAKLQAIYRRVYEFTAEEKRTLTWQDAVVDLSKDSIQKGDDTIFLSKTEMIIL
                     EILITKKNQIVSRDTIITALWDDEAFVSDNTLTVNVNRLRKKLSEISMDSAIETKVGK
                     GYMAHE"
     gene            21944..22984
                     /gene="graS"
                     /locus_tag="POB55_000646"
                     /gene_synonym="apsS"
     CDS             21944..22984
                     /gene="graS"
                     /locus_tag="POB55_000646"
                     /gene_synonym="apsS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase GraS/ApsS"
                     /protein_id="HDI6499632.1"
                     /translation="MNNLKWVAYFLKSRMNWIFWILFLNLLMLGISLIDYDFPIDSLF
                     YIVSLNLSLTMIFLILTYFKEVKLYKHFDKDKEIEEIKHKDLAETPFQRHTVDYLYRQ
                     ISAHKEKVVEQQLQLNMHEQTITEFVHDIKTPVTAMKLLIDQEKNQERKQALLYEWSR
                     INSMLDTQLYITRLESQRKDMYFDYVSLKRMVIDEIQLTRHISQVKGIGFDVDFKVDD
                     YVYTDIKWCRMIIRQILSNALKYSENFNIEIGTELNDQHVSLYIKDYGRGISKKDMPR
                     IFERGFTSTANRNETTSSGMGLYLVNSVKDQLGIHLQVTSTVGKGTTVRLIFPLQNEI
                     VERMSEVTNLSF"
     gene            23128..23889
                     /gene="vraF"
                     /locus_tag="POB55_000647"
     CDS             23128..23889
                     /gene="vraF"
                     /locus_tag="POB55_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein VraF"
                     /protein_id="HDI6499633.1"
                     /translation="MAILEVKQLTKIYGTKKMAQEVLRDINMSIEEGEFIAIMGPSGS
                     GKTTLLNVLSSIDYISQGSITLKGKKLEKLSNKELSDIRKHDIGFIFQEYNLLHTLTV
                     KENIMLPLTVQKLDKEHMLNRYEKVAEALNILDISDKYPSELSGGQRQRTSAARAFIT
                     LPSIIFADEPTGALDSKSTQDLLKRLTRMNEAFKSTIIMVTHDPVAASYANRVVMLKD
                     GQIFTELYQGDDDKHTFFKEIIRVQSVLGGVNYDL"
     gene            23879..25768
                     /gene="vraG"
                     /locus_tag="POB55_000648"
     CDS             23879..25768
                     /gene="vraG"
                     /locus_tag="POB55_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease VraG"
                     /protein_id="HDI6499634.1"
                     /translation="MTFNEIIFKNFRQNLSHYAIYLFSLITSVVLYFSFVALKYAHKL
                     NMTESYPIIKEGSQVGSYFLFFIIIAFLLYANVLFIKRRSYELALYQTLGLSKFNIIY
                     ILMLEQLLIFIITAILGIIIGIFGSKLLLMIVFTLLGIKEKVPIIFSLRAVFETLMLI
                     GVAYFLTSAQNFILVFKQSISQMSKNNQVKETNHNKITFEEVVLGILGIVLITTGYYL
                     SLNIVQYYDSIGILMFILLSTVIGAYLFFKSSVSLVFKMVKKFRKGVISVNDVMFSSS
                     IMYRIKKNAFSLTVMAIISAITVSVLCFAAISRASLSSEIKYTAPHDVTIKDQQKANQ
                     LASELNNQKIPHFYNYKEVIHTKLYKDNLFDVKAKEPYNVTITSDKYIPNTDLKRGQA
                     DLFVAEGSIKDLVKHKKHGKAVIGTKKHHVNIKLRKDINKIYFMTDVDLGGPTFVLND
                     KDYQEIRKYTKAKHIVSQFGFDLKHKKDALALEKVKNKVDKSIKTRSEAISSISSLTG
                     ILLFVTSFLGITFLIAVCCIIYIKQIDETEDELENYSILRKLGFTQKDMARGLKFKIM
                     FNFGLPLVIALSHAYFTSLAYMKLMGTTNQIPVFIVMGLYICMYAVFAVTAYNHSKRT
                     IRHSI"
BASE COUNT         9105 a         3542 c         4754 g         8687 t
ORIGIN      
        1 aacaattatt ataatactat ttagttaagt agagaaatag ttaaacattt gaaagtgtgg
       61 tttaatggaa tgtcagcaat aggaacagtt tttaaagaac atgtaaagaa cttttattta
      121 attcaaagac tggctcagtt tcaagttaaa attatcaatc atagtaacta tttaggtgtg
      181 gcttgggaat taattaaccc tgttatgcaa ataatggttt actggatggt ttttggatta
      241 ggaataagaa gtaatgcacc aattcatggt gtaccttttg tttattggtt attggttggt
      301 atcagtatgt ggttcttcat caaccaaggt attttagaag gtactaaagc aattacacaa
      361 aagtttaatc aagtatcgaa aatgaacttc ccgttatcga taataccgac atatattgtg
      421 acaagtagat tttatggaca tttaggctta cttttacttg tgataattgc atgtatgttt
      481 actggtattt atccatcaat acatatcatt caattattga tatatgtacc gttttgtttt
      541 ttcttaactg cctcggtgac gttattaaca tcaacactcg gtgtgttagt tagagataca
      601 caaatgttaa tgcaagcaat attaagaata ttattttact tttcaccaat tttgtggcta
      661 ccaaagaacc atggtatcag tggtttaatt catgaaatga tgaaatataa tccagtttac
      721 tttattgctg aatcataccg tgcagcaatt ttatatcacg aatggtattt catggatcat
      781 tggaaattaa tgttatacaa tttcggtatt gttgccattt tctttgcaat tggtgcgtac
      841 ctacacatga aatatagaga tcaatttgca gacttcttgt aatatattta tatgacgaaa
      901 ccccgctaac cattaataaa tggaagtggg gttcattttt gtttataatt taagtaaata
      961 acatattaag ttggtgtatt atgaacgttt taataaagaa attttatcat ttggtagttc
     1021 gaatactttc taaaatgatt acgcctcaag tgattgataa accgcatatc gtatttatga
     1081 tgacttttcc agaagatatt aagcctatca tcaaagcatt aaataattcg tcgtatcaga
     1141 aaactgtttt aacaacacca aaacaagcgc cttatttatc tgaacttagc gacgatgttg
     1201 atgtgataga aatgactaat cgaacattgg taaaacaaat taaggctttg aaaagcgcgc
     1261 agatgattat tatcgataat tattacctat tactaggtgg atataataag acttctaatc
     1321 aacacattgt tcaaacgtgg catgcaagtg gtgcattaaa aaactttgga ttaacagatc
     1381 atcaagtcga tgtgtctgac aagacaatgg ttcagcagta ccgtaaagtt tatcaagcga
     1441 cggattttta cttagtgggt tgtgaacaaa tgtcacaatg ttttaaacag tctttaggtg
     1501 caacagaaga gcaaatgttg tattttgggc ttccgagaat taataaatat tacacagctg
     1561 atagagcaac ggttaaggca gagttaaagg ataaatatgg aattacaaat aagttggcat
     1621 tatatgtacc aacatataga gaagataaag cagataatag ggctattgat aaagcttatt
     1681 ttgaaaaatg tttaccagga tatacactga ttaataaatt acatccatca attgaacatt
     1741 cagacattga tgacgtatct tcaatcgaca cgtctacatt aatgctaatg tcagatataa
     1801 ttattagcga ctatagttcg ctgccaatag aagctagctt gttagatatt ccaactatat
     1861 tttatgtgta tgatgaagga acatatgata aagtgagagg cctgaatcaa ttttacaaag
     1921 caataccgga tagctacaaa gtgtatactg aagaagattt aataatgacg atacaagaaa
     1981 aagaacatct attaaatccg ttatttaaag attggcataa gtataatact gataaaagtt
     2041 tacatcagct cacagaatat atagataaga tggtgacaaa atgaggctta cgataatcat
     2101 acctacatgt aataatgagg caacaattcg acaattgtta atatctattg agagtaaaga
     2161 acactataga atcctttgta ttgatggtgg ttctactgat caaacaattc ctatgattga
     2221 acggttacca agagaactca agcatatttc attaatacaa ttacaaaatg cttcgatagc
     2281 tacgtgtatt aataaaggtt tgatggatat caaaatgaca gatccacatg atagtgacgc
     2341 atttatggtc ataaatccaa catcaatcgt attgccaggt aaattagata ggttaactgc
     2401 agctttcaaa aataatgata atattgatat ggtaataggg cagcgagctt acaattacca
     2461 tggtgaatgg aaattgaaaa gtgctgatga gtttattaaa gataatcgaa tcgttacatt
     2521 aacggaacaa ccagatttgt tatcaatgat gtcttttgac ggaaagttat tcagtgctaa
     2581 atttgctgaa ttacagtgtg acgaaacttt agctaacaca tacaatcaca caatacttgt
     2641 caaggcgatg caaaaagcta cggatataca tttagtttca cagatgattg tcggagataa
     2701 cgatatagat acacatgcta caagtaacga tgaagatttt aatagatata tcacagaaat
     2761 tatgaaaata agacaacgag tcatggaaat gttactatta cctgaacaaa ggctattata
     2821 tagtgatatg gttgatcgta ttttattcaa taattcatta aaatattata tgaacgaaca
     2881 cccagcagta acgcacacga caattcaact cgtaaaagac tatattatgt ctatgcagca
     2941 ttctgattat gtatcgcaaa acatgtttga cattataaat acagttgaat ttattggtga
     3001 gaattgggat agagaaatat acgaattgtg gcgacaaaca ttaattcaag tgggcattaa
     3061 taggccgact tataaaaaat tcttgataca acttaaaggg agaaagtttg cacatcgaac
     3121 aaaatcaatg ttaaaacgat aacgtgtaca ttgatgacca taaactgcaa tcctatgatg
     3181 tgacaatatg aggaggataa cttaatgaaa cgtgtaataa catatggcac atatgactta
     3241 cttcactatg gtcatatcga attgcttcgt cgtgcaagag agatgggcga ttatttaata
     3301 gtagcattat caacagatga atttaatcaa attaaacata aaaaatctta ttatgattat
     3361 gaacaacgaa aaatgatgct tgaatcaata cgctatgtcg atttagtcat tccagaaaag
     3421 ggctggggac aaaaagaaga cgatgtcgaa aaatttgatg tagatgtttt tgttatggga
     3481 catgactggg aaggtgaatt cgacttctta aaggataaat gtgaagtcat ttatttaaaa
     3541 cgtacagaag gcatttcgac gactaaaatc aaacaagaat tatatggtaa agatgctaaa
     3601 taaattatat agaactatcg atactaaacg ataaattaat gtaggttatt ataaaataaa
     3661 tataaaacgg acaagtttcg cagctttata atgtgcaact tgtccgtttt tagtatgttt
     3721 tattttcttt ttctaaataa acgattgatt atcatatgaa caattagtgc taatccagcg
     3781 acaaggcatg caccaccaat gatagtgaat aatggatgtt cttcccacat acttttagca
     3841 acagtatttg ccttttgaat aattggctga tgaacttcta cagttggagg tccataatct
     3901 ttattaataa attctcttgg atagtccgcg tgtactttac catcttcgac tacaagttta
     3961 taatcttttt tactaaaatc acttggtaaa acatcgtaaa gatcgttttc aacataatat
     4021 ttcttaccat ttatcctttg ctcaccttta gacaatattt ttacatattt atactgatca
     4081 aatgagcgtt ccattaatgc attccccatc atattacgtt gcttctcgcc accaaggttt
     4141 ttatagtctc ccgcacccat gataacttga ttaattctaa atttacctcg tttggtagta
     4201 atcgtatggt tgtaatttgc tgtatcactt gatccagttt ttaaaccatc tgtacccggc
     4261 aaactcattt ttgcaccttc caatgaaaag ttgaatgtgt aatacgtaac tgcatgcgtt
     4321 gttggtgcta actgctttgt aaagtctaat attttaggtg tctctttaat cacgtgtaaa
     4381 tctaaaatgg catagtctct agcagtcgtt acagtacgtt cttggtcttt atactttgtt
     4441 ggtgcaaatg aacgtaatct tgaattttca gcacccgttg gattgacgaa atgtgtattt
     4501 ttcattccga tagctttagc tttgttattc attaaatcaa cgaaatcgct ggtgtttttt
     4561 gataccttct tagctaaaat taatgccgcg gcattactag aattagatac tgtaatttgt
     4621 aataggtctg cgattgtcca tacttgtcca ggatatagtt tcgtattact caactcaggt
     4681 agtgtagaca taatatattc tttgttcgtc attgtgacag tgtcatcaag tgaaagctgc
     4741 cccttattta cagcttccaa tgttaagtac attgtcatta atttggtcat agacgctgga
     4801 ttccacttag tatcgatatt gtattgatac agtaattgtc cagtttgact tacattaaca
     4861 gcactcgtcg gttcgtatgc agctgacaaa cctgcataac catattgatt tgctgcttgt
     4921 acaggggtta cgtcactgtt agcagcttgt gcatatggtg tcataatact taatgttaaa
     4981 cataaaatga tgataataga tattaaattt ttcataaagc gttaatcttc ccttttccaa
     5041 ttcttaaata ttccctaaaa gcaatggtta ttcctactta cggaaatcat tgctaattca
     5101 cttcacctta attaaattgt tgaaaataaa gttttctgca gttaatttga aaaataatgc
     5161 aaatatatta cgtgtgtagc taaaggtgtt ataatgtttg tacgaagagc aaacttactc
     5221 aaaagcgatt aattttcatg ttttaatata aagactttga gaagttatta caaaaaatgc
     5281 aatagaaata ttctatcata taaatgttat gagcggtatt ttggggcaac actttatttg
     5341 atttttaaag ttttgttggg agaaagtata tgatagaaat gcatgtatct atctaaatga
     5401 attaactata aatttcaaac agaagaggta aaactatgaa acgagaaaat ccattgtttt
     5461 tcttatttaa aaaactatca tggccagtgg gtcttatcgt tgcagctatc actatttcat
     5521 cactagggag cttaagtgga ctattagtgc cactgtttac tggacgaatt gtagataaat
     5581 tttccgtgag ccatatcaat tggaatctaa tcgcattatt tggtggtatc tttgttatca
     5641 atgctttatt aagcggatta ggtttatatt tattaagtaa aattggtgaa aagattattt
     5701 atgcgatacg ctcagtttta tgggagcata tcatacaatt aaaaatgcca ttctttgaca
     5761 aaaatgaaag tggtcaatta atgagtcgat taactgacga tacgaaagtg ataaatgaat
     5821 ttatttcaca aaagctacct aacttattac catcaatcgt tacattagtt gggtcactaa
     5881 tcatgttatt tattttagat tggaaaatga cattattaac atttataacg ataccgatat
     5941 tcgttttaat tatgattcct ctaggtcgta ttatgcaaaa gatatcgaca agtacacaat
     6001 ctgaaattgc aaacttcagt ggtttgttag ggcgtgtcct aactgaaatg cgtcttgtta
     6061 aaatatcaaa tacagagcgt cttgaattag ataatgcaca taaaaatttg aatgaaatat
     6121 ataaattagg tttaaaacag gctaaaattg cggcagttgt acaaccaatt tcaggtatag
     6181 ttatgttgct aacaattgca attattttag gttttggtgc attagaaatt gcgactggtg
     6241 caatcactgc aggtacatta attgcaatga tattttatgt tattcagtta tctatgcctt
     6301 taatcaatct ttcaacgtta gttacagatt ataaaaaggc agtcggtgca agtagtagaa
     6361 tatacgaaat catgcaagaa cctattgaac cgacagaagc tcttgaagat tctgaaaatg
     6421 tattaattga tgacggtgta ttgtcatttg aacatgtaga ctttaaatat gatgtgaaga
     6481 aaatattaga tgatgtgtcg ttccaaatcc cacaaggtca agtgagtgct tttgtaggtc
     6541 cttctgggtc tggtaaaagt acgatattta atctgataga acgtatgtat gaaattgagt
     6601 caggtgatat taaatatggc cttgaaagtg tctataatat cccgttatct aagtggcgac
     6661 gcaaaattgg atatgttatg caatcaaatt cgatgatgag tggtacaatt agagacaata
     6721 ttttatacgg aattaatcgt catgtttcag atgaagaact tattaattat gctaaattag
     6781 cgaactgtca tgattttatc atgcaatttg atgaaggata tgacacgctt gtaggtgaac
     6841 gaggattgaa actgtctggc ggacaacgtc aacgtattga tattgctaga agttttgtta
     6901 aaaatcctga tattttgtta cttgatgaag caacagctaa tctcgatagt gaaagtgaat
     6961 tgaaaattca agaagcttta gaaacattga tggaaggtag aacaacgatt gtcattgcgc
     7021 atcgtttgtc tacaattaaa aaagccggtc aaattatatt cttagacaaa ggacaggtaa
     7081 caggtaaagg tacgcattca gaactgatgg catcacatgc gaagtataaa aactttgtag
     7141 tgtctcaaaa attaacagat taattttata tataagtaag cttggagcaa atacacatat
     7201 accatcgagg aaattaaagt gtggcacatt gatggatata gatgttaata aattgcttca
     7261 agcttttgtc tattttaaat catttgagaa gttacgacat aataattctt aaattaatga
     7321 aatcgatatt ttaagaaaaa aatgctcatg gtataataca agttataagc aaacatacat
     7381 atattaaata ctgtagccac gagtcataat tcttcatatt ttacatagca atttaactga
     7441 ttttagagtc catggtacag aagtttgata tttcaatgtt tctaaatttt taaaaaatta
     7501 aatcataggt gggtgccaaa tgtttttatt aatcaacatt attggtctaa ttgtatttct
     7561 tggtattgcg gtattatttt caagagatcg caaaaatatc caatggcaat caattgggat
     7621 cttagttgtt ttaaacctgt ttttagcatg gttctttatt tattttgatt ggggtcaaaa
     7681 agcagtaaga ggagcagcca atggtatcgc ttgggtagtt cagtcagcgc atgctggtac
     7741 aggttttgca tttgcaagtt tgacaaatgt taaaatgatg gatatggctg ttgcagcctt
     7801 attcccaata ttattaatag tgccattatt tgatatctta atgtacttta atattttacc
     7861 gaaaattatt ggaggtattg gttggttact agctaaagta acaagacaac ctaaattcga
     7921 gtcattcttt gggatagaaa tgatgttctt aggaaatact gaagcattag ccgtatcaag
     7981 tgagcaacta aaacgtatga atgaaatgcg tgtattaaca atcgcaatga tgtcaatgag
     8041 ctctgtatcg ggagctattg taggtgcgta tgtacaaatg gtaccaggag aactggtact
     8101 aacggcaatt ccactaaata tcgttaacgc gattattgtg tcatgcttgt tgaatccagt
     8161 aagtgttgaa gagaaagaag atattattta cagtcttaaa aacaatgaag ttgaacgtca
     8221 accattcttc tcattccttg gagattctgt attagcagca ggtaaattag tattaatcat
     8281 catcgcattt gttattagtt ttgtagcgtt agctgatcta tttgatcgtt ttatcaattt
     8341 gattacagga atgatagcag gatggatagg cataaaaggt agtttcggtt taaaccaaat
     8401 tttaggtgtg tttatgtatc catttgcgct attactcggt ttaccttatg atgaagcgtg
     8461 gttggtagca caacaaatgg ctaagaaaat tgttacaaat gaatttgttg ttatgggtga
     8521 aatttctaaa gatattgcat cttatacacc acaccatcgt gcggttatta caacattctt
     8581 aatttcattt gcaaacttct caacgattgg tatgattatc ggtacattga aaggcattgt
     8641 tgataaaaag acatcagact ttgtatctaa atatgtgcct atgatgctat tatcaggtat
     8701 cctagtttca ttattaacag cagctttcgt tggtttattt gcatggtaat atgttgaaga
     8761 gtgactatga taatacattt taactaataa atatgtccag gcatgtcgtc tattaatata
     8821 ggtgagatgc ttggactttt ttattattgg tataaagata tttaaatatt tttaaagtta
     8881 ccgaaattga agcattataa aaaccaagtg cacatggtaa tacacttggc ttttatggga
     8941 aatgaatatt attgtacata tgacagtaag gactaggtac agtcatagta cttcgagcaa
     9001 aatttgtttt gttattataa acaacacaaa ggagataact tctctattga agaagttaaa
     9061 aacattatag cagacaatga aatgaaagta aattaaaaat tcagaatatt tttaattatt
     9121 atattgtgag tgatatttat tagggaaagc tattcttcat ataaattagt taaatagtaa
     9181 atctttgtta gaactctttc catagagttg tgacagcatt tttgttgtgc tactattttt
     9241 atagtctaat aaatatataa aggggatggt ttcgtgaata aaacggttaa agatttaata
     9301 ctagttgtct taggttcatt tatctttgct gcaggtgtaa atgcatttat tatttctggt
     9361 aacttaggtg aaggcggggt tacaggttta gcaattattt tatattatgc gtttcatatt
     9421 tcaccagcca tcactaactt cttggtcaac gcagtattga ttgccatagg ttataaattt
     9481 ttgagtaaga gaagtatgta cttaactatt cttgtaacaa ttcttatttc aatatttttg
     9541 agtttaacag aatcatggca agtagaaact ggaaacagca ttgtgaatgc catttttggt
     9601 ggtgtaagcg ttggactagg aatcggagta attatccttg caggcggtac aacagcaggt
     9661 acaacaattt tggcgagaat tgcaacgaaa tacctcgatg taagcacgcc atatgctttg
     9721 cttttcttcg atatgatcgt tgttgcaatt tcacttacag ttattccact tgataaagta
     9781 ttagtaacag taatatcact ttatatagga acaaaagtga tggaatatgt catagaaggt
     9841 ttaaacacta aaaaagctat gacgattatt tcaactaatc ccgacaaact tgccaaagca
     9901 atagacgagc aaattggaag aggtttaacc attttaaacg gacatggcta ttatacgcgt
     9961 gaagaaaaag atgtcttata cgttgttatt tctaaaacac aagtttcaaa agcaaagcga
    10021 ttaattaaac aaatcgataa agatgcattc ctcgtaattc atgatgtaag agatgtctat
    10081 ggtaatggct ttcttgcaga tgaataaata aatggtatga gcacacatac ttaaatagaa
    10141 gtccacggac aagtttttga actatgaaga cttatctgtg ggcgtttttt attttataaa
    10201 agtaatatat aagacatgac aaatcgagct atccaattta aaaagtaatg ttagtcaata
    10261 agattgaaaa atgttataat gatgttcatg ataatcatta tcaattggga tgcctttgaa
    10321 aattgataat ttaaaaatag aaattatttt ttataaacag aaagaatttt attgaaagta
    10381 gggaaattat gaatcgtttg catggacaac aagttaaaat tggttacggg gataacacga
    10441 ttataaataa attagatgtt gaaataccag atggcaaagt gacgtcaatc attggtccta
    10501 acggctgcgg gaaatctact ttgctaaagg cattgtcacg tttattggca gttaaagaag
    10561 gcgaagtatt tttagatggt gaaaatattc atacacaatc tacgaaagag attgcaaaaa
    10621 aaatagccat tttacctcaa tcacctgaag tagcagatgg cttaactgtt ggggaattag
    10681 tttcatatgg tcgttttcca catcaaaaag gatttggtag attaactgct gaggataaga
    10741 aagaaattga ttgggcaatg gaagttacag gaactgatac attccgacac cgttcaatca
    10801 atgatttaag tggtggtcaa agacaacgtg tttggattgc aatggcatta gcacaaagaa
    10861 ctgatattat ctttttagac gaaccaacaa catatttaga tatctgtcat caattagaaa
    10921 tactagaatt agttcagaag ctaaatcagg aacaaggttg tacaattgtc atggttcttc
    10981 atgatatcaa ccaagcgatt cgtttctcag atcatcttat tgcgatgaaa gaaggggata
    11041 tcatcgctac aggttcaaca gaagacgtat taacacagga aattttagaa aaagttttca
    11101 atattgatgt tgttttaagt aaagatccta aaactggaaa acctttattg gtaacttatg
    11161 acttatgtcg cagagcttat tcttaattaa gtaagttaat atgataaaaa ggacaattaa
    11221 catgacaaat agagagaatt caacgccatt gaagttttta gcctatatta taggtttaag
    11281 tatgatacta ctaatcacac tatttatttc tacattaata ggtgacgcca aaattcaagc
    11341 ctctacaatt atagaggcta tttttaatta taatcctagc aatcaacagc aaaacatcat
    11401 caatgagatt aggattccca gaaatatagc agcagtaatt gtgggtatgg cgcttgcagt
    11461 ttctggtgcg attatacaag gtgttacccg taatagtctt gctgatccgg cgctcatagg
    11521 tttaaattca ggtgcttcat ttgctttagc attaacatat gcagttttac caaacacttc
    11581 atttttaata ttgatgtttg ctggattttt aggtgctatt ctaggaggtg ctattgtatt
    11641 aatgataggc cgatctagac gtgatggatt taatccgatg cgtattattt tagcgggtgc
    11701 agcagtaagt gctatgttaa cagcgctaag tcaaggtatt gcattagctt ttagactaaa
    11761 tcaaacagta acattttgga ctgctggagg cgtttcaggc acaacatggt cacaccttaa
    11821 gtgggcaatt ccattaattg gtattgcgtt attcattata ttaacaatta gtaaacaact
    11881 taccatttta aatcttggtg aatcattagc taaaggttta ggtcaaaatg taacaatgat
    11941 cagaggcata tgtttaatta ttgctatgat tctagcaggt attgcagttg ctatcgctgg
    12001 acaagttgca tttgtaggtt tgatggtacc tcatatagca agatttttaa ttggaactga
    12061 ttatgctaaa attctaccat taacagcctt gttaggtggg atactcgtgc ttgttgccga
    12121 tgtgatagca cgatatttag gagaagcgcc tgttggtgca atcatttcat ttatcggtgt
    12181 tccttacttt ttatatttag ttaaaaaagg aggacgctca atatgattag ttcaaataat
    12241 aaacgcagac aatggattgc actggctgtt tttagcattc tactatttct aggttgtact
    12301 tggagtatta cctcaggtga atacaacata cctgttgaaa gatttttcaa aactttaatt
    12361 ggacaaggtg atgccattga tgagttaatc ttattagatt tcagattacc tcggatgatg
    12421 attactattt tggctggcgc agcgcttagt attagtggtg caatagtgca aagtgtcacg
    12481 aaaaatccaa tagctgaacc cggtatatta ggtattaacg caggtggcgg atttgcaatc
    12541 gcattattta ttgcaattgg taaaattaat gctgacaact ttgtttatgt actgccgtta
    12601 ataagtatac taggtggtat cgccactgca ttgattattt ttattttcag ttttaataaa
    12661 aatgaaggtg ttacacctgc gagtatggta ttaataggtg taggtttaca aacagcatta
    12721 tatggtggct caattacaat tatgtcaaaa tttgatgata agcaatctga tttcatcgct
    12781 gcttggtttg caggtaatat ttggggtgac gaatggccat ttgtcattgc atttttaccg
    12841 tgggtgttga ttattattcc ttacttacta tttaaatcga atacactaaa tattattcat
    12901 acgggtgata atattgcacg aggtctaggt gtaaggttaa gcagagaacg tttaatatta
    12961 ttctttatcg cagtgatgtt atcatctgct gctgtagcag tagcaggttc gatttcgttt
    13021 atcggattaa tgggtccgca tattgccaaa cgtatcgttg gaccacgtca ccagttgttt
    13081 ttaccaattg ccattttagt aggggcatgt ttacttgtta tagctgatac aattggcaaa
    13141 attgtattac aaccaggtgg ggttccagca ggtattgtcg tagcaattat tggtgcaccg
    13201 tatttcttat atttaatgta caaaacgaaa aatgtatagt gtcaaaggac acaacttatt
    13261 gctatgaaag gcactttatt ataaggcttt tcatagcatt ttttatttaa tgagccactc
    13321 aagactattt attttttcaa taatgaacca ttaagttatc aagaggatct tatcaaaaat
    13381 atatttgata acggtatcag gttaattctt tatgatagcg cattcattta ttctgtttta
    13441 tactatgact gataatacca aggaggtaca acatgatgaa aaagttaatc aataaaaaag
    13501 aaacattttt aactgatatg cttgaaggat tgttaattgc gcacccagag ttagatctga
    13561 ttgctaatac agttattgta aaaaaagcta agaaagaaca tggtgtagca atagtctctg
    13621 gaggtggaag cggacatgaa cctgcgcatg ccggttttgt tgcagaaggt atgctagatg
    13681 cagcggtttg tggcgaagta tttacatcac ctacacctga taaaatatta gaagctatta
    13741 aagcagtaga tactggtgat ggtgtattac tagttgtaaa aaactatgca ggtgacgtga
    13801 tgaatttcga aatggcacaa gagcttgcag aaatggaagg tataaatgtt caaactgtta
    13861 ttgttcgtga cgacattgct gtgacaaacg aagtacaacg tcgtggtgtt gcaggaacag
    13921 tgtttgttca taagcttgcc ggttatcttg ctgaaaaagg ttattcatta acagagataa
    13981 aatcgcgtgt agaagcgttg ttacctgaaa ttaaaagtat tggtatggca attgagccac
    14041 cgcttgttcc aactactgga aaatatggct ttgatattga agacgacaaa atggaaatcg
    14101 gtattggtat acatggtgaa aaaggtattc atagggaaga agtaaaggat attgatcata
    14161 ttgttggaac attgttagac gaattgtata aagaagttac tgccaatgat gtcatattaa
    14221 tggtaaatgg tatgggtggt acgccgttat ctgaattaaa tatcgtaact aaatatattc
    14281 aacaaaattt agctgcaaga acggttaatg ttgctaaatg gtttgttggt gattatatga
    14341 catctttaga catgcaaggt ttttctataa ctatcgtgcc taataaacca gaatatttgg
    14401 aagcattttt agcaccaaca acaagtcaat actttaaata agaagtgaat atgaataaat
    14461 atacatttat gaggtggcac aaatgaaagt gaatgatatg aaagcacgtt tattaaattt
    14521 agaagaaacg tttaaaaaac atgaatctga attaactgaa ttagatcgag caattggtga
    14581 tggtgaccac ggggttaaca tggttcgtgg gtttagtagt cttaaagaca aacttgatga
    14641 tagctcaatg caatcattgt tcaaatcgac tggtatggca ttgatgtcaa atgttggggg
    14701 tgcatcagga ccactgtatg gctttagctt tgttaaaatg tctgcagtca ccaaagatga
    14761 tatggataat caagatttca ttacactaat tcaggcattt gccgaagcgg ttgaatcacg
    14821 tggtaaagtt actttaaatg aaaagacaat gtatgatgta gtagcgcgag cagcagagaa
    14881 gcttgaaaat ggtgaaactt taacattcaa tgatttacag caattagcag ataatacaaa
    14941 agatatggta gcaacgaaag gtagagctgc atattttgga gaagaatcaa aaggttatat
    15001 tgatccaggt gctcaaagta tggtttatat tttaaacgct ttgattggag atgaagataa
    15061 tgcctaaaat tatacttgtt agccacagta aagaaattgc aagtggtaca aaatctttgt
    15121 taaagcaaat ggcaggtgac gttgatatta taccaatcgg gggattacca gatggttcaa
    15181 ttggaacttc atttgatatc atccaagaag ttttgactaa attagaggat gatgcattgt
    15241 gtttttacga tattggatct tcagaaatga atgtagatat ggctattgaa atgtatgatg
    15301 gtaatcatcg tgtgttaaaa gttgatgcac caattgttga aggcagtttt atcgcagcag
    15361 taaagctatc aatcggcggt tcaattgatg atgcattagc agaaatcaaa caatcatttt
    15421 agttaaaatt tactaataat gaaaaatgta aacctttttc aaatgaaact ttataaaaaa
    15481 tatgatagta tatatgtaaa tgtttaataa aatctggaga aataggagga cattgccatg
    15541 caacacctta taaaaaaaca tgtattgaat ggcgagtttg atttagtacg acaattgatg
    15601 tccgaaacag attttatgga atttgaagaa gcatatattt caagtgcgca tgaagtagaa
    15661 agtatgatgt tttatacatg tattttagat atgattaagt acgaagaatc atctgaaatg
    15721 catgacttag catttttatt gcttgtgtat ccactaagtg aatatgaagg tgctttggat
    15781 tctgcttatt atcatgcaga cgcttccata aaacttactg acggcaaaga agttaaaagt
    15841 ttgttacaaa tgttattatt gcatgcgata ccaacacctg ttatttcaga taagaaggct
    15901 tttgatatcg ccaagcaaat tttaaaatta gatcctaata ataatgttgc tcgtaacgtc
    15961 ttaaaagaca ctgccaaacg tatggacaac gttgttgttg atataaatga attacaccaa
    16021 cgtaatgcac gttaattaca tttcaattat attagcttaa taatagtttt aacatttggt
    16081 tgggttggga catatgttcc agcctttttt aatacttaaa aactaacgaa gtatacttgt
    16141 gtgcacaaat ggtttttata caacatttta taaatttata cattttaata aagaacatac
    16201 gatagatggt ttaaaccttg ttaactgaga aattttgata tgtattcttc gaaatttaac
    16261 taaatatacg aaattcaaga agcacaataa ttaatcattt ttcctataca aaagttcgta
    16321 tgactgcatt ataaaagcat aaatttataa tttttttaaa tgtcattgaa cgtgataatg
    16381 tgaatggatt gagcaatttt gaaaaagtga aaaataacct atgcgacttg caattaattt
    16441 tcagtacgtt ataatgcaca ctgtgcaaaa ttaaggaggt ctattattca catgatgatg
    16501 aataaagaag caacaaaaat tggatttgcc tacgtcggca ttgtagtggg cgcaggattt
    16561 tcaactggac aagaagttat gcaatttttc actaaatatg gcttgtgggc ttatttaggt
    16621 gttattatat ctggttttat tttagctttt attgggcgcc aagtagcaaa aattggtact
    16681 gcctttgaag cgacaaatca tgaatcaaca ttacaatacg tattcggtga aaagtttagt
    16741 aaagtctttg attatatttt aatcttcttc ttatttggta tagctgtaac catgatagct
    16801 ggtgcaggcg caacatttga agaaagttat aacataccta catggctagg tgctttaatt
    16861 atgacattag cgatttatat tacgttgcta ttagacttta ataaaatagt acgtgcacta
    16921 ggtatcgtta caccattttt aattgtttta gttgtattaa tcgctggcgt ttatttattt
    16981 aaaggtcatg tttcattagc agaagttaac caagtagtgc ctgaagcaag tatttggaag
    17041 ggaatctggt ttggtacaat atatggtgga ttagcttttt ctgtaggttt tagtaccatc
    17101 gtagcaatcg gtggggatac tgaaaagcgt acagtgtcag gtgcaggcgc gatgtatggt
    17161 ggtattatct atactgtatt actagcattg atcaactttg cattgcaaag tgaatatcca
    17221 actattaaaa atgcctcaat tcctacattg acgttagcaa ataatatcca tcctttaata
    17281 gcaacagtgt tatctgttat tatgctggcg gttatgtata atactattct aggactaatg
    17341 tattcatttg cagcacgttt tacagaacca tacagtaaaa attatcatat ctttattatt
    17401 ataatgatgg tagcaggtta tttattaagt ttcgtaggat ttgctgaatt aattaataag
    17461 ttatatacaa ttatgggata tgtaggctta tttattgtag tagctgtaat tattaaatat
    17521 ttcaaacgta aaaatgcgga taaaaaacat attgcttaat atcatatgag ggatatccga
    17581 aactttacaa ttgaatcact ttgttttaac cttaaaagca attcgtctct actcttatcg
    17641 ggcgaattgc tttttatatt tattcagtct attaatatga gcgtctaaca aatagagagg
    17701 tacgatgtaa tgaataaaga taataaatgg acgatgataa ctgcgctttt tataactgta
    17761 atcagtgtat tgttagcatt tcatctgaaa caacattatg accaaattac aaatgagaac
    17821 catgctaata aagacaaaat taatattaaa aataaaaatg tgcgcattta tcaaaacctt
    17881 acatacaata gagttttccc taacagtaaa ttagatatta ttacacctgt tgatatgtct
    17941 tctaatgcca aactgccagt tattttttgg atgcacggtg gtggttatat tgcgggtgat
    18001 aagcagtata aaaacccatt attagcgaaa attgctgaac aagggtacat tgttgtgaat
    18061 gtaaattatg cattggcgcc acaatataaa tatcccacac cattaattca aatgaatcaa
    18121 gcaactcaat tcattaaaga aaataaaatg aatttaccta ttgattttaa tcaagtaatt
    18181 attggcggtg attctgcagg tgctcaatta gctagccaat ttacggcaat acagacgaat
    18241 gatcgcttaa gagaagccat gaaatttgat cagtcattca aaccatcgca aattaaaggt
    18301 gctatactat ttggtggttt ttataatatg caaacagtta gagaaactga gtttccaaga
    18361 atacagttat ttatgaaaag ttatactggc gaagaagatt gggaaaagag ttttaaaaac
    18421 atttcacaaa tgtcgacagt aaaacaatcg acaaaaaatt atccaccaac atttttatct
    18481 gttggagata gcgatccatt cgaaagtcaa aatatagaat tcagtaagaa attacaagaa
    18541 ttgaatgtac cagtagatac tttgttttat gatggtacgc atcatttaca tcatcagtat
    18601 caatttcacc ttaataaacc tgaatcgata gataatatca aaaaagtgtt acttttctta
    18661 agtcgtaata catcttctag tggtattcaa actgaagaga aaccacaaat agaaaatccg
    18721 agtaatgaat taccgttaaa tcctttaaac taatgataaa cagtagtaat ttattactta
    18781 agcaacattt aagatcttca aattgaaaac gaagaattta aaacatgtgg ggctaatgtg
    18841 taagagtctg agatataata aattgttagc ggttctttat catttctatc tcaccctatt
    18901 atgatgtgac atttatttta caaactaatt tgttttgaac ctgaaaataa ctttttacaa
    18961 ctaaattgtc gaaaaacagt gtgtacatga tataataaat ttataaattg aaaagaattc
    19021 aaaagaaaaa ataaaatata gattgagcac gaagtgattt gaaataaggt tgtgaaaggg
    19081 aatgacaagg tcagcattaa aaccatttaa aaataaacgc gttatggtta ctggacgtat
    19141 acaacgtgtt ttgtttaaaa attatttaga tagacatagc acatttaagc cgaatgtaag
    19201 gatattatta aaagatgtat ttgtttcagg tgtatcaata gatcatttat ggttatatga
    19261 gacaaataaa tactatgcat tggcaatgga acttattcat caacgagtaa aatttagtgc
    19321 gaatgttgta ccatattaca aaataaatag aaataataat ttattcgtac aagattatgg
    19381 aattaagcgt aaaggtaggt taattactga agaagcttac aatcaaaaca atcagtatca
    19441 ggataagata tatgaaaaat taccggatat agattttaga ctcgaagatt tttatagtaa
    19501 ggaaaactaa acaacatata taaagcacca ctaatcgtag ttgatagttg atcaattaca
    19561 cattagtggt gctttatttt gaatttaaat ttgataaatg aataagagta tacgcattca
    19621 tttaagatta gatgacattg ataagtatga gtaatgtatt aggtaaccat taaattcgtt
    19681 gttatgcgat tctgatacaa gattatgata aaatagcttt aattaagaat tttagccatc
    19741 atatagtcat cgtaatattt accatcgata aataacttat cttttaaaac gccttcgatt
    19801 tgaaaatcgg cacttttaaa aagctcgagg gcaggttggt tattgagtgg tacatttgct
    19861 tcaattcggt gtatttgatt gtttaaacac caagccataa tggcatcaag aagtgcttgg
    19921 ccaattccac gatgttgata taatttcttt acacctaaat caattttagc aacatgttta
    19981 atgcgttgaa atggtgtcgt attaacaaag gcaaagccaa cgagttgttc atcactttca
    20041 gcaacaaaga tgactttatg tggagaagtg atatattctt ctaattgttt actagccgac
    20101 gtgacgctag gatcatattc tcctggtgtg tagaacatat acggagattc gtcgtatatt
    20161 ttcgctaaca ttgaaatgaa attttctaca tctttgatac taactctacg tataatatgg
    20221 gccataaaaa gcctccagta ttttgaaatt ttaaaacatt tgctactatt ataatatata
    20281 tgtaactaaa aggtggagta atatgttttt tggttacagt ttaaaggagt attttagatg
    20341 aaacctaaag ttttattagc aggtggaaca ggatatattg gtaagtattt aagtgaagtg
    20401 attgaaaatg atgctgaact ttttactata tcaaaatatc cagacaataa aaaaacagat
    20461 gatgttgaaa tgacttggat tcagtgtgat atatttcatt acgaacaggt tgttgcagca
    20521 atgaatcaaa tagatattgc tgtattcttt atcgacccaa caaagaattc tgccaaaata
    20581 acacaatcat cagcaagaga tttaacatta atcgcagcag ataattttgg tcgagcagcg
    20641 gctatcaatc aagtaaaaaa agtaatctac atacctggga gtcgttatga taatgaaaca
    20701 attgaacgcc taggtgcata tggcacacct gtagaaacaa caaatttagt ttttaaacgt
    20761 tctttagtta atgtagaatt acaagtttca aagtatgatg atgttagatc aacgatgaag
    20821 gtagttttac caaagggatg gacattaaag aacgttgtaa accattttat tgcatggatg
    20881 ggttacacta aaggaacttt tgtgaaaaca gaaaaatcac atgatcaatt taagatatat
    20941 attaagaata aggtgcgacc gctcgcagta tttaaaatag aagaaacagc tgacggaata
    21001 ataactttaa ttttattgag tggaagttta gtgaaaaaat acacagttaa tcaagggaag
    21061 ttagaattta gattaatcaa agagtcggca gtcgtttata tacatctata cgattatatc
    21121 cctcgattat tttggccgat ttattacttt atacaagcac caatgcaaaa aatgatgatt
    21181 catggctttg aagttgactg ccggattaaa gattttcaaa gtcgattaaa atcaggagaa
    21241 aatatgaaat atactaaatg atattgggtg atatggatgc aaatactact agtagaagat
    21301 gacaatactt tgtttcaaga attgaaaaaa gaattagaac aatgggattt taatgttgct
    21361 ggtattgaag atttcggcaa agtaatggat acatttgaaa gttttaatcc tgaaattgtt
    21421 atattggatg ttcaattacc taaatatgat gggttttatt ggtgcagaaa aatgagagaa
    21481 gtttccaacg taccaatatt atttttatca tctcgtgata atccaatgga tcaagtgatg
    21541 agtatggaac ttggcgcaga tgattatatg caaaaacctt tctataccaa tgtattaatt
    21601 gctaaattac aagcgattta tcgtcgtgtc tatgagttta cagctgaaga aaaacgtaca
    21661 ttgacttggc aagatgctgt cgttgatcta tcaaaagata gtatacaaaa aggtgacgat
    21721 acgatttttc tatccaaaac agaaatgatt atattagaaa ttcttattac caaaaaaaat
    21781 caaatcgttt cgagagatac aattatcact gcattatggg atgatgaagc atttgttagt
    21841 gataatacgt taacagtaaa tgtgaatcgt ttacgaaaaa aattatctga aattagtatg
    21901 gatagtgcaa tcgaaacaaa agtaggaaaa ggatatatgg ctcatgaata atttgaaatg
    21961 ggtagcttat tttttgaaat ctcgcatgaa ctggatattt tggatattgt ttttaaactt
    22021 gcttatgtta ggcattagtc taatcgatta tgattttcca atagacagtt tattttatat
    22081 tgtttctttg aatttaagtt taacaatgat ttttcttata ttgacatatt ttaaagaagt
    22141 aaaattatat aagcattttg acaaagataa agaaatagaa gaaattaaac ataaagattt
    22201 agcggaaacg ccatttcaac gtcatacagt tgattattta tatcgtcaaa tctcagcgca
    22261 caaagaaaag gttgttgagc aacagttaca attgaacatg catgaacaaa ccattacaga
    22321 atttgtgcac gacataaaaa cacctgtgac agccatgaaa ttattaattg atcaagaaaa
    22381 aaatcaagaa agaaaacagg cattactata tgaatggtct cgtataaact cgatgctgga
    22441 tacacagctg tatattacta gattagaatc tcaacgcaaa gatatgtatt ttgattacgt
    22501 gtcacttaaa cgcatggtca ttgacgaaat acaattaaca agacatatta gtcaggttaa
    22561 aggtattggt tttgatgttg actttaaagt ggatgattat gtttatacag atataaaatg
    22621 gtgtcgtatg attattagac aaattttgtc aaacgcattg aaatatagtg agaattttaa
    22681 tattgaaatt gggacagaat taaatgatca acatgtttcg ttatatatta aagactatgg
    22741 cagaggtatt agtaaaaaag atatgccgcg aatatttgaa cgaggattta cgtcaacggc
    22801 taacagaaat gaaacgacgt cttcaggtat gggtctatat ttagtaaata gtgtaaagga
    22861 tcaattaggt attcacctgc aagtcacgtc gactgttggt aaggggacaa ctgtcagatt
    22921 gattttccca ttacaaaatg aaattgttga acgcatgtcg gaagtgacaa atttgtcatt
    22981 ttaaacatgc gttttgttac ttagaattga tacatcaatg cagcttcaac gttataataa
    23041 gatagatgtt agtcatatgt taaattgaag atacaagtgc caaagcctaa aggaaatgaa
    23101 gttaagataa attataggag tgttaaagtg gcaattttag aagtaaaaca attaacaaaa
    23161 atatatggaa ctaaaaaaat ggcacaagaa gtgttgcgag atatcaatat gtctattgaa
    23221 gaaggcgagt ttattgctat tatgggtccc tctggatctg ggaaaacgac attattaaat
    23281 gttttaagtt caattgatta tatttcacaa ggttctatta cattaaaagg aaaaaaatta
    23341 gaaaagcttt caaacaagga attatctgat atacgcaagc atgatattgg ttttattttt
    23401 caagagtata atttactgca tacattgact gttaaagaaa acataatgtt accactaacg
    23461 gttcagaagt tagataaaga acatatgtta aatcgttatg aaaaagtagc agaagcatta
    23521 aatatattgg atattagtga taaataccct tctgaattgt ctggtggaca aagacaacga
    23581 acatctgctg caagagcgtt tattacatta ccttctatta tatttgctga cgaaccaaca
    23641 ggtgcactgg attctaaaag tactcaagat ttattaaaac gattaacaag aatgaatgaa
    23701 gcatttaagt ctacaattat tatggtaacg catgatcctg ttgcagcaag ttatgccaat
    23761 cgagtagtga tgctaaaaga tggtcaaatt ttcactgaat tataccaagg ggatgacgat
    23821 aaacatacct ttttcaaaga aataatacgt gtacaaagtg ttttaggtgg cgttaattat
    23881 gacctttaac gagataatat ttaaaaattt ccgtcaaaat ttatcacatt atgccatcta
    23941 tcttttttca ttaattacga gtgtagtatt gtattttagc tttgtagcat taaaatacgc
    24001 gcataaacta aacatgacag agtcatatcc aattattaag gaaggctcac aagtcggaag
    24061 ctactttcta tttttcatca taattgcatt tttgttatat gccaatgtgt tatttattaa
    24121 acgacgaagt tatgagcttg cattatatca aacattaggt ttatctaaat tcaacattat
    24181 ttatatacta atgctcgaac aattactaat atttataatt acggcaatat taggtattat
    24241 tattggtatt tttggttcaa aactgttatt aatgattgtc tttacattat taggaattaa
    24301 agaaaaggtt ccaattattt ttagtttgag ggcggtattt gaaacattaa tgttaatcgg
    24361 tgtcgcttat tttttaacct ctgctcaaaa ttttatatta gtgttcaaac aatctatttc
    24421 acagatgtca aagaataacc aggttaaaga aacaaatcat aataaaatta catttgaaga
    24481 ggttgtttta ggcatcttag gtatagtatt gattaccaca ggatactatc tatctttgaa
    24541 cattgttcaa tattatgatt ctatcggtat acttatgttt attttattgt caactgtgat
    24601 tggggcatac ttatttttta aaagctctgt ttctctagtt tttaaaatgg tgaagaagtt
    24661 tagaaaaggt gttataagtg taaatgatgt catgttctca tcatctatta tgtatcgtat
    24721 taagaaaaat gctttttcac ttacggtcat ggcaatcatt tcagcgatta ctgtttcagt
    24781 tctttgcttt gctgctataa gtagagcgtc cttatcaagt gaaataaaat atactgcacc
    24841 acacgacgtt acaattaaag accaacaaaa agctaatcaa ttagcaagtg aattaaacaa
    24901 tcaaaaaatt cctcattttt ataattataa agaagtaatt catacgaaat tgtataaaga
    24961 taatttattt gatgtaaaag cgaaagaacc atacaatgta acaattacta gtgataaata
    25021 tatccctaat actgatttga aacgtggaca agctgatttg tttgtagcgg aaggttctat
    25081 caaagattta gtgaaacata agaagcatgg taaagcagtt ataggaacga aaaaacatca
    25141 tgttaatatt aagttgcgga aagatattaa taaaatctat tttatgacag atgttgattt
    25201 aggtggacca acgtttgtct taaatgacaa agactatcaa gaaataagaa agtatacaaa
    25261 agcaaagcat atcgtctctc aatttggatt cgatttgaaa cataaaaaag atgctttagc
    25321 attagaaaaa gtgaaaaata aagttgataa atctattaaa acaagaagtg aagcgataag
    25381 ctcaatatca agtttaaccg gaatattatt atttgtaaca tcatttttag gtattacatt
    25441 cttgattgct gtatgttgca ttatatacat aaagcaaata gatgaaaccg aagatgagtt
    25501 agagaattat agtattttga gaaagcttgg atttacacaa aaagatatgg caaggggact
    25561 aaagtttaaa attatgttta attttgggtt acctttagtt attgcactat cacatgcata
    25621 ttttacatca ttagcatata tgaaattaat gggtacaacg aatcaaatac cggttttcat
    25681 agtaatggga ttatacattt gtatgtatgc tgtttttgca gtgacggctt ataatcattc
    25741 caagcgaaca attagacatt ccatataaaa tatacagatg gctttcagta gagtagtgga
    25801 ttcggattca cgaactatac tggaagcttt ttattataaa tgaagagaag ttatattttt
    25861 agcatgtata gttgaatact gggttaaaat accatattaa taatgaagta aaggtatgag
    25921 tgattatgaa agtgttttga atgaaatata tttaattggt gatgctttta attgaaaaga
    25981 ttaacaggat tcaactttgt aaattgtatt aaatgtgaga aaataaaagt atattcattg
    26041 agagatatat gagtcaatga tcgttttaaa caagataagt gtatttta
//
LOCUS       DAMZKC010000022        24875 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.022, whole genome shotgun sequence.
ACCESSION   DAMZKC010000022 DAMZKC010000000
VERSION     DAMZKC010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 24875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24875
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.022"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..248
                     /locus_tag="POB55_000649"
     CDS             <1..248
                     /locus_tag="POB55_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499635.1"
                     /translation="HPRKTSQLPLILKTILNLSLIGIVIIVFTHAEFQLNAGDSNVFL
                     LTWDIRGLTSYIFSFLILVALVSKLILIFITRRQRTS"
     gene            complement(308..1906)
                     /locus_tag="POB55_000650"
     CDS             complement(308..1906)
                     /locus_tag="POB55_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HDI6499636.1"
                     /translation="MQKSALFAPEKYNIISEIEKYSHIPDKKAILYHNTEGEDISVTY
                     QQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRT
                     KDLQYRITHGEINAVIALEPFTVEFEKIKEYDALTKFVIAGQKDGWISLEAEKENASN
                     KLEMADTTRDDMAILSYTSGTTGNPKAVTHCHGWGFAHLQMAPKHWLCINEDDLVWAT
                     AAPGWQKWVWSPFLSVLGSGATAFVFNGRFSPETYLELLQKYQINVLCCTPTEYRMMA
                     KLQNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYFNLTVRDGYGQTESTLLIGFLKDT
                     PQRIGSMGKGIPGSSVTVVDDEGNSVPANTKGNIAVPLDLPALFKGYYKEPERTAKAQ
                     TGKYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVA
                     KPHDIRGNIVKAFVILQDHTAGDDTLVKELQQFVKNEVAPYKYPREIEFVDDLPKTNS
                     GKIRRVELRDSEIEKWQQQKDSNQ"
     gene            complement(2100..2543)
                     /locus_tag="POB55_000651"
     CDS             complement(2100..2543)
                     /locus_tag="POB55_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HDI6499637.1"
                     /translation="MNIYDEYRSYVIEELDDCLTIQKNNDTAYYDVLEAINDLSNDSL
                     CVLNHLYINEGQEETFEQKFLRRNKHLKNVDGFKALRFLRPRTAGRHYIIITLWENRQ
                     AFYHWQNSAEYKHTHKHRGTSKGADVKIINRELSYNIRIELADMV"
     gene            complement(2823..3035)
                     /locus_tag="POB55_000652"
     CDS             complement(2823..3035)
                     /locus_tag="POB55_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterile alpha motif-like domain-containing
                     protein"
                     /protein_id="HDI6499638.1"
                     /translation="MSFYEYIQTFKDDKTPLGELAIWIKEDDSFPKQEKLTENILSYF
                     HQMSNIDHEFLEIVKRSLSLYDQLKS"
     gene            complement(3313..5022)
                     /gene="betA"
                     /locus_tag="POB55_000653"
     CDS             complement(3313..5022)
                     /gene="betA"
                     /locus_tag="POB55_000653"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HDI6499639.1"
                     /translation="MSNKNKSYDYVIIGGGSAGSVLGNRLSEDKDKEVLVLEAGRSDY
                     FWDLFIQMPAALMFPSGNKFYDWIYSTDEEPHMGGRKVAHARGKVLGGSSSINGMIYQ
                     RGNPMDYEGWAEPEGMETWDFAHCLPYFKKLEKTYGAAPYDKFRGHDGPIKLKRGPAT
                     NPLFQSFFDAGVEAGYHKTPDVNGFRQEGFGPFDSQVHRGRRMSASRAYLHPAMKRKN
                     LTVETRAFVTEIHYEGRRATGVTYKKNGKLHTIDAKEVILSGGAFNTPQLLQLSGIGD
                     SEFLKSKGIEPRVHLPGVGENFEDHLEVYIQHKCKEPVSLQPSLDIKRMPFIGLQWIF
                     TRTGAAASNHFEGGGFVRSNNEVDYPNLMFHFLPIAVRYDGQKAAVAHGYQVHVGPMY
                     SNSRGSLKIKSKDPFEKPSIRFNYLSTEEDKKEWVEAIRVARNILSQKAMDPFNGGEI
                     SPGPEVQTDEEILDWVRRDGETALHPSCSAKMGPASDPMAVVDPLTMKVHGMENLRVV
                     DASAMPRTTNGNIHAPVLMLAEKAADIIRGRKPLEPQYIDYYKHGVHDENEGAIEVKP
                     YAK"
     gene            complement(5283..6773)
                     /gene="betB"
                     /locus_tag="POB55_000654"
     CDS             complement(5283..6773)
                     /gene="betB"
                     /locus_tag="POB55_000654"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HDI6499640.1"
                     /translation="MELLKHLSQRQYIDGEWVESANKNTRDIINPYNQEVIFTVSEGT
                     KEDAERAILAARRAFESGEWSQETAETRGKKVRAIADKIKEHREALARLETLDTGKTL
                     EESYADMDDIHNVFMYFAGLADKDGGEMIDSPIPDTESKIVKEPVGVVTQITPWNYPL
                     LQASWKIAPALATGCSLVMKPSEITPLTTIRVFELMEEVGFPKGTINLILGAGSEVGD
                     VMSGHKEVDLVSFTGGIETGKHIMKNAANNVTNIALELGGKNPNIIFDDADFELAVDQ
                     ALNGGYFHAGQVCSAGSRILVQNSIKDKFEQALIDRVKKIKLGNGFDADTEMGPVIST
                     EHRNKIESYMDVAKAEGATIAVGGKRPDRDDLKDGLFFEPTVITNCDTSMRIVQEEVF
                     GPVVTVEGFETEQEAIQLANDSIYGLAGAVFSKDIGKAQRVANKLKLGTVWINDFHPY
                     FAQAPWGGYKQSGIGRELGKEGLEEYLVSKHILTNTNPQLVNWFSK"
     gene            6842..6937
                     /locus_tag="POB55_000655"
     CDS             6842..6937
                     /locus_tag="POB55_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499641.1"
                     /translation="MKNVSIYKTTQELSFVSLLDFYLTKHLTLML"
     gene            7026..7589
                     /locus_tag="POB55_000656"
     CDS             7026..7589
                     /locus_tag="POB55_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GbsR/MarR family transcriptional regulator"
                     /protein_id="HDI6499642.1"
                     /translation="MTRSQNKQQQLEQAKDIVINSIGQTMDLYGTNRSVGNLYGTMVF
                     EGSMTLDEMRHQLQMSKPSMSAGVKKLQEFDIVKQQFTRGSRKQHFIAEKDFFIFFRN
                     FFTKKFQREIDINVEAVKDAQAIINPLLESSDLTEAETQEAQKIKAQLDHTHVYYEWL
                     EQLTEAIESGEIFKYFPIPEQPSDSEN"
     gene            complement(7777..8463)
                     /locus_tag="POB55_000657"
     CDS             complement(7777..8463)
                     /locus_tag="POB55_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDI6499643.1"
                     /translation="MTNQFKNKQSKLHDSLESITKNLYATPTSELPFDNRFLFKSFIL
                     KRETGNIVIYHSGHLGDSQQDIASLGGVSKVLMNHDHESIGGSNQVEAPYFIHENDVA
                     ALKHKISVQKQFSNRVMLDKDLEVIPAPGHTPGTTLFLWDDGHHRYLFTGDFICFEGK
                     RWRTVILGSSDREKSIQSLEMVKELDFDVLVPWVTIKDEPLVYFVENEYEKREQIQNI
                     IDRVREGENS"
     gene            complement(8722..10344)
                     /locus_tag="POB55_000658"
     CDS             complement(8722..10344)
                     /locus_tag="POB55_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HDI6499644.1"
                     /translation="MKKEKVMDWTTFIGTVAVLLFAVIPMMAFPKASEDIITGINSAI
                     SDSIGSIYLFMGLAIFCFVMYIAFGKYGNVTLGKASDKPEFNTFTWAAMLFCAGIGSD
                     ILYWGVIEWAFYYQVPPNGAKSMSDEALQYATQYGMFHWGPIAWAIYVLPALPIGYLV
                     FVKKQPVYKISQACRPILKGQTDKFVGKVVDILFIFGLLGGAATSLALGVPLISAGIE
                     RLTGLDGKNMILRSAILLTITVIFAISSYTGLKKGIQKLSDINVWLSFVLLAFIFIIG
                     PTVFIMETTVTGFGNMLRDFFHMATWLEPFGGIKGRKETNFPQDWTIFYWSWWLVYAP
                     FIGLFIARISKGRRLKEVVLGTIIYGTLGCVLFFGIFGNYAVYLQISGQFNVTQYLNT
                     HGTEATIIEVVHHLPFPSLMIVLFLVSAFLFLATTFDSGSYILAAASQKKVVGEPLRA
                     NRLFWAFALCLLPFSLMLVGGERALEVLKTASILASVPLIVIFIFMMISFLIILGRDR
                     IKLETRAEKLKEVERRSLRIVQVSEEEQDDNL"
     gene            10476..10637
                     /locus_tag="POB55_000659"
     CDS             10476..10637
                     /locus_tag="POB55_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499645.1"
                     /translation="MAIIEYLYNFSRNKQNLNSAKTIVLSYDLTNLTNFTRTAFKNIY
                     TLLTNIYLL"
     gene            complement(10653..10763)
                     /locus_tag="POB55_000660"
     CDS             complement(10653..10763)
                     /locus_tag="POB55_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499646.1"
                     /translation="MIEYDLTIILIPILKVAYPFFKKVEREKWYFSGKEV"
     gene            complement(10863..11399)
                     /gene="nrdG"
                     /locus_tag="POB55_000661"
     CDS             complement(10863..11399)
                     /gene="nrdG"
                     /locus_tag="POB55_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HDI6499647.1"
                     /translation="MTLLDIKQGQGYIAKIESNSFVDGEGVRCSVYVSGCPFNCVGCY
                     NKASQKFRYGEKYTDEILAEILDDCDHDYISGLSLLGGEPFCNLDITLNLVKAFRARF
                     GNKKTIWVWTGFLYEYLANDCAERRELLSYIDVLVDGLFIQHLFKPDLPYKGSLNQRI
                     IDVQQSLSHARMIEYIVS"
     gene            complement(11396..13246)
                     /gene="nrdD"
                     /locus_tag="POB55_000662"
     CDS             complement(11396..13246)
                     /gene="nrdD"
                     /locus_tag="POB55_000662"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HDI6499648.1"
                     /translation="MNQIEEALTGLISKDPAIVNENANKDSDTFSTMRDLTAGIVSKS
                     YALNYLLPKHVADAHQRGDIHFHDLDYHPFQPLTNCCLIDAKNMLHNGFEIGNANVTS
                     PKSIQTASAQLVQIIANVSSSQYGGCTVDRVDELLSTYARHNEEQHRNIAKQFVKESE
                     IDRYVDQQVTKDINDAIESLEYEINTLYTSNGQTPFVTLGFGLGTDHLSRKIQQAILN
                     TRIKGLGKDRTTAIFPKLVFSIKKGTNFSPQDPNYDIKQLALKCSTKRMYPDILNYDK
                     LVEILGDFKAPMGCRSFLPSWKDAEGHFENNGRCNLGVVTLNLPRMALESAGNMTKFW
                     EIFYERIDVLHDALLYRINRLKDAVPNNAPILYKSGAFNYKLKETDDVAELFKNKRAT
                     ISMGYIGLYETATVFYGPDWETSQEAKAFTLEILKEMKRYQTKWTELYDIWFSIYSTP
                     SESLTDRFCRLDQERFGDIKDITDKGYYQNSFHYDVRKDVTPFEKLDFEKDYPYYASG
                     GFIHYCEYPKLQHNLKALEAVWDYSYDKVGYLGTNIPIDHCYECDYDGDFEATEKGFK
                     CPNCGNDNPKTVDVVKRTCGYLGNPVQRPVIKGRHKEICARVKHMKAPKE"
     gene            complement(13492..14862)
                     /locus_tag="POB55_000663"
     CDS             complement(13492..14862)
                     /locus_tag="POB55_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HDI6499649.1"
                     /translation="MNSDNMWLTVMGLIIIISIVGLLIAKKINPVVGMTIIPCLGAMI
                     LGYSVTDLVGFFAKGLDQVINVVIMFIFAIIFFGIMNDSGLFKPLVKRLIIMTRGNVV
                     IVCAMTALIGTIAQLDGAGAVTFLLSIPALLPLYKALNMNKYLLILLLALSAAIMNMV
                     PWGGPMARVAAVLKAKSVNELWYGLIPIQIIGFILIMLFAVYLGFKEQKRIKKAIERN
                     ELPQTQDIDVHKLVEVYERDQDVRFPVKGRARTKSWIKWVNTALTLAVILSMLINIAP
                     PEFAFMIGVSLALVINFKSVDEQMERLRAHAPNALMMAAVIIAAGMFLGVLNETGMLK
                     AIATNLIKVIPAEVGPYLHIIVGLLGVPLDLLTSTDAYYFAVLPIVEQTAGQFGVPSV
                     STAYSMVIGNIIGTFVSPFSPALWLAIGLAEANMGTYIKYAFFWIWGFAIVMLVIAML
                     MGIVTI"
     gene            complement(15338..15943)
                     /locus_tag="POB55_000664"
     CDS             complement(15338..15943)
                     /locus_tag="POB55_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HDI6499650.1"
                     /translation="MNMPLMIDLTNKNVVIVGGGLVASRRAQTLSQYVEHIMVISPEI
                     TEKLQNMVDKGVVIWKEKEFEPSDIVDAYLVIAATNEPRVNEAVKQALPEHALFNNVG
                     DASNGNVVFPSALHRDKLTISVSTDGASPKLTKSIMAELEALYPPSYSSYIDFLYTCR
                     QKIKLLDMNHSEKQQLLSQIVTKEFINDTKQAQFLAWLDKK"
     gene            complement(16006..17886)
                     /locus_tag="POB55_000665"
     CDS             complement(16006..17886)
                     /locus_tag="POB55_000665"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
                     /protein_id="HDI6499651.1"
                     /translation="MKLNTSNSPFTEKQVTEINNLLQTLTESQQQWLSGFLLANSNDT
                     TSDSNQQQLETEVWQQSQISEEQATSTTYMLQNKEPHIEANQRHVTVLYGSESGNAMR
                     LAEIFSERLSDIGHQVVLMSMDEYDTTNIAQLEDLFIITSTHGEGEPPDNAWDFFEFL
                     EDDNAPNLNHVRYSVLALGDQTYEFFCQAGKDVDALLENLGAERICKRVDCDIDYEED
                     AEKWMADIINMIDTTSEGIQSESVISESIKSAKEKKYSKLNPYQAEVLANINLNGTDS
                     NKETRHIEFLLDDFSESYEPGDCIVALPQNDPELVEKLISMLGWDPQSPVPINDHGDT
                     VPIVEALTSHFEFTKLTLPLLKNADIYFDNEELSERIQDESWAREYVINRDFIDLITD
                     FPTIELQPENMYQILRKLPPREYSISSSFMATPDEVHITVGTVRYQAHGRERKGVCSV
                     HFAERIKPGDIVPIYLKKNPNFKFPMKQDIPVIMIGPGTGIAPFRAYLQEREELGMTG
                     KTWLFFGDQHRSSDFLYEEEIEEWLENGNLTRVDLAFSRDQEHKEYVQHRIMEESERF
                     NEWIEQGAAIYICGDEKCMAKDVHQAIKDVLVKERHISQEEAETILRQMKQQQRYQRD
                     VY"
     gene            18080..18172
                     /locus_tag="POB55_000666"
     CDS             18080..18172
                     /locus_tag="POB55_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499652.1"
                     /translation="MSVINAYSNASLLFFIYLLFIMYQISLSVK"
     gene            complement(18280..18777)
                     /locus_tag="POB55_000667"
     CDS             complement(18280..18777)
                     /locus_tag="POB55_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HDI6499653.1"
                     /translation="MNNRNVYDIEVSDYKGLTYKLEAFRGKVILVVNTATECIYSEQL
                     KKLETLFQKYKDRGFVVLSFPNNNFDNRQPGSNEEILKISREKFGVTFPVLAKISVNG
                     NDEHPLFTHLKNEQPGIFGSPIKWNFTKFIIDRQGNVVNRFLPMEDPLDISTNIEILL
                     QESSS"
     gene            complement(18787..19047)
                     /locus_tag="POB55_000668"
     CDS             complement(18787..19047)
                     /locus_tag="POB55_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499654.1"
                     /translation="MIIMRGYNERYCSWTNTAIQCDYFNKVRQVKKDGKSDRLSNNIN
                     SNIIYRVLEKCITLYNVCSRCKRALILSEHSKKNIFNIKKAV"
     gene            complement(19429..19542)
                     /locus_tag="POB55_000669"
                     /pseudo
     CDS             complement(19429..19542)
                     /locus_tag="POB55_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501402.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(20201..22201)
                     /locus_tag="POB55_000670"
     CDS             complement(20201..22201)
                     /locus_tag="POB55_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6499655.1"
                     /translation="MKLRLLWHIVRRQFITQRLVIIPFILAVSVLFMIEYTLVSIGLN
                     SYIKQKNDFLVPFIIIANFFMALLTFIFIFYANHFMMSQRRKEFSIFMTLGMTKKSMR
                     LIVVMETILQFIIISVVSIAGGYLLGAIFFLFIQKIMGSEVATLRYYPFDFVAMFITL
                     IIIAVLMGMLLIFNLFSINFQRPITYQHRSDSSVISRWLRYVLIVIGSAALYLGYFIA
                     LQQDTTFGAFFKIWIVIGLVIIGTYAFFVGISEIIISILQQVSKVYYHPRYFFVVVGM
                     RVRLKMNAVSLATITLLCTFLIVTLTMTLTTYRDMNHTITKLITNDYDLSFSDNSKSQ
                     IERQQTIENIKRDIQGSVNAKDFKVYETTLFRASLEKNRAYYKLKQASDDIPIDFIGN
                     EGAIFSRQSVMITAFTAQDYNRYHQNKLHLDNQSIGMITNVPIFKKQSKVGLNNEVFK
                     VKQLDAHALNLMMIQDSMVLIVKDNNQLQKVKGFYAHEQKNSTISINFNVFGKTKLTT
                     SQIQKLSKKYDASINSKSEMLMVWDRLTGGLIFVGGVVSIVLVIGIFLMMYYKQVSEA
                     YANQHNYDIMKKLGLDNGRIAKITRNQMTFLFAIPITVALIHTLISSNIVYTLLNMIG
                     INNHHIFLTSYVLAVIIISFLYMAMYKITSYIYAKVIHQQRN"
     gene            complement(22198..22953)
                     /locus_tag="POB55_000671"
     CDS             complement(22198..22953)
                     /locus_tag="POB55_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6499656.1"
                     /translation="MLLEVKHVKKVYGKGLNATTALNQMNLSVGAGEFVAIMGESGSG
                     KSTLLNLIASFDGLTEGDIIVDGAHLNNMKNKSKALYRQQMVGFVFQDFNLLPTMTNK
                     ENIMMPLILAGAKRKDIEQRVHQLAVQLHLEGFLNKYPSEISGGQKQRIAIARALVTK
                     PTILLADEPTGALDSKTSKALMMLFQEIHQLEQTILMVTHSNIDASYAERVIFIKDGR
                     LYHEIYRGEESQLAFQQRITDSLALVNGGSVNI"
     gene            complement(23061..23948)
                     /gene="nsaS"
                     /locus_tag="POB55_000672"
     CDS             complement(23061..23948)
                     /gene="nsaS"
                     /locus_tag="POB55_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nisin susceptibility-associated two-component
                     system sensor histidine kinase NsaS"
                     /protein_id="HDI6499657.1"
                     /translation="MTFLKSITQEIAIVIVIFALFGLMFYLYHLPLEAYLLALGVILL
                     LLLIFIGIKYLSFVKTISQQQQIENLENALYQLKNEQIEYKNDVESYFLTWVHQMKTP
                     ITAAQLLLERDEPNVVNRVRQEVIQIDNYTSLALSYLKLLNETSDISVTKISINNIIR
                     PIIMKYSIQFIDQKTKIHYEPCHHEVLTDVRWTSLLIEQLINNALKYARGKDIWIEFD
                     EQSNQLYVKDNGIGISEADLPKIFDKGYSGYNGQRQSNSSGIGLFIVKQISTHTNHPV
                     SVVSKQNEGTTFTIQFPDE"
     gene            complement(23959..24624)
                     /gene="nsaR"
                     /locus_tag="POB55_000673"
     CDS             complement(23959..24624)
                     /gene="nsaR"
                     /locus_tag="POB55_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nisin susceptibility-associated two-component
                     system response regulator NsaR"
                     /protein_id="HDI6499658.1"
                     /translation="MKILIVEDDFVIAESLASELKKWNYGVIVVEQFDDILSIFNQHQ
                     PQLVLLDINLPTLNGFHWCQEIRKTSNVPIIFISSRIDNMDQIMAIQMGGDDFIEKPF
                     NLSLTIAKIQALLRRTYDLSVANDSLTVKGCTLILDEAKVVYQEQNIQLSLTELQILK
                     LLFQNEDKYVSRTALIEKCWESENFIDDNTLAVNMTRLRKKLNTIGVNDFIITKKNVG
                     YKV"
     gene            complement(24650..24850)
                     /locus_tag="POB55_000674"
     CDS             complement(24650..24850)
                     /locus_tag="POB55_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499659.1"
                     /translation="MILVMLSPLLIIFFIVLSILEERKRTKKKQLEKEKANTLNQNTN
                     DTESSNQEPSLQQAKEQKDNKG"
BASE COUNT         7820 a         4671 c         3561 g         8823 t
ORIGIN      
        1 tgcatccaag aaaaacatca caattacctt taatattgaa aactattttg aatctttcac
       61 ttataggcat tgtgattatc gtttttacac atgctgagtt tcaacttaat gccggtgata
      121 gtaatgtgtt tttattaaca tgggatattc gaggtttaac atcttatatt ttctcattct
      181 taatactggt tgcgcttgtt tctaaactaa ttttaatatt tataactaga cgacaaagaa
      241 catcataaat aaacaagcag tccagtgcgt atcatcatac cttacgcatt ggactgcttt
      301 gctatgttta ttgattgcta tctttttgtt gttgccattt ttctatttca gaatctcgta
      361 gttcaacacg acgaatttta cctgagtttg tttttggtaa gtcgtcaacg aattcaatct
      421 ctctcggata tttatatggt gcaacttcat ttttaacaaa ttgttgtagt tctttaacta
      481 acgtatcatc acccgcagta tggtcctgta aaataacgaa tgctttaaca atatttcctc
      541 gtatatcatg aggctttgct actacagcac attctttaac ggctggatga ttcgttaacg
      601 catcttcaac ttcaaaaggc ccaatcgtat agcctgaact aataataatg tcatctcgac
      661 gtccttcaaa ccagaaataa ccatcatcat ctacatgagc taagtcacca gtgatgtagt
      721 atttacctgt ttgcgctttc gccgtacgtt ctggctcttt ataataccct ttaaaaagtg
      781 ctggcaaatc aagtggtact gcaatattcc ctttcgtatt agcaggtacg ctattcccct
      841 catcatctac tacagtgacc gaactacctg gaatgccttt acccattgat ccaatcctct
      901 gtggtgtatc ttttaaaaag cctataagca aggtactttc agtctggcca tatccatctc
      961 ttacagttaa attaaagtat ttcttgaatt gttcaactac ttctcgattt agtggctcac
     1021 ctgcagaaac ggcactatgt aaatgcgtta agtcataatc atttaagttc tgtaatttag
     1081 ccatcatacg atattctgtc ggtgtacaac ataaaacatt aatttgatat ttttgaagca
     1141 attctaagta tgtttcagga ctgaatcttc cattaaatac aaaagcagtt gcgcctgaac
     1201 ctaatacaga taagaaagga ctccataccc acttttgcca acctggtgct gctgttgccc
     1261 aaactaagtc atcttcatta atacataacc aatgttttgg tgccatttgt aaatgtgcaa
     1321 atccccatcc atgacaatgt gtaacggctt taggattgcc agttgtacca gatgtatatg
     1381 acagaatcgc catatcatca cgcgtcgtat ctgccatttc tagtttgtta cttgcgtttt
     1441 ctttttcagc ttcaagtgaa atccatccat ctttttgacc ggcaataaca aatttagtta
     1501 acgcatcata ttctttaatt ttttcaaatt caactgtgaa tggctctagt gcaataactg
     1561 catttatttc accgtgcgtg atacggtatt gtaaatcttt agttcttagc atttcagaac
     1621 atggaatgat tgcaacacct aattttaaag cagcaatata taattcatac gtcgcaatag
     1681 atcgtggcat cataatgagt actttatcgc ctttagataa accgtgcgat gctaaaacat
     1741 tacctacttt attagactgt tcaatgagct gttggtaagt gactgatata tcttcgcctt
     1801 cagtattatg atataaaatt gcctttttat ctggtatgtg gctatatttt tcgatttccg
     1861 aaataatgtt atatttttca ggcgcgaata gagctgactt ttgcataact aacttccttt
     1921 catacatcca cttttcctgt gatgaacatt gtaattttat aaatgaatta tatatatcat
     1981 acgcctatct ttacagaatt ttcaattaaa tagggttaaa taccaaagtc ctcgacacta
     2041 cactttgaca tgacgtagca ttcaaggact ttcaaatgat tgagggttga tatctcgggc
     2101 tagaccatat cagctaattc aatacgaata ttgtatgata attcacgatt aattattttt
     2161 acatctgcac ctttcgaagt gccacgatgc ttgtgtgtat gcttgtactc agctgaattt
     2221 tgccaatgat aaaatgcttg cctattttcc cacagcgtaa taatgatata gtgtctacca
     2281 gctgttctag gtcttaaaaa ccttaatgct ttaaatccat caacgttttt taaatgctta
     2341 ttacgtcgca aaaatttttg ctcgaatgtt tcttcttgtc cttcgttaat atataaatga
     2401 tttaacacac ataatgaatc atttgataaa tcatttatcg cttccaaaac atcgtaatat
     2461 gcagtgtcat tatttttttg tattgtgaga caatcatcca gttcttctat tacataactt
     2521 ctatattcat cataaatgtt cataataaat gccttcattt catttatatt tttggtcata
     2581 ttactttata tctattacta attcattccc gtatttatta attacaatca tagtttggct
     2641 tctttttaaa agataagact ttgtaaaaag tattaatatt tcatgcaaat ggggacagga
     2701 gtcgcccact atttttgtgt cttcaatttc atgatcatta tttaacatta gtcatgaaaa
     2761 tagccgacaa cttcatctgt gaaatcaccg gccttttatt ttagctaact ttatttctga
     2821 ttttacgatt ttaattgatc atacagagaa agtgatcttt ttacaatttc taaaaactca
     2881 tgatctatat tggacatttg atgaaaataa gacaaaatgt tttctgttag cttctcttgt
     2941 tttgggaatg aatcatcttc tttaatccaa atcgctaatt cgcctaatgg tgttttatca
     3001 tctttaaatg tttgtatata ttcgtaaaag ctcatagtat tccttctctc aatttactta
     3061 tataaatcct accacgaaag ctttcaagaa aacacaatta aatgtctatt tagtgaactt
     3121 tttaaggttg tgcactcttt taatgtctgc caattaggtc aattaatcat cacaatgtac
     3181 aattaactct attttcagtt catatactca cacaccgttt ttgaacaaca cattaacttc
     3241 tcatttagat aaaacgcaaa aaagcctggc accaatacaa tagatgccag actaagagcc
     3301 tactatataa atttatttag cgtatggttt tacttcgatt gcaccttcat tttcatcatg
     3361 aacaccatgc ttataataat caatatattg tggctctaaa ggctttctgc cacgtataat
     3421 gtctgctgct ttttcagcta acattaaaac aggtgcgtgt atattgccat ttgtcgtacg
     3481 tggcatagcg gatgcatcaa ctacacgtaa attttccata ccgtggactt tcattgttaa
     3541 cgggtcaact actgccattg gatctgaagc aggacccatt ttagcactac aagatgggtg
     3601 taatgctgtt tcaccatctc tacgaaccca atcaagaatt tcttcgtctg tttgcacttc
     3661 tggtcctggt gaaatttctc caccattgaa tggatccatt gctttttgag ataagatatt
     3721 tcttgctaca cgaattgctt ctacccattc ttttttatct tcttctgttg ataaataatt
     3781 aaagcggata cttggttttt cgaatggatc tttagatttg attttcaagc taccacgaga
     3841 gtttgaatac attggtccta cgtgaacttg ataaccatgt gcgaccgctg ccttttgacc
     3901 atcatatctt acagctattg gtaagaaatg gaacattaag ttaggataat caacttcgtt
     3961 atttgaacgt acaaatccgc caccttcaaa atggttagat gctgctgcac ctgtacgtgt
     4021 gaaaatccat tgtaaaccaa taaatggcat gcgcttgata tctaagcttg gctgtaatga
     4081 tacaggttcc ttacatttat gttgaatgta tacctctaag tgatcttcaa agttttcacc
     4141 cacaccaggt aaatgaacac gtggctcaat gccttttgat tttaggaact ctgaatcacc
     4201 tataccagat aattgtagta attgtggcgt attgaatgcc ccaccagaca aaatgacttc
     4261 tttagcatcg atggtatgta gtttgccatt tttcttatac gtaacaccag ttgctcttct
     4321 accttcataa tgaatttcag ttacaaaggc acgtgtttca acggttaagt ttttacgctt
     4381 catcgctgga tgtaaatatg ctcttgaagc tgacattcgg cgaccacgat gtacttgact
     4441 atcgaacggt ccaaaacctt cttgtctaaa tccattcaca tcaggtgttt tatgatagcc
     4501 tgcttcaaca cctgcatcaa agaatgactg gaataaagga ttcgttgctg gccctcgttt
     4561 taacttaatt ggtccatcat ggcctctaaa tttatcataa ggcgctgcac cgtatgtttt
     4621 ttctaatttt ttaaaatacg gtaaacagtg tgcaaaatcc caagtttcca taccttctgg
     4681 ttctgcccag ccttcatagt ccattggatt accacgttgg taaatcatgc cattaatcga
     4741 acttgatcca cctaaaactt tacctcgggc atgtgctact ttacgaccgc ccatatgtgg
     4801 ttcttcatct gttgaataaa tccaatcgta aaatttattg cctgaaggga acattaacgc
     4861 agcaggcatt tggataaata aatcccaaaa ataatcactg cgacccgctt ctaatactaa
     4921 gacttcttta tctttatctt cactcagacg attacctagt acagaacctg cactgcctcc
     4981 tccaatgatg acataatcat atgatttgtt tttgttactc atcgttatga cctccaaagt
     5041 ttgttgtatt tcaaagtgag tattgcatga aatatttgtt agtggctgat tggttatcgt
     5101 tgaattgcta aacttagcta accaaaacac tcgattttcc ttttacgatt gttactaaca
     5161 gtcatgaaat aatctatgat tcattttgcg tttagtccaa attatcaatc aacatttcgc
     5221 cacttataca aaacaaagtg agtgtcttgt gttcttacaa tggcactcac cttatctaat
     5281 ttttatttgc taaaccaatt cactaattgt ggatttgtat ttgttaaaat gtgttttgaa
     5341 acaaggtact cttctaagcc ttctttgcct aattctctac cgatacctga ttgtttgtat
     5401 ccaccccatg gcgcttgtgc aaaatatgga tggaaatcat taatccacac cgttccaagt
     5461 ttcaacttgt tagcaacgcg ttgcgctttt ccaatatctt tagaaaatac agcacctgct
     5521 aaaccatata tagaatcatt cgctaattga atcgcttctt gttcagtttc aaagccttct
     5581 acagtaacga caggtccgaa aacctcttct tgtacaatac gcattgacgt atcacaattt
     5641 gtaatgactg ttggctcgaa gaatagacca tcttttaaat cgtctctatc tggacgttta
     5701 ccaccaacag caattgttgc gccttctgct ttagctacat ccatataaga ttcgatctta
     5761 ttacgatgtt ctgttgaaat cactggtccc atttcagtat cagcatcaaa accattacct
     5821 aatttgattt ttttcacgcg atcaataagt gcttgctcaa atttgtcttt aatactgttt
     5881 tgtactaata ttcttgatcc tgctgaacaa acttgacctg catggaaata tccaccattt
     5941 aacgcttggt ctactgccaa ttcaaaatca gcatcatcaa agataatgtt tggattttta
     6001 ccgccaagtt ccaaggcaat attcgtaaca ttattagcag catttttcat aatatgctta
     6061 ccagtctcaa tgccacctgt aaatgataca aggtcaacct ctttatgacc tgacattacg
     6121 tcaccaactt cagaacctgc acctagaata agattaattg ttcctttagg gaaaccaact
     6181 tcttccatta attcaaaaac acgtattgtt gttaatggtg taatttcact tggtttcata
     6241 actagtgaac aacccgtagc aagcgctggc gcaattttcc atgatgcttg taataacgga
     6301 taattccaag gtgtaatttg tgtaactaca cctactggtt ctttaacaat tttgctttct
     6361 gtatctggaa ttggtgaatc aatcatttcg ccaccgtctt tatctgctaa tccagcaaaa
     6421 tacataaaca cattatgaat atcatccata tctgcatatg attcttctaa cgtttttcca
     6481 gtatctaatg tttctaatcg tgctaacgct tcgcgatgtt ctttaatctt gtccgcgatg
     6541 gcacgtactt ttttacctct tgtttcagca gtttcttgcg accattcacc agactcaaac
     6601 gcacgtcttg cagctaagat tgcacgttct gcatcctctt ttgtcccttc agaaaccgta
     6661 aatatcactt cttgattgta aggattgata atatctcttg tatttttatt cgcgctttca
     6721 acccactcac catcaatata ttgacgctga gataaatgtt ttaaaagttc cattcaacaa
     6781 cctccgttaa gtttgttaaa tttttattta acgaatttta caaacttatg ttagcataga
     6841 aatgaaaaat gtgtcaattt acaaaacaac tcaagaatta agttttgtaa gtttgttaga
     6901 tttttattta acgaaacatt taactttaat gctttaaata tttttaatgt ctttttttag
     6961 aaaaggtttt ataatgtatt agtatttatt ttagaatgac caaaatgatg tacggaggtg
     7021 caatgatgac tcgttctcag aacaaacagc aacaattaga acaagcaaaa gatattgtaa
     7081 ttaattcaat tggacagaca atggatttgt atggcactaa tagaagtgta ggaaacttat
     7141 atggcactat ggtgtttgaa ggcagtatga cgcttgatga aatgcgtcat caattgcaaa
     7201 tgagcaaacc aagtatgagt gctggcgtta agaaattgca ggaatttgat attgttaaac
     7261 aacaatttac gagaggaagc cgcaaacaac attttatcgc cgagaaagac tttttcatct
     7321 ttttccgaaa ctttttcact aaaaaatttc agcgtgaaat tgacatcaat gtcgaagcag
     7381 taaaagatgc tcaagctatt atcaatccat tacttgaaag cagtgattta actgaagctg
     7441 aaacacaaga ggcacaaaaa attaaagctc agcttgatca tacacatgtt tattacgaat
     7501 ggttagaaca gttaaccgaa gcgattgaaa gcggtgaaat tttcaaatat ttcccaattc
     7561 cagaacagcc ttctgattct gaaaattaaa attgactcaa acaacgacaa tgatgattat
     7621 gcatgatgaa aacaatgcaa taggtccacg aaataaacgg taatttctat ctcaataaat
     7681 agaaattacc gtttttgttt tatatgacaa tcaatcatgg aggtattttc tagagctttc
     7741 ttagattcgt tacattgctt cgccaattta tttcaattag ctattctcgc cctcacgtac
     7801 tctatcaata atattttgta tttgttcacg tttttcatat tcattttcta caaaataaac
     7861 taacggttca tctttgatag taacccaagg tacaagtaca tcaaaatcta attctttaac
     7921 catctctaaa ctttgaatag atttttctct atcacttgaa cctaatataa ctgtacgcca
     7981 tctcttccct tcaaaacata taaaatctcc agtaaataag taacggtgat gaccatcatc
     8041 ccataaaaat agtgtcgtcc ctggtgtatg tccaggcgcg ggaataactt ctaaatcctt
     8101 atccaacatt acacgattac taaattgttt ttgaacagaa atcttatgtt tcagtgcagc
     8161 cacatcattt tcatgtataa agtaaggggc ttcaacttga ttagaacctc ctatagattc
     8221 atgatcatga ttcatcaata cttttgaaac accacctaat gatgcaatgt cttgttggga
     8281 gtcacctaag tgtccggaat gatagataac aatattacct gtttcacgtt ttaaaataaa
     8341 agatttaaat agaaatcgat tatcaaaagg cagttccgaa gtaggtgtcg catataagtt
     8401 ttttgtgatg gattctaaac tgtcatgtaa tttggactgt ttatttttaa attgattagt
     8461 cattttattt catccttcga ttaatttgaa ttgtacatac gcagtaccct ttatttaata
     8521 atcaatgaga gtctttcaaa cttcgattta gaatatgctt catacaataa ataagtactt
     8581 ggagcactaa aaaattaaat cttttagtac aacattaatg aaaacctaaa agttcatcct
     8641 acaatgctac taaaaaaagg ggaatggaac agaaatgata ttttcacaaa attcatttcg
     8701 tcgtcccaga cccgctttga attataaatt atcgtcttgt tcttcttctg atacttgaac
     8761 gattcgcaat gaacgacgtt caacttcttt taatttttca gcacgcgttt caagtttaat
     8821 tctatcgcgc cctaaaatga ttaaaaatga tatcatcatg aaaataaaaa taacaattaa
     8881 tggcacactt gccagtattg aagcagtttt caatacttct aatgcacgtt cgccaccaac
     8941 tagcatcaat gaaaatggca ataagcacaa tgcaaatgcc cagaataaac gattggcacg
     9001 taatggttcg cctaccactt ttttctgaga tgctgccgct aaaatatatg aacccgaatc
     9061 aaatgttgtt gctaagaata agaaagcaga tactaagaat agtacaatca tcaatgatgg
     9121 gaatggtaaa tgatgcacca cttcaataat ggttgcctct gtaccatgtg tatttaaata
     9181 ttgtgttaca ttaaactgtc cagaaatttg caaatacaca gcatagttac caaaaatacc
     9241 aaagaataat acgcatccaa gcgttccata aataattgtt cctagcacga cttctttaag
     9301 gcgtcgacct tttgaaattc tagcgataaa taaaccgata aatggcgcat atactaacca
     9361 ccatgaccag tagaatattg tccagtcttg tgggaaattc gtttcttttc gacctttaat
     9421 accaccgaat ggttctaacc atgttgccat atggaaaaaa tctctcaaca tatttccgaa
     9481 ccctgtcact gtcgtttcca taataaaaac agtcggtcca ataataaata taaaggctaa
     9541 aagtacaaag gatagccaaa cgttgatatc acttaacttt tgaatacctt ttttcaatcc
     9601 tgtatatgaa ctaatggcaa atataaccgt gattgttaat aaaatggccg aacgtaaaat
     9661 catattttta ccatctaaac cagttaatct ttctatgcct gcagaaatta atggcacacc
     9721 taacgctagt gatgttgccg caccacctag caatccaaag ataaataaga tatctacaac
     9781 tttacctaca aatttatctg tttgaccttt taaaatcgga cgacaagctt gactaatttt
     9841 atacaccggt tgttttttaa caaatactaa ataaccaatt ggtaatgctg gtaaaacgta
     9901 aatagcccaa gcaattggcc cccagtggaa cataccatat tgcgtcgcat attggagtgc
     9961 ttcatcactc atacttttcg cgccatttgg tggaacttga tagtaaaaag cccattcaat
    10021 aacgccccag tataaaatat cagagcctat gcctgcacaa aacagcattg ccgcccatgt
    10081 aaatgtatta aattctggtt tatcacttgc tttaccaagt gtgacattac catatttacc
    10141 aaatgcgata tacattacaa agcaaaaaat cgccagcccc ataaataaat atatcgaacc
    10201 aattgaatca gaaatggcac tattaatacc agtgatgata tcttcacttg cttttggaaa
    10261 agccatcata ggtataactg caaaaagaag tacagctact gtccctataa aggtcgtcca
    10321 gtccataact ttctcttttt tcaattgtgc tccccctaat tattaatttt atgaatcctg
    10381 tttcgattta tctcaaaatg taataattat attgattcac aaaattgaca ataactaaca
    10441 tttaataata atgcaaattt catacaattt gaaacttggc aattattgaa tatttatata
    10501 atttttcccg taataaacaa aaccttaata gcgctaaaac aatagtgtta agttatgatt
    10561 taacgaattt aacaaatttt actaggacgg catttaagaa tatttatacg ttattaacga
    10621 atatttattt attgtaaaac gctaccaaaa agttagactt ccttcccact aaaataccac
    10681 ttttctcttt caactttttt aaaaaacgga tatgcaactt ttagtattgg tatcaaaatg
    10741 attgttaggt catattctat caatatattt ttctaaagaa ttgcttttat taactttcaa
    10801 ttatgtacct aacctaaaaa gaagccaagg caacgaatgt taccttgact tctaatacat
    10861 attcaactaa ctatatattc aatcatacgc gcatgcgaga gtgattgttg tacatctata
    10921 atgcgttgat ttaaagaacc tttatatggt aaatcaggtt tgaataagtg ttgtataaat
    10981 agaccatcta ctaaaacgtc aatgtatgat aataactctc gacgttctgc acaatcattt
    11041 gctaaatatt catataaaaa tccagtccat acccaaattg tctttttatt tccaaaacgt
    11101 gctcgaaatg ctttgacaag atttaatgta atatccaaat tacaaaatgg ttcgccacct
    11161 aatagactta gcccagatat ataatcatga tcgcaatcat ctaatatttc tgctaatatt
    11221 tcatcagtgt atttctcgcc atatctgaac ttttgtgagg ctttgttata acatccaaca
    11281 caattaaatg gacatcctga tacataaaca ctgcatctta ctccttcacc gtcaacaaag
    11341 ctatttgatt ctattttagc aatataacct tgtccttgtt taatgtctaa aagtgtcatt
    11401 ctttaggcgc tttcatatgt tttactcgtg cgcaaatttc tttatgacgg cctttaatta
    11461 ctggacgttg aactggattg cctaagtaac cacatgttcg tttaacgaca tcaactgttt
    11521 taggattatc attgccacag ttcgggcatt taaatccttt ttcagttgct tcaaaatctc
    11581 catcgtaatc acattcataa caatgatcaa tcggaatatt tgtacctaag taaccaactt
    11641 tgtcataaga gtagtcccat accgcttcta gtgctttcaa attgtgttgc aatttcggat
    11701 actcacaata gtgaatgaaa ccaccactcg cataataagg ataatctttt tcaaaatcta
    11761 acttttcaaa aggtgtaaca tctttacgta catcataatg gaaagagttt tgataatatc
    11821 ctttatctgt aatgtcttta atatctccaa atctctcttg gtctaaacga caaaaacgat
    11881 ccgttagcga ttcactcggc gtactgtaaa tactgaacca aatgtcatat aattctgtcc
    11941 atttcgtttg ataacgtttc atttctttaa gaatttcaag cgtaaatgct tttgcttctt
    12001 gagatgtttc ccagtctgga ccatagaaaa cagtagctgt ttcatacaac cctatatagc
    12061 ccattgaaat cgttgcacgt ttatttttaa ataactcagc aacatcatct gtttctttta
    12121 atttatagtt aaatgcgcca cttttatata aaatcggtgc gttattcggt acagcatctt
    12181 tcaaacgatt tatacgataa agtaatgcat catgtaacac atcgatacgt tcataaaaga
    12241 tttcccagaa tttcgtcata ttaccggcag attctaatgc cattctaggt aaattaagtg
    12301 taacaacacc aagattacaa cgaccattat tttcaaaatg accttccgca tctttccaac
    12361 ttggtaaaaa tgaacgacaa cccattggcg ctttgaaatc acctaatatt tctacgagtt
    12421 tgtcataatt taaaatatct ggatacatac gtttcgttga acactttaat gctagttgtt
    12481 taatgtcata gttcggatct tgcggactaa agttggttcc ttttttaatt gaaaatacaa
    12541 gtttcgggaa aatcgctgtc gtgcggtctt ttcctaagcc tttgatacga gtatttaaga
    12601 tagcttgttg aattttgcga cttaaatgat ctgtacctaa gccgaatcct aatgttacaa
    12661 aaggtgtctg tccattagat gtatataagg tattaatttc atattctaga ctttcaatcg
    12721 catcattgat gtctttagtg acttgttgat caacataacg atcaatttca gattctttga
    12781 caaattgctt tgcgatattt ctatgttgtt cttcattatg tcgtgcatat gtactaagta
    12841 attcgtcaac gcggtcaacc gtacagccac catattgact gctggaaaca ttggctataa
    12901 tttgtacaag ctgcgctgat gcagtttgta ttgattttgg tgaagttaca ttcgcgttgc
    12961 ctatttcaaa tccattatgt agcatatttt tagcatctat taaacaacag tttgttaacg
    13021 gttggaatgg atgataatct aagtcgtgaa aatgtatgtc ccctctttga tgtgcatctg
    13081 caacgtgctt tggtaataaa taatttaatg cgtaagattt agaaacgata cctgctgtta
    13141 aatctctcat tgttgaaaat gtatcactat ctttgttggc attttcgtta acaatagcag
    13201 gatctttaga aatcaaaccc gttaatgctt cttcgatttg attcatttta ccccttcttc
    13261 ctatatattg tgtctactca ttacgttata ccactatata tagtattttc aaatttttat
    13321 tagtctgtta actaaaacgt cacaaagtta ttaacattta gtgaaattca tatttctaca
    13381 caattatttc attaattttt aaaattgttc tcgaatcgtt ctgtcatttc gacatgttta
    13441 tgcaactatt atttcatttt gcaccgggac tatagtttct atttttcata cttaaatcgt
    13501 cacaatgccc atcaacattg caattactaa cataacgatg gcgaatcccc aaatccaaaa
    13561 gaatgcatac ttaatatacg tgcccatgtt tgcctctgct aaaccaattg ccaaccataa
    13621 ggctggtgaa aacggactga caaatgtacc tataatattc cctataacca ttgaataagc
    13681 tgttgataca gacggtacac caaattgccc tgctgtttgt tcgacgatcg gtaacacagc
    13741 aaaataataa gcgtctgtgc tagttagtaa atctaatggt acgccaagta aacctacaat
    13801 aatatgcaag tatggtccta cttctgcagg aatcactttg attaaattcg tcgcaatcgc
    13861 tttaagcata ccggtttcat ttagtacacc taaaaacata cctgctgcaa taatcactgc
    13921 agccatcatt aatgcattcg gcgcatgcgc tcttaatcgc tccatttgtt catccactga
    13981 tttaaaatta ataacgagcg ccaatgagac acctatcatg aatgcaaatt caggtggcgc
    14041 aatatttatt aacatcgata gaataacagc taaagttaaa gctgtattca cccattttat
    14101 ccatgatttt gttcttgcac gtccttttac aggaaacctt acatcttgat ctcgttcata
    14161 tacttcaact aatttatgta catctatatc ttgtgtttgc ggtaattcat ttctctctat
    14221 tgcttttttg atacgtttct gttctttaaa tccaagatat accgcaaaca acataataag
    14281 aatgaatcct attatttgaa taggaattaa tccataccat aattcattga cacttttggc
    14341 ttttaacact gcagctacac gtgccattgg acctccccaa ggtaccatgt tcataatcgc
    14401 cgcgcttaat gctaatagta aaatcaataa atatttattc atatttaacg ctttatataa
    14461 aggtaataat gcaggaatag aaagcaaaaa tgttaccgca ccggccccat ctaattgggc
    14521 tattgtgcca attaaagctg tcattgcaca gacaatgacg acattgcctc gtgtcattat
    14581 tattaagcgt ttgacaagcg gcttgaataa accactatcg ttcatgatgc caaagaaaat
    14641 aatagcaaag ataaacataa taacaacgtt gatgacttga tctaaccctt tagcaaaaaa
    14701 tccaaccaaa tctgtcacac tatatcctaa aatcattgca cctaagcaag gtatgattgt
    14761 cataccaaca actggattta tctttttggc aatgagtaaa cctacaattg aaataataat
    14821 aatgagcccc attactgtta accacatatt atcactattc atattatttt ctccctccac
    14881 ttttaataca atttacataa tccccctctt taacttaaat attataatta atataatttt
    14941 ttatatttaa gttatctcat tttatcaaaa aaatcttttt tgcgtaatag ttaattcggt
    15001 tatctaattt ttatgcagta tctctaatga ctatttgaat ccaattgatt aatttgaatc
    15061 ttttcataag tatcattttt aatagcaatg gttgaattct cggttctatt acacaaaaaa
    15121 actggctaaa attagctatt attcatagca acttcaacca gtttttctaa taacctatat
    15181 atttattaag atttttcaaa tcgtctattt ctctatgtta gaaatcatct tacatatgtt
    15241 tatcatcttc gcttagaaat tattagtatg catcccatct tcatttacaa acagacatgt
    15301 tattacttca agactgcctt tagacggcac acagttatta ttttttatcc aaccacgcta
    15361 aaaattgagc ttgtttagta tcgtttataa attctttcgt cactatttgt gataacaatt
    15421 gctgcttttc gctgtgattc atatcaagta actttatttt ctgtcggcaa gtatataaaa
    15481 agtcgatata cgaactgtat gatggtggat ataacgcctc aagctctgcc ataattgatt
    15541 ttgtcaactt aggactcgca ccatcagttg atacactgat agttagcttg tcgcggtgta
    15601 gtgcacttgg aaatacaaca ttgccatttg atgcatctcc aacattatta aaaagggcat
    15661 gctcaggtaa ggcttgtttt accgcttcat tgacacgtgg ctcattggtt gctgcaataa
    15721 ctagatacgc gtctacaata tcgcttggtt caaactcttt ttctttccat atgacgacac
    15781 ctttatctac catattttga agtttttcag tgatttcagg actgataacc attatgtgtt
    15841 caacgtattg acttaatgtt tgtgcccgac gacttgcaac gaggcctcca ccaactatga
    15901 cgacattttt atttgttaaa tctatcatta atggcatatt catgttgagc aacatccttt
    15961 atatcttttt acgtcatttc atcttaaaac atttaacacc aatcgctaat aaacatcacg
    16021 ttgatagcgt tgttgctgtt tcatttgacg caatatcgtc tccgcttctt cttgagaaat
    16081 atgacgttct tttaccaata catctttaat ggcttgatgg acatctttcg ccatacattt
    16141 ttcatcacca caaatataga ttgctgcgcc ttgctcaatc cattcattga aacgttcact
    16201 ttcttccatt atacgatgct gtacatattc tttgtgttct tggtctcttg aaaatgctaa
    16261 atctacgcgt gttaagtttc cattttcaag ccattcttct atttcttctt catataaaaa
    16321 gtcagaacta cggtgttgat cgccaaagaa caaccatgtt ttcccagtca taccaagttc
    16381 ttcacgttct tgtaaatatg ctctaaaagg agcaattcca gtacctggtc caatcataat
    16441 aaccggtata tcttgcttca tcggaaattt gaagttcgga tttttcttca aataaattgg
    16501 tactatatcg cctggtttaa ttcgctcagc aaaatgaacc gagcatacac cttttctctc
    16561 acgtccatgt gcttgataac gaaccgtacc aacggtaata tgcacttcat ctggcgttgc
    16621 cataaaacta ctagaaatcg aatactctct tggtggtaat tttctaagga tttgatacat
    16681 attctcaggt tgtaattcta tagttggaaa atctgttatt aaatctataa agtcccgatt
    16741 tataacatat tcacgcgccc atgactcatc ttgaatacgt tcagataatt cttcattgtc
    16801 aaaatagata tctgcatttt tcaataatgg caatgttaat ttagtaaatt caaaatgtga
    16861 tgttagtgct tcaacaatag gaactgtatc accatgatca ttaattggca ccggagattg
    16921 cggatcccaa cctaacatgg atattagttt ttcaaccaat tcagggtcgt tttgcggtaa
    16981 tgctactata caatctcctg gttcatatga ttcactaaaa tcatcaagta aaaattctat
    17041 atgtcgtgtt tctttatttg aatcggtacc atttaaattg atattcgcta aaacttctgc
    17101 ttggtatgga tttaatttag aatatttctt ttctttggca gacttaattg attcacttat
    17161 caccgattca ctttgaatac cttctgatgt agtatcaatc atattaatga tgtctgccat
    17221 ccacttttct gcgtcttctt cataatcaat atcacaatct acacgcttac atatacgctc
    17281 agcgcctaga ttttctagta aagcatctac atctttacca gcttgacaga aaaattcata
    17341 tgtttgatca cctaaagcta gtactgaata tctcacatga tttaaattag gtgcgttatc
    17401 gtcttctaaa aattcaaaga aatcccatgc attatcaggc ggttctcctt caccatgagt
    17461 agacgtaata ataaataaat cttctaactg cgcgatgttt gtcgtatcat attcatccat
    17521 tgacatcaaa acaacttgat gtccgatatc acttaaacgt tctgaaaaaa tttcagctaa
    17581 acgcatggca ttacctgatt cagaaccata tagcactgta acatgccgct gattagcttc
    17641 gatatgtggc tctttatttt gtaacatata cgttgttgaa gttgcttgtt cttctgatat
    17701 ttgtgattgt tgccacactt ctgtctctaa ttgttgttga ttactatcac ttgtcgtatc
    17761 gttactatta gctagtaaaa aaccactaag ccactgttgt tggctctctg ttaatgtttg
    17821 cagcagattg ttaatctctg tgacttgttt ttctgtaaat ggactattag atgtatttaa
    17881 tttcaaatgt tttcacctca ataaacttga aatagatgat tgactctttt ttatcgatag
    17941 tttagtcaat tttttaatat gtatggtata aattaaaaca aacttgagtt gtgtctgata
    18001 gttatacttg caaatattta caactttcat gttgcttgaa tgacattatc agtaattcag
    18061 acaaaacttt gattatgtcg tgtcagttat taatgcatac agtaatgcat cgttgttatt
    18121 ttttatttat ttactattta ttatgtacca aatcagcctt tcagtaaaat aggtcaaatt
    18181 actgattttc taaatataaa atgcctccta atgacatact attagtacat cattaagagg
    18241 ctcttgtgtt atttgcatac taagcgctca attttaaatt taagatgaag attcttgcaa
    18301 taatatttct atatttgtcg atatatccaa tggatcttcc attggcaaga atcgatttac
    18361 aacattgcct tgtcgatcga ttataaattt tgtgaaattc cattttattg gggacccaaa
    18421 gattcctggt tgttcattct ttaaatgcgt aaataacgga tgttcatcgt tcccgttcac
    18481 agatatttta gctagcactg gaaatgtaac accaaatttc tcacgactaa ttttcaagat
    18541 ttcttcatta gaacctggtt gtcgattgtc aaaattatta ttcggaaaac tcaacactac
    18601 aaacccacga tccttatatt tttgaaacaa agtctctagt tttttcaatt gttcgctata
    18661 tatacattct gttgcagtat taacaactaa aatcacttta cctctaaatg cttctaattt
    18721 ataagttaag cctttataat cacttacttc gatatcatac acatttctat tattcataag
    18781 acacccctac acagcctttt ttatattgaa tatgttcttt ttagaatgtt ctgataaaat
    18841 aagtgcgcgt ttacaccgcg aacatacatt atatagcgtg atacattttt cgagcacacg
    18901 ataaataatg ttcgagttta tgttgttgct caacctatcc gatttaccgt cttttttcac
    18961 ctgtcttact ttattaaaat aatcacattg tatagctgta ttagtccagc tacaatatct
    19021 ttcgttataa cccctcatta taatcatcct tattttctat ttttaaaaag acaattagac
    19081 cgctctttaa actatagatt aatacttaag ttaaactcat acatactgat accatacgtt
    19141 agatctaaca atttaaaatt cgttataact atggattaaa gagctgccca actcatataa
    19201 tccttaaaaa cttcacatgt gattgtttat taagccctcc tttatcttat taaatatcct
    19261 tataaccctt ttaaaattaa actgacacac tcatacattg ttacacgcga cgataaacgt
    19321 catattattg catatcatta aaacaagtca ctttgtatcg tgtgcgaccg aatacatatt
    19381 aaatcatcaa taatgattat tcaattatta aaaataaaaa cgaacattct atatagagtt
    19441 tctacttatt gttttttcat cattgtctac attacttaac attcgtgttt ataagtaata
    19501 ttgaccgcca atatttagac actttataag tatgccattc atcattttta atcatattat
    19561 ttttcttatt gatatttcta ttcatatatt tctgattgta tatttagata taataatttg
    19621 ttattggaaa atatatgaaa caaatttgac tcatcttttt caatatctaa atgacacgat
    19681 gcatgatttt tgaattttgt aattaacttc cttttaaatt agactgaatt atgacaattt
    19741 tcaaacattt taatgtgtaa atcattggca aaatatacga attgcattta aatcgtcaat
    19801 ttacatatat ttttgcgtag taatcaatcg ttatcattgt tatttatcgt tacattattt
    19861 cgagtatcag tatgtatttc gggcttcgtt tgataacgac atttctttgt gacatcgctt
    19921 catcagtgta acaacaaata caatgatttc gtgatgttag ttacccattt tatgtgttgc
    19981 ataaaatatg ttgttataaa acattttaaa tcattttata taaacaatct atatattttt
    20041 ggcatttcca aaatatcact tgttatatta aaaaccgaca agacatttta tcttatcggt
    20101 tgaaatttgt tattgttatt tgtaatgttt ttaggtttct ttttaatata atatatttca
    20161 gtgaaaatac atgattgatt gtgatttcac tgaaacatgg ttaattgcgt tgttgatgaa
    20221 taactttagc ataaatatag gaagttattt tgtacatcgc catatataga aacgaaatta
    20281 taatgacagc tagtacgtaa cttgttaaaa atatatgatg gttattaata cctatcatat
    20341 ttagtaacgt atatacaatg ttactagaaa ttaatgtgtg aatcagtgct actgttattg
    20401 gtattgcgaa caagaaagtc atttgatttc gtgttatctt tgctattctt ccattatcta
    20461 aaccaagttt tttcataatg tcataattgt gctgatttgc ataggcttct gaaacttgtt
    20521 tatagtacat cattagaaat attccaatga caagtacaat tgatactacg ccacctacaa
    20581 atattagtcc acctgtcaaa cgatcccaaa ccattagcat ttctgattta gaatttattg
    20641 acgcatcgta ttttttcgat agcttttgta tttgtgatgt tgttaatttc gtctttccaa
    20701 atacattaaa gtttatcgat attgtcgaat tcttttgttc atgtgcataa aatcctttta
    20761 ctttttgtaa ttgattgtta tctttaacaa ttaaaaccat actatcttga atcatcatca
    20821 aatttaacgc atgtgcgtca agttgtttca ctttaaagac ttcattatta agaccgacct
    20881 tagactgctt tttaaaaata ggtacattcg taatcatacc tatactttga ttgtctaagt
    20941 gtaatttgtt ttggtgatat cgattataat cttgtgcagt aaaagctgta atcattactg
    21001 attgtcttga aaatatcgcg ccttcattac caatgaagtc tattggaata tcatcactag
    21061 cctgttttaa cttgtaataa gcacgattct tttctaaaga agctctaaat aatgtcgttt
    21121 catatacttt gaagtctttt gcattaaccg aaccctgtat atcacgttta atattctcaa
    21181 ttgtttgttg acgttctatt tgtgacttag aattgtcgct aaatgacaaa tcataatcat
    21241 tcgtaatcaa tttcgtaatg gtatgattca tatcacgata ggttgttaat gtcattgtga
    21301 gcgttacaat caaaaatgta cacagcaaag tgattgttgc aagactgact gcattcattt
    21361 taagacgtac acgcatccca actaccacaa aaaaataccg tggatgatag taaacttttg
    21421 atacctgctg caatatacta ataattattt cacttatacc tacaaaaaat gcataagtac
    21481 cgataataac taatcctatg acaatccata ttttaaaaaa ggcaccaaac gtcgtatctt
    21541 gttgtaatgc aataaagtag cctaaatata gtgctgcgct tcctataaca attaaaacgt
    21601 aacgcaacca tcgtgatatg acactagaat cggaacgatg ttgataagtt atcggccgtt
    21661 gaaaattaat actgaacaag ttgaatataa gtagcatgcc cattaataca gcaatgataa
    21721 tcaaagtaat aaacatcgct acaaagtcaa atggataata ccttaacgtc gcaacttcac
    21781 tgcccattat tttctgtata aacaagaaaa atatcgcacc aagtaagtat ccgccggcaa
    21841 tactaacgac tgaaattatt ataaattgta agatagtttc cattactaca attaaacgca
    21901 tacttttctt ggtcatgccc aatgtcataa aaatgctaaa ctcttttcgt ctttgtgaca
    21961 tcataaagtg atttgcatag aaaataaaaa taaaagttaa aagcgccata aaaaaattag
    22021 ctatgataat aaatggtact aggaagtcat tcttctgttt tatgtagcta tttaacccaa
    22081 ttgacacaag cgtatattca atcatgaata gtacacttac cgctaaaatg aatggaatga
    22141 ttacaagtcg ctgcgtaata aattgacgtc gcactatgtg ccataacaat cttaacttca
    22201 tatattgaca cttcctccat tcacaagtgc taagctatct gttattcgtt gttgaaaagc
    22261 taattgactt tcttcgccac gatatatttc atgatataga cgcccatctt taataaaaat
    22321 tactcgctct gcataagacg catcgatatt tgaatgagtt accattaaaa ttgtctgttc
    22381 caattgatga atctcttgaa ataacatcat caatgccttt gatgtttttg aatcaagtgc
    22441 acctgtaggt tcatcggcta gtaaaatcgt cggtttagta actaatgcac gtgcaatggc
    22501 aatgcgttgc ttctgacccc ccgagatttc agaaggatac ttgtttaaga atccctctaa
    22561 atgtaattgt actgccaact gatgtaccct ttgttctata tcttttcgtt tagcaccagc
    22621 taaaattaat ggcatcatta tattttcttt attcgtcatt gttggtaaaa gattaaaatc
    22681 ttgaaaaaca aaacctacca tttgttgacg atacagtgct ttacttttat ttttcatatt
    22741 atttaaatgt gcgccatcca caataatgtc accttcagtt agtccatcaa aagaagcaat
    22801 taaatttagt agtgtagact tccctgaccc agactcaccc ataattgcaa caaattcacc
    22861 agctccaact gataaattca tttgattaag tgctgtcgta gcattcaaac ctttaccata
    22921 aacctttttt acatgtttca cttctagcaa catggcatgc acctcatttc tttgttagtt
    22981 ctatattaaa acaaaaaaat actaataaca tttaaattag tgaaaaaatg ttactagtat
    23041 gcttacaata ttgaaagttt ttattcatct ggaaattgaa tcgtaaatgt tgtaccctca
    23101 ttttgtttag atacgactga aacaggatgg tttgtgtgag ttgaaatttg ttttacgata
    23161 aataagccaa tcccacttga gttactttga cgctggccat tataacctga atagccctta
    23221 tcaaatattt taggcaagtc cgcttcacta ataccgatac cattatcttt tacgtataat
    23281 tgattggatt gctcatcaaa ttcaatccat atatctttac ctctcgcata cttaagtgca
    23341 ttatttatta attgttctat cagtaaagag gtccatctaa cgtcagttaa tacttcgtga
    23401 tgacaaggtt cataatggat ttttgttttt tgatcaataa actgtattga atatttcata
    23461 ataattgggc gaatgatatt gttaatcgaa attttagtga cagaaatatc agaagtttca
    23521 tttaataact ttaaataact aagtgctaaa cttgtatagt tatcaatttg aataacctct
    23581 tgacgaacac gattcacaac attaggctca tctctttcaa gtaacagttg tgctgcagta
    23641 atgggtgttt tcatttgatg tacccatgtt aaaaagtagc tctctacatc atttttatat
    23701 tcaatttgtt catttttaag ctgatacaac gcattttcta agttttcaat ttgttgttgt
    23761 tggcttatag tttttacaaa acttaaatat ttaataccta tgaatatgag taataataat
    23821 aaaataacgc caagtgctag taaatatgct tctaatggca aatgatacag gtaaaacatt
    23881 aagccaaata aagcaaaaat aactatgact attgctattt cctgagtaat acttttaaga
    23941 aaggtcattg cattcaccct atactttata tccgacattt ttctttgtaa taataaaatc
    24001 attaacgcca atagtattta attttttacg caggcgcgtc atgttaacag ctaatgtgtt
    24061 atcatctatg aagttttctg attcccaaca tttttcaatt aaagcagttc tacttacata
    24121 tttatcttca ttttgaaata ataactttaa tatttgtaat tcagtcaaag atagctgtat
    24181 gttttgttct tgatacacga cttttgcttc atctaatatt aatgtacaac ctttaaccgt
    24241 caatgaatca ttagctactg acaagtcata agttcgtctc aataatgctt gaattttggc
    24301 aatcgttaat gacaagttaa atggtttttc gataaaatca tctcccccca tttgtattgc
    24361 cataatttgg tccatattat caatacggga actaataaat ataattggca cattagatgt
    24421 ttttcggatt tcttgacacc aatgaaaacc atttaacgtt ggcaaattaa tatctagcaa
    24481 tacaagctga ggttgatgtt ggttaaagat agacagtata tcatcaaatt gttcaacgac
    24541 aataacaccg taattccatt ttttaagttc agatgctaaa ctttctgcta taacaaaatc
    24601 atcttcaaca attaatattt tcatctataa tcttcttcct tcaattgaat taccctttgt
    24661 tatctttttg ttctttagcc tgttgcaatg acggctcttg atttgaactt tccgtgtcat
    24721 ttgtattttg atttagtgta tttgcttttt ctttctcgag ttgctttttc ttcgtacgtt
    24781 tacgctcctc taaaatagac aacactataa agaatatgat taataatgga gataacatta
    24841 ctagtatcat ttttccacct caaattatat ttaat
//
LOCUS       DAMZKC010000023        23859 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.023, whole genome shotgun sequence.
ACCESSION   DAMZKC010000023 DAMZKC010000000
VERSION     DAMZKC010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 23859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23859
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.023"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(37..426)
                     /locus_tag="POB55_000675"
     CDS             complement(37..426)
                     /locus_tag="POB55_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499660.1"
                     /translation="MKNGPKLSLALIGIFLILCEFFYGIPFLGATFILSFGWQPLIFN
                     ALLYLILTIILLVNRQNAIRPIAIIPIFGIVGSFLAIIPFLGILIHWILFFLMILFVL
                     VVLSAPTYIPNKNARVIYTQYKDGHRE"
     gene            783..1091
                     /gene="rpsJ"
                     /locus_tag="POB55_000676"
     CDS             783..1091
                     /gene="rpsJ"
                     /locus_tag="POB55_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HDI6499661.1"
                     /translation="MAKQKIRIRLKAYDHRVIDQSAEKIVETAKRSGADVSGPIPLPT
                     EKSVYTIIRAVHKYKDSREQFEQRTHKRLIDIVNPTPKTVDALMGLNLPSGVDIEIKL
                     "
     gene            1119..1781
                     /gene="rplC"
                     /locus_tag="POB55_000677"
     CDS             1119..1781
                     /gene="rplC"
                     /locus_tag="POB55_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HDI6499662.1"
                     /translation="MTKGILGRKIGMTQVFGENGELIPVTVVEAKENVVLQKKTVEVD
                     GYNAIQVGFEDKKAYKKDAKSNKYANKPAEGHAKKADAAPKRFIREFRNVDVDAYEVG
                     QEVSVDTFVAGDVIDVTGVSKGKGFQGAIKRHGQSRGPMSHGSHFHRAPGSVGMASDA
                     SRVFKGQKMPGRMGGNTVTVQNLEVVQVDTENKVILVKGNVPGPKKGLVEIRTSIKKG
                     NK"
     gene            1808..2431
                     /gene="rplD"
                     /locus_tag="POB55_000678"
     CDS             1808..2431
                     /gene="rplD"
                     /locus_tag="POB55_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HDI6499663.1"
                     /translation="MANYDVLKLDGTKSGSIELSDAVFGIEPNNSVLFEAINLQRASL
                     RQGTHAVKNRSAVSGGGRKPWKQKGTGRARQGTIRAPQWRGGGIVFGPTPRSYAYKMP
                     KKMRRLALRSALSFKAQENGLTVVDAFNFEAPKTKEFKNVLSTLEQPKKVLVVTENED
                     VNVELSARNIPGVQVTTAQGLNVLDITNADSLVITEAAAKKVEEVLG"
     gene            2431..2706
                     /gene="rplW"
                     /locus_tag="POB55_000679"
     CDS             2431..2706
                     /gene="rplW"
                     /locus_tag="POB55_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002506966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HDI6499664.1"
                     /translation="MEARDILKRPVITEKSSEAMAEDKYTFDVDTRVNKTQVKMAVEE
                     IFNVKVASVNIMNYKPKKKRMGRYQGYTNKRRKAIVTLKEGSIDLFN"
     gene            2739..3572
                     /gene="rplB"
                     /locus_tag="POB55_000680"
     CDS             2739..3572
                     /gene="rplB"
                     /locus_tag="POB55_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HDI6499665.1"
                     /translation="MAIKKYKPITNGRRNMTSLDFAEITKTTPEKSLLKPLPKKAGRN
                     NQGKLTVRHHGGGHKRQYRVIDFKRNKDGINAKVDSIQYDPNRSANIALVVYADGEKR
                     YIIAPKGLEVGQIVESGAEADIKVGNALPLQNIPVGTVVHNIELKPGKGGQIARSAGA
                     SAQVLGKEGKYVLIRLRSGEVRMILSTCRATIGQVGNLQHELVNVGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGRAPIGRPSPMSPWGKPTLGKKTRRGKKSSDKLIVRGRKK
                     K"
     gene            3639..3917
                     /gene="rpsS"
                     /locus_tag="POB55_000681"
     CDS             3639..3917
                     /gene="rpsS"
                     /locus_tag="POB55_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HDI6499666.1"
                     /translation="MARSIKKGPFVDEHLMKKVEAQEGSEKKQVIKTWSRRSTIFPNF
                     IGHTFAVYDGRKHVPVYVTEDMVGHKLGEFAPTRTFKGHVADDKKTRR"
     gene            3946..4299
                     /gene="rplV"
                     /locus_tag="POB55_000682"
     CDS             3946..4299
                     /gene="rplV"
                     /locus_tag="POB55_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HDI6499667.1"
                     /translation="MEAKAVARTIRIAPRKVRLVLDLIRGKNAAEAIAILKLTNKASS
                     PVIEKVLMSALANAEHNYDMNTDELVVKEAYANEGPTLKRFRPRAQGRASAINKRTSH
                     ITIVVSDGKEEAKEA"
     gene            4323..4976
                     /gene="rpsC"
                     /locus_tag="POB55_000683"
     CDS             4323..4976
                     /gene="rpsC"
                     /locus_tag="POB55_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HDI6499668.1"
                     /translation="MGQKINPIGLRVGIIRDWEAKWYAEKDFASLLHEDLKIRKFIDN
                     ELKEASVSHVEIERAANRINIAIHTGKPGMVIGKGGSEIEKLRNKLNALTDKKVHINV
                     IEIKKVDLDARLVAENIARQLENRASFRRVQKQAITRAMKLGAKGIKTQVSGRLGGAD
                     IARAEQYSEGTVPLHTLRADIDYAHAEADTTYGKLGVKVWIYRGEVLPTKNTSGGGK"
     gene            4979..5413
                     /gene="rplP"
                     /locus_tag="POB55_000684"
     CDS             4979..5413
                     /gene="rplP"
                     /locus_tag="POB55_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HDI6499669.1"
                     /translation="MLLPKRVKYRRQHRPKTTGRSKGGNYVTFGEFGLQATTTSWITS
                     RQIESARIAMTRYMKRGGKVWIKIFPHTPYTKKPLEVRMGAGKGAVEGWIAVVKPGRI
                     LFEVAGVSEEVAREALRLASHKLPVKTKFVKREELGGETNES"
     gene            5403..5612
                     /gene="rpmC"
                     /locus_tag="POB55_000685"
     CDS             5403..5612
                     /gene="rpmC"
                     /locus_tag="POB55_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002509269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HDI6499670.1"
                     /translation="MKAKEIRDLTTSEIEEQIKSSKEELFNLRFQLATGQLEETARIR
                     TVRKTIARLKTVAREREIEQSKANQ"
     gene            5636..5899
                     /gene="rpsQ"
                     /locus_tag="POB55_000686"
     CDS             5636..5899
                     /gene="rpsQ"
                     /locus_tag="POB55_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011983253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HDI6499671.1"
                     /translation="MSERNDRKVYVGKVVSDKMDKTITVLVETYKTHKLYGKRVKYSK
                     KYKTHDENNSAKLGDIVKIQETRPLSATKRFRLVEIVEESVII"
     gene            5931..6299
                     /gene="rplN"
                     /locus_tag="POB55_000687"
     CDS             5931..6299
                     /gene="rplN"
                     /locus_tag="POB55_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HDI6499672.1"
                     /translation="MIQQETRLKVADNSGAREVLTIKVLGGSGRKTANIGDVIVCTVK
                     NATPGGVVKKGDVVKAVIVRTKSGVRRNDGSYIKFDENACVIIRDDKGPRGTRIFGPV
                     ARELREGNFMKIVSLAPEVL"
     gene            6335..6652
                     /gene="rplX"
                     /locus_tag="POB55_000688"
     CDS             6335..6652
                     /gene="rplX"
                     /locus_tag="POB55_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HDI6499673.1"
                     /translation="MHIKKGDNVKVIAGKDKGKEGKVIATLPKKDRVVVEGVNIMKKH
                     QKPTQLNPEGGILETEAAIHVSNVQLLDPKTNEPTRVGYKFVDGKKVRIAKKSGEEIK
                     SNN"
     gene            6679..7218
                     /gene="rplE"
                     /locus_tag="POB55_000689"
     CDS             6679..7218
                     /gene="rplE"
                     /locus_tag="POB55_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HDI6499674.1"
                     /translation="MNRLKEKFNTEVTENLMKKFNYSSVMEVPKIDKIVVNMGVGDAV
                     QNSKVLDNAVEELELITGQKPLVTKAKKSIATFRLREGMPIGAKVTLRGERMYEFLDK
                     LISVSLPRVRDFQGVSKKAFDGRGNYTLGVKEQLIFPEIDYDKVSKVRGMDIVIVTTA
                     NTDEEARELLANFGMPFRK"
     gene            7241..7426
                     /locus_tag="POB55_000690"
     CDS             7241..7426
                     /locus_tag="POB55_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HDI6499675.1"
                     /translation="MAKTSMVAKQQKKQKYAVREYTRCERCGRPHSVYRKFKLCRICF
                     RELAYKGQIPGVRKASW"
     gene            7458..7856
                     /gene="rpsH"
                     /locus_tag="POB55_000691"
     CDS             7458..7856
                     /gene="rpsH"
                     /locus_tag="POB55_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HDI6499676.1"
                     /translation="MTMTDPIADMLTRVRNANMVRHEKLELPASNIKKEIAEILKSEG
                     FIKNVEYVEDDKQGVLRLFLKYGQNDERVITGLKRISKPGLRVYAKASEMPKVLNGLG
                     IALVSTSEGVITDKEARKRNVGGEIIAYVW"
     gene            7881..8417
                     /gene="rplF"
                     /locus_tag="POB55_000692"
     CDS             7881..8417
                     /gene="rplF"
                     /locus_tag="POB55_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HDI6499677.1"
                     /translation="MSRVGKKIIDIPSDVTVTFDGNHVTVKGPKGELSRTLNERMTFK
                     QEENTIEVVRPSDSKEDRTNHGTTRALLNNMVQGVSQGYVKVLELVGVGYRAQMQGKD
                     LILNVGYSHPVEIKAEENITFSVEKNTVVKVEGISKEQVGALASNIRSVRPPEPYKGK
                     GIRYQGEYVRRKEGKTGK"
     gene            8448..8807
                     /gene="rplR"
                     /locus_tag="POB55_000693"
     CDS             8448..8807
                     /gene="rplR"
                     /locus_tag="POB55_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HDI6499678.1"
                     /translation="MISKIDKNKVRLKRHARVRTNLSGTAEKPRLNVYRSNKHIYAQI
                     IDDNKGVTLAQASSKDSDIATTATKVELATKVGEAIAKKAADKGIKEIVFDRGGYLYH
                     GRVKALAEAARESGLEF"
     gene            8828..9328
                     /gene="rpsE"
                     /locus_tag="POB55_000694"
     CDS             8828..9328
                     /gene="rpsE"
                     /locus_tag="POB55_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016426364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HDI6499679.1"
                     /translation="MARREEETKEFEERVVTINRVAKVVKGGRRFRFTALVVVGDKNG
                     RVGFGTGKAQEVPEAIKKAVEAAKKDLVVVPRVEGTTPHTITGRYGSGSVFMKPAAPG
                     TGVIAGGPVRAVLELAGITDILSKSLGSNTPINMVRATIDGLQNLKNAEDVAKLRGKT
                     VEELYN"
     gene            9345..9524
                     /gene="rpmD"
                     /locus_tag="POB55_000695"
     CDS             9345..9524
                     /gene="rpmD"
                     /locus_tag="POB55_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HDI6499680.1"
                     /translation="MAKLQITLTRSVIGRPETQRKTVEALGLKKTNSSVVVEDNPAIR
                     GQINKVKHLVTVEEK"
     gene            9541..9981
                     /gene="rplO"
                     /locus_tag="POB55_000696"
     CDS             9541..9981
                     /gene="rplO"
                     /locus_tag="POB55_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HDI6499681.1"
                     /translation="MKLHELKPAEGSRKERNRVGRGVATGNGKTSGRGHKGQKARSGG
                     GVRPGFEGGQLPLFRRLPKRGFTNINRKEYAIVNLDQLNKFEDGTEVTPALLVESGVV
                     KNEKSGIKILGNGSLDKKLTVKAHKFSASAAEAIDAKGGAHEVI"
     gene            9981..11273
                     /gene="secY"
                     /locus_tag="POB55_000697"
     CDS             9981..11273
                     /gene="secY"
                     /locus_tag="POB55_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HDI6499682.1"
                     /translation="MIQTLVNFFRTKEVRNKIFFTLAMLVIFKIGTYIPAPGVNPAAF
                     DNPQGSQGATELLNTFGGGALKRFSIFAMGIVPYITASIVMQLLQMDIVPKFSEWAKQ
                     GEVGRRKLNNVTRYLAISLAFIQSIGMAFQFNNYLKGALIINQSIMSYLLIALVLTAG
                     TAFLIWLGDQITQFGVGNGISIIIFAGILSTLPASLIQFGQTAFVGQEDTSLAWLKVL
                     GLLVSLILLTVGAIYVLEAVRKIPIQYAKKQTAQRLGSQATYLPLKVNSAGVIPVIFA
                     MAFFLLPRTLTLFYPDKEWAQNIANAANPSSNVGMVVYIVLIILFTYFYAFVQVNPEK
                     MADNLKKQGSYVPGIRPGEQTKKYITKVLYRLTFVGSIFLAVISILPILATKFMGLPQ
                     SIQIGGTSLLIVIGVAIETMKSLEAQVSQKEYKGFGGR"
     gene            11290..11937
                     /locus_tag="POB55_000698"
     CDS             11290..11937
                     /locus_tag="POB55_000698"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HDI6499683.1"
                     /translation="MNIILMGLPGAGKGTQASEIVKKFPIPHISTGDMFRKAIKEETE
                     LGKEAKSYMDRGELVPDEVTVGIVKERISEDDAKKGFLLDGFPRTIEQAEALNNIMSE
                     LDRNIDAVINIEVPEEELMNRLTGRRICESCGTTYHLVFNPPKVEGICDIDGGKLYQR
                     EDDNPETVANRLSVNIKQSKPILDFYDQKGVLKNIDGSKDISDVTKDVIDILDHL"
     gene            12130..12348
                     /gene="infA"
                     /locus_tag="POB55_000699"
     CDS             12130..12348
                     /gene="infA"
                     /locus_tag="POB55_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HDI6499684.1"
                     /translation="MAKQDVIELEGTVLDTLPNAMFKVELENGHEILAHVSGKIRMNY
                     IRILPGDKVTVEMSPYDLTRGRITYRYK"
     gene            12380..12493
                     /gene="rpmJ"
                     /locus_tag="POB55_000700"
     CDS             12380..12493
                     /gene="rpmJ"
                     /locus_tag="POB55_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HDI6499685.1"
                     /translation="MKVRPSVKPICEKCKVIKRKGKVMVICENPKHKQRQG"
     gene            12516..12881
                     /gene="rpsM"
                     /locus_tag="POB55_000701"
     CDS             12516..12881
                     /gene="rpsM"
                     /locus_tag="POB55_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HDI6499686.1"
                     /translation="MARIAGVDIPREKRVVISLTYIYGIGTSTAQKILEEANVSADTR
                     VKDLTDDELGRIREVVDGYKVEGDLRRETNLNIKRLMEISSYRGIRHRRGLPVRGQKT
                     KNNARTRKGPVKTVANKKK"
     gene            12905..13294
                     /gene="rpsK"
                     /locus_tag="POB55_000702"
     CDS             12905..13294
                     /gene="rpsK"
                     /locus_tag="POB55_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016998822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HDI6499687.1"
                     /translation="MARKQVSRKRRVKKNIENGVAHIRSTFNNTIVTITDEFGNALSW
                     SSAGALGFKGSKKSTPFAAQMASETASKSAMEHGLKTVEVTVKGPGPGRESAIRALQS
                     AGLEVTAIRDVTPVPHNGCRPPKRRRV"
     gene            13369..14313
                     /locus_tag="POB55_000703"
     CDS             13369..14313
                     /locus_tag="POB55_000703"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HDI6499688.1"
                     /translation="MIEIEKPRIETIEISEDAKFGKFVVEPLERGYGTTLGNSLRRIL
                     LSSLPGAAVKYIEIEGVLHEFSAVDNVVEDVSTIIMNIKQLALKIYSEEDKTLEIDVR
                     DEGEVTASDITHDSDVEILNPELKIATVSKGGHLKIRLVANKGRGYALAEQNNTSDLP
                     IGVIPVDSLYSPVERVNYTVENTRVGQSSDFDKLTLDVWTNGSITPQESVSLAAKIMT
                     EHLNIFVGLTDEAQNAEIMIEKEEDQKEKVLEMSIEELDLSVRSYNCLKRAGINSVQE
                     LADKSEADMMKVRNLGRKSLEEVKYKLEDLGLGLRKED"
     gene            14330..14698
                     /gene="rplQ"
                     /locus_tag="POB55_000704"
     CDS             14330..14698
                     /gene="rplQ"
                     /locus_tag="POB55_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002450507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HDI6499689.1"
                     /translation="MGYRKLGRTSDQRKAMLRDLATSLIISERIETTEARAKEVRSVV
                     EKLITLGKKGDLASRRNAAKTLRNVEILNEDETTQTALQKLFGEIAERYTERQGGYTR
                     ILKQGPRRGDGAESVIIELV"
     gene            15236..16045
                     /locus_tag="POB55_000705"
     CDS             15236..16045
                     /locus_tag="POB55_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HDI6499690.1"
                     /translation="MEDKNSVIVFKNVSFQYQSDASFTLKDVSFSIPKGQWTSIVGHN
                     GSGKSTIAKLMIGIEKVKSGEIFYNNQTITDDNFEKLRKDIGIVFQNPDNQFVGSIVK
                     YDVAFGLENHAVPHDEMHRRVGEALKQVDMLERADYEPNALSGGQKQRVAIASVLALN
                     PSVIILDEATSMLDPDARQNLLDLVRKVKSEHNITIISITHDLSEAMEADHVIVMNKG
                     TVYKEGTAIEIFDHAEELTTIGLDLPFPIKINQMLGYQTSFLTYEGLVDQL"
     gene            16042..16902
                     /locus_tag="POB55_000706"
     CDS             16042..16902
                     /locus_tag="POB55_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HDI6499691.1"
                     /translation="MTIRFDNVSYTYQKGTPYQHQAIHDVNTEFEQGKYYAIVGQTGS
                     GKSTLIQNINALLKPTTGTVTVDDITITHKTKDKYIRPVRKRIGMVFQFPESQLFEDT
                     VEREMIFGPKNFKMNLDEAKNYAHRLLMDLGFSRDVMSQSPFQMSGGQMRKIAIVSIL
                     AMNPDIIVVDEPTAGLDPQSKRQVMRLLKSLQTDENKAIILISHDMNEVARYADEVIV
                     MKEGSIVSQTSPKELFKDKKKLADWHIGLPEIVQLQYDFEQKYQTKLKDIALTEEAFV
                     SLYKEWQHEK"
     gene            16892..17698
                     /locus_tag="POB55_000707"
     CDS             16892..17698
                     /locus_tag="POB55_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDI6499692.1"
                     /translation="MKNKLIIGRYLPINSFVHHLDPRAKLMFVFLFIILIFFCHSPLT
                     YLWVFALILFIMRLAKIQLWFLIKGLTPIFFFLIFTLMMHIFLTKGGYVLVEWHGITI
                     ETNGILEGLYISLRLIGIVMIATIMTLSTSPIDLTDAFERLLAPLKMFKLPVHQLSMI
                     MSIALRFIPTLMDELDKIILAQKSRGSEISSGNIATRIKSFIPLLVPLFISAFQRAEE
                     LAVAMEVRGYDANVKRTSYRQLKWQLRDTLSLIMIIPIAIILFVLKYSGV"
     gene            17703..18506
                     /gene="truA"
                     /locus_tag="POB55_000708"
     CDS             17703..18506
                     /gene="truA"
                     /locus_tag="POB55_000708"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDI6499693.1"
                     /translation="MRILVEIAYQGNNFLGFQIQQNGRTVQQQFEKLLQRMHKRHVRI
                     HPSSRTDRGVHAIQQYFHFDTELNIPMSQWQYAMNRTLPDDIYVNNVVTVDDDFHCRY
                     DCVGKRYRYKVYQAQHRDPFQSGLKTFIPETLDLDKMNRAAQQFIGTHDFTGFCSQKT
                     EVESKVRTLYQSEIVKTDDGFDYIVTGSGFLYNMVRVLVAFLIEVGKGRHEISDVPKL
                     LESKNRKNVPFTATAEGLYLEKIYLDENELLKDFGNDIKIHRKKSLQND"
     gene            18746..19183
                     /gene="rplM"
                     /locus_tag="POB55_000709"
     CDS             18746..19183
                     /gene="rplM"
                     /locus_tag="POB55_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019468667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HDI6499694.1"
                     /translation="MRQTFMANESNIERKWYVIDAEGQTLGRLSSEVASILRGKNKVT
                     YTPHVDTGDYVIVINASKIEFTGNKETDKVYYRHSNHPGGIKSITAGELRRTNPERLI
                     ENSIKGMLPSTRLGEKQGKKLFVYGGAEHPHAAQQPENYELRG"
     gene            19197..19595
                     /gene="rpsI"
                     /locus_tag="POB55_000710"
     CDS             19197..19595
                     /gene="rpsI"
                     /locus_tag="POB55_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDI6499695.1"
                     /translation="MTLAQVEYRGTGRRKNSVARVRLVPGEGNITVNNRDVREYLPFE
                     SLILDLNQPFDVTETKGNYDVLVNVHGGGFTGQAQAIRHGIARALLEADPEYRGSLKR
                     AGLLTRDPRMKERKKPGLKAARRSPQFSKR"
     gene            19942..20124
                     /locus_tag="POB55_000711"
     CDS             19942..20124
                     /locus_tag="POB55_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499696.1"
                     /translation="MKDFIFWKKAFIPVYFIVAMLVFLLFRFYIKTDNFSMYLMIIFL
                     ICLGTASIIYNYKNNR"
     gene            20571..22235
                     /gene="alsS"
                     /locus_tag="POB55_000712"
     CDS             20571..22235
                     /gene="alsS"
                     /locus_tag="POB55_000712"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HDI6499697.1"
                     /translation="MTDKKYTAADMVIDTLKNNGVEYVFGIPGAKIDYLFNALIDDGP
                     ELIVTRHEQNAAMMAQGIGRLTGKPGVVLVTSGPGVSNLTTGLLTATSEGDPVLALGG
                     QVKRNDLLRLTHQSIDNAALLKYSSKYSEEVQDPESLSEVMTNAIRIATSGKNGASFI
                     SIPQDVISSPVESKAISLCQKPNLGVPSEQDINDVIEAIKNASFPVLLAGMRSSSADE
                     TNAIRKLVERTNLPVVETFQGAGVISRELENHFFGRVGLFRNQVGDELLRKSDLVVTI
                     GYDPIEYEASNWNKELDTQIINIDEVQAEITNYMQPKKELIGNIAKTIEMISDKVDEP
                     FINQQHLDELEQLRAHIDEETGIKATHEEGILHPVEIIESMQKVLTDETTVTVDVGSH
                     YIWMARNFRSYNPRHLLFSNGMQTLGVALPWAISAALVRPNTQVVSVAGDGGFLFSSQ
                     DLETAVRKNLNIIQLIWNDGKYNMVEFQEEMKYKRSSGVDFGPVDFVKYAESFGAKGL
                     RVTNQEELEAAIKEGYETDGPVLIDIPVNYKDNIKLSTNMLPDVFN"
     gene            22272..22976
                     /gene="budA"
                     /locus_tag="POB55_000713"
     CDS             22272..22976
                     /gene="budA"
                     /locus_tag="POB55_000713"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HDI6499698.1"
                     /translation="MASVLYQHGTLGTLMAGLLKGTASINELLQHGDLGIATLTGSNG
                     EVIFLDGKAYHANEHKEFVELKGDELTPYATVTKFVADTSYETKDKSSEAVFAEIKEK
                     MLSENLFSAVKISGLFKKMHVRMMPAQEPPYTRLIDSARRQPEQTETYVKGSVVGFFT
                     PELFHGIGSAGFHVHFANDDRNFGGHVLDFEVEDVKVEIQNIETFEQHFPIQDEDFIN
                     ANIDYKDIADEIREAE"
     gene            complement(23363..23788)
                     /locus_tag="POB55_000714"
     CDS             complement(23363..23788)
                     /locus_tag="POB55_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAP domain-containing protein"
                     /protein_id="HDI6499699.1"
                     /translation="MKLKSFVTATLALGLLSTVGAALPSHEASADSNNGYKELTMDGK
                     HTVPYTISVDGITALHRTYFVFPENKKVLYQEIDSKVKNELASQRGVTTEKINNAQTA
                     TYTLTLNDGNKKVVNLKKNDDAKNSIDPSTIKQIQIVVK"
BASE COUNT         8571 a         3759 c         4619 g         6910 t
ORIGIN      
        1 tctatattat atacctgatt aaaaaggttt taagaattat tctctatgtc catctttgta
       61 ttgtgtgtaa atgacacgag catttttatt aggtatatag gttggcgctg ataatacaac
      121 taggacaaat agaatcatta ggaaaaataa aatccaatga atcaaaattc ctaagaaggg
      181 gatgatagct aaaaaactac ctactatacc aaatattgga ataatcgcta taggtctaat
      241 cgcattttgt ctattcacta ataatataat agtaagaatt agataaagta atgcattaaa
      301 gattaaaggc tgccatccaa aacttaaaat aaacgttgca cctaaaaaag gaatgccata
      361 gaaaaactca caaagtatta aaaagatacc tataagtgct aaagataatt ttggtccatt
      421 tttcatattc attcaccacc gttcttatga ctaattatat agaataattt tcaatgataa
      481 taataaaatg attcaaggta cgaatttaca ataaagaagg aaataaaaaa tatctcgaaa
      541 atagttgaac tgactaagta tataaagtaa aatataaaag tatgtgttag acagatagaa
      601 aaattatgtg aaaagccttg atttatcgat gtttttcgtg taatataact aacgttggac
      661 tttaagaaaa gcgatgaagc gaaaggttac tgacacaccc ggccgctttg ccatggcgct
      721 gtgtaagata gttttcgtgg agaagtctat cactaaatgt agacgaataa ggagggaaaa
      781 ttatggcaaa acaaaaaatc agaatcagat taaaagctta tgatcaccgc gtgattgatc
      841 aatcagcaga gaagattgta gaaacagcga aacgttctgg tgcagatgtt tctggaccaa
      901 ttccgttacc aactgagaaa tcagtttaca caatcatccg tgccgtgcat aagtataaag
      961 attcacgtga acaattcgaa caacgtacac acaaacgttt aatcgatatt gtaaacccaa
     1021 caccaaaaac agttgacgct ttaatgggct taaacttacc atctggtgta gacatcgaaa
     1081 tcaaattata atagacaatt ttaggaggtg gactttcgat gaccaaagga atcttaggaa
     1141 gaaaaattgg gatgacacaa gtattcggag aaaacggtga attaatccct gtaacagtag
     1201 tagaagctaa agaaaatgtt gtattacaaa agaaaactgt agaagttgat ggatacaacg
     1261 caatccaagt tggatttgaa gacaaaaaag catacaaaaa agatgcaaaa tctaataaat
     1321 atgctaataa accagctgaa ggtcacgcta aaaaagctga cgcagcacct aagcgcttca
     1381 ttcgtgaatt ccgcaatgta gacgtggatg cttacgaagt aggtcaagaa gtctcagtag
     1441 atacttttgt agctggcgac gttattgacg taacaggcgt atcaaaaggt aaaggtttcc
     1501 aaggtgcaat taaacgccac ggacaatctc gtggacctat gtcacacggt tctcatttcc
     1561 acagagcacc aggttctgta ggtatggctt cagatgcttc tagagtattt aaaggccaaa
     1621 aaatgccagg acgtatgggt ggaaacactg taactgttca aaacttagaa gtagttcaag
     1681 ttgacacaga aaacaaagtt atcttagtaa aaggtaacgt acctggacct aaaaaaggtt
     1741 tagtagaaat cagaacttca attaaaaaag gtaataaata ataagaagtg aaaggaggaa
     1801 atgcataatg gctaattatg atgttttaaa attagacgga actaaatcag gttcaatcga
     1861 attaagcgat gcagtattcg gtattgagcc aaataatagc gttttattcg aagctattaa
     1921 tttacaacgt gcttcattac gtcaaggtac gcatgctgtt aagaatcgtt cagcagtaag
     1981 cggtggcgga cgtaaaccat ggaagcaaaa aggaacaggt cgtgctcgtc aaggtacaat
     2041 ccgtgctcca caatggcgtg gcggtggtat cgtattcgga ccaactccaa gaagttatgc
     2101 atacaaaatg cctaagaaaa tgcgtcgttt agctttacgc tcagcattat ctttcaaagc
     2161 tcaagagaat ggcttaactg tagttgacgc attcaacttc gaagctccaa aaactaaaga
     2221 attcaaaaat gtattatcta cattagaaca acctaaaaaa gtattagtag ttactgaaaa
     2281 cgaagatgta aatgttgaat tatcagcacg caacatccct ggcgttcaag tgacaactgc
     2341 tcaaggttta aatgttttag atatcactaa tgctgacagc ttagtaatta ctgaagctgc
     2401 tgctaaaaaa gttgaggagg tgctcggata atggaagcaa gagatattct taagcgcccc
     2461 gtaatcactg agaaatcttc tgaagcaatg gctgaagaca aatacacttt cgacgttgat
     2521 actcgtgtta acaaaacaca agtaaaaatg gctgttgaag aaatcttcaa cgtaaaagtt
     2581 gcaagtgtta atatcatgaa ttacaaacct aagaaaaaac gtatgggccg ttaccaaggc
     2641 tatacaaaca aaagaagaaa agcgattgta actcttaaag aaggatcaat cgacttattt
     2701 aactaataaa acaattacca ttaaggaggt aagcgacaat ggctattaaa aagtataagc
     2761 caataacaaa tggtcgtcgt aatatgactt cgttagattt cgcagaaatc acgaaaacta
     2821 cacctgaaaa gtcattatta aaaccgctac cgaaaaaagc gggacgtaac aaccaaggta
     2881 aattgactgt aagacaccat ggtggtggac acaaacgtca ataccgtgtt atcgatttca
     2941 aacgtaacaa agatggtatc aatgcaaaag ttgattctat tcaatatgat ccaaaccgct
     3001 cagcaaacat cgctttagtt gtatatgcag acggtgaaaa acgatatatc attgctccta
     3061 aaggattaga agtaggtcaa atcgttgaaa gtggtgctga agctgacatc aaagttggta
     3121 acgcattacc attacaaaac attccagttg gtacagtagt acacaacatc gagcttaaac
     3181 ctggtaaagg tggacaaatc gctcgttcag ctggtgcaag tgctcaagta cttggtaaag
     3241 aaggtaaata cgtattaatc agattaagat ctggtgaagt tcgtatgatc ttatctactt
     3301 gccgtgctac aatcggtcaa gttggtaacc tacaacacga attagttaac gttggtaaag
     3361 ccggacgttc aagatggaaa ggtatccgtc caacagttcg tggttctgta atgaacccta
     3421 acgatcaccc acacggtggt ggtgaaggtc gtgctcctat cggtagacca tctccaatgt
     3481 caccatgggg taaacctacg cttggtaaga aaactcgtcg tggtaaaaaa tcatcagaca
     3541 aacttatcgt tcgtggacgt aagaaaaaat aatataatca acttatttgg gtgtgcggct
     3601 taaagctgca cgcacataat aagaagggag gcgcccaaat ggctcgtagt attaaaaaag
     3661 gacctttcgt cgatgagcat ttaatgaaaa aagttgaagc tcaagaagga agcgaaaaga
     3721 aacaagtaat caaaacatgg tcacgtcgtt ctacaatttt ccctaatttc atcggacata
     3781 cttttgcagt atacgacgga cgtaaacacg tacctgtata tgtaactgaa gatatggtag
     3841 gtcataaatt aggtgagttt gctcctactc gtacattcaa aggacacgtt gcagacgaca
     3901 agaaaacaag aagataatat ctattaagta gaggaggaca tcctaatgga agcaaaagcg
     3961 gttgctagaa caataagaat cgcacctcgt aaagtaagac tagttcttga cttaatcaga
     4021 ggtaaaaatg ctgctgaagc tattgcaatt ttaaaattaa caaacaaagc ttcatcacca
     4081 gtaattgaaa aagtattaat gtccgcttta gctaatgctg aacataacta tgacatgaac
     4141 acagatgaat tagtagttaa agaagcatat gctaacgaag gaccaacatt aaaacgtttc
     4201 cgtccacgtg cgcaaggtcg tgcaagtgcg attaacaaac gtacaagcca cattacaatc
     4261 gtcgtaagtg acggtaaaga agaagctaaa gaagcttaat taacttttaa ggagggaata
     4321 ctgtgggtca aaaaattaat ccaatcggac ttcgtgttgg tattatccgt gattgggaag
     4381 ctaaatggta tgctgaaaaa gacttcgctt cacttttaca cgaagattta aaaatccgta
     4441 aatttattga taatgaatta aaagaagcat cagtttctca cgtagagatt gaacgtgctg
     4501 caaaccgtat caacattgca attcatactg gtaaacctgg tatggtaatt ggtaaaggcg
     4561 gttcagaaat cgaaaaatta cgcaacaaat taaatgcgtt aactgataaa aaagtacaca
     4621 tcaacgtaat tgaaatcaaa aaagttgatc ttgacgctcg tttagtagct gaaaacatcg
     4681 cacgtcaatt agaaaaccgt gcttcattcc gtcgtgtaca aaaacaagca atcactagag
     4741 ctatgaaact tggtgctaaa ggtatcaaaa ctcaagtatc tggtcgttta ggcggagctg
     4801 acatcgctcg tgctgaacaa tattcagaag gaactgttcc acttcatacg ttacgtgctg
     4861 acatcgatta tgcacacgct gaagctgaca ctacttacgg taaattaggc gttaaagtat
     4921 ggatctatcg tggagaagtt cttcctacta agaacactag tggaggagga aaataataat
     4981 gttactacca aaacgtgtaa aatatcgtcg tcaacatcgt cctaaaacaa ctggtcgttc
     5041 taaaggcggt aactacgtaa catttggtga gtttggttta caagctacaa caacgtcttg
     5101 gatcacatct cgtcaaatcg aatctgctcg tatagcaatg acacgttaca tgaaacgtgg
     5161 cgggaaagtt tggattaaaa tcttcccaca tacaccatat actaaaaaac ctttagaagt
     5221 acgtatgggt gctggtaaag gtgcggttga aggctggatc gcagttgtta aaccaggtag
     5281 aattttattc gaagttgctg gcgtttctga agaagttgcg cgtgaagcac tacgtttagc
     5341 aagtcacaaa cttccagtaa aaactaagtt tgtaaaacgt gaggaattgg gtggtgaaac
     5401 aaatgaaagc taaggaaatt agagacttaa ccacttcaga aatcgaagaa caaatcaaat
     5461 cttcaaaaga agagcttttt aacctacgct ttcagttagc tacaggtcaa ttagaagaaa
     5521 ctgcacgtat tcgtacagta agaaaaacga ttgcacgtct aaaaactgtt gctcgtgaaa
     5581 gagaaattga acaaagtaag gctaatcaat aattaagtaa gaggaggtta caaaagtgag
     5641 cgaaagaaac gatcgtaaag tttatgtagg taaagttgtt tcagacaaaa tggacaagac
     5701 tattacagta cttgttgaaa cttacaaaac acacaaatta tacggtaaac gagtaaaata
     5761 ctctaaaaaa tacaaaactc atgatgaaaa caattcagct aaattaggag acattgttaa
     5821 aattcaagaa actcgtcctt tatcagcaac aaaacgtttt cgtttagtag agattgttga
     5881 agagtcagta attatttaat acaagtttag agataaggga ggtttaacta atgatccaac
     5941 aagaaacacg cttgaaagta gcagacaact ctggtgctcg tgaagttctt acaatcaaag
     6001 tattaggtgg atctggtcgt aaaacagcaa acatcggcga tgttatcgta tgtactgtta
     6061 aaaatgcaac accaggtggc gttgttaaaa aaggtgacgt tgtcaaagct gtaatcgtac
     6121 gtactaagtc aggtgttcgt cgtaatgacg gttcatacat caaatttgat gaaaatgcat
     6181 gtgttatcat ccgtgatgac aaaggcccac gtggtactcg tatcttcgga cctgttgctc
     6241 gtgaattacg tgaaggtaac ttcatgaaaa tcgtatcatt agcaccagaa gtactttaat
     6301 taataacaac caatacaaat aaggaggtgc ccacatgcat atcaaaaaag gtgacaacgt
     6361 taaagttatc gcaggtaaag acaaaggtaa agaaggtaaa gtaattgcta ctctacctaa
     6421 aaaagaccgt gtcgttgtgg aaggtgttaa cattatgaaa aaacaccaaa aaccaactca
     6481 attaaatcct gaaggtggaa tcttagaaac agaggcagca atccatgttt ctaatgtaca
     6541 attattggac cctaaaacaa acgaaccaac tcgtgtaggt tacaaatttg ttgatggtaa
     6601 aaaagttcgt atcgctaaaa aatctggcga agaaattaaa tctaataatt aataacaagt
     6661 tgaaaggagg atcccacttt gaaccgttta aaagaaaagt ttaacactga agttactgaa
     6721 aacttaatga aaaaattcaa ttatagttca gtaatggaag taccaaaaat agataaaatc
     6781 gttgtgaaca tgggtgtagg tgacgcagta caaaattcta aagtattaga caatgctgtt
     6841 gaagaattag aattgatcac tggtcaaaaa ccattagtaa ctaaagctaa aaaatcaatc
     6901 gcgactttcc gtttacgtga aggtatgcca atcggtgcga aagtaacact tcgcggtgaa
     6961 agaatgtatg aattcttaga caaattaatt tcagtatcat taccacgtgt acgtgacttc
     7021 caaggtgttt ctaaaaaagc atttgacgga cgcggtaact acactttagg tgttaaagaa
     7081 caattaattt tcccagaaat cgactatgat aaagtaagta aagttagagg aatggatatt
     7141 gttatcgtaa cgactgctaa cactgatgaa gaagctcgtg aattgttagc taacttcggt
     7201 atgccattcc gtaaataatt atttaaagga ggctaattaa gtggctaaaa cttcaatggt
     7261 tgctaagcaa caaaaaaaac aaaaatatgc agttcgtgaa tacactcgtt gtgaacgttg
     7321 tggccgtcca cattctgtat atcgtaaatt taaattatgc cgtatttgtt tccgtgaatt
     7381 agcttacaaa ggccaaatcc ctggcgttcg taaagctagc tggtaataaa aaagagtctg
     7441 aaaggaggca acaatcaatg acaatgacag atccaatcgc agatatgctt actcgtgtaa
     7501 gaaacgcaaa catggtgcgt cacgagaagt tagaattacc tgcatcaaat attaaaaaag
     7561 aaattgctga aatcttaaag agtgaaggtt tcattaaaaa tgttgaatac gtagaagatg
     7621 ataaacaagg tgtacttcgt ttattcttaa aatatggtca aaacgatgag cgtgttatca
     7681 caggattaaa acgtatttca aaaccaggtt tacgtgttta tgcaaaagct agcgaaatgc
     7741 ctaaagtatt aaatggttta ggtattgcat tagtatcaac ttctgaaggt gtaatcactg
     7801 acaaagaagc aagaaaacgt aatgttggtg gagaaattat cgcatacgtt tggtaataaa
     7861 aaataaggag gtgccataac atgagtcgtg ttggtaagaa aattattgac atccctagtg
     7921 acgtaacagt aacttttgat ggaaatcatg taactgttaa aggtcctaaa ggtgaattat
     7981 caagaacttt aaatgaaaga atgacattca aacaagaaga aaacacaatt gaagttgtaa
     8041 gaccatctga ttctaaagaa gatagaacaa accatggtac aactcgtgct ttattaaaca
     8101 atatggtaca aggtgtttct caaggatacg taaaagtact tgaacttgtt ggtgtaggtt
     8161 accgtgctca aatgcaaggt aaagacttaa tccttaacgt tggttattct cacccagtag
     8221 aaattaaagc tgaagaaaac attactttct cagttgagaa aaacacagtc gttaaagttg
     8281 aaggtatttc aaaagaacaa gttggagcat tagcatctaa catccgttca gtaagacctc
     8341 cagagcctta caaaggtaaa ggtattcgtt accaaggtga atacgttcgc cgtaaagaag
     8401 gtaaaactgg taaataatag ataaactcta aagaaaggag tattgaaatg atcagtaaaa
     8461 ttgataaaaa taaagtgcgt ttaaaaagac atgctcgtgt tcgtactaac ttatcaggta
     8521 cagctgaaaa gccacgttta aacgtatatc gttcaaacaa gcatatctac gctcaaatta
     8581 ttgatgataa taaaggcgta acattagctc aagcttcttc aaaagacagc gacattgcta
     8641 ctacagcaac taaagttgaa ttagcaacta aagtcggtga agcaattgct aaaaaagctg
     8701 ctgacaaagg cattaaagaa atcgtatttg accgtggagg atatttatat cacggacgtg
     8761 ttaaagcatt agctgaagca gcaagagaaa gcggattaga attttaattt aaaggaggga
     8821 caaatacatg gctcgtagag aagaagagac gaaagaattt gaagaacgcg ttgttacaat
     8881 caaccgtgta gcaaaagttg taaaaggtgg tcgtcgtttc cgtttcactg cattagttgt
     8941 agttggagac aaaaatggtc gtgtaggttt cggtactggt aaagctcaag aggtaccaga
     9001 agcaatcaaa aaagctgttg aagcagctaa aaaagattta gtagttgttc cacgtgttga
     9061 aggtacaact ccacacacaa ttactggccg ttacggttca ggaagcgtat ttatgaaacc
     9121 ggctgcacct ggtacaggag ttatcgctgg tggtcctgtt cgtgccgtac ttgaattagc
     9181 aggtatcact gatatcttaa gtaaatcatt aggatcaaac acaccaatca acatggttcg
     9241 tgctacaatc gatggtttac aaaaccttaa aaatgctgaa gatgttgcga aattacgtgg
     9301 caaaacagta gaagaattat acaattaagg agggaaaact agttatggct aaattacaaa
     9361 ttaccctcac tcgtagtgtt attggtcgtc ctgaaacaca acgtaaaact gttgaagctt
     9421 taggtcttaa aaagactaac agttcagtag ttgttgaaga taaccctgct attcgtgggc
     9481 aaatcaacaa agttaagcac ttagtaacag tagaagaaaa ataatcaagg aggtgccgaa
     9541 atgaaattac atgagttaaa accggcagaa ggttcacgta aagaacgcaa tcgtgttgga
     9601 cgtggtgttg cgacaggtaa cggtaaaaca agtggtcgcg gacacaaagg tcaaaaagct
     9661 cgttcaggcg gtggtgtaag accaggattt gaaggtggtc aattaccatt attccgtcgt
     9721 ttaccaaaac gtggttttac taacataaat cgtaaagaat atgctattgt taacttagac
     9781 caacttaata aatttgaaga tggtactgaa gtaactccag ctttattagt agaatctggt
     9841 gttgttaaga atgaaaaatc tggtatcaaa atactaggta atggttcact tgataagaaa
     9901 ttgacagtga aagctcataa attctcagct tcagcagcag aagctattga tgctaaaggt
     9961 ggagcacacg aggtgatcta atgattcaaa cccttgtgaa cttctttaga acaaaagagg
    10021 ttcgtaacaa gattttcttc acactagcaa tgttagtaat ttttaaaata gggacttata
    10081 taccagctcc aggagtaaat cctgcagctt ttgataatcc ccaaggttct caaggtgcca
    10141 ctgagttatt aaatactttt ggtggcggag ccttgaaacg attttctatt tttgcaatgg
    10201 gtattgtacc ctacatcact gcatcaatcg taatgcaatt attacaaatg gatattgtcc
    10261 ctaaattctc agaatgggca aaacaaggtg aagtaggtag aagaaagtta aataacgtta
    10321 ctcgttattt agcaatttct ttagcattta tccaatctat aggtatggca ttccaattta
    10381 ataattatct caaaggtgcg ctgattatca atcagtcaat tatgagttat ttattaatag
    10441 cactagtttt gacagcagga actgctttct taatatggct tggtgatcaa atcactcagt
    10501 tcggtgttgg taatggtatt tctattatca tatttgcggg tattttatca acgttgccag
    10561 ccagtctaat tcaatttgga caaactgcgt tcgttgggca agaagataca tcattagcat
    10621 ggttgaaagt attaggactt ttagtgtcac tgatactatt aactgtaggt gctatttatg
    10681 tacttgaagc cgttcgtaaa attccgattc aatatgctaa gaaacaaact gctcaaagat
    10741 taggttcaca ggcaacttat ctacctttaa aagtgaactc agccggtgtt attcctgtaa
    10801 tctttgcaat ggcgttcttc ttactgccta gaacattaac gttgttctat ccagataaag
    10861 aatgggcaca aaacattgcg aatgctgcaa atccttcaag taatgttggt atggttgtat
    10921 atattgtact aattatacta ttcacatact tctatgcatt tgtacaagtt aatccggaaa
    10981 aaatggctga taaccttaag aaacaaggta gctatgttcc aggtattaga cctggtgaac
    11041 aaaccaaaaa atatattact aaagttttat atcgtttaac ttttgtaggt tctatcttct
    11101 tagccgttat ttcaattcta ccgatattag caactaagtt tatgggatta ccacaatcaa
    11161 ttcagattgg tggtacaagt ttacttatcg ttattggtgt agcgattgaa acaatgaaat
    11221 ctttagaagc tcaagtatct caaaaagaat ataaaggctt tggtggtaga taattttagg
    11281 agggcactta tgaatatcat tttgatgggt ttacctggcg caggtaaagg aactcaagca
    11341 agtgaaattg tcaagaaatt cccaataccc cacatttcaa ctggtgacat gttcagaaaa
    11401 gctataaaag aagaaactga attaggtaaa gaagctaagt cttatatgga ccgtggcgaa
    11461 ttagttcctg atgaagtgac tgtaggtatc gttaaggaaa gaatttctga agacgatgca
    11521 aaaaaaggct ttttattaga tggcttccca agaacaatcg agcaagctga ggcattaaat
    11581 aatattatgt ctgagcttga cagaaacatt gatgctgtca tcaatatcga agttccggaa
    11641 gaagaattaa tgaaccgtct tacaggtcgt cgaatctgtg agtcatgtgg tacaacgtat
    11701 catcttgtat ttaatcctcc gaaggtcgaa ggtatttgtg atatcgatgg tggtaaattg
    11761 tatcaacgag aagatgataa tcctgaaacg gtagctaatc gtttgagtgt taatattaaa
    11821 caatctaagc ctattttaga tttctatgat caaaaaggtg tattgaaaaa tattgatggt
    11881 tcaaaagata ttagcgacgt taccaaagat gtcattgata ttttagatca tttgtaatag
    11941 atcaactttt agcggtctat tttaagtgag gcatgattta atcatattag atagatgaga
    12001 gtggcaatta acgataatta attcccgtaa tttttgttta acttataaaa ttaatcactg
    12061 atagattcgt aacaataaag ttgtctattg acgattacct tactattgca aaaaggggga
    12121 agttaatcaa tggctaaaca agatgtaatt gaattagaag gtactgtatt agatacttta
    12181 ccgaacgcaa tgtttaaagt agaattagaa aatggtcatg agattttagc tcacgtaagt
    12241 ggtaaaatca gaatgaatta cattcgtatt ctacctggcg acaaagtaac tgttgagatg
    12301 tctccgtacg atttaacacg cggaagaatt acttatcgtt ataaataatc gtcactccat
    12361 aatataggga ggtataaaaa tgaaagtaag accatcagta aaacctattt gcgaaaaatg
    12421 taaagtcatt aaacgtaaag gtaaagtaat ggtaatttgt gaaaatccaa aacacaaaca
    12481 aagacaaggt taataaaaga gaggtgtaaa ttaatatggc acgtattgca ggagtagata
    12541 ttccacgtga aaaacgcgta gttatctcat taacttatat atacggtatc ggtacgtcaa
    12601 ctgctcaaaa aattcttgaa gaagctaacg tatcagctga tactcgtgtg aaagatttaa
    12661 ctgatgacga attaggtcgc atccgtgaag ttgtagacgg ttataaagtc gaaggtgact
    12721 tacgtcgtga aactaactta aatatcaaac gtttaatgga aatttcatca taccgtggta
    12781 tccgtcaccg tcgtggttta ccagttcgtg gtcaaaaaac gaaaaacaac gcgcgtactc
    12841 gtaaaggacc agttaaaacg gtagctaaca agaaaaaata ataggtaaag gaggcaaatt
    12901 ttaaatggca cgtaaacaag tatctcgtaa acgtagagtg aaaaagaata ttgaaaatgg
    12961 tgtagcacac atccgttcaa cattcaacaa cactattgta actatcactg atgagttcgg
    13021 taatgcttta tcatggtcat cagctggtgc attaggattc aaaggatcta aaaaatcaac
    13081 accatttgca gcacaaatgg cttctgaaac tgcatctaaa tcagctatgg agcatggttt
    13141 aaaaacagtt gaagtaacag ttaaaggacc tggtccaggt cgtgaatcag ctattcgtgc
    13201 attacaatct gcaggtttag aagtaactgc gatcagagac gttactccag tacctcataa
    13261 cggttgtcgt ccaccaaaac gtcgtcgtgt ataatttatg atggtattgt tacaggtcac
    13321 tgagcaaaca ttttaaatta agtcgacgta tataaggagg atatttaaat gatagaaatc
    13381 gaaaaaccta gaattgagac aattgaaatt agtgaagatg ctaaattcgg taagttcgtt
    13441 gttgaaccac tagaacgtgg ctacggtact acactaggaa actccttacg tcgtatccta
    13501 ctatcttcat taccaggtgc agccgttaag tatattgaaa ttgagggagt tttacatgaa
    13561 ttctcagcag tagacaatgt agttgaagat gtttctacaa ttattatgaa cattaaacaa
    13621 ttagcattga aaatttactc tgaagaagat aaaactttag aaattgatgt acgtgatgaa
    13681 ggcgaagtaa cagcaagcga cattacacat gatagtgatg ttgaaatttt aaacccagag
    13741 cttaaaattg caacagtatc taaaggtggt cacttaaaaa ttcgtctagt tgctaacaag
    13801 ggtagaggtt acgcattagc agaacaaaat aatactagtg atttaccaat tggtgtaatc
    13861 cctgttgatt cattgtattc acctgttgaa cgtgtgaact atactgttga aaatacacgt
    13921 gtaggtcaaa gcagtgattt tgataaatta acattggatg tttggactaa tggttcaatc
    13981 acaccacaag aatcagtttc attagcagca aaaataatga ctgaacactt gaatatcttc
    14041 gttggtctta ctgatgaagc gcaaaacgct gaaatcatga ttgaaaaaga agaagatcaa
    14101 aaagaaaaag tattagaaat gtctattgaa gaattagact tatctgtacg ttcatataac
    14161 tgcttaaaac gcgcaggaat caattctgtt caagagttag ctgacaaatc tgaagctgac
    14221 atgatgaaag tgcgtaattt aggtcgtaaa tctttagaag aagttaaata caaattagaa
    14281 gatttaggat taggattaag aaaagaagat tgataaagga ggttaactca tgggttacag
    14341 aaaattaggt cgtacttctg atcaacgtaa agctatgtta cgtgacttag ctacatcact
    14401 tattattagt gaacgtattg aaactacaga agctcgtgca aaagaagttc gcagtgttgt
    14461 tgagaaatta atcactttag gtaaaaaagg agatttagct tctcgtcgta atgcagctaa
    14521 aactttacgt aatgttgaaa tcttaaacga agatgaaact acacaaactg cacttcaaaa
    14581 attatttggt gaaatcgcag agcgttacac agaacgtcaa ggtggttaca ctcgtatcct
    14641 taaacaaggc cctcgtcgcg gtgacggtgc tgaatcagta attatcgaat tagtataaga
    14701 atttatataa atacactaac tacttttata taaagcaaat gagtgcaacg ataatgtttg
    14761 ccacatacaa atattgtcta gctcagagta ccccatcaac taaataaaga tttaaagcgt
    14821 gaactcaaca aacttatgat ggggtgcgcg cttttttatt gaataagata tcggacttat
    14881 gaacagaaat gtttttaagt ccgatttctt tatatctact tttttgagtg cgaatctaaa
    14941 aatatgatca agctagtttg attaatttca ctagaactgt atgaagtgaa agtattatcg
    15001 ttgaatcaaa cgcggccaac cctatgttag taaatcattt attttattaa cgtttataca
    15061 taattgttag agagggataa atgtccttat cattagttgt taaacaacac tttaaagact
    15121 agaaatgagt cagaaaatac tagtaatata tagagaaccc agtcaatgtc atatacagcg
    15181 atttataaat attgtaaaat atgatagata cacgaatcgt aaaggagaga gacaagtgga
    15241 ggataagaat tcagttattg tatttaaaaa tgtttcattt caatatcaaa gtgatgcatc
    15301 cttcacattg aaagatgttt cttttagtat acctaaaggt cagtggacat ctattgttgg
    15361 tcataacggt tctggaaaat ctacaattgc caagttaatg attggcatag agaaagttaa
    15421 atctggagaa attttttata ataatcaaac tataacagat gataattttg aaaagttaag
    15481 aaaagacata ggaatagtat tccagaatcc ggataatcaa tttgttggtt caattgtaaa
    15541 atacgatgtg gcatttggac tcgaaaatca tgcggttcca catgacgaaa tgcatagaag
    15601 agtcggcgaa gcacttaaac aagttgatat gttagaacgt gcagattatg aacctaatgc
    15661 attatcgggg ggacagaagc agcgtgtggc tatagcaagt gtattagcac ttaacccctc
    15721 tgtcattata ttagatgagg cgacttctat gttagatcct gatgcacgtc aaaatttatt
    15781 ggatttagtg agaaaagtta aatcagaaca taatattaca atcatttcta ttacgcatga
    15841 tttatctgag gcgatggaag cagatcatgt tatcgttatg aataaaggga ctgtctataa
    15901 agaaggcaca gcgattgaaa ttttcgacca tgcagaagag ttaacaacaa taggtctaga
    15961 tttgccattc ccaatcaaaa taaatcaaat gctgggatac caaacatcat tcttaactta
    16021 tgaagggctg gtggatcaac tatgactata cgctttgaca atgtaagtta tacctatcaa
    16081 aaagggacac cataccagca tcaagctatt catgatgtta atacagaatt tgaacaaggt
    16141 aaatattacg ccatcgttgg acaaacgggt agtggtaaat caacattgat acaaaatatt
    16201 aatgcactgt taaagccaac tactgggaca gttacagttg atgacattac tattacacat
    16261 aagaccaaag ataaatatat tagacctgta agaaaaagaa ttggaatggt atttcaattt
    16321 cccgaatctc aattatttga ggacacagta gagcgtgaaa tgatatttgg acctaaaaac
    16381 tttaaaatga atttagatga agccaaaaac tatgcccatc gtctgttgat ggatttaggc
    16441 ttttcaagag atgtaatgtc acaatcacca tttcaaatgt caggtggaca aatgcgtaaa
    16501 atagcgattg tatcgatatt ggcaatgaat cctgatatta tcgtggttga tgaacccaca
    16561 gcaggacttg atccacaaag taaacgacaa gtaatgcgat tactaaagtc attacaaaca
    16621 gatgaaaata aggcaattat cctaatttca catgatatga atgaagtcgc gcgttatgca
    16681 gatgaagtca tcgttatgaa agaaggtagt atagtgtcgc aaacatcacc aaaagagctc
    16741 ttcaaagata aaaaaaaatt ggcggattgg catattggtt tgccagaaat tgttcagtta
    16801 caatatgact ttgaacaaaa atatcaaaca aaattaaaag atattgcctt aactgaagaa
    16861 gcatttgtaa gcttgtataa ggagtggcaa catgaaaaat aaattgatta tagggcgata
    16921 tttaccaatt aattcttttg ttcatcatct tgatccaaga gcaaagttaa tgtttgtttt
    16981 tctttttatt atattaatat ttttctgtca ttcaccatta acatatcttt gggtgtttgc
    17041 acttatctta ttcattatga gattagcaaa aattcaatta tggttcctaa tcaaaggttt
    17101 aacaccaata tttttctttt taatcttcac attaatgatg catatatttt taactaaagg
    17161 tggatatgta ttagttgaat ggcatggcat cacgattgaa actaatggta ttttagaagg
    17221 gctttatata tcattgcgtt taattgggat tgtgatgatt gcaacaatta tgacactatc
    17281 tacaagtcca attgatttaa cagatgcgtt tgaaagatta cttgcaccac taaagatgtt
    17341 taaattacca gttcatcaat taagtatgat aatgtccatt gcgttacgat tcatcccgac
    17401 gttaatggat gagttagata aaattatttt ggcacaaaag tcgcgtggtt ctgaaataag
    17461 ttcagggaac attgcaacac gtattaaatc atttattcca ctactagtgc cactattcat
    17521 ctcagctttc caacgcgccg aagaattagc ggtcgcaatg gaagttaggg gttatgatgc
    17581 caatgttaag agaacgagct acagacagct taaatggcaa ttgagagata cgttatcttt
    17641 aatcatgatt atcccaattg ctattatttt attcgtatta aaatattcag gagtgtaaat
    17701 tcatgcgtat attagtagaa attgcgtatc aaggaaataa ttttctaggt tttcaaattc
    17761 aacaaaatgg tcgaacggta cagcaacaat ttgaaaagct attacaacgc atgcataaaa
    17821 gacatgtgag aatccatcct tcaagtagga ctgatagagg tgtacatgcg atacagcaat
    17881 actttcattt cgacacagag ctgaatatac ctatgtcaca atggcaatat gcaatgaacc
    17941 gaacgttacc tgatgatatt tatgtcaaca atgtagttac agtcgatgac gattttcatt
    18001 gtcgttacga ttgtgtaggt aaacgctatc gatataaagt atatcaagca caacaccgcg
    18061 atccatttca aagtggtttg aaaacattta ttcctgaaac gttggattta gataaaatga
    18121 acagagcggc acaacaattc ataggtacac atgattttac gggattttgt tcgcaaaaaa
    18181 ctgaagtaga aagcaaggtt agaacattat accaaagtga aattgtaaag acagatgatg
    18241 gctttgatta tattgtcact ggatctggat ttttatataa catggtgcgt gtcttagttg
    18301 catttttaat agaagttgga aaaggacgac atgaaatttc tgatgtgccg aagctacttg
    18361 aatcaaagaa tcgtaagaat gttcctttca ctgcaacggc tgaaggatta tatttagaga
    18421 agatttattt agatgaaaat gagttattaa aagactttgg caacgatatc aaaatacatc
    18481 gtaaaaaatc attacaaaat gactgattgc cattgacaaa aagcttgtga aattataaga
    18541 ttattaacgg tattgtttta tatccacccc acgataagcc ccggaaactt attgtgttac
    18601 aagatatata agcagaaacg aacaacagtt aacaaaataa atgaaattaa acgttttaaa
    18661 aatgaaacaa atgaaatcat ctattaggtt atgaaactgt ttatagcttg aatagaagca
    18721 tttatttttt aggaggacaa ttattatgcg tcaaacattt atggcaaatg aatcaaacat
    18781 tgagcgcaaa tggtatgtta tcgatgctga aggccaaaca ttaggtcgtt tatcatcaga
    18841 agtagcatct atcttacgcg gtaaaaataa agtaacttac acaccacacg ttgatactgg
    18901 tgattatgta atcgttatta atgcatcaaa aatcgaattt actggtaaca aagaaactga
    18961 caaagtttac taccgtcact caaatcaccc aggtggtatc aaatcaatca ctgctggtga
    19021 attaagaaga actaacccag aacgtttaat tgaaaactca attaaaggta tgttaccaag
    19081 cactcgttta ggcgaaaaac aaggtaaaaa attatttgta tatggtggcg ctgaacatcc
    19141 acacgctgca caacaaccag aaaactacga attacgtggt taattagaag gaggaaatga
    19201 ctttggcaca agttgaatat agaggcacag gccgtcgtaa aaactcagta gcacgtgtac
    19261 gtttagtacc aggtgaaggt aacatcacag ttaataaccg tgacgtacgc gaatacttac
    19321 cattcgaatc attaatttta gacttaaacc aaccatttga tgtaactgaa actaaaggta
    19381 actatgatgt tttagttaac gttcatggtg gtggtttcac tggacaagct caagctatcc
    19441 gtcacggaat cgctcgtgca ttattagaag cagatcctga atacagaggt tctttaaaac
    19501 gcgctggatt acttactcgt gacccacgta tgaaagaacg taaaaaacca ggtcttaaag
    19561 cagctcgtcg ttcacctcaa ttctcaaaac gttaattgtc ggacgatata tacaaaacac
    19621 ctcgaagtta tgtcgaggtg tttttttacg tttgtgtggg gaatatggaa cgtgtagagt
    19681 ataatgaatt ttcctttatt taatgtctca ggaatagaat aaattcaact atgtattagt
    19741 gatttgcacg tatgatttgt acatgcttaa acaatcaaaa gtgtccatta ttaaattatc
    19801 atttagcttg cggaggccgt atgataaccg gacgattgtg ttctaatcat agaggctcaa
    19861 tggaagaact acgggacgaa aaccagacga taactcaaga accatgattt aagagtatta
    19921 tcaaaataag gagtgactta attgaaagat tttatatttt ggaagaaagc ttttattcct
    19981 gtttatttta ttgttgcgat gctggtgttt ctacttttta ggttttatat taaaacagac
    20041 aacttttcta tgtatttaat gattatcttc ttaatttgtt taggaactgc atctatcatt
    20101 tataactata aaaacaatcg ataaatataa gtatgaaatg tataagtcac agttagatct
    20161 aagtcttgct gtggcttttt ttatcgaaat gacaatttta aatcgccaaa aacaacattt
    20221 tcagtccgcc ataaaacaac aatttcaaat cgccattgac taaacacgac atccaaaaat
    20281 tattgtaaca attcttcaca tcaatcaatc ctccatcact cataatcact ccctacaaaa
    20341 tctactcatg catttttgga atacttagta ttacaaataa cgatttttat tcatcttaca
    20401 aaggttatat aatgtaatga aggcaatttt tatgtattgc aaatctaatc gtacatgtaa
    20461 aattttgata aacatcatta attttgcgta actatcatta gattacaaat cacaatgtaa
    20521 ttacatgtaa tacacatcta tacatcacat ttgaagggaa atgaatataa atgactgata
    20581 aaaagtacac tgcagccgat atggttattg atactttgaa aaataatggg gtagaatatg
    20641 tttttggtat tccgggtgca aagattgact atctatttaa tgctttaatt gatgatggtc
    20701 ctgaacttat tgtcacgcgt catgaacaaa acgccgcaat gatggcgcaa ggtattggaa
    20761 gattaacagg caaaccgggt gtagtacttg ttacaagtgg gcctggtgta agtaatttaa
    20821 cgactggact attaacagct acatctgaag gggatcctgt cttagcgtta ggcggccaag
    20881 tgaaacgtaa tgatttatta cgattaacgc atcaaagtat tgataatgct gctttattaa
    20941 aatattcatc aaaatatagt gaagaagtac aagatcctga atcattatca gaagttatga
    21001 caaatgcaat tcgaattgct acttcaggga aaaatggcgc aagttttatt agtattccgc
    21061 aagatgtgat ttcttcacca gttgaatcta aggcgatatc actttgtcaa aaaccaaatt
    21121 taggtgtacc aagtgaacag gatattaatg atgtcattga agcgattaaa aatgcatcat
    21181 ttccggtttt actagctgga atgagaagtt caagtgcaga cgaaacgaat gccattcgca
    21241 aattagtaga gcgtaccaat ttacccgttg tagaaacatt ccaaggtgcg ggtgtgatta
    21301 gtcgtgaatt agaaaatcat ttctttggcc gagtgggttt attccgtaat caagttggtg
    21361 atgaattatt acgtaaaagt gatttagttg ttacaatcgg ttatgatcct atagaatatg
    21421 aggctagtaa ctggaataaa gaattagata cgcaaataat caatattgac gaagttcaag
    21481 ctgaaattac taattatatg caaccgaaaa aagagttgat tggtaatatt gctaaaacga
    21541 ttgaaatgat ttctgacaaa gtagatgaac catttataaa tcaacaacat ttggatgaat
    21601 tagaacagtt aagagcgcat attgatgaag aaactggtat taaagcgacg catgaagaag
    21661 gcatcttaca cccagttgaa attatagaat ctatgcaaaa ggtattaact gatgaaacaa
    21721 cagtgacagt tgatgttggt agtcactata tttggatggc acgtaatttc agaagttaca
    21781 atccaagaca tttattattt agtaacggta tgcaaacgct tggtgtagca ttaccgtggg
    21841 caatttcagc tgcacttgta cgccctaata cgcaagttgt atccgttgct ggcgatggag
    21901 gctttttatt ttcatcacaa gatttagaaa cggccgtacg taaaaatcta aacattatcc
    21961 aacttatttg gaatgatgga aaatataata tggttgaatt ccaagaagaa atgaaatata
    22021 aacgttcatc aggcgtagac ttcggtcctg tagatttcgt aaaatatgct gaatcatttg
    22081 gcgcaaaagg tttacgagtt actaatcaag aagaattaga agcggcaatt aaagaggggt
    22141 atgaaacaga tggcccagta ttaattgata tacctgtaaa ctacaaagat aatatcaaac
    22201 tttcaacaaa tatgttacct gacgtattta actaaaacaa agataaatgt taatgaggag
    22261 tgggaaattt tatggctagt gttttatatc aacatggtac attaggcaca ttaatggcag
    22321 gattattaaa aggaactgca tcaataaatg aattattgca acatggtgac ctaggtatcg
    22381 ctacactaac aggttcaaat ggtgaggtca tctttttaga tggaaaagct taccatgcaa
    22441 atgaacataa agaatttgta gaattaaaag gtgatgagtt aacaccatat gcaactgtaa
    22501 ctaaattcgt agcagataca agctatgaaa cgaaagataa atcttctgaa gcagtttttg
    22561 cagaaattaa ggaaaagatg ttgagtgaaa atttattttc tgcagtaaaa atttcaggct
    22621 tatttaaaaa aatgcatgta cgtatgatgc cggcacaaga gccaccttat acacgtttaa
    22681 tcgattcagc tagaagacaa cctgagcaaa ctgaaacata cgtcaaaggt tcagtcgttg
    22741 ggttctttac accagaacta ttccatggta tcggatcagc aggattccat gtacactttg
    22801 caaatgatga tcgtaatttt ggtggacatg tcttagattt tgaagtagaa gatgttaagg
    22861 tagaaatcca aaatatagaa acatttgaac agcattttcc aattcaagat gaagatttca
    22921 taaatgcaaa tattgactat aaagatattg cggacgaaat tagagaagct gaataatgaa
    22981 tgcggaaatg taatgacggt ttatgaaaat tgacttcaaa atgcgttatt tggaaatggt
    23041 agttttttta tatgattaag caagactaca atagaacaaa tatatttata attacgtcta
    23101 agtaataaaa taaatccctt cactattagc ggtagtgagg ggatttattt aggttccaga
    23161 tatttgagat ttgctgttat gtttagataa taaattgagg aagacattaa tatagaattt
    23221 actttagtat aggccaatct aatctattgt cagtagaagc aatcaataat aaattacaag
    23281 ttcgatcatt catttcatac taaaaagaca aacgcaattt ttcagcgttt gcctcatgta
    23341 attattcttc attttcaaat tattatttaa ctacaatttg tatctgtttg attgtacttg
    23401 gatcaattga atttttagcg tcgtcatttt tctttagatt cactactttt ttattaccat
    23461 cattcaaagt aagtgtataa gttgcagttt gcgcattatt aattttttct gttgtaacac
    23521 cacgttgtga agctaattca ttttttactt tactgtcaat ttcttgataa agtacctttt
    23581 tattttctgg aaaaacaaag taagttcgat gtaatgcagt aataccatct actgagatcg
    23641 tgtaaggtac agtgtgtttc ccatccattg tcaattcctt ataaccgtta ttactatctg
    23701 cagatgcttc atgactcggt aaagctgcac ctactgttga taataatccc aatgctaaag
    23761 tggcagtaac aaatgatttt agtttcataa ctcaatctcc tttttgtttt gtaggatatt
    23821 acactatttg tataacacgt ttcataaagt ttaaaacat
//
LOCUS       DAMZKC010000024        22945 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.024, whole genome shotgun sequence.
ACCESSION   DAMZKC010000024 DAMZKC010000000
VERSION     DAMZKC010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 22945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22945
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.024"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(143..1180)
                     /gene="mutY"
                     /locus_tag="POB55_000715"
     CDS             complement(143..1180)
                     /gene="mutY"
                     /locus_tag="POB55_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HDI6499700.1"
                     /translation="MYQQSSFKENLIHWFDENQREMPWRQTTNPYYIWLSEVMLQQTQ
                     VKTVIDYYHRFVERFPTVEVLSQASEDEVLKYWEGLGYYSRARNFHTAIKEVYDKYEG
                     LVPKDPDQFKALKGVGPYTQAAVMSIAYNVPLATVDGNVFRVWSRLNDDYRDIKLQST
                     RKSYEQELLPYVTTEAGTFNQAMMELGALICTPKNPLCLFCPVQENCEAFDKGTFEKL
                     PVKSKNVSKKVIEQSVFLIRNNQGQYLLQKRREKLLHGMWQFPMFESEHARRKMTEKI
                     GHDIQPVETPIFELKHQFTHLTWKIKVYAVSGAINIETLPDDMIWFDLSDRDQYTFPV
                     PMSKIYQFING"
     gene            1332..2309
                     /locus_tag="POB55_000716"
     CDS             1332..2309
                     /locus_tag="POB55_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDI6499701.1"
                     /translation="MDTATHIAIGVGLTALATQDPAMASTFGATATTLIVGSLIPDGD
                     TVLKLKDNATYISHHRGITHSIPFTILWPILITFLIFTFFSGTNPFHVWMWAQLAVFL
                     HVFVDIFNSYGTQALRPITNKWIQLSVINTFDPIIFTVLCIGIVLWVIGLHPFAVFFP
                     IIALLIIYYMIRFKMRAVIKQQALKAIQQEHHPVKVFVAPTIKFMEWRVAIQTDAHDY
                     VGKAYGRNVVFSDKVERQTLSTDSILWKVKGNKDIRTFLNFSSIYRWQTTTLADGSTE
                     IRLIDLRYLKNDHYSFVAIAHVTNDNVIDHSYIGWVFTEDKLQRKLYAK"
     gene            complement(2570..3406)
                     /locus_tag="POB55_000717"
     CDS             complement(2570..3406)
                     /locus_tag="POB55_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499702.1"
                     /translation="MLDNIILYFKNLPHTKRYVTERLKQSWKSFLIVLAACLILIIAS
                     ETLFSFSHLTDVKEVRWLFRIIVLIVFAVVMFTIYISYHHYMNDFLVTKLFNISAATP
                     VVIMSILSFIMLVILTMISALVKPVNFETSYIALFYFIVMATIFVGLISVTFGLIRLL
                     TEKINIIFYGVCVLCFLLLPIIFIPNPNHVFINHILMLNPMYYIVNGIAQSIIFGISS
                     MENIPYHFYFILFLCLIAAVNFVLARYTTHAIYNKTSKVTQTDNQQDVSNDSTDEADT
                     SS"
     gene            complement(3415..4932)
                     /locus_tag="POB55_000718"
     CDS             complement(3415..4932)
                     /locus_tag="POB55_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6499703.1"
                     /translation="MGSSIVLKLLKVTHYYRNKQNKKWYLPFGYDAEDIDLNNISLHI
                     YQGEALGIIGEPESSKALVGQLLAGAIKPDKGKVVCTEDLFYGYIEDQSLIHQTVEAY
                     TAQLVQLFPYEINDHKAEQIIQYAHLGDYKTKPVNHISKAAYAQLLLSIARSSKSNII
                     ILNHVIDYLTPQFMERAIELTNDYIENNLTIVSIGDDIDKISQVSNYIAWFSHGQLRM
                     EGSLKQVIPSFKEHERDRLSLNSKEEIENFDLDWKKNRTRIPEMTYNFKRVERYNHAK
                     PPKFLVRFWTLASGTILGLALMMLLIFNNIGIISITDFTNRATMQNENKDPYGEKLAY
                     GIAFNGSVDMQGDKQVTIPKYSVVTITGENSKNYRVTADNKTYYVSKDKLEYFNPAGL
                     YQTHSFKKLAPYMKSNYSNYYAYFNSQLHKKHSSVIKTLVPDDDNRFVASVTQQPIQL
                     LFNDNNQLYGFVYPIVDKKELKDKFNINNNIWITKVGNGYCIANLKEDKWIYIEL"
     gene            complement(4947..5261)
                     /locus_tag="POB55_000719"
     CDS             complement(4947..5261)
                     /locus_tag="POB55_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhH family protein"
                     /protein_id="HDI6499704.1"
                     /translation="MEQKKLSEMSEPELRHEIQLYKEKMRKAEMNGILNEYDVYQSKV
                     IVAESYLVDRKKIEIGKIYKLTDGSNQYFKVERLKGIFAWGFRFNSDEPEEGLPIALL
                     QL"
     gene            complement(5239..6057)
                     /gene="recX"
                     /locus_tag="POB55_000720"
     CDS             complement(5239..6057)
                     /gene="recX"
                     /locus_tag="POB55_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HDI6499705.1"
                     /translation="MPKITKIEVQKKNKERFNLFLDEQFEMGIDIDTLVKFNLKKGQQ
                     LEAADMAEIQKYDHYRIGLNKAIQYLSYKKRTEKEVIQYLQKEEISEQAISEVIEYCY
                     REKLIDHQDYAESLKNTMIRTTDKGPKIYQQKLYQLGIEPNIIEIFTELYREQQELDD
                     IIQIAEKISKTKKGPQNKVKEKVMQSLIQKGFEMETIHAVLNEMDFTQDEAVLDDLLQ
                     RDLEKIYNKNRKKYTQQKLISKTIEGLMRKGYKYDKIKAKLEESGIADGTEEIE"
     gene            complement(6318..7127)
                     /gene="sgtB"
                     /locus_tag="POB55_000721"
     CDS             complement(6318..7127)
                     /gene="sgtB"
                     /locus_tag="POB55_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional peptidoglycan glycosyltransferase
                     SgtB"
                     /protein_id="HDI6499706.1"
                     /translation="MKRSDRYSNSNEHFEHMKHEPHYNTYYQPVGKPPKKKKSKRILL
                     KILLTILIIIALFIGIMYFLSTRDNVDELRKIENKSSFVSADNMPEYVKGAFISMEDE
                     RFYNHHGFDLKGTTRALFSTISDRDVQGGSTITQQVVKNYFYDNDRSFTRKVKELFVA
                     HRVEKQYNKNEILSFYLNNIYFGDNQYTLEGAANHYFGTTVNKNSTTMSHITVLQSAI
                     LASKVNAPSVYNINNMSENFTQRVSTNLEKMKQQNYINETQYQQAMSQLNR"
     gene            complement(7466..7981)
                     /locus_tag="POB55_000722"
     CDS             complement(7466..7981)
                     /locus_tag="POB55_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HDI6499707.1"
                     /translation="MTKKVAIILANEFEDIEYSSPKEALENAGFNTVVIGDTANSEVV
                     GKHGEKVTVDVGIAEAKPEDYDALLIPGGFSPDHLRGDTEGRYGTFAKYFTKNDVPTF
                     AICHGPQILIDTDDLKGRTLTAVLNVRKDLSNAGAHVVDESVVVDNNIVTSRVPDDLD
                     DFNREIVKQLQ"
     gene            complement(8112..8273)
                     /locus_tag="POB55_000723"
     CDS             complement(8112..8273)
                     /locus_tag="POB55_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499708.1"
                     /translation="MNEQQTIEQIKARLNKFIEDIDHVNPDEVRVEDIDEWIGLLDQL
                     EEKVKLVSK"
     gene            8497..9648
                     /gene="yfkAB"
                     /locus_tag="POB55_000724"
     CDS             8497..9648
                     /gene="yfkAB"
                     /locus_tag="POB55_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/CxCxxxxC motif protein YfkAB"
                     /protein_id="HDI6499709.1"
                     /translation="MNSVKLKQPVSIYNDPWESYNDVKEHGQLTLSNIEFTTTNLCNM
                     RCSHCAVGYTLQTVDPEPLDMDLIYRRLDEIPNLRTMSITGGEPMFSKKSIRNVVKPL
                     LKYAHHRGIYTQMNSNLTLPQDRYLDIAEYIDVMHISHNWGTTDEFANVGFGAMKKQP
                     PLKAKLKLYEQMISNARTLSEQGMFVSAETMLNQSTLPHLRKIHQEVVHDMKCSRHEI
                     HPMYPADFASQLNVLTLAEMKKTIHDILDFRDEDIWMLFGTLPVFPCLKDDEDQKLLS
                     RLRTAKNVTTRNDPDGRSRLNVNVFTGNVIVTDFGDETGTISNIQKDKLTDVFDKWLS
                     SDLAKSLNCHCSEFSCLGPNVLVKNMYYPNMDFKDNERHMHKQPQIIQF"
     gene            complement(9908..10438)
                     /locus_tag="POB55_000725"
     CDS             complement(9908..10438)
                     /locus_tag="POB55_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HDI6499710.1"
                     /translation="MTNQDRPMKSMSESKCYKNRQVFPQDTNHHHTMFGGTLMANIDE
                     IAAITAMKHAGAQVVTASTDSVDFLKPIKTGDILQYVAMVSYAGTSSMEVVVQIRIDD
                     VFNNKHDLAALSYLTFVALDDEGKPKHVPGVYPEDDVEKWFYDTAPQRVERRKARRIE
                     SKQTIEYLAQVQHIRD"
     gene            complement(10527..11783)
                     /locus_tag="POB55_000726"
     CDS             complement(10527..11783)
                     /locus_tag="POB55_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDI6499711.1"
                     /translation="MGILTNYKEKLQQYAELLVKVGMNVQPKQPVFIRSSVETLELTH
                     LIVEEAYHCGASDVRVVYSDPTLKRLKFENESVEHFANHEIKSYDVEARMDYVKRGAA
                     NLALISEDPDLMDGIDSQKLQAFQQQNARAFKGYMESVQKNQFPWVVAAFPSKAWAKS
                     VYPELSVEEAYIKFIDEVFDIVRIDGNDPVENWRQHIANLSVYAQKLQQKNYHALHYV
                     SEGTDLTVGLAKNHIWEDATSYVNGKEQAFIANIPTEEVFTAPDRNRVDGYVTNKLPL
                     SYNGTIIDQFKLMFKDGEIIDFSAEKGEAVLKDLINTDEGSRRLGEVALVPDDSPISN
                     RNTIFYNTLFDENAACHLAIGSAYAFNIQGGTEMTVEEKIASGLNDSNVHVDFMIGSS
                     DLTIYGIFEDGSKELVFENGNWASTF"
     gene            complement(11786..11986)
                     /locus_tag="POB55_000727"
     CDS             complement(11786..11986)
                     /locus_tag="POB55_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1128 family protein"
                     /protein_id="HDI6499712.1"
                     /translation="MTNEEKVLAIREKLNIVNQGLLDPEKYKNANEEELTDIYDFVQS
                     RERLSPSEVTAIADALGQLRHD"
     gene            12133..12597
                     /locus_tag="POB55_000728"
     CDS             12133..12597
                     /locus_tag="POB55_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HDI6499713.1"
                     /translation="MVDVAFVCLGNICRSPMAEAIMRQRLKDRNIHDIKVHSRGTGSW
                     NLGEPPHEGTQKILNKHNIPFDGMISELFEATDDFDYIVAMDQSNVDNIKSINPNLKG
                     QLFKLLEFSNMEESDVPDPYYTNNFEGVYDMVLSSCDNLIDYIVKDANLKEG"
     gene            12604..12879
                     /locus_tag="POB55_000729"
     CDS             12604..12879
                     /locus_tag="POB55_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDI6499714.1"
                     /translation="MENKFVPGILIGAVIGGAISLADKSTRQALVQSVKDAKNGNRTR
                     KPSKVSKIKDEVLYWKDVVEEIRRNNPELERSLKDAKETFVNRKNQR"
     gene            13157..14368
                     /locus_tag="POB55_000730"
     CDS             13157..14368
                     /locus_tag="POB55_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HDI6499715.1"
                     /translation="MSKKDHSSSKYLNSVKEAQEESKNKSNPKIDVDRTYIEPQQFQS
                     KKPKKDDQVFFLSRLNKPAKYKKDSNFLSYLIYRIGKDDASGLAAQMTYHFVLAMFPM
                     LLFLLTLLPFFNIKQSQITNMLSNAPAETSTLIKSVIGDITQNSSGGLLSIGLILAIW
                     SASNGMTAIMNSFNVAYDVEDSRNGIVLKLLSVVFTVVMGVVFVVALALPTLGSVISH
                     FLFGPLGFDEQVKWIFNLIRIVLPIIIIFIVFIVLYSVAPNVKTKLKSVLPGAVFTSI
                     IWLAGSFGFGWYISNFGNYSKTYGSIAGIIILLLWLYITSFIIIVGAEINAIIHQRSV
                     IKGKTPEEAALEHDDNNKNHYNENTRYEYDEDSNATHQRTYHVDEHPSDTYPEDDKNI
                     KDKIVDKLKKD"
     gene            complement(14480..15109)
                     /gene="vraR"
                     /locus_tag="POB55_000731"
     CDS             complement(14480..15109)
                     /gene="vraR"
                     /locus_tag="POB55_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator VraR"
                     /protein_id="HDI6499716.1"
                     /translation="MTIKVLFVDDHEMVRIGISSYLSTQSDIEVVGEGASGKEAIAKA
                     HELKPDLILMDLLMEDMDGVEATTQIKKDLPQIKVLMLTSFIEDKEVYRALDAGVDSY
                     ILKTTSAKDIADAVRKTSRGESVFEPEVLVKMRNRMKKRAELYEMLTEREMEILLLIA
                     KGYSNQEIASASHITIKTVKTHVSNILSKLEVQDRTQAVIYAFQHNLIQ"
     gene            complement(15099..16142)
                     /locus_tag="POB55_000732"
     CDS             complement(15099..16142)
                     /locus_tag="POB55_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDI6499717.1"
                     /translation="MNHYIRTIGSMLILVYSMLAAFLFIDKVFVNIIYFQGMFYTQIF
                     GIPVFLFLNLIIILLCIIVGSVLAYKINQQNDWIKTQIERSMEGETVGINDQNIEIYS
                     ETLDLYHTLVPLNQELHKLRLKTQNLTNENYNINDVKVKKIIEDERQRLARELHDSVS
                     QQLFAASMMLSAIKETKLEPPLDQQIPILEKMVQDSQLEMRALLLHLRPLGLKDKSLG
                     EGIKDLVIDLQKKVPMKVVHEIQDFKVPKGIEDHLFRITQEAISNTLRHSNGTKVTVE
                     LFNKDDYLLLRIQDNGKGFNVDEKLEQSYGLKNMRERALEIGATFHIVSLPDSGTRIE
                     VKAPLNKEDSYDD"
     gene            complement(16139..16840)
                     /locus_tag="POB55_000733"
     CDS             complement(16139..16840)
                     /locus_tag="POB55_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HDI6499718.1"
                     /translation="MTHKYISTQMLIIFTALMIIANFYYIFFEKIGFLLVLLLGCVLV
                     YVGYLYFHKIRGLLAFWIGALLIAFTLLSNKYTIIILFVFLLLLIVRYLIHKFKPKKV
                     VATDEVMTSPSFIKQKWFGEQRTPVYVYKWEDVQIQHGIGDLHIDLTKAANIKENNTI
                     VVRHILGKVQVILPVNYNINLHVAAFYGSTYVNEKSYKVENNNIHIEEMMKPDNYTVN
                     IYVSTFIGDVEVIYR"
     gene            complement(16855..17241)
                     /locus_tag="POB55_000734"
     CDS             complement(16855..17241)
                     /locus_tag="POB55_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499719.1"
                     /translation="MNYVERYIEQFLRATVRNNIKHYLLMLDEKMKNLDDYMRYLITK
                     KEQLSKLIDSLMLTLENKYIDIAEAFQIQCAREINNQEIENIKSELNKVEAYYAQIET
                     QIQQTSTEKIATEKTSYLINYMNAVA"
     gene            complement(17458..18216)
                     /gene="map"
                     /locus_tag="POB55_000735"
     CDS             complement(17458..18216)
                     /gene="map"
                     /locus_tag="POB55_000735"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDI6499720.1"
                     /translation="MIVKTEEELQALKEIGYICAKVRNTMQAATKPGITTKELDNIAK
                     ELFEEYGAISAPIHDENFPGQTCISVNEEVAHGIPSKRVIREGDLVNIDVSALKNGYY
                     ADTGISFVVGESDDPMKQKVCDVATMAFENAIAKVKPGTKLSNIGKAVHNTARQNDLK
                     VIKNLTGHGVGLSLHEAPAHVLNYFDPKDKTLLTEGMVLAIEPFISSNASFVTEGKNE
                     WAFETSDKSFVAQIEHTVIVTKDGPILTTKIEEE"
     gene            18747..19733
                     /locus_tag="POB55_000736"
     CDS             18747..19733
                     /locus_tag="POB55_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HDI6499721.1"
                     /translation="MNDQWYKHLIGARTIKTGIAIFLTAVFCMALDLTPIYAILTAVV
                     TIEPTAKASLIKGYRRLPATVIGAGFAVLFTYLFGDQSPFTYALSATFTILFCTKLKL
                     QVGTNVAVLTSLAMIPGIHDAYIFNFLSRTLTAIIGLVTSGLINFMVFPPKYYGQVEE
                     KLSKTDALMYKLFYNRCQELILSRLQSDKSEKAYKNIFNLNNQVETLISYQRDELSYH
                     KKKECDWKLLNQLTKRAYTNRLFITHLSNIIYLPKNTRVNFSGDEKMALLKISSSIKD
                     IFYDGSFKREDDSVETLRSTIKALEISGENQIKSHILYEVLMIYRLLDSRYA"
     gene            complement(20164..20577)
                     /locus_tag="POB55_000737"
     CDS             complement(20164..20577)
                     /locus_tag="POB55_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499722.1"
                     /translation="MKPTKLILKDASYLHSKTSITFILKDVVIEEDNKIYYFDTSATF
                     EDQEVKFEFALFDSDMDNLKHMDYDNPVTEIYFIEPDLHFTIIDFNQELLCIYIDFDS
                     GLRHSNMATESGISLRINVAKSDFTKFINELASLH"
     gene            complement(20606..20717)
                     /locus_tag="POB55_000738"
                     /pseudo
     CDS             complement(20606..20717)
                     /locus_tag="POB55_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500597.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(20803..21534)
                     /locus_tag="POB55_000739"
     CDS             complement(20803..21534)
                     /locus_tag="POB55_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HDI6499723.1"
                     /translation="MHELTIYHFMSDKLNLYSDIGNIIALRQRAKKRNIKVNVVEINE
                     TEGITFDECDIFFIGGGSDREQALATKELSKIKTPLKEAIEDGMPGLTICGGYQFLGK
                     KYITPDGTELEGLGILDFYTESKTNRLTGDIVIESDTFGTIVGFENHGGRTYHDFGTL
                     GHVTFGYGNNDEDKKEGIHYKNLLGTYLHGPILPKNYEITDYLLEKACERKGIPFEPK
                     EIDNEAEIQAKQVLIDRANRQKKSR"
     gene            complement(21536..22849)
                     /gene="murT"
                     /locus_tag="POB55_000740"
     CDS             complement(21536..22849)
                     /gene="murT"
                     /locus_tag="POB55_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II isoglutaminyl synthase subunit MurT"
                     /protein_id="HDI6499724.1"
                     /translation="MRQWTAIHLAKLARKASRAVGKRGTDLPGQIARKVDTDILRKLA
                     EQVDDIVFISGTNGKTTTSNLIGHTLKANNIQIIHNNEGANMAAGITSAFIMQSTPKT
                     KIAVIEIDEGSIPRVLKEVTPSMMVFTNFFRDQMDRFGEIDIMVNNIAETISNKGIKL
                     LLNADDPFVSRLKIASDTIVYYGMKAHAHEFEQSTMNESRYCPNCGRLLQYDYIHYNQ
                     IGHYHCQCGFKREQAKYEISSFDVAPFLHLNINDEKYDMKIAGDFNAYNALAAYTVLR
                     ELGLNEQAIKNGFETYTSDNGRMQYFKKERKEAMINLAKNPAGMNASLSVGEQLEGEK
                     VYVISLNDNAADGRDTSWIYDADFEKLSKQQIEAIIVTGTRAEELQLRLKLAEVEVPI
                     IVERDIYKATAKTMDYKGFTVAIPNYTSLAPMLEQLNRSFEGGQS"
BASE COUNT         7379 a         4030 c         3244 g         8292 t
ORIGIN      
        1 ttaaagtata taaaataatc acttttatat atcaacactt taactttttg acattgttat
       61 tctatgagat ttaaagatat catttatact ttttaaaatt aatgtcacta tgttttccga
      121 taatattacc aatcatcgaa tgttacccat ttataaattg ataaatcttt gacataggta
      181 cagggaatgt atattgatct cgatcactta aatcaaacca aatcatgtca tctggtaatg
      241 tttcaatgtt aattgctcct gaaacggcgt atactttaat cttccatgtt aaatgagtaa
      301 attgatgctt caactcaaaa ataggtgttt ctactggttg aatgtcatga ccgatttttt
      361 cagtcatttt acgtctagca tgctcacttt caaacatagg aaattgccac ataccatgca
      421 ataatttttc tctacgcttt tgcaacagat attgaccttg attatttcta attaaaaaga
      481 cggattgctc aattactttt ttacttacat ttttagattt aacaggtaac ttttcaaatg
      541 tacctttatc aaatgcctca cagttttctt gaactggaca aaataagcat aatggatttt
      601 ttggtgtaca aattaatgcc cctaattcca tcatagcttg attaaacgtt ccagcttctg
      661 tagtaacata cggtaacaat tcttgttcgt acgatttcct cgtcgattgt aatttaatat
      721 ctcgatagtc atcattcaat ctagaccata ctcgaaaaac atttccgtct acagttgcta
      781 gtggtacatt atatgcaatg ctcattactg cagcttgtgt gtatgggcca acacctttta
      841 acgctttaaa ttgatcagga tctttgggaa ctaagccttc atatttatca taaacttctt
      901 taatcgccgt atgaaaattt cgagctctac tataatatcc taagccttcc caatacttta
      961 acacttcatc ttccgaagct tgactcaaaa cttccacagt tggaaatcgt tcaacaaaac
     1021 gatgataata gtcaataact gttttaactt gtgtctgttg taacatgacc tcacttaacc
     1081 aaatatagta cggattggtc gtttgtcgcc atggcatttc tctttgattt tcatcaaacc
     1141 agtgtatcaa attttcttta aaactagact gctgatacat ttataaaacc ctttcctcac
     1201 caaaattaat tgtctttact cataatgttt ttattgtaca ttaaaatcat ggttagtatg
     1261 taagttaatt tagttatttg cgaaattgga ttataatagt atatataata ttatgaaatg
     1321 agtgaactga tatggacact gcaacacata tcgcaattgg ggtgggcctt acagcacttg
     1381 caactcaaga tccagcaatg gcttctacgt ttggtgcaac agctacaacc cttatcgttg
     1441 gttcattaat tcctgatggg gatactgttc ttaaattaaa ggacaatgca acatatattt
     1501 cgcatcatag aggtatcacg cattccatcc ctttcacaat actatggcca attttaatta
     1561 catttttaat attcacgttc tttagtggaa ccaacccatt tcatgtatgg atgtgggctc
     1621 agctcgcagt atttttacat gtctttgtag atatattcaa ttcttatggt acacaagcgc
     1681 ttagacctat cacaaacaaa tggattcaat taagtgtgat taacacattt gaccctatta
     1741 ttttcacagt tctttgtatt ggtattgtat tatgggttat aggcttgcat ccatttgcag
     1801 tcttctttcc tataatcgct ttactaatca tttattacat gattcgtttt aaaatgagag
     1861 ccgtaattaa gcaacaagct ttaaaagcaa ttcaacaaga gcatcaccct gttaaagtat
     1921 ttgttgcgcc aacaataaaa tttatggaat ggcgtgtcgc gatacaaact gatgcacatg
     1981 actatgttgg aaaagcatat ggtaggaatg tggtgtttag tgataaagtg gaacgtcaaa
     2041 cattatcaac agactccatt ttatggaaag tcaaaggtaa taaagatata cgtacttttt
     2101 taaacttttc atcaatctat cgttggcaaa caacaacgtt agcagatggt tctactgaaa
     2161 ttcgtttgat tgatttgcgt tatttaaaaa atgatcatta ttcatttgtg gcaattgcac
     2221 atgtaacaaa cgataatgtc atagaccact cttatattgg ctgggtattt acagaagata
     2281 agttacaacg taaactgtat gctaaataat ttcaagttat tattcactaa agttaatcta
     2341 taaaaaatga acaaccgggc agaatgaaaa tcaaaacgat ttttactctg tccggttttt
     2401 taatgtaaaa ctatgaatgc ttttacaaaa tctaaaattt atattgttgc taacaaacta
     2461 cctttaatga ctcgaaatat caaaatcagt ataggaaaac aatatctaga tgatattcta
     2521 attgtttctg attctcacag attaatttac acaacaggtc agctaaacat catgaagaag
     2581 tatccgcctc gtctgtacta tcatttgaaa catcctgttg attatcagtt tgtgtcactt
     2641 tagatgtttt attataaatt gcgtgtgtcg tataccttgc taatacgaaa tttactgcag
     2701 ctattaaaca taagaataaa ataaagtaaa aatgatatgg aatgttttcc atacttgata
     2761 taccaaatat gatagattgt gctataccat taacaatgta atacattgga tttagcatta
     2821 ggatgtgatt gataaataca tgatttggat ttggtatgaa aataattggt aacaataaga
     2881 aacacaatac acaaacccca taaaatatga tatttatttt ttcagttaac agtcgaataa
     2941 gaccaaaagt aacggatatt aatcctacaa aaatagttgc catcacaata aaatagaata
     3001 gcgctatata tgatgtttcg aagtttactg gtttaaccaa tgcactaatc atcgtcaaaa
     3061 tgactagcat aataaaactt aaaatagaca taataactac tggcgtcgct gcggaaatat
     3121 taaataactt agtaactaaa aagtcattca tataatgatg ataagagata taaattgtga
     3181 acatcactac cgcaaaaaca atcaatacaa tgattctaaa taaccatctc acctctttaa
     3241 catcggtcaa atgactaaag gaaaatagcg tttcacttgc aataataagt attaaacatg
     3301 cagctaatac gattagaaaa cttttccaac tttgttttaa acgttctgtt acataccttt
     3361 tagtatgcgg taagttttta aaatataaaa taatattatc tagcatcttt acacctacaa
     3421 ttcaatataa atccatttgt cttctttcaa attggcaata caatatccat tcccaacttt
     3481 agtaatccaa atgttattgt taatattaaa cttatctttt aattcttttt tatctacaat
     3541 tggataaaca aaaccgtata actgattatt atcattgaaa agtaattgta tcggttgttg
     3601 tgtaacggat gcaacgaaac ggttatcatc atcaggaact aaagttttta taactgaact
     3661 atgcttttta tgtaattgac tattaaagta tgcatagtaa ttactataat ttgatttcat
     3721 atatggtgct aattttttaa aactatgcgt ttgatataaa cctgccgggt taaaatattc
     3781 taatttatct ttactaacat agtaagtctt attatcggcg gtaacacgat aatttttact
     3841 attttcgcca gtaattgtaa ctacactata ttttggaatt gtgacttgtt tatccccttg
     3901 catatccaca ctgccattaa aagcaattcc ataagctaac ttttcgccat atggatcttt
     3961 attttcattt tgcatcgtag cacgattcgt aaaatctgtt atcgaaataa ttcctatatt
     4021 attgaaaatg agcaacatca tcaatgctaa gcctaaaata gtacctgagg ctaaagtcca
     4081 aaaacgcact aaaaacttag gcggttttgc atgattatag cgctcaacac gtttgaaatt
     4141 ataggtcatc tctggtattc ttgtacgatt ctttttccaa tctaaatcaa agttttcaat
     4201 ttcttctttt gagtttagac ttaatcgatc acgttcatgt tctttaaaag atggaataac
     4261 ttgtttaagt gacccttcca ttcttaattg accatgtgaa aaccaagcta tgtagttact
     4321 cacttgtgaa attttatcaa tatcatcacc aattgacaca atcgttaaat tattttcaat
     4381 ataatcattt gttaattcaa tcgcacgttc cataaattgt ggtgttaaat agtcaataac
     4441 atgatttaaa ataataatat ttgattttga tgagcgtgca atacttaata gtaattgagc
     4501 gtatgccgct ttcgaaatat ggttaaccgg cttcgtttta taatcaccta aatgtgcata
     4561 ttgaataatc tgttcagctt tatgatcatt aatttcatat ggaaatagtt gaactaactg
     4621 cgctgtataa gcttcaacag tttgatgaat taacgattga tcttcaatat atccgtagaa
     4681 caaatcttca gtacaaacta ctttaccttt atcaggttta attgcacctg ccaacaattg
     4741 acctaccaac gctttggaag attcaggttc accaattata cctaatgctt ctccttgata
     4801 aatatgtaaa ctaatattgt ttaaatcgat atcttcagca tcatatccaa aaggtaaata
     4861 ccatttctta ttctgtttat tcctatagta gtgtgttact tttagtaact ttaaaacaat
     4921 tgaacttccc atctattttc atccttctat aattgtaata acgctatagg taatccttct
     4981 tcgggttcat cgctattaaa tctaaacccc cacgcaaaaa taccttttaa tctttctact
     5041 ttaaaatatt gattactgcc atcagtcagt ttataaattt taccaatttc tatctttttt
     5101 ctgtctacta agtaactttc ggcaacaatt actttacttt gataaacgtc atattcattt
     5161 aaaataccat tcatctcagc ttttctcatt ttctctttat aaagttgtat ttcgtgtctt
     5221 aattctggct cagacatctc actcaatttc ttctgttcca tcggcaatac cactttcttc
     5281 taatttagct ttaattttat catatttata tccttttctc ataaggcctt cgatagtttt
     5341 tgaaattaat ttctgttgcg tgtacttctt tcgattttta ttataaattt tttctaaatc
     5401 tcgttgtaat aaatcgtcta aaaccgcttc atcttgtgta aaatccattt cattcaatac
     5461 agcatgaatg gtttccattt caaacccttt ttgaattaaa gattgcatta ctttttcttt
     5521 aactttattt tgtggccctt tttttgtttt agatattttt tcagcaattt ggataatatc
     5581 atcaagttcc tgttgttctc tataaagttc tgtaaatatt tcaatgatat ttggttctat
     5641 accaagttga tatagttttt gttgataaat tttaggtcct ttatctgtcg tgcgaatcat
     5701 tgtatttttt aaactttccg cataatcttg atggtcgatt aacttttcgc gataacaata
     5761 ttcaatcact tcagaaatcg cttgctctga tatctcttct ttttgtaaat attgtataac
     5821 ttctttttca gttctctttt tatatgataa atattggatt gctttgttta aacctatgcg
     5881 ataatgatca tacttttgaa tctctgccat gtcagcagct tcaagttgtt gccctttttt
     5941 taaattaaat ttgactaatg tatcgatatc tatacccatt tcaaattgtt cgtctaaaaa
     6001 aagattaaaa cgttctttgt ttttcttttg aacttctatt ttagtaatct tcggcactgg
     6061 atcaccccgt tactttctca agttttaata caatactctt ttattattca ataagccact
     6121 tcctatagca aatgtttaac tttaaatatt tttcgatgct aacaaaaaat cacactatca
     6181 tcttttaaaa tgaaagtgtg attacaagca aatctgtaaa atttataaag cagaaacaat
     6241 tcaactttat cattatgaca tttcaattaa accttctaca ttatagttcc aagcatctta
     6301 cacatgaatg caagtattta acgatttaat tgtgacatag cctgttgata ttgtgtttca
     6361 ttgatataat tttgttgctt cattttttct aagttcgtgc ttacacgttg cgtaaaattc
     6421 tctgacatat tattgatatt atatacgcta ggtgcattga ctttactagc taaaatagcg
     6481 ctttgtaaaa ctgttatgtg agacattgtt gtactatttt tattcacggt tgttccaaag
     6541 taatggtttg ctgcgccctc aagcgtatat tgattatccc caaagtaaat attatttaaa
     6601 taaaagctta aaatttcgtt cttattatat tgtttttcaa ctcgatgagc tacaaataat
     6661 tcttttactt ttctagtaaa tgaacgatca ttatcataaa aataattttt gacaacttgt
     6721 tgtgtaatgg tactaccacc ttgcacatct ctgtcgctaa tcgttgaaaa taaagctcta
     6781 gttgtacctt tcaaatcgaa tccatgatga ttgtagaatc gttcatcttc cattgaaata
     6841 aaggcacctt taacatactc tggcatgtta tcagctgaca caaaactact tttattttca
     6901 atttttctta gttcatccac attatcgcgt gtagataaaa aatacatgat accaataaac
     6961 aatgcgataa tgattagaat ggttaataat atttttaata gtattcgttt actttttttc
     7021 tttttcggcg gtttgccaac tggttgataa tacgtattat agtgaggttc gtgtttcata
     7081 tgctcaaaat gttcatttga gtttgagtac ctatcgcttc ttttcatgcg tttgctcctt
     7141 cttttaaaac tcacttagta tataccttga gtttaccagt actatcacaa ataggctaca
     7201 ctttttggga aaatcagtcc aagggcttac aatcgtatac gccatcatac ttactttttg
     7261 ttttttgaaa agattataga taaatcattg cgattttaaa tattaatcat gtcaaatatt
     7321 gttatatttt ataaaaataa aagaccatcc ctattaaatg ccaatagaga cgacctttta
     7381 tttgttattc attttttaaa actaaaatcc atatttcatt tcaaacgaaa atatataaat
     7441 tttaacaatt gataaccgca atactctatt gtaattgctt aacgatttct cgattaaaat
     7501 catctaaatc gtctggtact cgacttgtta caatattgtt gtctacaact actgactcat
     7561 caactacatg tgcgcctgca tttgataaat ctttgcgtac atttaatact gctgttaacg
     7621 tacgaccttt taaatcgtct gtatctatta gtatttgtgg cccatgacaa atggcaaatg
     7681 ttggtacatc atttttagta aagtatttag caaatgtgcc atatcgacct tctgtatctc
     7741 cacgtaaatg atctggtgaa aatcctccag gaattaataa tgcatcataa tcttctggtt
     7801 tagcttctgc aatgcctaca tcgacagtaa ctttttcacc gtgtttacca acaacttcac
     7861 tatttgcagt atctccaatc actacagtat taaagcctgc attctctaat gcctctttag
     7921 ggcttgaata ttctatatct tcaaattcgt ttgctagaat aattgctact tttttagtca
     7981 ttgaaaatca cctttctata tatcattgat ataattacta tagacaagta aatcagtgat
     8041 taaacataca agatataaaa attattaagc gactgtcgcg atatctaacc ctaacacatc
     8101 ttatgtggca tttacttaga tactaattta accttttctt caagctgatc taacaatcca
     8161 atccattcat ctatatcttc aacacgtact tcatcaggat ttacatgatc gatatcctca
     8221 ataaacttat ttaaacgcgc ttttatctgt tcgattgttt gctgttcatt cataaaaagt
     8281 taactccttt tattttgttt tctttttcat tattatccta acagaaattg cgttaaagcg
     8341 atataatctt agctatattt atgacattca aattattttg acttttaaaa atcccctttt
     8401 caattaacta aaattaagag ataatttgtt acgagtgata atacgaagtg gtatcatacc
     8461 gatatgaacc aaatagaaag aaggaagttt aagacgatga atagcgtcaa attgaagcaa
     8521 cctgttagca tttacaatga tccatgggaa tcatataacg atgttaaaga acatggccaa
     8581 ttaactttaa gtaacatcga atttacaact acaaatcttt gtaatatgcg ttgtagccac
     8641 tgtgcagttg gttatacttt acaaactgtc gaccccgagc ctttagatat ggacttaatt
     8701 tatcgtagac ttgatgaaat tccaaatctg cgaacgatgt caattacagg tggcgaacca
     8761 atgttttcta aaaagtctat tagaaatgtt gttaaacctc tattaaagta tgcacatcat
     8821 cgaggtatat atacacaaat gaattcaaac ctaacattgc ctcaagatcg ttatttagat
     8881 attgctgaat atatcgatgt tatgcatatc tcacataact ggggaacaac tgatgaattc
     8941 gcaaatgttg gctttggcgc aatgaagaag caaccaccgt taaaagctaa gttaaaatta
     9001 tatgaacaaa tgatttcgaa tgcacgtaca ttatcagaac aaggaatgtt tgtatctgcg
     9061 gaaacaatgc tcaatcaaag tacgctacca catttacgaa aaatacatca agaagtcgtt
     9121 catgatatga aatgtagcag acacgagatt caccctatgt atccagctga ctttgcaagt
     9181 caattaaatg tgttaactct agcggaaatg aaaaagacaa ttcatgatat attggatttc
     9241 agagatgaag atatttggat gttatttggt actttgcctg tgtttccatg cttaaaagat
     9301 gatgaagatc aaaagttact atcacgttta agaactgcta aaaatgtaac gactagaaat
     9361 gacccggatg gccgtagtcg tttaaatgtc aatgtattta caggtaatgt aatcgtaact
     9421 gatttcggag atgaaacagg tacaatttcg aatatacaaa aagataaatt aacagatgta
     9481 tttgataaat ggttatcctc tgatcttgct aaatcattaa attgtcattg ttccgagttt
     9541 agttgtttag gaccaaatgt tcttgttaaa aatatgtact atccgaatat ggattttaaa
     9601 gataatgagc gtcatatgca caaacaacca caaattatac aattttaaaa actcttaatt
     9661 atgcggagaa gcactttatc gataagtagt ctccgcatat tttaatgcta ttataaaata
     9721 aaaaacaatt aattgctggc agtactctac tttaataata aaggtcattt aataggacta
     9781 atagtctata ataaaagggt taaattttaa ctaaaagcat aaacgtgcat aatcaaaaag
     9841 acagattgta ggtggaatat tcgaacataa cagttcaatt catccttaac aatctgtctt
     9901 tatattttta gtctctaata tgttgcactt gagctaaata ttcaattgtt tgtttacttt
     9961 caatgcgacg tgcttttctt cgttcaacac gttgtggtgc tgtatcataa aaccattttt
    10021 caacatcatc ttctggatat acaccaggta catgtttagg tttgccttca tcatctaacg
    10081 caacaaatgt taggtaactt aatgcagcta aatcatgctt gttattaaat acatcgtcaa
    10141 ttctaatttg aacgaccact tccattgaac tagtcccagc gtatgaaacc atcgctacgt
    10201 attgtaatat gtcccctgtt ttaatcggct ttaagaaatc tactgagtct gtagatgcgg
    10261 taactacttg cgcaccagca tgtttcatag ctgtgattgc tgcaatttca tcaatattag
    10321 ccatcaatgt accaccaaac attgtatgat ggtgattcgt atcttgaggg aaaacttgtc
    10381 tatttttata acattttgat tctgacatag atttcattgg tctgtcctga tttgtcatta
    10441 ttttatctcc caatctatac tttaatcacg tatttgacct ttaaatacat gtttcatttc
    10501 ctactcagca cctcaaaata catttattaa aatgttgatg cccaatttcc attttcaaat
    10561 actagttctt ttgaaccatc ttcgaatatg ccataaatag tcaaatcact actaccaatc
    10621 atgaaatcga catgtacgtt tgaatcattt aatccgcttg caatcttttc ctcaacagtc
    10681 atttccgttc caccttgaat attaaaggcg taagcagatc caatcgctaa atgacatgct
    10741 gcattttcat caaataaagt attgtaaaaa atggtattac gatttgatat cggtgaatca
    10801 tcaggtacta atgctacttc acctaatctt cttgaacctt catcagtatt gattaaatct
    10861 ttcaataccg cttcaccttt ttcagctgaa aaatcaataa tctctccatc tttaaacatc
    10921 aatttaaatt gatcgataat tgtgccatta taacttaaag gcaatttatt agtaacatat
    10981 ccatctactc tatttcgatc tggtgctgta aatacttctt cagttggtat attagcaata
    11041 aaagcttgtt ccttgccgtt aacataactt gtagcatctt cccagatatg atttttcgct
    11101 aaacctactg ttaaatcagt accttcagat acataatgca acgcatggta atttttttgt
    11161 tgtaactttt gagcgtaaac acttaaatta gcgatgtgtt gacgccaatt ttcaactgga
    11221 tcgttaccat caattcgcac aatatcgaat acctcgtcaa taaacttaat atatgcttct
    11281 tccacagata gttcaggata cacactcttc gcccaagctt tagatggaaa agcagctacg
    11341 acccatggaa attgattttt ttgtacactt tccatatatc ctttaaatgc gcgtgcgttt
    11401 tgttgttgaa atgcttgtaa tttttggcta tcgataccat ccattaaatc aggatcttca
    11461 ctaataagtg ctaaattagc agcgccacgt ttaacgtaat ccattctcgc ttcaacatca
    11521 tatgatttta tctcatgatt agcaaaatgt tccacagatt cattttcaaa ttttaagcgt
    11581 tttaaggttg gatcgctata tacaacacgt acatctgaag cgccacaatg atatgcttct
    11641 tctacaatta aatgcgttaa ttctaatgtt tctactgatg atctaataaa tactggctgt
    11701 tttggttgaa cattcatacc aaccttaact aatagttcag cgtattgttg taacttttct
    11761 ttgtaattag tcaaaattcc cactcctagt cgtgtcgcaa ttgtcctaaa gcgtcagcaa
    11821 tagctgtcac ttcacttggc gacaatcttt ctcttgattg aacaaaatca tatatatctg
    11881 ttaattcttc ttcatttgca tttttatatt tttcaggatc taataatcct tgattaacaa
    11941 tatttaactt ctctctaata gctaatactt tctcttcatt tgtcatagcc acaatgttca
    12001 cctctaataa tagttatgtt atcaaagtat cacaatttaa taaactttaa tagaatttta
    12061 tataatctgc tttcttattg tttaaagtcg ctataaaatg gggatataac tacatagttt
    12121 ggaggaatca aaatggtaga tgtagcattt gtctgtcttg gcaatatatg tcgttctcca
    12181 atggcagaag caatcatgcg acaaagactt aaagacagaa atattcatga tattaaagta
    12241 cattcaagag gtactggtag ctggaattta ggagagccac ctcatgaagg tacacaaaaa
    12301 attctcaaca aacacaatat tccatttgat ggcatgatta gtgaattatt cgaagcgaca
    12361 gatgattttg attacattgt ggctatggat caaagtaacg ttgataatat taaatctatc
    12421 aatcctaatc ttaagggaca attgttcaaa ctgttagaat ttagtaatat ggaagagagt
    12481 gatgtaccag atccatacta cacgaataat tttgaaggtg tatacgacat ggtattatca
    12541 tcttgtgata atttaataga ctacatcgta aaagatgcaa atttgaaaga ggggtagttt
    12601 tttatggaaa ataaatttgt tcctggtatt ttaattggtg ccgtaattgg tggtgcaatt
    12661 agtttagctg ataaatctac acgtcaagct ttagttcaat cagttaaaga tgcaaaaaat
    12721 ggtaaccgca ctcgtaagcc ttctaaagtc agcaagatta aagacgaagt tttatactgg
    12781 aaagatgttg ttgaagaaat tcgtcgtaat aatcctgaat tagaacgttc attaaaagat
    12841 gcgaaagaaa catttgttaa tagaaaaaat caacgctaag atgattttgt ttttataagg
    12901 caatacttaa caatttggtg tacgcctttc atagttatca ttattaaatg ataatacaat
    12961 gattggccaa actgcattgt ttcagtattg cctttgtttt aattttaaat caaaatagcc
    13021 tatgaaagat ttaaatcaat taatttctat aatattatca tttttaaagc atatcattgt
    13081 ttagtttttt tataattgga taaatactaa tagttacttt ataaaacatt acatagagaa
    13141 aggttaagga gtgcacatgt cgaaaaagga tcactcttct tcaaaatatc ttaattctgt
    13201 taaggaagca caagaggagt caaaaaataa aagtaatccc aaaattgatg ttgatcgtac
    13261 atatattgaa cctcaacaat tccaatctaa gaaacctaaa aaagatgatc aggttttctt
    13321 cttatcaaga ttaaataaac ctgcaaaata taagaaagac tctaatttct tatcatatct
    13381 catctatcgc ataggaaaag atgatgcctc aggactagca gcacaaatga cttaccattt
    13441 cgtacttgct atgttcccta tgttgctttt cctattaaca ttattaccat ttttcaatat
    13501 taagcagagt caaattacta atatgttaag caatgcaccc gctgaaacat ctactctaat
    13561 taagagtgta attggtgata taactcaaaa ctccagtggt ggcttattat ctatcggttt
    13621 gattttagca atttggtcag cttcaaatgg aatgactgca attatgaatt ctttcaatgt
    13681 tgcttacgat gtagaagata gccgtaatgg aatcgtatta aaactactaa gtgttgtctt
    13741 cactgtagtt atgggcgttg tgtttgtagt tgctctagca ttaccaacgc ttggttctgt
    13801 aattagtcat ttcctattcg gtccacttgg atttgacgaa caagtgaaat ggatttttaa
    13861 ccttattaga attgtgttac caatcattat tatatttatc gtatttatcg tgttatattc
    13921 ggttgcacct aacgttaaaa cgaagcttaa gtcagtatta ccaggtgcag tatttacttc
    13981 aattatttgg ttagctggtt catttggttt tggttggtat atttcaaatt ttggtaacta
    14041 ttctaaaaca tatggcagta tcgcgggtat catcattttg ttactatggt tatatatcac
    14101 aagttttatt ataattgtcg gtgctgaaat caatgcaatc attcatcagc gtagtgtaat
    14161 taaaggtaaa acacctgaag aagcagcatt agaacatgat gacaataata aaaatcatta
    14221 taatgagaac actagatacg aatatgatga agatagtaat gcaacacatc aacgtacgta
    14281 ccatgttgat gaacatccta gtgataccta tccagaagat gataaaaata taaaagacaa
    14341 aatcgttgat aagcttaaaa aagactaaac accaacaaaa cagaggtttt cgctaatgat
    14401 tgcgaagacc tctgtttttg gtgtctattt gatgttatat tgtgattggc gtaagtaact
    14461 tttcttaatt caatacgaac tattgaatta aattatgttg gaatgcatag atgacagctt
    14521 gtgttctatc ttgcacttct aacttactta aaatgttact cacatgtgtc ttaaccgttt
    14581 taatagtaat atgcgatgca ctagcaattt cttgatttga gtaacctttc gcaatcaata
    14641 ataatatttc catttctcgt tctgtaagca tttcatataa ctctgcgcgc tttttcatac
    14701 ggttacgcat tttcactaaa acttccggtt caaaaacaga ttctcctcta gaagttttac
    14761 gaactgcatc ggcgatatct tttgcacttg ttgtttttaa aatgtaacta tcgacacctg
    14821 catctaatgc acgatatacc tctttatctt caataaaact agttaacatt aatactttaa
    14881 tttgcggtaa atctttttta atctgagtcg tcgcttctac accatccatg tcttccataa
    14941 gtaaatccat taaaattaaa tctggcttca actcatgggc tttggcaatt gcttccttac
    15001 cagaagcgcc ttcaccaact acttcaatat cactttgcgt tgatagataa cttgaaattc
    15061 ctatacgtac catttcatga tcatccacaa acaatacttt aatcgtcata cgaatcctcc
    15121 ttatttaaag gtgctttcac ctcgatacgt gtacctgaat ctggcaatga tacaatatgg
    15181 aacgttgcac caatttccaa agctctttca cgcatatttt taagtccata actttgttct
    15241 aatttttcat caacattaaa acctttacca ttgtcttgaa ttctcaacaa taaataatcg
    15301 tctttattaa acaattctac tgtcactttt gtaccgtttg aatgacgcaa tgtattcgaa
    15361 attgcttcct gtgtaattct gaacaaatga tcttcaatac ctttaggcac tttaaaatct
    15421 tgtatttcat gcacaacttt catcggcact tttttttgta aatcaataac taaatcttta
    15481 ataccctcac ctaaagattt gtcttttaaa ccaagcggtc ttaaatgtaa cagcaaagca
    15541 cgcatttcta actgcgaatc ttgaaccatt ttctctaaaa tcggaatttg ttggtctaat
    15601 ggtggttcta acttcgtttc tttgatagca gaaagcatca tacttgccgc aaaaagttgc
    15661 tgactaacag aatcgtgaag ttctcgagct agtctttgac gttcatcttc aataatcttt
    15721 ttaactttca catcattaat attataattt tcattggtta aattttgagt tttaagtcgc
    15781 aacttatgca attcttgatt taaaggtacg agtgtatggt ataaatctaa cgtttcacta
    15841 tatatttcta tattttgatc attaatgcca actgtttcgc cttccattga acgctcaatt
    15901 tgcgtcttaa tccaatcatt ttgctgattg attttgtatg cgagtaccga accaacaata
    15961 atacacaata atatgatgat gagatttaaa aataaaaaga ctggtattcc gaatatttgt
    16021 gtataaaaca taccttgaaa atagatgata tttacaaaaa ctttatcgat gaatagaaat
    16081 gcagctagca tgctatatac taagatgagc attgaaccaa ttgttctaat gtagtggttc
    16141 atcgataaat cacctctacg tctccgataa acgttgatac gtagatatta actgtatagt
    16201 tatccggttt catcatttct tcaatatgaa tattgttatt ttcaacttta tatgattttt
    16261 cattcacgta agtacttcca taaaaagcag ctacatgtaa attaatattg taattaaccg
    16321 gcaatataac ctgcacttta cctaaaatgt gtctaacaac aatggtatta ttttccttaa
    16381 tatttgcagc ttttgttaag tcaatatgta ggtcgccaat tccatgttga atttggacat
    16441 cttcccattt atatacataa actggtgtac gttgctcacc aaaccacttt tgtttaataa
    16501 aagatggtga agtcataacc tcatccgtcg caactacttt ttttggttta aatttgtgta
    16561 ttaaataacg cacaataagt agtaataaaa agacgaacaa gatgatgatt gtatacttat
    16621 tagacaataa tgtgaatgca attaatagcg cgcctatcca aaacgccaaa aggccacgta
    16681 ttttatgaaa ataaagatat cctacataaa ctaatacaca tcccaataat agaacgagta
    16741 aaaagccaat tttttcaaaa aatatgtagt aaaaattggc aataatcatt aatgcagtaa
    16801 aaatgatcaa catttgcgtt gatatatatt tgtgtgtcat gtttcgccgc ctttctatgc
    16861 cacagcgttc atataattta ttagatacga tgttttttct gttgctattt tttcagttga
    16921 agtttgttga atttgagttt caatttgtgc ataatatgct tcaactttat tcaactctga
    16981 tttaatattt tcaatttctt gattattgat ttctcttgca cattgaattt gaaatgcttc
    17041 agcaatatca atatatttat tttctaatgt tagcattaga ctgtcaatta acttgctaag
    17101 ttgttctttt ttagtaatta aataacgcat ataatcatct aaatttttca ttttttcatc
    17161 tagcattaaa aggtagtgct tgatattatt tcttactgtt gctctcaaaa actgttcaat
    17221 ataacgttca acatagttca taactatcac cttttataat aagttttaaa ataccaaatg
    17281 cgctatgtat cttcgttgat gtcgatgata tgtttgctta aaaactactt tagtacaaca
    17341 tatttttgaa atgacgcatt gattgtgttc ttaattatta taaaacagtt ccgaaaccaa
    17401 tatgaatagg ctccggaact aaatgatgtt catactttag tcttagtata tgttgaacta
    17461 ttcttcttca atttttgtcg ttaaaatcgg accatcctta gtcacgataa ccgtatgctc
    17521 aatttgagca acaaaacttt tatcgctcgt ttcaaaagcc cattcatttt taccttctgt
    17581 aacaaatgat gcatttgatg agataaacgg ttcaatagct aataccatac cttcagttaa
    17641 taatgttttg tcttttggat caaagtaatt aagtacatgt gctggtgctt catgtaatga
    17701 taaaccaaca ccatgacctg ttaagttttt aatgactttc aaatcatttt gtctagctgt
    17761 attatgcacc gctttaccaa tgttacttaa cttagtaccc ggttttactt ttgcaattgc
    17821 attctcaaat gccatcgttg ctacgtcaca tactttttgt ttcattggat catctgattc
    17881 tccaacgaca aatgaaatgc ctgtatctgc ataatagcca ttcttcaaag ccgatacatc
    17941 aatatttact aaatctcctt cacgaatgac acgcttactt ggaatcccat gtgccacctc
    18001 ttcattgaca ctaatacacg tttgaccagg aaaattttca tcatgaattg gcgcagaaat
    18061 agcaccgtat tcttcaaata actctttcgc aatattatca agctctttcg tagtgatacc
    18121 tggtttggtt gcagcttgca ttgtattgcg cactttagcg catatgtatc caatttcttt
    18181 taacgcttgt aattcttctt ctgtttttac aatcatttta tcccgttcct tttttatata
    18241 attataactt attatagcaa aaattctttg atatactaaa gatgttactt tagtattttg
    18301 gaaagtgagt gagacatagt gtgataaaga aaaaatattt ctttatcgtt gtaccccaac
    18361 ttgcattgcc tgtagaaatt gtagatccaa tttctctatg ttggggcccc gccaacctgc
    18421 acattatcgt aagctgactt ttcgtcagct tctgtgttgg ggcccacacc ccaacttgca
    18481 ttgcctgtag aatttctttt ttgaaattct ctatgttggg gccccggact ataattgaaa
    18541 aatgcttgtt acaagtgcat tttatttcag tcaactacta acaatataac attgtggagc
    18601 ccagaacttt gattagtgta tatgaaaatc aaagtaatgc ttaagtatga ttatttcaaa
    18661 tattttacat acatgaactt ttccaatgta cgatactatt attataaagc gctcgctaag
    18721 attttacgat gattagaggt taaaaaatga acgatcaatg gtataaacat ttaattggtg
    18781 ccagaacaat taaaactggt attgccattt ttttaacagc tgtcttttgt atggcactag
    18841 atttaacacc catctatgcc attttaacag ctgtagtcac aattgaacca actgccaagg
    18901 catcacttat taaaggttat cgtagattac ctgctacggt aattggtgca ggctttgcag
    18961 tattatttac atatttattc ggcgatcaat caccttttac atatgcattg agtgcaacgt
    19021 tcacgatttt attctgtaca aaactcaaat tgcaagttgg tacgaatgtc gctgtactaa
    19081 catcattagc tatgattcca ggtattcatg atgcctacat attcaacttt ttgtctcgaa
    19141 cattgacagc aattattgga ctcgtgacat caggtttaat taacttcatg gtgtttccac
    19201 ctaagtatta tggtcaggtc gaagaaaaat taagtaagac agatgccttg atgtataaat
    19261 tattttacaa tcgctgccaa gaacttatct tatcaagact gcaatctgat aaaagcgaaa
    19321 aagcatataa aaatattttc aatttaaaca atcaagttga aacgttaata agttatcaac
    19381 gtgatgaatt aagctatcat aagaaaaaag aatgtgattg gaaattactc aatcaattaa
    19441 ctaaacgtgc atatacgaat cgtttgttta tcactcattt atcaaatatc atttacttac
    19501 ctaaaaatac tcgtgttaac ttttcaggtg acgaaaaaat ggcattgcta aaaattagta
    19561 gtagcatcaa agatatattt tatgatggtt cttttaagag agaagatgat tctgtagaaa
    19621 ctttacgttc gacaataaaa gcacttgaaa ttagtggtga aaatcaaatt aaaagtcata
    19681 tactatacga agttctaatg atttatcgac tgctagacag cagatatgct taatttaagt
    19741 accttatact aattggcacc atgggagtgg gacagaaatg atattttcgt aaaatttatt
    19801 tcgtcgtccc accccaactc gcattgcctg tagaatttct tttcgaaatt ctctgtgttg
    19861 gggcccacac cccaacttgc attgtctgta gaaattggga atccaatttc tctttgttgg
    19921 ggcccctgac tagaattgaa aaaagcttgt tacaagcgca ttttcgttca gtcaactact
    19981 gccaatataa cttcgtagag catagaacat tgatttatgt ccccgcctct ttttatcgta
    20041 tatagcatgt ccctttttaa aattacaagc ataaatattc agactcaaat atagccacac
    20101 ccctaaacta cggagggatg tggctgtctt ttttgattgt aattatgtat cagatttaaa
    20161 tcattaatgt aaagaggcta attcattaat aaatttagta aaatctgatt tagcaacatt
    20221 tatccttaat gaaataccag attctgttgc catgtttgaa tgccttaaac cagaatcaaa
    20281 atcaatataa atacaaagca gttcttgatt aaaatcaata attgtaaaat gtaaatcagg
    20341 ttctataaaa tatatttcag taacaggatt gtcgtaatcc atatgtttca aattgtccat
    20401 gtcactatca aaaagtgcaa attcaaattt aacttcttga tcttcgaaag ttgcactagt
    20461 gtcgaaataa taaattttat tatcttcttc gataactaca tcttttaaaa taaatgttat
    20521 cgatgttttg ctatgtaaat aagatgcatc ttttaatatc aatttagtag gcttcatcgt
    20581 caaattcctt tcatcattca ttttttcatg ttgcataatc accattacta ttgataattc
    20641 taggatagat actcattgta taataattat tcactagaac aaaaaaaaca cacatcaatt
    20701 tggttgtttc catccaaaat aatgtgtgtg tttatacata tatgattaat tactaaactt
    20761 catcgtttat caatattaaa tccattgatg cgatgttcag agttaacgag atttcttctg
    20821 tctatttgct ctgtctatta atacttgttt cgcttgtatt tccgcttcat tatctatttc
    20881 tttaggctca aacggaatac ccttacgttc acaagctttt tctaacagat aatcagtgat
    20941 ttcgtaattt ttaggtaaaa ttggtccgtg taaataagta cctaataaat ttttataatg
    21001 aatgccttct tttttatctt catcattatt accataacca aaagtgacat gaccaagtgt
    21061 accgaaatca tgatatgttc taccaccgtg attttcaaaa cctacaatag ttccaaaagt
    21121 atcactttcg ataacaatat ctcctgttaa tcggtttgtc tttgattcag tataaaaatc
    21181 taaaatacct aacccttcta attctgtacc atcaggcgtg atatattttt ttcctaaaaa
    21241 ttgatagcct ccacaaattg ttaatcccgg cataccatct tcaatcgctt ctttaagtgg
    21301 tgtcttaatt ttacttaatt cttttgttgc taatgcttgt tctctatcac ttccaccacc
    21361 gataaagaaa atatcacatt catcaaaggt aataccttct gtttcattga tttctacgac
    21421 attaacttta atatttcgtt ttttagcacg ttgtcttaaa gcaataatat ttcctatatc
    21481 actgtataaa ttcaatttat ctgacataaa atgataaata gtcaattcat gcatattatg
    21541 attgacctcc ttcaaacgaa cggtttaatt gttcaagcat aggcgctaat gatgtatagt
    21601 ttggtattgc aactgtgaaa cctttataat ccatagtctt tgccgttgct ttataaatat
    21661 cacgctcaac tataattggt acttcaacct ctgctaactt caatcgcaat tgaagttctt
    21721 ctgctcgtgt acctgtcacg atgatagctt caatttgttg cttagataat ttttcaaaat
    21781 ctgcatcata aatccatgaa gtatctcgac catctgcagc gttatcattt agcgaaataa
    21841 catacacttt ttcgccttct aattgttcac caactgataa actcgcattc attcctgcag
    21901 gatttttagc taaattgatc atcgcttctt ttcgttcttt tttaaagtac tgcatacgac
    21961 cattgtctga tgtatacgtt tcaaagccat ttttaattgc ttgttcattt aaccctagct
    22021 ctcttaaaac agtatatgct gctaacgcgt tataagcgtt aaagtcacct gcaattttca
    22081 tatcatattt ttcatcattg atatttaaat gtaaaaacgg tgccacatca aaacttgata
    22141 tttcatattt tgcttgctct cgtttgaaac cacactgaca gtgataatga ccaatttgat
    22201 tataatgaat ataatcgtat tgcaataagc gaccacagtt tggacaatat ctactttcat
    22261 tcatcgtact ttgttcaaat tcatgggcat gtgctttcat accatagtac acaatcgtat
    22321 cacttgcgat tttcaaacga ctcacaaatg gatcatcagc atttagcaat aatttgatgc
    22381 ctttattact aattgtctct gcaatgttat taaccataat atcaatttca ccgaagcgat
    22441 ccatttgatc tctaaagaaa ttagtaaata ccatcattga aggtgtaact tcttttaaca
    22501 cacgtggaat cgaaccttca tcaatttcga ttaccgcaat tttagtctta ggtgttgatt
    22561 gcatgatgaa tgcagaagtt atacctgcag ccatattagc accttcatta ttgtgtataa
    22621 tttgaatatt atttgctttt aaagtatgtc caattaagtt tgaagtcgtt gttttaccat
    22681 ttgttccact gataaataca atatcatcaa cttgctctgc taattttctt aatatatctg
    22741 tatccacttt tctagcgatt tgtccaggta aatctgttcc tcttttacct actgctctac
    22801 ttgctttacg cgccaatttc gctagatgga ttgccgtcca ctgtctcatg tgtttcctcc
    22861 tcaaatttcc actcgcatca ttataacatg acaaggcaac ttcaaaaaag tttctcaatc
    22921 acaaattgat acagtgtttc taaat
//
LOCUS       DAMZKC010000025        22434 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.025, whole genome shotgun sequence.
ACCESSION   DAMZKC010000025 DAMZKC010000000
VERSION     DAMZKC010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 22434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22434
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.025"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            143..2749
                     /gene="yfhO"
                     /locus_tag="POB55_000741"
     CDS             143..2749
                     /gene="yfhO"
                     /locus_tag="POB55_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoteichoic acid-specific glycosyltransferase
                     YfhO"
                     /protein_id="HDI6499725.1"
                     /translation="MLKKWLNSNVKQFFVITFISVILTLILFSTHIYDYIVNGTVFSG
                     AGDGFRQMMPFQMYLYEHLSSFSSLYDASFGLGGDYMKGLSYYYSLSPLMWLNFLFIK
                     IGETVGIFNPTTIHFWPTNQLIMAMIRAIITFVVTFYLFKILHFKRSTNMIATILYGM
                     STVVIYFNFTWSFYGSLLYLLPLSILGLERYFQQRKIGIFIVAIALTLFSNFYFSYYQ
                     AIIIGCYYLYRLIFTYKYDIVSRTQKLICVISATVLSVLSSVFGLFTGISAFLENDRK
                     QNPNVDIPFLTPLDYHYFFFSDGFYITISILTIVALLSFKLYRFYFYRLFAIVTWLLF
                     IGSLSQYFDSAFNGFSFPERRWVYILALSSSALCGLFIQHLSTLNMKYYLIRTIPVSI
                     IALLYVLLSPTHPLALIVGIILLMVLAVILKFSLWRYKKLTVAILVLIVMIQQIVILD
                     NNKNMAIKPYQQSLSTLKQHDYHSNYVNQLIKKINQNATGPFNRIDYMSDYALNSPFI
                     YHYNGISLYSSIFNGDILKYYDKTLQINMPIDKNSTYRLLGNRQNLLSLWNVNDRIRV
                     NHDDNLPYGFKIKSEHKDNKVRWIHSKNTIHYPSAHITNKVFSNKELKSPLDKEQAML
                     QGIVSNKTKDVNTHFKANKNLLSDSTIKLNSAAWQSPTKHLLQVKQNNGGLTVQLPKS
                     VSNQFKDLYFEMDLELLSPDKAHDVKVNEYTQERNKLTYKYRRFVTPVTIRIKASDRI
                     RLSLPKGKYRVNLKGIYGEDYTTLKDASNSLEAVKVSKTKQGYTITKNKNSSGYIVLP
                     TAYNQGMKATSGDQSLKVEQVNGVMTGIKAPKNITKIQLSYAPPYYYLLITITIFGII
                     CSIIFTRWARQK"
     gene            complement(3162..3512)
                     /gene="rplS"
                     /locus_tag="POB55_000742"
     CDS             complement(3162..3512)
                     /gene="rplS"
                     /locus_tag="POB55_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011303276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HDI6499726.1"
                     /translation="MTNHKLIEAVTKSQLRTDLPSFRPGDTLRVHVRIIEGTRERIQV
                     FEGVVIKRRGGGVSETFTVRKISSGVGVERTFPLHTPKIEKIEVKRRGKVRRAKLYYL
                     RSLRGKAARIQEIR"
     gene            complement(3615..4352)
                     /gene="trmD"
                     /locus_tag="POB55_000743"
     CDS             complement(3615..4352)
                     /gene="trmD"
                     /locus_tag="POB55_000743"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HDI6499727.1"
                     /translation="MKIDYLTLFPEMFDGVLNHSIMKRAQENNKLQINTVNFRDYAIN
                     KHNQVDDYPYGGGQGMVLKPEPVFNAMEDLDVTEQTRVILMCPQGEPFSHQKAVELSK
                     ADHIVFICGHYEGYDERIRTHLVTDEISMGDYVLTGGELPAMTMTDAIVRLIPGVLGN
                     EQSHQDDSFSDGLLEFPQYTRPREFKGLTVPDVLLSGNHANIDAWRHEQKLIRTYNKR
                     PDLIEKYPLTNADKQILERYKIGLKKG"
     gene            complement(4352..4855)
                     /gene="rimM"
                     /locus_tag="POB55_000744"
     CDS             complement(4352..4855)
                     /gene="rimM"
                     /locus_tag="POB55_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HDI6499728.1"
                     /translation="MRVEVGQIVNTHGIKGEIKVKSNSDFTDVRFQPGQVLTVVHNNN
                     DLEYTVKSHRVHKGLHMLTFEGINNINDIEHLKGSSIYQERDHEDIVLEENEFYYSDI
                     IGCTVFDDQETPIGRVINIFETGANDVWVIKGSKEYLIPYIADVVKEVDVENKKIIIT
                     PMEGLLD"
     gene            complement(5043..5318)
                     /gene="rpsP"
                     /locus_tag="POB55_000745"
     CDS             complement(5043..5318)
                     /gene="rpsP"
                     /locus_tag="POB55_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HDI6499729.1"
                     /translation="MAVKIRLTRLGSKRNPFYRIVVADARSPRDGRIIEQIGTYNPTS
                     ANAPEIKVDEALALKWLNDGAKPTDTVHNILSKEGIMKKFDEQKKAK"
     gene            complement(5753..7120)
                     /gene="ffh"
                     /locus_tag="POB55_000746"
     CDS             complement(5753..7120)
                     /gene="ffh"
                     /locus_tag="POB55_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002457378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HDI6499730.1"
                     /translation="MAFEGLSERLQATMQKMRGKGKLTEADIKIMMREVRLALLEADV
                     NFKVVKEFIKTVSERALGSDVMQSLTPGQQVIKIVQDELTQLMGGENTSINMSNKPPT
                     VVMMVGLQGAGKTTTAGKLALLMRKKYNKKPMLVAADIYRPAAINQLQTVGKQIDIPV
                     YSEGDQVKPQQIVTNALKHAKEEHLDFVIIDTAGRLHIDEALMNELKEVKDIAKPNEI
                     MLVVDSMTGQDAVNVAESFDDQLDVTGVTLTKLDGDTRGGAALSIRSVTQKPIKFVGM
                     SEKLDGLELFHPERMASRILGMGDVLSLIEKAQQDVDQEKAKDLEKKMRESSFTLDDF
                     LEQLDQVKNLGPLDDIMKMIPGMNKMKGLDKLNMSEKQIDHIKAIIQSMTPAERNNPD
                     TLNVSRKKRIAKGSGRSLQEVNRLMKQFNDMKKMMKQFTGGGKGKKGKRNQMQNMLKG
                     MNLPF"
     gene            complement(7146..7478)
                     /locus_tag="POB55_000747"
     CDS             complement(7146..7478)
                     /locus_tag="POB55_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HDI6499731.1"
                     /translation="MGQNDLVKTLRMNYLFDFYQSLLTNKQRNYLELFYLEDYSLSEI
                     ADTFNVSRQAVYDNIRRTGDLVEDYEKKLELYQKFEQRREIYDEMKQHLSNPEQIQRY
                     IQQLEDLE"
     gene            complement(7465..8715)
                     /gene="ftsY"
                     /locus_tag="POB55_000748"
     CDS             complement(7465..8715)
                     /gene="ftsY"
                     /locus_tag="POB55_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HDI6499732.1"
                     /translation="MSFFKRLKDKFATNKENEEVKSLTEEQGQDKLEDTHSESSTQDA
                     NDLAENAEVKKKPRKLSEADFDDDGLISIEDFEEIEAQKMGAKFKAGLEKSRQNFQEQ
                     LNNLIARYRKVDEDFFEALEEMLITADVGFNTVMTLTEELRMEAQRRNIQDTEDLREV
                     IVEKIVEIYHQEDDNSEAMNLEDGRLNVILMVGVNGVGKTTTIGKLAYRYKMEGKKVM
                     LAAGDTFRAGAIDQLKVWGERVGVDVISQSEGSDPAAVMYDAINAAKNKGVDILICDT
                     AGRLQNKTNLMQELEKVKRVINRAVPDAPHEALLCLDATTGQNALSQARNFKEVTNVT
                     GIVLTKLDGTAKGGIVLAIRNELHIPVKYVGLGEQLDDLQPFNPESYVYGLFADMIEQ
                     NEEITTVENDQIVTEEKDDNHGSK"
     gene            complement(8715..12281)
                     /gene="smc"
                     /locus_tag="POB55_000749"
     CDS             complement(8715..12281)
                     /gene="smc"
                     /locus_tag="POB55_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HDI6499733.1"
                     /translation="MVYLKSIDAIGFKSFADQTNVQFDKGVTAIVGPNGSGKSNITDA
                     IKWVLGEQSAKSLRGSKMEDIIFSGAEHRKAQNYAEVQLRLDNHSKKLSVDENEVIVT
                     RRLYRSGESEYYINNDRARLKDIADLFLDSGLGKEAYSIISQGRVDEILNAKPIDRRQ
                     IIEESAGVLKYKKRKAESLNKLDQTEDNLTRVEDILYDLEGRVEPLKEEAAIAKEYKT
                     LSHQMKHSDIVVTVHDIDQYTNDNRQLDQRLNDLQGQQANKEADKQRLSQQIQQYKGK
                     RHQLDNDVESLNYQLVKATEAFEKYTGQLNVLEERKKNQSETNARYEEEQENLMELLE
                     NISNEISGAQDTYKSLKSKQKELNAVIRELEEQLYVSDEAHDEKLEEIKNEYYTLMSE
                     QSDVNNDIRFLKHTIEENEAKKSRLDSRLVEVFEQLKDIQGQIKTTKKEYQQTNKELS
                     AVDKEIKNIEKDLTDTKKAQNEYEEKLYQAYRYTEKMKTRIDSLATQEEEYTYFFNGV
                     KHILKAKNKELKGIHGAVAEIIDVPSKLTQAIETALGASLQHVIVDSEKDGRQAIQFL
                     KERNLGRATFLPLNVIQSRVVATDIKSIAKEANGFISIASEAVKVAPEYQNIIGNLLG
                     NTIIVDHLKHANELARAIKYRTRIVTLEGDIVNPGGSMTGGGARKSKSILSQKDELTT
                     MRHQLEDYLRQTESFEQQFKELKIKSDQLSELYFEKSQKHNTLKEQVHHFEMELDRLT
                     TQETQIKNDHEEFEFEKNDGYTSDKSRQTLSEKETHLESIKASLKRLEDEIERYTKLS
                     KEGKESVTKTQQTLHQKQSDLAVVKERIKTQQQTIDRLNNQNQQTKHQLKDVKEKIAL
                     FNSDEVMGEQAFQNIKDQINGQQETRTRLSDELDKLKQQRIELNEQIDAQEAKLQVCH
                     QDILAIENHYQDIKAEQSKLDVLIHHAIDHLNDEYQLTVERAKSEYTSDESIDALRKK
                     VKLMKMSIDELGPVNLNAIEQFEELNERYTFLSEQRTDLRKAKETLEQIISEMDQEVT
                     ERFKETFHAIQGHFTAVFKQLFGGGDAELQLTEADYLTAGIDIVVQPPGKKLQHLSLL
                     SGGERALTAIALLFAILKVRSAPFVILDEVEAALDEANVIRYAKYLNELSDETQFIVI
                     THRKGTMEFADRLYGVTMQESGVTKLVSVNLNTIDDVLKEEQ"
     gene            complement(12428..13159)
                     /locus_tag="POB55_000750"
     CDS             complement(12428..13159)
                     /locus_tag="POB55_000750"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HDI6499734.1"
                     /translation="MSKQKKSEIVNRFRKRFDTKMTELGFTYQNIDLYQQAFSHSSFI
                     NDFNMNRLDHNERLEFLGDAVLELTVSRYLFDKHPNLPEGNLTKMRATIVCEPSLVIF
                     ANKIGLNEMILLGKGEEKTGGRTRPSLISDAFEAFIGALYLDQGLDIVWKFAEKVIFP
                     HVEQNELLGVVDFKTQFQEYVHQQNKGDVTYNLIKEEGPAHHRLFTSEVILQGEAIAE
                     GKGKTKKESEQRAAESAYKQLKQIK"
     gene            complement(13275..13508)
                     /locus_tag="POB55_000751"
     CDS             complement(13275..13508)
                     /locus_tag="POB55_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002510960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDI6499735.1"
                     /translation="MENFDKVKDIIVDRLGVDADKVTEDASFKDDLGADSLDIAELVM
                     ELEDEFGTEIPDEEAEKINTVGDAVKFINSLEK"
     gene            complement(13814..14548)
                     /gene="fabG"
                     /locus_tag="POB55_000752"
     CDS             complement(13814..14548)
                     /gene="fabG"
                     /locus_tag="POB55_000752"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HDI6499736.1"
                     /translation="MTKSALVTGASRGIGRSIALQLAEEGYNVAVNYAGSKEKAEAVV
                     EEIKAKGVDSFAIQANVADADEVKAMIKEVVSQFGSLDVLVNNAGITRDNLLMRMKEQ
                     EWDDVIDTNLKGVFNCIQKATPQMLRQRSGAIINLSSVVGAVGNPGQANYVATKAGVI
                     GLTKSAARELASRGITVNAVAPGFIVSDMTDALSDELKEQMLTQIPLARFGQDTDIAN
                     TVAFLASDKAKYITGQTIHVNGGMYM"
     gene            complement(14541..15467)
                     /gene="fabD"
                     /locus_tag="POB55_000753"
     CDS             complement(14541..15467)
                     /gene="fabD"
                     /locus_tag="POB55_000753"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDI6499737.1"
                     /translation="MSKTAIIFPGQGAQKVGMAQDLFNNNDQATEILTSAAKTLDFDI
                     LETMFTDEEGKLGETENTQPALLTHSSALLAALKNLNPDFTMGHSLGEYSSLVAADVL
                     SFEDAVKIVRKRGQLMAQAFPTGVGSMAAVLGLDFDKVDEICKSLSSDDKIIEPANIN
                     CPGQIVVSGHKALIDELVEKGKSLGAKRVMPLAVSGPFHSSLMKVIEEDFSSYINQFE
                     WRDAKFPVVQNVNAQGETDNEVIKSNMVKQLYSPVQFINSTEWLIDQGVDHFIEIGPG
                     KVLSGLIKKINRDVKLTSIQTLEDVKGWNEND"
     gene            complement(15460..16446)
                     /gene="plsX"
                     /locus_tag="POB55_000754"
     CDS             complement(15460..16446)
                     /gene="plsX"
                     /locus_tag="POB55_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HDI6499738.1"
                     /translation="MVKLAIDMMGGDNAPDIVLEAVQKAVEDFKDLEIILFGDEKKYN
                     LNHERIEFRHCSEKIEMEDEPVRAIKRKKDSSMVKMAEAVKSGEADGCVSAGNTGALM
                     SAGLFIVGRIKGVARPALVVTLPTIDGKGFVFLDVGANADAKPEHLLQYAQLGDIYAQ
                     KIRGIDNPKISLLNIGTEPAKGNSLTKKSYELLNQDHSLNFVGNIEAKTLMDGDTDVV
                     VTDGYTGNMVLKNLEGTAKSIGKMLKDTIMSSTKNKLAGAILKKDLAEFAKKMDYSEY
                     GGSVLLGLEGTVVKAHGSSNAKAFYSAIRQAKIAGEQNIVQTMKETVGESNE"
     gene            complement(16451..17023)
                     /gene="fapR"
                     /locus_tag="POB55_000755"
     CDS             complement(16451..17023)
                     /gene="fapR"
                     /locus_tag="POB55_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HDI6499739.1"
                     /translation="MRGETLKLKKDKRREAIRQQIDSNPFITDHELSDLFQVSIQTIR
                     LDRTYLNIPELRKRIKLVAEKNYDQISSIEEQEFIGDLIQVNPNVKAQSILDITSDSV
                     FHKTGIARGHVLFAQANSLCVALIKQPTVLTHESSIQFIEKVKLNDTVRAEARVVNQT
                     AKHYYVEVKSYVKHTLVFKGNFKMFYDKRG"
     gene            complement(17226..19286)
                     /gene="recG"
                     /locus_tag="POB55_000756"
     CDS             complement(17226..19286)
                     /gene="recG"
                     /locus_tag="POB55_000756"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HDI6499740.1"
                     /translation="MAKVNLIESPYSLLQLKGIGPKKIEVLQQLNIHTVEDLVLYLPT
                     RYEDNTVIDLNQAEDQSNVTIEGQVYTAPVVAFFGRNNSKLTVHLMVNNIAVKCIFFN
                     QPYLKKKIELNQTITVKGKWNRVKQEITGNRVFFNSQGTQTQENADVQLEPVYRIKEG
                     IKQKQIRDQIRQALNDVTIHEWLTDELREKYKLETLDFTLNTLHHPKSKEDLLRARRT
                     YAFTELFLFELRMQWLNRLEKSSDEAIEIDYGLDQVKSFIDRLPFELTEAQKSSVNEI
                     FRDLKAPIRMHRLLQGDVGSGKTVVAAICMYALKTAGYQSALMVPTEILAEQHAESLM
                     ALFGDSMNVALLTGSVKGKKRKILLEQLENGTIDCLIGTHALIQDDVIFHNVGLVITD
                     EQHRFGVNQRQLLREKGAMTNVLFMTATPIPRTLAISVFGEMDVSSIKQLPKGRKPII
                     TTWAKHEQYDKVLMQMTSELKKGRQAYVICPLIESSEHLEDVQNVVALYESLQQYYGV
                     SRVGLLHGKLSADEKDEVMQKFSNHEIDVLVSTTVVEVGVNVPNATFMMIYDADRFGL
                     STLHQLRGRVGRSDQQSYCVLIASPKTETGIERMTIMTQTTDGFELSERDLEMRGPGD
                     FFGVKQSGLPDFLVANLVEDYRMLEVARDEAAELIQSGVFFENTYQHLRHFVEENLLH
                     RSFD"
     gene            complement(19476..21122)
                     /gene="fakA"
                     /locus_tag="POB55_000757"
     CDS             complement(19476..21122)
                     /gene="fakA"
                     /locus_tag="POB55_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase catalytic subunit FakA"
                     /protein_id="HDI6499741.1"
                     /translation="MISKINGKLFADMIIQGAQNLSNNADLVDSLNVYPVPDGDTGTN
                     MNLTMTSGREEVENNLSKNIGELGKTFSKGLLMGARGNSGVILSQLFRGFCKNIESES
                     EINSKLLAESFQAGVETAYKAVMKPVEGTILTVAKDAAQAAIEKANNTEDCIELMEYI
                     IVKANESLENTPNLLAVLKEVGVVDSGGKGLLCVYEGFLKALKGEKVEAKVAKIDKDE
                     FVHDEHDFHGVINTEDIIYGYCTEMMVRFGKNKKAFDEQEFRQDMSQFGDSLLVINDE
                     EIVKVHVHTEYPGKVFNYGQQYGELIKLKVENMREQHREVIRKEQHTAKPKMETVETA
                     IITISMGEGISEIFKSMGATHIISGGQTMNPSTEDIVKVIEQSKCKRAIILPNNKNIL
                     MASEQAASIVDAEAVVIPTKSIPQGISALFQYDVDATLEENKAQMADSVNNVKSGSLT
                     YAVRDTKIDGVEIKKDAFMGLIEDKIVSSQSDQLTTVTELLNEMLAEDSEILTVIIGQ
                     DAEQAVTDNMINWIEEQYPDVEVEVHEGGQPIYQYFFSVE"
     gene            complement(21137..21511)
                     /locus_tag="POB55_000758"
     CDS             complement(21137..21511)
                     /locus_tag="POB55_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDI6499742.1"
                     /translation="MTLEISNDYGKIDISNEVIASVVGGKAVECYGIVGMASRQQVRD
                     GIAEILGHENYAKGIKVTENNGVVDIDMYIIVSYGVKISEVANNVQSTVKYTLEKSLN
                     VSVNSINIYVQGVRVNNTGKKA"
     gene            21954..22142
                     /locus_tag="POB55_000759"
     CDS             21954..22142
                     /locus_tag="POB55_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014614133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HDI6499743.1"
                     /translation="MGKQCFVTGRKASTGNRRSHALNSTKRRWNANLQKVRILVDGKP
                     KKVWVSARALKSGKVTRV"
BASE COUNT         6787 a         4325 c         3031 g         8291 t
ORIGIN      
        1 ataaactgaa aatgatccaa agtttaagta actatgataa ggtatctgtt ccacagtcat
       61 cttcgtcttt acctatcaag aacctcatta actttagttt cagttaaact taattttaaa
      121 tttgaaagtt gaggataatg ttgtgttaaa aaagtggcta aattcaaacg tcaaacaatt
      181 ctttgttata actttcatta gtgtaatatt aacgcttatt ttattttcta ctcatatcta
      241 tgattatatt gtgaatggta ctgtttttag tggggctgga gatggattcc gtcaaatgat
      301 gccatttcaa atgtatttgt atgaacattt aagtagtttt tctagtttat atgatgcatc
      361 gtttggatta ggtggcgatt atatgaaagg actatcatat tattattcgc tgtcaccttt
      421 aatgtggcta aattttctat tcattaaaat aggagaaacg gttggtatat ttaatccgac
      481 aacaatacat ttttggccga caaaccaact tattatggcc atgataagag ctatcataac
      541 atttgtcgtg accttctact tatttaaaat attacacttt aaacgctcaa caaatatgat
      601 cgctacgatt ttatacggca tgtcaactgt cgttatatac tttaatttta cttggtcatt
      661 ttatggaagt ttattatatt tattaccatt atcgattctt ggtttggaaa gatattttca
      721 acaacgcaaa atcggtattt tcattgttgc gatagcctta acactattta gcaattttta
      781 tttcagttat tatcaagcta ttattatagg ttgctactat ttatatcgac tcattttcac
      841 ttacaaatat gacattgtct ctagaacaca aaaattaatt tgcgtcatat ctgctacagt
      901 tttgagtgtg ttatcaagtg tatttggttt attcactggc atttctgcgt ttttggaaaa
      961 tgacagaaag caaaatccca atgttgatat accgtttttg acaccacttg attaccatta
     1021 ttttttcttt agcgatggat tttatattac gatttcaatt cttactatcg ttgcattatt
     1081 gtcattcaaa ctgtatcgtt tttactttta tagacttttc gcaatagtaa catggttatt
     1141 atttattggt tcattatcac agtatttcga cagtgctttt aatggttttt catttccaga
     1201 aaggcgttgg gtgtatatct tagcactatc atcaagcgct ctttgcggat tgtttattca
     1261 acatttatca acattaaata tgaaatatta tttaatcaga acaataccag taagcatcat
     1321 cgcattactt tatgtattac tatcaccgac acacccactt gcacttatag taggtattat
     1381 cctgctaatg gtgcttgccg ttattttaaa atttagttta tggcgttata aaaaattaac
     1441 cgttgcaata ttagtattaa tcgttatgat tcaacaaatc gtcattttag ataacaataa
     1501 aaatatggca atcaaacctt atcaacaatc attatcaacg ttgaaacaac atgattacca
     1561 tagtaactat gtaaaccagc ttataaaaaa gataaatcaa aatgcaactg gtccatttaa
     1621 tcgcattgat tatatgtcag actatgcatt aaattcacca tttatatatc attataatgg
     1681 catttcatta tattctagta tttttaatgg agacatatta aaatattatg acaagacact
     1741 ccaaattaat atgccaatcg ataaaaacag cacttataga ttacttggca atcgtcaaaa
     1801 tttactatca ctttggaatg ttaatgatcg aattagagtg aatcatgatg acaacttacc
     1861 atatggattt aaaattaagt ctgaacacaa agacaataaa gttcgttgga ttcattctaa
     1921 aaataccatc cattatccaa gtgcacatat tacaaataag gtcttttcca ataaagaatt
     1981 aaaatctcca ttagataaag aacaagcaat gttgcaaggg attgtttcta acaagactaa
     2041 agatgttaat acacatttta aagccaataa aaatttacta tcagattcaa caattaaatt
     2101 aaatagtgca gcctggcaat ctcctacaaa acatttatta caagttaaac aaaataatgg
     2161 tggtctaact gtacagttgc caaaatcagt ttctaatcaa tttaaagatt tgtattttga
     2221 aatggattta gaattacttt cgccggataa agctcatgat gttaaagtga atgaatatac
     2281 acaagaaaga aataaactca cttataaata tcgacgcttt gtaacacccg taacaatacg
     2341 cattaaagct tcagatagga ttagattatc attgcctaaa ggtaagtatc gtgtaaattt
     2401 aaaagggata tacggcgaag attataccac gcttaaagac gcttcaaatt cattagaggc
     2461 tgtcaaagtt agtaagacaa agcaaggtta tactattact aaaaataaaa attcatctgg
     2521 gtatattgtt ttgccaacag catataatca aggtatgaaa gcgacatcag gtgatcaaag
     2581 tcttaaagtt gaacaagtaa atggtgttat gaccggcatt aaagcaccta aaaatataac
     2641 aaagattcaa ttgagctatg ccccaccata ctattattta cttataacaa ttactatatt
     2701 tggcattata tgtagtatta ttttcacgag atgggcaaga caaaaataat gtagctacgc
     2761 atatatttaa tgggagtggg acagaaatga tattttcgca aaatttattt cgtcgtccca
     2821 ccccaacttg cacattattg taagctgact tttcgtcagc ttctgtgttg gggcccctga
     2881 ctagaattga aaaaggcttg ttacaagtgc attttcgttc agtcaactac tgccaatata
     2941 acttcgtaga gatagaacat tgatttatgt cccagccttt tgtttatata accattttga
     3001 gcaaacacgt tatgaataga gaaggaataa acaattacat tatcaacttt acattaacta
     3061 aacagcaaaa aaagccatct atatcactcc ataaaagtga cctagatggc tttattgttt
     3121 gagtcgaatt tgactcatac atagctttgt ttaaatgctg attaacgaat ttcttggatt
     3181 ctagcagctt taccacgtaa actacgtaag taatataatt tagcacgacg tactttacca
     3241 cgacgtttaa cttcgatttt ttcaattttt ggtgtgtgta atgggaatgt acgttccacg
     3301 ccaacacctg atgaaatttt acgaactgta aacgtttcag aaacgccacc gccacgacgt
     3361 ttaattacaa cgccttcgaa tacttggata cgctcacgag taccctcaat gatacgtacg
     3421 tgtacacgta aagtatcacc aggacggaaa ctaggtaagt ctgtacgtaa ttgtgattta
     3481 gttactgctt cgattaattt gtgatttgtc attataaatt ctcctcttca acctatgttc
     3541 ttgcctcgac aaatatatag cagcggatca tagtgatttt tgtgtttcca cactcagatt
     3601 atgttagcac aatactaacc ttttttcaat cctattttat atctttctaa tatttgctta
     3661 tctgcattag ttaatggata tttttcaatt aagtcaggtc ttttattata tgtgcggatc
     3721 aacttttgct catgtctcca tgcatcaata ttggcatgat ttccagacaa taaaacatct
     3781 ggaactgtta gacccttaaa ttcacgcgga cgtgtatatt gcggaaactc taataaccca
     3841 tctgaaaatg aatcgtcttg atgtgactgt tcattaccta aaacacctgg aatcagtcta
     3901 acaatagcat cagtcatggt catcgctggc aattctccac cagttaaaac atagtcaccc
     3961 attgatattt catctgtgac aagatgtgtt cggatacgtt catcgtaacc ttcataatgt
     4021 ccgcatatga aaacgatgtg gtcggccttg cttaattcaa cagctttctg atgtgaaaat
     4081 ggctcgcctt gtggacacat taaaataacg cgtgtttgtt ctgtgacatc taagtcttcc
     4141 atcgcattaa aaacaggttc aggctttaac accatacctt gtccgccacc atacggataa
     4201 tcatctactt ggttgtgctt gttaattgca taatctctaa aattaaccgt attgatttgt
     4261 aatttattgt tttcttgggc acgtttcata attgaatgat ttaaaacacc atcaaacatt
     4321 tcaggaaata aagttaaata atcaattttc attaatccaa caatccttcc attggcgtga
     4381 tgataatttt tttattttca acatccactt cttttacaac atcagcaata taaggaatca
     4441 aatattcttt agatccttta atcacccaca catcattcgc acctgtttca aatatattaa
     4501 ttacacgacc tattggtgtt tcttgatcat caaaaactgt acatcctata atatctgaat
     4561 aataaaattc attttcctca agtacgatat cttcatgatc acgctcttga taaatagaac
     4621 tcccttttaa atgctcaata tcattaatat tattaatacc ttcaaatgta agcatatgaa
     4681 gccctttatg cactctatgt gacttaacag tatattcaag gtcgttattg ttatgcacaa
     4741 ctgtcagcac ttgaccgggt tgaaaacgaa cgtctgtaaa gtctgaattg gattttactt
     4801 taatttcacc tttaatacca tgtgtgttaa caatttgacc aacttcaact ctcattctat
     4861 tacctccaac tctatgcttt gaacacttct attttaattg aaaataattc aaagaaaaag
     4921 ggcaccatta tggtacccaa tacgttctat attactaaca tgtgatattc ccaacgtatc
     4981 attgagtaat aactgtcagt gtaaacgagt tattcttatt gttagaacaa ttttacgcta
     5041 aattacttag ctttcttttg ttcgtcaaat tttttcataa taccttcttt tgataagata
     5101 ttgtgaactg tatcagttgg tttcgcacca tcatttaacc attttaaagc taacgcttcg
     5161 tcaactttaa tttctggagc attagcgctc gttgggttat aagtaccgat ttgttcgatg
     5221 atacgtccgt cacgtggaga acgagcatct gctactacga tacgatagaa tggatttctt
     5281 tttgaaccta aacgtgttaa acgaatttta actgccattt ataaaatctc ctttaagtca
     5341 tgttttaatt tttattttct acaagaaata atattaacag gtgtgtgaat gtttgtaaag
     5401 agttttttct ttaccaggaa aattattgaa cctttttctt acaataataa ctatgtgatt
     5461 actaaattag actgttaata ggttataaaa attcatagta gccttcacca catacgtgtt
     5521 tagttaattt atcccgtaag gacatgaatg gtaccacttc taccgcacac attcccccaa
     5581 tataacttat taatattagc aacaataacc tcatgttcta gcaaatccgc aatttcagtt
     5641 tttaattatt attgtgtatc taaaaatatg caccccataa agttatattc tttagtataa
     5701 ctttcgggat gcacatctca ttgcaacaaa aatcagcgtg ttatattaaa cattaaaacg
     5761 gtaaattcat accttttaac atattttgca tttgattgcg tttacctttt ttacctttac
     5821 cgccaccagt gaattgtttc atcattttct tcatatcgtt aaattgtttc atcaaacgat
     5881 tgacttcttg taatgaacga ccagaccctt tagcaatacg ctttttacgt gatacattca
     5941 atgtgtctgg attgtttctt tcagccggcg tcattgactg gataatcgct ttaatatgat
     6001 caatttgctt ttcactcata ttaagcttat ctagcccttt cattttattc atacctggaa
     6061 tcattttcat aatatcatcc agtggtccta gatttttcac ctgatcaagt tgttctaaaa
     6121 aatcatctaa agtaaacgat gactcacgca tctttttctc taaatctttt gctttttctt
     6181 gatccacatc ttgttgcgct ttttcaatta aacttaacac atcacccata cctaaaatac
     6241 gtgatgccat acgttcagga tggaatagct ctaaaccatc taacttttca ctcataccaa
     6301 caaatttaat tggtttttgt gtcaccgaac gaatagataa agctgcacca ccacgtgtat
     6361 caccatctaa tttagttaag gtaacacctg tgacatcaag ttgatcgtca aaagattctg
     6421 caacattgac agcatcttga cccgtcattg aatcgacaac taacataatt tcgtttggtt
     6481 tagcaatgtc ttttacttct tttaattcgt tcatcaatgc ttcatcgatg tgtaatcgac
     6541 ctgctgtatc aatgattaca aagtctaaat gttcttcttt agcatgtttt aatgcattag
     6601 ttacaatttg ttgtggcttt acttgatctc cttcactgta tacaggaata tcaatttgtt
     6661 tccctactgt ttgtaattga tttatcgctg ctggacgata aatatctgct gcaactaaca
     6721 taggtttttt gttgtatttt ttacgcatca ataatgctaa tttacctgca gttgttgttt
     6781 taccagcacc ttgtaaacca accatcataa caacagtagg tggtttattt gacatattaa
     6841 tcgacgtatt ttctccaccc atcaactgcg ttaattcatc ttgaactatt ttaataactt
     6901 gttgccctgg tgttaatgat tgcattacat cggaacctaa tgcgcgttct gatactgttt
     6961 taataaattc ttttaccact ttaaagttta cgtcagcctc aagtaacgct aatcttactt
     7021 cacgcatcat tatctttata tcagcttcag taagtttacc cttaccacgc attttttgca
     7081 tcgtcgcttg caagcgttct gataaccctt caaatgccat atcgatgtcc tccctcaatt
     7141 tatatctatt ctaagtcttc taattgttga atataacgtt gtatttgttc tggattactt
     7201 aaatgttgtt tcatttcatc atatatttct cggcgttgct caaatttctg gtataattcc
     7261 aatttctttt cataatcttc aactaaatcg ccagttcttc ttatattatc ataaactgct
     7321 tgtctactca cattaaaagt atctgcgatt tcacttaaag aataatcttc aagataaaat
     7381 aattccaaat aattacgttg tttattcgtc aataaggatt gataaaaatc aaacaaataa
     7441 ttcattcgta acgttttaac taaatcattt tgacccatga ttatcgtcct tttcttctgt
     7501 tacaatttga tcattttcaa ctgttgttat ttcttcattt tgttcaatca tatcagcgaa
     7561 taagccgtag acataacttt cagggttaaa tggttgtaag tcatctaatt gctcacctaa
     7621 acctacatat ttaactggaa tgtgcaattc attacgaatg gctaatacga taccaccttt
     7681 ggctgtacca tctaatttcg ttaatacaat acctgtaaca tttgttactt ctttaaagtt
     7741 tctagcttgt gacaacgcat tctgaccagt tgtagcatct aaacatagta atgcttcatg
     7801 aggcgcatct ggcactgctc gattaattac acgcttaact ttttctaatt cttgcattag
     7861 atttgtttta ttttgtaaac gtccagcggt atcacagatt aaaatatcaa cacctttgtt
     7921 tttagcggca ttaatcgcat catacataac agcagctgga tcagaacctt cactttggct
     7981 aattacgtct acaccaacac gttcgcccca aactttcaat tgatcaatag cacccgctct
     8041 aaaagtatcg cccgcagcta acattacttt tttaccttcc attttatatc ggtaagctaa
     8101 ttttccaatt gttgttgttt taccaacacc attcacacca accattaaaa tgacatttaa
     8161 acgaccatct tctaagttca tagcttctga attatcatct tcttgatggt aaatctctac
     8221 gattttttca acaatgactt cacgcaaatc ttcagtatct tgaatattac gtcgttgtgc
     8281 ttccatacgt aattcttcag ttaacgtcat cactgtatta aaaccgacgt ctgcagtgat
     8341 taacatttct tctaaagctt caaaaaagtc ttcatctact ttacgatatc tcgctatcaa
     8401 attatttaat tgttcttgga aattttgacg agatttttcg agtcctgctt taaatttagc
     8461 acccattttt tgagcttcaa tttcttcaaa atcttcaatt gatattaagc catcgtcatc
     8521 aaaatccgct tcactcaact tgcgtggctt ctttttcact tcagcatttt ctgctaaatc
     8581 atttgcgtcc tgcgttgaac tttcagaatg tgtatcttct aatttgtctt gaccttgttc
     8641 ttctgttaag gatttaactt cttcattttc tttatttgtt gcaaacttat cttttaagcg
     8701 tttaaaaaag ctcattattg ctcctccttc aacacatcat ctattgtatt taaattcaca
     8761 ctcacaagtt tagtaacacc tgattcttgc attgttacac cgtataacct atccgcaaat
     8821 tccattgttc ctttacggtg tgtaataaca atgaattgtg tttcgtctga taactcattt
     8881 aaatattttg cgtatctaat aacatttgct tcatctagtg cagcttcaac ctcatctaat
     8941 ataacaaaag gtgcagatct tacttttaaa attgcaaata gtaaagcaat agcagttaat
     9001 gcacgctcac caccactcag taacgataaa tgttgcaact ttttacccgg tggttgtacc
     9061 acaatatcaa taccagctgt taaataatcg gcttcagtta attgcaattc tgcatcgcct
     9121 ccaccaaaca attgtttgaa cacagctgta aaatgtcctt gaatagcatg gaaagtttct
     9181 ttaaatcttt cagtaacctc ttgatccatt tcacttataa tttgctctaa tgtttcttta
     9241 gctttacgaa gatctgtacg ttgttcactt aaaaatgtat aacgttcatt taactcttca
     9301 aattgttcaa ttgcatttaa gtttacagga cctagttcat caatcgacat cttcattaac
     9361 ttaacttttt tacgtaatgc gtcaatcgat tcatcactcg tatattcaga tttcgcacgt
     9421 tcaacagtca attgatattc atcatttaaa tgatctatcg catgatgaat taatacatct
     9481 agctttgatt gttcagcttt aatatcttgg tagtgatttt cgatagctaa aatatcttgg
     9541 tgacaaactt gtagtttagc ttcttgcgca tcgatttgtt cattcaactc aatacgttgt
     9601 tgtttcaatt tatctaattc atcagataag cgtgttctcg tttcttgttg accattaatt
     9661 tgatctttaa tattttgaaa agcttgttcg cccatcactt catccgaatt aaacaatgca
     9721 attttttctt taacatcttt taattgatgt ttagtttgtt gattttgatt atttaatcga
     9781 tctattgtct gttgttgtgt tttaatacgc tctttaacca cagcaagatc agattgtttc
     9841 tgatgtaacg tttgttgtgt tttagttacg ctttccttac cttctttaga aagttttgtg
     9901 tagcgttcaa tttcatcttc tagtcgtttt aaagatgctt taatactttc taaatgagtt
     9961 tctttttcac tcaaagtttg tcgacttttg tcactcgtat aaccatcatt tttttcaaat
    10021 tcgaattctt catgatcatt ttttatttgt gtttcttgtg tagttaatct atcgagctcc
    10081 atttcaaaat gatgcacttg ctctttaagt gtattatgct tttgactttt ttcaaaatac
    10141 agttcactta attgatcact ttttatcttc aactctttaa attgttgttc aaatgattct
    10201 gtttgacgca agtaatcttc taattggtgt ctcattgttg tcaactcgtc tttttgagac
    10261 agaatacttt tggacttacg agcgccacca ccagtcatag aaccaccagg gtttacaata
    10321 tcaccttcca aagtaacaat acgagttcga tatttaatcg cacgtgccaa ttcatttgca
    10381 tgctttaaat gatcaacgat aatcgtatta cctaataaat tcccgataat attttgatat
    10441 tctggtgcta ctttaactgc ttccgaagca atactaataa atccgtttgc ctctttagca
    10501 atagatttaa tatcagtcgc taccactcta ctctgtataa catttaatgg taaaaacgtc
    10561 gcacgaccta aattacgttc ttttaaaaat tgaatagcct ggcgtccatc tttttctgaa
    10621 tctacaatga catgttgtaa tgaagcacct aatgctgttt caattgcctg agttaattta
    10681 gatggcacat caataatttc cgcaactgca ccatgaatac cctttaattc tttattttta
    10741 gctttcaaaa tatgtttgac gccattgaaa aaataagtat attcttcctc ttgcgttgcc
    10801 aaactatcaa tacgtgtttt cattttttcg gtatatcgat atgcttgata caatttctct
    10861 tcgtattcat tttgtgcttt ttttgtatca gtgagatctt tttctatatt tttaatttct
    10921 ttatctacag cagaaagttc tttgttggtc tgttgatatt ctttttttgt cgtttttatt
    10981 tgaccctgaa tatctttcaa ttgctcaaaa acttcaacta atcgagaatc tagtcttgat
    11041 tttttagcct cattctcttc tatagtatgc tttaaaaaac gaatatcatt gttaacatct
    11101 gattgctctg acattaatgt atagtattcg tttttaattt cttccaattt ttcatcatgt
    11161 gcttcgtctg aaacataaag ttgttcttca agttcacgaa tgacagcatt gagttctttt
    11221 tgtttacttt tcagagactt ataagtatct tgagctccag aaatctcatt tgatatattt
    11281 tctaaaagct ccattaaatt ttcttgttct tcttcatatc gtgcatttgt ttcagattga
    11341 tttttcttac gttcttctaa aacatttaat tgtcccgtat atttttcaaa ggcttccgta
    11401 gcttttacta attgataatt aagcgattca acatcattat caagttgatg acgtttacct
    11461 ttatattgtt gaatttgttg gcttaaacgt tgtttgtcag cttctttatt tgcttgttgg
    11521 ccttgtaaat catttaaacg ttgatctaat tgtctattgt catttgtata ttgatcaata
    11581 tcgtgcactg taactacaat gtcactatgt ttcatttgat gtgaaagtgt cttatattct
    11641 ttagctatag ctgcctcctc ttttagaggt tctacacgac cttccaaatc atataaaatg
    11701 tcttctactc tcgttaaatt atcttctgtt tggtcaagtt tatttaatga ttcagcttta
    11761 cgttttttat atttaagtac accagccgat tcttcaataa tttgacgtct atcaattggt
    11821 ttagcattta gtatttcatc aactctacct tgcgagataa tgctatacgc ttcttttccc
    11881 aatccagaat ctaaaaataa atcggcaata tcttttaatc ttgcacggtc attatttatg
    11941 tagtactcac tttcaccact tcgatacaat cttcttgtta caataacttc gttttcatca
    12001 acactgagct ttttagaatg attatctaat cttaactgta cttcagcata attttgagct
    12061 ttgcgatgtt ctgcacctga gaagataata tcttccattt ttgagccacg taatgattta
    12121 gccgattgtt cacccaacac ccatttaata gcatctgtaa tattactttt accgcttcca
    12181 tttggaccaa caattgcagt tacaccttta tcgaattgaa cattggtttg atctgcaaaa
    12241 gacttaaatc caatggcatc tattgatttt aaataaacca tcctaaactc cttatcacaa
    12301 ttttaaagaa cacatgtata cgatatttta gtatttgtat attaaatttc gaagttgttc
    12361 cataaaatat taaaaattct tccttaaatc ctaaatcgaa cacctatatg tcgtatcata
    12421 taaatttcta tttaatttgt tttaattgct tataggcact ttcagcagca cgttgttctg
    12481 attctttttt cgttttccct ttaccttcag ctattgcttc cccttgcaga ataacttctg
    12541 aagtgaatag acgatgatgt gccggtccct cttcttttat taaattatag gttacatcac
    12601 ctttattttg ctggtgcaca tattcttgga attgtgtttt aaaatctacc acgcctaata
    12661 actcattttg ttctacatgt gggaaaatga ctttctcagc aaatttccaa actatatcta
    12721 gtccttgatc caaatacaat gccccaataa atgcttcgaa tgcatctgat attaatgatg
    12781 gtcttgtacg tccccctgtt ttctcttcac ctttaccaag taaaatcatt tcgttcaatc
    12841 caattttatt cgcaaatatt acaagtgagg gctcacatac aatagtggca cgcatttttg
    12901 ttaaattccc ttctggcaag ttgggatgtt tatcaaataa atatcgtgaa accgtcaatt
    12961 ctaataccgc atcacccaaa aactctaaac gctcattatg gtctaaacga ttcatattaa
    13021 aatcattaat aaaactcgaa tgcgaaaatg cttgttggta taaatcaata ttttgataag
    13081 taaagcctaa ctctgtcatt ttagtatcaa agcgctttct aaaacgatta actatctcac
    13141 ttttcttttg tttagacatt attttatctc ctttatgtgt tgctcttgaa tcatctttaa
    13201 ttttacgtta aaactattga taattaaaga aaaaaactga gtcgacaata ctgacgaccc
    13261 agatgtaaga tttattattt ttcaagactg ttaataaatt taacagcatc accaacagtg
    13321 ttgatttttt cagcttcttc atcaggaatt tcagtaccaa actcgtcttc taattccatt
    13381 actaattcag cgatatcaag tgagtcagcg cctaaatcat ctttgaaaga tgcatcttca
    13441 gttactttat cagcgtctac acctaaacgg tcaacgatga tatcttttac tttatcgaaa
    13501 ttttccacgt cgattcacct cctttaaaaa tgcctataca tagactatta tattttccca
    13561 ttttaaattc taaaaaacaa gtagaaattt tagaaacact tatcaattta ttgattagtg
    13621 tttcttaggg agaaattctc gaaccttatc taggttgctt agaatttccc ccgcaaagtt
    13681 gactaggtca ggcaattctc cattggatga ccagtcaacc actgcgtcaa ttagctttag
    13741 ctcaggcgtt ccgcttatca gttatcaatt tattgattac tacgtcttta tcttgagctt
    13801 tagctcaaat atattacatg tacattccac catttacatg gattgtttga cctgtaatat
    13861 attttgcttt gtctgatgct aagaacgcta ctgtattagc aatatctgtg tcttgaccaa
    13921 aacgtgctaa cggaatttga gtcaacattt gttctttaag ctcatcactt aaagcatctg
    13981 tcatatcaga aacaataaaa ccaggtgcaa ctgcatttac agtgatacca cgagatgcta
    14041 attcacgcgc cgcagattta gttaaaccaa taacacctgc ttttgttgca acatagtttg
    14101 cttgtcccgg attacctact gctccaacaa cacttgataa attgatgata gcaccactac
    14161 gttgtcttaa catttgtggt gttgcttttt ggatacagtt aaatacacct tttaagtttg
    14221 tgtcaataac atcatcccac tcttgttctt tcatacgcat taataaatta tcgcgagtaa
    14281 tacctgcatt atttactaaa acatctaaag aaccaaattg gctaactact tctttaatca
    14341 ttgctttaac ttcatcagca tcggcaacat ttgcttgaat cgcaaaactg tcaacacctt
    14401 tagctttgat ttcttcgact actgcttcag ctttctcttt gctgcctgca tagtttactg
    14461 ctacattata tccttcttct gctaattgta acgcaatact acgtccaatt cctcttgatg
    14521 cacctgttac taaagcactc ttagtcattt tcattccatc ctttcacatc ttctaaagtt
    14581 tgaattgatg ttaacttaac atctctattt atttttttaa ttaagccaga taaaactttt
    14641 ccaggaccaa tttcaataaa atgatcaaca ccttggtcta ttagccattc tgttgagtta
    14701 atgaattgta ctggtgaata taattgcttg accatattag atttaattac ttcattgtca
    14761 gtttcacctt gcgcatttac attttgaact acaggaaact tagcatcacg ccattcaaat
    14821 tgattaatat aacttgaaaa atcttcttca atcactttca ttagcgatga atggaatgga
    14881 ccagatactg ctaaaggcat gacacgtttt gcacctaatg atttaccttt ttctactagc
    14941 tcatcaatta aagctttgtg acctgaaaca acaatttgac ctgggcaatt aatgtttgct
    15001 ggttcaatta ttttgtcatc agatgataat gacttacaaa tttcatcgac tttatcaaaa
    15061 tctaagccca atactgcagc catacttcct acaccagtag gaaatgcttg cgccattaat
    15121 tgaccacgtt ttctaacaat tttaactgca tcttcaaatg ataatacgtc agctgcaact
    15181 aaacttgaat attcacctaa actatgcccc atagtaaaat caggattcaa attttttagc
    15241 gctgctaata atgccgaact atgcgtcaat aaagctggtt gcgtgttttc agtttcaccc
    15301 aatttacctt cttcatcagt aaacattgtc tctaaaatat caaagtctaa cgtctttgct
    15361 gctgaagtta aaatttcagt tgcttgatca ttgttattaa acaaatcttg tgccatacca
    15421 actttttggg caccttgtcc cggaaaaata attgctgttt tactcatttg attcacctac
    15481 agtctctttc attgtttgta caatattttg ttctcctgcg attttcgctt gtctaattgc
    15541 agaataaaaa gctttagcat ttgaactacc gtgtgcttta actacagtac cttccaatcc
    15601 taataatact gaaccaccgt attctgagta atccatcttt ttagcgaatt ctgctaaatc
    15661 tttcttcaat attgcacctg ctaatttatt tttagtacta ctcataatcg tatcttttaa
    15721 cattttaccg attgattttg cagtaccttc taaattttta aggaccatgt tcccagtata
    15781 gccatcggta actacaacat ctgtatcgcc atccattaat gtcttcgctt caatattccc
    15841 aacaaaattc aatgaatgat cttgatttaa taactcatat gattttttcg ttaaactatt
    15901 acctttagct ggctcggtac ctatatttaa taatgagatt ttcggattat caatacctct
    15961 gattttttga gcataaatat cccctagttg cgcatactgt aataagtgtt caggtttagc
    16021 atcagcattt gcaccaacgt ctaaaaatac aaaacctttt ccatcaatcg ttggcaatgt
    16081 tactactaaa gccggtctag ctacaccttt aatacgtcca acaatgaata aaccagctga
    16141 cattaaagca ccagtattac ctgctgacac acatccatct gcttcaccag atttcacagc
    16201 ttcagccatt tttaccattg agctatcttt tttacgttta atcgctctaa caggctcatc
    16261 ttccatttca atcttttcag aacaatgtct aaattcgatt cgttcatggt tcagattata
    16321 ctttttttcg tcaccgaaaa gtataatttc tagatcttta aagtcttcaa cagccttttg
    16381 tacggcttct aatacgatat caggcgcatt gtcgccaccc atcatatcaa ttgctaattt
    16441 aaccataatt ttatcctcgc ttatcataaa acattttaaa atttcctttg aaaactaatg
    16501 tatgtttaac atatgacttt acttcgacgt aataatgttt tgcagtttga tttacaactc
    16561 gtgcttctgc tcttaccgta tcatttaatt ttactttttc aataaattga atgctactct
    16621 catgagttaa aactgttggt tgcttaatta gcgcaacaca taacgaattt gcctgagcaa
    16681 acagcacatg accacgcgca attccagttt tatgaaaaac agaatccgat gtaatatcta
    16741 aaattgattg cgctttaaca tttggattga cttgaatcaa atcaccaata aattcttgtt
    16801 cttcaataga acttatttgg tcataattct tttcagcaac taatttaata cgcttcctta
    16861 attctggtat gtttaaataa gtgcgatcta aacgaattgt ttgtatactc acttgaaata
    16921 agtcgcttag ttcatggtct gtgatgaagg gattgctatc aatttgttgt ctgattgctt
    16981 ctctacgttt atctttcttt agtttcaacg tctcacccct cattttttag tacctagtct
    17041 taaacattcc tcacatatca taacactttc tatttaaccg actcaacaaa taactgtatt
    17101 atttagctat tatttagtcg gaaatttata aattgttaaa ataccacttt acatttaaat
    17161 agttcaagtg tcatatcact caattaacga agtgttgcac tcaaattgac aaatcagcat
    17221 ggtaattagt caaaactgcg atgtaataaa ttttcttcaa caaaatgacg taaatgttga
    17281 tacgtatttt caaagaatac gccagattga ataagttcag ctgcttcatc acgagcaact
    17341 tctaacatac gataatcttc aactaaattg gcaactaaga aatctggcaa cccactttgt
    17401 ttaacaccaa agaaatctcc aggaccacgc atttctaagt ctcgttcact caattcaaat
    17461 ccatccgttg tttgtgtcat aattgtcatt ctttcaattc ctgtttctgt tttaggggat
    17521 gcaattaaaa cacagtaact ttgctggtca cttctaccta cacgaccgcg taactgatgt
    17581 aaagttgata atccaaagcg atccgcatca taaatcatca taaaagttgc attcggtaca
    17641 ttaacaccta cttcaacaac agtagtagaa actaaaacat ctatctcatg attactaaac
    17701 ttttgcatga cctcatcttt ttcatcggca gataacttac catgcaataa ccctacacgg
    17761 gaaacaccat aatactgttg taaagactcg tacaatgcga caacattttg aacatcttcg
    17821 agatgctcag aactttctat tagcgggcaa atgacatatg cttgacgacc ttttttcaac
    17881 tctgaggtca tttgcatcaa aactttatcg tattgctcat gctttgccca agtagtaatg
    17941 ataggtttac gaccttttgg taattgttta attgaagaca catccatctc accaaaaact
    18001 gatattgcta gtgttcttgg tatcggggtt gctgtcataa ataacacatt cgtcattgca
    18061 cctttttctc ttaaaagctg gcgttgattc acaccaaatc gatgttgttc atctgtaatt
    18121 actaaaccaa cattatggaa aatcacatca tcttgaatca aagcatgggt tccaattaaa
    18181 caatcaatcg taccattttc aagttgttct aaaagtatct ttcgtttctt accttttact
    18241 gacccagtta acaatgcaac gttcatagaa tctccaaata aagccattaa actttcagca
    18301 tgttgctctg ctaaaatttc agttggtacc atcaatgctg attgataacc agccgttttt
    18361 aacgcataca tacaaattgc agcaactact gtttttcctg aacctacatc accttgaagt
    18421 aatcgatgca tacgtattgg tgcttttaaa tctctaaaaa tttcattaac actggatttc
    18481 tgtgcttcag ttagttcaaa aggtaaacga tcaataaatg atttaacttg gtctaagcca
    18541 taatcaattt caattgcttc gtcagatgac ttttctaatc tatttagcca ttgcatacgt
    18601 aattcgaata aaaacagttc agtaaatgca taggttctac gagcacgtaa taaatcctct
    18661 ttacttttag gatgatgtaa tgtgttcaaa gtaaagtcca aggtctctaa tttatatttt
    18721 tctcttagtt catcagttaa ccattcatga attgtcacat catttaacgc ttgtctaatt
    18781 tggtctcgta tttgcttttg tttaatacct tccttaatac gatagactgg ttctaattga
    18841 acatctgcgt tttcttgagt ttgtgtccct tgtgaattaa agaaaaccct attaccagta
    18901 atttcctgtt taactctatt ccacttacct ttaacagtta tagtttgatt taattcgatt
    18961 ttctttttta aatacggttg attgaaaaaa atacatttga cagcaatatt atttaccatt
    19021 aaatgaacgg ttaattttga attatttctt ccaaaaaatg caactactgg agctgtatat
    19081 acttgtcctt ctatcgtaac gttagattga tcttctgctt gattcaaatc aatcactgta
    19141 ttatcttcat atctagttgg caaataaaga acaagatctt ccactgtatg aatatttagt
    19201 tgttgcaata cttctatttt cttaggacct atacctttta attgtaaaag agaatatgga
    19261 ctttctatta agtttacttt agccaaaata aatcacctat gttttatttg attttacgta
    19321 tcatttagta tgttatttat tttatcatga ctaagttgaa catgacattg ttagcaactc
    19381 gagaatgata cttttaaccc gcaataacac taaaatagca aaataaaaaa ctaccaactg
    19441 atgatttatc attggtagtt ttttatttta aatttttatt ctactgaaaa gaaatattga
    19501 taaattggtt gtccaccttc atgaacttcc acttctacat ctggatattg ctcttcgatc
    19561 cagtttatca tgttatctgt aactgcttgc tctgcatctt gaccaataat cacagtcaat
    19621 atttcactat cttctgctaa catctcattt aacaactcag taaccgttgt taattgatca
    19681 ctttggctgc ttacaatctt atcttcaatc aagcccataa acgcgtcttt tttaatctca
    19741 acgccatcaa ttttcgtatc acgaacagcg tacgttaatg aaccagattt aacgttattt
    19801 actgaatcag ccatttgcgc tttattttct tcaagtgttg cgtccacatc atattggaat
    19861 agtgcgctta taccttgagg aatagatttc gttggaataa caactgcttc tgcatcaaca
    19921 atactcgctg cttgttcact tgccattaaa atatttttat tattcggtaa aataattgca
    19981 cgtttacatt ttgattgttc aatgacttta acgatatctt ctgtagaagg attcatcgtt
    20041 tgtccaccac taatgatatg tgtggcaccc attgatttga atatctctga aataccttca
    20101 cccatagaaa tagtaataat tgctgtttca accgtttcca ttttcggttt agctgtgtgc
    20161 tgttcttttc gaatcacttc acggtgctgt tctctcatat tttcaacctt aagtttaatt
    20221 aattcaccat attgttgacc ataattaaac actttacctg ggtattcggt atgcacgtga
    20281 actttcacaa tttcttcatc attaatgact aataaagaat caccaaattg actcatatct
    20341 tgcctgaatt cttgttcatc aaaggctttt ttattctttc caaaacgaac catcatttca
    20401 gtacaatagc cataaataat atcttcagta ttaattacac catggaaatc atgttcatca
    20461 tgtacaaatt catctttatc tatctttgca actttggctt caactttttc acctttaagc
    20521 gcttttaaga atccttcgta aacgcataac aaacctttac cgccactatc aacaacacca
    20581 acttctttaa gtacagctaa taagtttggt gtgttttcaa gtgattcatt ggcttttaca
    20641 ataatgtact ccattaattc tatacaatct tcagtattat ttgctttttc tattgcagct
    20701 tgcgcagcat cttttgcaac tgtaagtatt gtaccttcaa ctggtttcat aacagcttta
    20761 tatgccgttt caacaccagc ttgaaaactt tcagctaaca attttgaatt aatttcagat
    20821 tcactttcaa tatttttaca aaatcctctg aataattgtg acaagatgac accagagtta
    20881 cctcttgcac ccattagtaa acctttcgag aatgttttac ctaattcgcc gatatttttc
    20941 gacaaattat tctctacttc ttcgcgacct gaagtcatag taagattcat atttgttcct
    21001 gtatcaccat ctggcactgg atacacattc aaagaatcta ccaaatctgc attgttagat
    21061 aaattttgtg ccccttgtat aatcatatcg gcaaataatt taccattaat tttgctaatc
    21121 atttcaagtt gtcctcctaa gctttcttgc ctgtattatt cacacgtaca ccttgtacat
    21181 atatattgat tgaatttact gatacattaa gtgatttttc caaagtatat ttcactgttg
    21241 attgtacatt attggcaact tcagatattt tcacaccgta acttacaata atgtacatat
    21301 ctatatccac tacgccatta ttttcagtta ctttgatgcc tttagcataa ttttcatgtc
    21361 ctagtatttc cgcaatacca tctctaactt gttgtctaga tgccatacct acaataccat
    21421 aacattcaac ggcctttcca cctacaaccg aagcaatcac ttcgtttgaa atatcaattt
    21481 taccgtaatc atttgaaatc tctaatgtca tatctttgtc cctcctaaat ttcaaagttt
    21541 attattcgtt ttccctaatc acttttaaaa ttttaccact tagtgtagtc ataaacgctt
    21601 taagtttcat tgattatttt tataaaatat caatggtaca acttatcaga atgacttacg
    21661 cttaaatcac ctattatatt ttgagtaaac tgaatttaca ctcatgacga ttatctatag
    21721 gcgttttctt ttaatagatg tagtatcatt aaataaaaat tttaaatgtt tcattcactt
    21781 atttctctaa aatatcatac tacataactt cacagtctac aattatatca caaaactcag
    21841 tatttttgac ttttatgtga aaattattca ttgtttttca aatgaaacta tgctatctta
    21901 attaagtata taagtagtta tgtgtattta tatttattaa aggaggtact catatgggta
    21961 aacaatgttt cgtaacaggt cgtaaagctt cgactggtaa cagacgttca cacgctttaa
    22021 actctactaa acgtagatgg aacgctaacc ttcaaaaagt tagaatccta gttgacggta
    22081 aacctaaaaa agtttgggtt tctgcacgtg ctttaaaatc tggtaaagta actagagttt
    22141 aataaatata ttgcacataa aaaatgtgac cttatgtgga ggacacattt acttaagaac
    22201 taagagctca acctcttagt tctttttttg ttaccaaaca atacataatg gttcatagtg
    22261 aaattcgaac tatcaatata atttggttaa tacgtttaat aatggttctc tgtcaaattg
    22321 gacttatggg ttcaatatcg attttggcac ataaatcaat gtactaggac ctacaatttt
    22381 cctttcaata ataaatcacg ccataatctt cattaattac agcgtgattc atct
//
LOCUS       DAMZKC010000026        22060 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.026, whole genome shotgun sequence.
ACCESSION   DAMZKC010000026 DAMZKC010000000
VERSION     DAMZKC010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 22060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22060
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.026"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(709..2091)
                     /locus_tag="POB55_000760"
     CDS             complement(709..2091)
                     /locus_tag="POB55_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDI6499744.1"
                     /translation="MKNTLYHQLYEKLKKQIIEGQFKEGDKFYSKRQLSKHLSISQTT
                     VEHAYQLLLDEGYIYSRPRSGYFVSEIESLTILNNQPIPSLFDDDSYKPKASDEAYDY
                     AFNLDEIDTKHFPIELFRKYSKDLYDTNHLNQLRRGHFQGELHLRFQLAFYLFTNRGV
                     ICDPNQIIIGSSTEQLVNQLVDLLYTSTFIIEKPSYPPIKNILDKKQVEYEQIEVEDN
                     GINVDEVIKSQKNIVYITPSHQFPTGYVMDLKKRTQLIQWAQEKEERFIIEDDYDSEF
                     RYFGKPIPAIQGLYSRGEKVIYISTFSKSIFPSCRVAYMVLPYSIMKKYHSQNHIEGN
                     TVPVHMQNLIATFISSGGFERHLNKMRRIYRRKLTYILKRLKPYKEQLDIQGAETGMH
                     FTITVKNGLTLQECLDRANKVKLKLQVYNFDDDQDYKKTPKFILGFGGIDDDALKPHV
                     DALIKSLIIK"
     gene            2195..3082
                     /gene="pdxS"
                     /locus_tag="POB55_000761"
     CDS             2195..3082
                     /gene="pdxS"
                     /locus_tag="POB55_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011303857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HDI6499745.1"
                     /translation="MSKIIGSDRVKRGMAEMQKGGVIMDVVNAEQARIAEEAGAVAVM
                     ALERVPSDIRAAGGVARMANPKIVEEVMNAVSIPVMAKARIGHITEARVLEAMGVDYI
                     DESEVLTPADEEYHLRKDQFTVPFVCGCRNLGEAARRIGEGAAMLRTKGEPGTGNIVE
                     AVRHMRQVNSEVSRLTVMNDDEIMTFAKDIGAPYEILKQIKDNGRLPVVNFAAGGVAT
                     PQDAALMMELGADGVFVGSGIFKSEDPEKFAKAIVQATTHYQDYELIGRLASELGTAM
                     KGLDINQLSLEERMQERGW"
     gene            3086..3646
                     /gene="pdxT"
                     /locus_tag="POB55_000762"
     CDS             3086..3646
                     /gene="pdxT"
                     /locus_tag="POB55_000762"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HDI6499746.1"
                     /translation="MKIGVLALQGAVREHIRHIELSGHEGIAVKKVEQLEEIEGLILP
                     GGESTTLRRLMNLYGFKEALQNSTLPMFGTCAGLIVLAQDIVGEEGYLNKLNITVQRN
                     SFGRQVDSFETELDIKGIATDIEGVFIRAPHIEKVGQGVDILCKVNEKIVAVQQGKYL
                     GVSFHPELTDDYRVTDYFINHIVKKA"
     gene            complement(3852..5066)
                     /locus_tag="POB55_000763"
     CDS             complement(3852..5066)
                     /locus_tag="POB55_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDI6499747.1"
                     /translation="MHILIGIIGIIFFLALAFLFSSDRKNIRWKYVGILLAIQLIFAF
                     ILLKTTMGIKVIGGISNGFNYLLLKAADGVNFVFGGIQYIDPKQPPFFFSVLLPIVFI
                     SAIIGILQYTKILPLIINVLGFLISKINGMGRLESYNAVAAAILGQSEVFISIKKQLP
                     YIPKQRLYTLTASAMSTVSASIIGAYFTLIEPKYVVTAVVLNLFGGFIIASIINPYKV
                     NEEDDKLLVEETEERQQSFFEMLGEYILDGFKVAVIVGAMLIGYIAIIALLNGIVSNI
                     FSTVSGGAISWDFQTLIGFVFAPFAFLVGVPWQDAVQAGSVMATKLLSNEFVAMQALG
                     KLSDLSEHAKGVTSVFLVSFANFSSIGIISGAIKSLNDKKGDTVARFGLKLLFGATLV
                     SFISAAIAGFFI"
     gene            5224..5685
                     /locus_tag="POB55_000764"
     CDS             5224..5685
                     /locus_tag="POB55_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HDI6499748.1"
                     /translation="MHNMSDIIEQYIKRLFEESNEDVVEIQRANIAQRFDCVPSQLNY
                     VIKTRFTNEHGYEIESKRGGGGYIRITKIENKDATGYINHLLQLIGPSISQQQAYYII
                     DGLLDKMLINEREAKMIQAVIDRETLSMDMVSRDIIRANILKRLLPVINYY"
     gene            5704..6270
                     /locus_tag="POB55_000765"
     CDS             5704..6270
                     /locus_tag="POB55_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HDI6499749.1"
                     /translation="MLCENCQLNEAELKVKVTSKNKTEEKMVCQTCAEGHHPWNQANE
                     QPEYQEHQDNFEEAFVVKQILQHLATKHGINFQEVAFKEEKRCPSCHMTLKDIAHVGK
                     FGCANCYATFKDDIIDIVRRVQGGQFEHVGKTPHSSHKKIALKRKIEEKNEYLKKLIE
                     IQDFEEAAIVRDEIKALKAESEVQHDDA"
     gene            6260..7267
                     /locus_tag="POB55_000766"
     CDS             6260..7267
                     /locus_tag="POB55_000766"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HDI6499750.1"
                     /translation="MTHNIHDNISQWMKSNEETPIVMSSRIRLARNLENHVHPLMYAT
                     ENDGFRVINEVQDALPNFELMRLDQMDQQSKMKMVAKHLISPELIKQPAAAVLVNDDE
                     SLSVMINEEDHIRIQAMGTDTTLQALYNQASSIDDELDRSLDISYDEQLGYLTTCPTN
                     IGTGMRASVMLHLPGLSIMKRMTRIAQTINRFGYTIRGIYGEGSQVYGHTYQVSNQLT
                     LGKSELEIIETLTEVVNQIIHEEKQIRQKLDTYNQLETQDRVFRSLGILQNCRMITME
                     EASYRLSEVKLGIDLNYIELQNFKFNELMVAIQSPFLLDEEDDKSVKEKRADILREHI
                     K"
     gene            7281..9737
                     /locus_tag="POB55_000767"
     CDS             7281..9737
                     /locus_tag="POB55_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HDI6499751.1"
                     /translation="MLFGRLTERAQRVLAHAQEEAIRLNHSNIGTEHLLLGLMKEPEG
                     IAAKVLESFNITEDKVIEEVEKLIGHGQDHVGTLHYTPRAKKVIELSMDEARKLHHNF
                     VGTEHILLGLIRENEGVAARVFANLDLNITKARAQVVKALGNPEMSNKNAQASKSNNT
                     PTLDSLARDLTVIAKDGTLDPVIGRDKEITRVIEVLSRRTKNNPVLIGEPGVGKTAIA
                     EGLAQAIVNNEVPETLKDKRVMSLDMGTVVAGTKYRGEFEERLKKVMEEIQQAGNVIL
                     FIDELHTLVGAGGAEGAIDASNILKPALARGELQCIGATTLDEYRKNIEKDAALERRF
                     QPVQVDEPSVVDTVAILKGLRDRYEAHHRINISDEAIEAAVKLSNRYVSDRFLPDKAI
                     DLIDEASSKVRLKSHTTPNNLKEIEQEIEKVKNEKDAAVHAQEFENAANLRDKQTKLE
                     KQYEEAKNEWKNAQNGMSTSLSEEDIAEVIAGWTGIPLTKINETESEKLLSLEDTLHE
                     RVIGQKDAVNSISKAVRRARAGLKDPKRPIGSFIFLGPTGVGKTELARALAESMFGDD
                     DAMIRVDMSEFMEKHAVSRLVGAPPGYVGHDDGGQLTEKVRRKPYSVILFDEIEKAHP
                     DVFNILLQVLDDGHLTDTKGRTVDFRNTIIIMTSNVGAQELQDQRFAGFGGSSDGQDY
                     ETIRKTMLKELKNSFRPEFLNRVDDIIVFHKLTKEELKEIVTMMVNKLTNRLSEQNIN
                     IIVTDKAKDKIAEEGYDPEYGARPLIRAIQKTIEDNLSELILDGNQIEGKKVTVDHDG
                     KEFKYDIAEQTSETKTPSQA"
     gene            10221..11585
                     /gene="radA"
                     /locus_tag="POB55_000768"
     CDS             10221..11585
                     /gene="radA"
                     /locus_tag="POB55_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HDI6499752.1"
                     /translation="MAKKKVIFECMACGYQSPKWMGKCPNCGAWNQMEEIVEKAANPK
                     HGVKTKELAGKVQKLNSIKHETTPRVLTDSAEFNRVLGGGIVSGSLVLIGGDPGIGKS
                     TLLLQICASLSQKKKVLYITGEESLSQTKLRAERLDEDSSELQVLAETDLEVIYQTVK
                     EEQPDLLVVDSIQTIYHPEISSAPGSVSQVRESTQSLMNIAKQMNIATFIVGHVTKEG
                     QIAGPRLLEHMVDTVLYFEGDEHHAYRILRAVKNRFGSTNEMGIFEMKQSGLKGVNNP
                     SEMFLEERSTNVPGSTIVATMEGTRPLLIEVQALVTPTTFNNPRRMATGIDHNRLSLL
                     MAVLEKKENYLLQQQDAYIKVAGGVKLTEPAVDLSVIVATASSFKDKAVDGLDCYIGE
                     VGLTGEVRRVSRIEQRVQEAAKLGFKRVIIPKNNIGGWTYPEGIQVIGVTTVHEALSF
                     ALHS"
     gene            11610..12683
                     /locus_tag="POB55_000769"
     CDS             11610..12683
                     /locus_tag="POB55_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017328539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HDI6499753.1"
                     /translation="MNIVKLMVIIIYLIIGSALGIIIIPEIANDLGLQNSSFLKNHYV
                     DGIIGSIFMFLIFGVFIRRVTNAIKGLEHFIMRRSAVEILFATIGLIIGLLISVMVSF
                     ILESIGNSIFNHFIPVIITILLCYFGFQFGLKKRDEMLMFLPENIARSMSQHTKSATP
                     KIIDTSAIIDGRILEVIRCGFIDGNILIPQGVINELQIVADSNDSVKREKGKRGLDIL
                     NELYDLDYPTKVIHPTKTHSDIDTMLLKLAKQYHASIITTDFNLNKVCHVHGIKALNV
                     NDLSEAIKPNVHQGDQLHILLTKMGKEPGQAVGYLDDGTMVVVDNAKNLIGSHVNLEV
                     VSLLQTSSGRIVFAKKIEDTVSL"
     gene            13240..14694
                     /locus_tag="POB55_000770"
     CDS             13240..14694
                     /locus_tag="POB55_000770"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HDI6499754.1"
                     /translation="MSDRIRVRYAPSPTGYLHIGNARTALFNYLYAKHYNGDFVIRIE
                     DTDKKRNLEDGETSQFDNLKWLGLDWDESVDKDNGYGPYRQSERQHIYQPLIDQLLAE
                     DKAYKCYMTEEELEAEREAQIARGEMPRYGGQHAHLTEEQRQQFEAEGRQPSIRFRVP
                     QNQTYSFDDMVKGNISFDSNGIGDWVIVKKDGIPTYNFAVAIDDHYMQISDVIRGDDH
                     ISNTPKQIMIYEAFGWEPPRFGHMSLIVNEERKKLSKRDGQILQFIEQYRDLGYLPEA
                     LFNFIALLGWSPEGEEEIFSKEEFIKIFDEKRLSKSPAFFDKQKLAWVNNQYMKQKDT
                     ETVFQLALPHLIKANLIPEVPSEEDLSWGRKLIALYQKEMSYAGEIVPLSEMFFKEMP
                     ALGEEEQQVINGEQVPELMTHLFSKLEALEPFEAAEIKKTIKEVQKETGIKGKQLFMP
                     IRVAVTGQMHGPELPNTIEVLGKEKVLNRLKQYK"
     gene            15117..15764
                     /gene="cysE"
                     /locus_tag="POB55_000771"
     CDS             15117..15764
                     /gene="cysE"
                     /locus_tag="POB55_000771"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HDI6499755.1"
                     /translation="MILLKRMRDDIKMVFEQDPAARSTLEVITTYAGLHAVWSHLIAH
                     KLYNQKKYVAARAISQISRFFTGIEIHPGAKIGKRLFIDHGMGVVIGETCTIGDNVTI
                     YQGVTLGGTGKERGKRHPDIGDNVLIAAGAKVLGNIKINSNVNIGANSVVLQSVPSYS
                     TVVGIPGHIVKQDGVRVGKTFDHRHLPDPIYEQIKHLERQLEKTRNGEIQDDYII"
     gene            15748..17148
                     /locus_tag="POB55_000772"
     CDS             15748..17148
                     /locus_tag="POB55_000772"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000631968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HDI6499756.1"
                     /translation="MITLYNTLTRQKEVFKPIEPGKVKMYVCGPTVYNYIHIGNARPA
                     INYDVVRRYFEYQGYNVEYVSNFTDVDDKLIKRSQELNQSVPEIAEKYIAAFHEDVGV
                     LNVRKATSNPRVMDHMDDIIQFIKDLVDRGYAYESGGDVYFRTRKFEGYGKLSHQSID
                     DLKVGARIDAGEHKEDALDFTLWKKAKPGEISWDSPFGEGRPGWHIECSVMAFHELGP
                     TIDIHAGGSDLQFPHHENEIAQSEAHNHAPFANYWMHNGFINIDNEKMSKSLGNFILV
                     HDIIKEVDPDVLRFFMISVHYRSPINYNLELVESARSGLERIRNSYQLIEERAQIATN
                     IENQQTYIDQIDAILNRFETVMNDDFNTANAITAWYDLAKLANKYVLENTTSTEVIDK
                     FKAVYQIFSDVLGVPLKSKNADELLDEDVEKLIEERNEARKNKDFARADEIRDMLKSQ
                     NIILEDTPQGVRFKRG"
     gene            17141..17545
                     /locus_tag="POB55_000773"
     CDS             17141..17545
                     /locus_tag="POB55_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HDI6499757.1"
                     /translation="MDNQQDNHIKLLNPLTLAYMGDAVLDQYVRTYIVLKLKSKPNKL
                     HQMSKKYVSAKSQAQTLEYLMEQEWFTDEEMDILKRGRNAKSHTKAKNTDVQTYRKSS
                     AIEAVIGFLYLEKREERLEALLNKIITIVNER"
     gene            17553..18299
                     /gene="rlmB"
                     /locus_tag="POB55_000774"
     CDS             17553..18299
                     /gene="rlmB"
                     /locus_tag="POB55_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HDI6499758.1"
                     /translation="MEDTVIVGRHAVREAIITGHPINKILIQEGIKKQQINEILKNAK
                     DQKIIVQTVPKSKLDFLANAPHQGVAALIAPYEYADFDQFLKQQKEKEGLSTVLILDG
                     LEDPHNLGSILRTADATGVDGVIIPKRRSVTLTQTVAKASTGAIEHVPVIRVTNLAKT
                     IDELKDNGFWVAGTEANNATDYRNLEADMSLAIVIGSEGQGMSRLVSDKCDFYIKIPM
                     VGHVNSLNASVAASLMMYEVFRKRHDVGEI"
     gene            18299..18823
                     /locus_tag="POB55_000775"
     CDS             18299..18823
                     /locus_tag="POB55_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HDI6499759.1"
                     /translation="MKERYLIIDGYNMIGQSPTLSAIAKENLEEARMQLIDAIANYNA
                     VISDEIICVFDAYDQSGVEREYMYHGVKTIFTKEKETADSFIERYVYELYDKHTKHIT
                     VVTSDMSEQHAIFGSGAYRISSREMWRDLKENEIDVSKSLDDISENKPRTRIPLSSEI
                     LAEFEKIRRGHHKK"
     gene            18904..19473
                     /locus_tag="POB55_000776"
     CDS             18904..19473
                     /locus_tag="POB55_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HDI6499760.1"
                     /translation="MKYDLTTQDSTIKRNNAINDKDFEKLVMDLQPLIIRRIKTFGFN
                     HYDLEDLYQEILIRMYRSVQTFDFSGEQPFTNYVQCLITSVKYDYLRKYLATNKRMDN
                     LINEYRVTYPCAIKRYDVENNYLNKLAIKELIRQFKYLSVFEKDVMYLMCEQYKPREI
                     AQLMHVKEKVIYNAIQRCKNKIKRYFKMI"
     gene            19588..19731
                     /gene="rpmG"
                     /locus_tag="POB55_000777"
     CDS             19588..19731
                     /gene="rpmG"
                     /locus_tag="POB55_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002451211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDI6499761.1"
                     /translation="MRKIPLNCEACGNRNYNVPKQEGSATRLTLKKYCPKCNAHTIHK
                     ESK"
     gene            19787..19969
                     /gene="secE"
                     /locus_tag="POB55_000778"
     CDS             19787..19969
                     /gene="secE"
                     /locus_tag="POB55_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HDI6499762.1"
                     /translation="MAKKESFFKGVKSEMEKTSWPTKEELFKYTVIVVSTVIFFLVFF
                     YALDLGITALKNLLFG"
     gene            19982..20530
                     /gene="nusG"
                     /locus_tag="POB55_000779"
     CDS             19982..20530
                     /gene="nusG"
                     /locus_tag="POB55_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HDI6499763.1"
                     /translation="MSEEVGAKRWYAVHTYSGYENKVKKNLEKRVESMNMTEQIFRVV
                     IPEEEETQVKDGKAKTTVKKTFPGYVLVELIMTDESWYVVRNTPGVTGFVGSAGAGSK
                     PNPLLPEEVRFILKQMGLKEKTIDVELEVGEQVRIKSGPFANQVGEVQEIETDKFKLT
                     VLVDMFGRETPVEVEFDQIEKL"
     gene            20711..21133
                     /gene="rplK"
                     /locus_tag="POB55_000780"
     CDS             20711..21133
                     /gene="rplK"
                     /locus_tag="POB55_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012664221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HDI6499764.1"
                     /translation="MAKKVDKVVKLQIPAGKANPAPPVGPALGQAGVNIMGFCKEFNA
                     RTQDQAGLIIPVEISVYEDRSFTFITKTPPAPVLLKKAAGIEKGSGEPNKTKVATVTK
                     DQVREIANSKMQDLNAADEEAAMRIIEGTARSMGIVVE"
     gene            21341..22033
                     /gene="rplA"
                     /locus_tag="POB55_000781"
     CDS             21341..22033
                     /gene="rplA"
                     /locus_tag="POB55_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HDI6499765.1"
                     /translation="MAKKGKKYQEAASKVDRTQHYSVEEAIKLAKETSIANFDASVEV
                     AFRLGIDTRKNDQQIRGAVVLPNGTGKSQSVLVFAKGDKIAEAEAAGADYVGEAEYVQ
                     KIQQGWFDFDVVVATPDMMGEVGKLGRVLGPKGLMPNPKTGTVTMDVKKAVEEIKAGK
                     VEYRAEKAGIVHASIGKVSFTDEQLIENFNTLQDVLAKAKPSSAKGTYFKSVAVTTTM
                     GPGVKIDTASFK"
BASE COUNT         8152 a         3031 c         4242 g         6635 t
ORIGIN      
        1 aaaatggatg cgatgagccg cattgagacc gcaaggtctc tttttttatg tctaaaacgt
       61 caaaataaaa agcaaacaca aagaaaaatg gcttggcgaa gtgaaaacgt ttgaatctga
      121 cgaaacgaga aaagaacgca acgagtttag tagagctaaa tgagtaagtg agagccgtag
      181 gagaggaaag aagcaagcga ttgtcacaag tcaagaaagg ttcttagcga cgatggtagc
      241 caacttacgt tccgctagag tagaacgttg ccaggcaaaa atggatgcga tgagccgcat
      301 tgagaccgtg aggtctcttt ttttatgtct aaaacgtcaa aataaaagtt aacacaaaga
      361 aaatggcttg acgaagtgaa aacgtttgaa tctgacgaaa cgagaaaaga gcgcaacgag
      421 tttagtagag ctaaatgagt aagcgagagc cgaagaaaat gataatttaa taatgtacac
      481 tttcgattgt ctaagtatgt ataactttaa ttttgtgttt atataaattt aaaatgatat
      541 catcgaaaac aaaatattgt ataaatagag aagagcagta agacggtatc taattgaaaa
      601 tgatcttact gctcttttat atactttatt gaaatacaaa atggaaatta attattatac
      661 aatagacaag ctattgcata agtaacacta acttttatca aagaagtgtt actttataat
      721 taatgatttt attagagcgt ctacatgcgg ttttaaagca tcatcgtcta taccgccaaa
      781 gcctaatata aatttagggg ttttcttata gtcttgatca tcatcaaaat tataaacttg
      841 taattttaac tttactttgt ttgctctatc aagacactct tgtaatgtta atccattttt
      901 tactgtaatt gtaaaatgca tacccgtttc agcaccttga atatcaagct gctctttgta
      961 aggtttcaat ctttttaaaa tataggttag ttttctacga taaattcgtc tcattttatt
     1021 taaatgcctt tcaaaaccac cggaagatat aaacgttgca ataaggtttt gcatatgaac
     1081 aggtacagtg ttgccttcaa tgtgattttg agaatgatat tttttcatta tagaataggg
     1141 taacaccata tatgcaactc gacagctagg aaaaatagac tttgaaaatg tactgatata
     1201 aatcactttt tctcctcttg aatatagacc ttgaattgct ggaatgggtt tgccgaaata
     1261 tctaaactcg gaatcataat catcttctat aataaatcgt tcttcttttt cttgagccca
     1321 ttgtattaat tgagttcgct tttttaagtc catcacatat ccagttggaa attgatggga
     1381 aggcgttata tatactatat ttttttgtga tttaataact tcatctacgt ttattccatt
     1441 atcttcaact tcaatttgtt catattcaac ttgtttttta tctaaaatat ttttgattgg
     1501 tggataacta ggtttttcga taataaatgt tgaagtataa agtaaatcga ctaattgatt
     1561 tactaattgt tcggtagatg agccaattat aatttgatta ggatcacaaa ttacaccacg
     1621 attagtaaat aaataaaatg ccagttgaaa tcttaaatgt aattctcctt gaaaatgtcc
     1681 tctacgtaat tgatttaaat gatttgtatc ataaagatct ttggaatact ttctgaaaag
     1741 ttctataggg aaatgtttcg tatctatttc atccaaatta aaagcataat cataagcttc
     1801 atcactcgct tttggtttat atgaatcatc atcaaaaaga gaggggatag gttgattgtt
     1861 taaaattgtt aaagattcaa tttcggacac aaaatatcca gagcgaggtc ttgaataaat
     1921 gtaaccttcg tctaatagaa gttgatatgc atgctctacg gttgtttggc tgatagataa
     1981 atgtttgctt aattgtcttt tagaataaaa tttatcgcct tctttaaatt gaccttcaat
     2041 tatttgtttt tttaattttt cataaagttg atggtataaa gtgtttttca attttataac
     2101 tgacctccta aatttatctt attttgtacc tttttaaata tcagtttata cattacaatg
     2161 tatttaatca acttgaaaag gggttttatg tataatgagt aaaattattg gatcagacag
     2221 agtcaaaaga ggtatggctg aaatgcaaaa aggcggcgtt attatggatg tcgttaatgc
     2281 tgagcaagca agaattgcag aagaagcagg cgcggtagca gttatggcat tagaacgagt
     2341 accttctgat attagagctg ctggtggcgt tgcacgtatg gcaaacccta aaattgtaga
     2401 agaagtaatg aatgctgttt ctattccagt catggctaaa gcacgtattg gtcatatcac
     2461 tgaagcaaga gtattagagg cgatgggtgt tgactatatt gatgaatcag aagtgttaac
     2521 accagcagat gaggaatatc acttaagaaa agatcaattt acagtaccat ttgtatgtgg
     2581 atgtcgtaat ttaggtgaag ctgcgcgtag aattggtgaa ggtgctgcta tgttacgtac
     2641 taaaggtgaa ccaggtacag gtaatattgt tgaagctgta agacatatga gacaagttaa
     2701 ttcagaagtt agtcgattga ctgtaatgaa tgatgatgag attatgactt ttgcgaaaga
     2761 tatcggtgcg ccttatgaaa ttttaaaaca aattaaagac aatggtcgtt taccggtagt
     2821 taactttgca gctggtggcg ttgcgactcc tcaagatgct gctttaatga tggaattagg
     2881 tgccgacggt gtattcgttg gatcaggtat ttttaaatca gaagatccag aaaaatttgc
     2941 taaagcaatt gttcaagcaa caacacatta ccaagactat gagctaattg gaagattagc
     3001 aagtgaactt ggcactgcta tgaaaggttt agatatcaat caattatcat tagaagaacg
     3061 tatgcaagag cgtggttggt aagatatgaa aataggtgta ttagcattac aaggtgcagt
     3121 acgtgaacat attagacata ttgaattaag tggtcatgaa ggtattgcag ttaaaaaagt
     3181 tgaacaatta gaagaaatcg agggcttaat attacctggt ggcgagtcta caacgttacg
     3241 tcgattaatg aatttatatg gatttaaaga ggctttacaa aattcaactt tacctatgtt
     3301 tggtacatgc gcaggattaa tagttctagc gcaagatata gttggtgaag aaggatacct
     3361 taacaagttg aatattactg tacaacgaaa ctcattcggt agacaagttg acagctttga
     3421 aacagaatta gatattaaag gtatcgctac agatattgaa ggtgtcttta taagagcgcc
     3481 acatattgaa aaagtaggcc aaggcgtaga tatcctatgt aaggttaatg agaaaattgt
     3541 agccgtccag caaggtaaat atttaggcgt atcattccat cctgaattaa cagatgacta
     3601 tagagtaact gattacttta ttaatcatat tgtaaaaaaa gcatagctta atgtatgcta
     3661 aatcaacgaa ttattgatat ttatagattt gttgagaaga caatatctcc ttcaaactta
     3721 gctttggagg agctattttt ttatgtcaaa attaaaaatg ataaaaaata aagatataca
     3781 taaaaaaacc cttcaaagag actgagaata gtcaaaattt tgaaggggtt aattcgatgt
     3841 tgatgtattt gttaaataaa gaatccagcg attgcagctg aaatgaaaga tactagtgtt
     3901 gcaccgaata atagtttcaa accaaagcgg gcaactgtat ctcctttttt gtcattaagt
     3961 gatttaatcg cacctgaaat aataccaata gagctaaagt tagcaaatga tactaagaat
     4021 acagatgtaa caccttttgc gtgttcagat aaatcactaa gtttaccaag tgcttgcatt
     4081 gctacaaatt cgttagataa tagttttgtc gccataactg aaccggcttg aactgcatct
     4141 tgccatggca caccgactaa gaatgcaaat ggtgcaaaga caaaaccaat taatgtttgg
     4201 aaatcccaag aaatagcgcc acctgaaact gtactaaaga tattgcttac aattccattt
     4261 aatagagcga taatagcaat gtatccgatt aacattgcgc ctacaatgac agctacttta
     4321 aatccatcta aaatatattc tcctagcatt tcgaagaatg attgttgtct ttcttcagtt
     4381 tcttcaacta ataatttgtc atcttcttca ttaactttat aagggttaat aattgaagcg
     4441 atgatgaaac caccaaataa gtttaagaca acagccgtta caacatattt aggttcaatt
     4501 aaggtaaagt atgcaccgat aattgaagca gaaacagtcg acattgctga agctgttaat
     4561 gtgtataaac gttgcttagg tatgtatggt aattgttttt taattgaaat aaatacttca
     4621 gattgtccca aaattgctgc agcaactgca ttgtatgatt ctaaacgtcc cataccatta
     4681 attttagaaa ttaagaatcc taaaacatta atgattaaag gtaaaatctt tgtgtattga
     4741 aggataccga taatcgctga aataaatacg ataggtaata atacactgaa gaagaatggt
     4801 ggttgcttag gatcgatata ttgaatacca ccgaatacaa agttaacacc atctgctgct
     4861 tttaataata agtagttaaa accgtttgaa ataccaccaa taaccttgat tcccattgta
     4921 gttttaagca agataaatgc aaagataagc tgaattgcaa gtaaaattcc tacatatttc
     4981 cagcgaatat ttttcctgtc tgagctaaat agaaacgcaa gtgctaaaaa gaagataatt
     5041 ccgataatcc caattagaat atgcatatat ttctcattcc tttagttttt tctacaatct
     5101 atcatacaat aaaatggaag ggctaacatc ataaattttt gaaaatataa aaacaaatta
     5161 attgaaaaag gtcaaaatag gtcatataat atagtcaaag aaggtcaaaa aggggtgata
     5221 tacatgcaca atatgtctga catcatagaa caatacatca aacgtttatt tgaagagtcg
     5281 aatgaagatg tcgttgaaat tcagagagcg aatatcgcac agcgttttga ttgtgtacca
     5341 tcacaattaa attatgtaat caaaacacga ttcactaatg aacatggtta tgaaatcgaa
     5401 agtaaacgtg gtggtggtgg ttacatccga atcactaaaa ttgaaaataa agatgcaaca
     5461 ggttatatta atcatttgct tcagctgatt ggaccttcta tttctcaaca acaagcttat
     5521 tatattattg atgggctttt agataaaatg ttaataaatg aacgtgaagc taaaatgatt
     5581 caagcagtta ttgatagaga aacgctatca atggatatgg tttctagaga tattattaga
     5641 gcaaatattt taaaacgttt gttaccagtt ataaattatt actaaatgaa atgaggtgtt
     5701 gaagtgcttt gtgaaaattg tcaacttaat gaagcggaat taaaagttaa agttacaagt
     5761 aaaaataaaa cagaagaaaa aatggtgtgt caaacttgtg ctgaggggca ccatccgtgg
     5821 aatcaagcta atgaacaacc tgaatatcaa gaacatcaag ataatttcga agaagcattt
     5881 gttgttaagc aaattttaca acatttagct acgaaacatg gcattaattt tcaagaagta
     5941 gcgtttaaag aagaaaaacg ttgcccatca tgtcatatga ctttgaaaga tattgcacat
     6001 gttggtaaat ttgggtgtgc taattgttat gcaacattta aagatgacat cattgatatc
     6061 gtccgcagag ttcaaggtgg acaatttgag cacgttggaa agacaccaca ttcttcacat
     6121 aaaaagatag ctttaaagcg aaaaatcgaa gaaaagaatg aatatttgaa aaaacttatt
     6181 gaaatccaag attttgagga agcagccatt gttagagatg aaattaaagc actaaaagct
     6241 gagagtgagg tgcaacatga tgacgcataa tattcatgat aatatcagcc aatggatgaa
     6301 aagtaatgaa gaaacaccaa ttgttatgtc ttctagaatt cggttagcgc gtaatttaga
     6361 aaatcatgtg catccactaa tgtatgctac tgaaaatgat ggatttagag ttataaatga
     6421 ggtacaagat gccttgccaa attttgaatt aatgcgtctt gatcaaatgg atcaacaaag
     6481 taaaatgaaa atggttgcaa agcatttgat tagtcctgaa ctaataaaac aaccagcagc
     6541 cgcagtatta gtgaatgatg atgaatcttt aagtgtcatg ataaatgaag aggaccatat
     6601 tcgtattcaa gctatgggaa ctgacacgac attacaggct ttatataatc aagcttcatc
     6661 aattgatgat gaattagatc gaagccttga tataagttat gatgaacaac ttggttattt
     6721 aactacatgt cctaccaata taggtactgg tatgagagca agcgtgatgc tacatttacc
     6781 aggtctatct attatgaaaa gaatgacacg gattgctcaa accattaatc gttttggata
     6841 tacaatcaga ggtatttacg gtgaaggttc gcaagtttat ggacatactt atcaagtatc
     6901 caaccaactt acacttggta aatctgagtt agaaatcata gaaacattaa cagaagttgt
     6961 taatcaaatc attcatgaag aaaaacaaat acgacaaaag ttagacactt ataatcaatt
     7021 agaaacacaa gaccgtgttt ttcgctcgct aggtatttta caaaactgta gaatgataac
     7081 tatggaagag gcttcttata gattaagcga agttaaactt ggtatagatt taaattacat
     7141 tgaattacaa aactttaaat ttaatgaatt gatggtagct atacagtcac catttttatt
     7201 agatgaagaa gatgacaaat ctgtaaaaga aaaacgagca gatatactaa gagaacatat
     7261 aaagtaggag gtcattattt atgttatttg gtagattaac tgagcgtgca cagcgcgtat
     7321 tagcacatgc acaagaagaa gcaattcgtt taaatcattc aaatatagga acagaacacc
     7381 tattattggg attaatgaaa gaacctgaag gaattgctgc aaaagtatta gaaagtttta
     7441 atatcactga agataaagta attgaagaag ttgaaaaatt aatcggacat ggtcaagatc
     7501 atgttggtac attgcattat acacctagag ctaaaaaagt tattgaatta tcgatggatg
     7561 aagctagaaa attacatcac aattttgttg gaacggaaca tattttatta ggcttgattc
     7621 gtgaaaatga aggtgttgca gcaagagttt ttgcaaatct agatttaaat attactaaag
     7681 cacgtgcaca agttgtgaaa gctttaggaa accctgaaat gagtaataaa aatgcacaag
     7741 ctagtaagtc aaataatact ccaactttag atagtttagc tcgtgactta acagtcattg
     7801 ccaaagacgg tacattagat cctgttatag gacgtgataa agaaattaca cgtgtaattg
     7861 aagtattaag tagacgtacg aaaaacaatc ctgtacttat tggagagcca ggtgttggta
     7921 aaactgctat tgctgaaggt ttagcgcaag ccatagtgaa taatgaggta ccagagacat
     7981 taaaagataa gcgtgttatg tctttagata tgggaacagt agttgcaggt actaaatatc
     8041 gtggtgaatt tgaagagcgt ctgaaaaagg ttatggaaga aatccaacaa gcaggtaatg
     8101 tcatcctatt tattgatgag ttgcatactt tagttggtgc tggtggtgct gaaggtgcta
     8161 tcgatgcttc gaatattttg aaaccggcat tagcacgtgg tgaattacaa tgtattggtg
     8221 ctactacatt agatgaatat cgcaaaaata ttgaaaaaga cgcggcttta gaacgtcgtt
     8281 tccaacctgt acaagttgat gaaccttcag tagtagatac agttgctatt ttaaaaggat
     8341 taagagatcg ttacgaagca caccatcgta ttaatatttc agacgaagct attgaagcag
     8401 ctgttaaatt aagtaacaga tacgtttcag atcgtttctt accagataaa gcaattgatt
     8461 taattgatga agcaagttct aaagtaagac ttaagagtca tacgacacct aataatttaa
     8521 aagaaattga acaagaaatt gaaaaagtta aaaatgaaaa agatgctgca gtacatgctc
     8581 aagagtttga aaatgctgct aacctgcgtg ataaacaaac aaaacttgaa aagcaatatg
     8641 aagaagctaa aaatgaatgg aagaatgcac aaaatggcat gtcaacttca ttgtcagaag
     8701 aagatattgc tgaagttatt gcaggatgga caggtatccc attaactaaa atcaatgaaa
     8761 cagaatctga aaaacttctt agtctagaag atacattaca tgagagagtt attgggcaaa
     8821 aagatgctgt taattcaatc agtaaagcgg ttagacgtgc ccgtgcaggg ttaaaagatc
     8881 ctaaacgacc aattggtagc tttatcttcc ttggaccaac tggtgttggt aaaactgaat
     8941 tagctagagc tttagctgaa tcaatgtttg gcgatgatga tgcgatgatc cgtgtagaca
     9001 tgagtgaatt tatggaaaaa cacgcagtga gccgattagt tggtgctcct ccaggatatg
     9061 ttggtcatga tgatggtgga caattaactg aaaaagttag acgtaaacca tattctgtaa
     9121 ttttatttga tgaaattgaa aaagctcatc cagatgtatt taatattcta ttacaagttt
     9181 tagatgatgg acatttgaca gatacaaaag gacgtacagt tgatttcaga aatacaatta
     9241 tcataatgac atcaaacgtt ggggcacaag aattacaaga tcaacgattt gctggattcg
     9301 gtggttcaag tgatggacaa gattatgaaa caattcgaaa aacgatgtta aaagaattaa
     9361 aaaattcatt ccgtccagaa tttttaaacc gtgtagatga tatcattgta ttccataaac
     9421 taacaaaaga agaattaaaa gaaattgtaa caatgatggt taataaatta acaaatcgat
     9481 tatctgaaca aaacataaat attattgtta ctgataaagc gaaagacaaa atcgcagaag
     9541 aaggatatga tccagaatat ggtgcaagac cattaattag agcgatacaa aaaactatcg
     9601 aagataattt aagtgaatta atattagatg gtaatcaaat tgaaggtaag aaagttacag
     9661 tagatcatga tggtaaagag tttaaatatg acattgctga acaaacttca gaaactaaaa
     9721 caccatcgca agcataatta taaaacagtc caaaacaaat taaagttttg ggctgttttt
     9781 ttagtagcat tgaactatag aaattcgtga aagtatccat caacgaaaca atctataaaa
     9841 caatcatcaa aggatagtta agaattatat gtaacaagtt aatgagccta cagcgatagc
     9901 ataaaggtat gaatttttat aaaggttttt tgtttgaaga tactagcaac ttacgtcaaa
     9961 ataaaatagg tctatataat attgtgataa atctagagaa aagagtttac taagaaatta
    10021 ttaagatttt atctttgaaa gataccgact atcaacatga tgtaagttta ttttataata
    10081 ttcataaaaa ataaatctgg taaaacagtt tgcgttagta gtcatgttaa aatacaaaca
    10141 aatgtaactc acatttaatt tgtcataatg ggaatgtgcg tttaaaatag atttgttcaa
    10201 aggaaagtgg aggtgcaatt ttggccaaga aaaaagtgat ttttgaatgt atggcttgtg
    10261 gttatcaatc tcctaaatgg atggggaaat gtcctaattg tggcgcttgg aatcaaatgg
    10321 aggaaattgt tgaaaaagca gccaatccta aacatggagt taaaaccaag gaattagcag
    10381 gtaaagtaca aaaattaaat agtattaaac atgaaacaac gccgagagtg ttaacagatt
    10441 cagcagaatt caaccgtgta ttaggtggag gtattgtgag cggatcgtta gtacttattg
    10501 gtggggatcc aggtattggt aagtcaacgt tacttttaca aatttgtgca tcgttatctc
    10561 aaaagaaaaa agtactatat attactggag aagaatcgct tagtcagact aaattacgtg
    10621 cagagcgatt agatgaagat tcaagtgaat tgcaagtatt agctgaaaca gatcttgaag
    10681 ttatttatca aacagtaaaa gaagaacaac ctgatttatt agtagtggat tcgattcaaa
    10741 caatatatca tcctgaaatc agctctgcgc caggttctgt ttcacaagtt cgtgaaagta
    10801 cacaaagttt aatgaatatt gctaaacaaa tgaacattgc aacttttata gtgggtcatg
    10861 taacgaaaga aggtcaaatt gctggcccaa gattgctaga acacatggtt gatactgtgc
    10921 tttattttga aggcgatgaa caccacgcat atcgaatttt gcgagctgtt aaaaaccgtt
    10981 ttggttcaac gaatgaaatg ggaatcttcg aaatgaagca aagtggatta aaaggtgtaa
    11041 ataatccatc tgaaatgttt ttagaagaac gttcaacaaa tgttccaggt tcaacaattg
    11101 ttgcaaccat ggagggaacc agaccacttt taatagaagt tcaagcgctg gtaactccaa
    11161 cgacttttaa caatccgaga cgaatggcaa cagggattga tcataatcga ttaagtttgt
    11221 tgatggctgt tttggaaaag aaagaaaatt atctattaca acaacaagat gcttatatca
    11281 aagtagctgg cggtgtaaag ttaacggagc cagcagttga tttaagtgta attgtagcaa
    11341 ctgcatctag ctttaaagat aaagctgtcg acggattaga ttgctatatt ggagaagttg
    11401 gtttaacggg tgaggtacgt cgtgtatctc ggatagaaca acgcgtgcaa gaggctgcaa
    11461 aactaggttt caaacgtgta attattccta aaaataatat aggcggatgg acatatcctg
    11521 aaggtataca agtaataggt gtaactactg tacatgaagc attgtcattt gctcttcatt
    11581 cataaaacat caagaaagga ggacattgtg tgaatatcgt taaactaatg gttattatta
    11641 tttacttaat tattgggagc gcattaggaa taattattat tcctgaaatt gcaaatgatc
    11701 ttggattaca aaactccagc tttttaaaaa atcactatgt agatggcatt atcggtagta
    11761 tttttatgtt cttaattttt ggtgtattta ttagacgagt tactaacgct ataaaaggtt
    11821 tagaacattt tattatgcgt agaagtgctg ttgaaatact attcgcaaca ataggtttaa
    11881 taatcggatt acttatttct gttatggtgt cgtttatatt agaatcaatt ggtaactcta
    11941 tttttaatca tttcattcct gtcataatta cgatattact atgttatttc ggtttccaat
    12001 ttggccttaa aaaacgagat gaaatgttaa tgtttttacc tgagaatata gcgcgttcca
    12061 tgtcacaaca tactaaaagt gctacgccaa aaattatcga cacaagcgca attattgatg
    12121 gtcgtatttt agaagtcatt cgttgcggtt ttatcgatgg caatatttta attccacaag
    12181 gtgttattaa tgaattacaa attgttgcag attcaaatga cagtgttaaa cgtgaaaagg
    12241 gtaaaagagg cttagatatt ttaaatgaat tgtatgattt agactatcct acaaaggtta
    12301 tacatccaac taaaacacat agtgatattg atacgatgtt attaaaacta gcaaaacaat
    12361 atcatgcaag tattataacg acagatttca acctaaataa agtttgtcat gtacatggta
    12421 ttaaagcatt aaatgttaat gatttatcag aagcaatcaa acctaatgta catcaaggtg
    12481 atcaactgca tattttactg acaaaaatgg gtaaggagcc tggtcaggca gtaggatatc
    12541 tagatgatgg tacgatggtg gttgttgata atgctaaaaa tcttattggc agtcatgtca
    12601 atttagaagt agtcagctta ttgcaaacat cttcaggaag aattgttttt gctaaaaaaa
    12661 tcgaagatac agtatcatta taaaaactgt agttgacgaa aacaaaatag atattaaaag
    12721 gtatatgaaa tggcgaagtt tgtaatttga agtataacta taaataaatg caaaacatat
    12781 cagttatcaa aaaatgcaaa tagtgctaat atatcattga aacaagttca ttaagtgtta
    12841 aattttagat attaataaag ttattcttat agcaatcaag acatacaaaa ttagaatcaa
    12901 ttttaacgta tttgaatagt taatttgaag tttaatttca cagtacataa tattcatttt
    12961 taagtcgttg aaatgtttat gtagtgcagt cttgattctt agggatagct tttttaaagt
    13021 gttgaaaaaa caactgcttt ctatttaaaa tttaagcata ttacccaggg aattatgtta
    13081 tgattaaaca atgaaattat tttaaggtca tgttaaaaag cattgtttcg ttttaacaat
    13141 aagacgagtt agacatttgt tatgacggtc gaagatagca aatgaacaat ttatatgaac
    13201 taacttgaaa atctgatatt taatttagga gtgaaaaaaa tgagcgatcg tataagagta
    13261 agatatgcac caagtccaac tggttatctt catattggta atgcaagaac agcattattc
    13321 aattacttgt atgctaaaca ttacaacgga gattttgtga ttcgaattga agatactgat
    13381 aaaaaacgta atttagaaga tggagaaaca tcacaatttg ataatcttaa atggttagga
    13441 ttagattggg atgagtctgt agataaagac aatggctacg gaccatatcg tcaatctgaa
    13501 cgtcaacata tctaccaacc attaatagat cagttactag cagaagataa agcatataaa
    13561 tgctatatga cagaagaaga attagaagct gaacgtgaag cacaaatcgc tcgtggtgaa
    13621 atgcctcgct atggtggaca acatgcgcat ttgactgaag aacaacgtca acaatttgaa
    13681 gcagaaggac gccaaccatc aattcgtttc cgagtacctc aaaaccaaac gtattcattt
    13741 gatgatatgg taaaaggaaa tatttcattt gattcaaatg gtattggtga ctgggttatc
    13801 gtaaaaaaag atggcattcc aacgtacaat tttgcagtag ctatagatga tcattacatg
    13861 caaatttcag atgtaattcg tggtgatgat catatttcaa acacgcctaa acaaattatg
    13921 atttatgaag catttggctg ggagccacct cgttttggtc atatgtcatt aattgttaat
    13981 gaagaacgta aaaagttaag taaacgtgat gggcaaattt tacaatttat tgagcaatat
    14041 cgtgacttag gttatttacc tgaagcgtta tttaatttta ttgcgttatt aggttggtct
    14101 cctgaaggtg aagaagaaat cttttctaaa gaagaattta tcaaaatctt tgatgaaaag
    14161 cgtttgtcaa aatcaccagc atttttcgat aagcaaaaat tagcatgggt taataaccaa
    14221 tatatgaaac aaaaagatac tgaaacagta ttccaattag cattacctca tttaattaaa
    14281 gcaaatttga ttcctgaggt gccgtcagaa gaggatttat cttggggacg caaattaatt
    14341 gcgctttatc aaaaagaaat gagttatgcc ggtgaaattg tacctttatc agaaatgttc
    14401 tttaaagaaa tgccagctct tggtgaagaa gaacaacaag tgattaatgg agagcaagta
    14461 ccagagttaa tgacgcactt attcagtaaa ttagaagcac ttgaaccatt tgaagcggct
    14521 gaaattaaaa agacaattaa agaagttcaa aaagaaacag gaataaaagg caagcaatta
    14581 tttatgccta ttcgtgttgc tgtaacaggc caaatgcatg gtcctgaatt accaaataca
    14641 attgaagtac ttggtaaaga aaaagtgcta aaccgtttaa aacaatataa gtaatgaaat
    14701 agacaagatc aaggtagtat atacttgaat tttataacgt aaccacatat gataaagttg
    14761 tattaacaaa ttacaacaat atgaataaag tattttatat aaaacgaagg attagtaatt
    14821 aaatttatac gatgcagaga gtgtacggtt gctgtgagta caacgtagaa attaatgaat
    14881 gcaccttcgt aaaatgaatt aaatatataa tgagagtgat gagcattaag ttgacttagt
    14941 ttccttgata atttggaagc gcccgcaata ttattaatgt tattcgctaa attcagagtg
    15001 gaatcgtgcg gaagcgcctc taacaataca atttgtatgt tagtggtgct tttttgatat
    15061 ttaatttcgc aggtacttca attatgtatt tacgtagttc aatcattgag gaggaaatga
    15121 tcttgttaaa aagaatgaga gacgatataa aaatggtatt tgagcaggat ccagcggcac
    15181 gttcaacatt agaagtcatt acaacgtatg caggtttaca tgcagtttgg agtcatttga
    15241 ttgcacataa gttatacaac caaaaaaaat atgttgcagc acgcgcgata tctcaaattt
    15301 caagattttt cacaggtata gaaatccatc caggtgctaa aattggaaag cgtctattta
    15361 tagatcatgg tatgggcgtt gtaataggag aaacatgtac aattggtgat aatgtgacaa
    15421 tctatcaagg cgtgacactt ggtgggacag ggaaagaaag agggaaaaga cacccagata
    15481 taggagacaa tgttttaata gcagccggtg cgaaagtttt aggaaatatt aaaataaatt
    15541 caaatgtaaa tattggtgca aattcagttg ttttacaatc agttccaagt tattcaacgg
    15601 ttgttggtat accaggacat attgttaagc aagatggtgt tcgagttgga aaaacatttg
    15661 atcatcgcca tctacctgat ccaatttatg aacaaattaa gcatttagaa cgacaacttg
    15721 aaaagactag gaatggagag attcaagatg attacattat ataatacgct tacacgtcaa
    15781 aaagaagtgt tcaagcctat agaaccaggg aaagtaaaaa tgtatgtatg tggtcctact
    15841 gtatataact acattcatat tggtaacgca agaccagcaa ttaattatga cgtagtgaga
    15901 cgttactttg aataccaagg atataatgta gaatatgtat caaattttac agacgtagat
    15961 gataaattaa ttaaacgttc tcaagaatta aatcagtctg ttcccgaaat tgcagaaaaa
    16021 tatatcgctg cttttcatga agatgttggt gtgttaaatg ttagaaaagc gacttcaaat
    16081 ccaagggtaa tggaccatat ggatgacatt attcaattta ttaaagattt ggtggatcga
    16141 ggttatgcat atgaaagtgg tggcgatgtt tactttagaa cacgtaaatt tgaaggttat
    16201 ggtaaattaa gtcatcaatc catagatgac ttaaaagtgg gtgctcgtat agatgcagga
    16261 gagcataaag aagatgcact tgattttaca ttgtggaaaa aagcgaagcc tggcgagatt
    16321 agttgggata gcccatttgg tgaaggtaga ccaggatggc atatagaatg ttctgtaatg
    16381 gcatttcatg agctaggacc tacaattgat atacatgcgg gtggttcaga tttacaattt
    16441 ccacatcatg aaaatgaaat agcacaatca gaagcacata atcatgcgcc atttgctaat
    16501 tattggatgc ataatggttt cattaatatt gataatgaaa aaatgagtaa atcactaggc
    16561 aactttattt tagttcacga tattattaaa gaagttgatc cagatgtact aagattcttt
    16621 atgattagcg tacattatag aagcccaatt aactataatc tagaattggt agaatcagca
    16681 cgtagtggac tagagcgtat tcgcaatagt tatcaattaa ttgaagagcg cgcacaaatt
    16741 gctactaata ttgaaaatca acagacatat attgatcaaa ttgatgcgat tttaaatcgt
    16801 tttgaaacag ttatgaatga tgattttaat acagctaatg caattacagc ttggtatgat
    16861 ttagcaaaac ttgcgaataa atatgtacta gagaacacaa catcaacaga agtaattgat
    16921 aaatttaaag cagtttatca aattttcagc gatgttttag gtgtaccgtt aaaatctaaa
    16981 aatgcagatg aattattgga tgaagatgtt gaaaaattaa tcgaagagcg taatgaagca
    17041 aggaaaaaca aagattttgc acgagcagat gaaattcgag acatgctgaa atcacaaaac
    17101 attatattag aagacacacc tcaaggggtt agatttaaac gtggataatc aacaagataa
    17161 tcacattaaa ttattgaatc cattgacctt agcatatatg ggagacgcag tcttagatca
    17221 atatgtacgt acctatatcg ttttaaagct taaaagtaag cctaataaac tacatcaaat
    17281 gtctaaaaaa tatgtatctg ccaaaagtca ggcgcaaacg ttagaatatt taatggagca
    17341 agaatggttt acagacgaag aaatggatat tttgaagcga gggcgtaacg cgaaaagtca
    17401 tactaaagct aaaaacactg atgttcaaac atatcgtaaa agttcagcga tagaagcagt
    17461 gataggtttt ctttatttag aaaaaagaga agaacgatta gaggcattat taaataaaat
    17521 aataacaata gtaaacgaaa ggtagtgacg atgtggaaga tacggttatt gttggtaggc
    17581 atgctgttag agaagcgatt attactgggc atccgataaa taagatattg attcaagaag
    17641 gtattaaaaa gcaacaaatt aatgaaattt taaaaaatgc aaaagatcaa aaaatcattg
    17701 ttcaaactgt accaaaatct aaattagatt ttttagcaaa tgcaccacat cagggtgttg
    17761 cagcgcttat tgcaccatat gaatatgctg acttcgatca atttttaaaa cagcaaaaag
    17821 aaaaagaagg tttatcgaca gtacttatat tagacggctt agaagacccg cataacttgg
    17881 gatcaatttt aagaacagcc gatgcaacgg gagttgatgg tgttattatt cctaaacgtc
    17941 gttcagttac actaacgcaa acagttgcaa aagcctcaac aggtgcaatt gaacatgtac
    18001 cagttattcg agtgacaaat ttagctaaaa ctatcgatga actaaaagat aatggctttt
    18061 gggtagctgg cactgaagct aataatgcaa cagattatag aaatctagaa gcggacatgt
    18121 cattggctat tgtaattggt agcgaaggac agggtatgag tcgcctagta agtgataaat
    18181 gcgattttta tattaagatt ccaatggttg gacatgtaaa cagtttgaat gcttcggttg
    18241 cagcaagttt aatgatgtac gaagtatttc gaaaaagaca tgatgttgga gaaatataat
    18301 gaaagaacgt tacttaatca ttgatggata caatatgata ggacaatcac caacgctaag
    18361 cgccattgca aaagagaatt tagaagaagc tagaatgcaa ttaatagatg caattgcaaa
    18421 ttataatgca gttatttcag atgaaattat ttgtgttttc gatgcttatg accaatcggg
    18481 tgttgaaaga gaatacatgt atcatggcgt taaaacgatt tttaccaagg aaaaagaaac
    18541 agctgatagt ttcatagaac gttatgttta tgaactttat gacaagcata ctaagcatat
    18601 tacagttgta acaagtgata tgagtgagca acatgctatc tttggatcag gtgcatatag
    18661 aatatcatct cgcgaaatgt ggagagattt aaaagaaaat gaaattgatg tgagtaaatc
    18721 attagatgat ataagtgaaa acaagccaag aactcgaatt ccgttatctt ctgaaatcct
    18781 tgcagaattt gaaaaaatac gaagaggaca tcataagaaa tgacatttcc gtcatcttga
    18841 aattttgaat gtaacaatat taaactaacc ttaaatttta gatagaaggg gttagtttaa
    18901 tatttgaaat acgatttgac aactcaagac agtacaatca aacgtaacaa tgcaataaat
    18961 gataaagact tcgaaaagtt agtaatggat ctacaaccat taattattcg acgcatcaaa
    19021 acatttggat ttaatcatta tgatttagaa gacttatatc aagaaatact tatacggatg
    19081 tataggtcgg tccaaacatt tgattttagt ggagagcagc ctttcacaaa ttatgttcaa
    19141 tgtttaatta cgtctgtaaa gtatgattat ttgagaaaat atttagctac aaataaaaga
    19201 atggataatt tgattaatga atatagagtt acgtatccat gtgcaataaa gcgttatgat
    19261 gttgaaaaca attatttgaa taaattagca attaaagagt tgattcgtca gtttaagtat
    19321 ttgagtgtat ttgaaaaaga tgtcatgtat ttaatgtgtg aacaatataa gccgagagaa
    19381 attgctcaac tgatgcatgt aaaagagaaa gtgatttata atgccataca acgatgtaaa
    19441 aataaaataa aacgttattt caaaatgatt tgaaaagcgc cttaggacgt gaattgaatt
    19501 agaacgtgtt acttactgat ggtttgacat ttgttataaa ttttatgtat agtatactgg
    19561 tattataatg aataaaggtg aattattgtg agaaaaatac ctttaaattg tgaagcttgt
    19621 ggcaatagaa attataatgt tcctaagcaa gaaggctcgg caacaagatt aaccttaaag
    19681 aaatattgtc caaaatgtaa cgcgcacaca attcataaag aatcgaaata aatacattcg
    19741 aaataatact ttgataatat gttcaaagga tttggaggtt gagcagatgg ctaaaaaaga
    19801 aagtttcttt aaaggcgtta agtctgaaat ggaaaaaaca agttggccga cgaaagaaga
    19861 gctatttaaa tatactgtaa ttgtagtttc tactgttata ttcttcttag tctttttcta
    19921 tgccttagat ttaggaatta cagcattgaa aaatttatta tttggttaga ggagtgaaga
    19981 catgtctgaa gaagttggcg caaagcgttg gtatgcagtg catacatatt ctggatatga
    20041 aaataaagtt aaaaagaatt tagaaaaaag agtagaatct atgaatatga ctgaacaaat
    20101 ctttagagta gtcataccgg aagaagaaga aactcaagta aaagatggca aagctaaaac
    20161 gactgttaaa aaaacattcc ctggatatgt tttagtggaa ttaatcatga cagatgaatc
    20221 atggtatgtg gtaagaaata caccaggtgt tactggtttt gtaggttctg caggtgcagg
    20281 gtctaagcca aatccattgt taccagaaga agttcgcttc atcttaaaac aaatgggtct
    20341 taaagaaaag actatcgatg ttgaactcga agttggcgag caagttcgta ttaaatcagg
    20401 tccatttgcg aatcaagttg gtgaagttca agaaattgaa acagataagt ttaagctaac
    20461 agtattagta gatatgtttg gccgagaaac accagtagaa gttgaattcg atcaaataga
    20521 aaagctttaa ttaacaatta aagttattaa actaaccaaa agataaaaaa gagtattgat
    20581 tttttaatta gaaaagtgtt aaaattatgt ggtcgcgctt ttagagcgcc catttcgtca
    20641 cgaaatgtta agagtgggag ggcaaaactg agccctgtga ccacatcacg atatcaagga
    20701 ggtgcacatc gtggctaaaa aagtagataa agttgttaaa ttacaaattc ctgcaggtaa
    20761 agcgaatcca gcaccaccag ttggtccagc attaggtcaa gcaggtgtga acatcatggg
    20821 attctgtaaa gagttcaatg cacgtactca agatcaagca ggtttaatta ttccggtaga
    20881 aatcagtgtt tatgaagatc gttcatttac atttattaca aaaactccac cggctccagt
    20941 attacttaaa aaagcagctg gtattgaaaa aggttcaggc gaaccaaaca aaactaaagt
    21001 tgctacagta actaaagatc aagtacgcga aattgctaac agcaaaatgc aagacttaaa
    21061 cgctgctgac gaagaagcag ctatgcgtat tatcgaaggt actgcacgta gtatgggtat
    21121 cgttgtagaa taattttacg aatattaaat ttgattacat gatttaaacg atgaagcaga
    21181 taacagagat aataatgatg aattataaat ataatctgaa tgactagatt aatgattgat
    21241 ttattcataa gattaattct tctgttgtct gctcttaact tgcatatagc aagtaatgtg
    21301 ggaggaaatt ccgctaaaac cactaaagga ggaactataa atggctaaaa aaggtaaaaa
    21361 gtatcaagaa gcagctagta aagttgaccg tactcagcac tacagtgttg aagaagcaat
    21421 taaattagct aaagaaacaa gcattgctaa ctttgacgct tctgttgaag ttgcattccg
    21481 tttaggaatt gatacacgta aaaatgacca acaaatccgt ggtgcagttg tattaccaaa
    21541 cggaactggt aaatcacaaa gtgtattagt attcgctaaa ggtgacaaaa ttgctgaagc
    21601 tgaagcagca ggtgctgact atgtaggtga agcagaatac gttcaaaaaa tccaacaagg
    21661 ttggttcgac ttcgatgtag tagttgctac accagacatg atgggtgaag ttggtaaatt
    21721 aggtcgtgta ttaggaccaa aaggtttaat gccaaaccct aaaactggaa ctgtaacaat
    21781 ggatgttaaa aaagctgttg aagaaatcaa agctggtaaa gtagaatatc gtgctgaaaa
    21841 agctggtatc gtacatgcat caattggtaa agtttcattt actgatgaac aattaattga
    21901 aaacttcaat actttacaag atgtattagc taaagctaaa ccatcatctg ctaaaggtac
    21961 atacttcaaa tctgttgctg taactacaac aatgggtcct ggagttaaaa ttgatactgc
    22021 aagtttcaaa taataaataa tataaacaat tacaggctga
//
LOCUS       DAMZKC010000027        21995 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.027, whole genome shotgun sequence.
ACCESSION   DAMZKC010000027 DAMZKC010000000
VERSION     DAMZKC010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 21995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21995
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.027"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            34..999
                     /locus_tag="POB55_000782"
     CDS             34..999
                     /locus_tag="POB55_000782"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HDI6499766.1"
                     /translation="MLNNEYKNSSLKIFSLKGNEALAQEVADQVGIELGKCSVKRFSD
                     GEIQINIEESIRGCDVFIIQPTSYPVNLHLMELLIMIDACKRASAATINIVVPYYGYA
                     RQDRKARSREPITAKLVANLIETAGATRMIALDLHAPQIQGFFDIPIDHLMGVPILAK
                     HFKDDPNINPEECVVVSPDHGGVTRARKLADILKTPIAIIDKRRPRPNVAEVMNIVGE
                     IEGRTAIIIDDIIDTAGTITLAAQALKDKGAKEVYACCTHPVLSGPAKERIENSAIKE
                     LIVTNSIHLDEDRKPSNTKELSVAGLIAQAIIRVYERESVSVLFD"
     gene            1149..1802
                     /locus_tag="POB55_000783"
     CDS             1149..1802
                     /locus_tag="POB55_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HDI6499767.1"
                     /translation="MASLKSIIRQGKQTRSDLKQLRKSGKVPAVVYGYGTKNVSVKVD
                     EVEFIKVIREVGRNGVIELGVGSKTIKVMVADYQFDPLKNQITHIDFLAINMSEERTV
                     EVPVQLVGEAVGAKEGGVVEQPLFNLEVTATPDNIPEAIEVDITELNINDSLTVADVK
                     VTGDFKIENDSAESVVTVVAPTEEPTEEEIEAMEGEQQTEEPEVVGESKEDEEKTEE"
     gene            2113..2685
                     /locus_tag="POB55_000784"
     CDS             2113..2685
                     /locus_tag="POB55_000784"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDI6499768.1"
                     /translation="MKCIVGLGNIGKRFELTRHNIGFEVVDYILEKNNFSLDKQKFKG
                     AYTIERMNGDKVLFIEPMTMMNLSGEAVAPIMDYYNVNPEDLIVLYDDLDLEQGQVRL
                     RQKGSAGGHNGMKSIIKMLGTDQFKRIRIGVGRPTNGMTVPDYVLQRFSNDEMVTMEK
                     VIEHAARAIEKFVETSRFDHVMNEFNGEVK"
     gene            2685..6191
                     /gene="mfd"
                     /locus_tag="POB55_000785"
     CDS             2685..6191
                     /gene="mfd"
                     /locus_tag="POB55_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HDI6499769.1"
                     /translation="MTILTTLIKEDNHFQDLNQVFGQANTLVTGLSPSAKVTMIAEKY
                     AQSNQQLLLITNNLYQADKLETDLLQFIDAEELYKYPVQDIMTEEFSTQSPQLMSERI
                     RTLTALAQGKKGLFIVPLNGLKKWLTPVEMWQNHQMTLRVGEDIDVDQFLNKLVNMGY
                     KRESVVSHIGEFSLRGGIIDIFPLIGEPIRIELFDTKIDSIRDFDVETQRSKDNVEEV
                     DITTASDYIITEEVISHLKEELKTAYENTRPKIDKSVRNDLKETYESFKLFESTYFDH
                     QILRRLVAFMYETPSTIIEYFQKDAIIAVDEFNRIKETEESLTVESDSFISNIIESGN
                     GFIGQSFIKYDDFETLIEGYPVTYFSLFATTMPIKLNHIIKFSCKPVQQFYGQYDIMR
                     SEFQRYVNQNYHIVVLVETETKVERMQAMLSEMHIPSITKLHRSMSSGQAVIIEGSLS
                     EGFELPDMGLVVITERELFKSKQKKQRKRTKAISNAEKIKSYQDLNVGDYIVHVHHGV
                     GRYLGVETLEVGQTHRDYIKLQYKGTDQLFVPVDQMDQVQKYVASEDKTPKLNKLGGS
                     EWKKTKAKVQQSVEDIAEELIDLYKEREMAEGYQYGEDTAEQTTFELDFPYELTPDQA
                     KSIDEIKDDMQKSRPMDRLLCGDVGYGKTEVAVRAAFKAVMEGKQVAFLVPTTILAQQ
                     HYETLIERMQDFPVEIQLMSRFRTPKEIKQTKEGLKTGFVDIVVGTHKLLSKDIQYKD
                     LGLLIVDEEQRFGVRHKERIKTLKHNVDVLTLTATPIPRTLHMSMLGVRDLSVIETPP
                     ENRFPVQTYVLEQNMSFIKEALERELSRDGQVFYLYNKVQSIYEKREQLQMLMPDANI
                     AVAHGQMTERDLEETMLSFINNEYDILVTTTIIETGVDVPNANTLIIEDADRFGLSQL
                     YQLRGRVGRSSRIGYAYFLHPANKVLTETAEDRLQAIKEFTELGSGFKIAMRDLNIRG
                     AGNLLGKQQHGFIDTVGFDLYSQMLEEAVNEKRGIKEPESEVPEVEVDLNLDAYLPTE
                     YIANEQAKIEIYKKLRKTETFDQIIDIKDELIDRFNDYPVEVARLLDIVEIKVHALHS
                     GITLIKDKGKIIDIHLSVKATENIDGEVLFKATQPLGRTMKVGVQNNAMTITLTKQNQ
                     WLDSLKFLVKCIEESMRISDEA"
     gene            6181..7707
                     /locus_tag="POB55_000786"
     CDS             6181..7707
                     /locus_tag="POB55_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDI6499770.1"
                     /translation="MKHKEAFNGVVVLTAALIVIKILSAVYRIPYQNILGDTGLYAYQ
                     QVYPIVALGMILSMNAIPSAITQNIGKYHSDEAYAKAVAYIQLVGILLFIAIFVFANN
                     IAHMMGDSHLTPMIQAASLSFIFIGMLGVLRGYYQSANNMTVPAISQVIEQVIRVGII
                     IVTIVIFVDRGWTIYEAGTIAILASTIGFLGSSIYLVAHRPFKFKMVNNTAKIVWKQF
                     ALSVLIFAISQLIVILWQVIDSVTIIKSLQAIRVPFDVAITEKGVYDRGASFIQMGLI
                     VTTTFSFALIPLLSDAIKMNNQVLMNRYANASLKITILISTAAGIGLINLLPLMNGVF
                     FKTNDLTLTLSVYMITVICVSLIMMDMALLQAQHAVRPIFVGMTAGLVIKFILNIILI
                     RLSGIIGASISTVVSLIIFGTIIHIAVTRKYHLHAMRRFFINVVLGMVFMSIVVQCVL
                     NIVTTHGRFTGLIELLCAAVLGIIALFFYIFRFNVLTYKELTYLPFGSKLYQIKKGRR
                     "
     gene            7707..8900
                     /locus_tag="POB55_000787"
     CDS             7707..8900
                     /locus_tag="POB55_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HDI6499771.1"
                     /translation="MAHTITIVGLGNYGIDDLPLGIYKFLKTQDKVYARTLDHPVIES
                     LQDELTFQSFDHVYEAHDQFEDVYNDIVAQLVEAANEKDIVYAVPGHPRVAETTTVKL
                     LALAKDNTDIDVKVLGGKSFIDDVFEAVNVDPNDGFTLLDATSLQEVTLNVRTHTLIT
                     QVYSAMVAANLKITLMERYPDDYPVQIVTGARSDGADNVVTCPLYELDHDENAFNNLT
                     SVFVPKIITSTYLYHDFDFATEVIDTLVDEDKGCPWDKVQTHETLKRYLLEETFELFE
                     AIDNEDDWHMIEELGDILLQVLLHTSIGKKEGYIDIKEVITSLNAKMIRRHPHIFGDA
                     NAETIDDLKEIWSKAKDAEGKQPRVKFEKVFAEHFLNLYEKTKDKSFDEASLKQWLEK
                     GESNT"
     gene            8897..9160
                     /locus_tag="POB55_000788"
     CDS             8897..9160
                     /locus_tag="POB55_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HDI6499772.1"
                     /translation="MRLDKYLKVSRLIKRRTLAKEVSDQGRITINGNVAKAGSDVKVE
                     DVLTIRFGQKLVTVKVTALNEHASKDNAKGMYEIIEERRLEEA"
     gene            9178..9570
                     /locus_tag="POB55_000789"
     CDS             9178..9570
                     /locus_tag="POB55_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HDI6499773.1"
                     /translation="MKNKVEHIENQYTSQENKKKQRQKMKMRVVRRRITVFAGVLLAI
                     IVVLSILLVVQKHRNDIDAQERKAKEAQFQKQQNEEIALKEKLNNLNDKDYIEKIARD
                     DYYLSNKGEVIFRLPEDKDSSSSKSSKK"
     gene            9675..10076
                     /locus_tag="POB55_000790"
     CDS             9675..10076
                     /locus_tag="POB55_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HDI6499774.1"
                     /translation="MSIEVGNKLKGKVTGIKKFGAFVELPEGKSGLVHISEVADNYVE
                     NVEEHLSVGDEVDVKVLSIADDGKISLSIKKAKDRPRRQHTSKPSHQKPVQKAEDFEK
                     KLSNFLKDSEDKLTSIKRQTESRRGGKGSRR"
     gene            10256..11551
                     /gene="tilS"
                     /locus_tag="POB55_000791"
     CDS             10256..11551
                     /gene="tilS"
                     /locus_tag="POB55_000791"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HDI6499775.1"
                     /translation="MQLNSNGWHVDDHIVVAVSTGIDSMCLLYQLLNDYKDSYRKLTC
                     LHVNHGVRSASIEEARFLEVYCERHHIDLHIKKLDLSHSLDRNNSIQNEARIKRYEWF
                     DEMMNVLEADVLLTAHHLDDQLETIMYRIFNGKSTRNKLGFDELSKRKGYQIYRPLLA
                     VSKKEIKQFQERYHIPYFEDESNKDNKYVRNDIRNRIIPAIDENNQLKASHLLKLKQW
                     HDEQYDILQYSAKQFIQEFVKFDEQSKYLEVSRQAFNNLPNSLKMVVLDCLLSKYYEL
                     FNISAKTYEEWFKQFSSKKAQFSINLTDKWIIQIAYGKLIIMAKNNGDTYFRVQTIEK
                     PGNYIFNKYRLEIHSNLPKCLFPLTVRTRQSGDTFKLNGRDGYKKVNRLFIDCKVQQW
                     VRDQMPIVLDKQQRIIAVGDLYQQQTIKQWIIISKNGDE"
     gene            11556..12095
                     /gene="hpt"
                     /locus_tag="POB55_000792"
     CDS             11556..12095
                     /gene="hpt"
                     /locus_tag="POB55_000792"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDI6499776.1"
                     /translation="MHNDLKEVLLTEEDIQNICKELGAQLTKDYQGKPLVCVGILKGS
                     AMFMSDLIKRIDTHLSIDFMDVSSYHGGTESTGEVQIIKDLGSSIENKDVLIIEDILE
                     TGTTLKSITELLQSRKVNSLEIVTLLDKPNRRKADIEAKYVGKKIPDEFVVGYGLDYR
                     ELYRNLPYIGTLKPEVYSN"
     gene            12352..14445
                     /gene="ftsH"
                     /locus_tag="POB55_000793"
     CDS             12352..14445
                     /gene="ftsH"
                     /locus_tag="POB55_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDI6499777.1"
                     /translation="MQKAFRNVLVIVIIGVIIFGLFSYLNGNGNMPKQLTYNQFTEKL
                     EKGDLKTLEIQPQQNVYMVSGKTKNDEDYSSTILYNNEKELQKITDAAKKQNGVKLTI
                     KEEEKQSVFVSILSTLIPVVVIALLFIFFLSQAQGGGSGGRMMNFGKSKAKMYDNNKR
                     RVRFSDVAGADEEKQELIEIVDFLKDNKKFKEMGSRIPKGVLLVGPPGTGKTLLARAV
                     AGEAGAPFFSISGSDFVEMFVGVGASRVRDLFDNAKKNAPCIIFIDEIDAVGRQRGAG
                     VGGGHDEREQTLNQLLVEMDGFGENEGIIMIAATNRPDILDPALLRPGRFDRQIQVGR
                     PDVKGREAILHVHAKNKPLDETVDLKAISQRTPGFSGADLENLLNEASLIAVREGKKK
                     IDMRDIEEATDRVIAGPAKKSRVISKKERNIVAHHEAGHTIIGMVLDEAEVVHKVTIV
                     PRGQAGGYAMMLPKQDRFLMTEQELLDKICGLLGGRVSEDINFNEVSTGASNDFERAT
                     QIARSMVTQYGMSKKLGPLQFGHSNGQVFLGKDMQGEPNYSSQIAYEIDKEVQRIVKE
                     QYERCKQILLEHKEQLILIAETLLTEETLVAEQIQSLFYEGKLPEIDYDAAKVVKDED
                     SEFNDGKFGKSYEEIRKEQLEDGQRDESEDRKEEKDIAEDKKEADKSDEKDEPAHRQA
                     PNIEKPYDPNHPDNK"
     gene            14674..15555
                     /gene="hslO"
                     /locus_tag="POB55_000794"
     CDS             14674..15555
                     /gene="hslO"
                     /locus_tag="POB55_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HDI6499778.1"
                     /translation="MTHDYIVKALAFDGEIRAYAALTTETVQEAQTRHYTWPTASAAM
                     GRTMTATAMMGAMLKGDQKLTVTVDGQGPIGRIIADANAKGEVRAYVDHPQTHFPLNE
                     QGKLDVRRAVGTNGSIMVVKDVGMKDYFSGASPIVSGELGEDFTYYYATSEQTPSSVG
                     LGVLVNPDNTIKAAGGFIIQVMPGAKDETISKLEKAISEMTPVSKLIEQGLTPEGLLN
                     EILGEDHVQILEKMPVQFECNCSHEKFLNAIKGLGEAEIQNMIKEDHGAEAVCHFCGN
                     KYKYTEEELNVLLESLA"
     gene            15734..16666
                     /gene="cysK"
                     /locus_tag="POB55_000795"
     CDS             15734..16666
                     /gene="cysK"
                     /locus_tag="POB55_000795"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HDI6499779.1"
                     /translation="MAQKPVDNITQIIGGTPVVKLRNVVDDNAADVYVKLEYQNPGGS
                     VKDRIALAMIEKAEREGKIKPGDTIVEPTSGNTGIGLAFVCAAKGYKAVFTMPETMSQ
                     ERRNLLKAYGAELVLTPGSEAMKGAIKKAKELKEEHGYFEPQQFENPANPEVHELTTG
                     PELLQQFEGKTIDAFLAGVGTGGTLSGVGKVLKKEYPNIEIVAIEPEASPVLSGGEPG
                     PHKLQGLGAGFIPGTLNTEIYDSIIKVGNDTAMEMSRRVAKEEGILAGISSGAAIYAA
                     IQKAKELGKGKTVVTVLPSNGERYLSTPLYSFDD"
     gene            16882..17685
                     /gene="folP"
                     /locus_tag="POB55_000796"
     CDS             16882..17685
                     /gene="folP"
                     /locus_tag="POB55_000796"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HDI6499780.1"
                     /translation="MTKTKIMGILNVTPDSFSDGGKFNNVESAINRVKAMIDEGVDII
                     DVGGVSTRPGHEMVSLEEEMNRVLPVVEAIVGFDVKISVDTFRSEVAEACLKLGVDMI
                     NDQWAGLYDHRMFQIVAKYDAEIILMHNGNGNRDEPVVEEMLTSLLAQAHQAKIAGIP
                     SNKIWLDPGIGFAKTRNEEAEVMARLDELVATEYPVLLATSRKRFTKEMMGYDTTPVE
                     RDEVTAATTAYGIMKGVRAVRVHNVELNAKLAKGIDFLKENENARHNLS"
     gene            17663..18028
                     /gene="folB"
                     /locus_tag="POB55_000797"
     CDS             17663..18028
                     /gene="folB"
                     /locus_tag="POB55_000797"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HDI6499781.1"
                     /translation="MQDTIFLKGMRFYGYHGALSAENEIGQIFKVDVTLKVDLAEAGR
                     TDNVIDTVHYGEVFEEVKSIMEGKAVNLLEHLAERIANRINSQYNRVMETKVRITKEN
                     PPIPGHYDGVGIEIVRENK"
     gene            18025..18501
                     /gene="folK"
                     /locus_tag="POB55_000798"
     CDS             18025..18501
                     /gene="folK"
                     /locus_tag="POB55_000798"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HDI6499782.1"
                     /translation="MIQAYLGLGSNIGDRESQLNDAIKILNEYDGINVSNISPIYETA
                     PVGYTEQPNFLNLCVEIQTTLTVLQLLECCLKTEECLHRIRKERWGPRTLDVDILLYG
                     EEMIDLPKLSVPHPRMNERAFVLIPLNDIAANVVEPRSKLKVKDLVFVDDSLKRYK"
     gene            18895..18987
                     /locus_tag="POB55_000799"
     CDS             18895..18987
                     /locus_tag="POB55_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499783.1"
                     /translation="MVNDKVLETSKEMYVEQKCLIFYKTLKENV"
     gene            19040..20527
                     /gene="lysS"
                     /locus_tag="POB55_000800"
     CDS             19040..20527
                     /gene="lysS"
                     /locus_tag="POB55_000800"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDI6499784.1"
                     /translation="MSEEMNDQMLVRRQKLQELYDLGIDPFGSKFDRSGLSSDLKEEW
                     DQYSKEELVEKEADSHVAIAGRLMTKRGKGKAGFAHVQDLAGQIQIYVRKDQVGDDEF
                     DLWKNADLGDIVGVEGVMFKTNTGELSVKAKKFTLLTKSLRPLPDKFHGLQDIEQRYR
                     QRYLDLITNEDSTRTFINRSKIIQEMRNYLNNKGFLEVETPMMHQIAGGAAARPFVTH
                     HNALDATLYMRIAIELHLKRLIVGGLEKVYEIGRVFRNEGVSTRHNPEFTMIELYEAY
                     ADYHDIMDLTESMVRHIANEVLGSAKVQYNGETIDLESAWTRLHIVDAVKEATGVDFY
                     EVKSDDEAKALAKEHGIEIKDTMKYGHILNEFFEQKVEETLIQPTFIYGHPTEISPLA
                     KKNPEDPRFTDRFELFIVGREHANAFTELNDPIDQKGRFEAQLVEKAQGNDEAHEMDE
                     DYIEALEYGMPPTGGLGIGIDRLVMLLTDSPSIRDVLLFPYMRQK"
     gene            21085..21199
                     /gene="rrf"
                     /locus_tag="POB55_000801"
     rRNA            21085..21199
                     /gene="rrf"
                     /locus_tag="POB55_000801"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            21212..21287
                     /locus_tag="POB55_000802"
     tRNA            21212..21287
                     /locus_tag="POB55_000802"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21245..21247,aa:Val,seq:tac)
     gene            21304..21379
                     /locus_tag="POB55_000803"
     tRNA            21304..21379
                     /locus_tag="POB55_000803"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21337..21339,aa:Thr,seq:tgt)
     gene            21386..21458
                     /locus_tag="POB55_000804"
     tRNA            21386..21458
                     /locus_tag="POB55_000804"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21419..21421,aa:Lys,seq:ttt)
     gene            21492..21566
                     /locus_tag="POB55_000805"
     tRNA            21492..21566
                     /locus_tag="POB55_000805"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21524..21526,aa:Gly,seq:gcc)
     gene            21574..21662
                     /locus_tag="POB55_000806"
     tRNA            21574..21662
                     /locus_tag="POB55_000806"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21608..21610,aa:Leu,seq:taa)
     gene            21668..21744
                     /locus_tag="POB55_000807"
     tRNA            21668..21744
                     /locus_tag="POB55_000807"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21702..21704,aa:Arg,seq:acg)
     gene            21765..21838
                     /locus_tag="POB55_000808"
     tRNA            21765..21838
                     /locus_tag="POB55_000808"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21799..21801,aa:Pro,seq:tgg)
     gene            21862..21937
                     /locus_tag="POB55_000809"
     tRNA            21862..21937
                     /locus_tag="POB55_000809"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21895..21897,aa:Ala,seq:tgc)
BASE COUNT         7799 a         3025 c         4484 g         6687 t
ORIGIN      
        1 taattgcgta agtaaataat tggaggacta taaatgttaa ataatgaata taagaattcg
       61 tcattaaaga ttttttcatt gaaaggaaac gaagcattag cgcaagaagt tgctgaccaa
      121 gtaggaattg aactaggtaa atgttcagtt aaacgtttta gtgatggaga aattcaaatt
      181 aatatcgaag agagtattcg tggttgtgac gtatttatta ttcaaccaac atcatatcct
      241 gtgaatctac atttaatgga attattaatt atgattgacg cttgtaaacg tgcttctgca
      301 gcaacaatca atattgtagt gccatattat ggatatgcaa gacaagatag aaaagcccgt
      361 agccgtgagc caatcacagc taaattagtt gcaaacttaa tcgaaacagc tggcgcaact
      421 cgtatgattg cgttagactt acatgcacca caaattcaag gattctttga tattccaatt
      481 gaccacttaa tgggtgtgcc aattcttgct aaacatttca aagatgatcc gaatattaac
      541 ccagaagaat gtgtcgttgt ttcaccagac catggcggtg ttacacgtgc acgtaaatta
      601 gctgacattt taaaaactcc aattgcaatt atagataaac gtcgtcctag accaaatgtt
      661 gctgaagtga tgaacattgt tggtgagatt gaaggacgta cggcaattat tattgacgat
      721 attattgata cagcaggtac aatcacttta gctgcacaag cattaaaaga taaaggtgct
      781 aaagaagtat atgcttgttg tacacaccct gttttatcag gaccggctaa agaacgtatc
      841 gaaaattctg ctataaaaga attaatcgta acaaactcaa ttcatttaga tgaagaccgc
      901 aaaccatcta acactaaaga attatctgtt gctggtttaa tcgcacaagc tatcattcgt
      961 gtatacgaaa gagaatcagt tagcgtatta tttgactaat atttaaaagg cgtttgacga
     1021 acatattcca aacgtgtata atagtttcgt tcgtgattat acgaataaat aaacacttgc
     1081 aagcaacgat gatgttgatg ggtaagtgag gtgctcgttt tgagcaaaaa tgaaaggtgg
     1141 aaatgagaat ggcttcatta aagtcaatca tccgtcaagg taaacaaaca cgttcagatc
     1201 ttaaacaatt aagaaaatct ggtaaagtac cagcagtagt atacggttac ggtactaaaa
     1261 acgtgtcagt taaagttgat gaagtagaat tcatcaaagt tatccgtgaa gtaggtcgta
     1321 acggtgttat cgaattaggc gttggttcta aaactatcaa agttatggtt gcagactacc
     1381 aattcgatcc acttaaaaac caaattactc acattgactt cttagcaatc aatatgagtg
     1441 aagaacgtac tgttgaagta ccagttcaat tagttggtga agcagtaggc gctaaagaag
     1501 gcggcgtagt tgaacaacca ttattcaact tagaagtaac tgctactcca gacaatattc
     1561 cagaagcaat cgaagtagac attactgaat taaacattaa cgacagctta actgttgctg
     1621 atgttaaagt aactggcgac ttcaaaatcg aaaacgattc agctgaatca gtagtaacag
     1681 tagttgctcc aactgaagaa ccaactgaag aagaaatcga agctatggaa ggcgaacaac
     1741 aaactgaaga accagaagtt gttggcgaaa gcaaagaaga cgaagaaaaa actgaagagt
     1801 aattttaatc tgttacatta aagtttttat actttgttta acaagcactg tgcttatttt
     1861 aatataagca tggtgctttt tgtgttatta taaagcttaa ttaaacttta ttgctttgta
     1921 ataaagttta attaatttta gtgagtaaaa gacattaaac tcaacaatga tacatcataa
     1981 aaattttaat gtactcgatt ttaaaataca tacttactaa gctaaagaat aatgataatt
     2041 gatggcaatg gcggaaaata gatgttgtca ttataataat aaatgaaaca attatgttgg
     2101 aggtaaacac gcatgaaatg tattgtaggt ctaggtaata taggtaaacg ttttgaactt
     2161 acaagacata atatcggctt tgaagtcgtt gattatattt tagagaaaaa taatttttca
     2221 ttagataaac aaaagtttaa aggtgcatat acaattgaac gaatgaacgg cgataaagtg
     2281 ttatttatcg aaccaatgac aatgatgaat ttgtcaggag aagcagttgc accgattatg
     2341 gattattaca atgttaatcc agaagattta attgtcttat atgatgattt agatttagaa
     2401 caaggacaag ttcgcttaag acaaaaagga agtgcgggcg gtcacaatgg tatgaaatca
     2461 attattaaaa tgcttggtac agaccaattt aaacgtattc gtattggtgt gggaagacca
     2521 acgaatggta tgacggtacc tgattatgtt ttacaacgct tttcaaatga tgaaatggta
     2581 acgatggaaa aagttatcga acacgcagca cgcgcaattg aaaagtttgt tgaaacatca
     2641 cgatttgacc atgttatgaa tgaatttaat ggtgaagtga aataatgaca atattgacaa
     2701 cgcttataaa agaagataat cattttcaag accttaatca ggtatttgga caagcaaaca
     2761 cactagtaac tggtctttcc ccgtcagcta aagtgacgat gattgctgaa aaatatgcac
     2821 aaagtaatca acagttatta ttaattacca ataatttata ccaagcagat aaattagaaa
     2881 cagatttact tcaatttata gatgctgaag aattgtataa gtatcctgtg caagatatta
     2941 tgaccgaaga gttttcaaca caaagccctc aactgatgag tgaacgtatt agaactttaa
     3001 ctgcgttagc tcaaggtaag aaagggttat ttatcgttcc tttaaatggt ttgaaaaagt
     3061 ggttaactcc tgttgaaatg tggcaaaatc accaaatgac attgcgtgtt ggtgaggata
     3121 tcgatgtgga ccaatttctt aacaaattag ttaatatggg gtacaaacgg gaatccgtgg
     3181 tatcgcatat tggtgaattc tcattgcgag gaggtattat cgatatcttt ccgctaattg
     3241 gggaaccaat cagaattgag ctatttgata ccaaaattga ttctattcgg gattttgatg
     3301 ttgaaacgca gcgttccaaa gataatgttg aagaagtcga tatcacaact gcaagtgatt
     3361 atatcattac tgaagaagtg atcagccatc ttaaagaaga gttaaaaact gcatatgaaa
     3421 atacaagacc caaaatagat aaatcagtgc gcaatgattt gaaagaaacg tatgaaagct
     3481 ttaaattatt cgaaagtaca tactttgatc atcaaatact acgtcgctta gtagcgttta
     3541 tgtatgaaac accttcgaca attattgagt atttccaaaa agatgcaatc attgcagttg
     3601 atgaatttaa tcgtattaaa gaaactgaag aaagtttaac agtagagtct gattcgttta
     3661 ttagcaatat tattgaaagt ggtaatggat ttataggaca aagttttata aaatatgatg
     3721 attttgaaac attgattgaa ggctatcctg tcacttattt ttcattattc gctacaacaa
     3781 tgccgataaa actaaatcat attattaaat tttcatgtaa acctgttcaa caattttatg
     3841 ggcaatatga cattatgcgt tctgaatttc aacgatatgt taatcaaaac tatcatatcg
     3901 tggttttggt cgaaaccgaa actaaagttg aacgtatgca agcgatgtta agtgaaatgc
     3961 atattccatc aataacaaaa ttgcatcgct caatgtcatc gggacaagca gtgattattg
     4021 agggcagttt atctgaagga tttgaactac ctgatatggg attagttgtc attactgagc
     4081 gtgagctttt taaatcaaaa cagaaaaagc aacgaaaacg tacgaaagct atctcaaatg
     4141 ctgaaaaaat taagtcttac caagatttaa atgtgggaga ttatattgtt catgtgcatc
     4201 atggtgttgg tagatattta ggtgttgaga cgctcgaagt ggggcaaacg catcgtgatt
     4261 atattaaatt gcaatataaa ggtacggatc aactatttgt tccagtagat caaatggatc
     4321 aagttcaaaa atatgtagct tcggaagata agacgccaaa attaaataaa ctcggtggca
     4381 gtgaatggaa aaaaacaaaa gctaaagttc aacaaagtgt tgaagatatt gctgaagagt
     4441 tgattgattt atataaagaa agagaaatgg cagaaggtta tcaatatggg gaagacacag
     4501 ctgagcaaac aacatttgaa ttagattttc catatgaact tacgcctgac caagctaaat
     4561 ctatcgatga aattaaagat gacatgcaaa aatcgcgtcc aatggatcgc ttgctatgtg
     4621 gtgatgttgg ttatggtaaa actgaagttg cagtgagagc agcattcaaa gctgtaatgg
     4681 aaggaaagca ggttgcattt ttagttccta caactatttt agctcagcaa cattatgaga
     4741 cgttaattga gcgtatgcaa gattttcctg ttgaaattca attaatgagt cgttttagaa
     4801 cgcctaaaga gataaaacaa actaaggaag gacttaaaac tggatttgtt gacatagttg
     4861 ttggtacaca caaattactt agtaaagata tacagtataa agatttaggg ctgttgattg
     4921 tagatgaaga acaacgattt ggtgtacgcc ataaagagcg tattaaaaca ttaaaacata
     4981 atgtagatgt actaacattg actgcaaccc caatacctag aacattgcat atgagtatgc
     5041 taggtgtgcg cgatttgtca gtgattgaaa cgccgccaga aaatcgtttc ccagttcaaa
     5101 catatgtatt agaacagaac atgagtttta tcaaagaagc tttagaaaga gaactatccc
     5161 gtgatggcca agtgttttat ctttataata aagtgcaatc catttatgaa aaacgagaac
     5221 aactccagat gttaatgcca gatgctaaca ttgcagttgc tcatggacaa atgacagagc
     5281 gcgatttaga agaaacgatg ttaagtttta tcaataatga atatgatatt ttagtaacga
     5341 cgacgattat tgaaacaggt gtcgatgtcc caaatgcaaa tactttgatc attgaagatg
     5401 cagatcgctt tggattgagt cagttgtatc aattaagagg tcgtgttggt cgttcaagtc
     5461 gtattggtta tgcatacttc ttacatccag caaataaggt actaactgag actgcagaag
     5521 atcgattaca agcgattaaa gaatttacgg agttaggctc aggatttaag attgcgatgc
     5581 gtgatttgaa cattcgtggt gctggtaatt tgttaggtaa acaacagcac ggctttattg
     5641 atacagttgg atttgatttg tacagtcaaa tgttagaaga agctgtaaat gaaaaacgtg
     5701 gtattaagga accagaatct gaggtgccag aagtcgaagt tgatttaaac ttggatgcat
     5761 atttgccgac agaatatatt gcaaatgaac aagctaaaat tgaaatttat aaaaagctac
     5821 gaaaaactga aacatttgat caaattatcg acattaaaga tgaattaatt gatcgtttta
     5881 atgattatcc tgttgaagta gcacgtttgc ttgatatagt ggaaataaaa gtacacgcat
     5941 tacattcagg tatcacgttg attaaagata aagggaaaat aattgatatt catttatctg
     6001 taaaagccac tgaaaatatt gatggcgaag tgctgttcaa agcaacacaa cctttaggta
     6061 gaacaatgaa ggttggtgtt caaaataatg caatgacaat tactttaacg aaacaaaatc
     6121 aatggcttga tagtttgaag tttttagtta agtgcattga agaaagtatg agaatcagtg
     6181 atgaagcata aagaagcatt taatggcgtt gtcgtgttaa ctgctgcatt aattgtcatt
     6241 aaaattctga gtgctgtata tcgaattcca tatcaaaata tattaggtga tacaggtttg
     6301 tatgcatatc aacaagtgta tccaattgta gcattaggaa tgatattatc gatgaatgcc
     6361 attcctagtg caattacaca aaatataggg aagtatcata gtgacgaagc atatgcaaaa
     6421 gcggtcgctt atatacaatt agttggtata ttattattta ttgctatttt tgtgtttgcg
     6481 aacaatattg cacatatgat gggtgatagc catttaacac caatgattca agcagcaagt
     6541 ttaagcttta tatttatagg tatgcttggc gtgttaagag gttattatca atctgcaaat
     6601 aatatgacag ttccggctat ttcccaggtt atagaacaag ttatacgagt aggtattatc
     6661 attgttacta ttgttatttt tgtagacaga ggttggacga tatatgaagc gggaacaatt
     6721 gctattttag catcaacgat aggtttttta ggttcttcaa tttatttagt agcgcaccga
     6781 ccttttaagt ttaaaatggt aaataacact gcaaagattg tttggaaaca gttcgcactt
     6841 tcggttttga ttttcgctat cagtcaatta atcgtaattt tatggcaagt gattgatagt
     6901 gttactatta ttaagtcact tcaagcgata cgcgtgccat tcgatgttgc cataactgaa
     6961 aaaggagtct atgaccgtgg tgcatcattt attcagatgg gattgattgt aactacaaca
     7021 tttagttttg cgctcattcc tctgttaagt gacgcaatca aaatgaataa tcaggtactt
     7081 atgaatcgtt atgcaaatgc gtcattaaag attacgattt taataagtac agcagcggga
     7141 ataggattaa ttaatttatt gcctttaatg aacggtgtgt tttttaagac gaatgattta
     7201 accttaacgt taagtgttta tatgattacg gtcatttgtg tatcgttaat tatgatggat
     7261 atggctttat tacaagcgca acatgctgtg agacctattt ttgttggtat gacggcagga
     7321 ttggttatta aatttatact taatatcatt ttgattcgtt taagtggcat tattggtgcg
     7381 agcattagta ctgttgtatc attaattata ttcggtacga ttatccatat tgctgtcacg
     7441 agaaaatacc acttacatgc gatgagacga ttttttatca atgttgtttt aggtatggta
     7501 tttatgtcga ttgttgttca atgcgtgtta aacatagtga caacacacgg tagattcact
     7561 ggactcattg aattattatg tgcagcagta ttaggtatca ttgcattgtt tttctatatt
     7621 tttagattta atgttttgac atataaagag ttaacttatt taccatttgg ttcaaagttg
     7681 tatcaaatta agaaaggaag acgttgatgg cacataccat tacgattgtt ggcttaggaa
     7741 actatggcat tgatgatttg ccgctaggga tatataaatt tttaaagaca caagataaag
     7801 tttatgcaag aacgttagat catccagtta tagaatcatt gcaagatgaa ttaacatttc
     7861 agagttttga ccatgtttat gaagcacatg accaatttga agatgtctat aatgatattg
     7921 tggcgcaatt ggttgaagct gctaatgaaa aagatattgt ctatgcggtt ccgggtcatc
     7981 ctagagttgc tgagacaact acagtgaaat tactggcttt agcaaaggac aatactgata
     8041 tagatgtgaa agttttaggt ggtaaaagct ttattgatga tgtgtttgaa gcagttaatg
     8101 tagatccaaa tgatggcttc acactgttag atgcgacatc attacaagaa gtaacactta
     8161 atgttagaac gcatacattg attacacaag tttatagtgc aatggttgct gctaatttga
     8221 aaatcacttt aatggaacga tatcctgatg attaccctgt tcaaattgtc actggtgcac
     8281 gaagcgatgg tgcggataac gttgtgacat gcccattata tgaattggat catgatgaaa
     8341 atgcattcaa taatttgacg agtgtattcg taccaaaaat cataacatcg acatatttgt
     8401 atcatgactt tgattttgca acggaagtga ttgatacttt agttgatgaa gataaaggtt
     8461 gtccatggga taaagtgcaa acgcatgaaa cgcttaagcg ttatttactt gaagaaacat
     8521 ttgaattgtt cgaagctatt gacaatgaag atgattggca tatgattgaa gagctaggag
     8581 atattttatt acaagtgtta ttgcatacta gtattggtaa aaaagaaggg tatatcgaca
     8641 ttaaagaagt gattacaagt cttaatgcta aaatgattcg tagacaccca cacatatttg
     8701 gtgatgccaa tgctgaaact atcgatgact taaaagaaat ttggtctaag gcgaaagatg
     8761 ctgaaggtaa acagccaaga gttaaatttg aaaaagtatt tgcagagcat tttttaaatt
     8821 tatatgagaa gacgaaggat aagtcatttg atgaggcctc gttaaagcag tggctagaaa
     8881 aaggggagag taatacatga gattagataa atatttaaaa gtatcacggt taataaagcg
     8941 acgtacgcta gcaaaagaag taagtgatca aggtagaatt acaataaatg gtaatgttgc
     9001 taaagctgga tcggatgtta aagttgaaga tgtgctgacg attcgctttg gtcaaaaatt
     9061 agtaacagtt aaagtaactg cattaaatga acatgcatct aaagataacg cgaagggcat
     9121 gtatgaaatc attgaagagc gtcgacttga agaagcgtaa attggaggtg acaagcaatg
     9181 aaaaataaag tagaacatat agaaaatcag tacacgtcgc aagagaataa gaaaaaacaa
     9241 cgtcaaaaaa tgaaaatgcg tgttgttcgt aggcgtatta cagtatttgc gggcgtatta
     9301 cttgcgataa ttgttgtttt atcaatcttg cttgttgtcc aaaaacatcg caatgatatc
     9361 gatgcacagg agcgaaaagc gaaagaagca cagtttcaaa agcaacaaaa tgaagaaatt
     9421 gcgttaaaag aaaagttgaa taatctgaat gacaaagatt atattgaaaa aattgcgcgt
     9481 gatgattatt acttaagcaa caaaggtgaa gtgattttta ggttgccaga agacaaagat
     9541 tcgtctagct caaaatcttc gaaaaaataa atccaaattg attcaaaatt atccgagtat
     9601 agacattgtg aaaaaatcca aacaaggata taataaggga aaatcgaatc aaatcgggag
     9661 gatttattta acatatgtca atcgaagttg gaaataagct taaaggtaaa gtcactggta
     9721 ttaaaaagtt tggtgcattc gtagaattac ctgaaggaaa aagtggttta gttcacatta
     9781 gtgaagtcgc agataattat gttgaaaacg tagaagagca cctttctgtt ggtgatgaag
     9841 tagacgtaaa agtattatct attgctgatg atggaaaaat tagtctttca attaagaaag
     9901 ctaaagaccg tccacgtaga caacatacga gtaaaccaag tcatcaaaaa ccagtgcaaa
     9961 aagccgaaga ttttgaaaag aaattaagca atttcttaaa agatagtgaa gataaattaa
    10021 cttcaatcaa acgtcaaaca gaatctagac gcggtggcaa aggttcaaga cgttaattaa
    10081 aataaataaa gactgtttcg ataaggaata tatttagaat gatgcgtatc gaataatcga
    10141 ttgcagcgtt agacaatcta agactgtttc ttaaataagg agcagtctct tttatttgta
    10201 atgatataac taagacttat accatttttg aaaattgtaa aagtgaggtg atgttatgca
    10261 gttaaatagt aatggttggc atgttgatga ccatattgtt gtcgctgttt ctacaggtat
    10321 tgatagtatg tgtttattgt atcaactact aaatgattat aaagatagtt atagaaaact
    10381 aacatgctta catgtcaatc atggcgttag gtcagcttca atcgaggaag ccagattttt
    10441 agaagtatac tgcgaacgtc atcacatcga tttacatatc aaaaagttag atttgtcgca
    10501 tagtctcgac cgaaataaca gcattcagaa tgaagctcga attaaacgtt acgaatggtt
    10561 tgatgaaatg atgaatgtat tagaagcgga tgtattgcta acggcgcatc atttggacga
    10621 tcaattagaa actattatgt atcgtatttt taatgggaaa tcaacgcgta ataaactagg
    10681 atttgatgag ttatcgaagc gaaaaggtta tcagatttat cgaccacttt tagctgtctc
    10741 taaaaaagaa ataaaacaat tccaagagag atatcatatt ccatattttg aagatgaatc
    10801 taataaagat aacaaatatg ttagaaatga tattcgtaat agaattattc cagctattga
    10861 tgaaaataat caacttaaag catcgcattt attaaaatta aaacaatggc atgatgaaca
    10921 atatgatatt ttgcaatatt cagctaaaca atttattcaa gaatttgtga agtttgatga
    10981 acagtcaaaa tatttagagg tttctagaca agcttttaat aacttaccaa actcattaaa
    11041 gatggttgtg ttggattgcc tactatcaaa gtattatgag ttgtttaata ttagtgctaa
    11101 aacatacgaa gagtggttta aacaatttag tagtaagaaa gcacaattca gtattaatct
    11161 cacggataaa tggataattc aaattgcata tggtaaatta ataataatgg ctaaaaataa
    11221 tggcgataca tattttagag ttcaaactat tgaaaagcca ggtaattata tttttaacaa
    11281 atatcgatta gagatacatt ctaatttacc aaaatgttta tttccgctta cagtgagaac
    11341 acgacaaagt ggcgatacat ttaaactgaa tgggcgcgat ggttataaga aagtgaatcg
    11401 cctgtttata gattgtaaag tgcaacagtg ggttcgggat caaatgccaa tcgtattgga
    11461 taaacaacag cgcattattg cggtaggaga tttatatcaa caacaaacaa taaaacaatg
    11521 gattataatt agtaaaaatg gagatgaata gcgttatgca taatgatttg aaagaagtat
    11581 tgttaactga agaagatatt caaaatatct gtaaggaatt gggagcacaa ttaacaaagg
    11641 attatcaagg taaaccatta gtatgcgtgg gtatcttaaa aggctcagca atgtttatgt
    11701 cagatttaat taaacgaatt gatacccatt tatcaattga tttcatggat gtttctagtt
    11761 atcacggagg cactgagtca actggtgaag ttcaaatcat taaagattta ggttcttcta
    11821 ttgaaaataa agacgtatta attattgaag atatcttaga gactggtact acacttaagt
    11881 caattactga attattacaa tctagaaaag ttaattcatt agaaatagtt actttattag
    11941 ataaaccaaa ccgtcgtaaa gcggacattg aagctaagta tgtaggtaaa aaaataccag
    12001 atgaatttgt tgttggttac ggtttagatt atcgtgaatt ataccgaaac ttaccatata
    12061 tcggtacgtt aaaacctgaa gtgtattcaa attaattttt taatcaattt cagttattat
    12121 tactatgcgt ttgagaaata atagtgtaga ctcaaaaata tgaaaaatgt atttcatata
    12181 tatttaattt tagacaagac atatgtcttg aaaagttgaa aaatatagag attgataaaa
    12241 ctaatacggg tgtgaatgac attgatgtta agctcaatta ctagcttata aaacatgtca
    12301 tatgttacaa tttttgttag ttttattatg ggaagtagga ggaaatgacg catgcagaaa
    12361 gcttttcgca atgtgctagt tatcgtaata ataggcgtta ttatttttgg tctattttca
    12421 tatttaaacg gtaatggaaa tatgccgaaa cagcttacat ataatcaatt tactgagaag
    12481 ttggaaaaag gtgaccttaa aactttagaa atccaaccac aacaaaatgt ctatatggta
    12541 agtggtaaaa cgaaaaatga tgaagactat tcatcaacta ttttatataa caacgaaaaa
    12601 gaattacaaa aaattactga tgctgctaaa aagcaaaacg gtgtaaaatt aacgattaaa
    12661 gaagaagaaa aacaaagtgt ctttgtgagt atactttcaa cattaattcc agttgtagtc
    12721 atagcgttat tatttatttt cttcctaagc caagcacaag gtggcggtag tggcggtcgt
    12781 atgatgaact ttggtaaatc taaagcaaaa atgtacgata ataataaacg tcgtgttcgt
    12841 ttctctgatg tagcaggggc agatgaagaa aaacaagaat taattgaaat tgttgatttc
    12901 ttgaaagata ataaaaaatt caaagaaatg ggatctagga ttcctaaagg tgtcttactt
    12961 gttggacctc caggtactgg taaaacatta cttgctagag cggttgcagg tgaagctggc
    13021 gcaccattct tctctattag tggttcagac tttgtagaga tgtttgttgg tgttggtgcg
    13081 agccgtgttc gtgacttatt cgataatgct aagaaaaacg cgccttgtat catctttatc
    13141 gatgagattg atgctgttgg tcgtcaacgt ggtgcaggtg ttggtggcgg tcatgatgaa
    13201 cgtgaacaaa ccctaaacca attattagtt gaaatggatg gtttcggtga aaatgaaggt
    13261 atcattatga tagctgctac aaaccgtcct gatatccttg acccagcctt attacgtcca
    13321 ggtcgttttg atagacaaat tcaagttggt cgtccagatg tgaaaggccg tgaagcaatt
    13381 cttcatgttc atgctaaaaa caaaccactt gatgaaacgg ttgatttaaa agcaatttca
    13441 caacgtacac ctggtttctc aggtgctgat ttagagaact tattaaatga agcatcttta
    13501 attgctgtac gtgaaggtaa aaagaaaatt gacatgagag atatcgaaga ggcaacggat
    13561 agagttatag ccggacctgc taagaaatct cgagttattt ctaagaaaga acgtaatatt
    13621 gttgctcatc acgaagctgg tcatacaatt atcggtatgg tacttgatga ggcagaagta
    13681 gtgcataaag ttactattgt tccacgtgga caagcaggtg gttatgcaat gatgctacct
    13741 aaacaagatc gtttcttaat gactgaacaa gagttattag ataaaatctg tggtttactt
    13801 ggtggacgtg tatcagaaga tattaacttt aacgaagtat caacaggtgc ttcaaatgac
    13861 ttcgaacgtg caacacaaat cgcacgctca atggttacgc aatatggtat gagtaaaaaa
    13921 ttaggaccat tacagttcgg tcatagcaat ggtcaagtat tcttaggtaa agatatgcaa
    13981 ggtgagccta attattcaag ccaaatcgca tatgaaattg ataaagaagt tcaacgaatc
    14041 gttaaagaac aatacgaacg ttgtaaacaa attttattag agcacaaaga acaattaatt
    14101 ttaattgctg aaacattatt aacagaagaa acattagttg ctgaacaaat tcaatcatta
    14161 ttctacgaag gtaaattacc tgaaattgat tatgatgcag ctaaagttgt taaagatgaa
    14221 gattctgaat ttaatgatgg taaattcggt aaatcttatg aagagattcg taaagagcaa
    14281 ttagaagatg gacaacgtga cgaaagtgaa gatcgtaaag aagaaaaaga tattgctgag
    14341 gataaaaaag aagctgataa atctgatgaa aaagatgaac cagcacatcg acaagcccca
    14401 aatatcgaaa aaccttacga tccaaatcac ccagacaata aataatcgat tatattcagt
    14461 acctctttct atgataaagt tatagaaaga ggtactttta tcgtttttga aaatacgtat
    14521 tagattttaa gtcgttgaat tgttatagca gaaaataatt gtaaaacaag ttacttcatt
    14581 atttagaatg atgggtgtag aataagtaca attgttgcat tttatgaagt aaagtaattt
    14641 tttaaatata gagtaataga ggagattgaa ataatgacac acgattatat tgttaaagca
    14701 ttagcatttg atggagagat tagggcttat gctgctttga caactgaaac tgttcaagaa
    14761 gcacaaacga gacattatac atggccgaca gcatctgctg caatgggaag aacaatgaca
    14821 gcaacagcta tgatgggcgc aatgttgaaa ggtgatcaaa aattaactgt cactgtagat
    14881 ggccaaggac ctattggacg aattattgcc gatgcaaatg ctaaaggcga ggtgcgtgct
    14941 tatgtagacc atccacaaac tcattttcca ttaaatgagc aaggtaaact tgatgtaaga
    15001 cgagcagtag ggacaaatgg atctattatg gttgttaaag acgttggaat gaaagactat
    15061 ttctctggag caagtccaat tgtttcagga gaacttggtg aagattttac ttattattat
    15121 gctacaagtg aacaaacacc ttcatcggta ggtcttggtg tattggtaaa tcctgataat
    15181 acgattaaag cagcaggagg atttatcatt caagttatgc caggtgccaa agatgaaaca
    15241 atttcaaaat tagaaaaagc aattagtgaa atgacaccag tttctaaatt aattgaacaa
    15301 ggattaacgc cagaaggatt actaaacgaa atcttaggtg aagaccatgt gcaaatttta
    15361 gagaaaatgc ctgttcaatt tgaatgtaat tgtagtcatg agaaattttt aaatgctatt
    15421 aaaggattgg gcgaggctga gattcaaaat atgattaaag aagatcatgg tgctgaagca
    15481 gtatgtcatt tctgtggaaa taaatataaa tatactgaag aagaattaaa cgtgttgcta
    15541 gaaagtttag cgtaatttaa tttaaatcaa tacgctaaaa tgtttatttt tagcggttta
    15601 gtgaaatgta gaactaaata gttgtataat ccttagtgat tttgtttgct ttctagaatt
    15661 tatttgataa aataattcta tatccgataa ataaactaag atttcaacaa ctaactaaaa
    15721 aggagtgttc ttaatggcac aaaaaccagt agataatatt actcaaatta ttggcggtac
    15781 accggtagtc aaattgagaa atgtagtaga tgacaatgca gcagatgttt atgtaaaatt
    15841 ggaatatcaa aatccaggtg gttctgtaaa ggatagaatt gctttagcaa tgattgaaaa
    15901 agcagagcga gaaggcaaaa ttaaacctgg cgatacaatt gtagaaccaa caagtggtaa
    15961 tacaggtatc ggtttagcat ttgtatgtgc tgctaaagga tataaagcag tatttactat
    16021 gcccgaaaca atgagccaag agcgtcgtaa tttattaaaa gcatacggtg cggaattagt
    16081 tttaacgcct ggatcagaag cgatgaaagg tgcaattaaa aaagctaaag aattgaaaga
    16141 agaacatggt tacttcgagc cacaacaatt tgaaaaccct gcgaaccctg aagttcatga
    16201 gttaactaca ggtcctgagt tattacaaca atttgaaggg aaaactatcg atgcgttcct
    16261 agctggtgtt ggtactggtg gtacgttatc tggtgtaggt aaagttctga aaaaagaata
    16321 tcctaacatc gaaattgttg ctatagagcc tgaggcttct ccagtattga gcggtggtga
    16381 gccaggtcca cataaattac aaggtttagg tgctggattt attccaggca ctttgaatac
    16441 agaaatctat gacagtatta ttaaagtagg aaatgataca gcgatggaaa tgtctcgtcg
    16501 agttgctaaa gaggaaggta ttttagcagg tatttcatca ggtgctgcga tttatgctgc
    16561 cattcaaaaa gcaaaagaat taggaaaagg taaaacagta gtaacagtat tgccgagtaa
    16621 tggtgaacgc tacttatcaa cacctttata ttcattcgat gactaattaa tgtcatttaa
    16681 aagagtgagt tatctttttg agataacttg ctcttttttt ctaccatgta tatttttaaa
    16741 aatatgagcg ttaaattaaa catttttctg ataaaaatat ccagtgaatg ataagataat
    16801 aaacgtacat actaataact agtaaatagc aggagtaaat tttattagag ttaaacaata
    16861 cataattaaa gggtggttaa catgactaaa acaaaaatta tgggcatatt aaacgtcaca
    16921 cctgattcat tctcagatgg tggaaaattt aataatgttg aatcagctat aaatagagtg
    16981 aaagccatga tagatgaagg tgttgacatt atagatgttg gaggtgtttc aacgagacca
    17041 ggtcatgaaa tggtttcatt agaagaagag atgaacagag tattacctgt tgttgaagct
    17101 attgtcggtt ttgatgtaaa aatttcagtc gatacatttc gaagtgaggt tgctgaagca
    17161 tgtttaaaat taggcgttga tatgattaat gatcaatggg cgggtctgta tgatcatcgt
    17221 atgttccaaa ttgtagctaa atatgacgcg gaaattattt taatgcataa tggaaatggt
    17281 aatcgtgatg aaccggttgt cgaagaaatg ttaacatctt tgttagcaca agcacatcaa
    17341 gctaaaatag ctggtatacc ttcaaataaa atttggctag atccaggtat aggtttcgct
    17401 aaaactagaa atgaagaagc cgaagttatg gcaagactgg atgaacttgt tgcaacagaa
    17461 tatccagttt tattagcgac aagccggaaa cgtttcacta aagagatgat gggttatgat
    17521 acaacaccgg ttgaaagaga tgaagtaact gcagctacga ctgcatatgg tattatgaaa
    17581 ggcgttagag cagtacgcgt tcataatgtc gagttgaatg ctaaattagc taaaggtata
    17641 gattttttaa aggagaatga aaatgcaaga cacaatcttt cttaaaggta tgcgctttta
    17701 tggatatcat ggtgctttat cagctgaaaa tgaaataggg caaattttca aagtggatgt
    17761 aactttgaaa gtagacttag ctgaagctgg gcgtactgat aatgttattg atacagttca
    17821 ttatggtgaa gtgttcgaag aggttaaatc aattatggaa ggtaaggccg ttaatttact
    17881 tgagcatcta gctgaacgta ttgcaaatcg tataaattca caatataatc gtgtaatgga
    17941 aacgaaagtg agaatcacta aagaaaaccc accgattccg ggtcattatg atggagtagg
    18001 tatcgaaata gtgagggaga ataaatgatt caagcatact taggattagg tagtaatatt
    18061 ggtgatagag aaagccagtt aaacgatgct ataaagattt tgaatgaata tgatggtatt
    18121 aacgtatcta atatttctcc gatttatgaa acagcaccag ttgggtatac tgagcaacct
    18181 aactttttaa atttgtgtgt tgaaattcaa acaacactca cagtattaca actgttggaa
    18241 tgttgtttga agacagaaga atgtttacac cgtattagaa aggaacgatg gggtcctaga
    18301 actttagatg tggatatttt gttgtatgga gaagaaatga tagatttacc aaaactgtcg
    18361 gtgccacatc cgagaatgaa tgaacgtgca tttgttttaa tcccattaaa tgatatagca
    18421 gcaaatgtcg tagaaccacg ttcgaaattg aaagtgaaag atttagtttt tgtagatgac
    18481 agtttaaaga gatataaata atgcattgtt gagaacattc atatttattg ggaatagatg
    18541 attgattatt ggagatgttt ccgcattttg attatctcta aatgcatttg atttcgaatt
    18601 agtatatgat gatttagtat gtgttagaat taagttattt cataaaatca aagtaagcaa
    18661 tatttgataa attgctgtaa tttatttggg cttttgttga agtttgtgga agataatttt
    18721 tatacattta cttagggttg tattaatgat aatcgtattt taaatgaata agtaattcat
    18781 aaacgaaaac agaaattgct ttattacatt tacaagctct tgtaaaatta aaaaagtgat
    18841 aaaattgatg ttatgaatat aagaaaacat gccacatcat aactttaagg tgtaatggtt
    18901 aatgataaag tattagaaac atcgaaagag atgtatgttg agcaaaaatg tctgatattt
    18961 tataaaactt taaaggaaaa tgtttgagtg taccagttgg aatactaaag gattacaaca
    19021 agttaaagga gagaaagtta tgtcagaaga aatgaatgac caaatgttgg ttcgacgtca
    19081 aaaattacaa gaattatatg atcttggtat agacccgttt ggttctaaat ttgaccgttc
    19141 aggtttatct agtgatttga aagaagagtg ggaccagtat tctaaagaag aattggtaga
    19201 aaaagaagcg gatagtcatg tcgctatagc tggacgatta atgactaagc gtggtaaagg
    19261 taaagcagga tttgcacacg ttcaggactt agctggacaa attcaaattt acgttcgtaa
    19321 agatcaagtt ggcgatgacg aatttgattt atggaaaaat gctgatttag gcgatatcgt
    19381 tggtgttgaa ggtgtaatgt tcaaaacaaa tactggcgaa ttatcggtta aagcgaagaa
    19441 attcacgcta ctaactaaat cattgcgacc attaccggat aaattccacg gtttacagga
    19501 tattgaacag agatatcgtc aaagatattt agatttaatt acgaacgaag atagcactcg
    19561 tacatttatt aatcgtagta aaatcattca agaaatgcgt aattatttaa ataataaagg
    19621 tttcttggaa gtagaaacac ctatgatgca ccaaattgct ggtggagcag ctgctagacc
    19681 atttgtaaca catcataatg cattagatgc aacgttatac atgcgtattg ctattgagtt
    19741 gcatttaaaa cgtttaattg tcggtggact tgaaaaagta tatgaaattg gtagagtatt
    19801 ccgtaatgaa ggtgtatcaa ctagacataa ccctgaattc acaatgattg aattatatga
    19861 agcatatgca gattatcatg acattatgga tttaacagaa tctatggtga gacatattgc
    19921 caatgaagtg ttaggttctg caaaagtaca atacaatggg gaaacgattg atttagaatc
    19981 tgcttggact cgtttgcata ttgttgatgc tgtaaaagaa gctactggtg tagattttta
    20041 tgaagttaaa agtgatgatg aagctaaagc tttagctaaa gaacatggta ttgaaattaa
    20101 agatacaatg aaatatggtc atattttaaa tgaattcttt gagcaaaaag ttgaagaaac
    20161 acttattcag ccaacgttta tctatggtca tccgactgaa atttcacctt tagcgaagaa
    20221 aaatcctgaa gatcctagat ttactgatcg tttcgaattg ttcattgtag gtagagagca
    20281 tgcaaatgca tttactgaat taaatgatcc tattgatcaa aaaggtcgtt ttgaagcgca
    20341 acttgttgaa aaagcgcaag gtaatgatga agcgcatgaa atggatgaag attacattga
    20401 agcgttagaa tatggtatgc ctccgacagg tggtcttggt atcggtattg acagattggt
    20461 tatgttatta actgactctc catcaatcag agacgtatta ttattccctt atatgagaca
    20521 aaaataaatg acgttgattg ttagtaagag ctctcgtgta tacaacatgt gtatgcgagg
    20581 gctttcttaa ttatggtaat tagttcgtgt ttgaatgttt ttgatagtaa tagttaacga
    20641 tagtggtgct atttttgact gttaaacaag gtagttggct gatagatgaa aatgacgata
    20701 cgtatataga gtagttcgga tgagaaatgt taacgataga cattgagata tcttataaca
    20761 aaaattgcta tattagtata atttatctta atcagctata aaagtacttt aaaattgtat
    20821 agaatgtgta tggttttgta catatgtgta tgatagaata ctaaaagtgt tatgaattag
    20881 agggcacgag aaatgtcagt tttgaagaat aaaaaagttg attaaaagtg ttgactttat
    20941 caattgaatg aagtaatata taaaagtcgt caaaaacaga cgaaacacac taaaagctga
    21001 tgtgacaaag tttacatcaa agtgtaaaat taactattgc accttattaa ttaagcgtgt
    21061 atcatgaata agtaagttat tttgtctggt gactatagca aggaggtcac acctgttccc
    21121 atgccgaaca cagaagttaa gctccttagc gtcgatggta gtcgaactta cgttccgcta
    21181 gagtagaacg ttgccaggca aatgacaaat cggagaatta gctcagctgg gagagcatct
    21241 gccttacaag cagagggtcg gcggttcgaa cccgtcattc tccaccattt attcttagat
    21301 atagccggcc tagctcaatt ggtagagcaa ctgacttgta atcagtaggt tgggggttca
    21361 agtcctctgg ccggcaccat cttttgagcc attagctcag ctggtagagc atctgacttt
    21421 taatcagagg gtcagaggtt cgaatcctct atggctcatt acgatttaat ttttatattt
    21481 agcaaaataa tgcagaagta gttcagcggt agaatacaac cttgccaagg ttggggtcgc
    21541 gggttcgaat cccgtcttct gctccattat tttgccgggg tggcggaact ggcagacgca
    21601 caggacttaa aatcctgcgg tgagagatca ccgtaccggt tcgattccgg tcctcggcac
    21661 cattttagcg cccgtagctc aattggatag agcgtttgac tacggatcaa gaggttatgg
    21721 gttcgactcc tatcgggcgc gccattttta aattaattga ataacgggaa gtagctcagc
    21781 ttggtagagc acttggtttg ggaccaaggg gtcgcaggtt cgaatcctgt cttcccgatt
    21841 acttcttaaa ttccatttta tgagggctta gctcagctgg gagagcgcct gctttgcacg
    21901 caggaggtca gcggttcgat cccgctagtc tccaccattt attttttaca cgatgaacat
    21961 tgaaaactga atgacaatat gtcaacgtta attcc
//
LOCUS       DAMZKC010000028        21882 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.028, whole genome shotgun sequence.
ACCESSION   DAMZKC010000028 DAMZKC010000000
VERSION     DAMZKC010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 21882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21882
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.028"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(368..1597)
                     /locus_tag="POB55_000810"
     CDS             complement(368..1597)
                     /locus_tag="POB55_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HDI6499785.1"
                     /translation="MGRNLKLKKESDFEFTKNHKRLLLGSVFLMATSAIGPAFLTQTA
                     VFTSQFFASFAFAILLSIIIDIGAQINIWRILVVTGLRGQEISNKVVPGLGTVISILI
                     AFGGLAFNIGNIAGAGLGLNAIFGLDVKWGAAITAIFAILIFVSKSGQKIMDVVSMIL
                     GIVMILVVAYVMFVSNPPYGDAFVHTFAPEHPMKLVLPIITLVGGTVGGYITFAGAHR
                     ILDSGIKGKQYLPFVNQSAIAGILTTGIMRTLLFLAVLGVVVTGVTLSSENPPASVFE
                     HAIGPIGKNIFGIVLFAAAMSSVIGSAYTSATFLKTLHKSLNERSNLIVIVFIVISTM
                     IFLFIGKPISLLIIAGAINGWILPITLGAILIASKKKSIVGDYKHPNWMFIFGIVAVL
                     VTILTGIFSFKEVLQLF"
     gene            complement(1609..2361)
                     /gene="pxpA"
                     /locus_tag="POB55_000811"
     CDS             complement(1609..2361)
                     /gene="pxpA"
                     /locus_tag="POB55_000811"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HDI6499786.1"
                     /translation="MRVDLNCDLGEAFGNYSFGGDHQIIPLITSANVACGFHAGDENV
                     MNETVKLAKAHNVAVGAHPGLPDLKGFGRRNIDISNDEIYNLMIYQLGALQGFCRIHQ
                     VKINHVKPHGALYQMGAKDREIASVIAQAVYDFDPSLVLVGLANSYLISEAKNVGLIT
                     ASEVFADRRYEDDGQLVSRKESDAVITDTDEALKQVLKMVKENKVISKNNKEVTLQAD
                     TICVHGDGEHALLFVSKIREILMKEGIDIQSL"
     gene            complement(2361..3722)
                     /locus_tag="POB55_000812"
     CDS             complement(2361..3722)
                     /locus_tag="POB55_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDI6499787.1"
                     /translation="MLRCLIANRGEIAVRIIRACREYGIETVAVYAKGDEQSLHVHLA
                     DQAICIGEANALGSYLNIDRIISAAQITGANAIHPGYGFLSESTKFAQTVEEQGIAFI
                     GPTKKTMEMMGDKITARQTVHHAGVPVIPGSNGAVNHVSEIEKLAKDIGYPVVIKAAS
                     GGGGKGIRIVKKAEDLEKAFKEAKSEGKKYFDDDRVYVEAFIPVAKHVEVQVMGDGQD
                     NFVHLGERDCSVQRKNQKLIEESPCAALTEERRQQICNDAVKVARAANYRSAGTIEFL
                     VTDTAHYFIEMNARIQVEHTVTEMRAERDLVAAQLYLLEHNHLPFSQSDIQFNGHVIE
                     ARINAENPEKKFQPTPGKVTALHLPQGFNVRVDSLLYHGYQVSPYYDSLVAKVIVKSH
                     DRASAIDKLKATLDEMVIDGFSTTADFLYAVLNYPLYRDGDAKDVDIKFLEKHQIVKG
                     VEL"
     gene            complement(3736..4185)
                     /locus_tag="POB55_000813"
     CDS             complement(3736..4185)
                     /locus_tag="POB55_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HDI6499788.1"
                     /translation="MNIEKIEQIIKLVKENDVKKFKYKNFEDEIEIDFTDSNHLAAHS
                     NQSNQSMNNNDLTASKANDNSDVSTNDYHDIKSPMVGTFFLQDSKELTEPIVNVGDKV
                     NKGDIIGYVEAMKVLNEVTTDVAGEITEIVADHGTNVEYDQVLVRIK"
     gene            complement(4187..5197)
                     /locus_tag="POB55_000814"
     CDS             complement(4187..5197)
                     /locus_tag="POB55_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HDI6499789.1"
                     /translation="MSIKILQPGLFSTVQDLGRKGYEHIGFSGAGAMDQFSFKVAQLL
                     INNDGPAIEYTLIGPTIQFNTQNTFVITGGSVNASLNNKTISMNSVILAEKGDILKIG
                     AITKGARGYLTFGHSINVPPIAESYATHTRSSIGGFKGRKLLANDVITVKINNDFKEN
                     IGKTIHLQDDLLPENNIIHILQGPQFEAFSEEARAKIVNHPYLITEQSDRMGYRLEGD
                     SVAPINQADIISEPVALGSVQVPNDGQPIILLNDKQTIGGYTKIATVCKFDLPKLAQM
                     KPQDTIQFKWISFQEAVDKNREQMSLFNEILKSHQKTPIFDTSSLRHTSKKLATILKG
                     DL"
     gene            complement(5187..5921)
                     /gene="pxpB"
                     /locus_tag="POB55_000815"
     CDS             complement(5187..5921)
                     /gene="pxpB"
                     /locus_tag="POB55_000815"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HDI6499790.1"
                     /translation="MDVRFINEQTIMIYFENKISEETYRNVTAMVRWIREKEILEIQD
                     IVPSYRAVLIYFDEQAITSSKLIENLELNKFNEKNVHAVNQTNRIIKIPVQYGGTYGP
                     DIEEVAKHNRITVEQVIEKHTSKPYLIYMLGFMPGFPYLGGLDEQLHTPRRNQPRLKI
                     HAGSVGIANNQTGLYPSDSPGGWQIIGRTPLKVFSSEREPMSMYEAGEWIQFYAIDEQ
                     EFIQIERDISDGNFNVDDWVVIENVN"
     gene            complement(6247..6723)
                     /gene="greA"
                     /locus_tag="POB55_000816"
     CDS             complement(6247..6723)
                     /gene="greA"
                     /locus_tag="POB55_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HDI6499791.1"
                     /translation="MENQKQYPMTQEGFEKLERELEELKTVKRPEVVEKIKVARSFGD
                     LSENSEYDAAKDEQGFIEQDIQRIEHMLRNALIIEDTGDNNVVKIGKTVTFVELPGDE
                     EESYQIVGSAESDAFNGKISNESPMAKALIGKGLDDEVRVPLPNGGEMNVKIVNIQ"
     gene            complement(6751..7374)
                     /gene="udk"
                     /locus_tag="POB55_000817"
     CDS             complement(6751..7374)
                     /gene="udk"
                     /locus_tag="POB55_000817"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HDI6499792.1"
                     /translation="MKATTIIGIAGGSGSGKTTVTNEIMKNLEGHSVALLAQDYYYKD
                     QKHLTFDERLETNYDHPFAFDNDLLIENLKDLKNGKAVEVPTYDYASHTRSDITIDFK
                     PKDVIIVEGIFALENKVLRDMMDVKIYVDTDADLRILRRLTRDTKERGRSMDSVINQY
                     LSVVRPMHDQFIEPTKKYADIIIPEGGSNKVAIDIMTTKIQSLVSKQ"
     gene            complement(7374..8642)
                     /locus_tag="POB55_000818"
     CDS             complement(7374..8642)
                     /locus_tag="POB55_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDI6499793.1"
                     /translation="MKTIEEIKSTPKTVMKKPELLAPAGNLEKLKIAVHYGADAVFLG
                     GQEYGLRSNADNFTMEEIAEGVEFANRYGAKIYVTTNIIAHDENIEGLESYLRNLEKT
                     GATGIIVADPLIIETCKEVAPKLEIHLSTQQSLSNYKAVEYWKEEGLDRVVLARETGA
                     MEMREMKEKVDIEIEAFIHGAMCIAYSGRCTLSNHMTARDSNRGGCCQSCRWDYELLE
                     VDDNGELDVFYNQGEVTPFAMSPKDLKLIESIPQMMDIGVDSLKIEGRMKSIHYIATV
                     VSVYRKVIDAYAADPDNFKINPEWLIELDKCANRDTAPAFFEGTPGYEEQMFGQQQSK
                     KSPFDFCGLVLDYNEDTKIATIQQRNNFKPGQEIEFFGPEIETFTQVVEAIYDEEGNS
                     LDAARHPLQIVQIKVDRPIYPNNMMRKEIG"
     gene            complement(8654..9577)
                     /locus_tag="POB55_000819"
     CDS             complement(8654..9577)
                     /locus_tag="POB55_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDI6499794.1"
                     /translation="MTELLVTPKSLSHMETLIDLGADAFVIGEQKFGLRLPGEFNRQQ
                     MTEAVALAHKNDKKVYAAVNGLFHNYHLDAVEDYINFLHEIRVDRIIFGDPAVVMYVK
                     AQENPIPLHWNAETLVTNHFQCNYWGKRGASRAVLARELNLDEIINIKENSNVEIEVQ
                     VQGMTCMFQSKRMLLGNYYTFQDRQMKIERRNDEQSLLLYDEERQNNYPVYEDYNGTH
                     IMSPNDICLIEELAPFFEAGIDSFKIDGILQTEEYINVVTEQYRQAIDLYNEDPEIYE
                     DEKFMLMDPIEEIQPDHRPFDEGFLYKQTVY"
     gene            complement(9580..10218)
                     /locus_tag="POB55_000820"
     CDS             complement(9580..10218)
                     /locus_tag="POB55_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HDI6499795.1"
                     /translation="MDDLNKKYLIDLHQHQNSSIEVLREFAEVNEVPIVDRLTLDLIK
                     QLIRMNNVKNILEIGTAIGYSSMQFASISDDIHVTTIERNETMIQYAKQNLATYHFEN
                     QVRIIEGNALEQFENVNDKVYDMIFIDAAKAQSKKFFEIYTPLLKHQGLVITDNVLYH
                     GFVSDIGIVRSRNVRQMVKKVQDYNEWLIKQPGYTTNFLNIDDGLAISIKGE"
     gene            complement(10503..10811)
                     /locus_tag="POB55_000821"
     CDS             complement(10503..10811)
                     /locus_tag="POB55_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HDI6499796.1"
                     /translation="MTEHNHDSQLEINNEEELLTLFDEEGNEVLYRKVLEFYHPEFKK
                     EYVILAEEGAQSDEDDMIELVPMINEPDESGDGGKLVPIETDEEWDMIEEVVNTEMEE
                     "
     gene            complement(10826..11254)
                     /gene="ruvX"
                     /locus_tag="POB55_000822"
     CDS             complement(10826..11254)
                     /gene="ruvX"
                     /locus_tag="POB55_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HDI6499797.1"
                     /translation="MLQHKILGLDVGSRTVGIAISDIMGWTAQGLDTLRINEENNELG
                     IDQLVDIIKKHNVGTVVIGLPKNMNNSIGFRGEASLTYKEKLLEAYPSIEIVMWDERL
                     STMAAERSLLEADVSRQKRKQVIDKMAAVFILQGYLDSLH"
     gene            complement(11258..11518)
                     /locus_tag="POB55_000823"
     CDS             complement(11258..11518)
                     /locus_tag="POB55_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HDI6499798.1"
                     /translation="MENFDKTMKFDYEELPTQDVRDVLNNVYRTLDERGYNAVNQIVG
                     YLLSGDPAYIPRQNEARNQIRHIDRDVIMEELVSYYLKEQNK"
     gene            complement(11581..14211)
                     /gene="alaS"
                     /locus_tag="POB55_000824"
     CDS             complement(11581..14211)
                     /gene="alaS"
                     /locus_tag="POB55_000824"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HDI6499799.1"
                     /translation="MKKLKASEIRQKYLDFFVEKGHMVEPSAPLVPIDDDTLLWINSG
                     VATLKKYFDGRETPKKPRIVNSQKAIRTNDIENVGFTARHHTFFEMLGNFSIGDYFKQ
                     EAIEFAWEFLTSDKWMGMEPDKLYVTIHPEDMEAYNIWHKDIGLEESRIIRIEGNFWD
                     IGEGPSGPNTEIFYDRGEAYGQDDPAEEMYPGGENERYLEVWNLVFSEFNHNKDHSYT
                     PLPNKNIDTGMGLERMASVSQNVRTNYETDLFMPIMNEIEKVSGKQYLVNNEQDVAFK
                     VIADHIRTIAFAISDGALPANEGRGYVLRRLLRRAVRFSQTLGINEPFMYKLVDIVAD
                     IMEPYYPNVKEKADFIKRVIKSEEERFHETLEDGLAILNELIKKAKATTNEINGKDAF
                     KLYDTYGFPIELTEEIAVQAGLKVDMTTFESEMQQQRDRARQARQNSQSMQVQSEVLK
                     NITSASTFVGYDTATAQTTLTHLIYNGEEVSQVEAGETVYFMLTETPFYAVSGGQVAD
                     TGIVYNDNFEIAVSEVTKAPNGQNLHKGVVQFGQVNVGATVSAEVNQNDRRDIQKNHS
                     ATHLLHAALKSVLGDHVNQAGSLVEADRLRFDFSHFGPMTNDEIDQVERLVNEEIWKG
                     IDVNIQEMDIASAKEMGAMALFGEKYGDVVRVVNMAPFSIELCGGIHVRNTSEIGLFK
                     IVSESGTGAGVRRIEALTGKAAFLYLEDIQEKFNTMKSQLKVKSDDQVVDKLTQLQDE
                     EKALLKQLEQRDKEITSLKMGNIENQVEEINGYKVLVTEVDVPNAKAIRSTMDDFKSK
                     LQDTIIILASNVDDKVSMVATVPKSLTNNVKAGDLIKQMAPIVGGKGGGRPDMAQGGG
                     TQPENISKSLSFIKDYIKNL"
     gene            complement(14554..17031)
                     /locus_tag="POB55_000825"
     CDS             complement(14554..17031)
                     /locus_tag="POB55_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HDI6499800.1"
                     /translation="MSDPTLFDYSMIKGTVEAILFQNSDNFYTVLKVDTIETNEDFDT
                     MPTVVGFLPNIVEGDVYTFKGQVVDHPRYGKQLKAETFEKEMPQTKEAIISYLSSDLF
                     KGVGKKTAQNIVNTLGDNAINDILDDHSVLEKVSGLSKKKQKQIAEQISANQESEKIM
                     IRLHDLGFGPKLSMAIYQFYLGDTLTILDRNPYQLIYDIKGIGFNKADQLARNIGIAY
                     NDNERLKAALLYTLEEECIKQGHTYLPINVVIDLTVDVLNYQDEEVIEPEKLDEMLQY
                     LNEEKRLIIDNEQVAIPSLYYSEIKSVQNLFRIKTHTNKLTEIEQSDLQMHIGEIEDA
                     NQVNYAASQKEALQTAINSKVMLLTGGPGTGKTTVIKGIVELYAEIHGLSLDYDDYVN
                     DDYPVVLAAPTGRASKRLQESTGLEAMTIHRLIGWNQDTKPEDILENEINARLIIIDE
                     MSMVDTWLFHQFLSAVPLDAQLIFVGDEDQLPSVGPGQVFKDLIESKAIPRVNLTEVY
                     RQQDGSSIIELAHRMKLGQKIDITQRFHDRSFINCQANQIPTVVEKVVTSAVNKGYTM
                     ADIQVLAPMYKGNAGIKRLNQVLQDILNPKKKDTREIEFGDVVFRKGDKVLQLVNRPN
                     DNIFNGDIGVIVGIFWAKENALNKDVLVVDFEGNEITFTKQDMMELTHAYCTSIHKSQ
                     GSEFPIVIMPIVKQYFRMLQRPILYTGLTRAKTSLVLLGDPEAFDIGLKTNGQARLTQ
                     LCTLLKNYFNSEDEEMLENTATNDTGASQTTIDDQVEAPMSSNDSGEVTSDSTKTDIN
                     VLTEATMFKIDPMINMGEITPYDFIER"
     gene            complement(17033..17701)
                     /locus_tag="POB55_000826"
     CDS             complement(17033..17701)
                     /locus_tag="POB55_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDI6499801.1"
                     /translation="MIDQQTIYQYIQNGKIEEALQALFGNIEENPTIIENYINAGIVL
                     ADANEIEKAERFFQKALTIDPKNGVVFYNLANVYYNQQRYQEAIKLYQQALQTEIEQV
                     DCNYMIGMAFNQLESFKLALPYLMTAAELDKDKDAEVQFQYGLVLCQLEMFNEAITQL
                     KHVLTIDKNHVDARYNLGLALFMKNEDIDEAITHFKEAVTIDPKHLLSQHALKTFTKM
                     KEEE"
     gene            complement(18282..19400)
                     /gene="mnmA"
                     /locus_tag="POB55_000827"
     CDS             complement(18282..19400)
                     /gene="mnmA"
                     /locus_tag="POB55_000827"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HDI6499802.1"
                     /translation="MSNKDIRVVVGMSGGVDSSVTAHVLKEQGYDVIGIFMKNWDDTD
                     ENGVCTATEDYNDVIEVCNQIGIPYYAVNFEKEYWDKVFTYFLDEYKKGRTPNPDVMC
                     NKEIKFKAFLDHAMNLGADYVATGHYARIHRHEDGHVEMLRGVDNNKDQTYFLNQLSQ
                     QQLSKVMFPIGDIEKIEVRRIAEEQGLVTAKKKDSTGICFIGEKNFKTFLSQYLPAQP
                     GDMITLDGKKMGKHSGLMYYTIGQRHGLGIGGDGDPWFVVGKNLKDNVLYVEQGFHHD
                     ALYSDYLIASDYSFVNPEDNDLDQGFECTAKFRYRQKDTKVFVKRENDHALRVTFAEP
                     VRAITPGQAVVFYQGDVCLGGATIDDVFKNEGQLNYVV"
     gene            complement(19401..20543)
                     /locus_tag="POB55_000828"
     CDS             complement(19401..20543)
                     /locus_tag="POB55_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDI6499803.1"
                     /translation="MEIYADYAATTPVKPEVVDAMMMIYNSHYGNPSSIHAKGRDARK
                     YLDESRRQIAQLLGADTHEIIFTSGATESNNTAIKGIVKANEQLGNHIITSKIEHHSV
                     LHVFEQLEREGFDVTYLDVDDTGAIDLDQLEETITDKTILVSIMFVNNEVGTVQQIYD
                     IQDIIAETNAYFHVDAVQAIGHLDVKFDEFEIDAMSITAHKFGGPKGVGALLVKDHVT
                     LDYPQLGGEQELKRRAGTENLAQIVGMAKALQLAEKNRDDNNIHLMNLKEQFLVKLQE
                     RAIPFELNGSMTDATGHIVNLYFPFVEVETMLTLLDMAQIYVSSGSACTAGSTQPSHV
                     LDAMFEDEERSNHSIRFSFNELTTENEINAIVAEIHKIYFKFKEES"
     gene            20855..21868
                     /locus_tag="POB55_000829"
     CDS             20855..21868
                     /locus_tag="POB55_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HDI6499804.1"
                     /translation="MAIKYSALNLVPIREGEDERTAINDMVKLAQHLDELSYERYWIA
                     EHHNAPNLVSSATALLIQHTLEHTKHIRVGSGGIMLPNHAPLIVAEQFGTMATLFPNR
                     VDLGLGRAPGTDMMTASALRRDQHDGVYKFPEEVSLLQQYFGPAHQQAYVRAYPAVGK
                     NVPLYILGSSTDSAHLAARKGLPYVFAGHFAPQQMKEAIEIYKTLFEPSDVLDEPYVI
                     VCLNTIVAENDDEAQYLASSMAQVMVSITRGRMQPVQPPTHELQNILTPREYAMAMER
                     QKISLIGSENTVQQKIQDFMETYGEVNEIMAISYIYDKDMQLDSYRRFKNVINQINEK
                     NTL"
BASE COUNT         6687 a         4285 c         3042 g         7868 t
ORIGIN      
        1 tgagcctaca attttgatta tgtatcacgt tcataatact ttccaatttg aaataccctg
       61 ttcgcaatta ttagttgagt tatatcaacc aatacagtgt aaacataaaa attccaaacc
      121 gtttaacagt atatcagaaa aaatgataat catttctttt atttttatta aatttcataa
      181 tgattcatat aatattcgca ttaaaacatc acaatactga tacacaacaa aaagagcagt
      241 acatttgtca tgcaccactc tcaacaaata tatctaatta tgctttataa atgaatcacc
      301 ggaaatcaaa atacatcttg cctaatacta acaacattta cagtgatgct attagtcaaa
      361 atgtctttta aaataactga agtacttctt tgaatgagaa tatacctgtc aatattgtaa
      421 caagtactgc aacgatacca aagatgaaca tccaatttgg gtgtttataa tcaccaacaa
      481 ttgatttctt tttacttgca atcaaaattg cacctaacgt aataggtaaa atccatccat
      541 ttatcgcgcc tgctataatt aaaaggctga ttggttttcc aataaataag aaaatcattg
      601 ttgaaataac gataaacaca atcacaatta aattacttct ttcgttaagt gatttatgaa
      661 gtgtttttaa aaatgttgcg cttgtgtatg ctgagccaat tactgatgac atagctgcag
      721 caaataacac aataccaaaa atattctttc caattggtcc aattgcgtgt tcaaaaactg
      781 acgctggtgg attttcagaa cttagtgtca cacctgttac aacaacacct aataccgcta
      841 ggaatagtaa cgttctcata atacctgtag ttaaaatacc agcaattgct gattgattta
      901 caaatggtaa atattgctta cctttaatgc cagagtctaa tatacgatgt gcacctgcaa
      961 aggtaatata accacctaca gttccaccaa ctaacgtaat gattggcaag actaatttca
     1021 ttggatgttc tggcgcaaat gtatgcacaa aagcatcacc ataaggtgga ttagaaacaa
     1081 acatcacata tgccacaact aaaatcatca caataccaag aatcattgaa acaacgtcca
     1141 taattttttg gccactttta cttacaaaga ttaatattgc aaagattgca gtaatagctg
     1201 cgccccattt tacatctaat ccaaaaattg catttaaacc taaaccggca ccagcaatat
     1261 taccaatatt aaaagctaga ccaccaaatg caatcaatat tgagataaca gtaccaagcc
     1321 caggaacaac tttatttgat atttcttgac ctcttaaacc agttacaact aatatgcgcc
     1381 atatattaat ttgtgcacca atgtcaatga tgatagacag taatatggca aatgcgaaac
     1441 ttgcaaaaaa ttgtgatgta aatactgctg tttgcgttaa aaatgctggc ccaattgcag
     1501 aagttgccat caaaaataca gaacctaata ataacctttt atgatttttt gtgaattcaa
     1561 agtcactttc ttttttaagc tttaaatttc ttcccatttt ttagccccct ataaggattg
     1621 aatatcaatg ccttctttca ttaaaatttc tctaattttc gaaacaaata ataatgcatg
     1681 ttctccatca ccatgcacac aaattgtatc tgcttgtaac gttacttcct tattgttttt
     1741 tgaaataact ttattttcct tcaccatctt taaaacctgc ttaagtgctt cgtcagtatc
     1801 agtaatcaca gcatcacttt cttttctact aacgagctgc ccatcatctt cgtatcgtct
     1861 atcagcaaac acttcagaag ctgtaattaa tccgacattc tttgcttctg aaattagata
     1921 tgaatttgct aatcctacta acactagtga tggatcaaag tcataaacag cttgtgctat
     1981 aacgcttgct atttctctgt cttttgcacc catctgatac aacgcaccat gcggtttaac
     2041 atgattaatt ttaacttgat gaatgcgaca aaacccttgt aatgcaccta attgataaat
     2101 catcaaatta taaatctcgt cgttagagat atctatattt cgtctgccaa agcctttcaa
     2161 atcaggtaaa ccaggatgtg cacctactgc aacattatgt gctttggcaa gttttaccgt
     2221 ttcattcatt acattttcat caccagcgtg aaaaccacaa gcaacattcg cacttgtaat
     2281 taacggaata atttgatgat caccaccaaa ggaataattt ccaaatgctt cgcctaaatc
     2341 acaattcaaa tcaactcgca ttataattcc acccctttaa caatttgatg tttttctaaa
     2401 aatttaatat caacatcttt tgcatctcca tcacgatata gtggataatt taaaactgca
     2461 tataaaaaat cggcagttgt agaaaatcca tctatcacca tttcatctaa ggtggctttc
     2521 aacttatcaa ttgctgaagc tctatcatga gattttacaa ttactttagc tactaaagaa
     2581 tcataatatg gtgaaacttg ataaccgtga tatagtaaag aatcgactcg cacattaaag
     2641 ccttgaggta aatgtaacgc tgtcacttta cctggtgttg gttgaaattt cttttcagga
     2701 ttttcggcat ttattctcgc ttctatcaca tgaccattaa attgaatatc gctttgtgaa
     2761 aaaggtaaat gattatgttc caataaatac aattgtgctg caaccaaatc acgttctgct
     2821 cgcatctctg taacagtatg ttcaacttgt attcgagcat tcatttcaat aaagtaatgt
     2881 gcggtatcag ttactaaaaa ttcaatcgta cctgcacttc tataatttgc tgcacgtgca
     2941 actttaacag catcgttaca tatttgttgt cgtctttctt cagttaatgc tgcacaagga
     3001 gattcttcga ttaatttttg atttttacgt tgtacagaac aatcacgttc ccctaaatgt
     3061 acaaaattat cctgcccatc tcccataact tgaacttcaa catgttttgc aacaggtata
     3121 aaagcctcaa cataaacacg atcatcatca aagtattttt ttccttcact tttagcttct
     3181 ttaaatgcct tttctaaatc ttcagctttc tttacaatac gtataccttt accaccaccg
     3241 ccactggcag ctttgataac aactggataa ccgatgtctt tggcaagctt ctcaatttca
     3301 gacacatggt tcacagcacc atttgatcct ggaatcacag gaacacctgc atgatgaact
     3361 gtttgtcttg ctgttatttt atcccccatc atttccatcg tttttttagt aggccctata
     3421 aacgctatgc cttgttcctc aacggtttga gcaaattttg ttgattctga taaaaagcca
     3481 tatcctgggt gaattgcatt agcaccagtg atttgtgcag cagatatgat gcggtcaata
     3541 tttaaataac tacctaaagc attagcttcc ccaatacata tagcttgatc tgctaaatgt
     3601 acatgcaagc tttgctcgtc cccttttgca taaactgcta cagtttcaat cccatattct
     3661 ctgcaagctc ttataatcct tacagcaatt tcacctctat tcgcaattaa acaacgaagc
     3721 atttacttac cccctttact taatacgtac caaaacttgg tcgtattcaa catttgttcc
     3781 atgatcagct actatttcag taatttctcc agcaacatct gttgttacct cgtttaatac
     3841 tttcatcgct tcaacatatc ctataatatc tcccttgtta actttgtcac cgacattcac
     3901 aattggttca gttaattctt tactatcttg taaaaagaat gtacctacca ttggtgattt
     3961 aatgtcatga taatcatttg tcgaaacatc ggagttatca ttcgcttttg aagctgtcaa
     4021 atcattattg ttcatacttt gatttgattg attactgtgt gcagccaaat gattcgagtc
     4081 agtgaagtca atttctattt catcttcaaa atttttatat ttaaatttct taacatcatt
     4141 ttccttcact aatttgatta tttgttcgat tttttcaata ttcattttac aaatcccctt
     4201 ttaaaattgt tgctaatttt ttcgaagtat gtcgcaagct agatgtatca aaaattggag
     4261 tcttttgatg actcttaaga atttcattaa acagagacat ttgttcccga ttcttatcta
     4321 cagcttcttg gaatgatatc catttaaatt gaattgtatc ttgtggtttc atctgtgcta
     4381 actttggcag atcaaatttg catacagttg caattttggt ataaccacct atcgtttgtt
     4441 tatcattaag cagaataata ggttgaccat catttggtac ttgaacacta ccaagagcaa
     4501 ccggttcaga aatgatatct gcttgattaa ttggtgcaac gctgtcacct tccaaacgat
     4561 agcccatacg gtctgattgt tcagtaatta aatatggatg atttacaatt ttcgctctag
     4621 cctcttcaga aaatgcctcg aattgaggtc cttgaagaat gtgtataata ttattttctg
     4681 gcaataaatc gtcctgtaaa tgaatcgtct ttccaatgtt ttctttaaag tcattattta
     4741 ttttcactgt tattacatca ttagctaata actttctacc tttgaatcct cctatactgc
     4801 ttcgggtatg tgttgcataa ctttcagcaa taggaggtac gttgatagaa tgaccaaaag
     4861 taagataacc gcgtgcacct ttggttatag cacctatttt taaaatgtca cctttctcag
     4921 ctaatatgac agaattcatt gatatagttt tattatttag cgaggcatta acactaccac
     4981 cggttataac aaatgtattt tgcgtattaa attgaatggt aggaccaatc aaagtatatt
     5041 caatcgctgg accatcattg ttaattaata actgcgcaac cttaaaacta aattgatcca
     5101 tggcacctgc gcctgaaaat ccaatatgtt cataaccttt tcttcctaga tcttgtaccg
     5161 ttgaaaagag acctggttgt aaaatcttaa ttgacatttt caatcaccac ccagtcatca
     5221 acattaaagt tgccatctga tatatctctt tcgatttgta taaattcttg ttcatctatt
     5281 gcataaaatt gtatccattc tcctgcttcg tacattgaca ttggttcacg ctcgctgcta
     5341 aatactttta acggtgtgcg tccaataatt tgccatccgc caggagaatc tgatggatat
     5401 agtcctgttt gattattcgc aatacctaca gaacctgcat gaatttttaa ccttggctga
     5461 ttacgtctag gtgtatgtag ttgttcatca agtccgccta agtatggaaa tcctggcata
     5521 aatcctagca tatatattaa ataaggttta cttgtatgtt tttcaataac ttgctcaaca
     5581 gttattcgat tatgctttgc tacttcttca atatctggtc catatgtacc accatattga
     5641 acaggtattt taataatacg attggtttga ttcacagcat gaacattttt ttcattaaat
     5701 ttgttaagtt ctaaattttc aattaattta gaagatgtta tagcttgttc atcaaaatat
     5761 attagaactg ctcgatacga agggacaata tcttgaattt ctaatatttc tttttctcgt
     5821 atccaccgta ccattgctgt gacattacga tatgtctctt cggatatttt attttcaaaa
     5881 taaatcataa ttgtctgctc gttaataaat cttacatcca ctttaaatcc ccctttgtat
     5941 tgcaataaac cagtattgaa taccttttca ttgtatcatt gagaagcaca agttgtttaa
     6001 taagtaattc aaatcgcata caataacaaa tgataatcaa tttgttttct cggaaatatt
     6061 ttgctttttt aaaatggagt agttaatatt gtaactctta cactaattta ggttctgcta
     6121 tcattcggtc tgatggaaaa tttttacttt tcatctgtcc gattttttga ttttgaatat
     6181 aaaaaagcac gaccgaagta tcattaacac acttcaatcg cgcaattaaa caatctattt
     6241 gatcatttat tggatattaa caatttttac gttcatttcg ccaccattag gtagtggaac
     6301 acgaacttca tcatctaaac ctttaccaat taacgcttta gccattggtg attcatttga
     6361 aatcttacca ttaaatgcat ctgattcagc tgaaccaacg atttgataac tttcctcttc
     6421 atcacctggt aattctacaa acgttactgt tttaccaatt ttaacaacgt tgttatctcc
     6481 agtatcttca atgattaatg catttcttaa catatgctca attctttgaa tatcttgttc
     6541 gatgaatcct tgttcatctt ttgctgcatc atactcagag ttctctgata agtcaccaaa
     6601 tgaacgtgca actttaattt tctctacaac ttcaggacgc ttaactgttt ttaattcttc
     6661 aagttcacgc tctaattttt caaaaccttc ttgagtcatt ggatattgct tttgattttc
     6721 catattgtca tcttccttta ctgaattata ctattgcttg ctaactaaag actgaatttt
     6781 tgttgtcata atatctattg caactttatt gctcccacct tcaggaataa ttatatcagc
     6841 atatttctta gtcggttcaa taaattggtc atgcataggt ctaacaacac ttaaatattg
     6901 attgataaca gagtccattg aacgcccacg ctctttagta tctcgtgtta aacggcgtaa
     6961 tattctcaag tctgcatctg tatcaacata tattttaaca tccatcatat cacgtaatac
     7021 cttattttct aaagcgaaaa taccttctac gataataaca tctttaggtt taaaatcaat
     7081 ggtaatgtca cttcttgtat gactagcata atcatatgtc ggtacttcta ctgctttacc
     7141 atttttcaag tctttaagat tttcaattaa taaatcatta tcgaatgcaa atggatggtc
     7201 ataattggtt tctaggcgct cgtcgaaagt caagtgcttt tgatctttat aatagtaatc
     7261 ttgagcaagt aaagcgacac tatgaccttc taagtttttc ataatttcgt tagttacagt
     7321 tgtttttcct gagccagatc caccagctat gccaatgatt gtagtagctt tcattagcca
     7381 atttcctttc tcatcatgtt gtttggatat atcgggcgat ccactttaat ttgaacgatt
     7441 tgtaatggat ggcgcgccgc gtctaagctg ttaccttctt catcataaat tgcttctact
     7501 acttgtgtaa atgtttcaat ttctggacca aagaattcta tttcttgacc tggtttaaag
     7561 ttatttcgtt gttgaatagt cgcaattttt gtatcttcat tatagtctaa taccaaacca
     7621 caaaaatcaa atggtgattt tttagattgt tgttgaccaa acatctgttc ttcataacca
     7681 ggtgttccct caaagaatgc tggtgcagtg tctctatttg cacatttatc taactctatt
     7741 aaccattccg gattaatctt aaaattgtca ggatctgccg catacgcatc aatgacttta
     7801 cgatatactg agacaactgt tgcaatataa tgaattgact tcatacgtcc ttcaattttt
     7861 aatgagtcca caccaatatc catcatttga ggaattgatt cgattaattt taaatcttta
     7921 ggactcatcg caaacggtgt aacttcacct tgattataaa aaacatcaag ttcaccatta
     7981 tcatcaactt ctaataattc ataatcccaa cggcaacttt gacagcaacc gcctctgttg
     8041 gaatcccttg cagtcatatg attacttaat gtacatctac ctgaataggc gatacacata
     8101 gcaccatgaa taaatgcttc gatttcaata tctacttttt ccttcatttc acgcatttcc
     8161 atcgcgccgg tctcacgtgc taatacaaca cgatccaatc cttcttcttt ccaatattct
     8221 acagctttgt aattagaaag tgattgttga gtagataaat gaatttcaag ttttggcgca
     8281 acttctttac atgtttcgat aattaaagga tctgcaacaa tgatacctgt cgcaccagtc
     8341 ttttccaaat tacgcaaata tgattctaga ccttcaatat tctcatcatg tgcaataata
     8401 tttgtcgtaa cataaatttt ggcaccgtaa cggttcgcaa attcaacacc ttcagctatt
     8461 tcttccatcg tgaaattatc agcatttgaa cgtaatccat attcttgacc acctaaaaat
     8521 acggcatcag cgccataatg tactgctatt tttaactttt ctaagtttcc agcaggtgct
     8581 aataattctg gtttcttcat aactgtttta ggagttgatt taatctcttc tattgtcttc
     8641 atgattaacc tccttaatat accgtttgtt tatataagaa accttcgtca aatggtcgat
     8701 gatcaggttg aatttcttca attgggtcca tcaacataaa tttctcatct tcatagattt
     8761 caggatcttc attgtacaaa tctatcgctt gacgatactg ttccgttacc acattaatat
     8821 attcttccgt ttgtagaata ccatcgattt taaatgaatc tatacccgcc tcaaaaaatg
     8881 gtgctaattc ttcaattaaa caaatgtcgt ttggtgacat aatgtgcgta ccattgtaat
     8941 cttcgtaaac tgggtaatta ttttgtcttt cttcatcata aagtaataaa gattgttcat
     9001 cattgcgacg ttcaattttc atttggcgat cttggaacgt ataataattg cctagtagca
     9061 tacgctttga ttggaacata caagtcattc cttgaacttg cacctcaatt tccacatttg
     9121 aattttcttt tatattaata atttcatcca aattcagctc acgtgctaag acagctcttg
     9181 atgcgcctct tttaccccag taattacatt gaaaatgatt agttactaac gtctctgcat
     9241 tccaatgaag tggtattgga ttctcttgcg ccttcacata cattactact gctggatccc
     9301 cgaaaataat tctgtcaact cgtatttcat gtaaaaaatt aatataatct tctacagcat
     9361 ctaaatgata attatgaaat aatccattca ccgccgcata tactttttta tcgtttttgt
     9421 gagctaatgc gacagcctct gtcatttgtt gtctattgaa ttcccctgga agtcttaaac
     9481 caaacttttg ctcgccaatt acaaaagcat ctgcacctaa atcaataagt gtttccatat
     9541 ggcttaatga cttgggtgtg acaagtaatt ctgtcatagt cattctcctt taattgaaat
     9601 cgctaatcca tcgtctatat ttaaaaaatt cgttgtatat cctggttgct ttattaacca
     9661 ctcattataa tcttgaacct ttttaaccat ttgtcttaca tttctcgatc taacaatccc
     9721 aatatccgat acaaaaccgt gatataaaac attatctgta attacgagac cttggtgctt
     9781 taaaagtggt gtatatattt caaaaaattt ctttgattgc gcttttgctg catcaataaa
     9841 tatcatatca taaactttgt catttacatt ttcaaattgc tctaaagcat taccttcaat
     9901 aattcgaacc tggttttcaa aatgataagt agctaaattt tgtttagcat attgaatcat
     9961 cgtttcatta cgctctatcg ttgtgacatg aatgtcatca gatatagaag cgaattgcat
    10021 agaactatag ccgattgctg taccaatttc taaaatattt ttaacattat tcatacgaat
    10081 taattgctta attaaatcta atgttaaacg atctacaatt ggcacttcat ttacttcggc
    10141 aaattcacgc aaaacttcga ttgaactatt ttgatgttga tgtaaatcta ttaaatattt
    10201 tttatttagg tcatccatgt tttaaacttc ctttatgtaa aataagtcaa tatgattatg
    10261 acaataaaat aaatcagcct tcacaattga ttataatttt gccaaccaat taaatgactg
    10321 atttcgtgtt agacgcaaag ctattttatt tatagaagcg aatcattcat ataaaattta
    10381 actttagata ttttaccata ttttcaataa aattataagc gttaattatt tatacattgc
    10441 ttaacttaaa aaaatactct tgcctcccca tcttaaggtt agcaagagta aaatcttttt
    10501 aattattctt ccatttcagt atttacaact tcttcaatca tgtcccattc ttcatcagtt
    10561 tcgattggta ctaacttacc accgtcacct gactcatctg gttcattgat cattggtaca
    10621 agctcaatca tatcgtcttc atctgattga gcaccttctt cagctaagat aacatactct
    10681 tttttgaatt caggatgata aaattctaaa acttttcggt ataaaacttc atttccctct
    10741 tcatcgaata aagttaataa ttcttcttcg ttattaattt ctagttgtga atcatgatta
    10801 tgttcagtca tagtaaaatc tccttttaat gtagtgaatc taaatagcct tgtaaaataa
    10861 ataccgctgc cattttatca atcacttgtt ttcttttttg tcttgaaaca tctgcttcta
    10921 ataatgatcg ttcagcagcc attgtgctta atctttcatc ccacatcaca atctcaatag
    10981 aaggataagc ttctaataat ttttctttat atgttaacga agcttcgcct cgaaatccta
    11041 ttgaattatt catgttttta ggtagtccta ttacgactgt acccacatta tgttttttaa
    11101 taatgtctac taattggtca atacctaatt cattattttc ttcattgatt cggagtgtgt
    11161 ctaatccttg tgccgtccaa cccattatat cactaattgc aattcctacc gttctactac
    11221 cgacatcgag tcctaaaatt ttatgttgta acataaatta tttattttgc tcttttaaat
    11281 agtaagaaac aagttcttcc ataataacat ctctatcaat atgacgaatt tgatttcttg
    11341 cttcattttg gcgtggaata tacgcagggt cacctgataa taaataacct acaatttggt
    11401 ttacggcatt atatcctcgt tcatctaatg ttcgataaac attatttaaa acatctctta
    11461 catcttgcgt tggaagttct tcatagtcga atttcattgt tttatcaaag ttttccattt
    11521 gcgacactcc tttaattaca aatataactc actatcatca tacaatatta tggctttaaa
    11581 ttatagattt ttaatgtaat ctttaataaa gcttaatgat tttgagatat tttcaggttg
    11641 tgtaccgcca ccttgagcca tatctggacg accgccacct ttaccaccaa cgattggtgc
    11701 catttgtttg ataagatcac cggctttaac gttatttgtt aaagatttag ggacagttgc
    11761 aaccatcgat actttatcat caacattact tgcaagaatg ataattgtat cttgtagttt
    11821 agatttaaaa tcgtccattg tcgagcgaat tgctttcgca tttggtacat ccacttcagt
    11881 aaccaatact ttatagccat tgatttcttc aacttgattt tcaatattac ccattttaag
    11941 tgatgtgatt tctttgtcac gttgctctaa ttgttttaat aatgcttttt cttcatcttg
    12001 taattgtgtt aacttatcga ctacttgatc atcagatttc actttcagct gtgatttcat
    12061 cgtattaaat ttctcttgaa tatcttctaa atataagaaa gctgctttac ctgttaatgc
    12121 ttcaatacga cgcacaccag ctcctgtacc tgactcactt actattttga ataagccaat
    12181 ttcagaagta ttgcggacat gaataccacc acataattca attgaaaatg gtgccatatt
    12241 tactacacgc acaacatcac catatttttc accgaataat gccattgcgc ccatttcttt
    12301 agctgaagca atatccattt cttgaatgtt aacgtcaata cctttccaaa tttcttcatt
    12361 tactaagcgt tcaacttgat caatttcatc attagtcatt ggaccaaaat gagagaaatc
    12421 aaaacgtaaa cgatctgctt ctactagtga accagcttgg ttaacatgat cacccagtac
    12481 tgatttcaac gctgcatgta ataaatgtgt tgcactatgg ttcttttgaa tgtcacgtcg
    12541 atcattttgg ttcacttcag cagacactgt agcgccaaca tttacttggc caaattgtac
    12601 tactccttta tgcaagtttt gaccatttgg tgctttggtt acttcactaa cagcaatttc
    12661 aaaattgtca ttataaacaa tacctgtatc cgcaacttgt ccaccactga ctgcataaaa
    12721 tggtgtttcc gttaacatga agtatactgt ttcacccgct tcaacttgtg aaacttcttc
    12781 accattatat atcaagtgtg ttagtgttgt ttgagctgtc gcagtatcat aaccaacaaa
    12841 agtacttgca gatgtaatat ttttcaatac ttcactttga acttgcattg attgagaatt
    12901 ttgacgtgct tgacgtgcac gatcacgttg ttgttgcatt tctgactcga atgttgtcat
    12961 atcaactttc aatcctgctt gcactgctat ttcttcagtt aattcaattg ggaacccgta
    13021 cgtatcatac aatttaaatg catctttccc attaatttca tttgttgtcg ctttagcttt
    13081 tttaattaat tcatttaaaa tcgctaaacc atcttctaat gtttcatgga atcgttcttc
    13141 ttcagacttt ataacacgct taatgaaatc tgctttttcc ttaacatttg gataatatgg
    13201 ttccataatg tctgcaacaa tatcaacaag tttgtacata aatggctcat tgattcctaa
    13261 cgtttgacta aaacgaacgg cacgacgtaa caatcgacgt aatacatatc ctctaccttc
    13321 attggcaggt aatgcaccat cagaaattgc aaatgcaatc gtacgaatgt ggtcagcaat
    13381 tactttaaat gccacatctt gttcgttgtt tactaaatat tgtttacctg atactttttc
    13441 gatttcattc attataggca taaataaatc tgtttcatag ttagtacgta cattttgaga
    13501 aactgaggcc atacgctcaa gccccatgcc agtatcaatg tttttattag gtaatggtgt
    13561 gtaactatga tctttattat gattgaattc actaaatact aagttccata cttcaagata
    13621 gcgttcattt tctccacctg gatacatttc ttctgccgga tcgtcttgtc catatgcttc
    13681 tccgcgatca tagaaaatct cagtgtttgg tcctgaaggc ccttcaccaa tatcccagaa
    13741 gttaccttca atgcgaataa tacgactttc ttcaagccca atatctttat gccaaatgtt
    13801 gtatgcttcc atatcttccg gatgaatcgt aacgtacaat ttatctggct ccatccccat
    13861 ccatttatca ctcgttaaaa attcccaagc aaattcaatc gcttcttgtt taaaataatc
    13921 accaattgag aagttaccta acatttcaaa gaatgtatgg tgacgcgctg tgaaaccaac
    13981 attttcaata tcatttgtac gaatagcttt ttgagagttt acaattcttg gctttttagg
    14041 tgtttcacgt ccatcaaaat atttctttaa tgttgctaca cctgaattaa tccataataa
    14101 tgtatcatca tcaattggca ctaatggtgc agaaggttca accatatgtc ctttttcaac
    14161 aaagaaatct agatattttt gtctaatttc actcgctttt aactttttca tcttttacac
    14221 atcctattta ctgtttttaa attaccattc cataaaaatt gatgacacag atagtcgatt
    14281 tgaaaaacta gtataaatca atatcatttt ttattattaa aaaataaaaa acgcccatcc
    14341 tcaaaaggga cgaacgttat cgcggtacca ccctagttat aaatgcaatt caacacattt
    14401 atcactttaa atcgactata cagttgtgca taaagtagcg ttcactaatg tttgttgtac
    14461 ttttcaccaa ccagtacatc tctgataaac aaatcattaa ctactcatct ttatacgaat
    14521 ttaattctat tttagttaca tttactcttg ttgtcaacgt tctataaagt catacggcgt
    14581 gatttctccc atattaatca ttgggtcaat tttaaacatt gtagcttccg ttaatacatt
    14641 tatatctgtt tttgttgaat cagacgtaac ttctccacta tcattcgatg acattggcgc
    14701 ttctacttga tcatctattg tcgtttgtga agctcctgta tcattagttg ctgtgttttc
    14761 cagcatttct tcatcttctg aattaaaata atttttcaac aatgtacata attgtgttaa
    14821 acgcgcttga ccatttgttt ttaatccaat atcaaatgct tctggatcac caagtagaac
    14881 taaactcgtt ttcgctctag ttaaaccagt atataatatc ggtctttgta acattctaaa
    14941 atactgttta acaataggca tgataacaat aggaaattct gaaccttgtg atttatggat
    15001 tgatgtacaa taagcatgtg ttaattccat catatcttgt ttcgtaaatg taatttcatt
    15061 accttcaaaa tccacaacaa gtacatcttt attaagggca ttttctttcg cccaaaaaat
    15121 accaacaata actcctatgt caccattgaa tatgttatca tttggcctat taacaagttg
    15181 taatactttg tcaccttttc taaagactac atcaccaaac tcaatttctc gtgtgtcttt
    15241 ctttttaggg tttaaaatat cttgtaaaac ttgatttaaa cgtttaatac cggcatttcc
    15301 tttatacatt ggtgcaagca cttgaatatc agccatagta taccctttat taacagcact
    15361 agtaactacc ttctctacaa ctgttggtat ttggtttgcc tgacagttaa taaaacttct
    15421 atcatgaaaa cgctgtgtaa tatcaatttt ctgacccaac ttcattcgat gtgctaattc
    15481 tataatgctt gaaccatctt gttgacgata tacttcagtc agattaactc gtggtatagc
    15541 tttcgattca attaaatctt taaatacttg accaggacct acagaaggca attggtcctc
    15601 atcacctaca aatatcaatt gtgcatctaa aggaactgca cttaaaaatt ggtggaacaa
    15661 ccaagtatct accatagaca tctcatcaat gattatgagt cgtgcgttta tttcattttc
    15721 taatatatcc tctggctttg tgtcttgatt ccaacctatt aaacgatgaa tcgtcatagc
    15781 ttctaatcca gttgactctt gtagtctctt ggacgctctt cctgttggcg ctgctaatac
    15841 aactggataa tcatcattga cataatcatc ataatctaat gataagccat gaatctcagc
    15901 atataattca acaatacctt taattactgt cgtttttcct gttcccggtc caccggttaa
    15961 tagcatcacc ttagaattga tagccgtttg caaagcttct ttttgtgaag ctgcatagtt
    16021 cacttgattc gcatcttcta tttcaccaat atgcatttgt aaatctgact gttcaatttc
    16081 tgtaagttta tttgtatgcg tctttattct gaataagttt tgaacacttt tgatttcaga
    16141 ataatacaaa cttggaattg caacttgttc attgtcaata attagtcgtt tttcctcatt
    16201 taagtattgc aacatttcgt ctaatttttc aggttcgatg acctcttcat cttgataatt
    16261 taatacatca accgttaaat ctataacaac attgataggc aaatatgtat gtccctgttt
    16321 aatacattct tcttctaacg tatagagcaa cgcagctttt aatcgttcat tatcgttata
    16381 agcgatacca atatttctag caagttgatc tgctttatta aaaccaatac ctttaatatc
    16441 ataaatcaat tgatatggat ttcgatctaa aatagtcagt gtatcgccga gataaaactg
    16501 ataaattgcc attgaaagtt taggaccaaa ccctaaatca tgtaaacgaa tcattatttt
    16561 ttcagattct tgatttgctg aaatttgttc tgcaatttgt ttctgtttct ttttagataa
    16621 tcccgaaact ttttctagca ctgaatggtc atctaatata tcatttatcg cattgtcacc
    16681 taatgtatta acaatatttt gagctgtctt tttacctaca cctttaaaca aatcactaga
    16741 taaataactt ataattgctt ctttcgtttg tggcatttct ttttcaaaag tctctgcttt
    16801 taattgttta ccataacgtg gatgatcaac aacttgccct ttaaatgtgt agacatcgcc
    16861 ttcaacaata ttcggaagaa accctacaac agttggcatt gtatcaaagt cttcatttgt
    16921 ttcaatagta tctactttaa gcactgtata aaaattatca ctgttttgaa acaatatcgc
    16981 ttcaacagta cctttgatca ttgaataatc aaatagtgta gggtctgaca tgttactcct
    17041 cctctttcat tttagtgaat gttttcagcg catgctgact taataagtgt ttagggtcga
    17101 tagtcacagc ttctttaaaa tgagttattg cttcatcaat atcttcattt ttcataaata
    17161 acgctaagcc caaattgtat cttgcatcaa catgattttt atcaatcgtt aatacatgtt
    17221 taagttgagt tatggcttca ttaaacattt ctaattgaca taatacaaga ccatattgaa
    17281 attgaacttc tgcatctttg tctttatcta gttccgcagc agtcattaaa tacggcaatg
    17341 ccagcttaaa tgattctaac tgattaaacg ccataccgat catataatta caatcaactt
    17401 gttcaatctc tgtttgtaat gcttgttgat ataatttaat agcttcttga taacgttgct
    17461 gattataata tacatttgct agattataaa atacgacgcc attcttcgga tctattgtta
    17521 aagctttttg gaaaaagcgc tctgcctttt caatctcatt cgcatcagca agtacgatac
    17581 cagcattaat ataattttca ataattgtag gattttcttc gatatttccg aacaatgctt
    17641 gtaacgcttc ttctattttt ccattttgta tgtattgata aattgtttgt tgatctatca
    17701 tttacgaacc tcatttctca tcaattataa catcttgata aattgtatgt ctcgaatcac
    17761 ttaacaacga ataaaatata atctaatatc atcttcattc atgaaaaagc gggaatggaa
    17821 tagaaatgat taagaaccat taacggttta ttatgtaatg gttcttccac attagccact
    17881 actattatgt acttaaaaat aggaatacat aattagactc atgcataggg agtgggacag
    17941 aaatgatatt ttaacaaaat taaattcgtt tatccccaac tcgcattgcc tgtagaattt
    18001 ctttacgaaa ttctctgtgt tggggcccca tccccaactt gcaaattatt gaaatctgac
    18061 ttttggtcag cttctgtgtt ggggccccgt ccccaacttc cattgcctgt tgaaattggg
    18121 gtcccaattt ctctgtgttg gggccccgcc aatataacat tgtagagcct aggacattgt
    18181 gatgtcccag actctatcat catgaattat tctcatcaaa aactgtcttt cgtcattttc
    18241 gacgttgaaa cttcaaataa gtaatttatt gttgccattg tttatacaac ataatttaat
    18301 tgaccttcat ttttgaacac atcgtcaatt gttgctccac caagacacac atcaccttga
    18361 taaaaaacaa ctgcttgtcc aggtgtgatt gctcttactg gctcagcaaa agtaacacgt
    18421 agtgcatggt cgttttcacg tttcacaaaa actttcgtat ctttttggcg atatctaaat
    18481 ttagctgtac attcaaaacc ttgatctaag tcattatctt ctggatttac aaatgaatag
    18541 tctgaagcaa ttaagtaatc actgtataat gcatcgtgat ggaatccttg ttctacatat
    18601 aaaacattat cttttagatt tttaccgaca acaaaccaag gatcgccatc tccacctata
    18661 cctaaaccat gtctttgtcc tattgtgtaa tacatcaacc cactatgttt acccattttc
    18721 ttaccatcaa gtgttatcat atcacccggt tgcgcaggta gatattgaga taaaaatgtt
    18781 ttaaagtttt tttcgccgat aaaacaaata cctgtagaat cttttttctt agcagtaact
    18841 agtccttgtt cttcagcaat tcgacgcact tcaatctttt cgatgtcgcc aattgggaac
    18901 atcacttttg aaagttgttg ttgggataat tgattcaaga agtatgtttg atctttatta
    18961 ttatctacac cacgtaacat ttcaacatga ccatcttcat gacgatgtat gcgtgcgtaa
    19021 tgccctgttg ctacataatc tgcacctaaa ttcatcgcat gatctaaaaa ggctttaaac
    19081 ttaatttctt tattacacat aacgtctggg tttggagtac gacctttttt atattcatct
    19141 aagaaatacg taaagacttt atcccaatat tctttttcaa aattaacagc gtaatatgga
    19201 atgccaattt gattacacac ttcaataaca tcgttgtaat cttcagttgc agtacatacg
    19261 ccattttcgt cagtgtcatc ccagtttttc ataaatatgc caatgacatc ataaccttgt
    19321 tcttttaaga cgtgggctgt tacagaacta tctacaccgc ctgacatacc aacgacaaca
    19381 cgtatatctt tatttgacaa ttatgactcc tccttaaatt taaaatatat tttatgaatt
    19441 tcagctacaa ttgcattaat ttcattttca gtagtcaatt cgttaaaact aaatcgaatc
    19501 gaatgatttg atcgctcttc atcttcgaac attgcatcta aaacatgcga cggttgtgta
    19561 gagcctgctg tacatgcaga tccagacgac acatagattt gtgccatatc caacaatgtt
    19621 aacatcgttt caacttcaac aaacggaaaa tatagattta caatatggcc tgtagcatcc
    19681 gtcattgaac catttaattc aaatggaatc gctctttctt gtaatttaac taaaaattgt
    19741 tcttttaaat tcattaaatg aatattgtta tcgtctcgat tcttttctgc taattgtaat
    19801 gctttagcca tcccgacaat ttgtgcaaga ttttcagtac ctgcacggcg tttcaattct
    19861 tgttcaccgc caagttgagg ataatctaat gtgacatggt ctttaactaa taatgcaccg
    19921 acaccttttg gtcctccaaa cttatgagca gtaatactca ttgcgtcgat ctcaaattcg
    19981 tcaaacttaa catcaagatg tccaattgct tgaaccgcat caacatggaa atatgcattt
    20041 gtctcagcaa taatatcttg aatatcataa atttgttgca ctgtgccaac ttcattattt
    20101 acaaacataa tagatactaa aatcgtctta tctgtaattg tttcttcaag ttggtctaaa
    20161 tcaatagcac ctgtatcatc aacatctaga tatgttacat caaaaccttc tcgctctaat
    20221 tgttcaaaaa catgtaacac agaatgatgt tcaatcttcg atgtgataat gtgattaccc
    20281 aattgttcat ttgcttttac tatgccttta attgccgtat tattcgattc tgttgcacca
    20341 ctcgtaaata taatttcatg tgtatctgca ccaagtaatt gtgcaatttg acgtcttgac
    20401 tcatctaaat atttacgcgc atctcttccc ttagcatgta ttgatgatgg attaccataa
    20461 tgcgaattgt aaatcatcat catcgcatct actacttcag gttttactgg tgtggtcgca
    20521 gcataatctg cataaatttc catgtttgga cactcctcac aattttatca atgttccaat
    20581 aatagcacct tacatactat ttttctactt ttctgtttaa ctttatttat aatgttttta
    20641 attatatttt accattttct acacatgctt ttcgataggc ttttttaagt ttatcgcttt
    20701 attcttgtct tttttataaa ttttagtatt tgcagatatt ttttcattcg taaaatgtaa
    20761 cgtactatta ttttggttat gagcaattta atatttatct ggttattcga ttggtatact
    20821 tcttatatca tacaaaagga aggacgatac aaaaatggcg attaaatatt cagcattaaa
    20881 ccttgtccct attcgagaag gtgaagatga acgaacagca attaatgata tggttaagct
    20941 cgcacaacat ttagacgaat tatcatatga aagatattgg attgctgaac accataacgc
    21001 tcccaaccta gtaagttcag cgactgcttt attgattcaa catacgttag aacatacgaa
    21061 acacatacgt gtaggttctg gaggaatcat gttacctaac catgctccat taatcgttgc
    21121 ggaacaattt ggcacgatgg caacattatt tccaaatcgt gtcgatttag gattaggacg
    21181 tgcacctgga acagatatga tgaccgcaag tgcattaaga cgagatcaac atgatggtgt
    21241 ttataaattt ccggaagaag tttcattatt acaacaatat ttcggtcccg ctcaccaaca
    21301 agcatatgtt cgtgcttatc cagcagtagg taaaaatgtg cctttatata ttcttggttc
    21361 ttcaacagat tctgcacatt tagctgctcg taaagggctt ccatatgtgt tcgctggaca
    21421 ttttgcacct caacaaatga aagaagctat cgaaatttac aaaacgttat ttgaaccttc
    21481 tgatgtatta gacgaacctt atgttattgt atgtttaaat acaatcgttg ctgaaaatga
    21541 tgacgaagca caatatttag cttcatctat ggcacaagta atggttagta tcactcgtgg
    21601 cagaatgcag cccgttcaac cgccaacaca tgaactacaa aatatattaa cgccgagaga
    21661 atatgcgatg gctatggaaa gacagaaaat atcattaata ggttcagaaa atactgttca
    21721 acaaaaaatt caagatttta tggaaactta tggtgaagtc aacgaaatta tggcaataag
    21781 ttatatttat gataaagata tgcaattaga ctcttatcgt cggttcaaga atgttataaa
    21841 tcagataaat gaaaaaaaca ctttataatg tgataaataa ac
//
LOCUS       DAMZKC010000029        21461 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.029, whole genome shotgun sequence.
ACCESSION   DAMZKC010000029 DAMZKC010000000
VERSION     DAMZKC010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 21461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21461
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.029"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(92..1057)
                     /locus_tag="POB55_000830"
     CDS             complement(92..1057)
                     /locus_tag="POB55_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HDI6499805.1"
                     /translation="MKKIMITGALGQIGTELVVKCREIYGTDNVLATDIREPEADSPV
                     QNGPFEILDVTDRDRMFELVRDFEADSLMHMAALLSATAEKNPILAWDLNMGGLMNAL
                     EAARTYNLHFFTPSSIGAFGDSTPKVNTPQVTIQQPTTMYGVNKVAGELLCQYYFKRF
                     GVDTRSVRFPGLISHVKEPGGGTTDYAVEIYFKAVREGHYTSFIDKGTYMDMMYMDDA
                     IEAIIKLMEADDAKLETRNGYNLSAMSFDPEMVKEAIQEYYPNFTLDYDVDPIRQGIA
                     NSWPDSIDTSCSRGEWGFDPKYDLASMTKLMLEAIEQKDTVKNNN"
     gene            complement(1271..2908)
                     /locus_tag="POB55_000831"
     CDS             complement(1271..2908)
                     /locus_tag="POB55_000831"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HDI6499806.1"
                     /translation="MSYSIGIDYGTASGRVFLINTTNGQVVSKFVKPYTHGVIESELN
                     GLKIPHTYALQNSNDYLEIMEEGISYIVRESKIDPDNIVGIGIDFTSSTIIFTDENLN
                     PVHNLKQFKNNPHAYVKLWKHHGAYKEAEKLYQTAIENNNKWLGHYGYNVSSEWMIPK
                     IMEVMNRAPEIMEKTAYIMEAGDWIVNKLTNKNIRSNCGLGFKAFWEEETGFHYDLFD
                     KIDPKLSKVIQDKVSAPVVNIGEAVGKLDDKMAQKLGLSKETMVSPFIIDAHASLLGI
                     GSEKDKEMTMVMGTSTCHLMLNEKQHQVPGISGSVKGAIIPELFAYEAGQSAVGDLFE
                     YVAKQAPKSYVDEAANRNMTVFELMNEKIKHQMPGESGLIALDWHNGNRSVLSDSNLT
                     GCIFGLTLQTKHEDIYRAYLEATAFGTKMIMQQYQDWHMEVEKVFACGGIPKKNAVMM
                     DIYANVLNKKLIVMDSEYAPAIGAAILGAVSGGAHNSINDAVDAMKEPILYEINPEAE
                     KVQRYETLFKAYKALHDIHGYKKANIMKDIQSLRVEG"
     gene            complement(3067..3945)
                     /gene="hchA"
                     /locus_tag="POB55_000832"
     CDS             complement(3067..3945)
                     /gene="hchA"
                     /locus_tag="POB55_000832"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HDI6499807.1"
                     /translation="MSQDVNELSKQPTPDKAEDNAFFPSPYSLSQYTAPKTDFDGVEH
                     KGAYKDGKWKVLMIAAEERYVLLENGKMFSTGNHPVEMLLPLHHLMEAGFDVDVATLS
                     GYPVKLELWAMPTEDEAVISTYNKLKEKLKQPKKLADVIKNELGPDSDYLSVFIPGGH
                     AAVVGISESEDVQQTLDWALDNDRFIVTLCHGPAALLSAGLNREKSTLEGYSVCVFPD
                     SLDEGANIEIGYLPGRLKWLVADLLTKQGLKVVNDDMTGRTLKDRKLLTGDSPLASNE
                     LGKLAVNEMLNAIQNK"
     gene            complement(4212..5399)
                     /locus_tag="POB55_000833"
     CDS             complement(4212..5399)
                     /locus_tag="POB55_000833"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HDI6499808.1"
                     /translation="MVQSLHEFLEENINYLKENGLYNEIDTIEGANGPEIKINGKSYI
                     NLSSNNYLGLATNEDLKSAAKAAIDTHGVGAGAVRTINGTLDLHDELEETLAKFKGTE
                     AAIAYQSGFNCNMAAISAVMNKNDAILSDELNHASIIDGCRLSKAKIIRVNHSDMDDL
                     RAKAKEAVESGQYNKVMYITDGVFSMDGDVAKLPEIVEIAEEFGLLTYVDDAHGSGVM
                     GKGAGTVKHFGLQDKIDFQIGTLSKAIGVVGGYVAGTKELIDWLKAQSRPFLFSTSLA
                     PGDTKAITEAVKKLMDSTELHDKLWDNAQYLKNGLSKLGYDTGESETPITPVIIGDEK
                     TTQEFSKRLKDEGVYVKSIVFPTVPRGTGRVRNMPTAAHTKDMLDEAIAAYEKVGKEM
                     KLI"
     gene            5570..6745
                     /locus_tag="POB55_000834"
     CDS             5570..6745
                     /locus_tag="POB55_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDI6499809.1"
                     /translation="MLDWFQLANNKENKTIQLRRYLHQYPELSFEEFQTHDYIVNQLS
                     QLSCDIETPIGRNGIKATFKGLGTGPTIALRADFDALPVEELNDVPYKSKNPGCMHAC
                     GHDGHTAILLTVAEILDEHKHLLEGNVVLIFQYGEEIMPGGSQEMIDAGCLENVDRIY
                     GTHLWSGYPTGTIHSRAGAIMASPDEFSVTIKGRGGHGAKPHETIDPIVIMAEFILSA
                     QKIISRTIDPVKQAVLSFGMIQAGTTDSVIPDQAFCKGTVRTFDSDIQNHVMDKMDKL
                     LQGLAIANDINYDLNYIKGYLPVHNNEKAYQVIKEATNDLHVRFNESDLMMIGEDFSH
                     YLKVRPGAFFLTGCGNESKGITAPHHNPKFDIDEKSLKYAVAVFLKIIELEQVFKTN"
     gene            complement(7027..8211)
                     /gene="tuf"
                     /locus_tag="POB55_000835"
     CDS             complement(7027..8211)
                     /gene="tuf"
                     /locus_tag="POB55_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDI6499810.1"
                     /translation="MAKEKFDRSKEHANIGTIGHVDHGKTTLTAAIATVLAKNGDSVA
                     QSYDMIDNAPEEKERGITINTSHIEYQTDKRHYAHVDCPGHADYVKNMITGAAQMDGG
                     ILVVSAADGPMPQTREHILLSRNVGVPALVVFLNKVDMVDDEELLELVEMEVRDLLSE
                     YDFPGDDVPVIAGSALKALEGDAQYEEKILELMEAVDTYIPTPERDSDKPFMMPVEDV
                     FSITGRGTVATGRVERGQIKVGEEVEIIGLHDTSKTTVTGVEMFRKLLDYAEAGDNIG
                     ALLRGVAREDVQRGQVLAAPGSITPHTEFKAEVYVLSKDEGGRHTPFFSNYRPQFYFR
                     TTDVTGVVHLPEGTEMVMPGDNVEMTVELIAPIAIEDGTRFSIREGGRTVGSGVVTEI
                     IK"
     gene            complement(8428..10509)
                     /gene="fusA"
                     /locus_tag="POB55_000836"
     CDS             complement(8428..10509)
                     /gene="fusA"
                     /locus_tag="POB55_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDI6499811.1"
                     /translation="MAREFSLEKTRNIGIMAHIDAGKTTTTERILYYTGRIHKIGETH
                     EGASQMDWMEQEQDRGITITSAATTAAWEGHRVNIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFVNKMDKLGANFEYSVSTLHDRLQANAA
                     PIQLPIGAEDEFEAIIDLVEMKCFKYTNDLGTEIEEIEIPEDHLDRAEEARASLIEAV
                     AETSDELMEKYLGDEEISVSELKEAIRQATTNVEFYPVLCGTAFKNKGVQLMLDAVID
                     YLPSPLDVKPIIGHRASNPEEEVIAKADDSAEFAALAFKVMTDPYVGKLTFFRVYSGT
                     MTSGSYVKNSTKGKRERVGRLLQMHANSRQEIDTVYSGDIAAAVGLKDTGTGDTLCGE
                     KNDIILESMEFPEPVIHLSVEPKSKADQDKMTQALVKLQEEDPTFHAHTDEETGQVII
                     GGMGELHLDILVDRMKKEFNVECNVGAPMVSYRETFKSSAQVQGKFSRQSGGRGQYGD
                     VHIEFTPNETGAGFEFENAIVGGVVPREYIPSVEAGLKDAMENGVLAGYPLIDVKAKL
                     YDGSYHDVDSSEMAFKIAASLALKEAAKKCDPVILEPMMKVTIEMPEEYMGDIMGDVT
                     SRRGRVDGMEPRGNAQVVNAYVPLSEMFGYATSLRSNTQGRGTYTMYFDHYAEVPKSI
                     AEDIIKKNKGE"
     gene            complement(10632..11102)
                     /gene="rpsG"
                     /locus_tag="POB55_000837"
     CDS             complement(10632..11102)
                     /gene="rpsG"
                     /locus_tag="POB55_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HDI6499812.1"
                     /translation="MPRKGSVPKRDVLPDPIHNSKLVTKLINKIMLDGKRGTAQRILY
                     SAFDLVEQRSGRDALEVFEEAINNIMPVLEVKARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVNYARLRGEKTMEDRLANEILDAANNTGGAVKKREDTHKMAEANKAFAHYRW"
     gene            complement(11168..11581)
                     /gene="rpsL"
                     /locus_tag="POB55_000838"
     CDS             complement(11168..11581)
                     /gene="rpsL"
                     /locus_tag="POB55_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HDI6499813.1"
                     /translation="MPTINQLVRKPRQSKIKKSDSPALNKGFNSKKKKFTDLNSPQKR
                     GVCTRVGTMTPKKPNSALRKYARVRLSNNIEINAYIPGIGHNLQEHSVVLVRGGRVKD
                     LPGVRYHIVRGALDTSGVDGRRQGRSLYGTKKPKN"
     gene            complement(11679..11933)
                     /locus_tag="POB55_000839"
     CDS             complement(11679..11933)
                     /locus_tag="POB55_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HDI6499814.1"
                     /translation="MSKEKVARFNKQHFVVGLKETLKALKKDQVTSLIIAEDVEVYLM
                     TRVLSQINQKNIPVSFFKSKHALGKHVGINVNATIVALIK"
     gene            complement(12070..15666)
                     /gene="rpoC"
                     /locus_tag="POB55_000840"
     CDS             complement(12070..15666)
                     /gene="rpoC"
                     /locus_tag="POB55_000840"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HDI6499815.1"
                     /translation="MKIGLASPEKIRSWSFGEVKKPETINYRTLKPEKDGLFCERIFG
                     PTKDWECSCGKYKRVRYKGMVCDRCGVEVTKSKVRRERMGHIELAAPVSHIWYFKGIP
                     SRMGLLLDMSPRALEEVIYFASYVVVDPGPTGLEKKTLLSEAEFRDYYDKYPGQFVAK
                     MGAEGIKDLLEEIDLDEELKLLRDELESATGQRLTRAIKRLEVVESFRNSGNKPSWMI
                     LDVLPIIPPEIRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLDLGAPGIIVQNEK
                     RMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGKRVDYSGRSV
                     IAVGPSLKMYQCGLPKEMALELFKPFVMKELVQREIATNIKNAKSKIERMDDEVWDVL
                     EEVIREHPVLLNRAPTLHRLGIQAFEPTLVEGRAIRLHPLVTTAYNADFDGDQMAVHV
                     PLSKEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTLERKDAVNTGAIFN
                     NTNEVLKAYANGFVHLHTRIGVHASSFNNPTFTEEQNKKILATSVGKIIFNEIIPDSF
                     AYINEPTQENLERKTPNRYFIDPTTLGEGGLKEYFENEELIEPFNKKFLGNIIAEVFN
                     RFSITDTSMMLDRMKDLGFKFSSKAGITVGVADIVVLPDKQQILDEHEKLVDRITKQF
                     NRGLITEEERYNAVVEIWTDAKDQIQGELMQSLDKTNPIFMMSDSGARGNASNFTQLA
                     GMRGLMAAPSGKIIELPITSSFREGLTVLEYFISTHGARKGLADTALKTADSGYLTRR
                     LVDVAQDVIVREEDCGTDRGLLVSDIKEGTEMIEPFIERIEGRYSKETIRHPETDEII
                     IRPDELITPEIAKKITDAGIEQMYIRSAFTCNARHGVCEKCYGKNLATGEKVEVGEAV
                     GTIAAQSIGEPGTQLTMRTFHTGGVAGSDITQGLPRIQEIFEARNPKGQAVITEIEGV
                     VEDIKLAKDRQQEIVVKGANETRSYLASGTSRIIVEIGQPVQRGEVLTEGSIEPKNYL
                     SVAGLNATESYLLKEVQKVYRMQGVEIDDKHVEVMVRQMLRKVRIIEAGDTKLLPGSL
                     VDIHNFTDANREAFKHRKRPATAKPVLLGITKASLETESFLSAASFQETTRVLTDAAI
                     KGKRDDLLGLKENVIIGKLIPAGTGMRRYSDVKYEKTAKPVAEVESQTEVTE"
     gene            complement(15830..19381)
                     /gene="rpoB"
                     /locus_tag="POB55_000841"
     CDS             complement(15830..19381)
                     /gene="rpoB"
                     /locus_tag="POB55_000841"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDI6499816.1"
                     /translation="MAGQVVQYGRHRKRRNYARISEVLELPNLIEIQTKSYEWFLREG
                     LIEMFRDISPIEDFTGNLSLEFVDYRLGEPKYDLEESKNRDATYAAPLRVKVRLIIKE
                     TGEVKEQEVFMGDFPLMTDTGTFVINGAERVIVSQLVRSPSVYFNEKIDKNGRENYDA
                     TIIPNRGAWLEYETDAKDVVYVRIDRTRKLPLTVLLRALGFSSDQEIVDLLGDNEYLR
                     NTLEKDGTENTEQALLEIYERLRPGEPPTVENAKSLLYSRFFDPKRYDLASVGRYKTN
                     KKLHLKHRLFNQKLAEPIVNTETGEIVVEEGTVLDRRKIDEIMDVLESNANSEVFELH
                     GSVIDEPVEIQSIKVYVPNDDEGRTTTVIGNAFPDSEVKCITPADIIASMSYFFNLLS
                     GIGYTDDIDHLGNRRLRSVGELLQNQFRIGLSRMERVVRERMSIQDTESITPQQLINI
                     RPVIASIKEFFGSSQLSQFMDQANPLAELTHKRRLSALGPGGLTRERAQMEVRDVHYS
                     HYGRMCPIETPEGPNIGLINSLSSYARVNEFGFIETPYRKVDLDTHAITDQIDYLTAD
                     EEDSYVVAQANSKLDENGRFMDDEVVCRFRGNNTVMAKEKMDYMDVSPKQVVSAATAC
                     IPFLENDDSNRALMGANMQRQAVPLMNPEAPFVGTGMEHVAARDSGAAITAKHRGRVE
                     HVESNEILVRRLVEENGVEHEGELDRYPLAKFKRSNSGTCYNQRPIVAVGDVVEYNEI
                     LADGPSMELGEMALGRNVVVGFMTWDGYNYEDAVIMSERLVKDDVYTSIHIEEYESEA
                     RDTKLGPEEITRDIPNVSESALKNLDDRGIVYIGAEVKDGDILVGKVTPKGVTELTAE
                     ERLLHAIFGEKAREVRDTSLRVPHGAGGIVLDVKVFNREEGDDTLSPGVNQLVRVYIV
                     QKRKIHVGDKMCGRHGNKGVISKIVPEEDMPYLPDGRPIDIMLNPLGVPSRMNIGQVL
                     ELHLGMAAKNLGIHVASPVFDGANDDDVWSTIEEAGMARDGKTVLYDGRTGEPFDNRI
                     SVGVMYMLKLAHMVDDKLHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGA
                     AYTLQEILTYKSDDTVGRVKTYEAIVKGENISRPSVPESFRVLMKELQSLGLDVKVMD
                     EQDNEIEMTDVDDDDVVERKVDLQQNDAPETQKEVTD"
     gene            complement(19596..20204)
                     /locus_tag="POB55_000842"
     CDS             complement(19596..20204)
                     /locus_tag="POB55_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDI6499817.1"
                     /translation="MSHYYDEDPSVISNEQRIQYQLNHHKIDLITDNGVFSKDKVDYG
                     SDVLVQTFLKAHPPGPSKRIADVGCGYGPIGLMIAKVSPHHSITMLDVNHRALALVEK
                     NKKINGIDNAIVKESDALSAVEDKSFDFILTNPPIRAGKETVHRIFEQALHRLDSNGE
                     LFVVIQKKQGMPSAKKRMNELFGNVEVVNKDKGYYILRSIKA"
     gene            complement(20379..20747)
                     /gene="rplL"
                     /locus_tag="POB55_000843"
     CDS             complement(20379..20747)
                     /gene="rplL"
                     /locus_tag="POB55_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HDI6499818.1"
                     /translation="MANHEQIIEAIKEMSVLELNDLVKAIEEEFGVTAAAPVAVAGAA
                     GGADAAAEKTEFDVELTSAGSSKIKVVKAVKEATGLGLKDAKELVDGAPKVIKEALPK
                     EEAEKLKEQLEEVGATVELK"
     gene            complement(20790..21290)
                     /locus_tag="POB55_000844"
     CDS             complement(20790..21290)
                     /locus_tag="POB55_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HDI6499819.1"
                     /translation="MSAIIEAKKQLVDEIAEVLSNSVSTVIVDYRGLTVAEVTDLRSQ
                     LREAGVEYKVYKNTMVRRAAEKAGIEGLDEFLTGPTAIATSSEDAVAAAKVISGFAKD
                     HEALEIKSGVMEGNVITAEEVKTVGSLPSHDGLVSMLLSVLQAPVRNFAYAVKAIGEQ
                     KEESAE"
     misc_feature    complement(21328..>21461)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
BASE COUNT         6425 a         4222 c         3342 g         7472 t
ORIGIN      
        1 ataatgaata atattagaat tcatgcacta gtttattaaa ataaagagta atttaaaata
       61 tcattccgtg tattaaagtg aatggaaatg attagttatt atttttaaca gtatcttttt
      121 gttcaatagc ttctaacatt aatttagtca tgctcgctaa atcatattta ggatcaaatc
      181 cccattcgcc acgtgaacag cttgtatcaa tagaatccgg ccaactatta gcgatacctt
      241 gtctaatagg atcaacatcg taatctaatg taaaattggg atagtattct tgaattgctt
      301 cttttaccat ctctggatca aaactcattg cgctcaaatt ataaccattt ctagtttcta
      361 atttagcgtc gtctgcttcc ataagtttaa taattgcttc aattgcatca tccatataca
      421 tcatatccat atacgtgcct ttatctatga agcttgtata atgaccctct cttactgctt
      481 tgaagtatat ttcaacagca tagtctgtag taccgccacc tggctcttta acatgcgaga
      541 ttaaacctgg gaatctaaca cttcttgtat ctacaccaaa acgtttgaaa tagtattgac
      601 acaataattc tccagctact ttatttacac catacattgt cgtaggttgc tgaatcgtta
      661 cttgtggcgt attaacttta ggagttgagt ctccaaatgc accaattgaa cttggtgtga
      721 aaaagtgcaa attataagtt cttgcagctt ctaatgcatt cattaatcca cccatattta
      781 aatcccaagc tagaattgga tttttctcag cagttgctga taataatgct gccatatgca
      841 ttagactatc cgcttcaaag tccctaacta actcaaacat acggtcacga tctgttacgt
      901 ctaagatttc aaatggtcca ttttgtacag gtgagtctgc ttcaggttcc ctaatatctg
      961 tagcaagaac attatctgtc ccataaattt ctctgcactt aacaactaat tctgtaccaa
     1021 tttgtcctaa tgcaccagta atcataattt ttttcataga aatatctcct ttgtctcttc
     1081 tatatagcta tagtccatca caagcggaca taatattcat tttcataata attataatat
     1141 aaaagcgctt tcttgtatat atgacatgta catgttgctg atatttctgt aaatggaaat
     1201 tctagttgta ttaattgatt ttagtaattt atagcgttta ttattgctaa ttactgtcaa
     1261 attaaatttt ttatccctca actcttaaac tctggatatc tttcattata ttagcttttt
     1321 tataaccatg gatatcatgt aaagccttat aagctttaaa taatgtttca tacctttgta
     1381 ctttttccgc ttctggatta atttcgtata aaattggctc tttcatagca tcaactgcat
     1441 cattaattga attatgtgcg ccaccactga ctgcacctaa tattgctgcg cctattgctg
     1501 gtgcatactc actatccata acaattagtt ttttattcag tacattcgca tagatatcca
     1561 tcataacagc attcttttta ggtataccgc cacatgcaaa taccttttct acttccatat
     1621 gccaatcttg atactgttgc ataatcatct tagtaccaaa tgctgtagct tctaaatatg
     1681 ctctataaat atcttcatgc ttagtttgta aagttaatcc aaagatacaa cctgttaaat
     1741 tgctatcact taatacactt cgatttccat tatgccaatc aagagcaatg agcccacttt
     1801 cacctggcat ttgatgtttt atcttttcat tcattaattc aaatacagtc atatttctat
     1861 ttgctgcttc atctacatat gactttggtg cttgcttagc gacatactca aacaaatcac
     1921 ctactgctga ttgccccgct tcataagcaa ataattctgg aataattgct ccttttacag
     1981 aacctgatat acctggcact tgatgttgct tttcatttaa cataagatgg catgtgcttg
     2041 ttcccatcac catagtcatt tctttatctt tttcagaccc aatacctaat aaactagcat
     2101 gggcatcaat aataaaagga cttaccatag tctcttttga taatcctaat ttctgtgcca
     2161 ttttatcatc cagtttccct actgcttcac caatattaac aaccggtgca gatactttat
     2221 cttgaattac ttttgataat ttggggtcta ttttatcaaa taaatcataa tgaaaccctg
     2281 tttcttcttc ccaaaatgct ttgaaaccta atccacaatt cgagcgtata tttttattag
     2341 ttaatttatt tacaatccaa tcgcccgctt ccataatata agccgttttt tccataattt
     2401 ctggtgctcg attcatgact tccattattt tgggaatcat ccattcacta ctaacattat
     2461 atccataatg gcctaaccac ttattattat tttcaatagc agtttgatat aatttctctg
     2521 cttctttata tgcaccatga tgtttccaaa gtttcacata cgcatgtgga ttgtttttaa
     2581 attgttttaa attatgtacc gggttaagat tttcgtcagt aaaaataata gtagatgaag
     2641 taaagtctat accaatacct actatattgt ctggatctat ttttgattca cgtactatat
     2701 atgatattcc ttcttccata atttctaaat aatcattact attttgaagt gcatatgtat
     2761 gtggtatttt caaaccattt aattcacttt caatgacacc atgtgtatat ggtttcacaa
     2821 attttgatac tacttgaccg ttagttgtat taattaaaaa cacacggcct gaagctgttc
     2881 cataatcaat tccaatgcta taagacatca tgtttcctcc atatcgctag ttgcggttat
     2941 tagttttgca agcgttttct taaatcatac tcataatagg ttctatttta caagtatcac
     3001 ttaaattttg ccaaaaaaaa gagccctcat acatttacat atgaggctcc tctaattaat
     3061 atttaattat ttattttgta ttgcatttaa catttcatta actgctaatt ttcctaactc
     3121 atttgaagct aaaggactgt cacctgttaa taatttacga tcttttaacg ttcttcctgt
     3181 catatcgtcg ttaactactt ttaatccttg tttagttaat aaatcagcaa ctaaccattt
     3241 caagcgtcca ggtaaataac ctatttcaat atttgcacct tcatctaatg agtcagggaa
     3301 gacacaaaca gagtatcctt ctaatgtaga tttttctctg ttaagccctg ctgaaagtag
     3361 tgctgctggt ccatgacata atgtaactat aaagcggtca ttgtctaatg cccaatctaa
     3421 tgtttgttga acatcctcac tttcagaaat accaacaact gcagcatgtc cgcctgggat
     3481 aaagacagat aaataatctg aatcaggtcc taattcattt ttaatcacat ctgctaattt
     3541 ttttggctgt tttaattttt ctttcaattt attataagta cttataactg cctcgtcttc
     3601 agttggcata gcccataatt ctaatttaac tggataacca gataatgtcg caacatcaac
     3661 gtcaaaacct gcttccatta aatgatgtaa aggtaataac atttcaacag gatgattacc
     3721 cgtagagaac atttttccat tttccaataa tacatatctc tcttcagcag caatcatcaa
     3781 tactttccat ttaccatctt tataggcacc tttgtgttca acaccatcaa aatctgtttt
     3841 aggtgctgta tattgactaa gggaatatgg tgatgggaaa aatgcgttat cttctgcttt
     3901 atctggcgtt ggttgcttac ttaattcatt tacatcttgt gacatagttt attcctactt
     3961 tctctatata gttactacta gtatagacat atactacaca ctataaacat gtattactat
     4021 taaaatgctc attatcaaaa ataatcaaaa tgaatttctt tgaaaaaaat acaactaaat
     4081 tttacaagat gcttttaaaa ttaaaacttt aatttttggg agcactaaac attgttataa
     4141 gaacttactg ttaataaact aaacatttaa aaaaagccca cgacaatatt tgccgtggga
     4201 ataaataaat attaaatcaa cttcatttct tttcctactt tttcataagc cgcaattgct
     4261 tcatctaaca tgtcttttgt atgtgcagct gtaggcatat ttcttacacg tcctgtacct
     4321 cttggtactg ttgggaaaac gatagatttc acatagacac cttcgtcttt taaacgctta
     4381 ctaaattctt gagttgtttt ttcatcacca ataattactg gtgtaattgg agtttctgac
     4441 tcacctgtat catatcctaa ttttgacaat ccatttttta aatattgtgc attgtcccat
     4501 aatttatcat gtaattcagt tgaatccatt aactttttaa ctgcttcagt tattgctttg
     4561 gtatccccag gtgctaatga tgtagagaat aagaatggtc gtgattgtgc ttttaaccaa
     4621 tctattaact cttttgtacc tgctacataa ccgccaacga caccaattgc tttagaaagc
     4681 gtacctattt ggaaatcgat tttatcttgt aaaccaaaat gtttaaccgt accagcgcct
     4741 ttacccataa cacctgaacc atgagcgtcg tctacataag ttaataaacc aaattcttct
     4801 gcaatttcta caatttcagg taatttagcc acatcaccat ccatactaaa aacgccatca
     4861 gtgatataca tcactttatt gtattgacct gattcaactg cttcttttgc tttcgcacgt
     4921 aaatcatcca tgtctgaatg gttaactcga ataattttag ctttagataa gcgacatcca
     4981 tcaataattg atgcatgatt aagctcatct gataaaatag catcattttt attcatgaca
     5041 gctgaaatag cagccatatt acaattaaat cctgattgat aagctattgc agcttctgtt
     5101 cctttaaatt ttgctagtgt ttcttctaat tcgtcgtgta aatctaatgt accattgatt
     5161 gtacgaacag cgcctgcacc tactccatgt gtatcaatag ctgcttttgc agctgatttc
     5221 aaatcttcat ttgttgctag tcctaaataa ttatttgaag ataagttaat gtatgatttc
     5281 ccattgattt tgatttctgg tccgtttgca ccttcaattg tatctatttc attatacaaa
     5341 ccattttctt ttagataatt tatattttcc tctaaaaact catgtaatga ttgaaccaca
     5401 gtatataatc ccccttaagt attgtttggt aataccttta tcatacagta attctacata
     5461 acttgaaagc cttatcattc atagataaaa tatctcaaag caatcattta gtacgataat
     5521 ttcataaaat gttatactat aaacattatt ttagaaagta gtgataaggg tgttagattg
     5581 gttccaacta gcaaataata aagaaaataa aacaattcaa ctcagacgtt atttacacca
     5641 atatcctgag ttatcttttg aagaatttca aacacatgat tatattgtta accaattaag
     5701 ccagttatct tgtgatattg aaactcctat cggccgcaat ggtatcaaag ccacatttaa
     5761 aggcttgggc acaggaccta ccattgcatt aagagctgac tttgatgcgt tacctgtcga
     5821 ggaattaaac gatgtgcctt ataagtctaa aaatccagga tgtatgcatg cgtgtgggca
     5881 tgatggtcat acagcaattt tacttactgt agcagagatt ttagatgaac ataaacattt
     5941 attagaaggt aatgttgtat taatattcca gtacggtgaa gaaatcatgc caggtggttc
     6001 tcaagaaatg atagatgctg gatgtctgga aaatgtagat agaatttacg gtacacattt
     6061 atggagtggt tatcctactg gaactattca ttcacgtgcg ggggcaatca tggcctctcc
     6121 agatgaattt agtgtcacaa ttaaaggtcg tggcggtcat ggtgccaaac ctcatgaaac
     6181 cattgaccca atagtaatca tggcagagtt cattttaagt gcacaaaaaa taatttcacg
     6241 tacgatcgat ccagttaaac aagctgtact ttcatttgga atgattcaag caggcaccac
     6301 agatagcgtt attcctgatc aagcattttg taaaggtaca gttagaactt tcgattcaga
     6361 tatacagaat catgtaatgg acaaaatgga taagctttta caaggtttag caatagcaaa
     6421 tgatattaac tatgatttga attatattaa agggtatttg cctgtccata ataacgagaa
     6481 agcatatcaa gttattaaag aagcaacaaa tgacttacat gtacgcttta atgaatcaga
     6541 tttaatgatg attggagaag atttttcaca ttatttgaaa gtacgaccag gtgcattctt
     6601 tctgactggt tgtggtaatg aaagtaaagg tattacagcg ccacaccata atcccaaatt
     6661 tgatattgat gaaaaatcat taaaatacgc tgtagctgta tttttaaaaa tcattgaatt
     6721 agagcaagta tttaaaacaa attaagcatt taaattaatt gaaagatatg aatctgagac
     6781 ataaactaat gaaatgctcc cttcaaagta gacattgaaa aaatgaaaac tttgaaggga
     6841 gcattttaca atgtccaaga ctcaacaatt ttatttaaac agtattatca gttataagtt
     6901 ttattagtca gtctttcatc aattattgtt cttctaaatc gaatagccat tacaaaattt
     6961 acacattaaa aaaagacccc tcgattgagg gatcttcttt ttatataaaa tctaagaaat
     7021 tagaaattat ttaatgattt cagtaacaac gcctgatcct acagtacgtc caccttcacg
     7081 gattgagaaa cgagtaccgt cttcaatcgc gattggagcg attaattcta ctgtcatttc
     7141 aacgttatca ccaggcatta ccatttcagt accttctggt aagtgaacaa caccagttac
     7201 gtcagtagta cggaaataga attgtggacg atagtttgag aagaatggag tgtgacgtcc
     7261 accttcgtct tttgataata cgtatacttc tgctttgaat tcagtatgtg gtgtaattga
     7321 accaggagca gctaatactt gaccacgttg tacgtcttca cgagcaacac cacgtaataa
     7381 tgcaccaatg ttgtcaccag cttcagcgta gtctaataat ttacggaaca tttcaacacc
     7441 tgtaacagtt gttttagatg tgtcatgtaa accgatgatt tcaacttctt caccaacttt
     7501 gatttgacca cgttcaacac ggcctgtagc aacagtacca cgaccagtga ttgagaatac
     7561 gtcctcaact ggcatcatga atggtttgtc agaatcacgt tctggagttg gaatgtaagt
     7621 atctacagct tccattaatt ctaagatttt ttcttcgtat tgagcatcgc cttctaaagc
     7681 ttttaatgct gaaccagcga ttacaggtac atcgtcacct gggaagtcat attcgcttaa
     7741 taagtcacga acttccattt ctactaattc taataattct tcatcgtcaa ccatgtcaac
     7801 tttgtttaag aatactacta atgctggtac accaacgtta cgtgataaaa gaatgtgttc
     7861 acgagtttgt ggcattggac cgtcagcagc agatactact aagataccgc cgtccatttg
     7921 agcagcacca gtgatcatgt ttttaacgta gtcagcgtgt cctgggcagt caacgtgagc
     7981 gtagtgacgt ttgtcagttt ggtactcaat gtgagaagta ttgattgtga taccacgttc
     8041 tttttcttct ggagcgttgt caatcatgtc atatgattgt gcaactgagt caccattttt
     8101 tgctaataca gtagcgattg ctgctgttaa tgttgtttta ccatggtcaa cgtgaccgat
     8161 agtaccgata ttggcatgtt ctttagaacg atcgaatttt tcttttgcca ttataaaatc
     8221 tctcctattc taattaaaaa taatatttaa atttatctct catgatagtt tctcaccatc
     8281 atgagaaatt atttatagcg atgcaaaaag aattttacaa ttcttttata atgcattcac
     8341 cccaaaaaat caaactataa agatgatagc ttacatttaa gctcaccttg tattttaacc
     8401 taggctagct agtcaaaaca agttatatta ttcaccttta tttttcttga taatatcttc
     8461 agcgattgat tttggaactt cagcatagtg atcgaagtac atagtgtaag taccgcgacc
     8521 ttgagtgttt gaacgtaatg atgttgcata accgaacatt tctgaaagtg gtacataagc
     8581 attaacaact tgtgcattac cacgaggttc cataccatca acacgtccac gacgagatgt
     8641 tacgtcaccc atgatatcac ccatgtactc ttcaggcatt tcaatagtta ctttcatcat
     8701 tggttctaag attacaggat cacatttttt agcagcttct ttaagtgcta atgatgcagc
     8761 aattttgaag gccatttcag atgaatcgac atcatggtat gaaccatcat ataatttagc
     8821 tttaacatca attaaaggat aacctgctaa gacaccattt tccatagcat ctttaagacc
     8881 agcttctact gatggaatgt attcacgagg aactacacca ccaacgatag cgttttcgaa
     8941 ttcgaaacct gcgcctgttt cgtttggtgt gaattcaatg tgaacatcac cgtattgacc
     9001 acgaccacca gattgacgag agaatttacc ttgaacttgt gcagatgatt tgaatgtttc
     9061 acgatatgaa accattggag cacctacgtt acattcaacg ttgaattctt tcttcatacg
     9121 gtctactaag atgtctaagt gaagctcacc cataccaccg atgataactt gtccagtttc
     9181 ttcgtcagtg tgtgcatgga atgttgggtc ttcttcttgt aatttaacta aagcttgagt
     9241 cattttatct tggtcagctt tagattttgg ctctactgat aagtgaataa ctggctcagg
     9301 gaattccatt gattccaaga taatgtcatt tttctcacca cataaagtat caccagtacc
     9361 tgtatcttta agacctaccg cagcagcgat atctccagag tatacagtat cgatttcttg
     9421 acgtgagtta gcgtgcattt gtaataaacg acctacacgt tcacgtttac ctttagtaga
     9481 gttcttaacg tatgaaccag atgtcattgt acctgaatac acacggaaga atgtcaattt
     9541 accaacataa gggtcagtca taactttgaa cgctaatgca gcgaattcag ctgaatcgtc
     9601 tgctttcgcg attacttctt cttcagggtt gctagcacgg tgaccaataa ttggtttaac
     9661 gtctagtggt gaaggtaagt aatcaattac agcgtcaagc attaattgaa cacctttgtt
     9721 tttgaaagct gtaccacaaa gtactgggta gaattctacg ttagtagtag cttggcggat
     9781 agcttctttt aattcagaaa ctgaaatttc ttcgtcacca agatattttt ccattaattc
     9841 gtcgctagtt tctgcaactg cttcgattaa gctagcacga gcttcttcag ctctatctaa
     9901 gtggtcttca ggaatttcaa tttcttcaat ttcagtacct aaatcatttg tatatttgaa
     9961 acatttcatt tcaactaagt caatgattgc ttcgaattcg tcttccgcac caattggtaa
    10021 ttggattgga gcagcgttag cttgtaaacg atcatgtaat gtacttacag agtattcgaa
    10081 gttagcacct aatttgtcca ttttgtttac aaatacgata cgtggaacac cataagttgt
    10141 agcctgacgc caaactgttt cagtttgagg ttcaacacct gattgtgcat caagtactgt
    10201 aactgctccg tcaagtacac gtaatgaacg ttcaacttct acagtgaagt ctacgtgtcc
    10261 aggtgtatcg ataatgttta cacggtgacc ttcccaagct gctgttgttg cagcagatgt
    10321 gatagtaata ccacggtctt gttcttgctc catccagtcc atttgtgaag caccttcgtg
    10381 tgtttcacca attttgtgga tacggccagt gtaataaaga atacgttcag tcgtagtcgt
    10441 tttaccagca tcaatgtgag ccatgatacc gatattacga gttttttcta atgaaaattc
    10501 tctagccatg tattttttct ccttccagtt tatattactg aataaatacg atagatatgg
    10561 cgatgcccta agcatgaacg cttaatgaaa attcattaat atagaacaca ctcagggtaa
    10621 aagcttttat cttaccaacg gtagtgagca aatgctttgt ttgcttcagc cattttgtga
    10681 gtgtcctcac gtttcttaac ggcaccacct gtattatttg ctgcatctaa aatttcgtta
    10741 gctaaacgat cttccatcgt tttttcacca cgaagacgtg catagttaac taaccaacgt
    10801 aaacctaaag tagtacgacg ctctggacga acttctactg gtacttgata gttagaacca
    10861 cctacacgac gagctttaac ttctaatact ggcataatgt tgttgattgc ttcttcgaat
    10921 acttctaatg catcacgacc actgcgttgt tcaactaggt cgaatgctga ataaagaatt
    10981 ctttgtgctg ttccacgttt accatctaac ataattttgt taattaattt agttactaac
    11041 ttagagttat gaattggatc tggtaatacg tctcttttag gtactgatcc tttacgaggc
    11101 ataatgtaaa tcctcccttc cttatattaa atattttaag tttaagattt aattaaaaac
    11161 taaattctta gtttttaggt ttcttagttc cgtataatga acgaccttgt ctacgtccgt
    11221 caacacctga agtatcaagt gctccacgta caatatggta acgcacacct ggtaagtctt
    11281 ttacacgtcc accacgtaca agtacaacac tgtgttcttg taagttatgg ccgataccag
    11341 ggatgtatgc gttaatttcg atgttgtttg ataaacgcac acgtgcatat ttacgtaacg
    11401 ctgagttagg ttttttaggt gtcattgtac ctacacgagt acatacacca cgtttttgtg
    11461 gtgagtttaa gtcagtaaat tttttctttt tactgttgaa acctttattt aaagctggag
    11521 aatctgattt tttgattttg ctttgtcttg gtttacgtac taattggtta atagttggca
    11581 tgtgatatgt cctcctctct tcacttttta atcccacaca tccaggtggt tcatttttag
    11641 gttaaataaa atttagtaag taaaacactt actaattctc atttaatcaa tgctactatt
    11701 gtcgcattga cgttaatacc tacatgttta cccaaagcat gtttgctttt gaaaaaagat
    11761 acaggtatat ttttctgatt gatttggctt aacacgcgag tcattaaata tacttcaacg
    11821 tcttcagcaa taatcaaaga tgtaacttga tctttcttta acgctttaag cgtttcttta
    11881 agaccaacta caaaatgttg tttgttaaag cgtgcaactt tttccttaga caataattta
    11941 tcctccaaag ttctgcttgc atcaaccttt aatatattaa cattgaacaa gagaattcgt
    12001 caacattaaa tcaaataatt tacataaaaa taacaagagg ctaaagcatt agcctctgtt
    12061 atatacttgt tattccgtta cttcagtttg agattcaact tctgcaactg gtttagctgt
    12121 tttttcgtat tttacgtcgc tataacgtct cataccagta ccagctggaa ttaacttacc
    12181 aataattacg ttttctttaa gacctaataa gtcatcacgc ttacctttaa ttgctgcatc
    12241 tgtaagaact cttgttgttt cttggaatga tgctgcagat aagaaacttt ctgtttcaag
    12301 tgatgcttta gtaataccaa gtaatactgg tttagctgtt gcaggacgct tacggtgttt
    12361 aaatgcttct ctatttgcat ctgtaaagtt atgaatatca actaatgaac ctggtaataa
    12421 cttcgtatca cctgcttcga taattctaac tttacgtaac atttgtcgaa ccataacctc
    12481 aacgtgttta tcgtcgattt ctacaccttg catacggtaa actttttgta cttcttttaa
    12541 taagtagctt tcagtcgcgt ttaatccagc aacagataag taattcttag gttcaataga
    12601 accttcagtt aatacttcac cacgttgaac tggttgaccg atttctacaa taattcttga
    12661 agtacctgaa gcaaggtatg atcttgtttc attagcacct ttaacaacaa tttcttgttg
    12721 tctatctttt gctaatttaa tatcttctac gacaccttcg atttccgtaa ttaccgcttg
    12781 acctttaggg ttacgtgctt cgaaaatctc ttgaatacga ggaagacctt gtgtgatatc
    12841 gctacctgct accccacctg tatggaatgt acgcattgta agctgtgtac ctggttcacc
    12901 gatagattgg gctgcaattg taccaactgc ttcaccaact tcaacttttt caccagtagc
    12961 aaggttttta ccgtaacatt tttcacaaac accatgtcgt gcgttacaag taaatgctga
    13021 gcgaatatac atttgttcaa taccagcatc tgtaattttc ttagcaattt caggtgtaat
    13081 taattcatca ggacgaatga ttatttcatc agtttcagga tgacgaattg tttctttaga
    13141 ataacgacct tcaatacgtt cgataaatgg ttcaatcatt tctgtacctt ctttaatatc
    13201 agaaactaat aaacctctat cagtaccaca gtcttcttca cgaacaataa catcttgtgc
    13261 cacgtcaaca agacgacgag taagatatcc tgagtcagct gttttaagtg ctgtatcggc
    13321 aagaccttta cgtgcaccgt gagttgagat gaagtattca agtactgtta aaccttcacg
    13381 gaatgaagat gtgattggta attcgataat cttaccagat ggtgcggcca tcaatccacg
    13441 catacctgct aactgtgtaa agttagatgc gttaccacgg gcacctgaat cactcatcat
    13501 gaagattggg ttagttttat caagtgattg catcaattca ccttgaattt gatcttttgc
    13561 atctgtccaa atttcaacaa ctgcattata tctttcttct tcagtgatta aaccacggtt
    13621 gaattgtttt gtaattctgt cgactaattt ttcatgctca tcaagtattt gttgcttatc
    13681 aggtaatact acgatatcag caacacctac tgtaatacca gctttagatg agaatttgaa
    13741 tcctaagtct ttcatacggt ctaacatcat tgatgtatca gtgatgctaa atctgttgaa
    13801 tacttctgca ataatattac ctaagaattt tttgttgaaa ggttcaatta attcttcatt
    13861 ttcaaagtat tcttttaatc caccttcacc taaagttgta ggatcgatga aatatctgtt
    13921 tggtgtcttt ctttctaagt tttcttgcgt aggttcatta atataagcaa atgaatctgg
    13981 aatgatttca ttgaatataa ttttacctac tgacgtagca agaatctttt tgttttgttc
    14041 ttcagtaaat gttggattat tgaacgaact tgcatgtaca ccaattctag tgtgtaaatg
    14101 tacaaagcca tttgcatatg cttttaatac ttcatttgta ttattaaaga ttgcgcctgt
    14161 atttactgca tcttttcttt ctaaagtaag gtaatagtta ccaagtacca tatcttgtga
    14221 tggtgtaact acaggtttac catctttagg gttcaagatg ttttgtgctg ctaacatcaa
    14281 cattcttgct tcagcttgtg cctcttttga taaaggaacg tgaaccgcca tttggtcacc
    14341 gtcaaagtca gcgttataag ctgttgttac aagtggatgt agacgaatcg cacgaccttc
    14401 aactaaagtt ggttcaaatg cttgaatacc aagtctatga agtgttggtg cacggttaag
    14461 taatacagga tgttctctaa ttacttcttc caatacgtcc caaacttcat catccatgcg
    14521 ttcgatttta ctcttcgcat ttttaatgtt agttgcaatt tcacgttgaa ctaattcttt
    14581 cattacgaat ggtttaaata gttcaagtgc catttctttt ggcaaaccac attggtacat
    14641 tttcaagctt ggacctactg caataactga acgtcctgaa tagtcaacac gtttaccaag
    14701 taagttttga cggaaacgac cttgtttacc ttttaacata tgagataaag attttaatgg
    14761 acggttacct gggccagtaa ctggacgacc acgacgacca ttatcaatta aagcgtcaac
    14821 ggcttcttgt aacatacgtt tttcgttttg aacgatgata ccaggtgcac ctaaatctaa
    14881 taaacgtttc aaacgattat ttcgattaat tacacgacgg tataaatcgt ttaagtcact
    14941 tgttgcaaat cgtccaccat ctaattgaac cattggacga atttctggtg ggatgattgg
    15001 aagtacatct aaaatcatcc atgaaggttt gttacctgaa ttacggaatg attcaacaac
    15061 ttctaaacgt ttaattgcac gagtaagtct ttgaccagta gctgattcca actcatcgcg
    15121 taacaattta agttcttcgt caagatcaat ctcttcaagt aaatctttaa taccttctgc
    15181 acccattttt gcaacgaatt gacctgggta tttatcataa taatctctga attcagcttc
    15241 agataataaa gttttctttt ctaaaccagt tggacctgga tctacaacaa cataagaagc
    15301 aaagtaaata acttcttcta atgctcttgg tgacatgtca agtaataatc ccatacggct
    15361 tggtatacct ttgaaatacc aaatgtgaga aactggagca gcaagttcaa tgtgacccat
    15421 tctttcacga cgtactttag atttagttac ttcaactcca catctgtcac agaccatgcc
    15481 tttgtagcga acacgtttgt atttaccaca actacattcc cagtcttttg taggtccgaa
    15541 aatcctttca cagaatagac catctttttc aggttttaat gtacggtagt tgattgtttc
    15601 aggtttttta acttcaccaa aagaccaaga acggattttt tcaggtgaag ccaatcctat
    15661 tttcatataa tggaaattat ttacatcaat caaggagcct acctccttca atttagatta
    15721 gctgtgctat ttgattgatt tgcatttatt taaaatggct taaagtacta aactgaatca
    15781 tcaataaaat tcagcttagt atctttttgc ctgttttgta aattgcgtat taatcagtaa
    15841 cttctttttg tgtttcagga gcatcatttt gttgtaaatc tactttacgt tctacaacat
    15901 catcgtcatc aacgtctgtc atttcgattt cattatcttg ctcatccata acttttacat
    15961 ctaaacctaa actttgtaat tctttcatca atactcggaa tgattctgga acacttggtc
    16021 tagagatgtt ttcaccttta acaatagcct cgtatgtttt cacacgtcct actgtatcat
    16081 cggatttgta agttaagatt tcttgtaatg tgtatgcagc accatatgct tcaagtgccc
    16141 atacctccat ctcaccgaaa cgttgtccac cgaattgcgc tttacctcca agtggttgtt
    16201 gtgtaacaag tgaatatggt cctgttgaac gcgcatgtaa tttatcatca accatgtgcg
    16261 caagtttcaa catgtacatt acacctactg aaatacggtt atcgaatggt tcacctgtac
    16321 gtccatcata aagtacagtt ttaccatcac gagccatacc agcttcttca attgttgacc
    16381 atacatcgtc atcgtttgcg ccgtcaaata ctggtgatgc aacgtgaata ccaagatttt
    16441 tagcagccat acctaagtgt agctctaata cttgtccgat gttcatacga gatggtacac
    16501 caagagggtt taacatgata tcgatcggac gtccgtctgg taagtaaggc atatcttctt
    16561 caggaacaat cttagaaatg acacctttgt taccatgtcg accacacatc ttatcaccaa
    16621 catgaatttt acgtttttga acgatgtata cacgtactaa ttggtttaca ccaggtgata
    16681 atgtatcgtc gccttcttcg cgattgaata cttttacatc aagaacgata ccgccagcgc
    16741 cgtgaggtac acgtaatgaa gtatctctaa cttcacgtgc tttttcacca aagattgcat
    16801 gtaacaatct ttcttcggca gttaactcag ttacaccttt aggcgttact ttaccaacta
    16861 aaatatctcc atcttttact tctgcaccaa tataaacgat accacgatcg tctaagttct
    16921 taagtgcact ttcagaaaca ttaggaatat ctcttgtgat ttcttcaggt cctaacttag
    16981 tatcacgtgc ttctgattca tactcttcaa tatgaataga agtatacacg tcatctttca
    17041 caagtctttc actcatgata acggcatcct catagttgta accgtcccaa gtcatgaaac
    17101 caactactac gtttctacct aatgccattt ctcctaattc catagatggt ccatctgcta
    17161 aaatctcgtt atactcaaca acatctccaa ctgcaacgat tggacgttgg ttgtaacatg
    17221 tacctgagtt tgaacgttta aatttagcta atggatagcg atctaattca ccttcatgct
    17281 caacgccgtt ctcttcaact agacgacgta caagaatttc attagattca acatgttcaa
    17341 cacgacctct gtgcttagct gtaatagctg caccagaatc acgtgctgca acgtgttcca
    17401 tacctgtacc aacaaatggt gcttctggat tcatcaaagg cactgcttga cgttgcatgt
    17461 tcgcacccat caatgcacgg tttgagtcat cattttctaa gaatggaata catgctgtcg
    17521 ctgctgaaac aacttgcttc ggcgatacat ccatataatc cattttttct ttagccataa
    17581 ctgtgttgtt accacggaaa cgacatacaa cttcatcatc catgaaacga ccattttcat
    17641 ctaatttaga gtttgcttgt gctacaacat agctatcttc ttcgtcagct gttaaatagt
    17701 caatttgatc agtgatagca tgtgtatcta aatcaacttt acgatatggt gtttcaataa
    17761 agccgaattc atttacacgt gcataacttg ataatgagtt aatcaatcca atgtttggtc
    17821 cctcaggtgt ttcaattgga cacatacggc catagtgaga gtagtgaacg tcacgtactt
    17881 ccatttgagc acgttcacgt gttaaaccac caggtcctaa tgctgataga cgacgtttat
    17941 gcgttaactc agctaatgga tttgcttggt ccatgaattg tgataattga gagctaccaa
    18001 agaattcttt aatagatgca ataacaggtc gaatattaat taattgttga ggtgtgatag
    18061 actcagtatc ttgaattgac attctttcac gtacaactct ttccattctt gataaaccga
    18121 tacggaattg gttttgtagt aattcaccta cagaacgtaa acgacggtta cctaaatggt
    18181 caatatcatc tgtatatcca ataccgctta ataagttaaa gaagtaactc attgaagcaa
    18241 tgatatctgc tggtgtaatg catttaactt ctgagtcagg gaaagcatta ccaattacag
    18301 ttgtcgtacg accttcatca tcgttaggaa catatacttt aattgattga atttctactg
    18361 gctcgtctat aacgctacca tgcaattcaa acacttcgct gtttgcattt gattcaagta
    18421 catccatgat ttcgtcgatt ttacgacgat caagcactgt accttcttca actacaattt
    18481 caccagtttc agtatttaca attggctcag ctaatttttg attaaataaa cgatgtttta
    18541 aatgtaattt tttgtttgtt ttataacgac ccacgcttgc taagtcatag cgttttggat
    18601 caaagaaacg tgaatacaat agacttttag cattttcaac agttggtggt tcacctggac
    18661 gtaaacgttc atagatttct aataacgctt gttcagtgtt ttcagtgccg tctttctcta
    18721 aagtattacg taaatattca ttgtcaccta aaaggtcaac aatttcttgg tcgcttgaga
    18781 aacctaatgc acgtaacaat actgttaatg gtagtttacg tgttctatca atacgtacgt
    18841 atacaacatc tttagcatct gtttcatatt ctaaccatgc accacggttt ggaataattg
    18901 ttgcatcata gttttcacga ccatttttgt cgattttttc attgaaataa acggatggtg
    18961 aacgaactaa ttgagatacg attacacgtt ctgcaccatt gataacgaac gtacctgtat
    19021 cagtcattaa tgggaaatca cccataaaga cttcttgttc tttaacttct cctgtttctt
    19081 taatgattag acgcactttt acacgaagag gtgcagcata agtagcgtca cggtttttag
    19141 attcttctaa atcatatttt ggttctccta aacggtaatc cacaaactct aatgacaaat
    19201 taccagtaaa atcttcaatt ggagaaatgt ctctaaacat ttcgattaaa ccttctctta
    19261 ggaaccactc gtaagattta gtttgaattt ctattaagtt tggtaattct aatacttctg
    19321 aaattctcgc gtagtttcta cgtttacgat gtcttccata ttggacaact tgacctgcca
    19381 aacagattca cccctcaaaa attatgtgat ccgcttttag ctaaatacca taagcatatt
    19441 aataaaaaga caaaaagaaa acggcaacac taatgatgac accattttcg attctatatt
    19501 tattcttaat taacttatct atttaacgta aaagtacaca cttattgaac ataaattttt
    19561 acattatata actataacag aatatccatt tcatttcaag cttttatact tctcagaatg
    19621 taatatcctt tatctttatt taccacttct acatttccaa aaagttcatt cattcttttc
    19681 tttgcagatg gcataccttg cttcttctga attacaacga atagttcacc gttcgagtct
    19741 aatctatgta atgcttgctc gaatatacga tgcacggttt ctttccctgc tcttattggt
    19801 ggattggtta aaataaaatc aaaacttttg tcttccacag cagacaaagc atcactttcc
    19861 tttacgatcg cattatcaat accatttatt tttttgtttt tttcaactaa ggctagcgct
    19921 ctgtgattaa catctagcat tgtaattgaa tgatgtggtg atactttagc aatcatcaaa
    19981 ccaattggtc cgtaaccaca accaacatcg gcaattcgct tacttggacc aggtggatgc
    20041 gcttttaaaa aagtttgaac aagaacatct gaaccataat ctactttatc tttcgaaaac
    20101 actccattat cagttattaa atcaatttta tgatggttta attgatattg aatacgttgt
    20161 tcattgctaa ttacacttgg atcttcatcg taataatgac tcattattcc acctcatctt
    20221 cactttgtaa ctcgtcatta taattataac atttcactca gtctttaaat gaataaaacc
    20281 ccgttatcaa aataacgggg ttttaacttc tcaaagaatc attatattat agcaatactt
    20341 gctataaaac tttgattatt aagtttaaga tacttgaatt attttaattc tacagtagct
    20401 ccaacttctt ctaattgttc tttaagtttt tcagcttctt ctttaggtaa agcttctttg
    20461 attactttag gagctccgtc tactaattct ttagcatctt ttaatcctaa accagttgct
    20521 tctttaacag ctttaacaac tttgatttta gatgaaccag ctgaagttaa ctcaacgtca
    20581 aattcagttt tttctgctgc agcgtcagcg ccaccagctg cacctgctac tgctactgga
    20641 gcagctgcag ttacaccaaa ttcttcttca attgctttta ctaagtcgtt taattctaat
    20701 actgacattt ctttaatcgc ttcaatgatt tgttcatgat tagccatttt ataattcctc
    20761 cattattttt aattttttta cgctaaaaat tattcagcgc tttcttcttt ttgttctcca
    20821 atagctttaa ccgcataagc gaagttgcgt acaggagctt gtaatactga taaaagcata
    20881 gatacaagac catcgtgtga aggtaatgaa ccaacagttt taacttcttc tgctgtaata
    20941 acattgcctt ccataacgcc tgatttaatt tctaatgctt catgatcttt agcaaatcca
    21001 gaaattactt tcgctgcagc tacagcatct tcacttgaag ttgcaatagc agtaggacct
    21061 gttaagaatt catctaagcc ttcgatacca gctttttcag ctgcacgacg taccatagtg
    21121 tttttgtata ctttatactc aacaccagct tcacgtaatt gtgaacgtaa gtcagtaact
    21181 tcagctactg ttaatccacg gtagtcaacg attactgttg aaactgaatt tgatagtacc
    21241 tcagcaattt catcaactag ttgtttttta gcttcaatga tagcagacat tcagacacct
    21301 ccatttaaat ttttggtgct ttttttaaaa ttaataaaaa aagcactttc tacccacggc
    21361 aaaaagtgct tgaaagatca tagatcatca cgttcaagtc aattttagcc tcggcaggat
    21421 aaattttaag ttataaataa ctcctactgt cttaggtaaa a
//
LOCUS       DAMZKC010000030        21146 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.030, whole genome shotgun sequence.
ACCESSION   DAMZKC010000030 DAMZKC010000000
VERSION     DAMZKC010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 21146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21146
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.030"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..1329
                     /locus_tag="POB55_000845"
     CDS             <1..1329
                     /locus_tag="POB55_000845"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HDI6499820.1"
                     /translation="YLSVSALTKYIKYKFDQDPHLQSVLIKGELSNFKKHSSGHLYFN
                     VKDKESVISAMMFKGSASKLNFEPKEGDEVLLEARVSVFERRGNYQIYVNKMQLDGIG
                     NLYQKLEALKKKLTEEGCFDKANKKSIPKFPKKIAVLTASTGAAIRDIHSTINSRFPL
                     AEQIQISTLVQGEKAKDDIIEKIEYADSLGVDTIIVGRGGGSIEDLWNFNEEAVVRAI
                     YNCKTPIISAVGHETDFTLSDFAADIRAATPTQAAVIATPDQYELLQQIQQYQFTLTR
                     FIKKHLEQQRKHVEHLSSYYKFKQPTLLYDQQIQRRDDLEKRLKQQIQATFEQQRHRL
                     MLLQQRYNLKALLSSVNQEQQNNLQLTNQLVKLLNSKILSYKNDLKNKVENLNNLSPT
                     NTMLRGYAIVNKKDEVITSTKDLTENDQLTLTMKDGLVDAKVTKVRCNND"
     gene            1322..1552
                     /locus_tag="POB55_000846"
     CDS             1322..1552
                     /locus_tag="POB55_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HDI6499821.1"
                     /translation="MTKETQSFEEMMQELEQIVQKLDNETVSLEESLDLYQRGMKLSA
                     ACDTTLKNAEKKVNDLIKEEAEDVKNDESTDE"
     gene            1530..2411
                     /locus_tag="POB55_000847"
     CDS             1530..2411
                     /locus_tag="POB55_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDI6499822.1"
                     /translation="MTNLPMNKLIDEVNNELSVAINKSVMDTQLEESMLYSLNAGGKR
                     IRPVLLLLTLDSLNTEYELGMKSAIALEMIHTYSLIHDDLPAMDNDDYRRGKLTNHKV
                     YGEWTAILAGDALLTKAFELISSDDRLTDEVKIKVLQRLSIASGHVGMVGGQMLDMQS
                     EGQPIDLETLEMIHKTKTGALLTFAVMSAADIANVDDATKEHLESYSYHLGMMFQIKD
                     DLLDCYGDEAKLGKKVGSDLENNKSTYVSLLGKDGAEDKLTYHRDAAVDELTQIDEQF
                     NTKHLLEIVDLFYSRDH"
     gene            2843..3295
                     /gene="argR"
                     /locus_tag="POB55_000848"
     CDS             2843..3295
                     /gene="argR"
                     /locus_tag="POB55_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HDI6499823.1"
                     /translation="MPKKSVRHIKIREIISNEQIETQDELVKRLNDYDLNVTQATVSR
                     DIKELQLIKVPIPSGQYVYSLPNDRKFHPLEKLGRYLMDSFVNIDGTDNLLVLKTLPG
                     NAQSIGAILDQINWEEVLGTICGDDTCLIICRSKEASDEIKSRIFNLL"
     gene            3311..4990
                     /gene="recN"
                     /locus_tag="POB55_000849"
     CDS             3311..4990
                     /gene="recN"
                     /locus_tag="POB55_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HDI6499824.1"
                     /translation="MLQTLSIKQFAIIEELEIQFSDGLTVLSGETGSGKSIIIDAIGQ
                     LIGMRASSDFVRHGEKKAVIEGIFDIDESKDAIHILKNMDIDVDEDFLLVKREIFSSG
                     KSLCKINNQTVTLQDLRKVMQELLDIHGQHETQSLLKQKYHLTLLDNYAESRYQDLLD
                     KYHQTFQNYKAKKQELEDLESADQALLQRLDLMKFQLEELSEAHLKEGEIEQLEIDIK
                     RIQNSEKLSLALNNAHMTLTDENAITDRLYELSNHLLTINDIVPNKYDKLKEDIDQFY
                     YILEDAKHELYDEMANTEFDEQVLNEYESRMNLLNNLKRKYGKDISELIAYQEKLNNE
                     INKIENYEQSTSQLREEINALYNQVIEVGQALSKQRRIVARELRDHIVSEIQNLQMKD
                     ANLEISFKKLEEPNIDGIEFVEFLISPNKGEPLKSLNKIASGGELSRIMLALKSIFVK
                     SRGQTAILFDEVDSGVSGQAAQKMAEKMRDIAEYIQVICISHLPQVASMSDHHLLISK
                     SSKDDRTTTQVQELISDDKVDEIARMISGASVTDLTRENAREMIQHNQRRR"
     gene            5141..6562
                     /gene="lpdA"
                     /locus_tag="POB55_000850"
     CDS             5141..6562
                     /gene="lpdA"
                     /locus_tag="POB55_000850"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDI6499825.1"
                     /translation="MSEKQYDLVVLGGGTAGYVAAIRASQLGKKVAIVERQLLGGTCL
                     HKGCIPTKSLLKSAEVFQTVKQAAMFGVDVKDANVNFENMLARKEDIINQMYQGVKHL
                     MQHNHIDIYNGTGRILGTSIFSPQSGTISVEYEDGESDLLPNQFVLIATGSSPAELPF
                     LSFDHDKILSSDDILSLKTLPSSIGIIGGGVIGMEFASLMIDLGVDVTVIEAGERILP
                     TESKQASQLLKKSLSARGVKFYEGIKLSENDINVNEDGVTFEISSDIIKVDKVLLSIG
                     RKPNTSDIGLNNTKIKLSTSGHILTNEFQQTEDKHIYAAGDCIGKLQLAHVGSKEGVV
                     AVDHMFEGNPIPVNYNMMPKCIYSQPEIASIGLNIEQAKAEGMKVKSFKVPFKAIGKA
                     VIDSHDTNEGYSEMVIDQSTEEIVGINMIGPHVTELINEASLLQFMNGSALELGLTTH
                     AHPSISEVLMELGLKAESRAIHV"
     gene            6578..7570
                     /locus_tag="POB55_000851"
     CDS             6578..7570
                     /locus_tag="POB55_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HDI6499826.1"
                     /translation="MIDYKSLGLSEEDLKVIYKWMDLGRKIDERLWLLNRAGKIPFVV
                     SGQGQEATQIGMAYALEEGDITAPYYRDLAFVTYMGISAYDTFLSAFGKKDDVNSGGK
                     QMPSHFSSRAKNILSQSSPVATQIPHAVGAALALKMDGKKKIATATVGEGSSNQGDFH
                     EGLNFAGVHKLPFVCVIINNKYAISVPDSLQYAAEKLSDRALGYGIHGEQVDGNDPLA
                     MYKAMKEARDRAISGQGSTLIEAVTSRMTAHSSDDDDQYRTKEEREALKKADCNEKFK
                     KELLSAGIIDDAWLAEIEAEHKDIINKATKAAEDAPYPSVEEAYAFVYEEGSLN"
     gene            7570..8553
                     /locus_tag="POB55_000852"
     CDS             7570..8553
                     /locus_tag="POB55_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HDI6499827.1"
                     /translation="MAKLSYLEAIRQAQDLALQQNKDVFILGEDVGKKGGVFGTTQGL
                     QQQYGEDRVIDTPLAESNIVGTAIGAAMVGKRPIAEIQFADFILPATNQIISEAAKMR
                     YRSNNDWQCPLTIRAPFGGGVHGGLYHSQSIESIFASSPGLTIVIPSTPYDAKGLLLS
                     SIESNDPVLYFEHKKAYRFLKEEVPEEYYTVPLGKADVKREGEDLTVFCYGLMVNYCL
                     QAADILAADGINVEVVDLRTVYPLDKETIIDRAKNTGKVLLVTEDNLEGSIMSEVSAI
                     IAEHCLFDLDAPIMRLAAPDVPSMPFSPVLENEIMMNPEKILNKMRELAEF"
     gene            8566..9840
                     /locus_tag="POB55_000853"
     CDS             8566..9840
                     /locus_tag="POB55_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HDI6499828.1"
                     /translation="MEITMPKLGESVHEGTIEQWLVSVGDHIDEYEPLCEVITDKVTA
                     EVPSTISGTITEILVEAGQTVAIDTIICKIETADEKTNETTEEIQAKVDEHTQKSTKK
                     ASATVEQTFTAKQNQPRNNGRFSPVVFKLASEHDIDLSQVVGSGFEGRVTKKDIMSVI
                     ENGGTTAQSDKQVQTKSTSVDTSSNQSSEDNSENSTIPVNGVRKAIAQNMVNSVTEIP
                     HAWMMIEVDATNLVNTRNHYKNSFKNKEGYNLTFFAFFVKAVADALKAYPLLNSSWQG
                     NEIVLHKDINISIAVADENKLYVPVIKHADEKSIKGIAREINTLATKARNKQLTAEDM
                     QGGTFTVNNTGTFGSVSSMGIINHPQAAILQVESIVKKPVVINDMIAIRNMVNLCISI
                     DHRILDGLQTGKFMNHIKQRIEQYTLENTNIY"
     gene            10191..10628
                     /locus_tag="POB55_000854"
     CDS             10191..10628
                     /locus_tag="POB55_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HDI6499829.1"
                     /translation="MDMNFDLYMNGVVEQARNEIESAGYEQLTTAEDVDKVLKQDGTT
                     LVMINSVCGCAGGIARPAASHALHYDVLPDRLVTVFAGQDKEATQRAREYFEGYAPSS
                     PSFALVKDGKITEMIERHQIEGHDVMNVINQLQTLFNKYCEER"
     gene            10642..11622
                     /locus_tag="POB55_000855"
     CDS             10642..11622
                     /locus_tag="POB55_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HDI6499830.1"
                     /translation="MLKLNPYKIGFRTIKTAVGMTLGVIISKLLGLDNYASSAILVVL
                     CIKHTKVHSLQAIISRLVSCFLVLFLGSAIFSLLGQSPIVLGIIVLLFIPLTVVLKVQ
                     EGVITSCVILLHVFNAKSIDAHLIVNETLLLLIGLSIAFTMNLMMPSLDKQLDEYKCK
                     IEQQIADIFSKYSYICEKYEDTIAIEFEVLLLNIKKAKSIAFRDVKNHFVRNENSYYH
                     YFDMREEQVELLMRMKPLIESICHKDPLLNDVASLLKEIGLNVNSNDYTAMRLHNLYE
                     TRLKLDELPLPTTHKALNSRASIIQILNELEEYLQIKSQFGSLKMHSEVA"
     gene            complement(11749..11928)
                     /gene="prli42"
                     /locus_tag="POB55_000856"
     CDS             complement(11749..11928)
                     /gene="prli42"
                     /locus_tag="POB55_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stressosome-associated protein Prli42"
                     /protein_id="HDI6499831.1"
                     /translation="MLVISSQVKSFISYVVIINTKVIHLEAHKMQNKVLRIIIIVMLV
                     SVVLALLLTSIIPIL"
     gene            12191..13324
                     /locus_tag="POB55_000857"
     CDS             12191..13324
                     /locus_tag="POB55_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HDI6499832.1"
                     /translation="MINEQRLLNTFFELVQIDSETGNESTIQPILKEKFIALGLDVKE
                     DEAAKHPKLGANNLVCTMNSTIEEGEVPKLYLTSHMDTVVPAINVKPIVKDDGYIYSD
                     GTTILGADDKAGLAAMLEVLQVIKEQQIPHGQIQFVITVGEESGLIGAKELNSELLDA
                     DFGYAIDASADVGTTVVGAPTQMLISAKIFGKTAHASTPKEGVSAINIAAKAISRMKL
                     GQVDEITTANIGKFHGGSATNIVADEVILEAEARSHDPERIKTQVKHMTDVFETTASE
                     LGGKAEVTVEQSYPGFKINDNEAVVKIAQESARNLGLSANTIISGGGSDGSIINTFGI
                     PSVILGVGYEKIHTTNERMPIKSLNLLASQVLEIIKIVARQSK"
     gene            13392..14798
                     /gene="gndA"
                     /locus_tag="POB55_000858"
     CDS             13392..14798
                     /gene="gndA"
                     /locus_tag="POB55_000858"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HDI6499833.1"
                     /translation="MTQQIGVIGLAVMGKNLAWNIESRGYSVSVFNRSSEKTDLMVEE
                     SKGKNIHPTYSLEEFVNSLEKPRKILLMVQAGKATDATIDSLLPLLDDGDILIDGGNT
                     NYQDTIRRNKALAQSAINFIGMGVSGGEIGALTGPSLMPGGQEEAYNKVADILDAIAA
                     KAKDGASCVTYIGPNGAGHYVKMVHNGIEYADMQLIAESYAMMKELLGMSHEDIAQTF
                     KDWNAGELESYLIEITGDIFMKLDENKEALVEKILDTAGQKGTGKWTSINALELGIPL
                     TIITESVFARFISSIKEERVNASKELNGPKASFDGDKKDFLEKIRKALYMSKICSYAQ
                     GFAQMRKASEDNEWNLKLGDLAMIWREGCIIRAQFLQKIKDAYDNNPGLQNLLLDPYF
                     KNIVTEYQDALRDVVATGVQNGVPTPGFSSSINYYDSYRAADLPANLIQAQRDYFGAH
                     TYERKDKEGVFHTQWIEE"
     gene            15017..15388
                     /locus_tag="POB55_000859"
     CDS             15017..15388
                     /locus_tag="POB55_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDI6499834.1"
                     /translation="MEKLNQVMLYVDDVDKAKAFWTETLEFVVVSETPLAEDYVAVEV
                     SPTKDAETSLTIMAKEFIEKYSPEVNLGTPSLMFKEKNFDALYSKLNDLGLTGHDIVE
                     MNGQRVFNFQDGQGNYFAVSD"
     gene            15327..15581
                     /locus_tag="POB55_000860"
     CDS             15327..15581
                     /locus_tag="POB55_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499835.1"
                     /translation="MVNVYLIFKMAKVTILQLVTKQSILIFIQTYILIFGLNFSMKVL
                     FLSFYAIVCTNLLTFLESVTKLVLYSGWKRFPMQKLTGGD"
     gene            15582..16601
                     /locus_tag="POB55_000861"
     CDS             15582..16601
                     /locus_tag="POB55_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDI6499836.1"
                     /translation="MVTIKDVALKAGVSPSTVSRVIKGNQRISEATISKVKKVMEELN
                     YFPNTAARTLITNQTYKIGLVLKGSEEPIRLNPFYINVLLGISETCNQHGYGTQTTVS
                     NNMNDLMDEVYKMIKQRMVDAFILLYSKENDPIKQMLIDESMPFIVIGKPTSDIDHQF
                     THIDNDNILASENLTRHVIEQGVDELIFITEKGNFEVSKDRIQGFETVASQFNLDYQI
                     IETSNERKVIFNYMQNLHTRLKDPNIKQAIISLDAMLHLAILSVLYELNIEIPKDVMT
                     ATFNDSYLTEIASPPQTSIDIKPRMLGQQAGSAILNILKNKAQDVIELVIIDTELKIR
                     KSTQR"
     gene            16617..18266
                     /locus_tag="POB55_000862"
     CDS             16617..18266
                     /locus_tag="POB55_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDI6499837.1"
                     /translation="MNKQWWKEAVAYQVYPRSFNDSNHDGIGDLPGMIDKLDYLKDLG
                     IDVIWLSPMFKSPNDDNGYDISDYQEIMDEFGTMEDFDCLLKGVHDRGMKLILDLVVN
                     HTSDEHPWFIESKSSKDNPKRDWYIWQDPKPDGSEPNNWESIFNGSTWEYDANTEQYY
                     FHLFSKKQPDLNWGNPEVRDAVFEMMNWWFDKGIDGFRVDAITHIKKTFEAGDLPVPE
                     GKTYAPAFDVDMNQPGIQTWLQEMKDRSLSKYDIMTVGEANGVSPDDADDWVGEENGK
                     FNMIFQFEHLGLWNSGDSHFDVNSYKSVLNRWQKQLENKGWNALFIENHDQPRRVSTW
                     GDDDKYWYESATSHAAVYFLQQGTPFIYQGQEIGMTNYPFESIETFNDVAVKNDYQIV
                     KAQGGDVDALLAKYKDENRDNSRTPMQWDDTLNGGFTNGEPWFPVNPNYKTINVAQQL
                     EDEHSVLQFYKDLIQLRKSNDVYVYGQFDLVDAENSQVFAYTRTLNEKQVLIVGNLTN
                     HEAELTVPFDLSHGEVKLFNYDAKVNLKQLRPYEACVIELN"
     gene            complement(18348..19214)
                     /locus_tag="POB55_000863"
     CDS             complement(18348..19214)
                     /locus_tag="POB55_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDI6499838.1"
                     /translation="MDVIKQIQQAIVYIEDRLLEPFNLQELSDYVGLSPYHLDQSFKM
                     IVGLSPEAYARARKMTLAANDVINGATRLVDIAKKYHYANSNDFANDFSDFHGVSPIQ
                     ASTKKDELQIQERLYIKLSTTERTPYPYRLEETDDISLVGYARFIDTKYLSHPFNVPD
                     FLEDLLIDGKIKELRRYNDVSPFELFVISCPLENGLEIFVGVPSERYPAHLESRFLPG
                     KHCAKFNLQGEIDYATNEAWYYIESSLQLTLPYERNDLYVEVYPLDISFNDPFTKIQL
                     WIPVKQSPYDED"
     gene            complement(19444..20928)
                     /locus_tag="POB55_000864"
     CDS             complement(19444..20928)
                     /locus_tag="POB55_000864"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HDI6499839.1"
                     /translation="MSTKNKHIPCLITIFGATGDLSHRKLFPSIFHLYQQDNLDEHIA
                     IIGIGRRDITNDDFRNQVKSSIQKHVKDTNKIDAFMEHVFYHRHDVSNEESYQELLDF
                     SNELDSQFELKGNRLFYLAMAPQFFGVISDYLKSSGLTDTKGFKRLVIEKPFGSDLKS
                     AEALNNQIRKSFKEEEIYRIDHYLGKDMVQNIEVLRFANAMFEPLWNNKYISNIQVTS
                     SEILGVEDRGGYYESSGALKDMVQNHMLQMVALLAMEAPISLNSEDIRAEKVKVLKSL
                     RHFQSEDVKKNFVRGQYGEGYIDGKQVKAYRDEDRVADDSNTPTFVSGKLTIDNFRWA
                     GVPFYIRTGKRMKSKTIQVVVEFKEVPMNLYYETDKLLDSNLLVINIQPNEGVSLHLN
                     AKKNTQGIETEPVQLSYSMSAQDKMNTVDAYENLLFDCLKGDATNFTHWEELKSTWKF
                     VDAIQDEWNMVDPEFPNYESGTNGPLESDLLLARDGNHWWDDIQ"
BASE COUNT         7667 a         2958 c         3944 g         6577 t
ORIGIN      
        1 tatttaagtg tttcagcttt aacgaaatat attaaatata aatttgatca agatccacat
       61 ctccaatctg tattgattaa aggtgagctt tcaaatttca aaaagcattc aagtggtcat
      121 ttatacttta atgtgaaaga taaagaaagc gttataagtg ccatgatgtt caaaggtagt
      181 gcttctaaat taaacttcga acctaaagaa ggagatgaag tcttattaga agcacgtgtt
      241 tctgtttttg aacgtcgtgg aaactatcaa atttatgtaa ataaaatgca attagatggt
      301 atagggaact tatatcaaaa attagaagca ttaaagaaaa aattaactga agaaggttgt
      361 tttgataaag cgaataagaa atcaatacct aagtttccta aaaaaattgc agttttaaca
      421 gcgagtacag gtgccgcaat tcgagatatc cattcaacga taaatagtcg ttttccatta
      481 gctgaacaaa tacaaattag tactttggtt caaggtgaaa aagcaaagga cgacattatt
      541 gaaaaaattg aatatgcaga tagtttaggt gtagatacca ttattgtagg tcgaggcggt
      601 ggatccatag aagatttatg gaattttaac gaagaagctg tcgtccgtgc aatatataat
      661 tgtaagacac cgattatatc agcagtaggt catgaaacag actttacatt aagtgatttt
      721 gctgcagaca tcagagctgc gactccaact caagctgctg ttattgcaac accagaccaa
      781 tatgaattgc tgcaacaaat acagcaatat cagtttacat tgactcgttt catcaagaaa
      841 catttagaac aacaacgtaa acatgttgaa catttgtcat cgtattacaa gtttaaacaa
      901 ccaactttat tgtatgatca acagatacaa cgtcgagatg atttagaaaa gagactgaaa
      961 caacaaattc aggcaacttt tgaacaacag agacatcgat taatgctgtt gcaacaacgt
     1021 tataatttaa aagcattact aagtagtgtt aatcaagaac aacaaaataa tcttcaattg
     1081 acaaaccaac ttgttaaatt attgaattct aagatattaa gttataaaaa tgatttgaaa
     1141 aataaagtag aaaacttaaa caatttaagt ccaactaata caatgttgcg tggatatgca
     1201 attgttaata aaaaagacga agtcattacg agtactaaag atttaacaga aaatgatcaa
     1261 ttgacgttaa caatgaaaga tggcttagta gatgcaaaag ttacgaaagt aaggtgtaat
     1321 aatgactaaa gaaacgcaaa gttttgaaga aatgatgcaa gaattagagc aaattgttca
     1381 aaaattagat aatgaaacag tatctttaga ggaatcatta gatttatatc aacgtggtat
     1441 gaaactatca gcagcttgtg acacaacttt aaaaaatgcc gaaaaaaagg tgaatgactt
     1501 aataaaagaa gaagctgagg atgtaaaaaa tgacgaatct accgatgaat aaattaatag
     1561 atgaagtcaa taatgaatta tcggttgcga taaataaatc agtaatggat actcagctag
     1621 aagaaagtat gttgtattca ttaaatgctg gaggtaaacg catccgacca gttctgttat
     1681 tactcacttt agattcacta aataccgagt atgagttagg tatgaagagc gcaattgcac
     1741 tagaaatgat tcatacatat tcacttattc atgatgacct accagcgatg gataatgatg
     1801 attatcgacg aggaaaatta acaaatcata aagtatatgg tgagtggact gcgatattag
     1861 caggtgatgc tttattaact aaagcatttg aacttatttc aagtgatgat agattaactg
     1921 atgaagtaaa aataaaagtt ctacaacggc tgtcaatagc aagtggtcat gttggaatgg
     1981 tcggcggtca aatgttagat atgcaaagcg aaggccaacc aattgatctt gaaactttgg
     2041 aaatgataca caaaacaaaa acaggagcat tattaacttt tgcggttatg agtgcagcag
     2101 atatcgctaa tgtcgatgat gcaactaaag aacatttaga aagttatagt tatcatttag
     2161 gtatgatgtt ccagattaaa gatgatttat tagactgcta tggtgatgaa gcaaagttag
     2221 gtaaaaaagt gggcagcgat cttgaaaata ataaaagtac gtacgtgagt ttattaggga
     2281 aagatggcgc agaagataaa ttgacttatc atagagacgc agcagtggat gaactaacgc
     2341 aaattgatga acaattcaat acaaaacact tattagaaat cgttgattta ttttatagca
     2401 gggatcacta aaagcaaata tatcgattag caacaattgt aaagtttgaa atgtctcaaa
     2461 aggcatattc aactgacgtt gtttgttaat cgatttttct catgttgaaa cttaaaatta
     2521 tgttgaagac tttcatttaa aaaatagaat tttcaatttc tatacgacaa tattcgtatc
     2581 tgtgatatca atgtgatttt aattaaatat gttcaaacaa ttgacgctta ttaaatctaa
     2641 taattagcaa gtttattaca catatattca tttttataca tattttaagc aattgttatg
     2701 cgtaatatga aaaatattta taaatagata accaagatag ataaaccatg tttacccttt
     2761 ttacgtaaag gtgtttagta gtcgtaaatc agtgcatgtg ttaaaataaa tgtataaata
     2821 ttcgtaatca gaggtgttta ctgtgcccaa aaaatcggtt aggcatataa aaattagaga
     2881 aattatttca aatgaacaga tagagacaca agatgaatta gttaaacgat taaacgatta
     2941 tgatttaaat gtcactcaag caactgtttc tcgtgatatt aaagaactac aacttattaa
     3001 agtacctata ccttcaggtc aatatgttta tagtttacca aatgatagaa aattccatcc
     3061 tttagaaaaa ttgggacgtt atttaatgga ttcctttgtt aatatagatg gtactgataa
     3121 tttacttgtt ctaaaaacgt tacctggtaa tgcacaatct attggagcta tattagacca
     3181 aatcaattgg gaagaagtac taggcacaat ttgtggtgat gatacttgtt taattatttg
     3241 tcgaagcaaa gaggcaagtg atgaaatcaa gtcaagaatt ttcaatttgt tataaggatg
     3301 cgatgagttt atgttacaaa ccttatcaat caagcaattt gctattattg aagagttaga
     3361 aattcaattt tctgatggtt taactgtttt aagtggtgaa acgggttcag gtaagtcaat
     3421 tattatagat gcaattggac aattaattgg aatgagagct tcatctgatt ttgtaagaca
     3481 tggtgaaaaa aaggctgtca tcgaaggtat atttgatatt gacgagagta aagatgctat
     3541 acatatattg aaaaatatgg atattgatgt agatgaagat tttttattag ttaaacggga
     3601 aattttcagc tctggtaaaa gtctttgtaa aattaataat caaacagtta ctttacaaga
     3661 tttacgaaaa gttatgcaag aattattaga tattcatggc caacatgaaa cgcagtcttt
     3721 gttgaaacaa aaataccatt taacattatt ggataattac gcagaatcac gttatcaaga
     3781 tttgttggat aaatatcatc aaacttttca aaattataaa gccaaaaagc aagagttaga
     3841 agatttagaa tcagcagacc aagcattgct gcagcgttta gatttaatga aattccaatt
     3901 agaagaattg tctgaagcac atctaaaaga aggcgaaata gagcaacttg aaatagatat
     3961 taaacgcatt caaaactctg aaaaattaag cttagcgctt aataacgcac atatgacatt
     4021 aacggatgaa aatgctatca ctgatcgttt gtatgaacta agcaaccatt tgttaacaat
     4081 aaacgatata gttccaaaca aatatgacaa attaaaagaa gatattgatc aattttacta
     4141 cattttagaa gatgcaaaac atgagttata tgatgaaatg gctaatacag aattcgacga
     4201 acaagtgtta aacgagtatg aatctcgtat gaatttgctg aataatttaa aacgtaaata
     4261 tggaaaagat atttcagaat taatcgcata tcaagaaaag cttaataatg aaatcaataa
     4321 aattgaaaac tatgaacaaa gtacatcgca gttacgagaa gaaataaatg cattgtataa
     4381 tcaagttata gaggttggac aagcgttatc aaagcagcgt cgtatagtcg ctagggagtt
     4441 aagagaccac attgtatcgg aaattcaaaa cttacaaatg aaagacgcaa atcttgagat
     4501 ttcatttaaa aaattagaag aaccgaatat tgatggaatc gaatttgtag aatttttaat
     4561 cagtccaaat aaaggggaac cattaaaaag tttaaataaa attgcgtcag gtggagaact
     4621 ttctagaatt atgttagcgt taaaaagtat ttttgttaaa tcgagaggtc aaactgcaat
     4681 tttattcgat gaggttgact caggtgtatc tggacaagct gcacaaaaaa tggcagagaa
     4741 aatgcgagat atagctgaat atatacaagt tatttgtatc tctcacttac cacaagttgc
     4801 atcgatgagc gaccatcatt tattaatttc gaaatcatca aaagatgatc gtacaacaac
     4861 acaagttcaa gagttaatta gcgatgataa agtagatgaa attgcacgaa tgatttcagg
     4921 tgcaagtgtt actgatttaa ctcgagaaaa tgcaagagaa atgatacaac acaatcaaag
     4981 acgtagataa tttccaaagt gtgttgaata ctcatttatg gatttattta tgtgcattca
     5041 aactaaatag acaatcatgt aaaataggta attaatggac atgaaaacta tgtattaata
     5101 aattttcaat ggatatatat cattagttag gagtaagaac atgtcagaga aacaatatga
     5161 tttagtcgtt ctcggtggag gtactgcagg atatgttgct gcgatacgtg cgtctcaatt
     5221 aggtaaaaaa gtggcaatag tagaacgaca actattagga ggtacttgtc tccataaagg
     5281 ttgtattcct actaaatcgc tgcttaaatc tgctgaagta tttcaaacag taaaacaggc
     5341 agcaatgttt ggcgttgatg tcaaagatgc taatgttaat tttgaaaata tgttggcacg
     5401 aaaagaagac attattaatc aaatgtatca aggtgtaaag catttaatgc aacacaatca
     5461 cattgacatt tataatggca cgggacgtat tctaggtaca tctatatttt cacctcaaag
     5521 tggcacaatt tctgtcgaat atgaagatgg tgaatcagac ttattaccaa accaatttgt
     5581 attaattgca actggttctt cgcctgcaga gttgccattc ttatcatttg atcatgataa
     5641 aattttatct agcgatgata tattatcatt aaagacgtta ccatcaagta ttggtattat
     5701 tggtggtggt gttatcggaa tggaatttgc atcattgatg atagatttag gtgttgacgt
     5761 aacagttata gaagcaggtg aaagaatttt accaactgaa agtaaacaag cttcacaact
     5821 attaaaaaaa tcattgtcag cacgtggtgt taaattctat gagggaataa aactttctga
     5881 aaatgatata aatgttaacg aagatggtgt tacgtttgaa atttcctcag acataattaa
     5941 agtagataaa gtgttgctat cgattggtag aaaacctaac acatcagata taggtttaaa
     6001 caacactaaa ataaaacttt caacatcagg acatatttta acgaacgaat ttcaacaaac
     6061 tgaagataaa catatttatg cagctggtga ttgtattgga aaattacaat tggcacatgt
     6121 tggatcaaaa gaaggtgttg tggctgttga tcatatgttt gaggggaacc caatcccagt
     6181 aaactataac atgatgccta agtgtattta ttcacaacct gaaattgctt ctatcggttt
     6241 aaacattgaa caagcaaagg cagagggaat gaaagttaaa agttttaaag taccatttaa
     6301 agcaattggt aaagcagtga ttgatagcca tgacacaaac gaagggtata gcgaaatggt
     6361 gattgatcaa tcaactgaag aaattgtggg tattaatatg attggtccac atgtaacaga
     6421 attgattaat gaggcatcac tgttgcagtt catgaatggc tcggcattag aattaggact
     6481 aacaacacac gcacatcctt ccatctctga agtgttgatg gaattaggat tgaaagcaga
     6541 aagtagagct attcacgtat aaataggagg atttaacatg attgattata aatcattagg
     6601 ccttagcgaa gaagacctaa aagtaatata taaatggatg gatttaggta gaaaaataga
     6661 tgaaagactg tggttattga accgtgcagg taaaattcca tttgttgtaa gtggtcaagg
     6721 acaagaagca actcagattg ggatggcata cgcattagaa gaaggtgata ttacagcgcc
     6781 ttattataga gatctagctt tcgttactta tatgggtatc tcagcgtatg atacgttttt
     6841 atcagcgttt ggaaagaaag acgatgttaa ctctggtgga aaacaaatgc catcacactt
     6901 tagtagtaga gctaagaata ttttgtctca aagttcaccg gtagcaactc aaatacccca
     6961 tgctgttggt gctgctttag ctttaaaaat ggatggtaag aagaaaattg caacagcaac
     7021 tgttggtgaa ggtagctcaa accaaggtga cttccatgaa ggtttaaact tcgcgggtgt
     7081 acacaaactt ccttttgtat gtgtcattat aaataataaa tatgcaattt cagtacctga
     7141 ttcattacaa tacgcagctg aaaaattatc ggatagagca cttggttacg gtatacatgg
     7201 tgaacaagta gatggtaatg atccattagc aatgtataaa gcgatgaaag aagcaagaga
     7261 tcgagctatt tctggtcaag gttcaacttt gattgaagca gtaacaagtc gtatgacagc
     7321 tcattcatca gatgatgacg accaatatcg tacgaaagaa gagcgtgaag cgcttaaaaa
     7381 agctgactgc aatgaaaagt tcaaaaaaga gttgctttca gctggcatta tcgatgatgc
     7441 ttggttggca gaaatagaag cggagcataa agacatcatc aataaagcga caaaggctgc
     7501 tgaagacgcg ccatatccaa gtgtcgaaga agcctatgca tttgtgtacg aagaagggag
     7561 tcttaactaa tggctaaatt atcttattta gaggcgatac gccaggcaca agacttagca
     7621 ttacaacaaa ataaagatgt gtttattctt ggtgaagatg ttggtaaaaa aggtggcgta
     7681 tttggtacta ctcaaggact acaacaacaa tatggtgaag acagagttat cgatacgcca
     7741 ttagctgagt caaatattgt tggaactgcc attggtgcag ctatggtagg taaaagacca
     7801 attgcagaaa ttcaatttgc agattttatt ttacctgcga caaatcaaat tattagtgaa
     7861 gctgcgaaaa tgcgttatcg ttcaaataat gattggcaat gcccattaac gattcgtgca
     7921 ccatttggtg gcggtgttca cggaggacta tatcattcac aaagtattga aagtatattt
     7981 gcttcatcac caggtttaac aattgttatt ccttccacac cgtatgatgc taaagggctt
     8041 ttattatcat ctatcgaatc aaatgatcca gtattgtact ttgagcataa aaaagcttat
     8101 cgtttcttaa aggaagaagt gccagaagaa tattacactg tacctttagg aaaagcagat
     8161 gttaaacgtg aaggtgaaga tttaacagta ttttgttatg gattaatggt caattattgt
     8221 ttacaagcag cggatatttt agctgcagac ggtatcaacg ttgaagtcgt tgatttacga
     8281 actgtttatc cattagataa agaaacaatt attgatcgtg ctaaaaatac aggtaaagtt
     8341 ttacttgtaa ctgaagataa tttagaaggt agtattatgt cagaagtgtc agcgattatt
     8401 gcagagcatt gcttgttcga tttagatgca ccaatcatgc gtttagctgc tccagatgta
     8461 ccatctatgc cattttctcc tgtattagaa aatgaaatta tgatgaatcc agaaaaaatc
     8521 ttaaataaaa tgcgtgaatt agcagaattc tagggaggga aagtcatgga aataacaatg
     8581 cctaagttag gtgagagtgt tcatgaaggt accattgaac aatggttagt ttctgttggt
     8641 gatcatattg atgaatatga accattatgt gaagttatta cagataaagt gacagctgaa
     8701 gtcccttcca cgatatcagg aacaattaca gaaattttag ttgaagcggg gcagacagta
     8761 gctattgata caattatctg taaaattgaa actgctgatg aaaagacaaa tgaaacaact
     8821 gaagagatac aagcaaaagt ggatgagcat actcagaaat ctactaaaaa agctagtgca
     8881 acagtggaac agacatttac tgctaaacaa aatcaacctc gtaataatgg tcgcttttca
     8941 cctgttgtat ttaaactcgc ttcagagcat gacattgatt tatcacaagt tgtaggtagt
     9001 ggatttgaag gtcgtgtaac taagaaggat ataatgtcag ttattgaaaa tggtggtacc
     9061 acagctcaat ctgacaaaca agttcaaaca aaatcaacat cagtagatac atcaagtaac
     9121 caatcatctg aagacaatag tgaaaacagc acaataccag taaatggtgt gcgtaaagca
     9181 attgcgcaaa atatggttaa tagtgtaaca gagattccac atgcatggat gatgattgaa
     9241 gtagatgcta caaatcttgt gaatacgaga aatcattata aaaacagctt taaaaataaa
     9301 gaaggatata atctaacgtt ctttgctttc tttgtaaaag ctgtagcaga tgctttaaaa
     9361 gcatatcctt tattaaatag tagctggcaa ggaaatgaaa ttgtcttaca taaagacatt
     9421 aatatttcaa ttgctgttgc tgatgaaaat aaattatacg tacctgtgat taagcatgca
     9481 gacgaaaagt caatcaaagg tatagctaga gaaattaata ctttagcaac gaaagcgcgt
     9541 aataagcaat tgacagctga agatatgcag ggcggtacat ttacggtaaa taatactggt
     9601 acatttggtt cagtatcatc aatgggtatt ataaatcatc cacaagcagc gattttacaa
     9661 gtagaatcaa tcgttaaaaa gccagtagta attaatgata tgattgcaat tcgtaacatg
     9721 gttaatttat gtatttcaat tgatcatcgt attttagatg gtttacaaac aggtaaattt
     9781 atgaatcata ttaaacagcg tatcgaacag tatactttag aaaatacaaa tatatattag
     9841 tgataacata gatgcatcta tcgacaactt gttttatctt gttcttgtcg atggatgtat
     9901 tattttttgg cactaaaata tgtgcaatat attcaaaaaa gataaagaac aataatcaac
     9961 atggttgaat gcatttttgc agtaaagtca aaataagaca tcatacttga aacatattaa
    10021 tgaaaacatg tgaacaaatt agttaccatg attttaagca caataatgtt tggtatattg
    10081 ttaaaattgt gtctaaatat aggtgtgatt cagattagtt tattgaacaa tatgttatta
    10141 attagtagaa tgaggatagt ttaaatataa agggataggt gattgaactt atggacatga
    10201 atttcgattt atacatgaac ggtgttgtag aacaagcaag gaatgaaatt gaatctgcgg
    10261 gatatgagca attaactact gcagaagatg ttgacaaagt tcttaaacaa gatggtacaa
    10321 cactagttat gatcaattct gtatgtggtt gtgcaggtgg tatcgcaaga ccagcagcat
    10381 cacatgcttt acattatgac gtattacctg atcgtctagt gacagtattt gctggacaag
    10441 ataaagaagc gacacaaaga gcgcgtgaat acttcgaagg ttatgcgcct tcaagtccgt
    10501 catttgcatt agtaaaagat ggaaagatta cagaaatgat tgaaagacat caaatcgaag
    10561 gtcatgatgt gatgaacgta attaatcaat tacaaacatt attcaataaa tattgtgaag
    10621 aaagataaga ggcgctaacc catgttaaag ttaaatcctt acaagattgg atttagaaca
    10681 ataaaaacag cagtgggtat gactttaggt gtaattatta gtaagctgtt aggtttagat
    10741 aattatgctt caagcgccat attagtcgta ttatgtatta aacatacaaa agtacattcg
    10801 ctacaagcga ttatttcaag attagtatca tgttttttag tattgttttt aggttcagca
    10861 atatttagtt tattaggtca gagtccaatt gtactcggta ttatcgtatt gttatttata
    10921 ccattaactg tcgtattaaa agtacaagaa ggtgtcatta cgagttgcgt tatattactt
    10981 catgttttta atgcaaaatc aattgatgca catttaattg ttaatgaaac attattactg
    11041 ttaattggac tgagcattgc atttacaatg aatttaatga tgccaagttt agacaaacaa
    11101 ctagacgaat acaaatgtaa aattgagcaa caaattgctg atatttttag taaatatagt
    11161 tatatttgtg aaaagtatga agataccatt gcgattgaat ttgaagtgtt acttttaaat
    11221 attaaaaagg cgaagtctat cgcgttccga gatgttaaaa atcattttgt tagaaacgaa
    11281 aattcatact atcattattt tgatatgcga gaagagcaag tggaattgtt aatgagaatg
    11341 aaaccgctca tcgaaagtat ctgtcataaa gatccacttt taaatgatgt tgcgtcactg
    11401 ctgaaagaaa ttggtctaaa tgtgaatagt aatgactata cagcaatgcg tttacacaat
    11461 ttgtatgaga cgcgtttgaa attggatgaa cttccattgc caacaacaca caaagcgctt
    11521 aactcgagag caagtattat tcaaatatta aatgaattag aagaatattt acaaatcaaa
    11581 tcgcaatttg gttcattgaa aatgcatagt gaagtagctt aaaaaataat ccatccgtat
    11641 gacccatgtt tgaaaataat cgttgcaata cgataactta ttaatacatg cgtttataac
    11701 ggatggattt ttaattgtat gattttgtat aggtagttga gatatagttt ataaaattgg
    11761 aatgatactc gttaataaca atgctaatac aactgataca agcataacga taatgataat
    11821 tcttaaaact ttattttgca ttttgtgtgc ctccaaatga ataacctttg tattaataat
    11881 tacgacataa gatataaaac ttttaacttg agacgatata actagcaatc atcattaagg
    11941 aatataataa aaaacatgag taagtttgct tatgtcattt tcctcattat acgatatctt
    12001 tgaaaaatca atcaatatat ttttgacaaa gcttagtttg agttacaata aatatatata
    12061 ataaattgaa aataatatga cacaatttac aagcctgata aatctgtgta acatcaatta
    12121 ctgtaacatt ttatttcatc aatttttacc gtttggcgta catgaaaatt ttgataaaag
    12181 gggttccatt atgattaatg agcaaagatt acttaatacg ttttttgaac ttgtacaaat
    12241 cgattctgag acagggaatg aatcaacaat ccaacctatt ttaaaagaaa aattcatagc
    12301 attagggtta gatgttaaag aagatgaagc ggctaaacat cctaaattag gtgccaataa
    12361 tctagtgtgt acaatgaata gtactatcga agaaggcgaa gtacctaaat tatatttgac
    12421 gagccatatg gatactgttg tacccgcaat taatgtaaag ccaattgtaa aagatgacgg
    12481 ctacatatac tctgatggta cgactatact aggtgcagat gataaagcag gattagcagc
    12541 gatgcttgaa gttttgcaag tgataaagga acaacaaatc ccacatggac aaattcaatt
    12601 tgtgattact gttggggaag aatcaggttt aattggtgct aaagaattaa attcagagtt
    12661 gttggacgcg gactttggtt atgctattga tgcaagtgct gatgtcggca ctacggttgt
    12721 aggtgcaccg acgcaaatgt taatttcagc taaaattttt ggcaaaacgg ctcatgcaag
    12781 tacgccaaaa gagggtgtta gtgctattaa tattgcggcg aaagcaatta gtcgaatgaa
    12841 attaggacaa gttgatgaaa taactactgc caatattggt aagtttcatg gcggttcagc
    12901 tacgaatatt gttgcagatg aagtcatttt agaagcagag gcacgttctc acgatccaga
    12961 aagaataaaa acacaagtca aacatatgac tgatgtgttt gaaactactg catcagaatt
    13021 aggtgggaaa gcagaagtta cagtggagca aagttatcca ggatttaaaa ttaatgataa
    13081 tgaagcggta gttaaaattg cacaagaaag tgctcgtaat ttaggattat ctgcaaatac
    13141 aattatttct ggtggcggtt cagacggcag tattattaat acatttggta ttccttcagt
    13201 cattttagga gtgggttatg aaaaaatcca tacaacaaat gagagaatgc cgattaaatc
    13261 attgaacttg cttgcaagtc aagtgttaga aataatcaaa atagttgcgc gacaatcgaa
    13321 atgatttagt gtagtgtatg tctattttgt gatacaatac taatgaaaaa tcaacaagtg
    13381 aggtaagtaa tatgacacaa caaattggag ttattggttt agccgttatg ggtaaaaacc
    13441 tagcttggaa tattgaatca cgcggatata gtgtatctgt atttaaccgc tcaagtgaaa
    13501 aaactgattt aatggttgaa gaatcaaaag ggaaaaatat tcatccaacg tactcattgg
    13561 aagaatttgt taattcttta gaaaaaccac gtaaaatttt attaatggtt caagcaggta
    13621 aagctacgga tgcaacgatt gatagtttgt tacctttatt agatgatgga gatattttaa
    13681 ttgatggtgg taatacaaac tatcaagata ctatcagacg taataaagca ttagcacaaa
    13741 gtgcaatcaa ctttattggt atgggcgttt ctggtggtga aattggagcg ttaactggtc
    13801 cgtcattaat gccgggtgga caagaagaag cttataacaa agttgcagat attcttgatg
    13861 caattgctgc aaaggcaaaa gacggtgctt catgtgttac ttatattgga ccaaatggtg
    13921 ctggacacta tgtgaaaatg gtacataatg gtattgaata tgctgacatg caattaattg
    13981 cagaaagtta tgctatgatg aaagaattat taggcatgtc tcatgaagac attgctcaaa
    14041 cgttcaaaga ctggaatgct ggtgaattag aaagttattt aattgaaatc actggtgata
    14101 ttttcatgaa attagatgaa aataaagaag cattagttga aaaaattctt gatactgcag
    14161 gtcaaaaagg tacaggaaaa tggacttcaa ttaacgcatt agaattaggt attccattaa
    14221 caatcattac tgaatctgta tttgcacgtt ttatctcttc aatcaaagaa gagcgcgtaa
    14281 atgcttctaa agaattaaat ggtccaaaag catcatttga tggtgacaag aaagactttt
    14341 tagaaaaaat tcgtaaagca ttatatatga gtaaaatttg ttcttatgca caaggtttcg
    14401 cgcaaatgcg taaagcaagt gaagataatg aatggaattt aaaacttggt gatttagcta
    14461 tgatttggag agaaggttgc attatccgtg cacaattctt acaaaagatc aaagatgctt
    14521 acgataataa tccaggatta caaaacttat tgctagatcc ttactttaaa aacatcgtta
    14581 ctgaatatca agatgcgtta cgtgatgttg tcgcaactgg tgttcaaaat ggtgtaccaa
    14641 cacctggttt ctcatcaagt attaattact atgatagtta ccgtgcagct gatttacctg
    14701 caaacttaat tcaagcacaa cgtgactact tcggtgctca tacgtacgaa agaaaagata
    14761 aagaaggcgt attccataca caatggattg aagaataata ttgattaaag gatagttgaa
    14821 agatactatc cttttttata tcttttgata aatataaaag agagtgtgat agagtagcta
    14881 caacctataa aaaattacac aagcaatatc tattacctta aaagcatccc gccatatttt
    14941 taagcatttt gtgaagtaat aaaatattta ctgtgttatg atagcatgta ctatagagaa
    15001 atagaggtat taacttatgg aaaagttaaa tcaagttatg ttatatgttg atgatgttga
    15061 caaagcgaaa gcattttgga cagaaacttt agaatttgtt gttgtaagtg aaacaccgtt
    15121 agctgaggac tatgttgcag ttgaagtatc acctacgaaa gatgctgaaa cgtcattgac
    15181 gattatggca aaagaattta ttgaaaagta tagcccggaa gtgaacctag gaacaccatc
    15241 tttaatgttt aaagagaaaa actttgatgc attatactca aaattaaatg atttaggttt
    15301 aactgggcat gatatagtag aaatgaatgg tcaacgtgta tttaattttc aagatggcca
    15361 aggtaactat tttgcagtta gtgactaaac aatcgatttt aatatttata caaacctata
    15421 tattgatttt cggattgaat ttcagtatga aggttttatt tttatcgttt tatgcaatcg
    15481 tttgcacaaa tcttttgact tttttagaaa gtgtgactaa actggtatta tacagtggtt
    15541 ggaagcgttt tccgatgcag aaattaacag gaggtgatta aatggttacg attaaagatg
    15601 ttgcactaaa agccggtgtt tctccttcga cagtttcaag agttataaaa ggaaatcaac
    15661 gtattagcga agcgacaatt tcaaaagtga agaaagttat ggaagaattg aattattttc
    15721 ctaataccgc tgctagaact ttaattacaa accaaacata taaaattggt ttagtgttaa
    15781 aagggtctga ggagcctatt cgactgaatc cattctacat caatgtattg ctaggaattt
    15841 ctgaaacgtg taaccagcat ggctatggta cacaaacgac agtctcaaat aatatgaatg
    15901 atttaatgga tgaagtttat aaaatgatta aacaacgaat ggttgatgcg tttatactgc
    15961 tttattcaaa agaaaatgat ccaattaaac aaatgttaat tgatgaaagc atgccattta
    16021 ttgtgattgg taagcctaca tcggatatag atcatcaatt tacacacata gataatgata
    16081 atatattagc ttctgaaaat ttgacacgac atgttattga acaaggtgta gatgaattaa
    16141 tatttattac agaaaaagga aattttgaag tttcaaaaga tagaattcaa ggatttgaaa
    16201 cggttgcatc acaatttaat ctggattatc aaattattga gactagtaat gaaagaaaag
    16261 ttatttttaa ttacatgcaa aatctacata cgcgcttgaa ggacccaaat attaaacagg
    16321 caatcatttc gttagatgct atgttacatt tagcgatttt aagtgtgcta tatgaactta
    16381 atattgaaat tccaaaagat gtaatgacag caacattcaa tgattcttat ttaaccgaaa
    16441 ttgcgtcacc acctcaaacc agtattgaca tcaagcctcg aatgttaggt caacaggctg
    16501 gttcagcaat tttaaatata ttgaaaaaca aagcacagga tgttattgaa ctcgtcatta
    16561 tagatacaga attaaaaata agaaaatcaa cacagcgata gcaaaggagt tttaaaatga
    16621 ataagcaatg gtggaaagaa gcagtagcat atcaagtata tccaagaagt tttaatgata
    16681 gtaatcacga tggtattggg gatttacctg gaatgattga taaattggac tacttaaaag
    16741 atttaggtat cgatgtcatt tggctcagtc caatgtttaa atcacctaat gatgacaatg
    16801 gttatgatat tagtgactat caagaaatta tggatgaatt tggaacgatg gaagactttg
    16861 attgtttatt aaaaggtgtt catgatagag gcatgaagct tattttagat ttagttgtaa
    16921 atcatacatc tgatgaacat ccttggttta tagaatccaa atctagtaaa gacaatccca
    16981 aacgtgattg gtacatttgg caagatccaa agccagatgg ctctgaacct aacaactggg
    17041 aaagtatatt taatggatct acatgggaat atgatgctaa tactgagcaa tattatttcc
    17101 atttattcag taaaaaacaa cctgatttga attggggtaa tccggaagtt agagatgctg
    17161 tatttgaaat gatgaactgg tggtttgata aaggcattga tggatttaga gtagatgcaa
    17221 ttacgcatat taagaagacg tttgaagcgg gtgacttacc tgtacctgag ggtaaaacat
    17281 atgccccagc atttgatgta gatatgaatc agccaggtat acaaacttgg ttacaagaga
    17341 tgaaagatcg ctcattaagt aagtatgaca ttatgactgt tggtgaagcg aatggtgtaa
    17401 gccctgatga tgctgatgac tgggtcgggg aagaaaatgg taaatttaat atgatattcc
    17461 aatttgaaca tttgggactg tggaatagtg gtgattctca ctttgatgta aattcgtata
    17521 aatctgtatt aaatagatgg caaaaacaac ttgaaaataa aggttggaat gcgttgttta
    17581 ttgaaaatca cgaccaacca cgacgtgtat cgacgtgggg tgacgatgac aagtattggt
    17641 atgaatcagc aacaagtcat gcagctgttt atttcttgca acaaggtacg ccattcattt
    17701 atcaaggtca agaaattggt atgacgaatt atccatttga aagtattgaa acgtttaacg
    17761 atgttgctgt taaaaatgac tatcaaatag tgaaagctca aggtggagat gtagacgctt
    17821 tacttgcgaa atataaagat gagaaccgag ataattctcg cacaccaatg caatgggatg
    17881 atacgttaaa tggaggattt acaaatggtg aaccgtggtt cccagtgaat ccgaattata
    17941 aaactatcaa tgttgcacaa caattagaag atgagcattc agtattacaa ttttataaag
    18001 atttaattca attaagaaag tctaatgatg tatacgtata tggtcaattt gatttagtag
    18061 atgctgaaaa ttcacaagtt tttgcgtaca cgagaacatt aaatgaaaag caagttctta
    18121 ttgtaggtaa tcttactaac cacgaagcag aattaactgt accatttgat ttaagccatg
    18181 gagaagtaaa gctatttaat tatgatgcca aagttaattt aaaacagtta cgtccatatg
    18241 aagcatgtgt tatcgaacta aattaacata ttaatatgcc ccctaataga atctaaggcc
    18301 atttgtagac ctatagatat attagggggc ttctttgttt ttaattttta atcttcgtca
    18361 taaggactct gtttaacagg aatccaaagc tgaattttag tgaatgggtc attaaatgaa
    18421 atatcgagag ggtacacttc aacatataaa tcatttcgtt catatggcaa tgttaactgc
    18481 aaacttgatt caatatagta ccaagcttca ttagttgcat aatcaatttc accttgtaaa
    18541 ttgaatttcg cacaatgttt gccaggtaaa aatcggcttt ctaagtgtgc aggataacgt
    18601 tcacttggta cacctacaaa tatttctaaa ccattttcaa gaggacaact aataacaaat
    18661 agttcaaatg gactaacgtc attatatcgt cgtaactctt taattttacc atcaatgagc
    18721 aagtcttcta aaaaatccgg aacattaaaa ggatgtgaca aatacttagt gtctataaat
    18781 cgtgcatatc caaccaatga aatatcatct gtctcttcta atctgtatgg atagggtgtt
    18841 ctctcagtag ttgataattt gatatataat cgctcttgaa tttgtaattc atctttttta
    18901 gtagaagctt gaataggtga tacgccgtga aaatcactaa aatcatttgc aaaatcattt
    18961 gaatttgcat agtgatattt tttagcgata tctacaagtc gtgtagcacc attaatcaca
    19021 tcatttgcag cgagtgtcat tttacgcgcg cgtgcataag cttctggaga taagccgaca
    19081 atcattttaa atgattgatc aagatggtat ggcgaaagac caacgtaatc acttaattct
    19141 tgcaaattga aaggctctaa taaacgatct tcaatataaa caattgcctg ttgtatttgc
    19201 ttgataacgt ccaaaacttt cactccaatt aattcaatca tttattttta ttctacatta
    19261 tttctataaa ttatacaccc atttgttcaa tgattattaa aatagttttg ggcattgtaa
    19321 aatataattt cataatatag tctagaaaaa aagcgaatga tagaacaatt gatttacttg
    19381 attcgtaatc aatccttgtc attcgctcat ttatttttgt ttaacatgtg cgttttaatt
    19441 caattattga atatcgtccc accaatggtt accatcacga gcaagtagta aatcactttc
    19501 taatggacca ttagtacctg attcatagtt agggaattct ggatcaacca tattccattc
    19561 atcttgaatt gcatcaacaa atttccatgt tgattttaat tcttcccagt gcgtgaagtt
    19621 agtggcatca cctttaagac aatcgaataa tagattttca tatgcatcta cagtattcat
    19681 tttatcttga gcgctcattg aataagacaa ttgaacaggt tcagtttcga taccttgtgt
    19741 atttttctta gcatttagat gtaaagatac accttcatta ggttggatat tgattactaa
    19801 taggtttgaa tctaatagtt tatcagtttc atagtataag ttcattggta cttctttaaa
    19861 ttcaacgaca acttgaattg ttttagattt catacgttta ccagtacgaa tatagaatgg
    19921 tacaccagcc catctaaagt tatcaattgt taatttacct gaaacaaagg taggtgtgtt
    19981 agagtcatct gcaacgcgat cttcatcacg gtatgcttta acttgtttac catcgatata
    20041 gccttcgcca tattgaccac gaacaaagtt ctttttaaca tcttcagatt ggaaatgacg
    20101 cagtgattta agtactttta ctttctcagc acggatatct tcactattta aactaatagg
    20161 tgcttccata gctaataatg caaccatttg taacatgtgg ttttgcacca tatcttttag
    20221 cgcgccactt gattcataat aaccaccacg atcttcaaca cctagtattt cagatgatgt
    20281 aacttggatg tttgaaatat atttgttatt ccataatggt tcaaacatcg cattcgcaaa
    20341 acgtaatacc tcgatatttt gaaccatgtc ttttcctaaa tagtggtcaa tacgataaat
    20401 ttcttcttct ttaaatgatt tacgaatttg attgtttaat gcttcggctg attttaaatc
    20461 actaccgaat ggtttttcga taacaaggcg tttaaatcct tttgtatcag taagaccaga
    20521 agattttaga taatcagaaa taacgccaaa gaattgtggt gccattgcta aatagaatag
    20581 tcgattacct tttaattcaa attggctatc taattcatta ctaaaatcta gtaattcttg
    20641 atagctttct tcattactaa catcatgtct atgatagaag acatgttcca taaacgcgtc
    20701 aattttgttt gtatctttta cgtgcttttg aattgatgat tttacttgat tacggaaatc
    20761 atcattagta atgtcacgac gtccaatacc gatgatggca atatgttcat ctaaattgtc
    20821 ttgttggtag agatggaata ttgatggaaa caacttacga tggcttaagt caccagttgc
    20881 accaaagatt gtgattaaac aagggatgtg tttgttttta gtactcaaga ttaaaacctc
    20941 aattcttaat tagatatatg atttattatt ataagataat ccattcagca ggtcattcaa
    21001 tatgctcgac agtatgcagt gtcttaaatg aaatatcgat tcatgtatca tgtttaatgt
    21061 gatacttatt aatgataagt ataacgtaat tatcaaaatt tatatagttt tgtctacgtt
    21121 aaagttagaa aaattaacta gcaaag
//
LOCUS       DAMZKC010000031        20698 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.031, whole genome shotgun sequence.
ACCESSION   DAMZKC010000031 DAMZKC010000000
VERSION     DAMZKC010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 20698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20698
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.031"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            153..353
                     /locus_tag="POB55_000865"
     CDS             153..353
                     /locus_tag="POB55_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1514 family protein"
                     /protein_id="HDI6499840.1"
                     /translation="MWITMTIVFAILLLVCISINSDRAREIQALRYMNDYLLDEVVKT
                     KGYNGLKEYRIELKRMNNDIKK"
     gene            381..797
                     /locus_tag="POB55_000866"
     CDS             381..797
                     /locus_tag="POB55_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDI6499841.1"
                     /translation="MIKIEKHDIKKLEEYIQHIDNYRRELKMREYELLESHEPDNAGA
                     GKSNLPGNPIERCAIKKFSDNRYNTLRNIVNGVDRLIDESDEDTLELLRFRYWDCPIG
                     CYEWEDIAHYFGTSKTSILRRRNALIDKLAKYIGYV"
     gene            1029..1328
                     /locus_tag="POB55_000867"
     CDS             1029..1328
                     /locus_tag="POB55_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000988331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDI6499842.1"
                     /translation="MMTKDERIRFYKSKEWQTTRKRVLERDNYECQQCKRDGKLTTYD
                     KRKRKSLDVDHILSLEHHPEFAHDLNNLETLCIKCHNKKEKRFIKKENKWKDEKW"
     gene            1458..1802
                     /locus_tag="POB55_000868"
     CDS             1458..1802
                     /locus_tag="POB55_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499843.1"
                     /translation="MEIIVDENLVLKEKERLQVLYKDIPSNKLKVVDGLIIQAARLRV
                     MLDYMWEDIKEKGDYDLFTQSEKAPPYERERPVAKLFNARDAAYQKIIKQLSDLLPEE
                     KEDTETPSDDYL"
     gene            1799..3460
                     /locus_tag="POB55_000869"
     CDS             1799..3460
                     /locus_tag="POB55_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016187779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDI6499844.1"
                     /translation="MISNKYVDEYINLWKQGKIILNKERIDLFNYLQKHIYSRDDVYF
                     DEQKIEDCIKFIEKWYFPTLPFQRFIIANIFLIDKNTDEAFFTEFAIFMGRGGGKNGL
                     ISAISDFLSTPLHGVKEYHISIVANSEDQAKTSFDEIRTVLMDNKRNKTGKTPKAPYE
                     VSKAKIINRATKSVIRYNTSNTKTKDGGREGCVIFDEIHYFFGPEMVNVKRGGLGKKK
                     NRRTFYISTDGFVREGYIDAMKHKIASVLSGKVKNSRLFAFYCKLDDPKEVDDRQTWE
                     KANPMLHKPLSEYAKTLLSTIEEEYNDLPFNRSNKPEFMTKRMNLPEVDLEKVIAPWK
                     EILATNREIPNLDNQMCIGGLDFANIRDFASVGLLFRKNDDYIWLGHSFVRQGFLDDV
                     KLEPPIKEWEKMGLLTIVDDDVIEIEYIVDWFLKAREKYGLEKVIADNYRTDIVRRAF
                     EDAGIKLEVLRNPKAIHGLLAPRIDTMFAKHNVIYGDNPLMRWFTNNVAVKVKPDGNK
                     EYIKKDENRRKTDGFMAFVHALYRADDIVDKDMSKALDALMSIDF"
     gene            3476..4663
                     /locus_tag="POB55_000870"
     CDS             3476..4663
                     /locus_tag="POB55_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDI6499845.1"
                     /translation="MSILEKIFKTRKDITYMLDLDMIEDLSQQAYVKRLAIDSCIEFV
                     ARAVAQSHFKVLEGNRIQKNDVYYKLNIKPNTDLSSDSFWQQVIYKLIYDNEVLIVVS
                     DSKELLIADSFYREEYALYDDIFKDVTVKDYTYQRTFTMQEVIYLKYNNNKVTHFVES
                     LFEDYGKIFGRMIGAQLKNYQIRGILKSASSAYDEKNIEKLQAFTNKLFNTFNKNQLA
                     IAPLIEGFDYEELSNGGKNSNMPFSELSELMRDAIKNVVLMIGIPPGLIYGETADLEK
                     NTLVFEKFCLTPLLKKIQNELNAKLITQSMYLKDTRIEIVGVNKKDPLQYAEAIDKLV
                     SSGSFTRNEVRIMLGEEPSDNPELDEYLITKNYEKANSGENDEKEKDENTLKGGDEDE
                     SGD"
     gene            4647..5384
                     /locus_tag="POB55_000871"
     CDS             4647..5384
                     /locus_tag="POB55_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HDI6499846.1"
                     /translation="MKAEIKGVIVSNEDKWVYEMLGMDSTCPKDVLTQLEFSDEDVDI
                     IINSNGGNLVAGSEIYTHLRAHKGKVNVRITAIAASAASLIAMAGDHIEMSPVARMMI
                     HNPSSIAQGEAKDLNHAAETLEHVGQIMAEAYAVRAGKNKQELVEMMARETWLNADEA
                     IEQGFADSKMFENDNMQIVASNTQVLSKDVLNRVTALVSKTPEVNIDIDAIANKVIEK
                     INMKEKESEIDVADSKVSANGFSRFLF"
     gene            5408..6553
                     /locus_tag="POB55_000872"
     CDS             5408..6553
                     /locus_tag="POB55_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001573791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDI6499847.1"
                     /translation="MTINLSETFANAKNEFINAVNNGEPQERQNELYGDMINQLFEET
                     KLQAKAEAERVSSLPKSAQSLSANQRSFFMDINKNVNYKEEKLLPEETIDRIFEDLTT
                     NHPLLADLGIKNAGLRLKFLKSETSGVAVWGKIYGEIKGQLDAAFSEETAIQNKLTAF
                     VVLPKDLNDFGPAWIERFVRVQIEEAFAVALETAFLKGTGKDQPIGLNRQVQKGVSVT
                     EGAYPEKEEQGTLTFANPRATVNELTQVFKYHSTNEKGKSVAVKGNVTMVVNPSDAFE
                     VQAQYTHLNANGVYVTALPFNLNVIESTVQEAGKVLTYVKGLYDGYLAGGINVQKFKE
                     TLALDDMDLYTAKQFAYGKAKDNKVAAVWKLDLKGHKPALEGTEETL"
     gene            6573..6857
                     /locus_tag="POB55_000873"
     CDS             6573..6857
                     /locus_tag="POB55_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016170801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499848.1"
                     /translation="MVKFKVVRAFKDIEHNQHKYKVGELYPAEGYNNPRVELLTNQIK
                     NKYDKVYIVPLDKLTKQELLELCESLQKKASSSMVKSEIVDLLNGEDNDD"
     gene            6847..7131
                     /locus_tag="POB55_000874"
     CDS             6847..7131
                     /locus_tag="POB55_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009560502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HDI6499849.1"
                     /translation="MTIDDLLVKFKSLEKIDHNSEDEYLKQLLKMSYERIKNQCGVFE
                     LENLIGQELILIRARYAYQDLLEHFNDNYRPEIIDFSLSLMEVSEDEESV"
     gene            7115..7477
                     /locus_tag="POB55_000875"
     CDS             7115..7477
                     /locus_tag="POB55_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor protein"
                     /protein_id="HDI6499850.1"
                     /translation="MKKVFKKPRITTKRLNTRVHFYKYTENNGPEAGEKEEKLLYSCW
                     ASIDGVWLRELEQAISNGTQNDIKLYIRDPQGDYLPSEEHYLEIESRYFKNRLNIKQV
                     SPDLDNKDFIMIRGGYSS"
     gene            7474..7878
                     /locus_tag="POB55_000876"
     CDS             7474..7878
                     /locus_tag="POB55_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDI6499851.1"
                     /translation="MSVKVIGDKALERELEKRFGIKEMVKVQDKALIAGAKVIVEEVK
                     KQLKPSKDTGALINEVSFSKPEWINGKRTITVHWRGSKDRYKIVHLIEYGHVQKGTGK
                     FIKPKAMGGVNRAIRQGQNKYFETLKRELKKL"
     gene            7875..8282
                     /locus_tag="POB55_000877"
     CDS             7875..8282
                     /locus_tag="POB55_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499852.1"
                     /translation="MIDILYKVHEVISQDRIIREHVNINNIKFNKYPNVKDTDVPFIV
                     IDDIDDPIPTTYTDGDECAYSYIVQIDVFVKYNDEYNARIIRNKISNRIQKLLWSELK
                     MGNVSNGKPEYIEEFKTYRSSRVYEGIFYKEEN"
     gene            8283..8927
                     /locus_tag="POB55_000878"
     CDS             8283..8927
                     /locus_tag="POB55_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDI6499853.1"
                     /translation="MAVKHASAPKAYINITGLGFAKLTKEGAELKYSDITKTRGLQKI
                     GVETGGDLKTAYADGGPIESGNTDGEGKISLQMHAFPKEIRKIVFNEEYDEDGVYKET
                     QGKQNNYVAIWFRQERRDGTFRTVLLPKVMFTNPKIDGETAEKDWDFSSEEVEGEALF
                     PLVDNKKSVRKYIFDSANMTNHDGDGEKGEEAFLKKILGEEYTGNVTEGNEETL"
     gene            8969..9193
                     /locus_tag="POB55_000879"
     CDS             8969..9193
                     /locus_tag="POB55_000879"
                     /inference="COORDINATES: protein motif:HMM:NF014427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HDI6499854.1"
                     /translation="MPDSIKTLKVGDTYDLNVVVEPSNQSKLLKYTTDQTNIVSINSD
                     GQVTAEAQGIATVKATVGNMSDTITINVEA"
     gene            9243..9593
                     /locus_tag="POB55_000880"
     CDS             9243..9593
                     /locus_tag="POB55_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499855.1"
                     /translation="MAKLKRNIIQLVEDPKANEIKLQTYLTPHFISFEIVYEAMDLID
                     DIEDENSTMKPREIADRLMDMVVKIYDNQFTVKDLKERMHAPDGMNALREQVIFITQG
                     QQTEETRNFIQNMK"
     gene            9838..14367
                     /locus_tag="POB55_000881"
     CDS             9838..14367
                     /locus_tag="POB55_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001799555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDI6499856.1"
                     /translation="MGERIKGLSIGLDLDAANLNRSFAEIKRNFKTLNSDLKLTGNNF
                     KYTEKSTHSYKQRIKELDGTITGYKKNVDDLAKQYGKVSQEQGENSAEAQKLRQEYNK
                     QANELNFLEKELEKTTTEFEEFKKAQVEAQRMAESGWGKTSKVFESMGPKLTKMGDGL
                     KSIGKGLMIGVTAPVLGIAAASGKAFAEVDKGLDTVTQATGATGGELKKLQNSFKDVY
                     GNFPADAETVGGVLGEVNTRLGFTGKELESATESFLKFSHITGSDGVQAVQLITRAMG
                     DAGIEADEYQSVLDMVAKAAQASGISVDTLADSITKYGAPMRAMGFEMKESIALFSQW
                     EKSGVNTEIAFSGLKKAISNWGKAGKDPREEFKKTLAEIERTPDIASATSLAIEAFGA
                     KAGPDLADAIKGGRFSYQEFLKTIEDSQGTVNQTFKDSESGSERFKVAMNKLKLVGAD
                     VWASIESAFAPVMEELIKKLSVAVDWFSSLSDGSKRSIVIFGGIAAAIGPVVFGLGAF
                     ISTIGNAVTVLAPLLASIVKAGGLISFLSTKVPILGTVFTALTGPIGIVLGVLAGLAV
                     AFTIAYKKSETFRNFVNGAINSVKQTFSNFIQFIQPFIDSVKNVFKQAVSAIVDFAKD
                     IWSQINGFFNENGISIVQALQNICNFIKAIFEFILNFVIKPIMFAIWQVMQFIWPAVK
                     ALIVSTWENIKGVIQGALNIILGFIKFFSSLFTGNWRGVWDGIVMILKGTVQLIWNLI
                     QLWFVGKILGVVRYFGGLLKGLISGIWGVIKGIFTKSLSAIWNATKSIFGFLYNSVKS
                     IFTNMKNWLSSTWNNIKSNTVGKAHSLFTGVRSKFTSLWNATKDIFTKLRNWMSNIWN
                     SIKDNTVGIAGRLWDRVRNIFGSMRDGLKSIISKIKDHIGGMVDAVKRGLNKLIEGLN
                     WVGGKLGMDKIPKLHTGTEHTHTTTRLVKNGKIARDTFATVGDKGRGNGPNGFRNEMI
                     EFPNGKRVITPNTDTTAYLPKGSKVYNGAQTYSMLNGTLPRFSLGTMWKDIKSGASSA
                     FNWTKDKIGKGTKWLGDKVGDVLDFMENPGKLLNYILEAFGIDFNSLTKGMGIAGDIT
                     KAAWSKIKKSATDWIKENLEAMGGGDLVGGILDPDKINYHYGRTAAYTAATGRPFHEG
                     VDFPFVYQEVRTPMGGRLTRMPFMSGGYGNYVKITSGVIDMLFAHLKNFSKSPPSGTM
                     VKPGDVVGLTGNTGFSTGPHLHFEMRRNGRHFDPEPYLRNAKKKGRLSIGGGGATSGS
                     GATYASRVIRQAQSILGGRYKGKWIHDQMMRVAKRESNYQSNAVNNWDINAQRGDPSR
                     GLFQIIGSTFRANAKRGYTNYNNPVHQGISAMQYIVRRYGWGGFKRAGDYAYATGGKV
                     FDGWYNLGEDGHPEWIIPTDPARRNDAMKILHYAAAEVRGKKASKNKRPSQLSDLNGF
                     DDPSLLLKMIEQQQQQIALLLKIAQSNDVIADKDYQPIIDEYAFDKKVNASIEKRERQ
                     ESTKVKFRKGGIAIQ"
     gene            14364..15737
                     /locus_tag="POB55_000882"
     CDS             14364..15737
                     /locus_tag="POB55_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HDI6499857.1"
                     /translation="MIDTIKVNNKTIPWLYVERGFEIPSFNYVLKTENVDGRSGSIYK
                     GRRLESYSFDIPLVVRNDYLSHNGIKTHDDVLNELVKFFNYEEQVKLQYKYSVTGNKN
                     TAISDQVSVVNSGTADTPLIVEARAIKSSSYFMITKNDEDYFMVGDDEVTKEVKDYMP
                     PVYHSEFRDFKGWTKMITEDIPSNDLGGKVGGDFVISNLGEGYKATNFPDAKGWVGAG
                     TKRGLPKAMTDFQITYKCIVEQKGKGAGRTAQHIYDSDGKLLASIGYENKYHDRKIGH
                     IVVTLYNQKGDPKKIYDYQNKPIMYNLDRIVVYMRLRRVGNKFSIKTWKFDHIKDPDR
                     RKPIDMDEKEWIDGGKFYQRPASIIAIYSAKYNGYKWMEMNGLGSFNTEILPKPKGAR
                     DVIIQKGDLVKIDMQAKSVVINEEPMLSEKSFGSNYFNVDSGYSELIIQPENVFDTTV
                     KWQDRYL"
     gene            15753..19538
                     /locus_tag="POB55_000883"
     CDS             15753..19538
                     /locus_tag="POB55_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499858.1"
                     /translation="MIHVLDFNDKIIDFLSTDDPSLVRAIHKRNVNDNSEMLELLISS
                     ERAEKFRKRHRVIIRDSNKQWREFIINWVQDTMDGYTEIECIASYLADITTAKPYAPG
                     KFEKKTTSEALKDVLSDTGWEVSEQTEYDGLRTTSWTSYQTRYEVLKQLCTTYKMVLD
                     FYIELSSNTVKGRYVVLKKKNSLFKGKEIEYGKDLVGLTRKIDMSEIKTALIAVGPEN
                     DKGKRLELVVTDDEAQSQFNLPMRYIWGIYEPQSDDQNMNETRLSSLAKTELNKRKSA
                     VMSYEITSTDLEVTYPHEIISIGDTVRVKHRDFNPPLYVEAEVIAEEYNIISENSTYT
                     FGQPKEFKESELREEFNKRLNLIHQKLNDNISNINTIVKDIVDGELEYFERKIHKSDT
                     PPENPVNDTLWYDTSNPDVAVLRRYWNGRWIEATPNDVEKLGGITREKALFSELNNIF
                     INLSIQHASLLSEATELLNSEYLVDNDLKADLQASLDAVIDVYNQIKNNLESMTPETA
                     TIGRLVDTKTLFLEYRKKLQDVYTDVEDVKIAISDRFKLLQSQYTDEKYKEALEIIAT
                     KFGLTVNEDLQLVGEPNVVKSAIEAARESTKEQLRDYVKTSDYKTDKDGIVERLDTAE
                     AERTTLKGEIKDKVTLNEYRNGLEEQKQYTDDQLSDLSNNPEIKASIEQANQEAQEAL
                     KSYIDAQDDLKEKESQAYADGKISEEEQRAIQDAQAKLEEAKQNAELKARNAEKKANV
                     YTDNKVKESTDAQRKTLTRYGSQIIQNGKEIKLRTTKEEFNATNRTLSNILNEIVQNV
                     TDGTTIRYDDNGVAQALNVGPRGIRLNADKIDINGNREINLLIQNMRDKVDKTDIVNS
                     LNLSREGLDINVNRIGIKGGDNNRYVQIQNDSIELGGIVQRTWRGKRSTDDIFTRLKD
                     GHLRFRNNTAGGSLYMSHFGISTYIDGEGEDGGSSGTIQWWDKTYSDSGMNGITINSY
                     GGVVALTSDNNRVVLESYASSNIKSKQAPVYLYPNTDKVPGLNRFAFTLSNADNAYSS
                     DGYIMFGSDENYDYGAGIRFSKERNKGLVQIVNGRYATGGDTTIEAGYGKFNMLKRRD
                     GNRYIHIQSTDLLSVGSDDAGDRIASNSIYRRTYSAAANLHITSAGTIGRSTSARKYK
                     LSIENQYNDRDEQLEHSKAILNLPIRTWFDKAESEILARELREDRKLSEDTYKLDRYV
                     GLIAEEVENLGLKEFVTYDDKGEIEGIAYDRLWIHLIPVIKEQQLRIKKLEESKNAG"
     gene            19528..19680
                     /locus_tag="POB55_000884"
     CDS             19528..19680
                     /locus_tag="POB55_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499859.1"
                     /translation="MQDNKQGLQANPEYTIHYLSQEIMRLTQENAMLKAYIQENKENQ
                     QCAEEE"
     gene            19727..20014
                     /locus_tag="POB55_000885"
     CDS             19727..20014
                     /locus_tag="POB55_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499860.1"
                     /translation="MAKEIINNTERFILVQIDKEGTERVVYQDFTGSFTTSEMVNHAQ
                     DFKSEENAKKIAETLNLLYQLTNKKQRVKVVKEVVDRTDLSSDKTVDSETM"
     gene            20070..20444
                     /locus_tag="POB55_000886"
     CDS             20070..20444
                     /locus_tag="POB55_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499861.1"
                     /translation="MDDIQKIKKELSELVERVDDVEILANETADHVLELREEHKQHHN
                     ELRESHKELKDKQDKVVDENLEQTKILNRIEERYQTQVDVAQKNEEKTLAQNKWLVGA
                     IWALVTIVMIAVITASITALLP"
BASE COUNT         7726 a         2818 c         4305 g         5849 t
ORIGIN      
        1 agttttatca cttcagagta ccgacgaaaa ataaacgatt ataatcttta tcttgataaa
       61 tttgaagaac agtttagtca aaagtttaaa ataaacagaa cttcgataaa agaaagaatt
      121 attattaatt taaacaagag gagatttaaa tgatgtggat tactatgact attgtatttg
      181 ctatattgct attagtttgt atcagtatta atagtgatcg tgcaagggag atacaagcgc
      241 tcagatatat gaatgattat ctacttgatg aagtagttaa aactaaagga tacaacgggt
      301 taaaagaata caggattgaa ttaaagcgaa tgaataacga tattaaaaag taatttatat
      361 tatcggaggt attgcattga atgataaaga ttgagaaaca cgatatcaaa aagcttgaag
      421 aatacattca gcacatcgat aactatcgaa gagagttgaa gatgcgagaa tatgaattac
      481 ttgaaagtca tgaaccagat aatgcgggag ctggcaaaag taatttgccg ggtaacccga
      541 ttgaacgatg tgcaataaag aagtttagtg ataacaggta caatacatta agaaatatag
      601 ttaacggtgt agatagattg atagatgaaa gtgatgagga tacgcttgag ttattaaggt
      661 ttagatattg ggattgtcct attggttgtt atgaatggga agatatagca cattactttg
      721 gtacaagtaa gacaagtata ttacgtagaa ggaatgcact gatcgataag ttagcaaagt
      781 atattggtta tgtgtagcgg acttttaccc tatgtaagtc cgcattaaaa cagtttatta
      841 tgttagtatc agattaatat ttaaagttat taaatgctaa tacgacgcat gaacaagagg
      901 cgcatcacta tgtgatgtgt ctttttattt atgaggtatg aacatgttca aactaatagt
      961 aaatacatta ctacacatca agtatagatg cgtcttgata ctacttaagt tatataaggt
     1021 gaaacattat gatgactaaa gacgaacgca tacgattcta taagtctaaa gaatggcaaa
     1081 caacaagaaa aagagtgcta gaaagagata attatgaatg tcaacaatgt aagagagacg
     1141 gcaagttaac gacatatgac aaaagaaagc gtaagtcgtt ggatgtagat catatattat
     1201 cgctagaaca tcatccggag tttgctcatg acttaaacaa tttagaaaca ctgtgtatta
     1261 aatgtcacaa caaaaaagaa aagagattta taaaaaaaga aaataaatgg aaagacgaaa
     1321 aatggtaaat acccccgggt caaaaaaatc aaaagcgatc aaaatacttg gggaacgggc
     1381 aggggctcga cttcgcgata attttaaaaa tccatgtata acccccctct tataaccatt
     1441 ttaaggcagg tgatgaaatg gagattatag ttgatgaaaa cttagtgctt aaagaaaaag
     1501 aaaggctgca agtattatat aaagacatac ctagcaataa attaaaagta gttgatggtt
     1561 taattattca agcagcaagg ctacgtgtaa tgcttgatta catgtgggaa gacataaaag
     1621 aaaaaggtga ctatgattta tttactcaat ctgaaaaggc gccaccatat gaaagggaaa
     1681 gaccagtagc caaactattt aatgctagag atgctgcata tcaaaaaata atcaaacaat
     1741 tatcggattt attgcccgaa gagaaagaag acacagaaac gccatctgat gattacctat
     1801 gattagtaat aaatacgttg atgaatatat aaatttgtgg aaacaaggaa agataatttt
     1861 aaataaagaa agaattgatc tctttaatta tctacaaaaa catatatatt cacgagatga
     1921 tgtatatttt gatgaacaga aaatcgagga ttgtatcaaa tttattgaaa aatggtattt
     1981 tccaacatta ccatttcaaa ggtttatcat agctaatata tttcttatag ataaaaatac
     2041 agatgaagct ttctttacag aatttgctat tttcatggga cgtggaggcg ggaaaaacgg
     2101 tctaataagt gctattagtg attttctttc tacgccctta cacggagtta aagaatatca
     2161 catctccatt gttgctaata gtgaagatca agcaaaaaca tcgtttgatg aaatcagaac
     2221 cgttttaatg gataacaaac gaaataagac gggtaaaacg ccaaaagctc cttatgaagt
     2281 tagtaaagca aaaataataa accgtgcaac taaatcggtt attcgatata acacatcaaa
     2341 cacaaaaacc aaagacggtg gacgtgaggg gtgtgttatt tttgatgaaa ttcattattt
     2401 ctttggtcct gaaatggtaa acgtcaaacg tggtggatta ggtaaaaaga aaaatagaag
     2461 aacgttttat ataagtactg atggttttgt tagagagggt tatatcgatg caatgaagca
     2521 caaaattgca agtgtattaa gtggcaaggt taaaaatagt agattgtttg ctttttattg
     2581 taagttagac gatccaaaag aagttgatga cagacagacg tgggaaaagg cgaacccaat
     2641 gttacataaa ccgttatcag aatacgctaa aacactgcta agtacgattg aagaagaata
     2701 taacgattta ccattcaacc gttcaaataa gcccgaattc atgactaagc gaatgaattt
     2761 gcctgaagtt gaccttgaaa aagtaatagc accatggaaa gaaatactag cgactaatag
     2821 agagatacca aatttagata atcaaatgtg tattggtggt ttagactttg caaacattcg
     2881 agattttgca agtgtagggc tattattccg aaaaaacgat gattacattt ggttaggaca
     2941 ttcgtttgta agacaagggt ttttggatga tgtcaaatta gaacctccta ttaaagaatg
     3001 ggaaaaaatg ggattattga ccattgtcga tgatgatgtc attgaaattg aatatatagt
     3061 tgattggttt ttaaaggcta gagaaaaata tgggcttgaa aaagtcatag ctgataatta
     3121 tagaactgat attgtaagac gtgcgtttga ggatgctggc ataaaacttg aagtacttag
     3181 aaatccaaaa gcaatacatg gattacttgc accacgtatc gatacaatgt ttgcgaaaca
     3241 taacgtaata tatggagaca atcctttgat gcgttggttt actaataatg ttgcagtaaa
     3301 ggttaaaccc gatggtaata aagaatatat taaaaaagat gaaaatagaa gaaaaaccga
     3361 tgggttcatg gcttttgttc acgcattata tagagcagac gatatagtag acaaagacat
     3421 gtctaaagcg cttgatgcat taatgagtat agatttctaa tagaggaggt gagacatgag
     3481 tattctagaa aagatattta aaactaggaa agatataaca tatatgcttg atttagatat
     3541 gatagaagat ctatcacaac aagcgtatgt gaaacgttta gcgattgata gttgtattga
     3601 atttgttgcg cgagctgtcg ctcaaagtca ttttaaagta ttggaaggta atagaattca
     3661 aaagaatgat gtttactaca agttaaatat aaaaccaaat actgacttat caagcgatag
     3721 tttttggcaa caagttatat ataaactaat ttatgataac gaggttttaa tcgtagtaag
     3781 tgacagcaaa gaattactta tcgcagatag cttttacaga gaagagtacg ctttgtatga
     3841 tgatatattc aaagatgtaa cggttaaaga ttatacttat caacgtactt tcacaatgca
     3901 agaggtcata tatttaaagt acaacaacaa taaagtgaca cactttgtag aaagtctatt
     3961 cgaagattac gggaaaatat tcggaagaat gataggtgca caattaaaaa actatcaaat
     4021 aagagggatt ttgaaatctg cctctagcgc atatgacgaa aagaatatag aaaaattaca
     4081 agcgttcaca aataaattat tcaatacttt taataaaaat caactagcaa tcgcgccttt
     4141 gatagaaggt tttgattatg aggaattatc taatggtggt aagaatagta acatgccttt
     4201 ttctgaattg agtgagctaa tgagagatgc aataaaaaat gttgtgttga tgattggtat
     4261 acctccaggt ttgatttacg gagaaacagc tgatttggaa aaaaacacgc ttgtatttga
     4321 gaagttctgt ttaacacctt tattaaaaaa gattcagaac gaattaaacg cgaaactcat
     4381 aacacaaagc atgtatttga aagatacaag aatagaaatt gtcggtgtga ataaaaaaga
     4441 cccacttcaa tatgctgaag caattgacaa acttgtgagt tctggttcat ttacaaggaa
     4501 tgaggtgcgg attatgttag gtgaagaacc atcagacaat cctgaattag acgaatacct
     4561 gattactaaa aactacgaaa aagctaacag tggtgaaaat gatgaaaaag aaaaagatga
     4621 aaacactttg aaaggtggtg atgaagatga aagcggagat taaaggcgtc atcgtttcca
     4681 acgaagataa atgggtttac gaaatgcttg gtatggattc gacttgtcct aaagatgttt
     4741 taacacaact agaatttagt gatgaagatg ttgatattat aattaactca aatggtggta
     4801 acctagtagc tggtagtgaa atatatacac atttaagagc tcataaaggc aaagtgaatg
     4861 ttcgtatcac agcaatagca gcaagtgcgg catcgcttat cgcaatggct ggtgaccaca
     4921 tcgaaatgag tccggttgct agaatgatga ttcacaatcc ttcaagtatt gcgcaaggag
     4981 aagcgaaaga tctaaatcat gctgcagaaa cattagaaca tgttggtcaa ataatggctg
     5041 aggcatatgc ggttagagct ggtaaaaaca aacaagaact tgtagaaatg atggctaggg
     5101 aaacgtggct aaatgctgat gaagccattg aacaaggttt tgcggatagt aaaatgtttg
     5161 aaaacgacaa tatgcaaatt gtagcaagca atacacaagt gttatcgaaa gatgtattaa
     5221 atcgtgtaac agctttggta agtaaaacgc cagaggttaa cattgatatt gacgcaatag
     5281 caaataaagt aattgaaaaa ataaatatga aagaaaagga atcagaaatc gatgttgcag
     5341 atagtaaagt atcagcaaat ggattttcaa gattcctttt ttaatacaaa aaataggagg
     5401 tcataaaatg actataaatt tatcggaaac attcgcaaat gcgaaaaacg aatttattaa
     5461 tgcagtaaac aacggtgaac cgcaagaaag acaaaatgaa ttgtacggtg acatgattaa
     5521 ccaactattt gaagaaacta aattacaagc aaaagcagaa gctgaaagag tttctagttt
     5581 acctaaatca gcacaatctt tgagtgcaaa ccaaagaagt ttcttcatgg atatcaataa
     5641 aaacgttaac tataaagaag aaaaactttt gccagaagaa acaattgata gaatttttga
     5701 agatttgacg acgaatcatc cgttattagc tgatttaggt attaaaaacg ctggtttgcg
     5761 tttgaagttc ttaaaatctg aaacttctgg cgtagccgtt tggggtaaaa tctatggtga
     5821 aattaaaggt caattagatg ctgcgttcag tgaagaaaca gcaattcaaa ataaattgac
     5881 agcgtttgtt gttttaccaa aagatttaaa tgattttggt cctgcgtgga ttgaaagatt
     5941 tgttcgtgtt caaatcgaag aagcatttgc agtggcgctt gaaactgcgt tcttaaaagg
     6001 tactggtaaa gaccaaccaa tcggcttaaa ccgtcaagta caaaaaggtg tatcggtaac
     6061 tgagggtgct tatccagaga aagaagaaca aggtacgctt acatttgcta atccgcgcgc
     6121 tacggttaat gaattgacgc aagtgtttaa ataccactca actaacgaga aaggtaaatc
     6181 agtagcggtt aaaggtaatg taacaatggt tgttaatccg tccgatgctt ttgaggttca
     6241 agcacagtat acacatttaa atgcaaatgg cgtatatgtt actgctttac catttaattt
     6301 gaatgttatc gagtctacag tccaagaagc aggtaaggtt ttaacgtacg ttaaaggttt
     6361 atatgatggt tatttagctg gtggtattaa tgttcagaaa tttaaagaaa cacttgcgtt
     6421 agatgatatg gatttataca ctgcaaaaca atttgcttac ggcaaagcga aagataataa
     6481 agttgctgct gtttggaaat tagatttaaa aggacataag ccagctttag aaggtaccga
     6541 agaaacacta taaaatttta tgaggtgata aaatggtgaa atttaaagtt gttagagctt
     6601 ttaaagacat agagcacaat caacacaagt acaaagtagg ggagttgtat ccagctgaag
     6661 ggtataacaa tcctcgtgtt gaattgttga caaatcaaat caaaaataag tacgacaaag
     6721 tttatatcgt acctttagat aagctgacaa aacaagaatt attagaacta tgcgaatcat
     6781 tacaaaaaaa agcgtctagt tcaatggtta aaagtgaaat cgtcgactta ttgaatggtg
     6841 aagacaatga cgattgatga tttgcttgtc aaatttaaat cacttgaaaa gattgaccat
     6901 aattcagagg atgagtactt aaagcagttg ttaaaaatgt cgtacgagcg tataaaaaat
     6961 cagtgcggag tttttgaatt agagaattta ataggtcaag aattgatact tatacgcgct
     7021 agatatgctt atcaagattt attagaacac ttcaacgata attacagacc tgaaataata
     7081 gatttttcgt tatctctaat ggaggtatca gaagatgaag aaagtgttta aaaaacctag
     7141 aattacaact aaacgtttaa atactcgtgt tcatttttat aagtatactg aaaataatgg
     7201 tccagaagct ggagaaaaag aagaaaaatt attatatagc tgttgggcga gtattgatgg
     7261 tgtctggtta cgtgaattag aacaagctat ctcaaacgga acccaaaatg acattaaatt
     7321 gtatattcgt gatccgcaag gtgattattt acccagtgaa gaacattatc ttgaaattga
     7381 atcaagatat ttcaaaaatc gtttgaatat aaagcaagta tcaccagatt tggataataa
     7441 agactttatt atgattcgtg gaggatatag ttcatgagtg tgaaagtgat aggtgataaa
     7501 gcattagaaa gagaattaga aaaacgtttt ggcataaaag agatggtaaa agttcaagat
     7561 aaggcgttaa tagctggtgc taaggtaatt gttgaagaag taaaaaaaca actaaagccc
     7621 tcaaaagata cgggagcatt aattaatgag gtaagtttta gtaaacctga atggataaac
     7681 ggaaaacgta caattactgt tcattggcga ggttctaaag accgttataa aatcgtacat
     7741 ttaattgaat atggacacgt tcaaaaagga acaggtaaat ttatcaaacc taaagctatg
     7801 ggcggtgtta atagagcaat aagacaaggg caaaataagt attttgagac gctaaaaagg
     7861 gagttgaaaa aattgtgatt gatattttgt acaaagttca tgaagtgatt agtcaagaca
     7921 gaattattag agagcacgta aatatcaata atattaagtt caataaatac cctaatgtaa
     7981 aagatactga tgtacctttt attgttattg acgatatcga cgacccaata cctacaactt
     8041 atactgacgg agatgagtgt gcatatagtt atattgtcca aatagatgtt tttgttaagt
     8101 acaatgatga atataatgcg agaatcataa gaaataagat atctaatcgc attcaaaagt
     8161 tattatggtc tgaactaaaa atgggaaatg tttcaaatgg aaaaccggaa tatatagaag
     8221 aatttaaaac atatagaagc tctcgcgttt acgagggcat tttttataag gaggaaaatt
     8281 aaatggcagt aaaacatgca agtgcgccaa aggcgtatat taacattact ggtttaggtt
     8341 tcgctaaatt aacgaaagaa ggcgcggaat taaaatatag tgatattaca aaaacaagag
     8401 gattacaaaa aattggtgtt gaaactggag gagacttgaa aacagcatat gctgatggtg
     8461 gtccaatcga atcagggaac acagacggag aaggtaaaat ttcgttacaa atgcatgctt
     8521 tccctaaaga gattcgcaaa atagtattca atgaagagta tgatgaagac ggtgtttata
     8581 aagaaactca aggtaaacaa aacaactatg tagcaatttg gttcagacaa gagcgtcgag
     8641 acggcacatt tagaacggtt ttattaccta aagtcatgtt tacaaatcct aaaatcgatg
     8701 gagaaacggc tgagaaagat tgggatttct caagtgaaga ggttgaaggt gaggcacttt
     8761 tccctttagt tgataataaa aagtcagtac gtaagtatat ctttgattca gctaacatga
     8821 caaatcatga tggagacggt gaaaaaggcg aagaggcttt cttaaagaaa attttaggcg
     8881 aagaatatac tggaaacgtg acagagggta acgaagaaac tttgtaacaa aaccggcttc
     8941 atcggaaact gcggtaaagt cggttaatat accagatagc attaaaacac ttaaagttgg
     9001 cgacacatac gatttaaatg ttgtagtaga gccatctaat caaagtaagt tattgaaata
     9061 cacaacagat caaacgaata ttgtatcaat caatagtgat ggtcaagtta ctgcggaagc
     9121 acaaggcatt gctacggtta aagcaacagt tggtaatatg agtgacacta taacaataaa
     9181 tgtagaagca taagaggggg caacccctct attttatttg aaaataagga gagtattata
     9241 aaatggcaaa attaaaacgt aacattattc aattagtaga agacccgaaa gcaaatgaaa
     9301 ttaaattaca aacgtactta acaccacact tcatttcatt tgaaattgta tacgaagcaa
     9361 tggatttaat cgatgatatt gaggacgaaa atagcacgat gaaaccaaga gaaatcgctg
     9421 acagattgat ggatatggtt gtaaaaattt acgataacca attcacagtt aaagacctaa
     9481 aagaacgtat gcatgcacct gatggaatga atgcacttcg tgaacaagtg attttcatta
     9541 ctcaaggtca gcaaactgag gaaactagaa attttatcca gaacatgaaa taaagcctga
     9601 agatttaaca tataaagcaa tgttgaaaaa tatggatact ctcatgatgg acttaattga
     9661 aaatggtaaa gacgctaacg aagttttaaa aatgccattt cattatgtac tttccatata
     9721 tcaaaataaa aacaatgaca tttctgaaga aaaagcagag gctttaattg atgcgtttta
     9781 accttaaccg tttggttagg gttatttttt tgaacttttt tagaaaggag gtaaaaaatg
     9841 ggagaaagaa taaaaggttt atctataggt ttggatttag atgcagcaaa tttaaataga
     9901 tcatttgcag aaatcaaacg aaactttaaa actttaaatt ctgacttaaa attaaccggt
     9961 aacaacttca aatataccga aaaatcaact catagttaca aacaaaggat taaagaactt
    10021 gatggaacta tcacaggtta taagaaaaac gttgatgatt tagccaagca atatggcaag
    10081 gtatctcaag aacagggcga aaacagcgcg gaagctcaaa aattacgaca agaatataac
    10141 aaacaagcaa atgagctgaa ttttttagaa aaagaactag aaaaaacaac aactgagttt
    10201 gaagagttca aaaaagctca agttgaagct caaagaatgg cagaaagtgg ctggggaaaa
    10261 accagtaaag tttttgaaag tatgggacct aaattaacaa aaatgggtga tggtttaaaa
    10321 tccattggta aaggtttgat gattggtgta actgcacctg ttttaggtat tgcagcagca
    10381 tcaggaaaag cttttgcaga agttgataaa ggtttagata cagttaccca agcaacagga
    10441 gcaaccggcg gagagcttaa gaagttgcag aattcattta aagatgttta tggcaacttt
    10501 ccagcagacg ctgagactgt aggcggtgtt ttaggggaag ttaacacaag gttaggtttc
    10561 actggcaaag aacttgagag tgccacagag tcattcttga aatttagtca cataacaggt
    10621 tctgacggcg tacaagccgt tcaattaatt acgcgtgcaa tgggtgatgc aggtattgaa
    10681 gctgatgagt atcaaagtgt acttgatatg gtagcgaaag cagcacaggc tagcggtata
    10741 agtgttgata cattagctga tagcattact aaatacggtg ctccaatgag ggctatgggc
    10801 tttgagatga aagaatcaat cgctttattc tctcaatggg agaaatcagg tgttaatact
    10861 gaaatagcct tcagtggttt gaaaaaagct atatccaatt ggggtaaagc gggtaaagac
    10921 ccaagagaag aatttaagaa gacattagca gaaattgaaa ggacaccgga tatagctagc
    10981 gcaacaagtt tagcgattga agcatttggt gcaaaagcag gtcctgattt agcagatgct
    11041 attaaaggcg gtcgctttag ttaccaagag ttcttaaaaa ctatcgaaga ttcgcaagga
    11101 acggtcaatc agacatttaa agattctgaa agtggctccg aaagatttaa agtagcaatg
    11161 aataaactta aattagtagg tgctgatgta tgggcttcta ttgaaagtgc gtttgctcca
    11221 gtcatggaag aattaatcaa aaagctatct gtagcagttg attggttttc aagtttaagt
    11281 gatggatcta aaaggtcgat tgttatattc ggtggtattg ctgctgcaat tggtcctgta
    11341 gtttttggat taggtgcatt cataagcaca attggcaacg cagtaactgt attagctcca
    11401 ttattagcta gtattgtaaa ggctggcgga ttgattagtt ttttatcaac taaagtgcct
    11461 attttaggaa cagtcttcac agcattaact ggtccaattg gtatcgtgtt aggtgtactg
    11521 gctggtttag cagtcgcatt tacaatagct tataagaaat ctgaaacatt cagaaatttt
    11581 gttaatggtg caattaacag tgttaaacaa acgtttagta atttcattca atttatccaa
    11641 cctttcattg attccgttaa aaacgtcttt aaacaagcgg tttcagcaat cgttgatttc
    11701 gctaaagata tttggagtca aattaatgga ttctttaatg aaaacggaat ttctattgtt
    11761 caagcgcttc aaaatatatg caattttatc aaagctatat ttgaatttat cttaaatttt
    11821 gtaattaaac caatcatgtt tgcgatttgg caagtgatgc aatttatttg gccggcggtt
    11881 aaagccttga ttgtcagtac ttgggagaat ataaaaggag taatacaagg tgctttaaat
    11941 atcatacttg gctttattaa gttcttttca agtttattca ctggtaattg gcgaggtgtt
    12001 tgggacggta ttgtgatgat actaaaaggc actgtgcagt taatttggaa tttaatacaa
    12061 ctgtggtttg taggtaagat tctaggtgtt gttagatact ttggtggatt gcttaaaggt
    12121 ttaatatccg gtatctgggg tgttatcaaa ggtattttca caaaatcatt atctgcaatt
    12181 tggaatgcaa cgaaaagtat ttttggtttc ttatacaata gtgttaaatc tattttcact
    12241 aatatgaaaa actggttatc tagtacgtgg aataatatca aaagcaatac cgtcggcaag
    12301 gctcattcgt tatttacggg tgtaaggtct aaattcacaa gtttatggaa tgcgacgaaa
    12361 gatatattta ctaaattaag aaattggatg tcaaacatct ggaactctat taaagataac
    12421 acggtaggta tagcgggtcg cttatgggat agagtgcgta acatctttgg aagcatgcgt
    12481 gacggtttaa aatctatcat tagtaaaatt aaagatcata tcggtggtat ggtagacgct
    12541 gttaaaagag gtcttaataa attaattgaa ggtttaaact gggtcggtgg taagttgggt
    12601 atggacaaaa taccgaagtt acacactggt actgaacata cacatactac tacaagatta
    12661 gttaagaacg gtaagattgc acgtgacaca ttcgctacag ttggggataa gggacgcgga
    12721 aatggtccaa atggtttcag aaatgaaatg attgaattcc ctaatggtaa acgtgtaatc
    12781 acaccaaata cagatactac ggcttattta cctaaaggct caaaagtata caacggggca
    12841 caaacttatt caatgttaaa cggaacgctt ccgagattta gtttaggtac tatgtggaaa
    12901 gatattaaat ctggtgcatc atcggcattt aactggacaa aagataaaat aggtaaaggt
    12961 accaaatggc ttggcgataa agttggcgat gttttagatt ttatggaaaa tccaggcaaa
    13021 cttttaaatt atatacttga agcttttgga attgatttca attctttaac taaaggtatg
    13081 ggaattgcag gcgacataac aaaagctgca tggtctaaga ttaagaaaag tgctactgat
    13141 tggataaaag aaaatttaga agctatgggc ggtggcgatt tagtcggtgg aatattagac
    13201 cctgacaaaa ttaattatca ttatggacgt accgcagctt ataccgctgc aactggaaga
    13261 ccatttcatg aaggtgtcga ttttccattt gtatatcaag aagttagaac gccgatgggt
    13321 ggcagactta caagaatgcc atttatgtct ggtggttatg gtaattatgt aaaaattact
    13381 agtggcgtta tcgatatgct atttgcgcat ttgaaaaatt ttagcaaatc accacctagt
    13441 ggcacgatgg taaagcccgg tgatgttgtt ggtttaactg gtaataccgg atttagtaca
    13501 ggaccacatt tacattttga aatgaggaga aatggaagac attttgaccc tgaaccatat
    13561 ttaaggaatg ctaagaaaaa aggtaggtta tcaattggtg gcggtggcgc tacttctgga
    13621 agtggtgcaa cttatgccag ccgagtaatc cgacaagcac aaagtatttt aggaggacgt
    13681 tataaaggta agtggattca tgaccagatg atgcgagttg caaagcgcga aagtaactat
    13741 caatcaaatg cagtgaataa ttgggacatt aatgctcaaa gaggagaccc gtctagagga
    13801 ttattccaaa ttatcggctc aacttttaga gctaacgcta aacgagggta cactaattat
    13861 aataatccag tacatcaagg tatctcagca atgcagtaca ttgttagacg atatggttgg
    13921 ggtggtttta aacgtgctgg tgattacgca tatgctacag gtggaaaagt ttttgatggt
    13981 tggtataact taggtgaaga cggtcatcca gaatggatta ttccaacaga tccagctcgt
    14041 agaaatgatg caatgaagat tttgcattat gcagcagcag aagtaagagg gaaaaaagcg
    14101 agtaaaaata agcgtcctag ccaattatca gacttaaacg ggtttgatga tcctagctta
    14161 ttattgaaaa tgattgaaca acagcaacaa caaatagctt tattactgaa aatagcacaa
    14221 tctaacgatg tgattgcaga taaagattat cagccgatta ttgacgaata cgcttttgat
    14281 aaaaaggtga acgcgtctat agaaaagcga gaaaggcaag aatcaacaaa agtaaagttt
    14341 agaaaaggag gaattgctat tcaatgatag acactattaa agtgaacaac aaaacaattc
    14401 cttggttgta tgtcgaaaga gggtttgaaa taccctcttt taattatgtt ttaaaaacag
    14461 aaaatgtaga tggacgttcg gggtctatat ataaagggcg taggcttgaa tcttatagtt
    14521 ttgatatacc tttggtggta cgtaatgact atttatctca caacggtatt aaaacacatg
    14581 atgacgtctt gaatgaatta gtaaagtttt ttaactacga ggaacaagtt aaattacaat
    14641 acaaatattc agtaacagga aataaaaata ctgcgatttc agaccaagtt tcagttgtaa
    14701 atagtgggac tgctgacact cctttaattg ttgaagcccg agcaattaaa tcatctagtt
    14761 actttatgat cactaaaaat gatgaagatt attttatggt tggtgatgat gaggtaacca
    14821 aagaagttaa ggattacatg cctcctgttt atcatagtga gtttcgtgat ttcaaaggtt
    14881 ggactaagat gattactgaa gatattccaa gtaatgattt aggtggtaag gtcggcggtg
    14941 attttgtgat atccaatctt ggcgaaggat ataaagcaac taattttcct gatgcaaaag
    15001 gttgggttgg tgctggcacg aaacgagggc tccctaaagc gatgacagat tttcaaatta
    15061 cctataaatg tattgttgaa caaaaaggta aaggtgccgg aagaacagca caacatattt
    15121 atgatagtga tggtaagtta cttgcttcta ttggttatga aaataaatat catgatagaa
    15181 aaataggaca tattgttgtt acgttgtata accaaaaagg agaccccaaa aagatatacg
    15241 actatcagaa taaaccgata atgtataact tggacagaat cgttgtttat atgcggctca
    15301 gaagagtagg taataaattt tctattaaaa cttggaaatt tgatcacatt aaagacccag
    15361 atagacgtaa acctattgat atggatgaga aagagtggat agatggcggt aagttttatc
    15421 agcgtccagc ttctatcata gctatctata gtgcgaagta taacggttat aagtggatgg
    15481 agatgaatgg attaggttca ttcaatacgg agattctacc gaaaccgaaa ggcgcaaggg
    15541 atgtcattat acaaaaaggt gatttagtga aaatagatat gcaagcaaaa agtgttgtca
    15601 tcaatgagga accaatgttg agcgagaaat cgtttggaag taattatttc aatgttgatt
    15661 ctgggtacag tgaattaatc atacaacctg aaaacgtctt tgatacgacg gttaaatggc
    15721 aagatagata tttatagaaa ggagatgaga gtgtgataca tgttttagat tttaacgaca
    15781 agattataga tttcctttct actgatgacc cttccttagt tagagcgatt cataaacgta
    15841 atgttaatga caattcagaa atgcttgaac tgctcatatc atcagaaaga gctgaaaagt
    15901 tccgtaaacg acatcgtgtt attataaggg attcaaacaa acaatggcgt gaatttatta
    15961 ttaactgggt tcaagatacg atggacggct acacagagat agaatgtata gcgtcttatc
    16021 ttgctgatat aacaacagct aaaccgtatg caccaggaaa atttgagaaa aagacaactt
    16081 cagaagcatt gaaagatgtg ttgagcgata caggttggga agtttctgaa caaaccgaat
    16141 acgatggctt acgtactacg tcatggactt cttatcaaac tagatatgaa gttttaaagc
    16201 aattatgtac aacctataaa atggttttag atttttatat tgagcttagc tctaataccg
    16261 tcaaaggtag atatgtagta ctcaaaaaga aaaacagctt attcaaaggt aaagaaattg
    16321 aatatggtaa agatttagtc gggttaacta ggaagattga tatgtcagaa atcaaaacag
    16381 cattaattgc tgtgggacct gaaaatgaca aagggaagcg tttagagcta gttgtgacag
    16441 atgacgaagc gcaaagtcaa ttcaacctac ctatgcgcta tatttggggg atatatgaac
    16501 cacaatcaga tgatcaaaat atgaatgaaa cacgattaag ttctttagcc aaaacagagt
    16561 taaataaacg taagtcggca gttatgtcat atgagattac ttctactgat ttggaagtta
    16621 cgtatccgca cgagattata tcaattggcg atacagtcag agtaaaacat agagatttta
    16681 acccgccatt gtatgtagag gcagaagtta ttgctgaaga atataacata atttcagaaa
    16741 atagcacata tacattcggt caacctaaag agttcaaaga atcagaatta cgagaagagt
    16801 ttaacaagcg attaaaccta atacaccaaa aattaaacga caatattagc aatatcaata
    16861 ctatagtaaa agatattgta gatggtgaat tagaatactt tgaacgcaaa attcataaaa
    16921 gtgatacacc gccagaaaat ccagtcaatg atacgctttg gtatgataca agtaaccctg
    16981 atgttgctgt cttgcgtaga tattggaatg gtcgatggat tgaagcaaca ccaaatgatg
    17041 ttgaaaaatt aggtggtata acaagagaga aagcgctatt cagtgaatta aacaatattt
    17101 ttattaattt atctatacaa cacgctagtc ttttgtcaga agctacagaa ttactgaata
    17161 gcgagtactt agtagataat gatttgaaag cggacttaca agcaagttta gacgctgtga
    17221 ttgatgttta taatcaaatt aaaaataatt tagaatctat gacacccgaa actgcaacga
    17281 ttggtcggtt ggtagataca aaaactttat ttcttgagta tagaaagaaa ttacaagatg
    17341 tttatacaga tgtagaagat gtcaaaatcg ccatttcaga tagatttaaa ttattacagt
    17401 cacaatacac tgatgaaaaa tataaagaag cgttggaaat aatagcaaca aaatttggtt
    17461 taacggtgaa tgaagatttg cagttagtcg gagaacctaa tgttgttaaa tcagctattg
    17521 aagcagctag agaatccaca aaagaacaat tacgtgacta tgtaaaaaca tcggactata
    17581 aaacagacaa agacggtatt gttgaacgtt tagatactgc tgaagctgag agaacgactt
    17641 taaaaggtga aatcaaagat aaagttacgt taaacgaata tcgaaacgga ttggaagaac
    17701 aaaaacaata tactgatgac cagttaagtg atttgtccaa taatcctgag attaaagcaa
    17761 gtattgaaca agcaaatcaa gaagcgcaag aagctttaaa atcatacatt gatgctcaag
    17821 atgatcttaa agagaaggaa tcgcaagcgt atgctgatgg taaaatttcg gaagaagagc
    17881 aacgcgctat acaagatgct caagctaaac ttgaagaggc aaaacaaaac gcagaactaa
    17941 aggctagaaa cgctgaaaag aaagctaatg tttatacaga caacaaggtc aaagaaagca
    18001 cagatgcaca gaggaaaaca ttgactcgct atggttctca aattatacaa aatggtaagg
    18061 aaatcaaatt aagaactact aaagaagagt ttaatgcaac caatcgtaca ctttcaaata
    18121 tattaaacga gattgttcaa aacgttacag atggaacaac aatcagatat gatgataacg
    18181 gagtggctca agctttgaat gtggggccac gtggtattag attaaatgct gataaaattg
    18241 atattaacgg taatagagaa ataaaccttc ttatccaaaa tatgcgagat aaagtagata
    18301 aaaccgatat tgtcaacagc cttaatttat caagagaggg ccttgatatc aatgttaata
    18361 gaattggaat taaaggcggt gacaataaca gatatgttca aatacagaat gattctattg
    18421 aactaggtgg tattgtgcaa cgtacttgga gagggaaacg ttcaacagac gatattttta
    18481 cgcgactgaa agacggtcac ctaagattta gaaataacac cgctggcggt tcactttata
    18541 tgtcacattt tggtatttcg acttatattg atggtgaagg tgaagacggt ggttcatctg
    18601 gtacgattca atggtgggat aaaacttaca gtgatagtgg catgaatggt ataacaatca
    18661 attcctatgg tggtgtcgtt gcactaacgt cagataataa tcgggttgtt ctggagtctt
    18721 acgcttcatc gaatatcaaa agcaaacagg caccggtgta tttatatcca aacacagaca
    18781 aagtgcctgg attaaaccga tttgcattca cgctgtctaa tgcagataat gcttattcga
    18841 gtgacggtta tattatgttt ggttctgatg agaactatga ttacggtgcg ggtatcaggt
    18901 tttctaaaga aagaaataaa ggtcttgttc aaattgttaa tggacgatat gcaacaggtg
    18961 gagatacaac aatcgaagca gggtatggca aatttaatat gctgaaacga cgtgatggta
    19021 ataggtatat tcatatacag agtacagacc tactgtctgt aggttcagat gatgcaggag
    19081 ataggatagc ttctaactca atttatagac gtacttattc ggccgcagct aatttgcata
    19141 ttacttctgc tggcacaatt gggcgttcga catcagcgcg taaatacaag ttatctatcg
    19201 aaaatcaata taacgataga gatgaacaac tggaacattc aaaagctatt cttaacttac
    19261 ctattagaac gtggtttgat aaagctgagt ctgaaatttt agctagagag ctgagagaag
    19321 atagaaaatt atcggaagac acctataaac ttgatagata cgtaggtttg attgctgaag
    19381 aggtggagaa tttaggatta aaagagtttg tcacgtatga tgacaaagga gaaattgaag
    19441 gtatagcgta tgatcgtcta tggattcatc ttatccctgt tatcaaagaa caacaactaa
    19501 gaatcaagaa attggaggag tcaaagaatg caggataaca aacaaggatt acaagctaat
    19561 cctgaatata caattcatta tttatcacag gaaattatga ggttaacaca agaaaacgcg
    19621 atgttaaaag cgtatataca agaaaataaa gaaaatcaac aatgtgctga ggaagagtaa
    19681 tccttagcac tatttttata caaaaattta aggaggtcat ttaattatgg caaaagaaat
    19741 tatcaacaat acagaaaggt ttattttagt acaaatcgac aaagaaggta cagaacgtgt
    19801 agtatatcaa gatttcacag gaagttttac aacttctgaa atggttaacc atgctcaaga
    19861 ttttaaatct gaagaaaacg ctaagaaaat tgcggagacg ttaaatttgt tatatcaatt
    19921 aactaacaaa aaacaacgtg tgaaagtagt gaaagaagtt gtggatagaa ctgacttgtc
    19981 atctgataaa acagttgata gcgaaacaat gtagctatac taagctatga gcattacgct
    20041 catagctttc ttagaaagta ggtgtagttt tggatgatat tcagaaaata aaaaaagagc
    20101 tttctgaatt agttgaacgt gtggatgatg ttgaaatact agcaaatgaa acagctgatc
    20161 atgtgcttga acttagagag gaacataagc aacatcataa tgaactaaga gaatctcata
    20221 aagaacttaa agataagcaa gataaagttg tagatgagaa tttagagcaa acaaagatat
    20281 taaacagaat tgaagaaaga tatcaaacgc aagtagatgt tgcgcaaaaa aacgaagaaa
    20341 agacactcgc ccaaaataaa tggctcgtag gtgccatatg ggcgcttgta acaattgtta
    20401 tgattgcagt cattactgca tcaattactg cgttattacc ttaagggagg tggacaaaat
    20461 gagttgggca agatggttgt catgttattt gtatggtcgt aaatgtaaat aatgtttttg
    20521 gtcagtgctt cggcactggc tttttatttt gattgaaaag agttacgtac attgtattac
    20581 acagctcaaa agacaggaag catactgcaa gtgaagttgg gaagtgttgt caataccaag
    20641 taagtaagat atctgaaatg tataatagag taaaaatgaa atctttttat tatattat
//
LOCUS       DAMZKC010000032        20461 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.032, whole genome shotgun sequence.
ACCESSION   DAMZKC010000032 DAMZKC010000000
VERSION     DAMZKC010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 20461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20461
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.032"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            397..1308
                     /gene="rapZ"
                     /locus_tag="POB55_000887"
     CDS             397..1308
                     /gene="rapZ"
                     /locus_tag="POB55_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HDI6499862.1"
                     /translation="MDNNEKEKSKSELLVVTGLSGAGKSLVIQCLEDMGYFCVDNLPP
                     VLLPKFVELMEQGNPSLRKVAIAIDLRGKELFNSLVAVVDKVKSESDVIIDVMFLEAS
                     TEKLISRYKETRRAHPLMEQGKRSLINAINDEREHLSQIRSIANFVIDTTKLSPKELK
                     ERIRRYYEDEEFETFTINVTSFGFKHGIQMDADLVFDVRFLPNPYYVVDLRPLTGLDK
                     DVYNYVMKWKETEIFFEKLTDLLDFMIPGYKKEGKSQLVIAIGCTGGQHRSVALAERL
                     GNYLNEVFEYNVYVHHRDAHIESGEKK"
     gene            1305..2300
                     /locus_tag="POB55_000888"
     CDS             1305..2300
                     /locus_tag="POB55_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HDI6499863.1"
                     /translation="MRQIKVVLIGGGTGLSVMARGLREFPIDITAIVTVADNGGSTGK
                     IRDEMDIPAPGDIRNVIAALSDSESVLSQLFQYRFEENQISGHSLGNLLIAGMTNITN
                     DFGHAIKALSKILNIKGRVIPSTNTSVQLNAVMEDGEIVFGETNIPKKHKKIDRVFLE
                     PNDVQPMEEAIDALREADLIVLGPGSLYTSVISNLCVNGISDALIHSDAPKLYVSNVM
                     TQPGETDGYSVKDHIDAIHRQAGQPFIDYVICSTQTFNAQVLKKYEEKHSKPVEVNKA
                     ELEKESINVKTSSNLVEISENHLVRHNTKVLSTMIYDIALELISTIPFVPSDKRK"
     gene            2411..3355
                     /gene="whiA"
                     /locus_tag="POB55_000889"
     CDS             2411..3355
                     /gene="whiA"
                     /locus_tag="POB55_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HDI6499864.1"
                     /translation="MSFASEMKNELTRIDVDEMNAKAELSALIRMNGALSLSNQQFVI
                     NVQTENATTARRIYSLIKRVFNVEVEILVRKKMKLKKNNIYICRTKMKAKEILDELGI
                     LKDGIFTHEIDHSMIQDDEMRRSYLRGAFLAGGSVNNPETSSYHLEIFSQNESHAEGL
                     TKLMNSYELNAKHLERKKGSITYLKEAEKISDFLSLIGGYQALLKFEDVRIVRDMRNS
                     VNRLVNCETANLNKTVSAAMKQVESIKLIDKEIGIENLPDRLREIARIRVEHQEISLK
                     ELGEMVSTGPISKSGVNHRLRKLNDLADKIRNGEQIEL"
     gene            3928..4515
                     /gene="clpP"
                     /locus_tag="POB55_000890"
     CDS             3928..4515
                     /gene="clpP"
                     /locus_tag="POB55_000890"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDI6499865.1"
                     /translation="MNLIPTVIETTNRGERAYDIYSRLLKDRIIMLGSQIDDNVANSI
                     VSQLLFLQAQDSEKDIYLYINSPGGSVTAGFAIYDTIQHIKPDVQTICIGMAASMGSF
                     LLAAGAKGKRFALPNAEVMIHQPLGGAQGQATEIEIAANHILKTREKLNRILSERTGQ
                     SIEKIQKDTDRDNFLTAEEAKEYGLIDEVMVPETK"
     gene            complement(4758..5660)
                     /locus_tag="POB55_000891"
     CDS             complement(4758..5660)
                     /locus_tag="POB55_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HDI6499866.1"
                     /translation="MKQYLITGGTGMVGSQLVNEIKKSDSHITILTRHDQISNDKKIS
                     YVNWAKSGWEHKVPQNIDVVINLAGATLNKRWTPEYKQTLMLSRIQSTQALYELFKSR
                     NKAPKVLFNASATGYYPPDLFMSYTEVYKTLPFDFLSDIVYQWERFAQQFEQLGTRVV
                     IGRFGMILSNEGGALQTMKLPYKYYIGGRLGSGQQWYSWIHINDLIQAILFLINNESA
                     SGPFNLTAPIPERQNLFGYTLARAMHKPHETWAPSLAMRLILGQMSTVVLDTQKVLPN
                     KIQALGFQFKYSNLKMALEDLIKE"
     gene            6363..6992
                     /locus_tag="POB55_000892"
     CDS             6363..6992
                     /locus_tag="POB55_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4887 domain-containing protein"
                     /protein_id="HDI6499867.1"
                     /translation="MSNQNYDYNKNEDGSKKKMSTTAKVVSIATVLLLLGGLVFAIFA
                     YVDHSNKAKERMLNEQKQEQKEKRQKENAEKERKKKQQEEKEQNELDSQANQYQQLPQ
                     QNQYQYVPPQQQAPTKQRPAKEENDDKASKDESKDKDDNASQDKPDDNQKKTDDNKQP
                     AQPKPQPQQPTPKPNNNQQNNQSNQQAKPQAPQQNSQSTTNKQNNANDK"
     gene            7689..8702
                     /locus_tag="POB55_000893"
     CDS             7689..8702
                     /locus_tag="POB55_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HDI6499868.1"
                     /translation="MKDLLQAQQKLIPDLIDKMYKRFSILTAISKNQPVGRRSLSEHM
                     DMTERVLRSETDMLKKQDLIKVKPTGMEITAEGEQLISQLKGYFDIYADDNRLSEGIK
                     NKFQIKEVHVVPGDADNSQSVKTELGRQAGQLLEGILQEDAIVAVTGGSTMACVSEAI
                     HLLPYNVFFVPARGGLGENVVFQANTIAASMAQQAGGYYTTMYVPDNVSETTYNTLLL
                     EPSVINTLDKIKQANVILHGIGDALKMAHRRQSPEKVIEQLQHHQAVGEAFGYYFDTQ
                     GQIVHKVKTIGLQLEDLESKDFIFAVAGGKSKGEAIKAYLTIAPKNTVLITDEAAAKI
                     ILE"
     gene            8755..9765
                     /gene="gap"
                     /locus_tag="POB55_000894"
     CDS             8755..9765
                     /gene="gap"
                     /locus_tag="POB55_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDI6499869.1"
                     /translation="MAVKVAINGFGRIGRLAFRRIQEVEGLEVVAVNDLTDDDMLAHL
                     LKYDTMQGRFTGEVEVVDGGFRVNGKEVKSFSEPDASKLPWKDLNIDVVLECTGFYTD
                     KDKAQAHIEAGAKKVLISAPATGDLKTIVFNTNHQELDGSETVVSGASCTTNSLAPVA
                     KVLNDDFGLVEGLMTTIHAYTGDQNTQDAPHRKGDKRRARAAAENIIPNSTGAAKAIG
                     KVIPEIDGKLDGGAQRVPVATGSLTELTVVLEKQDVTVEQVNEAMKNASNESFGYTED
                     EIVSSDVVGMTYGSLFDATQTRVMSVGDRQLVKVAAWYDNEMSYTAQLVRTLAYLAEL
                     SK"
     gene            9904..11094
                     /locus_tag="POB55_000895"
     CDS             9904..11094
                     /locus_tag="POB55_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HDI6499870.1"
                     /translation="MAKKIVSDLDLKGKTVLVRADFNVPLKDGEITNDNRIVQALPTI
                     QYIIEQGGKIVLFSHLGKVKEESDKAKLTLRPVAEDLSKKLDKEVVFVPETRGEKLEA
                     AIKDLKEGDVLLVENTRYEDLDGKKESKNDPELGKYWASLGDVFVNDAFGTAHREHAS
                     NVGISTHLETAAGFLMDKEIKFIGGVVNDPHKPVVAILGGAKVSDKINVIKNLVNIAD
                     KIIIGGGMAYTFLKAQGKEIGISLLEEDKIDFAKDLLEKHGDKIVLPVDTKVAKEFSN
                     DAKITVVPSDSIPADQEGMDIGPNTVKLFADELEGAHTVVWNGPMGVFEFSNFAQGTI
                     GVCKAIANLKDAITIIGGGDSAAAAISLGFENDFTHISTGGGASLEYLEGKELPGIKA
                     INNK"
     gene            11216..11977
                     /locus_tag="POB55_000896"
     CDS             11216..11977
                     /locus_tag="POB55_000896"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HDI6499871.1"
                     /translation="MRTPIIAGNWKMNKTVQEAKDFVNALPTLPDSKEVESVICAPAI
                     QLDALTTAVKEGKAQGLEIGAQNTYFEDNGAFTGETSPVALADLGVKYVVIGHSERRE
                     LFHETDEEINKKAHAIFKHGMTPIICVGETDEERESGKANDVVGEQVKKAVAGLSEDQ
                     LKSVVIAYEPIWAIGTGKSSTSEDANEMCAFVRQTIADLSSKEVSEATRIQYGGSVKP
                     NNIKEYMAQTDIDGALVGGASLKVEDFVQLLEGAK"
     gene            11980..13497
                     /locus_tag="POB55_000897"
     CDS             11980..13497
                     /locus_tag="POB55_000897"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HDI6499872.1"
                     /translation="MAKKPTALIILDGFANRESEHGNAVKLANKPNFDRYYNKYPTTQ
                     IEASGLDVGLPEGQMGNSEVGHMNIGAGRIVYQSLTRINKSIEDGDFFENDVLNNAIA
                     HVNSHDSALHIFGLLSDGGVHSHYKHLFALLELAKKQGVEKVYVHAFLDGRDVDQKSA
                     LKYIEETEAKFNELGIGQFASVSGRYYAMDRDKRWEREEKAYNAIRNFDAPTYATAKE
                     GVEASYNEGLTDEFVVPFIVENQNDGVNDGDAVIFYNFRPDRAAQLSEIFANRAFEGF
                     KVEQVKDLFYATFTKYNDNIDAAIVFEKVDLNNTIGEIAQNNNLTQLRIAETEKYPHV
                     TYFMSGGRNEEFKGERRRLIDSPKVATYDLKPEMSAYEVKDALLEELNKGDLDLIILN
                     FANPDMVGHSGMLEPTIKAIEAVDECLGEVVDKILDMDGYAIITADHGNSDQVLTDDD
                     QPMTTHTTNPVPVIVTKEGVTLRETGRLGDLAPTLLDLLNVEQPEDMTGESLIKH"
     gene            13627..14931
                     /gene="eno"
                     /locus_tag="POB55_000898"
     CDS             13627..14931
                     /gene="eno"
                     /locus_tag="POB55_000898"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017466665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDI6499873.1"
                     /translation="MPIITDVYAREVLDSRGNPTVEVEVLTESGAFGRALVPSGASTG
                     EHEAVELRDGDKSRYLGKGVTKAVENVNEIIAPEIIEGEFSVLDQVSIDKMMIALDGT
                     PNKGKLGANAILGVSIAVARAAADLLGQPLYKYLGGFNGKQLPVPMMNIVNGGSHSDA
                     PIAFQEFMILPVGATTFKESLRWGTEIFHNLKSILSKRGLETAVGDEGGFAPKFEGTE
                     DAVETIIQAIEAAGYKPGEEVFLGFDCASSEFYENGVYDYSKFEGEHGAKRTAAEQVD
                     YLEQLVDKYPIITIEDGMDENDWDGWKQLTERIGDRVQLVGDDLFVTNTEILAKGIEN
                     GIGNSILIKVNQIGTLTETFDAIEMAQKAGYTAVVSHRSGETEDTTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDELFETAKYDGIKSFYNLDK"
     gene            15270..15728
                     /locus_tag="POB55_000899"
     CDS             15270..15728
                     /locus_tag="POB55_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499874.1"
                     /translation="MADRTNKEIKTGRFIATASIVFSILLIIHYFVSLDNATAKALLN
                     LTNQNTSDKAIDYILNSFRFTGIMYILAYLAGFITFWNRHTYVWWFMFAVYVSNSLFT
                     LINLSITIQAIKAAHGAYLTLPILIVIIGSVALAIYMLVVSIKRKSTFNR"
     gene            15795..16028
                     /gene="secG"
                     /locus_tag="POB55_000900"
     CDS             15795..16028
                     /gene="secG"
                     /locus_tag="POB55_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HDI6499875.1"
                     /translation="MHTFLIVLLIIDCIALITVVLLQEGKSSGLSGAISGGAEQLFGK
                     QKQRGVDLFLNRLTIILSILFFVLMICISYLGM"
     gene            16144..16884
                     /locus_tag="POB55_000901"
     CDS             16144..16884
                     /locus_tag="POB55_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase"
                     /protein_id="HDI6499876.1"
                     /translation="MQIKLPKPFFFEEGKRAVLLLHGFTGNSSDVRQLGRFLQKKGYT
                     SYAPQYEGHAAPPEEILKSSPFVWFKDALDGYDYLVEQGYDEIVVAGLSLGGDFALKL
                     SLNRDVKGIVTMCAPMGGKTEGAIYEGFLEYARNFKKYEGKNQETIDNEMDHFKPTET
                     LKELSEALDTIKDQVDEVFDPILVIQAENDNMIDPQSANYIYDHVDSDDKDIKWYSQS
                     GHVITIDKEKEQVFEDIYQFLESLDWSE"
     gene            16918..19290
                     /gene="rnr"
                     /locus_tag="POB55_000902"
     CDS             16918..19290
                     /gene="rnr"
                     /locus_tag="POB55_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HDI6499877.1"
                     /translation="MNLKQSIEEIINQPEYEPMSVSDFQDALGLSSADSFRDLIKVLV
                     ELEQSGLIERTKTDRYQKKHSSKGHSKLIKGTLSQNKKGFAFLRPEDEDMEDIFIPPT
                     KINRALDGDTVIVEIHQSKGEHKGKIEGEVKSIEKHSVTQVVGTYSEARHFGFVIPDD
                     KRIMQDIFIPKGQSLGAVDGHKVLVQITKYADGSDNPEGHISAILGHKNDPGVDILSI
                     IYQHGIEIEFPDEVLQEAEAVPDHIENTEIKGRHDLRDELTITIDGADAKDLDDAISV
                     KKLANGNTQLTVSIADVSYYVTEGSALDKEAYDRATSVYLVDRVIPMIPHRLSNGICS
                     LNPNVDRLTLSCRMEIDASGRVVKHEIFDSVIHSDYRMTYDAVNQIITEKDPNIREQY
                     KEITPMLDLAQDLSNRLIQMRKRRGEIDFDINEAKVLVNEDGIPTDVQLRQRGEGERL
                     IESFMLIANETVAEHFSKLDVPFIYRVHEQPKSDRLRQFFDFITNFGIMIKGTGEDIH
                     PTTLQKVQEEVEGRPEQMVISTMMLRSMQQAHYDDVNLGHFGLSAEYYTHFTSPIRRY
                     PDLTVHRLIRKYLIEKSMDNKEVKRWEDKLPELAEHTSKRERRAIEAERDTDELKKAE
                     YMIQHIGDEFEGIVSSVANFGMFIELPNTIEGMVHIANMTDDYYRFEERQMALIGERQ
                     AKVFRIGDTVKVKVTHVDVDERLIDFQIVGMPLPKNDRSQRPARGKTIQAKTRGKSLD
                     KSKSDDKGRKKKGSQRKGKNERKNDKSGNSKHKPFYKDKSVKKKARRKKK"
     gene            19312..19776
                     /gene="smpB"
                     /locus_tag="POB55_000903"
     CDS             19312..19776
                     /gene="smpB"
                     /locus_tag="POB55_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HDI6499878.1"
                     /translation="MAKKKSPGTLAENRKARHDYNIEDTIEAGIVLQGTEIKSIRRGS
                     ANLKDSYAQVKNGEMYLNNMHIAPYEEGNRFNHDPLRSRKLLLHKREIIKLGDQTREI
                     GYSIVPLKLYLKHGHCKVLLGVARGKKKYDKRQALKEKAVKRDVARDMKARY"
     gene            19867..20228
                     /gene="ssrA"
                     /locus_tag="POB55_000904"
     tmRNA           19867..20228
                     /gene="ssrA"
                     /locus_tag="POB55_000904"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         7292 a         2995 c         3970 g         6204 t
ORIGIN      
        1 gcacattatc gtaagctgac ttttcgtaag cttctgtgtt ggggccccgc caacttgcat
       61 tgtctgtaga aattcttttc gaaattctct gtgttggggc ccacacccca actcgcattg
      121 cctgtagaat ttcttttcga aattctctgt gttggggccc ctgactagag ttgaaaaaag
      181 cttgttgcaa gcgcgttttc attcagtcaa ctactagcaa tataatatta tagaccctag
      241 gacattgatt tatgtcccaa gctcctttta aatgatgtat atttttagaa atttaatcta
      301 gacatagttg gaaataaaaa taaaacatcg ttgcttaatt ttgtcataga acatttaaat
      361 taacatcatg aaattcgttt tggcggtgaa aaaataatgg ataataatga aaaagaaaaa
      421 agtaaaagtg aactattagt tgtaacaggt ttatctggcg caggtaaatc tttggttatt
      481 caatgtttag aagacatggg atatttttgt gtagataatc taccaccagt gttattacct
      541 aaatttgtag agttgatgga acaaggaaat ccatccttaa gaaaagtggc aattgcaatt
      601 gatttaagag gtaaggaact atttaattca ttagttgcag tagtggataa agttaaaagt
      661 gaaagtgacg tcatcattga tgttatgttt ttagaagcaa gtactgaaaa attaatttca
      721 agatataagg aaacgcgtcg tgcacatcct ttgatggaac aaggtaaaag atccttaatt
      781 aatgcaatta atgatgagcg agagcatttg tctcaaatta gaagtatagc taattttgtt
      841 atagatacta caaagttatc acctaaagaa ttaaaagaac gcattcgtcg atactatgaa
      901 gatgaagagt ttgaaacttt tacaattaat gtcacaagtt tcggttttaa acatgggatt
      961 cagatggatg cagatttagt atttgatgta cgatttttac caaatccata ttatgtagta
     1021 gatttaagac ctttaacagg attagataaa gacgtttata attatgttat gaaatggaaa
     1081 gagacggaga ttttctttga aaaattaact gatttgttag attttatgat acccgggtat
     1141 aaaaaagaag ggaaatctca attagtaatt gccatcggtt gtacgggtgg acaacatcga
     1201 tctgtagcat tagcagaacg actaggtaat tatctaaatg aagtatttga atataatgtt
     1261 tatgtgcatc atagggacgc acatattgaa agtggcgaga aaaaatgaga caaataaaag
     1321 ttgtacttat cggtggtggc actggcttat cagttatggc taggggatta agagaattcc
     1381 caattgatat tacggcgatt gtaacagttg ctgataatgg tgggagtaca gggaaaatca
     1441 gagatgaaat ggatatacca gcaccaggag acatcagaaa tgtgattgca gctttaagtg
     1501 attctgagtc agttttaagc caactttttc agtatcgctt tgaagaaaat caaattagcg
     1561 gtcactcatt aggtaattta ttaatcgcag gtatgactaa tattacgaat gatttcggac
     1621 atgccattaa agcattaagt aaaattttaa atattaaagg tagagtcatt ccatctacaa
     1681 atacaagtgt gcaattaaat gctgttatgg aagatggaga aattgttttt ggagaaacaa
     1741 atattcctaa aaaacataaa aaaattgatc gtgtgttttt agaacctaac gatgtgcaac
     1801 caatggaaga agcaatcgat gctttaaggg aagcagattt aatcgttctt ggaccagggt
     1861 cattatatac gagcgttatt tctaacttat gtgtcaatgg tatttcagat gcgttaattc
     1921 attctgatgc gcctaagcta tatgtttcta atgtgatgac gcaacctggg gaaacagatg
     1981 gttatagcgt gaaagatcat atcgatgcga ttcatagaca agctggacaa ccgtttattg
     2041 attatgtcat ttgtagtaca caaactttca atgctcaagt tttgaaaaaa tatgaagaaa
     2101 aacattctaa accagttgaa gttaataagg ctgaacttga aaaagaaagc ataaatgtaa
     2161 aaacatcttc aaatttagtt gaaatttctg aaaatcattt agtaagacat aatactaaag
     2221 tgttatcgac aatgatttat gacatagctt tagaattaat tagtactatt cctttcgtac
     2281 caagtgataa acgtaaataa tatagaacgt aatcatatta tgatatgata atagagctgt
     2341 gtaaaaaaat gaaaatagac agtggttcta aggtgaatca tgttttaaat aagaaaggaa
     2401 tgactgtacg atgagctttg catcagaaat gaaaaatgaa ttaactagaa tagacgtcga
     2461 tgaaatgaat gcaaaagcag agctcagtgc actgattcga atgaatggtg cacttagtct
     2521 ttcaaatcaa caatttgtta taaatgttca aacggaaaat gcaacaacgg caagacgtat
     2581 ttattcgttg attaaacgtg tctttaatgt ggaagttgaa atattagtcc gtaaaaaaat
     2641 gaagcttaaa aaaaataata tttatatttg tcgtacaaag atgaaagcga aagaaattct
     2701 tgatgaatta ggaattttaa aagacggcat ttttacgcat gaaattgatc attcaatgat
     2761 tcaagatgat gaaatgagac gcagttactt gagaggagct tttctggcag gtggctcagt
     2821 gaataaccct gaaacatctt cgtaccattt ggaaattttt tctcaaaatg agagtcatgc
     2881 agaaggctta acgaaactaa tgaatagtta tgaattgaat gccaaacatt tagagcgaaa
     2941 aaaaggaagt attacgtatt taaaagaagc ggaaaagatt tcggattttc ttagtttgat
     3001 aggtggctat caagcgttat taaaatttga agacgtacgt attgtaagag atatgcgtaa
     3061 ttctgttaac cgactcgtta attgtgaaac ggcaaatcta aataaaacag ttagtgctgc
     3121 gatgaaacaa gttgagagca ttaaattgat tgataaagaa attggtattg aaaatttacc
     3181 agacaggttg agagagattg ctagaattcg agtagaacat caagaaattt cgttgaaaga
     3241 gcttggagaa atggtatcaa ctggtccaat ttcaaaatca ggtgtaaatc accgattaag
     3301 aaaacttaat gatttagccg ataagattag aaatggtgaa caaatagaat tataagtaag
     3361 aaggtgtttt tggtagtgaa ttatcaaaaa cactttttta tgtttaaaaa gtgtttaatg
     3421 aataatagtc gactaactat taaattgagc tggaaagttt aatgaaggaa attcaataac
     3481 caagtgatgc ctaattttct cattaaatag catcatatgt atcaatttgg tcataagaaa
     3541 atatgaatgt gataattaat tgagtccctg aaagtccctg atcatagaca aaaacaaaat
     3601 cagcataaat aagaatcccg acattgcggg attctctgta ttgaaaagta ttgtatttta
     3661 ttaaaacagc ctccttgaag ggaattgaac ccctatctta agaaccggaa tcttacgtgt
     3721 tatccattac accacaagga gcaaatatgt aatcatatct ctgtactatg agaagtaaaa
     3781 taagtacaat ctaaaataga tattatcagt ttacaaagga aagagaaaag cgtcaaacaa
     3841 tgtaactatt taaagtcaaa gtgtttgacc aagtttgact taatatgtaa aataatgagt
     3901 aacagttatt acaaggagga aatatagatg aatttaattc ctacagttat tgaaacaaca
     3961 aaccgcggtg aacgtgcata tgatatatac tcacgtttat taaaagaccg tattattatg
     4021 ttaggttcac aaattgatga caacgtagca aattcaatcg tatcacagtt attattctta
     4081 caagcgcaag actcagagaa agatatttat ttatacatta attcaccagg tggaagtgta
     4141 acagctggtt ttgcgattta tgatacaatt caacacatta aacctgatgt tcaaacaatt
     4201 tgtatcggta tggctgcatc aatgggatca ttcttattag cagctggtgc aaaaggtaaa
     4261 cgtttcgcgt taccaaatgc agaagtaatg attcaccaac cattaggtgg tgctcaagga
     4321 caagcaactg aaatcgaaat tgctgcaaat cacattttaa aaacacgtga aaaattaaac
     4381 cgcattttat cagagcgtac tggtcaaagt attgaaaaaa tacaaaaaga cacagatcgt
     4441 gataacttct taactgcaga agaagctaaa gaatatggct taattgatga agtgatggta
     4501 cctgaaacaa aataattcaa agtaaagagt agactaagca gtctgctctt tttgtatgag
     4561 taaaccgagg tgtcaataaa ttgtttacta tactttgagc ggaaatatga ttgaatgaag
     4621 ctagttgaac cgtaactata tgaaatgttc ccttcaaagt agacattgaa aggaacattt
     4681 caatcctttg tttgtaagtc gctctagaca ttacatttag tacatatgtt gtttctaatg
     4741 ctcattaatg gtattgatta ttctttaatt aaatcttcaa gtgccatttt taaattacta
     4801 tatttaaatt ggaatcccaa tgcttgaatt ttattaggta atactttttg agtatccaat
     4861 actactgttg acatttgacc aagtatgaga cgcattgcaa gacttggtgc ccaagtttca
     4921 tggggcttat gcatagctct tgctaaagtg tagccaaata aattttgacg ctcaggtata
     4981 ggtgcagtta aattaaacgg accactagct gactcgttat ttattaaaaa taaaatagct
     5041 tgaattaaat cattgatatg aatccatgaa taccattgtt gaccagaacc taatctacca
     5101 ccaatgtaat atttgtatgg tagtttcatt gtttgtaacg caccgccttc attcgataaa
     5161 atcataccga aacgaccgat gacaactcgc gtacctaatt gttcaaattg ttgtgcgaaa
     5221 cgttcccatt gatacacaat atctgataag aaatcaaatg gtaaagtttt ataaacttct
     5281 gtgtaactca taaataaatc aggaggatag taaccagtgg cgctagcatt aaataaaact
     5341 ttaggtgctt tattacgtga tttaaacaat tcatataaag cttgcgtaga ttgaattcta
     5401 cttagcatta gcgtttgttt atattccggt gtccatcgtt tattcagtgt agcacctgct
     5461 aagttgatga ccacatcgat attttgagga actttgtgtt cccacccaga tttagcccag
     5521 ttgacatatg aaattttctt atcatttgaa atttggtcgt gtcgcgttaa tatcgtgata
     5581 tgtgaatctg attttttaat ttcattaact aattgagatc caaccatacc agtcccacca
     5641 gtaattaagt attgtttcat tatcattcac cccatgtaat tttgtattta gttactattt
     5701 aataatcatt attgttgttc aaaggttata cattatttag acaataatat gtcaataact
     5761 ttttttgaat tgtattttaa taaaaatgat ataagttata ataaaggtgt accttcgata
     5821 acgaataaac atctcttaaa agtatgtgta aaacgctgca tgatacaaac gaaggtaaaa
     5881 atttgactcc ctttagtagt ggacccgtac gttaatcgtg cgggtcgttt ttatttattt
     5941 cttattccca ttatacatca atttaaagca ttaatttttt aaacaaattt aagaatacat
     6001 agtaatataa caatctaaac ataaaaactt ttaacacaac acttaaacca atgctttaat
     6061 tttcaatacg tagctataat ttgttgtaaa atcaaaaagg ttaaaatgtt aattttcaaa
     6121 aaaaggctca aaatatgttt gatttagtta ttaaatgtta agatatataa gactactatt
     6181 tctttgtaaa aatgaatccg atttacgagt gagtaatagt gaaggcagtt ttaagttgaa
     6241 gaaggcaaaa agagtaaatg tttattaata tttgtagaaa ctaggtaagc aaattagttg
     6301 tgaaaatgtt aatggttgcg tgataatttc tatatttaaa ttagtttgaa gtgagggaga
     6361 gtatgtcgaa tcaaaattac gactacaata aaaatgaaga tggaagtaag aagaaaatga
     6421 gtacaacagc gaaagtagtt agcattgcga cggtattgct attactcgga ggattagtat
     6481 ttgcaatttt tgcatatgta gatcattcga ataaagctaa agaacgtatg ttgaacgaac
     6541 aaaagcagga acaaaaagaa aagcgtcaaa aagaaaatgc agaaaaagag agaaagaaaa
     6601 agcaacaaga ggaaaaagag cagaatgagc tagattcaca agcaaaccaa tatcagcaat
     6661 tgccacagca gaatcaatat caatatgtgc cacctcagca acaagcacct acaaagcaac
     6721 gtcctgctaa agaagagaat gatgataaag catcaaagga tgagtcgaaa gataaggatg
     6781 acaatgcatc tcaagataaa ccagatgata atcagaagaa aactgatgat aataaacaac
     6841 cagctcagcc taaaccacag ccgcaacaac caacaccaaa gccaaataat aatcaacaaa
     6901 ataatcaatc aaatcagcaa gcaaaaccac aagcaccaca acaaaatagc caatcaacaa
     6961 caaataaaca aaataatgct aatgataagt agtatttagt caaacaaaaa tgaaccagta
     7021 tgacagacaa cacaattaat taggttgtct cgaatattgg ttcttatttt tataattgtt
     7081 aattagggga gagatgatac ttaaatagtt agttgtttat tttacggaaa gtgaaattta
     7141 ttttgagtga ggtgggacag aaatgatatt ttcgcaaaat ttatttcgtc gtctgacccc
     7201 aacttccaca ttattgtaag ctgactttcc gtcagcttct gtgttggggc cccgccaact
     7261 tgcacattat tgtaagctga ctttccgcca gcttctgtgt tggggcccct gactttaatt
     7321 gaaaaatgct tgttacaagt gcattttcgt tcggtcacta ctactaatgt gacttttcgg
     7381 attctagagc attgatttat gtcttagttc aaataataag caaatgatta gcgagtcagt
     7441 ataagggtat acatttgact acagcgaata aaataaaacg ttttgttgaa taacaaatcg
     7501 aaaatatatt gcaagcgctt tatcaaatta tttagaaaat ttaagtttta tgcttgcaat
     7561 ttttgaaata gaatagtact attgcaagtg taaagaggtt aatttttgtc ccacgcggga
     7621 cttaaaaagg caaccactgg ttgtgacata tccttattta catttataaa tataaggagg
     7681 aggtagtagt gaaagactta ttgcaagcac agcaaaagct tataccggat ctcatagata
     7741 aaatgtataa acgtttttct attcttactg ctatctcaaa aaatcagcct gtcggacgtc
     7801 gaagtttaag cgaacatatg gatatgactg aacgtgtact gcgttctgaa acagatatgc
     7861 ttaagaaaca agatttgata aaagttaagc ctaccggaat ggaaattaca gctgaaggtg
     7921 agcaactgat ttcgcaattg aaaggttact ttgatatcta tgcagatgat aatcgtctgt
     7981 cagaaggtat taagaataaa tttcaaatta aggaagttca tgttgttcct ggtgatgctg
     8041 ataatagtca atctgttaaa acagaattag gtagacaagc aggtcaatta cttgaaggca
     8101 tattacaaga agacgcgata gttgctgtaa ctggcggatc cacgatggca tgtgttagtg
     8161 aagcaattca tttattacca tataatgtat tcttcgtacc agccagaggt ggactaggcg
     8221 aaaatgttgt ctttcaggca aacacaattg cagccagtat ggcacaacaa gctggcggtt
     8281 attatacgac gatgtatgta cctgataatg tcagtgaaac aacatataac acattgttgt
     8341 tagagccatc agtcataaac actttagaca aaattaaaca agcaaacgtt atattacacg
     8401 gcattggtga tgcgctgaag atggcgcatc gacgtcaatc acctgaaaag gtcattgaac
     8461 aacttcaaca tcatcaagct gtcggagagg catttggtta ttattttgat acacaaggtc
     8521 aaattgtcca taaggttaaa acaattggac ttcaattaga agaccttgaa tcaaaagact
     8581 ttatttttgc agttgcagga ggcaaatcga aaggtgaagc aattaaagca tacttgacga
     8641 ttgcacccaa gaatacagtg ttaatcactg atgaagccgc agcaaagata atacttgaat
     8701 aagagataaa aagtttaata ctttttaaat atcattttaa aggaggccat tataatggca
     8761 gtaaaagtag caattaatgg ttttggtaga attggtcgtt tagcattcag aagaattcaa
     8821 gaagtagaag gtcttgaagt tgtagcagta aacgacttaa cagatgacga catgttagcg
     8881 catttattaa aatatgacac tatgcaaggt cgtttcacag gtgaagtaga ggtagttgat
     8941 ggtggtttcc gcgtaaatgg taaagaagtt aaatcattca gtgaaccaga tgcaagcaaa
     9001 ttaccttgga aagacttaaa tatcgatgta gtgttagaat gtactggttt ctacactgat
     9061 aaagataaag cacaagctca tattgaagca ggcgctaaaa aagtattaat ctcagcacca
     9121 gctactggtg acttaaaaac aatcgtattc aacactaacc accaagagtt agacggttct
     9181 gaaacagttg tttcaggtgc ttcatgtact acaaactcat tagcaccagt tgctaaagtt
     9241 ttaaacgatg actttggttt agttgaaggt ttaatgacta caattcacgc ttacacaggt
     9301 gatcaaaata cacaagacgc acctcacaga aaaggtgaca aacgtcgtgc tcgtgcagcg
     9361 gcagaaaaca tcatccctaa ctcaacaggt gctgctaaag ctatcggtaa agttattcct
     9421 gaaatcgatg gtaaattaga tggtggtgca caacgtgttc ctgtagctac aggttcatta
     9481 actgaattaa cagtagtatt agaaaaacaa gacgtaacag ttgaacaagt taacgaagct
     9541 atgaaaaatg cttcaaacga atcattcggt tacactgaag acgaaatcgt ttcttcagac
     9601 gttgtaggta tgacttacgg ttcattattc gacgctacac aaactcgtgt aatgtcagtt
     9661 ggcgaccgtc aattagttaa agttgcagct tggtatgata acgaaatgtc atatactgca
     9721 caattagttc gtacattagc atacttagct gaactttcta aataatttta gtatagtttt
     9781 tattcaaata cgctagtgct cagaactatt tagcattaat taaagcttat gagtaagcgg
     9841 ggagcacaaa cgcttctccg cttattttta tataaaattt cctaattaca aggaggaaac
     9901 accatggcta aaaaaattgt ttctgattta gatcttaaag gtaaaacagt cctagtacgt
     9961 gctgatttta acgtaccttt aaaagacggt gaaattacta atgacaaccg tatcgttcaa
    10021 gctttaccta caattcaata catcatcgaa caaggtggta aaatcgtact attttcacat
    10081 ttaggtaaag tgaaagaaga aagtgataaa gcaaaattaa ctttacgtcc agttgctgaa
    10141 gacttatcta agaaattaga taaagaagtt gttttcgtac cagaaacacg cggcgaaaaa
    10201 cttgaagctg ctattaaaga ccttaaagaa ggcgacgtat tattagttga aaatacacgt
    10261 tatgaagatt tagacggtaa aaaagaatct aaaaatgatc cagaattagg taaatactgg
    10321 gcatctttag gtgatgtgtt tgtaaatgat gcttttggta ctgcgcatcg tgagcatgca
    10381 tctaatgttg gtatttctac acatttagaa actgcagctg gattcttaat ggataaagaa
    10441 attaagttta ttggtggcgt agttaacgat ccacataaac cagttgttgc tattttaggt
    10501 ggagcaaaag tatctgacaa aattaatgtc atcaaaaact tagttaacat agctgataaa
    10561 attatcatcg gcggaggtat ggcttatact ttcttaaaag cgcaaggtaa agaaattggt
    10621 atttcattat tagaagaaga taaaatcgac ttcgcaaaag atttattaga aaaacatggt
    10681 gataaaattg tattaccagt agacactaaa gttgctaaag aattttctaa tgatgccaaa
    10741 atcactgtag taccatctga ttcaattcca gcagaccaag aaggtatgga tattggacca
    10801 aacactgtaa aattatttgc agatgaatta gaaggtgcgc acactgttgt atggaatgga
    10861 cctatgggtg tattcgagtt cagtaacttt gcacaaggta caattggtgt atgtaaagca
    10921 attgcaaacc ttaaagatgc aattacgatt atcggtggcg gtgattcagc tgcagcagca
    10981 atctctttag gttttgaaaa tgacttcact catatttcaa ctggtggcgg cgcgtcatta
    11041 gagtacctag aaggtaaaga attgcctggt atcaaagcaa tcaataataa ataataaagt
    11101 gatagtttaa agtgatgtgg catgtttgtt taacattgtt acgggaaaac agtcacaaga
    11161 tgacatcgtg tttcatcact tttcaaaaat atttacaaaa caaggagtgt ctttaatgag
    11221 aacaccaatt atagctggta actggaaaat gaacaaaaca gtacaagaag caaaagactt
    11281 cgtcaatgca ttaccaacat taccagattc aaaagaagta gaatcagtaa tttgtgcacc
    11341 agcaattcaa ttagatgcat taactactgc agttaaagaa ggaaaagcac aaggtttaga
    11401 aatcggtgct caaaatacgt atttcgaaga taatggtgcg ttcacaggtg aaacgtctcc
    11461 agttgcatta gcagatttag gcgttaaata cgttgttatc ggtcattctg aacgtcgtga
    11521 attattccac gaaacagatg aagaaattaa caaaaaagcg cacgctattt tcaaacatgg
    11581 aatgactcca attatttgtg ttggtgaaac agacgaagag cgtgaaagtg gtaaagctaa
    11641 cgatgttgta ggtgagcaag ttaagaaagc tgttgcaggt ttatctgaag atcaacttaa
    11701 atcagttgta attgcttatg agccaatctg ggcaatcgga actggtaaat catcaacatc
    11761 tgaagatgca aatgaaatgt gtgcatttgt acgtcaaact attgctgact tatcaagcaa
    11821 agaagtatca gaagcaactc gtattcaata tggtggtagt gttaaaccta acaacattaa
    11881 agaatacatg gcacaaactg atattgatgg ggcattagta ggtggcgcat cacttaaagt
    11941 tgaagatttc gtacaattgt tagaaggtgc aaaataatca tggctaagaa accaactgcg
    12001 ttaattattt tagatggttt tgcgaaccgc gaaagcgaac atggtaatgc ggtaaaatta
    12061 gcaaacaagc ctaattttga tcgttattac aacaaatatc caacgactca aatcgaagcg
    12121 agtggcttag atgttggact acctgaagga caaatgggta actcagaagt tggtcatatg
    12181 aatatcggtg caggacgtat cgtttatcaa agtttaactc gaatcaataa atcaattgaa
    12241 gacggtgatt tctttgaaaa tgatgtttta aataatgcaa ttgcacacgt gaattcacat
    12301 gattcagcgt tacacatctt tggtttattg tctgacggtg gtgtgcacag tcattacaaa
    12361 catttatttg ctttgttaga acttgctaaa aaacaaggtg ttgaaaaagt ttacgtacac
    12421 gcatttttag atggtcgtga cgtagatcaa aaatccgctt tgaaatacat cgaagagact
    12481 gaagctaaat tcaatgaatt aggcattggt caatttgcat ctgtgtctgg tcgttattat
    12541 gcaatggacc gtgacaaacg ttgggaacgt gaagaaaaag cttacaatgc tattcgtaat
    12601 tttgatgccc caacttatgc aactgccaaa gaaggtgtag aagcaagcta taatgagggc
    12661 ttaactgacg aattcgtagt accattcatc gttgagaatc aaaatgacgg tgttaatgat
    12721 ggagatgcag tgatcttcta taatttccga cctgatagag cagcacaatt atcggaaatt
    12781 tttgcgaaca gagcattcga aggctttaaa gttgaacaag ttaaagactt attctatgca
    12841 acattcacta agtataacga caatatcgat gcggctatcg ttttcgaaaa agttgattta
    12901 aataatacaa ttggtgaaat tgcacaaaat aacaatttaa cacaattacg tattgcagaa
    12961 actgaaaaat atcctcacgt tacttacttt atgagtggtg gacgtaacga ggaatttaaa
    13021 ggtgaacgcc gtcgtttaat tgattcacct aaagttgcaa cgtatgactt gaaaccagaa
    13081 atgagtgctt atgaagttaa agatgcatta ttagaagagt taaataaagg tgacttggac
    13141 ttaattattt taaactttgc taaccctgat atggttggac atagtggtat gcttgagccg
    13201 acaatcaaag caatcgaagc ggttgatgaa tgtttaggtg aagtcgttga taagatttta
    13261 gacatggacg gttatgcaat tattactgct gaccatggta actctgatca agtattgacg
    13321 gatgatgatc aaccaatgac tacgcataca acgaacccag taccagtgat tgtaacaaaa
    13381 gaaggcgtta cacttcgaga aactggtcgc ttaggtgact tagcacctac attattagat
    13441 ttattaaatg tagaacaacc tgaagacatg acaggtgaat ctttaattaa acactaatat
    13501 tgtaaaagat gttaagtaaa cgcttaatga cacttatttt ttgaaaataa tagtaatatc
    13561 attttgttaa atgaaagaat aaagctataa taattataga ataactattt aaaggagatt
    13621 ataaacatgc caattattac agatgtttac gctcgcgaag tcttagactc tcgtggtaac
    13681 ccaactgttg aagtagaagt attaactgaa agtggcgcat ttggtcgtgc attagtacca
    13741 tcaggtgctt caactggtga acacgaagct gttgaattac gtgatggaga caaatcacgt
    13801 tatttaggta aaggtgttac taaagcagtt gaaaacgtta atgaaatcat cgcaccagaa
    13861 attattgaag gtgaattttc agtattagat caagtatcta ttgataaaat gatgatcgca
    13921 ttagacggta ctccaaacaa aggtaaatta ggtgcaaatg ctattttagg tgtatctatc
    13981 gcagtagcac gtgcagcagc tgacttatta ggtcaaccac tttacaaata tttaggtgga
    14041 tttaatggta agcagttacc agtaccaatg atgaacatcg ttaatggtgg ttctcactca
    14101 gatgctccaa ttgcattcca agaattcatg attttacctg taggtgctac aacgttcaaa
    14161 gaatcattac gttggggtac tgaaattttc cacaacttaa aatcaatttt aagcaaacgt
    14221 ggtttagaaa ctgcagtagg tgacgaaggt ggtttcgctc ctaaatttga aggtactgaa
    14281 gatgctgttg aaacaattat ccaagcaatc gaagcagctg gttacaaacc aggtgaagaa
    14341 gtattcttag gatttgactg tgcatcatca gaattctatg aaaatggtgt atatgactac
    14401 agtaagttcg aaggcgaaca cggtgcaaaa cgtacagctg cagaacaagt tgactactta
    14461 gaacaattag tagacaaata tcctatcatt acaattgaag acggtatgga cgaaaacgac
    14521 tgggatggtt ggaaacaact tacagaacgt atcggtgacc gtgtacaatt agtaggtgac
    14581 gatttattcg taacaaacac tgaaatttta gcaaaaggta ttgaaaacgg aattggtaac
    14641 tcaatcttaa ttaaagttaa ccaaatcggt acattaactg aaacatttga tgcaatcgaa
    14701 atggctcaaa aagctggtta cacagcagta gtttctcacc gttcaggtga aacagaagat
    14761 acaacaattg ctgatattgc tgttgctaca aacgctggtc aaattaaaac tggttcatta
    14821 tcacgtactg accgtattgc taaatacaat caattattac gtatcgaaga tgaattattt
    14881 gaaactgcta aatatgacgg tatcaaatca ttctataact tagataaata atcattttaa
    14941 acattaagta ctgccataat tttagttgag gattattatg tcggtataaa ttgaataaag
    15001 tttttgagtt cacgcttaaa aagttcacgc ttgtatttat aacctgccac agagttgaga
    15061 ctgtggtagg ttttttattt tgaagtatta ttcataacag actaataatc atgaggtaac
    15121 taataacaca tatttaactt gtattcttaa actggtataa taaatttatg ttgaaatgaa
    15181 tattgtatga cagggtattc acttttatta aaaggtaaaa ttaaataaag gttttataga
    15241 acgtatttaa atatatgagg agtaaacaaa tggctgatag aacgaataaa gaaattaaaa
    15301 caggacgctt tattgcaact gcatcaatcg tattctcaat attattgatt attcattact
    15361 ttgtttcgtt ggataatgcg actgccaaag cattacttaa tttaacgaat caaaacactt
    15421 cagataaagc gattgattac attttaaaca gctttagatt cactggtatt atgtatattt
    15481 tggcttatct agcaggcttc atcacttttt ggaatcgaca tacttatgtg tggtggttta
    15541 tgtttgcagt ttatgtatca aatagtttgt ttacgttgat taatttatca atcacaattc
    15601 aagcaataaa agctgcacac ggtgcgtact taacattgcc aattttaatc gttattatag
    15661 gttcggttgc attagcgatt tatatgcttg ttgtttctat caaacgtaaa agtacattta
    15721 atcgctagaa aattgatttt aacaataaaa atatgatata ctacttgtcg tatataagga
    15781 acggaggaca atttatgcat acatttttaa tcgtattatt aatcattgat tgtattgcat
    15841 taataactgt tgtactactc caagaaggta aaagcagtgg actttcaggt gccatcagtg
    15901 gtggtgctga gcagttattc ggtaaacaaa aacaacgtgg cgtcgattta ttcttaaata
    15961 gattaacaat tattttatca atattatttt ttgtacttat gatttgcata agttatcttg
    16021 gtatgtaagg tccggcgatg taaatgtcgg gcttttttat ttataattaa gaatgtaata
    16081 gtttaacaat aagctatgta aaatatatag cctagttaag tatgcaaagg gagcgttaga
    16141 tttatgcaga taaaattacc aaaacctttc ttttttgaag aaggcaaacg tgccgtgtta
    16201 ttattacatg gatttacagg caattcgtct gatgtacgtc aattaggtcg atttttacaa
    16261 aaaaagggat atacttcgta tgcaccacaa tatgaaggtc acgcggcacc accagaggaa
    16321 atactgaaat ctagtccttt cgtttggttt aaagatgcgt tagatggtta tgattatctt
    16381 gttgaacaag gttatgatga aattgttgtt gctggtctat cattaggtgg ggattttgct
    16441 ttaaaattaa gcttaaatag agatgtaaag ggtattgtaa cgatgtgtgc accaatgggt
    16501 ggcaaaactg aaggtgccat ttatgaaggc tttttagagt atgcacgtaa ttttaaaaag
    16561 tatgaaggta aaaatcaaga gactattgat aatgaaatgg atcattttaa accaactgaa
    16621 actttaaaag aactaagtga agcattagat acgattaaag atcaagttga tgaggtattt
    16681 gacccaattt tagtgattca agcagaaaac gacaatatga ttgatccaca atccgcaaat
    16741 tatatatatg accatgtaga ttctgatgac aaagacatca agtggtatag tcaatcagga
    16801 catgttatta cgattgataa agaaaaagaa caagtatttg aagatattta tcaattttta
    16861 gagtcattag actggtcaga ataaaaagag attttaacat tagaaaggag gggcataatg
    16921 aatttaaagc aatcaataga agagattatt aatcaacctg aatatgaacc tatgtcagtg
    16981 tcagattttc aagatgcatt aggtttaagt agtgccgact catttagaga tttaattaag
    17041 gtgctcgtag aattagaaca atcaggatta attgaacgta caaaaacaga cagatatcaa
    17101 aaaaagcata gttcaaaagg tcattcaaaa ttgataaagg gaacgttaag tcaaaataaa
    17161 aaaggctttg cattcttaag acctgaagat gaggatatgg aagatatatt tattccccca
    17221 acaaaaatta atcgtgcctt agatggagat actgtaattg tagaaataca tcaatctaaa
    17281 ggtgaacata aaggtaaaat tgaaggggaa gttaagtcga ttgagaagca ttcagtaact
    17341 caagttgttg gtacgtatag tgaagctaga catttcggat ttgttattcc agatgataaa
    17401 cgtattatgc aagatatttt cattcctaaa ggacaaagtt taggtgcagt agatgggcat
    17461 aaggtacttg ttcaaattac taaatatgct gatggttcag ataatccaga aggacatatt
    17521 tctgctattt taggacataa aaatgatcct ggcgtagata ttttatctat tatctatcaa
    17581 catggcatag aaattgaatt tcctgatgaa gtgttacaag aagctgaagc agtacctgat
    17641 catattgaaa atactgaaat taaaggccgt catgatttac gtgatgaatt gacaatcaca
    17701 attgatggtg ctgatgctaa agacttagat gacgcaatta gtgttaaaaa gttagcgaac
    17761 ggtaatacgc aattaactgt aagtattgct gatgtcagct attatgtaac agaaggttct
    17821 gcattggata aagaggcata tgatagagcg acaagtgtat atcttgttga ccgtgtaatt
    17881 ccaatgattc cacatcgatt aagtaatggt atttgttcat tgaatcctaa tgttgatcgt
    17941 ttaactctaa gctgtcgcat ggaaatcgat gctagtggtc gcgttgttaa acatgaaatt
    18001 tttgatagtg ttatacattc tgattatcga atgacgtatg atgcggtaaa tcagattatt
    18061 actgaaaagg atcctaacat tcgcgaacaa tataaagaaa ttacgcctat gttagattta
    18121 gcacaagatt tatctaatcg tttgattcaa atgagaaaac gacgtggtga aatcgatttt
    18181 gatattaatg aagcaaaagt attagttaac gaagacggta taccaacaga tgttcaatta
    18241 agacaacgtg gcgagggtga acgtctaatt gaatcattta tgttaattgc aaatgaaaca
    18301 gttgctgaac attttagtaa gttagatgta ccttttattt accgagtgca tgagcaacct
    18361 aaatcagatc gcttaagaca attctttgat tttattacaa actttggcat catgattaag
    18421 ggtactggcg aagatattca tccaacaaca cttcaaaagg ttcaagaaga agtagaaggt
    18481 cgacctgaac aaatggtcat ttcaacaatg atgttgcgtt caatgcaaca agcgcattat
    18541 gatgatgtga acttgggaca ttttggctta tcagctgaat attatacgca ttttacatca
    18601 ccaattagac gttatcctga tttaacagtt catcgtttaa tccgtaagta tttaattgag
    18661 aaatcaatgg ataacaaaga agtgaagcgt tgggaagaca aattgcctga gttagctgaa
    18721 catacttcta aacgtgaacg tcgtgctatt gaggcagaac gtgatactga tgaattgaaa
    18781 aaagcagaat atatgattca acatattggt gatgaatttg aaggtattgt cagctcagta
    18841 gctaacttcg gtatgttcat tgaattgcca aatacgatag aaggtatggt tcatattgcg
    18901 aatatgactg atgattatta ccgttttgaa gagcgtcaaa tggcattaat tggtgagcgt
    18961 caagctaaag tatttagaat tggtgacaca gttaaggtta aagtgacgca tgttgatgta
    19021 gatgaacgat taattgattt tcaaattgta ggtatgcctt taccgaaaaa tgatcgatca
    19081 cagcgcccag cgcgaggtaa gacaattcaa gccaaaacgc gtggtaaatc attagataaa
    19141 tcaaaatctg atgataaagg acgtaagaaa aagggtagtc aacgtaaagg taaaaatgaa
    19201 cgcaaaaatg acaaatcagg aaatagtaaa cataagccat tttataaaga taaaagtgtg
    19261 aaaaagaaag cacgtcgtaa gaaaaaataa gcagcaatga ggtgagtatg aatggctaag
    19321 aagaaatcac caggtacatt agcggaaaat cgtaaagcaa gacatgatta taatattgaa
    19381 gacacgattg aagcgggaat cgtattacaa ggtacagaaa taaaatcgat tcgccgaggt
    19441 agtgctaacc ttaaagatag ttacgcgcaa gttaaaaacg gtgaaatgta tttgaataat
    19501 atgcatatag caccatacga agaagggaat cgttttaatc acgatcctct tcgttctcga
    19561 aaattattat tgcataaacg tgaaatcatt aaattgggtg atcaaacacg tgaaattggt
    19621 tattcgattg tgccattaaa gctttatttg aagcatgggc attgtaaagt attacttggt
    19681 gtcgcacgag gtaagaaaaa atatgataaa cgtcaagctt tgaaagaaaa agcagtcaaa
    19741 cgagatgttg cgcgcgatat gaaagcccgt tattaagcga tttagttgct taatcgggct
    19801 atatttgata tagttatatg tgcttttgta aattacaaaa gtatgatttg tttgatttat
    19861 tatttcgggg acgttcatgg attcgacagg ggtcccccga gctcattaag cgtgtcggag
    19921 ggttgtcttc gtcatcaaca cacacagttt ataataactg gcaaatcaaa caataatttc
    19981 gcagtagctg cctaatcgca ctctgcatcg cctaacagca tttcctatat gctgttaacg
    20041 cgattcaacc ttaataggat atgctaaaca ctgccgtttg aagtctgttt agaagaaact
    20101 taatcaagct agcatcatgt tggttgttta tcacttttca tgatgcgaaa cctttcgata
    20161 aactacacac gtagaaagat gtgtatcagg acctctggac gcgggttcaa atcccgccgt
    20221 ctccatattt gtagcctaca acctttgtgg atgtgggctt ttttatatgt tttttatcta
    20281 ttcttgtgag gaaggttaaa ttagctgtgt acgttgatat gatagtattt gcttaggttc
    20341 aaattttcat gaatgaaaaa ttaatacttg tgtacatata agattttgcg ttaagggttg
    20401 aaagaatatg tgtcaaataa gtgtcaaaaa agttgagctt agagttttaa tattgttcat
    20461 a
//
LOCUS       DAMZKC010000033        20370 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.033, whole genome shotgun sequence.
ACCESSION   DAMZKC010000033 DAMZKC010000000
VERSION     DAMZKC010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 20370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20370
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.033"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            551..2800
                     /gene="pflB"
                     /locus_tag="POB55_000905"
     CDS             551..2800
                     /gene="pflB"
                     /locus_tag="POB55_000905"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDI6499879.1"
                     /translation="MLETNKNHATAWQGFKNGRWNRHVDVREFIQLNYTLYEGNDSFL
                     AGPTEATSKLWEQVMQLSKEERERGGMWDMDTKVASTITSHDAGYLDKDLETIVGVQT
                     EKPFKRSMQPFGGIRMAKAACEAYGYELDEETEKIFTDYRKTHNQGVFDAYSREMLNC
                     RKAGVITGLPDAYGRGRIIGDYRRVALYGVDFLMEEKMHDFNTMSTEMSEDVIRLREE
                     LSEQYRALKELKELGQKYGFDLSRPAENFKEAVQWLYLAYLAAIKEQNGAAMSLGRTS
                     TFLDIYAERDLKAGVITESEVQEIIDHFIMKLRIVKFARTPDYNELFSGDPTWVTESI
                     GGVGIDGRPLVTKNSFRFLHSLDNLGPAPEPNLTVLWSVRLPDNFKTYCAKMSIKTSS
                     IQYENDDIMRESYGDDYGIACCVSAMTIGKQMQFFGARANLAKTLLYAINGGKDEKSG
                     AQVGPNFEGINSEVLEYDEVFKKFDQMMDWLAGVYINSLNVIHYMHDKYSYERIEMAL
                     HDTEIVRTMATGIAGLSVAADSLSAIKYAQVKPIRNEEGLVVDFEIEGDFPKYGNNDD
                     RVDDIAVDLVERFMTKLRSHKTYRDSEHTMSVLTITSNVVYGKKTGNTPDGRKAGEPF
                     APGANPMHGRDQKGALSSLSSVAKIPYDCCKDGISNTFSIVPKSLGKEPEDQNRNLTS
                     MLDGYAMQCGHHLNINVFNRETLIDAMEHPEEYPQLTIRVSGYAVNFIKLTREQQLDV
                     ISRTFHESM"
     gene            2823..3578
                     /gene="pflA"
                     /locus_tag="POB55_000906"
     CDS             2823..3578
                     /gene="pflA"
                     /locus_tag="POB55_000906"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HDI6499880.1"
                     /translation="MLKGHLHSVESLGTVDGPGLRYILFTQGCLLRCLYCHNPDTWKI
                     SEPSREVTVDEMVNEILPYKPYFDASGGGVTVSGGEPLLQMPFLEKLFAELKENGVHT
                     CLDTSAGCANDTKAFQRHFEELQKHTDLILLDIKHIDNDKHIRLTGKPNTHILNFARK
                     LSDMKQPVWIRHVLVPGYSDDKDDLIKLGEFINSLDNVEKFEILPYHQLGVHKWKTLG
                     IAYELEDVEAPDDEAVKAAYRYVNFKGKIPVEL"
     gene            3914..5677
                     /locus_tag="POB55_000907"
     CDS             3914..5677
                     /locus_tag="POB55_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HDI6499881.1"
                     /translation="MKRISKDIWAVFKLLYQNKGRFSINALLLQLIMIFISSTYLILL
                     FNMMLKVAGQSQLTINNWTEIVSHPASVILLIIFILSVAFLIYVEFSLLVYMVYAGFD
                     RQIITFKSIFKNAFVNVRKLIGVPVIFFVIYLMLMIPIANLGLSSVLTKNIYIPKFLT
                     EELMKTTKGIIIYGTFMIAVFILNFKLIFTLPLTILNRQSLFKNMRLSWQITKRNKFR
                     LVIEIVILELIIGAILTLIISGATYLAICVDEEGDKFLVSSILFVVLKSALFFYYLFT
                     KLSLISVLVLHLKQENVLDQPGLEFKYPKPKRKSRFFIISMVLAVTCFIGYNMYLLYN
                     NTINTNISIIGHRGFEDKGVENSIPSLKAAAKANVEYVELDTIMTKDKQFVVSHDNNL
                     KRLTGVNKNISESNFKDVVGLKMRQNGHEAKLVSLDEFIETAKQSNVKLLVELKPHGK
                     EPADYTQRVIDILKKHGVEHQYRVMSLDYDVMTKLKKEAPYLKCGYIIPLQFGHFKET
                     SLDFFVIEDFSYSPRLVNQAHLENKEVYTWTINGEEDLTKYLQTNVDGIITDDPALAD
                     QIKEEKKDETYFDRSIRILFE"
     gene            complement(5841..6185)
                     /locus_tag="POB55_000908"
     CDS             complement(5841..6185)
                     /locus_tag="POB55_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement inhibitor SCIN family protein"
                     /protein_id="HDI6499882.1"
                     /translation="MTTQMKIKTYLVAGIKAALLDTTGIKLASKSETTSHTYQHQALV
                     DQLHELIANTDLNKLSYLNLDAFQKRDILAAHYIAKSAIRTKNLDQMTKAKHRLESIY
                     DSISNPLHSQNN"
     gene            6375..8276
                     /gene="coa"
                     /locus_tag="POB55_000909"
     CDS             6375..8276
                     /gene="coa"
                     /locus_tag="POB55_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009557200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylocoagulase"
                     /protein_id="HDI6499883.1"
                     /translation="MKKQIISLGALAVASSLFTWDNKADAIVTKDYSGKSQVNAGSKN
                     GKQIADGYYWGIIENLENQFYNIFHLLDQHKYAEKEYKDAVDKLKTRVLEEDQYLLER
                     KKEKYEIYKELYKKYKKENPNTQVKMKAFDKYDLGDLTMEEYNDLSKLLTKALDNFKL
                     EVKKIESENPDLKPYSESEERTAYGKIDSLVDQAYSVYFAYVTDAQHKTEALNLRAKI
                     DLILGDEKDPIRVTNQRTEKEMIKDLESIIDDFFIETKLNRPKHITRYDGTKHDYHKH
                     KDGFDALVKETREAVAKADESWKNKTVKKYEETVTKSPVVKEEKKVEEPQSPKFDNQQ
                     EVKITVDKAEETTQPVAQPLVKIPQGTITGEIVKGPEYPTMENKTLQGEIVQGPDFPT
                     MEQNRPSLSDNYTQPTTPNPILEGLEGSSSKLEIKPQGTESTLKGTQGESSDIEVKPQ
                     ASETTEASHYPARPQFNKTPKYVKYRDAGTGIREYNDGTFGYEARPRFNKPSETNAYN
                     VTTNQDGTVTYGARPTQNKPSKTNAYNVTTHANRQVSYGARPTQNKPSKTNAYNVTTH
                     ANGQVSYGARPTQNKPSKTNAYNVTTHANGQVSYGARPTYKKPSKTNAYNVTTHADGT
                     ATYGPRVTK"
     gene            complement(8458..8565)
                     /locus_tag="POB55_000910"
     CDS             complement(8458..8565)
                     /locus_tag="POB55_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499884.1"
                     /translation="MCVRTRLVSSSSARLSKAIIIAVIVVYHLDVRGLF"
     gene            complement(8879..10063)
                     /locus_tag="POB55_000911"
     CDS             complement(8879..10063)
                     /locus_tag="POB55_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HDI6499885.1"
                     /translation="MQEAYIVAYGRSAAAKAKQGALFHERPDDVAAKVLQGVLKRIDG
                     KFNKNMIEDVIVGTAFPEGLQGQNIARTIALLTGLSDTVPGQTVNRYCSSGLQTIAIA
                     ANQIMAGQGDILVAGGVELMSAVPMGGNEPTNNPTLQYDDIGASYPMGLTAENVASQF
                     DVSREDQDAYAVRSHQRAYEAQRDGRFNDEIIPIQVNSVEYTNAGPKVHTNIFDQDEF
                     IRPDTTMEALAKLRTVFKADGTVTAGTSAPLSDGAGFVVLMSGDKVKELGVTPIARFV
                     GYKAVGVDPKIMGIGPAYAIPEVLSLSNLSVEDIDLIELNEAFASQTIASIKEVGLDI
                     SRTNVNGGAIALGHPLGATGAMLTARLLNEMGRRPDSRYGMVTMCIGVGMGAAAIFEY
                     VR"
     gene            complement(10093..12354)
                     /locus_tag="POB55_000912"
     CDS             complement(10093..12354)
                     /locus_tag="POB55_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HDI6499886.1"
                     /translation="MTINKVTVLGAGTMGAQLAALFVNAGLKVKLLDIVVDKNDPNLI
                     AKKSYDKITDKKRPLLFDLNLASHLTYGNFDDDLVNDDADLYIEAVKEDIEIKHAVWQ
                     QVLQHAKEDALFATNTSGIPINAIAQTFNEKDQERFFGLHFFNPPRIMKLVELIPTSH
                     TKESIILDVKNFAQNVLGKGVIVVNDVPGFVANRVGTQTMNDIMYRAEQHKLSIVDVD
                     ALTGQAIGRPKTGTYALSDLVGLDIAVSVIKGMQQVPEETPYFHDVKIVNTLFDNGAL
                     GRKTKQGFYKKDKETKARLVYDVEKQDYVPVSQPQLPILNEFNKDLVHNLDVIFNAQD
                     EAGLFLWETLRNNFYYSAINVPKATDDFRDIDRALVWGFNWKLGPFQLWDAMGYERVK
                     TRMEDEIGDLPQWISDLDGGFYKQDETIEYATPVSHFVKDELWDKGDAKLSVTHDDQL
                     LLKLQSKNNVITDEFNDALVDAIDLLENDHYTSMVIYADGNNFSVGANLFLMKKAHED
                     GLVDDVVAQSIDKLHYSFNRLKYSLKPVVTAVQGRALGGGCELVLYSPIVVAASETYI
                     GLVEAGVGLLPSGGGLAEMADRILRTSHKFDDKQASMTKVLTNIAFAKVSTNAFEARR
                     YGYLRDTDTIIFNTAQRVEVALKRAKYEAETNYIPNPRHQYIALGEDFKALIQGQLDA
                     QRRGHFISDHDYHIALNIATILAGGDLPRNTFINQRYIQSLEKIGFIDLLKSKKSYER
                     IAHMLKTGKPLRN"
     gene            complement(12541..13752)
                     /locus_tag="POB55_000913"
     CDS             complement(12541..13752)
                     /locus_tag="POB55_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HDI6499887.1"
                     /translation="MTFEKETVLKTLFPEDVLSIAKGLTDGEVEFLQQVDSLLESKYR
                     ENINQHWIDATVPEDYFKDLGELNYFNNPLLYKDRPNAKMPSQLFQFFMSYLLARFDI
                     SLATLLGVHQGLGHNTFYFGGSKEQIAKYVPKLQSHELRTCFALTEPEHGSDVAGGLE
                     TVAERQGDTWVINGEKKWIGGAHVSDVIPVFAVNKETGKPHCFVVRPEQDGVDIEVID
                     NKIALRIVPNALIKLTNVKVDEADRLQNITSFKDIAKILYSTRAGVAYMATGGMAGAL
                     RATLDYVTERKQFGKPISKYQLIQEKLAMMQGNLAQAMATCAQLANMQAHGEYDEVAT
                     STAKMMNALRLRETVAMGRGITGGNGILADDYDIARFFSDAEAIYTYEGTHEINALVI
                     GRALTGDSAFV"
     gene            complement(13864..15369)
                     /locus_tag="POB55_000914"
     CDS             complement(13864..15369)
                     /locus_tag="POB55_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl--CoA ligase"
                     /protein_id="HDI6499888.1"
                     /translation="MNFDWIKTRSDFDDDKPAVIDHAKQTSWTYQQLNARADNMAHYL
                     TSQGVKKGDVIGIFAPNDIAILDLLFACFKTGAVFLPLNWRLNPKEIAAIVEDAQLKL
                     LFYAEKHLSSLTDIDQNLLHMDIDVAQYDEIVNPDYHQPFQATPVEPQDLAALIYTSG
                     TTGSPKGVMFSYESFVHNGANLELTYKFNSNYITIVSTPMFHVLGFNDTVLPVLMSGG
                     TLILQRYFNGEELNDMIAQYHPTFIIMIPTMYYSTLRASNFNPENFKAMDYIIQGGSQ
                     PLPSIQAAFKQYGINIINGYGLTEAPLVLVNTPENSKRKPMSIGKAVMFVDARILDDN
                     GEEVPTGEIGELAIKAKNVTPGYWNKPAETAKAFHGRYLLTGDLAKMDDDGDIFIIER
                     KKELIITGGENVLPSEVENALAEHPLVDRCVVVGYDHPKYGESIAAAIILREDEPHYA
                     EILDQHMRSRLAGYKVPRMYVPVTHMPLNSTQKPDKLAIRQMMNDKVSQTL"
     gene            complement(15395..16972)
                     /locus_tag="POB55_000915"
     CDS             complement(15395..16972)
                     /locus_tag="POB55_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HDI6499889.1"
                     /translation="MEVTCLKQITWHDLQHIIKDGDVIGLPALAVANLPAEVLRAVLA
                     QHDTYHTPKDLTFILANDIHSLGAAPDLDDFIERRMIKRVIMSILTASSKTAQAMKNN
                     DIEAYFLPQGIIATHYRQSNQLLPGVITKIGLNTAVDPRYGGGKVNTRTTDDLVSLVT
                     INDETYLHYTFPSVDVALLRGTYADQQGNIYLTQEAYLSECYHVALNAKANHGKVIVQ
                     VKALVDGYQLKPNEVVIPGNLVDYVYVTEDEKNHRQVIQSHYLPALSGEERIDGIPEP
                     ALPFNSRKLILRRAAQFLTYGDTISIGYGINNELSNLLHEECVEHDVQPILDVGIFGG
                     FVGSREHFGMNYNADVRMPHDRAWDFIYNNGVSVAYLSFAEVDQYGNVNVSYFNDRLN
                     GCGGFIDITQSVNKIIFSGTFVAGSHVSCHNQRLNIETEGQNQKFVSDVSHIDFNAQY
                     SQSLEQEVYFVTERAVFELTNQGLKLIEIAPGLDLHKDILNQMAFKPIIADHLKLIDT
                     SIYKEKWGQLKQSIHKV"
     gene            17413..18555
                     /locus_tag="POB55_000916"
     CDS             17413..18555
                     /locus_tag="POB55_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HDI6499890.1"
                     /translation="MSSASTQSTKTSDIHNESINKQMEAKAHETAQNADLKTEARSLF
                     DNATKSIGRLAGNDESLNLNLKDMFSEVFKPHTKNEADEIFIAGTAKTTPAICDISEE
                     WGKPWLFSRVFIAFTVTFIGLWVMAAIFNNTNAIPGLIFIGALTVPLSGLFFFYESNA
                     FKNISIFEVIIMFFIGGVFSLLSTMLLYRFVVFSDQFERFGSLTFFDAFLVGLVEETG
                     KALIIVYFVNKLKTNKILNGLLIGAAIGAGFAVFESAGYILNFALGENVPLLDIVFTR
                     AWTAIGGHLVWSAIVGAAIVIAKEQHGFEFKDIFDKRFLIFFLSAVGLHGIWDTSLTV
                     LGSDTLKIFILIVIVWILVFILMGAGLKQVNLLQKEFKEQQKKVDE"
     gene            complement(18869..20344)
                     /locus_tag="POB55_000917"
     CDS             complement(18869..20344)
                     /locus_tag="POB55_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDI6499891.1"
                     /translation="MKFKRLATIFSAVLVLSGCGSMHSSGKDLNISLPLKTKSIAPYE
                     TDVPVKIGAAESLFKTNDQGKIEKALVKSYHQPNDTTLDIELKDNIKFQNGQKLTAEK
                     VKSSLENSMKKSDLVKYSLPISSITAKGQKLTIKTNSAYPELVSELANPFMAIYDTDA
                     KSDVNQTPVGTGPYQIKDYKQSRKISLSNFKDYWQGKPKLDHITVTYQEDGNNRVRNL
                     ESQKDDLITDVPVNKVQDIENNQNLKVSKESGFRTSLLMYNHTNKKMTKSVREALDHI
                     IDRQGIADHIYQGYAKPATSPFNDKIPYIKEPKLTKQNIEQAKTLLAKDGYTKEHPLK
                     IKLITYDGRPELSKIAQVLQSDAKKANIEIDIKSVDDIEGYLKDRSAWDATMYSFGTI
                     PRGDTGYFFNQAYKKDGAINKGDYNNSNVDDLINQLNHTVDVKERHNISNDIIKLSSR
                     DVPNSYIAYNDQIVAANAKVKNYKVTPEGIYLIDYRTTIER"
BASE COUNT         6733 a         3532 c         3577 g         6528 t
ORIGIN      
        1 attttagcaa taaatgacgc ataagtaatg ttaaatattt agaaatgttt aaagatgact
       61 tgttaagacg ttgcaaatgt tgtgatagca cataattttt gtttgtcaag atgacttacc
      121 gaggttgtaa aatcaaactg ttatatttta tttgtagctg ttatataaaa attggcaaga
      181 tattgaacgg ttcaaaagta tatttttacg tcaataaaag tatttaatcc agtctcttca
      241 tatataaaag taaatctttc taagtgttga tttaacgctt atcaacaatc attttttata
      301 aacaaatata tactcctaaa ttaactttta aagcaatgaa aatagtgaac attataactg
      361 ttgtgtaaca gaatgcaatt agcatattac tgttacacaa attagtacag tttctatgtt
      421 ttgacataca tttgatgaaa attgtacata atttatgtga aaaaaatcac aacaaacatg
      481 ctacaatgac tatgaaaacg ttaacatagc atttcaaatt cacaacatta tacagatgga
      541 ggcgtttagt atgttagaaa caaataaaaa tcatgcaaca gcttggcaag gatttaaaaa
      601 tggaagatgg aacagacacg tagatgtaag agagtttatc caattaaact acactcttta
      661 tgaaggtaat gattcatttt tagcaggacc aacagaagca acttctaaac tttgggaaca
      721 agtaatgcag ttatcgaaag aagaacgtga acgtggcggc atgtgggata tggacacgaa
      781 agtagcttca acaatcacat ctcatgatgc tggttattta gacaaagatt tagaaacaat
      841 tgtaggtgta caaactgaaa agccattcaa acgttcaatg caaccattcg gtggtattcg
      901 tatggcgaaa gcagcttgtg aagcttacgg ttacgaatta gacgaagaaa ctgaaaaaat
      961 ctttacagat tatcgtaaaa cacataacca aggtgtattc gatgcatatt ctagagaaat
     1021 gttgaactgc cgtaaagcag gtgtaatcac tggtttacct gatgcatacg gacgtggacg
     1081 tattatcggt gactatcgtc gtgtagcttt atatggtgta gatttcttaa tggaagaaaa
     1141 aatgcacgac ttcaacacga tgtctacaga aatgtcagaa gatgtaattc gtttacgtga
     1201 agaattatca gaacaatatc gtgcattaaa agaattaaaa gaacttggac aaaaatatgg
     1261 tttcgattta agccgtccag cagaaaactt caaagaagca gttcaatggt tatacttagc
     1321 ataccttgct gcaattaaag aacaaaacgg tgcagcaatg agtttaggtc gtacatcaac
     1381 attcttagat atctatgctg aacgtgacct taaagcaggc gttattactg aaagcgaagt
     1441 tcaagaaatt attgaccact tcatcatgaa attacgtatt gttaaatttg ctcgtacacc
     1501 tgattacaat gaattattct ctggagaccc aacttgggta actgaatcta tcggtggtgt
     1561 aggtattgac ggacgtccac ttgttacgaa aaactcattc cgtttcttac actcattaga
     1621 taacttaggt ccagctccag aaccaaactt aacagtatta tggtcagtac gtttacctga
     1681 caacttcaaa acatactgtg caaaaatgag tattaaaaca agttctatcc aatatgaaaa
     1741 tgatgacatt atgcgtgaaa gctatggcga tgactatggt atcgcatgtt gtgtatcagc
     1801 gatgacaatt ggtaaacaaa tgcaattctt cggtgcacgt gcgaacttag ctaaaacatt
     1861 actttacgct atcaatggtg gtaaagatga aaaatctggt gcacaagttg gtccaaactt
     1921 cgaaggtatt aacagcgaag tattagaata tgacgaagta ttcaagaaat ttgatcaaat
     1981 gatggattgg ctagcaggtg tttacattaa ctcattaaat gttattcact acatgcacga
     2041 taaatacagc tatgaacgta ttgaaatggc attacatgat acagaaattg tacgtacaat
     2101 ggcaacaggt atcgctggtt tatcagtagc agctgactca ttatctgcaa ttaaatatgc
     2161 acaagttaaa ccaattcgta acgaagaagg tcttgtagta gactttgaaa tcgaaggcga
     2221 cttccctaaa tacggtaaca atgacgaccg tgtagatgat atcgcagttg atttagtaga
     2281 acgctttatg actaaattac gtagtcataa aacatatcgt gattcagaac atacaatgag
     2341 tgtattaaca attacttcaa acgttgtata cggtaagaaa actggtaaca caccagacgg
     2401 acgtaaagct ggcgaaccat ttgcaccagg tgcaaaccca atgcatggcc gtgaccaaaa
     2461 aggtgcatta tcttcattaa gttctgtagc taagatccct tacgattgct gtaaagatgg
     2521 tatttcaaat acattcagta tcgtaccaaa atcattaggt aaagaaccag aagatcaaaa
     2581 ccgtaactta actagtatgt tagatggtta cgcaatgcaa tgtggtcacc acttaaatat
     2641 taacgtattt aaccgtgaaa cattaataga tgcaatggaa catccagaag aatatccaca
     2701 gttaacaatc cgtgtatctg gttacgctgt taacttcatt aaattaacac gtgaacaaca
     2761 attagatgta atttctcgta cattccatga aagtatgtaa caaaatttaa ggtgggagca
     2821 ctatgcttaa gggacactta cattctgtcg aaagtttagg tactgtcgat ggaccgggat
     2881 taagatatat attatttaca caaggatgct tacttagatg cttgtattgc cacaatccag
     2941 atacttggaa aattagtgag ccatcaagag aagtcacagt tgatgaaatg gtgaatgaaa
     3001 tattaccata caaaccatac tttgatgcat cgggtggcgg tgtaacagtc agtggtggcg
     3061 aaccattgtt acaaatgcca ttcttagaaa aattatttgc ggaattaaaa gaaaatggtg
     3121 tgcacacttg cttagacaca tcggctggat gtgctaatga tacaaaagca tttcaaaggc
     3181 attttgaaga attacaaaaa catacagact tgatattatt agatataaaa catattgata
     3241 atgacaaaca tattagattg acaggaaagc ctaatacaca catccttaac ttcgcgcgca
     3301 aactgtcaga tatgaaacaa cctgtatgga ttcgacatgt ccttgtgcct ggttattctg
     3361 atgataaaga cgatttaatt aaactagggg aattcattaa ttctcttgat aacgtcgaaa
     3421 agtttgaaat tctgccatat catcagttag gtgttcataa gtggaaaaca ttgggcattg
     3481 catatgaatt agaagatgtc gaagcgcccg atgatgaagc tgttaaagca gcctaccgtt
     3541 atgttaactt caaagggaaa attcccgttg aattataaat acaattcaga ccgaaaagaa
     3601 agcatatgca acttcaagag tgaaggggca tatgcttctt tttcaattga gtatagagta
     3661 ttagcaagac gtagtaagta tatgagacaa cttctacaat ggttgaagga agacgttttt
     3721 gtaagtagct atgctgataa agaatgtgat gtcttgttaa aggtggggtt ccaatatcat
     3781 catttagctg atgttgaatg ggttattatt tgctacttgc atatgaatat gagtcttttc
     3841 aaatttttat tgaccctgag taatgaaaaa tattaagatg aaacttaata ttaaagcaat
     3901 gcggagcgtg attatgaaga gaattagtaa agatatatgg gcagtattta aattactgta
     3961 tcaaaataaa gggcgtttta gcattaatgc cttactattg cagttaatca tgatttttat
     4021 tagtagtaca tacttaattt tactatttaa tatgatgtta aaagtagctg ggcaaagcca
     4081 acttacgatt aacaattgga cggaaatcgt tagtcatccc gccagtgtga tacttcttat
     4141 tatattcata ttaagtgttg cctttctgat ttatgtagag ttttcattgt tagtttatat
     4201 ggtttatgcc ggctttgatc gacagattat tacatttaaa tccattttta aaaatgcctt
     4261 tgtaaatgtg cgtaaactca taggtgtacc agttattttc tttgttattt atttaatgtt
     4321 aatgataccc attgccaacc taggactaag ttcagtatta acaaaaaata tttacatacc
     4381 taaattttta acggaagaac ttatgaaaac gacgaaaggt ataatcattt acggtacctt
     4441 tatgattgct gtatttatat taaactttaa attaatattt accttaccgt taacgatttt
     4501 aaaccgtcag tcgttattta aaaatatgag actaagttgg caaattacga agcgaaataa
     4561 gtttcgactt gttatagaaa tagttatatt ggaactcatc attggtgcga ttttaacatt
     4621 aattatttca ggagcaacat atcttgccat ttgtgtagat gaagaaggag ataagttttt
     4681 agtctcatca attttatttg ttgtattgaa aagcgcattg ttcttctatt atttatttac
     4741 gaaattatca ttaatcagtg tgttagtact gcacttaaaa caagagaatg tattagacca
     4801 accgggctta gaatttaaat acccaaaacc gaaacggaag tctaggttct ttataatttc
     4861 aatggtactt gcagtgacat gttttatcgg ttataacatg tacttacttt acaataatac
     4921 tatcaataca aatatctcca ttattggcca tcgtggtttc gaagataaag gcgttgaaaa
     4981 ttctattccg tcattgaaag ctgctgcaaa agcgaatgtc gaatacgttg agttagatac
     5041 aattatgacg aaagataaac aatttgttgt tagtcatgat aacaatttga aacgtttaac
     5101 aggtgttaat aaaaatattt ctgaatctaa tttcaaagat gtcgtcggtt tgaaaatgcg
     5161 tcaaaatgga catgaagcaa aacttgtatc cttagacgaa tttattgaaa cggctaaaca
     5221 atcaaatgtg aagctactag tagagttaaa gccacatggt aaagaaccag cagattatac
     5281 acaacgtgtt attgatattt tgaaaaagca tggtgttgaa catcaatatc gtgtgatgtc
     5341 attggattat gatgtgatga ctaagttgaa aaaagaagcg ccatatctca agtgtggtta
     5401 tatcattccg ttgcagtttg gtcattttaa agaaacatca ttagatttct ttgtcatcga
     5461 agatttttct tattcgccaa gacttgttaa tcaagcgcac ttggaaaata aagaagtcta
     5521 tacttggacc attaacggcg aagaagattt aacgaaatac ttacaaacca atgttgatgg
     5581 tattatcaca gatgacccag cattagctga tcagattaaa gaagaaaaga aagacgaaac
     5641 atacttcgat cgttctataa gaattttgtt tgaataatat aaacaaagac ctctaaagtt
     5701 atcaagatga taccttcaga ggtcttttta atgttgccat ctatgggata ggcaatcgtt
     5761 tcattcgttt atattcatat gacaagtatt tgtatggcaa tttggcgtca caaacactta
     5821 catgatttat tgataagttg ctaattgttt tgtgaatgca aagggttaga aattgaatcg
     5881 taaatacttt ctaatctatg tttcgcttta gtcatttgat ccaaattttt agtgcgtata
     5941 gcggattttg caatatagtg cgcagctaaa atatcgcgtt tttgaaacgc atctaaattt
     6001 aggtacgata atttatttaa gtcagtgttt gctattaatt catgtaattg atctacaagc
     6061 gcttgatgtt gatacgtatg tgatgtagtt tcagatttgc ttgctaattt aataccagtc
     6121 gtatcaagga gcgccgcttt aataccagca actaaatatg ttttgatttt catttgtgtt
     6181 gtcatgcttt gttactcctt tgatgtacat taatcaaaaa aattatacac tattgtatat
     6241 tgcaaagcta attaactata acaaaaagat agttaatgct ttgtttattc tagttaatat
     6301 atagttaatg tcttttaata ttttgtttct ttaatgtaga ttgggcaatt acattttgga
     6361 ggaattaaaa aattatgaaa aagcaaataa tttcgctagg cgcattagca gttgcatcta
     6421 gcttatttac atgggataac aaagcagatg cgatagtaac aaaggattat agtgggaaat
     6481 cacaagttaa tgctgggagt aaaaatggga aacaaattgc agatggatat tattggggaa
     6541 taattgaaaa tctagaaaac cagttttaca atatttttca tttactggat cagcataaat
     6601 atgcagaaaa agaatataaa gatgcagtag ataaattaaa aactagagtt ttagaggaag
     6661 accaatacct gctagaaaga aaaaaagaaa aatacgaaat ttataaagaa ctatataaaa
     6721 aatacaaaaa agagaatcct aatactcaag ttaaaatgaa agcatttgat aaatacgatc
     6781 ttggcgattt aactatggaa gaatacaatg acttatcaaa attattaaca aaagcattgg
     6841 ataactttaa gttagaagta aagaaaattg aatcagagaa tccagattta aaaccatatt
     6901 ctgaaagcga agaaagaaca gcatatggta aaatagattc acttgttgat caagcatata
     6961 gtgtatattt tgcctacgtt acagatgcac aacataaaac agaagcatta aatcttaggg
     7021 cgaaaattga tttgatttta ggtgatgaaa aagatccaat tagagttacg aatcaacgta
     7081 ctgaaaaaga aatgattaaa gatttagaat ctattattga tgatttcttc attgaaacca
     7141 agttgaatag acctaaacac attactaggt atgatggaac taaacatgat taccataaac
     7201 ataaagatgg atttgatgct ctagttaaag aaacaagaga agcggttgca aaggctgacg
     7261 aatcttggaa aaataaaact gtcaaaaaat acgaggaaac tgtaacaaaa tctccagttg
     7321 taaaagaaga gaagaaagtt gaagaacctc aatcacctaa atttgataac caacaagagg
     7381 ttaaaattac agttgataaa gctgaagaaa caacacaacc agtggcacag ccattagtta
     7441 aaattccaca gggcacaatt acaggtgaaa ttgtaaaagg tccggaatat ccaacgatgg
     7501 aaaataaaac gttacaaggt gaaatcgttc aaggtccaga tttcccaaca atggaacaaa
     7561 acagaccatc tttaagcgat aattatactc aaccgacgac accgaaccct attttagaag
     7621 gtcttgaagg tagctcatct aaacttgaaa taaaaccaca aggtactgaa tcaacgttaa
     7681 aaggtactca aggagaatca agtgatattg aagttaaacc tcaagcatct gaaacaacag
     7741 aagcatcaca ttatccagca agacctcaat ttaacaaaac acctaaatat gttaaatata
     7801 gagatgctgg tacaggtatc cgtgaataca acgatggaac atttggatat gaagcgagac
     7861 caagattcaa taagccatca gaaacaaacg catacaacgt aacgacaaat caagatggca
     7921 cagtaacata tggcgctcgc ccaacacaaa acaaaccaag caaaacaaat gcatacaacg
     7981 taacaacaca tgcaaatcgt caagtatcat atggcgctcg cccgacacaa aacaagccaa
     8041 gcaaaacaaa tgcatataac gtaacaacac atgcaaatgg tcaagtatca tacggagctc
     8101 gcccgacaca aaacaagcca agcaaaacaa atgcatataa cgtaacaaca cacgcaaacg
     8161 gtcaagtgtc atacggagct cgcccgacat acaagaagcc aagtaaaaca aatgcataca
     8221 atgtaacaac acatgcagat ggtactgcga catatgggcc tagagtaaca aaataagttt
     8281 gtaactctat ccaaagacat acagtcaata caaaacatta cgtatcttta caacagtaat
     8341 catgcattct atgatgcttc taactgaatt aaagcatcga acaatcggaa gcatatttct
     8401 aaattattta ttcattatag tcttaaacat aacatgacct aatatattac taacctatta
     8461 aaataaacca cgcacatcta agtgatatac gacaatcaca gcaataataa ttgctttaga
     8521 aagtcgtgcc gaactggaac ttacaagtct agttcgaaca cacactgatg tgagtggttt
     8581 tctttatttt aaacatgaac aatcagataa gttactagca ttagcaaata ttattaaatc
     8641 aaagggcttc gattcataaa atttaaaaca atgattaaaa ttagacgtgt aaatgttaaa
     8701 ttctaaaacg gaaataacca ccatcccatt aaaccacttt tttgttcaat cactatattt
     8761 cacacagctt cattaataaa acgaaattgc ttcaacccgc ttcaacttca actggcttca
     8821 acttcagcct acttcattca ataacaaaac gaatccgctt catccaaaat caaccattct
     8881 aacgcacata ttcaaatata gcagctgcac ccataccgac accaatacac atcgtaacca
     8941 tgccgtaacg gctatcggga cgtctaccca tttcattaag taaacgcgcg gttaacattg
     9001 cgcctgtagc acctaatgga tgacctaaag caatagcgcc accattcaca ttcgtacgtg
     9061 atatatctag acctacttct ttaatagatg caattgtttg agaagcaaat gcttcgttca
     9121 attcgatcaa atcaatgtct tcaacagata gattgctgag tgacaatact tcaggaatcg
     9181 catatgcagg cccaataccc ataattttcg ggtcaacgcc tactgcctta taaccaacga
     9241 atcgtgcaat aggtgtcacg cctagttctt tcactttatc tccagacatt aaaactacaa
     9301 atcctgcacc atcagaaagt ggggcagatg ttcccgcagt cacagtgccg tcagctttaa
     9361 atactgtacg taatttggct aatgcctcca tcgtggtgtc agggcgtata aattcatctt
     9421 ggtcaaagat atttgtgtgt acttttggtc ctgcgtttgt atattcaact gagtttactt
     9481 gtattgggat aatttcatcg ttgaaccgac catcacgttg tgcttcatag gcacgttgat
     9541 gacttctgac agcataagca tcttgatctt cgcgtgatac gtcaaattgg gatgctacat
     9601 tttcagcagt taaacccata ggatatgacg cacctatatc atcatattgt aaggtaggat
     9661 tgtttgtggg ctcgttgcca cccattggta cggcactcat caattcaacg ccaccagcta
     9721 caagtatatc tccttgacca gccataattt gattggctgc aatcgcgatg gtttgtaatc
     9781 ctgatgagca gtagcgattc actgtttgac ccggtaccgt gtcagataat cccgtaagca
     9841 atgcaatcgt tcgtgcaatg ttttgtcctt gtaatccttc tggaaaagcc gtaccaacaa
     9901 tgacatcttc aatcatattc ttattgaatt ttccgtcaat acgtttcaat acgccttgta
     9961 atactttggc tgcgacatca tcaggtcttt cgtggaataa tgcgccttgc tttgctttcg
    10021 ctgcggctga acgcccataa gctacaatgt atgcttcttg catggttatc atcctctctt
    10081 aatgactatc ttttaattac gtaatggctt accagttttt aacatatgtg caattctttc
    10141 atatgatttt ttagatttta gtaagtcaat aaagccaatt ttctccaacg attgaatgta
    10201 acgttgattg ataaatgtat ttcttggtaa atcaccaccc gctaaaattg tggcgatatt
    10261 taaggcgata tgataatcat ggtcgctaat aaaatgaccc cgtctttgcg catctaattg
    10321 tccttggatc aatgctttga agtcttcacc taaagcgata tattgatgtc taggattcgg
    10381 aatatagttt gtttctgctt catatttcgc acgtttgagc gcaacttcga cacgttgtgc
    10441 tgtattgaaa ataatcgtat ctgtatcacg taaataacca taacgacgtg cctcaaaggc
    10501 atttgtagag actttcgcaa atgcgatatt cgtcagtact tttgtcatgg aagcttgttt
    10561 gtcatcaaac ttatgcgatg tgcgtaatat gcgatcagcc atttctgcaa ggccaccgcc
    10621 actcggtaat aagccaacgc ctgcttcaac aagaccgata tacgtttcac ttgcagcgac
    10681 aacaataggt gagtaaagta caagctcaca gccaccgcct aaggcacgac cttgaacagc
    10741 tgtgactact ggtttcaaac tatacttcaa acgattaaag ctataatgta atttatcaat
    10801 tgattgtgca acgacatcat ctacaagacc gtcttcatgc gcctttttca ttaagaaaag
    10861 gttagcaccc acactgaaat tgttaccatc tgcataaata accatacttg tgtaatggtc
    10921 attttccagt aaatcaatcg catcaactaa cgcatcgttg aattcatcgg taatgacatt
    10981 atttttactt tgtaatttca gtaacagttg atcatcatga gttacggaaa gtttggcatc
    11041 acctttatcc caaagttcat cttttacgaa gtgagaaaca ggtgttgcat attcgatggt
    11101 ctcatcttgt ttataaaagc caccatctaa atcactaatc cattgtggta agtctccaat
    11161 ttcgtcttcc atacgtgttt taacacgttc atatcccatt gcatcccata attggaatgg
    11221 accaagtttc cagttgaacc cccagacaag cgcacggtct atgtctcgga aatcatcggt
    11281 agctttaggt acattgatag cagagtaata gaaattatta cgtaatgtct cccataaaaa
    11341 tagtcccgct tcgtcttgcg cattgaatat gacatcaagg ttatgcacta agtctttatt
    11401 aaattcattt aaaattggta attgtggttg cgatacaggt acataatctt gtttttcaac
    11461 atcgtaaaca agtcgagctt tagtttcttt atcctttttg taaaatcctt gtttcgtttt
    11521 acgtccgagt gcgccattgt caaacaacgt atttacaatt ttgacatcat gaaaataagg
    11581 cgtttcttca ggtacttgtt gcatgccttt aattacagac actgcaatat ctaaaccgac
    11641 taggtcagat agggcatatg tacctgtttt aggacgacca atcgcttgcc cagttaaagc
    11701 atccacatct acaatgctta acttgtgttg ctcggcgcga tacataatat cattcattgt
    11761 ttgcgtgccg actctatttg cgacaaagcc aggcacatca ttgacgacaa tgacaccttt
    11821 acctaacaca ttttgcgcga aattttttac atctaatatg atagattcct tcgtgtgtga
    11881 cgtaggtatt aactccacta atttcataat acgtggtggg ttaaagaaat gtagaccaaa
    11941 gaatcgttct tgatccttct cgttaaatgt ttgagcaatc gcattaattg gaatacctga
    12001 tgtatttgta gcgaataaag catcttcttt agcatgttgt agaacttgtt gccaaacagc
    12061 atgcttaatt tcaatatctt ctttaactgc ttcgatatat aaatcagcat catcatttac
    12121 caagtcatca tcaaaattac catatgttaa atgactcgct agatttaagt cgaatagtag
    12181 cggccgtttc ttatctgtaa ttttatcgta agattttttc gcaatgagat ttggatcgtt
    12241 tttgtccact acaatatcta atagttttac tttaagtcca gcattcacaa agagtgctgc
    12301 cagttgagcg cccatcgtgc ctgcgccaag aacggttact ttattaattg tcatagtgat
    12361 tcctccaatt tagttgagga taagataacc attaagataa ttggaataac gttgctattt
    12421 tataaaatta attaagtatc tttgacagtc atcttagcct cttatttaag gaaaaagctt
    12481 tatgcttaaa ataagtcttt ttttagtgat attactgcat ctcatataat tctttgctat
    12541 ttatacgaaa gcagaatctc cagtcaaagc gcgtccaatt actaaggcat taatttcatg
    12601 tgtaccttcg tacgtgtaaa tcgcttctgc atcagagaag aaacgtgcaa tatcataatc
    12661 gtcagctagt atgccattac cacctgtaat accacggccc atagctactg tctcacgcaa
    12721 acgtaaggca ttcatcatct tcgccgttga agttgcaacc tcgtcatatt caccatgtgc
    12781 ttgcatatta gctaattgag cacatgttgc cattgcttga gctaaattac cttgcatcat
    12841 tgctagcttt tcttgtatta actgatattt actaattggt ttgccgaatt gcttacgctc
    12901 agtgacataa tctaatgtgg cacgtaaagc gccagccata ccacctgtag ccatataagc
    12961 aacgcctgct ctcgttgaat aaagaatttt ggcaatatct ttaaagcttg ttatgttttg
    13021 taagcgatcc gcttcatcta ctttgacatt agttaattta attagggcgt taggaacaat
    13081 gcgaagtgcg attttattat caatgacttc aatatcgacg ccatcttgtt ctggtctgac
    13141 tacaaagcaa tggggtttgc cagtttcttt atttactgcg aatactggaa tgacatcaga
    13201 tacatgtgca ccaccaatcc atttcttttc accattgata acccaagtat cgccctggcg
    13261 ttcagcgact gtttcaagac ctcccgcaac gtccgaaccg tgttctggtt cagttaaagc
    13321 aaagcatgta cgcagttcat gtgactgtaa tttaggtaca tatttcgcaa tttgttcttt
    13381 gctacctccg aaatagaaag tgttatgccc taaaccttgg tgaacaccga gtagggtagc
    13441 taaggaaata tcaaatcgcg cgagtaggta agacatgaaa aactgaaata gttgactagg
    13501 cattttggcg tttggacgat ccttgtaaag taatggattg ttaaaataat ttaattctcc
    13561 cagatcttta aaatagtcct cgggtacagt agcgtctatc caatgttgat taatattttc
    13621 acggtactta ctttctagca atgaatctac ttgttgtaaa aattcgactt caccgtctgt
    13681 taaaccttta gcaatactaa gtacatcttc aggaaataat gtttttaaga ccgtttcttt
    13741 ttcaaatgtc atataaattc ctcctaaaaa taatatgaat actaatgtga aatgcattta
    13801 attcaaaaac aacacgcttt atttgtaaac gcttacacta aatgtcaaaa atttttatca
    13861 cctttaaagt gtttgtgaga ctttgtcatt catcatttgt cgaatcgcaa gtttatctgg
    13921 tttctgcgta ctgtttaacg gcatatgtgt cactggtaca tacattcttg ggactttata
    13981 acctgctaaa cgacttcgca tatgttgatc taaaatttca gcgtaatgag gttcatcttc
    14041 gcgaagtata atggctgcag caattgattc accatatttt ggatgatcat agccaacgac
    14101 cacacaccgg tctactagtg gatgctcagc taaagcattt tcgacttcgg atggtaagac
    14161 attttcgcca ccagttatga ttaattcttt tttgcgttca ataataaata tatcgccatc
    14221 gtcgtccatc ttcgctaagt caccagttaa taaataacga ccatgaaatg ctttggcagt
    14281 ctctgctggt ttattccaat atcctggcgt gacattttta gccttaattg caagttcgcc
    14341 aatctcacca gtaggtactt cctcaccgtt atcatcaagg atacgtgcat caacgaacat
    14401 gactgcttta ccaatactca ttggcttacg ttttgaattt tccggtgtat taacaagtac
    14461 aagaggtgct tcagttaaac catagccgtt aataatgttt atgccatatt gtttaaaagc
    14521 tgcttggata cttggtaatg gttgtgaacc accttggatg atataatcca tagctttaaa
    14581 attttcagga ttaaaattac tagcacgtag cgtactataa tacattgtcg gaatcatgat
    14641 aataaatgta gggtgatatt gtgcaatcat gtcattcaat tcttcgccgt taaagtaacg
    14701 ttgaagaata agtgtgccac ctgacatcaa tactggtaat acagtatcgt taaaccctaa
    14761 aacatggaac attggtgttg atacaatcgt aatatagttt gaattgaact tatacgtcag
    14821 ctctaggttt gcaccgttat gaacaaatga ttcatatgag aacatcacac ctttaggtga
    14881 tccggttgta ccacttgtat aaattaatgc tgcaagatct tgtggttcga caggtgttgc
    14941 ttgaaaaggt tggtgataat ctggatttac gatttcatca tattgcgcta catcaatatc
    15001 catatgcaat aagttttggt caatatcggt gagtgaactt aaatgttttt cagcatagaa
    15061 gagcagtttt aattgtgcat cttccacaat ggctgcaatt tcttttgggt taagccgcca
    15121 attcaatggt aaaaaaaccg cacctgtttt aaaacaagca aacaataaat ctaatattgc
    15181 aatatcattt ggcgcaaaaa taccgataac atcgcctttt ttaacacctt gagatgttaa
    15241 ataatgtgcc atattatcag cgcgtgcatt gagttgttgg tatgtccaag atgtttgttt
    15301 tgcgtgatca ataacggcag gcttgtcatc atcgaagtct gaacgcgttt ttatccaatc
    15361 gaaattcatt agtatacccc ctttagcttc actttcatac tttatgaatt gattgtttaa
    15421 gttgtcccca tttttctttg taaatgctgg tatcaattaa ttttaaatga tcagcaataa
    15481 ttggtttaaa agccatttga ttcaaaatat ctttatgcaa atcaagacct ggtgcaattt
    15541 caattagttt caagccttga ttggtgagtt cgaatactgc acgctcagta acaaaataga
    15601 cttcttgctc gagtgattgt gaatattgtg cattaaagtc gatatggctc acatctgata
    15661 caaatttctg gttttgtcct tcagtttcaa tgtttaatcg ttgattatgg catgagacat
    15721 gactgccagc tacaaaagta cctgaaaaga taattttatt tacagattgc gtaatgtcta
    15781 taaagccacc acatccattt agtcggtcat tgaagtaaga cacgttgaca ttgccgtatt
    15841 gatcaacctc agcaaagcta agataggcaa ctgatacacc attgttataa ataaaatccc
    15901 atgctcgatc atgaggcatg cgcacatctg cattgtaatt cataccaaaa tgttcacgac
    15961 tcccaacgaa tccaccgaaa atgccaacat ctaaaatcgg ttgcacatca tgttcaacac
    16021 attcttcgtg caataaatta gagagttcat tattgatgcc ataaccgatg ctaattgtat
    16081 cgccataagt taaaaactga gcagcacgtc ggagaatcaa tttgcgacta ttaaaaggta
    16141 atgcgggttc aggtattcca tcaattcgtt cttctccaga caaggctggt aaataatgac
    16201 tctgaattac ttggcggtga ttcttttcat cttctgtgac gtatacataa tcgacaagat
    16261 ttcctgggat aacaacttca ttcggtttta gttgatatcc gtcaactaaa gctttaactt
    16321 gtacaataac tttcccatga ttggctttcg cgtttaatgc gacatgataa cactcgctca
    16381 agtacgcttc ttgagttaaa taaatgttac cttgttgatc tgcgtatgtt cctctcagta
    16441 gtgccacatc aacgctaggg aatgtgtaat gtaagtatgt ttcatcgttg atggttacta
    16501 atgaaactaa atcatcagtt gttcgtgtat ttactttacc gccaccgtat ctaggatcaa
    16561 cagctgtgtt taatccgatt ttagtaataa ctccaggtaa taattgatta ctctgacgat
    16621 aatgagttgc aatgatacct tgtggtaaaa aataagcttc aatgtcatta tttttcattg
    16681 cttgtgccgt tttggaagaa gccgttaaaa tgctcataat gacacgttta atcatgcgac
    16741 gttctataaa atcatctaaa tccggtgcgg cacctaaact atggatatca ttcgctaata
    16801 taaacgttaa atctttgggc gtatgatatg tgtcatgttg cgctaacaca gcacgtagaa
    16861 cttcggcggg taagttggct acagctaatg ctggtaaacc aatcacatca ccatctttaa
    16921 tgatatgttg taagtcgtgc catgtgattt gtttcaagca agtcacctcc atcacatttg
    16981 ataaaatata gcgtttttac actttgtgta aacccttaca agaaatataa cataacgacg
    17041 tttaaaatca attagaaata tctttttatt ctgataatag acacagtata gacacatttt
    17101 gatggttgat aacaattgta atatcaaggg tttgtaatga attgaatatc attaaaatac
    17161 ttatataaaa atattgctcg gaatataaaa agttaaatag gttttgattt ttaaatatga
    17221 aatacaaagc gcccaatcga acaaagtatt tatattaaaa tatggaaaat ccatcaatat
    17281 taaattaaaa tagttttatt atgaaaagtg aaagtaggta agtctatgga aggtcttaat
    17341 catcgaagaa atacagaaaa agaagagaca acacaaacgc aatcagttgc acctaataca
    17401 ggtgaagagg ggatgtcatc agcaagtaca caatcaacta agacgtccga catacataat
    17461 gaatctatca ataaacaaat ggaagccaaa gcgcatgaaa cagcgcaaaa tgcagattta
    17521 aaaaccgaag caagaagttt atttgataat gcaaccaaat caatcggtag actagccggc
    17581 aatgatgaaa gcttaaatct taatttaaaa gatatgtttt ctgaagtatt taagccgcat
    17641 actaaaaacg aagcagatga aatatttata gcgggtactg ctaaaactac gccagcaatt
    17701 tgtgacatat cagaagaatg ggggaagcca tggctctttt ctcgagtatt catcgctttc
    17761 acagtaacat ttattggatt atgggtcatg gcagcgattt ttaataacac taacgcgatt
    17821 ccgggtctca tttttatagg ggctttaaca gtaccattat cgggtttgtt cttcttttat
    17881 gaatcaaatg cgtttaaaaa tattagcatt tttgaagtta ttatcatgtt ctttattggc
    17941 ggcgtatttt cattactaag tacgatgtta ttatatagat ttgtcgtttt tagtgatcaa
    18001 ttcgaaaggt ttggttcttt aacatttttc gatgcatttt tagtaggatt agttgaagaa
    18061 actggaaaag cactcattat tgtttatttc gtcaataaat tgaaaacaaa taagattttg
    18121 aatggattat taatcggtgc tgctattggt gcagggttcg cagtttttga atcagcaggt
    18181 tatattttga atttcgcttt aggggaaaat gtcccattat tagatattgt cttcacacgt
    18241 gcgtggactg cgattggtgg tcatttagtt tggtcagcga ttgttggtgc tgcaatagtt
    18301 attgcgaaag aacagcatgg ctttgaattc aaagatattt ttgataaacg ctttttaata
    18361 ttctttttat cagccgttgg tttacatggc atttgggata catctttaac tgtacttggc
    18421 agtgatacgt taaaaatatt tattttaatc gttattgtgt ggatacttgt attcatttta
    18481 atgggggcag gtttaaaaca agtgaattta ctgcagaaag aatttaaaga acaacagaaa
    18541 aaagtagacg aataataatt aaagcttatg ttgctcatat gtttgtgaca taagctattt
    18601 ttataatttg tctttaaaag agtggaatag gaatactttt cggagttaaa aaagtgtttc
    18661 acgttaaaca aatagtgaca attagattta tataaaatga acatgattca ctgaaagtat
    18721 gtaataatca ttttattgaa attcatcaaa cagaaattaa tacaatcata taagcaaatt
    18781 aaaccacgcc ataatcatat tggatgactt cggcgtggtt tttatagttg aagcagggct
    18841 gagacataaa tcaatgtccc acactccctt atcgttcaat cgttgttcga taatcgatta
    18901 aatagatacc ttcaggtgtt actttataat ttttaacctt agcgttagca gcgactattt
    18961 gatcgttgta agcaatataa ctgtttggta catctcgact tgataattta ataatatcat
    19021 tagaaatatt gtgacgttcc ttaacatcta cagtatgatt caattgatta attaaatcat
    19081 cgacgttgct attattgtag tctcctttat taatagcacc atctttttta tatgcttgat
    19141 taaagaaata acctgtatca ccgcgaggga ttgtcccaaa actatacatc gttgcatccc
    19201 atgcagaacg gtcttttaag taaccttcta tgtcatcaac acttttaatg tcgatttcaa
    19261 tatttgcttt tttagcatct gattgtaata cttgcgcaat tttcgatagc tctggacgac
    19321 cgtcatacgt aattaactta atttttaaag ggtgttcttt tgtataacca tctttagcta
    19381 ataacgtttt tgcttgttcg atattttgtt tggttaactt aggttcttta atatatggaa
    19441 ttttatcatt aaatggactc gttgcaggtt tcgcataacc ttgataaata tgatctgcaa
    19501 taccttgtct atcaatgata tgatctaatg cttcacgaac ggatttagtc atttttttat
    19561 tagtatgatt atacataagt aaagaagttc taaatccaga ttcttttgac acttttaaat
    19621 tttgattatt ttctatgtct tgaactttat taactgggac atcagttatt aaatcatctt
    19681 tttgagattc taaatttctg acgcgattat tgccgtcttc ttggtacgtc acagtaatat
    19741 gatcaagttt gggtttacct tgccaatagt ccttaaaatt cgacaatgat atttttcgag
    19801 attgcttata atcttttatt tggtaagggc ctgtaccaac aggagtttga ttaacatctg
    19861 atttagcatc tgtatcataa attgccataa aaggattagc taattcagat acaagttcag
    19921 ggtaagcgga gttggtttta attgtcagtt tttgaccttt agcggtaatt gatgatattg
    19981 gtaatgaata tttgaccaag tcgctttttt tcatgctatt ttcaaggcta gatttcactt
    20041 tttctgcagt caatttttga ccgttttgaa atttaatatt atcttttaat tctatatcta
    20101 acgttgtatc atttggttga tgatacgatt tcactaatgc tttttctatt tttccttgat
    20161 catttgtttt aaataatgat tctgcagcac caatcttaac tggtacatct gtttcataag
    20221 gtgcaataga ctttgttttt aacggtaacg aaatatttaa gtctttgcca gatgaatgca
    20281 ttgagccaca tcctgataac actaatactg ctgaaaatat agttgctagt cttttaaact
    20341 tcatttcatt aacactctct ttctaattac
//
LOCUS       DAMZKC010000034        20367 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.034, whole genome shotgun sequence.
ACCESSION   DAMZKC010000034 DAMZKC010000000
VERSION     DAMZKC010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 20367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20367
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.034"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            250..741
                     /gene="lspA"
                     /locus_tag="POB55_000918"
     CDS             250..741
                     /gene="lspA"
                     /locus_tag="POB55_000918"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDI6499892.1"
                     /translation="MHKKYFIGTSILIAVFVVIFDQVTKYIIATTMKIGDSFEVIPHF
                     LNITSHRNNGAAWGILSGKMTFFFIITIIILIALVYFFIKDAQYNLFMQVAISLLFAG
                     ALGNFIDRILTGEVVDFIDTNIFGYDFPIFNIADSSLTIGVILIIIALLKDTSNKKEK
                     EVK"
     gene            741..1658
                     /locus_tag="POB55_000919"
     CDS             741..1658
                     /locus_tag="POB55_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDI6499893.1"
                     /translation="METYEFNITDKEQTGMRVDKLLPELNSDWSRNQIQDWIKAGLVV
                     ANDKVVKSNYKVKLNDHIVVTEKEVVEADILPENLNLDIYYEDDDVAVVYKTKGMVVH
                     PSPGHYTNTLVNGLMYQIKNLSGINGEIRPGIVHRIDMDTSGLLMVAKNDIAHRGLVE
                     QLMDKSVKRKYIALVHGNIPHDYGTIDAPIGRNKNDRQSMAVVDDGKEAVTHFNVLEH
                     FKDYTLVECQLETGRTHQIRVHMKYIGFPLVGDPKYGPKKTLDIGGQALHAGLIGFEH
                     PVTGEYIERHAELPQDFEDLLDTIRKRDA"
     gene            2058..2585
                     /gene="pyrR"
                     /locus_tag="POB55_000920"
     CDS             2058..2585
                     /gene="pyrR"
                     /locus_tag="POB55_000920"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HDI6499894.1"
                     /translation="MSERIIMDDAAIQRTVTRIAHEILEYNKGTDNLILLGIKTRGEY
                     LANRIQDKIHQIEQQRIPTGTIDITYFRDDIEHMSSLTTKDAIDIDTDITDKVVIIID
                     DVLYTGRTVRASLDAILLNARPIKIGLAALVDRGHRELPIRADFVGKNIPTSKEETVS
                     VYLEEMDQRNAVIIK"
     gene            2803..4110
                     /locus_tag="POB55_000921"
     CDS             2803..4110
                     /locus_tag="POB55_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HDI6499895.1"
                     /translation="MQNDEMFERTVKPVLDVNEKPQPAQWAFLSLQHLFAMFGATVLV
                     PFLTGLPISAALLASGIGTLLYILITKAQIPAYLGSSFAFITPIITGLSTHSLGDMLV
                     ALFMSGVMYVIIGILIKLSGTAWLMKLLPPVVVGPVIMVIGLSLAPTAVNMAMYENPG
                     DMKGYNISFLIVAMITLLVTIVVQGFFKGFLSLIPVLVGIIVGYVVAIFMGIVKFDAI
                     MSAKWIDFPHIYLPFKDYVPSFHLGLVLVMIPIVFVTVSEHIGHQMVLNKIVGRNFFE
                     KPGLDKSIIGDGVSTMFASIIGGPPSTTYGENIGVLAITRIYSIYVIGGAAVIAIVLA
                     FIGKFTALISSIPTPVMGGVSILLFGIIAASGLRMLVESKVDFANNRNLVIASVILVV
                     GIGNLVFNLKEIGINLQIEGMALAALSGIILNLILPKEKKQNN"
     gene            4138..5019
                     /locus_tag="POB55_000922"
     CDS             4138..5019
                     /locus_tag="POB55_000922"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HDI6499896.1"
                     /translation="MNHLLSMEHLSTDQIYKLIQKASQFKSGERQLPNFEGKYVANLF
                     FENSTRTKCSFEMAELKLGLKTISFETSTSSVSKGESLYDTCKTLESIGCDLLVIRHP
                     FNNYYEKLANINIPIANAGDGSGQHPTQSLLDLMTIYEEYGYFEGLNVLICGDIKNSR
                     VARSNYHSLKALGANVMFNSPNAWIDDSLEAPYVNIDDVIETVDIVMLLRIQHERHGL
                     AEETRFAADDYHQKHGLNEVRYNKLQEHAIVMHPAPVNRGVEIQSDLVEASKSRIFKQ
                     MENGVYLRMAVIDELLK"
     gene            5037..6311
                     /locus_tag="POB55_000923"
     CDS             5037..6311
                     /locus_tag="POB55_000923"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HDI6499897.1"
                     /translation="MKLIKNGKVLQNGELQQADILIDGKVIKQIAPAIEPSNGVDIID
                     AKGHFVSPGFVDVHVHLREPGGEYKETIETGTKAAARGGFTTVCPMPNTRPVPDSVEH
                     FEALQKLIDDNAQVRVLPYASITTRQLGKELVDFPALVKEGAFAFTDDGVGVQTASMM
                     YEGMIEAAKVNKAIVAHCEDNSLIYGGAMHEGKRSKELGIPGIPNICESVQIARDVLL
                     AEAAGCHYHVCHVSTKESVRVIRDAKRAGIHVTAEVTPHHLLLTEDDIPGNNAIYKMN
                     PPLRSTEDREALLEGLLDGTIDCIATDHAPHARDEKAQPMEKAPFGIVGSETAFPLLY
                     THFVKNGDWTLQQLVDYLTIKPCETFNLEYGTLKENGYADLTIIDLDSEQEIKGEDFL
                     SKADNTPFIGYKVYGNPILTMVEGEVKFEGDK"
     gene            6313..7413
                     /gene="carA"
                     /locus_tag="POB55_000924"
     CDS             6313..7413
                     /gene="carA"
                     /locus_tag="POB55_000924"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDI6499898.1"
                     /translation="MQSKRYLVLEDGSFYEGYRLGSDNLTVGEIVFNTAMTGYQETIS
                     DPSYTGQIITFTYPLIGNYGINRDDFESLVPTLNGIVVKEASAHPSNFRQQKTLHDVL
                     ELHQIPGIAGVDTRSITRKIRQHGVLKAGFTDRKEDIDQLVKHLQQVELPKNEVEIVS
                     TKTPYVSTGKDLSVVLVDFGKKQNIVRELNVRGCNVTVVPYTTTAEEILAMAPDGVML
                     SNGPGNPEVVECAIPMIQGILGKIPFFGICLGHQLFALSQGASSFKMKFGHRGANHPV
                     KNLETGKVDITSQNHGYAIDIDSLKSTDLEVTHLALNDGTVEGLKHKTLPAFSVQYHP
                     EANPGPSDSNYLFDDFVAMMTNFKEKERHINA"
     gene            7406..10579
                     /gene="carB"
                     /locus_tag="POB55_000925"
     CDS             7406..10579
                     /gene="carB"
                     /locus_tag="POB55_000925"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HDI6499899.1"
                     /translation="MPKRNDIKTILVIGSGPIIIGQAAEFDYAGTQACLALKEEGYRV
                     ILVNSNPATIMTDKEIADKVYIEPLTHDFIARIIRKEQPDALLPTLGGQTGLNMAIQL
                     HESGVLQDNNVQLLGTELTSIQQAEDREMFRTLMNDLNVPVPESDIVNTVEQAFKFKE
                     QVGYPLIVRPAFTMGGTGGGICHNDEELHEIVSNGLHYSPATQCLLEKSIAGFKEIEY
                     EVMRDKNDNAIVVCNMENIDPVGIHTGDSIVVAPSQTLSDVEYQMLRDVSLKVIRALG
                     IEGGCNVQLALDPHSFDYYIIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEM
                     LNPITGTSYAAFEPTLDYVISKIPRFPFDKFEKGERELGTQMKATGEVMAIGRTYEES
                     LLKAIRSLEYGVHHLGLPNGESFDLDYIKERISHQDDERLFFIGEAIRRGTTLEEIHN
                     MTQIDYFFLHKFQNIIDIEHQLKEHQGDLEYLKYAKDYGFSDKTIAHRFNMTEEEVYQ
                     LRMENDIKPVYKMVDTCAAEFESSTPYYYGTYETENESIVTDKEKILVLGSGPIRIGQ
                     GVEFDYATVHAVWAIQKAGYEAIIVNNNPETVSTDFSISDKLYFEPLTEEDVMNIINL
                     EKPKGVVVQFGGQTAINLADKLAKHGVKILGTSLENLNRAEDRKEFEALLRKINVPQP
                     QGKTATSPEEALANAAEIGYPVVVRPSYVLGGRAMEIVDNDKELENYMTQAVKASPEH
                     PVLVDRYLTGKEIEVDAICDGETVIIPGIMEHIERAGVHSGDSIAVYPPQTLTEDELA
                     TLEDYTIKLAKGLNIIGLINIQFVIAHDGVYVLEVNPRSSRTVPFLSKITDIPMAQLA
                     MRAIIGEKLTDMGYQEGVQPYAEGVFVKAPVFSFNKLKNVDITLGPEMKSTGEVMGKD
                     TTLEKALFKGLTGSGVEVKDHGTVLMTVSDKDKEEVVKLAQRLNEVGYKILATSGTAN
                     KLAEYDIPAEVVGKIGGENDLLTRIQNGDVQIVINTMTKGKEVERDGFQIRRTTVENG
                     IPCLTSLDTANALTNVIESMTFTMRQM"
     gene            10689..11381
                     /gene="pyrF"
                     /locus_tag="POB55_000926"
     CDS             10689..11381
                     /gene="pyrF"
                     /locus_tag="POB55_000926"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HDI6499900.1"
                     /translation="MKDLPIIALDFESKEKVNQFLDLFDESLFVKVGMELFYQEGPQL
                     INEIKERGHDVFLDLKLHDIPNTVGKAMEGLAKLNVDLVNVHAAGGVKMMSEAIKGLR
                     KHNQHTKIIAVTQLTSTTEDMLRHEQNIQTSIEEAVLNYAKLANAAGLDGVVCSPLES
                     RMLTEKLGTSFLKVTPGIRPKGASQDDQHRITTPEEARQLGSTHIVVGRPITQSDNPV
                     ESYHKIKESWLV"
     gene            11381..11992
                     /locus_tag="POB55_000927"
     CDS             11381..11992
                     /locus_tag="POB55_000927"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HDI6499901.1"
                     /translation="MAKEIAKSLLDIEAVTLSPNDLYTWSSGIKSPIYCDNRVTLGYP
                     LVRGAIRDGLINLIKEHFPEVEVISGTATAGIPHAAFIAEKLKLPMNYVRSSNKSHGK
                     QNQIEGAKSEGKKVVVIEDLISTGGSSVTAVEALKLAGAEVLGVVAIFTYGLKKADDT
                     FSNIQLPFYTLSDYSELIEVAENEGKISSEDIQTLVEWRDNLA"
     gene            12022..12234
                     /locus_tag="POB55_000928"
     CDS             12022..12234
                     /locus_tag="POB55_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499902.1"
                     /translation="MNDKTSNDLYGKIKHCNEFINHSNDSNLSSSHDVDESSTKQKHI
                     KNKTTIDHNDDLFKHVKDILRKQGQI"
     gene            12671..13072
                     /locus_tag="POB55_000929"
     CDS             12671..13072
                     /locus_tag="POB55_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDI6499903.1"
                     /translation="MDIPKITTFLMFNNQAEEAVKLYTSLFEDSEIITMAKYGENGPG
                     DPGTVQHSIFTLNGQVFMAIDANSGTELPMNPAISLFVTVKDTIEMERLFNGLKDEGA
                     ILMPKTNMPPYREFAWVQDKFGVSFQLALPE"
     gene            complement(13335..15032)
                     /locus_tag="POB55_000930"
     CDS             complement(13335..15032)
                     /locus_tag="POB55_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HDI6499904.1"
                     /translation="MAYDGLFTKKMVESLQFLTTGRVHKINQPDNDTILMVVRQNRQN
                     HQLLLSIHPNFSRLQLTTKKYDNPFNPPMFARVFRKHLEGGIIKSIKQIGNDRRIEIE
                     IKSKDEIGDTIYRTVILEIMGKHSNLILVDENRKIIEGFKHLTPNTNHYRTVMPGFNY
                     EAPPTQHKINPYDITGAEVLKYIDFNAGNIAKQLLNQFEGFSPLITNEIVSRRQFMTS
                     STLPEAFDEVMAETKLPPTPIFHKNHETGKEDFYFIKLNQFNDDTVTYDSLNDLLDRF
                     YDARGERERVKQRANDLVRFVQQQLHKYQNKLAKLIEEYEQSKNKDTEQLYGELITAN
                     IYRIKQGDKEVTALNYYTNEEVVIPLNPTKSPSANAQYYYKQYNRMKTRERELQHQIQ
                     LTKDNIDYFSTIEQQLHHISVHDIDEIRDELAEQGFMKQRKNQTKKKKAQIQLQHYVS
                     TDGDDIYVGKNNKQNDYLTNKKAKKTHTWLHTKDIPGSHVVIFNDAPSDTTIKEAAML
                     AGYFSKAGNSGQIPVDYTLIKNVHKPSGAKPGFVTYDNQKTLYATPDYELIQKMKQS"
     gene            15307..15930
                     /gene="gmk"
                     /locus_tag="POB55_000931"
     CDS             15307..15930
                     /gene="gmk"
                     /locus_tag="POB55_000931"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDI6499905.1"
                     /translation="MDNEKGLLIVLSGPSGVGKGTVRKRIFEDPSTSYKYSISMTTRQ
                     MREGEVDGVDYFFKTRDAFEALIKDDQFIEYAEYVGNYYGTPVQYVKDTMDEGHDVFL
                     EIEVEGAKQVRKKFPDALFIFLAPPSLEHLRERLVGRGTESDEKIQSRINEARKEVEM
                     MNLYDYVVVNDEVELAKNRIQCIVEAEHLKRERVEAKYRKMILEAKK"
     gene            15930..16148
                     /locus_tag="POB55_000932"
     CDS             15930..16148
                     /locus_tag="POB55_000932"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDI6499906.1"
                     /translation="MLNPPLNQLTSQIKSKYLIATTAAKRAREIDEQPETELLSEYHS
                     FKPVGRALEEIADGKIRPVISSDYYGKE"
     gene            16364..17563
                     /gene="coaBC"
                     /locus_tag="POB55_000933"
     CDS             16364..17563
                     /gene="coaBC"
                     /locus_tag="POB55_000933"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HDI6499907.1"
                     /translation="MKKILLAVTGGIAAYKAIDLTSKLTQSGYEVRVMLTNHAQKFVT
                     PLAFQAISRNAVYTDTFIEENPSEIQHIALGDWADAIIVAPATANTIAKLSVGIADDL
                     VTSTLLATETPKFIAPAMNVHMYENKRTQQNINILKEDGYHFIEPGSGFLACGYVAKG
                     RMEEPLQIVSVIDAHFQNSNRLANSSFQDKRALVTAGPTIEVIDPVRFVSNRSSGKMG
                     YAIAEALRNRGAIVTLVAGPTTLEDPKDIEVIHVQSAEEMFEQVTSRFDEQDIVVKAA
                     AVSDYTPVDVLEHKMKKQDGDLSVSFKRTKDILKYLGEHKTSQYLIGFAAETEDIENY
                     AQQKLRKKNADVIISNNVGDMSIGFSSDDNELTMHFKNNEKVNIKKGKKVVLAAQILD
                     ELETRWQ"
     gene            17563..19971
                     /gene="priA"
                     /locus_tag="POB55_000934"
     CDS             17563..19971
                     /gene="priA"
                     /locus_tag="POB55_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDI6499908.1"
                     /translation="MIAKVIVDVASKSVDYKFDYIIPEQLESVIQPGVRVIVPFGPRT
                     IQGYVMEVTAEPDAQLDVSKLKKIIEVKDIQPELTSELIALSEWMGSTHVIKRISMLE
                     VMLPSAIKAKYKKAFKMKDDIELPSALLQKFDKHGYYYYKDAQKNNDIQLLMKLLKDD
                     IVEEKTILTQNITKKTKRAVRVIEGYHPDEVLAKLEKVIKQYDLYAYLSEEQHKTIFL
                     TDIEDMGFSKSSLDGLIKKGYVEKYDAVVERDPFKDRVFEQESKQQLTEDQYKAYEAI
                     KAKIVSQEQETFLLHGVTGSGKTEVYLQTIEDVLSQGKQAMMLVPEIALTPQMVLRFK
                     RRFGDDVAVLHSGLSNGERYDEWQKIRDGRARVSVGARSSVFAPFKNLGLIIIDEEHE
                     STYKQEDYPRYHAREIAQWRSEYHHCPVILGSATPCLESYARAEKGVYHLLSLPNRVN
                     QQALPEIDIVDMREELSEGNRSMFSKDLREAIQLRLDRQEQVVLFLNRRGYASFMLCR
                     DCGYVPQCPNCDISLTYHKTTDLLKCHYCGYQETPPNQCPNCESEHIRQVGTGTQKVE
                     ELLQQEFEDARIIRMDVDTTSKKGAHEKLLTEFEKGNGDILLGTQMIAKGLDYPNITL
                     VGVLNADTMLNLPDFRASERTYQLLTQVAGRAGRHEKAGQVIIQTYNPDHYSILDVQK
                     NDYLTFYRQEMEYRKLGKYPPYYYLINFTISHKEMKKVMEASQHVHKILLQHLTEKAL
                     VLGPSPAALARINNEFRFQILVKYKSEPGLLQAIQFLDDYYHEKFIKEKLALKIDIDP
                     QMMM"
BASE COUNT         7205 a         2947 c         3829 g         6386 t
ORIGIN      
        1 gtacattata aaatacatat caaaaaagct gatttctatc aaataattaa tagaaaactg
       61 cttttttaaa ttgcctaaga acttaatatc ccagcctgct ttcctaattt ttaatgtcaa
      121 aacatagtag tttatcaagt attgagtgag taacattaga tttaatgtaa tatgcttact
      181 ttttttatta gcaggtgtaa gctataatat aaagagttgt ctcatggacg attgattgga
      241 ggaacgaaaa tgcacaaaaa atattttatt ggcacttcca ttttaatagc agtatttgtc
      301 gttatatttg accaagttac taaatatatt atagctacta caatgaaaat tggagattca
      361 tttgaagtga taccgcactt tttaaacata acatcacatc gaaataatgg tgctgcatgg
      421 ggaatattga gtggaaaaat gacatttttc tttattatta ccattattat attaatagcc
      481 ttagtatatt tctttattaa agatgctcaa tataatttgt ttatgcaagt tgctattagt
      541 ttactttttg caggtgcact tggaaacttt attgatagaa ttttaacagg agaagttgtt
      601 gactttattg atacaaatat ttttggttat gattttccaa tatttaatat cgcagattca
      661 agtttaacaa ttggtgtaat attaattatt attgccttat taaaggatac ttccaataaa
      721 aaggagaagg aggttaagta atggagactt atgaatttaa cattacagat aaagaacaaa
      781 caggtatgcg tgtagataag ttgctgcctg aattaaatag tgattggtct cgtaaccaga
      841 tacaagattg gattaaagca ggtttagtcg ttgcaaacga taaagttgtt aaatctaatt
      901 ataaagtgaa acttaatgat catatagttg tcactgaaaa agaagtggtt gaagctgata
      961 ttctacctga aaatttaaat ttagatattt attatgaaga tgacgatgtt gcagttgtat
     1021 ataaaacgaa aggcatggta gttcatccat caccagggca ttataccaat acattagtta
     1081 atggtttaat gtatcaaatt aaaaatttgt caggtattaa tggagaaatt cgtccaggta
     1141 ttgttcaccg tatagatatg gatacttctg gtttattaat ggttgctaaa aatgatattg
     1201 ctcatcgtgg gcttgtagaa caattaatgg ataaatctgt taaaagaaaa tatatcgctt
     1261 tagttcacgg gaatattcct catgattacg gtacaatcga tgcgccaatt ggtagaaaca
     1321 aaaatgatcg tcaatctatg gctgttgttg atgatggtaa ggaagcagtg acacatttta
     1381 acgtactaga acattttaaa gattatacgc ttgttgaatg tcaacttgaa acaggacgta
     1441 cgcatcaaat ccgtgtgcac atgaaatata ttggcttccc attagttggt gatccaaagt
     1501 atggaccgaa aaagacattg gatattggtg gtcaagctct acatgctgga cttattggat
     1561 tcgaacatcc agtaacaggt gaatatattg aaagacatgc tgaattacca caagactttg
     1621 aagatttatt agatacaatt cgaaaaagag atgcataatt gtgttgtgct ataattacga
     1681 taacattatt atgtaaacta taagtattta tcgttttcgt ttttttaaaa tgaaaacaca
     1741 ttataaaata gattaaggtt aacattttta ctgagatcgg ttaatttttc aatatagatt
     1801 gacaaagcgc ctgattagta ttatccttat aacagtttaa gtacaacata aagagtcttt
     1861 aattgagcgt ccagagagac gtcaaagaca tgaaaatata gatatatact aaaatgatca
     1921 taacactttc acatatgcaa gaataagtag taatactacg attcttctat gtaaataggc
     1981 tgattgtata tactaaacct catgtcgata ttaaagacat gaggtttttt gtttctaaat
     2041 tttgaaaggg tgagaatatg tctgaacgta tcataatgga tgatgccgca atacaacgta
     2101 cagtgacgag aatcgctcac gaaattttgg agtataacaa aggtactgat aatttaattc
     2161 ttttaggtat caaaacaaga ggtgaatatt tagcgaatcg tatacaagat aaaattcatc
     2221 aaattgagca acaacgtata cctactggaa caattgatat tacatacttt agagatgata
     2281 tagagcacat gtcatcactt acgacaaaag acgcaataga catcgacaca gatattacag
     2341 ataaagtagt catcattatt gatgatgtgc tgtatactgg tcgaacggtt cgtgcttcac
     2401 ttgatgctat tttgctaaat gctagaccta ttaaaattgg tttagctgct ttggttgatc
     2461 gaggacatcg tgagttacca attcgagcag attttgttgg taaaaatata cctacttcta
     2521 aagaggaaac ggtaagtgtc tatttagaag aaatggatca aagaaatgca gttataatta
     2581 aataataccc cttttaattc agtacgagag actgaaaaag gtgggggagt tgaatgataa
     2641 ataagttttc atatgcttgt ttatctaata ataccctcat gttcgacaac tataagtcaa
     2701 tttctatgga cgaaaatgac ttgtagcaac ttgtcacact ttttgtctct ttacatatac
     2761 tgatgtaaag agattttttt attatcggag gaaatgaata ttatgcaaaa tgatgaaatg
     2821 tttgaacgaa cagtaaaacc tgtactagat gtaaatgaaa aaccacaacc agcgcaatgg
     2881 gcatttttaa gcttacaaca tttatttgcg atgtttggcg caacagtact agtaccattc
     2941 ttaacaggat taccaatatc cgcagcgtta ctagcttcgg gaatcggtac attactttat
     3001 atcttaataa cgaaggcgca aataccagca tacttgggat ctagctttgc atttatcacg
     3061 ccaattatca cgggattaag tacgcatagc ttaggagaca tgcttgtagc attattcatg
     3121 agtggtgtta tgtacgtcat catcgggatt ctaatcaaat taagtgggac agcatggtta
     3181 atgaaattat taccaccagt tgttgttgga ccagtcatca tggtaattgg tttaagctta
     3241 gcgcctactg cagtcaatat ggcaatgtat gaaaatcctg gagatatgaa aggttacaat
     3301 atcagtttct taattgttgc aatgataacc ttgcttgtaa caatagtcgt tcaaggattt
     3361 tttaaaggat tcctatcttt aattccagta cttgtaggta ttatcgtagg ttatgtggta
     3421 gcgattttta tggggattgt gaaatttgat gcaattatgt cagcaaaatg gatagatttc
     3481 cctcatattt atctgccatt taaagattat gtaccttcat ttcacttagg acttgtactg
     3541 gtaatgattc cgattgtgtt tgtaacagta agtgaacata ttgggcacca aatggtattg
     3601 aataaaatcg taggtagaaa cttctttgaa aagccaggac ttgataaatc aatcattggt
     3661 gatggtgttt ctacaatgtt tgccagtatt attggtggac caccaagtac aacatacggt
     3721 gaaaatatcg gtgtattagc gattaccaga atatacagta tttacgtcat tggtggtgca
     3781 gcagttatag caattgtttt agcattcatt ggtaagttca ctgcattaat ttcttctata
     3841 cctacaccag ttatgggagg agtatctata ttacttttcg gtattattgc agcaagtggc
     3901 ttaagaatgc tagttgaaag caaagtagat tttgcgaaca atcgaaattt agttatagct
     3961 tcagtaattt tagttgtagg tatcggtaat ttagtattta acttaaaaga aattggtatc
     4021 aaccttcaaa ttgaggggat ggcattagct gcactttcag gaattatttt gaacttaatc
     4081 ttacctaaag agaaaaaaca aaacaattaa gatttacaaa ttaaggaggg cgcttttatg
     4141 aatcatttat tatcaatgga acatttatct acagatcaaa tatacaaact tatccaaaag
     4201 gcaagtcaat ttaaatctgg tgaacgtcaa ctaccaaact ttgaagggaa atatgtcgca
     4261 aatttattct ttgaaaattc tactcgaaca aaatgtagtt ttgaaatggc agaacttaag
     4321 ctagggttaa aaacgattag ctttgaaaca tcaacatcat ctgtttcaaa aggtgaatct
     4381 ttatatgaca catgtaaaac tttagaaagt attggctgtg atttattagt cattagacat
     4441 ccgtttaata actactatga aaaattagcg aatattaaca tcccaattgc gaatgctggt
     4501 gatggtagtg gacaacatcc aacacaaagt ttacttgatt taatgacgat atatgaagaa
     4561 tatggatatt ttgaaggctt gaatgtattg atttgtggag acattaaaaa ttcacgtgtc
     4621 gcacgtagta attaccatag tttaaaagca ttaggtgcaa acgtaatgtt taatagccca
     4681 aatgcttgga ttgatgattc tttagaagca ccttatgtaa atatagatga tgttatagaa
     4741 acagtagata tagttatgtt attaagaatt caacatgaaa gacatgggct tgcagaagaa
     4801 actagatttg cagcagatga ttatcatcaa aagcatggct taaatgaagt gcgctataac
     4861 aaattacaag aacatgctat tgttatgcat ccggcacctg tgaatagagg agtagaaata
     4921 caaagcgatt tagtagaagc ttcaaaatca agaattttta agcaaatgga aaatggcgtt
     4981 tacttaagaa tggcagtcat tgatgaatta ttaaaatagg taaggggacg aaaatgatga
     5041 aattaattaa aaacggtaaa gtattacaaa atggcgaatt acaacaagca gatattttaa
     5101 ttgatggtaa ggtaattaaa caaattgcac ctgcaattga accaagcaat ggtgttgaca
     5161 tcatagatgc gaaaggtcac tttgtgtcac ctggatttgt cgatgttcat gttcatttac
     5221 gtgaacctgg tggtgaatat aaagagacaa ttgaaactgg tactaaagct gctgctagag
     5281 gcggatttac aactgtatgt ccaatgccta acacaagacc ggtaccagat tctgtagaac
     5341 attttgaagc tttacaaaaa ttaatcgatg acaatgctca agtacgtgta ttaccttatg
     5401 cttcaattac aacacgtcaa ttaggtaaag aattggttga tttcccagca ctagtaaaag
     5461 aaggtgcctt tgcgtttaca gatgacggtg taggagtaca aactgcaagc atgatgtatg
     5521 aaggtatgat tgaagctgca aaagtaaaca aagccatcgt agcacactgt gaagataatt
     5581 cattaatcta tggtggtgca atgcatgaag ggaaacgcag taaagagtta ggtataccag
     5641 gtattccaaa catttgtgaa tctgttcaaa tcgcaagaga tgtactatta gctgaagcag
     5701 caggttgtca ttatcatgta tgtcatgttt ctactaaaga aagtgttaga gtcattcgtg
     5761 atgctaaacg cgcaggcatt catgttacag ctgaagttac accacatcat ttattgttaa
     5821 cagaagatga tattcctggt aataatgcca tttataaaat gaatccacca ttgagaagta
     5881 ctgaagatag agaggctttg ttagaagggt tactagacgg tacaattgat tgtatcgcaa
     5941 cagaccatgc accacatgca cgtgatgaaa aagcgcaacc aatggaaaaa gcaccattcg
     6001 gaattgttgg tagtgaaaca gcattcccat tattatatac gcattttgta aaaaatggtg
     6061 attggacatt acaacaatta gtagattact taacaattaa accatgtgag acatttaatt
     6121 tagaatacgg cacattaaaa gaaaatggtt atgcagattt aacaatcatt gatttagata
     6181 gtgaacaaga aattaaagga gaagatttct tatcaaaagc agataataca ccatttatcg
     6241 gctataaagt ttatggaaat ccgatcttaa caatggttga aggcgaagtt aaatttgagg
     6301 gggataaata atatgcaaag caaacgttat ctagtgttag aagacggttc tttttacgaa
     6361 ggctaccgtt taggatctga taacttaact gtaggagaaa ttgtatttaa tacagcgatg
     6421 acaggttatc aagaaactat ttcagatcca tcatatacag gtcagatcat tacttttacg
     6481 tatccattaa tcggtaatta tggtatcaat agagacgatt ttgaatcatt agtacctaca
     6541 ttaaacggta ttgtagtgaa agaagcgagt gcgcatccaa gtaattttag acaacaaaag
     6601 acacttcatg acgttttaga attgcatcaa attccaggga ttgcaggtgt tgatacaaga
     6661 agtattacgc gtaaaattcg acaacacggt gtgttaaaag ctggttttac tgatcgaaaa
     6721 gaagatattg atcaacttgt caaacattta caacaagtag aattacctaa aaacgaagta
     6781 gaaatcgttt cgactaaaac accgtatgtt tcgacaggta aggatctaag tgtcgtactt
     6841 gtagactttg gtaagaagca aaatattgtt cgagaattaa acgtcagagg ttgtaacgtc
     6901 acagttgtac catatacaac tactgccgaa gaaattttag caatggctcc agatggcgtt
     6961 atgctatcaa acggaccagg taatcctgaa gttgtagaat gtgcgattcc aatgattcaa
     7021 ggaattttag ggaaaattcc gttctttggt atctgtctag gacatcaact ttttgcatta
     7081 tctcaaggag caagctcatt taaaatgaag tttggtcatc gtggtgcgaa ccatccagtt
     7141 aaaaatttag agactggaaa agttgatatt acgagtcaaa accatggata tgcaatagat
     7201 atagattcgt taaaaagtac tgatttagaa gttactcatc ttgcattaaa tgatggtact
     7261 gtagaaggtt taaaacataa aacattacca gcattttctg ttcaatacca tcctgaagca
     7321 aatccaggac cgtcagattc aaactatcta tttgatgatt ttgtagcaat gatgactaat
     7381 tttaaggaaa aggagcgtca tatcaatgcc taaacgtaat gatatcaaaa caattttagt
     7441 aatagggtct gggccaatta tcataggtca agcagctgaa tttgattatg ctggaacaca
     7501 agcatgtcta gctttaaaag aagagggata tcgagttatt cttgtaaatt caaatccagc
     7561 gacaatcatg actgataagg aaattgcgga taaagtatat atcgaaccgt taactcatga
     7621 ttttatagcg cgaattatac gtaaagagca acctgacgct ttacttccaa ctttaggtgg
     7681 tcaaacaggt ttaaacatgg cgattcaact acacgaaagt ggtgtgcttc aagataataa
     7741 cgtccaatta ttaggaactg agctaacatc aattcaacaa gcagaagacc gtgaaatgtt
     7801 tagaacatta atgaatgatt taaacgttcc tgtaccagag agtgacattg taaatacagt
     7861 agagcaagcc tttaaattca aagagcaagt gggatacccg ctaattgtta gaccggcatt
     7921 tacgatgggt ggtaccggag gcggtatttg tcataatgat gaagaattac atgaaatcgt
     7981 ctcaaatggt cttcattata gtccagcaac gcaatgttta ttagaaaaat ctatcgcagg
     8041 ttttaaagaa atcgaatacg aagtaatgcg tgataaaaac gataatgcca tcgttgtatg
     8101 taacatggaa aatattgatc cagttggtat tcatactggc gattcaattg ttgtggctcc
     8161 tagtcaaaca ttatcagatg ttgagtatca aatgttacgt gatgtttcat taaaagttat
     8221 tcgagcttta ggtatcgaag gtggttgtaa tgttcaatta gcattagacc cccattcatt
     8281 cgattattat attatagaag taaatccgcg tgtatcacgt tcatcagcgt tagcttcaaa
     8341 agcaacagga tatcctattg caaaattagc tgctaaaatc gcggttggtc taacattaga
     8401 tgaaatgtta aatccaatta caggaacatc ttatgcagcg tttgaaccaa ctttagacta
     8461 tgtgatttca aaaataccaa gatttccttt tgataaattt gaaaaaggag aacgagagct
     8521 tggcacacaa atgaaagcaa caggtgaagt tatggccatt ggtcgaactt acgaagaatc
     8581 attgttaaaa gcaattcgat cacttgagta tggtgtgcat cacttaggat taccaaatgg
     8641 tgaaagcttc gatcttgatt atattaaaga acgtatttca caccaagatg atgaacgatt
     8701 atttttcatc ggcgaagcaa ttagaagagg cacaacatta gaagaaattc ataatatgac
     8761 tcagattgat tacttcttct tacacaagtt ccaaaacatt attgatattg agcatcaact
     8821 aaaagagcat caaggtgatt tagaatatct taaatatgca aaagattatg gatttagtga
     8881 taaaacaata gcgcatcgct ttaatatgac ggaagaagaa gtatatcaat tgcgtatgga
     8941 aaatgatatt aaacctgttt acaagatggt tgatacttgc gcagctgaat ttgaatcttc
     9001 aacaccatat tattatggta catacgaaac tgaaaatgaa tccatagtta ctgacaaaga
     9061 aaaaatctta gtattaggct ctggaccaat tcgaatcggc caaggtgtag aatttgacta
     9121 tgcgacagtt cacgccgttt gggcaattca aaaagcaggg tacgaagcga taattgtgaa
     9181 taacaatcca gaaacagttt caacagactt ctcaatttct gacaaattat actttgaacc
     9241 tttaactgaa gaagatgtga tgaatatcat taatttagaa aaacctaaag gtgtcgttgt
     9301 acaatttgga ggacaaacag cgattaattt agcagacaaa ttggctaaac atggtgttaa
     9361 aatacttggt acttcactag aaaatctaaa tcgtgctgaa gatagaaaag aatttgaagc
     9421 actattaaga aaaattaacg tgccacagcc acaagggaaa acagctacat cacctgagga
     9481 agcattagcg aatgctgcag aaatcggata tccggttgta gtaagacctt cttatgtatt
     9541 aggtggtcgc gcaatggaaa ttgtagacaa tgacaaagag ttagaaaact atatgaccca
     9601 ggctgtaaaa gcgagtccgg aacatccggt actagtcgat agatatttaa ctggtaaaga
     9661 aattgaagtt gatgcgattt gtgatggaga aacggtcatt attccaggaa taatggaaca
     9721 tattgaacgt gctggtgtgc atagtggtga ctcaatcgct gtatatccac cacaaacttt
     9781 gacagaagac gagttagcaa cacttgagga ctatactata aaattagcta aaggtttaaa
     9841 catcattggc ttaatcaaca ttcaattcgt tatagctcac gatggtgtgt atgttttaga
     9901 agtaaatcca cgttctagta gaacggtacc attcttaagt aaaattactg atattccaat
     9961 ggcacaatta gctatgcgag caatcattgg ggaaaaacta acagatatgg gttatcaaga
    10021 aggggttcaa ccatatgctg agggtgtctt tgtgaaagca ccggtattta gtttcaataa
    10081 attgaaaaat gttgatatta ctttaggacc tgaaatgaag tcaacaggtg aagtgatggg
    10141 gaaagatact acattagaaa aggcgttatt caaagggtta acaggtagtg gtgttgaagt
    10201 taaagatcac ggtacagtat taatgaccgt cagtgacaaa gataaagagg aagttgttaa
    10261 attggcacaa cgcttaaatg aagttggcta taaaatttta gcaacgtctg gaacagctaa
    10321 taaattagct gagtatgaca tacctgcaga agtagtaggc aaaattggtg gcgaaaatga
    10381 tttattaaca cgtattcaaa atggtgatgt tcaaatcgtt ataaatacaa tgactaaagg
    10441 taaagaagta gaaagagatg gcttccaaat tagacgtact acagttgaaa atggtattcc
    10501 atgtttgaca tctttagata cagctaatgc cttaacgaat gtaattgaaa gtatgacatt
    10561 tacaatgcgt caaatgtaaa tcaatcaaac tgtatcggtg gggctgtaat taaccattta
    10621 cttaaagaag tttatattac agcctcatta ttttaatgaa tttcttaata taaagggaga
    10681 catatatgat gaaagattta ccaattattg cattagattt tgaatcaaaa gaaaaagtaa
    10741 atcaattttt agatttattt gatgaatcat tattcgtaaa agtaggtatg gaactttttt
    10801 atcaagaagg tcctcaatta attaatgaga taaaagaaag aggccatgat gtatttttag
    10861 atttaaaatt gcatgatatt cctaatacag taggtaaggc gatggaagga ctagctaaat
    10921 tgaatgttga tctggtaaat gttcatgctg ctggtggcgt aaaaatgatg tctgaggcca
    10981 ttaaaggatt aagaaaacat aatcaacata caaaaattat tgcagtaaca cagcttacgt
    11041 caacaacaga agacatgtta cgacacgaac aaaatataca aacatcgatt gaagaggccg
    11101 ttttaaatta tgccaagtta gcaaatgcag ctggtttaga tggcgttgtt tgttcacctc
    11161 ttgaaagtcg tatgttgact gaaaagttag gtacatcatt tttaaaagta acaccaggta
    11221 ttagacctaa aggtgcatct caagatgacc aacaccgtat tacgacaccg gaagaagcaa
    11281 gacagcttgg ttcgacgcat attgtagtcg gtagaccgat tacacaaagt gacaatccag
    11341 tcgaaagtta tcataaaatt aaagaaagtt ggttagtata atggctaaag aaattgcaaa
    11401 atcattatta gatattgaag ctgtaacatt atcaccaaat gatttatata catggagttc
    11461 aggtattaaa tcaccgattt actgtgataa ccgtgttacg ttaggttatc ctttagttcg
    11521 aggcgcaatc cgcgatggtt taattaactt aattaaagaa cactttcctg aagtagaagt
    11581 tatttctggt actgcaacag ctggtattcc acatgcagct tttattgctg aaaaattaaa
    11641 attaccaatg aattatgttc gttcatcaaa taagagtcat ggtaagcaaa atcaaatcga
    11701 aggtgctaaa agtgaaggta aaaaagtagt tgtgatagaa gatttaattt cgacaggggg
    11761 atcttcagtc acagcagttg aagccttaaa actagcaggt gcagaagtat taggtgttgt
    11821 agctatcttt acttacggtt tgaaaaaagc agatgataca tttagcaata ttcaactacc
    11881 tttttacact ttaagtgatt acagtgaatt aattgaagta gctgaaaatg aaggtaaaat
    11941 ttctagtgaa gatatccaaa cattagttga atggagagac aacttagcat aatatagaca
    12001 ctagaaggag gaattcaaca aatgaatgac aaaacatcta atgatttata tgggaagata
    12061 aaacattgta acgaatttat caatcattca aatgattcca atctatctag tagtcacgat
    12121 gtcgacgaaa gttcaacgaa gcaaaaacat ataaaaaata aaacaactat agaccataat
    12181 gatgatttat ttaaacatgt aaaggatata ttacgtaaac aaggacaaat ttaaaaacaa
    12241 aaaagctata gtgtttaata tcaggggctg cttaaccgca tccgaatatt acaaacacta
    12301 tagcttttat tatataaaaa atttattagc ggataattaa ctttattaaa atccaaccaa
    12361 ttaagaatac gagtaatagg actaatactg gaatcacata atgtaacata acgtccctcc
    12421 tttaacttaa ttttaattgt aatcaaattt gacaataagt caaaacatta atacctatga
    12481 taagtatcat ttattaacat atgtatcata tttttaatct tgcgtaattt ttatcgttaa
    12541 ctatgtgatt taatcaacag gccaataaga cgtttgtcat tcgtgcacat aacggtacga
    12601 cgcgcttttg tcattttaat tatgttaaga taatagtagt tatagaagtt caatctatag
    12661 gaggcatagc atggatattc caaaaatcac gacattttta atgtttaata accaagctga
    12721 agaagctgtt aaactataca caagcttatt tgaagatagt gagattataa caatggctaa
    12781 gtatggtgaa aatggacctg gtgatcccgg gactgtacaa cactcaatat ttacattaaa
    12841 tggacaagta ttcatggcga ttgatgctaa tagtggcaca gaattaccaa tgaatcctgc
    12901 gatttcatta tttgttacag taaaagatac tattgaaatg gaacgactat ttaatggatt
    12961 aaaagatgaa ggtgccattt taatgccaaa aacgaatatg ccaccataca gagagtttgc
    13021 ttgggttcaa gataagtttg gagtaagttt tcaattagca ttacctgagt aaaaggattc
    13081 gcacagcgtt gaatgataaa gaaacacttt ttcttatgca tgcgttttac ctatgtattt
    13141 ctattttaag tacacattag cattttagtt tcactcattt ttaaaatcac aaagataatt
    13201 tgtaacttaa atgatgatac gttatttaaa aacacgatac ttcgtttcaa tgaacgcatt
    13261 aaataataaa taaacacctc accataagaa aggactactt tcttttgtga ggtgtttatt
    13321 tgtcgacaac ttgattatga ttgtttcatt ttttgaataa gttcataatc aggtgtagca
    13381 tacaaagttt tttgattgtc atatgttaca aacccaggct ttgcacctga tggtttgtgc
    13441 acatttttaa ttaatgtgta atcaacaggt atttgtccag aattaccagc ttttgaaaag
    13501 tatcctgcta acatagccgc ttccttgatt gtcgtatcac ttggtgcatc attaaatata
    13561 acgacatgtg aaccaggaat atcttttgtg tgtaaccatg tgtgagtttt tttagctttt
    13621 ttatttgtta aataatcatt ttgcttgtta ttcttgccaa catatatatc gtcgccatca
    13681 gttgatacat aatgttgtaa ttgaatctgc gctttctttt tcttagtttg atttttacgc
    13741 tgtttcataa agccttgttc tgctaattca tctctaattt catcaatgtc atggacagaa
    13801 atatgatgta attgttgttc gattgttgaa aaataatcta tattgtcttt cgtcaattga
    13861 atttgatgtt gtaattcacg ttctctcgtt ttcatacgat tatattgttt ataataatat
    13921 tgagcatttg ctgatgggga ttttgtagga tttaaaggaa tgacaacttc ttcattcgta
    13981 taataattca atgccgtcac ttctttatcg ccttgcttaa ttcgatatat attagcagtg
    14041 atcaattcac catataactg ttcagtatct ttatttttag actgctcata ttcttcaatc
    14101 aacttcgcta atttattttg atatttgtgc aactgctgtt gaacaaatcg aactaaatca
    14161 ttcgcacgtt gtttaacgcg ttcacgttcg ccacgcgcat cataaaaacg atcaagtaaa
    14221 tcatttaatg aatcgtatgt aactgtatca tcattaaatt gatttaactt tataaaatag
    14281 aaatcctctt tacctgtttc atgattttta tgaaaaatag gagtaggtgg taacttggtt
    14341 tctgccatta cttcgtcaaa tgcttctggt aatgttgatg aagtcataaa ttgacgacga
    14401 ctaacaattt cattcgtaat taaagggcta aatccttcaa actgattcaa taattgttta
    14461 gcaatattac ctgcgttaaa atcgatatat ttcaacacct ctgcacctgt aatatcatac
    14521 ggatttattt tgtgctgagt aggtggtgct tcataattaa atcctggcat tactgtacga
    14581 tagtgattcg tatttggtgt taagtgttta aatccttcaa ttattttgcg attttcatct
    14641 actaaaatta agttactatg tttacccata atctcaagga tgacagtgcg gtaaatagta
    14701 tcgccaattt catctttact ctttatttcg atttcaatgc gacgatcatt accaatttgc
    14761 ttaatcgatt tgataatacc accttctaag tgttttctaa aaacacgcgc aaacatgggt
    14821 ggattaaatg gattatcata ttttttagta gttaattgta atcttgaaaa gtttggatgg
    14881 attgacaata acaattgatg gttttgtcta ttttgacgta caaccattag tatcgtgtca
    14941 ttatcaggtt gattgatttt gtgaacgcgt cctgttgtta aaaattgtag agactcaacc
    15001 attttctttg taaataagcc atcataagcc ataatttatc ttccaccttc ttcatatcat
    15061 tcgtttcatt ttaacatgat tgtaactttt cggtatagtt attgaaccat acattatgcc
    15121 ttacatttta cattatctaa aagtttgcaa ttcaatttat ttaattaaaa ttaatcgtgc
    15181 aatataacga tattgttaga cttaatagaa attatggcat gcaatttcaa atatgctata
    15241 caatataaag aacaatgtga tatcatattt aaataataga agattagctt agagaggtcg
    15301 taaggcatgg ataatgaaaa aggattgtta atcgttttat caggaccatc tggagtaggt
    15361 aaaggtactg ttagaaaacg aatatttgaa gatccaagta catcatataa gtattctatt
    15421 tcaatgacaa cacgtcaaat gcgtgaaggt gaagttgatg gcgtagatta cttttttaaa
    15481 actagggatg cgtttgaagc tttaatcaaa gatgaccaat ttatagaata tgctgaatat
    15541 gtaggcaact attatggtac accagttcaa tatgttaaag atacaatgga cgaaggtcat
    15601 gatgtatttt tagaaattga agtagaaggt gcaaagcaag ttagaaagaa atttccagat
    15661 gcgctattta ttttcttagc acctccaagt ttagaacact tgagagagcg attagtaggt
    15721 agaggaacag aatctgatga gaaaatacaa agtcgtatta acgaagcgcg taaagaagtt
    15781 gaaatgatga atttatacga ttacgttgta gttaatgatg aagtagaact tgcgaagaat
    15841 agaattcaat gtattgtaga agctgagcac ttaaaaagag agcgcgtaga agctaagtat
    15901 agaaaaatga ttttggaggc taaaaaataa tgttaaatcc acctttaaac caattaacgt
    15961 cacaaattaa atcaaagtat ttaattgcaa caactgcagc gaaaagagcg cgtgaaattg
    16021 atgaacaacc tgaaactgaa ttattaagtg aatatcattc atttaaacca gttggtagag
    16081 cgttagaaga aattgctgac ggtaaaattc gccctgttat ttcaagtgat tattatggta
    16141 aagaatagtt tcgaccatta aaaatatgtg aagctagacg tacatcaaat acgtcaaggc
    16201 atatccaaat caaacgcacc tatgtaggtg tgttttttta gtttatccaa tgattgatgt
    16261 tataataata ctaaatttgt atctataaaa aagtaatgag catttgtgcg catatgatga
    16321 tgtaaagcgt aaatgtcata gtgatattta agggagattg aatatgaaga aaatattatt
    16381 agccgttaca ggtggcattg cggcatataa agcaattgat ttgacaagta agttaacaca
    16441 atctgggtat gaagttcgcg ttatgttaac gaatcacgca caaaaatttg tgacaccatt
    16501 agcatttcaa gcaataagtc gaaatgctgt ttatacagat acttttatag aagaaaatcc
    16561 ttcagaaata cagcatattg cattaggtga ttgggcagat gcaatcattg ttgcacctgc
    16621 aacggcaaat acaattgcaa aattgagtgt aggtattgct gatgatttgg tgacatcaac
    16681 gttgctagca acagagacac cgaaatttat tgcgcctgct atgaatgtgc atatgtatga
    16741 aaataaacgt acgcagcaaa atattaatat tttaaaagaa gatgggtatc attttatcga
    16801 accaggaagc ggatttctag catgtggtta tgttgctaaa ggacgtatgg aagaaccgct
    16861 tcaaatcgtt tctgtaattg atgctcattt tcaaaatagt aatcgtttag ctaatagttc
    16921 atttcaagat aaacgcgcat tggttacagc aggaccaact attgaagtta tcgatccagt
    16981 cagatttgta tccaatcgtt cttctggaaa aatgggttat gcaatagctg aagcattgcg
    17041 aaatcgagga gctatcgtga cgttagttgc tggtcctaca acactagagg atccaaaaga
    17101 tattgaagtt attcatgttc aaagtgctga agaaatgttt gaacaagtga caagccgatt
    17161 tgacgaacaa gatattgttg taaaagcagc agccgtatct gactatacac cggttgatgt
    17221 attagaacat aagatgaaaa agcaggatgg tgatttgtca gtatctttta aacgtactaa
    17281 agacattctt aaatatttag gtgaacataa aacatcacag tatttaatag gctttgcagc
    17341 agagactgaa gatattgaaa attatgcaca acaaaaatta cgcaagaaaa atgcagatgt
    17401 gattatttca aataatgttg gggatatgtc tatcggattt agttctgatg ataatgaatt
    17461 gacaatgcat tttaaaaata atgaaaaggt aaatatcaag aaaggaaaaa aagtagtatt
    17521 agctgcacaa attttagatg aactagaaac taggtggcaa taatgatagc gaaagtcata
    17581 gtcgatgtcg cgtcgaagag cgttgactat aaatttgatt atataattcc cgaacaactc
    17641 gaatctgtca tccaacctgg tgtgcgtgta attgtacctt ttggaccaag aacgattcaa
    17701 ggttatgtaa tggaagtaac agcagaacct gatgcacaac ttgacgtttc gaagttaaaa
    17761 aaaatcatag aagtgaaaga tatacaacca gaattaacat cagaattaat agctttaagt
    17821 gagtggatgg gttcaactca tgtcattaaa cgtatttcta tgctagaagt gatgcttccg
    17881 agtgctatta aagcgaagta taaaaaagca tttaagatga aagatgacat agagctacct
    17941 tcagctttat tacaaaaatt tgataagcat ggttactatt attataaaga tgcgcaaaaa
    18001 aataatgata ttcaattgct tatgaagttg ttaaaagatg atatcgttga agaaaaaacg
    18061 attctcacac aaaatataac taaaaaaacc aagcgtgctg ttcgtgtcat tgaagggtat
    18121 catcctgatg aagtattagc taagttggag aaagttatta aacaatacga tttgtatgct
    18181 tacttgtctg aagaacaaca taaaacaata tttttaactg atattgagga tatgggcttt
    18241 tcaaaatcca gtttagatgg acttatcaaa aaaggttatg ttgaaaaata tgacgcggtt
    18301 gttgaaagag acccatttaa agatcgtgtt ttcgaacaag aatcaaaaca gcaattaaca
    18361 gaagaccaat ataaagcata tgaagcgatt aaagctaaaa ttgtaagcca agagcaagaa
    18421 acatttttac ttcatggtgt gacgggatca ggtaaaacag aagtatattt acaaacgata
    18481 gaagatgttt taagccaagg aaaacaggcg atgatgttag ttcctgaaat cgctctaaca
    18541 ccgcaaatgg ttttacgctt caaacgtcga tttggtgatg acgttgctgt attacattct
    18601 ggcttatcta atggggaacg ttatgatgag tggcaaaaaa ttagggatgg tcgtgcgaga
    18661 gtaagtgttg gtgcaaggtc aagtgtgttc gcacctttca aaaatttagg gttaatcatc
    18721 attgatgaag aacatgaatc tacatataaa caagaagatt atccgagata tcacgctaga
    18781 gaaattgccc aatggcgaag tgaatatcat cactgtccag tcattttagg aagtgcaaca
    18841 ccatgtcttg aaagttatgc acgagctgaa aaaggcgttt atcatttgct atcattacca
    18901 aacagagtga accaacaagc tttacctgaa attgatatag tagacatgcg tgaagaattg
    18961 agtgaaggta atcggtcaat gttttcaaaa gatttacgtg aagccataca attaagatta
    19021 gatcgacagg aacaagttgt tttattttta aatcgacgtg gttatgcatc gtttatgtta
    19081 tgtcgggatt gtggatatgt accgcaatgt ccaaactgtg atatttcatt aacgtatcat
    19141 aaaacgacag acttattaaa atgtcactat tgtggttacc aagagacgcc accgaatcaa
    19201 tgtccaaatt gtgagagtga acacattcga caagtaggta ctggtactca gaaagttgaa
    19261 gaactattgc aacaagaatt tgaagatgcg cgcataatta ggatggatgt agatacaacc
    19321 tcaaagaaag gtgcacatga aaagttattg actgaattcg aaaaaggtaa cggtgacatt
    19381 ttactaggta ctcagatgat tgcgaaagga ttagattatc caaatattac tttagttggt
    19441 gtgctgaatg cagatacaat gttaaattta cctgattttc gggcgagcga acgtacttat
    19501 caactattaa cgcaagtggc tggtagagct ggtcgtcatg aaaaggcagg tcaagtcatc
    19561 attcaaacgt ataatccaga tcattattca atattggatg ttcaaaaaaa tgattattta
    19621 acattttatc gtcaggaaat ggaatatcgt aaattaggaa agtatccacc gtattattat
    19681 ttgattaatt tcacaatctc acataaagaa atgaagaagg ttatggaagc atcgcagcat
    19741 gttcataaaa ttttattaca gcatttaaca gaaaaagcgc ttgtactagg tccatctccg
    19801 gcagcacttg cgagaatcaa caatgaattt agattccaaa ttttagtgaa atataaaagt
    19861 gaacctggat tattacaagc cattcagttt ttagatgact attaccatga aaaatttata
    19921 aaagaaaaat tagcattgaa gattgatatt gatccacaga tgatgatgta attttttgac
    19981 taaaacactt taatatcaat gtttcctaag agtatgaaag gtcttgtttt tactttaagt
    20041 acagaaaaag ggcagaatat acaactgtta accatttaaa ttagcagttt atattctgtc
    20101 tttttatatg gctttataac ttacgtgatt ttgatttgat taggaattta ttagtatttt
    20161 catttacgaa ttctgatttt aatgtaggtg ccatacttac tgatgaataa aagtactaat
    20221 gattaaacaa actattttta aatggcaaat tgaaatagtg caaattttac acttataaat
    20281 taatggctat aaactatttt atatctatct atgttttaac tacttaaatt attagacaaa
    20341 acaattttct taaattatca attaaag
//
LOCUS       DAMZKC010000035        19843 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.035, whole genome shotgun sequence.
ACCESSION   DAMZKC010000035 DAMZKC010000000
VERSION     DAMZKC010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 19843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19843
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.035"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..848
                     /locus_tag="POB55_000935"
     CDS             <1..848
                     /locus_tag="POB55_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499909.1"
                     /translation="FSNPNYRKILFEGSEYQQVSNNQGIFSKFAEMISTSLPYGVIFS
                     QIIILSMIAALIIYLLLRSDRYVHLTILKRRIIMIGFITLPLYYLLFYNQFLLNKNTD
                     IGLVGFVNVIVCGYFAFSIFVGIYLSINDRKTQITLYSLLIAICVSASTLVIVTPIEP
                     GNFLIVYTIHVIILIILLKEFRKYKSINVNFIKGASLVLAVIYLSAFIYVHYEHEKRV
                     QLLKQQIKDHPRQEVYTVESLPFEHYMHHPSPINKNYQEWFNNYEELPKMTKVKYIPF
                     GSDDS"
     gene            1142..3772
                     /gene="polA"
                     /locus_tag="POB55_000936"
     CDS             1142..3772
                     /gene="polA"
                     /locus_tag="POB55_000936"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HDI6499910.1"
                     /translation="MNKLVLIDGNSLSFRAFYALPLLSNKAGIHTNAVYGFAMLLEKI
                     LKEEKPNHFLVAFDAGKTTFRHEKYSEYKGGRQKTPPELSEQFPYIRQLLDAYHIKRY
                     ELDNYEADDIIGTLSKEADKAGFQTIIITGDRDLTQLATDNVTIYYTKKGVTDVDHYT
                     PDFIAEKYNGLTPNQIIDMKGLMGDTSDNIPGVAGVGEKTAIKLLNQFDTVEGVYEHL
                     DEISGKKLKEKLQNSKEDALMSKELATINVDSPIEVKLEDTLMTHQDEQQEKIELFKK
                     LEFKQLLADIDQSASVEDAIEKTFEIETSFDNIDFTSLKEAAIHFELDGGNYLRNNIL
                     KFSLFTGEKHIVINADDINNYVELVSWLENPNSKKVVYDAKKTYVASHRLGIDIQNIS
                     FDIMLASYIIDPSRTISDVQSVVSLYGQSFVKDDVSIYGKGKKFKVPEDDVLNPYVAS
                     ITDAIYFAKPNMDKQLEEYNQVELLADLELPLAKILSEMEEIGIFTDVHDLEEMEKEI
                     QEKLDVLIRNIHDAAGEDFNINSPKQLGVVLFETLQLPVIKKTKTGYSTAVDVLEQLQ
                     GEHPIIDYILEYRQLSKLQSTYVEGLQKVISDDQRIHTRFNQTLAQTGRLSSVDPNLQ
                     NIPVRLEEGRKIRKAFKPTSKDSVILSADYSQIELRVLAHITQDESMKEAFINGDDIH
                     TATAMKVFGVEADQVDSLMRRQAKAVNFGIVYGISDYGLSQSLGITRKKAKAFIDDYL
                     ASFPGVKQYMSDIVKDAKALGYVETLLHRRRYIPDITSRNFNLRGFAERTAMNTPIQG
                     SAADIIKLAMVKFAQKMKETTYQAKLLLQVHDELIFEVPKSEVDSFSEFVEEIMENAL
                     QLDVPLKVDSSYGATWYDAK"
     gene            3788..4660
                     /gene="mutM"
                     /locus_tag="POB55_000937"
     CDS             3788..4660
                     /gene="mutM"
                     /locus_tag="POB55_000937"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HDI6499911.1"
                     /translation="MPELPEVEHVKRGIEPYVINQKIEHVIFSDKVIEGKAQGKETII
                     KGIELDTFKTLSEGYTITNVERRSKYIVFQLDNKREQRTLISHLGMAGGFFIVDELED
                     IMIPNYRKHWHVIFELSNDKKLIYSDIRRFGEIRNVASVASYPSFLEIAPEPFSNEAL
                     TYYLNRIHQQSNKNKPIKQVILDHKVIAGCGNIYACEALFRAGVLPDKKVKDLTHQQQ
                     EMVFYYVREVLEEGIKYGGTSISDYRHADGKTGEMQLHLNVYKQPVCKVCGSQIETKI
                     IATRNSHYCPVCQK"
     gene            4676..5299
                     /locus_tag="POB55_000938"
     CDS             4676..5299
                     /locus_tag="POB55_000938"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HDI6499912.1"
                     /translation="MPKVIGLTGGIASGKSTVSELLSVFGFKVVDADKAAREAVKKGS
                     KGLAQVREVFGDEAIDENGEMNRRYMGDLVFNHPEKRLELNAIIHPIVRDIMEEEKQE
                     YLKQGYNVIMDIPLLFENELENTVDEVWVVYTSESIQMDRLMQRNNLSLEDAKARVYS
                     QISIDKKSRMADHVIDNLGDKLELKQNLERLLEEEGYIEKPNYGEED"
     gene            5469..6494
                     /gene="gap"
                     /locus_tag="POB55_000939"
     CDS             5469..6494
                     /gene="gap"
                     /locus_tag="POB55_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDI6499913.1"
                     /translation="MSTNIAINGMGRIGRMVLRIALQNKNLNVVAINASYPPETIAHL
                     INYDTTHGKYNLKVEPIENGLQVGDHKIKLVADRNPENLPWKELDIDIAIDATGKFNH
                     GDKAIAHIKAGAKKVLLTGPSKGGHVQMVVKGVNDNQLDIEAFDIFSNASCTTNCIGP
                     VAKVLNNQFGIVNGLMTTVHAITNDQKNIDNPHKDLRRARSCNESIIPTSTGAAKALK
                     EVLPELEGKLHGMALRVPTKNVSLVDLVVDLEKEVTAEEVNQAFENAGLEGIIEVEHQ
                     PLVSVDFNTNPNSAIIDAKSTMVMSGNKVKVIAWYDNEWGYSNRVVDVAEQIGALLTS
                     KETVSAS"
     gene            6705..7175
                     /gene="nrdR"
                     /locus_tag="POB55_000940"
     CDS             6705..7175
                     /gene="nrdR"
                     /locus_tag="POB55_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HDI6499914.1"
                     /translation="MKCPKCNSTQSKVVDSRHADELNAIRRRRECENCGTRFTTFEHI
                     EVSQLIVVKKDGTREQFSREKILNGLVRSCEKRPVRYQQLEDITNKVEWQLRDEGHTE
                     VSSRDIGEHVMNLLMHVDQVSYVRFASVYKEFKDVDQLLASMQGILSENKRSDA"
     gene            7176..8576
                     /locus_tag="POB55_000941"
     CDS             7176..8576
                     /locus_tag="POB55_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HDI6499915.1"
                     /translation="MGRQAFEFGLRPKDQFKVMQHFDLNTNHLEVLNRLYTPLIGTQA
                     VGLYHFMTQFVKESHNETLILSHYIFMNELKINLLEFRQQMDLLEAIGLLKAFVKHDE
                     QETQFVYQLIQPPSAHLFFNDPMLSIFLYSEVEHRRFHELKKYFEYQQIDLSEFKQVT
                     RQFTDVFKVPSTKIDIDTSDIPINEPYQGIDLSNESFDFEMLRQMLGKHFISQDIVTK
                     DAKRLITQLATLYGLTADGMKHVILNSITSGQQLSFEEMRKQARSYYLMEHENQMPKL
                     QVKSPATSSSTGKSSEVNPKPQSDEWFELLEQTSPIDMLASWSESEPTISQKAMVEEL
                     IEREKMSFGVINILLQFVMLKEDMKLPKAYILEIASNWKKKGIKTAKEAYNYAKKVNQ
                     PKNEGSSGNYQKRGSYFGQRNRISKEKTPKWLENRDKPSEEDSAKDNSVDDQQLEQDR
                     QAFLDKLSKKWEEDSQ"
     gene            8576..9496
                     /gene="dnaI"
                     /locus_tag="POB55_000942"
     CDS             8576..9496
                     /gene="dnaI"
                     /locus_tag="POB55_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HDI6499916.1"
                     /translation="MKQFKSIINTSQDFEKRIEKIKKEVINDPDVKQFLEAHRAELTN
                     AMIDEDLNVLQEYKDQQKHYDGHKFADCPNFVKGHAPELYVDNNRIKIRYLQCPCKIK
                     YDEERFEAELITSHHMQRDTLNAKLKDIYMNQRDRLDVAMAADDICTAITNGEQVKGL
                     YLYGPFGTGKSFILGAIANQLKSKKVRSTIIYLPEFIRTLKGGFKDGSFEKKLHRVRE
                     ANILMLDDIGAEEVTPWVRDEVIGPLLHYRMVHELPTFFSSNFDYSELEHHLAMTRDG
                     EEKTKAARIIERVKSLSTPYFLSGENFRNN"
     gene            9589..9711
                     /locus_tag="POB55_000943"
     CDS             9589..9711
                     /locus_tag="POB55_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499917.1"
                     /translation="MFDENMVNKLDDYYFIYGICNEYPDQDRYLKQRYLIHKLY"
     gene            9908..11845
                     /gene="thrS"
                     /locus_tag="POB55_000944"
     CDS             9908..11845
                     /gene="thrS"
                     /locus_tag="POB55_000944"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HDI6499918.1"
                     /translation="MEQINIQFPDGNKKAFDKGTTTEDIAQSISPGLRKKAVAGKFNG
                     QLVDLTKPLETDGSIEIVTPGSEEALEVLRHSTAHLMAHAIKRLYGNVKFGVGPVIEG
                     GFYYDFDIDQNISSDDFEQIEKTMKQIVNENMKIERKVVSRDEAKELFSNDEYKLELI
                     DAIPEDENVTLYSQGDFTDLCRGVHVPSTAKIKEFKLLSTAGAYWRGDSNNKMLQRIY
                     GTAFFDKKELKAHLQMLEERKERDHRKIGKELELFTNSQLVGAGLPLWLPNGATIRRE
                     IERYIVDKEVSMGYDHVYTPVLANVDLYKTSGHWDHYQEDMFPPMQLDETESMVLRPM
                     NCPHHMMIYANKPHSYRELPIRIAELGTMHRYEASGAVSGLQRVRGMTLNDSHIFVRP
                     DQIKEEFKRVVNMIIDVYKDFGFEDYSFRLSYRDPEDKEKYFDDDDMWNKAENMLKEA
                     ADELGLSYEEAIGEAAFYGPKLDVQVKTAMGKEETLSTAQLDFLLPERFDLTYIGQDG
                     EHHRPVVIHRGVVSTMERFVAFLTEETKGAFPTWLAPKQVQIIPVNVDLHYDYARQLQ
                     DELKSQGVRVSIDDRNEKMGYKIREAQMQKIPYQIVVGDKEVENNQVNVRQYGSQDQE
                     TVEKDEFIWNLVDEIRLKKHR"
     gene            12271..13764
                     /locus_tag="POB55_000945"
     CDS             12271..13764
                     /locus_tag="POB55_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDI6499919.1"
                     /translation="MSKVQNESNNVVKRGLKDRHISMIAIGGCIGTGLFVTSGGAIHD
                     AGALGALIGYAIIGIMVFFLMTSLGEMATYLPVSGSFSTYATRFVDPSLGFALGWNYW
                     FNWVVTVAADITIAAQVIQYWTPLQGIPAWAWSALFLVIIFSLNSLSVRVYGESEYWL
                     ALIKVVTVIVFIAIGLLTIVGIMGGHVVGFEIFNKGEGPILGGNLGGSLLSILGVFLI
                     AGFSFQGTELIGITAGESENPERAVPKAIKQVFWRILLFYILAIFVIGMLIPYDSSAL
                     MGGSDNVATSPFTLVFKNAGFAFAASFMNAVILTSVLSAGNSGMYASTRMLYSMSKDK
                     LAFEAFGKTNKNGVPYMSLLVTAIIVVIIFVLQSLTKGAYEYIVAASGLTGFIAWVGI
                     AVSHYRFRRAFDKQNYDKSKLKYTAKLFPFGPLFAGFLCIVVIIGQDVDFIKTGDFNF
                     SRFLITYMGIPVFLAFFIYHKLRYKTKKIPLEQVDLRQDVSMDEIKG"
     misc_feature    13839..13964
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            13993..14520
                     /gene="infC"
                     /locus_tag="POB55_000946"
     CDS             13993..14520
                     /gene="infC"
                     /locus_tag="POB55_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HDI6499920.1"
                     /translation="MSTIAKDQTQINDKIRAKELRLIGQDGEQIGVKSKREALEMAER
                     VDLDLVVVAPNAKPPVARIMDYGKFKFEQQKKEKEMKKKQKIINVKEIRLSPTIEEHD
                     FQTKLKNGRKFLTKGDKCKVSIRFRGRAITHKEIGQRVLEKYADECKDIATVEQKPKM
                     DGRQMFIMLAPTAEK"
     gene            14549..14749
                     /gene="rpmI"
                     /locus_tag="POB55_000947"
     CDS             14549..14749
                     /gene="rpmI"
                     /locus_tag="POB55_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HDI6499921.1"
                     /translation="MPKMKTHRGAAKRVKRTASGQLKRSRAFTSHLFANKSTKQKRQL
                     RKARLVSKSDMKRVKQLLAYKK"
     gene            14796..15152
                     /gene="rplT"
                     /locus_tag="POB55_000948"
     CDS             14796..15152
                     /gene="rplT"
                     /locus_tag="POB55_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HDI6499922.1"
                     /translation="MPRVKGGTVTRARRKKTIKLAKGYFGSKHTLYKVAKQQVMKSGQ
                     YAFRDRRQRKRDFRKLWITRINAAARQHEMSYSRLMNGLKKAGIDINRKMLSEIAISD
                     EKAFAQLVTKAKDALK"
     gene            15294..15902
                     /locus_tag="POB55_000949"
     CDS             15294..15902
                     /locus_tag="POB55_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HDI6499923.1"
                     /translation="MSKFDEQIIVVPREIIFNNEKNNFNGFLNKNKPEGQNIFDALSQ
                     YEVKRRGDMEEDPSYKQLISYCLLENEHGEILVYERLSGGGEARLHGQSSIGVGGHMN
                     DVPGAESINEVLRVNAQRELEEEVGLSEQDSQNMEYIGFINDDNNEVGKVHIGVVFKI
                     TVSTNDVEAKETDTLRIKWVEKGNIESYDDFETWSALILQDL"
     gene            15921..16850
                     /locus_tag="POB55_000950"
     CDS             15921..16850
                     /locus_tag="POB55_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499924.1"
                     /translation="MSDIIQFPNSSKKLYKDIKRAEQDQNYDLMYEYIVQYERQFELT
                     EEIAMMKCRMLYETGSFLELREETIVLLKTGIQQYDALMIYYVKSLIGLGQYFEAVEV
                     IHQIIDEVKDHKTRMALHPLKEFAKSKLIEDEKRLTQSLTDFDTLSMREQTHLILKLI
                     DNGHFQFQETVLYILKSNTYSYNLISLMIEYLRFANCTQELTIEKYGMDVTFVPANLN
                     GLEHTTLKEKVIPNVMETLNDGALHIAEEAHHIMNNHSIMIYPIDIETLFETNKWINA
                     YECYFKNMLGLKCELQSIDAFNFIQQLDLNNNS"
     gene            16855..16965
                     /locus_tag="POB55_000951"
     CDS             16855..16965
                     /locus_tag="POB55_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499925.1"
                     /translation="MQNNTQHFNIVLTKKVDLQIYKRCDFNVCIGNRPMI"
     gene            17013..18314
                     /locus_tag="POB55_000952"
     CDS             17013..18314
                     /locus_tag="POB55_000952"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HDI6499926.1"
                     /translation="MTATWEKKEGNEGLLTVTVPAEKVNKALDQAFKKVVKQINVPGF
                     RKGKVPRPIFEQRFGVEALYQDAIDILLPDAYGEAIDETDIKPVAQPEVSVTQIEKGK
                     DFIFEATVTVEPEVKLGDYKGLEIEKQETELSDDELQEAIDHSLGHLAEMVVKEDGVV
                     ENGDTVNIDFSGSVDGEEFEGGQAEGYDLEIGSGSFIPGFEEQLEGMKVDEEKDVVVT
                     FPEEYHAEELAGKEATFKTKVNEIKFKEVPELTDEIANELDAEANTVDEYKENLRKRL
                     AEQKATDAENVEKEEAITKATDNTTIDIPEAMVNTELDRMVSEFAQRIQQQGLDLQTY
                     FQISGQDETQLREQMKDDAEQRVKTNLTLTAIAEAEKIEATDEDIDKELEKMSKQFNI
                     SVEDIKNTLGNTDIIKNDVRIQKVIDLLRDNAKFVEGTKED"
     gene            18465..19727
                     /gene="clpX"
                     /locus_tag="POB55_000953"
     CDS             18465..19727
                     /gene="clpX"
                     /locus_tag="POB55_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HDI6499927.1"
                     /translation="MFKFNEDEENLKCSFCGKDQDQVKKLVAGSGVYICNECIELCSE
                     IVEEELAQNTSEAMTELPTPKEIMDHLNEYVIGQEKAKKSLAVAVYNHYKRIQQLGPK
                     EDDVELQKSNIALIGPTGSGKTLLAQTLAKTLNVPFAIADATSLTEAGYVGDDVENIL
                     LRLIQAADFDIDKAEKGIIYVDEIDKIARKSENTSITRDVSGEGVQQALLKILEGTTA
                     SVPPQGGRKHPNQEMIQIDTTNILFILGGAFDGIEEVIKRRLGEKVIGFSSNEADKYD
                     EQALLAQIRPEDLQAYGLIPEFIGRVPIVANLETLDVTALKNILTQPKNALVKQYTKM
                     LELDNVDLEFTEEALSAISEKAIERKTGARGLRSIIEESLIDIMFDVPSNENVTKVVI
                     TAQTINEETEPELYDAEGNLINNSKTSA"
BASE COUNT         7302 a         2684 c         3797 g         6060 t
ORIGIN      
        1 tgttttccaa tccaaattat cgcaaaattt tatttgaagg atcagaatat caacaagttt
       61 caaataatca aggtatcttt tcaaaatttg cagaaatgat atcaacgtca cttccatatg
      121 gtgtcatttt cagtcagatt ataattttaa gtatgattgc agcgcttatt atttatctac
      181 tgcttagaag tgatcggtat gtacatttaa caatattaaa gcgtcgtatc attatgatag
      241 gatttatcac tttaccgctt tattatttac tattttacaa tcagttttta ttaaataaaa
      301 atacagatat tggattggtt ggttttgtga acgtaattgt atgtggttac tttgcgtttt
      361 cgatatttgt aggtatttat ttgtctatta atgatcgtaa aacacaaatc actttatatt
      421 ccttacttat tgcaatatgt gtttcggcgt cgacactagt cattgttact ccaatagaac
      481 caggtaattt tttgattgtt tatacgatac atgtaatcat tttaattatt ttattaaaag
      541 aatttcggaa atataagtca atcaatgtta attttattaa aggtgcatca ctagttttag
      601 ctgtgatata tcttagtgca tttatttatg ttcattatga acatgaaaaa agagttcagt
      661 tattaaaaca acaaattaaa gatcatccac gacaagaggt ttatacagtg gaatcattgc
      721 cgtttgaaca ttatatgcat catccgtcac caatcaataa aaattaccaa gagtggttta
      781 ataattatga agaattaccg aaaatgacca aagttaaata tattccattt ggttcagacg
      841 attcataact tattcttacc tataaaaatt taaaatagca gcataaaaat attaaaaagt
      901 agacataatc atgaaatgat aatgtgcatc tcaaaagttg gacttttaac ctgactttat
      961 ggagtgctca tcaagaacaa tcatgattat gtctttttta ttttttagct aagtctatat
     1021 aggcacttgt tttatttgaa attaggtcgt gtataaatac tctatgagat agtttcttta
     1081 attacgacat cacagtcgaa tatgctaaaa tatatataaa ttagttctgg aggttaaaat
     1141 tgtgaataaa ttagtattaa tcgatggtaa tagcttaagt tttagagctt tttatgcatt
     1201 acctttgtta tcaaacaaag caggcattca taccaatgca gtatatggtt ttgcaatgtt
     1261 actagagaag attttaaaag aagaaaagcc aaatcatttt ttagtagcgt ttgatgcagg
     1321 taaaacgact ttcagacatg aaaaatatag tgaatataaa ggtggacgcc agaaaacgcc
     1381 gcctgaacta agtgagcaat ttccttatat tcgccaatta ttagatgctt atcatattaa
     1441 acgttatgaa ttagataact atgaggcaga tgatattatc ggaactttaa gtaaagaagc
     1501 agataaagcg ggatttcaga caattattat tacgggagac cgagatttaa cacaacttgc
     1561 aacggataat gtaaccattt attacactaa aaaaggtgtt actgatgttg atcattatac
     1621 acctgacttt attgcggaaa aatacaacgg gttaacacct aatcaaatta ttgatatgaa
     1681 aggattaatg ggagatactt ctgataatat accaggtgtt gcaggtgttg gagagaaaac
     1741 agcaataaaa ttgctaaacc aatttgacac agtagaaggt gtctatgaac atttagatga
     1801 aatttcaggt aaaaagttaa aagaaaaact tcaaaatagc aaagaagatg cattaatgag
     1861 taaagaatta gcaacgatta atgtagatag tccgattgaa gtaaaacttg aagatacgtt
     1921 aatgactcat caagatgaac aacaagaaaa aatcgaattg tttaagaagt tagaattcaa
     1981 acaattgttg gctgacattg atcaatcagc aagcgttgaa gatgcaatag aaaagacatt
     2041 tgaaattgaa acgtcttttg ataatattga ttttacttca ttgaaagaag cggccatcca
     2101 tttcgaatta gacggtggta actatttgcg aaataatatt ttaaaattct ctttatttac
     2161 aggtgagaaa catattgtaa ttaatgcgga tgacataaat aattatgtcg aactagtttc
     2221 atggttagaa aatccgaatt cgaaaaaagt cgtatatgat gctaaaaaaa catatgtagc
     2281 atcacataga ttggggattg atattcaaaa tatttctttc gatattatgt tggccagtta
     2341 tataattgat ccatctcgta cgattagtga tgttcaatca gttgtttcat tgtacggtca
     2401 aagttttgtg aaagacgatg tgagtatata tggaaaaggt aagaaattta aggtacctga
     2461 agatgatgtt ttaaatccat atgttgcttc tattactgat gcaatttact ttgcgaaacc
     2521 aaatatggat aaacaattag aagaatacaa tcaggtagaa ctcttggctg atttagagct
     2581 accgctagct aagattttaa gtgaaatgga agaaattggt atatttacag atgttcatga
     2641 tttagaagaa atggaaaaag aaattcaaga aaaattagac gtcttgattc gaaatatcca
     2701 tgatgcagct ggtgaagatt ttaatataaa ttctcctaag caattaggtg ttgtattgtt
     2761 tgagacatta caattacctg ttattaaaaa gacgaaaaca ggatattcta ctgctgtaga
     2821 tgtcttagag caattgcaag gtgaacatcc tatcattgat tatattttag aatatcgtca
     2881 attatctaag ttacagtcta cttatgttga aggattgcaa aaggtaatta gtgatgatca
     2941 acgtatccat acacgtttta atcaaacttt agcgcaaact ggacgtttat caagtgtaga
     3001 tcctaattta caaaatattc cggttagact tgaagaaggg cgtaaaataa gaaaagcctt
     3061 taaaccaact tcaaaagata gcgttatatt atcagcagat tattctcaaa ttgaattgcg
     3121 cgtattagca cacattacac aagatgagag tatgaaagaa gcatttatca acggcgatga
     3181 tattcataca gcaactgcta tgaaagtatt tggtgtagaa gctgatcaag tcgatagttt
     3241 aatgcgtcgt caagcaaaag cagttaactt tggaattgtt tatgggataa gtgattatgg
     3301 tttaagtcaa agtttaggta ttactcgtaa aaaagcaaaa gcattcattg atgattattt
     3361 agctagtttc ccaggtgtaa aacaatatat gtctgatatt gtaaaagatg ccaaagcttt
     3421 aggttacgtg gaaacattgc tacatcgtcg acgctatatt cctgatatta cgagtcgtaa
     3481 ctttaattta cgcggctttg ctgaacgtac tgctatgaat acgccaatac agggcagtgc
     3541 tgcagatatc attaaactgg caatggttaa atttgctcaa aaaatgaaag agacaacata
     3601 tcaagctaaa ctattattac aagtacacga tgaattaatt tttgaagtac ctaagtcaga
     3661 agtagattca tttagtgaat ttgtagaaga gataatggaa aatgcattgc aattagatgt
     3721 tccattaaaa gtagactcaa gttatggtgc aacttggtat gatgcaaaat aaagtgaggt
     3781 taatggtatg cccgaattac cagaagtaga acatgtaaaa agaggaattg aaccctacgt
     3841 tataaatcaa aaaattgaac acgttatatt ttcggataaa gtaatcgaag gtaaagctca
     3901 aggcaaggaa acaattataa aaggaattga attagatact tttaaaactt tatcggaagg
     3961 ctataccatt acaaatgtag aacgaagaag taaatatatc gtttttcagc tagataataa
     4021 acgagaacaa cgtacattaa ttagtcattt aggtatggcg ggcggttttt tcattgtaga
     4081 tgaacttgaa gatattatga tacctaatta tcgtaagcat tggcatgtta tttttgagtt
     4141 gtcaaatgac aaaaagttaa tttattcaga tatacgtcgt tttggagaaa ttagaaatgt
     4201 tgcgtctgta gcatcttatc cttcattttt agaaatagcg cctgaacctt tttcaaatga
     4261 agcattgacg tattatttaa atcgaattca tcaacagtct aataagaata aaccaatcaa
     4321 acaagtgatt ttggatcata aagtaatcgc aggttgtgga aatatttacg catgtgaagc
     4381 gttgtttcgt gcaggtgttt taccagataa aaaagtaaaa gacttaacac accaacaaca
     4441 agaaatggtt ttttattatg ttagagaagt actagaagaa ggtattaaat atggcggtac
     4501 aagtatttca gattatcgtc atgctgatgg aaaaactggt gagatgcaat tgcatcttaa
     4561 tgtatataag cagccggtat gcaaagtgtg tggaagtcaa attgaaacaa aaatcatcgc
     4621 gacaagaaat agtcattatt gtccagtatg tcaaaaataa agaaagagtg aaattatgcc
     4681 gaaagttatt ggtctaacag gtggaatcgc ctcaggaaaa tcaacagtat cagaactctt
     4741 atccgtattc ggttttaaag tagtagatgc tgataaagca gccagggaag ctgttaaaaa
     4801 ggggagtaaa ggtttagctc aagtacgaga agtctttggt gatgaagcaa ttgatgaaaa
     4861 tggtgagatg aatcgtcgtt atatgggtga tctagtgttt aatcatccag aaaaacgctt
     4921 agaattaaat gctatcatac atcctatcgt gcgagatatt atggaagaag aaaagcaaga
     4981 atatttaaaa caaggatata atgtaatcat ggatattcca ttattatttg aaaatgaatt
     5041 ggaaaataca gtagacgaag tgtgggttgt atacacttct gaaagtatac aaatggatcg
     5101 tttaatgcaa cgtaataatt tgtcattaga agatgcgaaa gcacgtgtct atagccaaat
     5161 ttctattgat aaaaaaagcc gaatggccga tcatgttatc gataatttag gggataaact
     5221 tgaattaaaa caaaaccttg agagattgtt agaagaagaa ggttatattg aaaagccgaa
     5281 ttacggagaa gaagattaat attacactat aaataagtca ttactttacg tacgcgttga
     5341 tgtatgtaag taatgactat tttttataaa aaagataaat aaatcaacgg aaaacgcttt
     5401 caaatttcat ataatatgct atactaattc cataaagtat aacacataaa gatcaagggg
     5461 tgcttttaat gtcaacgaat attgcaatta atggtatggg tagaattgga agaatggtat
     5521 tacgtattgc attacaaaat aaaaatttaa atgtagtagc gataaatgct agttatccac
     5581 ccgaaacaat tgcacattta atcaattacg atacgacaca tggaaaatat aatctaaaag
     5641 ttgaaccgat tgaaaatgga ttgcaagttg gagatcataa aattaaattg gttgctgatc
     5701 gcaatcctga aaacttgcca tggaaagaat tagatatcga tattgctata gatgcaactg
     5761 gtaaatttaa tcatggtgat aaagccatcg cacatattaa agcaggtgcc aaaaaagttt
     5821 tgttaactgg tccttcaaaa ggtggacatg ttcaaatggt agttaaaggc gtaaatgata
     5881 accaattaga tatagaagca tttgacattt ttagtaatgc ttcatgtact actaattgca
     5941 ttggtccagt tgcaaaagtt ttaaataatc agtttggaat agttaatggt ttaatgacta
     6001 ctgttcacgc tattacaaat gaccaaaaaa atattgataa tccacataaa gatttaagac
     6061 gtgcacgttc atgtaatgaa agcattattc ctacttctac tggtgcggcg aaagctttaa
     6121 aagaagtatt accagaatta gaaggtaaat tacacggcat ggcattacgt gtaccaacaa
     6181 agaatgtatc gctcgttgat ttagttgttg atttagaaaa agaagtaact gcagaagaag
     6241 taaaccaagc ttttgaaaat gcaggtttag aaggtatcat agaagtcgaa catcaaccac
     6301 tagtgtctgt tgattttaat actaatccaa attcagctat tattgatgca aaatcaacaa
     6361 tggtcatgtc agggaataaa gtaaaagtta ttgcttggta tgataatgaa tggggttatt
     6421 caaatagagt tgtagatgtt gctgagcaaa ttggagcact tttgacatca aaagaaactg
     6481 taagtgcaag ttaatacaat taatttatag cctaactctg acttcggagt taggtttttt
     6541 attttttaat acaataatat tgtcatgcaa attacttaat aagtaacgat ataatgtttt
     6601 ttattgtttc attatttggt taaatgaatt aaattgaggg attctttttt catctctaga
     6661 tttatagtat aattaggcat aatcaaaata aagagggtga tgtaatgaaa tgcccgaaat
     6721 gtaattctac acaatctaaa gttgtagatt caaggcatgc cgatgaatta aatgccattc
     6781 gaagacgaag agaatgtgaa aattgtggaa cacgtttcac tacatttgaa catatcgaag
     6841 ttagtcagct tatagttgtg aaaaaagatg gcacaagaga gcagttttca agagaaaaga
     6901 tacttaatgg acttgtgcgt tcttgtgaga aacgaccagt tagatatcaa caacttgaag
     6961 acataacaaa caaggttgaa tggcaattac gagatgaagg tcatacggaa gtgtcttcac
     7021 gagatatagg tgaacacgtt atgaacttgt taatgcatgt tgatcaagtt tcatatgtta
     7081 gatttgcatc agtctataaa gaatttaaag atgttgatca attgttagca tcgatgcaag
     7141 ggattttaag cgaaaacaaa cggagtgatg cttaaatggg acgacaagcc ttcgaattcg
     7201 gcttaagacc aaaggatcaa ttcaaagtta tgcaacactt tgatttaaat acgaatcatt
     7261 tagaggtatt gaatcggctg tatacaccgc taattggaac acaagcagtg ggtttatacc
     7321 attttatgac tcaattcgtt aaagaaagtc ataatgaaac attgattctt tctcattata
     7381 tttttatgaa tgaacttaaa attaatttac tagagtttag acaacaaatg gacttacttg
     7441 aagcgattgg attgttaaag gcgtttgtca aacatgatga acaagaaaca caatttgttt
     7501 accaattaat tcaaccgcct tcagcacatt tattttttaa tgatccaatg ttgtcaatct
     7561 ttttatatag tgaagtcgag catcgtcgat ttcatgagtt gaagaaatac tttgagtatc
     7621 aacaaattga tttgagtgaa tttaaacagg taacaagaca atttactgat gtttttaaag
     7681 tgccgtctac aaaaatagat atcgatacaa gtgatattcc gattaatgaa ccgtaccaag
     7741 gtatcgattt atcaaatgaa tcttttgact ttgaaatgtt gagacaaatg ttaggtaaac
     7801 attttattag tcaagacatt gttaccaaag atgcaaagcg tttaataaca caactcgcga
     7861 ctttatatgg tttaacagca gatggcatga agcatgttat tttaaattca attacaagtg
     7921 gacaacagtt atcctttgaa gaaatgcgta aacaagcacg ctcgtattac ttaatggaac
     7981 acgaaaatca aatgcctaaa ttacaggtga aatctcctgc tacatcatct tcgacgggca
     8041 aatcttcaga agtgaatcca aagccacaaa gtgatgaatg gtttgagtta cttgaacaaa
     8101 cgagtccaat tgatatgtta gcttcttggt ctgaatcaga accaactata tcgcaaaagg
     8161 caatggttga agaattaatt gaacgtgaaa aaatgtcttt tggcgtcatt aatattttgt
     8221 tgcaatttgt catgttaaaa gaagatatga aacttcctaa agcgtatatt ttagaaatag
     8281 catcaaattg gaagaaaaaa ggtattaaga cagctaaaga agcatataat tacgctaaaa
     8341 aagttaatca acctaaaaat gagggttcta gcggaaatta ccaaaagcgt ggttcatact
     8401 ttgggcagcg taatcgtatt tcaaaagaaa aaacacctaa atggttagaa aatagagata
     8461 aacctagtga agaagattcg gctaaagata atagcgtaga tgatcaacaa ttagagcaag
     8521 atcgacaagc atttctagat aaattatcta aaaaatggga ggaggacagt caataatgaa
     8581 gcaatttaaa agtataatta acacgtcgca ggactttgaa aaaagaatag aaaagataaa
     8641 aaaagaagta atcaatgacc cagatgttaa gcaatttttg gaagcgcatc gagctgaatt
     8701 aacgaatgct atgattgatg aagacttaaa tgtgttacaa gagtataaag atcaacaaaa
     8761 acattatgac ggtcataaat ttgctgattg tccaaatttc gtaaaggggc atgcgcctga
     8821 gttatatgtt gataataacc gaattaaaat acgctattta caatgcccat gtaaaatcaa
     8881 gtacgacgaa gaacgctttg aagctgagct aattacatct catcatatgc aacgagatac
     8941 tttaaatgcc aaattgaaag atatttatat gaatcaacga gaccgtcttg atgtagctat
     9001 ggcagcagat gatatttgta cagcaataac taatggggaa caagtgaaag gcctttacct
     9061 ttatggtcca tttgggacag gtaaatcttt tattctaggt gcaattgcga atcagctcaa
     9121 atctaagaag gtacgttcga caattattta tttaccggaa tttattagaa cattaaaagg
     9181 tggctttaaa gatggttctt ttgaaaagaa attacatcgc gtaagagaag caaacatttt
     9241 aatgcttgat gatattgggg ctgaagaagt gactccatgg gtgagagatg aggtaattgg
     9301 acctttgcta cattatcgaa tggttcatga attaccaaca ttctttagtt ctaattttga
     9361 ctatagtgaa ttggaacatc atttagcgat gactcgtgat ggtgaagaga agactaaagc
     9421 agcacgtatt attgaacgtg tcaaatcttt gtcaacacca tattttttat caggagaaaa
     9481 tttcagaaac aattgaattt taaaatgatt ggtgtataat gaatacaaat ctaaatcgtt
     9541 taaatgattg aagacaagat gatctaatca atattacaca gaaagccatt gtttgatgag
     9601 aatatggtta ataaattaga tgattactac ttcatttatg gtatttgtaa tgaatacccg
     9661 gatcaagacc gttatcttaa gcagagatat ttaatacata aattgtatta aataactagg
     9721 gtggtaccac gacgatctcg tcccttgtat aggggcggga ttttttgttt tttcagacat
     9781 aaatgtttgt tggtgtcata aattccctgt ttattgttaa taggtttaat gttaaaacga
     9841 tgattgttgt tcaatttttt aacgaggtca gataaaagta tttataaagc aaataggagg
     9901 gtttaacatg gaacaaatta atattcaatt tccagatggt aataaaaagg cgtttgataa
     9961 aggtactact actgaagata tagcacaatc aattagtcct ggattacgta aaaaagctgt
    10021 tgccggcaaa tttaacgggc aacttgtaga tttaacaaaa ccgcttgaaa ctgatggatc
    10081 aattgaaatt gtgacaccag gtagtgaaga agcgttagag gtattacgtc attctactgc
    10141 acatttaatg gcacacgcga ttaaaaggtt atatggtaat gttaaatttg gtgtaggtcc
    10201 tgtaatagaa ggtggattct actatgactt cgacattgac caaaacatct catctgatga
    10261 ctttgaacaa attgaaaaga caatgaaaca aatcgttaac gaaaatatga aaatcgaacg
    10321 aaaagtggtt tcacgagatg aagcgaaaga gttattcagc aatgatgaat acaaattaga
    10381 attaatcgac gcgattcctg aagatgaaaa tgtaacatta tatagtcaag gtgattttac
    10441 tgatttatgt cgtggagttc acgttccatc aacagctaaa attaaagagt ttaaactatt
    10501 atctacagca ggtgcatact ggcgtggaga tagtaacaac aaaatgttac aacgtatata
    10561 cggtactgct ttctttgata aaaaagaatt aaaagcacat ttacaaatgt tagaagagcg
    10621 taaagaacgt gatcatcgta aaattggtaa agagttagaa ttattcacaa atagccaatt
    10681 agttggtgct ggtttgccat tatggttacc taacggtgca acaattagac gtgaaattga
    10741 acgttacatt gttgataaag aggtgagcat ggggtatgac cacgtttata caccagtact
    10801 tgctaatgtt gatttataca aaacatctgg tcactgggat cactatcaag aagatatgtt
    10861 cccaccaatg cagttagatg aaactgaatc tatggtatta cgtccaatga actgtccaca
    10921 tcatatgatg atttatgcga ataaaccaca ttcatatcgt gaattaccta tccgtatcgc
    10981 tgagctagga acgatgcata gatatgaagc aagtggtgct gtatcaggat tacaacgtgt
    11041 tcgtggtatg actttaaatg attcacatat ctttgttcga cctgatcaaa ttaaagaaga
    11101 attcaaacgc gttgtaaaca tgattattga tgtgtataaa gactttggtt tcgaggatta
    11161 tagctttaga ttaagttata gagaccctga agataaagaa aagtactttg atgatgatga
    11221 tatgtggaat aaagctgaaa atatgcttaa agaggcagcg gatgagcttg gcttatcgta
    11281 cgaagaagcg attggtgaag cggcattcta tggtccgaaa ctagatgttc aagttaaaac
    11341 agcgatgggt aaagaagaga cattatcaac agcacaactt gatttcttat taccagaacg
    11401 ttttgattta acttatattg gtcaagatgg tgaacatcat cgtccagttg ttattcatcg
    11461 tggtgttgta tcaacaatgg aacgatttgt agcattttta actgaagaaa ctaaaggtgc
    11521 attcccaact tggttagcgc caaaacaagt tcaaatcatt ccagttaacg ttgatttaca
    11581 ttatgattat gcgcgccaat tacaagatga attgaaatct caaggcgttc gtgtaagtat
    11641 tgatgaccgt aatgaaaaaa tgggttataa aattagagaa gctcaaatgc aaaaaatacc
    11701 ttatcaaatc gtagttgggg ataaggaagt tgaaaataat caagtgaatg tgcgtcaata
    11761 tggatcgcaa gaccaagaaa cagttgaaaa agatgaattt atctggaatc tagttgatga
    11821 aattcgttta aaaaaacata gatagacagt tgtcgcaata aaatgctttg aaatttttat
    11881 tgcgtatcaa gtttcacagg gttgattatg cgtgatgaat cctgtatatt acaagttagt
    11941 taaaatatta aattgagtta gaggttgcat gtttaattag taacttgtca gaagtattta
    12001 tggtacataa gttgaacaag tgaaaggtaa agatgccgaa atagatataa accataaatt
    12061 atatctattg ggacagtttt cgaataggaa ctgtactgtc acagaatgtg atgtgctacc
    12121 ttatatagat aattgccaaa gtggttgcat atcttaaagg tatgtagcca cttttttact
    12181 tttaatatca ctatgttctg taaaaaaggg tatgaaagtg aataaaggtt atttatttct
    12241 tggcctctaa aacatggaaa gggagcttat atgtcaaaag ttcaaaatga aagtaacaat
    12301 gttgtcaaaa ggggacttaa agatcgtcat atttctatga ttgcgattgg gggttgtatt
    12361 ggtacaggtt tatttgtaac ttctggtgga gcaattcatg atgcaggtgc tttgggtgca
    12421 ttaataggat acgcaattat cggaataatg gtatttttct taatgacgtc acttggcgaa
    12481 atggctacgt atttgccagt atcaggttca tttagtacat atgctacaag atttgttgat
    12541 ccatctttag ggtttgcgct tggttggaac tattggttta actgggtagt aactgtagca
    12601 gcagatatta cgattgcagc acaagtcatt caatattgga caccattgca aggcataccc
    12661 gcttgggcat ggagtgcgtt gttcttagtt ataattttta gtctgaattc gttatcagtt
    12721 cgcgtctatg gtgaaagtga atactggttg gcattgataa aagtggttac agttattgtt
    12781 ttcattgcaa ttggtttatt aacgattgtc ggaatcatgg gtggtcatgt tgtaggattc
    12841 gaaatattta ataaaggtga aggtcctatt cttggtggca acttaggagg aagtttatta
    12901 tcaattctag gtgtattctt aatcgctggt ttctcattcc aaggtactga gttaattggt
    12961 attacggctg gtgaatcaga aaatcctgaa cgtgctgtgc cgaaagcaat taaacaagta
    13021 ttctggagaa ttttattatt ttacatttta gccatttttg ttatcggtat gttaattcct
    13081 tatgatagta gtgcattaat gggtggtagt gataatgtag caacgtctcc attcacatta
    13141 gtgtttaaaa atgctggatt tgcgtttgca gcatcattta tgaatgcagt cattttaacg
    13201 tctgtgttat cagcaggtaa ctcaggtatg tatgcttcaa ctcgaatgct atattcaatg
    13261 agtaaagata aattagcgtt tgaagcattt ggtaaaacaa ataaaaacgg tgtgccatat
    13321 atgtcgttac tcgttacagc aatcattgta gtgataattt tcgtattaca aagccttaca
    13381 aaaggtgcat acgaatatat cgttgcagca agtggtttaa ctggttttat tgcttgggta
    13441 ggtatagcag taagtcatta tcgatttaga agagcatttg ataaacaaaa ttatgacaaa
    13501 tctaaattaa aatatactgc taaattattc ccattcggac cattatttgc tggcttttta
    13561 tgtattgtag taattattgg tcaagatgtt gactttatta aaacaggaga ttttaatttt
    13621 agtcgcttct taattacgta tatgggaatc ccagtatttt tagcattctt tatttatcat
    13681 aagcttcgat ataaaacgaa aaaaattcca ttagaacaag tagatttacg tcaagacgtg
    13741 tcaatggatg aaataaaagg ttaaaataaa aataattaca gttatagtgt tgacttaaat
    13801 gtgaattgtt gatatgatta acaacgttga atacgaaacg aaccaagtag aagcacccgc
    13861 ttctcacctg tagcgacgca taaagcagtt ggcaggttaa tcacattaca cataactgtg
    13921 tataaggaag agcgggtatt ttatacctgc tcttcctttt tgcttggtgc aaatttcgta
    13981 aaaattttgg aggtgtcaac catagcaaaa gatcaaactc aaatcaatga caaaattcgt
    14041 gcaaaagaat tacgtttaat cggtcaagat ggtgaacaaa ttggtgttaa atcaaagcgt
    14101 gaagctttag aaatggctga acgtgtagat ttagacttag tggtcgttgc accgaatgcg
    14161 aaaccaccag ttgcaagaat tatggattac ggtaaattca aattcgaaca acagaaaaaa
    14221 gaaaaagaaa tgaaaaagaa acaaaaaatt atcaatgtta aagaaattcg tttaagtcca
    14281 acaattgagg aacatgattt ccaaactaag ttgaaaaacg gacgtaaatt cttaactaaa
    14341 ggcgataaat gtaaagtatc tattcgtttc agagggcgtg ccattacgca taaggaaatt
    14401 ggtcaacgtg tgctagaaaa atatgcagat gaatgcaaag atatagcaac agttgaacaa
    14461 aaacctaaaa tggacgggcg tcaaatgttt atcatgttag cgccaacagc tgaaaaataa
    14521 ttaaagctat tacaggagga cttatattat gccaaaaatg aaaactcacc gcggagcagc
    14581 taaacgtgtt aaaagaactg cttcaggtca attaaaacgt tcaagagctt tcacatctca
    14641 cttattcgca aacaagagca ctaaacaaaa acgtcaatta cgtaaagcta gattagtgtc
    14701 taagagcgat atgaaacgtg taaaacaatt attagcatac aaaaaataag aacaaataca
    14761 gaaaacggta ggaattacct aaggaggaat tttttatgcc acgagttaaa ggtggaacag
    14821 taacaagagc gcgtcgtaaa aaaacgatta aattagctaa aggttacttc ggttcaaaac
    14881 atacattata caaagtagct aagcaacaag taatgaaatc aggtcaatat gctttccgtg
    14941 accgtcgtca acgtaaacgt gacttccgta aattatggat tacacgtatc aacgcagcag
    15001 ctcgtcaaca tgaaatgagc tactcacgtt taatgaacgg tttgaaaaaa gctggtatcg
    15061 acattaaccg taaaatgtta tcagaaatcg caatttctga cgaaaaagca tttgctcaat
    15121 tagtaactaa agctaaagat gctttaaaat aataacactt ataaaaaaga gatggcattg
    15181 tcatctcttt tttatttttt aaaaagcaaa atgccacatt taatcgctaa aatatgacat
    15241 atttgctaaa atgcatttaa gagaacaaac attaaagtga ggagttttat acgatgtcta
    15301 aatttgatga acaaatcatt gtagtaccta gagaaattat ttttaataat gaaaagaata
    15361 atttcaatgg gtttttaaat aagaataaac cagaaggtca aaatattttt gatgcgctaa
    15421 gtcaatatga agttaagcga cgcggcgata tggaagagga tccatcatat aaacaactca
    15481 tttcttattg tttacttgaa aatgagcatg gcgagatatt agtgtatgaa cgattatctg
    15541 gcggtggaga agctcgattg catggacaat cttcaatagg tgtaggcggt catatgaatg
    15601 atgttccagg agcagaatct attaacgaag tattgagagt taatgcacag agagaattag
    15661 aagaagaagt aggtttaagt gagcaagatt cacaaaatat ggaatatatc ggttttatta
    15721 atgacgataa taatgaagtg ggcaaggtac atattggtgt tgtatttaaa atcactgtaa
    15781 gtacgaatga tgtagaagct aaagaaacag atactttacg aataaaatgg gttgaaaaag
    15841 gcaacataga gtcatatgat gatttcgaaa cgtggagcgc attaatcctt caagatttat
    15901 aatcaaacga ggtgacatat atgtcagata ttattcagtt tcctaactct tcgaaaaagt
    15961 tatataaaga tataaaacgt gctgaacaag accaaaacta tgacctgatg tatgaatata
    16021 ttgtgcaata tgaacgtcaa tttgaactca ctgaagaaat agcaatgatg aaatgccgta
    16081 tgttatatga gacggggtca tttcttgaat taagagaaga aacaattgtc ttattgaaaa
    16141 ctggcataca acaatatgat gcattgatga tttattacgt gaaaagtttg attggtttgg
    16201 gacaatattt tgaagcggta gaagtaattc atcaaattat tgatgaagtc aaagatcaca
    16261 aaacaagaat ggcattacat cctcttaaag aatttgctaa gtcaaaatta attgaagatg
    16321 aaaaacgatt gactcagtca ttaactgatt ttgatacgtt atcaatgagg gaacagacgc
    16381 acttgatttt gaagttaata gacaatggtc attttcaatt tcaagaaacg gtattatata
    16441 tattaaaatc taatacgtac agttataacc tcattagttt aatgattgag tatttaaggt
    16501 ttgcaaattg tacacaagaa ctgacaattg aaaagtatgg tatggatgta acttttgtac
    16561 cagctaattt aaatgggcta gaacatacaa cactgaaaga aaaagttata cctaacgtta
    16621 tggaaacatt aaatgatggt gcattacata tcgctgaaga agcacaccat attatgaata
    16681 atcactctat catgatttat ccaattgata ttgaaacttt gtttgaaacg aataaatgga
    16741 ttaatgcata cgaatgttat ttcaaaaata tgttaggact taaatgtgaa ttacaaagta
    16801 tcgatgcatt taattttatt cagcaattag atttgaataa taatagttaa attgttgcaa
    16861 aataatactc agcattttaa tatagttttg acgaaaaaag ttgatttaca aatatataaa
    16921 cgttgtgatt tcaatgtttg tatagggaat agacctatga tataataggg aagttgaaaa
    16981 aataaattag ttaatcatgg aggtatatat acatgacagc aacttgggaa aaaaaggaag
    17041 gtaacgaagg tttattaact gttactgttc ctgcagaaaa agtaaacaaa gcgttagatc
    17101 aagcattcaa aaaagtggtt aaacaaatta acgtacctgg attccgtaaa ggtaaagtgc
    17161 cacgtccaat ttttgaacaa cgctttggtg tagaagcatt atatcaagat gctatcgaca
    17221 ttttattacc agatgcttat ggtgaagcaa ttgacgaaac tgatattaaa ccagttgcac
    17281 aaccagaagt aagtgttact caaattgaaa aaggtaaaga tttcattttt gaagcaacag
    17341 ttacagttga gccagaagtt aaattaggag actataaagg tcttgaaatt gaaaaacaag
    17401 aaactgaatt atctgatgat gagttacaag aagcgattga ccacagctta ggacatttag
    17461 ctgaaatggt agttaaagaa gatggtgttg ttgaaaatgg cgacacagtt aacattgact
    17521 ttagtggttc agttgacgga gaagaattcg aaggtggaca agctgaaggt tacgatttag
    17581 aaatcggttc aggttcattc atacctggtt tcgaagagca attagaaggt atgaaagttg
    17641 acgaagaaaa agatgttgtc gtaacattcc cagaagaata ccatgctgaa gaattagctg
    17701 gtaaagaagc aactttcaaa acaaaagtta acgaaattaa atttaaagaa gttccagaat
    17761 taacagatga aattgctaat gaattagatg cagaagcaaa tacagtagac gagtacaaag
    17821 aaaacttacg taaacgttta gctgaacaaa aagctacaga tgctgaaaat gttgaaaaag
    17881 aagaagcgat tacaaaagct actgataata caacaatcga tattcctgaa gcaatggtta
    17941 atactgaatt agatcgtatg gtgtctgaat ttgcacaaag aattcaacaa caaggtttag
    18001 atttacaaac gtacttccaa atctcaggtc aagatgaaac tcaattaaga gagcaaatga
    18061 aagacgatgc agaacaacgt gttaaaacta acttaacttt aactgcgatc gctgaagctg
    18121 aaaaaatcga agctactgat gaagatatcg ataaagaatt agaaaaaatg agtaaacaat
    18181 ttaatatctc agttgaagat atcaaaaata ctttaggtaa tactgatatc attaaaaatg
    18241 atgttcgtat ccaaaaagtt atcgatttat taagagataa cgcaaagttc gttgaaggaa
    18301 ctaaagaaga ttaatcctca ttaaatatta aattacaaaa atgagtagca gatgcatagc
    18361 ttatgtatct gctactattc tttaagcaaa aagtttgtat gttaatatgt tgcattgtaa
    18421 tatccaatct agtatagtct ttaacgaata ggggtgtaaa aagaatgttt aaattcaatg
    18481 aagatgaaga aaatttgaaa tgctctttct gcggaaaaga ccaagatcaa gtaaaaaaac
    18541 ttgtagcagg aagtggtgta tatatttgta atgagtgtat tgaattatgc tcagaaatcg
    18601 tcgaagaaga attagctcaa aacacttctg aagcgatgac agaattacct actcctaaag
    18661 aaattatgga tcatttaaac gaatatgtta ttggtcaaga aaaagctaaa aaatctttag
    18721 ctgtagctgt ttataaccac tataagcgta ttcaacaatt aggaccaaaa gaagatgatg
    18781 ttgaattaca aaaaagtaac attgcattaa ttgggccaac aggtagtggt aaaacattat
    18841 tagctcaaac attagccaag acgttgaatg taccatttgc aattgcagat gcgacaagtt
    18901 taactgaagc tggttatgta ggcgatgatg ttgaaaatat cttgttgaga ttaattcaag
    18961 cagctgactt tgacattgat aaagccgaaa aaggtattat ttatgtagat gaaattgata
    19021 aaattgcacg taaatctgaa aacacatcta taacacgtga cgtttcaggt gaaggtgttc
    19081 aacaagcatt gcttaaaatc ttagaaggta cgactgcaag tgttccgcca caaggtggac
    19141 gcaaacatcc aaaccaagaa atgattcaaa ttgatacaac aaatatctta tttattcttg
    19201 gtggtgcctt tgatggtatt gaagaagtga ttaagcgccg tcttggtgaa aaagttattg
    19261 gtttctcaag caatgaagct gataaatatg acgaacaagc attattagca caaattcgcc
    19321 cagaagattt gcaagcctat ggtttgattc ctgaatttat cggacgtgtg ccaattgtag
    19381 ctaatttaga aacattagat gtaactgcgt tgaaaaacat cttaacgcaa cctaaaaatg
    19441 cacttgtgaa acaatatact aaaatgctgg aattagataa tgtggattta gagttcactg
    19501 aagaagcttt atcagcaatt agtgaaaaag caattgaaag aaaaacaggt gcgcgtggtt
    19561 tacgttcaat catagaagaa tcgttaatcg atattatgtt tgatgtgcct tctaacgaaa
    19621 atgtaacgaa ggtagttatt acagcacaaa caattaatga agaaactgaa ccagaactat
    19681 acgacgcaga aggcaattta attaataata gtaaaacatc agcttaatca ttgatgtgtt
    19741 ataaaagtga aaagctccaa tcaaagttga cgctcaaata gtttaacttt gatcggagct
    19801 tttttataat gtataccgaa aggttcaaat attatataat ggt
//
LOCUS       DAMZKC010000036        19816 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.036, whole genome shotgun sequence.
ACCESSION   DAMZKC010000036 DAMZKC010000000
VERSION     DAMZKC010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 19816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19816
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.036"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            140..580
                     /locus_tag="POB55_000954"
     CDS             140..580
                     /locus_tag="POB55_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DM13 domain-containing protein"
                     /protein_id="HDI6499928.1"
                     /translation="MNTKYFLAVGAVASVLTLGACGNSNSQDQGNKTEQKTKSEDSNV
                     KTDKTKHLTGTFSSKNGETVEGKAEIKNGKLMLTNYKSSKGPDLYVYLTKNGDIKNGK
                     EIAMVDYDKEKQTFDLKNVDLSKYDEVTIYCKKAHVIFGGAKLK"
     gene            923..1396
                     /locus_tag="POB55_000955"
     CDS             923..1396
                     /locus_tag="POB55_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDI6499929.1"
                     /translation="MNKLLLLVTFIIRVGSGIVMLMQGYEKLTGGFTLKGLVPVIANN
                     TDSPEWYKWFFANIVAHTTSLFDIVVPLGEIAIGLGLIFGVFAYAASFFGAFVMINYI
                     LADMIFTYPLQLTFFILLLMSHSLLKQISLKEIINYFRGRKNRGEKIDDPLTDRG"
     gene            1371..2057
                     /locus_tag="POB55_000956"
     CDS             1371..2057
                     /locus_tag="POB55_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDI6499930.1"
                     /translation="MTHLLIVDDEQDIVDICQTYFEYEGYKVTTTTSGKEAISLLSND
                     IDIMVLDIMMPEVNGYDIVKEMKRQKLDIPFIYLTAKTQEHDTIYALTLGADDYVKKP
                     FSPRELVLRINNLLTRMKKYHHQPVEQLSFDELTLINLSKVVTVNGHEVPMRIKEFEL
                     LWYLASRENEVISKSELLEKVWGYDYYEDANTVNVHIHRIREKLEKESFTTYTITTVW
                     GLGYKFERSR"
     gene            2057..3112
                     /gene="saeS"
                     /locus_tag="POB55_000957"
     CDS             2057..3112
                     /gene="saeS"
                     /locus_tag="POB55_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     SaeS"
                     /protein_id="HDI6499931.1"
                     /translation="MVLSIRSQIIIGVVSSILLTSTILAIAYILMWFNGHMTLTLTLT
                     TIITSCLTLLICSIFINPLIQKIKQFNIKTKQFANGNYASNDKTFNSPKEIYELNQSF
                     NKMASEITQQMNQIKSEQQEKTELIQNLAHDLKTPLASIISYSEGLRDGIITKDHEIK
                     ESYDILIKQANRLSTLFDDMTHIITLNTGKTYPPELIQLDQLLVSILQPYEQRIKHEN
                     RTLEVNFCSEIDAFYQYRTPLERILTNLLDNALKFSNVGSRIDINISENKDQDTIDIA
                     ISDEGIGIIPELQERIFERTFRVENSRNTKTGGSGLGLYIANELAQQNNAKISVSSDI
                     DVGTTMTVTLHKLDITS"
     gene            complement(3184..4167)
                     /gene="csbB"
                     /locus_tag="POB55_000958"
     CDS             complement(3184..4167)
                     /gene="csbB"
                     /locus_tag="POB55_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoteichoic acid-specific glycosylation protein
                     CsbB"
                     /protein_id="HDI6499932.1"
                     /translation="MKIRVVIPCFNEGEVITQTHQQLTEILSQDSSVKGYDYNMLFID
                     DGSTDTTIDEMQHLATIDRHVSFISFSRNFGKEAAMIAGYQHSTEFDAVIMIDCDLQH
                     PPEYIPKMVEGFMEGYDQVIAKRDRSGENFSRKTLSHLYYKLVNCFVEEVQFDDGVGD
                     FRLLSQRAVKSIASLEEYNRFSKGLFEWIGYNTKVFTYQNVERQKGESKWSFKKLFNY
                     GIDGLISFNSKPLRMMIYLGLFIFSISVLYIIYLFINIMISGVNIPGYFSTIAAILLL
                     GGIQLISIGVVGEYIGRIYYEVKARPKYIIQATNLSSIENDEKDTHKVYSK"
     gene            complement(4299..5138)
                     /locus_tag="POB55_000959"
     CDS             complement(4299..5138)
                     /locus_tag="POB55_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDI6499933.1"
                     /translation="MLNEIQILNNGYPMPSVGLGVYKISDEDMTKVVNAAIDAGYRAF
                     DTAYFYDNEASLGRALKDNGVDREDLFITTKLWNDYQGYEKTFEYFNKSIENLQTDYL
                     DLFLIHWPCEADGLFLETYKAMEELYEQGKVKAIGVCNFNVHHLEKLMAQSSIKPMVN
                     QIEVHPYFNQQELQEFCDRHDIKVTAWMPLMRNRGLLDNPVIVKIAEKYHKTPAQVVL
                     RWHLAHNRIIIPKSQTPKRIQENIDILDFNLELTEVAEIDALNRNARQGKNPDDVKIG
                     DLK"
     gene            complement(5360..6709)
                     /locus_tag="POB55_000960"
     CDS             complement(5360..6709)
                     /locus_tag="POB55_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDI6499934.1"
                     /translation="METSTIISLIIFILLIALTTVFVGSEFALVKIRATRIEQLADEG
                     NKPAKIVKKMIANLDYYLSACQLGITVTSLGLGWLGEPTFEKLLHPIFEAINLPTALT
                     TTISFAVSFIIVTYLHVVLGELAPKSIAIQHTEKLALVYARPLFYFGNIMKPLIWLMN
                     GSARVIIRMFGVNPDAQTDAMSEEEIKIIINNSYNGGEINQTELAYMQNIFSFDERHA
                     KDIMVPRTQMITLNEPFNVDELLETIKEHQFTRYPITDDGDKDHIKGFINVKEFLTEY
                     ASGKTIKIANYIHELPMISETTRISDALIRMQREHVHMSLIIDEYGGTAGILTMEDIL
                     EEIVGEIRDEFDDDEVNDIVKIDNKTFQVNGRVLLDDLTEEFGIEFDDSEDIDTIGGW
                     LQSRNTNLQKDDYVDTTYDRWVVSEIDNHQIIWVILNYEFNEARPTIGQSDEDEKSE"
     gene            complement(6930..8111)
                     /gene="nagA"
                     /locus_tag="POB55_000961"
     CDS             complement(6930..8111)
                     /gene="nagA"
                     /locus_tag="POB55_000961"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDI6499935.1"
                     /translation="MSELIIYNGKVYTEDGKIDNGYIHVKDGQIVAIGEGDDKAAIDN
                     DTTNKIQVIDAKGHHVLPGFIDIHIHGGYGQDAMDGSYDGLKYLSENLLSEGTTSYLA
                     TTMTQSTDKIDKALTNIAKYEAEQDVHNAAEIVGIHLEGPFISENKVGAQHPQYVVRP
                     FIDKIKHFQETANRLIKIMTFAPEVEGAKEALETYKDDIIFSIGHTVATYEEAVEAVE
                     RGAKHVTHLYNAATPFQHREPGVFGAAWLNDALHTEMIVDGTHSHPASVAIAYRMKGN
                     ERFYLITDAMRAKGMPEGEYDLGGQKVTVQSQQARLANGALAGSILKMNHGLRNLISF
                     TGDTLDHLWRVTSLNQAIALGIDDRKGSIKVNKDADLVILDDDMNVKSTIKQGKVHTF
                     S"
     gene            complement(8419..10377)
                     /locus_tag="POB55_000962"
     CDS             complement(8419..10377)
                     /locus_tag="POB55_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDI6499936.1"
                     /translation="MRVTELLTKDTIAMDLMANDKNGVIDELVNQLDKAGKLSDVASF
                     KEAIHNRESQSTTGIGEGIAIPHAKVAAVKSPAIAFGKSKAGVDYQSLDMQPAHLFFM
                     IAAPEGGAQTHLDALAKLSGILMDENVREKLLHASSPEEVLAIIDEADDEVTKEEEAE
                     AEAQQVATAEQSSKQSNEPYVLAVTACPTGIAHTYMASDALKKQADKMGIKIKVETNG
                     SSGIKNHLTEQDIENATGIIVAADVHVETDRFDGKNVVEVPVADGIKRPEELINKALD
                     TSRKPFVARDGQRKGNSNDSQEKLSPGKAFYKHLMNGVSNMLPLVISGGILMAIVFLF
                     GVDSFKPKSSEYNAFAEQLWNIGSKSAFALIIPILSGFIARSIADKPGFAAGLVGGML
                     AISGGSGFIGGIIAGFLAGYLTQGVKAMTRKLPQALEGLKPTLIYPLLTVTATGLLMI
                     YVFNPPASWLNHLLLDGLNNLSGSNIVLLGLVIGAMMAIDMGGPFNKAAYVFATGALI
                     EGNAAPITAAMIGGMIPPLAIATAMLIFRRKFTKEQRGSIIPNYVMGMSFITEGAIPF
                     AAADPLRVIPSMMIGSGIGGAIALGLGSRITAPHGGIIVIVGTDGAHLLQTLIALLVG
                     TLVSALIYGLIKPKLTETEIEASKSMDE"
     gene            complement(10383..11303)
                     /gene="pfkB"
                     /locus_tag="POB55_000963"
     CDS             complement(10383..11303)
                     /gene="pfkB"
                     /locus_tag="POB55_000963"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDI6499937.1"
                     /translation="MIYTVTFNPSIDYVIFTNDFKIDGLNRATATYKFAGGKGINVSR
                     VLKTLDVESTALGFAGGFPGKFIIDTLNNSAIQSNFIEVDEDTRINVKLKTGQETEIN
                     APGPHITSTQFEQLLQQIKNTTSEDIVIVAGSVPSSIPSDAYAQIAQITAQTGAKLVV
                     DAEKELAESVLPYHPLFIKPNKDELEVMFNTTVNSDTDVIKYGRLLVDKGAQSVIVSL
                     GGDGAIYIDKEISIKAVNPQGKVVNTVGSGDSTVAGMVAGIASGLTIEKAFQQAVACG
                     TATAFDEDLATRDAIEKIKSQVTISVLDGE"
     gene            complement(11300..12064)
                     /locus_tag="POB55_000964"
     CDS             complement(11300..12064)
                     /locus_tag="POB55_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDI6499938.1"
                     /translation="MMIITEKRHELILEELSHKDFLTLQELIDRTGCSASTIRRDLSK
                     LQQLGKLQRVHGGAMLKENRMVEANLTEKLATNLDEKKMIAKIAANQINDNECLFIDA
                     GSSTLELIKYIQAKDIIVVTNGLTHVEALLKKGIKTIMLGGQVKENTLATIGSSAMEI
                     LRRYCFDKAFIGMNGLDIELGLTTPDEQEALVKQTAMSLANQSFVLIDHSKFNKVYFA
                     RVPLLESTTIITSEKALNQESLKEYQQKYHFIGGTL"
     gene            complement(12314..12796)
                     /gene="ybaK"
                     /locus_tag="POB55_000965"
     CDS             complement(12314..12796)
                     /gene="ybaK"
                     /locus_tag="POB55_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HDI6499939.1"
                     /translation="MAKNKKTNAMRMLDRAKIKYEVHSFEVPEEHLSGQEVAELIQAN
                     VKTVFKTLVLENTKHEHFVFVIPVSETLDMKKAAALVGEKKLQLMPLDNLKNVTGYIR
                     GGCSPVGMKTLFPTVVDKSCENYSHISVSGGLRTMQITIAVEDLITITKGKIGAVIHE
                     "
     gene            complement(12969..13421)
                     /locus_tag="POB55_000966"
     CDS             complement(12969..13421)
                     /locus_tag="POB55_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499940.1"
                     /translation="MTVKNLFLGFVAVILTVCLIGLLILATNEDALAKVHKTINTLNA
                     INVSTEDTYKKKMDILNIHTAKASEVNENMKKQNHFKHRVNANKSNSFNEQECQVIAD
                     RYADKHINDNYGLERISKTNHGYNYVYSNDNSTSKQHVSISNQGIITK"
     gene            complement(13714..14880)
                     /gene="norA"
                     /locus_tag="POB55_000967"
     CDS             complement(13714..14880)
                     /gene="norA"
                     /locus_tag="POB55_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter NorA"
                     /protein_id="HDI6499941.1"
                     /translation="MNKQIFVLYFNIFLIFLGIGLVIPVLPVYLKDLGLTGSDLGLLV
                     AAFALSQMIISPFGGTLADKLGKKLIICIGLILFSVSEFMFAVGHNFSVLMLSRVIGG
                     MSAGMVMPGVTGLIADISPSHQKAKNFGYMSAIINSGFILGPGIGGFMAEVSHRMPFY
                     FAGALGILAFIMSIVLIHDPKKSTTSGFQKLEPQLLTKINWKVFITPVILTLVLSFGL
                     SAFETLYSLYTADKVNYSPKDISIAITGGGIFGALFQIYFFDKFMKYFSELTFIAWSL
                     LYSVVVLILLVFANGYWSIMLISFVVFIGFDMIRPAITNYFSNIAGERQGFAGGLNST
                     FTSMGNFIGPLIAGALFDVHIEAPIYMAIGVSLAGVVIVLIEKQHRAKLKEQNM"
     gene            15089..15511
                     /locus_tag="POB55_000968"
     CDS             15089..15511
                     /locus_tag="POB55_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDI6499942.1"
                     /translation="MKLQKSNHTILLVLEKGEDIVECITTFADDQDLTFTSVSGIGAC
                     DDVVLKFFNLTTKQYEEKHITEPLELTSLLGNISRLDNGHFAHLHATFGTQSYETFSG
                     HLAKAIVSATAEIILTVTDLDIQRSFKDAVGLNLLDPQ"
     gene            15697..16314
                     /locus_tag="POB55_000969"
     CDS             15697..16314
                     /locus_tag="POB55_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HDI6499943.1"
                     /translation="MQSRYIARIYFLILFIVSLFETKIFQFMTLNLFLAYIPFELSLL
                     LRLFKPNKKYEWPLFIIYSFIFVLLLPNTFYMVTDLIHLNQFQFNFYAGLNLSEWKYF
                     TYLLLGVFLAVYVMILIYFEILTLTSHRWLNRLIVVVLMFLNGFGIYIGRFLRLHSVY
                     FFNEPLHVLNHILESLTLKTAMFVCFMVVMQAAILLFGKGVRLNK"
     gene            16311..16595
                     /locus_tag="POB55_000970"
     CDS             16311..16595
                     /locus_tag="POB55_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499944.1"
                     /translation="MTINIIVLIVSIIVFQLIIGHIWHDIGLSYLRSILLMMLPFGLG
                     VFIQQVSYYERQYPKWQVPQNIKVRLKYIYLATFLEYVVLYLTLFTDILR"
     gene            complement(16750..18123)
                     /locus_tag="POB55_000971"
     CDS             complement(16750..18123)
                     /locus_tag="POB55_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HDI6499945.1"
                     /translation="MAIAVLLNRMFRMEHNPLFEYIYQQKEDIDACYFIIPEEDMSSA
                     SDLKAQFYRGTLQRFYQSLHAEKLTPYVMSYDDIISFCKENNISEVVTAGDIMSYHLE
                     EYDILHQRSLFNEARIAVTLIRGNHYFKASKTMNQQGEPYKVFTSFYKKWRPYLRHRD
                     VYHYDLKSFEDFVIASPDDLVFDDIAFGSSQIIEQNKWQHFLDQDIQNYESGRDYLPE
                     VLTSQLSVALAYGLLDIIEIFNDLLARYDEDEANYEAFIRELIFREFYYVLMTQYPET
                     SYQAFKPKYRQIKWSQNEADFNAWCEGQTGFPIIDAAIMELTQTGFMHNRMRMVVSQF
                     LTKDLFIDWTWGEKFFRKHLIDYDAASNIHGWQWSASTGTDAVPYFRMFNPIRQSERF
                     DAKALYIKTYLPIFNQIDAKYLHDTQRNESNLFEQGIELGSHYPRQMVDHQEKRTQVL
                     ATFKALD"
     gene            complement(18209..19762)
                     /locus_tag="POB55_000972"
     CDS             complement(18209..19762)
                     /locus_tag="POB55_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HDI6499946.1"
                     /translation="MSEEKHVVEHEQQKKEKTKKQYKPFWIVMSFIILIVVLLLPAPS
                     SLPIMAKAVLAILAFAVIMWVTEAVSYPVTATLIIGLMILLLGFSPVQNLGEKLGNPK
                     SGSAILAGSDLLGTNHALSLAFSGYATSAVALVAAALFLAAAMQETNLHKRLALLVLS
                     IVGNKTRNIVIGAIIVSIVLAFFVPSATARAGAVVPILLGMIAAFKVSKDSKLASLLI
                     ITSVQAVSIWNIGIKTAAAQNIVAINFINHQLGFDVSWGEWFLYAAPWSIVMSVALYF
                     IMIKVMPPEINTIEGGKDLIKEELHKLGPVSPREWRLIVISMLLLLFWSTEKVLHPID
                     SASITIIALGVMLMPKIGVMTWKHVDNKIPWGTIIVFGVGISLGNVLLKTGAAQWLSD
                     QTFGVLGLKHLPIIATIALITLFNILIHLGFASATSLSSALIPVFISLTSTLHLGDQS
                     IGFVLIQQFVISFGFLLPVSAPQNMLAYGTGTFTVKDFLKAGIPLTIVGYILVIVFSM
                     TYWKWLGLL"
BASE COUNT         6547 a         3660 c         2780 g         6829 t
ORIGIN      
        1 caattatttt cattttcaaa ttattctttc ttcaatatta gttaagcgat atttaaacga
       61 agttaagaat tagttaatgg catattattt gccttcattt taaacttaac ttatcaaatt
      121 gaagaaatga ggagttagca tgaatacaaa atatttttta gcagttggtg ctgttgcctc
      181 cgtattaact ttaggcgctt gtggtaattc taattcacaa gatcaaggta acaaaactga
      241 acaaaaaaca aagtcagaag atagcaatgt taaaactgat aaaacaaaac acctaacagg
      301 tacattcagt tctaaaaacg gtgaaactgt tgaaggtaaa gctgagatta aaaatggtaa
      361 attaatgctt actaactaca aatcatcaaa aggtccagat ttatacgtct acctaacaaa
      421 aaatggcgac attaaaaacg gtaaagaaat cgcaatggtt gactacgata aagaaaaaca
      481 aacatttgat cttaaaaatg tagatttaag caaatatgat gaagtaacaa tctattgtaa
      541 aaaagctcac gtcatcttcg gcggcgctaa attaaaataa tcaatttctg agttaaactt
      601 ttatttacaa catactatta ctatacatta caaattttaa aaatatacat tacactcatt
      661 actcaatgga aagcgtatga tttcacagcc cccctagctt gtagaaatca tactttcctt
      721 ttttcaatat atatacaact attaaatccc ataagattgt agagcacata agtaaaattt
      781 ttttagagct tgaggtttgt ttagcttaag caacccatga gctcaaacac ttcctgttca
      841 cataacacta caaatcgcat tatgttgctt aatcttatgt ttatataaat tacacacaat
      901 aaatagaaag aatgtgaaca tcatgaataa attattgcta ctcgttacat ttatcattcg
      961 tgtgggttca ggtattgtta tgttaatgca aggctacgaa aaattaacgg gcggatttac
     1021 gctgaaaggt ttagtaccag tcatcgctaa caatactgat tcaccagagt ggtataagtg
     1081 gtttttcgca aatatagttg cacatacgac gtcattattt gatattgttg tcccactcgg
     1141 agagattgca attggattag gtttaatttt tggagttttt gcatatgctg ctagtttctt
     1201 tggagccttt gttatgataa attatatctt agcagatatg atatttacgt atcctcttca
     1261 attaactttc tttatccttt tactaatgag tcactcattg ttaaaacaga tttcacttaa
     1321 agaaatcatt aattacttta gaggtcgtaa gaacagaggt gaaaaaatag atgacccact
     1381 tactgatcgt ggatgatgaa caagacattg tagacatttg tcaaacctat tttgaatatg
     1441 aaggttacaa agtaacaacg acaactagcg gtaaagaagc aatttcttta ctatcaaatg
     1501 atattgatat catggtactt gatatcatga tgccagaagt taatggttac gacattgtca
     1561 aagaaatgaa aaggcaaaaa ttagatatcc cctttatcta tttaactgcc aaaacacaag
     1621 aacatgatac catttacgcc ttaactttag gtgcagatga ctatgtcaaa aaaccattta
     1681 gtccaaggga actcgtttta cgtattaata atttacttac aagaatgaag aaataccatc
     1741 atcaaccagt tgaacaactg tcgtttgatg aattaacact tattaactta agtaaagttg
     1801 tgactgtaaa tggtcacgaa gtccctatgc gtattaagga atttgagtta ttgtggtatt
     1861 tagcttctag agaaaatgaa gttatttcta aatcagaatt acttgaaaaa gtttggggat
     1921 atgactatta cgaagatgct aataccgtga atgtccatat acaccgtatt agagaaaaat
     1981 tagaaaaaga gagctttaca acatatacca tcacaactgt atggggatta ggatataaat
     2041 ttgaaaggag ccgataatgg tgttatcaat tagaagtcaa atcattattg gcgtcgtatc
     2101 gagtatacta ttaacttcaa ctattttagc aattgcatat attttaatgt ggtttaacgg
     2161 ccatatgaca ctaactttga ccttaacgac aataattaca agctgtttaa ccttattaat
     2221 atgtagtatt tttattaatc cacttataca aaaaattaag cagtttaata taaaaactaa
     2281 gcaatttgct aacggaaatt acgcaagcaa tgataaaacg tttaattcac caaaagaaat
     2341 ttatgaatta aatcaatctt ttaataaaat ggcttctgaa attacgcaac aaatgaatca
     2401 aattaaatcc gaacaacaag aaaaaacaga actgattcaa aacttagccc atgatttaaa
     2461 aacaccttta gcaagcatta tttcatattc tgaaggacta cgtgatggta taatcacaaa
     2521 ggatcatgag attaaagagt catacgacat attaattaaa caagcaaaca gattatcaac
     2581 attatttgat gatatgactc atattatcac tttaaataca ggtaaaacat atcccccaga
     2641 attaatacaa ctagaccaat tacttgtatc aatattgcaa ccatatgagc aacgtatcaa
     2701 acatgaaaac cgcacattag aagtgaattt ctgtagcgaa attgatgcat tttatcaata
     2761 tcgaacgcca cttgagcgta ttttaacaaa cttacttgat aatgcgctaa aattttcaaa
     2821 tgttggtagt cgcattgata ttaatattag tgaaaacaaa gatcaagata ctatcgacat
     2881 tgctattagc gatgaaggta ttggcattat accagaacta caagaacgta tattcgaacg
     2941 tacattcaga gtagaaaact ctcgtaatac aaaaacgggt ggttctggat taggcttata
     3001 tatagctaat gaactcgcgc aacaaaataa cgcaaaaatc agtgtaagca gtgatataga
     3061 tgtaggaact acgatgactg taacattaca caaattagac attacgtcat aatccgattt
     3121 atttataaaa taaaatgcaa agactaaaaa gaagctccca ttaatgaggg cttctttttt
     3181 tgtttattta gaataaactt tatgggtatc cttctcatca ttttcaatac ttgaaagatt
     3241 tgtagcttga ataatatatt taggacgtgc cttaacttca taatatatcc tgccaatata
     3301 ttcacctaca acaccaattg aaattaactg tatgccgcct aataataaaa tagctgcaat
     3361 cgttgaaaaa tatcccggaa tattaacacc agatatcata atattgatga ataaatagat
     3421 aatatatagc acgcttattg aaaagataaa caagccaaga taaatcatca ttctcaaagg
     3481 tttactatta aaggaaatca atccatcaat accataatta aataactttt taaaggacca
     3541 cttagattcc cctttttgtc tctcaacatt ttgatacgta aacactttag tattatagcc
     3601 tatccattca aataaccctt ttgaaaatcg attatattct tcaagtgatg caatggattt
     3661 aacagctctt tggcttaaaa gtctaaaatc accaacacca tcatcaaatt gtacttcttc
     3721 tacaaagcaa ttaactaact tataatacaa atggcttaat gttttgcgac taaaattttc
     3781 accacttcta tcacgctttg caatcacttg atcatagcct tccataaaac cttcaaccat
     3841 tttcggaata tattcaggtg gatgttgcaa atcacaatct atcatgatga ctgcatcaaa
     3901 ttcagtacta tgctggtaac ctgcaatcat agctgcttct tttccaaaat ttctactaaa
     3961 agaaataaag ctgacatgcc tatctattgt ggcaagatgt tgcatttcat ctatagtggt
     4021 atccgtacta ccatcatcta tgaaaagcat attataatca tagcctttca cactactatc
     4081 ttgtgaaagt atttcagtta attgttgatg tgtttgtgta atgacttccc cttcattaaa
     4141 acaaggaatg acgactctaa ttttcatcta tgtccatcct ttttatttaa aagattttga
     4201 atgtacctga gtattgtttt atcaaatagt aaaaaccaac tcatttattt taatgtacac
     4261 attaaaaggc attcataaac gtaaaattta acatccagtt attttaaatc cccaattttc
     4321 acatcatctg gatttttacc ttgtcttgca tttctattta aagcatcaat ttcagctact
     4381 tctgttaatt ctaaattaaa atctaaaata tctatatttt cttgaatgcg tttaggtgtc
     4441 tgagattttg gaataataat tctattgtgt gctaaatgcc aacgtaatac aacttgtgct
     4501 ggtgttttat gatatttttc agcaatttta acaatgacag ggttgtctag tagtcctcta
     4561 tttctcatca aaggcatcca tgcagtcact ttaatatcgt gacgatcaca aaattcttgt
     4621 aattcttgtt ggttaaaata tggatgtacc tcaatttgat tcaccattgg tttgatactt
     4681 gattgagcca ttaatttttc tagatgatga acattaaaat tacatacacc tattgctttt
     4741 accttacctt gctcgtaaag ttcttccata gctttatatg tttctaaaaa tagaccatct
     4801 gcttcacaag gccaatgtat tagaaataaa tcaagataat cagtttgtaa attttcaatc
     4861 gatttgttga aatattcgaa tgttttctca taaccttgat agtcattcca taacttcgtt
     4921 gttataaaca aatcttctct atcgacgcca ttatccttta atgctcgtcc tagtgaagcc
     4981 tcattatcat aaaagtatgc tgtatcaaac gctctatagc ctgcgtcaat tgcagcattt
     5041 acaactttag tcatatcttc gtcagagatt ttataaacac ctaatccaac tgaaggcatc
     5101 gggtatccat tatttaatat ttgtatctca ttcaacatct ttatatctct ccaatctatg
     5161 tatcttttat atctttacat taccctaaat ttttcaataa actcaattaa tacgaattat
     5221 cgctttcaat aaaaaatatt cattaaatca ttaaagatat tgagttccaa tactattttc
     5281 acttttcatt aattttagtc aaaaaaataa ccaaccaaaa atgaattaaa tcattcttag
     5341 ttggtttata tattaatatc tattctgatt tttcatcttc atcagactgt ccgatagtag
     5401 gtctcgcttc attaaattca tagtttaata tcacccaaat aatttggtgg ttatcgattt
     5461 ctgaaacaac ccagcgatca taagttgtat ccacgtaatc atctttttgt aaattggtat
     5521 tacgagattg taaccatcca cctatcgtat caatatcctc agagtcatca aattctatac
     5581 cgaactcttc agttaaatca tccaatagta ctctgccatt tacttggaat gtcttattat
     5641 caattttaac gatatcattc acttcatcat catcaaattc atcacgaatt tctccaacga
     5701 tttcttctaa aatatcttcc atcgttaaaa tacctgccgt tccaccatat tcatctataa
     5761 taagactcat atgtacatgt tcacgttgca ttctaattaa tgcatcactg atacgtgttg
     5821 tctctgaaat cattggcaac tcatgtatat agtttgctat tttaatcgtt tttccagaag
     5881 cgtattcagt taaaaattct ttgacgttaa taaatccttt aatgtggtct ttatcaccat
     5941 catcagtaat tggataacgc gtaaattgat gttcttttat tgtttctagt aattcgtcta
     6001 cattaaaagg ttcatttagt gtaatcattt gagttctagg taccattata tcttttgcat
     6061 gtctttcatc gaatgaaaag atattttgca tatatgccaa ttcagtttgg ttgatttctc
     6121 caccattata actattgtta ataataattt tgatttcttc ttctgacatt gcatcagttt
     6181 gggcatcagg atttacacca aacattctaa taataacacg tgcagaacca ttcatcagcc
     6241 aaatcaatgg tttcataatg ttaccgaaat agaacaatgg tcttgcatat actaaagcaa
     6301 gcttttcagt atgttgaatc gctatagatt taggcgctaa ttcaccaagt actacatgca
     6361 aatacgtaac gattataaat gacactgcaa acgaaatcgt cgtcgttaat gcagttggta
     6421 aattgattgc ttcaaatatt gggtgtaata gcttttcaaa cgttggttca ccaagccaac
     6481 ctaaccctaa agatgttact gttataccta actgacaagc agaaagataa taatctagat
     6541 tagcaatcat cttttttact attttagcag gtttatttcc ttcatctgct agctgttcaa
     6601 ttcttgttgc tctaattttt actaatgcaa attctgaacc aacaaataca gtggttaatg
     6661 caattaatag aataaatata atcaaactaa ttatggtcga agtttccaat taattcccta
     6721 tttctaggga ttcacctcca tgttatatgt gtcactcatg ggtaacacgc attcaaattt
     6781 atcactatga cttaaattac aactacatgt tatcgccttc ccatttagac acccccagaa
     6841 aaaaatgtta ttgcttctat tttatcataa tataaagtgc ttatgttaac agattaaatc
     6901 tattgcatac atttaattat gattatttat tagctaaatg tgtgaacctt accttgtttt
     6961 attgtagatt ttacattcat atcatcatct agaataacaa gatctgcatc cttatttact
     7021 ttaatactac cttttctatc atcgatacct aatgcaatgg cttgatttaa acttgttact
     7081 cgccataaat gatctaatgt atcacctgta aatgatatta agttacgtaa cccatgattc
     7141 atttttaaaa tactaccagc aagcgcacca tttgcaagac gtgcttgttg cgattgaaca
     7201 gttacttttt gtccacccaa atcatattct ccttcaggca taccttttgc acgcattgca
     7261 tcggtaatta aataaaaacg ttcattacct ttcatacggt aagcaatagc aaccgatgcc
     7321 ggatgagaat gtgtgccatc aacaatcatt tcggtatgta gagcatcatt caaccatgct
     7381 gctccaaaaa cacctggttc tctatgctgg aatggcgtcg ctgcattata taaatgcgtg
     7441 acatgtttag ctcctcgctc aacagcttcg actgcttctt cgtatgttgc cactgtatga
     7501 ccaattgaaa aaataatgtc atctttatac gtttcaagcg cttcttttgc accttcaact
     7561 tcaggtgcaa acgtcataat ctttattaat ctgttagcag tctcttgaaa atgtttaatt
     7621 ttatcgataa atgggcgtac aacgtattgc ggatgttgag caccaacttt attttcagat
     7681 ataaatggtc cttctaaatg tatacctaca atttccgctg cattgtgaac atcttgctcc
     7741 gcttcatatt tagcaatatt tgtaagtgct ttatctattt tatcagtaga ttgcgtcatt
     7801 gtagtggcca agtatgatgt cgtcccttca gataacaaat tttcggatag atattttaag
     7861 ccatcgtatg acccatccat tgcatcttga ccataaccac catgaatatg tatatcaata
     7921 aaacctggta atacatgatg acctttagca tcaatcactt gaattttatt tgtcgtatca
     7981 ttatcaattg ctgctttatc atccccttct ccaattgcaa caatctgtcc atctttcaca
     8041 tgaatgtaac cattatcgat tttgccatct tcagtataaa ctttgccgtt atatataatt
     8101 aattctgaca cgtataaacc cttctttatt tataaatttt cactttaaga cactcatatt
     8161 ttactatatc tgcttcgttt ggcataactt tttgcaaaat attaaaaaag tctatacatt
     8221 tgtggtgtct atttcttcat tcatttatct aaaaataaaa acaccttcat atcaattcat
     8281 aaaaactgaa ttaatagaaa gtgtttttac agctttgtat aagctaccgt taacacgatg
     8341 tataaaacta acatatattc attgaactta caacttaata tgaagctctt tgctaacaat
     8401 cattttacat cattaaaact actcgtccat tgattttgaa gcttcgattt ctgtttcagt
     8461 taacttgggt ttgattaaac cgtaaattaa tgctgaaact aatgtaccaa ctagaagtgc
     8521 aataagagtt tgaagtaagt gtgcaccatc cgtaccaaca attacaataa taccaccatg
     8581 tggcgcagta attcgtgaac ctaagcctaa agcaattgcg ccacctatac ctgaaccaat
     8641 catcattgaa ggaataacac gtaatggatc ggcagctgca aatggaatcg caccttctgt
     8701 aataaaggac atacccatca catagttagg gataattgaa ccacgttgtt cttttgtaaa
     8761 tttacgtctg aaaattaaca tcgctgtcgc aattgctaac ggtggaatca taccaccaat
     8821 cattgcagct gtaattggtg ctgcattacc ttcaatcaac gcacctgttg caaaaacata
     8881 tgccgctttg ttgaatggac cgcccatatc aattgccatc atagcgccaa taactaaacc
     8941 taataataca atattagaac ctgataaatt gtttaatcca tctaataaca aatgatttaa
     9001 ccaagatgct ggtggattaa agacataaat catcaataag cctgtagctg tcactgtcaa
     9061 tagtgggtaa attaatgttg gttttaatcc ctctaatgct tgtggtaact tacgtgtcat
     9121 cgctttaaca ccttgtgtta agtaacctgc taagaaacct gcaataatac caccaataaa
     9181 tcctgaacca cctgaaattg ctaacatacc acctacaaga ccagctgcaa aaccaggttt
     9241 atccgcaata ctacgtgcaa tgaatccaga taaaattgga atgattaacg cgaatgcact
     9301 tttactacca atgttccata gctgctctgc aaacgcattg tactctgagc tttttggttt
     9361 aaatgagtct actccaaata aaaatacgat tgccattaaa ataccaccag atattacaag
     9421 tggcaacatg ttagaaacac cgttcattaa gtgtttatag aatgctttac ctgggcttaa
     9481 tttttcttga ctgtcatttg agttaccttt tctttgacca tcacgggcaa caaaaggttt
     9541 acgacttgta tctaatgctt tattaattaa ttcttctggg cgtttaatac catctgctac
     9601 tggtacttct acgacatttt taccatcgaa gcgatccgtc tcaacatgaa catcagcagc
     9661 aacaatgata cctgttgcat tttcaatatc ttgttcagtt aaatggtttt taatgccgct
     9721 tgaaccattc gtttctactt taattttaat acccatctta tccgcttgtt ttttcaatgc
     9781 gtcacttgcc atgtacgtat gtgcaatacc tgttggacaa gcagttactg ctaacacata
     9841 tggctcatta gattgtttag atgattgttc tgcagttgca acttgttgtg cttcagcttc
     9901 tgcctcttct tcttttgtca cttcatcatc agcctcatct atgatcgcta gtacttcttc
     9961 aggtgatgaa gcatgtaata atttctcacg tacattttca tccattaaaa taccagacaa
    10021 cttagctaaa gcatctagat gtgtttgggc gccaccttct ggcgctgcaa tcataaagaa
    10081 taagtgtgct ggttgcatat ccaaactttg ataatctacg cctgctttag atttaccaaa
    10141 cgcaatagct ggtgacttaa ctgcggccac tttggcatgt ggaatggcaa taccttcgcc
    10201 gataccagtt gtactttgtg attctcgatt gtgaatcgct tccttaaatg acgcgacatc
    10261 acttaattta cctgctttgt ctaattgatt tactaactca tcaataacac catttttgtc
    10321 atttgccatt aaatccattg ctattgtatc ttttgttaat aactctgtta ctctcattat
    10381 tttcactccc catcaagtac gctaatcgta acttgtgatt ttattttttc tatagcgtcc
    10441 cgtgttgcta agtcctcatc aaatgccgtg gcagtaccgc atgcgactgc ttgttggaat
    10501 gctttttcaa tcgttaaacc tgaagcaatt ccagccacca tgcctgcaac tgtactatca
    10561 ccagagccaa ctgtattaac cactttccct tgtggattaa ctgctttaat actgatttct
    10621 ttatcaatat aaatagcacc atcaccgcca agcgagacaa taacagattg cgcaccttta
    10681 tcaactaaca aacgaccata tttaataaca tctgtgtctg agttcactgt tgtattaaac
    10741 atcacttcta attcatcttt attaggttta ataaatagtg gatgatatgg taaaacgctt
    10801 tcagccaatt ctttttcagc gtcgactact aatttagcac ctgtctgtgc tgtaatttgt
    10861 gcaatttgcg catacgcatc gcttggaata ctacttggta cacttccagc aacaataact
    10921 atatcttcgc ttgttgtatt tttaatttgt tgtaacagtt gttcaaattg tgttgacgtt
    10981 atatgaggac ccggtgcatt gatttctgtt tcttgtcctg tttttaattt cacattaata
    11041 cgtgtatctt catcaacttc aataaaattc gattgaattg cactgttatt taatgtatct
    11101 ataatgaatt tcccaggaaa tccacctgca aatcccaagg cagttgactc aacatccaat
    11161 gtctttaaga cgcgcgagac attaatacct ttccccccag cgaatttata tgttgctgtt
    11221 gctctgttca aaccatcaat tttaaaatca ttcgtaaaaa tgacatagtc aattgaagga
    11281 ttgaaagtca ctgtataaat cataaagtcc ctcctataaa gtgatacttt tgttggtatt
    11341 cttttaacga ttcttgattt aatgcttttt cagatgtgat gattgtcgta ctttctagca
    11401 aaggtacacg agcaaaatat actttattaa acttagaatg atctataagt acaaatgatt
    11461 gattggctaa tgacattgct gtttgtttaa ctaatgcctc ttgctcatcg ggagtagtta
    11521 atccaagttc aatatctaat ccattcatcc cgataaaagc tttatcgaaa caatatcgtc
    11581 ttaatatctc catagcacta gaaccaatcg tagcaagtgt attttcttta acttgaccac
    11641 ctagcataat tgttttaata ccttttttaa gtaaagcttc tacatgtgtt aaaccattgg
    11701 ttaccacaat gatatctttc gcttgaatat atttaattag ctccaatgta gatgaaccag
    11761 catcgataaa taagcattca ttatcgttga tttgattagc tgctatttta gcaatcattt
    11821 tcttttcatc aagattcgtt gctaattttt cagttaaatt cgcctcaacc atacgatttt
    11881 cttttaacat tgcaccacca tgcacacgtt gcaatttccc taattgttgt agtttagata
    11941 aatctcttcg tattgttgaa gcactgcatc cagttcgatc tattagttct tgtaaagtca
    12001 aaaaatcttt gtgcgaaagt tcttctaata ttaactcgtg tcttttttct gtaattatca
    12061 tcatcacccc atctcactct gtattataat gagtacgctt tcacttttca atcataaatt
    12121 accaaaaaca atcacaaaca atcaaattgt gacgataaaa ttacattttt atgttcaaat
    12181 actcatttgt aatgactgtt tatgatcgta aaatgctaat tcaatcaaaa aggagtgtgg
    12241 cagaaatgaa ttttataaaa atttcatctc tgtcacactc cgaatcttgc cccatacttt
    12301 ttgttgttat taatcattca tggataactg ctccaatttt gcctttagtt attgtaatca
    12361 aatcctcaac agctattgtg atttgcattg ttcgaagccc accactcaca ctgatatgac
    12421 tataattttc acacgatttg tcaacgactg ttggaaacaa tgttttcata ccaacaggcg
    12481 aacacccacc acgaatgtat cccgttacat ttttcaaatt atctaaaggc ataagctgca
    12541 atttcttctc tccaaccaaa gcagctgcct ttttcatatc taaagtttca cttactggga
    12601 taacaaatac aaaatgttca tgttttgtat tttctagaac aagcgtttta aatactgttt
    12661 taacatttgc ttgtatgagt tctgcgactt cttgaccaga taaatgttct tctggtacct
    12721 caaagctatg aacttcgtat ttaatttttg cacgatcaag catacgcatc gcgttcgttt
    12781 tcttattttt agccatttca acacctcatt tatacttctt cagaactata ttaacatttt
    12841 aaaatacatt acggattggt tgattttgac ataacattta catcatttta taagttgatt
    12901 tcaccttttt aaattaaatt attgcaaaat aaaaacagta tatccaatta gaatacactg
    12961 ttccattatt atttcgttat tatgccttga tttgaaatac ttacatgttg cttactagtt
    13021 gaattatcat tggaataaac ataattatat ccatgatttg tcttagaaat tctttctaaa
    13081 ccataattat cattgatatg cttatctgca taacgatcag caataacttg gcactcttgt
    13141 tcgttaaaag aatttgattt atttgcattc acacgatgtt taaaatgatt ttgctttttc
    13201 atattttcat tcacttcaga tgctttagca gtatgaatat tgagaatatc cattttcttt
    13261 ttataagtat cttcagttga tacatttatc gcgttaagcg tattaattgt tttatgtacc
    13321 ttagcaagcg catcttcatt tgttgctaat attaataaac caattaaaca aacggttaat
    13381 attacagcaa caaagcctaa aaataaattt tttactgtca tccccttacc cacatttcct
    13441 tactcatttt ttatcatctt aacacattaa cgttaatgaa ttaggtatgt ggattgcaat
    13501 ttaaaaagaa tgtaatattt gtaacatgtt tattactcaa aatcattttt tgttacatat
    13561 aacatttaaa tataaagagt aaaacattta gtcaatcgaa tctttttaaa tgtaaagcat
    13621 cgaattttct taataaaatt cataagaaaa acgatgctaa tcattcataa tattacgctt
    13681 tactttatat caatatacac caaaatactt atgctacata ttttgttctt tcaattttgc
    13741 tctatgttgc ttttcaatta aaacaataac aacacctgct aatgaaacac ctatagccat
    13801 ataaattggt gcttcaatgt gtacatcaaa taacgcacct gcgattaaag gacctatgaa
    13861 attacccata ctagtgaatg tcgagttcaa tccgcctgca aagccttgcc tttctccagc
    13921 aatattagaa aaataatttg taatggctgg tcgtatcata tcaaaaccta tgaagacaac
    13981 aaaactgatt aacattattg accaatagcc attagcaaaa actaataata ttaagacaac
    14041 aactgaatat aataatgacc aagctataaa tgttaactct gagaaatact tcataaattt
    14101 atcgaagaaa tagatttgga aaagtgcccc aaatataccg ccacccgtaa tagcaatcga
    14161 aatatcttta ggtgaataat ttaccttgtc agctgtgtat agtgaataca atgtttcaaa
    14221 tgcagataaa ccaaacgata atacaagtgt taaaataact ggtgtaataa acactttcca
    14281 gttaattttc gttagcaatt gtggctctaa cttttggaaa ccacttgtcg tagacttttt
    14341 cggatcgtga atcaatacaa ttgacattat aaatgctaga atacctaatg ctcctgcaaa
    14401 gtaaaatggc atacgatgtg aaacttctgc cataaatcca ccaatccctg gtcctaaaat
    14461 gaatccagaa ttgataatcg ctgacatgta gccaaagttt tttgcttttt gatggcttgg
    14521 tgaaatgtca gctattaaac ctgtcacacc aggcattacc ataccagcac tcataccacc
    14581 aatcactctc gataacatca ataccgaaaa attgtggcca actgcaaaca taaattctga
    14641 cactgaaaac aaaattaatc ctatacatat aattaatttc ttccctaatt tgtcagctag
    14701 cgtaccacca aacggcgata taatcatttg agataacgca aaagcagcaa ctagtaatcc
    14761 taaatcacta ccagttaatc ccaaatcttt taaataaaca ggcaagactg gtattactaa
    14821 accgatacct aaaaaaatca agaaaatatt aaaatataag acaaaaatct gtttattcat
    14881 atgctcacct cttttttctc catgtcatgc ttaaagctgt tattcttctt ttccacgaca
    14941 gattgcaatt catgctaaat attcgtaaaa agtttctata ttgtattaca acattgctac
    15001 atttgacaat attttttctt tgtaattatc actattttcc atttaattgt ataattaatg
    15061 acatattaaa ataaagggga ttgatcatat gaaattgcaa aaaagtaatc atactatact
    15121 actagtgttg gaaaaaggag aagatatcgt tgaatgtata acaacctttg cagatgacca
    15181 agacttaaca tttacatctg tcagcggcat tggtgcatgt gatgacgttg tattaaaatt
    15241 tttcaattta acaacgaaac aatacgaaga aaaacatatt actgagccac tggaattgac
    15301 gagcttatta ggtaatattt ctcgtctaga caatggtcat tttgcacatt tgcatgctac
    15361 tttcggtact caatcatatg aaacttttag cggacatctc gctaaagcaa ttgtttctgc
    15421 aactgctgaa atcattctta ctgtaactga tttggacatt caacgttcat tcaaagatgc
    15481 agtcggttta aatttacttg atcctcaata gcactttact ttttagttga atcaaatttt
    15541 attacaagtt atcataagtg cttaatcata aatgcattct cgtttattaa tatacttcaa
    15601 cgagttaaat taaattttta attaggaatt gaatgcgttt ataactatca catttgaatg
    15661 aataactttt tcattagaaa gtagtgttta ttattgatgc aatctcgata catcgctaga
    15721 atttactttt taattttatt tatcgtctcc ctttttgaga ctaaaatttt tcaatttatg
    15781 actttaaatt tgtttttagc atatatacct tttgagctta gtctattatt acgattattc
    15841 aaaccgaata aaaagtatga atggccactc tttatcatat atagttttat atttgtgtta
    15901 cttttaccaa atacatttta tatggtaact gatttaatcc atttgaacca atttcaattc
    15961 aacttttacg caggactaaa tttaagtgag tggaaatact ttacctattt actgcttggt
    16021 gtcttcttag cggtttatgt gatgatttta atctattttg aaatactaac attgacttct
    16081 caccgttggc ttaatagatt aattgttgtc gtgctaatgt ttttaaatgg attcggaatt
    16141 tatattggtc gtttcttacg cttacattcc gtttatttct ttaatgagcc cttacatgtt
    16201 ctaaatcaca ttctagaaag tctaacgctt aaaactgcta tgtttgtatg ttttatggtt
    16261 gtcatgcaag cagcaattct attatttgga aaaggagtgc gtttgaacaa gtgacaataa
    16321 atataatcgt tttaatagta tctatcattg tctttcaatt gattattgga cacatatggc
    16381 atgatattgg tttaagttat ttaagaagta tcttactcat gatgctgcca tttggactag
    16441 gtgtatttat acaacaagtg tcgtattacg aacgacaata tcctaaatgg caagtgcctc
    16501 aaaacataaa agttagattg aaatatattt atctcgctac gtttttagag tatgtcgtac
    16561 tatacctcac tttatttacg gacatactaa gataacatca aattaataag aaaactaaat
    16621 acaacttata tatttgagga aagccaattt ctatcacatc aataatagaa attggcttat
    16681 ttatattgct taagaacttt atattctgct ccagatttta ctcatgccag atctatcaat
    16741 tgagccgaat tagtctagcg ctttaaatgt agctaaaact tgtgtacgtt tttcttgatg
    16801 atctaccatt tgtcttggat aatgactacc taattcaatc ccctgttcaa aaaggttgga
    16861 ctcattgcgt tgtgtatcat gcaaatattt tgcatcaatt tgattaaaaa tcggaagata
    16921 tgttttgata tacaaagctt tagcatcaaa gcgttcactc tgtcttattg gattaaacat
    16981 tctaaaatac ggcactgcat ccgtacctgt agaagcagac cattgccatc cgtgaatatt
    17041 tgatgctgca tcatagtcaa taaggtgctt tctaaagaat ttttctcccc atgtccaatc
    17101 tataaataaa tctttggtta aaaattgcga cacaaccatt ctcattcgat tatgcataaa
    17161 accagtttgt gtcaattcca ttattgctgc atcaatgatt ggaaatcctg tttgcccttc
    17221 gcaccatgca ttaaaatccg cttcattttg cgaccatttt atctgtcgat atttaggttt
    17281 gaaagcttgg tatgaggttt caggatactg tgtcattaac acataataaa attctctaaa
    17341 aatgagttca cgtataaatg cttcatagtt tgcctcatct tcatcataac gcgccaataa
    17401 atcattaaaa atttcaataa tatctaataa tccatatgct aaagcaacac ttagctgact
    17461 tgttaatact tcaggtaaat agtctcttcc gctttcgtaa ttctgtatat cttgatctaa
    17521 aaaatgttgc catttattct gttcaattat ttgtgaggat ccaaatgcta tgtcatcaaa
    17581 cactaaatca tcaggtgatg caatgacaaa gtcttcgaat gattttaaat catagtgata
    17641 tacgtctcta tgcctcaagt aaggtcgcca ttttttatag aaactagtaa aaactttgta
    17701 tggctcccct tgttgattca ttgttttact cgctttaaag taatgattcc cacgtatcaa
    17761 agtaacggca atgcgtgctt cattgaataa agaacgttga tgtaaaatat catattcttc
    17821 aagatgataa ctcataatat cacccgcagt cactacttca gagatattgt tttctttaca
    17881 aaatgaaatg atatcgtcat aagacatgac ataaggtgtg agcttttctg cgtgcaacga
    17941 ttggtaaaag cgctgcaaag taccgcgata aaactgtgct ttcaaatcag aagctgaaga
    18001 catgtcctct tccggaatga taaaataaca tgcatcaatg tcttcttttt gttgataaat
    18061 atattcaaat aatggattgt gttccattcg aaacattcga tttaataaca cagcaattgc
    18121 cataaacaaa acacccttca attatctttt tattttatta agctttttgt cactttaaaa
    18181 taacctagat tcttatttat atttttaatt aaagcaaacc taaccatttc caataagtca
    18241 tgctaaaaac tatcactaga atatacccta caattgtcaa tggtatacct gccttcaaga
    18301 aatctttaac cgtaaaagta ccagtgccat aagccaacat attttgaggt gcactaacag
    18361 gtaataagaa accaaaacta ataacaaatt gttgaattaa aacaaatcct atagactggt
    18421 ctcctaagtg taacgtagag gttagcgaaa taaaaacagg tattaacgct gatgataaac
    18481 ttgttgcact cgcaaagccc aaatgaatca atatattaaa aagcgtgata agtgcaattg
    18541 tcgcgataat aggtaaatgt tttaaaccta aaacaccaaa agtttgatca cttaaccatt
    18601 gagctgcacc tgttttcaaa agaacgttcc ctagtgaaat acctacacca aacacgataa
    18661 ttgttcccca tggtatttta ttatcaacat gtttccatgt catgacacca attttcggca
    18721 ttaacataac acctaaagca ataatagtaa tggatgcaga gtcaatcgga tgtaatactt
    18781 tttcagttga ccaaaacagt aataataaca tcgatataac aattaaacgc cattcacgtg
    18841 ggctaacggg gccaagttta tgcaattctt cttttattaa atctttacca ccttctattg
    18901 tattaatttc tggaggcatc actttaatca tgatgaaata taaagctacg gacataacta
    18961 tggaccaagg cgctgcatat aagaaccact cgccccatga aacatcaaat cctaattgat
    19021 ggtttataaa attaatcgct acgatatttt gtgctgccgc cgttttgata ccaatattcc
    19081 aaattgacac agcttgtact gaagtaatta ttaataaaga cgctaacttg ctatctttgg
    19141 aaactttaaa tgccgcaatc atacccagca agattggtac aactgcccct gctctagctg
    19201 ttgcagaagg aacgaaaaat gcaagtacaa ttgaaacgat aattgctcca ataactatat
    19261 ttctagtttt attaccaaca attgataaca ctaaaagagc tagtctttta tgcaaattcg
    19321 tttcttgcat agcagcagcc aaaaataatg cagctgcaac gagagctaca gctgaagttg
    19381 catatccact aaacgctaat gataatgcat gattagttcc tagaaggtca cttccagcta
    19441 aaatagcact gccacttttc ggattaccta gcttctcccc taaattttga accgggctaa
    19501 atcctaaaag taatatcatt aagccaataa ttaaagttgc tgtcaccgga tatgatacag
    19561 cttccgttac ccacataata actgcaaaag ctaaaatagc tagtactgcc ttagccatta
    19621 tcggcagact tgaaggtgcc gggagtaata gtacaactat aagtattata aaactcatga
    19681 caatccaaaa tggcttgtat tgcttttttg tcttttcttt cttttgttgt tcatgttcca
    19741 ctacatgttt ttcttctgac ataacagtca cccctttgtt taatcttaat ttaattataa
    19801 caatttttta aaacat
//
LOCUS       DAMZKC010000037        19804 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.037, whole genome shotgun sequence.
ACCESSION   DAMZKC010000037 DAMZKC010000000
VERSION     DAMZKC010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 19804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19804
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.037"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(23..1192)
                     /locus_tag="POB55_000973"
     CDS             complement(23..1192)
                     /locus_tag="POB55_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HDI6499947.1"
                     /translation="MIKNTIKKLIEHSIYTTFKLLSKLPNKNLIYFESFHGKQYSDNP
                     KALYEYLTEHSDAQLIWGVKKGYEHIFQQHNVPYVTKFSMKWFLAMPRAKAWMINTRT
                     PDWLYKSPRTTYLQTWHGTPLKKIGLDISNVKMLGTNTQNYQDGFKKESQRWDYLVSP
                     NPYSTSIFQHAFHVSRDRILETGYPRNDKLSHKRNDTEYINGIKTRLNIPLDKKVIMY
                     APTWRDDEAIREGSYQFNVNFDIEALRQALDDDYVILLRMHYLVVTRIDEHDDFVKDV
                     SDYEDISDLYLISDALVTDYSSVMFDFGVLKRPQIFYAYDLDKYGDELRGFYMDYKKE
                     LPGPIVENQTALIDALKQIDETANEYIEARTVFYQKFCSLEDGHASQRICQTIFK"
     gene            complement(2218..3912)
                     /locus_tag="POB55_000974"
     CDS             complement(2218..3912)
                     /locus_tag="POB55_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HDI6499948.1"
                     /translation="MTKTKQAIHIDNIYWERVQLYIEGHSEGVDLTSGQFVLRNLTET
                     KTLEANEMKIDGNTFICRFNVAILDDGYYLPMDKYLFVYHDQLEYIGQLNPNIIDQAY
                     AALNEEQIEEYNELTTQNGKVNYLLAYDAKVFRKGGVSQHTVYTITPEIASDVNEFVF
                     DIEITLPQEKSGVIATSAHWIHKQGHKASFESRSFLFKAIFNITKLLHIKRSKTILFT
                     SDSRPNLSGNFKYVYDELLRQKVDFDYDIKTVFKANITDRRKWRDKFRLPYLLGKADY
                     IFVDDFHPLIYTVRFRPSQEIIQVWHAVGAFKTVGFSRTGKKGGPFIDSLNHRSYTKA
                     YVSSETDIPFYAEAFGIREENVVPTGVPRTDVLFDEAYATQIKQEMEDELPIIKGKKV
                     ILFAPTFRGNGHGTAHYPFFKIDFERLARYCEKHNAVVLFKMHPFVKNRLNISREHRQ
                     YFIDVSDHREVNDILFVTDLLISDYSSLIYEYAVFKKPMIFYAFDLEDYITTRDFYEP
                     YESFVPGKIVQSFDALMDALDNEDYEVEKVVPFLDKHFKYQDGRSSERLVKDLFRR"
     gene            complement(3934..4959)
                     /locus_tag="POB55_000975"
     CDS             complement(3934..4959)
                     /locus_tag="POB55_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HDI6499949.1"
                     /translation="MINQVYQLVAPRQFDVTYNNVDIYGNHVIVRPLYLSICAADQRY
                     YTGRRDENVLRKKLPMSLVHEAVGEVVFDSKGVFEKGTKVVMVPNTPTEKHDVIAENY
                     LPSSYFRSSGYDGFMQDYVVMAHDRIVPLPNAIDLSTISYTELVSVSYHAIQRFERKS
                     IPLKTSFGIWGDGNLGYITAILLRKLYPEAKIYVFGKTDYKLSHFSFVDDIFTVNQIP
                     DDLKINHAFECVGGKGSQVALQQIVEHISPEGSIALLGVSELPVEVNTRLVLEKGLTL
                     IGSSRSGSKDFEQVVDLYRKYPDIVEKLALLKGHEINVCTMQDIVQAFEMDLSTSWGK
                     TVLKWTI"
     gene            complement(4952..5668)
                     /locus_tag="POB55_000976"
     CDS             complement(4952..5668)
                     /locus_tag="POB55_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HDI6499950.1"
                     /translation="MIYAGILAGGIGSRMGNVPLPKQFLDIDNKPILIHTIEKFILVS
                     EFNEIIIATPAQWISHTQDILKKYNITDQRVKVVAGGTDRNETIMNIIDYIRNVNGIN
                     NDDVIVTHDAVRPFLTQRIIKENIEVAEKYGAVDTVIEAIDTIVMSKDKQNIHSIPVR
                     NEMYQGQTPQSFNIKLLQDSYRALSSAQKEILSDACKIIVESGHPVKLVRGELYNIKV
                     TTPYDLKVANAIIQGDIADD"
     gene            complement(6196..7239)
                     /locus_tag="POB55_000977"
     CDS             complement(6196..7239)
                     /locus_tag="POB55_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDI6499951.1"
                     /translation="MKALKLYGVEDLRYEDNEKPVIESANDVIVKVRATGICGSDTSR
                     YKKMGPYIKGMPFGHEFSGVVDAIGSDVTHVNVGDKVTGCPAIPCYQCEYCLKGEYAR
                     CEKLFVIGSYEPGSFAEYVKLPAQNVLKVPDNVDYIEAAMVEPSAVVAHGFYKSNIQP
                     GMTVAVMGCGSIGLLAIQWARIFGAAHIIAIDIDAHKLDIATSLGAHQTINSKEENLE
                     KFIENHYANQIDLAIESSGAKVTIGQILTLPKKGGEVVLLGIPYDDIEIDRVHFEKIL
                     RNELTVCGSWNCLSSNFPGKEWTATLHYMKTKDINVKPIISHFLPLEKGPETFDKLVN
                     KKERFDKVMFTIY"
     gene            complement(7263..7409)
                     /locus_tag="POB55_000978"
     CDS             complement(7263..7409)
                     /locus_tag="POB55_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499952.1"
                     /translation="MVESMLTFMLGPLRQITDFYMEHLLVSNSIVIAGYFATGIFKKK
                     KVVN"
     gene            complement(7411..8466)
                     /locus_tag="POB55_000979"
     CDS             complement(7411..8466)
                     /locus_tag="POB55_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HDI6499953.1"
                     /translation="MKALVKTREGHGNLELLDKEVATPLDDKVKIKVHYAGICGTDIH
                     TYEGHYKVNFPVTLGHEFSGEIVEVGADVKDFKVGDRVTSETTFYVCNECEYCKSKDY
                     NLCNHRKGIGTQVDGAFTNYVIAREESLHHIPDEVSYQSAAMTEPLACAHHGVSKIQV
                     NSGDVAVVMGPGPIGLLVAQVLKSKGATVVVTGLDNDKVRLDKAEALHMDYVVNLQQT
                     DLKTYINGITDGYGADVVVECSGAVPAARQGLDILRKKGFYSQIGIFKDAEIPFDMEK
                     VIQKEITVVGSRSQKPADWEPSLQLMADGLVNAEALVTKIYDISKWDEAYQHLKSGEG
                     IKALLKPLDLDENEGEN"
     gene            complement(8484..9743)
                     /locus_tag="POB55_000980"
     CDS             complement(8484..9743)
                     /locus_tag="POB55_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDI6499954.1"
                     /translation="MSYFTDFVRGFLDLGATVILPVVIFLLGLFFRQKIGAAFRSGLT
                     IGVAFVGIFLVIDLLVKNLGPAAQAMVKNLGVSLNVIDVGWPATSSIAWASSVAAFII
                     PLGIIVNVVLLVTKVTKTMNVDIWNFWHYTFTAAMVYAVSGSIWQALLAAVIFQVICL
                     KVADWTAPMMSEFFDLPGVSIATGSTISYAPGIYLVKLLQKVPGLNKLDADPETIQKR
                     FGAFGESIFVGLILGLGIGVLAGYKPGDIINLGMSMAAVMVLMPRMVKILMEGLMPVS
                     ESARTWLNKRFGEREIYIGLDAAVALGHPAVISTALILVPITVLLAVILPGNQVLPFG
                     DLATIPFVVAFIVGAARGNIIHSVIVGTIMIAISLYIATDVAPIFTDMAKGTNVQMPK
                     GSSEISSIDQGGNIVNYLIFKLFSLFN"
     gene            complement(9970..10248)
                     /locus_tag="POB55_000981"
     CDS             complement(9970..10248)
                     /locus_tag="POB55_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDI6499955.1"
                     /translation="MKQVLVACGAGIATSTVVNNAIEEMAKEHNIKVDIKQIKITEVG
                     PYEDTADLLVTTAMTKKEYKFPVINARNFLTGIGIEETKQQILTELQK"
     gene            complement(10271..10738)
                     /locus_tag="POB55_000982"
     CDS             complement(10271..10738)
                     /locus_tag="POB55_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDI6499956.1"
                     /translation="MGQQLVHKENIMLNLSATDKESVLSQMSDVLFQNGFVKSTFKDA
                     VIDREKEFATGLPTHLCSVAIPHTDVEHINHRTIGVAVLEKEVPFIEMGTLDQQTEVK
                     IVFMLAMDKVDDQLKLLQQLMQIFQSEEKLEQILRTKDETILATLINDYLEYN"
     gene            complement(10723..12819)
                     /locus_tag="POB55_000983"
     CDS             complement(10723..12819)
                     /locus_tag="POB55_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDI6499957.1"
                     /translation="MNGDNQQILREIVLNPTIHGKELESIFGLSRRQLGYRIQKINLW
                     LEQEGYPKLERTSQGNFIVSSEIMTLFKRDVSEQQMLNGNNVIFSIETRRYYLMLMLF
                     SKENAMSLNHFSIDLQVSKNTVIHDINHVKEQLENHGLSLKYSRKHGYEIVGDEFEVR
                     RFFIKLIDQRLNHDITKSEVLKALNLTFEDIAYQKDKIKQVEQFLKSRFIDKSLSSLP
                     YVLCVIRRRIQSGHVMNPLNINYQYLRDTKEYQATEIMTQLEPDLPEAEKLYLTLHLL
                     STSVQWTDLQESDNISNLTMAIAQMIHHFEQITFINIEDKEKLSQQLLLHLTPAFYRI
                     KYNLTDRDELINPLQGNYQSLFHMVKQSCQSLTEYFGKSLPDNEIAYLTMLFGGSLRR
                     QDENFDGKIKAIIVCTQGTSVSQMMLYELRNLFPEIIFLDAISLRTFENYTLDYDIVF
                     SPMFVLTHKKLFITKVALSENEQRKLRKEVMKYINKESADIDKEINKLMALIERTTTV
                     NDITELRDGLEDFIANYNSISTINGSIVTQNKTLDLADLIPARHVKRIHHVENIDEAI
                     AKASDVLVANHFIDIKYIHEMQQVFDDSYMVIMQNIAIPHAYSEKHVHKTAMSMLILQ
                     EPIYMSDGTAIHIIVPIAAVDKVTHLRALLQLRDVAQDNDAIKRIIQSRKNSDVNEIL
                     KNYSNKEARENGWDSN"
     gene            complement(12950..13046)
                     /locus_tag="POB55_000984"
                     /pseudo
     CDS             complement(12950..13046)
                     /locus_tag="POB55_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498807.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13156..14091)
                     /locus_tag="POB55_000985"
     CDS             complement(13156..14091)
                     /locus_tag="POB55_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HDI6499958.1"
                     /translation="MKRKIIMDCDPGHDDAIALILAGAIDSPLEILAVTTVAGNQSVD
                     KNTTNALNVLDIMGRQDIAVAKGADRPLIKPAAFASEIHGESGLDGPKLPSTPSRQAV
                     AMPASDVIINKVMTSDTPVTIVATGPLTNVATALIREPRIAEHIESITLMGGGTFGNW
                     TPTAEFNIWVDAEAAKRVFESGITINVFGLDVTHQVLADDHVIERFESINNPVAQFVV
                     ELLQFFKKTYKTHFNMDGGPIHDACTILYLLQPELFTMVPVNIDIEHQSPLTYGTMAV
                     DLNHVTGKPANAYFATAVDVEEVWNLIDHKLRTYE"
     gene            14453..15982
                     /locus_tag="POB55_000986"
     CDS             14453..15982
                     /locus_tag="POB55_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDI6499959.1"
                     /translation="MKSLFEKAQQFGKSFMLPIAILPAAGLLLGIGGALSNPNTVKAY
                     PILDITLLQNIFTLMSAAGSIVFQNLPVIFAIGVAIGLSRSDKGTAGLAALLGFLIMN
                     ATMNGLLTITGTLAKDQLAQNGQGMVLGIQTVETGVFGGIITGIMTAILHNKYHKVVL
                     PPYLGFFGGSRFVPIVTAFAAIFLGVLMFFIWPSIQAGIYHVGGFVTKTGAIGTFVYG
                     FILRLLGPLGLHHIFYLPFWQTALGGTLEVKGHLVQGTQNIFFAQLGDPDVTKYYSGV
                     SRFMSGRFITMMFGLCGAALAIYHTAKPEHKKVVGGLMLSAALTSFLTGITEPLEFSF
                     LFVAPILYVIHAFFDGLAFMMADIFNITIGQTFSGGFIDFLLFGVLQGNSKTNYLYVI
                     PIGIVWFCLYYIVFRFLITKFNFKTPGREDKAAAQQVEATERAQTIVAGLGGKDNIEI
                     VDCCATRLRVTLHQNDKVDKVLLESTGAKGVIQQGTGVQVIYGPHVTVIKNEIEELLG
                     D"
     gene            complement(16302..17255)
                     /locus_tag="POB55_000987"
     CDS             complement(16302..17255)
                     /locus_tag="POB55_000987"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDI6499960.1"
                     /translation="MNKFKGNKVVLIGNGAVGSSYAFSLVNQSIVDELVIIDLDTEKV
                     RGDVMDLKHATPYSPTTVRVKAGEYSDCHDADLVVICAGAAQKPGETRLDLVSKNLKI
                     FKSIVGEVMASKFDGIFLVATNPVDILAYATWKFSGLPKERVIGSGTILDSARFRLLL
                     SEAFDVAPRSVDAQIIGEHGDTELPVWSHANIAGQPLKTLLEQRPEGKAQIEQIFVQT
                     RDAAYDIIQAKGATYYGVAMGLARITEAIFRNEDAVLTVSALLEGEYDEEDVYIGVPA
                     VINRNGIRNVVEIPLNDEEQSKFAHSAKTLKDIMAEAEELK"
     gene            complement(17566..17673)
                     /locus_tag="POB55_000988"
     CDS             complement(17566..17673)
                     /locus_tag="POB55_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499961.1"
                     /translation="MSVCTPTIARIYLTNSKNIVVFLEKSKHDNKNVKV"
     gene            17828..18973
                     /locus_tag="POB55_000989"
     CDS             17828..18973
                     /locus_tag="POB55_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide dioxygenase"
                     /protein_id="HDI6499962.1"
                     /translation="MLTEQEKDIIKQTVPLLKEKGTEITSIFYPKMFKAHPELLNMFN
                     QTNQKRGMQSSALAQAVMAAAVNIDNLSVIKPVIMPVAYKHCALQVYAEHYPIVGENL
                     LKAIQDVTGLEENDPVIQAWAKAYGVIADVFIQIEKEIYDQMMWIGFKPFKITNIKQE
                     SEDIKSFTVETEEYDFSEFTPGQYITVDVSSEKLPYRAKRHYSIVSGEKNHLTFGVKR
                     DVTTEHEGEVSTILHDEIKEGDMINLAAPVGGFVLENTTEPQLFLGSGIGVTPLVAMY
                     EAASAKGLDTQMVQVAENEQHLPFKDNFNSIASHHDNAKLYTHLKDKQGYIGAEELQA
                     FLANKPEIYICGGTKFLQSMIEALKSLNYDMDRVHYETFIPRLSVAV"
     gene            18999..19172
                     /locus_tag="POB55_000990"
     CDS             18999..19172
                     /locus_tag="POB55_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6499963.1"
                     /translation="MKLNQRYVKVFALYFVSIVTANIIVKNNNLIKTLIQTIAGYTVF
                     AVGLKYLTKRKNK"
     gene            19329..19685
                     /locus_tag="POB55_000991"
     CDS             19329..19685
                     /locus_tag="POB55_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HDI6499964.1"
                     /translation="MTVDIGRIYDNKDNTDAIRILVDRVWPRGISKRTANLDYWLKDI
                     APSTELRQWFQHDPKLFGAFKEKYEKELRDQDAQKDAFEKLKDIVNQHNHVLLLYAAK
                     DTKHNQAVVLQQLLNT"
BASE COUNT         6197 a         3786 c         3052 g         6769 t
ORIGIN      
        1 attttttatt gtttttaagt tatcacttaa aaatcgtttg gcaaattcgt tgtgacgcgt
       61 gtccatcttc taatgaacag aatttttgat aaaataccgt tcgtgcttca atatactcat
      121 ttgcagtctc atcgatttgt tttaatgcat caatgagtgc tgtttgattt tcaacaattg
      181 gacctggcaa ctctttttta taatccatgt aaaaacctct aagctcatcg ccatatttat
      241 ctaagtcata cgcatagaaa atttgcggac gctttaatac accgaagtcg aacatgacag
      301 atgagtagtc ggtaactaac gcatcgctga ttaagtataa atccgaaata tcttcataat
      361 ctgaaacgtc tttcacaaaa tcatcatgtt catcaatacg tgtcacaact aaataatgca
      421 tgcgtaataa aataacataa tcatcatcca gcgcttgacg caaagcttct atatcaaagt
      481 taacattaaa ttgatatgaa ccttctcgaa tcgcttcatc gtcacgccaa gttggcgcgt
      541 acataatcac ttttttatct aatggaatat ttaatcttgt cttaatacca ttaatatatt
      601 cagtatcatt gcgtttatgt gataatttat catttcttgg ataacctgtt tccaaaatcc
      661 tatctcgact aacatgaaat gcatgttgaa atatcgatgt cgaatatgga ttaggtgaca
      721 ctagataatc ccaccgttgg ctttcttttt taaagccatc ttggtaattt tgagtatttg
      781 ttcctagcat tttaacgtta ctaatatcca aaccaatctt tttcaatggc gtgccatgcc
      841 atgtttgtaa gtacgtcgtt cgcggtgatt tatataacca atctggtgta cgtgtgttaa
      901 tcatccacgc tttcgctctt ggcatcgcta aaaaccattt cattgaaaac tttgtaacat
      961 atggtacatt gtgctgttgg aatatgtgtt catatccttt tttcacaccc catattaatt
     1021 gggcatcgct atgttcagtt aagtattcat ataatgcttt ggggttgtcg ctgtattgtt
     1081 taccatgaaa gctttcaaaa taaattagat tcttgtttgg caattttgat agtaatttaa
     1141 aagtcgtata tatactatgt tctatcaatt ttttaattgt atttttaatc atgtcgtacc
     1201 tccgacgtgt ttttgtaatt atattaatat gtatgagcaa gctcattgta accatgccta
     1261 ttatagcatt tcatcataaa atacatttaa ccattacact tgtcgttaat tatcatacga
     1321 aatacatgat taatgtacca ctttaacata acaaaaaatc gttatccatt cataacgtat
     1381 gtgtctacac atttatgaat tagataacga ttggatcgat tattttattt tacaaaatga
     1441 caattcagtt ggaacaggct gggacataaa tcaatgttct atgctctacg aagttatatt
     1501 ggcagtagtt gactgaacga aaatgcgctt gtaacaagct tttttcaatt ctagtcaggg
     1561 gccccaacac agaagctgac gaaaagtcag cttacaataa tgtgcaagtt ggggatgggc
     1621 cccaacacat agaatttcga aaagaaattc tacaggcaat gcaagttggg gtgtgggccc
     1681 caacacagaa aattcatttt attgaatttt acatttatgt gcaagttggg caaagtatct
     1741 tatttttcaa agtatttaaa agtaaaatta catgttaata cgtagtatta atggcaagac
     1801 tcctgaggga gcagtgccag tcgaagacag gggccccaac acagaagctg acgaaaagtc
     1861 agcttacaat aatgtgcaag ttggggtggc tgagacggca ccctaggaag ggacccgtca
     1921 tcaaaaattc tatttataga attttacagt aatgtgccag acgggcaaag cgaagccatt
     1981 caatacgaag tattgtataa atagagaaca gcagtaagat aattttcaat tagaaaatat
     2041 cttactgctg ttttttaggg atttctgtcc cagcctctta tgcatgaaaa atcaaaaggt
     2101 tattctcatt gtatagtcct gcttctcatc atgacatgtt gctaacttca ttgtcagaac
     2161 ccttcttgaa aactatgcct tatgactcat ttgcatggca agtaatatat gccaacatta
     2221 gcgtctaaac aaatctttga ctaaacgttc acttgagcga ccatcttgat atttaaaatg
     2281 tttatctaag aatggtacaa ctttttcaac ctcataatct tcattgtcca aagcatccat
     2341 taatgcatca aaggattgta caattttacc tggaacaaat gattcatatg gttcatagaa
     2401 atcacgcgtc gtaatgtaat cttctaagtc aaatgcatag aaaatcatcg gctttttaaa
     2461 tactgcatat tcatatatta aagatgaata atcactaatc aacaagtctg taacaaagag
     2521 aatatcgtta acttcacgat gatctgacac atcgataaag tattgtctat gttcacgtga
     2581 aatattaagt ctatttttta cgaacggatg cattttgaat aacacaactg cattatgctt
     2641 ctcgcagtat cttgctaaac gttcaaaatc aattttaaaa aatggataat gtgccgtacc
     2701 gtgaccatta cctctaaatg tcggtgcgaa tagaataact ttcttacctt ttataattgg
     2761 caattcatct tccatctctt gtttaatttg tgttgcataa gcttcatcaa atagtacatc
     2821 agtacgtggt acacctgttg gtacaacatt ttcttctcta attccaaatg cttcagcata
     2881 aaatggaata tcggtttctg atgaaacata tgctttcgtg taactacgat ggtttaatga
     2941 atcgataaat ggaccacctt ttttacctgt acgactaaag ccaactgttt taaaagcacc
     3001 aacggcatgc cacacttgaa taatttcttg tgatggtcta aagcgaaccg tataaattaa
     3061 tggatggaaa tcatcaacaa aaatataatc tgccttacca agtaaatatg gcaatctaaa
     3121 cttgtctctc catttacgtc tatccgtaat attcgcctta aataccgttt taatatcata
     3181 atcaaaatct actttttggc gtagtaactc atcatataca tacttgaaat tccctgataa
     3241 attcggacgc gaatctgatg tgaataatat tgttttgctt cttttaatat gtagtaactt
     3301 tgtaatatta aaaatagctt taaataagaa acttctactt tcaaatgaag ctttatgacc
     3361 ttgtttatga atccagtgtg cacttgtcgc aatgacccct gatttctctt gaggtaaggt
     3421 gatttcaata tcaaatacaa attcgttaac gtcacttgct atttccggag taatggtata
     3481 gaccgtatgt tgtgatacgc cacctttacg gaaaacttta gcatcatacg ctaataaata
     3541 gttcactttt ccattttgtg tagtcagctc attgtattct tcaatttgct cttcatttaa
     3601 tgccgcataa gcttgatcaa taatatttgg attaagttgt ccaatatact ctaactggtc
     3661 atgataaaca aataaatatt tatccattgg taaataatac ccatcgtcta atattgcgac
     3721 gttgaatcta catataaatg tattaccgtc tattttcatt tcatttgctt ctaatgtttt
     3781 tgtttcggtt aaattcctca gaacaaattg tcctgatgtt aaatcgacac cttcactatg
     3841 tccttcaata tataactgaa cacgttccca gtatatgtta tcaatatgta ttgcttgttt
     3901 cgtttttgtc attgttattc ccccatcggt ttattaaatc gtccatttca atactgtttt
     3961 tccccaagat gtcgataaat ccatttcaaa cgcttggacg atatcttgca tcgtacatac
     4021 attaatttca tgtcctttta ataaggctaa cttttcaact atgtctgggt acttacgata
     4081 taaatcaaca acttgctcaa aatctttaga gccgcttcga ctactaccaa tcaatgttaa
     4141 tcctttttca agtactaatc gtgtattcac ttccacgggt aattcactta cgcctaacaa
     4201 agcaatactg ccttctggtg aaatatgttc aactatttgt tgaagtgcaa cttgacttcc
     4261 tttacctcca acacattcaa atgcatgatt aattttaaga tcatctggta tttgatttac
     4321 tgtaaagatg tcatctacaa atgaaaaatg acttaattta tagtctgtct taccaaatac
     4381 ataaatttta gcttctgggt acaacttacg tagcaaaata gcagtaatat aacctaagtt
     4441 accatcaccc caaataccaa agctggtttt caaaggtata gatttacgtt caaatcgttg
     4501 tatagcatga taacttactg acactaactc tgtgtatgaa atcgtactca aatcaatggc
     4561 attaggcagc ggaacgatac gatcatgtgc catcacaacg tagtcttgca taaaaccatc
     4621 ataaccacta gatctaaaat aactagaggg caggtaattc tcggcaatca cgtcgtgttt
     4681 ttctgtaggt gtattcggca ccattactac tttcgtacct ttttcaaata cgcctttact
     4741 atcgaataca acttcaccaa cagcttcatg aactagtgac attggtaatt ttttgcgcag
     4801 tacattttca tctcttcgac ctgtgtaata cctttgatca gctgcacaaa tagacaagta
     4861 taaaggtctt acaatgacat gattaccata aatatcaaca ttattatatg tgacgtcgaa
     4921 ctgtctcggt gcaacgagtt gatatacttg attaatcatc ggcaatatca ccttgaataa
     4981 tggcatttgc tacttttaaa tcatacggtg ttgtcacttt aatgttgtat agttctccac
     5041 gtaccaattt aactggatgt ccagattcga caatgatttt acatgcatct gataagattt
     5101 ctttttgtgc actacttaag gcgcgataac tatcttgtaa taatttaata ttaaatgatt
     5161 gtggtgtttg gccttgatac atttcattcc ttacagggat actgtgtatg ttctgtttat
     5221 ctttagacat tacaatcgta tcaattgctt caatgactgt atctactgca ccatattttt
     5281 ctgctacttc aatattctct ttaataatac gttgagttaa aaatggtctt acggcatcat
     5341 gagttacaat cacatcatca ttattaattc catttacatt gcgaatatag tcgataatgt
     5401 tcataattgt ttcgtttcga tccgtaccac ctgcaactac tttgacacgt tgatctgtaa
     5461 tgttatattt ttttaaaata tcctgtgtat gggaaatcca ctgtgctggc gttgcgataa
     5521 taatctcatt aaattcactc actaaaatga acttctcaat tgtatggatt aaaatcggtt
     5581 tattatcaat atctaaaaat tgttttggta atggcacgtt ccccattctc gaaccaatac
     5641 ctcctgctaa aatacctgca taaatcatgt tgtcctccat tctgtcatta catcatttcc
     5701 atttatacat tactgaccta tgcccgcaca taagcctaac ctattgctca cttgcctctt
     5761 ttattaatcc aaagatagtt gtcacaatag tgtgataatt ttttataaaa atgtattttt
     5821 gtaactgacc attctaagtt gttttgccat gcagttaatc attaactctg acgatattaa
     5881 attgttaaag gtattaatgt ttactctttt tcaaattcat tattactgcc atcattttac
     5941 catatattat aataaattta tcttattaag tggctgtact tgattttcac tttaaaaatt
     6001 atcaaatatt gccatctcat tttaagtata caaaatgcaa aacaaccgat tcacaagcat
     6061 atttcacaca agtaaaccgg ctatttagca acgtatattc gaagatgagt tatttcgata
     6121 gtatctgtag accagacggc attcgcactt tcatagctat aactatacca gtgttttcgt
     6181 cctcaaaggt gcatactaat aaatcgtaaa catgacttta tcaaatcgtt ctttcttgtt
     6241 aactaattta tcaaatgtct ctgggccttt ttctaacggt aaaaaatgag aaataatagg
     6301 ctttacatta atatctttcg tcttcatata atgtaaggtt gccgtccact ctttgcccgg
     6361 aaaattactg gacaaacagt tccaagagcc acatactgtc aactcgttac gcagaatttt
     6421 ttcaaaatga acgcgatcaa tctcaatatc atcatatggt attccgagta ataccacctc
     6481 gccacctttt ttaggtagcg tcaatatttg accaatcgta actttagcac ctgatgattc
     6541 tatagctaaa tcgatttgat tggcgtaatg attttcgatg aatttctcaa gattttcttc
     6601 ttttgaattg attgtttgat gtgcgcccaa tgatgttgca atatctagtt tatgcgcatc
     6661 tatatctata gcgatgatat gtgcagcacc aaatattcgt gcccattgaa tagctaacaa
     6721 acctatactg ccacacccca ttactgcaac agtcatacca ggttgtatat tcgatttata
     6781 aaacccatgc gcaacaacgg ctgatggctc aaccattgct gcttcaatgt aatcaacatt
     6841 gtctggaacc tttaaaacat tttgcgctgg caatttgaca tattccgcga acgatccagg
     6901 ttcatatgag ccaataacga ataacttttc acatcgtgca tattcacctt ttaaacaata
     6961 ctcgcattga taacaaggta ttgctgggca acctgtcact ttgtcgccta cattaacatg
     7021 cgtaacatca cttccaatgg catctacaac acctgaaaat tcatgaccaa atggcatacc
     7081 tttaatgtat ggccccattt ttttgtatcg tgacgtgtct gaaccacata tgccagtcgc
     7141 tcgtacttta acaataacgt cattcgcact ttcaatgact ggcttttcat tatcctcata
     7201 ccgtaaatct tccacgccat ataatttcaa tgctttcact tgtaaatcac ctcaaatttg
     7261 atttaattca caactttttt ctttttaaaa atacctgtcg caaaataacc tgcaatgaca
     7321 atggaattac ttacgagtaa atgttccata taaaaatcag tgatttgtct taatggccca
     7381 agcataaaag ttagcattga ttctaccata ttaattctct ccttcatttt catctaaatc
     7441 gagcggctta agtaatgctt taataccttc gccagatttt aaatgttgat acgcctcgtc
     7501 ccatttcgaa atatcatata tttttgtcac caaagcttca gcatttacta aaccatccgc
     7561 cataagttgc aatgaaggtt cccaatctgc tggcttttga cttctactac caacaactgt
     7621 tatttctttt tgaatcactt tttccatatc aaatggaatt tcagcatcct taaaaatacc
     7681 tatttgactg tagaaacctt ttttgcgtaa aatatccaaa ccttgtcgtg ctgctggaac
     7741 tgcacctgaa cattcaacaa caacatctgc accgtaaccg tctgtaattc cattgatata
     7801 cgtttttaag tctgtttgtt gtaaattgac tacataatcc atgtgcaatg cttctgcttt
     7861 atctaatctg actttgtcat tgtccaatcc agttaccaca acagttgcgc ctttactttt
     7921 taacacttgt gctacaagta atccgattgg cccaggtccc attacaactg ctacatcgcc
     7981 tgaattgact tgaatcttag aaacgccatg atgtgcacat gctaatggtt ctgtcatagc
     8041 tgcagactga tacgatactt catctggaat atgatgcaaa ctttcttcac gtgcaatgac
     8101 ataattagta aatgcgccat caacttgtgt tccaatacct tttcgatggt tgcataaatt
     8161 atagtctttt gatttacagt attcacactc attacaaaca tagaatgtcg tttcagaagt
     8221 gacacggtca ccaactttaa aatctttaac gtctgctcca acttcaacga tttcaccaga
     8281 aaattcatga cctaatgtca ctggaaaatt aactttataa tgcccttcat aagtatgaat
     8341 gtctgtgcca caaattcctg cataatgtac tttaatcttt actttatcat ctagcggtgt
     8401 tgcaacttct ttatcaagaa gttctaagtt gccatgtcct tctcttgttt ttactaaagc
     8461 tttcaccaca aacacctcga tttttaattg aatagactaa atagtttaaa gataagatag
     8521 ttaacgatat taccaccttg atcaatactt gaaatttcag atgaaccttt tggcatttgt
     8581 acattcgtac ctttcgccat atctgtgaaa atgggtgcta cgtctgttgc aatatataat
     8641 gaaattgcaa tcataatcgt gcccacaatg acagaatgaa taatgtttcc tcttgctgca
     8701 ccaacaataa acgcgacaac aaatggtata gttgctaagt caccaaaagg tagtacttgg
     8761 tttcctggta aaataacggc taataaaaca gtgataggta ctaaaattaa tgctgtcgaa
     8821 ataaccgctg gatgacctaa tgctacagcc gcatccaatc caatataaat ttcacgttcg
     8881 ccaaaacgtt tatttagcca tgttcttgca gactctgaaa ctggcattaa accttccatt
     8941 aagattttta ccattctagg cattaatacc attactgcag ccattgacat tcctaaatta
     9001 atgatgtctc caggtttgta acctgctaac acaccaatac ctaaacctaa aattaatccg
     9061 acaaagatag actctccaaa tgcgccaaaa cgtttttgaa ttgtttcagg atcagcatct
     9121 aacttattca gaccgggtac tttttgtaac aatttaacta agtaaatacc tggtgcataa
     9181 gaaattgtac ttcctgtagc aatcgataca cctggtaaat caaagaactc actcatcatc
     9241 ggtgctgtcc aatctgctac tttcaaacag ataacttgga aaataactgc tgctaataac
     9301 gcttgccaaa tactgcctga tacggcataa accattgctg ctgtaaacgt ataatgccaa
     9361 aaattccaaa tatctacatt catcgtcttt gtcactttag ttactagcaa tacaacgtta
     9421 actatgattc cgagtggaat aataaatgct gcgacagatg atgcccaagc gatagatgat
     9481 gttgctggcc aacctacatc aatcacattc agactgacgc ctaaattttt aaccatcgct
     9541 tgtgctgctg gccctaaatt tttaactaat aaatcgatga ctaagaaaat ccctacaaaa
     9601 gccacaccta ttgttaaacc agacctaaat gccgctccaa ttttctgcct aaagaatagg
     9661 ccaagcaaga atatgacaac cggtaaaata acagttgcac ctaaatctaa aaatcccctt
     9721 acaaaatcag tgaagtaact catatttaaa ccctccctgt tatatatgca ttgtcacgat
     9781 actttccgat tgtgattaca tttgacgtta cagtcatttc aacgacaacc cttgctacat
     9841 tcgactgcag tcattttgaa ttacagtcac tgcgtttcta tgtcatcaac aatcatttgt
     9901 cgtgatagtc atttatatgc aatttgcata tattaatatg ttatcgaccc acgttacata
     9961 tcaattccgt tatttttgta actctgttaa gatttgttgt tttgtttctt caataccaat
    10021 accagttaag aaattacgtg cgttgataac tgggaattta tattctttct ttgtcattgc
    10081 agttgtaact aataaatctg cagtgtcttc ataaggtcca acttctgtaa ttttgatttg
    10141 tttaatatct actttaatat tgtgttcctt tgccatttcc tcaattgcat tatttactac
    10201 tgttgacgtt gcaatacctg caccacacgc tactaatact tgtttcattt tcaattcctc
    10261 caattaattt ttagttatat tccaaataat cattgattag tgttgctaaa atcgtttcat
    10321 ctttcgttcg cagaatctgc tccaattttt cttcactttg aaaaatttgc atcaactgtt
    10381 gtaacaactt aagttgatca tctactttat ccatcgctaa cataaaaacg attttcactt
    10441 ctgtctgttg atcaagtgtt cccatttcaa taaacggcac ctctttttct agaacagcca
    10501 cacctatcgt tctatggtta atatgttcga catctgtatg cggtatagcg accgaacata
    10561 gatgtgttgg taaaccagta gcaaattctt tttctctgtc gatgactgca tctttaaacg
    10621 ttgacttcac gaacccattt tgaaataaca catctgacat ttgtgacaat acggattctt
    10681 tatcagttgc cgacaaattg agcattatat tttctttatg cactaattgc tgtcccatcc
    10741 attttccctc gcttctttat ttgaataatt ttttaaaatc tcatttacat cagaattttt
    10801 gcgactttgt atgatgcgct taattgcgtc attgtcttgc gccacatctc tcaattgtag
    10861 taacgctctt aagtgtgtca ctttatcaac agcagcaata ggtacaataa tatggattgc
    10921 tgtgccatct gacatgtata ttggttcttg taatatcaac atactcatcg ctgttttatg
    10981 tacatgcttt tcagagtatg catgtggaat agcaatattt tgcatgataa ccatatacga
    11041 atcatcaaat acctgttgca tctcatgaat atatttaata tcaataaaat gattagcaac
    11101 taacacatca cttgctttag caatagcttc atcaatattt tcaacatgat gtattctttt
    11161 cacgtgcctt gccggtatca agtcagctaa atctaatgtc ttattttgtg tgacaatcga
    11221 tccattaatg gttgaaattg aattataatt cgcaataaaa tcttctaaac catcacgtag
    11281 ttctgtaatg tcattaactg tcgtagtgcg ttcaattaat gccattaact tgtttatttc
    11341 cttatcaatg tcagccgatt ccttattaat gtacttcatc acttctttac gtaactttcg
    11401 ttgctcattt tcagataaag ctacttttgt gataaataat tttttatgtg ttaggacaaa
    11461 cattggtgaa aagacgatgt cataatctaa tgtgtaattt tcaaatgttc taagtgaaat
    11521 cgcatctaag aaaataattt ctggaaataa gtttcgcaac tcgtataaca tcatttgtga
    11581 tactgacgtg ccttgtgtac acacgataat agcttttatc ttgccatcga agttttcatc
    11641 ttgacgtctc aaactacctc cgaacaacat ggttaaatat gctatttcat tatcaggcaa
    11701 cgattttccg aaatattcag ttaacgattg acatgattgt ttcaccatat gaaataagga
    11761 ttgataattt ccttgtaaag gatttattaa ttcatcacga tccgttaagt tatatttaat
    11821 cctataaaaa gcaggcgtta aatgtaacaa gagttgctgt gataatttct ccttatcttc
    11881 aatgttaata aaagtgattt gttcaaaatg gtgaatcatt tgagcgatag ccatcgttaa
    11941 attcgatatg ttatctgatt cctgcaaatc agtccattgc acacttgttg aaagtaagtg
    12001 taatgtcaaa tataactttt ccgcttctgg caaatccggc tcaagttgcg tcataatctc
    12061 cgttgcttga tattctttcg tatccctcaa atactgataa ttaatattta atggattcat
    12121 cacatgacca ctttgaattc gtctacgaat cacacaaagg acataaggca atgaactaag
    12181 tgatttgtct ataaagcgac tcttcaaaaa ttgttctacc tgtttgattt tgtctttttg
    12241 atatgcgata tcttcgaatg ttaagttgag cgcctttaaa acttcacttt tagtaatatc
    12301 atgattcaac ctttgatcaa tcaacttaat gaagaaacgg cgaacttcaa attcatcacc
    12361 aacaatttca taaccatgtt ttcgagaata cttaagtgac aaaccatgat tttccaattg
    12421 ctctttcaca tgatttatat cgtgaatgac agtattttta ctgacttgta aatcaattga
    12481 aaaatggttt agagacattg cgttttcctt actaaaaagc atgagcatta aataataacg
    12541 acgtgtttct atgctaaaaa tgacattgtt gccgtttaac atttgctgct ctgatacatc
    12601 tcgtttgaat aacgtcatga tttcagaact tacaataaaa tttccttggc ttgttctttc
    12661 aagttttgga taaccctctt gttcaagcca caaattgatt ttttgaatgc gatatcctag
    12721 ttgtctacga gacaaaccaa atatcgattc aagttcttta ccatgaatag taggattcaa
    12781 tacaatttct ctgagtattt gctgattatc cccgttcatg ttcgatcctc ctttgagcat
    12841 ctgagctcaa tttatcatca atcgtcacac cgatgtacac actttgaaca catattttca
    12901 aaatgagcat gtacatcatt gtgatgtttt aacaacattt caattatatc tatatttttt
    12961 gtgattttaa tcttttaaaa taaagcaatt gaaattttga atatattttt gtattttgtg
    13021 ttttttgaag catttttaac atacatatct caatcattat caaattgtca tgaccattgt
    13081 aatccaatac aaaaacccta aggacgctta tatcaggcac cttagggcta actgtatcta
    13141 tttaattaag tattattatt cgtatgtacg taacttatgg tctatcaagt tccacacttc
    13201 ttcaacatca actgctgtag caaaataagc attggcaggc ttacctgtaa catgatttaa
    13261 atcgacagcc atagtgccat aagttagtgg actttgatgt tcaatgtcga tattaacggg
    13321 taccattgta aacaattctg gttgtaacaa atacaaaatt gtacaagcat catgtattgg
    13381 accaccatcc atattaaagt gagtcttgta tgtcttctta aagaattgca ataattctac
    13441 gacgaactgt gcaacaggat tattgatact ttcaaagcgt tcaatcacgt gatcgtcggc
    13501 taaaacttga tgtgttacat ctaaaccaaa cacatttata gtaatcccac tttcaaaaac
    13561 acgcttcgct gcttcagcat ctacccaaat attgaattct gctgtaggcg tccaatttcc
    13621 aaatgtacca ccacccatca aagtaataga ttcaatatgc tcagcgattc ttggctcacg
    13681 aatcaatgcc gttgctacat tcgtaagagg acctgtcgct acaattgtta caggtgtatc
    13741 actcgtcatc actttgttta taatcacatc tgatgctggc attgcaactg cttgacgtga
    13801 tggtgtcgac ggtagtttcg gaccatctaa tccagattcc ccatgtattt cagaagcaaa
    13861 ggcagctggt ttaattaacg gcctatccgc acctttcgct actgctatat cttggcgtcc
    13921 cataatatcc aatacgttca aggcgtttgt cgtattcttg tcaactgatt gattacctgc
    13981 gactgttgtt acagctaata tctctagtgg actgtcaatt gcccccgcta aaattaatgc
    14041 tattgcatca tcgtgtcctg gatcacaatc cataataatc tttcttttca tttatatatc
    14101 cacctttctt aagttgttat cgatagctta tgtatattta tttatgtggt gaatcatgtt
    14161 tattttgaaa aatagtttta actttctcat atttttggat acaaacacta tttatctatt
    14221 ttatggctta taaatttatc cgatatgcct tatcaaccta cctcgctaaa aataggatgt
    14281 ctacatatct ataccgactt ttgtcaactc attttcacaa caatataaac agcaatttat
    14341 atgattgtta catgattcaa acaattttta tgaaaaatat tttcatacac agaatatata
    14401 ttgatattaa atttctcaaa agctatattg agaataatta ggagggatgt tgatgaaatc
    14461 tttatttgaa aaagcacagc agttcggcaa gtcctttatg ttacctatcg caatcttacc
    14521 agctgcaggt ctattgttgg gtatcggtgg tgcattaagt aatccaaaca ccgttaaagc
    14581 ataccctatt ttagatatta ccttattaca aaatattttt acattaatgt cagctgcagg
    14641 tagtattgtt ttccaaaatt taccggtcat ctttgcaatt ggtgtcgcaa tcggattatc
    14701 tagaagcgat aaaggtactg caggtttagc tgcgctgctc ggtttcttaa ttatgaacgc
    14761 aactatgaat ggcttattaa ctatcacggg cacattggca aaagatcagc ttgcacaaaa
    14821 tggacaaggc atggtgctcg gtatacaaac ggttgaaacc ggtgtttttg gcgggattat
    14881 cacaggtatt atgaccgcaa tacttcacaa caaatatcac aaagtggtat taccaccgta
    14941 tttaggtttc tttggtggct ctagatttgt ccctattgtc acagcatttg ccgcaatctt
    15001 tttaggtgta ttgatgtttt tcatttggcc aagcatacaa gccggcattt atcatgttgg
    15061 tggatttgta acgaaaacag gtgccatcgg tacttttgtt tatggcttca tcttaagatt
    15121 gttaggtcca ctcggtttac accatatttt ttacttaccg ttttggcaga cggcacttgg
    15181 tggtacttta gaagtcaaag ggcacttagt tcaaggtacg cagaacatct tctttgctca
    15241 acttggtgat ccagatgtga cgaagtatta ttcaggtgtg tcacgcttta tgtcaggccg
    15301 ttttattacg atgatgttcg gcttatgtgg tgccgcactt gcaatttatc acacagctaa
    15361 acctgaacat aaaaaagttg tcggcggttt aatgttatcc gctgcactca cttcattttt
    15421 aacaggtatt accgaacctt tagagtttag tttcttgttt gtcgcaccta ttctttatgt
    15481 aatccatgcc ttctttgatg gattagcatt tatgatggca gacattttca acattacaat
    15541 tggtcaaacc ttcagtggag gctttatcga tttcttactc tttggtgtgc tacaaggtaa
    15601 tagtaaaaca aactacctat acgtcatacc tattggaatt gtgtggttct gtttgtatta
    15661 catcgttttc agattcttaa ttacgaaatt taatttcaaa acacctggtc gagaagataa
    15721 agctgcagca caacaagttg aggctactga aagagcacaa actattgttg ctggtttggg
    15781 aggcaaagat aacattgaaa tcgttgactg ttgtgcaacg agactacgcg tcacacttca
    15841 tcaaaatgac aaagtcgata aagtattact cgaaagtact ggtgccaaag gtgtaatcca
    15901 gcaaggcact ggtgtgcaag taatttatgg gcctcacgtt acagttatca aaaatgaaat
    15961 tgaagaattg ctcggggatt aagactaacc gaaatatcaa cagaactaat ggcaacgatg
    16021 tacgaagtaa gaagtgacat cgttgctttt atttttaatg ttacatttga agcattaagt
    16081 tcatcatgca ctgtagtgag cccgcaaatc gcctctgcta gacaatcatc ttaatgctat
    16141 gattaaagct taagtgccag atttgaattt aatttcaaca acgactttca ctacattaaa
    16201 aatagggcta ctcgacacat atagttgtat caaatagccc tttatacaat tttttgggta
    16261 aggttttaca atttttggaa tggtatagat tttataaaaa gttatttaag ttcttctgct
    16321 tcagccataa tatcttttaa tgttttagct gaatgtgcga acttgctttg ttcttcgtcg
    16381 tttaatggga tttctacgac gttgcgaata ccgtttctat tgatgactgc tggaacacca
    16441 atataaacat cttcttcatc atattcgcct tctaataatg ctgatacagt caatacggca
    16501 tcttcatttc tgaaaatcgc ttcagtaatt ctagctaatc ccattgcaac accataataa
    16561 gtggcacctt tagcttgaat aatgtcatat gctgcatcac gtgtttgaac aaaaatttgt
    16621 tcaatttgcg ctttgccctc aggacgttgt tcaagtaatg tcttcaaagg ttgacccgca
    16681 atattagcgt gtgaccatac tggtaattca gtgtcaccat gttcaccaat aatttgagca
    16741 tcgacgctac gtggcgcaac atcgaacgct tcgcttaaca ataatctaaa gcgtgcagag
    16801 tctaaaattg taccagaacc tataacacgt tctttaggta aaccagagaa tttccatgtt
    16861 gcatacgcta aaatatcaac aggatttgta gctaccaaga aaataccatc aaattttgat
    16921 gccattactt caccaacaat tgatttgaat attttcaagt ttttagatac taaatctaaa
    16981 cgtgtttctc caggtttttg tgcagcacca gcacagatga caactagatc cgcatcatga
    17041 caatcactgt attcgccagc tttcacacga actgttgttg gagaatatgg tgtggcatgt
    17101 tttaaatcca taacatctcc tcgaactttt tcagtgtcta aatcaatgat gactaattca
    17161 tcaacaatgc tttggttcac taatgaaaat gcgtagcttg aacctactgc accattacct
    17221 attaatacaa ctttgttccc tttaaatttg ttcattacaa aaactccctt atgattaatt
    17281 cactaacata cgtgtagttt caaatatgtt agtttaatgc tgcttattga cgatacaaaa
    17341 acaaataaac atctctttta ttttcaacgc ataacttaaa aggtcatgtg tcatccgctt
    17401 ttaagtttgt gatttatttc acatataaaa tgtaacatac attaagtact aggtcaatat
    17461 taaattgtga tttatttcac attttatttt aatttttaca cctttttaat ttgtatacga
    17521 ttacatctta gatgtcttta gtcttcgtac ttcgccagtg attatttaca ctttcacatt
    17581 tttattatca tgtttacttt tttctaagaa aacaacaatg ttttttgaat tagtcaaata
    17641 aatgcgcgca atcgtcggtg tgcaaacaga caattgtaca caatgcttat tgataagtat
    17701 ttaaaaaatt aaaaatgtca tacaattatc aaatttgcca ttttatttat attttctcaa
    17761 actaattaat tgaatatcga aatttttagt agaataatca aaatagacag attaaaggag
    17821 gagtatcatg cttacagaac aagagaaaga cattatcaaa caaacagtgc ctttacttaa
    17881 agagaaaggg acagaaatta cgtcaatctt ttatccaaaa atgtttaaag cgcatcctga
    17941 acttttaaac atgtttaatc aaacgaacca aaaacgaggc atgcaatctt cagcattagc
    18001 acaagctgta atggccgcag cggttaatat cgataactta agtgttatta aaccagtcat
    18061 tatgccagtc gcatataaac actgcgcact acaagtttat gctgaacatt atccaattgt
    18121 gggggaaaat ttattaaaag ccattcaaga cgtgacagga ttagaagaaa atgaccctgt
    18181 cattcaagct tgggcaaaag catatggcgt tattgcggat gtgttcatcc aaattgaaaa
    18241 agaaatttat gatcaaatga tgtggatagg ttttaaacca ttcaaaatta ccaatattaa
    18301 acaagaatct gaagacatta aatcatttac agttgaaact gaagaatatg actttagtga
    18361 atttacacca ggccaataca tcacagttga tgtttctagt gaaaaacttc catatagagc
    18421 taaacgtcac tattctatcg tatcaggtga aaaaaaccat ttaacttttg gcgttaaacg
    18481 tgatgtcaca acagaacatg aaggcgaagt ttcaacaatt ttacacgatg agattaaaga
    18541 aggcgatatg attaatttag ctgcgcctgt aggtggattc gtattagaga atacgactga
    18601 accacaactt ttcttaggtt caggtattgg tgttacacct ttagtagcta tgtatgaagc
    18661 tgcctctgcc aaaggtttag atacacagat ggttcaagtt gctgaaaatg aacaacattt
    18721 acctttcaaa gacaacttca acagtatcgc aagccatcat gacaacgcta aattatatac
    18781 acacttaaaa gataaacaag gctatattgg cgctgaagaa ttacaagcat ttttagcaaa
    18841 taaacctgaa atttatatct gtggtggtac aaaattctta caatctatga tcgaagcact
    18901 taaatcttta aattacgata tggatcgcgt acactacgaa acatttattc caagactaag
    18961 cgttgcagta taactgagaa aagaggttta gtgctcatat gaaacttaat caacgttacg
    19021 taaaagtatt tgcattatat ttcgtaagta ttgttactgc aaatattatt gttaaaaata
    19081 ataatttaat taaaacattg atacaaacca tagccgggta cacggtcttt gcagttggtt
    19141 taaagtattt aactaaacgt aaaaataaat aacatatata caaaaacagc tatgacttta
    19201 ctttgtcatg gcagtttttt aattgggagt gggacagtaa tgagattttc gcaaaattta
    19261 tttcgtcgtc ccaccacaac atgcattgat gtatgctcac tgaatttcat aagaaaggag
    19321 ttcacaagat gaccgtagat attggacgga tttatgacaa taaagataat accgacgcta
    19381 ttcgtatcct agtcgataga gtctggccga gaggtatttc gaaaagaact gctaacctag
    19441 attattggtt aaaagacatt gccccttcta ctgagttgcg acaatggttc caacatgatc
    19501 ctaaactttt tggagctttt aaagaaaaat atgaaaaaga attacgtgat caggatgcac
    19561 aaaaagatgc ttttgaaaaa ttaaaggata ttgtaaatca gcataatcat gttctattgt
    19621 tatatgcagc aaaagatact aaacataacc aagctgtagt actacagcag ttgctcaata
    19681 cttaggtata tatgtttatc gtgaatcact ataaatatcg ctaacttcat ttctaatatt
    19741 tatttttaaa aagcataatt actacaatta attgaacttt aatgattact aaacttgaac
    19801 aata
//
LOCUS       DAMZKC010000038        19710 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.038, whole genome shotgun sequence.
ACCESSION   DAMZKC010000038 DAMZKC010000000
VERSION     DAMZKC010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 19710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19710
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.038"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(67..477)
                     /locus_tag="POB55_000992"
     CDS             complement(67..477)
                     /locus_tag="POB55_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDI6499965.1"
                     /translation="MNTNDAIKILKENGLKYTDKRKDMLDIFVEEDKYINAKYIQQVM
                     DENYPGISFDTIYRNLHLFKDLGIIENTELDGEMKFRIACTNHHHHHFICEKCGDTKV
                     IDYCPIDQIKLSLPGVNIHKHKLEVYGVCESCQD"
     gene            complement(464..1327)
                     /locus_tag="POB55_000993"
     CDS             complement(464..1327)
                     /locus_tag="POB55_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDI6499966.1"
                     /translation="MIDALLNFDFMRYSLISGILIGFIAPLIGAFIVVRRLSLIADAL
                     SHVTLGGISFGMFLLTIMPTLVFINPMWFGILFAIVGALLIEKLRTSYTAYQEIAIPI
                     IMSAGIALSAIFISLADGFNQEIVGLLFGSISAVNISDLTTIIVIAIIVVLFITLFYK
                     ELFILSFDEEYSKVIGIPKWIQFLFIVIVAMVISASMRVVGILLVSALITLPIAISMR
                     ITKSFKQLILLSVFLGELSVILGLVLAFYMDISPGGVIVVLLVILLMITMAYQKMRMK
                     FKKGANINEYK"
     gene            complement(1369..2154)
                     /locus_tag="POB55_000994"
     CDS             complement(1369..2154)
                     /locus_tag="POB55_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDI6499967.1"
                     /translation="MRGENTMTPVFELKNVNYYYDHKKVLENINIKINKGEFLAIVGP
                     NGAGKSTLLKLILGLLPLQSGEIFVEGIDFKNKKTSIKLSYVSQKANAFNSGFPASVK
                     EVVLSGLTKTKRLFQTFNSKDNEKVIKVLERLNISDLIHKNIAELSGGQQQRVMIARA
                     LISEPAVLVLDEPTNGIDAKHVSEFYNTLDQLKQEGITIILVTHDIGVVADTATEVAC
                     LNKHLHFHGTTDEFKSLDEVEISKIYGHPVRFVDHQHNRECCN"
     gene            complement(2280..3170)
                     /locus_tag="POB55_000995"
     CDS             complement(2280..3170)
                     /locus_tag="POB55_000995"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HDI6499968.1"
                     /translation="MLLGSHVSMSGKKMLEGSAIEAYEYGETTFMIYTGAPQNTRRKS
                     IEDLNITKGHEVMEKYGLSNIVVHAPYIINIANTTKPETFNLGVDFLQQEIERTQAIG
                     AKDIVLHPGAHVGAGVDAGINKIIEGLNEVLTNDNNVRIALETMAGKGTEIGRSFEEL
                     ARIIDGVHNNERLSVCFDTCHTHDAGYNVKEDFDGVLNEFDKIIGVDRIKVVHVNDSK
                     NDRGAQKDRHENIGFGYIGFDALNYIVHHDSFKDIPKILETPYVGEDKKNKKPPYKLE
                     IEMLKQQQFDPELKNKVMQQ"
     gene            complement(3180..4526)
                     /locus_tag="POB55_000996"
     CDS             complement(3180..4526)
                     /locus_tag="POB55_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDI6499969.1"
                     /translation="MAKHPFEQFNLESSLIDAVKDLNFEKPTEIQNRIIPRILKRTNL
                     IGQSQTGTGKSHAFLLPLMQLIDSEIKEPQAIVVAPTRELAQQLYDAANHLSQFKAGV
                     SVKVFIGGTDIEKDRQRCNAQPQLIIGTPTRINDLAKTGHLHVHLASYLVIDEADLMI
                     DLGLIEDVDYIAARLEDNANIAVFSATIPQQLQPFLNKYLSHPEYVAVDSKKQNKKNI
                     EFFLIPTKGAAKVEKTLNLIDILNPYLCIIFCNSRDNANDLARSLNEAGIKVGMIHGG
                     LTPRERKQQMKRIRNLEFQYVIASDLASRGIDIEGVSHVINFDVPNDIDFFTHRVGRT
                     GRGNYKGVAITLYSPDEEHNISLIEDRGFVFNTVDIKDGELKEVKAHNQRQARMRKDD
                     HLTNQVKNKVRSKIKNKVKPGYKKKFKQEVEKMKRQERKQFSKQQNRQKRKQNKKG"
     gene            complement(4640..5740)
                     /locus_tag="POB55_000997"
     CDS             complement(4640..5740)
                     /locus_tag="POB55_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HDI6499970.1"
                     /translation="MKIADLMTLLDHHVPFSTAESWDNVGLLIGDEDVEVTGVLTALD
                     CTLEVVNEAIEKGYNTIISHHPLIFKGVTSLKANGYGLIIRKLIQHDINLIAMHTNLD
                     VNPYGVNMMLAKAMGLKNISIINNQQDVYYKVQTYIPKDNVGPFKDKLSENGLAQEGN
                     YEYCFFESEGRGQFKPVGEANPTIGQIDKIEYVDEVKIEFMIDVYQKSRAEQLIKQYH
                     PYETPVFDFIEIKQTSLYGLGVMAEVDNQMTLEDFAADIKSKLNIPSVRFVGESNQKI
                     KRIAIIGGSGIGYEYQAVQQGADVFVTGDIKHHDALDAKIHGVNLIDINHYSEYVMKE
                     GLKTLLMNRFNTEKINIDVEASTINTDPFQYI"
     gene            complement(5743..6420)
                     /locus_tag="POB55_000998"
     CDS             complement(5743..6420)
                     /locus_tag="POB55_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HDI6499971.1"
                     /translation="MISLNNRLTTVSRFLKQGTIADIGSDHAYLPIYAIQNHLCECGI
                     AGEVIQGPFQAAVKNVAANQLVDRIDVRLGDGLSVIQPEDVIDNITICGMGGPLIAKI
                     LKDGQDKLSQHPRLILQSNIQTENLRQTLQQLNYEIIDEIIMEEKGHIYEIVVAEYST
                     QLIELSSDGLKFGPKLLNNKNEYFIKKWQRELEALYHIKSKLNTEQHHQRLAQINDEI
                     AVIERVL"
     gene            complement(6551..7657)
                     /gene="rpoD"
                     /locus_tag="POB55_000999"
     CDS             complement(6551..7657)
                     /gene="rpoD"
                     /locus_tag="POB55_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HDI6499972.1"
                     /translation="MSDNTVKIKKQTIDPTLTLEDVKKQLIEKGKKEGHLSHEEIAEK
                     LQNFDIDSDQMDDFFDQLNDNDISLVNEKDSSDTDEKLNPSDLSAPPGVKINDPVRMY
                     LKEIGRVNLLSAQEEIELAKRIEQGDEVAKSRLAEANLRLVVSIAKRYVGRGMLFLDL
                     IQEGNMGLIKAVEKFDFNKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LIRVQRQLLQDLGRDPAPEEIGEEMDLPAEKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDQEAQSPSDHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVGK
                     VFGVTRERIRQIEAKALRKLRHPSRSKRLKDFMD"
     gene            complement(7881..9698)
                     /locus_tag="POB55_001000"
     CDS             complement(7881..9698)
                     /locus_tag="POB55_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDI6499973.1"
                     /translation="MRIDQSIINEIKDKTDILDLVSEYVKLEKRGRNYIGLCPFHDEK
                     TPSFTVSEDKQICHCFGCKKGGNVFQFTQEIKDISFVEAVKELGDRVNVAVDIEATQF
                     NSNIQIASDDLQMIEMHELIQEFYYYALTKTVEGEQALTYLQERGFTDALIKERGIGF
                     APDSSHFCHDFLQKKGYDIELAYEAGLLSRNEENFSYYDRFRNRIMFPLKNAQGRIVG
                     YSGRTYTGQEPKYLNSPETPIFQKRKLLYNLDKARKSIRKLDEIVLLEGFMDVIKSDT
                     AGLKNVVATMGTQLSDEHITFIRKLTLNITLMFDGDFAGSEATLKTGQHLLQQGLNVF
                     VIQLPSGMDPDEYIGKYGNDAFTAFVKNDKKSFAHYKVSILKDEIAHNDLSYERYLKE
                     LSHDISLMKSSILQQKALNDVAPFFNVSPEQLANEIQFNQAPANYYPDDEYGGYDEYG
                     GYIEPEPIGMAQFDNLSRQEKAERAFLKHLMRDKDTFLNYYESVDKDNFTNQHFKYVF
                     EVLHDFYAENDQYNISDAVQYVNSNELRETLISLEQYNLNDEPYENEIDDYVNVINEK
                     GQETIESLNHKLREATRIGDVELQKYYLQQIVAKNKERM"
     gene            complement(9759..10577)
                     /locus_tag="POB55_001001"
     CDS             complement(9759..10577)
                     /locus_tag="POB55_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HDI6499974.1"
                     /translation="MEKIKIIVASDSIGETAELVARAGISQFNPKQCKNELLRYPYIE
                     SFEDVDEVIQVAKDTNAIIVYTLIKPEMKQYMSEKVAEFQLKSVDIMGPLMDLLSASV
                     EEKPYNEPGIVHRLDDAYFKKIDAIEFAVKYDDGKDPKGLPKADIVLLGISRTSKTPL
                     SQYLAHKSYKVMNVPIVPEVTPPDGLYDIDPKKCIALKISEEKLNRIRKERLKQLGLG
                     DTARYATEARIQEELNYFEEIVSEIGCPVIDVSQKAIEETANDIIHYIEQNKSK"
     gene            complement(10588..11211)
                     /locus_tag="POB55_001002"
     CDS             complement(10588..11211)
                     /locus_tag="POB55_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001797828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI6499975.1"
                     /translation="MELSQRQERIIEIVKTKGPITGEQIADKLNLTRATLRPDLAILT
                     MSGFLEARPRVGYYYSGKSKGKFFNEKLRQFEVKDYMSQPVVLRENTTVYDAICTIFL
                     EDVSTLFIINEDNDFVGVCSRKDLLRASMIGADIHTVPISVNMTRMPNVTYLEESELV
                     IYAADRMIEKEIDSIPIVRKKDNQKYEVIGRISKTTIAKLLVALYKE"
     gene            11546..12937
                     /locus_tag="POB55_001003"
     CDS             11546..12937
                     /locus_tag="POB55_001003"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HDI6499976.1"
                     /translation="MAKDMDTIVSLAKHRGFVFPGSDIYGGLSNTWDYGPLGVELKNN
                     VKKAWWQKFITQSPFNVGIDAAILMNPKVWEASGHLNNFNDPMIDNKDSKIRYRADKL
                     IEDYMQDVKGDENFIADGLSFEQMKKIIDDEGIVCPVSKTANWTEIRQFNLMFKTFQG
                     VTEDSTNEIFLRPETAQGIFVNYKNVQRSMRKKLPFGIGQIGKSFRNEITPGNFIFRT
                     REFEQMELEFFCKPGEEIEWQNYWKTFASDWLTSLNMSSENMRLRDHDEDELSHYSNA
                     TTDIEYKFPFGWGELWGIASRTDFDLRKHAEHSGEDFRYHDPETNEKYIPYCIEPSLG
                     ADRVTLAFLCDAYDEEGVEGSKDARTVLHFHPALAPYKAAILPLSKKLSGEAIKIFEQ
                     LSSKFSIDFDESQSIGKRYRRQDEIGTPYCVTFDFDSLEDNQVTVRDRDSMEQVRMPI
                     SELEAFLTEKTKF"
     gene            complement(13088..13834)
                     /gene="recO"
                     /locus_tag="POB55_001004"
     CDS             complement(13088..13834)
                     /gene="recO"
                     /locus_tag="POB55_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HDI6499977.1"
                     /translation="MRQKGIIIKAVDYGESDKIITILNEHGAKVPLMARRAKKVKTGL
                     QAQTQLFVYGLFIYNQWRGMGTLNSVDVISQHYKLQMDLFVSSYASLAAETIERSMDE
                     GDIAPYNYQLLQFVLEKIESGTSAQLMSVVVMLKCMKRFGFTASFNRCAVSGNDTQAD
                     LIGYSFKFDGAISRQEASKDVHAVILSNKTLYLLDVLQKLPIDKMNSLNIHQEIIDEM
                     SDIILMLYREYAGMFFKSQKLINQLKRLEQ"
     gene            complement(13862..14761)
                     /gene="era"
                     /locus_tag="POB55_001005"
     CDS             complement(13862..14761)
                     /gene="era"
                     /locus_tag="POB55_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HDI6499978.1"
                     /translation="MTEHKSGFVSIIGRPNVGKSTFVNRVIGHKIAIMSDKAQTTRNK
                     IQGVMTRDDAQIIFIDTPGIHKPKHKLGDYMMKVAKNTLSEIDAIMFMVNANEEIGRG
                     DEYIIEMLKNVKTPVFLVLNKIDLVHPDELMPKIEEYQSYMDFTEIVPISALEGLNVD
                     HFIDVLKTYLPEGPKYYPDDQISDHPEQFVVGEIIREKILHLTSEEIPHAIGVNVDRM
                     VKESEDRVHIEATIYVERDSQKGIVIGKGGKKLKEVGKRARRDIEMLLGSKVYLELWV
                     KVQRDWRNKVNFIRQIGYVEDQD"
     gene            complement(14762..15166)
                     /gene="cdd"
                     /locus_tag="POB55_001006"
     CDS             complement(14762..15166)
                     /gene="cdd"
                     /locus_tag="POB55_001006"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDI6499979.1"
                     /translation="MSYQPHYFQEVRKAQQESYSPYSQFKVGAYLKTKDGRTFYGTNV
                     ENASYPLSICAERASLVSAISQGYRPGDFESITVTVDADKPSSPCGACRQVLKELCDD
                     DMPVYMTNHKGDMVMMTVAELLPFGFSGKDLE"
     gene            complement(15177..15521)
                     /locus_tag="POB55_001007"
     CDS             complement(15177..15521)
                     /locus_tag="POB55_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDI6499980.1"
                     /translation="MKRFKYALDGLKILIQKDYKFLLHVFAMIVAIVFGLVLNINRIE
                     WIFILIAIALVLTVEALNTAIEYVVDLVTVEYHDLAKYAKDIAAFSVLIVSILAFIIG
                     LIVFLPHFIALF"
     gene            complement(15524..15991)
                     /gene="ybeY"
                     /locus_tag="POB55_001008"
     CDS             complement(15524..15991)
                     /gene="ybeY"
                     /locus_tag="POB55_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HDI6499981.1"
                     /translation="MFTIDFSDHTGLVKDAWYKQIEDLLEFAKKEEHIEDDAELSVTF
                     VDKQEIQEINRTYRDKDKVTDVISFALEEDEPEIDFSGLDIPRVLGDIIICTDVAQEQ
                     ANNYGHSFERELGFLALHGFLHLLGYDHMTEADEKEMFGRQDTILNAYGLTRG"
     gene            complement(15992..16939)
                     /locus_tag="POB55_001009"
     CDS             complement(15992..16939)
                     /locus_tag="POB55_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HDI6499982.1"
                     /translation="MPGIIQIDDMNQSQALIGNNDEHLKAIEESFDVVIHARGQEVAV
                     KGTKIENVEKAESVLINLLKVIDLGNNITIKDVEAAIKMAHNNTIQHLLDLYDEEITK
                     DAFGKTIRAKTMGQRIYVNAMKNNDLVFGIGPAGTGKTFLAVVYAAKQLRKGAVKRIV
                     LTRPAVEAGESLGFLPGDLKEKVDPYLRPLYDGLYTVLGREQTERFIERGIIEIAPLA
                     YMRGRTLEDAFVILDEAQNMTHAQMKMFLTRLGFGSKMVVTGDQTQIDLPKGVKSGLK
                     EAVSRLHNVKGISILKLDQSDVVRHPLVSKIIEHYEGEN"
     gene            complement(17243..17941)
                     /locus_tag="POB55_001010"
     CDS             complement(17243..17941)
                     /locus_tag="POB55_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron transporter"
                     /protein_id="HDI6499983.1"
                     /translation="MSVGILIFVISVIISIITTMRENSHKDRQNQKPPQKTSTDNEPK
                     KGGFFEEIERTFKEISEELNEEEKKSSKRKYDDTLPPLFDELPKEEPKSKPVVEPMAP
                     KKQPETKPMTEKPITVPKAEPVEQKHRPSRQDNSDEIRRQLEKSLRDDIKTIRTDIDR
                     EKEKQIAKMEKRARDIIEDKYLSERTKRLKLKQLLNSQNVEKDLTKSAFQFDKDEVIN
                     GMIWSEILAKPKQL"
     gene            complement(17958..18947)
                     /gene="floA"
                     /locus_tag="POB55_001011"
     CDS             complement(17958..18947)
                     /gene="floA"
                     /locus_tag="POB55_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500186.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HDI6499984.1"
                     /translation="MFSLSFIVIAVIIVVALLILFSFVPIGLWISALAAGVHVGIGTL
                     VGMRLRRVSPRKVIAPLIKAHKAGLALTTNQLESHYLAGGNVDRVVDANIAAQRADID
                     LPFERAAAIDLAGRDVLEAVQMSVNPKVIETPFIAGVAMNGIEVKAKARITVRANIAR
                     LVGGAGEETIIARVGEGIVSTIGSSKHHTEVLENPDNISKTVLSKGLDSGTAFEILSI
                     DIADVDISKNIGADLQTEQALADKNIAQAKAEERRAMAVATEQEMKARVQEMHAKVVE
                     AESEVPLAMAEALRSGNISVKDYYNLKNIEADTGMRNAINKRTDQSDDESPEH"
     gene            complement(18965..19648)
                     /locus_tag="POB55_001012"
     CDS             complement(18965..19648)
                     /locus_tag="POB55_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HDI6499985.1"
                     /translation="MTTILESTAGDTWVEQVSNIIVQPIFTLILTCLTFLGFVYQLYS
                     KKINAAGIIATLSLLILFLGFLIQGNVNMHSILIFSIGVILVVIELFVVGAVIGIIGM
                     ILITISITTLGDNLLFMLANVIVALILTIVEWVILVKIFNRKIPFLDKVILKDSTNSE
                     SGYNSHDNRSHLVGKTAQTVTDLRPAGIIFCENERIDAVSDGNFILRNKTVKILEVEG
                     TRVVVREVD"
BASE COUNT         6177 a         3686 c         2667 g         7180 t
ORIGIN      
        1 tataatttaa aaaatgagat ttatgcatac atctgaccaa atgtgcataa atctcatttc
       61 tttatattaa tcttggcaag actcacatac accataaact tcaagtttgt gtttgtgaat
      121 attaacacca ggtagtgata actttatctg atctattgga caataatcta ttacctttgt
      181 atctccacac ttttcacaga taaaatgatg atgatgatgg tttgtacaag cgattctaaa
      241 cttcatttca ccatcaagtt ctgtattttc aataatccct aaatctttaa ataagtgcag
      301 gtttctatat attgtgtcga atgaaattcc aggataattt tcatccataa cttgctgtat
      361 atactttgcg tttatatact tatcttcttc gacaaaaata tctaacatat ctttacgttt
      421 atctgtatat tttaaaccgt tctcttttaa aattttaata gcatcatttg tattcattga
      481 tattagctcc ctttttaaac ttcattcgca ttttctgata agccattgta atcataagta
      541 aaataacaag tagaactaca attacaccac ccggagaaat gtccatatag aaagctagga
      601 ctaagcctaa tattactgat aattcaccta aaaatacact tagtaatatc aattgcttaa
      661 aactttttgt tattcgcata cttattgcaa ttggtaacgt gattaacgca cttactaaca
      721 gtatccctac aacacgcatt gaggcagaaa taaccatcgc tacaataaca ataaataaaa
      781 attgaatcca tttaggaatg ccaatgactt tactatattc ctcatcaaat gacaatataa
      841 ataattcttt ataaaacaat gtaataaaca gaacaactat gatggcaatg acaataatcg
      901 ttgttaaatc acttatattc actgcgctta ttgagccaaa tagcaatcca acaatttctt
      961 gattgaaccc atcagctaat gaaatgaaga ttgcactcaa ggcgatacca gcactcatta
     1021 taattggaat agcaatttct tggtaagcag tgtatgacgt tcttaatttt tcaattagaa
     1081 gcgcacctac tattgcgaat aagattccaa accacattgg attaataaat actagtgttg
     1141 gcataatagt aagtaaaaac ataccgaaag atataccacc taaagttaca tgacttagag
     1201 catcagctat aagtgatagt cgtctaacaa cgataaaagc accgattaga ggcgcaataa
     1261 aacctatcaa gataccacta attaaagagt acctcataaa atcaaaattc aataatgcat
     1321 ctatcaattg tgacacgcct ttccatttta aataaactca aatctttatt aattacaaca
     1381 ttctcgatta tgctgatgat cgacaaaacg tacaggatgt ccataaattt ttgaaatttc
     1441 aacttcatca agtgatttaa actcatcagt tgtaccatgg aaatgcaaat gcttatttaa
     1501 acatgctact tcagtagcag tatctgctac aacaccgata tcatgagtaa ctaagataat
     1561 ggtgatacct tcttgtttta attgatctaa agtattataa aattcactta catgttttgc
     1621 atcaatacca ttcgttggtt catcaagtac taatactgca ggttctgaaa tcaatgctcg
     1681 agcaatcatt acacgttgtt gttgaccacc tgataattct gctatatttt tatgaattaa
     1741 atcacttata ttcagtcttt ctagtacttt aatcactttt tcattatctt tgctattaaa
     1801 tgtttggaaa agacgttttg tctttgttaa tccgcttaaa acaacttctt taacacttgc
     1861 tgggaaacct gaattaaagg catttgcttt ttgtgataca tagcttaatt taattgatgt
     1921 tttcttattt ttaaaatcaa taccttcaac aaaaatctca ccactttgta aaggtaataa
     1981 ccctagaatc aacttcaata atgttgattt accagcacca tttggtccaa caattgctaa
     2041 aaattcacct ttatttattt taatgtttat attttctaac acttttttat gatcatagta
     2101 gtaattgaca tttttcaatt caaagactgg tgtcatcgta ttctcacctc gcattcaact
     2161 atacaactcc tagtaacata tgtaaacagt aatgtttacg actcaaaatt agacaaaata
     2221 aagagatatg cccccttcaa gttttattta tcgcatttct tgaagagagc attatcattt
     2281 tattgttgca taaccttatt ttttaattct gggtcaaatt gctgttgttt taacatttca
     2341 atttcaagtt tatatggcgg ttttttattt ttcttatctt caccaacata aggtgtttct
     2401 aagattttcg gaatatcttt aaaactatca tgatgcacaa tgtaatttaa tgcatcaaaa
     2461 ccaatgtaac cgaagccaat attttcatgt cggtcttttt gagcgccacg gtcattttta
     2521 gaatcattga catgaacaac tttgattctg tcgactccaa taattttatc aaattcattt
     2581 aatacgccat caaagtcctc tttcacatta tatccagcat catgcgtatg acatgtatca
     2641 aaacatactg ataaacgttc gttattatga actccatcaa taatacgtgc taactcttca
     2701 aacgagcgac caatctctgt acctttacct gccatcgttt caagcgcaat acgtacatta
     2761 ttgtcattcg ttaaaacttc atttaatcct tcaataatct tattaattcc ggcatcaaca
     2821 ccagctccaa catgcgcacc tggatgtaat acaatatctt tagcgcctat agcttgcgtt
     2881 ctttcaattt cttgttgcaa gaaatctaca ccaagattaa acgtttctgg tttggttgta
     2941 tttgcaatat taatgatgta tggtgcatga acaacaatat tagataagcc atatttttcc
     3001 atcacttcat gacctttagt tatatttaaa tcttcaatac ttttacggcg cgtgttttga
     3061 ggtgcaccag tataaatcat aaatgttgtt tcaccatatt catacgcttc tatagcagaa
     3121 ccttctaaca tctttttacc actcattgaa acatgtgatc ctaataacat aaaacacacc
     3181 taaccttttt tgttttgctt acgtttttgt ctattttgct gcttactaaa ttgtttacgc
     3241 tcttgacgtt tcattttttc aacttcttgt ttaaatttct tcttataacc tggtttaact
     3301 ttgtttttaa ttttacttcg aactttgttc ttcacttgat tagttaaatg gtcatctttg
     3361 cgcattcttg cttgacgctg attgtgcgct ttaacttctt ttaactcacc atctttaata
     3421 tcaacagtat tgaatacaaa accgcgatct tctattaatg aaatattgtg ttcttcatca
     3481 ggactataaa gcgtaattgc tacaccttta taattcccac gaccagttcg tccaactcta
     3541 tgcgtaaaga agtcaatatc atttggcaca tcaaaattga tgacatgact aacaccttca
     3601 atatcaatac cacgagatgc taaatcgctg gcaataacgt attggaattc taaattacgt
     3661 atacgtttca tttgttgttt acgttcacgt ggcgttaaac caccatgaat cataccaact
     3721 ttaataccag cttcatttag tgaacgtgct aaatcatttg cattatctct actattacag
     3781 aaaataatac ataagtatgg atttagtata tcaattaaat ttaaagtttt ttcaacttta
     3841 gctgcacctt tagtaggtat taaaaagaat tcgatgttct ttttattttg ttttttactg
     3901 tcgacagcta catattctgg atgacttaaa tatttattta aaaatggttg taactgttgt
     3961 ggaattgtag cactaaacac cgcaatattt gcattatctt ccaatcttgc agcaatgtaa
     4021 tctacatctt caattaatcc taagtcaatc ataagatccg cttcatcaat aactaaatat
     4081 gatgctaagt gcacatgtaa atgtcccgtt ttagctaagt cattaattct agtaggggtg
     4141 cctataatca attgtggttg tgcattacaa cgttgtctat ctttctctat atctgtacca
     4201 ccaataaaaa ctttaactga aacaccagct ttaaattggc ttaaatggtt cgctgcatcg
     4261 tatagttgtt gtgcaagttc tcttgttggt gcaactacga ttgcttgtgg ttcttttatt
     4321 tcactatcaa ttaactgcat taatggtaat aaaaatgcat gagatttccc tgtacccgtt
     4381 tgagattgac caattaaatt tgttctcttt agtattcttg gaataattcg attctgaatt
     4441 tcagttggtt tttcaaaatt aaggtctttc acagcgtcaa ttaaactaga ttctagatta
     4501 aattgttcga atggatgttt tgccatgatt tggcctcctt atatgttcat ctttactatt
     4561 ataaagtata aatgtgctat tttaaataaa aatattaaaa cctcgaaatt tcaaaattta
     4621 gaaatcccga gattttagtt taaatatatt gaaatggatc tgtattaatt gtagatgctt
     4681 caacatctat atttattttt tctgtattga accgattcat tagtaacgtt tttaaacctt
     4741 ctttcatcac atattcgctg taatgattaa tatcaattaa attcacacca tgaattttag
     4801 catctaaggc atcatgatgt ttaatatcac ctgtaacaaa gacatctgcg ccttgttgga
     4861 cagcttgata ttcatatcca atacctgaac caccaataat tgcaatacgt ttaattttct
     4921 gattagactc accaacaaaa cggacacttg ggatatttaa tttagattta atatcagctg
     4981 cgaaatcttc caatgtcatt tgattatcca cttctgccat aacgccaagt ccataaaggg
     5041 atgtttgttt tatctcaata aaatcaaata ccggtgtttc atatggatgg tattgtttaa
     5101 ttaattgctc agcccttgac ttttgatata catctatcat aaattcaatt ttaacttcat
     5161 ctacatattc aattttatca atttgtccta ttgttggatt agcttcacca actggtttga
     5221 attgccctct tccttcactt tcaaagaaac aatattcata attaccttct tgcgctaatc
     5281 cattttcact aagcttatct ttaaatggtc caacattatc cttaggtata tatgtttgaa
     5341 ctttatagta tacatcttgt tgattattta ttattgaaat gttcttcaaa cccatcgcct
     5401 tcgccaacat catattgaca ccatacggat ttacatctaa atttgtatgc atcgctatta
     5461 aattaatgtc atgttgaatt agttttctaa tgatcaaacc ataaccatta gcttttaatg
     5521 atgttacgcc tttaaagatt agaggatgat gactaataat agtattataa cctttttcga
     5581 ttgcttcatt tactacttcc aacgtacagt ctaatgctgt taaaacacca gtaacttcaa
     5641 catcttcatc acctattaac aatcctacat tatcccaaga ttcagcagta ctaaatggca
     5701 catgatgatc taacaatgtc attaaatcag ctattttcat tactataaca ccctttcaat
     5761 tacagcaatt tcgtcattaa tttgagctaa acgttgatga tgttgttcag tattgagttt
     5821 cgatttaata tgataaagtg cttctaactc tctttgccat ttttttataa aatattcatt
     5881 tttgttgttc agcaattttg gtccgaattt caatccatca gatgatagct ctattaattg
     5941 tgtagaatat tctgctacaa caatttcata aatatggcct ttttcttcca ttattatttc
     6001 atcaattatt tcataattca attgttgtaa tgtttgtctt aaattttcag tttggatatt
     6061 actttgtaaa atcaaccttg gatgttgact taacttatct tgcccatctt ttaaaatttt
     6121 agcaataagt ggtccgccca taccacatat tgtgatatta tcgattacgt cctcaggttg
     6181 aataacactt aagccatccc ctaaacgtac atcaattcta tctactaatt ggtttgcagc
     6241 tacatttttt acagcagctt gaaaagggcc ttgaataact tctccagcaa taccgcattc
     6301 gcataaatgg ttttgaattg catagattgg caaataagca tgatctgagc caatatccgc
     6361 gattgtacct tgttttaaaa atcgacttac cgtcgttaat cggttattta acgaaatcat
     6421 tttattctcc tttatgaaaa aagaggttga acattattca atgtaccaac ctcactcaag
     6481 ttaaatctta atctaaaaat aaataagcgc ttattcatgt cttggtatca atcattaaaa
     6541 ttgatataaa ttaatccata aagtctttca aacgtttact acgacttgga tgtcttaatt
     6601 ttctaagtgc ttttgcttca atttgtcgaa tacgttcacg tgtaacaccg aaaactttac
     6661 caacttcttc aagtgttctt gttctgccgt catcaagacc aaatcttaat cgtaatacat
     6721 tttcttctct atcagttaat gtatcaagca catcttctaa ttgctctttt aataattcat
     6781 aagcagcatg atctgaagga ctttgtgctt cctgatcctc aataaagtct cctaaatgac
     6841 tatcatcttc ttcaccaatt ggcgtttcta atgaaacagg ttcttgcgca atttttaaaa
     6901 tttcacgaac tttttctgct ggtaaatcca tttcttcacc aatttcttct ggtgctggat
     6961 ctcgacctaa gtcctgtaat aattgacgtt gaacacgaat taatttatta attgtttcta
     7021 ccatatgcac agggatacga atcgtacgtg cttggtcagc aattgcacga gtgattgctt
     7081 gtctaatcca ccatgttgca tatgttgaaa acttaaatcc tttgttaaag tcaaattttt
     7141 caacagcttt aataagaccc atattacctt cttggattaa atcaaggaat aacataccac
     7201 gacctacgta tcttttagca atacttacaa ctaaacgtaa gttcgcttct gctagtcttg
     7261 attttgctac ttcatcacct tgttcaatac gtttggctaa ttcgatttct tcttgtgcac
     7321 ttaataagtt aacacgccca atttctttaa ggtacatacg aactgggtca tttattttaa
     7381 cacctggagg ggcactaaga tcacttggat tcagtttctc gtcagtatct gaactatctt
     7441 tttcattaac tagtgaaata tcattatcat ttaattgatc aaagaaatca tccatttgat
     7501 cagagtcgat atcaaaattc tgaagttttt cagcaatttc ttcatgactt aaatgaccct
     7561 cttttttacc cttttcaatt aattgcttct taacatcttc taatgttaat gtcggatcaa
     7621 ttgtttgttt tttaatttta actgtgttat cagacatgaa acggcctccc gattttaaat
     7681 atgaacattc gaaatttatt caatattgct attttaaact aaattcttaa ttaattcatc
     7741 catattttta attttatttt acaaattgga actaatccca atatttattt ttcaatagtg
     7801 ttgtttaaat gcggtaacta tcatttaccc ttatacattt tgacataatc attattcgta
     7861 ttattcttta aaatcacatg ctacatgcgt tctttattct tagcaacaat ttgctgtaaa
     7921 tagtattttt gtaattctac atcgccaatc cttgtagctt cccttaattt atgattcaat
     7981 gactcaattg tttcttgtcc tttttcatta ataacattga cataatcatc aatttcattt
     8041 tcatatggtt cgtcattcaa attatattgt tctaagctaa ttagtgtttc tctcaactca
     8101 tttgaattaa catactgcac agcatcactg atattatatt gatcattttc cgcataaaaa
     8161 tcatgtaaga cttcgaatac atatttaaaa tgctgatttg tgaagttatc cttatcaaca
     8221 ctttcataat aatttaaaaa tgtatcttta tctctcatta aatgttttaa aaatgctcgc
     8281 tccgcttttt cttgacggct caaattgtca aattgtgcca taccaattgg ctcaggttca
     8341 atataaccgc catactcatc ataaccgcca tactcatcat ctggataata attggctggt
     8401 gcttgattga attgtatttc gttagctaat tgctcaggac taacattgaa aaatggtgca
     8461 acatcattta aagccttttg ttgcaaaatc gatgatttca taagcgaaat atcatgactt
     8521 agttctttca aataacgttc atatgaaagg tcattatgtg caatttcatc ttttaatata
     8581 ctcactttat aatgtgcaaa tgactttttg tcatttttta caaaagcagt aaatgcgtcg
     8641 ttgccatact taccaatgta ttcatccgga tccatgcctg atggcaattg tataacaaat
     8701 acatttagcc cttgctgtaa taaatgttga cctgttttaa gtgttgcttc actacccgca
     8761 aaatccccat caaacattaa tgttatattt aatgttaact ttcgtataaa agtaatatgt
     8821 tcatctgaca actgtgtccc cattgttgca acaacgtttt tcaagccagc agtatcagat
     8881 tttataacat ccataaaacc ttctagtaat acgatttcat ctaattttct aattgattta
     8941 cgcgctttat ctaagttgta taacaacttt cttttttgaa agataggtgt ttcaggacta
     9001 tttaggtatt ttggttcttg accggtatat gttcgacctg aatatccaac aattcttcct
     9061 tgcgcatttt tcaaaggaaa cataatacga tttcgaaatc tatcgtaata actgaaattt
     9121 tcttcgttac gtgataataa tccggcttca tatgctaatt caatatcgta accctttttt
     9181 tgaagaaaat catgacaaaa atgtgagcta tcgggtgcaa agccaatgcc tcgctcttta
     9241 ataagcgcat ctgtaaaacc acgttcttgt aagtatgtta atgcttgttc gccttcgact
     9301 gtctttgtta aagcgtaata ataaaattct tgtattaact catgcatttc aatcatttgt
     9361 aaatcatcag aagcaatttg aatatttgag ttaaattgtg ttgcctcaat atctacagca
     9421 acattaactc tatcacctaa ttctttaacc gcttcaacaa atgatatgtc tttaatttct
     9481 tgagtaaatt gaaaaacatt gccacctttt ttacaaccaa aacaatgaca gatttgttta
     9541 tcttcagaaa ctgtaaatga aggtgtcttt tcatcatgaa aaggacacaa acctatataa
     9601 ttgcgtcctc tcttttctag ttttacatat tcacttacca agtctaaaat gtcggtttta
     9661 tcttttattt cattaatgat cgattgatct attcgcaaat taaatcacct attaagcatt
     9721 agttatacta ttatacctaa tttttgacaa aaatgaaatc atttcgattt attttgttca
     9781 atataatgga ttatatcgtt tgctgtttct tcgattgctt tttgagaaac atcaatgaca
     9841 ggacatccaa tttcacttac gatttcttca aagtaattca attcttcttg gattcgagct
     9901 tctgttgcat aacgagctgt gtcacctagt cccaattgtt ttaatcgctc ttttctaatg
     9961 cgatttaatt tttcttcact tattttaagt gcgatacatt tctttggatc aatatcatat
    10021 aagccatctg gcggtgtcac ttctggtaca atcggtacat tcataacttt gtaactttta
    10081 tgcgctaaat actgggataa tggtgtcttt gaagttctcg aaataccaag taaaacaata
    10141 tcagctttag gtaatccttt aggatcttta ccatcatcgt atttaactgc aaactctatc
    10201 gcatcaattt tcttgaaata tgcatcatct aatctatgaa cgatacctgg ctcattataa
    10261 ggtttttctt caaccgaagc tgataataaa tccattaatg gccccatgat atcgacagac
    10321 ttcaattgga attctgctac tttctcactc atatattgct tcatttcagg tttaataagt
    10381 gtataaacaa tgatagcatt tgtatctttt gcaacttgaa tcacttcatc aacatcttca
    10441 aaagattcaa tatatggata tcttaataat tcatttttac attgcttagg attgaattgt
    10501 gaaatacctg ccctagcaac taactctgcc gtttcaccta tagaatctga agctacgata
    10561 attttaattt tttccattac ttctcaccta ttctttatat aatgctacta ataacttagc
    10621 tattgttgtt ttggaaattc ttccaattac ttcatacttt tgattatctt tttttcttac
    10681 aattggaatc gaatcaattt ctttttcaat cattctatct gctgcgtata tgactaattc
    10741 gctttcctct aaataagtga cattaggcat acgtgtcata tttacactga taggtactgt
    10801 atgaatatct gctccaatca ttgaagctct taataaatct tttcttgaac acacaccaac
    10861 aaaatcgtta tcttcattaa taataaataa tgtacttaca tcttctaaaa aaattgtaca
    10921 aatagcgtca taaactgttg tattctctct tagcacaaca ggttgagaca tatagtcctt
    10981 aacctcaaat tgtcgaagtt tttcattaaa aaacttacct tttgatttac ctgaataata
    11041 atatccaact cggggacgcg cttctaaaaa acctgacatt gttaatatcg ctaaatctgg
    11101 tctaagcgtt gctcttgtta aattcaactt atctgctatt tgttcaccag taatgggtcc
    11161 tttagtttta acaatttcga tgattcgttc ttgtctttga ctgagttcta taggtcttca
    11221 cccctttttt attcacttat tattataccg atatttcttt catgtcacaa acacattaat
    11281 tttacttgcc tttaaataat ctatcaatta gaataaacgt acagcgagtt aaggatagtg
    11341 taagcttaac aataagattg gcgcaacgaa tcattttaaa ataaaagcga gtgactacac
    11401 taatttgggt ggaaccgcgg gttaaatacg taacttattt tgtattatat acaatttaaa
    11461 atattaactc gtcccatgga agtaaagcat atttactatg ctgttatttt ccatggggcg
    11521 ttttcatgta tgaggagagg taattatggc aaaagatatg gatacaattg tttcattagc
    11581 aaaacacaga ggttttgtgt tccctggtag tgatatttac ggtggtttat caaacacatg
    11641 ggattatggt cctttaggtg ttgaattaaa gaataatgtt aaaaaagctt ggtggcaaaa
    11701 attcattaca caatcaccgt ttaacgttgg tatcgatgct gcaatcttaa tgaatccaaa
    11761 agtatgggaa gcttcaggac acttaaacaa cttcaacgac ccaatgattg ataataaaga
    11821 tagtaaaatt cgatatcgcg ctgataaatt aattgaagat tatatgcaag atgttaaagg
    11881 tgatgaaaac ttcattgccg atggtttaag ttttgaacaa atgaaaaaaa ttattgacga
    11941 tgaaggtatt gtttgtcctg taagtaaaac tgctaactgg actgaaattc gccaattcaa
    12001 tttaatgttt aaaacattcc aaggtgtaac tgaagattct acaaatgaaa ttttcttacg
    12061 tcctgaaaca gcacaaggta tttttgtaaa ctataaaaac gtgcaacgtt caatgcgtaa
    12121 aaaattacca tttggtatcg gtcaaattgg taaatcattc cgtaatgaaa tcactccagg
    12181 taacttcatt ttcagaacaa gagaatttga acaaatggaa cttgaattct tctgtaaacc
    12241 tggagaagaa atcgaatggc aaaattattg gaaaactttt gcaagtgact ggttaacaag
    12301 cttaaatatg agcagtgaaa atatgcgttt acgtgatcat gatgaagatg aattatctca
    12361 ttactcaaat gcaacaactg atattgaata taaattccca tttggttggg gtgagttatg
    12421 gggtatcgca agtcgtacag acttcgactt acgtaaacat gctgaacact ctggtgaaga
    12481 tttcagatac catgatccag aaacgaacga aaaatatatt ccatattgta tcgagccatc
    12541 acttggtgca gatcgtgtaa cattagcttt cttatgtgat gcatatgatg aagaaggcgt
    12601 tgaaggtagt aaagatgcac gtacagtttt acacttccat cctgcattag caccatataa
    12661 agcagcgatt ttacctttaa gtaagaaatt atctggcgaa gcgattaaga tttttgagca
    12721 attaagttct aaattctcaa tcgatttcga tgaatcacaa tctatcggta aaagataccg
    12781 tcgtcaagat gaaatcggta caccttattg tgtaacattc gactttgatt cattagaaga
    12841 taatcaagtt acagtacgtg acagagattc aatggaacaa gttcgtatgc caatctcaga
    12901 gttagaagct ttcttaactg aaaaaacaaa attctaatta aaaagactct ccaccatatg
    12961 tggtggctat ataatttata aaaagagata ctggttagta aaattgccag tatctctttt
    13021 ctattcgaaa aaggtattca aataaacaca tctatgtggc tacttgaata ccttttatta
    13081 ttttatgtta ttgttccaat ctttttaatt ggttgattag tttctgactt ttaaaaaaca
    13141 tacctgcata ttcacgatat aacattaaaa tgatatctga catttcatca ataatttctt
    13201 gatggatatt caatgaattc attttatcta tcggtaattt ttgtaataca tctaataaat
    13261 atagtgtttt attcgataat ataactgcat gtacatcttt agaagcctct tgccttgaaa
    13321 tcgcaccgtc aaacttaaaa ctataaccta ttaaatctgc ttgtgtgtca ttaccactca
    13381 cagcacagcg attaaatgat gcagtaaaac caaatcgttt catacacttt aacataacta
    13441 cgactgacat taactgtgca gatgtacctg attctatttt ttcaagaaca aattgtaata
    13501 attgatagtt atatggtgca atgtcacctt catccattga gcgctcaata gtttcagctg
    13561 ccagagacgc ataactgctt acgaaaaggt ccatttgtaa tttataatgt tgactaataa
    13621 catctacaga atttaacgtt cccatacctc gccactgatt gtaaataaac aaaccataaa
    13681 caaacaattg cgtttgcgct tgtaaacccg tcttaacttt tttagcacgc ctagccataa
    13741 gtggtacttt tgcaccatgc tcatttaaaa tcgtgataat tttatcagat tcaccataat
    13801 caactgcttt gatgataatc cctttttggc gcattaacaa ttatcttcac cacttttaag
    13861 attaatcttg gtcttcaaca taaccaattt ggcgaataaa gttaactttg tttcgccagt
    13921 ctctttgaac tttgacccat aattctaagt aaactttaga gcctagaagc atttctatat
    13981 cacgtctcgc acgtttccct acttctttta actttttacc gccttttcca atgacaattc
    14041 ctttttgcga atctctttca acatatatag ttgcttcgat atgaacacga tcttcgcttt
    14101 ctttaaccat acggtccaca ttaacaccaa tcgcatgagg gatttcttca cttgtaagat
    14161 gaaggatttt ttcacgaatg atttcaccca ctacaaattg ttcaggatgg tctgaaattt
    14221 gatcatctgg ataatattta ggtccttcgg gtaaatacgt ctttaaaaca tcaataaaat
    14281 gatctacatt tagcccttct aatgctgaaa taggtacaat ctctgtaaag tccatataac
    14341 tttgatattc ttcaatcttt ggcattaatt catctggatg cactaaatct attttattta
    14401 atactaaaaa tactggtgtc ttaacatttt tcaacatttc tataatatat tcatcacctc
    14461 gtccaatttc ctcattggca ttaaccataa acatgattgc atctatctca gataatgtat
    14521 ttttagcgac tttcatcata tagtcaccta atttgtgttt aggtttatga atacctggcg
    14581 tatcaatgaa tataatttgc gcgtcatctc ttgtcataac accttgaatt ttatttctag
    14641 ttgtttgagc tttatcggac atgattgcta ttttatggcc gatcactcta ttaacaaatg
    14701 ttgacttccc tacatttggt ctacctataa ttgaaacaaa tcctgattta tgttctgtca
    14761 tttattctaa atcctttcct gaaaatccaa atggtagtaa ctctgcgact gtcatcataa
    14821 ccatatctcc tttatgattt gtcatataca caggcatatc atcatcacat aattccttta
    14881 aaacttgacg acatgcacca caaggtgatg acggtttatc tgcatctacg gttacagtta
    14941 ttgattcaaa atcacctggt ctgtatcctt gagaaattgc cgataccaaa ctagctcgtt
    15001 cagcacatat cgataatgga taagaagcat tttctacatt ggtaccataa aaagttctac
    15061 cgtctttcgt ttttaaataa gctcctactt taaattgact gtatggcgaa tatgattctt
    15121 gttgtgcttt tctaacttct tgaaaataat gaggttgata actcatatat gcctccctaa
    15181 aataacgcta taaaatgtgg taaaaatact attaaaccta taataaatgc taatattgaa
    15241 actataagta cactaaaagc cgctatatct tttgcgtatt tagctaaatc atgatattca
    15301 acggtcacta aatcgacaac atattcaata gcagtgttta aagcttcaac agtgagaact
    15361 aatgcaatag caatgagtat aaatatccac tcaatacgat taatatttag tacgagacca
    15421 aagacaatag caacaatcat tgcaaacaca tgtaaaagaa atttatagtc tttttgaatt
    15481 aagattttca gcccatcaag tgcatattta aaccttttca taattagcct cgtgttaatc
    15541 catatgcgtt taatattgta tcttgtcgac caaacatttc cttttcatcc gcttcagtca
    15601 tatgatcata acctaataga tgcaaaaatc catgtaatgc taaaaatcct aattctcgtt
    15661 caaaagaatg tccgtaattg tttgcttgtt cttgcgctac atccgtacag ataattatat
    15721 cccctaaaac acgtggtata tcaagaccac taaaatcaat ctctggctca tcttcttcta
    15781 aagcaaatga gattacatct gtaactttat ctttatctct atatgttcga ttaatttctt
    15841 gtatttcttg tttatctaca aatgtaacag aaagctcagc atcgtcttct atatgctctt
    15901 cttttttagc aaattctaat aaatcttcaa tttgtttata ccacgcatct ttaactaagc
    15961 ctgtgtgatc gctaaaatct atcgtaaaca tttaattctc tccttcataa tgttcaatga
    16021 tcttacttac caatggatgt cttactacat cgctctgatc taatttcaat atacttatac
    16081 ctttaacgtt gtgtaaccta ctgaccgctt ccttaagtcc acttttaaca cctttaggta
    16141 aatcgatttg agtttggtca ccagtaacta ccatttttga gccaaaacct agtcttgtta
    16201 aaaacatttt catttgcgca tgtgtcatat tctgcgcctc atcaagaatt acaaatgcat
    16261 cttctaatgt tcgtccgcgc atatatgcaa gtggcgctat ttcgataatg cctctttcaa
    16321 taaatcgctc tgtttgttca cgcccaagaa cagtatatag accatcatat aaaggtctta
    16381 aatatggatc taccttttct ttcaaatctc ctggtaaaaa tccaagtgac tctcctgctt
    16441 caacagcagg tcttgttaat acaatacgtt taacagcacc tttacggagt tgctttgctg
    16501 cataaactac agctaagaat gtcttacctg taccagcagg acctatacca aatactaaat
    16561 cattattttt catggcatta acatatatac gttgccccat cgttttcgca cgaatcgtct
    16621 taccaaatgc atctttagtt atctcttcat catataaatc taacagatgt tgaattgtgt
    16681 tattatgcgc cattttaata gctgcttcaa catctttaat tgtaatatta ttacctaaat
    16741 caataacctt cagcaaattg attaatactg attccgcttt ttctacgttt tctatttttg
    16801 tacctttaac ggcaacttct tgtcctcttg catggatgac aacatcgaaa ctctcttcaa
    16861 ttgcttttaa atgttcatca ttatttccaa ttaaagcttg agattgattc atatcgtcta
    16921 tttgtataat tccaggcata cacgcgctcc ttttcatata tcatttatat tcattctatg
    16981 atgctttaat atctacttaa ttatatcatg caattgattg atttaaacat ctttgcattg
    17041 acttactcat ttttctttaa tttaaaataa tcatttatac ttttcacctt gtcataaaag
    17101 cagattcaat tttagactaa tcaatttttt gttgtaattt aaataactaa tatttctcga
    17161 aagattctct ttgtttttca atttttctta acgtaaaaaa acaagcaact accattacga
    17221 tagtgcttgt tttcaaaaaa ttttataatt gttttggttt agctaaaatt tctgaccata
    17281 tcataccatt gattacttca tctttatcaa attggaacgc tgatttagtc aaatcttttt
    17341 cgacattttg agaattaagc aactgcttta atttcaaacg ttttgtacgt tcagataagt
    17401 atttatcctc aataatatct ctagcacgtt tttccatttt agcaatttgc ttttcttttt
    17461 ctctatcaat gtcagtacga atcgttttaa tatcatctct aagtgatttt tctaattgac
    17521 gtctaatttc gtcagaatta tcttgtctag aaggtctatg tttctgctcc accggttctg
    17581 ctttaggcac tgtaattggt ttctctgtca tcggttttgt ttctggttgt tttttaggtg
    17641 ccataggttc tacaacaggt ttcgatttag gctcttcctt tggaagttca tcgaataaag
    17701 gtggtaacgt atcatcatat tttcgtttcg atgatttctt ttcttcttca tttaattctt
    17761 cacttatttc tttaaacgtt cgctcaattt cttcaaaaaa gccacctttt tttggttcat
    17821 tatcggtaga tgttttttga ggtggctttt gattttgtct atctttatga ctattttcgc
    17881 gcatagtagt aatgatagaa atgatcactg atatgacaaa aattagaata ccgacactca
    17941 tttaatcacc tctcgactta atgttcaggt gactcatcat cactttgatc agttcgttta
    18001 ttaattgcat ttctcatgcc tgtatcagct tcgatatttt tcaaattata ataatcttta
    18061 acactgatat tacctgaacg taatgcttca gccatagcta atggtacttc agattcggct
    18121 tcaactactt tagcatgcat ttcttgtaca cgcgctttca tttcttgctc agttgctaca
    18181 gccatagctc tacgttcttc agcttttgct tgtgcaatat ttttgtctgc taatgcttgt
    18241 tcagtttgta agtctgcacc aatattttta ctaatatcaa cgtcagcaat atcaattgat
    18301 aaaatttcaa atgcagtacc tgaatctaaa cctttgctta aaactgtttt agaaatatta
    18361 tctgggtttt caagtacttc tgtatgatgc ttactagaac caattgttga aacgatacct
    18421 tcaccaacac gtgcgatgat tgtttcttca ccagcaccac caacaagtcg agcaatatta
    18481 gctctaactg tgatacgagc tttggctttc acttcaatac cgttcattgc tacacctgcg
    18541 ataaatggtg tttcaatgac tttaggatta acagacattt gaaccgcttc taatacgtca
    18601 cgtcctgcaa ggtcaattgc agcagcacgt tcgaaaggaa gatcaatgtc agcacgttgt
    18661 gcagcaatat tagcgtcaac aactctgtca acatttcctc ctgctagata atgcgattct
    18721 aattggtttg ttgttaatgc tagtcctgct ttatgcgctt taattaatgg cgctataact
    18781 tttcttggag atacacgacg taaacgcata ccaaccaatg tacctatacc aacatgaacg
    18841 ccagctgcta acgctgaaat ccataaacca atgggtacaa atgagaataa aataagtaat
    18901 gcaactacta taataactgc tattacgata aaacttaaac taaacatggt atcgctcctt
    18961 ttaattaatc tacttccctc acaactactc ttgttccttc aacttcaagg atttttaccg
    19021 ttttattgcg taaaataaag ttgccatctg aaacagcatc aatacgttca ttttcacaaa
    19081 aaataatccc tgcaggtcga agatctgtaa ctgtttgagc agtctttcct acgaggtgcg
    19141 agcggttatc atgagaattg taacctgact cagaattagt tgaatctttt aagataactt
    19201 tatccaaaaa cggaatcttt ctgttgaaaa tcttcactaa tatcacccat tctacaatcg
    19261 ttaaaatcaa ggcaacgata acattcgcaa gcataaatag caaattatca ccgagcgttg
    19321 ttatgcttat agttatcagt atcatgccaa taataccaat tactgcacca actacaaata
    19381 attcaattac aactaatata acgccaattg agaatattaa gatagaatgc atattgacat
    19441 ttccttggat tagaaatccc aaaaataaaa taagtaatga taatgtggcg ataataccag
    19501 ctgcattgat ttttttagag taaagttgat atacaaatcc taagaatgtc aaacaggtta
    19561 atattaacgt aaaaataggt tgaacaatta tattactaac ttgttcaacc caagtatctc
    19621 cagccgttga ttccaagata gttgtcattt gtaaaaaatt attataactc acaatttcac
    19681 ctcgccctca cattaattat acatgaaatt
//
LOCUS       DAMZKC010000039        18988 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.039, whole genome shotgun sequence.
ACCESSION   DAMZKC010000039 DAMZKC010000000
VERSION     DAMZKC010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 18988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18988
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.039"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            52..1242
                     /locus_tag="POB55_001013"
     CDS             52..1242
                     /locus_tag="POB55_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HDI6499986.1"
                     /translation="MTKSEKIIELTNHYGAHNYLPLPIVISEAEGVWVKDPEGNKYMD
                     MLSAYSAVNQGHRHPKIIQALKDQADKVTLVSRAFHSDNLGEWYEKICKLAGKDKALP
                     MNTGAEAVETALKAARRWAYDVKGIEPNKAEIIAFNGNFHGRTMAPVSLSSEAEYQRG
                     YGPLLDGFRKVDFGDVDALKAAINENTAAVLVEPIQGEAGINIPPEGYLKAIRELCDE
                     HNVLFIADEIQAGLGRSGKLFATDWDNVKPDVYILGKALGGGVFPISVVLADKEVLDV
                     FTPGSHGSTFGGNPLACAASIAALDVIVDEDLPGRSLELGDYFKEQLKQIDHPSIKEV
                     RGRGLFIGVELNESARPYCEALKEEGLLCKETHDTVIRFAPPLIITKEELDLALEKIR
                     HVFQ"
     gene            1351..2595
                     /locus_tag="POB55_001014"
     CDS             1351..2595
                     /locus_tag="POB55_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HDI6499987.1"
                     /translation="MTENNNLVTSTQGIIKEALHKLGFDEGMYDLIKEPLRMLQVRIP
                     VRMDDGTVKTFTGYRAQHNDAVGPTKGGVRFHPDVDEEEVKALSMWMTLKCGIVNLPY
                     GGGKGGIVCDPRQMSIHEVERLSRGYVRAISQFVGPNKDIPAPDVFTNSQIMAWMMDE
                     YSALDKFNSPGFITGKPIVLGGSHGRDRSTALGVVIAIEQAAKRRNMQIEGAKVVIQG
                     FGNAGSFLAKFLYDLGAKIVGISDAYGALHDPNGLDIDYLLDRRDSFGTVTNLFEETI
                     SNKELFELDCDILVPAAISNQITEDNAHDIKASIVVEAANGPTTPEATRILTERGILL
                     VPDVLASAGGVTVSYFEWVQNNQGYYWSEEEVNEKLREKLEAAFDTIYELSQNRKIDM
                     RLAAYIIGIKRTAEAARYRGWA"
     gene            complement(2985..3914)
                     /locus_tag="POB55_001015"
     CDS             complement(2985..3914)
                     /locus_tag="POB55_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDI6499988.1"
                     /translation="MTNSSKSFTKFMAASAVFTMGFLSVPTAGAEQTNQIANKPQAIQ
                     WHTNLTNERFTTIAHRGASGYAPEHTFQAYDKSHNELKASYIEIDLQRTKDGHLVAMH
                     DETVNRTTNGHGKVEDYTLDELKQLDAGSWFNKKYPKYARASYKNAKVPTLDEILERY
                     GPNANYYIETKSPDVYPGMEEQLLASLKKHHLLNNNKLKNGHVMIQSFSDESLKKIHR
                     QNKHVPLVKLVDKGELQQFNDQRLKEIRSYAIGLGPDYTDLTEQNTHHLKDLGFIVHP
                     YTVNEKADMLRLNKYGVDGVFTNFADKYKEVIK"
     gene            complement(4156..5535)
                     /gene="argH"
                     /locus_tag="POB55_001016"
     CDS             complement(4156..5535)
                     /gene="argH"
                     /locus_tag="POB55_001016"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HDI6499989.1"
                     /translation="MSNKAWGGRFEVQPEEWVDDFNASITFDQTLINQDIEGSIAHAT
                     MLANQGIISQQDSEQIIQGLKSIQHDYHQDQIQFSASLEDIHLNIEHELIKRIGDAGG
                     KLHTGRSRNDQVATDMHLYTKKQVQDIIALIKSLQSVIVDIASNNVDTIMPGYTHLQR
                     AQPISFAHHIMTYFWMLQRDQQRFEDSLKRIDINPLGAAALSGTTYPIDRHETTALLN
                     FGSLYENSLDAVSDRDYIIETLHNISLTMVHLSRFAEEIIFWSTDEAKFITLSDAFST
                     GSSIMPQKKNPDMAELIRGKVGRTTGHLMSMLMTLKGLPLAYNKDMQEDKEGLFDAVH
                     TIKGSLRIFEGMIQTMTINKERLNQTVKEDFSNATELADYLVTKNIPFRTAHEIVGKI
                     VLECIQQGHYLLDVPLATYQQHHSSIDADIYDYLQPENCLKRRQSYGSTGQSSVKQQL
                     DVAKQLLSQ"
     gene            complement(5525..6730)
                     /locus_tag="POB55_001017"
     CDS             complement(5525..6730)
                     /locus_tag="POB55_001017"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HDI6499990.1"
                     /translation="MKEKIVLAYSGGLDTSVAVQWLIDKGYDVVACCLDVGEGKDLDI
                     VYKKALDMGAVECHIIDATKEFSDEYVSYAIKGNLMYENAYPLVSALSRPLIAKKLVE
                     IAEKTNSVGIAHGCTGKGNDQVRFEVAIKALNPSLKAFAPVREWAWSREEEIDYAIKH
                     NIPVSINHDSPYSIDQNLWGRANECGILEDPYAAPPEDAFDLTNALEETPDAADEIIL
                     TFDKGIPVQIDGKTYELDDLILTLNALAGKHGIGRIDHVENRLVGIKSREIYEAPAAE
                     VILKAHKALETITLTKDVAHFKPIIEKQFAEQLYNGLWFSPLTDSLKLFIDSTQQYVS
                     GDVRIKLFKGNAIVNGRKSPYTLYDEKLATYTKEDAFNQDAAVGFIDIYGLPTQVNSM
                     LHGGYSNEQ"
     gene            7081..8412
                     /locus_tag="POB55_001018"
     CDS             7081..8412
                     /locus_tag="POB55_001018"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HDI6499991.1"
                     /translation="MTHIQLDFSKTLEFFGEHELKQQQEIVKSIHKTIHEGTGAGSDF
                     LGWVDLPVDYDKEEFSRIVEASKRIKENSDVLVVIGIGGSYLGARAAIEMLTSSFRNS
                     NEYPEIVFVGNHLSSTYTKELVDYLADKDFSVNVISKSGTTTEPAVAFRLFKQLVEER
                     YGKEEAQKRIFATTDKEKGALKQLATNEGYETFIVPDDVGGRYSVLTAVGLLPIATAG
                     INIEAMMIGAAKAREELSSDKLEENIAYQYATIRNILYAKGYTTEMLINYEPSMQYFN
                     EWWKQLFGESEGKDFKGIYPSSANYTTDLHSLGQYVQEGRRFLFETVVKVNHPKYDIT
                     IEKDSDDLDGLNYLAGKTIDEVNTKAFEGTLLAHTDGGVPNMVVNIPQLDEETFGYVV
                     YFFELACAMSGYQLGVNPFNQPGVEAYKQNMFALLGKPGFEDLKKELEERL"
     gene            8737..9312
                     /locus_tag="POB55_001019"
     CDS             8737..9312
                     /locus_tag="POB55_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDI6499992.1"
                     /translation="MSFHQVEEWFEIFRQFGYLPGFILLYIRAIIPVFPLALYILINI
                     QAYGPILGILISWLGLISGTFTVYLICKRLVNTERMQRIKQRTAVQRLISFIDRQGLI
                     PLFILLCFPFTPNTLINFVASLSHIRPKYYFIVLASSKLVSTIILGYLGKEITTILTH
                     PLRGILMLVVLVVFWIVGKKLEQHFMGSKKE"
     gene            9317..9841
                     /gene="lepB"
                     /locus_tag="POB55_001020"
     CDS             9317..9841
                     /gene="lepB"
                     /locus_tag="POB55_001020"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDI6499993.1"
                     /translation="MKKVVKYLISLILAIIIVLFVQTFVIVGHVIPNNDMSPTLNKGD
                     RVIVNKIKVTFNQLNNGDIITYRRGNEIYTSRIIAKPGQSMAFRQGQLYRDDRPVDAS
                     YAKNRKIKDFSLRNFKELDGDIIPPNNFVVLNDHDNNQHDSRQFGLIDKKDIIGNISL
                     RYYPFSKWTIQFKS"
     gene            9857..10432
                     /gene="lepB"
                     /locus_tag="POB55_001021"
     CDS             9857..10432
                     /gene="lepB"
                     /locus_tag="POB55_001021"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDI6499994.1"
                     /translation="MKKELLEWIISIAVAFVILFIVGKFIVTPYTIKGESMDPTLKDG
                     ERVAVNIIGYKTGGLEKGNVVVFHANKNDDYVKRVIGVPGDKVEYKNDTLYVNGKKQD
                     EPYLNYNLKHKQGDYITGTFQVKDLPNANPKSNVIPKGKYLVLGDNREVSKDSRAFGL
                     IDEDQIVGKVSFRFWPFSEFKHNFNPENTKN"
     gene            10592..14068
                     /gene="addB"
                     /locus_tag="POB55_001022"
     CDS             10592..14068
                     /gene="addB"
                     /locus_tag="POB55_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HDI6499995.1"
                     /translation="MTLHAYLGRAGTGKSTKMLTEIKQKMKADPLGDPIILIAPTQST
                     FQLEQAFVNDPELNGSLRTEVLHFERLSHRIFQEVGSYSEQKLSKAATEMMIYNIVQE
                     QQKYLKLYQSQAKYYGFSEKLTEQIQDFKKYAVTPEHLEHFIADKNMQTRTKNKLEDI
                     ALIYREFEQRIQNEFITGEDSLQYFIDCMPKSEWLKRADIYIDGFHNFSTIEYLIIKG
                     LIKYAKSVTIILTTDGNHDQFSLFRKPSEVLRHIEEIANELNISIERQYFNQLYRFNN
                     QDLKHLEQEFDVLQINRVACQGHINILESATMREEINEIARRIIVDIRDKQLRYQDIA
                     ILYRDESYAYLFDSILPLYNIPYNIDTKRSMTHHPVMEMIRSLIEVIQSNWQVNPMLR
                     LLKTDVLTASYLKSAYLVDLLENFVLERGIYGKRWLDDELFNVEHFSKMGRKAHKLTE
                     DERNTFEQVVKLKKDVIDKILHFEKQMSQAETVKDFATAFYESMEYFELPNQLMTERD
                     ELDLNGNHEKAEEIDQIWNGLIQILDDLVLVFGDEPMSMERFLEVFDIGLEQLEFVMI
                     PQTLDQVSIGTMDLAKVDNKQHVFLVGMNDGTMPQPVTASSLITDEEKKYFEQQANVE
                     LSPTSDILQMDEAFVCYIAMTRARQDVTFSYRLMGSSGDDKEISPFLNQIQSLFNQLE
                     ITNIPQYHEVNPLSLMQHAKQTKITLFEALRAWLYDEIVADSWLDAYQVIRDSDHLNQ
                     GLDYLMSALTFDNETVKLGETLSKDLYGKEINASVSRFEGYQQCPFKHYASHGLKLNE
                     RTKYELQNFDLGDIFHSVLKYISERINGDFKQLDLKKIRQLTNEALEEILPKVQFNLL
                     NSSAYYRYLSRRIGAIVETTLSALKYQGTYSKFMPKHFETSFRRKPRTNDELIAQTLT
                     TTQGIPINIRGQIDRIDTYTKNDTSFVNIIDYKSSEGSATLDLTKVYYGMQMQMMTYM
                     DIVLQNKQRLGLTDIVKPGGLLYFHVHEPRIKFKSWSDIDEDKLEQDLIKKFKLSGLV
                     NADQTVIDALDIRLEPKFTSDIVPVGLNKDGSLSKRGSQVADEATIYKFIQHNKENFI
                     ETASNIMDGHTEVAPLKYKQKLPCAFCSYQSVCHVDGMIDSKRYRTVDETINPIEAIQ
                     NININDEFGGEQ"
     gene            14069..17722
                     /gene="addA"
                     /locus_tag="POB55_001023"
     CDS             14069..17722
                     /gene="addA"
                     /locus_tag="POB55_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HDI6499996.1"
                     /translation="MTIPEKPQGVIWTDAQWQSIYATGQDVLVAAAAGSGKTAVLVER
                     IIQKILRDGIDVDRLLVVTFTNLSAREMKHRVDQRIQEASIADPANAHLKNQRIKIHQ
                     AQISTLHSFCLKLIQQHYDVLNIDPNFRTSSEAENILLLEQTIDEVIEQHYDILDPAF
                     IELTEQLSSDRSDDQFRMIIKQLYFFSVANPNPTNWLDQLVTPYEEEAQQAQLIQLLT
                     DLSKVFITAAYDALNKAYDLFSMMDSVDKHLAVIEDERRLMGRVLEGGFIDIPYLTGH
                     EFGARLPNVTAKIKEANEMMVDALEDAKLQYKKYKSLIDKVKSDYFSREADDLKADMQ
                     QLAPRVKYLARIVKDVMSEFNRKKRSKNILDFSDYEHFALQILTNEDGSPSEIAESYR
                     QHFQEILVDEYQDTNRVQEKILSCIKTGDEHNGNLFMVGDVKQSIYKFRQADPSLFIE
                     KYQRFTIDGDGTGRRIDLSQNFRSRKEVLSTTNYIFKHMMDEQVGEVKYDEAAQLYYG
                     APYDESDHPVNLKVLVEADQEHSDLTGSEQEAHFIVEQVKDILEHQKVYDMKTGSYRS
                     ATYKDIVILERSFGQARNLQQAFKNEDIPFHVNSREGYFEQTEVRLVLSFLRAIDNPL
                     QDIYLVGLMRSVIYQFKEDELAQIRILSPNDDYFYQSIVNYINDEAADAILVDKLKMF
                     LSDIQSYQQYSKDHPVYQLIDKFYNDHYVIQYFSGLIGGRGRRANLYGLFNKAIEFEN
                     SSFRGLYQFIRFIDELIERGKDFGEENVVGPNDNVVRMMTIHSSKGLEFPFVIYSGLS
                     KDFNKRDLKQPVILNQQFGLGMDYFDVDKEMAFPSLASVAYRAVAEKELVSEEMRLVY
                     VALTRAKEQLYLIGRVKNDKSLLELEQLSISGEHIAVNERLTSPNPFHLIYSILSKHQ
                     SASIPDDLKFEKDIAQVEDSSRPNVNISIIYFEDVSTETILDNNEYRSVNQLETMQNG
                     NEDVKAQIKHQLDYQYPYVNDTKKPSKQSVSELKRQYETEESGTSYERVRQYRIGFST
                     YERPKFLSEQGKRKANEIGTLMHTVMQHLPFKKERISEVELHQYIDGLIDKHIIEADA
                     KKDIRMDEIMTFINSELYSIIAEAEQVYRELPFVVNQALVDQLPQGDEDVSIIQGMID
                     LIFVKDGVHYFVDYKTDAFNRRRGMTDEEIGTQLKNKYKIQMKYYQNTLQTILNKEVK
                     GYLYFFKFGTLQL"
     gene            17888..18790
                     /locus_tag="POB55_001024"
     CDS             17888..18790
                     /locus_tag="POB55_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HDI6499997.1"
                     /translation="MKFLSFKYNDKTSYGVKVKREDAVWDLTQVFADFAEGDFHPKTL
                     LAGLQQNHTLDFQEQVRKAVVAAEDSGKAEDYKISFNDIEFLPPVTPPNNVIAFGRNY
                     KDHANELNHEVEKLYVFTKAASSLTGDNATIPNHKDITDQLDYEGELGIVIGKSGEKI
                     PKALALDYVYGYTIINDITDRKAQSEQDQAFLSKSLTGGCPMGPYIVTKDELPLPENV
                     NIVTKVNNEIRQDGNTGEMILKIDELIEEISKYVALHPGDIIATGTPAGVGAGMQPPK
                     FLQPGDEVKVTIDNIGTLTTYIAK"
BASE COUNT         6614 a         2798 c         3457 g         6119 t
ORIGIN      
        1 tggataatgt ttcatgtata atagttataa atgatgaaaa ggggagatat gatgactaaa
       61 tctgaaaaaa ttattgagtt aacaaatcat tacggagcac ataattattt accattgcca
      121 attgtcattt cagaagctga aggggtatgg gttaaagatc ctgaaggcaa taaatatatg
      181 gatatgttat ctgcatattc cgctgttaac caaggtcata gacatccgaa aattattcaa
      241 gcattaaaag atcaagctga taaagtgact ctagtttcac gtgcttttca tagtgataac
      301 ttaggtgaat ggtacgaaaa aatttgtaaa ctggcaggta aagataaagc tttaccaatg
      361 aatacaggtg ctgaagcagt agaaacagct ttgaaagcag cacgacgctg ggcatacgat
      421 gttaaaggaa ttgagccaaa taaagcagaa atcattgcat ttaatggtaa cttccatggt
      481 cgaacaatgg cgccagtttc attatcttca gaagcagaat accaacgtgg ttatggtccg
      541 ttattagatg gatttagaaa agttgatttt ggagatgtag atgcattgaa agctgcaatt
      601 aatgaaaata ctgcagcagt tttagtagaa ccaattcaag gtgaagcggg tataaatata
      661 ccgccagaag gatatttgaa agcaattaga gaattatgtg atgaacataa tgtcttattt
      721 attgctgacg aaatccaagc aggattaggt cgttcgggta aattatttgc tacggattgg
      781 gataatgtaa aacctgatgt ctatatttta ggtaaagcac taggtggtgg tgtcttccca
      841 atttctgttg tattagcaga taaagaagta ttagatgtct ttacacctgg ctcacatggt
      901 tcaacatttg gtggtaatcc acttgcttgt gctgcatcaa ttgctgcatt agatgttatc
      961 gttgatgagg atttaccagg gcgctcttta gaattaggag attattttaa agaacaatta
     1021 aagcaaattg atcatccatc aattaaagaa gtccgtggac gtggtttgtt tataggtgtg
     1081 gaacttaatg aaagtgctag accatattgt gaagctttga aagaagaagg cttattatgt
     1141 aaagaaacgc atgatactgt cattcgtttt gcaccaccat taattattac taaagaagaa
     1201 ttggaccttg cacttgaaaa aataagacat gtatttcaat aatgtcaaaa agttgtaaca
     1261 atcagtttgt aagggcttta aaaaggaata cgttatgtta caatatgttt gaaagcgaaa
     1321 tcattaggta aagaagaggc gaaaaggatc atgactgaga acaataattt agtaacttct
     1381 actcaaggaa ttattaaaga agcattgcat aaattgggat ttgacgaagg aatgtacgat
     1441 ttaattaaag aacctttaag aatgttacaa gtgcgtatcc ctgtacgaat ggatgatggc
     1501 acagttaaaa cattcacagg ttaccgtgcg caacataatg atgctgttgg accaacaaaa
     1561 gggggcgtgc gtttccaccc agatgttgat gaagaagaag taaaagcatt atcaatgtgg
     1621 atgactttga aatgtggcat tgtaaactta ccatacggtg gtggtaaggg tggtatcgtt
     1681 tgtgatccac gtcaaatgag cattcatgaa gttgaacgtt tatcacgcgg atatgtaaga
     1741 gcaatttcac aattcgtagg tccgaacaaa gatattccag caccagatgt atttacaaac
     1801 tcacaaatta tggcttggat gatggatgaa tatagtgcat tagataaatt taattcacca
     1861 ggtttcatca caggtaaacc aattgtattg ggtggttctc atggacgcga cagatcaact
     1921 gcactaggtg tagttattgc aattgaacaa gctgcaaaac gtcgtaatat gcaaattgaa
     1981 ggtgccaagg ttgttattca aggtttcggt aatgccggaa gtttcttagc taaattctta
     2041 tatgatttag gtgcaaaaat tgtaggtatc tctgatgctt acggtgcatt acacgatcca
     2101 aatggcttag atatagatta tttattagac cgtcgtgata gttttggtac ggtaacaaat
     2161 ttatttgaag aaacaatctc aaataaagaa ttgtttgaat tagattgtga cattttagta
     2221 ccagcggcta tttcaaacca aattacagaa gacaatgcac atgatattaa agctagtatc
     2281 gttgttgaag ctgctaatgg acctacaaca ccagaagcaa cacgtatttt aactgaacgt
     2341 ggtatattat tagttccaga cgtattagca agtgctggtg gtgtaacggt ttcttacttc
     2401 gaatgggtac aaaataatca aggttattat tggtctgaag aagaagttaa tgaaaagcta
     2461 cgtgaaaaat tagaagcggc atttgatacg atttacgaat tgtctcaaaa ccgaaaaata
     2521 gatatgagac ttgcagcata tatcataggt attaaacgta cagcagaagc agctagatat
     2581 cgtggttggg cataattaat tatcatatgt gatttaacga gcttgggaca gaaaacaaag
     2641 ccctaagctc gttaatttta ttttagcagt agttgactgt aaaacaatgc ccgtgtaaca
     2701 cgctcttttc aaaaatagtc ggggccccaa cacagagaat ttcgaaaaga aattctacag
     2761 gcaatgcaag ttggggatgg gccccaacac agagaaattg gggagccaat ttctacagac
     2821 aatgcaagtt ggggtgggac gacgaaataa attttgcgaa aatatcattt ctgtcccact
     2881 cccaaaaaga cagcagtagg ataatttcaa tttggaaata tcttactgct gttttttata
     2941 gtaatagcta tatttttgta tttatgtttt ctagtttaac attactactt aatgacttct
     3001 ttatatttat cagcgaaatt tgtaaagaca ccatcaacgc catatttatt taatcgtaac
     3061 atatcagctt tttcattcac tgtataagga tgtactataa atcctaagtc ttttaaatga
     3121 tgggtatttt gttcagttaa atctgtataa tcaggaccta atccaatcgc ataagagcgt
     3181 atctctttta agcgttggtc gttaaattgt tgtagttcac ctttatcaac taattttact
     3241 aatggcacat gcttattttg acgatgaatt ttctttaaac tttcgtcaga aaatgattga
     3301 atcattacat gtccattttt taatttattg ttatttaaaa ggtgatgctt tttcaatgaa
     3361 gctaataatt gttcttccat tcctgggtat acatcaggtg actttgtttc aatataatag
     3421 tttgcattcg ggccataacg ttctaaaatt tcatctaaag tgggtacttt agcattttta
     3481 taacttgctc ttgcgtattt tggatatttt ttattaaacc aacttcctgc atctaactgt
     3541 tttaattcat caagggtata atcctcaact ttaccgtgtc catttgttgt acggttaaca
     3601 gtttcatcat gcatagcaac taaatggcca tctttggtac gttgtaaatc aatttcgata
     3661 taagatgctt ttaactcatt atgactctta tcatatgctt gaaacgtatg ctcgggtgca
     3721 tagccacttg cgccacgatg tgcgatagta gtgaatcgct cattcgttaa atttgtatgc
     3781 cattgaatag cctgaggttt atttgcaatt tgatttgttt gttcagcgcc agcagtaggt
     3841 actgataaaa atcccatagt aaaaacagca gaagcagcca taaatttagt gaagcttttc
     3901 gaagagttag tcatagtctt tatcctttca tctctttatt tgtagtctaa caattttaaa
     3961 atactacaaa aacctgcttg tgaaaacgct ttacacattt cttttacaat ttataacatt
     4021 tttgtaatat aaatttaaaa gaattatcaa acattttagg aatcttaaaa atgtgatgtt
     4081 caacaagacg aagagagcat taaaataccc acaatgtaat tttaatagac attgtgggta
     4141 ggtagattaa cgtatttatt gtgatagtaa ttgtttagca acatcaagtt gttgtttgac
     4201 cgatgattga cctgttgaac cgtaactttg acgtcgtttt aaacaatttt caggctgcaa
     4261 ataatcgtaa atatcggcat caatactaga atgatgttgt tgatatgttg ctaaaggaac
     4321 atctaataaa taatgacctt gttgtataca ttctaagacg atttttccta caatttcatg
     4381 tgcagttcta aatggaatat ttttagttac taaataatct gctagttccg ttgcatttga
     4441 aaaatcttct ttaacagttt gattgagtcg ttctttatta attgtcatcg tttgaatcat
     4501 accttcgaaa atacgtaaag aacctttaat tgtatggaca gcatcgaata aaccttcttt
     4561 atcttcctgc atatctttgt tatatgctag aggtaatcct tttaaagtca taagcatgct
     4621 cattaaatga ccagtcgttc gaccaacttt acctctaatt aattctgcca tatcaggatt
     4681 tttcttttgt ggcataatag atgagccagt tgaaaatgca tctgataatg taatgaattt
     4741 agcttcgtct gtggaccaga aaataatttc ctctgcaaag cgtgataagt gaaccatcgt
     4801 taaagaaata ttatgcaatg tttcaataat atagtctctg tcactaacag catctaggct
     4861 attctcatag agactgccaa agttcaacaa tgctgttgtc tcgtgtctat cgatagggta
     4921 tgtggtacca cttaaggctg ctgcacctaa aggattaata tcgattcgtt ttaaactatc
     4981 ttcaaatcgt tgttggtctc gttgtaacat ccaaaaataa gtcataatat gatgtgcaaa
     5041 tgaaattggc tgtgcacgct gtaaatgagt ataaccaggc ataattgtat caacattatt
     5101 ggaagcgatg tctacaatta cactttgtaa cgacttaatt aatgcgatga tatcttgcac
     5161 ttgtttctta gtgtacaagt gcatgtctgt tgcaacttga tcgtttctac tgcgtccagt
     5221 atgcaactta ccaccagcat caccaatacg tttaattaat tcatgttcaa tatttaaatg
     5281 aatatcttct aatgatgcac taaattgaat ttgatcttga tgataatcat gttgaataga
     5341 ttttagtcct tgtataattt gttcgctgtc ttgttgacta ataatgcctt gattcgcaag
     5401 catagttgca tgtgcaatgc tgccttcgat atcttgattt atgagcgttt gatcaaaagt
     5461 aatggatgcg ttaaagtcgt caacccactc ttcaggttgt acttcaaatc taccgcccca
     5521 agctttattg ctcattgcta tagcctccat gtagcatcga atttacttga gtaggtaaac
     5581 catagatatc gataaagccg acagcagcgt cttgattaaa tgcatcttct tttgtataag
     5641 ttgctaattt ttcatcatat aatgtgtaag gtgattttct accatttacg atggcattac
     5701 ctttgaataa tttaattctg acatcaccac ttacgtattg ctgagtacta tcaataaata
     5761 attttaagct atcagttaaa ggtgagaacc aaagtccatt gtatagttgt tcagcaaatt
     5821 gcttctcgat gattggttta aagtgtgcga catctttcgt taacgtaatc gtttctaatg
     5881 ctttatgcgc ttttaaaata acttctgcag caggtgcctc ataaatttct cttgatttga
     5941 tacctacaag tctattttct acatggtcaa ttcttccgat accatgctta ccagctaatg
     6001 cattcaacgt taaaattaaa tcgtctaatt catatgtttt gccatcaatt tgaactggga
     6061 tgcctttatc aaacgttaaa atgatttcat cagcagcatc tggtgtttct tctaaagcat
     6121 ttgttagatc gaacgcatcc tctggtggcg cagcataagg atcttctaaa ataccacatt
     6181 cattcgctct gccccataga ttttgatcga tagaataagg tgaatcatgg ttgattgata
     6241 cagggatatt atgtttaatt gcataatcga tttcttcttc acgactccat gcccactcac
     6301 gtacaggtgc gaatgctttc aatgatgggt ttaatgcttt aatggcaact tcgaaacgta
     6361 cttggtcatt ccctttacca gtacaaccat gtgcaatacc tactgaattt gttttctcag
     6421 caatctctac taatttttta gcgattaatg gtcttgataa agctgaaact aatggatatg
     6481 cattttcata cattaaattt cctttgattg cataacttac atactcatca ctaaattctt
     6541 ttgttgcatc aataatatga cattcaactg ctcccatatc taaagctttt ttataaacga
     6601 tgtctaaatc tttaccttca ccaacatcta ggcaacaagc tacaacgtcg tatcctttgt
     6661 cgataagcca ttgaacggcc acacttgtat ctagtcctcc tgaatatgct aaaacaattt
     6721 tctctttcat aaaattcacc tcattgtagt tatctgaata tttcgtatga acacatgata
     6781 gcaaactttt agtgaaaata aaaccattat tttacatatt tattcataaa atacgataga
     6841 gatagttaga gttgtaagta taaatgctta tttatcaatg tttaaaagta ttttaaaaaa
     6901 catgcaaatt gtataaaaaa tgtttgagtg tttgacgcca gtttgaaatg tttgttgaaa
     6961 aataggtgtg aaacgtcaca taaatatcaa ttttattgat taagtacaaa cttgtgaatt
     7021 gaatcgcaaa cgtaaactta gtaaaattaa gatattaaat gaaacaggag gcttttatag
     7081 atgactcata ttcaattaga ttttagtaaa acgttagaat ttttcggtga acacgaatta
     7141 aaacaacaac aagaaattgt taaatcaatt cacaaaacaa ttcatgaagg tactggtgca
     7201 ggtagtgact tcttaggctg ggttgattta ccagttgatt acgacaaaga agaattttca
     7261 agaattgttg aagcatcaaa acgcattaaa gaaaattctg atgttttagt agtcatcggt
     7321 attggtggtt cttacttagg tgcacgtgca gcaatcgaaa tgttaacgtc atcatttaga
     7381 aacagcaatg aataccctga aattgtattt gttggtaatc acttatcatc aacatatacg
     7441 aaagagttag ttgattattt agcagacaaa gatttctctg taaacgttat ttctaaatct
     7501 ggtacaacta cagaaccagc agttgcattt agattgttca aacaattagt tgaagaaaga
     7561 tacggtaaag aagaagcaca aaaacgtata tttgcaacaa cggataaaga aaaaggtgct
     7621 ttaaaacagt tggctacaaa cgaaggttat gaaacgttta tcgtacctga tgatgtaggt
     7681 ggaagatatt ctgttttaac agcagtagga ttattaccaa ttgcaactgc tggaattaac
     7741 atcgaagcta tgatgattgg tgctgcaaaa gcacgtgaag aattatcttc agataaatta
     7801 gaagaaaaca ttgcatacca atatgcgaca attcgaaaca ttttatatgc aaaaggttat
     7861 acaacagaaa tgttgattaa ctatgaacca tctatgcaat actttaatga atggtggaaa
     7921 caattatttg gtgaatcaga aggtaaagac ttcaaaggta tctatccttc aagtgccaac
     7981 tacacaactg atttacattc tttaggtcaa tatgtacaag aaggccgtcg tttcttattc
     8041 gaaacagtgg taaaagtaaa tcatcctaaa tatgatatta ctattgaaaa agatagtgat
     8101 gatctagacg gattaaatta tttggctggt aaaacaatcg acgaagttaa cacaaaagca
     8161 ttcgaaggta cattattagc gcatactgat ggtggtgttc ctaacatggt agtgaacatt
     8221 ccacaattag atgaagaaac tttcggttat gtcgtatact tcttcgaact tgcttgtgca
     8281 atgagtggat accaattagg tgtaaatcca tttaaccaac ctggtgtaga agcatataaa
     8341 caaaacatgt tcgcattatt aggtaaacct ggttttgaag acttgaaaaa agaattagaa
     8401 gagcgtttat aaaatacatt acttcaatga ttagtgaagt ttgaaaagat agaactagac
     8461 gttaactatt taaagcatat tttcgaggtt gtcattacaa atgtaaaaat gtaatgacaa
     8521 cctcgttttt atttatatgc aagaactagg ttactagcta atgtgacaag atgttaagag
     8581 aaaattaaag aaaaaataac atctgtcata caataatatt gttatactac tagagactga
     8641 tttattagca tgattacatg ttaatgtttc tttacttagt aattaacttt ataatgtaag
     8701 aataattatc ttcaaccaaa gaaagggatt gatgatttgt cgtttcatca agtagaagaa
     8761 tggtttgaga tatttcgaca gtttggttat ttacctggat ttatattgtt atatattaga
     8821 gcgataattc cagtatttcc tttagcactc tatattttaa ttaacattca agcttatgga
     8881 cctattttag gtatattgat tagttggctt ggattaattt ctggaacatt tacagtctat
     8941 ttgatctgta aacgattggt gaacactgag aggatgcagc gaattaaaca acgtactgct
     9001 gttcaacgct tgattagttt tattgatcgc caaggattaa tcccattgtt tattttactt
     9061 tgttttcctt ttacgccaaa tacattaata aattttgtag cgagtctatc tcatattaga
     9121 cctaaatatt atttcattgt tttggcatca tcaaagttag tttcaacaat tattttaggt
     9181 tatttaggta aggaaattac tacaatttta acgcatcctt taagagggat attaatgtta
     9241 gttgtgttgg ttgtattttg gattgttgga aaaaagttag aacagcattt tatgggatcg
     9301 aaaaaggagt gacatcgtga aaaaagttgt aaaatatttg atttcattga tacttgctat
     9361 tatcattgta ctgttcgtac aaacttttgt aatagttggt catgtcattc cgaataatga
     9421 tatgtcacca acccttaaca aaggggatcg tgttattgta aataaaatta aagttacatt
     9481 taatcaattg aataatggtg atatcattac atataggcgt ggtaacgaga tatatactag
     9541 tcgaattatt gccaaacctg gtcaatcaat ggcgtttcgt cagggacaat tataccgtga
     9601 tgaccgaccg gttgacgcat cttatgccaa gaacagaaaa attaaagatt ttagtttgcg
     9661 caattttaaa gaattagatg gagatattat accgcctaac aattttgttg tgctaaatga
     9721 tcatgataac aatcagcatg attctagaca atttggttta attgataaaa aggatattat
     9781 tggtaatata agtttgagat attatccttt ttcaaaatgg acgattcagt tcaaatctta
     9841 aaaagaggtg tcaaaattga aaaaagaatt attggaatgg attatttcaa ttgcagtcgc
     9901 ttttgtcatt ttatttatag taggtaaatt tattgttaca ccatatacaa ttaaaggtga
     9961 atcaatggat ccaactttga aagatggcga gcgagtagct gtaaacatta ttggatataa
    10021 aacaggtggt ttggaaaaag gtaatgtagt tgtcttccat gcaaacaaaa atgatgacta
    10081 tgttaaacgt gtcatcggtg ttcctggtga taaagtagaa tataaaaatg atacattata
    10141 tgtcaatggt aaaaaacaag atgaaccata tttaaactat aatttaaaac ataaacaagg
    10201 tgattacatt actgggactt tccaagttaa agatttaccg aatgcgaatc ctaaatcaaa
    10261 tgtcattcca aaaggtaaat atttagttct tggagataat cgtgaagtaa gtaaagatag
    10321 ccgtgcgttt ggcctcattg atgaagacca aattgttggt aaagtttcat ttagattctg
    10381 gccatttagt gaatttaaac ataatttcaa tcctgaaaat actaaaaatt aatatgaaac
    10441 aaatacaaca tcgtttgtcg gttttaatac tgataaacga tgttttattt tgttagtacc
    10501 acaataaaag ctaagttcga aatgaactta taataaatca atcacaatca ctttgtgtta
    10561 aaatatgtgt caaaggaagt gagggtttgt catgacatta catgcttatt taggtagagc
    10621 gggaacaggt aagtctacga aaatgttgac cgaaataaaa caaaaaatga aagcagatcc
    10681 gcttggagat ccaatcattt taattgcgcc aactcaaagt acatttcaat tagaacaagc
    10741 ctttgtcaat gatccggaat taaatggtag tttaagaaca gaagtgttgc attttgaacg
    10801 attaagtcat cgtattttcc aagaagttgg tagttatagc gaacaaaagt tatctaaagc
    10861 tgcaacggaa atgatgattt ataacattgt tcaagaacaa caaaagtatt taaaacttta
    10921 tcaatcacaa gcaaaatatt atgggtttag tgaaaaatta acagaacaaa ttcaagattt
    10981 taaaaaatat gcagtaacgc ctgaacattt agaacacttt attgctgata aaaatatgca
    11041 aactcgaact aaaaataagt tagaggatat tgctttaata taccgtgagt tcgaacaacg
    11101 cattcaaaac gagtttatta ctggtgagga ttcattacaa tattttattg attgtatgcc
    11161 gaaatcagag tggctaaaac gtgctgatat atatattgat ggttttcaca acttttcaac
    11221 gattgagtat ttaataatca aaggattaat taaatatgcg aagagtgtca caattatatt
    11281 gacgacagat ggtaaccacg atcaatttag tttatttaga aaaccatcgg aagtgttacg
    11341 acatattgaa gaaatagcaa atgaactcaa tatttctatt gaacgtcaat atttcaacca
    11401 attatatcgc ttcaataatc aagatttaaa gcatcttgaa caagaatttg atgtacttca
    11461 aatcaatcga gtggcatgtc aaggtcatat caatatttta gaatctgcga ctatgagaga
    11521 ggaaataaat gaaattgcgc gacgtatcat cgttgatatt cgtgataagc aattacgata
    11581 tcaagatatt gctattttat atcgtgatga atcttatgct tatttatttg attccatatt
    11641 accgctttat aatattcctt ataatattga tacaaagcgt tcgatgacac atcatccggt
    11701 catggaaatg attcgttcat tgattgaagt tattcaatct aattggcaag tgaatccaat
    11761 gctacgctta ttgaagactg atgtgttaac ggcatcatat ctaaaaagtg catacttagt
    11821 tgatttactt gaaaattttg tacttgaacg tggtatatac ggtaaacgtt ggttagatga
    11881 tgagctattt aatgtcgaac attttagcaa aatggggcgt aaagcgcata aactgaccga
    11941 agatgaacgt aacacatttg aacaagtcgt taagttaaag aaagatgtca ttgataaaat
    12001 tttacatttt gaaaagcaaa tgtcacaagc ggaaactgta aaagattttg caactgcttt
    12061 ttatgaaagt atggaatatt tcgaactgcc aaatcaattg atgacagagc gagatgaact
    12121 tgatttaaat ggtaatcatg aaaaggcgga ggaaattgat caaatatgga atggcttaat
    12181 tcaaatcctt gatgacttag ttctagtatt tggagatgaa ccaatgtcga tggaacgttt
    12241 cttagaagta tttgatattg gtttagaaca attagaattt gttatgattc cgcaaacatt
    12301 ggaccaagta agtattggta cgatggattt ggctaaagtc gataataagc aacatgtttt
    12361 cttagtaggt atgaatgatg gtacgatgcc acaaccagta actgcgtcaa gcttgattac
    12421 agatgaagaa aagaaatact ttgaacagca ggctaatgtc gagttaagtc caacatcaga
    12481 tattttacag atggatgaag catttgtttg ctatattgct atgacaagag cgcgacaaga
    12541 tgttacattt tcttacagac taatgggatc aagtggtgat gataaggaga tcagcccatt
    12601 tttaaatcaa attcaatcat tgttcaacca attggaaatt actaacattc ctcaatacca
    12661 tgaagttaac ccattgtcac taatgcaaca tgctaagcaa accaaaatta cattatttga
    12721 agcattgcgt gcttggttat atgatgaaat tgtggctgat agttggttag atgcttatca
    12781 agtaattaga gatagcgatc atttaaatca aggtttagat tatttaatgt cagcattaac
    12841 gtttgacaat gaaactgtaa aattaggtga aacgttgtct aaagatttat atggtaagga
    12901 aatcaatgcc agtgtatccc gttttgaagg ttatcaacaa tgcccattta aacactatgc
    12961 gtcacatggt ctgaaactaa atgagcgaac gaagtatgaa cttcaaaact ttgatttagg
    13021 tgatattttc cattctgttt taaaatatat atctgaacgt attaatggcg attttaaaca
    13081 attagacctg aaaaaaataa gacaattaac gaatgaagca ttggaagaaa ttttacctaa
    13141 agttcagttt aatttattaa attcttcagc ttactatcgt tatttatcaa gacgcattgg
    13201 cgctattgta gaaacaacac taagcgcatt aaaatatcaa ggcacgtatt caaagtttat
    13261 gccaaaacat tttgagacaa gttttagaag gaaaccaaga acaaatgacg aattaattgc
    13321 acaaacatta acgacaactc aaggtattcc aattaatatt agagggcaaa ttgaccgtat
    13381 cgatacgtat acaaagaatg atacaagttt tgttaatatc attgactata aatcctctga
    13441 aggtagtgcg acacttgatt taacgaaagt atattatggt atgcaaatgc aaatgatgac
    13501 atacatggat atcgttttac aaaataaaca acgccttgga ttaacagata ttgtgaaacc
    13561 aggtggatta ttatacttcc atgtacatga acctagaatt aaatttaaat catggtctga
    13621 tattgatgaa gataaactag aacaagattt aattaaaaag tttaagttga gtggtttagt
    13681 taatgcagac caaactgtta ttgatgcatt ggatattcgt ttagaaccta aattcacttc
    13741 agatattgta ccagttggtt tgaataaaga tggctctttg agtaaacgag gcagccaagt
    13801 ggcagatgaa gcaacgattt ataaattcat ccaacataac aaagagaatt ttatagaaac
    13861 agcttcaaat attatggatg gacatactga agttgcacca ttaaagtaca aacaaaaatt
    13921 gccatgtgct ttttgtagtt atcaatcggt atgtcatgta gatggcatga ttgatagtaa
    13981 gcgatatcga actgtagatg aaacaataaa tccaattgaa gcaattcaaa atattaacat
    14041 taatgatgaa tttgggggtg agcaatagat gacaattcca gagaaaccac aaggcgtgat
    14101 ttggactgac gcgcaatggc aaagtattta cgcaactgga caagatgtac ttgttgcagc
    14161 cgcggcaggt tcaggtaaaa cagctgtact agttgagcgt attatccaaa agattttacg
    14221 tgatggcatt gatgtcgatc gacttttagt cgtaacgttt acaaacttaa gcgcacgtga
    14281 aatgaagcat cgtgtagacc aacgtattca agaggcatcg attgctgatc ctgcaaatgc
    14341 acacttgaaa aaccaacgca tcaaaattca tcaagcacaa atatctacac tccatagttt
    14401 ttgcttgaaa ttaattcaac agcattatga tgtattaaat attgacccga actttagaac
    14461 aagcagtgaa gctgaaaata ttttattatt agaacaaacg atagatgagg tcatagaaca
    14521 acattacgat atccttgatc ctgcttttat tgaattaaca gagcaattgt cttcagatag
    14581 aagtgatgat cagtttcgaa tgattattaa acaattgtat ttctttagcg ttgcaaatcc
    14641 aaatcctaca aattggttgg atcaattggt gacaccatac gaagaagaag cacaacaagc
    14701 gcaacttatt caactactaa cagacttatc taaagtattt atcacagctg cttatgatgc
    14761 tttaaataag gcgtatgatt tgtttagtat gatggatagc gtcgataaac atttagctgt
    14821 tatagaagat gaacgacgtt taatggggcg tgttttagaa ggtggcttta ttgatatacc
    14881 ttatttaact ggtcacgaat ttggcgcgcg tttgcctaat gtaacagcga aaattaaaga
    14941 agcaaatgaa atgatggtcg atgccttaga agatgctaaa cttcagtata aaaaatataa
    15001 atcattaatt gataaagtga agagtgatta cttttcaaga gaagctgatg atttgaaagc
    15061 tgatatgcaa caattggcgc cacgagtaaa gtaccttgcg cgtattgtga aagatgttat
    15121 gtcagaattc aatcgaaaaa agcgtagcaa aaatattttg gatttttctg attatgaaca
    15181 ttttgcatta caaattttaa caaatgagga tggttcgcct tcagaaattg ccgaatcata
    15241 ccgtcaacac ttccaagaaa tattggtcga tgagtatcaa gatacgaacc gagttcaaga
    15301 gaaaatacta tcttgcatca aaacgggtga tgaacataat ggtaatttat ttatggttgg
    15361 agatgttaag caatccattt ataaatttag acaagctgat ccaagtttat ttattgaaaa
    15421 gtatcaacgc tttactatag atggagatgg cactggacgt cgaattgatt tgtcgcaaaa
    15481 cttccgttct cgaaaagaag tactgtcaac gactaactat atattcaaac atatgatgga
    15541 tgaacaagtc ggtgaagtaa aatatgatga agcggcacag ttgtattatg gtgcaccata
    15601 tgatgaatcg gaccatccag taaacttaaa agtccttgtt gaagcggatc aagaacatag
    15661 tgatttaact ggtagtgaac aagaagcgca ttttatagta gaacaagtta aagatatctt
    15721 agaacatcaa aaagtttatg atatgaaaac aggaagctat agaagtgcga catacaagga
    15781 tatcgttatt ctagaacgca gctttggaca agctcgcaat ttacaacaag cctttaaaaa
    15841 tgaagatatt ccattccatg tgaatagtcg tgaaggttac tttgaacaaa cagaagtccg
    15901 cttagtatta tcatttttaa gagcgataga taatccatta caagatattt atttagttgg
    15961 gttaatgcgc tccgttatat atcagttcaa agaagacgaa ttagctcaaa ttagaatatt
    16021 gagtccaaat gatgactact tctatcaatc gattgtaaat tacattaatg acgaagcagc
    16081 agatgcaatt ttagttgata aattaaaaat gtttttatca gatattcaaa gttaccaaca
    16141 atatagtaaa gatcatccgg tgtatcagtt aattgataaa ttttataatg atcattatgt
    16201 tattcaatac tttagtggac ttattggtgg acgtggacga cgtgcaaatc tttatggttt
    16261 atttaataaa gctatcgagt ttgagaattc aagttttaga ggtttatatc aatttattcg
    16321 ttttatcgat gaattgattg aaagaggcaa agattttggt gaggaaaatg tagttggtcc
    16381 aaacgataat gtcgttagaa tgatgacaat tcatagtagt aaaggtctag agtttccatt
    16441 tgtcatttat tctggattgt caaaagattt taataaacgt gatttgaaac aaccagttat
    16501 tttaaatcag caatttggtc tcggaatgga ttattttgat gtggataaag aaatggcatt
    16561 tccatcttta gcttcggttg catatagagc tgttgccgaa aaagaacttg tgtcagaaga
    16621 aatgcgatta gtctatgtag cattaacaag agcgaaagaa caactttatt taattggtag
    16681 agtgaaaaat gataaatcat tactagaact agagcaattg tctatttctg gtgagcacat
    16741 tgctgtcaat gaacgattaa cttcaccaaa tccgttccat cttatttata gtattttatc
    16801 taaacatcaa tctgcgtcaa ttccagatga tttaaaattt gaaaaagata tagcacaagt
    16861 tgaagatagt agtcgtccga atgtaaatat ttcaattata tactttgaag atgtgtctac
    16921 agaaaccatt ttagataata atgaatatcg ttcggttaat caattagaaa ctatgcaaaa
    16981 tggtaatgag gatgttaaag cacaaattaa acaccaactt gattatcaat atccatatgt
    17041 aaatgatact aaaaagccat caaaacaatc tgtttctgaa ttgaaaaggc aatatgaaac
    17101 agaagaaagt ggcacaagtt acgaacgagt aagacaatat cgtatcggtt tttcaacgta
    17161 tgaacgacct aaatttctaa gtgaacaagg taaacgaaaa gcgaatgaaa ttggtacgtt
    17221 aatgcataca gtgatgcaac atttaccatt caaaaaagaa cgcatatctg aagttgagtt
    17281 acatcagtat atcgatggat taatcgataa acatattatc gaagcagatg cgaaaaaaga
    17341 tatccgtatg gatgaaataa tgacatttat caatagtgag ttatattcga ttattgctga
    17401 agcagagcaa gtttatcgtg aattaccgtt tgtagttaac caagcattag ttgaccaatt
    17461 gccacaagga gacgaagacg tctcaattat tcaaggtatg attgacttaa tctttgttaa
    17521 agatggtgtg cattattttg tagactataa aaccgatgca tttaatcgtc gccgtgggat
    17581 gacagatgaa gaaattggta cacaattaaa aaataaatat aagatacaga tgaaatatta
    17641 tcaaaatacg cttcaaacga tacttaataa agaagttaaa ggttatttat acttcttcaa
    17701 atttggtaca ttgcaactgt agtattttga ttttcaaaag aataaaaaat aatttcgatt
    17761 aagtgcaaag cccttgtagc agaatgaaca taactcattt tcaaaattgt cttacttatt
    17821 tatttgttat ttgataacga aaaaagttat aatgtgaatt aagataaaga tgaggagttg
    17881 agaatgaatg aaattcttat cattcaagta taatgacaaa acttcatatg gcgttaaagt
    17941 aaaacgcgaa gatgctgtat gggatttaac acaagtattt gctgactttg cagaaggaga
    18001 tttccatcct aaaacattgt tagctggttt acaacaaaat catactttag attttcaaga
    18061 acaagtacgt aaagcagttg tagcagcaga agatagcggc aaagctgaag actataaaat
    18121 ttcatttaat gacattgaat tcttaccacc agtaacacct ccgaataatg tgattgcttt
    18181 tggtagaaat tacaaagatc atgcgaacga attaaatcat gaagtagaaa aattatatgt
    18241 atttacaaaa gcagcgtcat ctttaacagg agataatgca acaattccaa atcataaaga
    18301 tattactgat caattagatt atgaaggtga attaggtatt gttattggta agtctggtga
    18361 aaagattcca aaagcattag ctttagatta tgtttacggc tatacaatta ttaacgatat
    18421 cactgatcgc aaagcacaaa gtgaacaaga tcaagcattt ttatcaaaaa gtttaactgg
    18481 cggttgccca atgggtcctt atatcgttac taaagacgaa ctaccattac ctgaaaatgt
    18541 aaatattgtt acaaaagtta acaatgaaat tagacaagat ggtaacactg gcgaaatgat
    18601 tcttaaaatt gatgaattaa tagaagaaat ttcaaaatat gttgcactac atccgggaga
    18661 tattattgca actggtacac cagcaggcgt tggtgcaggt atgcaaccac ctaaattttt
    18721 acaaccaggt gatgaagtta aagtgactat tgataatatt ggaacgctga caacttatat
    18781 cgctaaataa ttatcattta aaaagctaac cagtctttat atagattggt tagttttttc
    18841 ttgcttttct aaaaaggtgt taaagataaa ttatttataa tgttaccatt ttgagatgaa
    18901 agtgaaatat tgatattaag aagtagttga ttattttaca gcagattcac aatattctaa
    18961 taaggaataa aatgtcatgt tcttcctc
//
LOCUS       DAMZKC010000040        18874 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.040, whole genome shotgun sequence.
ACCESSION   DAMZKC010000040 DAMZKC010000000
VERSION     DAMZKC010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 18874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18874
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.040"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(104..568)
                     /gene="bstA"
                     /locus_tag="POB55_001025"
     CDS             complement(104..568)
                     /gene="bstA"
                     /locus_tag="POB55_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol transferase BstA"
                     /protein_id="HDI6499998.1"
                     /translation="MTTKTVFDVIDMGLGYLVNVYDTWKVEKILDDYHKPFSNTIHWQ
                     FGHVLTIFESALAVTGKENIDLNIYRPLFGNGSSPDEWKGEVPSIGTILEGLQTLPER
                     ARKLTEDDLAIELKQPIVGCNNLEELLVLNAIHIPLHAGKIEEMSRILKNLK"
     gene            complement(672..1310)
                     /gene="pcp"
                     /locus_tag="POB55_001026"
     CDS             complement(672..1310)
                     /gene="pcp"
                     /locus_tag="POB55_001026"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HDI6499999.1"
                     /translation="MHILVTGFAPFDNQNINPSWEAVTQLEDIIGTHTIDKLKLPTSF
                     KKVDNIINKTLASNHYDVVLAIGQAGGRNAITPERVAINIDDARIPDNDDFQPIDQAI
                     HLDGAPAYFSNLPVKAMTQSIINQGLPGALSNSAGTFVCNHTLYHLGYLQDKHYPHLR
                     FGFIHVPYIPEQVIGKPDTPSMPLEKIVAGLTAAIEAISNDEDLHLALGTTE"
     gene            complement(1462..2418)
                     /locus_tag="POB55_001027"
     CDS             complement(1462..2418)
                     /locus_tag="POB55_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HDI6500000.1"
                     /translation="MNYQVLLYYKYTTIDDPEQFAQDHLAFCKAHHLKGRILVSTEGI
                     NGTLSGTKEETEQYMAHMHADERFKDMVFKIDEAEGHAFKKMHVRPRKEIVALDLEDD
                     VDPRHTTGQYLSPVEFRKALEDDDTVIIDARNDYEFDLGHFRGAIRPNITRFRDLPDW
                     IKENKALFADKKVVTYCTGGIRCEKFSGWLLKEGFEDVAQLHGGIATYGKDPETKGEY
                     WDGKMYVFDDRISVNINQVEKTIIGKDWFDGKPCERYINCANPECNKQILVSEENETK
                     YLGACSYECAKHERNRYVQANNISDNEWQQRLTNFDDLHQHA"
     gene            2662..3633
                     /locus_tag="POB55_001028"
     CDS             2662..3633
                     /locus_tag="POB55_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HDI6500001.1"
                     /translation="MSQQDLPTLFYSGKSNSAVPIISESELQTITAEPWLEISKKGLQ
                     LEGLNFDRQGQLFLLDVFEGNIFKINPETKEIKRPFVSHKANPAAIKIHKDGRLFVCY
                     LGDFKSTGGIFAATENGDNIQDIIEDFSTTYCIDDMVFDSKGGFYFTDFRGYSTNPLG
                     GVYYVAPDFRTVTPIIQNISVANGIALSKDEKVLWVTETTANRLHRIALEDDGVTIQP
                     FGATIPYYFTGHEGPDSCCIDSDDNLYVAMYGQGRVLVFNKRGYPIGQILIPGRDEGH
                     MLRSTHPQFIPDTNQLIICSNDIEMGGGSMLYTVNGFAKGHQSFQFQ"
     gene            3838..4002
                     /locus_tag="POB55_001029"
     CDS             3838..4002
                     /locus_tag="POB55_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500002.1"
                     /translation="MAIYIFCTILVLVAVGALLTNRIRDKKDKRLDILSSVLLLISSI
                     SLLLYGVIIN"
     gene            complement(4113..4628)
                     /locus_tag="POB55_001030"
     CDS             complement(4113..4628)
                     /locus_tag="POB55_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenoid-binding protein"
                     /protein_id="HDI6500003.1"
                     /translation="MTNFTFDGAHSSLEFQIKHLMVSKVKGSFDQFDVAVEGDINDFS
                     TLKATATIIPSSINTKNEARDNHLKSGDFFGTDEFDKITFVTKSVSESKVVGDLTIKG
                     ITNEETFDVEFNGVSKNPMDGSQVTGVIVTGTINRENYGINFNQALETGGVMLGKDVK
                     FEASAEFSISE"
     gene            5008..5856
                     /locus_tag="POB55_001031"
     CDS             5008..5856
                     /locus_tag="POB55_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HDI6500004.1"
                     /translation="MGNYLVLQSDFGLSDGAVSAMYGVAYTVSDDIRVENLTHDIPPY
                     DIWVASYRLYQTVKYWPKGTVFVSVVDPGVGSDRRSIACLTYSGHYIITPDNGSLSHI
                     KHYEGIKKVIEIDEVKSRLPHSEESHTFHGRDVYAYNGARLAANKIAFERLGQAINVD
                     SIEALEIREATKNEDSVTGSIDILDIRFGSLWTNIPLAFLKSLEIVHGNNLVVTIYHQ
                     DKKVYQNIIKFARSFADVNVGEPLVYVNSLVNIGVAVNQDSFSDLYHIGTGNDWTIHL
                     SKAPSI"
     gene            5885..6439
                     /locus_tag="POB55_001032"
     CDS             5885..6439
                     /locus_tag="POB55_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HDI6500005.1"
                     /translation="MKKQDISVKTVVAIGIGAAVFVILGRFVVIPTGFPNTNIETSYA
                     FLALISAIFGPFAGLMTGLVGHAIKDFTTYGSAWWSWVICSGIIGCLYGWIGLKLNLS
                     SGRFSRKSMIYFNIGQIIANIICWALIAPTLDILIYNEPANKVYTQGVISAVLNIISV
                     GIIGTILLKAYASSQIKKGSLRKE"
     gene            6492..8204
                     /locus_tag="POB55_001033"
     CDS             6492..8204
                     /locus_tag="POB55_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6500006.1"
                     /translation="MTEPIISFKDFSFQYHSQATPTLQKINVDIYPGEKVLVVGASGS
                     GKSTFANCINGLIPFKTKGNITGELYINNQDATVSCLHDRSNVVGTVLQDTDGQFIGL
                     TAAEDMAFLLENNCVEQDDMKKNVSYWAEKVGMIEHLNHRPQDLSGGQKQRVSLGGIL
                     IHRTPILILDEPLANLDPATGHETLRLLNNIHEETKSTMIIVEHRLEESLDDTFDRVL
                     LFKDGKIIANTTPSDLLKSSKLKEAGIREPLYCTALKYAEVDVESIDNLANLRDVCMS
                     EHVKFKVKKWIDETSANNDNKYKSEPLLELNEVCVQYSDYSNSVLNNVQLNVYRREML
                     SIVGHNGAGKSTLAKAICGFLDITGNIQFCNRGFNQLSISERSEFVGYVMQNPNHMIS
                     EKMIYDEVALGLRARGMKESDIKIRVENVLKICGLYAFRNWPIVALSYGQKKRVTIAS
                     VLVLNPEIIILDEPTAGQDFYHYNEIMSFLIELNRQGKTIIMITHDMHLLSEYSSRTV
                     VLSKGQVVADTTPVLVLNDKKICEIASLRQTSLFEMAEYIGISEPQKLVQLFINHDRK
                     VRRQ"
     gene            8201..9034
                     /locus_tag="POB55_001034"
     CDS             8201..9034
                     /locus_tag="POB55_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDI6500007.1"
                     /translation="MNQYNTIGFHPGNSRIHQLNATVKLLFLLVVSISAMVTYDTRYL
                     ILISVSSILLFKYAHIEWKQVRFVVKFILFFTILNIIAVYIFDPEYGVKIYNQRTELI
                     NGIGRFTLTSQELFYLFNLILKYISTVPLALIFLFTTNPSHFAASLNQLGVNYKISYA
                     VSLALRYIPDIQETYFNISQAQQARGYDNSKKAKFTSRVKGIKRIVLPLIFSSIERID
                     TISTAMELRQFGQYKRRTWYVKKQLKKDDYVVLCLTLILLMLVVTLFFLNNSRYFNPW
                     Y"
     gene            9081..9578
                     /locus_tag="POB55_001035"
     CDS             9081..9578
                     /locus_tag="POB55_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6500008.1"
                     /translation="MINTERLNLMIPSSSHLIELYNICSHPQANIYTPKGLHNSKLDT
                     QRWIEKWRNHWQQYQFGYFVLVKKIDCSVIGICGYEYRQLKQETVLNLFYKLHPSFEG
                     QGYACEAITAITNFVNYIDQETVKVIRTNKCNQRSINLAERLKFKRDKAMDDIINQGD
                     IVFYK"
     gene            9696..10820
                     /locus_tag="POB55_001036"
     CDS             9696..10820
                     /locus_tag="POB55_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDI6500009.1"
                     /translation="MHKKYLLLFFLLLSVALLYSCSKQNEKAQNKSVNPNEYAKHKSK
                     MDEHWETYHGQVYHVFYHPLITDPKVAFTGDTHQAKGNNDWMITVSEFKKSLNELYKN
                     NYIIVNPHDVYDLSSHHVKAKKIKLPKGKKPLIISIDDMNYYSYMRHHGYADRLVLDK
                     NKHVVSETTDKKGHATYSDDNDIVPILNKFVKEHPDFSLNGEKGVVALTGYEGVLGYR
                     TNELTSKDYTKNKKAAEEVVRAMKRDGWSFASHSYGHINFEKTSLEGIKRDTKRWKDE
                     VEPIVGKTDMFVFPHGAQDRHTQAYDYLVDEAEFKFIAGVGPNNFTDISATNVYQDRV
                     AIDGLNLFEFKYKLKPFFNPENVYSKQDRQFFKGNRDYEE"
     gene            11124..11942
                     /locus_tag="POB55_001037"
     CDS             11124..11942
                     /locus_tag="POB55_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HDI6500010.1"
                     /translation="MNNSEQLIALKESETAFLKYFNKADYELVDFSVVEKLDWKQLNH
                     EDLQQMGERNFWQHEHQIYALRNDFTDQLLRYYSMYPTAATKVAYTGLIIRNNEAAVQ
                     VGLENYAPSLANVQQSLKLFIQFIQQQLRDNVHFVVLGHYQLLDALLDKSLQTPDILS
                     MIEERNLSGLVTYLSTEHPIVQILKENTQQQLNVLEHYIPNDHPALVELKIWERWLHT
                     QGYKDIHLDITAQPPRSYYTGLFIQCHFAENESRVLTGGYYKGSIEGFGLGLTL"
     gene            11960..12574
                     /locus_tag="POB55_001038"
     CDS             11960..12574
                     /locus_tag="POB55_001038"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HDI6500011.1"
                     /translation="MLRIAIAKGRLMDSLINYLDVIEYTTLSETLKNRERQLLLSVDN
                     IECILVKGSDVPIYVEQGMADIGIVGSDILDERQYNVNNLLNMPFGACHFAVAAKPET
                     TNYRKIATSYVHTAETYFKSKGIDVELIKLNGSVELACVVDMVDGIVDIVQTGTTLKA
                     NGLVEKQHISDINARLITNKAAYFKKSQLIEQFIRSLEVSIANA"
     gene            12567..13817
                     /locus_tag="POB55_001039"
     CDS             12567..13817
                     /locus_tag="POB55_001039"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HDI6500012.1"
                     /translation="MLNAQQFLNQFSLEAPLDESLYPIIRDICQEVKVHGDKALKMYN
                     LTFDHTKTDHLEISHEQIKAAFDTLDEKTKQALQQSYERIKAYQESIKQTNQQLEESV
                     ECYEIYHPLESVGIYVPGGKASYPSTVLMTATLAQVAGVENIVVVTPPQPNGVSQEVL
                     AACYITQVNQVFQVGGAQSIAALTYGTETIPKVDKIVGPGNQFVAYAKKYLFGQVGID
                     QIAGPTEIALIIDDTADLDAIVYDVFAQAEHDELARTYVIGEDAQVLKDLESRIAKAL
                     PNVDRYDIVSKSIANQHYLIHASNFDEACHVMNTIAPEHASIQTVNPQPYIEKVKYVG
                     ALFIGHYSPEVIGDYVAGPSHVLPTNRTARFTNGLSVNDFLTRNTVIHLSKDTFEQIA
                     DSAQHIAHVEALYNHQQSILIRQS"
     gene            13833..14846
                     /locus_tag="POB55_001040"
     CDS             13833..14846
                     /locus_tag="POB55_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate aminotransferase family
                     protein"
                     /protein_id="HDI6500013.1"
                     /translation="MIYIDKNESPVTPLDEKTMTSIISATPYNLYPDAAYEQFKEAYA
                     KFYGLLPEQIIAGNGSDELIQKLMLIMPKGPALTLNPDFFMYQAYAAQVNREIAFVDA
                     GSDLTFDLETILTKIDEVQPSFFIMSNPHNPSGKQFDTAFLTAIADKMKALNGYFVID
                     EAYLDYGMAYDVEMAPHILRMRTLSKAFGIAGLRLGVLISTAGTIQRIQKIEHPYPLN
                     VFTLNIATYIFRHREETRVFLTMQRQLAEQLKQIFDTHVADKMSVFPSNANFVLTKGS
                     AAQQLGQYVYEQGFKPRFYGEPVMKGYVRYSIATAPQLNQLEEIVKEWSAKYDLSKTT
                     KHS"
     gene            14815..15393
                     /gene="hisB"
                     /locus_tag="POB55_001041"
     CDS             14815..15393
                     /gene="hisB"
                     /locus_tag="POB55_001041"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HDI6500014.1"
                     /translation="MIYQKQRNTAETQLNISISDDQSPSHINTGVGFLNHMLTLFTFH
                     SGLSLNIEAQGDIDVDDHHVTEDIGIVIGQLLLEMIKDKKHFVRYGTMYIPMDETLAR
                     VVVDISGRPYLSFNASLSKEKVGTFDTELVEEFFRAVVINARLTTHIDLIRGGNTHHE
                     IEAIFKAFSRALGIALTATDDQRVPSSKGVIE"
     gene            15390..15968
                     /gene="hisH"
                     /locus_tag="POB55_001042"
     CDS             15390..15968
                     /gene="hisH"
                     /locus_tag="POB55_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HDI6500015.1"
                     /translation="MIVIVDYGLGNISNVKRAIEHLGYEVVVSNTSKIIDQAETIILP
                     GVGHFKDAMSEIKRLNLNAILAKNTDKKMIGICLGMQLMYEHSDEGDASGLGFIPGNI
                     SRIQTEYPVPHLGWNNLVSKHPMLNQDVYFVHSYQAPMSENVIAYAQYGADIPAIVQF
                     NNYIGIQFHPEKSGTYGLQILRQAIQGGFIND"
     gene            15961..16665
                     /locus_tag="POB55_001043"
     CDS             15961..16665
                     /locus_tag="POB55_001043"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-((5-
                     phosphoribosylamino)methylideneamino)imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HDI6500016.1"
                     /translation="MIELWPAIDLIGSTSVRLTEGKYDSEEKMSRSAEESIAYYSQFE
                     CVNRIHIVDLIGAKAQHAREFDYIKSLRRLTTKDIEVGGGIRTKSQIMDYFAAGINYC
                     IVGTKGIQDTDWLKEMAHTFPGRIYLSVDAYGEDIKVNGWEEDTELNLFSFVRRLSDI
                     PLGGIIYTDIAKDGKMSGPNFELTGQLVKATTIPVIASGGIRHQQDIQRLASLNVHAA
                     IIGKAAHQASFWEGLK"
     gene            16662..17420
                     /gene="hisF"
                     /locus_tag="POB55_001044"
     CDS             16662..17420
                     /gene="hisF"
                     /locus_tag="POB55_001044"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HDI6500017.1"
                     /translation="MIKKRIIPCLDVKDGRVVKGIQFKGLRDIGNPVDLAMYYNEAGA
                     DELVFLDISKTEEGHSLMLEVIEQTASRLFIPLTVGGGIQSLDDITQLLNHGADKVSL
                     NSSALKNPQLIKQASDKFGRQCICIAIDSYYDPERKAHYCCTTGGKKMTNIKVYDWVQ
                     QVEQLGAGELLVTSMGHDGMKQGFDIEHLANIKSLVNIPIIASGGGGNAQHFVELFDQ
                     TDVSAGLAASILHDRETTVQSIKEVIRQGGIAVR"
     gene            17417..18049
                     /locus_tag="POB55_001045"
     CDS             17417..18049
                     /locus_tag="POB55_001045"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HDI6500018.1"
                     /translation="MTNYKIDFSKGLVPAILQDNQTKQVLMLGYMNQEAFDKTIEDGV
                     VCFYSRSKQRLWTKGETSGHTQRVKDIHVDCDNDTILIDVIPNGPTCHTGSQSCFNTE
                     VPFSVQTLAQTVQDSAQSNNEKSYTKYLLTEGIEKITKKYGEEAFEVVIEAIKGDKKA
                     FVSEVADELYHLFVLMHALGVDFSEIEAELARRHHKRNNFKGERQNIEQW"
     gene            18242..18421
                     /locus_tag="POB55_001046"
     CDS             18242..18421
                     /locus_tag="POB55_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500019.1"
                     /translation="MKSELSNSGIDVTVKDVEKYMNRYNEVMKGKNGEKAKELCLSLL
                     PINIIVVFTFFVFIL"
BASE COUNT         6612 a         2791 c         3490 g         5981 t
ORIGIN      
        1 tataaatagg tcacttttaa tctgtatgta agcagactaa aagtggcctt ttaaacaaat
       61 aaaaaaaccg ttaacgtgtt gttaacggtt aataagctaa tatttatttt aaatttttta
      121 atatacgaga catttcttca attttaccag catgaagtgg gatatgaatg gcatttaata
      181 ctaataactc ttctaagtta ttacaaccaa caattggttg tttcaattca attgctaaat
      241 catcttcagt tagttttcgt gcacgttcag gtaatgtttg aagaccttct aaaatcgttc
      301 caatactcgg tacttcgccc ttccattcat ctggagacga accatttccg aataaaggtc
      361 tatatatatt taaatcaata ttctctttac cagtaacagc taaggctgat tcaaaaattg
      421 ttaatacatg cccaaattgc caatgaatgg tattagaaaa aggcttatga taatcatcaa
      481 gtatcttttc aactttccaa gtatcataca catttactaa atatcctaaa cccatatcaa
      541 tgacatcaaa taccgttttt gtagtcatta ttaattcctc cccccataaa taaaacatac
      601 ttccgtaagt attaccctat taacaaagtt gttagtcatt taaggaatgt attttattgg
      661 tgtacttata actattccgt tgtgcccagg gctaggtgta aatcctcatc attagaaata
      721 gcttcaatgg ctgcagtcaa acctgcaact atcttttcta atggcataga tggtgtatcg
      781 ggtttaccaa tgacctgctc tggtatgtat ggcacatgaa taaatccgaa tcgtaggtga
      841 gggtaatgct tatcttgtaa ataacctaag tgataaagtg tgtgattaca aacaaatgta
      901 cctgcgctat ttgaaagtgc tccaggaagt ccttgattaa taatactttg agtcattgct
      961 ttaactggta aatttgaaaa ataagctggc gcaccgtcta agtgaatggc ttgatcaata
     1021 ggttgaaaat catcattatc tggaatacgt gcatcatcaa tattaatggc gacacgttct
     1081 ggggtaatgg catttctacc accagcttgt cctatagcta gtacaacatc ataatgatta
     1141 gatgccaacg ttttatttat aatattatct actttcttaa aagaggttgg tagttttaat
     1201 ttatcgattg tatgtgtgcc aataatatct tctagttgag tcacagcttc ccatgaggga
     1261 ttgatatttt gattgtcaaa aggcgcaaac cctgttacta aaatgtgcat tttaagacac
     1321 cccgcagtat taaaatttaa tttagtataa cacgcgcata attaaaaaca tatcaggtta
     1381 tgtatgcgca taaaaaacag ccttgaaaaa cctttataaa tcggattttc aaagcgtgtt
     1441 ttgaaaggta tttaattaaa actaagcatg ttgatgtaaa tcatcaaagt ttgttaaacg
     1501 ttgttgccac tcattatcac taatattatt tgcttgaaca taacgattac gctcatgttt
     1561 agcacattca taagagcatg cacctaaata tttagtttcg ttttcttcag aaactaatat
     1621 ttgtttatta cattctgggt tagcgcaatt aatataacgt tcacatggtt tgccatcaaa
     1681 ccaatcctta ccaataattg ttttttcaac ttggttgata ttaacactga tgcggtcatc
     1741 aaatacgtac attttaccgt cccaatattc accttttgtt tcaggatctt taccatatgt
     1801 agcaataccg ccatgaagtt gtgctacatc ttcgaaacct tcttttaaaa gccatccaga
     1861 aaatttttcg catcgaattc caccagtaca gtacgtaacc acttttttat ctgcaaataa
     1921 cgctttattc tctttaatcc agtcaggcaa atctctaaaa cgtgtgatat ttggacgaat
     1981 tgcacctcgg aaatgaccta aatcaaattc ataatcatta cgtgcatcaa taatgactgt
     2041 gtcatcatct tcaagagctt ttctaaattc tacaggtgat aaatattggc cagttgtgtg
     2101 tcttggatcg acgtcatctt ctaaatctaa agcaacgatt tcttttcgag gacgtacatg
     2161 cattttctta aaagcatgtc cttcagcttc atcaatttta aacaccatat ctttgaatcg
     2221 ttcatcggca tgcatatgtg ccatatattg ttcggtttct tcttttgtac cagataatgt
     2281 gccgttaata ccttctgtag aaacaagaat tctacctttt aaatggtgtg ctttacaaaa
     2341 ggctaagtga tcctgagcaa actgttcagg gtcatcaatc gtcgtatatt tataatataa
     2401 aagaacttga tagttcatag gttaatctcc ttctaaaaac atgttataag catgttaatt
     2461 tttaaaataa agcgtcaata cgcatattta tctacgtcca tcattttaac agagttgtat
     2521 tgtcattcaa tatttctagc ttgaaatcag ccactagacc gataaaatag ataaatgaaa
     2581 ccgttatcat tttatttgat agttatcttg tttaaaatga aaatgatagt taaaacgaat
     2641 gaaatgaaag gaatgatcat gatgtcacaa caagatttac ctacattatt ttatagcggg
     2701 aagtccaata gtgctgttcc aattatatct gaaagtgaat tacaaacaat tacagctgaa
     2761 ccatggcttg aaatttccaa aaaaggattg cagctagaag gattgaactt tgatcggcag
     2821 ggacaactct ttttattgga tgtattcgaa ggcaatattt tcaaaatcaa tcctgaaacg
     2881 aaggaaatca aacgaccttt tgtaagccat aaagcgaatc ctgcagcaat caaaatacat
     2941 aaagatggcc gattattcgt ttgttattta ggagatttta aatctacagg aggcattttt
     3001 gcagctacag aaaatggtga caacatacaa gatattattg aagatttttc aacaacttat
     3061 tgtattgatg acatggtatt tgattctaaa ggtggatttt attttacaga ttttagagga
     3121 tactctacca atccactagg gggcgtttat tatgttgcgc cggactttag aacagtgacg
     3181 cctatcattc aaaatattag cgtagcaaat ggtattgctt taagtaaaga tgaaaaagta
     3241 ctatgggtaa cagaaactac agccaatcga ttacatcgca ttgcacttga agatgatggt
     3301 gtgacgatac aaccatttgg agctaccata ccgtactatt ttacaggtca tgaaggacca
     3361 gactcatgtt gtattgatag tgacgataat ttatatgtgg caatgtatgg tcaaggccga
     3421 gtattagttt ttaataaaag gggttatcca ataggacaaa tattgatacc aggccgagat
     3481 gaagggcata tgttacgttc tacgcatccg caatttatac ctgacacaaa tcaactcatc
     3541 atttgttcca atgatataga aatgggcgga ggatctatgc tttatacagt taatggcttt
     3601 gcaaaaggtc atcaaagttt tcagtttcaa taagacctac ctgaatgaaa ggcgtctaaa
     3661 tataagttta aatcttagcg atttttaagt tagaagttaa atataatttg cttcaatttg
     3721 taaaatgaag ttttgtataa cgtaaatagg caatatttaa tataagaaag tataaagaag
     3781 aattttcttg attatttcta atgaaatcaa tgccaattta ataaggagtt gtttattttg
     3841 gctatttata ttttttgtac aatactagtt ttagttgcag ttggagcact tttgacaaat
     3901 agaataaggg ataaaaaaga taaaaggcta gatattttat ctagtgtatt acttttaatt
     3961 tcatcaatat ctttgttact ttatggtgtt attatcaatt aagttaaata tgatttttaa
     4021 tttagaagtt aaaaaatgcc aactatacct agggatgata tagttggcat ttcgtatata
     4081 tatattaaaa caatgaggat tgtgtaatga gattattctg agattgagaa ttcagctgat
     4141 gcttcgaatt taacatcttt gcctagcatt acgccaccag tttcaagtgc ttggttaaag
     4201 ttaatgccgt agttttctct attgattgta ccagtaacaa taacacctgt tacttgagaa
     4261 ccatccattg gatttttact tactccgttg aattcaacat cgaatgtttc ttcgttagtg
     4321 atgcctttaa ttgttaaatc accaacaact ttgctttcag atactgattt tgtcacaaat
     4381 gtaattttat caaattcgtc agtaccaaag aaatcaccag attttaagtg gttatcacgt
     4441 gcttcgtttt tagtgttaat tgagcttgga ataattgttg cagtagcttt caaagtactg
     4501 aagtcattaa tatctccttc aacagctaca tcaaattgat caaatgaacc tttcacttta
     4561 gaaaccatta aatgtttaat ttggaattct aaactactgt gtgcaccatc aaaagtaaag
     4621 ttagtcataa tataaaacct ccatattttt atctcttaga tatatttttt atacttgata
     4681 tacatacaat accacctaaa aaacaaagat gcaagcattt tatttcgaat tcgaatattt
     4741 tctgtgatgt atttaaagaa tgacctataa aacaccacac tattgttata ccctagtcaa
     4801 ttaattcaaa cataaagaag tgattacaag tccgtgaaat ttagttaatg agataaatct
     4861 gactattatt atcattttta tagtcactac tattgacttt gcatcaagtg actcgtacta
     4921 ttttaatata attctgaaaa ttcaaaatac agaacattaa atatgtagat aatatttttt
     4981 tgaagtttta aaaaaaggag gcattttatg ggaaattatt tggtattgca atcagatttt
     5041 ggacttagtg atggcgctgt aagtgcaatg tatggagttg catatactgt aagtgatgat
     5101 atacgtgttg aaaacttaac gcatgatata ccaccgtatg acatttgggt agcgtcatat
     5161 cgtttatatc aaactgtaaa atactggcct aaaggcacag tttttgtatc agtggtagac
     5221 ccgggtgtag gtagtgatag gcggagcatt gcttgcttaa catattcagg tcattacatt
     5281 attacacctg ataatggttc tttaagtcat attaaacatt acgaaggtat taaaaaagtt
     5341 atcgaaattg atgaagtgaa aagccgattg cctcattcgg aagaaagtca tacttttcat
     5401 ggtagagatg tttatgcata caatggtgcg agattagctg caaataagat agcgtttgaa
     5461 cgtctcggtc aggcaattaa tgttgatagt attgaggcat tagaaataag agaagcgaca
     5521 aaaaatgagg attctgttac aggaagcatt gatattttag atatcagatt tgggtcatta
     5581 tggacaaata ttccattggc attcttaaaa tccttggaaa ttgttcatgg taataatttg
     5641 gtggtaacaa tttatcatca agataagaaa gtctatcaaa atattattaa atttgcgcga
     5701 tcgtttgcag atgttaatgt gggtgaacct cttgtgtatg ttaactcgct agtaaatatt
     5761 ggtgtagcgg tcaatcaaga ctcattttca gatttatatc atattggtac tggaaatgat
     5821 tggacgattc atttatctaa agcaccatca atttagtgaa ttttttatgt aaggagtaat
     5881 gaagatgaaa aaacaagata tttcagttaa aactgttgtt gcgattggta taggagcagc
     5941 ggtatttgtg attttagggc gttttgttgt aataccaaca ggttttccta atacgaatat
     6001 agaaacatct tatgcatttt tagcattaat atctgcaata tttgggcctt ttgctggttt
     6061 aatgactgga ttagttggac acgctattaa agatttcacg acatacggta gtgcttggtg
     6121 gagttgggtt atttgttcag gaattatagg ctgtttatat ggatggattg gcctaaaatt
     6181 aaatctttct tcaggccgat tttctaggaa atcgatgatt tattttaata tcggtcaaat
     6241 tattgcgaat attatttgct gggcacttat tgcaccaaca ttagatattt tgatttataa
     6301 cgaaccggct aacaaggttt atacacaagg tgttatctct gcagtattaa atattatttc
     6361 agttggcatt attgggacaa tattattaaa agcatatgct tcatctcaaa taaaaaaagg
     6421 tagtttacgt aaagaataat cattttgttg aatcagatat gtaaatgaat gtagaaaggt
     6481 aatgatatat catgactgaa ccaattatct cgttcaaaga ctttagtttt caatatcata
     6541 gtcaagcaac acctacatta cagaaaataa atgttgatat ttatccagga gaaaaagtat
     6601 tagtagttgg tgcttcgggt agtggtaaat cgacttttgc aaattgcata aacggattaa
     6661 ttccatttaa aactaaaggt aacataactg gggaactata tataaataat caagatgcaa
     6721 ccgttagttg tttacatgat agatctaatg ttgttggtac agttttacaa gatacagatg
     6781 gacagttcat aggcttaaca gcagctgaag atatggcctt tttattagaa aataattgtg
     6841 ttgaacaaga tgatatgaag aaaaatgtaa gttattgggc tgaaaaagtt ggcatgatag
     6901 aacatttaaa tcaccgaccg caagatttat ctggaggtca aaaacaacgc gtttcattag
     6961 gtggtatatt aatccatcgt acgcctattt taatattgga tgagccgctg gccaatttag
     7021 atcctgcgac aggacatgaa acgctgagat tgttaaacaa tattcatgaa gaaacaaagt
     7081 caacgatgat tattgtcgaa catcgattag aagaatcatt agatgatacg tttgatagag
     7141 ttcttctttt taaagatgga aaaatcatcg ctaatacaac gcctagtgat ttgctaaaat
     7201 catctaagct taaagaagca ggcatacgtg aaccattata ctgtacagcg cttaaatatg
     7261 cagaagtaga tgtcgaatcc attgataact tagcgaattt acgtgacgtt tgtatgagtg
     7321 aacatgttaa atttaaagtg aaaaaatgga tagatgaaac atctgcaaat aatgataata
     7381 agtacaaatc tgaaccgcta ttagaattaa atgaggtatg tgttcaatat agtgactata
     7441 gtaatagtgt acttaataat gttcaattaa atgtttatag aagagaaatg cttagtatag
     7501 ttggtcacaa tggtgccggc aaatcaacac ttgctaaagc aatttgtgga tttttagata
     7561 taacaggtaa tatccaattt tgtaataggg gatttaatca attgtcaatt agcgaacgat
     7621 ctgaattcgt aggttatgtg atgcaaaatc caaatcatat gatttccgaa aaaatgattt
     7681 acgatgaagt agcattaggg ttaagagcac gaggtatgaa agaatcagat ataaagatac
     7741 gggtagagaa tgtgctcaaa atatgcggac tttatgcatt tcggaattgg ccaattgtag
     7801 ctttaagcta tggtcaaaaa aagagggtca ctatagcatc tgttttagtc ttaaatccgg
     7861 aaataatcat attggatgaa ccgactgctg gtcaagattt ctatcattat aatgagataa
     7921 tgtcattttt aattgagcta aacagacaag ggaagacgat tattatgatt acgcatgata
     7981 tgcatttatt gtctgagtat agttcaagaa cagttgtatt atcaaaaggt caagtcgttg
     8041 ctgataccac gccagtattg gttttaaatg ataaaaaaat ctgtgagatt gcatcattga
     8101 gacaaacatc gctatttgaa atggccgaat atatagggat tagcgagcca cagaaattag
     8161 tacaattatt tattaaccat gataggaagg tgagacgcca atgaatcaat ataatactat
     8221 aggttttcac ccgggaaata gtcgtattca tcaattaaat gcgactgtta aacttttatt
     8281 cttattagtt gtttctattt ctgcaatggt gacttatgac acaagatatt taattttaat
     8341 tagtgtttca tctattttat tgttcaaata tgctcatatt gaatggaaac aagttcgttt
     8401 tgttgttaaa ttcattctgt ttttcacaat attaaatatt attgccgtat acatatttga
     8461 tcctgaatat ggtgtgaaga tttataatca gcgtacagag ttaatcaatg gtattggtcg
     8521 atttacgcta acatcacagg aattattcta tctttttaat ctaatattaa aatatattag
     8581 tacagttcct ttagcgttaa tatttttatt cacaacgaat ccgagtcatt ttgctgcaag
     8641 tttaaatcag ctaggtgtga attataaaat cagttacgca gtctcactag cattaaggta
     8701 tattccagat attcaagaaa catattttaa tatttcacaa gcacaacaag caagaggata
     8761 tgataattcg aaaaaggcga aatttactag ccgtgttaaa ggtatcaaac gcattgtgtt
     8821 acctttaata ttttctagta tcgaaagaat tgacactatt agtactgcta tggagttaag
     8881 acaattcgga cagtataaaa ggagaacctg gtacgtcaaa aaacaattaa aaaaagatga
     8941 ttatgttgtt ttgtgtttga cgttaatact tctgatgtta gtagttacat tattcttttt
     9001 aaataatagt cgatatttca acccgtggta ttagcattca tataaatagt ctttaaatag
     9061 aaataggagg gagacattta atgataaata ctgaaagatt aaatttaatg attccaagtt
     9121 cctcgcattt aattgaactt tataatattt gtagtcatcc acaagcaaat atatacactc
     9181 ccaaaggttt acataattcc aaattagaca cacaacggtg gattgaaaaa tggcgaaacc
     9241 attggcaaca atatcaattt ggttactttg tattggtaaa aaaaatagat tgtagtgtta
     9301 ttggtatttg tggatatgaa tatcgacaat taaagcaaga aacagtatta aatttatttt
     9361 ataaattaca tccaagtttt gaaggacaag ggtacgcatg tgaggctatt acagcaatca
     9421 caaattttgt gaattatatc gatcaagaaa cagtaaaagt tatcaggaca aataagtgta
     9481 accaacgttc aataaattta gcagaaaggc ttaaattcaa gcgagacaaa gctatggacg
     9541 acattatcaa tcaaggagat attgtgtttt ataaataaaa tactatgaca ttatctaaaa
     9601 aataaaatta aaatcccaac agtttcttaa aaagttatga gccacataat aaaaatgtta
     9661 taattgtgca taataaaaca tagggagaat gtttaatgca taaaaaatat ttattgctat
     9721 tttttctgtt attatcagtt gcattgctat atagctgttc caagcaaaat gagaaagctc
     9781 aaaataaatc tgttaatcca aatgagtatg caaagcataa atcgaaaatg gatgaacatt
     9841 gggaaacata tcacggccaa gtttatcatg tgttttatca cccattaatt acagacccaa
     9901 aagttgcgtt tacaggagat acacaccaag ctaagggcaa caatgattgg atgattactg
     9961 taagtgaatt taaaaaatca ttgaacgaat tatataaaaa taattacatc atcgttaatc
    10021 cacatgatgt atacgactta tcatctcatc atgtaaaagc caaaaaaata aaattgccga
    10081 aaggtaaaaa gccactaatc atctcaattg atgatatgaa ttactatagt tacatgagac
    10141 accatggtta tgctgaccgt ttagtactcg ataaaaataa acatgttgtt tctgaaacta
    10201 cagacaaaaa aggtcatgct acttattctg atgacaatga tatcgtgcca attttaaata
    10261 aatttgtaaa agagcatcca gatttttctt taaatggtga aaaaggtgtt gttgctttaa
    10321 ccggttatga gggcgtatta ggttatcgca cgaatgaatt aacgagtaaa gattatacaa
    10381 agaataaaaa ggcagctgag gaagttgttc gcgcaatgaa acgggatgga tggtcatttg
    10441 cgagtcattc atatggtcac attaattttg aaaaaacatc tttagaaggt attaaacgtg
    10501 atactaaaag atggaaagat gaagttgaac caattgtagg taaaacagat atgtttgtat
    10561 ttccacatgg cgcacaggat agacatacac aagcgtatga ttatttggta gatgaagcag
    10621 agtttaagtt tattgcaggt gtcggtccaa acaattttac agatatatca gcaacgaacg
    10681 tttaccaaga tagggttgcg attgatggtc taaacttatt tgaatttaaa tataaactca
    10741 aaccgttttt taatcctgaa aatgtatata gtaaacaaga tagacaattc tttaaaggta
    10801 atcgggatta tgaagaatag ttaaatttaa taaaaagata gaaagaaaat aactttacat
    10861 aataaggcaa gcacaatatg tgcaagaagt acaacttcaa tataaaaatc ttggtttagc
    10921 atttttatag gcacaaatgt cttgcctttt tcttttgtaa aaatttaaaa acaatacacc
    10981 gaattttctg aaaaaggttg taatgtattg ctaaaaatga taagttatta atcatattac
    11041 actttagtgt ggtagagaag taaagtaaac aacgaaaata ccaagtgatt ttaaatacag
    11101 tattgaaagt aaagggtggt cgagtgaata attcagaaca attaattgcg ttaaaagaat
    11161 cggaaacagc atttttaaaa tattttaata aagcggatta tgagttagtt gattttagtg
    11221 tggttgaaaa gttggactgg aaacaattga accatgagga tttgcagcag atgggtgagc
    11281 gaaatttttg gcaacatgag catcaaattt atgcacttcg aaatgatttt acagatcaat
    11341 tattgcgtta ttactcaatg tatccaactg cagcaacaaa agtggcatac acaggtttaa
    11401 tcattcggaa taatgaagcg gctgtacaag tgggtttgga aaactatgca ccatcattag
    11461 cgaatgtaca acagagctta aagctattta ttcagtttat tcaacagcag ttgagagaca
    11521 acgtgcattt tgttgtattg ggacattatc aattgcttga tgccttactc gataaatcgt
    11581 tacaaacacc agacatacta tcaatgattg aggaacgtaa tttatcggga ctggttacat
    11641 atttgtcgac tgaacatcca atcgtgcaaa tcttgaaaga gaatacgcaa caacaattaa
    11701 atgtattgga acactatatt ccaaatgacc atccagcact cgttgaatta aaaatatggg
    11761 aacgttggtt acatacacaa ggttacaaag acatccattt agatattact gcgcagccac
    11821 ctagatccta ttacacaggc ttatttattc aatgtcattt tgctgaaaat gaatctcgag
    11881 ttttaacagg tggttattac aaaggaagca tcgaagggtt tggattagga ttaacacttt
    11941 aagtaaggga gtatgcacaa tgttaagaat cgccatagcc aaaggacgtc taatggatag
    12001 tttaattaac tatttagatg taattgaata tacgacatta tcagaaacat taaaaaatag
    12061 agaacgccaa ttattattaa gtgtagataa tattgaatgc attttagtaa aaggaagtga
    12121 cgtgccaatc tatgtggaac aaggaatggc cgacataggc attgttggta gcgacatatt
    12181 agatgagcgc caatataatg ttaataattt gttgaatatg ccttttggag catgtcattt
    12241 tgcggttgca gcgaaacctg aaacgaccaa ttatcgtaaa atcgcaacga gttatgttca
    12301 tactgctgaa acatatttta aatcaaaagg tattgatgtc gaattgatta aattgaatgg
    12361 ttctgttgaa ttagcttgtg tcgtagatat ggtagacgga attgtcgaca tcgttcaaac
    12421 aggtactacg ctaaaagcga acggactggt tgaaaagcaa catattagtg atatcaatgc
    12481 aagattaata actaataaag cagcttattt taaaaaatca caattaatag agcaatttat
    12541 tcgctctttg gaggtgtcta ttgccaatgc ttaatgcaca acaattttta aatcaatttt
    12601 cattagaagc accattagat gagtcattgt atccaattat tcgcgatatt tgtcaggaag
    12661 ttaaagttca tggagataag gctttgaaaa tgtataatct aacattcgat catacgaaaa
    12721 cagatcattt agaaattagt catgaacaaa ttaaagcagc atttgacaca ttagatgaaa
    12781 aaacaaaaca agcattacaa caaagttatg aaagaattaa agcatatcaa gaaagtatta
    12841 agcagacgaa tcaacagtta gaagaatcag tggagtgtta tgaaatatac catccactag
    12901 aaagtgtggg tatttatgtg cctggtggca aagcaagtta tccatcaacg gttctaatga
    12961 cagcgacttt agcacaagta gcgggtgtag aaaatattgt tgttgtgaca ccacctcaac
    13021 ctaacggagt atcccaagag gtattagctg catgttatat tacgcaagtt aatcaagtgt
    13081 ttcaagttgg tggtgctcaa agtattgctg cattgactta tggaacagaa acgataccta
    13141 aagttgataa gattgtaggt ccaggtaacc aatttgttgc atatgccaaa aaatatttat
    13201 ttggacaggt aggtattgat caaattgcag gaccaacaga aatagcactg attattgacg
    13261 acaccgcaga tttagatgcc atcgtatatg atgtatttgc gcaagcagaa catgatgaat
    13321 tagcacgtac atatgtcatt ggtgaagatg cgcaagtcct taaagattta gaatcacgta
    13381 ttgctaaagc attgcctaat gtggacagat acgacattgt ttctaaaagt atcgctaatc
    13441 aacactacct tatccatgct agtaattttg atgaagcatg ccatgtcatg aatacaatcg
    13501 cgcctgaaca tgcgtcgatt caaacagtaa atcctcaacc atatattgaa aaagtgaaat
    13561 atgtgggtgc attgtttatt ggacattatt cgccagaggt cataggagat tacgttgcag
    13621 gtccaagtca tgtattacct acaaatagaa cagctagatt taccaatggg ttatcggtca
    13681 atgatttctt aacacggaac acggtcatcc atttatcaaa agatacgttt gaacaaattg
    13741 ctgattcagc acaacatatt gctcatgttg aagcattata caatcaccag cagtctattt
    13801 taatacgtca gtcttagggg agtgtaattg aaatgattta tattgataaa aatgaaagtc
    13861 cagttacgcc gttggatgaa aaaacaatga cgtctattat tagtgcaacg ccatataatt
    13921 tatatcctga tgcagcatat gaacaattca aggaagctta tgctaagttt tacggattat
    13981 tgcctgaaca aattattgca ggaaatggct ctgatgaatt gattcaaaag ttaatgctaa
    14041 tcatgccaaa aggtccggca ttaacgctaa atcctgattt ttttatgtat caagcatatg
    14101 cggcacaagt aaatcgtgaa attgcatttg tagatgcagg atcagattta acgtttgatt
    14161 tggaaaccat tttaacgaaa atcgatgaag tacaaccatc attttttatt atgagtaatc
    14221 cacataaccc ttcaggcaag caatttgata cggcattttt aacagctatt gcagataaga
    14281 tgaaagcatt aaacggatac ttcgtcattg atgaagcata tttagattat ggcatggcat
    14341 atgacgttga aatggcacca cacattttaa gaatgcgcac attatcaaag gcgtttggaa
    14401 tagctggctt aagattaggt gtcttaatta gtactgctgg aacgatacag cgtattcaaa
    14461 aaatagaaca tccatatcca ttaaatgtat ttacgctaaa tattgcgact tatattttta
    14521 gacatagaga agagacaaga gtatttttaa cgatgcaacg acagttagct gagcagttaa
    14581 aacaaatatt tgatacacat gttgcagata aaatgtcagt attcccatca aatgccaatt
    14641 ttgtacttac taaaggttca gcagcgcaac aattaggaca atacgtatat gaacaaggat
    14701 ttaaacctcg cttttatggt gagccggtaa tgaagggcta tgtaagatac tcaattgcaa
    14761 cagcaccaca gttaaaccaa ttagaagaaa ttgttaaaga atggagcgca aaatatgatt
    14821 tatcaaaaac aacgaaacac agctgaaaca caactaaata tttcaatatc agatgaccag
    14881 tcaccatcgc atattaatac aggtgtgggc tttttaaatc atatgttaac attgtttaca
    14941 tttcatagcg gtctgtcatt aaacattgag gcacaaggtg atattgacgt agatgatcac
    15001 cacgtaactg aagatatcgg cattgtcatt ggccaattgt tacttgaaat gattaaagat
    15061 aaaaagcatt tcgttcgtta tggaacgatg tacattccaa tggatgaaac attagcacgt
    15121 gtcgttgtgg atataagtgg gcgcccatac ctatcattca atgcatcatt aagtaaagaa
    15181 aaagttggta cgtttgatac ggagttagta gaagaatttt ttagagcggt cgtaatcaat
    15241 gcaagattaa caacgcatat tgatttaatt cgtggaggta atacacacca tgaaattgaa
    15301 gctatattca aagcgttttc ccgtgcatta ggcatagcgc taactgcaac tgatgatcag
    15361 cgtgtgccgt catcgaaagg tgtgattgaa tgattgtcat cgttgattat ggattaggga
    15421 atattagtaa tgtaaaacgc gctattgaac atttagggta tgaggtggtt gtctcaaata
    15481 cctcaaaaat aatcgatcaa gcagaaacaa tcatattgcc cggtgtcggc cattttaaag
    15541 atgcgatgtc agagataaag cgattaaatc tcaatgcaat attggctaag aatactgata
    15601 agaagatgat tggtatttgt ttaggcatgc aattaatgta tgagcatagt gatgaaggcg
    15661 atgcatctgg attagggttt atcccaggaa atatttcgcg tatccaaaca gaatacccag
    15721 tgccacactt aggctggaat aatttagtga gtaagcaccc tatgttaaat caagatgttt
    15781 atttcgtaca ttcttatcaa gcgccgatgt cagaaaatgt aattgcatat gcgcagtatg
    15841 gggctgatat tccggcaatt gttcaattta acaattatat tggtattcaa ttccatcctg
    15901 aaaaaagcgg tacatatggg ttacaaattt tgcgtcaggc aatacaaggg ggatttataa
    15961 atgattgaat tatggccagc gattgatttg attgggtcaa caagtgtgag gttaacagag
    16021 ggtaaatatg atagtgaaga aaaaatgtca cgctcggctg aagaaagtat tgcttactat
    16081 agtcaatttg aatgtgtgaa tcgtattcat atcgtcgact tgataggtgc taaggcacag
    16141 catgcccgag agtttgatta tattaagtca ttgaggagat taacaaccaa agatattgaa
    16201 gtaggtggtg gcattcgtac gaagtcacaa atcatggact actttgccgc agggattaat
    16261 tattgcatag ttggaacgaa aggtattcaa gatactgatt ggcttaaaga gatggcacat
    16321 acatttccag gtcgcattta tttatctgtt gatgcctatg gagaagatat taaagtgaac
    16381 ggatgggaag aggacacaga gttaaattta tttagttttg tgagacggtt atcggatata
    16441 cctcttggcg gcattatata tactgatatt gctaaagatg gcaaaatgtc cggacctaac
    16501 tttgaattaa ctggtcaatt agtaaaggca acgacgattc ctgtcattgc ttccggtggt
    16561 attagacatc agcaagatat tcaacgatta gcgtcgctaa atgttcacgc tgctattata
    16621 ggaaaggctg cacatcaagc atctttttgg gaggggctaa aatgattaaa aaacgtatca
    16681 ttccatgttt agatgtcaaa gatggtcgtg tcgttaaagg gattcaattt aaaggattaa
    16741 gggatattgg gaatcctgtt gatttagcaa tgtattacaa tgaagcgggt gctgatgaat
    16801 tagtattttt agacatctct aagacggaag agggtcatag cttaatgcta gaagtgattg
    16861 aacagacagc gtcacgcttg tttatccctc ttactgtagg gggtgggatt caaagtctcg
    16921 atgatattac ccaattgcta aatcatggtg cagataaagt atcattaaat tcaagtgctt
    16981 taaaaaatcc acagctcatt aaacaagcga gtgataaatt cggtagacaa tgcatctgca
    17041 tagcaattga tagctattat gatcctgaaa gaaaagcaca ttattgttgt acgactggtg
    17101 gtaaaaaaat gacaaatatt aaagtatatg actgggtaca gcaagtagaa cagttaggtg
    17161 caggtgagct cctcgttaca agtatgggac atgatggtat gaaacaaggc tttgatattg
    17221 aacacctagc aaatattaag tctcttgtaa atattccaat cattgcttct ggtggtggtg
    17281 gcaatgcaca acactttgta gaattatttg atcagacgga tgtttctgca ggtttagctg
    17341 caagtatatt acatgatcga gaaacgacgg ttcaatctat taaagaagtg atacggcaag
    17401 gaggtatagc agtaagatga ccaattacaa aattgatttt agcaaaggtt tagtgccagc
    17461 aattttacaa gataatcaaa caaaacaagt attgatgttg ggttatatga atcaagaagc
    17521 ttttgataaa acgatagaag atggtgtagt atgtttctat tcgcggtcga aacaacgtct
    17581 atggacaaaa ggtgaaacat ctggtcatac gcaacgtgtt aaagatattc atgtagattg
    17641 cgacaatgat actattttaa ttgatgtcat accaaatgga ccaacatgtc atacaggcag
    17701 tcaaagttgt ttcaacacag aagttccatt ttcagtgcaa acattagcgc agacagttca
    17761 agatagtgcc caatccaata atgaaaagtc atatacaaaa tatttattaa cagaaggtat
    17821 agaaaagatt acgaaaaaat atggtgaaga agcttttgaa gtcgtaattg aagcaattaa
    17881 aggtgacaaa aaagcatttg taagtgaagt agcagatgaa ctttatcatt tatttgtctt
    17941 gatgcatgcg ctaggcgtcg atttttcaga aattgaggca gaattagcgc gtagacatca
    18001 taagcgcaat aactttaaag gcgaacgaca aaatatcgaa cagtggtaaa acaagtatgg
    18061 gctgagatat agagaaacgg atcatggctt atacacttac aaatattgtg gaaaacgtga
    18121 cattttcaag tttaaaatac gacaccaaca tattttaact atgaatgctg tgatggtact
    18181 aaagttgcaa acgcgttata gataagtagt ggataatcac aatacgaaac caaaaataat
    18241 tatgaaaagt gaattgagca actcaggaat agatgtcact gttaaagatg ttgaaaagta
    18301 tatgaatcga tataatgaag ttatgaaggg aaaaaatggc gaaaaagcta aagagttatg
    18361 tttgtcgtta ctacctatta atatcatagt tgtctttaca ttctttgtat ttatactata
    18421 aataaaaata tatctagcct gaaatagaaa tgtcatagcc tatttaaaag acaatctcca
    18481 ttagaactaa gatgtgcatc ccgaaagtta gactaaaaaa ctaactttat gggatgtatt
    18541 tttatgctaa tcatcataaa ttcgagatta agttaaaggt agttcaagag taattaaaca
    18601 ataaattaaa aatagtagga tacttacttt gagggaagaa aattaactgt atatatttag
    18661 tttaggaaca agtattacgg tttatcctga tacaattatt gtggatggga tgatattttt
    18721 aggtttaaaa tacgacacca gcaaacataa taactgtaat agctcataaa tctccccata
    18781 tagctaatct aaaaaaataa tacatcattg gaattaagcc ccaagcatgt aaatattaaa
    18841 aatcaaaaaa gatatatgta aaaaagttac aatt
//
LOCUS       DAMZKC010000041        17842 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.041, whole genome shotgun sequence.
ACCESSION   DAMZKC010000041 DAMZKC010000000
VERSION     DAMZKC010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 17842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17842
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.041"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(<1..227)
                     /gene="nrdH"
                     /locus_tag="POB55_001047"
     CDS             complement(<1..227)
                     /gene="nrdH"
                     /locus_tag="POB55_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HDI6500020.1"
                     /translation="MSEIIVYTQNDCPPCTFVKNYLNEHQIDFEERNINNQQYRNEMI
                     DFDAFSTPFILLNGNPMYHVDLDEINKVLNI"
     gene            425..1786
                     /locus_tag="POB55_001048"
     CDS             425..1786
                     /locus_tag="POB55_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HDI6500021.1"
                     /translation="MDTVEISRFLTAMTLAVHIIFATIGVGMPLMFAIAEFLGIRKND
                     LQYIAMAKRWAKAYTITVAVGVVTGTIIGLQLSLIWPTFMEMGGHVIALPLFMETFAF
                     FFEAIFLSIYLYTWDRFKNKWTHFLISIPVIIGGSFSAFFITSVNSFMNTPAGFELKN
                     GKMVNVQPIEAMFNPSFIVRSFHVITTAGMTMAFVIASIAAFKLLRNRQPKDTVYHKK
                     ALKMSMIVGFFSTLLSMLAGDLSAKFLHKFQPEKLAAYEWHFDTSSHAKLLLFGVLDE
                     KTQQVKGAIELPGLLSFLADNSVKTKVQGLNDFPKSLHPPMIVHYFFDLMVTMGILCF
                     VISGVYVLTLMFKKLRKFSTHKWMLYGILLTGPASMLAIEFGWFLTEMGRQPWIVRGY
                     MRVAEAATQAGGITFVTILFGILYIILMYTCAYVLIRMFKNKPAYEDVNRLAKKQGGE
                     IEK"
     gene            1783..2802
                     /locus_tag="POB55_001049"
     CDS             1783..2802
                     /locus_tag="POB55_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HDI6500022.1"
                     /translation="MIYAFIGITVLWLFLFCYIIIASIDFGAGFFALHSKLTGDEKKI
                     NHLISRYLNPVWEVTNVFFVFFFVGFVGFFPESIKYLGTVLLIPGSIALIMISLRNSF
                     YAFENYGQDTKLAWMIMYGVSGLLIPASLSTALTITEGGYINVRNNVIDLDWVQLLLS
                     PFAWSVVFLAIISVLYISSGFLTYYAKKANDEPAYNLTRQWHIFLGPPMIIICLFVFL
                     SLRIQNSEHFYSAVFDYWWMFAISFLFFALASLLTFFKKKHGLAFVFVILQMMFAFFG
                     YGISKLPYLLYPFVKITDAYVNPEMGWTLVIVFILGLLLLLPSLILLLRLFVFDKEYV
                     EGKKS"
     gene            2935..3597
                     /locus_tag="POB55_001050"
     CDS             2935..3597
                     /locus_tag="POB55_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HDI6500023.1"
                     /translation="MGKEYVVIGLGRFGGSIVRELNALDMDVMAIDHDENRVNEYSDI
                     ATHAVVADTTDEAVMKSLGIRNFDHVIVAIGENIQSSTLTTLILKELGVKKVTAKAQN
                     DYHAKILNKIGADTVVHPERDMGRRIAHNVASASVLDYLELADEHSIVELKATEKMAG
                     QSIIDLDIRAQYGINIIAIKRGKEFIISPNPNINLEIGDILIMIGHDNDLNRFEKNIA
                     TR"
     gene            complement(3946..5643)
                     /locus_tag="POB55_001051"
     CDS             complement(3946..5643)
                     /locus_tag="POB55_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDI6500024.1"
                     /translation="MKQLHPNEVGVYALGGLGEIGKNTYAVEYKDEIVIIDAGIKFPD
                     DNLLGIDYVIPDYTYLVQNQDKIVGLFITHGHEDHIGGVPFLLKQLNIPIYGGPLALG
                     LIRNKLEEHHLLRTAKLNEINEDSVIKSKHFTISFYLTTHSIPETYGVIVDTPEGKVV
                     HTGDFKFDFTPVGKPANIAKMAQLGEEGVLCLLSDSTNSLVPDFTLSEREVGQNVDKI
                     FRNCKGRIIFATFASNIYRVQQAVEAAIKNNRKIVTFGRSMENNIKIGMELGYIKAPP
                     ETFIEPNKINTVPKHELLILCTGSQGEPMAALSRIANGTHKQIKIIPEDTVVFSSSPI
                     PGNTKSINRTINSLYKAGADVIHSKISNIHTSGHGSQGDQQLMLRLIKPKYFLPIHGE
                     YRMLKAHGETGVECGVEEDNVFIFDIGDVLALTHDSARKAGRIPSGNVLVDGSGIGDI
                     GNVVIRDRKLLSEEGLVIVVVSIDFNTNKLLSGPDIISRGFVYMRESGQLIYDAQRKI
                     KTDVISKLNQNKDIQWHQIKSSIIETLQPYLFEKTARKPMILPVIMKVNEQKESNNK"
     gene            complement(5643..5861)
                     /locus_tag="POB55_001052"
     CDS             complement(5643..5861)
                     /locus_tag="POB55_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HDI6500025.1"
                     /translation="MAVFKVFYQHNRDEVIVRENTQSLYVEAQTEEQVRRYLKDRNFN
                     IEFITKLEGAHLDYEKENSEHFNVEIAK"
     gene            complement(5890..6012)
                     /locus_tag="POB55_001053"
     CDS             complement(5890..6012)
                     /locus_tag="POB55_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500026.1"
                     /translation="MNDMMNLVINQIVIKLHYRDDWNEFIDIYSNTILLGLTIN"
     gene            complement(6343..6894)
                     /locus_tag="POB55_001054"
     CDS             complement(6343..6894)
                     /locus_tag="POB55_001054"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDI6500027.1"
                     /translation="MLTMKDIIRDGHPTLRQKAAELELPLTKEEKETLIAMREFLVNS
                     QDEEIAKRYGLRSGVGLAAPQINISKRMIAVLIPDDGSGKSYDYMLVNPKIVSHSVQE
                     AYLPTGEGCLSVDDNVAGLVHRHNRITIKAKDIEGNDIQLRLKGYPAIVFQHEIDHLN
                     GVMFYDHIDKDHPLQPHTDAVEV"
     gene            7259..7885
                     /locus_tag="POB55_001055"
     CDS             7259..7885
                     /locus_tag="POB55_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkyA family protein"
                     /protein_id="HDI6500028.1"
                     /translation="MKFGKTIAVVLASSVLLAGCTTDKKEIKAYLKQVDKIKDDEEPI
                     KTVGKKIAELDEKKKKLTEDVNSKDTAVRGKAVKDLIKNADDRLKEFEKEEDAIKKSE
                     QDFKKAKSHVDNIDNDVKRKEVKQLDDVLKEKYKLHSDYAKAYKKAVNSEKTLFKYLN
                     QNDATQQGVNEKSKAIEQNYKKLKEVSDKYTKVLNKVGKEKQDVDQFK"
     gene            8056..9168
                     /gene="pdhA"
                     /locus_tag="POB55_001056"
     CDS             8056..9168
                     /gene="pdhA"
                     /locus_tag="POB55_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002467471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HDI6500029.1"
                     /translation="MAPKLQAQFDAVKVLNDTQSKFEMVQILDENGNVVNEDLVPDLT
                     DEQLVELMERMVWTRILDQRSISLNRQGRLGFYAPTAGQEASQLASQYALEKEDYILP
                     GYRDVPQIIWHGLPLTEAFLFSRGHFKGNQFPEGVNALSPQIIIGAQYIQAAGVAFAL
                     KKRGKNAVAITYTGDGGSSQGDFYEGINFAAAYKAPAIFVIQNNNYAISTPRSKQTAA
                     ETLAQKAIAVGIPGIQVDGMDALAVYQATKEARDRAVAGEGPTLIETMTYRYGPHTMA
                     GDDPTRYRTSDEDAEWEKKDPLVRFRKFLENKGLWNEDKENEVIERAKADIKAAIKEA
                     DNTEKQTVTSLMEIMYEDMPQNLAEQYEIYKEKESK"
     gene            9172..10149
                     /locus_tag="POB55_001057"
     CDS             9172..10149
                     /locus_tag="POB55_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017637695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HDI6500030.1"
                     /translation="MAQMTMVQAINDALKTELKNDQDVLIFGEDVGVNGGVFRVTEGL
                     QKEFGEDRVFDTPLAESGIGGLAMGLAVEGFRPVMEVQFLGFVFEVFDAIAGQIARTR
                     FRSGGTKTAPVTIRSPFGGGVHTPELHADNLEGILAQSPGLKVVIPSGPYDAKGLLIS
                     SIRSNDPVVYLEHMKLYRSFREEVPEEEYTIDIGKANVKKEGNDISIITYGAMVQESM
                     KAAEELEKDGYSVEVIDLRTVQPIDVDTIVASVEKTGRAVVVQEAQRQAGVGAAVVAE
                     LSERAILSLEAPIGRVAAADTIYPFTQAENVWLPNKNDIIEKAKETLEF"
     gene            10240..11532
                     /locus_tag="POB55_001058"
     CDS             10240..11532
                     /locus_tag="POB55_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HDI6500031.1"
                     /translation="MAFEFRLPDIGEGIHEGEIVKWFVKAGDTIEEDDVLAEVQNDKS
                     VVEIPSPVSGTVEEVMVEEGTVAVVGDVIVKIDAPDAEDMQFKGHDDDSSSKEEPAKE
                     EAPAEQAPVATQTEEVDENRTVKAMPSVRKYAREKGVNIKAVSGSGKNGRITKEDVDA
                     YLNGGAPTASNESAASATSEEVAETPAAPAAVTLEGDFPETTEKIPAMRRAIAKAMVN
                     SKHTAPHVTLMDEIDVQALWDHRKKFKEIAAEQGTKLTFLPYVVKALVSALKKYPALN
                     TSFNEEAGEIVHKHYWNIGIAADTDRGLLVPVVKHADRKSIFQISDEINELAVKARDG
                     KLTADEMKGATCTISNIGSAGGQWFTPVINHPEVAILGIGRIAQKPIVKDGEIVAAPV
                     LALSLSFDHRQIDGATGQNAMNHIKRLLNNPELLLMEG"
     gene            11536..12942
                     /gene="lpdA"
                     /locus_tag="POB55_001059"
     CDS             11536..12942
                     /gene="lpdA"
                     /locus_tag="POB55_001059"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009383646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDI6500032.1"
                     /translation="MVVGDFPIETDTIVIGAGPGGYVAAIRAAQLGQKVTIVEKGNLG
                     GVCLNVGCIPSKALLHASHRFVEAQHSENLGVIAESVSLNFQKVQEFKSSVVNKLTGG
                     VEGLLKGNKVNIVKGEAYFVDNNSLRVMDEKSAQTYNFKNAIIATGSRPIEIPNFKFG
                     KRVIDSTGALNLQEVPGKLVVVGGGYIGSELGTAFANFGSEVTILEGAKDILGGFEKQ
                     MTQPVKKGMKEKGVEIVTEAMAKSAEETDNGVKVTYEAKGEEKTIEADYVLVTVGRRP
                     NTDELGLEELGVKFADRGLLEVDKQSRTSISNIYAIGDIVPGLPLAHKASYEAKVAAE
                     AIDGQAAEVDYIGMPAVCFTEPELATVGYSEAQAKEEGLAIKASKFPYAANGRALSLD
                     DTNGFVKLITLKEDDTLIGAQVVGTGASDIISELGLAIEAGMNAEDIALTIHAHPTLG
                     EMTMEAAEKAIGYPIHTM"
     gene            13110..13385
                     /locus_tag="POB55_001060"
     CDS             13110..13385
                     /locus_tag="POB55_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HDI6500033.1"
                     /translation="MEYEYPIDLDWSNEEMISVINFFNHVEKYYESGVTAGDFMGAYK
                     RFKEIVPAKAEEKQIFNTFEKSSGYNSYKAVQDVKTHSEEQRVTAKK"
     gene            13529..14068
                     /locus_tag="POB55_001061"
     CDS             13529..14068
                     /locus_tag="POB55_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI6500034.1"
                     /translation="MNIGNKIKNLRRIKNLTQEELAERTDLSKGYISQIESEHASPSM
                     ETFLNIIEVLGTTPSEFFKDSENEKVLYKKEEQVIYDEYDEGYILNWLVSKSNEYDME
                     PLILTLKPGASYKNFNPSESDTFIYCMSGQITLNLGKEIYQAQEEDVLYFKARDNHRL
                     SNESNNETRILIVATASYL"
     gene            14081..15175
                     /locus_tag="POB55_001062"
     CDS             14081..15175
                     /locus_tag="POB55_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6500035.1"
                     /translation="MEPLLSLKSVSKSYDDLNILDDIDIDIESGYFYTLLGPSGCGKT
                     TILKLIAGFEYPDSGEVIYQNKPIGNLPPNKRKVNTVFQDYALFPHLNVYDNIAFGLK
                     LKKLSKTEIDQKVTEALKLVKLSGYEKRNINEMSGGQKQRVAIARAIVNEPEILLLDE
                     SLSALDLKLRTEMQYELRELQSRLGITFIFVTHDQEEALALSDFLFVLKDGKIQQFGT
                     PTDIYDEPVNRFVADFIGESNIVEGRMVRDYVVNIYGQDFECVDMGIPENKKVEVVIR
                     PEDISLIKAEEGLFKATVDSMLFRGVHYEICCIDNKGYEWVIQTTKKAEVGSEVGLYF
                     DPEAIHIMVPGETEEEFDKRIESYEEVDNA"
     gene            15168..15965
                     /locus_tag="POB55_001063"
     CDS             15168..15965
                     /locus_tag="POB55_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6500036.1"
                     /translation="MRNTNKFLLIPYLLWMVIFIIVPVVLLIYFSFLDINGHFSFTNY
                     QQIFTTKYLKMFAYSILYAALITIITLTISYPAAYYITRSKFQNILLMIMIIPTWINL
                     LLKTYAFIGLLSHDGVINQFFHLFNLPSFNLLFTTGAFLVVASYIYIPFMILPIFNSM
                     KAIPNNLLQASSDLGASPFYTFRKVIMPLTKEGVMTGIQVTFIPSLSLFMITRLIAGN
                     KVINIGTAIEEQFLTIQNYGMGSTIAIFLIVFMAFILIITKSSNGRG"
     gene            15971..16780
                     /locus_tag="POB55_001064"
     CDS             15971..16780
                     /locus_tag="POB55_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6500037.1"
                     /translation="MKWYGKLYIGILLAILYIPIFFLMFYSFNSAGNMIHFEHFTLEH
                     YQSLFQNDHLMSVIFNTIAVALLAASISTVIGTFGAIAIYYLRNKKFKVTLLTLNNVL
                     MVSSDVVIGASFLIMFTTIGHFTGLGLGFWTVLISHIAFCIPIVVIIVLPQLYEMNNN
                     MLNAARDLGATEPQLLSNIIIPNILPSIIGGFFMALTYSLDDFTVSFFVTGNGFSVLS
                     VEVYAMARKGISMEINAISTLLFAVIVLGILGYYLIQYVINKKKLIKRGVK"
     gene            16780..>17842
                     /locus_tag="POB55_001065"
     CDS             16780..>17842
                     /locus_tag="POB55_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HDI6500038.1"
                     /translation="MKRFLQLIIGALVVGMLCLTLSHWFKSKEQVHTNQKIYVYNWGE
                     YIDPELIKKFEKETGIQVVYETFDSNEAMEAKIRNGGTHYDVAFPSEYTVQKLKRDHL
                     LLPIDHNKVPNIKNLDSDYMNMSFDRGNKYSLPYFFGTVGILYNKEKYPNESFDSWKS
                     LYNPKFKNQILLVDGAREIIGMSLNKLGYNLNDRNSHHLKEAERDLTKLAPQVRGVVG
                     DEITMMLQQNEGNIAVVWSGVAAPLVQEGDKYNYVIPKEGSNLWFDNMVIPKTAQNKE
                     GAYKFMNFLLDAKNNKQNTEFVGYATPNKAARQLLPKEIKDDHRFYPTKKEQERLEVY
                     KDLGPEVLSEYNENFLNFKM"
BASE COUNT         6154 a         2598 c         3266 g         5824 t
ORIGIN      
        1 tggatattta atactttgtt gatttcatca agatcaacat ggtacattgg attaccattc
       61 aacaaaataa acggagttga aaaagcatca aaatctatca tttcgtttcg atattgttga
      121 ttgttgatat ttctctcttc aaaatcaatt tgatgctcat taagataatt ttttacaaat
      181 gtacaaggtg ggcaatcatt ctgcgtataa acgattattt ctgacatgta tcatgctcct
      241 tcgtgtttta ttctaatgta atatacacaa ttatacattg aaattcaaat ttgtgaacac
      301 attgtgaact gacataaatt atacacaatt tcatagcgca taatgtttta aaaatacaca
      361 ttatagcaat tataattaag ttcattgtgt tgtcatcaaa aaaagaaaaa ggtgatgttt
      421 ttaaatggat acagttgaaa tcagtcggtt tttgacagct atgactttag cagttcatat
      481 catttttgca acgattggtg ttggtatgcc tttaatgttc gcaattgcag aatttttagg
      541 tattcgcaaa aatgatcttc aatatatagc tatggccaaa agatgggcta aagcttatac
      601 aattactgta gcagtgggag ttgttacagg tacaattata ggacttcaat tatcattgat
      661 ttggcctaca tttatggaaa tgggtggaca cgttattgca cttccattat ttatggaaac
      721 atttgcgttc ttctttgaag ctattttctt aagtatatat ttatatactt gggatcgttt
      781 taaaaataaa tggacacatt tcttaattag tataccagta attattggtg gctctttctc
      841 agcattcttc attacttcag tgaattcatt tatgaatacg cctgcaggtt ttgagttgaa
      901 gaatggaaag atggtcaatg ttcaacctat agaagcgatg tttaacccat cgtttatagt
      961 tcgatcattt cacgtaatta ctacagccgg tatgacgatg gcatttgtta ttgcatcaat
     1021 agcagctttt aaattattgc gtaatcgtca acctaaagat actgtctacc ataagaaagc
     1081 tttgaaaatg tctatgatag ttggattctt ttcaacatta ctttctatgt tggcagggga
     1141 tttatctgca aaatttttgc ataaattcca acctgaaaaa ttagcagctt atgaatggca
     1201 tttcgataca tcttcccatg ctaaattatt attatttggt gtgttagatg aaaagactca
     1261 gcaagttaaa ggtgcgattg aattacctgg actattgagt ttcttagcag ataatagtgt
     1321 caaaactaaa gtgcaagggt taaatgattt tccaaaaagt ttacatccgc ctatgattgt
     1381 ccattatttc tttgatttaa tggtaacgat gggaatttta tgttttgtca tttcaggtgt
     1441 ttatgtttta actttaatgt ttaaaaagct tagaaagttt tctactcata aatggatgct
     1501 ttacggaata ttattaacag gaccagcttc aatgctagct atagaatttg gatggttctt
     1561 aacagagatg ggtagacagc cttggattgt tcgtggttat atgcgcgtgg cagaagcagc
     1621 aacacaagca ggcggaataa ccttcgttac aattttattt ggcatattgt acatcatttt
     1681 aatgtataca tgtgcatacg tattaattcg tatgtttaaa aataaaccgg cgtatgaaga
     1741 tgtaaatcgt ttagccaaga agcaaggagg agaaatagaa aaatgattta tgcatttata
     1801 ggtataacag tgttatggtt gtttttattt tgctatatca ttattgcttc tatagatttt
     1861 ggggcaggtt tcttcgcatt gcattcaaag ttaactggtg atgaaaagaa aattaatcac
     1921 ttaatttcac gttatttaaa cccagtttgg gaagttacga atgtattctt tgttttcttc
     1981 ttcgtaggat tcgtaggttt ctttccagag tcaatcaaat atttaggcac ggtattgtta
     2041 ataccaggtt caatagcact gattatgata tcgttgagaa acagttttta tgcatttgaa
     2101 aattatggtc aagatacaaa attagcatgg atgatcatgt atggggtaag tggattatta
     2161 attccagctt cattatctac tgctttaact attacagaag gtggctatat taatgttcga
     2221 aacaatgtta tcgatctaga ttgggtgcag ttactattaa gcccatttgc ttggtctgta
     2281 gtattcttgg caattatttc agttttatat atttcatcag gatttttgac atattatgct
     2341 aaaaaagcaa atgacgaacc agcatataat ttaacaagac aatggcacat atttttaggg
     2401 ccgccgatga ttatcatttg tttattcgta tttctatcat tacgcattca aaattctgaa
     2461 catttttatt cagctgtttt tgactattgg tggatgtttg ctataagttt cttattcttt
     2521 gcattagctt cattgttaac attctttaag aaaaaacatg gtttggcttt tgtatttgtt
     2581 attttacaaa tgatgttcgc gttctttggc tatggaatta gtaaattgcc atacctttta
     2641 tatccgtttg taaaaattac agatgcatat gttaatccag aaatgggctg gacattagtg
     2701 attgtcttta ttttaggttt acttttatta cttccatcgt taatattatt attaagatta
     2761 tttgttttcg acaaagaata tgttgaagga aagaaatcat aagttgagac gataccccaa
     2821 ctttcattta cttgcattac tgcatttaga taatttagtt ggggtactca tttataataa
     2881 ttttaaattt atattttcaa tgaattgatg aaaatcattt aatggagggc gagtatgggt
     2941 aaagaatatg tagtcatcgg tctaggtcgt tttggaggta gtatcgttcg tgagttgaat
     3001 gcattagaca tggatgtaat ggccatcgac catgatgaaa atagagtgaa tgaatatagt
     3061 gatatcgcta ctcatgcggt tgttgcagac acaacagatg aagcagttat gaaaagttta
     3121 ggtatccgta actttgatca tgtcattgtg gcaattggtg aaaatattca atcaagtacg
     3181 ttgacgactt taattttaaa agagttaggt gtaaaaaaag taactgctaa agcacaaaat
     3241 gattatcatg caaagatttt aaataaaatt ggagcagata cggttgtgca ccctgagcgt
     3301 gatatgggta gacgtattgc gcataatgtt gcgagtgcaa gtgtacttga ttatcttgag
     3361 ttggcagacg agcattctat tgtagaattg aaagcaactg aaaagatggc ggggcagtct
     3421 atcattgatt tagatataag agcacaatat ggaattaaca ttattgcaat taaacgaggc
     3481 aaagagttca tcatttcacc aaatccaaat attaatttag aaataggtga tattttaatc
     3541 atgattggac atgataatga tttaaatcgc tttgaaaaaa atattgcgac gagataatcg
     3601 ggagtgggac agaaatgata ttttctcaaa atttatttcg tcgtcccacc ccaacttaca
     3661 ttgtctgtag aaattgggaa tccaatttct ctttgttggg gcccacaccc caactcgcat
     3721 tgcctgtaga atttcttttc gaaattctct atgttggggc ccctgactag aattgaaaaa
     3781 agcttgttac aagcgcattt tcgttcagtc aactactgcc aatataactt tgtagagcat
     3841 tgaacattga tttatgtctc aagctcatca tatataaaga aaaagctact aacaatgggt
     3901 ggtgagttta attaaaactt cacttcaaag ttagtagctt tttgattatt tattgtttga
     3961 ttctttttgt tcgtttacct tcataatgac tggtaaaatc attggttttc tagctgtttt
     4021 ttcaaataaa taaggttgta atgtttcaat gatagaagat ttaatctgat gccattgaat
     4081 atctttattt tgatttaact tactaataac atcagttttg attttgcgtt gtgcatcata
     4141 aattaattga cctgattccc tcatatatac aaatcctcga gaaataatgt ctggaccaga
     4201 aagtaattta tttgtattaa aatcaatact aacaacaacg ataactaaac cttcttcaga
     4261 taatagctta cggtctctta ttacaacatt accgatatca ccgataccac taccatcaac
     4321 aagtacatta ccagatggaa tgcgaccagc tttacgtgct gaatcgtgtg ttaaagctaa
     4381 gacatctcca atatcaaaga tgaagacatt atcttcttca acgccgcatt caacaccagt
     4441 ctcaccatgt gcttttaaca tacggtattc accatgaata ggtaagaaat atttcggctt
     4501 gattaatcga agcattaatt gttgatcacc ttgagaacca tgccctgaag tatggatgtt
     4561 agaaatcttg ctatggataa catctgcacc agctttatac aaggaattaa tagttctgtt
     4621 aatacttttt gtattacctg ggataggtga tgaactaaat acaacggtat cttcaggtat
     4681 aattttaatt tgcttatgag taccattagc aattctagat aatgctgcca ttggttcacc
     4741 ttgtgaacca gtacatagta tcaataactc atgcttcggt acggtattaa ttttattagg
     4801 ttcaataaat gtttcaggtg gtgctttaat ataaccaagt tccataccga ttttaatatt
     4861 gttttccatc gaacgaccga acgtaacaat tttacggtta tttttgatag cagcttcaac
     4921 tgcttgttga actcggtaaa tattagaagc gaaggtagca aatataatac gacctttaca
     4981 attacggaag atcttatcta cgttttgacc aacttcacgt tcgcttaaag taaaatcagg
     5041 cacaagtgaa tttgttgagt ctgaaagtaa acatagaacg ccttcttcgc ctaattgagc
     5101 cattttagca atgtttgctg gtttgcctac aggtgtaaaa tcaaatttaa agtcaccggt
     5161 atgaactact tttccttcag gtgtatctac gatgacgcca taagtttcag gaatactatg
     5221 cgtagttaag tagaaagaaa tcgtaaagtg cttagattta atcacactgt cctcattgat
     5281 ttcatttagt ttagcagtac gtaataaatg atgttcttca agtttattac ggattaaacc
     5341 taatgctaaa ggaccaccat aaataggtat attaagttgt tttaatagga agggcacacc
     5401 gcctatatgg tcttcgtgac catgtgttat aaataggcca acaattttat cttggttttg
     5461 aactagatat gtgtagtcag gtataacata atcaatccct aataagttat catctgggaa
     5521 tttgatacct gcatcgataa tgacaatttc gtctttatac tcaactgcat aagtattttt
     5581 accgatttca cctagacctc caagtgcata tacacctact tcatttggat gtaattgttt
     5641 cattatttag caatctccac attaaagtgt tctgagtttt ctttttcgta atctaaatgt
     5701 gcgccctcta atttagtgat aaattcgata ttaaaattac gatctttcaa gtaacgacgt
     5761 acttgttctt ctgtttgagc ttcaacataa agtgattgtg tattttcacg cacaattacc
     5821 tcgtctctgt tatgttgata aaaaacttta aatactgcca tgttaaaaat tcctcctaag
     5881 aatgtttgtt taatttattg ttaaccctag taaaatcgta ttggagtata tatcgataaa
     5941 ttcattccaa tcatctctat aatgtaactt aataacgatt tggttaataa ctaggttcat
     6001 catgtcgttc attttaaaaa attagtgaaa taacactaaa atttcagtta aatcttattt
     6061 tacatgatga atgataataa ataaagcagt ttatctcact agaaatcgaa gtatttcgaa
     6121 taaatgtgca gttctattta ttaaaataaa tgtagagatt ataaattaat gttaagaaag
     6181 aattaaagga ttttatatga aacggtaaaa tttaaatgag gggataaaaa gtttggtaga
     6241 aaggtgaata tatttaactg ttgtttattc gaaaaagttg tacaagaaaa agcttaggac
     6301 ataatattta tcctaagctt tgataattag taaaaatgtg ctttaaactt ctacagcatc
     6361 tgtatgtggt tgtaatgggt gatctttgtc aatgtgatca tagaacatta caccatttaa
     6421 atggtcaatt tcatgttgga aaacaattgc tggatatcct tttagacgta attgtatatc
     6481 attaccttcg atgtctttgg ctttaattgt aattctatta tgacggtgaa ctagaccagc
     6541 aacattatca tcgacactaa ggcaaccttc accagttggt aaataagctt cttgaacgct
     6601 atgacttaca atttttgggt tcacaagcat atagtcataa gatttgccac tgccatcatc
     6661 tggtattaaa acagcaatca tacgtttaga aatattaatt tgaggtgcag ccaaaccaac
     6721 gcctgaacgt aaaccatatc gtttcgcgat ttcctcatct tgactattta ctaaaaactc
     6781 tctcatggcg attaatgttt ctttttcttc tttagttaat ggtaattcta actcagctgc
     6841 tttttgacgc aaagttggat gaccatctct aatgatgtct ttcattgtta acatatattg
     6901 caccttcctt attttaattt gttttagttg aatgacagta aaaaggttgt taagatactc
     6961 atacattttt atgtgtaaat atctacaaag ttaaccaact actgccaatg tttattttag
     7021 atagtatatg taaattttca gatatgctaa ttgcttaaaa aatgattaaa gtgttggttt
     7081 caagcaatga tactttagaa atttatttat catcttgact ttaaaaatta tattataaat
     7141 gacgtaactg tcaacagata tacttagtaa tgaagatgtg taatgtaatt gtttaaaatt
     7201 gatttccaag cagattttat ttatcattta atttaaatag caagtggagg tacaagtaat
     7261 gaaatttgga aaaacaatcg cagtagtatt agcatctagt gtcttgcttg caggatgtac
     7321 tacggataaa aaagaaatta aggcatattt aaagcaagtg gataaaatta aagacgatga
     7381 agaaccaatt aaaactgttg gtaagaaaat tgctgaatta gatgagaaaa agaaaaaatt
     7441 aactgaagat gtcaatagta aagatacagc agttcgcggt aaagcagtaa aggatttaat
     7501 taaaaatgcc gatgatcgtc taaaggaatt tgaaaaagaa gaagacgcaa ttaagaagtc
     7561 tgaacaagac tttaagaaag caaaaagtca cgttgataac attgataatg atgttaaacg
     7621 taaagaagta aaacaattag atgatgtatt aaaagaaaaa tataagttac acagtgatta
     7681 cgcgaaagca tataaaaagg ctgtaaactc agagaaaaca ttatttaaat atttaaatca
     7741 aaatgacgcg acacaacaag gtgttaacga aaaatcaaaa gcaatagaac agaactataa
     7801 aaagttaaaa gaagtatcag ataagtatac aaaagtacta aataaggttg gtaaagaaaa
     7861 gcaagacgtt gatcaattta aataattaat ataatacaga tggtaggaaa caactaatac
     7921 agttcctatt atctgtatct ttttttatta aaacagaact ttttcaaatg gtttaacagt
     7981 cccatttatt tgtggtacaa ttattaagga taaaatgaat ttctatacaa ttatgggaaa
     8041 ggtatggtga attgaatggc tcctaagtta caagcccaat tcgatgcagt aaaagtttta
     8101 aatgatactc aatcgaaatt tgaaatggtt caaattttgg atgagaatgg taacgtcgta
     8161 aatgaagact tagtacctga tcttacggat gaacaattag tggaattaat ggaaagaatg
     8221 gtatggactc gtatccttga tcaacgttct atctcattaa acagacaagg acgtttaggt
     8281 ttctatgcac caactgctgg tcaagaagca tcacaattag cgtcacaata cgctttagaa
     8341 aaagaagatt acattttacc gggatacaga gatgttcctc aaattatttg gcatggttta
     8401 ccattaactg aagctttctt attctcaaga ggtcacttca aaggaaatca attccctgaa
     8461 ggcgttaatg cattaagccc acaaattatt atcggtgcac aatacattca agctgctggt
     8521 gttgcatttg cacttaaaaa acgtggtaaa aatgcagttg caatcactta cactggtgac
     8581 ggtggttctt cacaaggtga tttctacgaa ggtattaact ttgcagcagc ttataaagca
     8641 cctgcaattt tcgttattca aaacaataac tatgcaattt caacaccaag aagcaagcaa
     8701 actgctgctg aaacattagc tcaaaaagca attgctgtag gtattcctgg tatccaagtt
     8761 gatggtatgg atgcgttagc tgtatatcaa gcaactaaag aagcacgtga ccgcgcagtt
     8821 gcaggtgaag gtccaacatt aattgaaact atgacatatc gttatggtcc tcatacaatg
     8881 gctggtgacg atccaactcg ttacagaact tcagacgaag atgctgaatg ggagaaaaaa
     8941 gacccattag tacgtttccg taaattcctt gaaaacaaag gtttatggaa tgaagacaaa
     9001 gaaaatgaag ttattgaacg tgcaaaagct gatattaaag cagcaattaa agaggctgat
     9061 aacactgaaa aacaaactgt tacttctcta atggaaatta tgtatgaaga tatgcctcaa
     9121 aacttagcag aacaatatga aatttacaaa gagaaggagt cgaagtaagc catggcacaa
     9181 atgacaatgg ttcaagcgat taatgatgcg cttaaaactg aacttaaaaa tgaccaagat
     9241 gttttaattt ttggtgaaga cgttggtgtt aacggcggtg ttttccgtgt tactgaagga
     9301 ctacaaaaag aatttggtga agatagagta ttcgatacac ctttagctga atcaggtatt
     9361 ggtggtttag cgatgggtct tgcagttgaa ggattccgtc cggttatgga agtacaattc
     9421 ttaggtttcg tattcgaagt atttgatgcg attgctggac aaattgcacg tactcgtttc
     9481 cgttcaggcg gtactaaaac tgcacctgta acaattcgta gcccatttgg tggtggcgta
     9541 cacacaccag aattacacgc agataactta gaaggtattt tagctcaatc tccaggtcta
     9601 aaggttgtta ttccttcagg cccatacgat gcgaaaggtt tattaatttc ttctattaga
     9661 agtaatgacc cagtcgtata cttagagcat atgaaattgt atcgttcatt ccgtgaagaa
     9721 gtacctgaag aagaatatac aattgacatt ggtaaggcta atgtgaaaaa agaaggtaat
     9781 gacatttcaa tcatcacata cggtgcaatg gttcaagaat caatgaaagc tgcagaagaa
     9841 cttgaaaaag atggttattc tgttgaagta attgacttac gtactgttca accaatcgat
     9901 gttgacacaa ttgtagcttc agttgaaaaa actggtcgtg cagttgtagt tcaagaagca
     9961 caacgtcaag ctggtgttgg tgcagcagtt gtagctgaat taagtgaacg tgcaatcctt
    10021 tcattagaag cacctattgg aagagttgca gcagcagata caatttatcc attcactcaa
    10081 gctgaaaatg tttggttacc aaacaaaaat gacatcatcg aaaaagcaaa agaaacttta
    10141 gaattttaat acattttaaa agttaacgaa gttagcgtat tttagtctca ttgattaaaa
    10201 tgaaatgttt aatttacgaa atcttaggag ggcaaaaacg tggcatttga atttagatta
    10261 cccgatatcg gggaaggtat ccacgaaggt gaaattgtaa aatggtttgt taaagctgga
    10321 gatactattg aagaagacga tgttttagct gaggtacaaa acgataaatc agtagtagaa
    10381 atcccatcac cagtatctgg tactgtagaa gaagttatgg tagaagaagg tacagtagct
    10441 gtagttggtg acgttattgt taaaatcgat gcacctgatg cagaagatat gcaatttaaa
    10501 ggtcatgatg atgattcatc atctaaagaa gaacctgcga aagaggaagc gccagcagag
    10561 caagcacctg tagctactca aactgaagaa gtagatgaaa acagaactgt taaagcaatg
    10621 ccttcagtac gtaaatacgc acgtgaaaaa ggtgttaaca ttaaagcagt ttctggatct
    10681 ggtaaaaatg gtcgtattac aaaagaagat gtagatgcat acttaaatgg tggtgcacca
    10741 acagcttcaa atgaatcagc tgcttcagct acaagtgaag aagttgctga aactcctgca
    10801 gcacctgcag cagtaacatt agaaggcgac ttcccagaaa caactgaaaa aatccctgct
    10861 atgcgtagag caattgcgaa agcaatggtt aactctaagc atactgcacc tcatgtaaca
    10921 ttaatggatg aaattgatgt tcaagcatta tgggatcacc gtaagaaatt taaagaaatc
    10981 gcagctgaac aaggtactaa gttaacattc ttaccttatg ttgttaaagc acttgtttct
    11041 gcattgaaaa aatacccagc acttaacact tcattcaatg aagaagctgg tgaaatcgtt
    11101 cataaacatt actggaatat cggtattgca gcagacactg atagaggatt attagtacct
    11161 gttgttaaac atgctgatcg taagtctatt ttccaaattt cagatgaaat taatgaatta
    11221 gctgttaaag cacgtgatgg taaattaaca gccgatgaaa tgaaaggtgc tacatgcaca
    11281 atcagtaata tcggttcagc tggtggacaa tggttcactc cagttatcaa tcacccagaa
    11341 gtagcaatct taggaattgg ccgtattgct caaaaaccta tcgttaaaga tggagaaatt
    11401 gttgcagcac cagtattagc attatcatta agctttgacc acagacaaat tgatggtgca
    11461 actggccaaa atgcaatgaa tcacattaaa cgtttattaa ataatccaga attattatta
    11521 atggaggggt aaaacatggt agttggagat ttcccaattg aaacagatac tatagtaatc
    11581 ggagcaggtc ctggtggata cgttgcagca attcgtgcag ctcaattagg acaaaaagta
    11641 acaatcgttg agaaaggtaa tcttggtggt gtttgcttaa acgtaggatg tattccttca
    11701 aaagcattac tacatgcttc tcaccgtttt gttgaagcac aacattctga aaacttaggt
    11761 gttattgctg aaagtgtttc tttaaacttc caaaaagttc aagaattcaa atcatcagtt
    11821 gttaataaat taactggtgg tgttgaaggc ttacttaaag gtaacaaagt taacatcgtt
    11881 aaaggtgaag catatttcgt agataacaat agcttacgtg ttatggacga aaagagcgca
    11941 caaacataca actttaaaaa tgcaatcatt gcaacaggtt caagaccaat tgaaattcct
    12001 aatttcaaat tcggtaaacg tgttatcgac tcaacaggtg ctttaaactt acaagaagta
    12061 ccaggtaaat tagttgtagt tggtggagga tacattggat cagaattagg tacagcattt
    12121 gctaactttg gttcagaagt aaccatcctt gaaggtgcta aagatatctt aggtggcttc
    12181 gaaaaacaaa tgacacaacc tgttaaaaaa ggtatgaaag aaaaaggtgt tgaaatcgtt
    12241 actgaagcta tggctaaatc agctgaagaa acagataacg gagttaaagt tacttatgaa
    12301 gctaaaggcg aagagaaaac aatcgaagct gattatgtat tagtaactgt aggtcgtcgt
    12361 ccaaacacag acgaattagg cctagaagaa ttaggtgtta aattcgctga ccgtggatta
    12421 ttagaagttg ataaacaaag ccgtacgtct atcagcaata tctatgcaat tggtgatatc
    12481 gttccaggtt taccacttgc tcacaaagct agctatgaag ctaaagttgc tgctgaagca
    12541 attgatggtc aagctgctga agttgattac attggtatgc cagcagtatg ctttactgaa
    12601 ccagaattag ctacagttgg ttattcagaa gcgcaagcta aagaagaagg tttagcaatt
    12661 aaagcttcta aattcccata tgcagcaaat ggtcgtgcat tatcattaga tgatactaac
    12721 ggatttgtta aacttattac acttaaagaa gatgatactt taatcggtgc tcaagtagtt
    12781 ggtactggtg catcagatat tatctctgaa ttaggtttag caattgaagc tggtatgaat
    12841 gctgaagata tcgcattaac aatccatgca catccaacat taggtgagat gactatggaa
    12901 gcagcagaaa aagctatcgg atacccaatc catacaatgt aataactgat tatctataaa
    12961 gattcagtca ttaaaagctg tagcatatgc tacggctttt ttgttttagg taaagtaatg
    13021 taaggaaatt gatttgagat atcgttaaca tgtgacatgc atgttatact agcgatgcta
    13081 acaaaagaat tgaaatggag ggttcaacaa tggaatatga gtatccaatt gatttagact
    13141 ggagtaatga agagatgatt tcagtgataa atttctttaa tcatgtagag aagtattatg
    13201 aatccggcgt gacggcaggc gactttatgg gtgcatataa aagatttaaa gaaattgtgc
    13261 ctgctaaagc agaggaaaaa caaattttta atactttcga aaaaagtagt ggctataata
    13321 gttacaaagc agttcaagat gtaaaaactc actctgaaga acaaagagta acagctaaaa
    13381 aataattcgt tcgaaattaa tacaatttaa taggaatttt tctttaaaac tattgctaat
    13441 aaagctatat tttgatacct ttatcaagtg ttaaacaaaa tgtttgataa aagtaaactt
    13501 aatatagctt ttttaggtgg aaaaataaat gaacataggt aataaaatta aaaatcttag
    13561 aagaattaaa aatttaacgc aagaagaact tgctgaacgt acagacttat cgaaaggcta
    13621 catttcacaa atagaaagtg aacatgcctc accaagtatg gaaactttct taaatattat
    13681 agaggtgtta ggaacgacgc caagtgaatt ttttaaagac agtgaaaatg aaaaagtatt
    13741 atacaagaag gaagaacaag ttatttatga tgagtatgat gaaggttata tattaaattg
    13801 gctagtttca aagtcaaatg aatatgatat ggagccatta atattaactt taaagcctgg
    13861 agcatcatat aaaaatttta atccatcaga gtctgatacg tttatttatt gtatgtcagg
    13921 tcagataaca cttaatttag gcaaagagat atatcaagca caagaagaag acgttttgta
    13981 ttttaaagca cgagataatc atcgtttgtc aaacgaatca aacaatgaaa cacgaatact
    14041 tattgtagcg acagcttcat atttataggg gggatcttat ttggaaccgt tattatcatt
    14101 aaaatcagtt agtaaaagct atgatgatct taatatctta gatgacatag atattgatat
    14161 tgaatcagga tacttttata cattattagg tccttcaggt tgtggtaaaa caacaatttt
    14221 aaaattaatt gcagggtttg aatatcctga cagtggtgaa gtgatttatc aaaacaaacc
    14281 aattggtaat ttaccaccaa ataaacgtaa agtgaataca gtctttcaag attatgcatt
    14341 atttccacac ttaaacgtct atgataatat cgcttttggt ttgaaattaa aaaaattatc
    14401 aaaaaccgaa attgatcaaa aagtaactga ggcattaaaa ttagtaaaac tttcaggtta
    14461 tgaaaaaaga aatattaatg aaatgagtgg cggacaaaag caacgtgttg caattgcacg
    14521 tgctatcgta aatgaaccag aaatattatt gttagatgaa tctttatccg cattagattt
    14581 gaaattgcgt actgaaatgc aatatgaatt acgagaattg caatctagat taggtattac
    14641 atttatattt gtaacacatg atcaagaaga agcgttagca ttaagtgact ttctttttgt
    14701 attaaaagat gggaaaattc aacaatttgg cacaccaaca gatatatatg acgaaccagt
    14761 gaatcgattt gtagctgatt ttattggaga atctaatatt gttgaagggc gcatggttag
    14821 agattatgtc gtgaatattt atgggcaaga tttcgaatgt gtcgatatgg gtattcctga
    14881 aaataaaaaa gtagaagtcg ttattcgacc agaagatata tcattaatca aagctgaaga
    14941 aggattattt aaagcaactg ttgattctat gttatttaga ggggtccact atgaaatatg
    15001 ttgtatagac aataaaggtt atgaatgggt aatacaaacg actaaaaaag ctgaagtagg
    15061 cagtgaagtt ggtctttatt ttgatcctga agccattcat attatggttc ctggagaaac
    15121 agaagaagaa tttgataaac gtattgaaag ctatgaggaa gtagacaatg cgtaatacta
    15181 ataaatttct cttaatcccg tatttactat ggatggttat atttattatt gtaccagttg
    15241 tattactcat ttatttttca tttttagata tcaatggaca ttttagtttc acgaattatc
    15301 aacaaatttt tactacaaaa tatttgaaaa tgtttgcata ttcaatttta tatgccgctt
    15361 taataacaat tattaccttg actatcagtt atccagctgc ctattatatt actcgttcga
    15421 aatttcaaaa tatcttatta atgataatga ttattccaac atggataaat ttattgttaa
    15481 agacatatgc ttttataggt ttattaagtc atgatggcgt gattaatcaa tttttccact
    15541 tatttaattt accatcattc aatttgttat ttacaactgg tgcgttttta gtagtggcaa
    15601 gttacattta tataccattt atgattttac ctatatttaa tagcatgaaa gcaattccta
    15661 ataatttatt gcaggcctca agtgatttag gtgccagtcc tttctatact tttagaaaag
    15721 taatcatgcc gttaacaaaa gaaggtgtta tgactgggat tcaagtaacc tttattccat
    15781 cactttcatt atttatgatt acaagattaa ttgcaggtaa taaagtcata aatataggta
    15841 cggcaataga ggaacaattt ttaacaattc aaaattatgg tatgggatca actatagcta
    15901 tattcttaat tgtatttatg gcattcattt taatcattac aaaatcatct aatgggagag
    15961 ggtgaatcat atgaaatggt atggaaagct gtatatcggg atacttttag cgattttata
    16021 catcccaata ttctttttaa tgttctattc attcaattcg gctggtaata tgattcactt
    16081 tgaacatttt acattagagc attatcaatc attatttcaa aatgatcatt taatgtcggt
    16141 catttttaat acgatagctg tagcactttt agcagcctca atttctacag ttattggtac
    16201 atttggtgcc attgctattt attatttaag aaataaaaag tttaaagtaa ctttactaac
    16261 attgaataat gtcttgatgg tatcatccga cgttgtcata ggtgcatcat tcttaattat
    16321 gtttacaacg attggccatt ttactggtct tggtttagga ttttggacag ttctaatatc
    16381 tcatattgca ttttgcatac ctatagttgt gattatcgtc ttaccacaac tgtatgaaat
    16441 gaataataat atgttaaatg ctgcaagaga tttaggagcg actgaaccac aattattaag
    16501 caacattatt attcctaata ttttaccttc tattatagga ggattcttta tggctttaac
    16561 ttattcacta gacgatttca cagtaagttt cttcgttact ggtaatggct ttagtgtgtt
    16621 atcagttgaa gtttatgcta tggcgagaaa aggaattagt atggaaatta atgcgatttc
    16681 aacattactg tttgctgtta ttgtattagg aatactagga tattatttga ttcaatacgt
    16741 gataaataag aaaaaactaa tcaagcgagg tgtaaaataa tgaaacgttt tttacaactc
    16801 attataggtg cattagttgt gggtatgctt tgtcttactt taagtcattg gtttaaatct
    16861 aaagaacaag tgcatacaaa tcaaaaaatt tacgtataca attggggcga atatattgat
    16921 ccagagttaa ttaagaaatt tgaaaaagaa actggcattc aagtcgttta tgaaactttc
    16981 gattcaaatg aagcgatgga agccaaaatt cgcaatggcg gtacacatta tgatgttgct
    17041 tttcctagtg aatatacagt tcaaaaattg aaaagagatc atttattgtt accaatagat
    17101 cataataagg tacctaatat taaaaattta gattcagatt atatgaatat gtcatttgat
    17161 agaggcaata aatattcttt accttatttc tttggaactg taggtatttt atataataaa
    17221 gaaaagtatc caaatgaatc atttgatagt tggaagtcat tgtataatcc taaatttaaa
    17281 aaccaaattt tactagttga cggtgctaga gagattatag gcatgagttt gaataaactt
    17341 gggtataatc ttaatgaccg taattcgcac catttaaaag aagcagagcg agatttaacc
    17401 aaactagcac cacaagtaag aggtgtcgta ggtgatgaaa ttaccatgat gcttcaacaa
    17461 aatgaaggta acatagcggt tgtttggagt ggtgttgcag cacctctagt gcaagaaggg
    17521 gataaatata attatgttat tcctaaagaa ggatcgaatt tatggttcga caatatggta
    17581 attccaaaaa cggcacaaaa taaagagggt gcatataaat ttatgaattt tttattagat
    17641 gctaaaaata acaagcaaaa tacagaattc gtaggctatg caacgccaaa caaggctgct
    17701 cgacaattgt tgcctaaaga gattaaagac gaccatcgtt tttatccgac taagaaagag
    17761 caagaacgcc ttgaagttta taaagattta ggacctgaag ttttaagtga atacaatgaa
    17821 aactttttga atttcaaaat gt
//
LOCUS       DAMZKC010000042        17187 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.042, whole genome shotgun sequence.
ACCESSION   DAMZKC010000042 DAMZKC010000000
VERSION     DAMZKC010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 17187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17187
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.042"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(129..686)
                     /locus_tag="POB55_001066"
     CDS             complement(129..686)
                     /locus_tag="POB55_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HDI6500039.1"
                     /translation="MINFNKTALVLIDLQEGILKMDYAPYTAENVVQNANKLIDVFRK
                     NNGFIAFVRVNFYDGKDVLQPNAMISLPPKEGDDYSRFHHLLDKRDDDFVIDKRHFSA
                     FVGTDLDLQLRRRGIDTIVLGGVATHIGVDTTARDAYQLNYNQFFVTDMMSAQNETLH
                     QFPIDNVFPLMGQTITTNDFLNILN"
     gene            complement(753..2393)
                     /locus_tag="POB55_001067"
     CDS             complement(753..2393)
                     /locus_tag="POB55_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-keto acid decarboxylase family protein"
                     /protein_id="HDI6500040.1"
                     /translation="MKQRIGAYLIDAIHRAGVDKIFGVPGDFNLAFLDDIISNPNVDW
                     VGNTNELNASYAADGYARLNGLAALVTTFGVGELSAVNGIAGSYAERIPVIAITGAPT
                     RAVEHAGKYVHHSLGEGTFDDYRKMFAHITVAQGYITPENATTEIPRLINTAIAERRP
                     VHLHLPIDVAISEIEIPTPFEVTAAKDTDASTYIELLTSKLHQSKQPIIITGHEINSF
                     HLHQELEDFVNQTQIPVAQLSLGKGAFNEENPYYMGIYDGKIAEDKIRDYVDNSDLIL
                     NIGAKLTDSATAGFSYQFNIDDVVMLNHHNIKIDDVTNDEISLPSLLKQLSNISYTNN
                     ATFPAYHRPTSPDYTVGTEPLTQQTYFKMMQNFLKPNDVIIADQGTSFFGAYDLALYK
                     NNTFIGQPLWGSIGYTLPATLGSQLADKDRRNLLLIGDGSLQLTVQAISTMIRQHIKP
                     VLFVINNDGYTVERLIHGMYEPYNEIHMWDYKALPAVFGGKNVEIHDVESSKDLQDTF
                     NAINGHPDVMHFVEVKMSVEDAPKKLIDIAKAFSQQNK"
     gene            complement(2666..4711)
                     /locus_tag="POB55_001068"
     CDS             complement(2666..4711)
                     /locus_tag="POB55_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIABC"
                     /protein_id="HDI6500041.1"
                     /translation="MRKKLFGQLQRIGKALMLPVAILPAAGLLLAIGTAMQGESLQHY
                     LPFIQNGGVQTVAKLMTGAGGIIFDNLPMIFALGVAIGLAGGDGVAAIAAFVGYIIMN
                     KTMGDFLQVTPKNIGDPASGYASILGIPTLQTGVFGGIIIGALAAWCYNKFYNINLPS
                     YLGFFAGKRFVPIMMATTSFILAFPMALIWPTIQSGLNAFSTGLLDSNTGVAVFLFGF
                     IKRLLIPFGLHHIFHAPFWFEFGSWKNAAGEIIHGDQRIFIEQIREGAHLTAGKFMQG
                     EFPVMMFGLPAAALAIYHTAKPENKKVVAGLMGSAALTSFLTGITEPLEFSFLFVAPL
                     LFFIHAVLDGLSFLTLYLLDLHLGYTFSGGFIDYFLLGILPNKTQWWLVIPVGLVYAV
                     IYYFVFRFLIVKLKYKTPGREDKQSQAATASATELPYAVLEAMGGKANIKHLDACITR
                     LRVEVNDKSKVDVPGLKDLGASGVLEVGNNMQAIFGPKSDQIKHEMQQIMNGQVVENP
                     TTMEDDKDETVVVAEDKSATSELSHIVHAPLTGEVTPLSEVPDQVFSEKMMGDGIAIK
                     PSQGEVRAPFNGKVQMIFPTKHAIGLVSDSGLELLIHIGLDTVKLNGEGFTLHVEEGQ
                     EVKQGDLLINFDLDYIRNHAKSDITPIIVTQGNITNLDFKQGEHGNISFGDQLFEAK"
     gene            5296..6351
                     /locus_tag="POB55_001069"
     CDS             5296..6351
                     /locus_tag="POB55_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDI6500042.1"
                     /translation="MTGFSVYLGQPLDEAYIKRMIKQGYQMIFTSVQIPEEDDETKYH
                     YFTKLLNLLKHEQVTYLIDANPSILTPSFYEHLRQYDAQFMIRIDHSTSIEAIEAIMA
                     QGLKCCLNASIISRELLTSLHQQLNDFTLLSFCHNYYPRPDTGLSVDLVNKKNELIYQ
                     FNPKAQIYGFIVGSDLRGPLHKGLPTIEATRHSHPVVAAKLLQETGVSEVLVGDSLIE
                     MRQAKQLIDFCKHRHFTLCIEEVFDTTVTYLFDMCHKVRPDNPENVIRSETSRQICPH
                     SIQPQFTTQRRIGSVTVDNLNNGRYQGEMQIVRQTLSAHDNVNVVAQIIKEDLPLLSC
                     IEPNDTFDFQKTRECKK"
     gene            6351..7247
                     /gene="murQ"
                     /locus_tag="POB55_001070"
     CDS             6351..7247
                     /gene="murQ"
                     /locus_tag="POB55_001070"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDI6500043.1"
                     /translation="MENSTTEARNEATMHLDEMTVEEALITMNKEDQQVPLAVRKAIP
                     QLTKVIKKTIAQYKKGGRLIYIGAGTSGRLGVLDAAECVPTFNTDPHEIIGIIAGGQH
                     AMTMAVEGAEDHKKLAEEDLKNIDLTSKDVVIGIAASGKTPYVIGGLTFANTIGATTV
                     SISCNEHAVISEIAQYPVEVKVGPEVLTGSTRLKSGTAQKLILNMISTITMVGVGKVY
                     DNLMIDVKATNQKLIDRSVRIIQEICAITYDEAMALYQVSEHDVKVATVMGMCGISKE
                     EATRRLLNNGDIVKRAIRDRQP"
     gene            7259..8713
                     /locus_tag="POB55_001071"
     CDS             7259..8713
                     /locus_tag="POB55_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDI6500044.1"
                     /translation="MTKEQQLAERIIAAVGGMDNIDSVMNCMTRVRIKVLDENKVDDQ
                     ELRHIDGVMGVIHDERIQVVVGPGTVNKVANHMAELSGVKLGDPIPHHHNDSEKMDYK
                     SYAADKAKANKEAHKAKQKNGKLNKVLKSIANIFIPLIPAFIGAGLIGGIAAVLSNLM
                     VAGYISGAWITQLITVFNVIKDGMLAYLAIFTGINAAKEFGATPGLGGVIGGTTLLTG
                     IAGKNILMNVFTGEPLQPGQGGIIGVIFAVWILSIVEKRLHKIVPNAIDIIVTPTIAL
                     LIVGLLTIFIFMPLAGFVSDSLVSVVNGIISIGGVFSGFIIGASFLPLVMLGLHHIFT
                     PIHIEMINQSGATYLLPIAAMAGAGQVGAALALWVRCKRNTTLRNTLKGALPVGFLGI
                     GEPLIYGVTLPLGRPFLTACIGGGIGGAVIGGIGHIGAKAIGPSGVSLLPLISDNMYL
                     GYIAGLLAAYAGGFVCTYLFGTTKAMRQTDLLGD"
     gene            8716..9591
                     /locus_tag="POB55_001072"
     CDS             8716..9591
                     /locus_tag="POB55_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDI6500045.1"
                     /translation="MTNILYRIDKQLSDFTKTEKIIADYILKNPHKIIDMTVNDLADV
                     TNVSTASIVRFSRKMTHQGFQELKIAISRYLPEDIATNPHLELIENESVETLKNKMIA
                     RATNTMRFVATNIMDAQIDAICDVLKNARTIFLFGFGASSLTIGDLFQKLSRIGLNVR
                     LLHETHLLVSTFATHDDRDCMIFVTNQGSHSELQSIAQVATHYSIPIITISSTANNPV
                     AQIADYALIYGRTDENEMRMAATTSLFAQLFTVDILYYRFVALNYHAILDCITQSKMA
                     LDNYRKHLATIDFKH"
     gene            complement(9785..10066)
                     /locus_tag="POB55_001073"
     CDS             complement(9785..10066)
                     /locus_tag="POB55_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500046.1"
                     /translation="MVLITYQIILFFIISLSYYLTLNHYMAVTVGNFTSIFGMFAAIL
                     FMYYYLLYKSPEYNQRKRFKHFIHITNLIIIAFSTFVLVHLALKLFFSI"
     gene            10279..13068
                     /locus_tag="POB55_001074"
     CDS             10279..13068
                     /locus_tag="POB55_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HDI6500047.1"
                     /translation="MAYQSEYALENEMMNQLEQLGYERVTIRDNKQLLDNFRTILNER
                     HADKLEGNPLTDKEFQRLLTMIDGKSIFESARILRDKLPLRRDDESEVYLSFLDTKSW
                     CKNKFQVTNQVSVEDTYKARYDVTILINGLPLVQVELKRRGIDINEAFNQVKRYRKQN
                     YTGLFRYIQMFIISNGVETRYFSNNDSELLKSHMFYWSDKQNNRINTLQSFAESFMRP
                     CQLAKMISRYMIINETDRILMAMRPYQVYAVEALIQQATETGNNGYVWHTTGSGKTLT
                     SFKASQILSQQDDIKKVIFLVDRKDLDSQTEEEFNKFAKGAVDKTFNTSQLVRQLNDK
                     SLPLIVTTIQKMAKAIQGNAHLLEQYKTNKVVFIIDECHRSQFGDMHRLVKQHFKNAQ
                     YFGFTGTPRFPENSSQDGRTTADIFGRCLHTYLIRDAIHDGNVLGFSVDYINTFKNKA
                     LKAEDNSMVEAIDTEEVWLADKRVELVTRHIINNHDKYTRNRQYSSIFTVQSIHALIK
                     YYETFKRLNKKLEQPLTIAGIFTFKPNEDDRDGEVPYHSREKLEIMISDYNKKFETNF
                     STDTTNEYFNHISKNVKKGVKDSKIDILIVVNMFLTGFDSKVLNTLYVDKNLMYHDLI
                     QAYSRTNRVEKESKPFGKIVNYRDLKKETDDALRVFSQTNDTDTILMRSYEEYKKEFM
                     DAYRELKMIVPTPHMVDDIQDEEELKRFVEAYRLLAKIILRLKAFDEFEFTIDEIGMD
                     EQENEDYKSKYLAVYDQVKRATAEKNKVSILNDIDFEIEMMRNDTINVNYIMNILRQI
                     DLEDKAEQRRNQEQIRRILDHADDPTLRLKRDLIREFIDNVVPSLNKDDDIDQEYVNF
                     ESIKKEAEFKGFAGERSIDEQALKTISNDYQYSGVVNPHHLKKMIGDLPLKEKRKARK
                     AIESFVAETTEKYGV"
     gene            complement(13215..13814)
                     /locus_tag="POB55_001075"
     CDS             complement(13215..13814)
                     /locus_tag="POB55_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020808034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500048.1"
                     /translation="MSLSKLPLLNHFANIRIQPKLIIKLFLTLIIIVTYTFVSGLSVD
                     YKKLLSEAGLSETSLAQIKIFSQISIFFSRVFSVLFTFLIILVISRIMRSDANTKSIF
                     SATLSYILFTNAISLIILTIQISAGLSLIDYSITSLNIFAKGNRILGTFDLQFFVQAY
                     ILTVMLIGTSKLSIRASTIWGIIYLILIITFSLISISIQ"
     gene            13940..14695
                     /locus_tag="POB55_001076"
     CDS             13940..14695
                     /locus_tag="POB55_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001253191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RND transporter"
                     /protein_id="HDI6500049.1"
                     /translation="MRRKRKWLIVICVLLVLITISQILKHFNYGTESKEAYNTYTVEK
                     ERQLDLQGKAYPREVKTYYKNNQVGTYLGVQVADGQTVKKGDKVNLSITSTGKTGAGI
                     IKQISELPISYEENLSLHENDALHLPESNNDGELANSKSISASPIFKSNNNSELSKYG
                     VIIDDLSLPIRAGYSLEIKVPLNAIKIPKSVLTKGNNVFIVNKNSIVEKRNIRIKRIN
                     GDIIVEKGLKPGDKLIKQPKSTMNDGDKVEISS"
     gene            14692..15372
                     /locus_tag="POB55_001077"
     CDS             14692..15372
                     /locus_tag="POB55_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6500050.1"
                     /translation="MIELKNVYRTFKNGDKNIRVLKDINIHINAGEFIAIMGPSGSGK
                     STLINILGFIDREYHGEYLFEGSNYQTQSDNQLANIRNKTVGFVFQNFKLIHNNTILE
                     NISMPLVYTNMNTIERKNKVMTVLSAVGLDGKGDFTPSKLSGGQQQRVAIARAIINNP
                     KFIIADEPTGALDSDTSKIIMDIFTSLNRDNHTTIILVTHDRKVAEKADRIIHILGGR
                     IQHEEVIK"
     gene            15369..16547
                     /locus_tag="POB55_001078"
     CDS             15369..16547
                     /locus_tag="POB55_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6500051.1"
                     /translation="MRKVLNIFTIAFKSILKNKGRNIFTMIGIIIGISSVITIMSLGN
                     GFKKTAADQFSDAGAGKQEALISFTFKVDEKIKKYPFNQRDIELVNQVDGVLDAKLKE
                     NKEEGIEATITNVQKKSDIFIIKKQNLHSFKVGRGFDKEDNELRKKIVVINDQVAKTV
                     FNNNAIGKSLYIEGQGFEVIGITDKLYSDSSTVIMPENTFNYYMGHLHQGLPTLQIII
                     EDGYNKKTVVKKVESLLNKKGSGSVLGEYTYTDTEEIIKSIDKIFDSITYFVAAVAGI
                     SLFIAGIGVMNVMYISVAERTEEIAIRRAFGAKSRDIELQFLIESILICVTSGFIGLI
                     LGVVFATIIDVLTPDYIKSVVSLSSVIIAVSVSILIGLLFGWIPARAASKKELIDIIK
                     "
     gene            16754..16975
                     /locus_tag="POB55_001079"
     CDS             16754..16975
                     /locus_tag="POB55_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cyclic bacteriocin"
                     /protein_id="HDI6500052.1"
                     /translation="MLKLHKKIAWTGIKGSAITTFITALYNGSSVWNALAVAGIAFGG
                     GAGTAVAALGRATVMRFIKRWGVRKTAAW"
BASE COUNT         5985 a         2541 c         3284 g         5377 t
ORIGIN      
        1 gggcgtaagt taagaacgat ttaacagata tagaatatgt taaatataga aggttagaca
       61 acccgacgta ttaatacttt gtgaaataaa gatccccctc aacatgatcg aaggggggaa
      121 gtatatgttt agttcaatat atttagaaag tcgtttgtag ttattgtttg tcccatcaat
      181 gggaatacat tatctattgg aaattgatgt agcgtttcgt tttgtgcact catcatatct
      241 gtaacaaaaa actgattgta gtttaattga taggcatctc gcgctgtcgt atctacgcca
      301 atatgcgttg cgacaccacc aagaacaatc gtatcaattc ctcgacgtcg caattgtaag
      361 tccaaatctg ttcctacaaa tgcactaaaa tgtcgtttgt ctatgacaaa atcgtcatct
      421 ctcttgtcta ataaatgatg gaaacgactg tagtcgtcgc cttcttttgg tggtaatgag
      481 atcattgcat ttggttgcaa tacatcttta ccatcataga aattcacgcg aacaaaagcg
      541 ataaagccat tgttttttct aaaaacatct attaatttat tagcgttttg aacgacattt
      601 tcagctgtat atggggcata atccatttta agaatacctt cttgcaggtc gattaacact
      661 aaagcggttt tattaaaatt aatcatctta tatcagagtc ctattcgttt aataaagtat
      721 tcgcttaaac ttataccctg tatacgatga aattatttat tttgttgtga aaaagcttta
      781 gcgatatcga tgagtttctt cggtgcgtct tcgacagaca ttttgacttc gacaaaatgc
      841 atcacatcgg gatgaccatt aattgcatta aacgtgtctt gtaaatcttt tgatgattca
      901 acgtcatgaa tttcaacatt tttaccacca aatacagctg gtaaagcttt ataatcccac
      961 atgtgaattt cattataagg ttcatacatg ccgtgaataa gtcgttctac cgtatagccg
     1021 tcattattaa tcacaaataa taccggttta atatgctgtc taatcatagt tgaaatagct
     1081 tgaacagtta gttgcaatga gccatcacca attaataata agttacgacg atctttgtct
     1141 gctaattgtg aacctaatgt tgcaggtaat gtatagccga tagaacccca taacggttgc
     1201 cctataaaag tattgttttt gtataatgct aaatcataag caccaaagaa tgatgtacct
     1261 tgatcagcaa tgatgacatc atttggtttt aagaaatttt gcatcatttt aaaataagtt
     1321 tgttgtgtta atggttctgt gccaacagta taatcgggtg atgttggacg atgatacgca
     1381 gggaacgttg cgttattcgt atatgaaata ttggataact gttttaacaa tgatggtaga
     1441 gatatttcat catttgtaac atcgtcaatt ttgatattgt gatgatttaa cataacgaca
     1501 tcatcgatat tgaattggta tgaaaaacct gctgttgctg aatctgttaa tttggctcca
     1561 atatttaaaa ttaaatcgct gttgtccaca taatctcgta ttttatcttc ggcaattttc
     1621 ccatcgtaaa tacccatata atatggattt tcctcattaa aagcaccttt tcctaatgaa
     1681 agttgtgcta ctggtatctg tgtttgattt acaaaatctt ctaattcttg atgaaggtga
     1741 aaactgttga tttcatgtcc agtaatgatg ataggctgct tcgattgatg caatttagat
     1801 gttaataact ctatatatgt tgatgcatcc gtatctttgg ctgccgttac ttcaaatggt
     1861 gtcggtatct caatttcaga gattgcgaca tcgattggta aatgtaaatg aactgggcgt
     1921 ctttcggcga ttgctgtatt aattaaacgt ggtatttcgg ttgttgcatt ttcaggtgtg
     1981 atataacctt gtgcaacggt tatatgtgca aacatttttc ggtagtcgtc aaatgtacct
     2041 tcaccaagtg agtgatgtac atatttaccg gcatgttcaa cagcacgtgt cggcgcacct
     2101 gtaatcgcaa tgacaggtat gcgttcagca tatgaacctg cgataccgtt gacggcactt
     2161 aattcgccaa caccaaatgt agtaactaat gcagcgagtc cattaagacg ggcataaccg
     2221 tccgctgcgt aacttgcgtt taattcattt gtatttccta cccaatctac attgggattg
     2281 ctgataatat cgtctagaaa agcgagatta aaatcaccag gaacaccaaa aattttatcg
     2341 acgcctgctc gatgaatagc gtcaattaag taagctccaa tgcgttgttt cataaaaaaa
     2401 ccaccttttc tctatattta cacttaaaat gatagcctcg aatatagtgt aaggtcaagg
     2461 aagttgactt tgttgtgaaa acgctaacaa tagtgaacaa ttaatatttt ttaggaaatg
     2521 agtatgaagg ggggctttaa ttgatgatct taaaattcaa aatttaaggg gagagcgaac
     2581 tatcaatgat gctgcaaaat acaatagcaa agaaaaaaca ggcgcgtcac cttatgaagg
     2641 tataggcacc tgtttatagt aagcattatt tagcttcaaa taattgatcg ccaaatgaaa
     2701 tgttgccatg ttcaccttgt ttaaaatcaa ggtttgtaat gtttccttgt gtcacgataa
     2761 taggcgtaat atcactcttt gcatgattgc ggatgtagtc taaatcaaag ttgattaata
     2821 aatcaccttg tttaacttct tgaccttcct caacatgtaa agtaaagcct tctccgttta
     2881 atttaacagt gtctaaaccg atgtggatta ataattctaa accactatct gatacaagac
     2941 caattgcatg ttttgttggg aaaatcattt gtactttacc gttgaatggt gcacgaactt
     3001 caccttgtga aggtttgata gcgataccgt cacccatcat tttttcgctg aacacttgat
     3061 caggcacttc tgataatggt gttacttcac cagttaatgg tgcatgcacg atatggctca
     3121 attcgcttgt tgcagattta tcttctgcaa caacaacagt ttcgtcttta tcgtcttcca
     3181 tagtagtagg attttctact acttgaccat tcataatctg ttgcatttca tgtttgattt
     3241 ggtcagattt aggaccaaaa attgcttgca tattattgcc gacttctaat acaccagatg
     3301 cgcctaaatc tttcaaacca ggaacatcaa ctttagattt gtcgttaact tcaacacgta
     3361 gacgtgtgat acaagcgtct aaatgtttaa tgtttgcttt gccacccata gcttctaata
     3421 ctgcatatgg taattcagtt gctgaagcag tagccgcttg tgattgttta tcttcacgac
     3481 ctggtgtttt gtattttaat tttacaatta agaatcggaa tacgaagtag taaataactg
     3541 cgtatacaag acctacagga ataactaacc accattgtgt cttattaggt agtataccga
     3601 gtaagaagta gtcgatgaaa ccacctgaga atgtataacc tagatgaaga tctaataagt
     3661 acaatgttaa gaatgataaa ccatcaagta ctgcgtgaat aaagaataat aatggtgcta
     3721 caaataagaa tgagaattct aatggttctg taataccagt taagaatgat gttaaagcag
     3781 cagaacccat taaacctgct actactttct tattttcagg tttagctgtg tgataaattg
     3841 ctaaagctgc tgcaggtaaa ccgaacatca taacagggaa ttcaccttgc atgaatttac
     3901 cagctgtcaa atgtgcgcct tcacgaattt gttcgataaa gatacgttgg tcaccgtgaa
     3961 taatttcacc agctgcattt ttccatgaac caaactcgaa ccagaacggt gcgtggaaaa
     4021 tgtgatgtag accgaatgga attaataaac gcttgatgaa accaaataag aatacggcaa
     4081 caccagtatt tgaatctaat aatcctgtac tgaatgcatt taatcctgat tgaatcgttg
     4141 gccaaattaa tgccattggg aatgctaaaa taaatgatgt tgtagccatc ataataggta
     4201 cgaaacgctt accagcgaag aaacctaaat aagatggtaa gttaatgtta tagaacttgt
     4261 tataacacca agctgccagg gccccgatta taataccgcc gaacacacct gtttgtaatg
     4321 ttgggatacc taaaatgcta gcgtaaccac tcgctggatc accaatattc ttaggtgtaa
     4381 cttgtaaaaa gtcgcccatt gttttgttca tgattatgta accgacgaat gctgcgatag
     4441 ctgctacgcc atcaccgcca gctaatccga ttgcgacacc taatgcgaaa atcataggca
     4501 agttatcaaa aatgatacca ccagcacctg tcattaattt agcgacagtt tgtacgccac
     4561 cattttgtat aaacggcaag tagtgttgta atgattcacc ttgcatagct gtaccgatag
     4621 ctaataacag acccgctgct ggtaaaattg caacaggtaa cattagcgct ttaccaatac
     4681 gttgcaattg accgaaaagt ttcttcctca cttgtccaac ctccaaagtt gtatgtaata
     4741 tttaagattc aataaaaaag acacgagcaa atagactgtg ttggtaacca tcacagctta
     4801 atttaactca tgcctaatct tacttagtaa cacgttggtg tatgtaatta atgtaaaaga
     4861 agcaattgac taagtagtat ataaaactga tacgttagtt tatctagctt accatcacat
     4921 cttattgaat cttatttgtt tgcgactcct attttagcat ggtgaagtat gcgttttcaa
     4981 tacaattatt aaattcaatt tcaaatctct taattattca cagattatta ttaaaacata
     5041 atataagata atcctgaata aaatgtataa aagttattgt aataatccag cagtagcagc
     5101 tattatcagc acttaaaaga aaggataatc tttgatggtg taataattaa cttgtgacag
     5161 aaatatgtaa gattattaca atgaacaggc tttaactgat taatattgta atgtgttttc
     5221 tcttatagta aaatgtaagc gattacacaa ctcaactata ttttcctaat aattatattg
     5281 ttaaggaggg ctactttgac aggcttttca gtgtatttag gacaaccttt agatgaagcg
     5341 tatattaagc gaatgattaa acaaggttac caaatgattt ttacatctgt acaaatacca
     5401 gaagaagatg acgagacaaa atatcattat ttcacaaaac tactcaattt attaaaacat
     5461 gaacaagtga cttacctcat agatgctaat ccatctatat taacaccatc tttttatgag
     5521 catcttcgac aatatgatgc gcaatttatg attcgtatcg atcatagtac atcaattgag
     5581 gcaatcgaag cgataatggc acagggttta aagtgctgtt tgaatgcaag tattatttcc
     5641 cgggaattgt taacaagctt acatcaacaa ttgaatgatt ttacattact ttcattttgt
     5701 cataactatt atccaagacc agatacggga ttatctgttg acttggtcaa taagaaaaat
     5761 gaactcattt atcaatttaa tccaaaggca caaatatatg gttttattgt agggagtgat
     5821 ttgcgaggtc ctttgcataa aggcttgcca acaattgaag caacgagaca tagtcatcct
     5881 gtcgttgcag ctaaattatt acaagaaact ggtgtatctg aagtgttagt tggagactca
     5941 ttgattgaaa tgaggcaggc aaaacaactt atagattttt gcaagcatag gcatttcacg
     6001 ttatgtattg aagaagtgtt tgatacgaca gtgacttacc ttttcgatat gtgtcataaa
     6061 gtacgcccgg ataatccgga aaatgtcatt cgttcggaaa cgtcaagaca aatatgtcca
     6121 cattcgattc aaccacagtt tacgacgcaa cgacgcattg gttcagtaac cgttgataat
     6181 ttgaataacg gacgttatca aggcgaaatg caaattgtga gacaaacgct tagtgcacat
     6241 gacaatgtga atgttgttgc acaaattatt aaagaagact taccactgtt aagttgtatc
     6301 gagccgaatg atacatttga ttttcaaaaa actagggagt gtaagaagtg atggaaaata
     6361 gtacgaccga agcgcgtaat gaagcgacga tgcatcttga tgaaatgact gtggaagagg
     6421 ctttaattac gatgaataaa gaagatcagc aagtcccgtt agcagttcga aaggcaatac
     6481 cacaattgac aaaagtaatt aaaaaaacaa ttgcacagta taaaaagggt ggacgattga
     6541 tttatatcgg tgcaggtaca agtggaaggt tgggtgtctt agatgcagcg gagtgtgtac
     6601 ctacattcaa tactgaccct catgaaatta taggtattat tgctggtgga caacatgcta
     6661 tgacgatggc tgtagaaggt gcggaagatc acaaaaaatt agcggaagaa gatttgaaaa
     6721 atatagattt aacatcaaaa gatgtcgtta taggaattgc cgcgagtggc aaaacgccat
     6781 atgttatagg cggtttaaca tttgctaaca caatcggtgc tacaacagta tctatttcat
     6841 gcaatgaaca tgcagttata agtgaaattg cgcagtatcc agtagaagtt aaagttggtc
     6901 cagaagtatt aactggttca acacgtttaa agtctggtac agcacaaaag ttaattttaa
     6961 atatgatttc aaccatcaca atggttggtg tcggaaaagt ttacgataac ctcatgattg
     7021 atgttaaagc aaccaatcaa aaactgatcg accgttcagt gcgtattatt caagaaatat
     7081 gtgctatcac atatgatgaa gcaatggcgt tatatcaggt atctgagcat gatgtgaaag
     7141 ttgcgacagt tatgggtatg tgtggcattt ctaaggaaga agcaacaaga cggttattaa
     7201 acaatggtga cattgttaaa cgagcaatca gagatagaca accttaggag ggatttaaat
     7261 gaccaaagaa caacaacttg cagaacgaat tattgctgca gtaggtggta tggataatat
     7321 agatagtgtc atgaactgta tgacacgtgt gcgtattaaa gtattagatg agaataaagt
     7381 agatgaccaa gaactaaggc atattgatgg tgtcatgggt gttatacacg atgaacgtat
     7441 tcaagttgtg gttggacctg gtacagtcaa taaagtggct aatcatatgg cggaattaag
     7501 tggtgttaaa ctaggtgacc caataccaca ccatcacaat gatagtgaaa aaatggacta
     7561 taaatcatat gcagctgata aagcaaaggc gaataaggaa gcgcataaag caaaacaaaa
     7621 gaatggtaag ttgaataaag tattgaaatc aattgccaat atctttatac cgttgattcc
     7681 tgcatttatt ggagctggat taattggtgg tattgcagca gtactgagta acttaatggt
     7741 ggcaggctat atttcaggtg cttggattac gcaacttata acagtattta atgtcattaa
     7801 agacggtatg ttagcatact tagctatttt cactggtatt aatgcggcta aagaatttgg
     7861 tgcgacacca ggacttggtg gcgtgattgg tggtacaacg ttattaacgg gtattgctgg
     7921 taaaaatatt ttaatgaatg tcttcactgg agaaccattg caacctggac aaggtgggat
     7981 tattggcgtt atttttgccg tttggatttt aagtattgtc gaaaagagat tacataaaat
     8041 tgtgccaaat gcgattgata ttattgtaac gccgactatt gcattgttga ttgtaggact
     8101 attaactatc tttatcttta tgccattagc aggttttgtt tcagacagtt tagtttcagt
     8161 agttaacgga attattagta ttggtggcgt atttagtgga tttatcattg gtgcaagctt
     8221 cctaccgtta gttatgttag ggcttcatca tatttttacg ccaattcata tagaaatgat
     8281 taaccaatca ggtgctactt acttattgcc aattgcagcg atggctggtg ctggacaagt
     8341 aggtgccgca ttagcacttt gggtaagatg taaacgcaac acaacattac gtaatacttt
     8401 aaaaggtgca ttgccagttg gtttcctagg tatcggagaa ccattaatct atggtgtgac
     8461 tttgccatta ggtcgacctt tcttaactgc ttgtattggt ggtggtattg gtggcgctgt
     8521 aataggtgga attggacata ttggtgccaa agcaataggc ccaagtggtg tgtcactatt
     8581 accattaatc tcagataata tgtatttagg ttatattgca ggattacttg ctgcgtatgc
     8641 tggtggattc gtttgtacat atttatttgg aacgacaaag gcgatgcgac agacagattt
     8701 gttgggtgat taatgatgac aaatatttta tatcgcattg ataagcagtt gagtgatttt
     8761 acgaagacag aaaagataat cgctgattac attttaaaga atccacataa aatcattgat
     8821 atgactgtga atgatttggc agatgttacg aatgttagta cagcatcaat tgttagattt
     8881 agtcggaaaa tgacacatca aggttttcaa gagctaaaga ttgcgatatc tcgatactta
     8941 cccgaagata ttgcaaccaa tccacattta gaattgattg aaaatgaatc tgtagaaact
     9001 ttgaaaaata aaatgattgc tagagcaacg aatacgatgc gatttgtagc tactaatatt
     9061 atggatgcgc aaattgatgc aatttgtgat gtgttgaaaa atgccaggac aatattttta
     9121 tttggatttg gcgcatcgag tttgactatt ggtgatcttt ttcaaaagtt atctcgtatt
     9181 ggcttaaatg tcaggttatt acatgaaacg catttacttg tgtcaacatt tgcgacgcat
     9241 gatgatagag attgcatgat ttttgtgacg aatcaaggta gtcatagtga attgcagtca
     9301 attgcacagg tggccacaca ttacagtatt cccatcataa ctatatctag tacagctaat
     9361 aatccagtgg ctcaaattgc agactatgca ttgatttatg gcagaactga tgaaaatgaa
     9421 atgcgtatgg cggctacaac gtcactattt gcacagttat tcacggtaga tatattgtac
     9481 tatcgatttg tagcattaaa ttatcatgcg attctagatt gtataaccca atcgaaaatg
     9541 gcacttgata attacaggaa gcatcttgcg acgatagatt ttaaacatta gatattgtgt
     9601 gcgttctaac ataaagttag ttgcaacgta aacaattgaa gtttttgatg tgattcagtt
     9661 gcttgatgaa aaattgttgc tacaagtttg taaaactggc atagaggcta ttttgaaaat
     9721 gataaaggcg tgacaaatta aagcaaagtt cagatttgag ctttgctttt ttcatagatg
     9781 gaaattaaat gctgaagaat aattttaatg ctaaatgaac taatacgaag gtgctaaaag
     9841 caattattat caaattagtg atatgaatga aatgtttaaa tcgtttgcgt tgattgtatt
     9901 cgggactttt atagagtagg tagtagtaca taaagagtat ggctgcgaac atgccgaata
     9961 ttgaagtgaa gttacctaca gtgactgcca tgtaatgatt taaagttaaa tagtaactta
    10021 gactaataat aaaaaataaa atgatttgat aggtaattaa caccattcaa gtccctccat
    10081 taatcgtaga caaaaaattt atcaatgatt tgattatttg tattcaaatt ttagtatacg
    10141 acttacctca aagacaaata ttaaaagata agacaataaa attagaataa atttacgttt
    10201 gaagataaaa aacaaacatt tttcattaaa gtttatgata tatttagagc aatagaaagt
    10261 gtatggaagg ggatatgaat ggcataccaa agtgaatacg cattagaaaa tgaaatgatg
    10321 aatcaacttg aacaattggg ttacgaaaga gtaacgatac gtgataataa gcaattgctt
    10381 gataatttta gaacgatttt aaatgagcgt catgcggaca aattagaagg caatccctta
    10441 acagataaag aatttcaacg tctgttaacg atgattgatg gaaaaagtat tttcgagagt
    10501 gcccgtattt tacgtgataa attaccactt agacgtgatg atgagtctga ggtttatttg
    10561 tcgtttttag atacgaaaag ttggtgtaaa aataagtttc aagtgacgaa tcaagtatct
    10621 gtcgaggata catataaagc acgttatgat gtaacgatat taatcaacgg actacccctt
    10681 gtccaagttg aattgaaacg tcgaggtatt gatattaatg aggcgtttaa ccaagtaaaa
    10741 cgttaccgca aacaaaatta cacaggctta ttccgctaca tacaaatgtt tatcattagt
    10801 aatggtgttg aaacgcgata cttttctaat aatgatagcg aactattgaa gagtcacatg
    10861 ttttattgga gtgataaaca gaataaccgt atcaatacat tgcaatcgtt tgctgagtca
    10921 tttatgagac cttgtcaatt agctaagatg atatcgcgct atatgattat taatgaaaca
    10981 gatagaatac tgatggcaat gcgtccgtat caagtgtatg cggtagaagc acttattcaa
    11041 caagcgactg agacagggaa taatggatat gtatggcata caactggaag tggtaagacg
    11101 ttgacttctt ttaaagcgag tcagatttta tcacagcaag atgacattaa gaaagttatc
    11161 tttttggttg accgtaaaga cttggatagt caaacagaag aggaatttaa taaatttgct
    11221 aagggtgctg tagacaaaac ttttaatacc tcgcaactgg tacgccaact aaatgataaa
    11281 agtttgccac ttattgtaac gacgattcaa aaaatggcta aagcgattca agggaatgcc
    11341 catttattag aacagtataa aacgaataaa gttgtattta ttattgatga gtgtcatcgc
    11401 agtcaatttg gtgacatgca tcgtctagtt aaacaacatt tcaaaaatgc ccaatacttt
    11461 ggattcactg gtacgccacg ttttccagaa aatagtagtc aagatggtag aacaactgca
    11521 gatattttcg gtagatgctt acatacgtat ttaattagag atgccattca tgatggtaat
    11581 gtacttggtt tctcagttga ctatattaat acttttaaaa ataaagcttt aaaagcagaa
    11641 gataacagca tggttgaagc aattgatacg gaagaagtat ggttagcgga taaacgtgtg
    11701 gaattagtaa cacgacatat catcaataat catgataaat atacacgtaa tcgtcaatat
    11761 tcaagtatat ttacagtcca aagtattcac gcgcttatta aatattatga gacatttaag
    11821 cgacttaaca aaaagttgga acaaccgtta acgatagctg gtatatttac gtttaaacct
    11881 aatgaagatg atcgtgatgg tgaagtgcca tatcattcac gtgaaaaatt agagataatg
    11941 attagtgatt ataataaaaa gttcgagacg aatttttcaa cagacacaac taatgagtat
    12001 tttaatcata tttcaaaaaa cgttaaaaag ggcgttaaag atagtaaaat tgatatctta
    12061 atcgttgtta atatgttctt aactggtttt gatagtaaag tactgaacac tttatatgtt
    12121 gataagaatt taatgtatca tgatttaatt caagcgtatt cacgtacaaa tagggttgaa
    12181 aaagaatcaa agccatttgg taaaattgta aactatcgtg acttgaaaaa agagacagac
    12241 gatgcactga gagtattctc acaaacaaat gatacggata caattttaat gcgcagttat
    12301 gaagagtata aaaaagaatt tatggacgct tatcgtgagc ttaaaatgat tgtgccgaca
    12361 ccacacatgg ttgatgacat tcaagatgaa gaagagctaa agcgctttgt tgaagcttat
    12421 cgtttattag ctaaaataat attacgttta aaagcatttg acgagtttga gtttacaatt
    12481 gatgaaattg gaatggatga acaagagaat gaagactata aaagtaaata tttagctgtg
    12541 tacgatcaag taaaaagagc gacggctgag aaaaataaag tatccatttt aaatgatatt
    12601 gatttcgaaa tagaaatgat gcgtaatgat acgattaatg tgaattatat tatgaatata
    12661 ttgagacaaa ttgatcttga agacaaagcg gaacaacgtc gtaaccaaga acaaattaga
    12721 cgcattttag atcatgcaga tgatccgaca ttgaggttaa aacgagatct aattagagaa
    12781 ttcatcgaca atgttgtacc ttctttaaat aaggatgatg atatcgatca agaatatgtt
    12841 aatttcgaaa gtattaaaaa agaagcggag ttcaaaggat ttgctggaga gagatctatc
    12901 gatgaacaag ccctaaaaac aatttcaaat gactaccagt atagtggtgt tgtaaaccca
    12961 catcacctta aaaaaatgat tggtgatttg ccattgaaag aaaagcgtaa agcaagaaaa
    13021 gccattgaat ctttcgtggc agaaacaact gaaaaatacg gtgtgtaatg attcagcccc
    13081 ctcgctagat tagtgtaggg ggcattatta tttcattaga aaaggctcat ttgttaaatt
    13141 catcttatca agaatataac gaatgttcct tgttttaaac ttattaatta atagagacat
    13201 tttatctgaa aatatcactg aatgctaata ctaatcaaag aaaaagtgat aattaaaata
    13261 agatatataa taccccaaat agtggatgct cgtatagaaa gtttcgacgt accaattagc
    13321 atgactgtaa gtatgtatgc ttgtacaaaa aattgtaagt cgaaagtacc cagaatgcga
    13381 tttcctttag caaaaatatt taaactagta atagaatagt caattaatga taaaccggca
    13441 cttatttgga tggtaagtat aattaaacta atagcatttg taaacagtat gtagcttaac
    13501 gttgcagaga atatagattt tgtattagca tctgagcgca taattctaga gataactaga
    13561 atgattaaaa atgtaaataa tacgctgaaa actcttgaga aaaaaatact aatttgacta
    13621 aaaattttta tttgtgctaa tgatgtctct gagagtcctg cttcactcaa taacttttta
    13681 tagtccacag atagtccact aacaaatgta tatgtaacaa ttattatcaa agtcagaaac
    13741 agcttaatga ttaactttgg ttgaatgcgg atatttgcaa agtgattaag caaaggtagt
    13801 tttgataatg acatatagac ctcctaatga tataattctc ctatatttta tcataaaaat
    13861 aatataaaaa atatatttta ttaaaaataa gtataaaaaa tggtttgaag agtatttaac
    13921 atttatagga ggacataaat tgaggcgtaa aaggaaatgg ctaattgtta tttgtgtatt
    13981 attagtattg attacgatat cacaaatttt gaaacatttt aactatggaa ctgagagtaa
    14041 agaagcatac aatacatata cggttgaaaa agaaagacaa ttagatttac aaggtaaggc
    14101 atatccacgt gaagttaaaa catattataa aaataatcag gttggaacat atttgggtgt
    14161 gcaagtagcg gacgggcaaa cagtcaaaaa aggagacaaa gttaacttaa gtattacgag
    14221 tacgggtaaa acaggagcgg ggattataaa acaaatctct gagttgccta ttagttatga
    14281 agagaattta agtcttcacg aaaatgatgc cctgcattta ccagaaagta acaacgatgg
    14341 ggaattagca aattcaaaat cgatttcggc atctccaata tttaagagta ataacaactc
    14401 tgaattatca aagtatggag ttataattga tgatcttagt ctaccaatac gtgcaggtta
    14461 ttcgttggaa attaaagtcc cattaaatgc gattaaaata cctaaatcag tattaacaaa
    14521 aggcaataat gtttttattg ttaataaaaa tagtattgtg gaaaaacgga atattcgtat
    14581 aaaaagaatc aatggtgaca ttattgtaga aaaggggtta aaacctggag acaagttaat
    14641 taaacaacct aaaagtacaa tgaatgatgg tgataaggtt gagatttcgt catgatagaa
    14701 ttaaagaatg tttataggac ttttaaaaac ggtgataaaa atattcgagt tttaaaagac
    14761 attaatatac atatcaatgc aggtgaattc atagcgatta tgggaccatc aggctcaggg
    14821 aagagcacac tgattaatat cttaggattt atagatcgag agtatcatgg tgaatatttg
    14881 tttgaaggaa gtaattatca gacacaatct gataatcaat tggcaaacat aagaaataaa
    14941 acagttggat ttgtttttca aaattttaaa ctaattcata ataatactat tttagaaaat
    15001 ataagtatgc cactggtata tacaaatatg aatacgatag aacgaaagaa taaagttatg
    15061 actgtgctca gtgctgtagg cctagatggc aaaggtgatt ttactcctag taaactatca
    15121 ggcggtcaac aacaacgtgt tgcaattgcg cgagccatta taaataatcc aaagtttatt
    15181 attgcagatg agccaacggg tgctttggat tcagatacat ctaaaattat tatggacata
    15241 tttacatcat taaaccgaga taatcatact acaattattt tagtcacaca tgatcgtaaa
    15301 gtagcagaaa aagctgatcg aataattcat attttgggtg gtcgtataca acatgaagag
    15361 gtgattaaat gaggaaagtt ttaaatattt ttacaatagc ttttaaatca atattgaaaa
    15421 ataaaggtag aaatatattt acaatgatag gtataatcat cggtatttct tctgttatta
    15481 cgataatgtc tttgggaaat ggctttaaaa aaactgctgc tgatcaattt tcagatgctg
    15541 gcgccggaaa acaagaagcg ttgattagct ttacttttaa agttgatgag aaaatcaaaa
    15601 agtatccttt taatcaaaga gatatagagt tggtaaatca agttgatggc gtactagatg
    15661 cgaagctaaa ggaaaataaa gaagaaggaa ttgaagccac aataactaat gtccagaaaa
    15721 aaagtgacat cttcattata aaaaaacaaa atttacactc ttttaaagta ggtagagggt
    15781 ttgataaaga ggataatgaa ttacgtaaga aaatagtggt tataaatgat caagtagcaa
    15841 aaactgtatt caataataat gctattggta aatcgctata tattgaggga cagggatttg
    15901 aagtcatagg aattacggat aaactatatt ctgactcatc taccgttata atgcctgaaa
    15961 atacattcaa ctactatatg ggacacttac atcaaggtct gcctacctta cagataatta
    16021 ttgaagatgg ttacaataaa aaaactgtag ttaaaaaagt agaatcacta ttaaataaaa
    16081 aaggatcggg ctctgtttta ggtgagtata catatacaga cactgaagaa attatcaaaa
    16141 gtattgataa aatttttgat agtattactt attttgtagc agctgttgcc ggtatttcac
    16201 tttttattgc tggtattgga gttatgaatg tcatgtatat atctgtagct gaaagaacag
    16261 aagaaattgc gatacgacgt gcatttggtg caaaaagtcg agatattgaa ctacaatttt
    16321 taatagagag cattttaata tgtgtgacaa gtggttttat tggacttatt ttaggtgttg
    16381 tgtttgcaac aataattgat gtattaacac cagattatat taaaagtgta gtaagtttga
    16441 gttctgtcat aattgcggtt agtgtgtcga tattaattgg acttcttttt ggatggatac
    16501 cagctcgcgc agcatctaag aaagagctca tagatattat aaaatagtaa atttaaaatg
    16561 gtaaaagtac tgaagactaa ttatcatatt aaactgacta tcaacaccaa cgatggaaat
    16621 gcgtattaga ataaaagtaa gatatgtatt gcaaaatgta aattatatat ttaatttacg
    16681 caaaaaataa catattactt ttaagatgta aaaaaatatt atataatatt tttgagaagg
    16741 aggtggaagg cctatgctaa aattacataa aaaaatagct tggacaggaa tcaaaggttc
    16801 agctataact acatttatta ctgccttata taatggaagt agtgtttgga acgcgctagc
    16861 tgttgctggt attgcttttg gtggtggcgc tggtactgcg gttgcagctc ttggtcgtgc
    16921 aacagtaatg agatttatca aacgttgggg cgtacgaaaa acagctgctt ggtaaaagat
    16981 aatctaattc tttataagag aggctttctt ttttgggaaa gcctcttttt aaaaaaggag
    17041 attcaatgaa taattcaata ttttatacct atttaaaatt taatcttaga tattttatcc
    17101 gtagaaaatt tgaaattgta aaaaggtcgc gacttaatgt tactacaaaa atactgtttt
    17161 taattaaatt gattttttcg tcactat
//
LOCUS       DAMZKC010000043        17022 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.043, whole genome shotgun sequence.
ACCESSION   DAMZKC010000043 DAMZKC010000000
VERSION     DAMZKC010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 17022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17022
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.043"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            126..2657
                     /gene="secA"
                     /locus_tag="POB55_001080"
     CDS             126..2657
                     /gene="secA"
                     /locus_tag="POB55_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDI6500053.1"
                     /translation="MGFLSKILDGNNKEIKQLGKLADKVIALEEKTAILTDEEIRNKT
                     KQFQTELADIDNVKKQNDYLDKILPEAYALVREGSKRVFNMTPYKVQIMGGIAIHKGD
                     IAEMRTGEGKTLTATMPTYLNALAGRGVHVITVNEYLSSVQSEEMAELYNFLGLTVGL
                     NLNSKTTEEKREAYAQDITYSTNNELGFDYLRDNMVNYSEDRVMRPLHFAIIDEVDSI
                     LIDEARTPLIISGEAEKSTSLYTQANVFAKMLKQDEDYKYDEKTKAVHLTEQGADKAE
                     RMFKVENLYDVQNVDVISHINTALRAHVTLQRDVDYMVVDGEVLIVDQFTGRTMPGRR
                     FSEGLHQAIEAKEGVQIQNESKTMASITFQNYFRMYNKLAGMTGTAKTEEEEFRNIYN
                     MTVTQIPTNKPVQRNDKSDLIYISQKGKFDAVVEDVVEKHKAGQPVLLGTVAVETSEY
                     ISNLLKKRGIRHDVLNAKNHEREAEIVAGAGQKGAVTIATNMAGRGTDIKLGEGVEEL
                     GGLAVIGTERHESRRIDDQLRGRSGRQGDKGDSRFYLSLQDELMIRFGSERLQKMMSR
                     LGLDDSTPIESKMVSRAVESAQKRVEGNNFDARKRILEYDEVLRKQREIIYNERNSII
                     DEEDSSQVVDAMLRSTLQRSINYYINTADDEPEYQPFIDYINDIFLQEGDITEDDIKG
                     KDAEDIFEVVWAKIEAAYQSQKDILEEQMNEFERMILLRSIDSHWTDHIDTMDQLRQG
                     IHLRSYAQQNPLRDYQNEGHELFDIMMQNIEEDTCKFILKSVVQVEDNIEREKTTEFG
                     EAKHVSAEDGKEKVKPKPIVKGDQVGRNDDCPCGSGKKFKNCHGK"
     gene            2699..2803
                     /locus_tag="POB55_001081"
     CDS             2699..2803
                     /locus_tag="POB55_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500054.1"
                     /translation="MLWEESFYFTSVKYFKKCEEVVKRCYVLSFKKSV"
     gene            2972..4082
                     /gene="prfB"
                     /locus_tag="POB55_001082"
     CDS             join(2972..3043,3045..4082)
                     /gene="prfB"
                     /locus_tag="POB55_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014667688.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HDI6500055.1"
                     /translation="MELSEIKRNIDKYNQDLTQIRGSLDLENKETNIQEYEEMMAEPN
                     FWDNQTKAQDIIDKNNALKAIVNGYKTLQAEVDDMDATWDLLQEEFDEEMKEDLEQEV
                     INFKAKVDEYELQLLLDGPHDANNAILELHPGAGGTESQDWANMLFRMYQRYCEKKGF
                     KVETVDYLPGDEAGIKSVTLLIKGHNAYGYLKAEKGVHRLVRISPFDSSGRRHTSFAS
                     CDVIPDFNNDEIEIEINPDDITVDTFRASGAGGQHINKTESAIRITHHPSGIVVNNQN
                     ERSQIKNREAAMKMLKSKLYQLKLEEQAREMAEIRGEQKEIGWGSQIRSYVFHPYSMV
                     KDHRTNEETGKVDAVMDGDIGPFIESYLRQTMSHD"
     gene            4236..4343
                     /locus_tag="POB55_001083"
     CDS             4236..4343
                     /locus_tag="POB55_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500056.1"
                     /translation="MYLLKQHMQCAFSNITYGDFQGIELIVENVLNYMV"
     gene            4491..5330
                     /locus_tag="POB55_001084"
     CDS             4491..5330
                     /locus_tag="POB55_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDI6500057.1"
                     /translation="MKKTLTVTVSSVLAFLALNNAAHAQQHGTQVKTPVQHNYVSNVQ
                     AQTQSPTTYTVVAGDSLYKIALEHHLTLNQLYSYNPGVTPLIFPGDVISLVPQNKVKQ
                     TKAVKTPVRKASQAKKVVKQPVQQASKKVVVKQAPKQAVAKTVNVAYKPAQVQKSVPT
                     VPVAHNYNKSVANRGNLYAYGNCTYYAFDRRAQLGRSIGSLWGNANNWNYAAKVAGFK
                     VDKTPEVGAIFQTAAGPYGHVGVVESVNPNGTITVSEMNYAGFNVKSSRTILNPGKYN
                     YIH"
     gene            5504..6154
                     /locus_tag="POB55_001085"
     CDS             5504..6154
                     /locus_tag="POB55_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDI6500058.1"
                     /translation="MGVHQYFKRLSDMERLIRLPGKFKYFEHNVAAHSFKVTKIAQYL
                     ATVEEYHGRKINWKSLYEKALNHDFAEVFTGDIKTPVKYASSELKKLFSQVEEEMVET
                     FIEEEIPLQYRDVYKQRLQEGKDDSLEGQILSVADKIDLLYETFGEIQKRNPEELFFE
                     IYEMSLETIIQFDHLASVQDFINNIIPEMLTENFIPRTELRETTMNILNKRKEENE"
     gene            6151..6387
                     /locus_tag="POB55_001086"
     CDS             6151..6387
                     /locus_tag="POB55_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbA family protein"
                     /protein_id="HDI6500059.1"
                     /translation="MIWYFSAAFFPCVLVVLFSVITRSKWVGTILTLILIGASIYKEY
                     FHNEWIIFIDVVSLLAGYLIIDQLEFHKHQDEDR"
     gene            6656..8641
                     /gene="uvrB"
                     /locus_tag="POB55_001087"
     CDS             6656..8641
                     /gene="uvrB"
                     /locus_tag="POB55_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002473941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HDI6500060.1"
                     /translation="MVEHYPFKIHSDFEPQGDQPQAIEEIVEGIKAGKRHQTLLGATG
                     TGKTFTMSNVIKEVGKPTLIIAHNKTLAGQLYSEFKEFFPENRVEYFVSYYDYYQPEA
                     YVPSTDTFIEKDASINDEIDQLRHSATSALFERDDVIIIASVSCIYGLGNPEEYKDLV
                     VSVRVGMEMDRSELLRKLVDVQYTRNDIDFQRGTFRVRGDVVEIFPASKEELCIRVEF
                     FGDEIDRIREVNYLTGEVLKEREHFAIFPASHFVTREEKLKVAIERIEKELEERLKEL
                     RDENKLLEAQRLEQRTNYDLEMMREMGFCSGIENYSVHLTLRPLGSTPYTLLDYFGDD
                     WLVMIDESHVTLPQVRGMYNGDRARKQVLVDHGFRLPSALDNRPLKFEEFEEKTKQLV
                     YVSATPGPYEIEHTDKMVEQIIRPTGLLDPKIEVRPTENQIDDLLSEIQTRVERNERV
                     LVTTLTKKMSEDLTTYMKEAGIKVNYLHSEIKTLERIEIIRDLRMGTYDVIVGINLLR
                     EGIDIPEVSLVVILDADKEGFLRSNRSLIQTIGRAARNDKGEVIMYADKMTDSMKYAI
                     DETQRRREIQMKHNEKHGITPKTINKKIHDLISATVENDENNDKAQTVIPKKMTKKER
                     QKTIDNIEKEMKQAAKDLDFEKATELRDMLFELKAEG"
     gene            8649..11495
                     /gene="uvrA"
                     /locus_tag="POB55_001088"
     CDS             8649..11495
                     /gene="uvrA"
                     /locus_tag="POB55_001088"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDI6500061.1"
                     /translation="MKEPSIVVKGARAHNLKDIDIELPKNKLIVMTGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLGQMDKPDVDTIEGLSPAISIDQKTTSKNPRSTVAT
                     VTEIYDYIRLLYARVGKPYCPNHNIEIESQTVQQMVDRIMELEARTKIQLLAPVIAHR
                     KGSHEKLIEDIGKKGYVRLRIDGEIVDVNDVPTLDKNKNHTIEVVVDRLVVKDGIETR
                     LADSIETALELSEGQLTVDVIDGEDLKFSESHACPICGFSIGELEPRMFSFNSPFGAC
                     PTCDGLGQKLTVDVDLVVPDKDKTLNEGAIEPWIPTSSDFYPTLLKRVCEVYKINMDK
                     PFKKLTERQRDILLYGSGDKEIEFTFTQRQGGTRKRTMVFEGVVPNISRRFHESPSEY
                     TREMMSKYMTELPCETCHGKRLSREALSVYVGGLNIGEVVEYSISQALNYYKNIDLSE
                     QDQAIANQILKEIISRLTFLNNVGLEYLTLNRASGTLSGGEAQRIRLATQIGSRLTGV
                     LYVLDEPSIGLHQRDNDRLINTLKEMRDLGNTLIVVEHDDDTMRAADYLVDIGPGAGE
                     HGGQIVSSGTPQKVMKDKKSLTGQYLSGKKRIEVPEYRRPASDRKISIRGARSNNLKG
                     VDVDIPLSIMTVVTGVSGSGKSSLVNEVLYKSLAQKINKSKVKPGLYDKIEGIDQLDK
                     IIDIDQSPIGRTPRSNPATYTGVFDDIRDVFAQTNEAKIRGYQKGRFSFNVKGGRCEA
                     CKGDGIIKIEMHFLPDVYVPCEVCDGKRYNRETLEVTYKGKNIADILEMTVEEATQFF
                     ENIPKIKRKLQTLVDVGLGYVTLGQQATTLSGGEAQRVKLASELHKRSTGKSIYILDE
                     PTTGLHVDDISRLLKVLNRLVENGDTVVIIEHNLDVIKTADYIIDLGPEGGSGGGTIV
                     ATGTPEDIAQTKSSYTGKYLKEVLERDKQNTEDK"
     gene            12102..13034
                     /locus_tag="POB55_001089"
     CDS             12102..13034
                     /locus_tag="POB55_001089"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HDI6500062.1"
                     /translation="MLTTEKLVETLKLDLIAGEEGLSKPIKNADISRPGLEMAGYFSH
                     YASDRIQLLGTTELSFYNLLPDKDRAGRMRKLCRPETPAIIVTRGLQPPEELVEAAKE
                     LNTPLIVAKDATTSLMSRLTTFLEHALAKTTSLHGVLVDVYGVGVLITGDSGIGKSET
                     ALELVKRGHRLVADDNVEIRQINKDELIGKPPKLIEHLLEIRGLGIINVMTLFGAGSI
                     LTEKRIRLNINLENWNKQKLYDRVGLNEETLSILDTEITKKTIPVRPGRNVAVIIEVA
                     AMNYRLNIMGINTAEEFSERLNEEIIKNSHKSEE"
     gene            13040..13879
                     /locus_tag="POB55_001090"
     CDS             13040..13879
                     /locus_tag="POB55_001090"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDI6500063.1"
                     /translation="MGIVFNYIDPVAFNLGPLSVRWYGIIIAVGILLGYFVAQRALVK
                     AGLHKDTLVDIIFYSALFGFIAARIYFVIFQWPYYVENPSEIIKIWHGGIAIHGGLIG
                     GFIAGVIVCKVKNLNPFQIGDIVAPSIILAQGIGRWGNFMNHEAHGGPVSRAFLEKLH
                     LPNFIIENMYINGQYYHPTFLYESIWDVAGFIILVNIRKHLKLGETFFLYLTWYSIGR
                     FFIEGLRTDSLMLTSNIRVAQLVSILLILISISLIVYRRIKYNPPLYSKVGALPWPTK
                     KVK"
     gene            13887..14372
                     /locus_tag="POB55_001091"
     CDS             13887..14372
                     /locus_tag="POB55_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDI6500064.1"
                     /translation="MRKFLSKTHHHTNPLWRVYRLVKFSKVFKNVIIIEFSKFIPSMV
                     LKRHIYKQLLNINIGNQSSIAYKVMLDIFYPELITIGSNSVIGYNVTILTHEALVDEF
                     RYGPVTIGSNTLIGANATILPGITIGDNVKVAAGTVVSKDIPDNGFAYGNPMYIKMIR
                     R"
     gene            14380..15819
                     /locus_tag="POB55_001092"
     CDS             14380..15819
                     /locus_tag="POB55_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDI6500065.1"
                     /translation="MAQKNNNVIPMTFDDAFYRKMAKQKFKQREYKRAAEYFEKVLEL
                     SPDDLEIQIDYAQCLVQLGIAKKAEHLFYDNIIYNRHLEDSFYELSQLNIEVNEPNKA
                     FLFGINYVIVSDDQDYRDELDQMFDVKYQSEEQIELEAQLFVVQILFQYLFSQGRLKD
                     AKNYVLHQPQEVQDHRVVRNLLAMCYLYLGEYDTAKALYEALLQEDSTDIYALCHYTL
                     LLYNTKENEQYQKYLKILNKVVPMNDDESFKLGIVLSYLKQYRASQQLLYPLYKKGKF
                     LSIQMYNALAYNYYYLGEEDESHYYWDKLKQISKVEIGHAPWVIENSKEVFDQHILPL
                     LQSDDSHYRLYGIFLLDQLNGKEIVMTESIWQVLENLNNYEKLYLTYLVQGLTLNKLD
                     FIHRGLLTLYQNELFVSENDLMVAWINQGELIIAEKVDLTDVEPYIGAFIYLYFKNQP
                     RNVTKKQITTWLGITQYKLNKMIEFLLSI"
     gene            15886..16821
                     /gene="trxB"
                     /locus_tag="POB55_001093"
     CDS             15886..16821
                     /gene="trxB"
                     /locus_tag="POB55_001093"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDI6500066.1"
                     /translation="MTEIDFDIAIIGAGPAGMTAAVYASRANLKTVMIERGIPGGQMA
                     NTEEVENFPGFEMITGPDLSTKMFEHAKKFGAVYQYGDIKSVEDKGEYKVINFGNKEL
                     TAKAVIIATGAEYKKIGVPGEQELGGRGVSYCAVCDGAFFKNKRLFVIGGGDSAVEEG
                     TFLTKFADKVTIVHRRDELRAQRILQDRAFKNDKIDFIWSHTLKSINEKDGKVGSVTL
                     TSTKDGSEETHEADGVFIYIGMKPLTAPFKDLGITNDVGYIVTKDDMTTSVPGIFAAG
                     DVRDKGLRQIVTATGDGSIAAQSAAEYIEHLNDQA"
BASE COUNT         6200 a         2323 c         3344 g         5155 t
ORIGIN      
        1 gaagtatgtc ataaatatat ttatattgat tctacaaatg agtaaataag tataattttc
       61 taactataaa tgataagata tattgttgta ggccaaacag ttttttagct aaaggagcga
      121 acgaaatggg atttttatca aaaattcttg atggcaataa taaagaaatt aaacagttag
      181 gtaaacttgc tgataaagta atcgctttag aagaaaaaac ggcaatttta actgatgaag
      241 aaattcgtaa taaaacgaaa caattccaaa cagaattagc tgacattgat aatgtcaaaa
      301 agcaaaatga ttatttagat aaaattttac cagaagcata tgcacttgtt agagaaggct
      361 ctaaacgtgt attcaatatg acaccatata aagttcaaat tatgggtggt attgcaattc
      421 ataaaggtga tatcgctgag atgagaacag gtgaaggtaa aacattaaca gcgacaatgc
      481 caacatactt aaatgcatta gctggtagag gtgttcacgt tattacagtc aatgaatact
      541 tatcaagtgt tcaaagtgaa gaaatggctg agttatataa cttcttaggt ttgactgtcg
      601 gattaaactt aaacagtaag acgacagaag aaaaacgtga agcatacgca caagacatta
      661 cttacagtac taataatgag ctaggttttg attacttacg agataacatg gtgaattatt
      721 ctgaagatag agtaatgcgt ccattacatt ttgcaatcat tgatgaggtt gactcaattt
      781 taatcgacga ggcacgtacg ccattaatta tttctggtga agctgaaaag tcaacgtcac
      841 tttatacaca agcaaatgtt tttgcgaaaa tgttaaaaca ggacgaagat tataaatacg
      901 atgaaaaaac gaaagctgta catttaacag aacaaggtgc ggataaagct gaacgtatgt
      961 tcaaagttga aaacttatat gatgtacaaa atgttgatgt tattagtcat atcaacacag
     1021 ctttacgtgc gcacgttaca ttacaacgtg acgtagacta tatggttgtt gatggcgaag
     1081 tattaattgt cgatcaattt acaggacgta caatgccagg ccgtcgtttc tcggaaggtt
     1141 tacaccaagc tattgaagcg aaggaaggcg ttcaaattca aaatgaatct aaaactatgg
     1201 cgtctattac attccaaaac tatttcagaa tgtacaataa acttgcaggt atgacaggta
     1261 cagctaaaac tgaagaagaa gaatttagaa atatttataa catgacagta actcaaattc
     1321 cgacaaataa acctgtgcaa cgtaacgata agtctgattt aatttacatt agccaaaaag
     1381 gtaaatttga tgcagtagta gaagatgttg ttgaaaaaca caaggcaggg caaccagtgc
     1441 tattaggtac tgttgcagtt gagacttctg aatatatttc aaatttactt aaaaaacgtg
     1501 gtatccgtca tgatgtgtta aatgcgaaaa atcatgaacg tgaagctgaa attgttgcag
     1561 gcgctggaca aaaaggtgcc gttactattg ccactaacat ggctggtcgt ggtacagata
     1621 tcaaattagg tgaaggcgta gaggaattag ggggtttagc agtaataggt acagaacgac
     1681 atgaatctcg tcgtattgat gaccagttac gtggtcgttc tggacgtcaa ggtgataaag
     1741 gggatagtcg cttctattta tcattacaag atgaattaat gattcgtttt ggttctgaac
     1801 gtttacagaa aatgatgagc cgactaggtt tagatgactc tacaccaatt gaatcaaaaa
     1861 tggtatcaag agctgtagaa tcagcacaaa aacgtgtaga aggtaataac ttcgacgcgc
     1921 gtaaacgtat cttagaatac gatgaagtat tacgtaaaca acgtgaaatt atctataacg
     1981 aaagaaatag tattattgat gaagaagaca gctctcaagt tgtagatgca atgctacgtt
     2041 caacgttaca acgtagtatc aattactata ttaatacagc agatgacgag cctgaatatc
     2101 aaccattcat cgactacatt aatgacatct tcttacaaga aggtgacatt acagaggatg
     2161 atatcaaagg taaagatgct gaagatattt tcgaagtcgt ttgggctaag attgaagcag
     2221 catatcaaag tcaaaaagat atcttagaag aacaaatgaa tgagtttgag cgtatgattt
     2281 tacttcgttc tattgatagc cattggactg atcatatcga cacaatggat caattacgtc
     2341 aaggtattca cttacgttct tatgcacagc aaaatccatt acgtgactat caaaatgaag
     2401 gtcatgaatt atttgatatc atgatgcaaa atattgaaga agatacttgt aaattcattt
     2461 taaaatctgt agtacaagtt gaagataata ttgaacgtga aaaaacaaca gagtttggtg
     2521 aagcgaagca cgtttcagct gaagatggta aagaaaaagt gaaaccgaaa ccaatcgtta
     2581 aaggcgatca agttggtcgt aacgatgatt gtccatgtgg tagtggtaaa aaattcaaaa
     2641 attgccatgg aaaataaatg atataaaata actccttcca attaaacacc tatagtttgt
     2701 gttatgggag gagtcttttt attttacaag cgttaaatac tttaaaaaat gtgaagaagt
     2761 tgttaaacgt tgttatgtac ttagttttaa aaaatcggtt taggcatatg tcgatgataa
     2821 atgtactgat ttttaacaat aaatgcataa actaattgtc agtgtgctta tatttcttaa
     2881 cattgttatt taacaaaatt atgttaaaat ttagcattat aaaagataca aatcaatgac
     2941 ttgaattgaa atataaatag gagcgaatgc tatggaatta tcagaaatca aacgaaatat
     3001 agataagtat aatcaagatt taacacaaat tagggggtct ctttgactta gagaacaaag
     3061 aaactaatat tcaagaatat gaagaaatga tggcagaacc taatttttgg gataaccaaa
     3121 cgaaagcgca agatattata gataaaaata atgcgttaaa agcaatagtt aatggttata
     3181 aaacactaca agcagaagta gatgacatgg atgctacttg ggatttatta caagaagaat
     3241 ttgatgaaga aatgaaagaa gacttagagc aagaggtcat taattttaag gctaaagtgg
     3301 atgaatacga attgcaatta ttattagatg ggcctcacga tgccaataac gcaattctag
     3361 agttacatcc tggtgcaggt ggcacggagt ctcaagattg ggctaatatg ctatttagaa
     3421 tgtatcaacg ttattgtgag aagaaaggct ttaaagttga aactgttgat tatctacctg
     3481 gggatgaagc agggattaaa agtgtaacat tgctcatcaa agggcataat gcttatggtt
     3541 atttaaaagc tgaaaaaggt gtacaccgac tagtacgaat ttctccattt gattcatcag
     3601 gacgtcgtca tacatcattt gcatcatgcg acgttattcc agattttaat aatgatgaaa
     3661 tagagattga aatcaatccg gatgatatta cagttgatac attcagagct tctggtgcag
     3721 gtggtcagca tattaacaaa actgaatcgg caatacgaat tacccaccac ccctcaggta
     3781 tagttgttaa taaccaaaat gaacgttctc aaattaaaaa ccgtgaagca gctatgaaaa
     3841 tgttaaagtc taaattatat caattaaaat tggaagagca ggcacgtgaa atggctgaaa
     3901 ttcgtggcga acaaaaagaa atcggctggg gaagccaaat tagatcatat gttttccatc
     3961 catactcaat ggtgaaagat catcgtacga acgaagaaac aggtaaggtt gatgcagtga
     4021 tggatggaga cattggacca tttatcgaat catatttaag acagacaatg tcgcacgatt
     4081 aatatatatt ttaaaaccga ggctctaaaa gggcgtcggt ttttggtttt tttaaaggta
     4141 gctaaataaa ttgtaaatta gattttggaa tatgatttgt ttatgaatat ttaagtacaa
     4201 ttcggtagat agagttagaa tatatttttt gaaagttgta tttgttaaaa caacatatgc
     4261 agtgtgcatt tagtaatatt acctatggcg attttcaagg tattgaatta atcgttgaaa
     4321 acgttctcaa ttacatggta tgaatacatt ttacactatg ataaaaggtt gtattctttt
     4381 tatattgtta accatttgat tacatcgtta taacaatagc ttttgacaaa atgtattgtg
     4441 ctatagtatt tgcatactta aaatactaac agcaaaggaa tgacagcaag atgaaaaaaa
     4501 ctcttacagt gacggtttcg tcagtgttag cttttttagc tttaaataat gcagcacatg
     4561 cacaacaaca tggcacacaa gtaaaaacac ctgttcaaca taattatgta tcaaatgttc
     4621 aagcacaaac gcaatcaccg acaacttata cagtagttgc tggcgattca ttatataaga
     4681 ttgcattaga gcatcactta acgttgaatc aattatattc atacaatcct ggtgtaacac
     4741 ctttaatttt tcctggtgac gtgatttcac ttgtgcctca aaataaagtg aaacaaacta
     4801 aagcggttaa aacaccagta agaaaagcaa gccaagctaa aaaggtagta aaacaacctg
     4861 tacaacaagc gtctaaaaaa gtagtagtta agcaagcacc taagcaagca gtagctaaga
     4921 cagttaatgt agcatacaaa cctgctcaag tacaaaaatc agtaccaact gtacctgttg
     4981 cacataacta caataaatca gttgctaaca gagggaactt atatgcttat ggaaactgca
     5041 catattatgc tttcgatcgt cgtgcacaat taggtagaag tataggaagt ttatggggca
     5101 atgcaaataa ctggaattac gcagcaaaag ttgcaggatt taaagtagat aaaacaccag
     5161 aagttggcgc tattttccaa acagctgctg gcccatatgg acatgttggt gttgttgaat
     5221 ctgtaaaccc taatggaaca attactgttt ctgaaatgaa ctatgctgga tttaatgtta
     5281 aatcttcaag aacaatttta aatccaggaa aatataatta catccactaa gtaatatatc
     5341 aagacaagac tatcctctta gcctgtttaa gtaacaggtt gagaggattt tttggtatca
     5401 tttaatcaga gttatataaa gaagatattt aaatgatatt taattgattg atatgtaaaa
     5461 agaaagtata atttatgatt aattaatgga ggaggtaatt gaaatgggtg tacatcaata
     5521 ttttaaaaga ttatcagata tggaaagact tataagatta cctggaaaat ttaaatattt
     5581 tgaacacaat gttgcagcac actcctttaa agtaactaaa attgctcaat atctagcaac
     5641 agttgaagaa tatcatggac gaaagattaa ttggaaaagc ttatatgaaa aagcattaaa
     5701 tcatgatttc gccgaagtgt ttactggtga tataaaaaca cctgttaaat atgcgagtag
     5761 tgaattaaaa aaattatttt cgcaagttga agaagaaatg gtagagacct ttattgaaga
     5821 agaaattcca ttacaatata gagatgttta taagcaacga ctgcaagaag gtaaagatga
     5881 ttcattagaa ggccaaatac tttcagttgc tgataaaatt gatttgcttt atgaaacatt
     5941 tggagaaata caaaaacgta atcccgaaga attatttttc gaaatttatg aaatgagtct
     6001 agaaacaatt attcaatttg accatttagc atctgtacaa gattttatta ataatatcat
     6061 tccagaaatg ttgactgaaa actttatacc tagaacagaa ttaagagaaa caaccatgaa
     6121 cattttaaat aaaagaaaag aggaaaatga atgatatggt attttagcgc agcattcttt
     6181 ccatgtgtct tggtagtatt atttagtgta ataacaagaa gtaagtgggt cggtactatt
     6241 ctgacattaa ttttaattgg tgcctcaatc tataaagagt atttccataa cgagtggatt
     6301 atttttattg atgtagtgtc attattagct ggttatttaa ttatagatca actcgaattt
     6361 cataaacatc aagatgaaga tcgctaagat taactttaaa ataatgtttc aaacaaattt
     6421 gttgaaacaa aatgatgatt aatataatgt gtatttacat actaaaaata acaagataaa
     6481 cgatttgatt taaggcaagc atagttagca caactatgtt tgtttttctt tgttcgacat
     6541 ttttacgaac aaacgtttgc tttttgtgtg actactttgc taaaatatgt aatgagaaag
     6601 caaagagttg agcggaaata taaataatac gttgaaagag gaggcatatg tgacaatggt
     6661 tgaacattat ccttttaaaa tacattctga ttttgagcct caaggtgacc aaccgcaagc
     6721 aattgaagaa atcgtggaag gtattaaagc ggggaaaaga catcaaactt tattaggtgc
     6781 tactggcaca gggaaaacat ttacgatgag taatgttatt aaagaagttg ggaaaccaac
     6841 gttaattatc gcgcataaca aaacattagc aggacaatta tatagtgagt ttaaagaatt
     6901 ttttcctgaa aacagggtgg aatactttgt aagttactat gattattatc aaccagaggc
     6961 atacgtaccg tctactgaca cttttattga aaaagatgcc tcaatcaatg atgaaattga
     7021 tcaactacga cattctgcta caagtgcatt atttgaacgc gatgatgtaa ttattattgc
     7081 tagtgtaagt tgtatatatg gtttaggtaa tcctgaagaa tataaagatt tagtagtaag
     7141 tgttcgagtt ggtatggaaa tggatagaag tgaattactt agaaaacttg tagatgtgca
     7201 atatacacga aatgacatcg atttccaacg aggaacgttt cgagtgcgtg gtgatgtagt
     7261 tgaaatattc ccagcctcta aagaagaact ttgtataagg gttgagtttt tcggcgatga
     7321 gattgaccgt atccgagaag ttaactacct aacaggtgaa gtgttgaaag aaagagaaca
     7381 ttttgcgata ttcccagctt ctcacttcgt aacacgtgaa gaaaagttga aagttgcgat
     7441 tgaacgtatt gaaaaagaat tggaagaacg attgaaagaa ttacgagatg agaataaatt
     7501 actagaagcg caaaggttag aacagcgtac caactatgat ttagaaatga tgcgagagat
     7561 gggattctgt tcaggaattg aaaactattc cgtacattta actttgcggc cactgggttc
     7621 gacaccatat actttattgg attactttgg cgatgattgg ttagtaatga ttgatgaatc
     7681 acatgtgaca ttaccgcaag ttcgaggcat gtataacgga gacagagcgc gtaaacaagt
     7741 tttggtggat catggattta gattaccgag tgcattagat aaccgtccac ttaaatttga
     7801 agaatttgaa gaaaagacaa aacaacttgt gtatgtatct gcaacgcctg gaccatacga
     7861 aattgaacat acggataaga tggttgaaca aattattcgt cctactggtt tactggatcc
     7921 taagattgag gttagaccta ctgaaaatca aattgacgat ttattaagtg aaattcaaac
     7981 aagagttgag cgtaatgaac gcgtacttgt tacaacgctc actaaaaaga tgagtgaaga
     8041 tttaaccaca tacatgaaag aagcgggtat taaagttaat tatctgcatt cagaaatcaa
     8101 gacattagaa cgaattgaaa taattagaga cttacgaatg ggtacatatg atgttatcgt
     8161 aggtattaat ttattaagag agggtattga tataccagaa gtttctctag ttgtcatatt
     8221 agatgcagat aaagaagggt ttttacgttc taaccgctca ttaattcaaa caataggtag
     8281 agctgcgcgt aacgataaag gtgaagtcat tatgtatgcc gataaaatga ctgattcgat
     8341 gaagtatgca attgatgaga cacaacgtcg tcgagaaata cagatgaaac ataatgaaaa
     8401 acatggtatt acacctaaaa caattaataa aaaaatacat gatttaatta gtgctactgt
     8461 tgaaaatgac gaaaataatg acaaagcaca aactgtgata cctaagaaga tgacgaaaaa
     8521 agaacgtcaa aagacaatcg acaatataga aaaagaaatg aaacaagcag cgaaagattt
     8581 agatttcgag aaagctacag aattaagaga tatgttattt gaattaaaag cagaagggtg
     8641 acaagtaaat gaaagaacca tccatagtag taaaaggtgc tcgtgcgcat aacttgaaag
     8701 atattgatat cgaactacct aaaaataaat taattgttat gacaggttta tctgggtcag
     8761 gtaaatcgtc attagcattc gatactatat atgctgaagg acaacgacgt tatgttgaat
     8821 cattaagtgc ctatgcgcgt caatttttag gccaaatgga caaaccagat gttgatacaa
     8881 ttgaaggatt atcgccagca atttcaatag atcaaaaaac aacaagtaaa aatccaagat
     8941 caactgtagc aacagtaaca gaaatatatg attatatacg tttgttatat gcacgtgttg
     9001 gtaaacctta ctgtccaaat cacaatatag aaattgaatc gcaaacagta caacaaatgg
     9061 ttgaccgcat tatggaatta gaggcacgta caaagattca attattagca cctgtcatcg
     9121 ctcatcgtaa aggtagtcat gaaaagctaa tcgaagatat tggtaaaaaa ggttatgtac
     9181 gtttaagaat cgatggcgaa attgttgatg taaatgatgt acctacttta gataagaaca
     9241 agaatcatac aatagaagtt gttgtagacc gattagttgt taaagatgga attgaaacac
     9301 gactagctga ctctatagaa actgccttag agctttcaga aggacaatta acagtcgatg
     9361 tcattgacgg ggaagacctt aagttttcag aaagccatgc ttgtcctata tgtggatttt
     9421 caatcggaga gttagaacca agaatgttta gctttaacag tccttttggt gcttgtccga
     9481 catgtgatgg cttaggccaa aagttaacag tcgatgtaga cttggttgtt cccgacaaag
     9541 ataagacgct aaacgaaggt gcaatagaac cttggatacc gacgagttct gatttttatc
     9601 caacattgtt aaaacgtgtt tgtgaagttt ataaaatcaa tatggataaa ccttttaaaa
     9661 agttaacaga acgtcaacgt gatattttat tgtatggttc tggtgacaaa gaaattgaat
     9721 ttacatttac acaacgtcaa ggtggtacta gaaaacgaac aatggttttc gagggtgtag
     9781 ttcctaatat aagtagacga ttccatgaat ctccttcaga atatacacgt gaaatgatga
     9841 gtaaatatat gactgaacta ccttgcgaaa cttgtcatgg aaagcgattg agtcgtgaag
     9901 cgttatctgt ttatgtaggt ggtttaaata ttggtgaagt agtcgaatat tcaatcagtc
     9961 aagcgctgaa ctattataaa aacattgatt tgtcagaaca agatcaagcg attgcaaatc
    10021 aaatattgaa agaaattatt tcccgactca cttttttaaa taatgtggga cttgaatatt
    10081 taacgttaaa cagagcttca ggtacacttt caggtggtga agcacaacgt attcgattag
    10141 caacgcaaat tgggtcgcgt ttgactggtg tcttatatgt attagatgag ccatcaattg
    10201 gactgcatca aagagataat gatcgattaa ttaatacact taaagaaatg agagatttag
    10261 gaaatacttt aattgtagtt gaacacgatg atgatacaat gcgtgcggct gattacttag
    10321 tggatatagg tcctggtgct ggtgaacatg gagggcagat tgtgtctagt ggtactcctc
    10381 aaaaggtaat gaaagataaa aaatcattaa caggacaata cttgagtggt aagaaacgta
    10441 ttgaagtacc tgaatatcgc agaccggctt cagatcgtaa aatttctata cgtggagcta
    10501 gaagcaacaa tcttaaaggg gttgatgtgg acataccact atcaatcatg acggttgtta
    10561 caggtgtatc aggttctggt aaaagctcat tagtaaatga agtattatac aaatcattag
    10621 ctcaaaaaat taataaatct aaagtaaagc caggattgta cgataagatt gaaggtattg
    10681 atcaacttga taaaattatt gatattgatc aatcaccaat aggtagaacg ccacgctcta
    10741 atccagcaac atatactggt gtgtttgatg atatacgtga tgtgtttgcg caaacaaatg
    10801 aagctaaaat tcgaggatat caaaaagggc gttttagttt taatgtaaaa ggtggacgct
    10861 gtgaagcttg taaaggtgac ggtattatta aaattgaaat gcatttttta cctgatgttt
    10921 atgttccttg tgaagtgtgt gatggtaaac gatataatcg tgagacacta gaggttactt
    10981 acaaaggtaa aaatattgct gacattttag aaatgactgt tgaagaagca acacaatttt
    11041 ttgaaaatat tcctaagatt aagcgcaagt tacaaacact agttgatgtt ggtcttggat
    11101 acgtcacatt aggtcaacaa gctacaacgt tatcaggtgg tgaggctcaa cgtgtgaaac
    11161 ttgcatctga acttcataaa cgttcaactg gtaaatctat ttatatccta gatgaaccga
    11221 caacagggtt acatgttgac gatattagta gattattaaa agtattaaac cgattagttg
    11281 aaaatggtga tactgttgta attattgaac ataacctaga tgttatcaaa acagcagact
    11341 atattataga cttaggtcct gaaggtggta gtggcggtgg tactattgtt gcgactggca
    11401 cacccgaaga tattgctcag acaaagtcat catatacagg aaagtattta aaagaagtac
    11461 ttgaacgaga taaacaaaat actgaagata aataagatta aaagaagtga aggatgttat
    11521 aaatttatcc ttcgcttctt tttattaatt tagtaatgaa tagtagaaag aaaagatgcg
    11581 taaaaagaat tatgttaaga taggttcaat ctagagtagt taaacataaa tcgaactggg
    11641 agtgggacag aaatgataaa gaatcactaa tgatttatta tgtagtggtt ctttgtcatt
    11701 agccacagct attgtgtact taaaaatagg aatgcatgag tgcaactcat gcataagaaa
    11761 tactaatttc taaagaaaaa gtatttcttt atgttggggc cccgccaact tgcattgttt
    11821 gtagaatttc ttttcgaaat tctttatgtt ggggccccgc caactaattc caatatatca
    11881 ttgtagagct taggtcattg atttttggct cggactttta tggcgatatg aaccatgtaa
    11941 attaagcaag caataaatta atgattgata ttgacttgta aaataataac aataatgaac
    12001 aattaatatt tattttagct tttcaatgta gattggtgtt atatttttga tatgataaga
    12061 agagatgtaa gagtagggat aaatacaatt gaggtgaacc catgttaacg acagaaaaac
    12121 tagttgaaac attaaagtta gatttaatcg ctggtgaaga aggactatcg aagccaatta
    12181 aaaatgctga tatatcaaga ccgggcttag agatggcagg ttatttttca cattatgcgt
    12241 cagatagaat acaactatta ggaacaacgg aactatcgtt ttacaattta ttaccagata
    12301 aggatcgcgc aggtcgtatg cgtaaactat gcagaccaga aacgcctgca attattgtga
    12361 cgcgtggatt gcagccacca gaagaattag ttgaagctgc aaaagaatta aataccccac
    12421 ttatagttgc taaagatgcg actacaagtt taatgagtcg cttaacaacg tttttagagc
    12481 atgcacttgc aaagacgaca tctttacatg gtgttttagt agatgtttac ggtgttggtg
    12541 tactaattac cggtgattca ggaataggta aaagtgagac tgcgttggaa ttagttaaac
    12601 gtgggcatag attagtagca gatgataatg tagaaatacg tcaaattaat aaagatgaac
    12661 taatagggaa accaccaaag ttaatagaac atctattaga aatacgtgga ctaggtatta
    12721 tcaatgttat gactttattt ggcgcgggtt caatattaac tgaaaaacga attagattaa
    12781 atattaattt ggaaaactgg aacaagcaaa agttatatga ccgcgtaggt cttaatgaag
    12841 agacgctaag tattttagat actgaaatca ctaaaaaaac aatacctgta agacctggta
    12901 gaaatgttgc ggtaattatt gaggtcgctg caatgaacta tcgattaaat atcatgggca
    12961 ttaacactgc cgaagaattt agtgaaagat taaatgaaga aattatcaag aacagtcata
    13021 agagtgagga gtaggttgaa tgggtattgt atttaactat atagatcctg tggcatttaa
    13081 cttaggacca ctgagtgtac gatggtatgg aattatcatt gctgtcggaa tattacttgg
    13141 ttactttgtt gcacaacgtg cactagttaa agcaggatta cataaagata ctttagtaga
    13201 tattattttt tatagtgcac tatttggatt tatcgcggca cgaatctatt ttgtgatttt
    13261 ccaatggcca tattacgtgg aaaatccaag tgaaattatt aaaatatggc atggtggaat
    13321 agcaatacat ggtggtttaa taggtggctt tattgctggt gttattgtat gtaaagtgaa
    13381 aaatttaaac ccatttcaaa ttggtgatat cgttgcgcca agtataattt tagcgcaagg
    13441 aattggacgc tggggtaact ttatgaatca cgaggcacat ggtggacctg tgtcacgcgc
    13501 ttttttagaa aaattacatt tgccaaattt tataatagaa aatatgtata ttaacggcca
    13561 atattatcat ccaacattct tatatgaatc catttgggat gtcgctggat ttattatctt
    13621 agttaatatt cgtaaacatt taaaattagg agaaacattc tttttatatt taacttggta
    13681 ttcaattggt cgattcttta tagaaggatt acgtacagat agcttaatgc tcacaagtaa
    13741 tattagagtt gcacaattag tatcaattct tttaatttta ataagtataa gtttaattgt
    13801 atatagaagg attaagtata atccaccgtt gtatagcaaa gttggggcgc ttccatggcc
    13861 aacaaaaaaa gtgaagtagt gatattttga gaaaattttt atcaaaaaca catcatcata
    13921 caaacccttt atggcgtgta taccgtcttg ttaaattttc gaaagttttt aagaatgtaa
    13981 ttatcattga attttcgaaa tttattccaa gtatggtact gaaaagacat atatataaac
    14041 aacttttaaa tattaatatc ggtaatcaat cgtcgatagc ttataaagta atgttagata
    14101 ttttttaccc agaactgatt acgattggta gtaacagtgt tattggttac aatgtaacaa
    14161 ttttgacgca tgaagcatta gttgatgaat ttcgttatgg accagtgacg ataggatcta
    14221 acactttgat tggtgcaaat gctaccattt tacccggtat aacgattggt gacaatgtaa
    14281 aagttgcagc tggtacggtt gtttcaaaag atataccgga taatggattt gcatatggca
    14341 accctatgta tataaaaatg attaggaggt gacaatttta tggcgcaaaa gaataataat
    14401 gtaattccaa tgacttttga tgatgcattt tatcgtaaaa tggctaaaca gaagtttaaa
    14461 caaagagaat ataaacgagc tgctgaatac tttgaaaaag tgttagaatt gtcacctgat
    14521 gatctggaaa ttcaaattga ttatgcacaa tgtctagtgc aacttggtat tgctaaaaaa
    14581 gcagaacatt tattttatga caatattatt tataataggc atctagaaga tagcttttat
    14641 gaattgagtc agctcaacat tgaagttaac gaaccaaaca aggcattctt gtttggtatt
    14701 aattatgtta ttgttagcga cgaccaagat tatagagatg aattagatca aatgtttgat
    14761 gtgaaatatc aaagcgaaga acaaattgaa cttgaagctc aattgtttgt agttcaaata
    14821 ctattccaat atcttttttc tcaaggtcga ttaaaagatg caaagaatta tgtcttacat
    14881 caaccacaag aagttcaaga tcatcgtgtc gtacgtaatt tattggcaat gtgttattta
    14941 tatctcggtg aatatgatac ggctaaagca ttgtacgaag cactattaca agaggatagt
    15001 acagatatat atgcattatg ccattatact ttgctacttt ataacactaa ggaaaatgaa
    15061 caatatcaaa aatatttaaa aatattaaac aaagttgtac ctatgaatga cgatgaaagt
    15121 tttaaattag gtattgtatt aagttattta aagcagtatc gtgcatcaca acaattgttg
    15181 taccctttat ataaaaaagg gaaattttta tcaattcaaa tgtacaatgc tttagcatat
    15241 aattattatt atttaggtga agaagacgaa agtcattact actgggataa attgaagcaa
    15301 atttctaaag tggaaattgg acatgcgcct tgggtaattg aaaatagcaa agaagttttt
    15361 gaccaacata ttttgccatt acttcaaagt gatgacagtc attatcgttt atatggtatt
    15421 tttttattgg atcaattaaa tggtaaagaa attgtgatga cggaaagtat ttggcaggtt
    15481 ctggaaaatc taaataatta tgagaaattg tatttaacgt atttagttca aggtttaacg
    15541 ctcaataaat tagacttcat tcatcgcggc ttgttaacgc tttaccaaaa tgaattattt
    15601 gtaagtgaaa atgatttaat ggttgcatgg attaatcaag gtgaactcat aattgctgaa
    15661 aaagtagatt taactgatgt tgagccatat atcggtgcgt ttatttattt gtattttaaa
    15721 aatcaacctc gaaacgttac aaagaagcaa attacaacat ggttaggcat aacacaatat
    15781 aaactgaaca aaatgattga atttctcttg agcatataga tttatgaaaa gttagattta
    15841 ttatataatg cgcataatga ttaataatga ggaggcgtta ataaaatgac tgaaatagat
    15901 tttgatatag caattatcgg tgcaggtcca gctggtatga ctgctgcagt atacgcatca
    15961 cgtgctaatt taaaaacagt tatgattgaa agaggtattc caggcggtca aatggctaat
    16021 acagaagaag tagagaactt ccctggtttc gaaatgatta caggtccaga tttatctaca
    16081 aaaatgtttg aacacgctaa aaagtttggt gcagtttatc aatatggaga tattaaatct
    16141 gtagaagata aaggcgaata taaagtgatt aactttggta ataaagaatt aacagcgaaa
    16201 gcggtcatta ttgctacagg tgcagaatac aagaaaattg gtgttccggg tgaacaagaa
    16261 cttggtggac gcggtgtaag ttattgtgca gtatgtgatg gtgcattctt taaaaataaa
    16321 cgcctattcg ttatcggtgg tggtgactca gcagtagaag agggaacatt cttaactaaa
    16381 tttgctgaca aagtaacaat cgttcaccgt cgtgatgagt tacgtgcaca acgtatttta
    16441 caagatagag cattcaaaaa tgataaaatc gactttattt ggagtcatac tttgaaatca
    16501 attaatgaaa aagacggcaa agtgggttct gtgacattaa cgtctacaaa agatggttca
    16561 gaagaaacac acgaggctga tggtgtattc atctatattg gtatgaaacc attaacagcg
    16621 ccatttaaag acttaggtat tacaaatgat gttggttata ttgtgacaaa agatgatatg
    16681 acaacatcag taccaggtat ttttgcagca ggagatgttc gtgacaaagg tttacgccaa
    16741 attgtcactg ctactggcga tggtagtatt gcagcgcaaa gtgcagcgga atatattgaa
    16801 catttaaacg atcaagctta attcgaggtc gaattaagat gttgagttgt aagttatttg
    16861 gatatttatt ttaatagtgt catcacattg ttaaaataat gtcttacttt taaattaaaa
    16921 caaattatat agaaaactag aacttagtac gtatcatttg tgcgtttcaa tgagttctag
    16981 tttttttata tgttatatta aacttataac tttatgggag tg
//
LOCUS       DAMZKC010000044        17005 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.044, whole genome shotgun sequence.
ACCESSION   DAMZKC010000044 DAMZKC010000000
VERSION     DAMZKC010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 17005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17005
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.044"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(592..1452)
                     /gene="folD"
                     /locus_tag="POB55_001094"
     CDS             complement(592..1452)
                     /gene="folD"
                     /locus_tag="POB55_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HDI6500067.1"
                     /translation="MVAKILDGKQIAKDYRQGLQDQVEALKEKGFTPKLSVILVGNDG
                     ASQSYVRSKKKAAEKIGMISEIVHLEETATEEEVLNELNRLNNDDSVSGILVQVPLPK
                     QVSEQKILEAINPEKDVDGFHPINIGKLYIDEQTFVPCTPLGIMEILKHADIDLEGKN
                     AVVIGRSHIVGQPVSKLLLQKNASVTILHSRSKDMASYLKDADVIVSAVGKPGLVTKD
                     VVKEGAVIIDVGNTPDENGKLKGDVDYDAVKEIAGAITPVPGGVGPLTITMVLNNTLL
                     AEKMRRGIDS"
     gene            1653..2135
                     /gene="purE"
                     /locus_tag="POB55_001095"
     CDS             1653..2135
                     /gene="purE"
                     /locus_tag="POB55_001095"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HDI6500068.1"
                     /translation="MKVAVIMGSSSDWKIMQESCNMLDYFEIPYEKQVVSAHRTPKMM
                     VQFASEARERGINIIIAGAGGAAHLPGMVASLTTLPVIGVPIETKSLKGIDSLLSIVQ
                     MPGGIPVATTAIGAAGAKNAGILAARMLSIQNPSLVEKLNQYESSLIQKVEDMQNELQ
                     "
     gene            2122..3246
                     /gene="purK"
                     /locus_tag="POB55_001096"
     CDS             2122..3246
                     /gene="purK"
                     /locus_tag="POB55_001096"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HDI6500069.1"
                     /translation="MSFNKLKFGATIGIIGGGQLGKMMAQSAQKMGYKVVVLDPSEDC
                     PCRYVAHEFIQAKYDDEKALNQLGQKCDVITYEFENISAQQLKLLCEKYNIPQGYQAI
                     QLLQDRLTEKETLKSAGTKVVPFISVKESTDIDKAIETLGYPFIVKTRFGGYDGKGQV
                     LINNEKDLQEGFKLIETSECVAEKYLNIKKEVSLTVTRGNNNQITFFPLQENEHRNQI
                     LFKTIVPARIDKTAEAKEQVNKIIQSIHFIGTFTVEFFIDSNNQLYVNEIAPRPHNSG
                     HYSIEACDYSQFDTHILAVTGQSLPNSIELLKPAVMMNLLGKDLDLLENEFNEHPEWH
                     LHIYGKSERKDSRKMGHMTVLTNDVNQTEQDMYAKFEGSN"
     gene            3250..3954
                     /locus_tag="POB55_001097"
     CDS             3250..3954
                     /locus_tag="POB55_001097"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDI6500070.1"
                     /translation="MTLLYEGKAKRIFSTNQENELRVEYKDEVTAGNGAKKDTMAGKG
                     RLNNQITSIIFKYLQENGIESHFIKQLSETEQLVKPVKIIPLEVVVRNIASGSITKRL
                     GFENGEVFREPLVEFFYKNDALNDPLITDDHVKLLNIASDEDIEILKSKALKINNVLK
                     QLMDAMNLKLVDFKIEFGKTETGQILLADEISPDTCRIWDKATNANFDKDVYRNNTGS
                     LIETYQIFLNKLEDLK"
     gene            3954..4217
                     /gene="purS"
                     /locus_tag="POB55_001098"
     CDS             3954..4217
                     /gene="purS"
                     /locus_tag="POB55_001098"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HDI6500071.1"
                     /translation="MKTIELHITLQPQVLDTQGQTLTRAVHDLGYAQVNDIRVGKVLY
                     MTVDEVSDEKVHNIITTLSEKLFANTVIEEYSYKVLDDEKENA"
     gene            4219..4890
                     /gene="purQ"
                     /locus_tag="POB55_001099"
     CDS             4219..4890
                     /gene="purQ"
                     /locus_tag="POB55_001099"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HDI6500072.1"
                     /translation="MKFAVLVFPGSNCDRDMFNAAIKSGVEAEYVDYRETSLSGFDGV
                     LIPGGFSFGDYLRSGAMASVAPIISEVKRLAAEGKPVLGVCNGFQILTEIGLLPGALL
                     HNDSHLFISRNEELEIVNNQTAFTNLYEQGEKVIYPVAHGEGHYYCTDEIYQQLKANN
                     QIILKYVNNPNGSYDDIAGIVNKKGNVCGMMPHPERALETLLGTDSGVKLFEAMVKSW
                     REQHV"
     gene            4883..7072
                     /gene="purL"
                     /locus_tag="POB55_001100"
     CDS             4883..7072
                     /gene="purL"
                     /locus_tag="POB55_001100"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HDI6500073.1"
                     /translation="MSKFIEPSVEEIKLEKVYQDMGLSDQEYEKVCDILGRQPNFTET
                     GIFSVMWSEHCSYKHSKPFLKQFPTSGDHVLMGPGEGAGVVDIGDNQAVVFKVESHNH
                     PSAIEPYQGAATGVGGIIRDIVSIGARPINLLNSLRFGELDNKQNQRLLKGVVKGIGG
                     YGNCIGIPTTAGEIEFDERYDGNPLVNAMCVGVINHDMIQKGTAKGVGNSVIYVGLKT
                     GRDGIHGATFASEELTEESESKRPSVQIGDPFVGKKLMEATLEAITFDELVGIQDMGA
                     AGLTSSSSEMAAKGGSGLHLRLEQVPTREPGISPYEMMLSETQERMLLVVEKGTEQKF
                     LDLFDKHELDSAVIGEVTDTNRFVLTYDDEVYADIPVEPLADEAPVYILEGEEKDYNT
                     SKNDYTHIDVKDTFFKLLKHPTIASKHYLYDQYDQQVGANTIIKPGLQASVVRVEGTN
                     KAIASTIDGEARYVYNNPYEGGKMVVAEAYRNLIAVGATPLAMTDCLNYGSPEKKEIY
                     QQLIDSTKGMAEACDILKTPVVSGNVSLYNETKGTSIFPTPVVGMVGLIENVNYLNDF
                     EPQVGDKLYLIGDTKDDFGGSQLEKLIYGKVNHEFESLDLSSEVEKGESIKTAIREGL
                     LSHVQTVGKGGLLITLAKLSAHYGLGLKSSIDITNAQLFSETQGRYVVSVKSGKTLNI
                     DNAIEIGLLTDSDNFKVTTPYTEISENVSDIKQIWEGAIAQCLTTQD"
     gene            7051..8535
                     /locus_tag="POB55_001101"
     CDS             7051..8535
                     /locus_tag="POB55_001101"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDI6500074.1"
                     /translation="MFNYSGLNEECGVFGIWNHPEAAQLTYMGLHSLQHRGQEGAGIV
                     VSDQNELKGERGLGLLTEAIKDDQMERLKGYQHAIGHVRYATSGNKGIENIQPFLYHF
                     YDMSVGICHNGNLINAKSLRQNLEKQGAIFHSSSDTEVIMHLIRRSKAPTFEEALKES
                     LRKVKGGFTFAILTKDALYGAVDPNAIRPLVVGKMKDGTYILASETCAIDVLGAEFVQ
                     DIHAGEYVVINDKGITVKSYTHHTTTAISAMEYIYFARPDSTIAGKNVHAVRKASGKK
                     LAQESPVNADMVIGVPNSSLSAASGYAEEIGLPYEMGLVKNQYVARTFIQPTQELREQ
                     GVRVKLSAVKDIVDGKNIILVDDSIVRGTTIRRIVKMLKDSGANKVHVRIASPEFMFP
                     SFYGIDVSTTAELISASKSPEEIKDYIGADSLAYLSVDGLIESIGLDYDAPYSGLCVE
                     SFTGDYPAGLYDYEANYKAHLSHRQKQYISKNKHFFDSEGNLNV"
     gene            8528..9556
                     /locus_tag="POB55_001102"
     CDS             8528..9556
                     /locus_tag="POB55_001102"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HDI6500075.1"
                     /translation="MSKAYEQSGVNIHAGYEAVERMSSHVKRTMRKEVIGGLGGFGAT
                     FDLSQLNMTAPVLVSGTDGVGTKLKLAIDYGKHDSIGIDAVAMCVNDILTTGAEPLYF
                     LDYIATNKVVPEVIEQIVKGISDACVETNTALIGGETAEMGEMYHEGEYDVAGFAVGA
                     VEKDDYVDSSEVKEGQVVIGLASSGIHSNGYSLVRKLINESGIDLASNFDNRPFIDVF
                     LEPTKLYVKPVLALKKEVSIKAMNHITGGGFYENIPRALPAGYAARIDTTSFPTPKIF
                     DWLQQQGNIDTNEMYNIFNMGIGYTVIVDEKDASRALKILAEQNVEAYQIGHIMKNES
                     TAIELLGV"
     gene            9559..10125
                     /gene="purN"
                     /locus_tag="POB55_001103"
     CDS             9559..10125
                     /gene="purN"
                     /locus_tag="POB55_001103"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDI6500076.1"
                     /translation="MVKIAIFASGSGSNFENIVEHVESGKLENIEVTALYTDHQNAFC
                     IDRAKKHDIPVYINEPKQFDSKAAYEQHLVSLLNEDKVEWIILAGYMRLIGPDLLASF
                     EGKILNIHPSLLPKYKGIDAIGQAYHSGDTITGSTVHYVDCGMDTGEIIEQRQCDIRP
                     DDSKEQLEEKVKKLEYELYPSVIAKIVK"
     gene            10140..11618
                     /gene="purH"
                     /locus_tag="POB55_001104"
     CDS             10140..11618
                     /gene="purH"
                     /locus_tag="POB55_001104"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HDI6500077.1"
                     /translation="MKKAILSVSNKTGIVEFAKALTQLNYELYSTGGTKRILDEANVP
                     VRSVSDLTHFPEIMDGRVKTLHPAVHGGILADRNKPQHLNELSEQHIDLIDMVVVNLY
                     PFQQTVANPDVTMDEAIENIDIGGPTMLRAAAKNYKHVTTIVHPADYQEVLTRLRNDS
                     LDESYRQSLMIKVFEHTAEYDEAIVRFFKGDKETLRYGENPQQSAYFVRTSNAKHTIA
                     GAKQLHGKQLSYNNIKDADATLALVKKFDTPATVAVKHMNPCGVGIGDTIEQAFQHAY
                     EADSQSIFGGIVALNRAVTPELAEQLHSIFLEVIIAPKFTDEALDILKQKKNVRLLEI
                     DMTIDSNEEEFVSVSGGYLVQDKDNYVVPKEEMKVVTEVAPTDEQWEAMLLGWKVVPS
                     VKSNAIILSNNKQTVGIGAGQMNRVGAAKIALERAIEINDHVALVSDGFFPMGDTVEL
                     AAQHGIKAIIQPGGSIKDQDSIDMANKHGIAMVVTGTRHFKH"
     gene            11640..12887
                     /locus_tag="POB55_001105"
     CDS             11640..12887
                     /locus_tag="POB55_001105"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HDI6500078.1"
                     /translation="MNVLVIGAGGREHALAYKLNQSNLVKQVFAIPGNEAMTPIAEVH
                     TEISESDHQAILDFAKRQNVDWVVIGPEQPLIDGLADILRANGFKVFGPNKQAAQIEG
                     SKLFAKKIMEKYNIPTADYKEVERKKDALTYIENCELPVVVKKDGLAAGKGVIIADTI
                     EAARSAIEIMYGDEEEGTVVFETFLEGEEFSLMTFVNGDLAVPFDCIAQDHKRAFDHD
                     EGPNTGGMGAYCPVPHISDDVLKLTNETIAQPIAKAMLNEGYQFFGVLYIGAILTKNG
                     PKVIEFNARFGDPEAQVLLSRMESDLMQHIIDLDEGKRTEFKWKNESIVGVMLASKGY
                     PDAYEKGHKVSGFDLNENYFVSGLKKQGDTFVTSGGRVILAIGKGDNVQDAQRDAYEK
                     VSQIQSDHLFYRHDIANKALQLK"
     gene            complement(13152..13958)
                     /locus_tag="POB55_001106"
     CDS             complement(13152..13958)
                     /locus_tag="POB55_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDI6500079.1"
                     /translation="MYEQWKVRQSFVDDVNIITKLFLGIILFFFIIFVHNFDFMLYIT
                     ILMLVFLLMFNGTQFKITAIFITISIGFALLSSLFMILYGNGSHTILKFGFIHITTES
                     LVRGLHVSLRTIAISFFGILIALTSQVVMIFYSLMQHLKVKSKVAYAFMAAIRMIPLM
                     ISSLIQLRRSLKMRYQMIDAANYRGLKRFKHLIIPLLSQNIRKAHQLSVAMESKGFRD
                     GPRTYYYNVPFSYKDIIFIIVLIAIITLAYYCSITLPITGIDDVRLGRVG"
     gene            complement(13951..15351)
                     /locus_tag="POB55_001107"
     CDS             complement(13951..15351)
                     /locus_tag="POB55_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6500080.1"
                     /translation="MLKVSDLRLKYPSGQRKIFDHLNITIQDKEKVLLLGPSGCGKST
                     LLNVLSGIVPNLIELPMKYDELIVDPLSGVIFQDPDSQFCMPKVYEELAFVLENRQVP
                     REDMDALIINALNMVNLNVTPETYIKDLSGGMKQKLAIVETILQQSKTLFLDEPTAML
                     DVQATEDLWTKLIELWEDQTVVIVEHKVKHIWNHVDRVILMDYNGNIIADECPEIILQ
                     KYVHLLSEYGVWHPRAWEFAPSRVDFPTTNSHLLQFKNGRIIRGKSTLLSFSDLEIGL
                     GEWITITGANGSGKTTLLESIMQLIKYQGDVYFENQRLTKIKHAAKHMYLVYQNPELQ
                     FITNSVYDEINIHFNHLSKDQSDDETIQLLKLLDLQNVKDQHPYELSIGQKRRLSVAT
                     ALSSKADIIFLDEPTFGLDSHNTFQLIKLFQKRINLGQSIVMVTHDDEIIERYPSRRL
                     KISDGALLDCDGDTNV"
     gene            complement(15366..15941)
                     /locus_tag="POB55_001108"
     CDS             complement(15366..15941)
                     /locus_tag="POB55_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDI6500081.1"
                     /translation="MSKGLKLSEILVTVLISVVFAVIYNLWWFVYNGVQATGLHLEQL
                     TNGVWFMAAIVCYLIIPKPGIALLAEFAAGAGETIIMGRFDIPTIVYAFIQGLACELV
                     FAIFKYQSRSVMVAMLAGFCTAIAAFPIDYFYGYLNEVAGWNLTLFIVFRLISGAVVA
                     GVLSYLLVKALDKTGVTKLFRPAAKEDYDNL"
     regulatory      complement(16018..16118)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(16525..16659)
                     /gene="graF"
                     /locus_tag="POB55_001109"
     CDS             complement(16525..16659)
                     /gene="graF"
                     /locus_tag="POB55_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance-associated protein GraF"
                     /protein_id="HDI6500082.1"
                     /translation="MSNENQNKKAAEKAKEVEEKLKDKKEEKTEDINQTKQDIQDTLN
                     "
     gene            16811..>17005
                     /locus_tag="POB55_001110"
     CDS             16811..>17005
                     /locus_tag="POB55_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500083.1"
                     /translation="MSFLRKHAEIIFSYLIGIVSLFTGLIILINLPLIKQLNGGKKVD
                     THVHNVWEFLNAFFSEIIKVM"
BASE COUNT         5892 a         2552 c         3223 g         5338 t
ORIGIN      
        1 taatttgaaa tcaaatataa ctttattcga cagtttgaac ttaaattttt gtgtttcaaa
       61 acaataagaa aagggaggcg aaaatgataa agaaccacta atgattaact atgtagtagt
      121 actttatcat agtcacagct tctgtgatgg ggcccccacc ccaactcgca ttgcctgtag
      181 aatttctttt cgaaattctc tgtgttggga cccctcccca acttgcacat tattgtaagc
      241 tgacttttcg tcagcttctt tgttggggcc cacaccccaa ctcacattgc ctgtagaatt
      301 tcttttcgaa attctctgtg ttggggcccc tgactagaat tgaaaacttg ttacaagctt
      361 attttcattc aattaactac tgtctatata aaattgttga gccaagaaca aagtgaaatg
      421 ctcccttcaa agccttcatt tattcaatgt ctactttgaa gggagcattt gatcgattaa
      481 tgtcccagat ttttcttgtt attcatttca attgcgaaat aatattaaat gtgggttaaa
      541 agacttcatt aaaactcagt tattaattct caatacataa ctaatgtaaa tttacgaatc
      601 aatacctcga cgcatttttt ctgcaagcaa agtattattt aataccatag taattgttaa
      661 tggaccaacg ccaccaggaa ctggtgtaat agctccagca atttctttaa ccgcatcata
      721 atcaacgtca ccttttaatt tgccattttc atctggcgta ttgccaacat cgataattac
      781 tgctccttct ttgaccacat cttttgttac taaaccaggc ttaccaactg cactgacaat
      841 gacatcagca tcttttaaat atgatgccat atcttttgaa cgagaatgta agattgttac
      901 tgatgcattt ttttgaagta gtaacttaga aactggttgt ccgacaatat gacttcgtcc
      961 aattacaact gcatttttac cttctaaatc aatatctgca tgttttaata tttccatgat
     1021 gccgagcggt gtgcaaggta caaaagtttg ttcatcgata tataatttcc ctatatttat
     1081 tggatgaaat ccgtccacat ctttttcagg attgattgct tctaatattt tctgttcgct
     1141 aacttgtttt ggtaatggta cttgtaccaa aataccactt acagaatcat cattatttag
     1201 tctatttagt tcgtttaata cttcttcttc agtagctgtt tcttccaaat gtacgatttc
     1261 tgaaatcata ccaatttttt cagctgcttt cttttttgat ctaacataac tttgactagc
     1321 gccatcatta ccaactaata taacggataa tttaggtgta aaaccctttt cttttagcgc
     1381 ttcaacttga tcttgtaacc cctgtctgta gtctttggca atttgtttac catctaaaat
     1441 tttagcaacc ataaaaactt ctcctcctat tgtcaatatc ctaactttgt tagttcataa
     1501 aactaattta catagcaaaa gacgtaaatt caaacaaaaa atcaaaataa agttcgattt
     1561 ttgattgaaa aagcagaaat tgcttgttat gctatatcta taatatacaa ctaaagtaat
     1621 tcacaattca aacttttgaa agggtgtaca aagtgaaagt agcagtcatt atgggcagtt
     1681 cttccgattg gaaaattatg caagagagtt gtaacatgtt ggattatttt gaaattccgt
     1741 acgaaaaaca agtagtatcc gcacatcgta cgccaaaaat gatggttcaa tttgcttctg
     1801 aagcgagaga aagaggtata aacattatca ttgcaggcgc tggcggtgcg gcacatttac
     1861 caggtatggt tgcatcatta acgacgctac cagttattgg tgtgccgatt gaaacaaaaa
     1921 gtttaaaggg tatagattct ttattatcaa ttgttcaaat gccaggaggt attccggttg
     1981 caacgactgc aattggtgca gcaggtgcta aaaacgcagg tatacttgca gcaagaatgt
     2041 taagtattca aaatccttct ttagttgaaa aactaaatca gtatgaatct tcgttaattc
     2101 aaaaagtgga ggacatgcaa aatgagcttc aataaattaa agtttggtgc gactattggc
     2161 attattggtg gtggtcagct tggaaagatg atggcacaat cagctcaaaa aatgggttat
     2221 aaagtggttg tattggatcc ttctgaagat tgtccatgta gatacgttgc acacgaattt
     2281 atacaagcca agtatgacga tgaaaaggca ctcaatcaat taggacaaaa atgtgatgtg
     2341 attacttatg aatttgaaaa catttcagcc caacaattaa aactattatg tgaaaagtac
     2401 aatattccgc aaggttacca agctatacag ttattacaag accgcttaac tgaaaaagaa
     2461 acattaaaaa gtgctggtac caaagttgtc ccgttcattt cagtaaaaga atctacagat
     2521 attgacaaag caattgaaac attaggatat ccttttattg taaaaactag atttggtggc
     2581 tacgatggca aaggtcaagt tttaattaac aacgaaaaag acttacaaga aggttttaaa
     2641 ttaattgaaa ctagtgaatg cgtagctgaa aaatatttga atatcaagaa agaagtatct
     2701 cttactgtta caagaggaaa caacaatcaa atcacttttt tcccattaca agaaaatgag
     2761 catagaaatc aaatactttt caaaacaatt gttccagcga gaatagataa aacagctgag
     2821 gcgaaagagc aagttaataa aattatccaa tcgattcatt tcattggaac atttacagtt
     2881 gaatttttta tagatagtaa caaccaattg tatgtgaacg agatagcacc aaggcctcac
     2941 aattccggac attattcaat tgaagcatgt gattattcac aatttgatac tcatatttta
     3001 gcagttaccg gacaatcatt accaaattca attgaattat taaagccagc agtcatgatg
     3061 aacttactag gtaaagattt agatttattg gaaaatgaat ttaatgaaca tccagagtgg
     3121 cacttacata tttatggtaa gtctgagcgt aaagatagca gaaaaatggg gcatatgact
     3181 gtactaacga atgatgtaaa ccaaactgaa caagatatgt acgctaaatt tgaggggagt
     3241 aattaaagca tgacattatt atatgaagga aaagcgaagc gcattttctc aacaaatcaa
     3301 gaaaatgaat taagagttga atataaagat gaagttactg ctggaaacgg ggctaagaaa
     3361 gacacaatgg caggtaaggg gcgattaaat aatcaaatta cttctattat atttaaatat
     3421 ttacaagaaa atggaataga aagtcacttt attaaacaat tatctgaaac agaacaatta
     3481 gttaagcctg tgaaaataat tccattagaa gtagttgttc gtaatattgc tagtggatct
     3541 attacaaagc gtttaggttt tgaaaatggt gaagttttta gagaaccact tgtagaattt
     3601 ttctataaaa atgatgcgtt aaatgatccg ttgataacgg atgaccatgt taaattgctc
     3661 aatatagcat cagatgaaga tattgaaata ctaaaatcca aagcattaaa gattaataat
     3721 gtgttgaaac aattaatgga tgctatgaat ttaaaattag tagattttaa aatcgaattt
     3781 ggaaagactg agactggtca aattttgtta gcggatgaaa tatctccaga tacatgtcga
     3841 atttgggata aagctaccaa tgcaaacttt gataaagatg tatatagaaa taacactgga
     3901 tcactgattg aaacatatca aatattttta aacaaattgg aggatttaaa ataatgaaaa
     3961 caattgaact acatatcaca ttacaaccac aagtattaga tacgcaagga caaacgctta
     4021 ctcgagctgt acatgactta ggttatgcac aagtgaatga tattcgtgta ggaaaagtat
     4081 tatatatgac agtggatgag gttagtgatg aaaaggtaca caacattatt acaactctaa
     4141 gtgaaaaatt gtttgcaaat acagtgattg aagaatatag ctataaagtg ttagatgatg
     4201 aaaaggagaa tgcataaaat gaaatttgcg gttcttgttt ttccaggttc gaattgtgat
     4261 agagacatgt ttaatgctgc tattaaaagt ggtgttgaag cggaatatgt agattataga
     4321 gaaacatcac taagtggatt tgatggcgta cttattcctg gtggattttc attcggggat
     4381 tacttaagat ctggggcaat ggctagtgta gcgccgatta tttcggaagt taaacgtctt
     4441 gcagctgaag gtaagccagt attaggtgtt tgtaatgggt ttcaaatttt aactgaaata
     4501 ggcttattac ctggtgcatt attgcataac gattcacatt tatttattag tagaaatgaa
     4561 gagttagaaa tagtgaataa tcaaacggca tttacaaatc tttatgaaca aggtgaaaaa
     4621 gttatatatc ctgtagctca cggtgaaggt cattattatt gtactgatga aatatatcaa
     4681 caattaaaag ctaacaatca aattattctg aaatatgtga ataatccgaa cggttcatat
     4741 gatgatattg caggaattgt taacaaaaaa ggcaatgtat gtggcatgat gccacatcct
     4801 gaaagagctt tagaaacgtt gttaggtact gatagtggtg tgaaattatt tgaagcgatg
     4861 gtaaaaagtt ggagggaaca acatgtctaa atttatcgaa ccaagcgttg aagaaattaa
     4921 acttgaaaaa gtatatcaag atatgggatt aagtgatcaa gaatatgaaa aagtttgcga
     4981 tattttaggc agacaaccta actttacaga aacaggtatc ttttctgtta tgtggagtga
     5041 acattgctct tataaacatt ctaaaccgtt tttaaagcaa tttcctacgt caggtgacca
     5101 tgtgcttatg gggcctggtg aaggtgcagg ggtagtcgat ataggtgata atcaagccgt
     5161 agtatttaaa gtagagtctc acaatcatcc atcagcaatt gaaccatatc aaggggctgc
     5221 tacaggcgtt ggtggaatca ttcgtgacat tgtctctatt ggggctagac ctattaattt
     5281 gttaaacagt cttagatttg gagaattaga taataaacaa aaccaaagat tacttaaagg
     5341 tgttgtaaag ggtatcggag gttatggtaa ctgcattggt attccaacaa ctgctggtga
     5401 aatcgaattt gatgaacgtt atgatggcaa tccacttgtt aatgcaatgt gtgttggtgt
     5461 tatcaatcac gacatgattc aaaaaggcac agcaaaaggt gtaggtaatt cggtcattta
     5521 tgttggtttg aaaactggtc gagatggtat tcatggtgct acttttgcat ctgaagaatt
     5581 gacggaagaa agcgaaagta aacgaccttc tgtacaaatc ggtgatccat ttgtaggtaa
     5641 aaaattaatg gaagcaacac ttgaagcaat tacatttgat gaattagttg gtattcaaga
     5701 tatgggtgct gctggtttaa catcttcatc gtctgaaatg gcggcaaaag gtggtagtgg
     5761 gttacatttg agattagaac aagtgccaac acgtgagcca ggtatttctc cttatgaaat
     5821 gatgctttca gaaactcaag aacgtatgtt actagttgtt gaaaaaggta ctgaacaaaa
     5881 attcttagat ttatttgata agcacgaatt ggatagtgct gttataggtg aagttacaga
     5941 tacaaatcgt tttgttttaa catatgatga cgaagtttat gctgacattc cagttgaacc
     6001 actagctgat gaagcacctg tatatatttt agaaggagaa gaaaaagatt ataatacttc
     6061 taaaaatgat tatacacaca tcgatgttaa agatactttc tttaaattac ttaagcatcc
     6121 gactatagca tctaaacact atttatatga tcaatacgac caacaagttg gtgccaatac
     6181 gataattaag ccaggacttc aagcatcggt agtacgtgtg gaaggcacaa ataaggcaat
     6241 tgcttcaaca attgatggtg aagcgcgtta tgtatataac aatccatatg aaggtggaaa
     6301 gatggtagta gctgaagctt atcgaaattt aattgccgtg ggtgcaacac cattagcaat
     6361 gacagattgt ttaaattatg gttctcctga aaagaaagaa atctatcaac agttgataga
     6421 ttcaacgaaa ggtatggcag aagcatgcga cattcttaag acaccagtag tttctggtaa
     6481 tgtatcttta tataacgaaa cgaaaggtac ttctattttc ccaacaccag ttgttggaat
     6541 ggtaggtttg attgaaaatg taaattattt aaatgatttt gaacctcaag ttggagataa
     6601 attatattta atcggtgata ctaaggacga ctttggtggt agtcaacttg aaaagttaat
     6661 ttatggcaaa gttaatcatg aatttgagtc attagatttg agttcagaag ttgaaaaagg
     6721 tgaatcaatc aagaccgcta ttcgtgaagg actattatca catgttcaaa cagttggtaa
     6781 aggtggctta ctgattacct tagctaaact aagtgcgcat tacggtttag gattaaaatc
     6841 ttcaatagat ataacaaatg cacaattgtt tagtgagacg caaggccgat atgttgtttc
     6901 tgttaaatca ggtaaaactt taaatattga taatgcaata gaaattggac ttttaacaga
     6961 tagtgataat ttcaaggtaa caacaccata tacagagatt agtgaaaatg tttcagatat
     7021 taaacaaata tgggaagggg caattgctca atgtttaact actcaggatt aaacgaagaa
     7081 tgtggcgtgt ttggtatttg gaatcatcct gaagcagcgc aactaacata tatgggactt
     7141 catagtttgc aacatcgtgg tcaagaaggt gcaggtatag ttgtttctga tcaaaatgaa
     7201 ttaaaaggcg agcgaggatt aggcttacta actgaagcga ttaaagatga tcaaatggaa
     7261 cgattaaaag gatatcaaca tgcaattggt cacgtccgtt atgctacttc aggtaataaa
     7321 ggtattgaaa atattcaacc gtttctgtat cacttttatg atatgagtgt aggtatttgt
     7381 cataatggta acctcattaa tgctaaatca ttgcgtcaga atttagaaaa acaaggtgct
     7441 atcttccatt cgtcttctga tactgaagtc attatgcatt tgatacgtcg aagtaaagct
     7501 cctacttttg aggaagcgtt gaaagaaagt ttgcgaaaag ttaaaggcgg ttttacattt
     7561 gcgattttaa ctaaagatgc gttatatggc gcagtagatc caaatgctat cagaccactt
     7621 gttgtaggta aaatgaaaga tgggacatac atccttgcaa gtgaaacatg tgcaatagat
     7681 gtgttaggtg cagaatttgt tcaagatatt catgcaggtg aatatgtcgt gattaacgat
     7741 aaaggtatta cagttaaatc ttatacacat catacgacaa ctgcaatttc tgcgatggaa
     7801 tatatttatt ttgctagacc agactcaaca atagctggta aaaatgtcca tgcagtacgt
     7861 aaagcttctg gtaaaaaatt agcccaagaa agccctgtaa atgctgatat ggtcatcggt
     7921 gtacccaatt catcgctatc agctgcgagt ggttatgctg aagaaatagg tttgccatat
     7981 gaaatgggac tagttaaaaa tcaatatgtt gcaagaacat ttattcaacc aactcaagaa
     8041 ttacgtgagc aaggtgtgag agtgaagtta tctgcggtaa aagatatagt agatgggaaa
     8101 aatatcattc ttgttgatga ttccattgtt cgcggtacga caattcgacg cattgtgaaa
     8161 atgttaaaag attctggtgc aaataaagtg catgtgcgta tagcatcacc ggaatttatg
     8221 ttcccaagtt tttatggaat cgatgtttca actacggcag aattaatttc tgcaagcaaa
     8281 tcacctgaag aaattaaaga ttatattggc gctgattcat tagcatatct atctgtagat
     8341 gggttaattg aatcaattgg tttagattat gacgcgccat atagtggctt atgtgtagaa
     8401 agtttcactg gagattatcc tgcagggtta tatgattatg aagcaaatta taaagcacat
     8461 ttaagtcatc gacaaaagca atatatttct aaaaacaaac acttttttga tagcgaggga
     8521 aatttaaatg tctaaagcat atgaacaatc tggtgtaaat attcatgctg gttatgaagc
     8581 tgtagaaaga atgtctagtc atgttaaacg tacgatgcgt aaagaagtta tcggtggttt
     8641 aggtggattc ggtgctacat ttgatttatc acaattaaat atgacagcgc cagttttagt
     8701 ttctggaaca gacggagtag gtacgaaatt aaaactagct atcgactatg ggaaacatga
     8761 ttcgataggt atcgatgcag tcgcaatgtg tgttaatgat attttaacga caggtgcaga
     8821 accattgtat tttttagatt acatcgctac aaataaagtt gttcctgaag ttattgaaca
     8881 aattgttaaa ggtattagtg atgcatgcgt tgaaacgaat actgcactta tcggtggaga
     8941 gactgctgaa atgggtgaaa tgtatcacga aggtgaatat gatgtagccg gatttgcagt
     9001 tggagcagtt gaaaaggatg actatgtaga tagttcagaa gtgaaagagg gacaagttgt
     9061 tatagggctt gcgtcaagtg gcattcattc aaatggatat agtttagtgc gcaaattaat
     9121 taatgaatca ggcattgatt tggcatcaaa cttcgataat cgtccattta tagatgtctt
     9181 tttagaacca actaaattat atgtcaaacc tgtacttgct ttgaaaaaag aagtttctat
     9241 taaggcaatg aatcatatta ctggtggagg tttttatgaa aatattccac gtgcattgcc
     9301 agccggatat gctgctagaa ttgatactac atcatttcca acgccaaaaa tatttgattg
     9361 gttacaacaa caaggcaata tagacacaaa tgaaatgtat aacattttta acatgggtat
     9421 tggctatacg gttatcgttg atgaaaaaga tgcatcacgc gctttgaaga ttttagcaga
     9481 acaaaatgtg gaagcctatc aaattggtca tattatgaaa aatgagtcaa ctgcaattga
     9541 attgttgggg gtataacaat ggttaaaatt gcgatttttg catcaggttc aggaagtaac
     9601 tttgaaaata tagttgagca tgttgaatca ggaaaacttg aaaatattga agttacggcg
     9661 ctatatacgg atcatcaaaa tgcgttttgt atagatagag caaaaaagca cgatattcct
     9721 gtttatatta atgaaccaaa acaatttgat tcaaaagcag cgtatgaaca acatttagta
     9781 tcactattaa atgaagataa ggtagagtgg attattttag ctggctacat gcgtctaatt
     9841 ggtccagatt tattagcttc atttgaaggt aaaatattga atatacatcc atctctattg
     9901 ccgaaatata aggggattga cgcaataggc caagcatatc atagtggcga tactattact
     9961 ggttcgacag tacattatgt tgattgtggt atggatacgg gagaaattat tgaacagaga
    10021 caatgtgata ttagaccgga cgattcaaaa gaacaattag aagagaaagt aaaaaaattg
    10081 gaatatgagt tatatccaag tgttattgct aaaattgtaa aataaggagt atgtatgtga
    10141 tgaagaaagc tattttgagc gtatcaaata aaacaggtat tgtagagttt gctaaagcgt
    10201 taacgcaatt aaattatgaa ttatattcaa caggtggtac taaacgtata ttagatgaag
    10261 caaatgtacc agttcgttct gtttcagact taacacattt tccagaaata atggatggcc
    10321 gtgttaaaac attacatccg gctgttcatg gtggcatttt agctgatcga aataaaccgc
    10381 agcatttaaa tgaattatca gaacaacata tagatttaat tgatatggta gtagttaatt
    10441 tatatccatt ccaacaaact gttgcaaacc ctgatgtgac gatggacgaa gcaattgaaa
    10501 atattgatat tggtggtcca acaatgttac gtgctgcagc taaaaactat aaacatgtaa
    10561 caacaattgt acatccggca gattatcaag aagtattgac gcgattaaga aacgattcgt
    10621 tagatgagtc atatagacaa tcattaatga ttaaagtttt tgagcatact gcagaatatg
    10681 atgaagcgat tgtacgtttc tttaaagggg ataaagaaac tttaagatat ggagaaaatc
    10741 cacaacaatc agcgtatttt gtgagaactt cgaatgctaa gcacacgatt gcaggcgcta
    10801 aacaattaca tgggaaacaa ttaagctata acaatattaa agatgcagat gctacactag
    10861 ctttagttaa aaagtttgat acacctgcta cagttgcggt taaacacatg aatccatgtg
    10921 gtgttggtat cggtgacacg atagaacaag catttcaaca tgcgtatgaa gcggatagtc
    10981 aatcaatatt tggtggaatt gttgcattaa accgagctgt aacacctgag ttagcagagc
    11041 aattgcatag tatctttttg gaagtcatta ttgcaccaaa atttacagat gaagcattag
    11101 atattttaaa acaaaagaag aatgtaagat tattagaaat tgatatgact atagacagta
    11161 acgaagaaga gtttgtttca gtatctggcg gatatttagt tcaagataaa gacaattatg
    11221 tcgtgccaaa agaagaaatg aaagttgtta cagaagtagc acctactgat gaacaatggg
    11281 aagcaatgtt attaggatgg aaagttgtac catcagtaaa aagtaatgca attattttaa
    11341 gtaataataa acaaactgta ggtataggtg ctggacaaat gaatcgtgtc ggtgctgcta
    11401 aaattgcgtt agagagagct attgaaatca atgatcatgt agcgttagta tctgatggat
    11461 ttttccctat gggagataca gttgaacttg cagcacaaca tggtataaag gcaattatcc
    11521 aaccgggtgg ttcgattaaa gatcaagatt caattgatat ggctaataaa catggtattg
    11581 caatggtagt cacaggcact cgacatttta aacactaatt ttaaaggggc gtaacataaa
    11641 tgaatgtatt agtaattggt gctggtggac gagaacatgc acttgcatat aaacttaatc
    11701 aatcgaatct agttaaacaa gtgtttgcca ttccaggtaa tgaggcaatg acacctatag
    11761 ctgaagtaca cactgaaatt tcagaatctg atcatcaagc gatattagat tttgctaaac
    11821 ggcaaaatgt tgattgggta gttataggtc cagaacagcc gctaattgat ggattagcag
    11881 acattttacg agcgaatggt ttcaaagtgt ttggtccaaa taagcaagca gctcaaatcg
    11941 aaggctcaaa attatttgct aaaaagataa tggaaaaata taatattcca actgctgatt
    12001 ataaagaagt tgagcgaaaa aaggatgctt taacatatat tgaaaactgt gaattgcccg
    12061 ttgttgtcaa gaaagatggg ttagctgctg ggaaaggcgt tattattgca gatactattg
    12121 aagcagccag aagtgctatt gagattatgt atggtgatga agaagaaggt actgttgtat
    12181 ttgaaacgtt tttagaaggt gaagagttct cgctaatgac atttgttaat ggtgatttag
    12241 cagtaccttt cgactgtatt gcacaagatc ataaacgcgc atttgatcat gatgaaggac
    12301 caaatactgg tggtatgggg gcttattgtc cagtaccaca tattagtgac gatgttttaa
    12361 aacttacaaa tgaaacaatt gcacaaccca ttgcaaaggc aatgcttaat gaaggttatc
    12421 aattcttcgg tgtattatac attggtgcta ttttaactaa aaatggtcca aaagtaatag
    12481 aatttaatgc ccgttttggt gatcctgaag ctcaagtatt attaagtcgc atggaaagtg
    12541 atttaatgca gcatattatt gatttagatg aaggaaaacg tactgaattc aaatggaaaa
    12601 atgaatctat tgtaggggtc atgttggcat caaaaggata tcctgatgca tatgaaaaag
    12661 ggcataaagt aagtggcttt gatttaaatg aaaactattt tgttagtgga ttaaagaagc
    12721 aaggtgatac ctttgttact tcaggtggta gagttatact tgccatcgga aaaggtgaca
    12781 atgtacaaga tgcacagcga gacgcatacg aaaaagtatc acaaatacaa agtgaccatt
    12841 tattctatcg tcatgacatt gcgaataaag cactacaact taaataagta aatttaaaat
    12901 actaagatta gctatgaacg aatctataac gatagatttt ttcatagctt ttttagttgt
    12961 agagtctagg acattgattt ctgtaccaaa tttgtgatta tgcatatgta atacaaaaga
    13021 ggcgccacaa catgtttgga tgaacaaaat aacatgtttg tggcacctct tttgtttagt
    13081 atggaataaa tggttttctt tttctataca atgaatttct aatttagtat ctatacaatt
    13141 atggataaaa tttaacctac acgaccaaga cgaacatcat ctatgcccgt gatgggtaag
    13201 gtgattgaac aataatatgc caaagtaata atggcaatta aaactataat aaagattata
    13261 tctttatatg agaaaggtac gttgtaatag taagtacgag gaccatctct aaatcctttg
    13321 gactccatcg caactgataa ttgatgtgcc tttctaatat tttggcttaa tagaggtata
    13381 attaaatgct taaatcgctt taaccctcta taatttgctg cgtctatcat ctgatagcgc
    13441 atttttaaag atctgcgaag ctgtattaaa gaactaatca ttaaaggtat catacgaatg
    13501 gcagccatga atgcataagc aacttttgat ttaaccttta aatgttgcat taaactataa
    13561 aatatcatga caacctgaga tgtaagtgcg attaagatac cgaaaaatga aatagcaatg
    13621 gttcttaatg atacatgtaa accacgaact aaactttctg ttgtaatatg gataaatccg
    13681 aatttcaaaa ttgtatggct accattcccg tataaaatca tgaacaggga agagagtaat
    13741 gcaaagccaa tacttatagt tataaaaatt gctgtaattt taaactgagt accattaaac
    13801 atcaataaga aaactaacat taagatagtg atataaagca taaaatcgaa attatgcaca
    13861 aatataataa agaaaaatag tataattcca agaaatagtt tcgttataat gttgacatca
    13921 tcaacaaatg attgccgaac tttccattgc tcatacattc gtatcaccat cacaatctag
    13981 taacgcacca tctgaaattt taagtcttct tgatggataa cgttcaatta tttcatcgtc
    14041 atgtgtaacc atgacaatac tttgtcccaa attaattcgc ttttggaaaa gtttgatcaa
    14101 ctggaatgta ttatggctat caagtccaaa tgtcggttca tctaaaaaga taatatcagc
    14161 tttagaactt agtgcggtag ctacgctaag gcgtcgtttt tgaccaatag acaactcata
    14221 aggatgttga tcttttacat tttgtaaatc taaaagtttt aaaagttgta tcgtttcatc
    14281 atcactttga tctttagaaa ggtgattaaa atgaatgtta atttcatcat aaaccgaatt
    14341 tgttataaat tgtaattctg ggttttgata aactaggtac atgtgttttg ctgcatgttt
    14401 aatttttgtt aaacgctgat tttcaaaata aacatcacct tgatatttaa tcaattgcat
    14461 aattgattca agcaaggttg ttttaccact accatttgcc cctgtaattg taatccactc
    14521 acctagacca atttctaaat ctgagaatga gagcaatgtt gatttaccgc gaataatacg
    14581 tccattttta aattgtaata agtgtgagtt tgttgttgga aagtcaacac gacttggtgc
    14641 gaattcccat gcacgtggat gccacacacc atattcactg agtaaatgaa catacttctg
    14701 taatatgatt tcaggacatt catcggcaat gatatttccg ttataatcca tcaaaatgac
    14761 gcggtcgaca tgattccaga tgtgtttaac tttatgttca acgattacaa cagtttgatc
    14821 ttcccaaagt tcaattagtt tagtccataa atcttctgtt gcttgaacat ctaacattgc
    14881 tgtcggttca tctaaaaaca atgtttttga ttgttgaaga atggtttcaa caattgccaa
    14941 tttctgtttc atcccgccac ttaaatcttt gatatacgtt tcaggggtaa catttaaatt
    15001 gaccatattt aaagcattga taattaacgc atccatgtct tcacgtggta cttgtctatt
    15061 ttctaaaacg aatgcaagtt cttcgtatac ttttggcata caaaactggc tatcagggtc
    15121 ttggaaaata acgccactta atgggtcaac gattagttca tcatatttca taggtaattc
    15181 aattaaatta ggaacaatac cacttaatac attcagaagt gtacttttac cgcaaccaga
    15241 aggaccgagt aaaagtactt tttctttgtc ttgaatagtg atatttaaat gatcgaaaat
    15301 tttacgctga ccacttggat attttaatcg taaatcactt acttttaaca ctctgaatgc
    15361 tcctcttata agttgtcgta atcttcttta gcagctggtc taaataattt tgttacgcct
    15421 gtcttatcta aagcttttac taaaaggtaa gataggacgc cggcgactac tgcaccacta
    15481 attaatctaa atacgatgaa taatgttaag ttccaacctg caacttcatt taaataacca
    15541 tagaaataat ctatagggaa agccgcgatt gctgtacaaa aacctgctaa catagctacc
    15601 ataactgaac gagattgata tttaaaaatt gcaaagacaa gttcacacgc taaaccttgt
    15661 atgaaagcgt aaacgattgt cggaatatcg aaacgaccca taatgatagt ttcgccggca
    15721 cctgcagcaa attcagccag taaagcaata cctggttttg gaataattag atagcagaca
    15781 atcgctgcca tgaaccaaac cccgtttgtt aattgttcga ggtgaaggcc tgtagcttgc
    15841 acaccattgt aaacaaacca ccataaattg taaataactg cgaatactac tgaaataagt
    15901 acggttacta gtatttcaga tagctttaaa ccttttgaca tttttacatc ctcctaataa
    15961 aaaaacgcac aaccatccat aggaaagtta tgcgttcaca atatatatta gtaaaacata
    16021 tgtatagtaa cactttccta cgctagttca agctagatca ggttcaaagg gtttgagggc
    16081 aagcctcatc tcagtataaa acacccctag tgtgtgcgat ttatttaatt aattatactg
    16141 taagacgttt gtaaacttat gtcaataggt tgtcttcatg aaattttgtt taattcgatt
    16201 taaaatttat aatattagca ttggatttaa attgaagatg tagtaggaat gttagtaatt
    16261 aaagatataa aaatatgtga catgtaataa gattgagctg ataaatgaag agggatactt
    16321 atcaatcata cctctttaac aacagtgaag aacccgtgca taatggctta cgaattatag
    16381 tttgtagggt aaaagaggga tacatgcgcc gagcacatgc aaaaaagccc ctaacaacta
    16441 aaagttgtaa ggaagaagag ggacacatgc gccgagcaca tgcataaaat cccctaacaa
    16501 ctaaaagttg taaggggatt taaattaatt tagtgtatct tggatatctt gttttgtttg
    16561 attaatatct tctgtttttt cttctttttt atcttttaat ttttcttcaa cttctttagc
    16621 tttttctgct gcttttttat tttgattttc attagacatg attaattcct cccaaattgg
    16681 ataattattt atatataaat cttacccggt tgtactttcg ttaaactttt ctaagtctat
    16741 agcactattt attcatttat ctaaagacaa caacattaga ttaatatata atgattttga
    16801 ggtgaacata atgtcttttc ttaggaaaca cgccgaaatt atttttagct atttaatcgg
    16861 tatcgtttca ctcttcactg gtctcattat tttaattaac ttgccattaa ttaaacaatt
    16921 aaatggtggt aaaaaagttg atacacatgt tcataatgtg tgggaatttc tgaatgcatt
    16981 tttcagtgaa attattaaag taatg
//
LOCUS       DAMZKC010000045        16807 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.045, whole genome shotgun sequence.
ACCESSION   DAMZKC010000045 DAMZKC010000000
VERSION     DAMZKC010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 16807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16807
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.045"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            48..812
                     /locus_tag="POB55_001111"
     CDS             48..812
                     /locus_tag="POB55_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001798951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDI6500084.1"
                     /translation="MKRLKKIVLCISFLFLTIFIGGCGMGKEAEIKKSFEKTLSMYPI
                     KNLEDLYDKEGYRDDQFDKKDKGTWIINSEMATQNKGEALKIKGMVLYMNRNTKTTKG
                     YYYVNAIKNDKDGRPQENEKRYPVKMIDNKVIPTKEIKDENIKTEIKNFKFFVQYGNF
                     KDLKNYKDGDISYNPEAPIYSAKYQLTNDDYNVKQLRERYDIPTNKAPKLLLKGSGNL
                     KGSSVGYKNIEFTFVEEKGKNIYFSDSLDYKKSGEV"
     gene            815..2191
                     /locus_tag="POB55_001112"
     CDS             815..2191
                     /locus_tag="POB55_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500085.1"
                     /translation="MAKAEYEIDPGKITSNSEETSAISNISYEIENANDNNLDNEKIR
                     GQITKLKKGGNFPKNLDYIDSYTDPSTGTTATAFLNKDTGKVTVGMAGTNFHGDQLKR
                     VALSSMSPLLFPPSKQDMKDVRGTMKDGVADLAIGVGMVNYKGKHFANTQQFIENLQK
                     KYEIDTVTGHSLGGRDAIFLGLRYNIKNVVAYNPAPLEVKSIRNKFGGQLFRNTTFPD
                     EKYLKELMDNYDGDITKVITQKDGLDYLVKRTDHLTCGDVLRINNGQGHAMENFLGEK
                     EQREIIEELMLVKGYRDANDKAFKALKKNTEKKLGKIDEIKSKLLQTNGGALSSSQQK
                     LLETLVAFSVAEGLSKMVDQELQQLKNMFNLMDEKFEANWKDAQEASDIVGKHLSYPE
                     KVSALDNGGVNESKLATEPHNEIKDKLNKITDLSKKYNSYLQQIEKSINEIVAKDQQL
                     AGQIGDLI"
     gene            2191..2517
                     /locus_tag="POB55_001113"
     CDS             2191..2517
                     /locus_tag="POB55_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500086.1"
                     /translation="MFEEYKKIDDLENAYEIELKRIEREIQNLSDLKYHLRRENEQSY
                     DAFLYLKNKMNYSEESNAKVRRLVEEFDYEADTYIRQKELKLEDYKEEIRREYIQQSE
                     KIMEAK"
     gene            2521..2832
                     /locus_tag="POB55_001114"
     CDS             2521..2832
                     /locus_tag="POB55_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000512632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500087.1"
                     /translation="MGIPDPLSNPMSDPLLGDSWMVGDLKKRQEKKLITTVIAPEHDE
                     KHRENKSKMKKSEKQLINQLINHGHTFKSDFKNVAKGDWVNKAMQDLDKLGNELKKIM
                     N"
     gene            3084..5321
                     /locus_tag="POB55_001115"
     CDS             3084..5321
                     /locus_tag="POB55_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020808042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI6500088.1"
                     /translation="MQRDYLIRVETESMPDFKRLNGLMIGFVIKGEAHIYDENNMTQC
                     NSGDIFIINHRDLYRFQLQQDGIICYIQFQMKYLADKFDDAHCLYFHLTDATTTKNIH
                     QLRNIMARLVSTHIRHNELSKLTEQQLVIQLLMHMIHYVPRTYHSNQSILNDDKVNQV
                     CDYIELHFHEDLSLSELSEYVGWSESHLSKKFTESLGVGFQHFLNTTRIEHAKLDLTY
                     TDETITDIALQNGFSSAASFARTFKHFTHQTPKQYRGDRPAITENQQSAQHNYHDREL
                     ILLLNDYIEEMNHFIEDIEKMNYKEIAFKPTNQQLNQFNHIIQVGYLRNLLNTQYQSQ
                     LLTCHHDFQVNEVLAYDVMPYIMKKLNAPFTYDAEISNIFYDIDLCLDFLLDHNFSLT
                     MHLNQYDSRDYIDAFKVFIHHVALHVSHRKDLKFNLYVTTLHNALIEMIDYFKALFPN
                     GGLYIHLDQATERHLPLLKRLEPHIDHFVFDANSNDAVDFNKMNDDEFKTASQMIINK
                     TNYFIDLIHRHNLKRPLILLNWNTLTGDTFITNGEYFRGGIIIEQLLKLSSKVEGIGY
                     WLNYDLHVSHCKNERDYMNSIELFHQYNGKRPVYFTALLFNKLTSNILYSDDTCIVTG
                     TDSNFQILLYDAKHFNPYLALDNQMNMRATEMIHLNINALEEGMYKIKHFTLDKENGA
                     LFNLWRKHHTIHGMDKDSIDYVNRMSFPKLEVYDIDITDTLALNIKMITNGIHLIEVK
                     RYPSS"
     gene            5472..6650
                     /locus_tag="POB55_001116"
     CDS             5472..6650
                     /locus_tag="POB55_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDI6500089.1"
                     /translation="MNQQLIETLKSKEGKMIEIRRYLHQHPELSFHEDETAKYIAEFY
                     KGKDVEVETNIGPRGIKVTIDSGKPGKTLAIRADFDALPITEDTGLSFASQNKGVMHA
                     CGHDAHTAYMLVLAETLAEMKDSFTGKVVVIHQPAEEVPPGGAKAMIENGVLDGVDHV
                     LGVHVMSTMKTGNVYYRPGYVQTGRAFFKLKVQGKGGHGSSPHMANDAIVAGSYFVTA
                     LQTVVSRRLSPFETGVVTIGSFDGKGQFNVIKDVVEIEGDVRGLTDATKATIETEIKR
                     LSKGLEALYGVTCTLEYNDDYPALYNDPEFTEYVAKTLKEANLDFGVEICEPQPPSED
                     FAYYAKERPSAFIYTGAAVEDGEIYPHHHPKFNISEKSLLISAEAVGTVVLDYLKGDN
                     "
     gene            6652..8040
                     /locus_tag="POB55_001117"
     CDS             6652..8040
                     /locus_tag="POB55_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6500090.1"
                     /translation="MNETYRGGNKLILGIVLGVITFWLFAQSLVNVVPNLQQSFGADM
                     GTISIAVSLTALFSGMFVVGAGGLADKIGRVKMTNIGLLLSIIGSALIIITNLPALLI
                     LGRIIQGVSAACIMPSTLAIMKTYYEGAERQRALSYWSIGSWGGSGICSLFGGAVATT
                     MGWRWIFIFSIIVAVLSMLLIKGTPETKSEVTNTHKFDVAGLIVLVVMLLSLNVVITK
                     GAALGYTSLWFFGLIAIVIVAFFIFLKVEKKVDNPLIDFKLFENKPYTGATISNFLLN
                     GVAGTLIVANTFVQQGLGYTALQAGYLSITYLIMVLLMIRVGEKLLQKMGSKRPMLLG
                     TFIVVIGIALISLVFLPGIFYVISCVVGYLCFGLGLGIYATPSTDTAISNAPLDKVGV
                     ASGIYKMASSLGGAFGVAISGAVYAGAVAATSIHTGAMIALWVNVLMGIMAFIAILFA
                     IPNDDKRVKDAK"
     gene            complement(8527..10188)
                     /locus_tag="POB55_001118"
     CDS             complement(8527..10188)
                     /locus_tag="POB55_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HDI6500091.1"
                     /translation="MSVTEVIFSFLGGLGIFLYGLKIMGDGLQASAGDRLRDILNKFT
                     SNPVLGVIAGIVVTILIQSSSGTTVITIGLVTAGFMTLKQAIGVIMGANIGTTVTAFI
                     IGIDLGEYAMPILALGAFLIFFFKRSKINNIGRILFGFGSLFFGLEFMGDAVKPLASL
                     DGFKQLMLDMSSNPILAVIVGAGLTALVQSSSATIGILQEFYQQDLISLNAAIPVLLG
                     DNIGTTITAILASLAGSIAAKRAALVHVIFNLIGVIIFTIFLPVVIHLISLLQDVWHL
                     KPAMTIAVSHGIFNITNTLIQLPFVAGLAWIVTKLVPGKDIADDYKPQHLNKDLVYHA
                     PGVALQETQKELQNVGQIVLSMFEDIREITKDDKKLIKKLEQKHQAVETINDSIRNYL
                     VRISTKAITKADVERLAVMFDVNRSILKVAELTEEYVAQLKRQHDEDIRITEDAQRGM
                     DKLFNHVAESFDKAIDMLDVYDKTKKDEIVERSRESFNIEHKLRKGHIKRLNRGECTT
                     KGGLLYIDMIGVLERIGYHSRNVSEALVGLNDDVPTDEEIATTEI"
     gene            complement(10512..12287)
                     /locus_tag="POB55_001119"
     CDS             complement(10512..12287)
                     /locus_tag="POB55_001119"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HDI6500092.1"
                     /translation="MYYSYGNYEAFARPKKPENVENKSAYLIGSGLASLAAACFLIRD
                     GQMEGSKIHILEELPKAGGSLDGENMPLKGYVVRGGREMENHFECLWDLFRSIPSLEI
                     DNASVLDEFYWLNKEDPNYSRCRVIEKQGQRLVTDGDFTLTKKAIKEILDLCLTNEED
                     LDDVKITDVFSDDFFNSNFWIYWKTMFAFEPWHSAMEMRRYLMRFVHHIGGLADFSAL
                     KFTKYNQYESLVLPMVEYLKSHGVQFEYGVKVEDIKVDVTTSQKIAREILIHRHGKAE
                     SIKLTVDDLVFVTNGSITESSTYGDNDTPAPPTDELGGSWTLWKNLARQSPEFGNPDK
                     FCQNIPQKSWFVSATSTTNNKDIIDTIESICKRDPLAGKTVTGGIITINDSAWQISFT
                     INRQQQFKDQPKNEISTWIYALYSDVNGDYIKKPITECSGNEICQEWLYHLGVPTDKI
                     EDLAKHASNTIPVYMPYITSYFMTRAIGDRPLVVPHQSQNLAFIGNFAETERDTVFTT
                     EYSVRTAMEAVYQLLNIDRGIPEVINSTFDLRVLMDAIYELNDHQDLREITKDSKMQK
                     LALAGFLKKIKGTYIESLLKEHKLL"
     gene            12638..13111
                     /locus_tag="POB55_001120"
     CDS             12638..13111
                     /locus_tag="POB55_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HDI6500093.1"
                     /translation="MSKIRSFTILSLLIYLAMMCYTVVTYSKLPTKVPIHYNLAGDAD
                     NFADKWVLLLINSAFIVIWLIFFIAGRYYERFAKWSHYNHTPREIRAIKLFLSTLNLE
                     IMSYMSIFTVLEIWQIQHHHQFNLLWFNMIFIIIIGLTLVIFCLLPTIHKMRDSQ"
     gene            13373..14965
                     /locus_tag="POB55_001121"
     CDS             13373..14965
                     /locus_tag="POB55_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HDI6500094.1"
                     /translation="MTLLTVNPFDNVGLSALVAAVPIILFLLCLTVFKMKGIYAALTT
                     LVVTLIVALFVFELPVRVSAGAITEGVVAGIFPIGYIVLMAVWLYKVSIKTGQFSIIQ
                     DSIASISEDQRIQLLLIGFCFNAFLEGAAGFGVPIAICAVLLIQLGFEPLKAAMLCLI
                     ANGAAGAFGAIGLPVSIIDTFNLSGGVTTLDVARYSALTLPILNFIIPFVLVFVIDGM
                     KGIKEILPVILTVSGTYTGLQLLLTIFHGPELADIIPSLATMVVLAFVCRKFKPKNIF
                     RLKESEHKIQKRTPKEIVFAWSPFVILTAFVLVWSAPFFKKLFQPGGALESLVIKLPI
                     PNTVSDLSPKGIALRLDLIGATGTAILLTVIITILITKLKWKSAGALLVEAIKELWLP
                     ILTISAILAIAKVMTYGGLTVAIGQGIAKAGAIFPLFSPVLGWIGVFMTGSVVNNNTL
                     FAPIQATVAQQISTSGSLLVAANTAGGVAAKLISPQSIAIATAAVKKVGEESALLKMT
                     LKYSIIFVAFICVWTFILTLIF"
     gene            complement(15294..16748)
                     /gene="spa"
                     /locus_tag="POB55_001122"
     CDS             complement(15294..16748)
                     /gene="spa"
                     /locus_tag="POB55_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal protein A"
                     /protein_id="HDI6500095.1"
                     /translation="MKKKNIYSIRKLGVGIASVTLGTLLISGGVTPAANAAQHDEAQQ
                     NAFYQVLNMPNLNADQRNGFIQSLKDDPSQSANVLGEAQKLNDSQAPKADAQQNNFNK
                     DQQSAFYEILNMPNLNEAQRNGFIQSLKDDPSQSTNVLGEAKKLNESQAPKADNNFNK
                     EQQNAFYEILNMPNLNEEQRNGFIQSLKDDPSQSANLLSEAKKLNESQAPKADNKFNK
                     EQQNAFYEILHLPNLNEEQRNGFIQSLKDDPSQSANLLAEAKKLNDAQAPKADNKFNK
                     EQQNAFYEILHLPNLTEEQRNGFIQSLKDDPSVSKEILAEAKKLNDAQAPKEEDNNKP
                     GKEDGNKPGKEDNNKPGKEDGNKPGKEDNKKPGKEDGNKPGKEDNNKPGKEDGNGVHV
                     VKPGDTVNDIAKANGTTADKIAADNKLADKNMIKPGQELVVDKKQPANHADANKAQAL
                     PETGEENPFIGTTVFGGLSLALGAALLAGRRREL"
BASE COUNT         5586 a         2586 c         3093 g         5542 t
ORIGIN      
        1 gtaattcaca aaagcaaatc atataaaaag aagagagttg tgatacggtg aagcgtttaa
       61 aaaaaatagt tttatgtatt agttttctat ttttaacaat ttttattggg ggttgtggca
      121 tgggtaaaga agcggaaatt aaaaaaagtt ttgaaaaaac gttgagtatg tatccgatta
      181 aaaatctaga agatttatac gataaagaag gatatcgtga tgatcaattt gataaaaaag
      241 ataaaggcac atggattatt aattctgaaa tggcgactca aaataaggga gaagctttga
      301 aaattaaagg tatggtctta tacatgaata gaaatacaaa aacaacaaaa ggatattatt
      361 atgtaaatgc aataaagaat gataaagacg gaagacccca agagaatgaa aaaaggtacc
      421 cagttaaaat gattgataat aaagttattc caacaaaaga aattaaagat gaaaacataa
      481 aaacagaaat caaaaacttt aagttctttg ttcaatatgg caactttaaa gatttgaaaa
      541 attataaaga cggagatatt tcatataatc cagaagcccc gatttattca gcgaaatatc
      601 aactaactaa tgatgattat aatgtaaaac aattacgtga aagatatgat ataccgacga
      661 ataaagcgcc aaagttattg ttaaaagggt cagggaactt aaaaggttca tcagttggat
      721 ataaaaatat agaatttact tttgtagagg aaaaaggtaa aaatatatac tttagtgata
      781 gcttagatta taaaaaaagt ggagaagtat aattatggct aaagcagaat atgaaattga
      841 tcctggaaaa ataacaagta attcggaaga aactagcgca atatctaata ttagttatga
      901 aatagaaaat gcaaatgata ataatttaga taatgaaaaa attagagggc aaattactaa
      961 attaaaaaag ggtgggaatt ttcctaaaaa cttagactac atagatagtt atacagaccc
     1021 cagtacgggg accacggcaa cggcattttt aaataaagat acaggtaaag ttactgttgg
     1081 aatggctggt acaaactttc acggcgatca acttaaaaga gtagcactta gttcgatgtc
     1141 tccgttatta ttcccaccct ctaagcaaga tatgaaagat gtacgaggca ctatgaagga
     1201 tggtgtagcg gatttagcca ttggagtagg aatggtcaat tataaaggaa aacattttgc
     1261 aaatacacag cagtttattg agaatttaca aaaaaagtac gaaattgata ccgttactgg
     1321 tcattcttta ggagggagag atgctatctt tcttgggctt cgttataaca tcaaaaatgt
     1381 tgtggcatac aatcctgctc cgttagaagt aaaaagcatt cgtaataaat ttggtggtca
     1441 attgtttaga aatacgacat ttccagatga aaaatattta aaagagttaa tggataatta
     1501 tgatggagat attactaagg ttataactca aaaggatgga ttagactatt tagtgaagcg
     1561 tacagatcat ttaacttgtg gtgatgtgtt acgtataaac aatggtcagg gccatgcgat
     1621 ggagaatttt ttaggagaaa aagaacaacg tgaaataatc gaagaactaa tgttagtaaa
     1681 aggatatcgc gatgcaaatg acaaagcatt taaggctttg aaaaaaaata ctgagaaaaa
     1741 actcggtaaa atagatgaaa tcaaatcgaa gttactgcaa acgaacggtg gcgccctctc
     1801 gtcttctcaa caaaaattgc ttgaaacttt agtggcattt tcggtagctg aaggactaag
     1861 taaaatggtt gaccaagaac ttcagcagtt gaaaaatatg ttcaatctta tggatgagaa
     1921 atttgaagca aattggaaag acgcacaaga agctagtgat attgtaggta aacatctttc
     1981 atacccagaa aaggtaagtg cattagataa cggtggtgtc aatgaaagta aacttgctac
     2041 cgaaccacat aatgaaataa aagacaaact aaataaaatt acggacttat ctaagaaata
     2101 taattcgtat ttacagcaaa tcgaaaaaag tattaatgaa atagttgcta aggatcaaca
     2161 attggcaggg caaataggtg atttaatatg atgtttgaag aatacaagaa aatagatgat
     2221 cttgaaaatg catatgaaat agagttgaaa cgtattgaaa gggagataca aaatcttagt
     2281 gatttaaaat atcaccttag aagagaaaat gaacaaagtt atgatgcatt tttgtattta
     2341 aagaataaaa tgaactatag tgaagagtca aatgctaagg tgagacgact agttgaagaa
     2401 tttgattatg aagcagatac ttatattaga caaaaagagt taaagttaga agattataaa
     2461 gaagaaataa gaagagaata tatacagcaa tctgaaaaaa taatggaggc gaagtagctt
     2521 atgggaatcc ctgatccgtt atctaatcct atgtctgacc ctttactagg agatagttgg
     2581 atggtaggtg atttgaaaaa aagacaggaa aaaaaactaa tcacaacagt catcgcacct
     2641 gaacatgatg aaaagcatcg tgaaaataaa agtaaaatga aaaaatctga aaagcaattg
     2701 atcaatcagt taattaatca tggccataca tttaagagtg attttaaaaa tgtagctaaa
     2761 ggtgattggg ttaataaagc catgcaagac ttagataaat taggcaatga gcttaaaaaa
     2821 ataatgaatt agttgaattg tcacatactt tttcaaacca acaacgaact aaaaaatcca
     2881 tataaatatg attaaaagaa gtcataacca ttcgtaaagg atggttattt ttaatagtaa
     2941 acaaaattgc tgctaaccta catcttgaac caagtgagga taaataacct cctgcctccc
     3001 acacgattca acacgaattt caaatatatt gctgtaaaat aatgtaaaat aaacgtattc
     3061 atattactga attgagggat agtatgcaac gtgattattt aattcgagta gaaactgaaa
     3121 gcatgccaga tttcaaaagg ctcaatggtt taatgattgg ttttgttatt aaaggtgagg
     3181 cacatattta tgatgaaaat aacatgacgc aatgcaacag tggcgacatt ttcatcatta
     3241 accaccgtga cttgtatcga tttcaacttc aacaagatgg catcatatgt tatatccaat
     3301 tccaaatgaa atatttggca gacaagtttg atgatgcgca ttgtctatat tttcacttaa
     3361 cagatgcgac cacaaccaag aatatacatc aactgagaaa tataatggca agactggttt
     3421 caacacacat tcgacataat gagttgtcta aattgactga gcaacaactt gtgattcagt
     3481 tgcttatgca tatgattcat tatgtcccgc gtacatatca ttcgaaccaa agtatcttaa
     3541 atgatgataa agtgaatcaa gtatgcgact atatcgagtt acattttcat gaagatttaa
     3601 gcctttcaga attaagcgaa tacgttgggt ggtcagagag ccatctgtct aaaaagttta
     3661 cagaatcgct aggtgtagga ttccaacatt tcttaaatac gacgcgaatt gagcatgcga
     3721 aactcgattt aacatacaca gatgaaacga ttactgatat tgcattgcaa aatggctttt
     3781 caagtgcagc gagctttgcg agaacattta aacactttac gcatcaaacg cctaaacaat
     3841 atcgaggtga tcgtccagca atcactgaaa atcaacaatc ggcacaacat aattatcacg
     3901 accgtgaatt gatattactt ttaaatgact acattgaaga aatgaatcat ttcattgaag
     3961 atattgaaaa gatgaactat aaagagattg cctttaaacc aactaatcaa caactaaatc
     4021 aatttaatca tattattcaa gtgggctatt tgaggaattt gctcaataca cagtatcaat
     4081 cacagttgct tacatgtcat catgattttc aagtcaatga agtattagca tatgatgtga
     4141 tgccatatat tatgaaaaag ctcaatgcgc cattcacgta tgatgcagag atttcgaata
     4201 tattttatga tatcgatttg tgtttagact ttttattaga tcataatttt agtctaacca
     4261 tgcatttgaa tcagtatgac tcacgagatt atatcgatgc attcaaagta tttatccatc
     4321 acgttgccct gcatgtcagt catagaaaag atttgaagtt caacttgtat gtgacgacat
     4381 tgcacaatgc tttgattgaa atgattgatt attttaaagc gttattccct aatggtggct
     4441 tgtacattca cttagatcaa gctacggaaa gacatctacc attgttgaaa cgacttgagc
     4501 cacacatcga ccattttgta tttgatgcca attcaaatga tgctgttgat tttaataaaa
     4561 tgaatgatga tgaatttaaa accgcgagtc aaatgattat taataaaacg aattacttta
     4621 tcgacttaat acatcgtcat aacctaaagc gtccactcat tttactcaat tggaatacat
     4681 tgacgggtga tacatttata accaacggcg aatattttag aggtggtatc atcattgagc
     4741 agttattaaa gttaagctct aaagtagagg gtatcgggta ttggttgaat tatgatttgc
     4801 acgttagtca ttgtaaaaat gaacgggatt atatgaattc tattgaactg tttcatcaat
     4861 ataatggaaa acgtccggtc tatttcacgg cattgctatt taataaatta acaagcaata
     4921 ttttgtattc tgatgataca tgtattgtca cgggaactga ttcaaatttt caaatattgt
     4981 tatatgatgc aaagcatttt aatccgtact tagcgttgga caatcaaatg aatatgcgtg
     5041 caacggaaat gatccatttg aacattaatg ccttggaaga aggtatgtat aagattaaac
     5101 attttacctt agataaagaa aatggtgcgt tatttaatct ttggcgcaaa catcatacga
     5161 tacatggcat ggacaaggac tctatagatt acgttaatcg aatgagtttt ccgaaattag
     5221 aagtatatga tatagatatc acggacacac tggcattaaa cattaaaatg attacgaatg
     5281 ggattcactt aattgaagta aaacgttacc caagttcata aaatgatcac aaatcacaaa
     5341 ttttgatata cataatttgt gattttttat attcaaagct aaaattgcaa aaaattaatg
     5401 gttaacatct ctgttgttgg caatataaat aatgattaat catttatgat gtaactaagg
     5461 agatgaagga tatgaatcaa caattaattg aaactttaaa atctaaagaa ggcaaaatga
     5521 ttgagatcag acgttattta catcagcatc cagaattatc ttttcatgaa gatgaaacgg
     5581 cgaaatacat cgctgaattt tacaaaggta aagatgtgga agtagaaacg aatatcggac
     5641 cacgtggaat taaagtaacg attgattcag ggaaacctgg taaaacatta gcaatccgtg
     5701 cagactttga tgcattacct attactgaag atacaggatt atcttttgca tcacaaaata
     5761 aaggtgttat gcacgcatgt ggtcacgatg cacatacagc atacatgctt gtattagcag
     5821 agacgcttgc tgaaatgaaa gatagtttta caggaaaagt cgttgtgata catcaaccag
     5881 ctgaagaagt accaccaggt ggtgctaaag caatgattga aaatggtgta ttagacggtg
     5941 ttgatcatgt attaggtgta cacgtcatga gcacaatgaa aacaggtaat gtgtattaca
     6001 gacctggtta tgttcaaaca ggacgcgcat ttttcaaatt gaaagttcaa ggtaaaggtg
     6061 gtcatggttc atcaccccat atggccaatg atgccattgt tgcaggtagc tacttcgtca
     6121 cagcgttaca aacagttgta tctagacgac taagtccatt tgaaaccggt gttgtcacaa
     6181 tcggttcatt tgacggtaaa ggtcaattca atgtcattaa agatgttgtt gaaattgaag
     6241 gtgatgtacg tggattaaca gatgctacaa aagcaacaat tgaaacagaa attaaacgtt
     6301 tatcaaaagg attagaagca ttgtatggtg taacttgcac attagaatat aacgatgatt
     6361 atcctgcatt atataatgat ccagagttta ctgagtacgt ggctaagacg ttaaaagaag
     6421 caaaccttga ttttggtgtc gaaatatgtg aaccacaacc accttcagaa gactttgcat
     6481 actatgctaa agaacgtcca agtgccttta tttatacagg tgcagctgtg gaagatggtg
     6541 aaatttaccc acatcatcat cctaaattta acatttcaga aaaatcatta cttatttcgg
     6601 cagaagctgt agggacagtt gttttagatt accttaaagg agataactaa catgaatgaa
     6661 acgtatcgcg ggggcaacaa gttaatctta ggtattgtat taggtgttat tacattttgg
     6721 ttgtttgcac aatcacttgt aaatgttgta ccaaatttac aacaaagttt tggtgcagac
     6781 atgggaacaa ttagtattgc ggtaagtcta accgcactat tttcaggcat gtttgttgtt
     6841 ggagcaggcg gcctagcaga taaaattggg cgcgtgaaaa tgacgaatat cggtttattg
     6901 ttaagtatta ttggttcagc attaattatt attacgaatt taccggcatt attaatttta
     6961 ggtcgtatta tacaaggcgt atcagcagcg tgtattatgc cttccacatt ggccattatg
     7021 aaaacttatt atgagggtgc tgaacgtcag cgcgccttaa gctactggtc tatcggttct
     7081 tggggtggaa gtggtatctg ttcactcttc ggtggggcag ttgcgacaac tatgggttgg
     7141 agatggattt tcatcttctc aattatcgtt gccgtacttt caatgttact catcaaaggg
     7201 acgcctgaaa cgaaatcaga agttaccaat acacataaat ttgacgttgc agggctaatt
     7261 gttctagtag ttatgttgct aagtttaaac gttgtcatta ctaaaggtgc agcacttggt
     7321 tacacatcat tatggttctt tggtttaatt gcaatcgtaa ttgtagcatt ctttattttc
     7381 ttaaaagttg agaaaaaagt agataatccg cttattgatt ttaaattatt tgaaaataaa
     7441 ccatatacag gtgcaacgat ttcgaacttc ttattaaacg gtgttgcagg tacattaatt
     7501 gtagcgaata cattcgtgca acaaggttta ggttatacag cattgcaggc aggatactta
     7561 tcaattactt atttaatcat ggtgttattg atgattcgag ttggtgaaaa attattacaa
     7621 aaaatgggtt ctaagcgacc aatgttatta ggtacattca ttgtggtcat tggtattgca
     7681 cttatttcat tagtattctt accaggcata ttttatgtta ttagttgtgt cgtaggatat
     7741 ttatgtttcg gactaggctt aggtatttat gcaacacctt ctacagatac agctatttcg
     7801 aatgcaccgt tagataaagt tggcgttgct tcaggtattt ataaaatggc ttcatcactt
     7861 ggtggtgcat tcggtgtcgc aattagtggt gcagtatatg ccggtgcagt tgctgcaact
     7921 agcattcata caggtgcgat gattgcactt tgggttaacg tattaatggg aatcatggca
     7981 tttatcgcaa ttttattcgc gattcctaat gatgataaac gtgtcaaaga tgcgaaataa
     8041 taacgatgac acgccaagtg ttttgataat gatataaatg aaagtaaagc aaattaaatg
     8101 aggaaatgta tagagcagtg catatagata aatctatgat tggattaaca ctaatataaa
     8161 tataaatgaa aaaccatcca cttaaacttt tgataagtta ggtggatggt tatttattgt
     8221 gttgttctaa aaatttggac atttatatag aaggaactat atctaatgtg aagataatgg
     8281 ctattcgctt caatgaaggc gacgtgtaaa agtgtatatt aaacgtttcg gggtcagact
     8341 ccgagaacag tgtccgtcat gcttaccgca tactaatgaa gcattattat atcagtgttt
     8401 atagccgatt taagataata aattttgcta aacaaaaaag ccaacccatg aatgttggat
     8461 tggcttttta catgcatctg aatctctaat tttaaaaaaa tatgaatata aataagacag
     8521 taaaaattaa atttcagttg ttgcaatttc ttcatctgta ggtacatcat cgttaaggcc
     8581 aacaagtgct tcagaaacat ttcgtgaatg ataaccgata cgttcaagaa caccaatcat
     8641 atcgatatat agtaatccgc cttttgttgt acattcacca cgattaaggc gtttaatatg
     8701 acctttgcgt agtttatgtt caatattaaa tgattctcta ctacgttcta caatttcatc
     8761 ttttttcgtt ttgtcataaa catctaacat gtcgatggct ttatcaaatg actcagcaac
     8821 atggttgaat aatttatcca taccgcgttg tgcatcttct gtaatgcgaa tatcttcatc
     8881 atgttggcgt tttaattgag cgacatactc ttctgttagc tctgctactt ttaaaataga
     8941 gcgattgaca tcaaacataa ctgctaaacg ctcaacgtca gccttcgtta tggcttttgt
     9001 agaaattcta actaaataat ttcgaatgct atcattgatt gtttcaacag cttggtgctt
     9061 ttgttcaagc tttttgatca attttttatc gtcttttgta atttcgcgaa tgtcttcaaa
     9121 cattgataag acaatctgac caacattttg taattctttt tgagtttctt gtaatgcaac
     9181 accaggtgcg tgataaacaa gatctttgtt taagtgctga ggtttatagt catcagcaat
     9241 atctttacct gggacaagct ttgtaacaat ccatgctaaa cctgctacaa atggtaattg
     9301 aatcaaagta tttgttatgt tgaagatacc atgtgatact gcaatcgtca tcgctggttt
     9361 taagtgccat acatcttgta acaaactaat caaatgaatc acaactggca agaaaattgt
     9421 gaagataatt accccgatta agttaaagat gacgtgtaca agcgccgcac gttttgcagc
     9481 gattgagccg gctaaactag ctaagatagc tgtaatcgtt gtaccaatat tatcgcctag
     9541 taacacaggg attgctgcgt ttaagctaat taaatcttgt tgataaaatt cttgtaaaat
     9601 accaatcgtc gcacttgaac tttgaactag tgctgttaac cctgcgccga caataacagc
     9661 aagtattgga tttgatgaca tatcaagcat taattgctta aatccatcta atgatgctaa
     9721 aggtttaacg gcatcaccca taaattctag accgaagaat agagaaccga aaccgaatag
     9781 tatgcggcca atgttattaa ttttagagcg tttaaagaaa aagattaaaa atgcacctaa
     9841 tgctaaaatt ggcattgcat attcgcctaa atctataccg ataataaatg cagttaccgt
     9901 tgttccgata ttagcaccca ttatcactcc aatggcttgt ttcaatgtca taaatccagc
     9961 tgttaccagt ccgattgtga taactgtcgt acctgaacta ctttgtatta aaatagttac
    10021 aacgatacct gcaataacac ctaatactgg atttgatgta aatttgttta aaatatctcg
    10081 tagcctgtct cctgctgatg cttgaagccc gtctcccatg atttttaagc cgtaaaggaa
    10141 aatacctaaa ccacctaaaa aggagaaaat gacttctgta accgacattt ccattatttc
    10201 acctcaaata agctttatat ttagattatc gcttataatt gtaaatttaa tgttaagatt
    10261 aggtaaaatt atttaacaat atatgttatt tgtatatgac ttgtaaaata tcgtcactta
    10321 ttatgtaaat tttcagtgtg aaatggcagg tttgcaatca cttgtttaac aaaatgatgc
    10381 aatcaatcat gtaattatgt ttcatcaaaa aaatcatgtg agtgggataa cgaaataaag
    10441 tttgtgatca tatcacttct atcccactcc atgatttgaa atcaccatat agacagattt
    10501 aatgtgcctc gttataacaa tttgtgttct tttaataatg actcaatgta cgtacctttt
    10561 atctttttaa ggaatcctgc taatgcgagt ttttgcattt tcgaatcttt agtaatctcg
    10621 cgcaaatctt gatggtcatt cagttcgtat atggcatcca ttaagacgcg aagatcaaat
    10681 gtactattga tgacctctgg aataccacga tctatattta gtaattgata aacagcttcc
    10741 atggcagtac gaaccgaata ttctgttgta aatacagtat ctcgctctgt ttctgcaaag
    10801 ttaccaataa atgctaaatt ttgtgattga tgcgggacga ctaaaggtct gtcgccgata
    10861 gcacgcgtca tgaaataaga ggtaatatat ggcatataaa caggaatcgt attagatgca
    10921 tgttttgcta agtcttcaat tttgtcagtt ggtacaccta agtgatacag ccattcttga
    10981 catatttcat taccactaca ttctgtgatt ggctttttaa tataatcgcc gtttacatct
    11041 gaatataagg cataaatcca tgtagatatt tcatttttag gttggtcttt aaactgttgc
    11101 tgacgattga ttgtaaaact gatttgccat gcagaatcat tgattgtaat aattccgcca
    11161 gtaaccgttt tgcctgcaag agggtcacgt ttacaaatgc tttctattgt atcgataata
    11221 tctttattgt ttgttgtcga tgttgcagaa acaaaccaac tcttttgagg aatattttgg
    11281 caaaacttat caggattacc aaattcagga ctttgtcgcg ctaaattttt ccatagtgtc
    11341 caactaccac ctaattcgtc agttggtggt gctggtgtat cattatcacc atacgtagag
    11401 ctttccgtaa tactaccgtt tgtcacaaag acaagatcat ctacagtcag tttaattgac
    11461 tctgctttgc catgtcggtg aattaatatt tctcgggcaa ttttttgact tgtcgtaaca
    11521 tctactttaa tatcttctac ttttacaccg tattcaaatt gaaccccatg cgattttaaa
    11581 tattcaacca taggtaatac taaagattca tattgattat atttagtgaa ttttaaagct
    11641 gaaaagtctg cgagaccacc aatatgatga acgaatcgca ttagatagcg acgcatttcc
    11701 atagcagaat gccacggttc aaatgcaaac atcgttttcc agtaaatcca aaagtttgaa
    11761 ttaaagaagt catcggaaaa tacatctgtt attttgacat catctaaatc ttcttcattc
    11821 gttaagcata aatctaaaat ttctttaatc gcttttttgg tcaaagtgaa gtctccatcg
    11881 gtaactaaac gttgaccctg tttttcaata actcgacagc gagaatagtt agggtcttct
    11941 ttgtttagcc aatagaactc atctaataca gacgcgttat cgatttctaa tgaagggata
    12001 gatctgaata agtcccacaa acattcaaag tggttctcca tttcacgacc acctcggaca
    12061 acatagcctt ttaaaggcat attttcacca tcaagactac cacctgcttt aggtaactct
    12121 tctaaaatat gaatcttcga accttccatt tgaccatccc ttattaaaaa acaagctgca
    12181 gcaagtgaag ctagaccaga tccgattaag taagcggatt tgttttctac attttcaggt
    12241 tttttagggc gcgcaaatgc ttcataattt ccataactgt aatacatatc caccaagtcc
    12301 tttcgtatta gaatacactc agactatacc cctttgtgaa ttgcaaatat tagtatttat
    12361 attggtaaat ttgacgaaat tagatcattt ttatattttt gatttttaaa attttatcac
    12421 tcgaatgttt ttggattctg aggtgcggga cttggaataa ttcgaatcat tatgtggcat
    12481 gttaaacagg taaatataag ctatgcacat ttaacaagcg gatactcctg acgcaattat
    12541 agtatcatta catattgttt aatcgtttgg ggtaatgtaa ttaaaaggta aaataggcag
    12601 agtgttatac ataaaatgta aagaaagagg cgatagaatg tctaaaatca ggtcttttac
    12661 aatattaagt ctacttattt acttagctat gatgtgctat acagtagtga cctattcaaa
    12721 attaccaacc aaagtaccta ttcattataa tttagcaggg gatgctgata actttgctga
    12781 taaatgggtg ctgcttttga ttaatagcgc atttatagtg atttggctta tatttttcat
    12841 tgcaggtaga tactatgaac gatttgccaa atggtcacat tataatcata caccacgtga
    12901 aattcgagcg attaaattat ttttaagtac gttaaattta gagattatga gctatatgtc
    12961 tatcttcaca gtattagaaa tttggcaaat acaacaccat catcaattca atttactatg
    13021 gtttaatatg atatttatca ttatcattgg tttgacgctt gtcatatttt gtttacttcc
    13081 tacaattcat aaaatgagag attctcaata aaaatataaa ctgtcatgat gcagttttga
    13141 agcggattcc tgagattatt ataaataata tctagtattt ataattaaaa tgatatatta
    13201 tttattatag ttaaatattg tgtatatttt gtgaactttt tgtaaaaatt cgattgcctg
    13261 tcacatatag gagtgttaca ttttaaatat gtgatcatcg caaaatataa gttgaaatag
    13321 gttgtagatt aatcaggatg ataaatattt tatataaaga gagggagtca ttatgacact
    13381 acttactgta aatccattcg ataatgttgg attatcagcc ttagttgcag cagtacctat
    13441 tattttattt ttattatgct taaccgtttt taaaatgaaa ggcatttatg cagcattgac
    13501 aactttggtt gttacattga ttgtggcttt atttgtattt gaattaccag tgcgtgtatc
    13561 agcaggtgcg attacagaag gcgttgttgc cggtattttc ccaataggat atatcgtttt
    13621 aatggcagtt tggttatata aagtttctat taaaacagga caattttcta ttattcaaga
    13681 tagtattgca agtatttcag aggaccaaag aatccaacta ttattaattg gattttgttt
    13741 caacgcattt ttagaaggtg cagcaggatt tggtgtgcca attgcgattt gtgcagtatt
    13801 attaattcaa cttggatttg aaccattaaa agcagcgatg ttatgcttaa ttgctaatgg
    13861 tgcggcgggt gcctttggtg caattggttt accagttagt attattgata cgtttaactt
    13921 aagtggaggc gttacaacat tagatgttgc gagatattca gcattaacac ttccaatttt
    13981 aaactttatt atcccatttg ttttagtatt cgttatagat ggcatgaaag gtattaaaga
    14041 aattttacct gtcattttaa cagtgagtgg tacatatact ggattacaat tattattaac
    14101 aatattccat ggtccagaac tagcagacat tatcccatca ctagcaacaa tggtggtgtt
    14161 agcatttgtt tgtcgtaaat ttaaaccgaa aaacattttc aggttgaaag aatcagaaca
    14221 taaaattcaa aaacgaacgc ctaaagaaat tgtctttgct tggagtccgt tcgtcatttt
    14281 aactgccttt gttttagtat ggagtgcacc attcttcaaa aaattattcc aacctggagg
    14341 tgcacttgaa agtttagtaa taaaattgcc aattccaaat actgtgagtg atttatcgcc
    14401 taaaggaatt gcgttgcgtc tcgatttaat tggtgcaact gggacagcga ttttattaac
    14461 agtaattatt acaattttaa ttacgaagct aaaatggaaa agtgcaggtg ctttattggt
    14521 cgaagcaatt aaagaattat ggttaccgat ccttacaatt tcagctatcc tagctattgc
    14581 taaagttatg acatacggtg gtttgactgt agcaattgga caaggtattg ctaaagctgg
    14641 agcaattttc ccattattct ctccagtatt aggttggatt ggtgtgttta tgactggttc
    14701 agttgtaaat aacaatactt tattcgcacc tattcaagcg acagtagcac aacaaatttc
    14761 aacaagcggt tcattacttg tggcagctaa cactgcaggt ggtgtagcag cgaaacttat
    14821 ttcaccacaa tcaattgcca ttgcgactgc agctgttaaa aaagttggtg aagaatctgc
    14881 attattaaaa atgacgctaa aatacagtat tatatttgtt gcttttattt gtgtttggac
    14941 gtttatacta acgttaatat tctaaatata aataatgttg tcacttggat tcaaatgaca
    15001 ttttaaatct aattattcat gaatcgaact agtacgaaat gcattgagca tcttgtctag
    15061 ttcgattttt taatgtctaa aaatgtcgta tatgtaatta gagtagaaag tgttgaggcg
    15121 tttcagaagt tgtttagaaa agtaagtaaa ataaaaaatg cactgagcaa caaaagatgt
    15181 tgctagtgca tttagatgat tcttatcatt tcaaataaga atgtgttaat caacgtatat
    15241 aagttaaaat tggtttggat aaaatgatat ctatcgttgt gtattgtttg tttttatagt
    15301 tcgcgacgac gtccagctaa taacgctgca cctaacgcta atgataatcc accaaataca
    15361 gttgtaccaa tgaatggatt ttcttcacca gtttctggta atgcttgagc tttgttagca
    15421 tctgcatggt ttgctggttg cttcttatca acaacaagtt cttgaccagg tttgatcatg
    15481 tttttatcag ctaatttgtt atctgcagca attttgtcag cagtagtgcc gtttgctttt
    15541 gcaatgtcat ttactgtatc accaggttta acgacatgta ctccgttgcc gtcttcttta
    15601 ccaggtttgt tgttgtcttc tttgccaggc ttgttgccat cttctttacc aggttttttg
    15661 ttgtcttctt taccaggttt gttgccgtct tctttgccag gcttgttgtt gtcttctttg
    15721 ccaggtttgt tgccgtcttc tttaccaggt ttgttgttgt cttcctcttt tggtgcttga
    15781 gcatcgttta gctttttagc ttctgctaaa atttctttgc tcactgaagg atcgtcttta
    15841 aggctttgga tgaagccgtt acgttgttct tcagttaagt taggtaaatg taaaatttca
    15901 tagaaagcat tttgttgttc tttgttgaat ttgttgtcag cttttggtgc ttgtgcatca
    15961 tttagctttt tagcttctgc taaaaggtta gcgctttggc ttgggtcatc ttttaagctt
    16021 tggatgaaac cattgcgttg ttcttcgttt aagttaggta aatgtaagat ttcatagaaa
    16081 gcattttgtt gttctttgtt gaatttgtta tccgctttcg gtgcttgaga ttcatttaac
    16141 tttttagctt ctgacaatag gttagcactt tgacttgggt catcttttaa gctttggatg
    16201 aaaccattgc gttgttcttc gttcaagtta ggcatgttca agatttcata gaaagcattt
    16261 tgttgttctt tgttgaaatt gttgtcagct ttcggtgctt gagattcgtt taatttttta
    16321 gcttcaccta aaacgttagt gctttggctt ggatcgtctt taagactttg aatgaaacca
    16381 ttgcgttgcg cttcgtttaa gttaggcatg ttcaagattt catagaaggc gctttgttga
    16441 tctttgttga agttattttg ttgcgcatca gcttttggag cttgagagtc attaagtttt
    16501 tgagcttcac ctaaaacgtt agcactttgg cttggatcat ctttaaggct ttggataaaa
    16561 ccattacgtt gatcagcgtt taagttaggc atatttaaca cttgataaaa agcattttgt
    16621 tgagcttcat cgtgttgcgc agcatttgca gcaggtgtta cgccaccaga tataagtaat
    16681 gtacctaaag ttacagatgc aatacctaca cctagtttac gaattgaata aatgtttttc
    16741 tttttcaaat taataccccc tgtatgtatt tgtaaagtca tcataatata acgaattatg
    16801 tattgca
//
LOCUS       DAMZKC010000046        16337 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.046, whole genome shotgun sequence.
ACCESSION   DAMZKC010000046 DAMZKC010000000
VERSION     DAMZKC010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 16337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16337
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.046"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(61..1413)
                     /gene="glmU"
                     /locus_tag="POB55_001123"
     CDS             complement(61..1413)
                     /gene="glmU"
                     /locus_tag="POB55_001123"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HDI6500096.1"
                     /translation="MRRHAIILAAGKGTRMKSKKYKVLHEVAGKPMVEHVLESVKGSG
                     VDQVVTIVGHGAESVKGHLGERSLYSFQEEQLGTAHAVQMAKSHLEDKEGTTIVVCGD
                     TPLITKETLETLIAHHEDANAQATVLSASIQQPYGYGRIVRNASGRLERIVEEKDATQ
                     AEKDINEISSGIFAFNNKTLFEKLTQVKNDNAQGEYYLPDVLSLILNDGGIVEVYRTN
                     DVEEIMGVNDRVMLSQAEKAMQRRTNHYHMLNGVTIIDPDSTYIGPDVTIGSDTVIEP
                     GVRINGRTEIGEDVVIGQYSEINNSTIENGACIQQSVVNDASVGANTKVGPFAQLRPG
                     AQLGADVKVGNFVEIKKADLKDGAKVSHLSYIGDAVIGERTNIGCGTITVNYDGENKF
                     KTIVGKDSFVGCNVNLVAPVTIGDDVLVAAGSTITDDVPNDSLAVARARQTTKEGYRK
                     "
     gene            complement(1436..1567)
                     /locus_tag="POB55_001124"
     CDS             complement(1436..1567)
                     /locus_tag="POB55_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500097.1"
                     /translation="MCLNDARIFEVRKKLFLKLQRIKNNAFYMLKEYCRLNYNNDEV"
     gene            complement(1757..2059)
                     /gene="spoVG"
                     /locus_tag="POB55_001125"
     CDS             complement(1757..2059)
                     /gene="spoVG"
                     /locus_tag="POB55_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HDI6500098.1"
                     /translation="MKVTDVRLRKIQTDGRMKALVSITLDEAFVIHDLRVIEGNSGLF
                     VAMPSKRTPDGEFRDIAHPINSDMRQEIQDAVMKVYDETDEVVPDKNATSEDSEEA"
     gene            complement(2131..2511)
                     /locus_tag="POB55_001126"
     CDS             complement(2131..2511)
                     /locus_tag="POB55_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDI6500099.1"
                     /translation="MKIINTTRLPEALGPYSHATVVNGMVYTSGQIPLNVDGEIVSAD
                     VQAQTKQVLENLKVVLEEAGSDLNSVAKATIFIKDMNDFQKINEVYGQYFNEHKPARS
                     CVEVARLPKDVKVEIELVSKIKEL"
     gene            complement(2528..3352)
                     /gene="purR"
                     /locus_tag="POB55_001127"
     CDS             complement(2528..3352)
                     /gene="purR"
                     /locus_tag="POB55_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HDI6500100.1"
                     /translation="MRYKRSERIVFMTQYLMNHPNKLIPLTFFVKKFKQAKSSISEDV
                     QIIKNTFQKEKLGTVITTAGASGGVTYKPMMSKEEATEVVNEVITLLEEKERLLPGGY
                     LFLSDLVGNPSLLNKVGKLIASIYMEEKLDAVVTIATKGISLANAVANILNLPVVVIR
                     KDNKVTEGSTVSINYVSGSSRKIETMVLSKRTLAENSNVLVVDDFMRAGGSINGVMNL
                     MNEFKAHVKGVSVLVESKEVKQRLIEDYTSLVKLSDVDEYNQEFNVEPGNSLSKFS"
     gene            complement(3366..4214)
                     /locus_tag="POB55_001128"
     CDS             complement(3366..4214)
                     /locus_tag="POB55_001128"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HDI6500101.1"
                     /translation="MIYETAPAKINFTLDTLFKRNDGYHEIEMIMTTVDLNDRLTFHK
                     RKDRKIVVEIEHNYVPSNHKNLAYRAAQLFIEQYQLKQGVTISIDKEIPVSAGLAGGS
                     ADAAATLRGLNRLFDIGASLEELALLGSKIGTDIPFCIYNKTALCTGRGEKIEFLNKP
                     PSAWVILAKPNLGISSPDIFKLINLDKRYDVHTKMCYEALENRDYQQLCQSLSNRLEP
                     ISVSKHPQIDKLKNNMLKSGADGALMSGSGPTVYGLARKESQAKNIYNAVNGCCNEVY
                     LVRLLG"
     gene            complement(4524..4787)
                     /locus_tag="POB55_001129"
     CDS             complement(4524..4787)
                     /locus_tag="POB55_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HDI6500102.1"
                     /translation="MPKSILDIKNSIDCHVGNRIVLKANGGRKKTIKRSGILKETYPS
                     VFIVELDQDKHNFERVSYTYTDVLTENVQVSFEEDNHHESIAH"
     gene            complement(4887..5780)
                     /gene="rsmA"
                     /locus_tag="POB55_001130"
     CDS             complement(4887..5780)
                     /gene="rsmA"
                     /locus_tag="POB55_001130"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HDI6500103.1"
                     /translation="MLDNKDIATPSRTRALLDKYGFNFKKSLGQNFLIDVNIINNIID
                     ASDIDAQTGVIEIGPGMGSLTEQLARHAKRVLAFEIDQRLIPVLNDTLSPYDNVTVIN
                     EDILKANIKEAVENHLQDCEKIMVVANLPYYITTPILLNLMQQDIPIDGYVVMMQKEV
                     GERLNAEVGSKAYGSLSIVVQYYTETSKVLTVPKSVFMPPPNVDSIVVKLMQRTEPLV
                     TVDNEEAFFKLAKAAFAQRRKTINNNYQNYFKDGKQHKEVILQWLEQAGIDPRRRGET
                     LSIQDFAKLYEEKKKFPQLEN"
     gene            complement(5791..6327)
                     /gene="rnmV"
                     /locus_tag="POB55_001131"
     CDS             complement(5791..6327)
                     /gene="rnmV"
                     /locus_tag="POB55_001131"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HDI6500104.1"
                     /translation="MKINEFIVVEGRDDTERVKRAVECDTIETNGSAINEQTLEVIRN
                     AQQSRGVIVLTDPDFPGDKIRSTITEHVKGVKHAYIDREKAKNKKGKIGVEHADLIDI
                     KEALMHVSSPFDEAYESIDKSVLIELGLIVGKDARRRREILSRKLRIGHSNGKQLLKK
                     LNAFGYTEADVRQALEDE"
     gene            complement(6494..7267)
                     /locus_tag="POB55_001132"
     CDS             complement(6494..7267)
                     /locus_tag="POB55_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDI6500105.1"
                     /translation="MLIDTHVHLNDEQYDDDLSEVITRAREAGVDRMFVVGFNKSTIE
                     RAMKLIDEYDFLYGIIGWHPVDAIDFTEEHLEWIESLAQHPKVIGIGEMGLDYHWDKS
                     PADVQKEVFRKQIALAKRLKLPIIIHNREATQDCIDILLEEHAEEVGGIMHSFSGSPE
                     IADIVTNKLNFYISLGGPVTFKNAKQPKEVAKHVSMERLLVETDAPYLSPHPYRGKRN
                     EPARVTLVAEQIAELKGLSYEEVCEQTTKNAEKLFNLNS"
     gene            complement(7298..9271)
                     /gene="metG"
                     /locus_tag="POB55_001133"
     CDS             complement(7298..9271)
                     /gene="metG"
                     /locus_tag="POB55_001133"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HDI6500106.1"
                     /translation="MAKETFYITTPIYYPSGNLHIGHAYSTVAGDVIARYKRMQGYDV
                     RYLTGTDEHGQKIQEKAQKAGKTEIEYLDEMIAGIKQLWAKLEISNDDFIRTTEERHK
                     HVVEQVFERLLKQGDIYLGEYEGWYSVPDETYYTESQLVDPQYENGKIIGGKSPDSGH
                     EVELVKEESYFFNISKYTDRLLEFYDQNPDFIQPPSRKNEMINNFIKPGLADLAVSRT
                     SFNWGVHVPSNPKHVVYVWIDALVNYISALGYLSDDESLFNKYWPADIHLMAKEIVRF
                     HSIIWPILLMALDLPLPKKVFAHGWILMKDGKMSKSKGNVVDPNILIDRYGLDATRYY
                     LMRELPFGSDGVFTPEAFVERTNFDLANDLGNLVNRTISMVNKYFDGELPAYQGPLHE
                     LDEEMEAMALETVKSYTESMESLQFSVALSTVWKFISRTNKYIDETTPWVLAKDDSQK
                     DMLGNVMAHLVENIRYAAVLLRPFLTHAPKEIFEQLNINNPQFMEFSSLEQYGVLTES
                     IMVTGQPKPIFPRLDSEAEIAYIKESMQPPATEEEKEEIPSKPQIDIKDFDKVEIKAA
                     TIINAEHVKKSDKLLKIQVDLDSEQRQIVSGIAKFYTPDDIIGKKVAVVTNLKPAKLM
                     GQKSEGMILSAEKDGVLTLVSLPSAIPNGAVIK"
     gene            complement(9556..10395)
                     /gene="rsmI"
                     /locus_tag="POB55_001134"
     CDS             complement(9556..10395)
                     /gene="rsmI"
                     /locus_tag="POB55_001134"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HDI6500107.1"
                     /translation="MAVLYLVGTPIGNLADITYRAVDVLKRVDMIACEDTRVTSKLCN
                     HYDIPTPLKSYHEHNKDKQTAFIIEQLELGLDVALVSDAGLPLISDPGYELVVAAREA
                     NIKVETVPGPNAGLTALMASGLPSYVYTFLGFLPRKEKEKSAVLEQRMHENSTLIIYE
                     SPHRVTDTLKTIAKIDATRQVSLGRELTKKFEQIVTDDVTQLQALIQQGDVPLKGEFV
                     ILIEGAKANNEISWFDDLSINEHVDHYIQTSQMKPKQAIKKVAEERQLKTNEVYNIYH
                     QIS"
     gene            complement(10397..10645)
                     /locus_tag="POB55_001135"
     CDS             complement(10397..10645)
                     /locus_tag="POB55_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDI6500108.1"
                     /translation="MDSHFVYIVKCSDGSLYTGYAKDVNARVEKHNRGQGAKYTKVRR
                     PVHLVYQEMYETKSEALKREYEIKTYTRQKKLRLIKER"
     gene            complement(10638..11363)
                     /locus_tag="POB55_001136"
     CDS             complement(10638..11363)
                     /locus_tag="POB55_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HDI6500109.1"
                     /translation="MLKENERFDQLIKEDFSIIQNDDVFSFSTDALLLGHFTKPRTKD
                     IVLDLCSGNGVIPLLLFAKHPRHIEGVEIQKTLVDMARRTFQFNDVDEYITMHHMDLK
                     NVTKVFKPSQYTLVTCNPPYFKENQQHQHQKEAHKIARHEIMCTLEDCMIAARHLLKE
                     GGRLNMVHRAERLMDVLFEMRKVNIEPKKVVFIYSKVGKSAQTIVVEGRKGGNQGLEI
                     MPPFYIYNEDGNYSEEMKEVYYG"
     gene            complement(11637..11984)
                     /gene="yabA"
                     /locus_tag="POB55_001137"
     CDS             complement(11637..11984)
                     /gene="yabA"
                     /locus_tag="POB55_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HDI6500110.1"
                     /translation="MDRNEIFEKIMRLEMNVNQLSKETSELKALAVELVEENVALQLE
                     NDNLKKVLGNDEPTTIDTANSKPAKAVKKPLPSKDNLAILYGEGFHICKGELFGKHRH
                     GEDCLFCLEVLSD"
     gene            complement(12001..12804)
                     /locus_tag="POB55_001138"
     CDS             complement(12001..12804)
                     /locus_tag="POB55_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HDI6500111.1"
                     /translation="MPNVIGVQFQKAGKLEYYTPNDIQVDIDDWVVVESKRGIEIGIV
                     KNPLMDIAEEDVVLPLKNIIRVADDKDIDKFNCNERDAENALILCKDIVREQGLDMRL
                     VNCEYTLDKSKVIFNFTADDRIDFRKLVKILAQHLKTRIELRQIGVRDEAKLLGGIGP
                     CGRSLCCSTFLGDFEPVSIKMAKDQNLSLNPTKISGACGRLMCCLKYENDYYEEVRAQ
                     LPDIGEAIETPDGNGKVVALNILDISMQVKLEGHEQPLEYKLEEIETMH"
     gene            complement(12805..13731)
                     /locus_tag="POB55_001139"
     CDS             complement(12805..13731)
                     /locus_tag="POB55_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HDI6500112.1"
                     /translation="MDEQQQLTNAYHSNKLSHAYLFEGDDAQTMKQVAINFAKLILCQ
                     TDSQCETKVSTYNHPDFMYISTTENAIKKEQVEQLVRHMNQLPIESTNKVYIIEDFEK
                     LTVQGENSILKFLEEPPDNTIAILLSTKPEQILDTIHSRCQHVYFKPIDKEKFINRLV
                     EQDMSKPVAEMISTYTTQIDNAMALNEEFDLLALRKSVIRWCELLLTNKPMALIGIID
                     LLKQAKNKKLQSLTIAAVNGFFEDIIHTKVNVEDKQIYSDLKNDIDQYAQKLSFNQLI
                     LMFDQLTEAHKKLNQNVNPTLVFEQIVIKGVS"
     gene            complement(13945..14274)
                     /locus_tag="POB55_001140"
     CDS             complement(13945..14274)
                     /locus_tag="POB55_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HDI6500113.1"
                     /translation="MKMIIAIVQDQDSQELADQLVKNNFRATKLATTGGFLRAGNTTF
                     LCGVNDDRVDEILSVINQTCGNREQLVSPITPMGGSADSYIPYPVEVEVGGATVFVMP
                     VDAFHQF"
     gene            complement(14302..14919)
                     /locus_tag="POB55_001141"
     CDS             complement(14302..14919)
                     /locus_tag="POB55_001141"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HDI6500114.1"
                     /translation="MSAFITFEGPEGSGKTTVINKVYHRLVKDYDVIMTREPGGVPTG
                     EEIRKIVLEGNDMDIRTEAMLFAASRREHLVLKVIPALKEGKVVLCDRYIDSSLAYQG
                     YARGIGVEEVRALNEFAINGLYPDLTIYLNVSAEVGRERIIKNSRDQNRLDQEDLKFH
                     EKVIEGYQEIIHNESQRFKSVNADQPLENVVEDTYQTIIKYLEKI"
     gene            complement(14921..16258)
                     /locus_tag="POB55_001142"
     CDS             complement(14921..16258)
                     /locus_tag="POB55_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HDI6500115.1"
                     /translation="MKQPILNKLESLNQEEAISLHVPGHKNMTIGHLSQLSMTMDKTE
                     IPGLDDLHHPEEVILESMKQVEKHSDYDAYFLVNGTTSGILSVIQSFSQKKGDILMAR
                     NVHKSVLHALDISQQEGHFIETHQSPLTNHYNKVNLSRLNNDGHKLAVLTYPNYYGET
                     FNVEEVIKSLHQLNIPVLIDEAHGAHFGLQGFPDSTLNYQADYVVQSFHKTLPALTMG
                     SVLYIHKNAPYRETIIEYLSYFQTSSPSYLIMASLESAAEFYKTYDSTVFFDKRAQLI
                     ECLEKKGFEMLQVDDPLKLLIKYEGFTGHDIQNWFMNAHIYLELADDYQVLAVLPLWH
                     HDDTYLFDSLLRKIEDMILPKKSVSKVKQTQLLTTEGNYKPKRFEYVTWCDLKKAKGK
                     VLARHIVPYPPGIPIIFKGETITENMIELVNEYLETGMIVEGIKNNKILVEDE"
BASE COUNT         4946 a         3293 c         2168 g         5930 t
ORIGIN      
        1 attacattaa tattacatag gattatcctt ttatcctagt cattttaaat acgtaaatga
       61 ttatttccta tatccttctt ttgttgtttg tcttgctctt gccacagcta aactgtcatt
      121 tggtacgtca tctgtgattg tggaaccagc tgccaccaat acatcatcac caattgttac
      181 aggtgctact aaattaacat tgcaacctac aaatgaatct ttgccgacga tagttttaaa
      241 tttattttca ccatcatagt taactgtaat cgttccgcaa ccaatattag tacgttcacc
      301 aattacagca tcgccaatat aacttaaatg tgaaaccttg gcaccatctt taagatctgc
      361 ttttttaatt tctacaaaat ttccaacctt aacatctgca cctaattgcg cgcctggtct
      421 caattgcgca aacggtccga ccttagtatt cgctcctacg ctagcatcat taacaacaga
      481 ctgttgaata catgcaccat tttcaatcgt actattgtta atttcagagt actgaccaat
      541 aacaacatct tcgccaattt ctgtacgacc attaattcgt acgcctggtt caatgactgt
      601 atcactacca attgtaacgt ctggaccaat ataagtgctg tcaggatcga tgattgtcac
      661 accatttagc atgtgataat gattcgtacg acgttgcatc gccttctcag cctgactaag
      721 cattacacga tcatttacac ccatgatttc ttcaacatca ttggtacgat agacttctac
      781 gatgccgcca tcatttaaaa ttaacgacaa tacatcaggg aggtaatatt caccttgcgc
      841 attatcattt ttcacttgtg ttaatttttc aaacaacgtt ttattattaa acgcaaaaat
      901 acctgaacta atttcattaa tatccttttc agcttgcgtt gcatctttct cttcaactat
      961 gcgttctaaa cgacctgacg catttcgaac gattcttccg tatccatatg gttgttgaat
     1021 cgatgcagat aatacagttg cttgagcatt agcatcctca tgatgcgcaa tcaatgtttc
     1081 taatgtttcc tttgtgatga gcggtgtgtc accacataca acgatcgtcg taccttcctt
     1141 gtcttctaag tgtgatttcg ccatttgcac tgcatgcgca gtaccgagtt gttcctcttg
     1201 aaaactgtat aaagaacgct cgcctaaatg tccttttaca ctttcagcac catgtcctac
     1261 gatggttaca acttgatcga caccagagcc tttcacactt tccaatacat gttcgaccat
     1321 aggtttccca gcaacctcgt gtagcacttt atacttttta gatttcattc ttgtgccttt
     1381 acctgctgcc aaaattatcg cgtgtcttcg catgaacatt aacccccatt aaattttaca
     1441 cttcgtcatt attataattt aatctgcaat actctttcaa catataaaag gcattgtttt
     1501 taattcgttg taattttaaa aacaactttt ttcttacttc aaaaatacga gcatcattca
     1561 aacacaattt ttatcagcaa tgaatcaata attttcatat catcataatc tgttaacgta
     1621 tatatttatt agcctttcat taaaaataaa aaaacattaa tacaaactaa aaaaactgcc
     1681 gacaaagctt tatgctttat cgacagtatc aaatcaactt attgcaaatg tattacatcg
     1741 ctaaatataa aattgattaa gcttcttctg aatcttctga tgtagcgttt ttatctggta
     1801 ctacttcatc tgtttcatca tatactttca tcactgcatc ttgaatttct tgtctcatat
     1861 ctgaattaat aggatgcgcg atgtcgcgga attcaccatc tggtgtacgt ttacttggca
     1921 ttgcaacgaa caagccagag tttccttcaa ttacacgtaa atcatgaatt acgaaagctt
     1981 catctaatgt aatggaaacg agtgctttca ttcgtccatc tgtttgtatt tttctaagtc
     2041 ttacatctgt cactttcatg tagtgagccc ccctatagta tatatctctc tgttttattt
     2101 agaagcttga ttaaacatat taatcgaaaa ttataattcc ttaattttac ttactaattc
     2161 aatttctact ttcacatctt ttggcaaacg cgcaacctct acacaactac gcgctggctt
     2221 gtgttcatta aaatattgac catacacttc atttattttt tggaaatcat tcatatcttt
     2281 aatgaaaatg gtcgctttcg caacagaatt caaatcagat cctgcttctt ccaaaacaac
     2341 ctttaaattt tctaaaactt gttttgtctg tgcttgaaca tcagcgctta cgatttcccc
     2401 atcaacattc aatggaatct gaccagaagt ataaaccata ccattcacaa ctgttgcatg
     2461 cgaatatggt ccaagtgctt ccggtaatct tgttgtgtta atgattttca taatactaaa
     2521 actcctttta tgaaaactta gataaactgt tgccaggttc tacgttaaac tcttgattat
     2581 attcatctac atcagataat ttcactaagg aagtataatc ttcaatcaat ctttgtttaa
     2641 cttcttttga ttctacaagt actgataccc cttttacatg ggctttaaac tcattcatta
     2701 aattcataac accattaata gagccaccag ccctcataaa atcatcgaca actaaaacat
     2761 ttgaattttc tgctaaagtt ctcttcgaaa gtaccattgt ctctattttt cttgaagatc
     2821 ctgaaacgta attaattgaa actgtagaac cttcagtcac cttgttgtct tttctaatca
     2881 ctactactgg taaatttaaa atattagcaa ccgcatttgc caatgaaata ccttttgtcg
     2941 caatggtaac aacagcatct aatttttctt ccatgtaaat actggcaatt aacttaccaa
     3001 ctttgtttag tagcgatgga ttacctacca aatctgataa aaataaatac ccgccaggta
     3061 acaaacgttc tttctcttct aatagagtaa tgacctcatt aacaacttca gtcgcctctt
     3121 ctttactcat cattggttta tacgtaacac caccacttgc gccagcagta gtaattactg
     3181 tacctaactt ttctttttgg aatgtatttt ttataatttg gacatcttca cttattgaag
     3241 acttcgcctg tttaaatttt ttcacaaaaa aagttaatgg aatcaattta ttcggatggt
     3301 tcatcaaata ttgcgtcata aaaacaattc tctcgcttcg tttatatctc atcttttcaa
     3361 cccttctatc ctaatagtct aactaagtac acttcattac aacaaccgtt aactgcatta
     3421 taaatatttt ttgcttggct ttcttttcgt gctagcccat acacagtcgg tccgcttcca
     3481 ctcattaacg caccatctgc accacttttc aacatattat tttttaattt atcgatttgt
     3541 gggtgttttg aaacagaaat tggctctaat cgattagaca aactttgaca taattgttga
     3601 taatctcgat tttctaaggc ctcataacac attttcgtat gtacgtcgta acgcttatct
     3661 aaattgatca acttaaatat atctggtgat gatatgccta agtttggttt agcaagaatc
     3721 acccaagctg aaggtggttt atttaaaaac tcgattttct ctcctcttcc agtacatagt
     3781 gcagttttat tataaataca aaacggaata tctgtcccga ttttactgcc tagtagagcc
     3841 aattcttcca aactcgcccc tatatcaaaa agtcgattca atcctcttaa cgttgctgct
     3901 gcatcagccg aacctccagc taagccagca gaaacaggta tttctttatc gatagaaatt
     3961 gttacacctt gctttagttg atattgctca ataaatagtt gcgctgcacg atatgcgaga
     4021 tttttatgat tagaaggcac ataattatgt tcaatctcaa caactatctt tcgatctttt
     4081 cttttatgaa aagttaaacg atcatttaaa tcaactgttg tcattatcat ttcaatctca
     4141 tgatagccat catttctttt aaaaagtgta tcgagcgtaa aattaatttt ggctggtgcc
     4201 gtttcatata tcattttccg ccctcttttc gttatcttaa tatgatgatt ttaacataaa
     4261 gagaattaat atttgacatt ttaatttgtt ttttagtgtt tttttaataa aaaagtaatt
     4321 ttctccatct aattacttag tatttacttg tatttgtgtt tataaataca agtatccatt
     4381 tactgaaaaa tctataagcg aaacatcagt gttaaacatt ttgtaaataa aaagagtatt
     4441 gaacataacg cccaacactc aaataattat cataatattt acttcttata ctaagaaacg
     4501 tctaacatct ctatatgtct tatttagtgt gcaattgatt cgtgatgatt atcctcttca
     4561 aatgaaactt gaacattttc agttaacaca tcagtgtatg tataagatac tctctcaaag
     4621 ttgtgtttgt cttgatctaa ctcaacaatg aaaactgacg gatatgtttc ttttaaaatt
     4681 ccagaacgtt ttattgtttt cttacggcct ccattggctt tcagtacaat acgatttcct
     4741 acatgacaat caatagaatt tttgatgtcc aaaattgatt ttggcatatt gctccacctc
     4801 gctacaagtt acataatacc atatttgcag cgttttcgtc aaaaaaaatt taaattttaa
     4861 caatagtgct ttgctttgtc aatcatttaa ttttctaatt gagggaattt tttcttttct
     4921 tcatacaatt tagcaaaatc ttgaatagat agcgtttcac cgcgacgtct tggatcaata
     4981 cctgcttgtt ccaaccattg taaaatcact tctttgtgtt gtttaccatc tttaaaataa
     5041 ttttgatagt tattgttaat tgtctttctt ctttgtgcaa atgctgcttt tgctaactta
     5101 aagaatgctt cctcgttatc tactgttact aacggttcag ttctctgcat cagttttaca
     5161 actattgaat caacattagg tggtggcata aatacagatt taggtaccgt taatacttta
     5221 ctagtctctg tatagtattg tacgacaatt gataacgaac catatgcttt tgaacctact
     5281 tcagcattta agcgttcgcc cacttctttt tgcatcatca ccacgtagcc atcaattggt
     5341 atatcttgtt gcattaaatt taataaaatt ggcgtcgtaa tatagtacgg caggtttgca
     5401 acaaccatta ttttttcgca atcttgtaaa tgattttcaa cagcttcttt aatattcgct
     5461 tttaaaatat cttcattaat caccgtcaca ttatcataag gtgatagtgt atcatttaat
     5521 acaggtatta aacgttgatc aatttcaaat gccaatactc ttttagcatg tctagccaat
     5581 tgttctgtca atgaccccat gcctggacca atttcaatca ccccagtttg tgcatcaata
     5641 tcacttgcat caatgatatt attaatgata ttcacatcta tcaaaaagtt ctgtcctaaa
     5701 ctttttttaa aattaaagcc atatttatct aacaacgctc gcgttcttga tggtgttgca
     5761 atatctttat tatccaacat tttcacttcc tcattcatct tctaaagctt gccttacatc
     5821 cgcttcggta taaccaaatg catttaactt tttcaataac tgcttaccat tggaatggcc
     5881 gattcgcaat tttctactta aaatttctct acggcgcctt gcatctttcc caacgattaa
     5941 ccccaactct attagcacag atttatcaat tgattcataa gcttcatcaa agggtgaact
     6001 aacatgcatt aacgcttctt taatatcaat taagtcggca tgttcaacac caattttccc
     6061 ttttttattt ttagcttttt ctctatcaat atacgcatgt ttaacacctt tgacatgttc
     6121 agtaattgta cttctaattt tatctcctgg gaaatctgga tctgttaata caataacgcc
     6181 tcgactttgt tgagcatttc taattacttc taaagtttgt tcgttgatgg cactaccatt
     6241 cgtttcaatc gtatcacatt caacagctcg tttaacacgc tcagtatcat ctcgtccttc
     6301 tacaactata aactcattga ttttcattta aaacgccttc ctacaattct taatttcaaa
     6361 gctctaacca tctctatcgc acgtattcca catgcacgat attttaattt aaaaaagtga
     6421 gaaagaacca taggatatta aacgaatata gcatcgttta catttatggc ggtgatcttt
     6481 ctcactttta actttatgaa tttaaattaa acaatttctc tgcattttta gttgtttgtt
     6541 cgcacacttc ttcataagat aagcctttta attcagcaat ttgttcagct actaaagtta
     6601 ctctcgccgg ttcatttcgc ttccctctat acggatgtgg cgaaagatac ggtgcatcgg
     6661 tttcaactag caaacgctcc attgacacat gcttagcaac ttctttaggc tgtttagcat
     6721 ttttaaatgt cacaggtcct cctaatgaaa tataaaaatt cagcttatta gttacaatat
     6781 ctgcaatttc tggagaacca ctaaagctat gcataatccc gcctacctct tcagcatgct
     6841 cctccaataa gatatcgata cagtcttgag ttgcttcacg gttatgaatg ataattggta
     6901 acttcaaacg cttagctaaa gcaatttgct ttctaaaaac ttccttttga acatctgcag
     6961 gagatttatc ccagtgataa tctaatccca tttcaccaat accaatcact tttggatgct
     7021 gagccaaaga ttcaatccat tccaggtgtt cttctgtaaa atcaattgca tcaactggat
     7081 gccaaccgat aatgccatat aaaaaatcat actcatcgat taatttcatc gcgcgttcaa
     7141 ttgtcgattt gttaaaacca actacaaaca tacgatcaac acctgcttct ctagcacgtg
     7201 taatcacttc actcaaatca tcatcgtatt gctcatcatt taaatggaca tgtgtatcga
     7261 ttaacataat tatctctcct aatttttaaa aatacagtta tttaatcact gcaccatttg
     7321 gaattgcact tggtaaactt actaaggtta atacaccatc tttttcagca gataatatca
     7381 taccttcaga tttttgtccc attaatttag ctggtttcag gttagtaaca actgctactt
     7441 ttttaccaat aatatcatct ggtgtataga atttggcaat tcctgataca atttgtcttt
     7501 gttcagaatc taagtctact tgaattttta aaagcttatc tgacttctta acatgttcag
     7561 cattaataat cgttgctgcc ttaatttcaa ctttatcaaa gtctttaata tcaatttgag
     7621 gtttgctagg aatctcttct ttttcctctt cagtagcagg cggttgcatt gattctttga
     7681 tatatgcaat ttccgcttcg ctatccaatc ttgggaaaat aggtttaggt tgcccagtaa
     7741 ccataattga ctcagtaagc acaccatatt gctctaaact actaaattcc ataaattgag
     7801 gattgttaat gttcaattgt tcaaaaatct ctttcggcgc atgtgttaag aatggacgta
     7861 ataatacagc tgcataacga atattttcaa ctaagtgagc cattacattg cctaacatat
     7921 ctttttggct atcgtcctta gctaataccc aaggcgttgt ttcgtcaata tacttattcg
     7981 ttctacttat aaacttccat accgtagata atgccacaga aaattgcaaa ctttccatgc
     8041 tttcagtgta gcttttcact gtttctaaag ccatagcttc catttcttca tctaattcat
     8101 gaagtggacc ttgatacgct ggtaattcgc catcaaagta cttattaacc atagaaatcg
     8161 tacggtttac taagttacct aagtcatttg ctagatcgaa atttgtacgc tcaacaaatg
     8221 cttcaggtgt aaatacgcca tctgaaccaa atggtaattc acgcattaga taataacgtg
     8281 tagcatctaa accatagcga tcaattaaaa tattagggtc tacgacatta cctttagatt
     8341 tactcatttt tccatctttc atcaaaatcc aaccatgtgc aaagactttt ttaggtaacg
     8401 gtaagtctaa tgccatcaat aaaataggcc aaataattga gtggaatcgc acaatttcct
     8461 tagccattaa atgaatatct gctggccagt atttgttaaa tagtgactca tcatctgata
     8521 aatagcctaa tgctgaaata tagttaacta acgcatcaat ccaaacataa acaacatgtt
     8581 ttggattaga cggaacatgg acaccccaat taaatgatgt acgagaaaca gccaaatcag
     8641 caagtcctgg tttaatgaag ttgttaatca tttcattttt tcttgatggt ggttgtataa
     8701 aatctgggtt ttggtcatag aattctaata gacggtctgt atatttacta atattaaaga
     8761 aataactttc ttctttaact agttcaactt cgtgtccaga atctggactt ttgccaccaa
     8821 taattttacc gttttcgtat tgtgggtcta ctaattgtga ctctgtatag tatgtttcat
     8881 ccggaacaga ataccaacct tcatattcac ctaaatagat atcaccttgc tttaataaac
     8941 gttcaaacac ttgctcaacg acatgtttat gacgttcttc agttgttctg ataaaatcat
     9001 catttgaaat ttcaagctta gcccacaatt gtttaattcc agcaatcatc tcatccaaat
     9061 attcaatttc tgtcttacca gctttttgag ctttttcttg aattttttga ccgtgttcat
     9121 ccgttccagt caaataacga acatcatatc cttgcattct cttatatctt gcaataacat
     9181 ctccagccac tgtagaatat gcatgtccta tatgtaaatt cccactagga tagtatattg
     9241 gggttgttat ataaaatgtt tctttagcca taactgttcc tcctcgtaat gtctcacttc
     9301 aaataatatc taagatttct atgcatttca acggacgcga acatttatct cgtcatctca
     9361 ttttcatctt attcaatgtt aaggtattta ttactttcaa tgtatttatt ttacattaaa
     9421 aaacgtcgat gtcatcattt ttttatcata gtcaacatca ttttatcagt atcaatacaa
     9481 aatgataact taccatttaa aattacagct tgcgtttata aaatcgttta aaatttcata
     9541 taatcgataa agtgattaac ttatttgatg ataaatatta tatacttcat tcgttttaag
     9601 ttgtcgttct tcagcaactt ttttaatagc ttgttttggt ttcatctgtg aagtttgaat
     9661 ataatgatca acatgctcat tgatagataa atcatcaaac cacgatatct cattgttcgc
     9721 tttagcaccc tcaatcaaga taacgaattc gcctttcaat ggtacatcgc cttgctgaat
     9781 caatgcttgt aattgtgtta catcatcagt tacaatttgt tcgaacttct tagtcaattc
     9841 acgccctagt gatacttgtc gtgttgcatc tatctttgca attgttttta atgtatctgt
     9901 cacacgatgc ggtgattcgt atataattaa tgtgctattt tcatgcatac gttgctctaa
     9961 tacagcactt ttttctttct cttttcgtgg caaaaatcct aaaaatgtat atacatatga
    10021 aggtaatcca ctagccatca aagccgtcag cccagcatta ggtccaggca cagtctctac
    10081 tttaatatta gcttctctgg ctgccactac taattcgtat ccaggatcac taattaaggg
    10141 caatccagca tcagatacga gcgcaacgtc aagacctaat tctaactgtt caatgataaa
    10201 agcagtctgc ttatccttgt tatgttcgtg atatgacttt aatggagttg gaatatcata
    10261 atgattacac agcttactag ttactctagt gtcttcacaa gcaatcatat caacacgttt
    10321 caatacatca actgctctat aagtaatatc tgctaaatta ccaattggtg tgcccactaa
    10381 atataataca gccatactat cgctccttaa ttaatcgcaa tttcttttgt ctggtataag
    10441 ttttaatttc atattcacgc ttcaatgctt cagactttgt ctcatacatt tcttgataaa
    10501 ctaaatgcac cggacgtctt actttcgtat atttggctcc ttgacctcgg ttatgttttt
    10561 caacacgtgc attaacgtct ttagcgtatc ctgtatataa acttccatca ctacatttta
    10621 caatatatac aaaatgacta tccataatat acttccttca tttcttcgct ataattacca
    10681 tcttcattat aaatataaaa tgggggcatg atttctaaac cttgatttcc accttttcga
    10741 ccttctacta ctatcgtttg tgctgatttc cctactttac tatatataaa aacgactttc
    10801 ttaggttcaa tattcacttt tctcatttca aacaagacat ccattagtct ctctgcacga
    10861 tgtaccatgt ttagcctgcc accttctttt aataaatgac gggctgcaat catgcaatct
    10921 tcaagtgtac acataatctc atgtctcgct atcttatgtg cttctttttg atgttggtgt
    10981 tgctgattct ctttaaaata aggtggatta cacgttacta aagtatattg tgaaggttta
    11041 aatactttag taacgttttt caaatccatg tgatgcattg ttatatattc atcaacatca
    11101 ttgaattgaa atgtgcgtcg cgccatatcg acaagtgttt tttgaatctc aacaccttct
    11161 atatgtcgtg gatgtttcgc aaacaataac aacggtatca ccccattgcc tgaacataag
    11221 tccaacacaa tatcttttgt tctaggtttt gtaaaatgcc ctaacaacaa agcatccgtt
    11281 gaaaacgaaa aaacatcatc attttgaata atactaaaat cttctttgat tagttgatca
    11341 aatcgttcat tctcttttaa catttttaac tcctatgaag acaacctttt tctattgagt
    11401 aattgaaatc tctttttata ttatatgact aactacattc gatgtatttt tgacttttaa
    11461 cagctacatt ttataactaa tgatactgcg tgcacactta ataataacat ttagtaccgc
    11521 catctcttat gcaatcacca aaactttata cacaattaaa gcctgaaaca tgcattgtct
    11581 cagacttatc caaaccatat tcatttataa ttataacact atttgagtgt gcttgattaa
    11641 tcacttaaaa cttctaaaca gaacagacaa tcttcaccat gtcgatgttt tccaaataat
    11701 tcgcctttac aaatatgaaa tccttctcca tacaatatag ccaaattatc tttacttggt
    11761 aatggctttt tcacagcttt tgctggtttt gaattcgcag tatcaatagt agttggttca
    11821 tcattgccca acaccttttt caaattatca ttttcaagtt gaagcgctac attttcttct
    11881 actaattcaa ctgcaagtgc ctttaattct gatgtttcct ttgaaagttg attgacattc
    11941 atttctaaac gcattatttt ttcaaatatt tcattgcgat ccaaatgtaa taatgcctcc
    12001 ttaatgcata gtttctattt cttctaattt atattcaagt ggctgttcat gtccctcaag
    12061 cttcacctgc atagaaatgt ctaatatatt taaagcaact actttcccgt taccatcagg
    12121 cgtttcaatt gcttcaccaa tatcaggtaa ttgtgcacgt acttcctcat aatagtcatt
    12181 ttcatatttt aaacaacaca tcaaacgacc acatgcacca gaaattttag ttggatttaa
    12241 tgataaattt tgatccttag ccatcttaat cgatactggt tcaaaatctc ctaaaaatgt
    12301 agaacaacat aacgacctac cacaaggtcc gataccgcca agcaatttgg cttcatccct
    12361 tacaccaatt tgtctcaact cgatacgtgt ttttaaatgt tgcgctaata tttttactaa
    12421 ttttctaaaa tcaatacgat catccgccgt aaaattaaaa ataactttcg atttatctaa
    12481 tgtatattcg caattgacta aacgcatgtc caaaccttgt tctcttacaa tgtctttaca
    12541 tagtattaat gcattttcag catctcgttc attacaatta aatttatcaa tatctttgtc
    12601 atcagcaacg cgaataatat ttttaagagg taacacaaca tcctcttcag caatatccat
    12661 taatggattt ttaacaatac ctatctctat gcctctttta gattcgacaa ctacccagtc
    12721 atctatatct acttgtatat cattaggtgt ataatattct aattttcccg ctttttgaaa
    12781 ctgaacacct attacatttg gcatctaact cacaccctta attacgattt gttcaaatac
    12841 aagcgttgga tttacatttt gattcaattt cttatgtgct tccgtcagtt gatcaaacat
    12901 caaaattaat tgattaaacg acaacttttg cgcatattga tcaatatcat tttttaaatc
    12961 actatatatt tgtttatcct ctacatttac ctttgtatgt atgatatctt cgaagaaacc
    13021 attcacagct gcaatagtta aagattgcag ttttttattt ttagcctgtt tcaataaatc
    13081 aataatacct ataagtgcca ttggcttatt agtaagcaac aattcacacc aacgtataac
    13141 tgatttcctt aatgctaata aatcaaattc ttcatttaaa gccattgcat tatctatttg
    13201 cgtagtataa gtactaatca tttcagctac tggcttagac atgtcttgtt caactaatct
    13261 atttataaac ttttctttat caataggctt gaaatataca tgctgacacc ttgaatggat
    13321 tgtgtctaaa atttgctcag gttttgtaga caataaaata gcaatcgtat tgtccggtgg
    13381 ttcttcaaga aatttcaaga tactgttttc cccttgaaca gttaactttt caaagtcttc
    13441 aatgatgtac actttatttg tgctttctat aggaagttga ttcatatgac gcacaagttg
    13501 ttcaacttgt tctttcttaa ttgcattctc agttgttgat atatacataa agtctggatg
    13561 attatatgta ctaacctttg tttcacattg actatctgtt tgacataaaa taagctttgc
    13621 aaaattaatc gcaacttgtt tcatcgtttg tgcatcatca ccttcaaata aataggcatg
    13681 cgataattta tttgaatgat atgcattcgt caattgttgc tgttcatcca ttcattacaa
    13741 actccctttt gcttttatat aaaaaggttg ccaaagagca actaattact gcaacaatag
    13801 cgaccatgat ttgtgattat ttttagaacc acaaaaatca aatctattgg catctgaact
    13861 agtcgcagtc aagcaaccca aaattgttaa taacttatta atacactatt ttaagtatct
    13921 ctaattgatg attgtattat agaattaaaa ttgatggaat gcatcaactg gcataacaaa
    13981 tacagtagca ccgccaactt caacttcaac tggatatgga atgtacgaat ccgcactgcc
    14041 tcccataggt gtaataggtg aaaccaactg ttctctatta ccacacgttt gattaatcac
    14101 agacaatatt tcatctacac ggtcatcatt gacaccacat aagaatgttg tattacctgc
    14161 tcttaaaaac ccacctgttg ttgccaattt tgttgctcta aagttatttt taacaagttg
    14221 atctgcaagt tcctgactat cttgatcttg tacgatcgct ataatcattt tcattttata
    14281 acacctcttc taacaattat atcatatctt ttctaaatat ttgatgatag tttgatacgt
    14341 gtcttcaaca acattttcaa gaggttgatc tgcattaacg cttttgaacc gttgtgattc
    14401 attatgaatg atttcttggt aaccttcaat tactttttcg tgaaacttta aatcttcttg
    14461 atctaatcta ttttgatctc ttgaattttt aataatacgt tcgcggccta cttcagcact
    14521 aacattcaaa taaatcgtca agtctggata taatccattt attgcaaatt cgtttaatgc
    14581 tcttacttct tcaacgccaa tccctctagc ataaccttga taagctaatg aactatcgat
    14641 atagcgatca cacaacacaa ccttaccttc ttttaaagct ggtatgacct ttaatacaag
    14701 atgttctctt ctagatgcag caaataacat tgcttcagtt ctaatgtcca tatcattgcc
    14761 ttctaataca attttacgta tttcttcacc agtaggaacg ccgcccggtt ctcttgtcat
    14821 aatgacatca taatctttta ctaatctatg gtaaacttta ttaattacag ttgtttttcc
    14881 agagccttct gggccctcaa aagttataaa agctgacatt ttattcatcc tcaactaaaa
    14941 ttttattatt tttaattcct tcaactatca ttccagtttc cagatattca tttactaatt
    15001 ctatcatatt ttcagttatt gtttctcctt tgaaaataat aggaatccct ggcggatacg
    15061 ggacaatatg tcgcgccaga actttacctt ttgccttttt caaatcacac caagtaacat
    15121 attcaaagcg ttttggttta tagttacctt cagttgttaa aagttgtgtt tgtttaactt
    15181 tagaaactga ttttttcggt aaaatcatat cttcaatttt acgtaaaagc gaatcaaata
    15241 aatacgtatc atcatgatgc cataacggca ataccgctaa tacttgatag tcgtccgcta
    15301 attctaaata gatatgtgca ttcataaacc aattttgaat atcatgacct gtaaaacctt
    15361 catattttat cagcaacttc aacggatcat caacttgaag catttcaaaa cccttcttct
    15421 ccaaacattc gattaattgc gctctcttat caaaaaacac ggtactatca tatgttttat
    15481 agaactcggc agctgactct aaactagcca taatcaaata cgaaggacta gatgtttgga
    15541 agtagcttag atattctata atagtttctc tataaggtgc atttttatga atataaagta
    15601 ccgagcccat cgttaaagct ggtaacgttt tatgaaaaga ttgaacaaca tagtcagctt
    15661 gataatttaa tgtagaatct ggaaatcctt gcaatccaaa gtgcgcgccg tgtgcttcgt
    15721 caatgagtac aggaatattt aattggtgca aagatttgat aacctcttct acattaaatg
    15781 tttcaccgta atagttagga taagtcaaca cagcaagttt gtgaccgtca ttattcaaac
    15841 ggcttaaatt aactttatta taatgattcg ttaacggact ttgatgcgtt tcaataaaat
    15901 gcccttcttg ttggctaata tcgagcgcat gtaatacaga tttatgtaca tttcttgcca
    15961 ttaagatatc gccttttttc tgtgaaaaag actggatgac agataatatt cctgaagtgg
    16021 tgccattcac taagaaataa gcatcataat ctgaatgttt ctccacctgc ttcatacttt
    16081 ccaaaatgac ttcttcagga tgatgtaaat catctaatcc aggtatttca gttttatcca
    16141 ttgtcattga taattgagat aaatgaccga tagtcatatt tttatgaccc ggaacatgca
    16201 aagaaatcgc ttcttcttga tttaaacttt ctaatttatt taaaataggt tgcttcatga
    16261 tatacgcttc ctttatttac actgttttgg aattagttac tttcaaaagt attaattata
    16321 tagtaacact tctttga
//
LOCUS       DAMZKC010000047        15914 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.047, whole genome shotgun sequence.
ACCESSION   DAMZKC010000047 DAMZKC010000000
VERSION     DAMZKC010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 15914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15914
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.047"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            69..540
                     /gene="sigS"
                     /locus_tag="POB55_001143"
                     /pseudo
     CDS             69..540
                     /gene="sigS"
                     /locus_tag="POB55_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500398.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigS"
     gene            653..1096
                     /locus_tag="POB55_001144"
     CDS             653..1096
                     /locus_tag="POB55_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComK"
                     /protein_id="HDI6500116.1"
                     /translation="MQDNSTKYLLYIQTATSNHLETNCVFLHCDYILKVPINKLVSYY
                     AKLHLSSQSVLIETAKNILNINKLVPIYINPKTILFPLKHKRAPIQIYINAHYIVGMT
                     AIENSTLIHFQEGIQLEVDEPFSLVSKKCHESLALKHFIENTISN"
     gene            complement(1083..1526)
                     /locus_tag="POB55_001145"
     CDS             complement(1083..1526)
                     /locus_tag="POB55_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500117.1"
                     /translation="MNKASFDKKVKKQLWFLNKKEKQALDQRLSSISDDDSVNLNKPV
                     TFANAYLRQNVFRNKETKSYSMFVTLVVMMFAYVALLGLFLFGLITSLSGVQFFVSPK
                     VDLSTTVVILTIIGAILLMIVSIYFIKIVTSYFTKKLLEIKFNSK"
     gene            1822..2457
                     /gene="sdpA"
                     /locus_tag="POB55_001146"
     CDS             1822..2457
                     /gene="sdpA"
                     /locus_tag="POB55_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease SdpA"
                     /protein_id="HDI6500118.1"
                     /translation="MQKFKDFFYDDLSVTRGNYFLTLMAAFFITIILFIGIVVSEVHL
                     LYSMLIVLVGLILLRLFKINLFSFKKLTLSQVIYIIGGALLIYGLDNLYLYFHDVPAN
                     EQQLEQEIRNTPFYISIFTVTIIPAIVEEIVFRGMIIRVIFRKHLFLGLIVSSLVFAS
                     LHESDTWIGYLPYLYSGLIFGIIYIKTKRLEVVIFMHFLNNLLALLFIIWG"
     gene            complement(2897..3610)
                     /locus_tag="POB55_001147"
     CDS             complement(2897..3610)
                     /locus_tag="POB55_001147"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HDI6500119.1"
                     /translation="MAKLNVEVFADGADIEEMKAAYKNKQVDGFTTNPSLMAKAGVTD
                     YKAFAEEAVKEIPDASISFEVFADDLETMEKEAAILKQYGENVFVKIPIVNTKGESTI
                     PLIKKLSADNVRLNVTAVYTIEQVKEITEAVTEGVPTYVSVFAGRIADTGVDPLPLMK
                     EAVKVTHSKDGVKLLWASCRELFNVIQADEIGADIITCPADVVKKVNTNLGRDINELS
                     VDTVKGFAKDIQSSGLSIL"
     gene            3870..4172
                     /locus_tag="POB55_001148"
     CDS             3870..4172
                     /locus_tag="POB55_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500120.1"
                     /translation="MSRSKKYFYLSSLMIILSFFFNTNNVFLSGLFNSFIKLILFCSV
                     INSIILILSIIFADRSIKSLKPDADWIRIASKSLPWIILIVILVHIFSIVRTFGFI"
     gene            4428..4793
                     /gene="crcB"
                     /locus_tag="POB55_001149"
     CDS             4428..4793
                     /gene="crcB"
                     /locus_tag="POB55_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HDI6500121.1"
                     /translation="MQYVYIFIGGALGALLRYLISFLNTDGGFPIGTLIANLTGAFVM
                     GLLTALTIAFFSNHPTLKKAITTGFLGALTTFSTFQLELIHMFDHQQFITLLLYAVTS
                     YVFGILLCYVGIKLGGGLS"
     gene            4790..5143
                     /locus_tag="POB55_001150"
     CDS             4790..5143
                     /locus_tag="POB55_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HDI6500122.1"
                     /translation="MISIILVMIGGGFGAITRSAITDYFNHKFTSKLPIATLIVNLVG
                     SFLIGLNIGLSISISWFPAFFVTGFLGGLTTFSTLAKELTLMMTPKFNINLFLNYSLL
                     QFIIGFIACYIGYHI"
     gene            complement(5526..6359)
                     /locus_tag="POB55_001151"
     CDS             complement(5526..6359)
                     /locus_tag="POB55_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDI6500123.1"
                     /translation="MEVKTFYNGNTMPQIGLGTFRVENDENCMESVKYAIEQGYRSID
                     TAKVYGNEEQVGAGIRAGLESTGIAREDLFITSKLYFEDFGRENVAAAYEASLSRLGL
                     KYLDLYLVHWPGTNEAVMVDTWKGMEDLYKNNKVKNIGVSNFEPEHLEALLAQVSIKP
                     VINQVEYHPYLTQHKLKLYLAAQHIVMESWSPLMNAQILNDETIKDIAQELGKSPAQV
                     VLRWNVQHGVVTIPKSVTPNRISENFQIFDFELSDEQMTRIDGLNQDKRIGPDPKTFE
                     G"
     gene            complement(6571..7479)
                     /locus_tag="POB55_001152"
     CDS             complement(6571..7479)
                     /locus_tag="POB55_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="HDI6500124.1"
                     /translation="MDLSSPIVIGLIIAVVIALIFFVLFLVTLGSKKKVKRQTEEKYE
                     QQEQNIKKSHEEALEKERIQNKKTITKQQEDYNHMVSTKDREIDALKLFSKNHSEYVT
                     DMRLIGIRERLVKEKRIRPEDMHIMANIFLPKDGFNNIERISHLVLTRTGLYIIDSQL
                     LKGHVYNGISGGQFKDLPPMEQVFDTLDLDKSRPQTIVMDQNDDKRSLSFVNYSDQIE
                     AIKQLAEDLQKELGAKYTPTSILYFNPKNEGDVTISNYNQNSAVKVLVGAEQLDEFFN
                     KFVFHGRIQYNVEDLQQMMDKIESFN"
     gene            complement(7604..8797)
                     /locus_tag="POB55_001153"
     CDS             complement(7604..8797)
                     /locus_tag="POB55_001153"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HDI6500125.1"
                     /translation="MLNNKRLFTSESVTEGHPDKIADQVSDAILDAILKDDPNARVAC
                     ETTVTTGMALIAGEISTTTYVDIPKVVRETIKEIGYTRAKYGYDYETMAILTAIDEQS
                     PDIAQGVDKALEYRDKDSEEEIEATGAGDQGLMFGYATNETETYMPLAIYLSHQLAKR
                     LSDVRKDGTLNYLRPDGKVQVTVEYDENDNPVRIDTIVVSTQHAEDVTLEQIQEDIKA
                     HVIYPTVPENLINEQTKFYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGCF
                     SGKDPTKVDRSAAYAARYVAKNIVAAGLADQCEVQLAYAIGVAEPVSIAIDTFGTGKV
                     SEGQLVEAVRKHFDLRPAGIIKMLDLKQPIYKQTAAYGHFGRTDVLFPWEKLDKVEEL
                     KDAVK"
     regulatory      complement(8903..9014)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            9169..10761
                     /gene="pckA"
                     /locus_tag="POB55_001154"
     CDS             9169..10761
                     /gene="pckA"
                     /locus_tag="POB55_001154"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HDI6500126.1"
                     /translation="MSVDTYTETTKIDKLLKKPTSHFQLSTTQLYNKILDNNEGVLTE
                     LGAVNASTGKYTGRSPKDKFFVSEPSYRDNIDWGEINQPIDEETFLKLYHKVLDYLDK
                     KDELYVFKGYAGSDKDTMLKLTVINELAWHNLFAKNMFIRPESKEEATKIKPNFTIVS
                     APHFKADPEVDGTKSETFVIISFKHKVILIGGTEYAGEMKKGIFSVMNYLLPMQDIMS
                     MHCSANVGEKGDVALFFGLSGTGKTTLSADPHRKLIGDDEHGWNKNGVFNIEGGCYAK
                     AINLSKEKEPQIFDAIKYGAILENTVVAEDGSVDFEDNRYTENTRAAYPINHIDNIVV
                     PSKAAHPNTIIFLTADAFGVIPPISKLNKDQAMYHFLSGFTSKLAGTERGVTEPEPSF
                     STCFGAPFFPLHPTVYADLLGELIDLHDVDVYLVNTGWTGGKYGVGRRISLHYTRQMV
                     NQAISGKLKNAEYTKDSTFGLSIPVKIEDVPKTILNPINAWSDKEKYKAQAEDLIQRF
                     EKNFEKFGEKVEHIAEKGSFNK"
     gene            complement(11139..11885)
                     /locus_tag="POB55_001155"
     CDS             complement(11139..11885)
                     /locus_tag="POB55_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HDI6500127.1"
                     /translation="MPIESFTHQFEEITYLSDDLQVKALMMTPHHEVNRIVVYLRGGK
                     GQVGRVRAGRLMQFSDSQTLVIGPYYRGNNGSEGKDEFYRGDLNDVTQLLRLLHDKYP
                     HAFIHMVGFSRGGLQGLLTFQDLPVTSYTIWGGVSDIDLMYEERVDLRGMLRRMIGHP
                     KKDRAAYEARQAIPNINENSPPILIVHGGKDQQVGIHHAYYLADQLELKGATHETFYQ
                     MAEGHVPRPPAMVETLTYIKEFMNQVELHS"
     gene            complement(11890..12363)
                     /gene="ytkD"
                     /locus_tag="POB55_001156"
     CDS             complement(11890..12363)
                     /gene="ytkD"
                     /locus_tag="POB55_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase YtkD"
                     /protein_id="HDI6500128.1"
                     /translation="MKFRDKDNRQVNLTFKKDNEIADGNHVLAIPTFKNQLLFTKHNL
                     RGIEFPGGKRERGESSAEAVTRELYEETGAKVKNIHYIAQYTIETHDQTDFVKDVYFI
                     EVESLVSKNDYLETAGPVLFNCINDIELAQRSFLLQDSTILKCVERVQSLGFYQT"
     gene            12429..12686
                     /gene="yidD"
                     /locus_tag="POB55_001157"
     CDS             12429..12686
                     /gene="yidD"
                     /locus_tag="POB55_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HDI6500129.1"
                     /translation="MKKIFLAMIHFYQRFISPLTPPTCRFYPTCSEYTREAIQYHGAF
                     KGLYLGIRRILKCHPLHKGGFDPVPLKKDKSASKHSHKHNH"
     gene            complement(12683..13684)
                     /gene="menC"
                     /locus_tag="POB55_001158"
     CDS             complement(12683..13684)
                     /gene="menC"
                     /locus_tag="POB55_001158"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HDI6500130.1"
                     /translation="MKLTALHFYKYSEPFKSQIVTPKVTLTHRDCLFIELIDDKGNAY
                     FGECNAFQTDWYDHETIASVKHVIEQWFEDNRNKSFETYEAALKLVDSLENTPAARAT
                     IVMALYQMFHVLPSFSVAYGATVSGLSNKQLESLKATKPARIKLKWTPQIMHQIRVLR
                     ELEFHFQLVIDANESLDRQDFTQLQLLAREQVLYIEEPFKDISMLDEVVDGTIPPIAL
                     DEKATSLLDIINLIELYNVKVVVLKPFRLGGIDKVQTAIDTLKSHGVKVVIGGMYEYG
                     LSRYFTAMLARKGDYPGDVTPAGYYFEQDVVAHSGILKEGRLEFRPPLVDITQLQPY"
     gene            complement(13689..15167)
                     /gene="menE"
                     /locus_tag="POB55_001159"
     CDS             complement(13689..15167)
                     /gene="menE"
                     /locus_tag="POB55_001159"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HDI6500131.1"
                     /translation="MDFWLYKQAQQNGHHIAITDGQESYTYQNLYCEASLLAKRLKAY
                     QQSRVGLYIDNSIQSIILIHACWLANIEIAMINTRLTPNEMKNQMRSIDVQLIFCTLP
                     LELRGFQIVSLDDIEFAGTDITMNGLLDNTMDIQYDTSNETVVPKESPSNILNTSFNL
                     DDIASIMFTSGTTGPQKAVPQTFRNHYASAIGCKESLGFDRDTNWLSVLPIYHISGLS
                     VLLRAVIEGFTVRIVDKFNAEQILTIIKNERITHISLVPQTLNWLMQQGLHEPYNLQK
                     ILLGGAKLSATLIETALQYNLPIYNSFGMTETCSQFLTATPEMLHARPDTVGMPSANV
                     DVKIKNPNKEGHGELMIKGANVMNGYLYPTDLTGTFENGYFNTGDIAEIDHEGYVMIY
                     DRRKDLIISGGENIYPYQIETVAKQFPGISDAVCVGHPDDTWGQVPKLYFVSESDISK
                     AQLIAYLSQHLAKYKVPKHFEKVDTLPYTSTGKLQRNKLYRG"
     gene            complement(15326..15781)
                     /locus_tag="POB55_001160"
     CDS             complement(15326..15781)
                     /locus_tag="POB55_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4909 domain-containing protein"
                     /protein_id="HDI6500132.1"
                     /translation="MKICLMLYLLIFLSSCGTNNKIVGQRFEIQQHNETIGSIYFSAD
                     YAHIRGIEKGTVKYFVDKVGSKRYLFIEYIPDNVLNCKSDFWKTLKYKKDKITYYVYL
                     IENLDDEVFHLSVLQDMNRIPIDIADDVATMGKSPHQNDRMTLKLNKNN"
BASE COUNT         5083 a         2914 c         2286 g         5631 t
ORIGIN      
        1 tttctaaata ttttattcta actttatccg gaaatatttg ttatacgtat aacttggaga
       61 atgactactt gaaatttaat gacgtataca acaaacacca caaaatcata caccatcttt
      121 taaaaaaaat ataatattag ctataattat gatgagtatt atcaactact cttgataaaa
      181 atgtggcaat tgagtcagat atataaaccc tcaagcaagc aatctttatc ctctttttta
      241 ttcactcgat taaattttta ccttatcgat ttattcagac aacaaaatca attaaaagat
      301 gtcattttat gtgagaataa ttcaccaaca ttaactgaac aaccaacata ctttaatgaa
      361 catgaccttc gtttacaaga tatcttcaag cttttaaatc aaagagaaag actatggctc
      421 aaactatacc ttgaaggata caagcaattt gaaattgctg aaatcatgtc attatcgctt
      481 tcaacgatta aattaattaa gatgtccgtt aagcgtaaat gccaacataa ttttaattag
      541 tttattgtga aatttcggtc gatgtgttaa tgatgttgaa acaccctaac atcttcatat
      601 aaatttattt taaccattaa attaacaact tattatacga ggagtactat atttgcaaga
      661 caattctact aaatatctac tttatatcca aactgctact tcaaaccatc tcgaaacaaa
      721 ttgtgtcttt ttacattgcg attacattct taaagttcca attaacaaac tcgtttcata
      781 ttatgcgaaa ttgcatttat catcacaaag tgtgctaatt gagactgcaa aaaacatact
      841 aaatattaat aaactggttc ctatttacat caacccaaaa actatacttt ttccgttaaa
      901 acataaacgt gcaccgatac aaatttatat caatgcacat tatattgttg gtatgactgc
      961 catagagaat tcaacattaa tacattttca agaaggcatt cagctagaag ttgatgagcc
     1021 attttctcta gtttcaaaaa aatgtcatga aagtttagct ttgaagcact tcattgaaaa
     1081 tactatttcg aattaaattt aatttctaaa agtttcttag taaagtatga tgtcacaatt
     1141 tttataaaat atatgctaac aatcattaat aaaatcgctc caataattgt taaaataaca
     1201 acagttgttg ataaatctac cttaggacta acgaaaaatt gtacgccaga tagacttgta
     1261 attagcccaa ataaaaacaa gcctaataaa gcaacataag caaacatcat cactactaat
     1321 gtaacaaaca tactataact ttttgtttct ttatttcgaa atacattttg tcttaagtaa
     1381 gcattagcaa aggtaacagg cttatttaag ttaacgctgt catcatcaga tatagaagac
     1441 aatcgttgat ctaatgcttg tttctccttc ttgttcaaaa accataattg ctttttaact
     1501 tttttatcaa atgatgcttt attcataatt aaggatttct ctcctttata atattgacgt
     1561 aattttaaac attagcgtca attgaaagtg tattttatat attttacaga aatatatgta
     1621 tcaaatctac aaaaatccat attacaattt catttatggt gatttgacgc taaaatacag
     1681 tctgctaatt ttccaaacta atgcttttta ggtaggtaca atgataaatt ttaatcgtat
     1741 atatgtttca tatataatga atctatcctt aaacatattg cattcaaaat aattttaaaa
     1801 aatgaaaaga aaggatttag catgcaaaaa ttcaaagact ttttttacga tgatttatcg
     1861 gttacacgag gaaattattt tttaacttta atggcagcat tttttattac tatcatttta
     1921 tttatcggca tagttgtcag tgaagtacat ttactttata gcatgctaat tgtattagta
     1981 ggtttaattc tattgaggct attcaaaatc aatttattct cttttaaaaa attaacattg
     2041 tctcaagtta tttatattat aggcggtgca ctattaattt atgggttaga taatctttat
     2101 ttatattttc atgacgtacc ggcaaatgaa caacaattag agcaagaaat acgaaataca
     2161 ccattctata tttctatttt cactgttacc atcatccccg ctattgtgga agaaattgtt
     2221 tttcgcggta tgataataag ggttatcttc agaaaacact tgtttttagg gttaattgtg
     2281 tctagtttag tttttgcatc attacacgaa tctgacactt ggattggtta tttaccttac
     2341 ttatattctg gtttgatttt tggtataatt tatataaaaa caaaacgatt agaagtggta
     2401 atattcatgc acttcttaaa taacttgtta gctctgctct ttataatatg gggataattt
     2461 tgagataacc tttcatatta ttttcaaatt atctatctga tatttatttt attgaacttg
     2521 agtcaccact taaactcaaa aatacgtgtc atcaagtgca aaataattta gaactaatac
     2581 aaacataaaa acttttgctt tacgcttaaa atcaaaagtc acatacctag atataagtac
     2641 aacttgtata ttttgtactc taataggtat gtggcttaaa taatatttaa ttcttaagtt
     2701 gcatatagta attgttttta ttcattttct aaaatatgcg agactaattt ccttttctac
     2761 tcctgttgaa tcaatttaga cttttttata actcgagctt ttacttagct gaattttgac
     2821 ttcttcttca ttaataacta ttatggaaaa acccccataa gcacaatgcc tataagtgtt
     2881 taaatatatt tatatattat agaatagaaa gacctgaaga ttgaatatct ttcgcaaagc
     2941 ctttaactgt atctactgat aattcgttaa tatcgcgacc taagtttgta ttcacttttt
     3001 tcacaacatc tgcagggcat gtaataatat ctgcaccaat ttcatcagct tgaatcacat
     3061 tgaataattc gcggcaactt gcccataata atttaacgcc gtctttacta tgcgtaactt
     3121 ttacagcctc tttcattaat ggtaatggat ctacgcctgt atctgcaata cgtcctgcaa
     3181 atactgaaac atatgttggc acaccttcag ttactgcttc agttatttct ttaacttgtt
     3241 caattgtgta aacagccgta acgtttaatc tcacattgtc agctgaaagc tttttaatta
     3301 aaggaatcgt tgattcacct tttgtattta caataggaat tttaacaaat acattttcgc
     3361 catattgttt tagaattgct gcttcttttt ccatagtttc taaatcgtct gcaaatactt
     3421 caaatgaaat tgaagcatct ggaatttctt tcacagcttc ttcagcaaaa gctttgtaat
     3481 ctgttacgcc cgctttcgcc attaaactag gatttgttgt aaaaccatcc acttgtttgt
     3541 ttttataagc tgctttcatt tcttcaatat ctgcaccgtc cgcaaatact tctacattta
     3601 gtttagccat ataatattgc ctccttgatt cttattaaaa ttttaacaac atctgcatgt
     3661 ctttttctta caaccatttg taaaaaatga tttttatttc tttgttatat aaaacattta
     3721 tatcacgctt ttataatata tagctaattt tttgcctata tttttaaaaa atgactttag
     3781 taaacattgt gtctacaagt taataaaact attgttttat gattgtttat gatacgatat
     3841 gtaaatgata accatccagg aggtctagca tgtctcgttc aaaaaaatac ttttacttat
     3901 ctagcttaat gattatttta agctttttct ttaatacaaa taacgttttc ctaagtggac
     3961 tttttaattc ttttattaaa ttaatacttt tctgcagtgt tattaactca attatactaa
     4021 ttttgtctat aatttttgca gatcgttcaa ttaaatcact aaagcctgat gcagattgga
     4081 ttagaattgc gagtaaaagt ttgccttgga ttattctaat tgttatttta gtacatatct
     4141 tttcaattgt tcgtacattc ggttttattt aaaaaagata aaatgtcaat gtagccttaa
     4201 tacaaaacaa tacaatgtat catgctataa tgagtaaaac aatttgatga cgttgttgcg
     4261 tataaaaaat attagatttt cgaaatcata actatacatc taatcgctat agttatacaa
     4321 caagatataa catataatga ggtttgataa tgcatcgaca atttttgtcg tcgcgttgcc
     4381 aaaacctctt ttttaaattc aaactacttc ttttcgaggt gaaccaaatg caatatgtat
     4441 atatttttat cggtggtgct ttaggcgctt tattacgtta cctcatttct tttctgaata
     4501 ctgacggagg ttttccaatc ggaacactga tagccaattt gactggtgcc tttgtaatgg
     4561 gattgctaac agccttaaca attgcatttt tttcaaacca tccgacgcta aaaaaagcta
     4621 ttacgactgg ttttcttggt gctttaacga ctttttcaac atttcaatta gaattaatac
     4681 atatgtttga tcatcaacaa tttataactt tactactata tgctgtaaca agttatgtct
     4741 ttggtatttt gttatgttac gtcggtataa aactaggtgg tggtttatca tgatatcaat
     4801 cattttagtc atgattggcg gcggtttcgg tgcaattact agaagtgcca ttactgatta
     4861 ttttaatcat aaatttactt caaagttacc tatcgcaaca ttgatagtaa atctagttgg
     4921 tagtttttta attggattaa atataggctt atcaatttca atctcatggt tccctgcgtt
     4981 ctttgttacc ggttttttag gtggcttaac aactttctca acgttagcca aggaacttac
     5041 actaatgatg acgccaaaat ttaatattaa cctttttctc aattattcac ttttacaatt
     5101 catcattgga tttatagctt gttatattgg ctatcatatt taaaaataaa atgcttcatt
     5161 cagcaaatag gtaaattacg acaccttcct gaacgaagca ttttttaatt ttcatgcaaa
     5221 ttttttagca ccataaaatg cctaccaaat ttcaataatc tttgttaccg tttaaataat
     5281 gtgaatgtca ataaattctc caaactagtc gaaaataaag ggagtgggac agaaatgata
     5341 ttttctcaaa atttatttcg tcgtcccacc ccggcaaggt tgactagaat tgaaaaaagc
     5401 ttgttacaag cgcattttcg ttcagtcaac tactgccaat ataacttcgt agagcataga
     5461 acattgattt atgtcccagc ctgtgacaat ccatctaacc ttttcatcag ttgagtgatt
     5521 ttaatctatc cttcaaaagt ttttggatca ggtccaattc tcttatcttg atttaaacca
     5581 tcaattcgcg tcatttgttc atctgataat tcgaaatcaa atatttggaa gttttcagag
     5641 attctgtttg gtgttaccga tttagggatt gtaaccacac catgctgcac attccatctt
     5701 aaaacaactt gggcaggtga ctttcctaat tcttgagcaa tgtctttaat tgtctcatca
     5761 tttaaaattt gtgcattcat caatggtgac caagattcca tcacgatatg ttgtgctgcc
     5821 aaatataatt tcaatttatg ttgcgttaaa tatggatgat attcaacctg attaattaca
     5881 ggtttaattg acacttgtgc caacaaagct tccaaatgtt caggttcaaa attgctgaca
     5941 cctatatttt taactttatt atttttatat aaatcttcca tacctttcca tgtatcaacc
     6001 attacggctt cgttcgtacc tggccaatgt actagataca aatctaagta ttttaaacct
     6061 aatctagata aactagcttc gtaagcagct gctacatttt cacgaccgaa atcctcaaaa
     6121 tataattttg aagtaataaa taagtcttct ctagcaatac cagttgactc caatccggca
     6181 cgaatgccag cacctacttg ttcttcattc ccataaactt ttgcggtatc aatactacga
     6241 tatccttgtt caatggcata cttaacactt tccatgcaat tttcatcatt ttccacacga
     6301 aatgtcccta aaccaatttg tggcatcgtg tttccattat aaaatgtttt aacctccata
     6361 aatatcgcct cacctttttg atgtattata ccctgttatc ataacaaatc tgagttgaat
     6421 acatgagaaa aaacacttag agcaatcaac cactaaaatt ctagtaatat ctctcaaata
     6481 ttaatcaaat tgtaaaagta attctgttta atttatgaca aactaaaaaa gccgaagtaa
     6541 caacatatag tcatcacttc agcctaacat ttaattgaat gattcaattt tatccatcat
     6601 ttgttgtaag tcttccacgt tgtattgaat acgaccatgg aatacaaatt tgttaaagaa
     6661 ctcgtctaat tgttcagcac cgacaagcac tttgacagca ctattttgat tataatttga
     6721 aatcgttaca tcgccttcat ttttaggatt aaagtataaa attgaagttg gcgtatattt
     6781 ggcacctaat tctttttgta agtcttcagc caattgttta atcgcctcaa tttgatctga
     6841 ataatttaca aaagataatg aacgtttgtc atcattttga tccatcacaa tagtttgtgg
     6901 tctagattta tctaaatcca atgtatcaaa tacttgttcc attggtggta aatctttaaa
     6961 ttgaccgcca ctaataccat tataaacatg accttttaac aattgagaat caataatata
     7021 aagaccagtt cttgttaata ctaaatgact aattcgttca atattattaa agccatcctt
     7081 tggtaaaaag atatttgcca taatgtgcat atcttctggt cgaattcgtt tttctttaac
     7141 taatctttca cgaataccaa ttaatctcat gtccgttaca tattcactat gatttttcga
     7201 gaacaatttt aatgcgtcaa tctcacgatc ttttgtacta accatgtgat tataatcttc
     7261 ttgttgtttt gtaattgtct ttttattttg aatacgctct ttctctaaag cttcttcatg
     7321 agacttttta atgttttgtt cttgttgttc atacttttct tctgtttgtc gcttaacttt
     7381 tttcttacta cctaaggtaa ctaagaaaag gacaaaaaag attaatgcaa tgactactgc
     7441 aataatgagt ccaatgacta tcggtgaaga taaatccatc acaacaacgc tcctttttaa
     7501 tatatgaata actttaatta taatagaaaa gctaaagatt ttcgatacat attatcattt
     7561 atataccgaa aatcttttat ttagctatat tcaattcatc ttattatttt actgcgtctt
     7621 ttaattcttc cactttgtct aatttttccc atgggaataa gacatctgta cgtccaaaat
     7681 gaccataagc agcagtttgt ttgtaaatcg gttgtttcaa atcaagcatt ttaataatac
     7741 ctgcaggtct taggtcaaag tgttttctaa ctgcttcgac aagttgccct tcagaaactt
     7801 tacctgttcc aaatgtatca attgcaattg acactggttc tgcaacacca atcgcatatg
     7861 ccaattgtac ttcacattga tctgctaaac ctgctgcaac aatattttta gccacataac
     7921 gtgcagcgta tgcagctgaa cggtctactt ttgtaggatc cttaccactg aagcatccgc
     7981 caccatgacg tgcatagcca ccgtacgtat caacaatgat tttacgtcct gttaatcctg
     8041 catcaccttg aggtccaccg attacaaagc gtcctgtagg attgatgtag aatttagttt
     8101 gttcattaat caagttttct ggaacagttg gataaatgac atgtgcttta atgtcttctt
     8161 gaatttgttc aagtgtcaca tcctcagcat gttgtgttga tacgacaatc gtatcaatac
     8221 gtactgggtt atcattttca tcatattcaa cagtgacctg aactttaccg tctggtcgta
     8281 aataatttaa cgtaccatct ttacgcacat ctgataaacg ttttgccaat tgatgtgata
     8341 aataaattgc tagaggcata tacgtctctg tttcattcgt tgcgtaacca aacattaaac
     8401 cttggtcacc tgcacctgtt gcttcaattt cttcttcgct atctttatca cgatactcta
     8461 atgctttatc cacgccttgt gcaatgtcag gtgattgttc atcaatcgca gttaaaattg
     8521 ccattgtttc ataatcataa ccatattttg ctcttgtgta tccaatttct ttaattgttt
     8581 ctctaacaac tttcggaata tcaacatatg ttgttgtaga aatttcgccg gcgatcaatg
     8641 ccatacctgt tgtaacagtt gtttcacaag ctacacgtgc atttgggtcg tcttttaaaa
     8701 tagcatctaa tattgcatct gacacttggt cagcgatttt atctgggtgt ccttctgtaa
     8761 cagactctga agtaaataat cgtttgttat ttaacatagt ttgctccttt aaatttatat
     8821 tacgaaaatt ctctctctgt gagctaaata aaaaagacct tctaactatt aatatagaga
     8881 gaaggcctaa tacgtccatt cgctcttatc gttcagacct atttgtctgc aaacggtttg
     8941 gcacctttct tttataaaaa aagaggttgc tgggtttcat tgggtccatg tccctccacc
     9001 actcaggata agagaatccg ttaaaaataa tagtacctaa ttaatgaatt aatgtcaatt
     9061 tttcacaaat aaatttacag taaaatattg tagattaatt atgttaatgt gttatactaa
     9121 ttaaatgtaa aggcttacat ttaaattatc gctttggagg gatttaggat gtcagtagac
     9181 acatacactg aaacaactaa aattgacaaa ttactgaaaa aaccaacgtc acattttcaa
     9241 ctttcgacga cacaacttta taataaaatc ttagacaata acgaaggggt attaacagaa
     9301 cttggtgctg ttaatgcaag tactggaaaa tatactggtc gttcgcctaa agacaaattt
     9361 tttgtctctg aaccttcata tagagataac attgattggg gagaaattaa tcaacctatc
     9421 gatgaagaaa ctttcttgaa gttataccat aaagtactag actatttaga taaaaaagat
     9481 gaactatacg tatttaaagg ctacgctggt agcgataaag atacaatgtt aaaacttaca
     9541 gtcatcaatg aattagcatg gcataattta tttgctaaaa atatgtttat tagacctgaa
     9601 tcaaaagaag aagctacaaa gattaaacct aacttcacta tcgtttctgc cccacatttt
     9661 aaagcagatc cagaagttga tggtactaaa tctgaaacct ttgtcattat ttcatttaaa
     9721 cacaaagtca ttttaatcgg cggtactgaa tacgctggtg aaatgaaaaa aggtatcttc
     9781 tctgtaatga attatctctt accgatgcaa gatattatga gcatgcattg ctcagcaaac
     9841 gttggtgaaa aaggcgatgt tgcattattc tttggtctat ctggcactgg taaaacaacc
     9901 ttatcggctg atccacaccg aaaactaatc ggtgatgatg aacacggctg gaataaaaac
     9961 ggggtcttta atatcgaagg tggctgctat gcaaaagcaa ttaatctttc caaagaaaaa
    10021 gaaccacaga tttttgacgc aatcaaatat ggtgcgattt tagagaacac tgtagttgca
    10081 gaagatggtt cagtggactt tgaagacaat cgttatacag aaaacactcg tgccgcttat
    10141 ccaattaatc acattgacaa tattgtagta ccatcaaaag cagcacatcc aaatacaatt
    10201 attttcttaa ctgcggatgc atttggtgtt attccaccga tttcaaagtt aaataaagac
    10261 caagcaatgt atcatttctt gagtggtttc acttctaaat tagctggtac agagcgtggt
    10321 gtgacagaac ctgaaccatc attctcaaca tgtttcggag caccgttctt cccgttacac
    10381 cctactgttt acgctgattt attaggtgaa cttatcgatt tacatgatgt tgatgtttat
    10441 cttgttaata ctggatggac tggcggaaaa tatggtgtag gacgtagaat cagcttacat
    10501 tacacacgtc aaatggtaaa ccaagcgatt tctggcaaat tgaaaaatgc agaatataca
    10561 aaagatagta cgtttggttt aagcattcct gtaaaaattg aagatgtacc gaaaacaatt
    10621 ttaaatccaa ttaatgcttg gagcgacaaa gagaaatata aagcacaagc agaagattta
    10681 attcaacgtt ttgaaaagaa cttcgaaaaa tttggtgaaa aagttgaaca tattgctgaa
    10741 aaaggtagct tcaacaaata aatttgaata ctaattaaag agccaaccgt tgttatagtc
    10801 taacaatggt tggctcctct tattttatgt gctaaaaatt tataggcaat tttattacaa
    10861 caatgcacat ttaaggtgac cttcatgcca aactcgcatc actcatttaa tggaagcagc
    10921 acgtcttcac ataaagtacc ggttccttac gctatgcatg ttgccctact ttgcataaag
    10981 tacttatcgt taactcaacg caagttgttc ctattcacaa aaagcacata cagttatctg
    11041 catggagcac attttcattg aaagcgcata cccctcattc aacgtatatc acacatcttc
    11101 aaagcttgat agttcccatg cgcacaccac gtttcatact agctatgcaa ctcaacttgg
    11161 ttcataaact ctttaatata agtcaatgtt tcaaccatcg ctggtggtct tggcacatgt
    11221 ccttctgcca tttgataaaa tgtttcatgc gtggcacctt ttaactctag ttggtccgct
    11281 aaataatacg catgatgaat accaacttgc tggtctttcc ctccatgtac aattaatatt
    11341 ggcggactgt tttcattaat gtttggaatc gcttggcgtg cctcatatgc cgctcgatct
    11401 tttttcggat gaccaatcat tcttcgtagc atgcctctta aatcgacacg ttcttcatac
    11461 attaaatcaa tatctgagac accaccccag attgtataac ttgttactgg taagtcttga
    11521 aatgtcaaca atccttgtaa accacctcgc gaaaaaccaa ccatgtggat aaatgcatgt
    11581 ggatatttat catgtagcaa ccttaataat tgcgtcacat catttaaatc gccacggtaa
    11641 aattcgtctt taccttcact cccattgtta cctcggtagt atggcccaat cactaaagtt
    11701 tgactatctg aaaattgcat taatctacct gcgcgcacac gtcctacttg acctttgcca
    11761 cctcgcaaat atactacaat gcgatttact tcatgatgtg gtgtcatcat taaagctttt
    11821 acttgtaagt catctgacaa atatgtaatt tcttcgaatt gatgcgtaaa agattcaatt
    11881 ggcattcgtt tacgtttgat aaaacccaag tgattgcacc ctctctacgc attttaaaat
    11941 ggtactatct tgcagtaaga aactccgttg tgcgagttca atatcattga tacagttaaa
    12001 caacactggc cctgctgttt ctaaataatc gttcttgctt accaatgatt caacttcgat
    12061 aaaatataca tcttttacaa aatcagtttg atcatgtgtt tcaatggtat attgtgctat
    12121 gtaatgaata tttttaactt tggcgcctgt ttcttcatat aattcacggg taactgcttc
    12181 agcactactt tccccgcgtt cccttttacc accaggaaat tcaatacccc gtaaattatg
    12241 tttggtaaaa agcaattgat ttttaaacgt tggaatagct agcacatgat tgccatctgc
    12301 tatctcatta tcctttttaa atgtcaaatt aacttgacga ttatctttat ccctaaactt
    12361 cacgcgcatc acatccctac attgtatgtt aatataatag ttaattacta tcgttggagg
    12421 cattaattat gaaaaagata ttcttggcga tgattcattt ttatcaacgt ttcatttcgc
    12481 cactcactcc accaacttgt cgtttttatc caacatgttc agagtacact agagaagcga
    12541 ttcaatacca cggtgctttc aaaggccttt atttaggtat ccgtcgtatt ttaaaatgtc
    12601 atccgcttca taaaggtggc tttgaccctg ttccgttaaa aaaagacaag tcagcaagca
    12661 agcattcaca taaacataac cattaatatg gttgtaattg agttatatcc actaaagggg
    12721 ggcgaaattc gagtcgcccc tcttttaata tgcctgaatg cgccaccaca tcttgttcaa
    12781 aataataacc tgctggtgta acatctcctg gataatcacc tttacgagca agcatcgctg
    12841 taaaataacg gcttaaacca tattcgtaca tgccgccaat aaccactttt acaccatgac
    12901 ttttcaaagt atcaattgcc gtttgcactt tatcaatgcc acctagacga aatggtttta
    12961 atacaacaac tttcacattg tataattcta tcaaattaat tatgtccaac aacgatgttg
    13021 ccttttcatc aagggctatt ggaggtattg ttccatccac tacttcatca agcatggaga
    13081 tatctttaaa tggctcttcg atataaagaa cctgttcacg cgctaataac tgtaactgtg
    13141 tgaaatcttg acgatccaag gactcatttg catctataac caattgaaag tgaaactcta
    13201 attcccgtaa cactctaatt tgatgcatga tttgaggtgt ccattttaat ttaattctgg
    13261 ccggcttagt tgcttttaat gactctagtt gtttatttga taagccgctc actgtcgctc
    13321 catatgctac tgaaaatgaa ggcagtacat gaaacatttg atacaatgcc atgacaatag
    13381 ttgcccttgc agcaggcgta ttttccaatg aatctactaa ttttagtgct gcttcatacg
    13441 tttcaaatga tttatttcta ttatcttcga accattgctc aattacatgt ttcactgaag
    13501 caattgtttc atgatcatac caatctgttt gaaaagcgtt acattccccg aaatatgcat
    13561 ttcctttatc atcaatcaat tcgataaaca aacaatcacg atgtgttaaa gtgactttcg
    13621 gtgttacaat ttgtgactta aatggctcac tatatttata aaaatgcaaa gctgtcaact
    13681 tcatcaaatc atcctctata caacttattt ctttgtaatt tacctgttga tgtataaggt
    13741 aaagtatcaa ccttttcaaa gtgtttcggt actttatatt tcgctaaatg ttgtgataaa
    13801 tatgcaatca attgtgcctt tgaaatgtca ctttcactga caaaatataa tttaggcact
    13861 tgtccccaag tatcatcagg atgccctaca catactgcgt cactgatacc tggaaattgc
    13921 ttcgctaccg tttcaatttg atatggataa atattttcac cgccactaat aattaaatct
    13981 ttacgtcggt cataaatcat gacataacct tcatgatcta tttcagcaat gtcacccgta
    14041 ttaaaataac cattttcaaa cgtacccgtt aaatctgttg gatacaaata tccattcatc
    14101 acattggcac ctttaatcat taattctcca tgaccttctt tattaggatt tttaatttta
    14161 acgtctacat tggcacttgg catccctaca gtgtcaggac gtgcatgcaa catttccggt
    14221 gttgctgtta aaaattgtga acatgtctca gtcataccaa atgaattata aattggcagg
    14281 ttatattgta atgccgtctc tatcaaagtg gcagataatt tagcaccgcc gagtaatatt
    14341 ttttgcaaat tataaggttc atgtaaacct tgttgcataa gccaatttaa agtttgtggc
    14401 acaagcgaaa tgtgcgtgat gcgttcattt ttaattatcg ttaaaatttg ttcggcattg
    14461 aatttatcaa caatgcgcac agtaaaccct tcaataacag ctcttaaaag tacactgaga
    14521 cccgaaatat gataaatcgg caagacagat agccaattag tatcacgatc aaatcccaag
    14581 ctctctttac atccgattgc actggcataa tgattacgaa acgtttgcgg caccgctttt
    14641 tgagggccag ttgtccctga tgtaaacata atcgatgcaa tgtcatctaa attaaatgaa
    14701 gtatttaata tgttggacgg cgactctttc ggcaccacag tttcattcga tgtatcatat
    14761 tggatatcca ttgtgttgtc caacaaaccg ttcattgtaa tatccgttcc agcgaattca
    14821 atatcatcca gcgatacaat ttgaaaccct cgcaattcca gtggcaaggt acaaaaaatc
    14881 aattgtacat cgattgacct catctgattc ttcatctcat taggtgtcaa ccttgtatta
    14941 atcatcgcaa tttcaatatt tgccaaccaa caagcatgta ttaaaatgat cgattgaatc
    15001 gaattatcta tgtatagccc gacacgagat tgttgataag ccttgagtct tttagccaat
    15061 agactcgctt cacagtataa attttgataa gtataagatt cttgaccgtc tgttatcgca
    15121 atatgatgtc cattttgttg tgcttgttta tataaccaaa agtccatacg ctattcctcc
    15181 aaattcatct accttataat tataacgatt ttatgacatt ctagcagtgg ttatgtttaa
    15241 aaatataaaa aagtagacga attgatgcat tgatatgatt gttataatgc tcaatacata
    15301 tcgttatatc attcgtctac tattatcagt tatttttatt taattttagt gtcattctgt
    15361 cattttgatg tggtgattta cccattgttg ccacatcatc tgcaatgtca attggtatac
    15421 gattcatgtc ttgtaataca cttaaatgga atacttcatc atctaaattt tcaatgagat
    15481 atacataata tgttatctta tcctttttat attttaacgt tttccaaaag tccgacttgc
    15541 aattcaatac attatccgga atatattcaa taaataagta acgtttgctg cctactttgt
    15601 ctacgaaata ttttacagtg cctttttcta tacctcttat atgtgcatag tctgctgaaa
    15661 agtaaatact acctattgtt tcattatgtt gttgtatttc aaatcgttgg cctactattt
    15721 tattatttgt gccacaacta cttaaaaaaa tcagtagata tagcattaaa catattttca
    15781 tccccttgaa ttttaaagac tttttcaaaa gcaacacctc taaaaataaa tacaatatat
    15841 tatatcaaat agaaattatt attttgttaa attatgttgt catgttcgtg tcattttgag
    15901 tcgtaataca actt
//
LOCUS       DAMZKC010000048        15817 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.048, whole genome shotgun sequence.
ACCESSION   DAMZKC010000048 DAMZKC010000000
VERSION     DAMZKC010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 15817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15817
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.048"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(135..254)
                     /locus_tag="POB55_001161"
     CDS             complement(135..254)
                     /locus_tag="POB55_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500133.1"
                     /translation="MAEQSKQKQANEQQKAQNLFARWRQLQNSNSESSNDTNK"
     gene            complement(328..801)
                     /locus_tag="POB55_001162"
     CDS             complement(328..801)
                     /locus_tag="POB55_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4930 family protein"
                     /protein_id="HDI6500134.1"
                     /translation="MRLIFSMIKNIIAVIAIVIIVYIALKYAPFLKEQDWNPVNHDND
                     QMNQVTQPTNDAQHVYVSGEKYSLKENDLIKNVPLSQIKNVFKMIDKQEFMAVSGMNR
                     MAYNDQYIIGQRGDEFILYKFGDESMRVYNTEFEMQQDLNELGQNLQLKPENAYQ"
     gene            961..1158
                     /locus_tag="POB55_001163"
     CDS             961..1158
                     /locus_tag="POB55_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500135.1"
                     /translation="MSILTIILIALLVILLFRVGLSILRFLIYVGLVLLCIYLGYQGL
                     IWLLDFFQINSGFLPHFQFNN"
     gene            complement(1275..1559)
                     /locus_tag="POB55_001164"
     CDS             complement(1275..1559)
                     /locus_tag="POB55_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500136.1"
                     /translation="MTLFIIIGVLVPMVYTMQLNIKNEPVTKRNLLITLALSTLGILV
                     TALAGVIVTKQAFPLLSVAIGSIFTGIVWGLLLSGSYALIRFLSNAFGRK"
     gene            complement(1627..2313)
                     /gene="radC"
                     /locus_tag="POB55_001165"
     CDS             complement(1627..2313)
                     /gene="radC"
                     /locus_tag="POB55_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HDI6500137.1"
                     /translation="MKIKEMVTSEMPRERLLSHGAKSLSNTELLAILINTGRKGFSSI
                     DISNELLKSASNLNELKKSSINDLIKVKGIGLQKAITLKAAFELGERMGRRAENNRIK
                     ITQPSDVADYMIPTMKDLTQEHFVILLLNSKNVVIKETCVFKGTLNSSIVHPREIFSI
                     AVRENANAIIAVHNHPSGDVTPSQEDIITTMRLKECGLILGIDLLDHIIIGDNRFTSL
                     VEAGYFDEND"
     gene            complement(2310..3017)
                     /locus_tag="POB55_001166"
     CDS             complement(2310..3017)
                     /locus_tag="POB55_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDI6500138.1"
                     /translation="MVVLLSYSCSCIFSFLYQFISIEETSFDYLHRRSKCDYCNSSLK
                     WYELMPIISFLLLKGRCRNCRKRISLTHFLGETFALIPIVFIKYDFTYVNATLFITTY
                     VFLLIFTMTDITSLMLDCRLIIIYCIVSLSLSMIYPVAFIIISMTTHIFYFLFRAYIG
                     YGDVLLISALSLFFPLQFTIYVILFTFVIAGLVALITMIFKPIKLLPLVPFIFISFFI
                     NSLFYNDIHQFLGGVYF"
     gene            complement(3287..4558)
                     /locus_tag="POB55_001167"
     CDS             complement(3287..4558)
                     /locus_tag="POB55_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDI6500139.1"
                     /translation="MNYLESLYWIHERTKFGIKPGVKRMEWMLAQFNNPQINIKGIHV
                     GGTNGKGSTVAYLRTALVENGYEVGTFTSPFIETFNERIGLNGVPISNDAIVELVSRI
                     KPVSEMMERETDLGVATEFEIITAMMFLYFGEIHPVDFVIVEAGLGIKNDSTNVFTPV
                     LSILTSIGLDHTDILGGTYLDIARDKGAIIKPNVPVIYAVKNEDALKYVRERAIEQHA
                     KPIELDREIVVVSQNDEFTYRYKDYELETIILSMLGEHQKQNAALAITALIELNEQGL
                     IELDFNKMIDGIESVRWTGRIEQVHDKPLIILDGAHNSESIDALIDTIKQYHDKEKVD
                     ILFSAINGKPINEMVKHLSLIAHTFYATEFDFPKALRKEEIVGSIENDEIQLVDDYVE
                     FIKNYQGDTLVITGSLYFISEVKSKLDFEAL"
     gene            complement(4571..7201)
                     /locus_tag="POB55_001168"
     CDS             complement(4571..7201)
                     /locus_tag="POB55_001168"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDI6500140.1"
                     /translation="MEMKPKYDPREVEAGRYEEWVKNGYFKPSEDKSKETYTIVIPPP
                     NVTGKLHLGHAWDTTLQDIITRMKRMQGYDTLYLPGMDHAGIATQAKVEAKLNEQGIT
                     RYDLGREKFLEQAWDWKEEYASFIRAQWAKLGLGLDYSRERFTLDEGLSKAVKKVFVD
                     LYNKGIIYRGERIINWDPKARTALSDIEVIHEDVQGAFYHFKYPYADGEGFIEIATTR
                     PETMLGDTAIVVNPNDERYKDVIGKTVILPIVGRELPILADEYVDIDFGSGAMKVTPA
                     HDPNDFEIGQRHQLENIIVMDENGKMNDKAGKYEGMDRFDCRKQLVKDLKEQDLVIKI
                     EDHVHSVGHSERSGAVVEPYLSTQWFVRMEDLAKRSLDNQKTDDRIDFYPQRFEHTFN
                     QWMENIRDWTISRQLWWGHQIPAWYHKETGEIYVGEEAPTDIENWQQDEDVLDTWFSS
                     ALWPFSTLGWPDLESEDFKRYYPTNALVTGYDIIFFWVARMIFQGLEFTDRRPFNDVL
                     LHGLVRAEDGRKMSKSLGNGVDPMDVIDEYGADSLRYFLATGSSPGHDLRYSTEKVES
                     VWNFINKIWNGARFSLMNIGEDFKVEDIDLSGNLSLADKWILTRLNETIATVTDLSDK
                     YEFGEVGRALYNFIWDDFCDWYIEMSKIPMNSNDEEQKQVTRSVLSYTLDNIMRMLHP
                     FMPFVTEKIWQSLPHEGDTIVKASWPEVRESLIFEESKQTMQQLVEIIKSVRQSRVEV
                     NTPLSKEIPILIQAKDKEIETTLSQNKDYLIKFCNPSTLNISTDVEIPEKAMTSVVIA
                     GKVVLPLEGLIDMDKEISRLEKELAKLQSELDRVDKKLSNENFVSKAPEKVINEEKRK
                     KQDYQEKYDGVKARIEQLKA"
     misc_binding    complement(7245..7546)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            7604..8164
                     /locus_tag="POB55_001169"
     CDS             7604..8164
                     /locus_tag="POB55_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HDI6500141.1"
                     /translation="MNECAFGTKDPVYLNYHDHVWGQPLYDSKALFKLLALESQHAGL
                     SWLTILKKKEAYEEAFYDFEPEKVAQMTAQDIDRLMTFPNIVHHRKKLEAIVNQAQGY
                     LKIEQAYGSFSKFLWSYVNGKPKDLQYEHASDRITVDDTATQLSKDLKQYGFKFLGPV
                     TVFSFLEAAGLYDAHLKDCPSKPKHN"
     gene            complement(8387..9460)
                     /locus_tag="POB55_001170"
     CDS             complement(8387..9460)
                     /locus_tag="POB55_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDI6500142.1"
                     /translation="MTMIYRNNFIVFVLSFFISILLYSSHVLLPFMFGPIIASIICVK
                     VFKLDIKWPFLLSELGIVLLGVQIGSTFTKNVVMDIKDNWLSIIVVSISILLIAIVMA
                     FIFKKIARINTETAILSVIPGALTQMLVMAEQDKRANLLVVSLTQTSRIIFVVVLVPF
                     ISYFFHDGNMHANGKLTKVLPLSQVLNIGQIVILAIAIFIVYLIMSKIKFPTFQLLAP
                     LIVLIVWNFSTGLTFTLDHWLLNMAQLIYMIRIGVQIAHLLSDLKGRLAIAITIQNIM
                     LIIGALIMVYVIHFFDNNPINELFLGAAPGGVNQIVLVALATGADVAMITSYHIFRIF
                     FILFIIAPGINYFLKYRSNKRED"
     gene            complement(9494..9595)
                     /locus_tag="POB55_001171"
     CDS             complement(9494..9595)
                     /locus_tag="POB55_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500143.1"
                     /translation="MGGDLIVKVHICFVVKTASGYCYLNKREAQAAI"
     gene            complement(9656..10942)
                     /gene="hemL"
                     /locus_tag="POB55_001172"
     CDS             complement(9656..10942)
                     /gene="hemL"
                     /locus_tag="POB55_001172"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HDI6500144.1"
                     /translation="MRYTKSEEAMKVAETLMPGGVNSPVRAFKSVDTPAIFMDHGKGS
                     KIYDIDGNEYIDYVLSWGPLILGHRDPQVISHLHEAIDKGTSFGASTLLENKLAQLVI
                     DRVPSIEKVRMVSSGTEATLDTLRLARGYTGRNKIVKFEGCYHGHSDSLLIKAGSGVA
                     TLGLPDSPGVPEGIAKNTITVPYNDLDALKIAFEKFGDDIAGVIVEPVAGNMGVVPPI
                     EGFLQGLRNITTEYGALLIFDEVMTGFRVGYHCAQGYFGVTPDLTCLGKVIGGGLPVG
                     AFGGKKEIMDHIAPLGNIYQAGTLSGNPLAMTSGYETLSQLTPETYEYFNMLGDILED
                     GLKRVFAKHNVPITVNRAGSMIGYFLNEGPVTNFEQANKSDLKLFAEMYREMAKEGVF
                     LPPSQFEGTFLSTAHTKEDIEKTIQAFDTALSRIVK"
     gene            complement(10990..11964)
                     /gene="hemB"
                     /locus_tag="POB55_001173"
     CDS             complement(10990..11964)
                     /gene="hemB"
                     /locus_tag="POB55_001173"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001633925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HDI6500145.1"
                     /translation="MKFDRHRRLRSSATMRDMVRENHVRKEDLIYPIFVVEKDDVKKE
                     IKSLPGVYQISLNLLESELKEAYDLGIRAIMFFGVPNSKDDIGTGAYIHDGVIQQATR
                     IAKKMYDDLLIVADTCLCEYTDHGHCGVIDDHTHDVDNDKSLPLLVKTAISQVEAGAD
                     IIAPSNMMDGFVAEIRRGLDEAGYYNIPIMSYGVKYASSFFGPFRDAADSAPSFGDRK
                     TYQMDPANRLEALRELESDLKEGCDMMIVKPALSYLDIVRDVKNHTNVPVVAYNVSGE
                     YSMTKAAAQNGWIDEERVVMEQMVSMKRAGADMIITYFAKDICRYLDK"
     gene            complement(11967..12635)
                     /locus_tag="POB55_001174"
     CDS             complement(11967..12635)
                     /locus_tag="POB55_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HDI6500146.1"
                     /translation="MKPVVVMTQTNDMQSDLVSIIHKPFIDIKPLNFDIHLLNQRYDW
                     LIFSSKNAVKFFYKYLKGINVDNIAVIGSKTAQYCESLGIRVDFMPNDFSQEGFLKSF
                     NQTNQKILLPSSELARPLLLAALSKDNEVVKIDLYTSVPNKQNIQDVKEMIEHQQIDA
                     LTFSSSSAVRYYFNEGFVPKFKSYFAIGEQTARTIKSYQQPVTIAEIQTLESLIEKIL
                     ESRG"
     gene            complement(12657..13583)
                     /gene="hemC"
                     /locus_tag="POB55_001175"
     CDS             complement(12657..13583)
                     /gene="hemC"
                     /locus_tag="POB55_001175"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HDI6500147.1"
                     /translation="MRKLVVGSRRSKLALTQSQQFIDKLKAVEPNLEIEIKEIVTKGD
                     RIVDKQLSKVGGKGLFVKEIQHELFEKNIDMAIHSLKDVPSVIPEGLTLGCIPDRELP
                     FDAYISKTHTPLSQLPEGSIIGTSSLRRGAQILSKYPNLEIKWIRGNIDTRLEKLQTE
                     DYDAIILAAAGLRRMGWSDDIVTSYLDRDTLLPAIGQGALGIECRSDDEELLTLLSKV
                     HNDEVAKCVTAERTFLAEMDGSCQVPIAGYATISDQKEIEFTGLIMTPDGKERFEYTM
                     NGTDPVELGKTVSNKLKEQGAYEIIKRLNEQH"
     gene            complement(13625..14440)
                     /gene="ccsA"
                     /locus_tag="POB55_001176"
     CDS             complement(13625..14440)
                     /gene="ccsA"
                     /locus_tag="POB55_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HDI6500148.1"
                     /translation="MQENLFIRFNEIILLIYLISIICYFYDFVQKSHKIRSLGIYLLG
                     IVWVLQTISLSIFIIQTRHIPLGSISDVFYTLSWLIISISLILNLIKVLNFSVFFLNL
                     IGLTLLGMNTFQPTHYTNKVQKIAVVDELLLVHIGLAVLSYVFFALAFVNALLYIMQY
                     RNLKEKRFDQKYFRIGSVATLESIVFYSTLSGWIILIFSIILGTQWGVISVGERIFID
                     PKVILSSIITVLYGSYILLRINKWLNSRYLIYYNIILFCLNMINLFFATHFVN"
     gene            complement(14462..15808)
                     /locus_tag="POB55_001177"
     CDS             complement(14462..15808)
                     /locus_tag="POB55_001177"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HDI6500149.1"
                     /translation="MHFIAISINHRTADVALREQVAFRDDALRIAHEDLYETKSILEN
                     VILSTCNRTEVYAVVDQIHTGRYYIQRFLARAFGFEVDDIKAMSEVKVGDEAVEHLLR
                     VTSGLDSIVLGETQILGQIRDAFFLAQSTGTTGTIFNHLFKQAITFAKRAHNETDIAD
                     NAVSVSYAAVELAKKVFGKLKSKQAIIIGAGEMSELSLLNLLGSGITDITVVNRTIEN
                     AMKLAAKHQVKYDELSSLPNLLESADIVISSTSAQSYIITNEMIERIAENRKQDSLVL
                     IDIAVPRDIEPGISAITNIFNYDVDDLKGLVDANLRERQLAAATISEQIPAEIHAHNE
                     WISMLGVVPVIRALREKAMAIQAETMDSIDRKLPGLSERERKIISKHTKSIINQMLKD
                     PIKQAKELSSDKKSNEKLELFQNIFDIEAECPHEQAKQQKESKVKEISARRIFSFE"
BASE COUNT         5154 a         2977 c         2174 g         5512 t
ORIGIN      
        1 gaaataaagc actaactacg aattactttg aaaatgaaca actccgtaaa aaatttgtgg
       61 cattttaaaa acaaaaaact actagcaaac attccagtca ctagtagttt aaataatcga
      121 tttaaatttt aaaattattt gtttgtatcg ttcgaggatt cactgttcga attctgaagt
      181 tgtctccaac gtgcgaataa gttctgggcc ttttgttgtt cattagcttg tttctgtttt
      241 gattgttctg ccatttgaac tacaccaacc ttttaaattt attctagtga caggataact
      301 aaaatatatt tcttatgcaa taatttccta ttgataagca ttttcgggtt ttagttgtaa
      361 attttgccct aattcattta agtcttgttg catttcaaat tcagtattgt aaacacgcat
      421 tgactcatct ccaaatttat aaagaataaa ttcgtctcct ctttgaccta taatatattg
      481 atcattataa gccatgcgat tcattccaga cacagccata aactcttgtt tatctatcat
      541 tttaaataca tttttaattt ggcttaatgg tacattcttt attaaatcat tttcttttaa
      601 tgaatatttc tcaccactta cataaacatg ttgggcatca ttagtgggtt gcgtaacttg
      661 attcatttga tcattatcgt gatttacagg attccaatct tgttctttta aaaaaggtgc
      721 gtatttcaag gcgatataaa caattatcac aattgctatt actgctataa tgtttttaat
      781 catactaaat atcaatctca tagttcgcct ccaatgtctt catctaatta tatattttat
      841 tatgtatatt ttcctcagtc ttcagcctta atcatgatat atcttaggac tgatgcacgt
      901 tcatttaaaa tagcttataa ttttgttatg atttaaaagc gtatgaggag gtttgtagca
      961 ttgagtatac taacaattat tttaatagca ctattagtca tcctcctttt tagagttgga
     1021 ctttctattt tgcgattttt aatttatgtc ggacttgttt tattatgtat ttacttagga
     1081 tatcaaggtt taatttggtt gctcgatttt tttcaaataa atagtgggtt tttaccgcac
     1141 tttcaattta acaattaaat ttattctgca taacaaaact cctaatgtac tagtttagaa
     1201 ttttttaact agttccacat taggagtttt aatttggttt caagcacgga tactacttga
     1261 tgaacagatt actattactt acgcccaaat gcgttagata aaaatcgtat cagcgcgtag
     1321 ctaccactta gtaaaaggcc ccaaacgatt ccagtaaaaa ttgagccgat tgctacactt
     1381 aataaaggaa aagcttgttt cgtaacgatc acacctgcta acgccgttac taaaatacct
     1441 aacgtagata aagctaatgt tattaataga ttgcgctttg ttacaggttc atttttaata
     1501 tttaactgca tggtataaac cattggcacg agaaccccga taataataaa tagagtcact
     1561 atttttcctc caatgaaagt acttcgtagg tattatacac aagatacgtc attaattcta
     1621 tcaacttcaa tcattttcat caaagtaacc cgcttctaca agactggtaa atctattatc
     1681 accgattata atatgatcca ataaatctat ccctaaaatc aaaccacact ccttcaacct
     1741 cattgttgtt atgatatctt cttgtgaggg cgttacatca ccggatggat gattatgaac
     1801 tgcgatgatt gcattggcat tttctctcac cgcaatacta aaaatttcac gtggatgtac
     1861 aatcgaacta tttaatgtac ctttaaaaac acaggtttct ttaatcacta cattttttga
     1921 atttaacaat aaaatgacaa aatgttcttg tgttaaatct ttcattgttg gaatcatata
     1981 atcagcaaca tcacttggtt gcgttatttt tatacgatta ttttcagctc ttctccccat
     2041 cctttcccct aactcaaatg ctgcttttaa agtaattgct ttttgtaatc caatcccttt
     2101 aacttttatc aaatcgttaa ttgaagattt tttcaattca ttcagattcg aagcagattt
     2161 aagcagttca ttactaatgt ctatgctcga gaatcctttt cttccggtgt taattaatat
     2221 agctaataat tctgtattcg aaagactttt tgcaccatgg cttaacaaac gttctcttgg
     2281 catttctgaa gttaccattt ctttaatttt caaaaatata cgcctcctaa aaattgatgg
     2341 atatcattat aaaaaagtga attgataaaa aaggaaataa atataaatgg aacaaggggt
     2401 aatagtttaa tcggcttaaa tatcatggta attaaagcaa ctaaaccagc aatgacaaat
     2461 gtaaataaaa tgacataaat agtgaattgg agagggaaaa acaaagaaag tgcagatatt
     2521 agtaaaacgt caccataacc aatatatgcc cgaaataaaa agtagaatat atgcgtggtc
     2581 atactaataa tgataaaagc tactggataa atcatactta acgagagaga aacgatacaa
     2641 taaattataa ttaagcgaca atctaacatt aaagaagtga tatcggtcat agtaaaaata
     2701 agcagaaaaa catatgtagt tataaatagc gtagcattta cgtatgtgaa atcatactta
     2761 ataaagacga taggtattaa agcaaaggtt tcccctaaga aatgtgttag ggaaatacgc
     2821 tttcgacagt ttcgacatcg cccttttaat aataaaaaac taataatcgg cattaattca
     2881 taccatttga gtgacgaatt acaataatca catttcgatc ttctatgtaa ataatcaaat
     2941 gacgtttctt ctatagatat aaattgatat agaaaactaa aaatacaact gcaactataa
     3001 gataacaata ctaccaaatg acaacctcct tatgtaaatt atagttagtt attaccaaaa
     3061 tgtaaatata cactattttt caagaattga accgcttttt catttaaatt tttcaatatt
     3121 gctaagcata attgatggat actttaacaa cccattactg ctcggcaaaa ttaataatgg
     3181 caagaaattg aaccttataa acacatacga tttagagcat aaaaaataac catgaagctc
     3241 tacctattga ttaaatagat tcttcatggc tattttagtt ttagttttat aatgcttcaa
     3301 agtctaattt tgatttaact tcacttatga aatacagact accggtaatt actaatgtat
     3361 caccttgata attttttata aattcaacgt agtcatctac taattgtatt tcatcatttt
     3421 caatactacc tacaatttct tctttgcgta acgctttcgg aaaatcaaat tcagttgcat
     3481 aaaacgtatg cgcaattaaa cttaaatgtt tgaccatctc gttaatcggt tttccgttta
     3541 ttgctgagaa caaaatatct actttttctt tatcatggta ctgtttaatt gtatcaatta
     3601 gagcatctat actctctgaa ttatgtgcgc catccaaaat gattaaaggt ttgtcatgca
     3661 cctgctcaat acgtccagtc caacgaactg attcaatacc gtctatcatc ttattgaaat
     3721 ctaattcaat taatccttgt tcatttaatt caataagagc tgttatggct aatgcagcat
     3781 tttgtttctg atgttcacct aacatgctta aaatgattgt ttctaattca taatctttat
     3841 aacggtaagt aaattcatca ttttgcgata caacaacaat ttctctatct aattcaattg
     3901 gctttgcatg ttgttcaatt gcgcgttcac gaacatattt taatgcatct tcatttttaa
     3961 cagcatatat cactggaacg ttaggcttta taatcgcgcc tttatcccta gcaatatcta
     4021 gataagtacc acctaaaata tctgtatggt ctagaccgat actagttaag attgataaaa
     4081 ccggtgtaaa gacatttgtc gaatcgttct ttatacccaa tccagcctca acaatgacaa
     4141 aatcaacagg atgtatttca ccaaaatata aaaacatcat cgctgtgatt atttcgaatt
     4201 cagttgcaac acctaaatct gtttcacgtt ccatcatttc acttactggt ttaatacgtg
     4261 atactaattc tacaatagcg tcatttgata ttggcacacc atttagacca attcgttcat
     4321 taaatgtttc aataaacggc gacgtaaatg tacctacttc ataaccattt tcaactaaag
     4381 ctgttctaag gtaagcaact gtagagcctt taccatttgt gccacctaca tgaataccct
     4441 taatgttaat ttgaggatta ttaaattgtg ctagcatcca ttccatacgt ttaacacctg
     4501 gtttgatgcc aaatttagtt ctttcgtgta tccaatacaa gctctctagg taattcattg
     4561 ttactaactc ctatgctttt aattgttcaa ttcttgcctt cacaccatca tatttttctt
     4621 gataatcttg ttttttacgt ttttcttcat ttataacctt ttcaggtgct ttacttacaa
     4681 agttttcatt agagagcttt ttatctactc tatctaattc gctttgaagt ttagctaatt
     4741 ctttttccaa acggctgatt tccttatcca tatcaattag cccttctaat ggtaatacca
     4801 ctttacctgc aattacaact gatgtcattg ctttctcagg aatttccacg tcagtgctaa
     4861 tatttaaggt actaggatta cagaatttga ttaaataatc tttgttttgt gataaagttg
     4921 tttcaatttc tttatcttta gcttgaatta aaataggtat ttctttagac aatggcgtat
     4981 ttacttctac acgtgattgt cttacagatt taatgatttc aacaagttgt tgcattgttt
     5041 gtttactttc ttcaaaaatc aatgattcac gcacttctgg ccatgaagct ttaacaattg
     5101 tgtcaccttc atgtggtaaa ctttgccata ttttctctgt tacaaatggc atgaatggat
     5161 gtagcattct cataatattg tctaaagtat aactcaatac tgaacgtgta acttgttttt
     5221 gttcttcatc attactattc attggaattt tactcatttc aatgtaccaa tcacagaaat
     5281 catcccaaat gaaattatat aatgcacgtc caacttcgcc gaattcatat ttgtcactta
     5341 aatcagtaac tgttgcaatc gtttcattta aacgtgttag aatccattta tctgctaatg
     5401 ataagttacc acttaaatcg atatcttcaa ctttaaagtc ttcaccgata ttcattaaac
     5461 tgaaacgtgc cccattccag attttattga taaagttcca cactgactca actttttcag
     5521 ttgagtatct taaatcatgt cctggagatg aacctgttgc taagaagtaa cgcaagctat
     5581 cagcaccgta ttcgtcaata acatccattg gatccacacc attacctaat gatttactca
     5641 tcttacgccc gtcttcagca cgaactaaac cgtgtaataa tacatcatta aatggacgac
     5701 gatctgtaaa ttctaagcct tggaatatca tgcgtgctac ccagaaaaag ataatatcgt
     5761 aacctgtaac taaggcattt gttgggtagt atcgtttaaa gtcttcactt tctaaatcag
     5821 gccaacctaa cgtagagaaa ggccataaag cacttgagaa ccacgtatct aatacatctt
     5881 catcttgttg ccaattttca atatcagttg gcgcttcttc tccaacatat atttcgcctg
     5941 tttctttatg ataccaagcc ggaatttgat gaccccacca taattgtctt gaaatcgtcc
     6001 aatctctaat attttccatc cattggttaa atgtatgttc gaaacgttgc ggataaaaat
     6061 caatacgatc atctgttttt tggttatcta atgaacgttt cgctaagtct tccatgcgca
     6121 caaaccattg tgttgataaa tatggttcaa caacagcgcc agatcgttct gaatgaccta
     6181 cagaatgaac atgatcttca atcttgataa ctaaatcttg ttcttttaaa tctttaacta
     6241 gctgtttacg acaatcaaaa cggtccatac cttcatattt acccgctttg tcgttcattt
     6301 taccattttc atccataacg ataatatttt ctaattgatg tctttgacca atttcaaaat
     6361 cattagggtc atgtgctggt gtcactttca tagcaccaga accgaagtct atatcaacat
     6421 actcatctgc taaaataggc agttcgcgtc ctacgattgg taatataaca gttttaccga
     6481 ttacatcttt gtatcgttcg tcattagggt taacaacaat cgctgtatca cctaacatcg
     6541 tttctggtct tgttgttgca atttcaataa aaccttcacc atcagcgtaa ggatatttaa
     6601 aatgataaaa cgcaccttga acatcttcat gtattacttc aatatcagat aaagctgtac
     6661 gtgctttagg atcccaattt ataatacgtt cgccacgata aataattcct ttattgtata
     6721 aatcaacaaa aactttttta actgctttac ttaaaccttc atctaaagta aaacgttctc
     6781 tactataatc taaacctaga cctaatttag cccattgcgc acgaataaat gacgcatact
     6841 cttctttcca atcccatgcc tgttctaaaa acttttcacg accaagatca tatctagtta
     6901 ttccttgttc atttaattta gcttctacct ttgcctgtgt cgcaatacca gcatgatcca
     6961 tacctggtaa gtataacgta tcgtatcctt gcatacgttt catacgtgta atgatatctt
     7021 gtaaagtcgt atcccatgca tgtcctaaat gtaatttacc agttacattt ggtggcggga
     7081 taacaattgt atatgtttct tttgatttat cttctgacgg tttaaaataa ccattcttta
     7141 cccattcttc ataacgtccc gcttcaactt cacgaggatc atattttggt ttcatttcca
     7201 tttgctatac ctcctaaaaa aataaaaata tccatcctat atacaaatag gacggatatt
     7261 ccgtggtacc acctatattc aagaaggatg tttaatatca aattcactct tttaacataa
     7321 ttggaataat cataccaata ctatcatcgt gaaatttgaa atgcttcatc tcttcaagca
     7381 ctctagatta tgattaacgc tcaaacacgt cttagcctac tattaatcac gttcagctaa
     7441 gatactctgt gggctacctt cagtaaaaat catttacata ctcacaccaa atcatatgct
     7501 ctctttaaaa taatttgaac ttactcttcc caaatcctat attaaactct taacttatag
     7561 tataatgatt gacaaaataa gtcaatgtat aggtgggaat aaaatgaatg aatgcgcatt
     7621 tggtactaaa gatccagtct acttaaacta tcatgatcat gtatggggac aaccgctcta
     7681 tgatagcaag gcattgttta aacttttagc attagaatca caacatgctg ggctatcttg
     7741 gttaactatt ttaaaaaaga aagaagccta tgaagaagca ttttatgatt tcgaaccaga
     7801 aaaggtagca caaatgaccg ctcaagatat cgaccgctta atgacttttc caaatatcgt
     7861 tcatcatcgt aaaaaattag aagcaattgt taatcaagct caagggtatt taaaaattga
     7921 acaagcatat ggtagtttta gtaaattttt atggtcttat gtaaatggta agcctaaaga
     7981 tttgcagtat gaacatgctt ctgatcgtat cacagttgat gatactgcaa cacaactatc
     8041 taaagattta aaacaatacg ggtttaaatt tttaggtcca gtaacagtat tttcgttttt
     8101 agaagcagct ggtttatatg atgcacattt aaaagattgt ccatcaaagc ctaaacacaa
     8161 ttaatttgct atagcatcat aaagtaaacg agacatcatc tatttttaac taatagactg
     8221 tctcgttttt tgatgtgaaa atttattcaa atcataagtt aaagttccaa gttaatgcac
     8281 tgctaatatt agatatctta atctaaaaat agatgtacaa cctttgctta aaccttaata
     8341 atgaattcta tcctaagcat aaatcaactt aaatggatga tagtttctaa tcttcacgct
     8401 tattcgaacg atattttaaa aagtaattaa ttcctggtgc aataataaat aggatgaaaa
     8461 agattcggaa aatatgataa ctcgtaatca tagcaacatc ggcaccagta gctaatgcaa
     8521 ctaaaactat ctgattaacc cctcctggtg ctgcaccaag aaacaattca ttaataggat
     8581 tattatcaaa gaaatgtatg acataaacca tgattagcgc accaattatc aacataatat
     8641 tttgaattgt aattgcgatt gctagtctac cttttaaatc tgacaataaa tgcgcaattt
     8701 gaactccaat tctaatcata tatattagtt gtgccatgtt caacaaccaa tgatctagtg
     8761 taaatgttaa acctgtagaa aaattccaaa caattaatac aatgagtggt gctaataatt
     8821 gaaatgttgg aaactttatt ttagacataa ttagataaac tataaagata gctatcgcta
     8881 aaataactat ttgccctatg tttaatactt gtgataaagg caagactttt gttaactttc
     8941 cattcgcatg catgttacca tcatgaaaaa aatatgaaat gaacggtact aaaacaacaa
     9001 caaatataat tcgtgatgtt tgcgttaagc taacaactaa caaattagca cgtttgtctt
     9061 gttcagccat gaccagcatt tgtgttagtg ctcctggtat aacacttaaa atagctgttt
     9121 ctgtattaat acgtgcaatt tttttaaaaa taaatgccat tactattgca attaataata
     9181 tcgaaataga tacaacaata atcgaaagcc aattgtcttt aatatccata acgacatttt
     9241 tcgtaaacgt tgatccgatt tgcacaccta atagtacaat acctaattca ctaagtaaga
     9301 atggccattt aatatcaagt ttgaaaactt ttacacaaat gattgatgcg ataataggac
     9361 caaacataaa tgggagtaat acgtgcgatg aatacagtag aatactaata aaaaatgata
     9421 aaacgaacac aatgaaatta tttctatata tcattgtcat gttttccact tctttcaata
     9481 aaaaataaaa tgactaaatt gctgcttgag cttcacgttt gttaagataa caatatccgc
     9541 tagcagtttt gactacaaag catatatgga ctttcactat caagtcgccg cccatgcctg
     9601 atatacattt aaaaagagcc tgaacaaagt tcaggctctc aatttgtccg tatatttatt
     9661 ttacaatacg acttaaagcc gtatcaaatg cttgaatcgt tttttcaata tcttctttcg
     9721 tgtgtgccgt agataggaat gtaccttcaa attgagatgg tggtaaaaac acaccttctt
     9781 ttgccatttc tcgatacatt tctgcaaata atttcaaatc acttttattc gcttgttcaa
     9841 aattagttac aggtccttca tttaagaaat aaccaatcat tgaacctgct ctatttacag
     9901 ttattggtac attgtgttta gcaaatacac gctttaaacc gtcttcaagt atatcgccta
     9961 acatattaaa atactcatat gtctctggcg ttaattggct taacgtttca taaccacttg
    10021 tcattgcaag aggatttcct gataacgtac ccgcttgata aatatttcct aatggtgcta
    10081 tatgatccat gatttctttt ttaccaccaa aagcacctac aggtagtcct ccaccgataa
    10141 cttttcctaa gcaagttaaa tctggtgtca caccaaagta accttgtgca caatgataac
    10201 cgactctgaa accagtcatt acttcatcga aaattagcaa tgcgccgtat tcagtcgtaa
    10261 tatttcttaa tccctgtaaa aaaccttcaa tcggcggtac gacacccata ttaccagcaa
    10321 caggttctac gattacacca gcaatatcgt ctccaaattt ttcgaaagcg attttaagtg
    10381 catctaaatc attgtatgga actgtaattg tatttttagc aataccttca ggcacaccag
    10441 gagaatccgg caatcctaat gttgccaccc cagaaccagc tttgattaat aacgaatcac
    10501 tatgaccatg atagcaacct tcaaatttca caattttatt tcttccagta taaccacgtg
    10561 ctaatcttaa agtatccaat gtagcttctg taccagatga caccatacgc actttttcta
    10621 ttgaaggtac tcggtcaata acgagctgcg ccaatttatt ttcaagtaat gttgatgcac
    10681 caaaacttgt acctttatca attgcttcat gtaaatgact aataacttga gggtctctat
    10741 gtcctaaaat aagtggcccc caacttagta catagtcgat atactcgtta ccatcgatat
    10801 cataaatttt tgaaccttta ccgtgatcca taaaaattgc tggtgtatct actgatttaa
    10861 atgcgcgtac tggactattt acaccaccag gcattaaagt ttcagcaacc ttcattgctt
    10921 cttctgattt cgtatatctc ataaagtaaa ttcctcctat agtttatgga aaatcataca
    10981 tataaaacct tatttatcta aatagcgaca aatgtccttt gcaaaatacg taataatcat
    11041 atcagcacct gcacgtttca ttgaaaccat ttgttccata acgacacgtt cttcatctat
    11101 ccaaccattt tgtgccgctg ctttagtcat actatattct ccactcacat tatatgcaac
    11161 aactggaaca ttcgtatgat ttttaacatc tcgaactatg tctaaataac ttagagcagg
    11221 tttaacaatc atcatgtcgc acccttcttt aagatcactt tctaattcac gaagtgcttc
    11281 caaacggtta gcagggtcca tctgatacgt ttttctatcc ccaaatgatg gcgctgaatc
    11341 tgctgcatct ctaaaaggtc caaagaaact tgatgcatac ttgacaccat aactcattat
    11401 aggaatattg taatagccgg cttcatctaa tccacgacga atttcagcaa caaaaccatc
    11461 catcatatta cttggcgcaa taatatcagc accagcttcc acttgagaaa ttgctgtttt
    11521 aacaagcagt ggcaatgatt tatcattgtc aacgtcatgt gtatggtcat caatcacgcc
    11581 acaatgacca tgatcagtat attcacataa acaagtgtct gcaacaatta ataagtcatc
    11641 atacattttt ttagcaatac gtgttgcctg ttgaataaca ccatcgtgaa tgtatgcacc
    11701 agtacctata tcatcttttg agtttggaac accgaaaaac ataatggcac gtatgcctaa
    11761 gtcataagct tcttttaatt cactttcaag taaattcaaa ctgatttggt atacacctgg
    11821 caatgactta atttcttttt tcacatcgtc tttttcaact acaaaaattg gatatattaa
    11881 atcttctttt cttacatgat tctctctaac catatctctc attgtcgctg atgatctcaa
    11941 tcttctatgt ctatcaaatt tcatttttag cccctacttt ctaaaatctt ttcaattagt
    12001 gattcgagtg tttgaatttc tgcaattgtt actggttgtt gatatgattt aatggtccgt
    12061 gctgtttgtt ctccaatagc aaaatacgac ttgaattttg gtacaaatcc ttcattaaaa
    12121 taataacgta ctgccgacga acttgaaaat gttaatgcat cgatttgttg atgttctatc
    12181 atttctttaa catcttgtat attttgtttg ttaggcactg aagtatataa atctatttta
    12241 acaacttcat tatctttaga taacgctgct aataacaatg gtctcgccaa ttcactcgaa
    12301 ggcaaaagta ttttttggtt agtttgatta aatgatttta aaaatccttc ttgagaaaag
    12361 tcgtttggca taaaatcaac tcgaatgcca agtgattcac aatattgcgc tgtcttactt
    12421 cctatcacag caatgttatc aacattaatt ccttttagat atttataaaa gaatttcaca
    12481 gcatttttag atgaaaaaat aagccagtca tagcgttgat ttaacaaatg aatatcaaaa
    12541 tttagtggct ttatatcaat aaagggtttg tgaataattg atactaaatc actttgcatg
    12601 tcatttgttt gtgtcataac tacaactggc ttcatattta aacgtcactc cattatttaa
    12661 tgttgttcat ttaagcgttt tataatttca taagcacctt gctcttttaa tttgttactc
    12721 actgttttgc ctaactcaac cggatctgtt ccgttcattg tatattcaaa tcgttcttta
    12781 ccatctgggg tcataattaa acctgtaaat tcgatttctt tttgatctga gattgtagca
    12841 tatcctgcga ttggcacctg acaactacca tccatttctg ctaaaaacgt tcgttcagca
    12901 gtcacacatt ttgcaacctc atcattatgt actttgctta ataatgttaa tagttcttca
    12961 tcgtcactac gacattctat ccctaaagca ccttgtccga ttgcaggtaa caatgtatct
    13021 ctatcaagat aagatgttac aatatcatct gaccagccca ttcttcttaa accagctgca
    13081 gctaaaataa tcgcatcata atcttcagtt tgtaactttt ctaatcgtgt atctatatta
    13141 cctctaatcc atttaatctc taaattagga tacttagata atatttgtgc accacgacgt
    13201 aatgaactag taccaataat actgccttct ggcaattggg atagtggtgt atgtgtttta
    13261 gaaatatacg catcaaaagg taattctcta tcagggatac aacctaatgt taaaccttcc
    13321 ggaattacac ttggtacatc tttaagcgag tgtattgcca tatcgatatt tttttcaaaa
    13381 agttcatgtt gtatttcttt aacaaataag cctttgcctc cgactttaga caattgctta
    13441 tctactatac gatcgccttt cgtgacaatt tctttaattt caatttctag atttggctcg
    13501 acagctttta atttatcaat aaattgctgg ctttgtgtta aagctaattt acttcttctg
    13561 gagccaacga ctaatttacg catgttcaat tcctcctagg aacggattgc tctagattat
    13621 tttctcaatt cacaaaatgt gttgcaaaaa ataaattaat catatttaag caaaataaaa
    13681 taatgttata gtatattaaa taccttgaat tcaaccattt gttgattcta agcaaaatat
    13741 aacttccata taatactgta ataattgaag agagtattac cttcgggtca atgaatatac
    13801 gttcaccaac tgaaattaca ccccactgtg tacctaaaat aatactaaat atgagaatta
    13861 tccacccact taacgttgag taaaacacaa ttgattcaag tgtagcaacg ctacctattc
    13921 taaagtattt ttgatcaaaa cgtttttcct tcaaattacg gtattgcatg atatacagta
    13981 atgcattgac aaaagctaag gcaaagaaga cataacttaa cacagctaga ccgatatgga
    14041 ctaacagtaa ctcgtctaca acagcaattt tctgaacctt attagtataa tgtgtcggtt
    14101 gaaatgtatt catccctaaa agtgttagcc ctattaaatt caagaaaaac acagagaaat
    14161 tcaaaacttt gataaggtta agaattaaag agattgaaat aatcagccaa cttaaagtat
    14221 agaatacatc tgaaatagac cctaatggaa tatgtctagt ttgtataata aaaatagata
    14281 aagagattgt ttgtaaaacc caaacaatcc ccaataaata tatgcctaaa cttctaatct
    14341 tatgactttt ttggacaaaa tcataaaaat agcaaatgat actgattaag tatattaata
    14401 atataatttc attgaatcga ataaacaggt tttcttgcat attatcacca tattaatatg
    14461 cttattcaaa actaaagata cgtcgcgctg aaatttcctt gaccttgctt tctttttgtt
    14521 gcttcgcttg ctcatgagga cattcagctt cgatatcaaa tatattttga aatagctcta
    14581 atttttcatt acttttctta tcactactta attctttggc ctgtttaata ggatctttca
    14641 acatttgatt gatgatactt tttgtatgtt tagatataat tttacgctct ctttcgctta
    14701 accctggcaa tttacgatca atactgtcca tcgtttctgc ttgaattgcc atagcttttt
    14761 cacgtaaagc tctaatcact ggaacaacac ccaacatact aatccactca ttgtgtgcat
    14821 gtatttctgc aggaatttgt tccgaaattg ttgcagccgc taattgtcgc tcacgtaagt
    14881 ttgcatcaac taaacctttt aagtcatcaa catcataatt aaagatgttt gtgatggcac
    14941 taataccagg ttcaatatct cgaggaactg caatatcaat caatactagt gaatcttgct
    15001 ttctattttc tgcaattctt tctatcattt catttgtaat gatataagat tgtgcactcg
    15061 ttgaactaat cacaatatct gcactttcaa gtaaatttgg taatgatgat agttcatcat
    15121 atttcacttg atgctttgct gctaatttca tagcattttc aattgttcta tttactactg
    15181 taatatcagt aattccagaa ccaagaagat ttaatagtga taattcactc atttcccctg
    15241 caccaataat gatagcttgc ttacttttca atttgccaaa tacttttttc gccaactcga
    15301 ccgcagcata agacacactt acagcattat cagctatatc tgtttcatta tgtgctcttt
    15361 ttgcaaaagt aattgcctgt ttaaatagat gattaaaaat tgttcctgtc gtacctgtgc
    15421 tttgcgctaa gaaaaatgca tctcttattt gacctaaaat ttgagtttct ccaagtacga
    15481 ttgaatctaa accagaagtg acacgcaata aatgttctac tgcttcgtcc cccactttta
    15541 cttctgacat tgctttaata tcatctactt caaatccaaa tgcacgagct agaaatcgtt
    15601 gaatatagta acgacctgtg tgaatttgat caacaacagc atatacttca gttcgattac
    15661 atgttgataa tatgacattt tctaaaatag atttagtttc atataaatct tcatgggcaa
    15721 ttcgtaaggc atcatctcta aaagcaactt gctctcttag tgcaacatca gctgtgcgat
    15781 gatttatact aattgcaata aaatgcattt gaaacgc
//
LOCUS       DAMZKC010000049        15489 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.049, whole genome shotgun sequence.
ACCESSION   DAMZKC010000049 DAMZKC010000000
VERSION     DAMZKC010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 15489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15489
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.049"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(334..1611)
                     /locus_tag="POB55_001178"
     CDS             complement(334..1611)
                     /locus_tag="POB55_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6500150.1"
                     /translation="MKEKRTNVRWMFALAFFFIGVIAYMDRANISYIAKQMMDDLGMT
                     KPQFGLLASFFSLGYALMQVPSGMLAEKFGPRKMITIALVWWSAFTILTGMIKNHGLI
                     YLVRFLFGVGEAPMYPSNAVFNSFWFSKNEKGRASSALLAGSYFGPVLAPIVTIAIVN
                     AFNWQAVFYIFGAVGILMAVLWAIIAKDLPEQHRMVNEAEKRFIMENRDIVATEKSSP
                     PWNDFFKRFSFYAIAIQYFVVQFIITLFLIWLPTYLTEVFHVNFKEMSISSLPWLLMF
                     FLILSAGAISDRVLGLGRSKFVARGVIAIAGFIVFAVSIIFAVRTGNLYVSIFWLSLG
                     LGGIGISMGMSWAAATDLGRNFSGTVSGWMNLWGNIGALISPLLAGLFVEHLGWTMTF
                     QLLIVPAVIAVIMWFYVKPDQPLIVSDDKAIEK"
     gene            2158..2769
                     /locus_tag="POB55_001179"
     CDS             2158..2769
                     /locus_tag="POB55_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDI6500151.1"
                     /translation="MEQIITEFISRFGYAAIFILILLENVLPIVPSEIILTFAGLMSV
                     KSHLSILTLFIIATIASFIGLLILYYICRLISEERLYRFIDRHGKWIKLKSKDLKRAN
                     DWFKKYGVWAVFICRFIPVLRVLITIPAGVNRMNVVTFTVISLIGTTIWNFGLILLGR
                     TLSDSFGMLMTGLHTYSRIMYVVIIIAVIYFAIRYIGKRKRVK"
     gene            3287..3982
                     /locus_tag="POB55_001180"
     CDS             3287..3982
                     /locus_tag="POB55_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDI6500152.1"
                     /translation="MDVLTIEHLTKKIGNKTILEDVSFKLKRGQIVGLVGANGAGKTT
                     LMKVILGYSSFQSGNFNVINSKDSKSNIGALIENPGIYPFMSGYENLKLLNESKNTQD
                     IDKIVSQLHMDEYIHKKAKTYSLGMKQKLGIAIAFLNEPQFIILDEPMNGLDPKAVRD
                     VRELIVQKAQEGVTFLISSHILSELVKITNSILIINKGKIVTETSEEELKQFKDNDLE
                     NVLLEIIEREDQA"
     gene            3984..4754
                     /locus_tag="POB55_001181"
     CDS             3984..4754
                     /locus_tag="POB55_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6500153.1"
                     /translation="MGTLIKQECFKLFKKKSTFIAPIVFILLMVAQGYIATKYNKIFT
                     PQESFTSAYNGFSWFAFLLIIQASTIISMEFHYGTIKNLLYREYSRTTMIVSKIITLF
                     IISLIYFVITIIASIVIGSLFFNDLNIFESSGNQLSLLNQLLLVSLGTFVGVWLVLSL
                     TLLLSSATNSTGVAIAVGIVFYFASSILAVIQTALLEKIDWLKWNPINMMNIMLQTVE
                     KGFSKSTKLELHELFIGNIAYISIFLILVVFIFKKKNI"
     gene            5128..7092
                     /locus_tag="POB55_001182"
     CDS             5128..7092
                     /locus_tag="POB55_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HDI6500154.1"
                     /translation="MTQITEKELKKKYLDLLSQNFDTPEKLATEIINLESILELPKGT
                     EHFVSDLHGEYEAFQHVLRNGSGNVRAKINDIFKERLSTKELNDLTALVYYPEDKLKL
                     IKSDFQSCGQLNVWYITTIEHLIELIKYCSSKYTRSKLRKALPKQYVYIIEELLYKSN
                     EYQNKKSYYETLVNQVIELKQADDLIIGLAYSVQRLVVDHLHVVGDIYDRGPQPDKIM
                     DTLINYHSLDIQWGNHDVLWVGAYAGSKVCLANLLRICARYDNLDIIEDAYGINLRPL
                     LTLAEKYYDADNPAFKPKKRPDKHERLTQREESQITKIHQAIAMIQFKLEIPIIKRRP
                     NFEMEERLVLEKVNYDTNEITVYGNTYPLKDTCFQTVNRDNPAELLPEEEEVMNKLLL
                     SFQQSEKLRRHMSFLMRKGSLYLPYNGNLLIHGCIPVDENGEMESFEIDGHTYSGQEL
                     LDVFEYHVRKSFDEKENTDDLSTDLVWYLWTGKYSSLFGKRAMTTFERYFIADKASHK
                     EEKNPYYHLREDVNMVRKMLSDFGLNADEGRIINGHTPVKEINGEDPIKADGKMLVID
                     GGFSKAYQSTTGIAGYTLLYNSFGMQLVAHQQFNAKEKILSEGIDELSIKRVVDKELQ
                     RKKIRDTNIGKDLQAQIDILKMLMHDRYLD"
     gene            7434..8513
                     /locus_tag="POB55_001183"
     CDS             7434..8513
                     /locus_tag="POB55_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500155.1"
                     /translation="MRILKESIIVAFAFVGVVVGAGFATGQEIFQFFTSHGAYSISGI
                     IVTGLLITLGGMVVMHTGHHLKSRNHSDSINYFLYPSIARGFDIILTMFMLSLAIIMT
                     AGGASTIHQSFNLPYWLSALILVAFILATLFLKFDRLIAVLGGVTPFLIAIVIMIAVY
                     YFTTSHLDFTAANNDAQIHKQKSLSPGWWFDAINYASLQIAAAFSFLSVMGSKVKYRD
                     STLYGGLIGGLIITFLLMMINLGLISQFDKIKHVDLPTLKLATQMSPSIGIIMSVIMI
                     LVIYNTVVGLMYAFASRFSVPFSRRYFIIIITMAVITYISTFIGFISLIGKVFPIMGL
                     FGFILLIPVLYKGLIKRITGKSHID"
     gene            complement(8618..9211)
                     /locus_tag="POB55_001184"
     CDS             complement(8618..9211)
                     /locus_tag="POB55_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDI6500156.1"
                     /translation="MEHIFREGQKGAPTLILLHGTGGDEFDLLPLGEALNENYHLLSI
                     RGQVSENGMNRYFKRLGEGVYDEEDLEFRGQELLAFIKEAAERYEFDIEKAVLVGFSN
                     GSNIAINLMLRSEAPFKKALLYAPLYPVEVTSTKDLSDVSVLLSMGKHDPIVPLAASE
                     QVINLFKTRGAVVEEVWVNGHEITETGLTAGQQILGK"
     gene            complement(9242..10201)
                     /locus_tag="POB55_001185"
     CDS             complement(9242..10201)
                     /locus_tag="POB55_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001628197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HDI6500157.1"
                     /translation="MANNHELLGIHHVTAMTDDAERNYKFFTEVLGMRLVKKTVNQDD
                     IYTYHTFFADDVGSAGTDMTFFDFPNITKGQAGTNSITRPSFRVPNDDALMYYEQRFD
                     EFGVKHEGIQELFGKKVLPFEEVDGQVYQLISDELNEGVAPGVPWKNGPVPVDKAIYG
                     LGPIEIKVSYFDDFKNILETVYGMTTIAHEDNVALLEVGEGGNGGQVILIKDDKGPAA
                     RQGYGEVHHVSFRVKDHDAIEAWATKYKEVGINNSGIVNRFYFEALYARVGHILIEIS
                     TDGPGFMEDEPYETLGEGLSLPPFLENKREYIESEIRPFNTKR"
     gene            complement(10278..10712)
                     /locus_tag="POB55_001186"
     CDS             complement(10278..10712)
                     /locus_tag="POB55_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDI6500158.1"
                     /translation="MDRTKQSLNVFVGMNRALDTLEQITKEDVKRYGLNITEFAVLEL
                     LYNKGPQPIQRIRDRVLIASSSISYVVSQLEDKGWITREKDKDDKRVYMACLTEKGQS
                     QMADIFPKHAETLTKAFDVLTKDELTILQQAFKKLSAQSTEV"
     gene            10938..11222
                     /locus_tag="POB55_001187"
     CDS             10938..11222
                     /locus_tag="POB55_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDI6500159.1"
                     /translation="MSNLEIKQGENKFYIGDDENHALAEITYRFVDNNEINIDHTGVS
                     DELGGQGVGKKLVKAVVEHARENHLKIIASCSFAKHMLEKEDSYQDVYLG"
     gene            11396..12201
                     /locus_tag="POB55_001188"
                     /pseudo
     CDS             11396..12201
                     /locus_tag="POB55_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(12322..12993)
                     /locus_tag="POB55_001189"
     CDS             complement(12322..12993)
                     /locus_tag="POB55_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDI6500160.1"
                     /translation="MSNMNQTIMDAFHFRHATKQFDPQKKVSKEDFETILESGRLSPS
                     SLGLEPWKFVVIQDQALRDELKAHSWGAAKQLDTASHFVLIFARKNVTSRSPYVQHML
                     RDIKKYEAQTIPAVEQKFDAFQADFHISDNDQALYDWSSKQTYIALGNMMTTAALLGI
                     DSCPMEGFSLDTVTDILANKGILDTEQFGLSVMVAFGYRQQDPPKNKTRQAYEDVIEW
                     VGPKE"
     gene            13263..14255
                     /locus_tag="POB55_001190"
     CDS             13263..14255
                     /locus_tag="POB55_001190"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HDI6500161.1"
                     /translation="MTKIMFFGTRDYEKEMALNWGKKNNVEVTTSKELLSSATVDQLK
                     DYDGVTTMQFGKLEDDVYPKLESYGIKQIAQRTAGFDMYDLDLAKKHNIVISNVPSYS
                     PETIAEYSVSIALQLVRRFPDIERRVQAHDFTWQAEIMSKPVKNMTVAIIGTGRIGAA
                     TAKIYAGFGATITAYDAYPNKDLDFLTYKDSVKEAIKDADIISLHVPANKESYHLFDK
                     AMFDHVKKGAILVNAARGAVINTPDLIAAVNDGTLLGAAIDTYENEAAYFTNDWTNKD
                     IDDKTLLELIEHERILVTPHIAFFSDEAVQNLVEGGLNAALSVINTSTCETRLN"
     gene            complement(14502..15302)
                     /locus_tag="POB55_001191"
     CDS             complement(14502..15302)
                     /locus_tag="POB55_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDI6500162.1"
                     /translation="MVKAIAVDMDGTFLDSKKTYDKSRFEAIFTELRNRDITFIAASG
                     NQYAKLKSIFGDRDMYFISENGAVIYNGNELYNYKSFNRQVFQQVVDYLNMNQSIDQL
                     VICGLKSAYILKHTSEAFKEDTRFYYHQLKEIDSLQQLPEDDYVKIAFNINRETHPNV
                     DEEVATQFSNDIKLVSSGHDSIDIIMPNMTKGQALKRLLDKWGMSSSELMAFGDANND
                     KDMLAFAKHSYVMENSHDEELFKIASAVAPSNDKQGVLTIIEQEVLNK"
BASE COUNT         5072 a         2772 c         2360 g         5285 t
ORIGIN      
        1 ggcagtaagc agtacaaaat gattacacaa aaatttcctt ctactcgata aattaagaag
       61 tataaaataa ttaaaaaaat acttataatc agacctataa gaggcaaact tctaatatat
      121 ttctctaagt tttccttcat atgtataacc tcctaaaatt tatatgtact tttacaagcg
      181 ttacctgact aatgttttaa aactttattt ctttacatat atcattttaa taacttcccg
      241 ttcatcttaa accatatctg ttttaaaaag tttcaatttc aacgtaaaaa agcaataagc
      301 cccaacaatg tgaaagctta ctgcttgttt aaattatttt tctattgctt tatcatcact
      361 aacaattaaa ggttgatctg gtttcacata gaaccacata atcacagcga ttactgctgg
      421 aacgattaac aattggaatg tcattgtcca acctaaatgt tctacgaata gacctgctaa
      481 tagcggactg attaatgcac ctatattacc ccataagttc atccaccctg atactgtacc
      541 agagaagtta cgtcctaagt cagttgctgc agcccaactc atacccattg aaataccgat
      601 accaccaaga cctagtgata accagaaaat acttacatat aaatttcctg tacgtacagc
      661 aaagataatt gaaactgcaa acacaataaa tcctgcgatt gcaattacac ctctagctac
      721 gaattttgaa cgacctaatc ctaatacacg gtcagaaatt gcacctgctg ataagattaa
      781 gaagaacatt aataaccaag gtaatgaact aatgctcatt tctttaaagt taacgtggaa
      841 tacttctgtt aaatacgtcg gtaaccaaat taagaataat gtaatgataa attgtacaac
      901 aaagtattga attgcaattg catagaagct aaaacgttta aagaaatcat tccatggtgg
      961 tgatgacttt tcagtagcta cgatatcacg attttccata atgaaacgtt tctccgcttc
     1021 attaaccatt ctatgttgct caggtaagtc tttggcaata atcgcccata atacagccat
     1081 taaaatacct actgcaccaa aaatgtaaaa tactgcttgc cagttaaatg cgttaacaat
     1141 agcaattgta actattggtg ctaatacagg tccgaaatat gatcctgcta ataatgcact
     1201 tgatgctcta cctttttcat ttttagagaa ccaaaatgag ttaaacacag cattagaagg
     1261 gtacattggc gcctcaccta caccaaataa gaatctcact aaataaatta aaccgtggtt
     1321 cttaatcata cccgttaaga ttgtaaatgc actccaccaa actaatgcaa ttgtaatcat
     1381 cttacgtgga ccgaattttt cagccaacat ccccgatggt acttgcatta atgcataacc
     1441 tagagagaag aatgatgcca ataagccaaa ttgtggtttg gtcatcccta aatcatccat
     1501 catttgttta gcgatatatg aaatgtttgc tctatccata tacgcaataa ccccaataaa
     1561 gaagaacgca agcgcgaaca tccaccttac gttagttcgt ttttccttca taactattcc
     1621 tacttccctt attaattttt aaaaacttac cctagaacga taaaactttc ggacatctgg
     1681 catgtccacc cctactttgt cacaaaattg taccaaagtt attttttata atattatcag
     1741 acatggaaag cgtttacaat agaatctata aaaattttat taaattccgc ttaacttttg
     1801 ctattaaaac gatataaagg aacttttagt tacatagtag cctatcattt gttaattaaa
     1861 aatactgcaa tcctagtgtt ttaaaacgta tattgcaggc tttcttacat attagacatt
     1921 cattttaaat tgcataaaac tacattgctc attcattgtg cagcgcctgt tatttagatg
     1981 atcatttgtt gacttttcgt acttatagca agcaattttt tgatttattg cttataatac
     2041 attttaagac aaatgcttgt ttcaacatcg catacgacat cattcattat tcatttgaat
     2101 ttatccagtt tctatttgtc tgcaattgct gaaaattaaa ggagcttatt tttaataatg
     2161 gaacaaatta tcactgaatt tattagtcgt tttgggtatg cagccatttt tatattaatt
     2221 ttattagaaa atgttttacc tatcgtacca tcggaaatta ttctgacatt tgctggcctt
     2281 atgtctgtta aatcacattt atcaatttta accttattta ttattgcaac catcgcatcg
     2341 tttatagggc tgttaatttt atattatatc tgtcgtttaa tttcagaaga acgtctatat
     2401 cgctttattg atcgacacgg taagtggatt aaattgaaaa gtaaggattt aaagcgagca
     2461 aatgattggt ttaaaaagta tggcgtatgg gctgtattta tctgtcgttt catacctgta
     2521 ttacgtgtat tgattaccat tccagccggt gtaaaccgca tgaatgttgt gacatttacc
     2581 gttatttcat taataggtac aacaatttgg aatttcggtt taattttact aggacgcacg
     2641 ttgagcgata gctttggtat gttgatgact ggtcttcata catattcacg tattatgtat
     2701 gttgtcatca ttatcgcagt catttacttt gctatacgtt acattggcaa acgtaagaga
     2761 gtaaaataat attgttgtta gtataatggt atattttcaa ttgctataaa taaaaagcag
     2821 taaaatcatt tcgatggctg gaatgattta ctgctttttt aggtttattt ggcatacttt
     2881 agttgaaagt catctaaaac cctttacgtt aagagatata atgaagtttc ccataaaagt
     2941 tcagttccct cagtatgtac catttattcc gcaaacgcat ctcacaatcc atctataata
     3001 caaactatca tcttaaataa gcagaattta ccctgtcttc cacacaaact gttttcgtca
     3061 ctttaatatg ccttctattc aaaaaagtca ctcataacaa attgcgcatt ttaataaatg
     3121 tagcaatgcc atttgcatgc tacaaaaata ctcgtattca tatttatctt ttatattatt
     3181 aaagtttttt aaatccgtat ttttataaca cttgctatgt gataaaatta attttatata
     3241 taaaatctta agattcagat tatttaatag caaaggagat agtgatatgg atgttttaac
     3301 aatagaacat ttaacaaaga agataggcaa caaaacgatt ctcgaagatg tatcatttaa
     3361 gctgaaacgc ggacaaatag ttggtctcgt tggagcgaat ggtgcaggta aaacaacttt
     3421 aatgaaagtt atattaggtt actctagttt ccaaagcggg aattttaatg ttattaacag
     3481 caaagacagc aaaagcaata tcggtgcatt gattgaaaat ccaggaatat atccttttat
     3541 gtctggatat gaaaacttga agttattgaa tgaatcaaaa aacactcaag atatcgataa
     3601 aattgtctca caacttcata tggatgaata cattcataaa aaagctaaaa cgtattctct
     3661 tggtatgaaa caaaaattag gaattgctat agcattttta aatgaacctc aatttattat
     3721 cttagatgaa ccaatgaatg gcttagatcc aaaagctgtg cgagatgttc gtgaattgat
     3781 tgtccaaaaa gcgcaagaag gtgttacttt cttaatttcg agtcatattt taagtgaatt
     3841 agttaaaatc acaaactcta tccttattat taacaaaggt aaaattgtta cagaaacatc
     3901 ggaagaagaa cttaaacaat ttaaagataa tgatttagaa aatgtattac tagaaatcat
     3961 agaaagggag gaccaagcat aaaatgggaa ctttaattaa acaagaatgt ttcaaattat
     4021 ttaaaaagaa atcaactttt atcgcaccta ttgtctttat tctactaatg gttgctcaag
     4081 gttatattgc tacaaaatac aataaaattt ttacgccaca ggaatctttc acatctgctt
     4141 ataatggttt ttcatggttt gcatttttat taattattca agcaagtaca atcatttcaa
     4201 tggaatttca ttacggtacg attaaaaatt tactctatcg tgaatattca agaacaacta
     4261 tgattgttag caaaatcatc acattattta ttatttcttt aatttatttt gttattacaa
     4321 ttattgcttc aattgttatt gggtctttat tctttaatga tttaaatata tttgaaagta
     4381 gcggtaatca attatcttta ttgaatcaat tattattagt tagtttaggc acatttgttg
     4441 gcgtttggtt agttttaagc ttaacgttgc tattatcatc tgcaacaaat tcaacgggag
     4501 tagccattgc tgtaggtatt gttttttatt ttgcaagttc tattttagca gttattcaaa
     4561 cggcactttt agaaaaaata gactggctaa agtggaatcc tattaatatg atgaatatta
     4621 tgcttcaaac agttgaaaaa ggctttagta agtcgacgaa attagaactt catgaattgt
     4681 ttattggtaa tattgcttat atttctattt tcttaatact tgtagtattt attttcaaga
     4741 agaaaaatat ttagtaactt caagttatta aatgtctaaa tacacacata ttccatcgta
     4801 attcaaaatc attttcaaat cccttcaccc aaataatggt gcggggattt ttttatccaa
     4861 attttggcaa ttccacattt tacatatcgt aattttttag gaaactagtg aatataacaa
     4921 attcctcctc tcatttttaa aataaatata tcactttccc acttttactt aactaaactg
     4981 caacgactcc taataccaaa atcctgccct ctatttttat caatccaagc atacttattg
     5041 aaaaatgtta acgttttctt gataatcatt gtaagcgcat ttattttata aactaacgtt
     5101 tgaaatatac tacaggagtg acacgtaatg actcaaatta ctgaaaaaga attaaaaaag
     5161 aagtatttag atttactatc ccaaaatttt gatactccag aaaaacttgc aactgaaatt
     5221 atcaatttag aatcaatttt agaattacct aaaggtacgg aacatttcgt cagcgattta
     5281 catggtgaat acgaagcttt ccaacacgta ttacgcaacg gttctgggaa tgtgcgagcg
     5341 aaaatcaatg atattttcaa agagagactt tcaactaagg agcttaatga tttaactgct
     5401 cttgtctact atccagaaga caaattaaaa ttgattaaaa gtgatttcca aagttgcggt
     5461 caacttaatg tctggtatat cacaacaatc gaacatttaa ttgagttgat taaatattgt
     5521 tcttcaaaat atacgcgttc aaaactgcgt aaagcattgc caaaacaata cgtttatatt
     5581 attgaggaac tactgtataa aagtaatgaa tatcaaaata aaaaatcata ttacgaaaca
     5641 cttgttaacc aagtaattga acttaaacag gcagatgatt taattattgg actcgcttat
     5701 tccgtacaac gcttagtcgt cgatcattta cacgttgtcg gtgatattta tgatcgtgga
     5761 ccacaaccag ataaaattat ggatacactg attaattatc attccctaga tattcaatgg
     5821 ggtaatcatg atgtgctttg ggttggagcc tatgctgggt caaaagtatg cttagcaaac
     5881 ttacttcgaa tttgtgcacg ctatgataat ttagatatta tcgaagacgc ttatggcatt
     5941 aatttaagac cactgcttac tttagctgaa aaatactatg acgcagataa tcctgctttt
     6001 aagcctaaaa aaagacctga caaacacgaa cgtttaactc aacgtgaaga aagtcaaatt
     6061 actaaaattc atcaagctat tgcgatgatt caattcaagt tagaaatacc aattattaaa
     6121 cgtcgtccaa atttcgaaat ggaagaacgt cttgtgcttg aaaaggttaa ttatgataca
     6181 aatgaaatta cagtttatgg taatacatac ccattgaaag acacatgttt ccaaactgtc
     6241 aatcgtgata atccagcaga attactacct gaagaagaag aagtcatgaa taaactatta
     6301 ttgtcattcc aacaatctga aaaattacgt cgtcatatgt ctttcttgat gcgtaaaggc
     6361 tctctttact taccatataa tggcaattta ctcattcatg gttgtattcc agttgatgaa
     6421 aatggtgaga tggaatcatt tgaaattgat ggtcatactt acagcggcca agaattatta
     6481 gatgtatttg agtatcatgt ccgtaaatca tttgatgaaa aagaaaatac tgatgactta
     6541 tcgacggatt tagtttggta tttatggact gggaaatatt cgtcattatt tggaaaacgt
     6601 gccatgacta cgtttgaacg atactttatt gcagataaag cttctcataa agaagaaaaa
     6661 aatccatact atcatctacg tgaagatgtg aatatggttc gtaaaatgct cagtgatttt
     6721 ggattaaatg cagatgaagg tcgcattatt aatggtcaca caccagtgaa agaaatcaat
     6781 ggcgaagatc ctatcaaggc tgatggaaag atgcttgtca ttgatggtgg cttttcaaaa
     6841 gcttatcagt caacaactgg cattgcaggc tatacactat tgtataattc attcggtatg
     6901 caactcgttg cacaccaaca atttaacgct aaagaaaaaa tactttccga aggtatcgat
     6961 gaactttcta taaagcgcgt cgtagataaa gaattacaac gtaagaagat tcgtgatacc
     7021 aatattggta aagatcttca agctcaaata gatattttga aaatgttaat gcatgatcgc
     7081 tacttagact aaagacgaca ttttatgcat gtattgtata ctgttgtatt gatgcggttt
     7141 acgcattggc tacgcaaacg cgatgataaa taggcgacct agctacaccg aaaaaaatcc
     7201 cctcactatt gcgagtagtg aggggattgg tgtatctatg ctaattcctg ttaactgtat
     7261 tataacaact ttacagcaaa aaaatgcaaa atcaactaat aaattaatga aatgaccgac
     7321 gccctcgctt ctacttcaat ttaacaaaat actatattag tactatattt ttcattaaca
     7381 tgtatgttac attttctata ttacttaaaa ttacgaaggg acatttttaa atcatgcgta
     7441 tcttaaaaga gtccattatt gtggcatttg cctttgttgg tgttgtcgtt ggtgccggct
     7501 ttgctactgg tcaagaaatt ttccagtttt tcacaagtca tggcgcatat agcatttcag
     7561 gcattattgt aacaggacta ttgattactt taggtggaat ggttgtcatg catacaggtc
     7621 atcatctaaa gtccagaaat cattctgatt caattaacta tttcttatac ccctctattg
     7681 caagaggttt tgatattatt ttgacaatgt ttatgttgtc tttagctatt attatgactg
     7741 caggtggtgc gtcaaccatt catcaaagtt tcaacttacc gtattggctg agcgcactca
     7801 tattagtcgc ctttatttta gcaacactgt ttctaaaatt cgatcgttta attgctgtgc
     7861 ttggcggtgt taccccattt ttaattgcga ttgtcattat gattgcggtc tactatttca
     7921 caacaagtca tcttgatttt actgccgcta ataatgatgc tcagattcat aagcagaaat
     7981 cattatcacc tggatggtgg tttgatgcga ttaactatgc aagcttgcaa attgctgctg
     8041 ccttcagctt cttatcagtg atgggtagta aagttaaata tcgtgactca acgttatacg
     8101 ggggcttgat tggcggttta atcattacat ttttactcat gatgattaac ctaggtttaa
     8161 tttctcaatt cgataaaatt aaacacgtcg atctacctac attaaaatta gcgacacaaa
     8221 tgtctccgtc aattggtatt attatgtctg tcattatgat acttgtcatc tacaatactg
     8281 ttgttggatt aatgtatgca tttgcgtcac gtttcagcgt tccatttagc agacgttact
     8341 tcatcattat tattacaatg gctgtcatca cttatattag tacatttatc ggtttcattt
     8401 cattaattgg aaaagtattc cctattatgg gattgttcgg tttcatctta ctcatacctg
     8461 tactctataa aggtttaatt aagcgtatta ccggcaaatc tcatatcgat taaaatatct
     8521 atactattgt aaaatttagt acaacacttc aaaatcaaaa aagcgggcag atgaaaagta
     8581 aaaatctttt ccatctgtcc gcttcttaat agaactgtta tttcccaagt atttgttgac
     8641 cagccgttaa tccagtttct gtaatttcat gaccattcac ccaaacttct tcgactactg
     8701 ccccacgtgt cttaaacaag ttaatgactt gttcacttgc agctaatggc acaattggat
     8761 catgtttccc catagaaagc aacacactta catctgataa atcctttgtt gacgttactt
     8821 caactgggta taacggtgca tataacaatg cttttttaaa tggtgcttcc gaacgcaaca
     8881 ttaagttaat cgctatattt gatccatttg aaaatccaac aagtactgct ttttcaatat
     8941 caaattcata acgttcagca gcttctttaa tgaacgctaa caattcttgt ccacgaaatt
     9001 ccaaatcttc ttcatcataa acaccttcac caagacgttt gaaataccgg ttcatcccat
     9061 tttctgaaac ttgtcctcta atacttaaca agtgataatt ttcattcaat gcttcgccta
     9121 atggcaataa atcgaattca tcgccacctg taccatgcaa caatattagc gttggcgcac
     9181 ctttttgtcc ttcacgaaaa atatgttcca tcacaaatcc tcctcattcc aattaatcat
     9241 gttaacgctt agtattaaat ggtcttattt ctgattctat atactctctt ttattttcta
     9301 aaaatggtgg caatgataaa ccttcaccta atgtttcata cggttcatct tccataaatc
     9361 ctggtccatc tgttgaaatt tctattaaaa tatgccccac acgtgcatat aatgcttcaa
     9421 aatagaaacg attaacgatg cctgagttgt taatacctac ctctttatat ttcgttgccc
     9481 acgcttctat tgcatcatga tctttcacac gaaatgacac atgatgtacc tcaccataac
     9541 cttgacgtgc tgctggccct ttatcatctt ttattaagat tacctggcca ccattgcctc
     9601 cttcgccaac ttcaagtaat gcgacattat cttcatgcgc aatagttgtc ataccgtaaa
     9661 cagtctctaa aatattttta aagtcgtcaa aataacttac tttaatttca atgggtccta
     9721 atccataaat cgctttatct actggaaccg gtccattctt ccaaggtaca ccaggtgcta
     9781 ccccttcatt taactcatct gaaattaatt gatacacttg gccatcgact tcttcaaatg
     9841 gcaacacttt tttaccaaat aattcttgaa taccttcgtg tttaacacca aattcatcaa
     9901 agcgctgttc ataatacatt aatgcgtcat cgttaggcac tctaaaagac ggtcttgtaa
     9961 tggaatttgt tcctgcctgc ccttttgtaa tatttggaaa atcaaagaac gtcatgtctg
    10021 tacctgccga acctacatca tctgcaaaaa aagtatgata cgtataaata tcatcttgat
    10081 tgactgtctt tttaactaaa cgcatgccta gtacttctgt aaaaaattta taattacgtt
    10141 ctgcatcatc tgtcattgct gtaacatggt gaatacctaa tagttcatga ttatttgcca
    10201 ttttatatac cctctttgtc ttttagcata tttatctcga aaccgagata ttttaatgta
    10261 aatttttagt gcacgcctta cacttctgta gattgtgcac ttaatttctt aaacgcttgt
    10321 tgtaagattg ttaattcatc ctttgttaac acatcaaacg cttttgttaa tgtctcagca
    10381 tgcttaggga aaatatctgc catttgactt tgaccttttt cagttaaaca agccatatat
    10441 acacgtttat catctttatc cttttcacgt gtaatccaac ctttgtcctc taattgactt
    10501 acaacatatg aaatgctgct acttgcaatt aatacgcggt ctctaatacg ttgaattggt
    10561 tgcggacctt tattataaag caactcgagc actgcaaatt cagtaatatt taagccatat
    10621 cgctttacgt cttcttttgt aatttgctct aatgtgtcta acgccctatt cattccgaca
    10681 aagacattga gcgattgttt cgttcgatcc atatcatcac caacttttta atttatttcg
    10741 atttcgagat aaattatata gctaaatgaa tttaatgtca agatacgtgt tttcatcttc
    10801 atttcaaact gcgcttaata aaaacgacta cataaatgtg gcaatgtcgt cacatgactg
    10861 tcatgcacct ttgacttcgt ttaagttaaa atagaatagg gaaatactat aatcaatcat
    10921 gataggagtt gatatgtatg agtaaccttg aaattaaaca aggcgagaac aaattctata
    10981 ttggtgatga tgaaaatcat gctttagctg aaatcacata ccgttttgtg gataataatg
    11041 aaattaacat tgatcataca ggcgtatctg atgaactcgg tggtcaaggt gttggcaaaa
    11101 aactagttaa agcagttgtt gaacatgctc gcgaaaatca tttaaaaatc attgcctcgt
    11161 gttcatttgc caaacatatg ttagaaaaag aagattcata tcaagatgta tatcttggtt
    11221 aaataaattt tgcgatggtg caactgtgcc atcgcttttt atttgtacga atcaatataa
    11281 aattgcaaaa cgacaagtta ttttgcattt caaactttat gctatcaatt cattttcttt
    11341 tctggtatac tttttatact aacttataga aaaatagaaa acgaggcgat gcataatggc
    11401 atttcacgat aaaacagcaa cacaagtgac aaacattgaa ctgaacgtaa gagacttaga
    11461 cttaatgaca acattttaca aaaacatttt aggactatcc gttaaaagtt ctgacgataa
    11521 cacaaccgta ttatctgttg ggactggtgg ccatactcta acgttacatt tattagaaga
    11581 cggccgtcag acttccccac gtgaagcagg gctttttcat atagcacttt tattaccaac
    11641 tactgaggat ctagctaact tcttatattt cgtggcacaa aaaacatggg catcggcgct
    11701 ggtgatcatt tagtaagtga agctttatat ttcaacgacc ccgaaggtaa tggtattgaa
    11761 gtctatcgcg atagaccttc atcttcatgg gaatggcaaa atggcaaagt aaaaatggat
    11821 actttagaag ttgatagcca aactttatta acacatcgta ctgacgaagg ttggcaagga
    11881 atgccagcaa aaggcatgat tggacactta catttaaaaa cacatgattt ggacgcagca
    11941 tatcaatgtt acattgaaca attaggattc caacatgtgt ctgattttcc acgtgcgtta
    12001 tttatgtcaa cgaatcatta tcaccatcat attgcggcta acacttggca atccaatcaa
    12061 gcaagacaaa acaatgcaca aagttatgga ttaacacatg ttgatattta tcaacccgac
    12121 gcctcaacac atacatttac ggcaccagaa gggtttgaca tcacagttca ttcaaatact
    12181 gaccttgttc cagaaaaata atcatccata ctttattaat acaatttttt cacgcaaaag
    12241 cccgcgattc atttatatga acgcaggctt ttattattgc taacttttta attatatata
    12301 tttagacatg ctgtattcta tttattcttt tggtccaacc cattcaataa catcttcata
    12361 tgcttggcgt gttttatttt tcggtggatc ttgttgtctg tagccaaatg caaccatcac
    12421 tgataaacca aattgctcag tatctaagat acctttattt gctaaaatgt ctgtcactgt
    12481 atccagacta aaaccttcca tcggacatga atcaatacct aacaatgcgg ctgtcgtcat
    12541 catattgcct aatgcgatat acgtttgttt acttgaccag tcatacaagg cttgatcatt
    12601 atcagaaata tggaaatctg cttggaatgc atcgaatttt tgttcaacag ctgggatcgt
    12661 ttgtgcctca tattttttaa tatctcttaa catatgttgt acatacggtg atcttgacgt
    12721 tacattttta cgcgcaaaaa ttagcacaaa atggctcgct gtatctaatt gttttgctgc
    12781 gccccaactg tgcgctttta attcatcacg taacgcttga tcttgaatca cgacaaactt
    12841 ccaaggttct aacccaagag aacttggaga caatctacct gactctaata ttgtttcaaa
    12901 atcttctttc gaaactttct tttgtggatc gaattgctta gtcgcatgtc tgaaatgaaa
    12961 tgcatccata attgtttgat tcatattgct cattgaacgt agcctccaat ttttatattt
    13021 attactatac aaattatact tcatgtttta aaaagactaa agtaatccgt ttgcaattca
    13081 ctataacttg tgcacatgtt ataagttttc tgcaataaaa cacacttttg aattttggaa
    13141 aatttagtgt tataatatat ttgtgatatt tttcacaatt aaattgtggc gtgattttaa
    13201 tgtacataat ctttaatttc actcatttac ttttcaatct tttaaaagcg aggtttttaa
    13261 taatgacaaa aattatgttc tttggtacgc gtgattatga gaaagagatg gcattaaatt
    13321 gggggaaaaa gaataatgtc gaagtaacta cttctaaaga gctattatca agtgctacag
    13381 tcgatcaatt aaaagattac gatggcgtaa ctacaatgca atttggtaag ttagaagatg
    13441 acgtttatcc taaattagaa tcttacggta ttaaacaaat tgcacaacgt actgctggat
    13501 ttgatatgta tgatttagat ttagctaaaa aacacaacat tgtgatatct aacgttccta
    13561 gttattcacc tgaaacaatt gcagagtatt ctgtttctat cgccctacaa ttagtgcgtc
    13621 gcttcccaga tattgaacgc cgtgtacaag cacatgattt tacttggcaa gcagaaatca
    13681 tgtctaaacc agttaaaaat atgactgttg caattatcgg tacgggtcgt atcggtgctg
    13741 ctactgctaa aatatatgca ggatttggtg ctacaattac agcttatgac gcttatccta
    13801 ataaagattt agacttttta acttataaag atagtgttaa agaagctatt aaagatgccg
    13861 atattatttc tttacatgtt ccagcgaaca aggaaagcta tcatttattc gataaagcaa
    13921 tgtttgatca tgtgaaaaaa ggtgcaattt tagttaacgc agcacgtggt gcagtcatca
    13981 atacacctga tttaatcgct gcagtgaacg atggtacttt gttaggtgct gcgattgata
    14041 cttatgaaaa tgaagcagca tacttcacaa atgactggac taataaagac attgacgata
    14101 aaacattatt agagttaatc gaacatgaaa gaattttagt aacaccacat attgctttct
    14161 tctctgatga agcagtacaa aaccttgttg aaggtggttt aaacgcagca ttatctgtca
    14221 ttaacactag tacatgtgaa acacgtttaa attaatatat atttaaaagc aaaggcgcgc
    14281 catgtgcctt tgctttttat tttgtatatc tgtataaaaa ccagtttgat taaaatccgc
    14341 tttattttat catctattca aatgatttta gtgcgcttat tttactatgg cattattgcc
    14401 taacgtgttt gagatatatt aatatttgat tattcgtatt caaagttttc atttaacatg
    14461 tatttagtat cataatagcc gcttcattga tgatgtacta cctacttgtt caatacttct
    14521 tgttcgataa tcgttaaaac accctgctta tcattacttg gagcgacagc agaggcaatc
    14581 ttgaacaatt cttcatcatg actattttcc atcacataac tatgcttagc gaacgcaagc
    14641 atatctttat cattattcgc atctccgaag gccatgagct ctgaagaaga cattccccat
    14701 ttatctaaca atcgttttaa tgcctgcccc ttagtcatgt ttggcataat aatatcaatg
    14761 ctatcgtgcc cacttgagac aagtttaata tcattgctga attgcgttgc tacttcttcg
    14821 tcaacatttg gatgcgtctc acgattaata ttaaatgcta ttttgacata atcatcctca
    14881 ggtaattgtt gtagactgtc aatttctttt aactgatgat aataaaatct cgtatcttct
    14941 ttaaatgctt cagaagtatg ttttaaaata tacgcgcttt tcaaaccaca gatgacgagt
    15001 tgatcaatac tttggttcat atttaaataa tcgacaacct gttgaaacac ctgacgatta
    15061 aagcttttat aattatataa ctcattgcca ttataaataa ctgcaccatt ttcagaaata
    15121 aaatacatat ctctatcccc gaaaatagac ttcaacttcg catattgatt gccactcgca
    15181 gcaataaatg taatatctct atttctaagt tcagtaaaaa tcgcttcaaa tcttgattta
    15241 tcgtatgtct ttttcgaatc taaaaacgtc ccgtccatat ctaccgcaat agccttaacc
    15301 atgattatcc tcctcattgc tcttttagtc gtcttcatta tatccatctc atgacaagca
    15361 atgaagtttg tcataccaca atgaacctat attcacattt tgaaattgct ttcatatatg
    15421 caaaacacat tacactggat atagcattca gaggcgaaca taaatttcag aatattgtaa
    15481 aaatataac
//
LOCUS       DAMZKC010000050        15314 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.050, whole genome shotgun sequence.
ACCESSION   DAMZKC010000050 DAMZKC010000000
VERSION     DAMZKC010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 15314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15314
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.050"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            21..1004
                     /locus_tag="POB55_001192"
     CDS             21..1004
                     /locus_tag="POB55_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDI6500163.1"
                     /translation="MDKETNDNEYRRQSEHRTSAPKRKKKKKIRKLPIILLIVVILLI
                     ALVVYIVHSYNSGVEYAKKHAKDVKVHQFNGPVKNDGKISILVLGADKAQGGQSRTDS
                     IMVVQYDFINKKMKMMSVMRDIYADIPGYGKHKINSAYALGGPELLRKTLDKNLGINP
                     EYYAVVDFTGFEKMIDELMPEGVPINVEKDMSKNIGVSLKKGNHRLNGKELLGYARFR
                     HDPEGDFGRVRRQQQVMQTLKKEMVNFRTVVKLPKVAGILRGYVNTNIPDSGIFQTGL
                     SFGIRGEKDVKSLTVPIKNSYEDVNTNTDGSALQINKNTNKQAIKDFLDED"
     gene            complement(1092..1277)
                     /locus_tag="POB55_001193"
     CDS             complement(1092..1277)
                     /locus_tag="POB55_001193"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HDI6500164.1"
                     /translation="MPIVNVKLLEGRSDEQLKNLVSEVTDAVEKTTGANRQAIHVVIE
                     EMKPNHYGVAGVRKSDQ"
     gene            1425..2687
                     /locus_tag="POB55_001194"
     CDS             1425..2687
                     /locus_tag="POB55_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HDI6500165.1"
                     /translation="MYNYHLLEDRDVLCIDQKSFFASVSCIEKGLDPLETKLAVVADT
                     KRQGSVILAATPKLKELGIKTGSRLFEIPHRNDIYIINPSMRKYLNVSVAISKIALRY
                     IPPEDLHQYSIDEFFMDVTDSYHRFSSTVHAFCERLKREIYEETGIYCTVGIGSNMLL
                     SKIAMDVEAKHSQNGIAEWRYQDVPTKLWPIQPLRDFWGINRRTEAKLNKRGIFTIGD
                     LAKYPYKFLKKEFGILGVDMHLHANGIDQSKVREKHKISNPSICKSQILMRDYHFDEA
                     KVVMQELIEDVASRVRARKKVARTIHFAFGYSDEGGVHKQYTLKDPTNLEKDIYKVVM
                     HFADKLCNKQALYRTLSISLSQFINEDERQLSLFEDEYQRKRDECLAKTIDQLHLKYG
                     KGIVSKAVSFTEAGTKHGRLGLMAGHKM"
     gene            complement(2823..3914)
                     /locus_tag="POB55_001195"
     CDS             complement(2823..3914)
                     /locus_tag="POB55_001195"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HDI6500166.1"
                     /translation="MTTVLFVGLGLIGGSLASNIKYHNPNTNIIAYDADTSQLDKAKS
                     IGIINEKCLNYSEAIKKADVIIYATPVAITNKYLSELIDMPTKPGVIVSDTGSTKAMI
                     QQHECNLLKHNIHLVSGHPMAGSHKSGVLNAKKHLFENAYYILVYNEPRNEQAANTLK
                     ELLSPTLAKFIVTTAEEHDYVTSVVSHLPHIVASSLVHVSQKNGQEHHLVNKLAAGGF
                     RDITRIASSNAQMWKDITLSNKTYILEMIRQLKSQFQDLERLIESNDSEKLLSFFAQA
                     KSYRDALPAKQLGGLNTAYDLYVDIPDESGMISKVTYILSLHNISISNLRILEVREDI
                     YGALKISFKNPTDRERGMQALSDFDCYIQ"
     gene            4079..5110
                     /locus_tag="POB55_001196"
     CDS             4079..5110
                     /locus_tag="POB55_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HDI6500167.1"
                     /translation="MEPILEMIKTLTGINSPSGDTEEAIQFVEKYAKDLGYQTTLTNK
                     GALLITVPGKNDEVQRCITAHVDTLGAMVKEIKEDGRLAIELIGGFTYNAIEGEYCQI
                     KTDAGQIYTGTICLHETSVHVYRNNHEIPRDQKHMEIRIDEVTTSEEDTKSLGISVGD
                     FVSFDPRTVITSSGFIKSRHLDDKASVAIILQLLKKLKEEQIILPHTTQFYISNNEEI
                     GYGANASIDSKIKEYIALDMGALGDGQASDEYTVSICAKDASGPYHKQLKSHLVNLCK
                     INNIPYKVDIYPYYGSDASAALHAGADIRHGLFGAGIESSHAMERTHIDSIKATEKLL
                     YAYCLSPIE"
     gene            5602..7008
                     /locus_tag="POB55_001197"
     CDS             5602..7008
                     /locus_tag="POB55_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HDI6500168.1"
                     /translation="MDIFYKKIKANVTPEVLAQLHSKKIILESTNQQQTKGRYSVVIF
                     DIYGTLTLDNDVLSVSTLKESYQISERPYHYLTTKINEDYHNIQDEQLKSLPFISGYV
                     GTCSFDLVRHEFPKLQSIQLEDHKQHDVRLYMVEQVYVFDHYKDELYIIATNQFSNST
                     KSDLENRVNKSIEDLTKIQPFMPTQDFDFKTKEIQSNISEERFIEMIQYFKEKITEGD
                     MFQVVPSRIYKYAHHASQHLNQLSFQLYQNLKRQNPSPYMYYLNIDQPYIVGSSPESF
                     VSVKDQIVTTNPIAGTIQRGETTQIDNENMKQLLNDPKECSEHRMLVDLGRNDIHRVS
                     KIGTSKITKLMVIEKYEHVMHIVSEVTGKINQNLSPMTVIANLLPTGTVSGAPKLRAI
                     ERIYEQYPHKRGVYSGGVGYINCNHNLDFALAIRTMMIDEQYINVEAGCGVVYDSIPE
                     KELNETKLKAKSLLEVSP"
     gene            7005..7571
                     /locus_tag="POB55_001198"
     CDS             7005..7571
                     /locus_tag="POB55_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HDI6500169.1"
                     /translation="MILVVDNYDSFTYNLVDIVAQHTDVIVQYPDDDNVLNQSVDAVI
                     ISPGPGHPLDDQQLMKIISTYQHKPILGICLGAQALTCYYGGEVIKGDKVMHGKVDTL
                     KVISHHQHLLYQDIPEQFSIMRYHSLISNPDNFPEELKITGRTKDCIQSFEHKERPHY
                     GIQYHPESFATDYGVKIITNFINLVKEG"
     gene            7577..8575
                     /gene="trpD"
                     /locus_tag="POB55_001199"
     CDS             7577..8575
                     /gene="trpD"
                     /locus_tag="POB55_001199"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HDI6500170.1"
                     /translation="MTLLTRIKTETILLESDIKELIDILISPSIGTDIKYELLSSYSE
                     REIQQQELTYIVRSLINTMYPHQPCYEGAMCVCGTGGDKSNSFNISTTVAFVVASAGV
                     KVIKHGNKSITSNSGSTDLLNQMNIQTTTVDDTPNQLNEKDLVFIGATESYPIMKYMQ
                     PVRKMIGKPTILNLVGPLINPYHLTYQMVGVFDPTKLKLVAKTIKDLGRKRAIVLHGA
                     NGMDEATLSGDNLIYELTEDGEIKNYTLNATDYGLKHAPNSDFKGGSPEENLAISLNI
                     LNGKDQSSRRDVVLLNAGLSLYVAEKVDTIAEGIELATTLIDNGEALKKYHQMRGE"
     gene            8577..9359
                     /gene="trpC"
                     /locus_tag="POB55_001200"
     CDS             8577..9359
                     /gene="trpC"
                     /locus_tag="POB55_001200"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HDI6500171.1"
                     /translation="MTILSEIVKYKQSLLQNGYYQDKLNTLKSVKIQNKKSFINAIEK
                     EPKLAIIAEIKSKSPTVNDLPERDLSQQISDYDQYGANAVSILTDEKYFGGSFERLQA
                     LTTKTTLPVLCKDFIIDPLQIDVAKQAGASMILLIVNILSDKQLKDLYNYAISQNLEV
                     LVEVHDRHELERAYKVNAKLIGVNNRDLKRFVTNVEHTNTILENKKTNHYYISESGIH
                     DASDVRKILHSGIDGLLIGEALMRCDNLSEFLPQLKMQKVKS"
     gene            9359..9991
                     /locus_tag="POB55_001201"
     CDS             9359..9991
                     /locus_tag="POB55_001201"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HDI6500172.1"
                     /translation="MKLKFCGFTSIKDVTAASQLPIDAIGFIHYEKSKRHQTITQIKK
                     LASAVPNHIDKVCVMVNPDLTTIEHVLSNTSINTIQLHGTESIDFIQEIKKKYSSIKI
                     TKALAADENIIQNINKYKGFVDLFIIDTPSVSYGGTGQTYDWTILKHIKDIPYLIAGG
                     INSENIQTVNQLKLSHQGYDLASGIEVNGRKDIEKMTAIVNIVKGDRDNE"
     gene            9984..11198
                     /gene="trpB"
                     /locus_tag="POB55_001202"
     CDS             9984..11198
                     /gene="trpB"
                     /locus_tag="POB55_001202"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HDI6500173.1"
                     /translation="MNKQIQTEADELGFFGEYGGQYVPETLMPAIIELKKAYKEAKAD
                     PEFQRELEYYLSEYVGRATPLTYAASYTESLGSAKIYLKREDLNHTGAHKINNALGQA
                     LLAKRMGKKKLVAETGAGQHGVASATVAALFDMELVVFMGSEDIKRQQLNVFRMELLG
                     AKVVAVEDGQGTLSDAVNKALQYWVSHVDDTHYLLGSALGPDPFPTIVRDFQRVIGKE
                     IKSQILKKEGRLPDAIVACIGGGSNAIGTFYPFIKDDVALYGVEAAGQGDDTDKHALA
                     IGKGSPGVLHGTKMYLIQDEDGQVQLAHSISAGLDYPGIGPEHSYYHDIGRVTFENAS
                     DTQAMNALINFTKHEGIIPAIESAHALSYVERLAPTMSKEDIIVVTISGRGDKDMETI
                     RQYMVERGLAND"
     gene            11191..11919
                     /locus_tag="POB55_001203"
     CDS             11191..11919
                     /locus_tag="POB55_001203"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HDI6500174.1"
                     /translation="MTKLFIPYIMGNKDLIENATLLSENGADIIEIGVPFSDPVADGP
                     VIMEAGQQAIKQGITIDYIFNQLEKHGDQIKCNYVLMTYYNIICHYGEQAFFEKCRDT
                     GVYGLIIPDLPYELSQRLKQQFSHYGVKIISLVAMTTDDKRIKDIVSHAEGFIYTVTM
                     NATTGQNGAFHPELKRKIESIKAIANVPVVAGFGIRTPQHVADIKEVADGIVIGSEIV
                     KRFKSNTREEIIKYLQSIQQTLNN"
     gene            12241..13503
                     /gene="femA"
                     /locus_tag="POB55_001204"
     CDS             12241..13503
                     /gene="femA"
                     /locus_tag="POB55_001204"
                     /EC_number="2.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine glycyltransferase FemA"
                     /protein_id="HDI6500175.1"
                     /translation="MKFTNLTAKEFGAFTDSMPYSHFTQTVGHYELKLAEGYETHLVG
                     IKNNNNEVIAACLLTAVPVMKVFKYFYSNRGPVIDYENQELVHFFFNELSKYVKKHRC
                     LYLHIDPYLPYQYLNHDGEITGNAGNDWFFDKMSNLGFEHTGFHKGFDPVLQIRYHSV
                     LDLKDKTADDIIKNMDGLRKRNTKKVKKNGVKVRFLSEEELPIFRSFMEDTSESKAFA
                     DRDDKFYYNRLKYYKDRVLVPLAYINFDEYIKELNEERDILNKDLNKALKDIEKRPEN
                     KKAHNKRDNLQQQLDANEQKIEEGKRLQEEHGNELPISAGFFFINPFEVVYYAGGTSN
                     AFRHFAGSYAVQWEMINYALNHGIDRYNFYGVSGKFTEDAEDAGVVKFKKGYNAEIIE
                     YVGDFIKPINKPVYAAYTALKKVKDRIF"
     gene            13522..14781
                     /gene="femB"
                     /locus_tag="POB55_001205"
     CDS             13522..14781
                     /gene="femB"
                     /locus_tag="POB55_001205"
                     /EC_number="2.3.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine glycyltransferase FemB"
                     /protein_id="HDI6500176.1"
                     /translation="MKFTELTVTEFDNFVQNPSLESHYFQVKENIVTRENDGFEVVLL
                     GIKDDNNKVIAASLFSKIPTMGSYVYYSNRGPVMDFSDLGLVDYYLKELDKYLQQHQC
                     LYVKLDPYWLYHLYDKDIVPFEGREKNDALVNLFKSHGYEHHGFTTEYDTSSQVRWMG
                     VLNLEGKTPETLKKTFDSQRKRNINKAINYGVKVRFLERDEFNLFLDLYRETEERAGF
                     VSKTDDYFYNFIDTYGDKVLVPLAYIDLDEYVLKLQQELNDKENRRDQMMAKENKSDK
                     QMKKIAELDKQIDHDQHELLNASELSKTDGPILNLASGVYFANAYEVNYFSGGSSEKY
                     NQFMGPYMMHWFMINYCFDNGYDRYNFYGLSGDFTENSEDYGVYRFKRGFNVQIEELI
                     GDFYKPIHKVKYWLFTTLDKLRKKLKK"
     gene            15070..>15314
                     /locus_tag="POB55_001206"
     CDS             15070..>15314
                     /locus_tag="POB55_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001558444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500177.1"
                     /translation="MNRIEKHAKNTFIILMLIMLFWIFMSFIFQKLLFPPSKNNLTTY
                     EALKYYTHLKGYYGLDHISKGIAYIACVLIPFNFFFR"
BASE COUNT         5594 a         2185 c         2815 g         4720 t
ORIGIN      
        1 aagatataag gtaggtaaat atggataaag aaactaatga caacgaatat agacgtcaaa
       61 gtgaacatcg cacttcggcg cctaagcgaa aaaagaagaa gaaaattagg aaattaccta
      121 tcattcttct gattgttgta attttactta tcgcattagt tgtatatatt gtgcatagtt
      181 acaatagcgg tgtagaatat gccaagaaac atgcgaaaga tgttaaagta catcaattta
      241 atggaccagt aaaaaatgat ggtaaaattt ctattcttgt actcggtgca gataaagcac
      301 aaggtggaca atcaagaaca gattctatca tggttgttca atatgacttt atcaataaaa
      361 agatgaaaat gatgtctgtc atgcgtgata tttatgcaga tattccagga tatggaaaac
      421 acaaaattaa ttcagcatac gctttaggtg gtccagagct acttagaaaa acacttgata
      481 aaaatttagg aattaatcct gaatattatg cagtagttga ttttactgga tttgagaaaa
      541 tgattgatga attaatgcca gaaggtgtac caattaatgt cgaaaaggat atgtcgaaaa
      601 atattggtgt atctttgaaa aagggtaacc ataggttgaa tggtaaagaa ttacttggtt
      661 atgcaagatt ccgtcacgac cctgaaggtg acttcggacg cgtgcgacgt cagcaacaag
      721 tgatgcaaac attgaaaaaa gaaatggtta attttagaac agttgttaaa ttaccaaaag
      781 ttgcaggtat tttaagaggc tatgtgaata caaacattcc tgattcaggg attttccaaa
      841 caggtttgag ttttggtatc cgaggtgaaa aagatgttaa gtcattgact gtgccaatca
      901 agaactcata cgaagatgtc aatacaaata ctgatggtag tgcattacag attaataaaa
      961 acacaaataa acaagctatt aaagactttt tagatgaaga ttaaaaataa acaaggcgat
     1021 ttctatcata ctcagataga agtcgccttg ttcgttttca aagaattaat tggcatgcat
     1081 cttatgaaga attattgatc tgactttctt acgccagcca caccataatg gtttggtttc
     1141 atttcttcta taacaacgtg aattgcttgt ctatttgccc ccgttgtttt ttctacggcg
     1201 tcagttactt cgctaactaa attttttaat tgttcatccg aacgaccttc taataatttt
     1261 acattgacga ttggcatcat ccaaacctcc taatcattat taatttgagt atatcatgta
     1321 atacttaaaa attcatttgc aaaataacag aacgtttgtt ctaaaatagt tgaaatggaa
     1381 cacgtgttcg tatataatga gattcaagta agggaggaat aacagtgtat aattatcatt
     1441 tattagaaga tagggatgtt ctatgtattg accaaaaaag tttttttgcg agtgtttctt
     1501 gtattgaaaa ggggctagat ccattagaaa caaagctagc tgttgttgca gatactaagc
     1561 gtcagggttc tgtaatattg gctgcgacac ctaaattaaa agaattaggc atcaagacag
     1621 ggtcgcgatt gtttgaaata ccacatagaa atgatattta cattatcaat ccaagtatgc
     1681 gtaaatatct taatgtttca gttgctattt ctaagattgc attgcgttat attccacctg
     1741 aagatttaca ccaatatagt attgacgaat tttttatgga tgttactgat agctatcata
     1801 gatttagttc tacagtacat gcattttgcg aaagacttaa acgtgaaatt tatgaagaaa
     1861 caggcattta ttgtactgtg ggcattggtt ctaatatgtt attaagtaaa attgctatgg
     1921 atgttgaagc gaagcatagt caaaatggta tagctgaatg gcgatatcaa gatgtaccaa
     1981 cgaaattatg gccaattcag cccttgcgag atttttgggg tattaatcgt cgaacagaag
     2041 ccaaattgaa taaaagagga atttttacta taggagattt agcgaaatat ccatataaat
     2101 ttttaaaaaa agagttcggt attttaggtg ttgatatgca tctacatgcg aatgggatag
     2161 atcagagtaa agtacgtgaa aagcacaaga tcagcaatcc atcgatatgc aaaagtcaaa
     2221 tattaatgag agattatcat tttgatgaag caaaagtagt aatgcaagag ttaattgaag
     2281 atgttgctag cagagttcga gcaagaaaaa aagtggcaag aacgatacat tttgcctttg
     2341 gctatagtga tgaaggcggt gtacataagc aatatacttt gaaagatcca acaaacttag
     2401 aaaaagatat ttataaagta gtaatgcatt tcgcagataa attatgtaat aaacaagcac
     2461 tatatcgtac gctaagtata tctttgagtc aatttattaa tgaggatgag cgacagttaa
     2521 gtctgtttga agatgaatac caacgcaaac gtgacgaatg tctagctaaa acgatagacc
     2581 aattacattt gaaatacggc aaaggtattg tgtccaaagc agtatcgttt acagaagcag
     2641 gtacaaaaca cggcagatta ggtttaatgg ctggacataa aatgtaatga ctatacggtt
     2701 taagtaatat ataactgtga ttcgtataaa ataagtctct aaagataaat atttcatata
     2761 tcacaataga tttcacaata atatctaaga atacatggaa tttatcaaaa gagacttaat
     2821 aattattgga tataacaatc aaaatcactc aatgcttgca taccgcgttc tcggtcagta
     2881 gggtttttga aactaatttt taaagcaccg tatatatctt cgcgtacttc taagattctt
     2941 aagttgctta tagatatgtt atgtaaactc aggatataag tcactttact tatcatacct
     3001 gattcatccg gaatgtctac atatagatca tacgcagtat ttagtccacc tagttgttta
     3061 gcgggtagtg cgtcgcgata cgatttagct tgggcaaaaa atgataacaa tttttcagaa
     3121 tcattgcttt caattagtct ttctaaatct tgaaactgac tttttagctg tcgaatcatt
     3181 tctaaaatat acgttttatt actcaaggtg atatctttcc acatttgtgc attactacta
     3241 gctatacgag tgatatcacg aaaaccacca gctgcaagtt tattaactaa atgatgttct
     3301 tgaccgttct tttgactaac atgaactaaa ctagatgcaa cgatatgagg taaatgactt
     3361 acgacgcttg ttacgtagtc gtgttcttca gcagtagtta caataaattt agcaagagta
     3421 ggtgataaca gttcttttaa cgtgtttgct gcttgctcat ttcttggctc attgtagact
     3481 aaaatataat aagcgttttc aaataagtgc tttttagcat ttagtacacc agatttatga
     3541 ctaccagcca ttggatgacc actgactaaa tgaatattat gctttaataa attgcattcg
     3601 tgttgctgta tcattgcttt agtactacca gtatcagaaa caataacacc aggtttagtt
     3661 ggcatatcta taagctcgct aagatattta tttgtgatag caacaggtgt tgcataaata
     3721 attacatcgg cttttttaat agcttcacta taatttaaac atttttcatt aataatgccg
     3781 attgatttag ctttatctaa ctgagaagta tctgcatcgt atgcaataat attagtatta
     3841 gggttatggt attttatatt gctagcaaga cttccaccaa ttaatccaag cccaacaaat
     3901 aaaactgttg tcatataaat caccttattt cgaaattttc agaataataa cattgtaaat
     3961 gagctgttga cacagtgcaa tagtaaataa aaatcgataa tagcattaat agataaacgg
     4021 agataaatca tctacaataa agagtatagt aacacaatgg caacggaggg gtaaatcaat
     4081 ggaaccaata ttagaaatga ttaaaacatt aacaggtatt aatagtcctt caggagacac
     4141 agaagaagca attcaatttg tcgaaaaata tgcaaaagac ttgggttatc aaacaacact
     4201 aacaaataaa ggtgcgttat taataacagt gccaggcaaa aatgatgaag tacaacgctg
     4261 tattactgct catgttgata ctttaggtgc aatggttaaa gaaattaaag aagatggtcg
     4321 cttagcaata gaattaattg gaggattcac gtataacgcg attgagggtg aatattgcca
     4381 aattaaaact gatgctggtc aaatatatac aggaacaatt tgtctgcatg aaacaagtgt
     4441 tcatgtatat agaaataatc atgaaatacc tagagatcaa aagcatatgg aaataagaat
     4501 tgatgaagta actacatcag aagaagatac aaagagttta ggtatttcag taggtgattt
     4561 tgttagcttt gatccacgta cagttatcac gtcatcaggt tttattaaat ctcgtcattt
     4621 agatgataaa gctagcgtag cgattatact acaattacta aagaaattaa aagaagagca
     4681 aataatatta ccacatacaa cgcaatttta tatttctaat aacgaagaaa taggttacgg
     4741 tgcaaatgca tcaattgatt cgaaaatcaa agaatatatt gcattagata tgggcgcgtt
     4801 gggagacggt caagcatcgg atgaatatac agtttctatt tgtgccaaag atgcttcagg
     4861 tccatatcat aagcaattga aatcgcacct agttaatctt tgcaaaataa ataacattcc
     4921 atataaagtc gacatatatc catattatgg ttcagatgct tcagcagctt tacatgctgg
     4981 tgcggatatc agacatggtt tatttggcgc tggcattgaa tcatctcatg caatggaacg
     5041 aacacatatt gattctatta aagcgacaga gaaattacta tatgcatatt gcttatcacc
     5101 aattgagtaa acaattagtg ttgacaaatg tgaacgacct atgtaatata atgaactata
     5161 aaaataatta gaattttcta aagaaatagt agcagatatg aaacgtagca aatagaaagc
     5221 taatgggtga tgggaattag cacgccatat cttgtgaatt ggactttgga aaacaattga
     5281 atgagttttg aaagtgaaca tgaattatgt taactaaggt ggcaccacgg taacgcgtcc
     5341 ttacaggtat atgcgttatg tggtgtcttt ttatttagac aaaatgtagt agttaattaa
     5401 aggtagcaac agaaagttag tggatgatgt gaactaacac cgagattaat gaaattgggt
     5461 tttgtctgca acagaaaaat tatatatagt aaagagtgaa ctatgaatat ttcgaatatt
     5521 cggttaattt aggtggtacc acgcgtcagc gtcctttata ttgataagga tgctggcgct
     5581 tttttgaaag gagcgtatag aatggatata ttttataaaa aaataaaagc aaatgtaacg
     5641 ccagaagttt tagcacaact tcattccaag aagatcattt tggaaagtac aaatcaacaa
     5701 caaactaaag gtcgctattc agttgttatt tttgatattt atggcacttt aactttagat
     5761 aatgatgtat tatcagtaag cactttaaaa gaatcgtatc aaatcagtga aagaccgtac
     5821 cattatttaa cgactaaaat aaatgaagac taccataata ttcaagatga gcaacttaag
     5881 tcattaccgt ttatatctgg atatgttggg acgtgtagct ttgatttagt aagacatgaa
     5941 tttcctaaat tgcaatcaat acaattagaa gatcacaagc agcacgatgt aaggttatat
     6001 atggttgaac aagtttatgt atttgaccat tacaaagatg agttatatat catcgcgacg
     6061 aatcaatttt caaattcaac aaaatcagat cttgagaatc gagttaataa gtctatcgaa
     6121 gacttaacta aaatccaacc attcatgcct acacaagatt ttgattttaa aactaaagaa
     6181 attcaatcaa acatttctga agaaagattt atcgaaatga ttcagtattt caaagagaaa
     6241 ataacagaag gggatatgtt tcaagttgtg ccatcaagaa tttacaaata tgcgcatcat
     6301 gctagtcagc atttaaatca actttcgttt caactgtatc aaaatttaaa acgacaaaac
     6361 ccaagtccat atatgtatta tcttaatatc gatcaaccat atattgtcgg tagttctccc
     6421 gaaagtttcg taagtgtcaa agatcaaatt gtaacaacta atcctattgc aggtacgatt
     6481 caacgtggtg agacgacaca aatagataat gagaatatga aacaactact taatgatcca
     6541 aaagaatgca gcgaacatcg tatgctagtt gatttagggc gtaatgatat tcatagagta
     6601 agtaaaatcg gtacctcaaa aattactaaa ttaatggtta ttgaaaaata tgaacatgtt
     6661 atgcatatcg taagtgaagt cacaggtaaa ataaatcaaa atttatcgcc aatgacagtt
     6721 attgcgaatt tattaccaac aggtaccgtt tcaggtgcac caaaattacg tgcaattgaa
     6781 agaatatatg aacaatatcc acataaacgg ggcgtttata gtggtggtgt tggatacata
     6841 aattgtaatc ataacttaga ttttgcatta gcaattcgaa cgatgatgat agatgagcag
     6901 tatatcaacg tagaagctgg ttgtggcgtt gtatatgatt ctattcctga aaaagaactg
     6961 aatgaaacga aattgaaagc taaaagctta ttggaggtga gcccatgatc ttagttgtag
     7021 ataattatga ttcctttaca tataacctag tggatattgt tgctcaacat actgacgtca
     7081 ttgttcaata ccctgatgat gataatgtgc tgaatcaatc ggtggacgct gttattatat
     7141 ctcctggtcc agggcatcca ttagacgatc aacagttgat gaaaatcata tcaacctatc
     7201 aacacaaacc cattttaggt atatgtttag gggctcaggc actgacttgt tactacggtg
     7261 gagaagtcat taaaggcgac aaggttatgc acggcaaagt tgatacacta aaggttatat
     7321 cgcatcatca acatctgtta tatcaagata taccagaaca gttttcaatt atgagatatc
     7381 attcattaat aagtaaccct gacaattttc cagaagaatt gaaaattact ggacgtacca
     7441 aagattgtat acagtcattc gagcataaag aaagaccgca ttatggtatt cagtaccatc
     7501 ctgaatcatt tgctacagac tatggtgtca aaataattac aaatttcatt aatctagtga
     7561 aggaaggatg aaaaccatga cattactaac aagaataaaa actgaaacta tattacttga
     7621 aagcgacatt aaagagctaa tcgatatact tatttctcct agtattggaa ctgatattaa
     7681 atatgaatta cttagttcct attcggagcg agaaatccaa caacaagaat taacatatat
     7741 tgtacgtagc ttaattaata caatgtatcc acatcaacca tgttatgaag gggctatgtg
     7801 tgtgtgcggc acaggtggtg acaagtcaaa tagtttcaac atttcaacga ctgttgcttt
     7861 tgttgtagca agtgctggcg taaaagttat aaaacatggt aataaaagta ttacctcaaa
     7921 ttcaggtagt acggatttgt taaatcaaat gaacatacaa acaacaactg ttgatgatac
     7981 acctaaccaa ttaaatgaaa aagaccttgt attcattggt gcaactgaat catatccaat
     8041 catgaagtat atgcaaccag ttagaaaaat gattggaaag cctacaatat taaaccttgt
     8101 gggtccatta attaatccat atcacttaac gtatcaaatg gtaggcgtct ttgatcctac
     8161 aaagttaaag ttagttgcta aaacgattaa agatttaggt agaaaacgtg caatcgtttt
     8221 acatggtgca aatggtatgg atgaagcaac actatctggt gataatttga tatatgaatt
     8281 gactgaagat ggagaaatca aaaattacac attaaatgcg actgattatg gtttgaaaca
     8341 tgcgccgaat agtgatttta aaggcggttc acctgaagaa aatttagcaa tctcccttaa
     8401 tatcttgaat ggtaaagatc agtcaagtcg acgtgatgtt gtcttactaa atgcgggttt
     8461 aagcctttat gttgcagaga aagtggatac catcgcagaa ggcatagaac ttgcaactac
     8521 attgattgat aatggtgaag cattgaaaaa ataccatcaa atgagaggtg aataatatga
     8581 cgattttatc agaaattgtt aaatataaac agtcactttt acaaaatggc tattatcaag
     8641 acaaacttaa taccttgaaa agtgtgaaga ttcagaataa aaaatctttt ataaacgcaa
     8701 ttgagaaaga accaaagcta gcaattattg cagaaattaa atcgaagagt cctacagtta
     8761 atgacttacc tgaacgagat ttatcgcaac aaatctcaga ttatgaccaa tatggtgcaa
     8821 atgccgtgtc cattttaact gatgaaaagt actttggtgg tagttttgaa agattacaag
     8881 cattgacgac aaaaacaaca ttacccgtat tatgcaaaga ctttattata gacccgcttc
     8941 aaattgatgt tgctaaacaa gctggtgcat ctatgatttt attgatcgtt aacatcttat
     9001 ctgataaaca attgaaagat ttatataact acgctatatc gcaaaatcta gaagtgttag
     9061 ttgaagtaca tgatcgccat gaattagaac gtgcctataa ggttaatgct aaattgattg
     9121 gtgtaaataa cagggactta aaacgatttg ttacaaatgt ggaacataca aatactattt
     9181 tagaaaataa aaaaacaaat cattattata tttctgaaag tggtattcac gatgcatctg
     9241 atgtaagaaa aatcttgcat agtggtatcg atggcttact aataggtgag gcgcttatgc
     9301 gttgtgacaa tctatctgaa tttttaccac aactgaaaat gcaaaaggtg aagtcatgat
     9361 gaaattgaaa ttttgtggct ttacatcaat aaaggatgtt acagcggcca gtcaattacc
     9421 tattgatgcg ataggtttca tccattatga aaaaagtaaa aggcatcaaa caattaccca
     9481 aataaaaaag ttagcgtctg ctgttccaaa tcatatcgat aaagtatgtg tcatggtaaa
     9541 tcctgattta acaacaattg aacacgtatt aagcaatacg tcaattaaca caatacagtt
     9601 acacggcaca gaatctattg attttataca ggaaattaaa aagaaatatt caagcattaa
     9661 aatcactaaa gctttagctg cagatgaaaa cataatccaa aacataaata aatataaagg
     9721 gttcgtagat ttatttatta tcgacacacc ctcagtgtcg tatggtggta ccggtcaaac
     9781 atatgactgg actattttga agcacataaa agacatacct tatttgatag caggaggcat
     9841 taactctgaa aatattcaaa cagttaatca acttaaatta tcacatcaag gttatgatct
     9901 tgcatcaggt atagaagtaa atgggcgaaa agatatagaa aaaatgacag caattgtaaa
     9961 tattgtgaaa ggagatagag acaatgaata aacaaataca aacagaagca gatgaattag
    10021 gtttctttgg tgaatatgga gggcaatatg ttccagaaac attaatgcca gcaattattg
    10081 agttgaaaaa agcttataaa gaggcaaaag cagacccaga gtttcaaaga gaactggaat
    10141 actatttatc agagtatgta ggacgcgcga caccacttac atatgctgca tcatatactg
    10201 aaagcctagg tagcgctaaa atatatttga aacgagagga tctaaatcat acaggcgccc
    10261 ataaaattaa taatgcgtta ggtcaagcgt tgcttgctaa aagaatgggc aagaagaagc
    10321 ttgttgctga aactggtgcg ggtcaacatg gtgtagctag tgctacggtt gctgcattat
    10381 ttgatatgga acttgttgtc tttatgggaa gtgaagatat taaaagacaa caacttaatg
    10441 tatttagaat ggaattactt ggtgcaaagg ttgtggcagt tgaagatggt caagggactt
    10501 tatcggatgc agttaataaa gcattgcaat attgggtaag tcatgtagat gatacacatt
    10561 atttattagg ttctgcatta ggtccagacc cgttcccaac gattgttaga gattttcaga
    10621 gagtgattgg taaagaaata aaatcacaga tattgaagaa agaaggtcga cttccggatg
    10681 caattgtagc atgtatcggt ggtggctcaa atgcaatcgg tacattttat ccatttatta
    10741 aagatgatgt tgcattatac ggtgttgaag ccgcaggtca aggcgatgat actgataaac
    10801 atgcacttgc aattggcaaa ggatcacctg gcgtattaca tggtactaaa atgtatttaa
    10861 ttcaagatga agatgggcaa gtgcaactag cacattctat ttcagcagga cttgattatc
    10921 ctggtattgg accagaacat tcttattacc acgacattgg tagagtaact tttgaaaatg
    10981 ctagtgatac acaagcaatg aatgctttaa tcaactttac aaaacatgaa ggtattatac
    11041 ctgcaattga aagtgcacat gcactgagtt atgttgaaag actagcgcct acgatgtcga
    11101 aagaagatat tattgtagta actatttctg gacgtggcga taaagatatg gaaacaatta
    11161 gacaatatat ggtagagcga ggtcttgcaa atgactaaat tatttatacc ttatattatg
    11221 ggcaataaag atttgattga aaatgcaaca ttgttgagtg aaaatggtgc agatataatt
    11281 gaaattggag tacctttctc tgatccggtt gctgatggtc cagttatcat ggaagcaggt
    11341 caacaagcga ttaaacaagg catcacgata gattatattt tcaatcaatt agaaaaacat
    11401 ggtgatcaaa ttaagtgtaa ctatgtatta atgacgtatt ataatattat ttgtcattat
    11461 ggagaacaag cgttttttga aaaatgtcga gatactggtg tctacggctt aattattcct
    11521 gatttaccat atgaattatc gcagcgttta aaacaacaat ttagtcacta tggcgtcaaa
    11581 atcatatcgt tagttgcgat gactactgat gacaaacgta taaaagatat cgtatcccat
    11641 gcggaaggct ttatttatac tgtgacgatg aatgcgacaa cagggcaaaa cggtgcgttt
    11701 catccagaat taaaacgaaa aattgagtca attaaagcga tagccaatgt gccagttgtc
    11761 gcaggatttg gtataagaac accacaacat gttgcagata taaaagaggt tgcagatggc
    11821 attgtcattg gtagcgaaat cgttaagcga tttaaatcta acacgcgtga ggaaatcatt
    11881 aaatatttac aatctatcca acaaacattg aataattaag tttacttgat ttaaaaaaat
    11941 taggcgaata ctgtttgaaa aagtgaaaaa cggtgaatta taaaattgaa tagaaatgat
    12001 acaaaagtaa tatgagcaaa cccaaacgtt catattactt tttttgaaat tgtatgcaaa
    12061 aatctaaata ttactataaa agtatacgca attaaagcgt ttatgtttta gttttaacat
    12121 taactattgt atacttattt agattagatt tattattttt gacatttgca gaggggaaat
    12181 agaaaaactg caaatacgga aatgaaatta attaacgaga gacaaatagg agtaatgata
    12241 atgaagttta caaatttaac agctaaagag tttggtgcat ttacagatag catgccatac
    12301 agtcatttca cgcaaactgt tggccactat gagttaaagc ttgctgaagg ttatgaaaca
    12361 catttagtgg gaataaaaaa caataataac gaggtcattg cagcttgctt acttactgct
    12421 gtacctgtta tgaaagtgtt caagtatttt tattcaaatc gcggtccagt gattgattat
    12481 gaaaatcaag aactcgtaca ctttttcttt aatgaattat caaaatatgt taaaaaacat
    12541 cgttgtctat acctacatat cgatccatat ttaccatatc aatacttgaa tcatgatggc
    12601 gagattacag gtaatgctgg taatgattgg ttctttgata aaatgagtaa cttaggattt
    12661 gaacatactg gattccataa aggatttgat cctgtgctac aaattcgtta tcactcagtg
    12721 ttagatttaa aagataaaac agcagatgac atcattaaaa atatggatgg acttagaaaa
    12781 agaaacacga aaaaagttaa aaagaatggt gttaaagtaa gatttttatc tgaagaagaa
    12841 ctaccaattt ttagatcatt tatggaagat acgtcagaat caaaagcttt tgctgatcgt
    12901 gatgacaaat tttactacaa tcgcttaaaa tattacaaag accgtgtgtt agtaccttta
    12961 gcgtatatca actttgatga atatattaaa gaactaaacg aagagcgtga tattttaaat
    13021 aaagatttaa ataaagcgtt aaaggatatt gaaaaacgtc ctgaaaataa aaaagcacac
    13081 aacaagcgag ataacttaca acaacaactt gatgctaatg agcaaaagat tgaagaaggt
    13141 aaacgtctac aagaagaaca tggtaatgaa ttacctatct ctgctggttt cttctttatc
    13201 aacccatttg aagttgttta ttatgctggt ggtacatcaa atgctttccg tcattttgcc
    13261 ggaagttatg cagtgcaatg ggaaatgatt aattatgcat taaatcatgg cattgaccgt
    13321 tataatttct atggtgttag tggtaaattt acagaagatg ctgaagatgc tggtgtagtt
    13381 aaattcaaaa aaggttacaa tgctgaaatt attgaatatg ttggtgactt tattaaacca
    13441 attaataaac ctgtttacgc agcatatacc gcacttaaaa aagttaaaga cagaattttt
    13501 taggaaggga attatcaaaa catgaaattt acagagttaa ctgttaccga atttgacaac
    13561 tttgtacaaa atccatcatt ggaaagtcat tatttccaag taaaagaaaa tatagttacc
    13621 cgtgagaatg atggctttga agtagtttta ttaggtatta aagacgacaa taacaaagta
    13681 attgcagcaa gccttttctc taaaattcct actatgggaa gttatgttta ctattcgaat
    13741 cgtggtccag taatggattt ttcagattta ggattagttg attattattt aaaagagtta
    13801 gataaatatt tacagcaaca tcaatgttta tatgttaaat tagatccgta ttggttatat
    13861 catctatatg ataaagatat cgtgccattt gaaggtcgcg agaaaaatga tgccctagta
    13921 aacttgttta aatcacatgg ttacgagcat catggcttta caactgagta tgatacatcg
    13981 agccaagtac gatggatggg cgtattaaac cttgaaggta aaacacccga aacattgaaa
    14041 aagacatttg atagtcaacg taaacgtaat attaataaag cgataaacta tggtgttaaa
    14101 gtcagattcc ttgaacgtga tgagttcaat cttttcctag atttatatcg tgaaactgaa
    14161 gagcgtgctg gatttgtatc aaaaacagat gattattttt ataactttat tgacacatat
    14221 ggagataaag tattagtacc attagcatat attgaccttg atgaatatgt gttaaagctg
    14281 caacaggaat tgaatgacaa agaaaatcgt cgtgatcaaa tgatggcgaa agaaaacaaa
    14341 tcagataaac aaatgaagaa aattgcagaa ttagataagc aaattgatca tgatcagcat
    14401 gaattattga atgcaagtga attgagcaaa acggacggcc caattctaaa ccttgcttct
    14461 ggtgtttatt ttgcaaatgc atatgaagtg aattatttct ctggtggttc atcagaaaaa
    14521 tataatcaat ttatgggacc atacatgatg cattggttta tgattaacta ttgcttcgat
    14581 aatggttatg atcgttataa tttctatggt ttatcaggtg attttacgga aaacagtgaa
    14641 gattatggcg tataccgctt taaacgtgga tttaatgtac aaatcgaaga attaataggg
    14701 gatttctata aaccaattca taaagtgaaa tattggttgt tcacaacatt ggataaacta
    14761 cgtaaaaaat taaagaaata gatgtaaatg atgttagggc ttatagttat tgatactata
    14821 ggctcttttt tatgcttttt ttagcgaaat tttaattatt tcacactaag aaaaactagt
    14881 atgtatttgt caatctaatg tttttcatta taatgatgtt ttattgattt agttttatga
    14941 ttaaaagatt atgcaaaaca taaaatatat agcgtcatat ttgagtttta atgataatat
    15001 ttttataata tataatataa aataatggtt atctgagtat gaaatgcaag taagtgaggt
    15061 agttaaaaaa tgaatcgaat tgaaaaacat gccaaaaaca cttttattat tctgatgctc
    15121 attatgttat tttggatttt tatgagtttt attttccaga aattattatt tccaccttcg
    15181 aaaaataatt taactacata tgaagcatta aaatattata cacatttaaa gggatattac
    15241 ggtttggatc atatatcaaa aggaatagca tacattgcct gtgtgttaat tccattcaat
    15301 ttctttttta gatt
//
LOCUS       DAMZKC010000051        15304 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.051, whole genome shotgun sequence.
ACCESSION   DAMZKC010000051 DAMZKC010000000
VERSION     DAMZKC010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 15304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15304
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.051"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(192..1190)
                     /gene="sirC"
                     /locus_tag="POB55_001207"
     CDS             complement(192..1190)
                     /gene="sirC"
                     /locus_tag="POB55_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter permease
                     subunit SirC"
                     /protein_id="HDI6500178.1"
                     /translation="MTEKINKKDNYHLIFALIFLAIVSVVSMMSGSSFIPLQRVLMYF
                     INPNDSMDQFTLEVLRLPRITLAILAGAALGMSGLMLQNVLKNPIASPDIIGITGGAS
                     LSAVVFIAFFSHLTIHLLPLFAVLGGAIAMMILLVFQTKGQIRPTTLIIIGISMQTLF
                     IALVQGLLITTKQLSAAKAYTWLVGSLYGATFKDTIILGMVILAVVPLLFLVIPKMKI
                     SILDDPVAIGLGLHVQRMKLIQLITSTILVSMAISLVGNIGFVGLIAPHIAKTIVRGS
                     YAKKLLMSAMIGAISIVIADLIGRTLFLPKEVPAGVFIAAFGAPFFIYLLLTVKKL"
     gene            complement(1187..2182)
                     /gene="sirB"
                     /locus_tag="POB55_001208"
     CDS             complement(1187..2182)
                     /gene="sirB"
                     /locus_tag="POB55_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter permease
                     subunit SirB"
                     /protein_id="HDI6500179.1"
                     /translation="MLLKPKYQIVIAGLCLAIVAILSLMIGNTLVSPGTVIQALFNFD
                     SENDLHDVVTGARASRTIIALLTGAALAVSGLLMQALTRNPIASPGLFGVNAGAVFFV
                     IFSITFIQIQSFKMIVVIAFLGAIVVTVLVVALGMFRQTLFSPHRVILAGAAIAMLFT
                     AFTQGILIMNETDLQGLLFWLSGSVSLRNIWDIPWIIPLVLILILIAFSMAAHINILM
                     TSDDIAIGLGQNIKLIKWMIIMLISMLAGISVAVAGAIVFVGLIVPNISKRLLPPNYK
                     YLIPFTALAGAILMIISDIVARIIIKPLELPIGVVTAVIGAIVLIYIMKKGRQRL"
     gene            complement(2198..3190)
                     /gene="sirA"
                     /locus_tag="POB55_001209"
     CDS             complement(2198..3190)
                     /gene="sirA"
                     /locus_tag="POB55_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter
                     substrate-binding protein SirA"
                     /protein_id="HDI6500180.1"
                     /translation="MNKVIKMLVVTLAFLLVLAGCSGNSNKQSSDNKDKETTSIKHAM
                     GTTEIKGKPKRVVTLYQGATDVAVSLGVKPVGAVESWTQKPKFEYIKNDLKDTKIVGQ
                     EPAPNLEEISKLKPDLIVASKVRNEKVYDQLSKIAPTVSTDTVFKFKDTTKLMGKALG
                     KEKEAEDLLKKYDDKVAAFQKDAKAKYKDAWPLKASVVNFRADHTRIYAGGYAGEILN
                     DLGFKRNKDLQKQVDNGKDIIQLTSKESIPLMNADHIFVVKSDPNAKDAALVKKTESE
                     WTSSKEWKNLDAVKNNQVSDDLDEITWNLAGGYKSSLKLIDDLYEKLNIEKQSK"
     gene            3421..4401
                     /gene="sbnA"
                     /locus_tag="POB55_001210"
     CDS             3421..4401
                     /gene="sbnA"
                     /locus_tag="POB55_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000570808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diaminopropionate biosynthesis protein SbnA"
                     /protein_id="HDI6500181.1"
                     /translation="MIEKSQACHDSLLDSVGQTPMVQLHQLFPKHEVFAKLEYMNPGG
                     SMKDRPAKYIIEHGIKHGLITENTHLIESTSGNLGIALAMIAKIKGLKLTCVVDPKIS
                     PTNLKIIKSYGANVEMVEEPDAHGGYLMTRIAKVQELLATIDDAYWINQYANELNWQS
                     HYHGAGTEIVETIKQPIDYFVAPVSTTGSIMGMSRKIKEVHPNAQIVAVDAKGSVIFG
                     DKPINRELPGIGASRVPEILNRSEINQVIHVDDYQSALGCRKLIDYEGIFAGGSTGSI
                     IAAIEQLITSIEEGATIVTILPDRGDRYLDLVYSDTWLEKMKSRQGVKSE"
     gene            4398..5408
                     /gene="sbnB"
                     /locus_tag="POB55_001211"
     CDS             4398..5408
                     /gene="sbnB"
                     /locus_tag="POB55_001211"
                     /EC_number="1.5.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-[(2S)-2-amino-2-carboxyethyl]-L-glutamate
                     dehydrogenase SbnB"
                     /protein_id="HDI6500182.1"
                     /translation="MNREMLYLNRSDIEQAGGNHSQVYVDALTEALTAHAHNDFVQPL
                     KPYLRQDPENGHIADRIIAMPSHIGGEHAISGIKWIGSKHDNPSKRNMERASGVIILN
                     DPETNYPIAVMEASLISSMRTAAVSVIAAKHLAKKGFKDLTIIGCGLIGDKQLQSMLE
                     QFDHIERVFVYDQFSEACARFVDRWQQQRPEINFIATENAKEAVSNGEVVITCTVTDQ
                     PYIEYDWLQKGAFISNISIMDVHKEVFIKADKVVVDDWSQCNREKKTINQLVLEGKFS
                     KEALHAELGQLVTGDIPGREDDDEIILLNPMGMAIEDISSAYFIYQQAQQQNIGTTLN
                     LY"
     gene            5429..7183
                     /gene="sbnC"
                     /locus_tag="POB55_001212"
     CDS             5429..7183
                     /gene="sbnC"
                     /locus_tag="POB55_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis protein SbnC"
                     /protein_id="HDI6500183.1"
                     /translation="MQNHTAVNTAQTIILRDLVDALLFEDIAGIVSNSDITKENGQTL
                     LIYERETQQIKIPVYFSALNMFRYESSQPITIEGRASKQPLTAAEFWQTIANMNCDLS
                     HEWEVARVEEGLTTAATQLAKQLSELDLASHPFVMSEQFASLKDRPFHPLAKEKRGLR
                     EADYQVYQAELNQSFPLMVAAVKKTHMIHGDTANIDELENLTAPIKEQATDMLNDQGL
                     SIDDYVLFPVHPWQYQHILPNVFAKEISEKLVVLLPLKFGDYLSSSSMRSLIDIGAPY
                     NHVKVPFAMQSLGALRLTPTRYMKNGEQAEQLLRQLIEKDEALAKYVMVCDETAWWSY
                     MGQDNDIFKDQLGHLTVQLRKYPEVLAKNDTQQLVSMAALAANDRTLYQMICGKDNIS
                     KNDVMTLFEDIAQVFLKVTLSFMQYGALPELHGQNILLSFEDGRVQKCVLRDHDTVRI
                     YKPWLTAHQLSLPKYVVREDTPNTLINEDLETFFAYFQTLAVSVNLYAIIDAIQDLFG
                     VSEHELMSLLKQILKNEVATISWVTTDQLAVRHILFDKQTWPFKQILLPLLYQRDSGG
                     GSMPSGLTTVPNPMVTYD"
     gene            7176..8432
                     /gene="sbnD"
                     /locus_tag="POB55_001213"
     CDS             7176..8432
                     /gene="sbnD"
                     /locus_tag="POB55_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B export MFS transporter"
                     /protein_id="HDI6500184.1"
                     /translation="MINQSIWRSNFRILWLSQFIAIAGLTVLVPLLPIYMASLQNLSV
                     VEIQLWSGIAIAAPAVTTMIASPIWGKLGDKISRKWMVLRALLGLAVCLFLMALCTTP
                     LQFVLVRLLQGLFGGVVDASSAFASAEAPAEDRGKVLGRLQSSVSAGSLVGPLIGGVT
                     ASILGFSALLMSIAVITFIVCIFGALKLIETTHMPKSQTPNINKGIRRSFQCLLCTQQ
                     TCRFIIVGVLANFAMYGMLTALSPLASSVNHTAIDDRSVIGFLQSAFWTASILSAPLW
                     GRFNDKSYVKSVYIFATIACGCSAILQGLATNIEFLMAARILQGLTYSALIQSVMFVV
                     VNACHQQLKGTFVGTTNSMLVVGQIIGSLSGAAITSYTTPATTFIVMGVVFAVSSLFL
                     ICSTITNQINDHTLMKLWELKQKSAK"
     gene            8422..10158
                     /gene="sbnE"
                     /locus_tag="POB55_001214"
     CDS             8422..10158
                     /gene="sbnE"
                     /locus_tag="POB55_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2,3-diaminopropanoate--citrate ligase SbnE"
                     /protein_id="HDI6500185.1"
                     /translation="MQNKELIQHAAYAAIERILNEYFREENLYQVPPQNHQWSIQLSE
                     LETLTGEFRYWSAMGHHMYHPEVWLIDGKSKKITTYKEAIARILQHMAQSADNQTAVQ
                     QHMAQIMSDIDNSIHRTARYLQSNTIDYVEDRYIVSEQSLYLGHPFHPTPKSASGFSE
                     ADLEKYAPECHTSFQLHYLAVHQDVLLTRYVEGKEDQVEKVLYQLADIDISEIPKNFI
                     LLPTHPYQINVLRQHPQYMQYSEQGLIKDLGVSGDSVYPTSSVRTVFSKALNIYLKLP
                     IHVKITNFIRTNDLEQIERTIDAAQVIASVKDEVETPHFKLMFEEGYRALLPNPLGQT
                     VEPEMDLLTNSAMIVREGIPNYHADKDIHVLASLFETMPDSPMSKLSQVIEQSGLAPE
                     AWLECYLNRTLLPILKLFSNTGISLEAHVQNTLIELKDGIPDVCFVRDLEGICLSRTI
                     ATEKQLVPNVVAASSPVVYAHDEAWHRLKYYVVVNHLGHLVSTIGKATRNEVVLWQLV
                     AHRLMTWKKEYANNAVFVDCVEDLYQTPTIAAKANLMSKLNDCGANPIYTHIPNPICH
                     NKEVSYCESNNS"
     gene            10067..11917
                     /gene="sbnF"
                     /locus_tag="POB55_001215"
     CDS             10067..11917
                     /gene="sbnF"
                     /locus_tag="POB55_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(L-alanin-3-ylcarbamoyl)-2-[(2-
                     aminoethylcarbamoyl)methyl]-2-hydroxypropanoate synthase
                     SbnF"
                     /protein_id="HDI6500186.1"
                     /translation="MIVVQTLFIHIYQIQFVITRRYRIVNQTILNRVKTRVMHQLVSS
                     LIYENIVVYKASYQDGAGHFTIEGHDSEYRFTAEKTHSFDRIRITSPIERVVGDEADT
                     TTDYTQLLREVVFTFPKNDEKLEQFIVELLQTELKDTQSMQYRESNPPATPETFNDYE
                     FYAMEGHQYHPSYKSRLGFTLSDNLKFGPDFVPNVKLQWLAIDKDKVETTVSRNVVVN
                     EMLRQQVGDKTYEHFVQQIEASGKHVNDVEMIPVHPWQFEHVIQVDLAEERLNGTVLW
                     LGESDELYHPQQSIRTMSPIDTTKYYLKVPISITNTSTKRVLAPHTIENAAQITDWLK
                     QIQQQDTYLKDELKTAFLGEVLGQSYLNTQLSPYKQTQVYGALGVIWRENIYHMLIDE
                     EDAIPFNALYASDKDGVPFIENWIKQYGSEAWTKQFLAVAIRPMIHMLYYHGIAFESH
                     AQNMMLIHENGWPTRIALKDFHDGVRFKREHLSEAASHLTLKPMPEAHKKVNSNSFIE
                     TDDERLVRDFLHDAFFFINIAEIILFIEKQYGIDEQRQWQWVKDIIEAYQEAFPELNN
                     YQHFDLFEPTIQVEKLTTRRLLSDSELRIHHVTNPLGVGGINDATTISET"
     gene            11892..12668
                     /gene="sbnG"
                     /locus_tag="POB55_001216"
     CDS             11892..12668
                     /gene="sbnG"
                     /locus_tag="POB55_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis citrate synthase
                     SbnG"
                     /protein_id="HDI6500187.1"
                     /translation="MQQLSLKHRLNNGDSVYGIFNSIPDPLMIEVIAASGYDFVVIDT
                     EHVAINDETLAHLIRAAEAAHIIPIVRVTAVIDRDIIKVLDMGARGIIVPHVKDRETV
                     EHIVKLSRYYPQGLRSLNGGRMARFGRTPLLDAMEMANEHIMVIAMIEDVEGVMAIDD
                     IAQVEGLDMIVEGAADLSQSLGIPWQTRDDQVTSHVQHIFEVVNAHGKHFCALPREDE
                     DIAKWQAQGVQTFILGDDRGKIYRHLSASLATSKQKGDDG"
     gene            12668..13870
                     /gene="sbnH"
                     /locus_tag="POB55_001217"
     CDS             12668..13870
                     /gene="sbnH"
                     /locus_tag="POB55_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis decarboxylase
                     SbnH"
                     /protein_id="HDI6500188.1"
                     /translation="MRIVQPVIEQLKAQSHPVCHYIYDLVGLEHHLQHITLSLPSNCQ
                     MYYAMKANSERTILDTISQYVEGFEVASQGEIAKGLAFKPANHIIFGGPGKTDEELRY
                     AVSEGVQRIHVESMHELQRLNAILEDEDKTQHILLRVNLAGPFPNATLHMAGRPTQFG
                     ISEDEVDDVIEAALAMPNIHLDGFHFHSISNNLDSNLHVDVVKLYFKKAKSWSEKHRF
                     PLKHINLGGGIGVNYADLTSQFEWDNFVENFKTLIVEQEMEDVTLNFECGRFIVAHIG
                     YYVTEVLDIKKVHGAWYAILRGGTQQFRLPVSWQHNHPFEIYRYKDNPYSFEKVSISR
                     QDTTLVGQLCTPKDVFAREVQIDAISTGDVIVFKYAGAYGWSISHHDFLSHPHPEFIY
                     LTQTKEDE"
     gene            13874..14638
                     /gene="sbnI"
                     /locus_tag="POB55_001218"
     CDS             13874..14638
                     /gene="sbnI"
                     /locus_tag="POB55_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transcriptional
                     regulator/O-phospho-L-serine synthase SbnI"
                     /protein_id="HDI6500189.1"
                     /translation="MNHIHEHLKLVPVDKIDLHETFEPLRLEKTKSSIEADDFIRHPI
                     LVTAMQHGRYMVIDGVHRYTSLKALGCKKVPVQEIHETQYSISTWQHKVPFGVWWETL
                     QQEHRLPWTTETRQEAPFITMCHGDTEQYLYTKDLGEAHFQVWEKVVASYSGCCSVER
                     IAQGTYPCLSQQDVLMKYQPLSYKEIEAVVHKGETVPAGVTRFNISGRCLNLQVPLAL
                     LKQDDDVEQLRNWKQFLADKFANMRCYTEKVYLVEQ"
     gene            14834..15241
                     /locus_tag="POB55_001219"
     CDS             14834..15241
                     /locus_tag="POB55_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500190.1"
                     /translation="MSSIIGKIAIWIGIVAQIYFSVVFVRMISINIAGGSDYETIFLL
                     GLILALFTVLPTIFTAIYMESYSVIGGALFIVYAIIALCLYNFFSSILWLIGGILLIW
                     NKYSKDESTDENEKVDIESTENQFESKDKITKE"
BASE COUNT         5095 a         2472 c         3056 g         4681 t
ORIGIN      
        1 cgaagaatat tgattgatat tatagaatat gagtcggtgt tatttgtata taaagtatta
       61 atatgtaatt aagaaagtcg acgcttgtta acaataacaa caactatatg tataaaaaaa
      121 cctcgcaact gttagttaac tgaatcattt agctaacttc gttgtgaggt cattttgttt
      181 taataatatc gttataactt tttcacggtt aataataagt atatgaagaa tggggcacca
      241 aaagcagcaa taaatacacc tgctggcact tctttaggca agaataaggt acgcccaatt
      301 aagtctgcaa taacaattga tatggcacca atcattgctg acattagtaa ctttttagca
      361 taacttccgc gaacgattgt tttcgcgata tgtggtgcga ttaaaccgac aaacccaatg
      421 ttacctacta aactgattgc catagatacg agtatagtag aagtgattaa ttggattagt
      481 ttcatacgtt gtacatgtaa gcctaagcca atcgctacag ggtcatcaag tatagatatt
      541 ttcatttttg gtataacaag aaataacaac ggcacaacag ctaaaataac catacccaag
      601 atgattgtat ctttaaacgt agcaccgtaa agacttccga ctagccatgt ataagctttg
      661 gcagcagata attgcttcgt tgtaatgagt aatccttgga caagcgcaat aaacaacgtt
      721 tgcatcgaaa taccgatgat tatgagtgtt gtcgggcgta tttgtccttt cgtttgaaac
      781 actaatagta tcatcattgc aattgcgcca cctaatacag cgaatagtgg aagtaaatgt
      841 atcgttaaat ggctgaaaaa tgcaataaag acaacggcac ttaagctagc accacctgtg
      901 ataccgataa tatcaggtga ggcaattgga ttttttaata cattttgcaa cattaaacca
      961 ctcattccta gtgcggcacc tgctaaaatc gcaagtgtaa tgcgaggtaa gcgtaatact
     1021 tctaaagtga attgatccat actgtcattt ggatttataa agtacatcag tacgcgttgt
     1081 aatggtataa agcttgaacc actcatcata cttaccactg aaacgatggc taaaaagatt
     1141 aacgcgaaga tgagatggta attgtctttt ttattaatct tttcggtcat aagcgttgac
     1201 gtcctttctt cataatatag attaagacaa tagcgccaat gacagcggta acgacaccga
     1261 taggcaactc tagtggctta attattatac gagcaacaat gtctgaaatg atcattagga
     1321 ttgctccagc taatgcagta aaaggaatta aatacttata gtttggtggt aataatcgtt
     1381 tgctaatatt cggtacgata agacccacaa agacgattgc tccagctacg gctaccgaaa
     1441 taccggctaa catactgatg agcataataa tcatccattt gattaatttt atgttttgac
     1501 cgaggccgat tgcaatgtcg tcacttgtca tcaagatgtt gatgtgtgca gccatgctaa
     1561 atgcaattaa aataagtatc aatacaagcg gaataatcca tgggatatcc caaatattac
     1621 gtaatgaaac ggagccactt aaccaaaata ataggccttg taagtctgtt tcgttcataa
     1681 taagtatgcc ttgagtaaag gctgtaaata gcatcgcaat cgcagcacct gccaaaatga
     1741 cacggtgagg tgagaatagt gtttgtctaa acatacctag cgcaacaact aatacagtaa
     1801 caacaatggc acctaaaaat gcaataacta caatcatttt aaaagattga atttggataa
     1861 atgtaatact aaaaatgaca aaaaatactg cgcctgcatt gacaccgaaa agccctggtg
     1921 atgctattgg gtttcgtgta agtgcttgca tcaacaaacc tgagacagca agggcagcac
     1981 cagtcaataa cgcaatgatt gttctcgacg cccgtgcacc agtgacaaca tcatgtaaat
     2041 cgttttcact atcaaagttg aataacgcct gtatcaccgt acctggtgac acaagcgtat
     2101 ttccaatcat taaacttaag atagctacta ttgcaagaca taaaccagca ataacgattt
     2161 ggtattttgg tttaagtagc atcgtaaaac tccttaatta ttttgattgt ttttcaatat
     2221 ttaacttttc atataaatcg tcaatcaatt ttaatgatga tttatatccg ccagctaagt
     2281 tccaagtgat ttcatctaaa tcatcagata cttggttgtt tttaactgcg tctaaatttt
     2341 tccattcttt acttgaagtc cattcgcttt cagtcttttt aactaatgca gcatctttcg
     2401 catttggatc tgattttact acaaaaatat gatcagcgtt cattaatgga atgctttctt
     2461 tggatgtaag ttggataata tctttaccat tatcaacttg tttttgtaag tctttattac
     2521 gtttgaatcc taaatcattt aagatttcac cagcatatcc accagcataa attcttgtat
     2581 gatcagcacg gaagttaaca actgaagctt tcaatggcca tgcatcttta tactttgctt
     2641 ttgcatcttt ttggaatgca gctactttat catcgtactt tttaagtaaa tcttcagctt
     2701 ctttttcttt ccctaaagct ttccccatta acttagttgt atctttgaat ttgaaaactg
     2761 tatcagtaga aactgttggt gcgattttag ataattgatc gtaaactttt tcatttctaa
     2821 cttttgacgc gacaattaag tccggtttta atttagagat ttcctctaag ttaggtgcag
     2881 gttcttgacc tacaatctta gtatctttta aatcattttt tatgtattcg aatttcggtt
     2941 tttgtgtcca tgattctaca gcacctacag gtttaacacc taaagataca gcgacgtcag
     3001 tggcaccttg atatagcgta acaacacgct ttggtttccc tttaatttca gttgtaccca
     3061 ttgcatgttt aattgaagtt gtttccttat ctttgttatc agatgattgt ttatttgaat
     3121 tcccactaca tcctgctaaa acaagtagga aagcaagcgt aacaacaagc attttaatta
     3181 ctttattcat tgactaatta gcctccttcg tgatgtatga caatgagaat cattatcacg
     3241 atttagtatg aattaaattt tttcctaagt caataaaata tttatgattt acatgcaact
     3301 tataattatt tgacatataa atgcataaaa aatataatcc taattacttg atagtgagaa
     3361 tcattatcaa ttaggtaaca cacaatatta tagaatttta aatttgagga ggaagcgctt
     3421 ttgattgaaa aaagtcaagc atgtcacgat tcattgttag attctgtagg gcaaacacct
     3481 atggttcaac ttcatcaact atttccgaaa catgaagtgt ttgcaaagtt agagtatatg
     3541 aatcctggag gcagcatgaa agatcgacct gccaagtaca tcattgaaca tggtattaaa
     3601 catggtttaa tcactgagaa tacacattta attgaaagta cttctggtaa tttaggcatt
     3661 gcgttggcaa tgatagctaa aatcaaggga ttaaaactca cgtgtgttgt tgatcctaaa
     3721 atatcaccaa caaatttgaa aattattaaa agttatggtg ccaatgtaga aatggttgaa
     3781 gaacctgatg cacatggggg ttatttaatg actcgtattg caaaggtgca agaactgtta
     3841 gccactattg acgatgcata ttggattaat caatatgcga atgagttaaa ttggcaatcc
     3901 cattatcatg gtgcaggcac agagattgtt gaaacaatta agcaacctat agattatttt
     3961 gtcgcgccag tcagcacgac aggtagcatt atgggtatga gtagaaaaat aaaagaagtg
     4021 catccaaacg cacaaattgt tgctgttgat gcgaaagggt cagtcatttt tggtgacaaa
     4081 cctattaata gagaattacc tggtatcggt gctagtcgtg tacccgaaat attgaataga
     4141 tcagaaatta atcaagtgat ccatgtagat gattatcaat ctgctttggg ctgtcgaaaa
     4201 ctgattgatt atgaaggcat atttgccgga ggttcaacag gttcgattat tgcagcgatt
     4261 gagcagttga taacgtcaat tgaagaaggt gcaacaattg tcacgatttt accagatcga
     4321 ggcgatcgtt acttagattt agtttattca gatacatggt tagaaaaaat gaaatcaaga
     4381 caaggagtta aatcagaatg aatagagaga tgttgtattt aaatagatca gatattgaac
     4441 aagcgggagg taatcattca caagtttatg tggacgcatt aacagaagca ttaacagccc
     4501 atgcgcacaa tgattttgta caaccgctta agccgtattt aagacaggat cctgaaaatg
     4561 gacacatcgc agatcgaata attgcaatgc caagtcatat cggtggtgaa cacgcaattt
     4621 caggtattaa gtggataggt agtaagcacg acaatccatc gaaacgtaat atggagcgtg
     4681 caagtggtgt cattattttg aatgatccag aaacgaatta tccaattgca gttatggaag
     4741 caagtttaat tagtagtatg cgtactgcag cagtttcagt gattgcagct aagcatttgg
     4801 ctaaaaaagg atttaaagac ttaacaatca ttggatgcgg gctaatcgga gacaagcaat
     4861 tacaaagtat gttagagcaa ttcgatcata ttgaacgcgt gtttgtttac gatcaattct
     4921 ctgaagcatg tgcacgcttt gttgatagat ggcaacaaca gcgtccggaa attaatttta
     4981 ttgcgacaga aaatgctaaa gaagcagtat caaatggtga agtagtcatt acatgtaccg
     5041 taacggatca accatacatt gaatatgatt ggttacaaaa gggtgcattt attagcaaca
     5101 tttctatcat ggatgtgcat aaagaagtct ttattaaagc tgacaaagtc gtagtagacg
     5161 actggtcaca atgtaatcga gaaaagaaaa ctattaacca attggtgtta gaaggtaaat
     5221 tcagcaaaga agcacttcat gctgaactag gacaacttgt gacaggtgac ataccaggac
     5281 gtgaagacga tgatgaaatc atattactta atccgatggg tatggctatc gaagatattt
     5341 caagtgctta ctttatttat caacaggcac aacaacaaaa tattgggaca acattgaacc
     5401 tatattaaga atgcgaggtg tctgaacatt gcagaatcat acagcagtca atacagcaca
     5461 aacgataata ttaagagatt tagttgatgc attattattt gaagatatag ccggtattgt
     5521 atcgaatagt gatattacta aagaaaatgg acaaacgctt ttgatatacg aacgtgaaac
     5581 acaacaaata aagatacctg tttattttag tgctttaaat atgtttcgtt atgaaagctc
     5641 acaaccaatt acgatagagg gaagggcgtc taagcaacct ttaacagcag ctgaattttg
     5701 gcaaacaatt gctaatatga attgtgattt aagtcatgaa tgggaagtgg ctcgagttga
     5761 agaaggactg actactgctg ccacacagct tgctaaacaa ttatcagaat tagatttagc
     5821 gtcacatcct tttgtgatgt cagagcagtt tgcaagttta aaagatcgtc catttcatcc
     5881 attagctaaa gaaaaaagag gattaagaga agcggattat caagtgtatc aagctgaatt
     5941 aaatcaatca tttcctttaa tggttgcagc agttaaaaag acacatatga ttcatggcga
     6001 tactgcaaat atcgatgaat tagaaaattt gacagcacct ataaaagaac aagcgacaga
     6061 catgttaaat gatcaagggt tatcaataga tgactatgta ctatttccgg tacatccttg
     6121 gcaatatcag catattttgc cgaacgtctt tgcgaaagag attagtgaaa agttggttgt
     6181 actattaccg ttaaaatttg gagattatct gtcgtcttca agtatgcgtt cattaattga
     6241 tattggcgca ccgtataacc atgtcaaagt accatttgca atgcagtcat taggggcatt
     6301 aaggctaacg cctacgcgtt acatgaaaaa cggagaacaa gcagaacaat tattacgtca
     6361 gcttatagaa aaagatgaag cactagctaa gtatgtcatg gtttgtgatg aaacagcttg
     6421 gtggtcatat atgggtcaag ataatgatat tttcaaagat caattaggtc atctaactgt
     6481 tcagctaaga aaatatcccg aagtgctagc caaaaatgat acgcaacagc tagtgtcaat
     6541 ggcagcactc gcggcaaatg atcgcacttt atatcaaatg atttgtggaa aagataatat
     6601 ttctaaaaat gatgtcatga cgttatttga agatatcgcg caagtctttt taaaggtaac
     6661 actatcattt atgcaatacg gcgcattacc agagttgcat ggtcaaaata tattgttgtc
     6721 atttgaagat ggacgtgtac aaaaatgcgt gttacgtgat catgatactg tcagaattta
     6781 taaaccatgg ctaacagcac atcagctttc attgccgaag tatgtcgtca gagaagatac
     6841 acctaatacg ctaattaatg aagatttgga aacattcttt gcttattttc aaacattagc
     6901 tgtatcggta aatctatatg ccattattga tgcaattcaa gatttatttg gtgtaagtga
     6961 gcatgaactt atgtcgttgt taaaacaaat tttaaagaat gaagtggcaa ctatttcctg
     7021 ggttacaact gatcagctag ctgtcagaca cattttattt gataaacaga cgtggccatt
     7081 caaacaaatt ttattaccat tgctatatca acgtgatagt ggtggaggta gtatgccttc
     7141 aggtttaact accgtaccaa atccaatggt gacatatgat taatcagtct atatggcgca
     7201 gtaactttcg cattttatgg ctcagtcagt ttatagcgat tgctggactg acagtacttg
     7261 tgccattatt gccaatttat atggcatcac tacaaaatct atcagtcgta gaaatacagt
     7321 tgtggagtgg tatagcgatt gctgctccag ctgtaacgac gatgatagct tcgccgatat
     7381 gggggaagct aggtgataag attagccgga aatggatggt gttaagagcg ttacttggtt
     7441 tggcggtatg cttattttta atggcattgt gtacgacacc attacagttt gtacttgtga
     7501 ggttattgca gggactattt ggtggtgttg ttgatgcatc aagtgcgttt gcgagtgcag
     7561 aggcgccagc tgaagatcgt ggaaaggtat taggaagact gcaaagttca gtcagcgcag
     7621 ggtctcttgt ggggccatta attggcggtg ttacagcttc gatattaggt tttagtgcgt
     7681 tactgatgag tattgccgtt attactttta ttgtctgtat tttcggtgca ttaaaattga
     7741 ttgaaacgac acatatgcca aaatcacaaa caccaaatat taataaaggt attcgccgtt
     7801 catttcaatg tctattatgc acacaacaaa catgtcgatt tattatcgtt ggcgttttag
     7861 caaactttgc tatgtatggc atgctaactg cattatcacc acttgcttca tcagtgaatc
     7921 atacagcgat agatgaccgt agtgtgattg gatttttaca gtccgcattt tggacggctt
     7981 cgatattaag cgcgccttta tggggacgct ttaatgataa atcatatgtt aaatcagtat
     8041 atatatttgc cacgattgca tgtggttgta gtgcgatact gcaaggttta gcgacgaata
     8101 tagagttttt aatggctgca agaatacttc aaggattaac atatagtgca ttgattcaaa
     8161 gtgtcatgtt tgttgtcgtg aatgcgtgtc atcaacaact taaaggcaca tttgttggaa
     8221 cgacgaacag tatgttagtt gttggtcaaa ttattggcag tcttagtggc gctgccatta
     8281 caagttatac tacaccagct actacgttta tcgttatggg cgtagtattt gcagtaagta
     8341 gtttattttt aatttgttca accatcacta atcaaatcaa cgatcacaca ttaatgaaat
     8401 tatgggagtt gaaacaaaaa agtgcaaaat aaagaattaa tacaacatgc agcgtatgcg
     8461 gctatcgaac gcattttaaa tgaatatttt agagaagaaa atttatatca agtaccacct
     8521 caaaatcatc aatggtctat acaattatca gagctcgaaa ctttaacggg tgaatttcgc
     8581 tattggtctg cgatggggca tcatatgtat catccagagg tatggcttat cgatggaaaa
     8641 agtaaaaaaa taacaactta taaagaagca attgcgcgta ttttgcaaca tatggctcaa
     8701 agtgcagata atcaaacggc agtgcaacaa catatggcgc aaattatgtc tgacatcgat
     8761 aatagcattc atcgcacggc gcgttatttg caaagtaaca caatagacta cgtagaggat
     8821 cgttatatcg tttcagaaca atctttatac ttaggtcatc catttcatcc gactcctaag
     8881 agtgcaagtg ggttttcaga agcagattta gagaaatatg cacccgaatg tcatacatca
     8941 ttccaattgc attatttagc tgtgcatcaa gatgttctgc tcacgcgcta tgtagaaggt
     9001 aaagaagatc aggttgagaa agtgttgtat caattagcag acatagatat atcagagata
     9061 cccaaaaatt ttattttatt accaacacat ccttatcaaa tcaatgtgtt gcgacagcat
     9121 ccacagtata tgcaatatag tgaacaaggt ttaataaaag accttggcgt ttccggtgat
     9181 tcagtgtacc cgacgtcttc ggttagaact gtattttcaa aagcattaaa catttattta
     9241 aaattaccga tacacgttaa aatcactaat tttatacgta cgaatgacct tgaacagatt
     9301 gaacggacaa ttgatgccgc gcaagttatc gcatcagtca aagatgaggt tgaaacaccc
     9361 cattttaaat tgatgtttga agaaggatat cgtgcattgt taccgaatcc attagggcaa
     9421 acagttgaac ctgaaatgga tttattaaca aatagtgcca tgattgttcg tgaagggata
     9481 ccgaattacc atgctgataa agatattcat gtattggcgt cattatttga aacgatgcct
     9541 gattcaccga tgtctaagtt atcacaagtg attgagcaaa gtggtttagc accagaagca
     9601 tggcttgaat gttatttgaa tcgtacatta ttgccgatat taaagctgtt tagtaacaca
     9661 ggcattagtc tagaagcaca tgtacaaaat acattaattg aattaaaaga tggcataccc
     9721 gacgtatgct ttgtcagaga tcttgaaggc atttgtctat ctagaacgat tgctactgaa
     9781 aaacaacttg tgccaaatgt tgtggcagca tcaagccctg ttgtatatgc acatgatgaa
     9841 gcatggcatc gtcttaaata ttacgttgta gtaaatcact taggacattt agtatcaact
     9901 attggtaaag cgactagaaa tgaagttgtg ttatggcaac ttgtagcgca tcgtcttatg
     9961 acttggaaaa aagaatacgc gaataacgca gtgtttgttg actgtgtaga agatttatat
    10021 caaacgccga ccattgcggc taaagcgaat ttgatgagta aattgaatga ttgtggtgca
    10081 aaccctattt atacacatat accaaatcca atttgtcata acaaggaggt atcgtattgt
    10141 gaatcaaaca attcttaatc gtgtaaagac tagagtgatg caccaactgg tatcatcact
    10201 tatttatgag aatattgttg tgtataaagc gtcatatcaa gacggtgccg gtcattttac
    10261 aatagaagga catgattcag agtatcgttt tactgctgaa aagacacata gctttgatcg
    10321 tatacgtatc acatcaccaa ttgagcgtgt cgtaggagat gaggcagata caacaacaga
    10381 ctatacacaa ttattgagag aggttgtatt tacatttcct aaaaatgatg aaaagctaga
    10441 acaatttatt gttgagttat tacagacaga attaaaagat acacaaagta tgcagtatcg
    10501 agaatcaaac ccaccagcaa cacctgagac atttaacgac tatgaatttt atgcgatgga
    10561 agggcatcag tatcatccaa gttacaaatc acgtttagga tttacgttga gtgataattt
    10621 gaaatttggt cctgattttg taccaaacgt taaactgcag tggttagcta tcgacaaaga
    10681 taaagtagaa acgacggtat caagaaatgt tgtagttaac gaaatgttac gtcaacaagt
    10741 tggcgataag acttatgaac attttgtaca gcaaattgaa gcgtctggca aacatgtaaa
    10801 tgatgttgag atgatacctg tacacccatg gcagtttgaa catgtcatcc aagttgattt
    10861 ggctgaagaa aggcttaatg gcacagtact atggttaggg gaaagtgatg agctatatca
    10921 tcctcaacaa tcgattcgta cgatgtcgcc aatagacacg acaaaatatt atttaaaggt
    10981 accaataagt ataacgaaca cttcaacgaa acgagtgttg gcgcctcata caattgaaaa
    11041 tgcagcgcaa attacggatt ggttaaagca gatacagcaa caagatacgt atttaaaaga
    11101 tgaattaaag acagcttttc taggggaagt cttaggacag tcttatttaa atacacaact
    11161 ttcgccttat aaacaaactc aagtttatgg tgcgttaggt gttatatggc gtgaaaatat
    11221 atatcacatg ttaatcgatg aagaggatgc gataccattt aatgcacttt atgcaagtga
    11281 taaggatggt gtaccattca ttgaaaattg gattaaacaa tatggttctg aagcttggac
    11341 aaagcaattt ttagctgtag cgattcgtcc aatgattcat atgctttatt atcacggtat
    11401 tgcctttgaa tcgcatgcac aaaatatgat gctcattcat gaaaatggtt ggcctacacg
    11461 tattgcctta aaagatttcc atgatggtgt tcgttttaag cgtgagcatt taagtgaagc
    11521 tgcttcacac ctgacattaa agccaatgcc agaagcacat aaaaaagtga atagtaattc
    11581 atttattgaa acagatgacg aacgtttagt acgcgacttt ttacatgatg catttttctt
    11641 tattaatatc gccgaaatca tcttatttat tgaaaagcaa tatggtatcg atgagcagcg
    11701 acaatggcaa tgggttaaag acattatcga ggcgtatcaa gaagcatttc cagagttgaa
    11761 taactatcaa catttcgatt tgtttgaacc tacgattcaa gttgaaaagt taacgacacg
    11821 tcgattatta agtgactccg agttaagaat tcatcatgtt acaaatccat taggtgtagg
    11881 aggtatcaat gatgcaacaa ctatctctga aacatagatt aaacaatggt gattcagttt
    11941 atggcatttt taattctata ccggacccat tgatgatcga ggttatcgca gcaagcgggt
    12001 atgactttgt tgtgattgat acagaacacg tggcgattaa tgatgagaca ctagcgcatt
    12061 taattcgtgc agctgaagca gcgcatatta taccaattgt acgtgtcact gcagtgatag
    12121 atagagatat cattaaagtg ttagatatgg gtgcgagagg tattattgtg ccacacgtta
    12181 aagatcgtga gacagttgag catattgtga aattaagtcg ttattacccg caaggattaa
    12241 gaagtttgaa tggtggtcgc atggcaagat ttggacgtac accattactt gatgcaatgg
    12301 agatggctaa tgagcatatt atggtgattg ccatgataga agatgttgaa ggggttatgg
    12361 ccattgacga tatagcacaa gtcgaaggtt tagacatgat agtcgaaggt gccgcagatt
    12421 tatcgcagtc acttggcata ccatggcaaa cgcgtgatga tcaagtaaca tcacatgttc
    12481 aacatatttt tgaggttgtg aatgcacatg gtaaacattt ttgtgcatta ccacgtgaag
    12541 atgaagatat tgcaaaatgg caggcacaag gtgtacaaac atttatttta ggtgatgatc
    12601 gcggaaaaat atatcgccat ttaagtgcat ctctagcgac gtctaaacag aaaggggatg
    12661 atggctaatg cgtatagttc aacctgttat tgaacaatta aaagcacaat ctcatccagt
    12721 ttgtcattat atctatgatt tagtcggact ggaacatcat ttgcaacata ttacattgtc
    12781 attgccgagt aattgtcaaa tgtactatgc aatgaaagca aatagtgaac gaacaatcct
    12841 agatacaatt agtcagtatg ttgaaggatt cgaagttgca tctcaaggtg aaatagcaaa
    12901 aggtcttgct tttaaaccag caaatcatat tatttttgga ggccctggta agacagacga
    12961 ggaactaaga tatgcagtaa gtgaaggtgt tcagcgtatt catgttgaaa gtatgcatga
    13021 attacaacgg ctaaatgcca tcttagaaga tgaagataag acacaacaca ttttattgcg
    13081 tgttaattta gcaggaccat ttcccaatgc aacgttgcat atggcaggac gcccaacaca
    13141 atttggtatt tctgaagacg aagttgatga tgtcattgaa gctgcgctag caatgccaaa
    13201 tattcatcta gatggctttc attttcattc tatttctaac aatttagact cgaatttaca
    13261 cgtcgatgta gtgaaacttt attttaaaaa agcaaaatca tggtctgaaa aacatcgatt
    13321 tccactcaaa catatcaatc ttggtggtgg tataggggtt aactatgcag atttaactag
    13381 ccaatttgag tgggataatt ttgtagaaaa ttttaaaaca cttatcgttg agcaagaaat
    13441 ggaagatgtg acattgaatt ttgaatgtgg gcgctttatt gtggcacata ttggttacta
    13501 tgtgacagaa gtgctagata ttaagaaagt gcatggtgct tggtatgcca ttttaagagg
    13561 aggtacgcaa caatttagac tgccggtatc ttggcagcat aaccatcctt ttgaaattta
    13621 tcgctataag gacaatccat attcatttga aaaagtttca atttcgagac aggacacaac
    13681 gttagtcggt caattatgta caccgaaaga tgtctttgct agagaagtac agatagacgc
    13741 aatcagtaca ggcgacgtta ttgttttcaa atatgcaggt gcatacggat ggtctatttc
    13801 acatcacgat ttcttaagcc atccacatcc tgaatttatt tatttaacgc aaacaaagga
    13861 ggatgaataa ctattgaatc atattcatga acatttaaaa ttggtaccag tagataagat
    13921 tgatcttcac gaaacattcg aacctttaag attggaaaag acgaaaagta gtattgaagc
    13981 agatgatttt atacgtcatc ctattttagt gacagcgatg caacatggta gatatatggt
    14041 tatagatggt gtgcatcggt atacaagttt gaaagcgtta ggatgtaaga aagttccagt
    14101 gcaagaaatc catgaaacac aatattcaat tagtacgtgg caacataaag ttccatttgg
    14161 tgtgtggtgg gaaacgttac aacaagaaca tcgcttgcca tggactactg agacaagaca
    14221 agaagcgcca tttattacga tgtgtcatgg tgatacagaa caatatttgt atacgaaaga
    14281 tttaggcgaa gcacattttc aagtatggga aaaggttgtc gcaagttata gtggttgttg
    14341 ttctgtagag agaattgcac aaggtacata tccttgtctt tctcaacaag atgtactcat
    14401 gaagtatcag ccattgagtt ataaggaaat tgaagcggtt gttcataaag gggaaactgt
    14461 gccagcaggt gtgacacgct ttaatatttc aggacgatgt cttaatcttc aagtaccact
    14521 ggcattactt aaacaagatg atgatgttga acaactgcgc aattggaagc agtttttagc
    14581 agataagttt gccaatatga gatgctatac tgaaaaagta tacttggtgg agcaatagtt
    14641 ttactgtgat gttgagggaa atatgatgat ttagcgtatt gatagcgaaa atataataaa
    14701 acaatatagt gtggagaact tttgatattt tataaatatt gaagttctcc atttttgtat
    14761 tttgcatata aaaattaaat aaaataaggt atattaaggt aaagtataaa ttttaaataa
    14821 atggggaatg agtatgagct caattatagg aaaaatagca atttggatag gcatcgtagc
    14881 tcaaatatat tttagtgtcg tttttgttag gatgatatct attaatattg ctggaggatc
    14941 tgattacgaa acaatttttt tattaggatt aatattggct cttttcactg ttttaccaac
    15001 catctttact gcgatttata tggaaagtta ctctgtaatc ggaggtgcac tttttattgt
    15061 ttatgctatt attgcactgt gtttatataa tttcttttcg tcaattttat ggctgattgg
    15121 tggtattttg ctgatttgga ataaatactc aaaagatgaa tcgacagacg aaaatgaaaa
    15181 agttgatatt gaaagtacag agaatcaatt tgaatctaaa gataaaatca ctaaagaata
    15241 aagagaatat ttaaggcgga gtataaatat taaataaatg gggaatagac atggaaaaaa
    15301 atgt
//
LOCUS       DAMZKC010000052        15133 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.052, whole genome shotgun sequence.
ACCESSION   DAMZKC010000052 DAMZKC010000000
VERSION     DAMZKC010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 15133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15133
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.052"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            81..374
                     /gene="esxA"
                     /locus_tag="POB55_001220"
     CDS             81..374
                     /gene="esxA"
                     /locus_tag="POB55_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion effector EsxA"
                     /protein_id="HDI6500191.1"
                     /translation="MAMIKMSPEEIRAKSQSYGQGSDQIRQILSDLTRAQGEIAANWE
                     GQAFSRFEEQFQQLSPKVEKFAQLLEEIKQQLNSTADAVQEQDQQLSNNFGLQ"
     gene            457..3486
                     /gene="esaA"
                     /locus_tag="POB55_001221"
     CDS             457..3486
                     /gene="esaA"
                     /locus_tag="POB55_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EsaA"
                     /protein_id="HDI6500192.1"
                     /translation="MKKKNWIYALIVTLIIIIAIVSMIFFVQTKYGDQSEKGSRSVSN
                     KNNKIHIAIVNEDQPTTYNGKKVELGQAFIKRLANEKNYKFETVTRNVAESGLKNGGY
                     QVMIVIPENFSKLAMQLDAKTPSKISLQYKTAVGQKEEVAKNTEKVVSNVLNDFNKNL
                     VEIYLTSIIDNLHNAQKNVGAIMTREHGVNSKFSNYLLNPINDFPELFTDTLVNSISA
                     NKDITKWFQTYNKSLLSANSDTFRVNTDYNVSTLIEKQNSLFDEHNTAMDKMLQDYKS
                     QKDSVELDNYINALKQMDSQIDQQSSMQDTGKEEYKQTVKENLDKLREIIQSQESPFS
                     KGMIEDYRKQLTESLQDELANNKDLQDALNSIKMNNAQFAENLEKQLHDDIVKEPDTD
                     TTFIYNMSKQDFIAAGLNEDEANKYEAIVKEAKRYKNEYNLKKPLAEHINLTDYDNQV
                     AQDTSSLINDGVKVQRTETIKSNDINQLTVATDPHFNFEGDIKINGKKYDIKDQSVQL
                     DTSNKEYKVEVNGVAKLKKDAEKDFLKDKTMHLQLLFGQANRQDEPNDKKTTSVVDVT
                     LNHNLDGRLSKDALSQQLSALSRFDAHYKMYTDTKGREDKPFDNKRLIDMMVDQVIND
                     MESFKDDKVAVLHQIDSMEENSDKLIDDILNNKKNTTKNKEDISKLIDQLENVKKTFA
                     EEPQEPKIDKGKNDEFNTMSSNLDKEISRISEKSTQLLSDTQESKTIADSVSGQLNQL
                     DNNVNKLHATGRALGVRANDLNRQMAKNDKDNELFAKEFKKVLQNSKDGDRQNQALKA
                     FMSNPVQKKNLENVLANNGNTDVISPTLFVLLMYLLSMITAYIFYSYERAKGQMNFIK
                     DDYSSKNHLWNNVITSGVIGTTGLVEGLIVGLIAMNKFHVLAGYRAKFILMVILTMMV
                     FVLINTYLLRQVKSIGMFLMIAALGLYFVAMNNLKAAGQGVTNKISPLSYIDNMFFNY
                     LNAEHPIGLALVILTVLVIIGFVLNMFIKHFKKERLI"
     gene            3486..3944
                     /gene="essA"
                     /locus_tag="POB55_001222"
     CDS             3486..3944
                     /gene="essA"
                     /locus_tag="POB55_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssA"
                     /protein_id="HDI6500193.1"
                     /translation="MLMNSVIALTFLTASSNNGGLNIDVQQEEEKRINNDLNQYDTTL
                     FNKDSKAVNDAIAKQKKERQQQIKNDMFQNQASHSTRLNETKKVLFSKSNLEKTSESD
                     KSPYIQNKQEKKIFPYILMSVGAFLTLGFVIFSIHKGRRTKNESARKSNI"
     gene            3916..4158
                     /gene="esaB"
                     /locus_tag="POB55_001223"
     CDS             3916..4158
                     /gene="esaB"
                     /locus_tag="POB55_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EsaB"
                     /protein_id="HDI6500194.1"
                     /translation="MNQHVKVTFDFTNYNYGTYDLAVPAYLPIKNLIALVLDSLDISI
                     FDVNTQIKVMTKGQLLVENDRLIDYQIADGDILKLL"
     gene            4171..5505
                     /gene="essB"
                     /locus_tag="POB55_001224"
     CDS             4171..5505
                     /gene="essB"
                     /locus_tag="POB55_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssB"
                     /protein_id="HDI6500195.1"
                     /translation="MVKNHNPKNEMQDMLTPLDAEEAAKTKLRLDMREIPKSSIKPEH
                     FHLMYLLEQHSPYFIDAELTELRDSFQIHYDINDNHTPFDNIKSFTKNEKLRYLLNIK
                     NLEEVNRTRYTFVLAPDELFFTRDGLPIAKTRGLQNVVDPLPVSEAEFLTRYKALVIC
                     AFNEKQSFDALVEGNLELHKGTPFETKVIEAATLDLLTAFLDEQYQKQEQDYSQNYAY
                     VRKVGHTVFKWVAIGMTTLSVLLIAFLAFLYFSVMKHNERIEKGYQAFVKDDYTQVLN
                     TYDDLDGKKLDKEALYIYAKSYIQTNKQGLEKDKKENLLNNVTPNSNKDYLLYWMELG
                     QGHLDEAINIATYLDDNDITKLALINKLNEIKNNGDLSNDKRSEETKKYNDKLQDILD
                     KEKQVKDEKAKSEEEKAKAKDEKLKQQEENEKKQKEQAQKDKEKRQEAERKK"
     gene            5527..9966
                     /gene="essC"
                     /locus_tag="POB55_001225"
     CDS             5527..9966
                     /gene="essC"
                     /locus_tag="POB55_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssC"
                     /protein_id="HDI6500196.1"
                     /translation="MHKLIIKYNKQLKMLNLRDGKTYTISEDERADITLKSLGEVIHL
                     EQNNQGTWQANHTSINKVLVRKGDLDDITLQLYTEADYASFAYPSIQDTMTIGPNAYD
                     DMVIQSLMNAIIIKDFQSIQESQYVRIVHDKNTDVYINYELQEQLTNKAYIGDHIYVE
                     GIWLEVQADGLNVLSQNTVASSLIRLTQEMPHAQADDYNTYHRSPRIIHREPTDDIKI
                     ERPPQPIQKNNTVIWRSIIPPLVMIALTVVIFLVRPIGIYILMMIGMSSVTIVFGITT
                     YFSEKKKYNKDVEKREKDYKDYLDNKSKEINKAIKAQRFSLNYHYPTVAEIKDIVETK
                     APRIYEKTSHHHDFLHYKLGIANVEKSFKLDYQEEEFNQRRDELFDDAKELYEFYTDV
                     EQAPLINDLNHGPIAYIGARHLILEELEKMLIQLSIFHSYHDLEFLFVTREDEVETLK
                     WARWLPHMTLRGQNIRGFVYNQRTRDQILTSIYSMIKERIQAVRERSKSNEQIIFTPQ
                     LVFVITDMSLIIDHVILEYVNQDLSEYGISLIFVEDVIESLPEHVDTIIDIKSRTEGE
                     LITKEKELVQLKFTPENIDNVDKEYIARRLANLIHVEHLKNAIPDSITFLEMYNVKEV
                     DQLDVVNRWRQNETYKTMAVPLGVRGKDDILSLNLHEKAHGPHGLVAGTTGSGKSEII
                     QSYILSLAINFHPHEVAFLLIDYKGGGMANLFKDLVHLVGTITNLDGDEAMRALTSIK
                     AELRKRQRLFGEHDVNHINQYHKLFKEGIATEPMPHLFIISDEFAELKSEQPDFMKEL
                     VSTARIGRSLGIHLILATQKPSGVVDDQIWSNSKFKLALKVQDRQDSNEILKTPDAAD
                     ITLPGRAYLQVGNNEIYELFQSAWSGATYDIEGDKLEVEDKTIYMINDYGQLQAINKD
                     LSGLEDEETKENQTELEAVIDHIESITTRLEIEEVKRPWLPPLPENVYQEDLVETDFR
                     KLWSDDAKEVELTLGLKDVPEEQYQGPMVLQLKKAGHIALIGSPGYGRTTFLHNIIFD
                     VARHHRPDQAHMYLFDFGTNGLMPVTDIPHVADYFTVDQEDKIAKAIRIFNDEIDRRK
                     KILSQYRVTSISEYRKLTGETIPHVFILIDNFDAVKDSPFQEVFENMMIKMTREGLAL
                     DMQVTLTASRANAMKTPMYINMKTRIAMFLYDKSEVSNVVGQQKFAVKDVVGRALLSS
                     DDNVSFHIGQPFKHDETKSYNDQINDEVSAMTEFYKGETPSDIPMMPDEIKYEDYRES
                     LSLPDIVANGALPIGLDYEGVTLQKIKLTEPAMISSENPREIAHIAEIMMKEIDILNE
                     KYAICIADSSGEFKAYRHQVANFAEEREDIKAIHQLMIEDLKQREMDGPFEKDSLYII
                     NDFKTFIDCTYIPEDDVKKLITKGPELGLNILFVGIHKELIDAYDKQIDVARKMINQF
                     SIGIRISDQQFFKFRFIQREPVIKENEAYMVANQAYQKIRWFK"
     gene            9996..10388
                     /gene="esaC"
                     /locus_tag="POB55_001226"
     CDS             9996..10388
                     /gene="esaC"
                     /locus_tag="POB55_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion substrate EsaC"
                     /protein_id="HDI6500197.1"
                     /translation="MNFNDIETMVKSKFKDIKKHAEEIAHEIEVRSGYLRKAEQYKRL
                     EFNLSFALDDIESTAKDVQTAKTSANKDSVTVKGKAPNTLYIEKRNLMKQKLEMLGED
                     IDKNKESLQKAKEIAGEKASEYFNKAMN"
     gene            10404..10718
                     /gene="esxB"
                     /locus_tag="POB55_001227"
     CDS             10404..10718
                     /gene="esxB"
                     /locus_tag="POB55_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion effector EsxB"
                     /protein_id="HDI6500198.1"
                     /translation="MGGYKGIKADGGKVDQAKQLAAKTAKDIEACQKQTQQLAEYIEG
                     SDWEGQFANKVKDVLLIMAKFQEELVQPIADHQKAIDNLSQNLAKYDTLSIKQGLDRV
                     NP"
     gene            10718..11392
                     /locus_tag="POB55_001228"
     CDS             10718..11392
                     /locus_tag="POB55_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5081 family protein"
                     /protein_id="HDI6500199.1"
                     /translation="MKDVKRIDYFSYEELTILGGSKLPLVNFELFDPSNFEEAKAALI
                     EKELVTENDKLTDAGFKVATLVREYISAIVNIRINDMYFAPFSYEKDEYILLSRFKNN
                     GFQIRIINKDIAWWSIVQSYPLLMRQEKSNDWDFKQIDDETLENLNNESIDTIGRVLE
                     IEIYNHQGDPQQSLYNIYEQNDLLLIRYPLKDKVLNVHIGVINTFIRELFGFNTDENH
                     INKAEE"
     gene            11392..11709
                     /locus_tag="POB55_001229"
     CDS             11392..11709
                     /locus_tag="POB55_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500200.1"
                     /translation="MTLSGKISVKAETIAHVVKELESISQKYDEIAQNFGKIAQLNYY
                     SSEKASHSMENGYSSAATVISGLKGPLSTLGGGVMNSAQKFFEADEHWGTEFAKLYYN
                     IEG"
     gene            11719..13563
                     /locus_tag="POB55_001230"
     CDS             11719..13563
                     /locus_tag="POB55_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease YeeF family protein"
                     /protein_id="HDI6500201.1"
                     /translation="MTKDIEYLTADYDNEKSSIQSVIDAIEGQDFLDVDTTMDDAVSD
                     VSSLDEDGAISLTSSVVGPQGSKLMGYYQNELYDYASQLDSKMKEIIDTPFIEDIDKA
                     FKGITNVKLENILIKNGGGHGRDTYGASGKIAKGDAKKSDSDVYSIDEILKSDQEFVK
                     VIDQHYKEMKKEDKKLSKSDFEKMMTQGASCDYMTVAEAEELEEQKKKEEAIEIAALA
                     GMVVLSCINPVAGAVAIGAYSAYSAANAATGKNIVTGRKLSKEERIMEGLSLIPLPGM
                     GFLKGAGKSLMKLGFKGGEKFAVKTGLQKTMQQAVSRISPKMGMMKNSVLNQSRNFAQ
                     NTHVGQMLSNMRGQATHTVQQSRNWIGQQAQNVKRIVNNGLDKEIAHPFKQQLAPAGM
                     GGIKFAETTTLRNMGQNMKRAVTPQNHVTHGPKDSMVRSEGKHSVSSHEINSSKYVES
                     PNYTKVEFGEHYARLRPKKLKANIEYTTPTGHIYRTDHKGRIKEVYVDNLSLKDGGRN
                     NHAQRTVGGEDRLPDDDGGHLIARMFGGSKDIDNLVAQSKFINRSFKENGQWYKLEKL
                     WEKAIKSGKSIENVKVEVKYKGNSQRPTEFSVRYNINGEQFLKNIINK"
     gene            13574..14068
                     /locus_tag="POB55_001231"
     CDS             13574..14068
                     /locus_tag="POB55_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HDI6500202.1"
                     /translation="MTFEEKLSQMYNEIANKISSMIPVEWEKVYAMAYIDDEGGEVFY
                     YYTEPGSNELYYYTSVLNKYDISESEFMDSAYELYKQFQKLRNIFKKEGHEPWTSCEF
                     DFTNEGKLKVSFDYIDWINTEFDQLGRQNYYMYKKFGVIPEMEYEMEEVKQIEQYIKE
                     QEEE"
     gene            14279..14961
                     /locus_tag="POB55_001232"
                     /pseudo
     CDS             14279..14961
                     /locus_tag="POB55_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498863.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5079 family protein"
BASE COUNT         5906 a         2050 c         2828 g         4349 t
ORIGIN      
        1 ttgagtaagg agcacttttt caaaaaatag tgtccctaaa aagttttgat aaacttaaaa
       61 tattcaggag gtttctagtt atggcaatga ttaagatgag tccagaggaa atcagagcaa
      121 aatcgcaatc ttacgggcaa ggttcagacc aaatccgtca aattttatct gatttaacac
      181 gtgcacaagg tgaaattgca gcgaactggg aaggtcaagc tttcagccgt ttcgaagagc
      241 aattccaaca acttagtcct aaagtagaaa aatttgcaca attattagaa gaaattaaac
      301 aacaattgaa tagcactgct gatgccgttc aagaacaaga ccaacaactt tctaataatt
      361 tcggtttgca ataagcattc tgaaattggc aaagtcacat tttctaatgt ggctttgctt
      421 atcatttttt aagaaaacaa ctgaaaggaa ataagcatga aaaagaaaaa ttggatttat
      481 gcattaattg tcactttaat tattataatt gccatagtta gtatgatatt ttttgttcaa
      541 acaaaatatg gagatcaatc agaaaaagga tcccgaagtg taagtaataa aaataataaa
      601 atacatatcg caattgttaa cgaggatcaa ccaacgacat ataacggtaa aaaagttgag
      661 ctgggtcaag catttattaa aaggttagca aatgagaaaa actataaatt tgaaacagta
      721 acacgaaacg ttgctgagtc tggtttgaaa aatggtggat accaagtcat gattgttatc
      781 ccagaaaact tttcaaaatt ggcaatgcaa ttagacgcta aaacaccatc gaaaatatcg
      841 ctacagtata aaacagctgt aggacaaaaa gaagaagtag ctaaaaacac agaaaaagtt
      901 gtaagtaatg tacttaacga ctttaacaaa aacttagtcg aaatttattt aacaagtatc
      961 attgataatt tacataatgc acaaaaaaat gttggcgcta ttatgacgcg tgaacatggt
     1021 gtgaatagta aattctcgaa ttacttatta aatccaatta acgacttccc ggaattattt
     1081 acagatacgc ttgtaaattc aatttctgca aacaaagata ttacaaaatg gttccaaaca
     1141 tacaataaat cattattgag tgcgaattca gatacgttca gagtgaacac agattataat
     1201 gtttcgactt taattgaaaa acaaaattca ttatttgacg agcacaatac agcgatggat
     1261 aaaatgttac aagattataa atcgcaaaaa gatagcgtgg aacttgataa ctatatcaat
     1321 gcattaaaac agatggacag ccaaattgat caacaatcaa gtatgcaaga tacaggtaaa
     1381 gaagaatata aacaaactgt taaagaaaac ttagataaat taagagaaat cattcaatca
     1441 caagagtcac cattttcaaa aggtatgatt gaagactatc gtaagcaatt aacagaatcg
     1501 ctgcaagatg agcttgcaaa taacaaagac ttacaagatg cgctaaatag cattaaaatg
     1561 aacaatgctc aattcgctga aaacttagag aaacaacttc atgatgatat tgtcaaagaa
     1621 cctgatacag atacaacatt tatctataac atgtctaaac aagactttat agctgcaggt
     1681 ttaaatgagg atgaagctaa taaatacgaa gcaattgtca aagaagcaaa acgttataaa
     1741 aacgaatata atttgaaaaa accgttagca gaacacatta atttaacaga ttacgataac
     1801 caagttgcgc aagacacaag tagtttgatt aatgatggtg tgaaagtgca acgtactgaa
     1861 acgattaaaa gtaatgatat taatcaatta actgttgcaa cagatcctca ttttaatttt
     1921 gaaggcgaca ttaaaattaa tggtaaaaaa tatgacatta aggatcaaag tgttcaactc
     1981 gatacatcta acaaggaata taaagttgaa gtcaatggcg ttgctaaatt gaaaaaggat
     2041 gctgagaaag atttcttaaa agataaaaca atgcatttac aattgttatt tggacaagca
     2101 aatcgtcaag atgaaccaaa tgataagaaa acaacgagtg ttgtggatgt aacattgaat
     2161 cataaccttg atggtcgctt atcgaaagat gcattaagcc agcaattgag tgcattatct
     2221 aggtttgatg cgcattataa aatgtacaca gatacaaaag gcagagaaga taaaccattc
     2281 gacaacaaac gtttaattga tatgatggtt gaccaagtta tcaatgacat ggaaagtttc
     2341 aaagacgata aagtagctgt gttacatcaa attgattcaa tggaagaaaa ctcagacaaa
     2401 ctgattgatg acattttaaa taacaaaaag aatacaacaa aaaataaaga agatatttct
     2461 aagctgattg atcagttaga aaacgttaaa aagacttttg ctgaagagcc acaagaacca
     2521 aaaattgata aaggcaaaaa tgatgaattt aatacgatgt cttcaaattt agataaagaa
     2581 attagtagaa tttctgagaa aagtacgcaa ttgctatcag atacacaaga atcaaaaaca
     2641 attgcagatt cagttagtgg acaattaaat caattagata ataatgtgaa taaactacat
     2701 gcgacaggtc gagcattagg cgtaagagcg aatgatttga accgtcaaat ggctaaaaac
     2761 gataaagata atgagttatt cgctaaagag tttaaaaaag tattacaaaa ttctaaagat
     2821 ggcgacagac aaaaccaagc attaaaagca tttatgagta atccggttca aaagaaaaac
     2881 ttagaaaatg ttttagctaa taatggtaat acagacgtga tttcaccgac attattcgta
     2941 ttattgatgt atttactatc aatgattaca gcatatattt tctatagtta tgaacgtgcc
     3001 aaaggacaaa tgaatttcat taaagatgat tatagtagta aaaaccatct ttggaataat
     3061 gtcattacgt caggtgttat tggtacaact ggtttggtag aaggattaat tgtcggttta
     3121 attgcaatga ataagttcca tgtattagct ggctatagag cgaaattcat cttaatggtg
     3181 attttaacta tgatggtctt cgtacttatt aatacgtatt tactaagaca ggtaaaatct
     3241 atcggtatgt tcttaatgat tgctgcattg ggtctatact ttgtagctat gaataatttg
     3301 aaagcggctg gacaaggtgt gactaataaa atttcaccat tgtcttatat cgataacatg
     3361 ttcttcaatt atttaaatgc agagcatcct ataggcttgg cgctagtaat attaacagta
     3421 cttgtgatta ttggctttgt actgaacatg tttataaaac actttaagaa agagagatta
     3481 atctaatgtt gatgaatagc gtgattgctt taactttttt aacagcatct agcaataatg
     3541 gcggacttaa tattgacgtg caacaagaag aggaaaagcg aatcaataat gatttaaatc
     3601 aatatgatac aacgctattt aataaagaca gcaaagcggt taatgatgcg attgctaagc
     3661 agaaaaaaga acgacaacaa caaataaaaa atgatatgtt tcaaaatcaa gcgagtcact
     3721 cgactcgctt gaatgaaact aaaaaagtgt tattttccaa atctaactta gaaaagactt
     3781 cggagagtga taaaagcccc tatattcaaa acaagcagga gaaaaaaata ttcccgtaca
     3841 ttttgatgtc tgtaggggct tttttgactt taggatttgt cattttttca attcataaag
     3901 ggagacgaac gaaaaatgaa tcagcacgta aaagtaacat ttgattttac taattataat
     3961 tacggcacat atgacttagc agtaccagca tatttaccga taaaaaactt aatagcttta
     4021 gtattggata gtttggacat ttcaatattt gatgtcaata cacaaattaa agtgatgacg
     4081 aaaggtcaat tacttgttga aaatgatcga ctcattgatt atcaaatcgc tgatggagat
     4141 attttgaagt tactatagga ggaaaaatag atggttaaaa atcataaccc taaaaatgaa
     4201 atgcaagata tgttaacgcc tttagatgct gaagaagcag ctaaaacaaa attacgctta
     4261 gatatgagag agattcctaa gtcttcaatt aaaccagaac attttcattt aatgtactta
     4321 ttagaacaac attctccata ttttatagat gctgaattaa ctgaactacg tgacagtttc
     4381 caaatacatt atgacattaa tgacaatcat acaccttttg ataatattaa atcatttact
     4441 aaaaatgaaa aattacgtta cttactcaat atcaaaaatt tagaagaagt aaatcgtaca
     4501 cgctacacat ttgtgttggc accagatgaa ttatttttca caagagatgg attacccatt
     4561 gctaaaacaa gagggttaca aaatgttgtt gatccattac ctgtgtcaga agctgaattt
     4621 ttaacaagat ataaagcact ggttatctgt gcattcaatg agaaacaatc atttgatgct
     4681 ttagttgaag gaaacttaga actacataaa ggaacgccat ttgaaactaa agttattgaa
     4741 gcggcaacgt tagatttact aacggcattt ttagatgaac agtatcagaa acaagaacaa
     4801 gattatagtc aaaattatgc atatgtacgc aaagtaggac ataccgtttt caaatgggtt
     4861 gctatcggta tgacaacgtt aagtgtttta ttaattgcat tcttagcctt tttatatttt
     4921 tcagtaatga agcataatga gcgcattgaa aaaggatacc aagcatttgt aaaggatgat
     4981 tatacgcaag tactaaatac gtatgatgat ttagatggta aaaaattaga taaagaggca
     5041 ctttacattt atgccaaaag ttatatccaa acaaataaac aaggtttaga aaaagataag
     5101 aaagaaaatt tacttaataa tgtgacacca aattcaaaca aagactactt attatattgg
     5161 atggaattag gacaaggaca tcttgatgaa gcgattaata ttgccactta tttagatgat
     5221 aacgatatta caaagttagc gttgattaat aaattaaatg agattaaaaa taacggagat
     5281 ttatcgaatg ataaacgttc tgaagaaacg aaaaagtata acgataaatt gcaagatatt
     5341 ttagacaaag aaaaacaagt taaagatgaa aaagcgaaat ctgaagaaga gaaagcaaaa
     5401 gcgaaagatg agaaattaaa gcaacaagaa gagaacgaaa agaaacaaaa agaacaagca
     5461 caaaaagata aagaaaaacg ccaagaagct gaaagaaaaa aatagtatag gactgaggca
     5521 aagacaatgc ataaattgat tataaaatat aacaaacaat tgaagatgct caatttgcga
     5581 gatggtaaga catatactat tagcgaagac gagcgtgcag atattacgtt gaaatcgtta
     5641 ggcgaagtca ttcatttaga acaaaataat caaggtactt ggcaagcgaa tcatacttct
     5701 attaataagg tgcttgttag aaaaggtgac cttgatgaca ttacattaca gctttataca
     5761 gaagctgatt atgcatcatt tgcgtatcct tcaattcaag atacgatgac aattggacca
     5821 aatgcgtatg atgatatggt tattcaaagc ttgatgaatg ccatcattat taaagatttt
     5881 caatcaatac aagaatcaca atacgtacgc attgtgcacg ataaaaatac agatgtgtat
     5941 attaactatg aactacaaga gcaactaacg aacaaagctt acattggtga tcatatttat
     6001 gttgaaggga tatggctcga agtacaagct gatggtttaa atgtattgag tcagaataca
     6061 gtggcatcgt cattaattcg cttaacacaa gagatgccac atgcacaggc agatgattac
     6121 aatacgtacc atcgttcgcc aaggattatt caccgtgaac cgacggatga tattaagatt
     6181 gaaagaccgc cacagccaat acagaagaac aatacagtga tatggcgttc cattataccg
     6241 ccattagtaa tgattgcttt aactgttgtc atctttttag tgagaccaat tggtatttat
     6301 attttaatga tgattggtat gagttcagta acgatagtat ttggtattac aacgtatttc
     6361 tctgaaaaga aaaagtataa caaagatgtt gaaaaacgag agaaagatta caaagattat
     6421 ttggataata aatctaaaga aattaataaa gcgattaaag cacaacgttt tagtttgaat
     6481 taccattatc caacggttgc tgaaattaaa gatatcgttg aaacgaaagc accaagaata
     6541 tatgaaaaaa catcgcatca tcacgatttc ttacattata agttaggtat tgcgaatgta
     6601 gaaaagtcat tcaaattaga ttaccaagaa gaagaattta accaacgtcg tgatgaatta
     6661 ttcgacgatg ctaaagaatt gtatgaattt tacacagatg tagaacaagc accattaatc
     6721 aatgatttaa atcatggacc gattgcatat attggtgcac gacatctcat tttagaagaa
     6781 ttggaaaaaa tgttaatcca attgtcaata ttccatagtt atcatgattt agagtttcta
     6841 tttgtgacac gtgaagatga agttgaaaca ttgaaatggg cacgttggtt gccacatatg
     6901 acattgagag gtcaaaacat tagaggattt gtttacaatc aacgaacacg tgaccaaatt
     6961 ttaacgtcaa tttacagcat gattaaagaa cgtatccaag ctgtgcgtga acgcagtaaa
     7021 agtaatgagc aaattatttt tacaccgcaa ttagtgtttg tcattacaga tatgtcatta
     7081 attattgatc atgtcatttt agaatatgta aaccaagatt tatcagaata tggtatttca
     7141 ttaatctttg ttgaagatgt gattgaaagt ttgccagagc atgtagatac cattattgat
     7201 atcaagtctc gtactgaagg agaactgatt acgaaagaaa aagaattagt tcaattgaaa
     7261 tttacacctg aaaatattga taacgtcgat aaagaatata tcgcgcgacg tttggcgaat
     7321 ttgatacacg tcgaacattt gaaaaatgca attcctgata gtattacatt tttagagatg
     7381 tataacgtga aagaagtaga tcagcttgat gtggttaatc gatggagaca aaacgaaaca
     7441 tacaaaacga tggcagtacc tttaggtgta agaggtaaag atgacatttt atcattgaac
     7501 ttacatgaaa aagcacacgg accacatggt ttagttgctg gtaccactgg gtcagggaaa
     7561 tctgagatta tccaatcata cattttatct ttagctatta attttcaccc acatgaagtt
     7621 gcgttcctat tgattgacta taaaggtggg ggtatggcga acttatttaa agatttagtc
     7681 catttagttg gtacgattac aaacttagat ggtgatgaag cgatgcgtgc cttaacatca
     7741 atcaaagccg aattgagaaa acgtcaacgt ttattcggag agcatgatgt taaccatatt
     7801 aatcaatacc ataagttatt taaagaaggt attgcgacag aaccaatgcc acatttattc
     7861 attatttccg atgagtttgc cgaattaaaa tcagaacaac ctgattttat gaaagaactt
     7921 gtatcaacgg cacgtattgg acgttcgtta ggtattcatt taatacttgc gacacaaaaa
     7981 ccatcgggtg ttgttgatga ccaaatttgg tctaactcta aatttaaatt ggcattaaaa
     8041 gtacaagata gacaagacag taatgaaatt ttaaaaacac cagatgcagc agacattaca
     8101 ttaccaggtc gtgcgtattt acaagttggt aataatgaaa tttatgaatt gttccaatct
     8161 gcatggagtg gagcaacata tgacatcgaa ggcgataaat tagaagttga agataagacg
     8221 atttacatga ttaatgatta tggtcaactt caagcaatca acaaagactt gagtggactt
     8281 gaagatgaag aaacgaaaga aaatcaaact gagttagaag cggttatcga tcatatagaa
     8341 tctattacaa cacgattaga aattgaagaa gttaagcgtc catggttacc accattacca
     8401 gaaaatgtat atcaagaaga tttagtagaa acagatttca gaaaattatg gtcagatgat
     8461 gcaaaagaag tggaattaac attaggactt aaagacgtac cagaagaaca atatcaaggg
     8521 ccgatggtat tacaattgaa aaaagctggt cacatcgcgt taatcggaag tccaggatat
     8581 ggtagaacaa cgttcttaca caacattatt ttcgatgttg caagacacca tcgtcctgat
     8641 caagcacaca tgtacttgtt cgatttcggt accaatggtt tgatgccagt cacagacata
     8701 ccacatgtcg ctgattactt tacagtagat caagaagaca agattgcgaa ggcgatacgt
     8761 atatttaatg atgaaatcga tcgccgtaag aagattttaa gtcagtatcg tgttactagt
     8821 atttctgaat atcgaaaatt aactggtgaa acaattccgc atgtctttat tcttattgat
     8881 aactttgacg cagtaaaaga ttcacctttt caagaagttt ttgaaaatat gatgattaaa
     8941 atgacacgtg aagggctagc attagacatg caagtaacct taactgcctc aagagctaac
     9001 gctatgaaaa caccaatgta cattaatatg aaaacgcgta tcgccatgtt tttatatgat
     9061 aaatcagaag tgtcgaacgt agtaggacag caaaaatttg cggttaaaga tgtagtgggt
     9121 cgagcattgt taagtagcga tgacaacgta tcattccata ttggccaacc atttaaacat
     9181 gatgagacca aatcatataa tgatcaaatt aatgatgaag tatcggcgat gacagaattt
     9241 tataaaggtg aaacaccaag tgatattcct atgatgccag atgaaattaa atatgaagat
     9301 tacagagaat cattaagctt accggatata gttgcaaatg gtgctttacc aattggatta
     9361 gattatgaag gtgttacact acaaaaaatt aaattaactg aaccagcaat gatttcatca
     9421 gaaaatccga gagaaattgc gcatattgct gaaattatga tgaaagaaat tgacatatta
     9481 aatgaaaaat atgcgatttg tatcgcagat tcaagtggag agtttaaagc ttatagacat
     9541 caagtggcta actttgccga agaaagagaa gacattaaag cgattcatca actaatgatt
     9601 gaagacttaa aacaaagaga aatggacggt ccgtttgaaa aagattcact ttacattatc
     9661 aatgatttta aaacatttat tgattgcacg tatattccgg aagatgatgt taaaaaactt
     9721 attacaaaag gaccagaact tggcttgaac attttatttg tcggcattca taaagaatta
     9781 atagatgctt atgataaaca gattgatgtt gcacgtaaaa tgattaacca atttagtata
     9841 ggtattcgta tttcagacca acaattcttt aaatttagat ttattcaacg agaacctgtt
     9901 attaaagaaa atgaagcata tatggtcgca aaccaagctt atcaaaagat tagatggttt
     9961 aaatagcaat gaattaaata ggagggaggt atgttatgaa ttttaatgat attgaaacaa
    10021 tggttaagtc gaaatttaaa gatattaaaa agcatgctga agagattgcg catgaaattg
    10081 aagttcgttc tggatattta agaaaagctg aacaatataa gcgattagaa tttaatttga
    10141 gttttgcact agatgatatt gaaagcacag caaaggacgt acaaactgca aaaactagtg
    10201 ctaataagga cagtgtaact gttaaaggaa aagcgcccaa tacgttatat atcgaaaaaa
    10261 gaaatttgat gaaacaaaag cttgaaatgt tgggtgaaga tatcgataaa aataaagaat
    10321 ccctccaaaa agctaaggaa attgctggag aaaaggcaag tgaatatttt aataaagcaa
    10381 tgaattaata ttgaggtgaa gatatgggtg gatataaagg tattaaagca gatggtggca
    10441 aggttgatca agcaaaacaa ttagcggcaa aaacagctaa agatattgaa gcatgtcaaa
    10501 agcaaacgca acagctcgct gagtatatcg aaggtagtga ttgggaagga cagttcgcca
    10561 ataaggtgaa agatgtgtta ctcattatgg caaagtttca agaagaatta gtacaaccga
    10621 ttgctgacca tcaaaaagca attgataact taagtcaaaa tctagcgaaa tacgatacat
    10681 tatcaattaa gcaagggctt gatagggtga acccatgatg aaagatgtta agcgaataga
    10741 ttatttttct tacgaagaat taacaatttt aggtggtagt aaattacctc tcgtaaattt
    10801 tgaattgttt gatccatcaa attttgaaga agctaaagcg gctttaattg aaaaggaatt
    10861 agtaacagag aatgacaagt taactgatgc aggttttaaa gtggctacat tagtcagaga
    10921 gtatattagc gcaattgtaa atattcgaat taatgatatg tattttgcac catttagcta
    10981 tgaaaaagat gaatatattt tgttaagccg gtttaaaaat aatggatttc aaatacgaat
    11041 tataaataaa gacattgcat ggtggtcgat tgtacaatca tatcctttat taatgagaca
    11101 agaaaagtcc aatgattggg actttaaaca aattgacgat gaaacattgg agaacttaaa
    11161 taatgaaagt atcgatacga ttgggcgtgt tttagaaatt gaaatataca atcatcaagg
    11221 tgaccctcaa caaagtttat ataacattta tgaacaaaat gatttgctac tcattcgata
    11281 cccattaaaa gataaagtac tgaatgttca tattggcgtc attaatacat ttatacgaga
    11341 attatttgga ttcaatactg atgaaaatca tattaataaa gcagaggagt aatgacgttg
    11401 agtggaaaaa ttagtgttaa agctgaaacg attgcacatg ttgtaaaaga attggaaagc
    11461 attagtcaaa agtatgatga aatagctcaa aactttggaa aaattgcgca attaaattac
    11521 tacagtagtg aaaaagcttc acattctatg gaaaacggct atagtagtgc tgcaacagtc
    11581 attagtggtc tcaaaggtcc attgagtaca ctcggtggtg gtgtcatgaa ttcagcacaa
    11641 aagttctttg aagcagatga acattggggt acggaatttg ccaagcttta ctataatatt
    11701 gagggatagg tgcatgacat gacaaaagat attgaatatt taacagctga ttatgacaat
    11761 gaaaagtcat ctatccaaag tgtaatagat gcaatagagg ggcaagactt cttagatgta
    11821 gatacaacaa tggatgatgc ggtaagcgat gtcagttctt tagacgaaga tggcgcaata
    11881 tcattaacaa gtagtgtagt aggtccacaa ggatctaaat taatgggcta ttatcaaaat
    11941 gagttatatg attatgcatc tcaattagat tcgaaaatga aagaaattat tgacacgcca
    12001 tttatagaag atatagataa agcattcaaa ggtataacga atgttaaatt ggaaaatata
    12061 ttaattaaaa atggcggtgg tcatggtaga gatacctatg gggcttctgg gaaaattgca
    12121 aagggagatg ccaagaaaag tgacagcgat gtttatagca tcgatgaaat attaaaatcg
    12181 gatcaagaat ttgtaaaagt aattgatcag cattacaaag aaatgaaaaa agaagataag
    12241 aaattatcta agagtgattt tgaaaaaatg atgactcagg gcgcttcttg tgattacatg
    12301 acagtagctg aagcggaaga gctagaggag caaaagaaaa aagaagaagc tatagagatt
    12361 gcagcactag ctggtatggt agttttatct tgtattaatc ctgttgctgg agcagtagct
    12421 attggtgctt attccgccta ttcagcagca aatgcagcca caggaaaaaa tattgtaact
    12481 ggaagaaagc tatctaaaga agaacgaatc atggaaggac tttcgcttat tccattgcca
    12541 ggtatgggct tcctcaaagg tgctgggaaa agtttaatga aattaggctt caaaggcgga
    12601 gaaaaatttg cagttaaaac aggattgcaa aagacaatgc aacaagcggt tagtcgtatt
    12661 tcacctaaaa tgggaatgat gaaaaacagt gtgttgaatc aatctcgtaa ctttgctcaa
    12721 aatactcatg ttggacaaat gctgagtaac atgcgtggtc aagcaactca tactgttcaa
    12781 caaagtagaa attggattgg acaacaagcg caaaatgtca aacgaatagt gaataatgga
    12841 cttgataaag aaatagcaca tccatttaaa caacaacttg caccagcggg aatgggtggt
    12901 ataaaatttg ctgaaacaac tactttgaga aacatgggtc aaaacatgaa acgtgctgtt
    12961 acaccacaaa atcacgtgac acatggtccg aaagatagta tggtgagaag tgaaggtaaa
    13021 catagtgtaa gtagccatga aataaattca tcaaaatatg ttgaatcacc aaactacacc
    13081 aaggttgaat tcggagaaca ctatgcaaga cttagaccta agaaactaaa agcaaatata
    13141 gaatatacaa caccaactgg tcacatatat cggaccgatc ataaagggcg cataaaagaa
    13201 gtttatgtag acaatctttc tctaaaagat ggcggtcgta ataaccatgc acaaagaact
    13261 gtggggggag aggatagatt accagacgat gatggaggtc atttaatcgc tagaatgttt
    13321 ggtgggtcaa aagacataga taaccttgtg gcacaaagta aatttatcaa ccgttcattt
    13381 aaggaaaatg gacaatggta taagttggaa aaactatggg aaaaagcaat caagtctggg
    13441 aagtcaatag aaaatgttaa agttgaagtt aaatataaag gtaatagtca aaggccaacc
    13501 gaattttcag ttagatataa tattaatggt gagcagtttt taaaaaatat aattaataaa
    13561 taggaggatt aacatgactt tcgaagaaaa actaagtcaa atgtataacg agattgcgaa
    13621 taagattagc agtatgatac cggtagaatg ggaaaaggta tatgcaatgg catatataga
    13681 tgatgaagga ggcgaagtgt tctactatta cacagaacct ggaagtaatg aattatacta
    13741 ttatactagt gtattaaata aatatgatat atcggaatca gaatttatgg attcagcgta
    13801 tgagttgtat aaacaatttc aaaagttaag aaatatattt aaaaaagaag gacatgaacc
    13861 atggacatca tgcgaatttg attttacaaa tgaaggtaaa ttaaaagttt catttgatta
    13921 tattgattgg ataaatacag agtttgatca attgggccgt caaaattatt atatgtacaa
    13981 aaaatttggg gttataccag aaatggaata tgaaatggag gaggttaaac aaatcgagca
    14041 atatattaaa gagcaagaag aagaatagac aatcttaatt tgagactaaa caataaagct
    14101 ttgtttagtc ttttttggcg gttaagtaaa aagcaataga gaccgtaaag ttgatgctca
    14161 tcaaataata atataaagat aattttaggt ttttaaactt ttaatcgaaa agaatctaag
    14221 tatatatagc tttaaatacg taatatgcta tttaaaagat aatgtatagg agatagatat
    14281 gactactaaa gaaaatattg atactcttcg aaagccaggt gcacaagcct taagtttaat
    14341 atcattgttt ttgatacttt tttcatgtct aactttcttt tttggtttag attatgaaag
    14401 gtttccaaac tatttaaaga taacgacaat tatagaatta ataattattg taattagttt
    14461 acttcaatgg attagattta tagacttcga aaaagaaagt acacagaaat ataaaaaaat
    14521 atatgctcga tttttagtta ttataaatgt gttaactact atcactgtag tatttgcact
    14581 gtgtaacctt tattattttg cagctgtaca aaaccattac gatttattca attattggtt
    14641 aatgggtacg atttcaatca taattagcta tttattatta gtaattggcg gaatgtttac
    14701 gttgttaaaa ttacctaaag taacaaaacg ctggggggta aaactaaaac acatttcggt
    14761 ttattattaa ctgcgttgag ctcatttata tatattgaaa aaattatcga atatatattg
    14821 attcctaatg tcgtagaatc caagtttata attattgtga gcatgatggt tatcgccgga
    14881 gcacaatttg tggcatttca atttattatg caatacagta gattctatat ttttgaatta
    14941 aacactgaag atgatgacta aatgaattta taaattactg ttaaagtata cttaaataag
    15001 aaaatcaact tcaacgtata tcgccatatt attttatact caatgatgtg aatggattta
    15061 agtggattga tattaaagta aaagttatgg aatattgagg tgcgaatatg gagttcttat
    15121 tattaattgt cgt
//
LOCUS       DAMZKC010000053        15116 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.053, whole genome shotgun sequence.
ACCESSION   DAMZKC010000053 DAMZKC010000000
VERSION     DAMZKC010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 15116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15116
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.053"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            208..609
                     /locus_tag="POB55_001233"
     CDS             208..609
                     /locus_tag="POB55_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDI6500203.1"
                     /translation="MKKVNNDTVFGILQLETLLGDINSIFSEIESEYKMSREEILILL
                     TLWQKGSMTLKEMDRFVEVKPYKRTRTYNNLVELEWIYKERPVDDERTVIIHFNEKLQ
                     QEKVELLNFISDAIASRATAMQNSLNAIIAV"
     gene            complement(729..1292)
                     /locus_tag="POB55_001234"
     CDS             complement(729..1292)
                     /locus_tag="POB55_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDI6500204.1"
                     /translation="MKLERILPFSKTLIKQHITPESIVVDATCGNGNDTLFLAEQVPE
                     GHVYGFDIQDLALENTRDKVKDFNHVSLIKDGHENIEHHINDAHKGHIDAAIFNLGYL
                     PKGDKSIVTKPDTTIQAINSLLSLMSIEGIIVLVIYHGHSEGQIEKHALLDYLSTLDQ
                     KHAQVLQYQFLNQRNHAPFICAIEKIS"
     gene            complement(1289..2242)
                     /locus_tag="POB55_001235"
     CDS             complement(1289..2242)
                     /locus_tag="POB55_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500380.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HDI6500205.1"
                     /translation="MGNHFQYAFENKRYHTWNYHLKNKFGQKIFKVALDGGFDCPNRD
                     GTVAHGGCTFCSAAGSGDFAGNRADSIAVQFKEIKEKMHEKWHEGKYIAYFQAFTNTH
                     APVEVLKEKFEPVLKEPGVVGLSIGTRPDCLPDDVVEYLADLNQRTYLWVELGLQTIH
                     QSTSDLINRAHDMKTYYDGVAKLRKHNINVCTHIINGLPGEDYDMMMATAKEVAQMDV
                     QGIKIHLLHLLKGTPMVKQYDKGLLTFMTQEEYTNLVVDQLEVIPPEMIVHRITGDGP
                     IDIMVGPMWSVNKWEVLNGIDAELARRNSYQGLRYKSKVKQ"
     gene            2352..3533
                     /locus_tag="POB55_001236"
     CDS             2352..3533
                     /locus_tag="POB55_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6500206.1"
                     /translation="MNIPKSVWWLVIGMALNITGSSFLWPLNTIYMKQELGKSLTVAG
                     LVLMINSFGMVIGNLLGGSLFDKLGGYKTILIGTFTCLCSTTLLNFFHGWPWYAVWLV
                     MLGFGGGMIIPAIYAMAGAVWPNGGRQTFNAIYLAQNIGVAVGAAMGGFVAEFSFNYI
                     FLANLIMYVVFALVAVTQFNIEINAKVKYPTQLDITGKKNKARFISLVLICAMFAICW
                     VAYIQWESTIASFTQSINISMAQYSVLWTINGIMILVAQPLIKPILYLLKGNLKKQMF
                     VGIIIFMLSFFVTSFAENFTIFVVGMIILTFGEMFVWPAVPTIANQLAPDGKQGQYQG
                     FVNSAATVGKAFGPFLGGVLVDAFNMRMMFIGMMLLLVFALILLMVFKENNTQPKKID
                     A"
     gene            3821..6244
                     /locus_tag="POB55_001237"
     CDS             3821..6244
                     /locus_tag="POB55_001237"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HDI6500207.1"
                     /translation="MLNYNHNQIEKKWQDYWDENKTFKTNDNLGQKKFYALDMFPYPS
                     GAGLHVGHPEGYTATDIISRYKRMQGYNVLHPMGWDAFGLPAEQYALDTGNDPREFTK
                     KNIQTFKRQIKELGFSYDWDREVNTTDPEYYKWTQWIFIQLYNKGLAYVDEVAVNWCP
                     ALGTVLSNEEVIDGVSERGGHPVYRKPMKQWVLKITEYADQLLADLDDLDWPESLKDM
                     QRNWIGRSEGAKVSFDVDNTEAEGKVEVFTTRPDTIYGASFLVLSPEHALVNSITTDE
                     YKEKVKAYQTEASKKSDLERTDLAKDKSGVFTGAYAINPLSGEKVQIWIADYVLSTYG
                     TGAIMAVPAHDDRDYEFAKKFDLPIIEVIEGGNVEEAAYTGEGKHINSGELDGLENEA
                     AITKAIQLLEQKGAGEKKVNYKLRDWLFSRQRYWGEPIPVIHWEDGTMTTVPEEELPL
                     LLPETDEIKPSGTGESPLANIDSFVNVVDEKTGMKGRRETNTMPQWAGSCWYYLRYID
                     PKNENMLADPEKLKHWLPVDLYIGGVEHAVLHLLYARFWHKVLYDMGIVPTKEPFQKL
                     FNQGMILGEGNEKMSKSKGNVINPDDIVQSHGADTLRLYEMFMGPLDAAIAWSEKGLD
                     GSRRFLDRVWRLMVNEDGTLSSKIVTTNNKSLDKVYNQTVKKVTEDFETLGFNTAISQ
                     LMVFINECYKVDEVYKPYIEGFVKMLAPIAPHIGEELWSKLGHEESITYQPWPTYDEA
                     LLVDDEVEIVVQVNGKLRAKIKIAKDTSKEEMQEIALSNDNVKASIEGKDIMKVIAVP
                     QKLVNIVAK"
     gene            6266..6577
                     /locus_tag="POB55_001238"
     CDS             6266..6577
                     /locus_tag="POB55_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDI6500208.1"
                     /translation="MKSITTDELKNKLLESKPVQIVDVRTDEETAMGYIPNAKLIPMD
                     TIPDNLNSFNKNEIYYIVCAGGVRSAKVVEYLEANGIDAVNVEGGMHAWGDEGLEIKS
                     I"
     gene            6903..13463
                     /gene="sasC"
                     /locus_tag="POB55_001239"
     CDS             6903..13463
                     /gene="sasC"
                     /locus_tag="POB55_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored repetitive surface protein SasC"
                     /protein_id="HDI6500209.1"
                     /translation="MNLLKKNKYSIRKYKVGIFSTLIGTVLLLSNPNGAQALTTDNNV
                     QSDTNQATPVNSQDKDVANNRGLANSAQNTPNQSATTNQATNQALVNHNNGSIVNQAT
                     PTSVQSSTPSAQNNNHTDGNTTATETVSNANNNDVASNNTTLNVPNKTNENGSGGHLT
                     LKEIQEDVRHSSDKPELVAIAEPASNRPKKRSRRAAPADPNATPADPGAAAAGNGGAP
                     VAITAPYTPTTDPNANNAGQNAPNEVLSFDDNSIRPSTNRSVPSVTVVDNLPGFTLIN
                     GGKVGVLSHAMVRTSMFEAGSNRTYQAQGNVLALGRISGTDASNHGDFNGIEKSLTVN
                     PNSELIFEFNTMPTKNGQGATNVIIKNADTNDTIAEKTVEGGPTLRLFKVPDNVRNLK
                     IQFVPKNDAITDARGIYQLKDGYKYYSFVDSIGLHSGSHVYVERRTMEPTATNNKEFT
                     VTTSLKNNGNSGASLDTDEFVYKIQLPEGVEYVNNSLTKDFPSNNSGVDVNDMNVTYD
                     AANRVITIKSTGGGTTNSPARLMPDKILDLKYKLRVNNVPTPRTVTFNDTLTYKTYTQ
                     DFINSAAESHTVSTNPYTIDIIMNKDALQAEVDRRIQQADYTFASLDIFNDLKKRAQT
                     ILAENRNNVPLNKRVSQADIDTLTNQMQHTLIRSVDAENAVNQKADQMEDLVNQNDEL
                     TDEEKQAAIQVIEEHKGNIIGDIGDQTTDDGVTRIKDQGIQTLSGDTATPVVKPNAKK
                     AIRDKATKQREIINATPDATEDEIQDAINQLATDETDAIDNVTNATTNADVETAKNNG
                     INTIGSVVPQVTHKQAARDAINQATATKRQQINSNREATQEEKNAALNELTQATNHAL
                     EQINQATTNADVDNAKGDGLNAINPIAPVTVVKQAARDAVSHDAQQHIAEINANPDAT
                     QEERQAAIDKVNAAVTAANTNILNANTNAEVEQVKTNAIQGIQAITPATKVKTDAKNA
                     IDKSAETQHNTIFNNNDATLEEQQAAQQLLDQAVATAKQNINAADTNQEVAQAKDQGT
                     QNIVVIQPATQVKTDARNVVNDKAREAITNINATPGATREEKQEAINRVNTLKNRALT
                     DIGVTSTTAMVNSIRDDAVNQIGAVQPHVTKKQTATGVLNDLATAKKQEINQNTNATT
                     EEKQVALNQVDQELATAINNINQADTNAEVDQAQQLGTKAINAIQPNIVKKPAALAQI
                     NQHYNAKLAEINATPDATNDEKNAAINTLNQDRQQAIESIKQANTNAEVDQAATVAEN
                     NIDAVQVDVVKKQAARDKITAEVAKRIEAVKQTPNATDEEKQAAVNQINQLKDQAFNQ
                     INQNQTNDQVDTTTNQALNAIDNVEAEVVIKPKAIADIEKAVKEKQQQIDNSLDSTDN
                     EKEVASQALAKEKEKALAAIDQAQTNSQVNQAATNGVSAIKIIQPETKVKPAAREKIN
                     QKANELRAKINQDKEATAEERQVALDKINEFVNQAMTDITNNRTNQQVDDTTSQALDS
                     IALVAPEHIVRAAARDAVKQQYEAKKQEIEQAEHATDEEKQVALNQLANNKKLALQNI
                     NQAVTNNDVKRVETNGIATLKGVQPHIVIKPEAQQAIKASAENQVESIKDTPHATVDE
                     LDEANQLISDTLKQAQQEIENTNQDAAVTDVRNQTIKAIEQIKPKVRRKRAALDSIEE
                     NNKNQLDAIRNTLDTTQDERDVAIDTLNKIVNTIKNDIAQNKTNAEVDRTETDGNDNI
                     KVILPKVQVKPAARQSVGVKAEAQNALIDQSDLSTEEERLAAKHLVEQALNQAIDQIN
                     HADKTAQVNQDSIDAQNIISKIKPATTVKATALQQIQNIATNKINLIKANNEATDEEQ
                     NIAIAQVEKELIKAKQQIASAVTNADVAYLLHDEKNEIREIEPVISRKASAREQLTTL
                     FNDKKQAIEANIQATVEERNSILAQLQNIYDTAIGQIDQDRSNAQVDKTASLNLQTIH
                     DLDVHPIKKPDAEKTINDDLARVTALVQNYRKVSDRNKADALKAITALKLQMDEELKT
                     ARTNADVDAVLKRFNVALSDIEAVITEKENSLLRIDNIAQQTYAKFKAIATPEQLAKV
                     KVLIDQYVADGNRMIDEDATLNDIKQHTQFIVDEILAIKLPAEAMKVSPKVIQPAPKV
                     CTPIKKEETHESRKVEKELPNTGSEEMDLPLKEFALITGAALLARRRTKNEKES"
     gene            complement(13580..14848)
                     /locus_tag="POB55_001240"
     CDS             complement(13580..14848)
                     /locus_tag="POB55_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDI6500210.1"
                     /translation="MYQTIIIGGGPSGLMAAVAASEQSSSVLLIEKKKGLGRKLKISG
                     GGRCNVTNRLPYAEIIKNIPGNGKFLYSPFSIFDNESIIDFFESRGVKLKEEDHGRMF
                     PVSNKAQDVVDTLVTTIERQHVTIKEEEAVSRIEVNTDQTFTVHTQNNSYESHSLVIA
                     TGGTSVPQTGSTGDGYKFAQDLGHTITELFPTEVPITSAEPFIKSNRLKGLSLKDVEL
                     SVLKKNGKKRISHQMDMLFTHFGISGPAALRCSQFVYKEQKNQKTQHISMAIDAFPEL
                     NHEQLKQHITSLLSDTPDKIIKNSLHGLIEERYLLFMLEQAGIDENTTSHHLSNQQLN
                     DLVNMFKGFVFKVNGTLPIDKAFVTGGGVSLKEIQPKTMMSKLVPGLFLCGEVLDIHG
                     YTGGYNITSALVTGHVAGLYAGHYSHASME"
BASE COUNT         5560 a         2373 c         2780 g         4403 t
ORIGIN      
        1 tcttatttct aaatattaac tctattgatt attggttttt atacttattt aattttattc
       61 aactttgaca attgaataga aagcaagttt atttacactt gtagttttat gcataagtta
      121 gcacatacaa gtttcgggat tgttgggatg tttgttaata cttgtatggt agctaaatat
      181 gtgattatta attgggagat gtttagcatg aaaaaagtaa ataacgacac tgtatttggg
      241 attttgcaat tagaaacact tttgggtgac attaactcaa ttttcagcga gattgaaagc
      301 gaatacaaaa tgtctagaga agaaatttta attttactaa ctttatggca aaaaggttct
      361 atgacgctta aagaaatgga cagatttgtt gaagttaaac cgtataagcg tacgagaacg
      421 tataataatt tagttgaatt agaatggatt tacaaagagc gtcctgttga cgatgaaaga
      481 acagttatta ttcattttaa tgaaaagtta caacaagaga aagtagagtt gttgaatttc
      541 atcagtgatg cgattgcaag tagagcaaca gcaatgcaaa atagtttaaa cgcaattatt
      601 gctgtgtaag tttaatagca taaaaagagg ttttcattaa gttgaaaacc tctttttgtt
      661 gttggtatta atttttcaaa tgttgactac tcaatcctaa attataaata gtatagcgca
      721 gcaaatgctt aagaaatttt ttctatggca caaatgaatg gagcatgatt acgttggttt
      781 aaaaattgat attgcaaaac ttgcgcatgc ttttgatcta aagtactcaa gtaatcaagc
      841 aatgcatgct tctcaatttg tccttcgcta tgaccatgat atataacaag tacaataata
      901 ccttcaattg acattaatga tagcaatgaa ttaatagctt ggattgtcgt gtcaggcttt
      961 gtcacgatag atttatcacc tttaggcaaa taacctaggt taaagatggc tgcatcaata
     1021 tgacctttat gtgcatcatt tatatgatgt tcaatatttt catgtccatc ttttattaaa
     1081 gaaacatgat tgaaatcctt aactttatca cgtgtatttt ccaaagctaa atcttgaatg
     1141 tcgaaaccat aaacatgtcc ttctggtact tgttcggcta aaaataaagt gtcattgccg
     1201 ttaccgcaag ttgcgtctac aacaatactt tctggtgtta tatgttgttt aataagtgtt
     1261 tttgaaaaag ggagtatacg ttctaatttc attgcttcac cttagacttg taacgcaagc
     1321 cttgatatga atttctacgt gctaattcag catcgatgcc atttaatact tcccatttat
     1381 taacactcca cattggaccg accatgatat ctattggacc atcaccggta attcggtgaa
     1441 cgatcatttc agggggaatc acttctaatt ggtccacaac taggtttgtg tactcttctt
     1501 gagtcataaa agttaataaa cctttatcgt attgttttac catcggtgta ccttttaaca
     1561 aatgaagtaa atgaatttta ataccttgta catccatttg tgcaacttct ttggcagtag
     1621 ccatcatcat gtcatagtct tcgccaggta agccattaat gatgtgtgta catacattga
     1681 tattatgctt acgtaatttt gccacaccat cataataagt tttcatatca tgggcacgat
     1741 tgattaaatc agatgttgat tgatggattg tttgtagtcc taattcaacc cataagtatg
     1801 ttcgttgatt caaatctgct aaatattcga caacatcgtc tggtagacag tcaggacgcg
     1861 taccaataga taatcctaca acacccggtt ctttaagtac aggttcgaat ttttctttta
     1921 atacttcaac tggcgcatgt gtatttgtaa atgcctgaaa ataagcaata tattttcctt
     1981 cgtgccattt ctcatgcatc ttttctttaa tttctttaaa ttgtactgcg attgaatctg
     2041 cacgattacc tgcaaagtct ccgctacctg cagcagaaca aaatgtacat ccaccatgtg
     2101 ctacagtgcc atcgcggtta ggacagtcaa acccgccatc caatgcaact ttaaatattt
     2161 tttgtccaaa tttatttttt aaatggtaat tccatgtgtg ataacgtttg ttttcaaaag
     2221 cgtattggaa atgattgccc atatgtcatt ttcctttcta taaaaaaaga gttctaagta
     2281 cagattttaa catattttaa tgttatagtg tttattatag tttgacaaaa aagagagagg
     2341 aactatgaaa tatgaatata cctaaatcag tctggtggct agtaattggc atggcgttaa
     2401 atattactgg ttccagtttt ttgtggcctt taaatacaat ttatatgaaa caagaacttg
     2461 gaaaaagttt aactgttgct ggtttagtgc taatgataaa ttcatttggc atggttattg
     2521 gaaacttatt aggtggttca ctatttgata aattaggtgg atacaagacg attttaattg
     2581 gaactttcac ttgtctttgt agtacaacgc tacttaattt ctttcacggg tggccttggt
     2641 atgctgtatg gcttgtaatg ttagggtttg gtggcggaat gattattcct gcgatatacg
     2701 ctatggctgg agcagtgtgg ccaaatggcg gaagacaaac gtttaatgcg atatacttag
     2761 cgcaaaatat tggtgtggct gtcggtgctg caatgggcgg ctttgtcgca gaatttagct
     2821 ttaactatat ctttttagcc aatcttatta tgtatgttgt gtttgcgctt gtcgcggtaa
     2881 cgcaatttaa tattgaaatt aatgcgaaag ttaaatatcc aactcaatta gatattactg
     2941 gtaaaaagaa taaagcaaga tttatttcat tagtactaat ttgtgcaatg tttgcaattt
     3001 gttgggttgc atatattcaa tgggagtcta caatcgcttc atttacacaa tctattaata
     3061 tttcaatggc acaatatagt gttttatgga caattaacgg aataatgatt ttagtagcac
     3121 aaccattaat taaaccgatt ctctatctgt taaaaggaaa cttaaagaag caaatgtttg
     3181 tcggcatcat catttttatg ttgtcgttct ttgtcacgag ttttgccgaa aactttacaa
     3241 tatttgttgt cggtatgatt attttaactt ttggagaaat gtttgtatgg ccagcagttc
     3301 caactatagc caatcagtta gcgccagatg gtaagcaagg acagtaccaa ggttttgtga
     3361 attcagctgc tacagtagga aaagcatttg gtccatttct tggtggtgta ttagttgatg
     3421 cgtttaatat gcgcatgatg tttatcggta tgatgctact acttgtattt gcattaatat
     3481 tattaatggt tttcaaggag aataatacgc aacctaaaaa aatagatgca taatgagtaa
     3541 atagaattaa cgttatagac ttgaaataaa tgtcgttata acataatatt aatttgtata
     3601 atttaatttc gtttggagct tttctacaga aagctagtga tgctgagagc tagtgttaag
     3661 gactaaatgt aaatcgtatt aattttaaat tgaatgaatg acatctctta ctattaaaat
     3721 gagtgcacaa tttttgtgaa atagggtggt aacgcggcaa atgtcgtccc tatgtaaata
     3781 gaatagttag aggtgtcttt tttattgaat aggaggaaat gtgttgaatt acaaccacaa
     3841 tcaaattgaa aagaaatggc aagactattg ggacgaaaat aaaacattta aaacaaatga
     3901 taacttaggt caaaagaaat tttatgcttt agacatgttt ccatatccat caggtgctgg
     3961 tttacatgtt ggacatcctg agggctatac agcaacagat atcatttcaa gatataaaag
     4021 aatgcaagga tataatgtat tacatccgat ggggtgggat gcattcggat taccagcaga
     4081 gcaatatgct ttagacactg gcaacgaccc acgtgaattt acaaagaaaa atatccaaac
     4141 ttttaaacga caaattaaag aattagggtt cagttatgat tgggatcgtg aagttaatac
     4201 aacagatcca gaatactata aatggacaca gtggattttc atacagttat ataacaaagg
     4261 tttagcatac gttgatgaag ttgcagttaa ctggtgtcca gcattaggca ctgttttatc
     4321 taacgaagaa gtgattgatg gtgtctctga acgtggtgga catccagttt atcgtaagcc
     4381 gatgaaacaa tgggtactta aaatcacaga atatgcagat caattattag cagatttaga
     4441 tgatttagat tggcctgaat ctttaaaaga tatgcagcgc aattggattg gacgttctga
     4501 aggggccaaa gtttcatttg atgtagataa tacggaagcg gaaggaaaag tagaagtatt
     4561 tacgactaga ccagatacaa tctatggtgc atcattctta gtcttaagtc ctgaacatgc
     4621 attagttaat tcaattacaa ctgatgaata taaagaaaaa gtaaaagctt atcaaacaga
     4681 agcttctaaa aagtcagatt tagaacgtac agatttagca aaagataaat caggtgtgtt
     4741 tactggtgca tatgcaatta atcctttatc tggtgaaaaa gtacaaattt ggattgctga
     4801 ttatgtatta tcaacatatg gtactggagc aattatggca gtaccagcgc atgatgacag
     4861 agattatgaa tttgctaaaa agtttgattt gccaatcatt gaagtcatcg aaggtggaaa
     4921 tgttgaagaa gcagcataca ctggtgaagg taaacatatt aattctggtg aacttgatgg
     4981 tttagaaaat gaagcggcaa ttactaaagc tattcaatta ttagagcaaa aaggtgctgg
     5041 cgaaaagaaa gttaattaca aattaagaga ttggttattc agtcgtcagc gttattgggg
     5101 cgaaccaatt cctgtcattc attgggaaga tggaacaatg acaactgttc ctgaagaaga
     5161 gctaccattg ttgttacctg aaacagatga aatcaagcca tcagggactg gtgagtctcc
     5221 actagctaat attgattcat ttgtaaatgt tgtagatgaa aaaacaggta tgaaaggacg
     5281 tcgtgaaaca aatacaatgc cacaatgggc aggtagttgt tggtattatt tacgttacat
     5341 cgatcctaaa aatgaaaata tgttagcaga tcctgaaaaa ttaaaacatt ggttacctgt
     5401 tgatttatat atcggtggag tagaacatgc ggttcttcac ttattatatg caagattttg
     5461 gcataaagtc ctttatgata tgggtatcgt acctactaaa gaacctttcc aaaaattatt
     5521 taaccaaggt atgattttag gagaaggtaa tgagaagatg agtaaatcta aaggaaatgt
     5581 aatcaatcct gatgatatag tacagtctca tggtgcagat actttacgtc tttacgaaat
     5641 gtttatggga cctttagatg ctgcaattgc atggagtgaa aaaggattag atgggtctcg
     5701 tcgattctta gatcgcgtat ggcgtttaat ggtaaatgaa gatggaacat tgagttcaaa
     5761 aattgtaact acaaataata aatctttaga taaagtttat aaccaaactg ttaaaaaggt
     5821 aacagaagac tttgaaacat taggatttaa tactgctatt agtcaattaa tggtatttat
     5881 taatgagtgt tataaagttg atgaagttta taaaccttac attgaaggct tcgttaaaat
     5941 gttagcacct attgcaccac atatcggtga agaattatgg tcaaaattag gacatgaaga
     6001 gtctattacg taccaacctt ggccaactta tgacgaagca ctacttgtag atgatgaagt
     6061 agaaatcgtt gttcaagtaa atggtaaatt gagagctaaa attaaaattg ctaaagatac
     6121 atcaaaagaa gaaatgcaag aaattgcctt atctaatgac aatgttaaag cgagtattga
     6181 aggtaaagac atcatgaaag tcatcgctgt tcctcaaaaa ttagtcaata ttgtagctaa
     6241 ataatgtttt aaggaggact ttgaaatgaa gtcaattact acagatgaat taaaaaacaa
     6301 acttttagaa tctaaaccag ttcaaattgt tgatgttcgt actgacgaag aaacagcaat
     6361 gggatatatt cctaatgcaa agttgattcc aatggatacc attccggata atttaaattc
     6421 atttaataaa aatgaaatat attatattgt atgtgctggt ggagttcgaa gcgctaaagt
     6481 tgtagaatat ttagaggcaa atggcattga tgccgtaaat gtcgaaggcg gcatgcacgc
     6541 atggggcgat gaaggtttgg aaataaaaag tatttaaagt agtgacataa tttaaaataa
     6601 tattacattt gtaatgacac caagtaacgt ttcggttgct tggtgttttt tggtatgaat
     6661 tactttctgt tacaaaacaa tctaaagcgt tcttgttatg ttttattaag attttaatta
     6721 caaaacggaa actaaattgt aataaaataa aactttattt tataaaatga tgatgataaa
     6781 attgagtgaa cttaaaatat tgtacaaaat aatatagcta taaacataat gtgagggagc
     6841 gtatattttt agcataattc ttaacaacac agcagagaac agacaaccag gaggaaaatg
     6901 aaatgaattt gttaaagaaa aataaatata gtattaggaa gtataaagta ggcatattct
     6961 ctactttaat cggaacagtt ttattacttt caaacccaaa tggtgcacaa gccttaacta
     7021 cggataataa tgtacaaagc gatactaatc aagcaacacc tgtaaattca caagataaag
     7081 atgttgctaa taatagaggt ttagcaaata gtgcgcagaa tacacctaat caatctgcaa
     7141 caaccaatca agcaacgaat caagcattgg ttaatcataa taatggtagt atagtaaatc
     7201 aagctacgcc aacatcagtg caatcaagta cgccttcagc acaaaacaat aatcatacag
     7261 atggcaatac aacagcaact gagacagtgt caaacgctaa taataatgat gtagcgtcga
     7321 ataataccac attaaatgta ccaaataaaa caaatgaaaa tggttcagga ggacatctaa
     7381 ctttaaagga aattcaagaa gatgttcgtc attcttcaga taaaccagag ctagttgcaa
     7441 ttgctgaacc agcatctaat agaccgaaaa agagaagtag acgtgcggca ccggcagatc
     7501 ctaatgcaac tccagcagat ccaggggctg cagcggcagg aaacggtggt gcaccagttg
     7561 caattacagc gccatacacg cctacaactg atcctaatgc caataatgca ggacaaaatg
     7621 cacctaacga agtgctgtca tttgatgaca atagtattag accaagtacc aaccgttctg
     7681 taccatcagt aacagttgtt gataatttac caggctttac acttatcaat ggtggtaaag
     7741 taggtgtgct aagtcatgcg atggttagaa caagtatgtt tgaggctggg agcaacagaa
     7801 cctatcaggc acaaggcaat gtacttgcat taggacgtat aagcgggacg gacgctagta
     7861 atcatggaga ttttaatggt attgaaaaat ctttaacagt aaatccaaat tctgaattaa
     7921 tctttgaatt taatacaatg ccaactaaaa atggtcaagg cgcaacaaat gttattatca
     7981 aaaatgctga tactaatgat acgattgctg aaaagactgt tgaaggcggt ccaactttgc
     8041 gtttatttaa agtacctgat aatgtgagaa atctcaaaat tcaatttgta cctaaaaatg
     8101 acgcaataac agatgcgcgt ggcatttatc aactaaaaga tggttacaaa tactatagct
     8161 ttgttgactc tatcggactt cattctgggt cacatgttta tgttgaaaga cgaacaatgg
     8221 aacccacagc aacgaataat aaagaattta cagttacaac atcactaaaa aataatggta
     8281 attctggtgc ttctttagac acagatgaat ttgtttataa aattcaatta cctgaagggg
     8341 tagaatatgt gaacaattca ttgactaaag attttccaag taacaattca ggcgttgatg
     8401 ttaatgatat gaatgttaca tatgatgcgg cgaatcgagt gataacaatt aaaagcactg
     8461 gtggtggcac aacgaattca cctgcacgac taatgcctga taaaatattg gatttaaagt
     8521 ataagttgcg tgttaataat gtaccgacac caagaacagt aacatttaat gatacattaa
     8581 cgtataaaac atatacacaa gatttcatta attcagctgc agaaagtcat actgtaagta
     8641 caaatccata tactatcgat atcatcatga ataaagatgc attacaagcc gaagttgaca
     8701 gacgtattca acaagctgat tatacatttg catcattaga tatctttaat gatcttaaaa
     8761 aacgtgcaca aacaatttta gctgaaaatc gtaacaatgt accattaaat aaaagagtat
     8821 ctcaagcaga tattgataca ttaactaatc agatgcaaca tactttaatt cgaagtgttg
     8881 atgctgaaaa tgcagtaaat caaaaagctg atcaaatgga agatttagtt aatcaaaatg
     8941 atgaactgac agatgaagaa aaacaagcag caatacaagt tatcgaggaa cataaaggca
     9001 atattattgg tgatattggt gatcaaacga ctgatgatgg cgttactaga atcaaagatc
     9061 aaggtataca gaccttaagt ggggatactg caacaccggt tgttaaacca aatgctaaaa
     9121 aagcaatacg tgataaagca acgaaacaaa gggaaattat caatgcaaca ccagatgcta
     9181 ctgaagacga gattcaagat gcaataaatc aattagctac ggatgaaaca gatgctattg
     9241 ataatgttac gaatgctact acaaatgctg acgttgaaac agctaaaaat aatggcatca
     9301 atactattgg atcagttgtt cctcaagtga cacataaaca agctgcaaga gatgcaatta
     9361 accaagcaac agcaacgaaa agacaacaaa taaatagtaa tagagaagca actcaggaag
     9421 agaaaaatgc agcattgaac gaattaactc aagcaaccaa ccatgcttta gaacaaatca
     9481 atcaagcaac aacaaatgct gatgttgata acgccaaagg agatggtcta aatgccatta
     9541 atccaattgc tcctgtaact gttgttaagc aagctgcaag ggatgccgta tcacatgatg
     9601 cacaacaaca tatcgcagag atcaatgcta atcctgatgc gactcaagaa gaaagacaag
     9661 cagcaattga caaagtgaat gctgctgtaa ctgcagcaaa cacaaacatt ttaaacgcta
     9721 ataccaatgc tgaagtggaa caagtgaaga caaatgcgat acaaggaata caagcaatta
     9781 caccagctac aaaggtaaaa acagatgcaa aaaatgccat cgataaaagt gcggaaacgc
     9841 aacataatac gatatttaat aataatgatg cgacgctcga agaacaacaa gcagcacaac
     9901 aattacttga tcaagctgta gccacagcga agcaaaatat taatgcagca gatacgaatc
     9961 aagaagttgc acaagcaaaa gatcagggca cacaaaatat agtagtgatt caaccggcaa
    10021 cacaagttaa aacggatgct cgcaatgttg taaatgataa agcgcgagag gcgataacaa
    10081 atatcaatgc tacacctggc gcgactcgag aagagaaaca agaagcgata aatcgtgtca
    10141 atacacttaa aaatagagca ttaactgata ttggtgtgac atctactact gcgatggtca
    10201 atagtattag agacgatgca gtcaatcaaa tcggcgcagt tcaaccgcat gtaacgaaga
    10261 aacaaactgc tacaggtgta ttaaatgatt tagcaactgc taaaaagcaa gaaattaatc
    10321 aaaacacaaa tgcaacaact gaagaaaagc aagtggcttt aaatcaagtg gatcaagagt
    10381 tagcaacggc aattaataat ataaatcaag ctgatacaaa tgcggaagta gatcaagcgc
    10441 aacaattagg tacaaaagca attaatgcga ttcagccaaa tattgttaaa aaacctgcag
    10501 cattagcaca aatcaatcag cattataatg ctaaattagc tgaaatcaat gctacaccag
    10561 atgcaacgaa tgatgagaaa aatgctgcga tcaatacttt aaatcaagac agacaacaag
    10621 ctattgaaag tattaaacaa gctaacacaa atgcagaagt agaccaagct gcgacagtag
    10681 cagagaataa tatcgatgct gttcaagttg acgtagtaaa aaaacaagca gcgcgagata
    10741 aaatcactgc tgaagtggcg aagcgtattg aagcggttaa acaaacacct aatgcaactg
    10801 acgaagaaaa gcaggctgct gttaatcaaa tcaatcaact taaagatcaa gcatttaatc
    10861 aaattaatca aaaccaaaca aatgatcagg tagacacaac tacaaatcaa gcgttaaatg
    10921 ctatagataa tgttgaagct gaagtagtaa ttaaaccaaa ggcaattgca gatattgaaa
    10981 aagctgttaa agaaaagcaa cagcaaattg ataatagtct tgattcaaca gataatgaga
    11041 aagaagttgc ttcacaagca ttagctaaag aaaaagaaaa agcacttgca gctattgacc
    11101 aagctcaaac gaatagtcag gtgaatcaag cagcaacaaa tggtgtatca gcgattaaaa
    11161 ttattcaacc tgaaacaaaa gttaaaccag ctgcacgtga aaaaatcaat caaaaagcga
    11221 atgaattacg tgctaagatt aatcaggata aagaagcaac agcagaagaa agacaagtag
    11281 cactagataa aatcaatgaa tttgtaaatc aagccatgac agatattacg aataatagaa
    11341 caaatcaaca agttgatgat acaacaagtc aagcgcttga tagcattgct ttagtagcgc
    11401 ctgaacatat tgttagagca gctgctagag atgcagtaaa gcaacaatat gaggctaaaa
    11461 agcaagaaat agagcaagct gaacatgcga ctgatgaaga aaaacaagtt gctttaaatc
    11521 aattagcgaa taataaaaaa cttgcattac aaaacatcaa tcaagcagta acgaataatg
    11581 atgtgaaacg tgttgaaaca aatggcattg ctacactaaa aggtgtacaa cctcatattg
    11641 taattaagcc tgaagcacaa caagcaataa aagcaagtgc agaaaatcaa gtagaatcaa
    11701 taaaagatac accacatgca acagttgatg aattagatga agcgaatcaa ttaattagcg
    11761 acacactcaa acaagcgcaa caagaaatag aaaatacaaa tcaagatgct gctgttactg
    11821 atgttagaaa tcaaacaatc aaggcaattg agcaaataaa acctaaagta agacgtaaac
    11881 gagctgcgct tgatagcatt gaagaaaata ataaaaatca actcgatgca atccgaaata
    11941 cgttggatac tactcaagat gaaagagatg ttgctattga tactttaaat aaaattgtaa
    12001 atacaattaa aaatgacatt gcacaaaaca aaacgaatgc agaagtggat cgaactgaga
    12061 ctgatggcaa cgacaacatc aaagtgattt tacctaaagt tcaagttaaa ccagcagcgc
    12121 gtcaatctgt tggtgtaaaa gccgaagctc aaaatgcact aatcgatcaa agcgatttat
    12181 caactgaaga agaaagacta gctgctaaac atttagtaga acaagcactt aatcaggcta
    12241 ttgatcagat caatcatgca gataagactg cccaagttaa tcaagatagt atagatgctc
    12301 aaaatattat ttcaaaaatt aaaccagcga caacagttaa agcaacagca ttacaacaaa
    12361 ttcaaaatat cgctacaaat aaaattaatt taattaaagc aaataacgaa gcgacagatg
    12421 aagaacaaaa tattgcaata gcacaagttg aaaaagagtt aattaaagct aaacaacaaa
    12481 ttgctagtgc agtgactaat gctgatgtgg catatttatt gcatgatgag aaaaacgaaa
    12541 ttcgtgaaat cgaacctgtt attagcagaa aggcgtctgc tcgagaacaa ttgacaacat
    12601 tattcaacga taaaaaacaa gcaattgaag cgaatattca agcaacggta gaagaaagaa
    12661 atagtatatt agcacagtta caaaatattt atgacactgc tattggacaa attgatcaag
    12721 atcgtagcaa tgcacaagtt gataaaacag catcattaaa tctacaaaca atacatgatt
    12781 tagatgtaca tcctattaaa aagccagatg ctgaaaaaac gattaatgat gatcttgcac
    12841 gcgtcactgc tttagtgcaa aattatcgaa aagtaagtga tcgtaataag gctgatgcat
    12901 taaaagctat aactgcttta aaattacaaa tggatgaaga attaaaaaca gcacgcacta
    12961 atgctgatgt tgatgcagtt ttaaaacgat ttaatgttgc attaagcgat atagaagcag
    13021 taattactga aaaagaaaat agcttactgc gcattgataa cattgctcaa caaacatatg
    13081 cgaaattcaa agcgatcgca acaccagaac aattagctaa agtaaaagta ttaattgatc
    13141 aatatgttgc agatggcaat agaatgattg atgaagatgc gacattaaat gacatcaaac
    13201 aacatacgca attcattgtt gatgaaattt tagcaattaa attaccagct gaagcgatga
    13261 aagtatcacc aaaagtaatt cagccagctc caaaagtttg tacgcctatt aaaaaagaag
    13321 agacacatga atcgcgcaaa gttgaaaaag aacttccaaa tacaggttct gaagaaatgg
    13381 atttaccatt gaaagaattt gcactgatta caggtgcggc tttgttagct agaagacgta
    13441 ctaaaaacga aaaagaatca taattaacga aatgacctta atataacgac actttttagt
    13501 gactcagtta tattaaggtc atttttaatt gaatgaaaaa gttgatctac taaagagaat
    13561 cgaaccaaat tttatactat tattccattg atgcatgtga gtaatgtccg gcatataatc
    13621 cagcgacatg tcctgttacg agtgcacttg taatattata accaccagta taaccatgta
    13681 tatctaatac ttcaccacat aaaaataatc ccggaactaa tttagacatc attgttttag
    13741 gttgaatttc tttaagtgac acaccaccac ctgtgacaaa tgccttatct ataggtaatg
    13801 tcccgttcac cttaaataca aaccctttaa acatatttac taagtcgttc aattgttgat
    13861 ttgataagtg atgtgatgtg gtattttcat cgattcctgc ttgttccagc atgaacagta
    13921 agtagcgctc ttcaattaga ccatgcaaac tgtttttaat gattttatct ggtgtgtccg
    13981 ataataatga tgtgatgtgt tgttttaatt gttcatggtt taattcagga aatgcatcga
    14041 ttgccataga aatgtgctgt gtcttttgat ttttttgttc tttataaaca aactgactac
    14101 atcttaatgc agctggacca ctgataccaa aatgagtaaa taacatatcc atttgatgac
    14161 tgatgcgttt tttaccattt ttcttaagta ctgacaattc aacatctttt aaacttaaac
    14221 cttttagacg attggatttg atgaaaggtt cagctgatgt aattggaact tcggtcggga
    14281 ataactcagt aatggtatga cctaaatctt gtgcgaactt ataaccatca ccagttgaac
    14341 cagtttgagg gacacttgta ccacctgtag caatcactag cgaatggctt tcataactat
    14401 tattttgagt atgtacagtg aaagtttggt ctgtattaac ttcgattcta ctaacagctt
    14461 cttcttcttt aatcgttaca tgttggcgtt cgatagttgt cactaatgta tcaaccacgt
    14521 cttgtgcttt gttggaaact ggaaacatac gcccgtgatc ttcttctttt aatttaacac
    14581 ccctagactc aaaaaaatct atgatggatt cattatcaaa aattgaaaag ggactatata
    14641 aaaatttccc atttccagga atgttcttaa taatttcagc atatggtaat cgattagtta
    14701 cgttacatct accgccacca gatattttga gtttacgacc tagacctttc tttttttcaa
    14761 tgagtaacac actgctactt tgttcgcttg cagctactgc cgccattaag ccgctaggtc
    14821 cgcctccgat aataattgtt tgatacatat acgcttcctc caaatttgtt actggacatt
    14881 caattgctat tttatagtac ttttttaaac tttgctatat ctcttgtgtt atgagatgac
    14941 aattatgttg aatatattta taatgtgaac ttcataaaaa atgaagatgt atgtgtatga
    15001 taagaatgat ttttctactg ttatgcgatg ttcatctcaa atagtagtat aatgtttata
    15061 tgaaatttcg taatattcta atcaaaatct agtattcaat gacaatgtgt tgataa
//
LOCUS       DAMZKC010000054        14925 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.054, whole genome shotgun sequence.
ACCESSION   DAMZKC010000054 DAMZKC010000000
VERSION     DAMZKC010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14925
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.054"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            40..930
                     /locus_tag="POB55_001241"
     CDS             40..930
                     /locus_tag="POB55_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HDI6500211.1"
                     /translation="MNKISKYIAIASLSVAVTVSAPQTTNSTAFAKSSAEVQQTQQAS
                     IPASQKVNLGNQNIMAVAWYQNSAEAKALYLQGYNSAKVQLDKEIKKNKGKHKLAIAL
                     DLDETVLDNSPYQGYASIHNKPFPEGWHEWVQAAKAKPVYGAKEFLKYADKKDVDIYY
                     ISDRDKEKDLKATQKNLKQQGIPQAKKSHILLKGKDDKSKESRRQMVQKDHKLVMLFG
                     DNLLDFTDPKEATAESREALIEKHKDDFGKKYIIFPNPMYGSWEATIYNNNYKASDKA
                     KDKLRKNAIKQFDPKTGEVK"
     gene            1180..2229
                     /locus_tag="POB55_001242"
     CDS             1180..2229
                     /locus_tag="POB55_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDI6500212.1"
                     /translation="MFLAWNEIRRNKLKFGLIIGVLTMISYLLFLLSGLANGLINMNK
                     EGIDKWQAEAIVLNKDANQTVQQSVFNKKDIENKYKKQATLKQTGEIVSNGHQKDNVL
                     VFGVEKSSFLVPSLIEGHKATKDNEVLADETLKNKGFKIGDTLSLSQSDEKLHIVGFT
                     ESAKYNASPVIFTNDATIAKINPRLTGDKINAVVVRDTNWKDKKLNQELEAVSINDFI
                     ENLPGYKPQNLTLNFMISFLFVISATVIGIFLYVMTLQKTSLFGILKAQGFTNGYLAN
                     VVISQTLILALFGTAFGLLLTGVTGAFLPDAVPVKFDVLTLLVFAIVLMIVSVLGSLF
                     SILTIRKIDPLKAIG"
     gene            2242..2919
                     /locus_tag="POB55_001243"
     CDS             2242..2919
                     /locus_tag="POB55_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6500213.1"
                     /translation="MLKFENVTKSFKDGNRNIEAVKDTNFEINKGDIIALVGPSGSGK
                     STFLTMAGALQTPTSGHILINNQDITTMKQKALAKVRMSEIGFILQATNLVPFLTVKQ
                     QFTLLKKKNKNVMSNEDYQQLMSQLGLTSLLNKLPSEISGGQKQRVAIAKALYTNPSI
                     ILADEPTAALDTENAIEVIKILRDQAKQRKKACIIVTHDERLKAYCDRSYHMKDGVLN
                     LENETVE"
     gene            3127..4158
                     /locus_tag="POB55_001244"
     CDS             3127..4158
                     /locus_tag="POB55_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDI6500214.1"
                     /translation="MDLLIGTLFLFLVLVIFTLFTYKAPNGMRAMGALANAAIATFLV
                     EAFNKYVGGEVFGIKFLEELGDAAGGLGGVAAAGLTALAIGVSPVYALVIAAACGGMD
                     LLPGFFAGYMIGYVMKYTEKYVPDGVDLIGSIVILAPLARLIAVLLTPVVNSTLIRIG
                     DIIQSSTNTNPIIMGIILGGIITVVGTAPLSSMALTALLGLTGVPMAIGAMAAFSSAF
                     MNGTLFHRLKLGDRKSTIAVSIEPLSQADIVSANPIPIYITNFFGGAIAGLIIAMSGL
                     INDATGTATPIAGFLVMFGFNHPTTIVIYGVVMAIVGALAGYLGSIVFKKYPIVTKQD
                     MINRGAVDA"
     gene            4501..5619
                     /locus_tag="POB55_001245"
     CDS             4501..5619
                     /locus_tag="POB55_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDI6500215.1"
                     /translation="MSDSEKEILKRIKDNPFISQRELAEAIGLSRPSVANIISGLIQK
                     EYVMGKAYVLNEDYPIVCIGAANVDRKFYVHKNLVAETSNPVTSTRSIGGVARNIAEN
                     LGRLGETVAFLSASGQDSEWEMIKRLSTPFMNLDHVQQFENASTGSYTALISKEGDMT
                     YGLADMEVFDYITPEFLIKRSHLLKKAKCIIVDLNLGKEALNFLCAYTTKHQIKLVIT
                     TVSSPKMKNMPDSLHAIDWIITNKDETETYLNLKIESTDDLKIAAKRWNDLGVKNVIV
                     TNGVKELIYRSGEEEIIKSVMPSNSVKDVTGAGDSFCAAVVYSWLNGMSTEDILIAGM
                     VNAKKTIETKYTVRQNLDQQQLYHDMEDYKNGKFTKVY"
     gene            5594..6517
                     /locus_tag="POB55_001246"
     CDS             5594..6517
                     /locus_tag="POB55_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HDI6500216.1"
                     /translation="MANLQKYIEYSREVQQALENNQPIVALESTIISHGMPYPQNVEM
                     ATTVEQIIRNNGAIPATIAIIDGKIKIGLESEDLEILATSKDVAKVSRRDLAEVVAMK
                     CVGATTVATTMICAAMAGIQFFVTGGIGGVHKGAEHTMDISADLEELSKTNVTVICAG
                     AKSILDLPKTMEYLETKGVPVIGYQTNELPAFFTRESGVKLTSSVETPERLADIHLTK
                     QQLNLEGGIVVANPIPYEHALSKAYIEAIINEAVVEAENQGIKGKDATPFLLGKIVEK
                     TNGKSLAANIKLVENNAALGAKIAVAVNKLL"
     gene            6528..7748
                     /locus_tag="POB55_001247"
     CDS             6528..7748
                     /locus_tag="POB55_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDI6500217.1"
                     /translation="MNILFAITGIAFALFVAFLFSFDRKNIDFKKTLIMIFIQVLIVL
                     FMMNTTIGLTILTALGSFFEGLINVSKAGINFVFGDIQNKNGFTFFLNVLLPLVFISV
                     LIGIFNYIKVLPFIIKYVGIAINKITRMGRLESYFAISTAMFGQPEVYLTIKDIIPRL
                     SRAKLYTIATSGMSAVSMAMLGSYMQMIEPKFVVTAVMLNIFSALIIASVINPYKSDD
                     SDVEIDNLTKSTETKSVNGKTGKPKKVAFFQMIGDSAMDGFKIAVVVAVMLLAFISLM
                     EAINIMFGSVGLNFKQLIGYVFAPIAFLMGIPWSEAVPAGSLMATKLITNEFVAMLDF
                     KNVLGDVSARTQGIISVYLVSFANFGTVGIIVGSIKGISDKQGEKVASFAMRLLLGST
                     LASIISGSIIGLVL"
     gene            complement(7854..9386)
                     /locus_tag="POB55_001248"
     CDS             complement(7854..9386)
                     /locus_tag="POB55_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HDI6500218.1"
                     /translation="MKEIGFGTLNWVAVIIYLLAMLFIGVYFTKRASQSTNSFFTASG
                     RLPSWVVGFSIYATTLSAITFMSTPEKAFLTDWSYIAGNIAIVAIIPLLIYFYVPFFK
                     KLKVTSAYEYLEARFGPSIRVIGSLLFVVYHLGRVAIVIYLPTLAITSVSDMNPYIVA
                     SLVGLLCILYTFLGGFEGVVWSDFIQGVILLGGALVIIILGVMNIKGGFGTVFADAIE
                     HKKLISADNWKLNTAAAAIPIIFLGNIFNNLYQYTASQDVVQRYQASDSLKETNKSLW
                     TNGILALISAPLFYGMGTMLYSFYTHEAVLPKGFNTSSVVPYFILTEMPPFVAGLLIA
                     AIFAAAQSTISSSLNSISACISIDIKQRFFGKGSERHEVNFARFIIIIAGIFGFGMSL
                     YLIASNSNDLWDLFLFVTGLFGVPLAGVFAVGIFTKRTNTFGVICGLILGIIFAYVYN
                     GVGKGNSPFYVSTISFTVAFVFAYILSFIVPSKHKKDITGLTIFEKDKPSTYISKMAT
                     KK"
     gene            complement(9426..10307)
                     /locus_tag="POB55_001249"
     CDS             complement(9426..10307)
                     /locus_tag="POB55_001249"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HDI6500219.1"
                     /translation="MNKDLKGLYAALLVPFDENGQVNEQGLKQIAQNAIETEELDGLY
                     VNGSSGENFLLNTEQKKQVFKVAKEAVGDKVKLIAQVGSLDLNEAIELGKYATELGYD
                     ALSAVTPFYYPFTFEEIRDYYFDIIEATQNNMIIYAIPDLTGVNISIEQFSELFNHEK
                     IVGVKYTAPNFFLLERIRKAFPDKLILSGFDEMLVQATISGVDGAIGSTYNVNGRRAR
                     KIFDLARQGQIQEAYQLQHDSNDIIETVLSMGIYPTLKEILRHRGIDAGLPKRPFKPF
                     NEAHRQTLDQLIAKYDL"
     gene            10465..11325
                     /locus_tag="POB55_001250"
     CDS             10465..11325
                     /locus_tag="POB55_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDI6500220.1"
                     /translation="MYYIAIDIGGTQIKSAVIDKQLNMFDYQQISTPDNKSELITDKV
                     YEIVTGYMKQYQLIQPVIGISSAGVVDEQKGEIVYAGPTIPNYKGTNFKRLLKSLSPY
                     VKVKNDVNAALLGELKLHQYQAERIFCMTLGTGIGGAYKNNQGHIDNGELHKANEVGY
                     LLYRPTENTTFEQRAATSALKKRMIAGGFTRSTHVPVLFEAAEEGDDIAKQILNEWAE
                     DVAEGIAQIQVMYDPGLILIGGGISEQGDNLIKYIEPKVAHYLPKDYVYAPIQTTKSK
                     NDAALYGCLQ"
     gene            complement(11602..12402)
                     /locus_tag="POB55_001251"
     CDS             complement(11602..12402)
                     /locus_tag="POB55_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDI6500221.1"
                     /translation="MKFENRVQRYQHLFTKTDKRIVNYIRQNGYSDAFSTINSLTHAI
                     GTSPATMTRFSHKLDYENFQDLKFNIQQEMTETVIENSPIIQRIHKYHQQIIQQTGEF
                     IDNDIIQTFIDKLQSSRHILFAGLGSSGLSATEFYYRMIRMGLKGNVTTDSHLMKISA
                     SLLSHSDMFIAMSNSGNTSELISAAEVAKSHGAYVVAITNFEGSKLTDCADLVLLTTD
                     QSRNTDHQFINTQIATLFLIDIVSYHLLENTNLSQTYQHTKSIILDNK"
     gene            12543..13211
                     /locus_tag="POB55_001252"
     CDS             12543..13211
                     /locus_tag="POB55_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDI6500222.1"
                     /translation="MLPHGLIVSCQALPDEPLHSSFIMSKMALAAYEGGAVGIRANTK
                     EDILAIKETVDLPVIGIVKRDYNHSDVFITATSKEVDELIESQCEVIALDATLQQRPK
                     ETLDELVSYIRTHAPNVEIMADIATVEEAKNAARLGFDYIGTTLHGYTSYTQGQLLYQ
                     NDFQFLKDVLQSVDAKVIAEGNVITPDMYKRVMDLGVHCSVVGGAITRPKEITKRFVQ
                     VMED"
     gene            complement(13342..14655)
                     /locus_tag="POB55_001253"
     CDS             complement(13342..14655)
                     /locus_tag="POB55_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HDI6500223.1"
                     /translation="MNQYHSNAQQPSAWRFFVYSLVGILCFFIPFTINGNNTIFVDHV
                     HLAIRSIIGPLMPYVALIMILIGTALPIVRRTFMTSITNLVITLFKVAGAMIGIMYVF
                     KIGPSILFKANYGPFLFEKLMMPLSILIPVGAIALSLLVGYGLLEFVGVYMEPIMRPI
                     FKTPGKSAVDAVASFVGSYSLGLLITNRVYKQGMYNKREATIIATGFPTVSATFMIIV
                     AKTLGLMPHWNLYFWITLVITFVVTAITAWLPPITNESTEYYNGQEGEQEVAIEGSRL
                     KTAYAEAMKQNALTPSLVKNVWDNLKDGLEMTVGILPSILSIGFLGLIVANYTPFIDW
                     LGYIFYPFIYIFPIADQALLAKASAISIVEMFLPSLLVTKAAMSTKFVVGVVSVSAII
                     FFSALVPCILATEIKIPVWKLIIIWFLRVALSLLITIPVALLIFG"
BASE COUNT         5158 a         2290 c         2841 g         4636 t
ORIGIN      
        1 ttttaactgt attttaaata ttaatcatga ggtgataaga tgaataaaat ttcaaagtat
       61 attgcaatag catcattatc ggtagcggtt acagtttcag caccacaaac gacaaattct
      121 acagcgtttg ccaaaagttc tgctgaagtt caacaaacgc aacaagcttc tataccagca
      181 tcacaaaagg tgaatcttgg taatcaaaat attatggcag tggcttggta tcaaaattca
      241 gctgaagcaa aagcattata tttacaaggt tataacagtg cgaaagttca actagataaa
      301 gagattaaaa agaataaagg taaacataag ttagctattg ctttggattt agatgaaaca
      361 gttttagata attctccata tcaaggctat gcatcaatac ataataaacc tttcccagaa
      421 ggttggcatg aatgggtaca agctgctaaa gctaaacctg tctatggcgc aaaagaattc
      481 ttgaaatatg ctgacaaaaa agatgtcgat atctactata tttctgatag agataaagaa
      541 aaagatttaa aggcaacaca aaagaactta aaacaacaag gtatccctca agctaagaag
      601 agtcatattt tactaaaagg taaagatgat aagagtaaag aatcacgcag acaaatggtt
      661 caaaaggatc ataaacttgt catgctattt ggagataatt tattagactt tacagatcca
      721 aaagaagcta cagctgaatc tcgtgaagca ttaattgaaa aacataaaga cgatttcggt
      781 aagaaatata tcattttccc taacccaatg tatggtagtt gggaagctac aatttacaac
      841 aataactata aagcaagtga caaagcaaaa gataaattac gtaaaaatgc tattaagcaa
      901 ttcgatccta aaacaggcga agttaaataa tatatgaatt ggacgtctac atgtacttaa
      961 aggtatatgt aggcgttttt aaattgatcc ttaaatttta aatgtaccgt acaaaaataa
     1021 tagacaatta attgcatttt ccataaattt gcttttaata taaaaagttc gggtataatg
     1081 aaggataagt gtatgtataa cattacagag cgcaacacga cgtacgtgca tctaagaata
     1141 taaacaatgt ttaaatcata gtagtaggga gagaaatgca tgtttttagc ttggaatgaa
     1201 atacggcgca acaaattgaa gtttggacta attattggtg tgttaacgat gattagttac
     1261 ttgctatttt tattatctgg attggcgaat ggtcttatca atatgaataa agaaggtatt
     1321 gataagtggc aagcagaggc cattgttcta aataaagatg ccaatcaaac tgtgcaacaa
     1381 tctgttttta acaagaaaga tattgaaaat aaatacaaga agcaagctac tttgaagcaa
     1441 acaggggaaa ttgtgtctaa tggccatcaa aaagacaatg ttttagtgtt cggtgttgaa
     1501 aagtcatcat ttttagttcc gagtttaata gaagggcata aagcgactaa agataatgaa
     1561 gtgttagctg atgaaacact taaaaataaa ggatttaaaa ttggcgacac attatcacta
     1621 tctcaatcag atgaaaaatt gcatatcgta ggttttacag aaagtgcaaa atataatgcg
     1681 tcaccagtca ttttcacgaa tgacgctacc attgccaaga tcaatcctag attgactgga
     1741 gataaaatta atgcagttgt tgtacgtgat acaaattgga aagacaaaaa attaaaccaa
     1801 gagcttgaag cggtaagtat taatgacttt attgaaaatt taccaggtta taaaccacag
     1861 aacttaacat taaactttat gatttcattc ttatttgtca tttcagctac agttataggc
     1921 attttcttat atgtcatgac attacaaaaa acaagtttat ttggcatatt aaaggctcaa
     1981 ggatttacga atggctattt agcgaacgta gttatttcgc agacgctcat attagcatta
     2041 tttggtacgg catttggctt actgttaaca ggcgttacag gtgcattttt acctgatgca
     2101 gtacctgtca aattcgatgt actaacatta ctcgtatttg caattgtgtt aatgattgtc
     2161 tctgtattag gaagtttatt ctccatttta acaattagaa aaatagatcc gttaaaggcg
     2221 attgggtagg aggtgtagca aatgttgaaa tttgaaaatg taacaaagtc atttaaagat
     2281 gggaatcgta acattgaagc ggttaaagat acaaattttg agataaataa aggtgatatt
     2341 atagcattgg ttggaccttc tggctctggt aaaagtacat ttctaactat ggcaggtgct
     2401 ttacaaacgc cgacatctgg gcacatttta atcaataacc aagatattac gacaatgaag
     2461 caaaaagcat tggcaaaagt tagaatgtct gaaataggtt ttattttaca agctacaaac
     2521 cttgtgccat ttttaacggt aaagcaacaa tttacattat tgaaaaagaa aaataagaat
     2581 gttatgtcta atgaagacta tcagcaactt atgtcacaat tagggctaac ttcattgctt
     2641 aataagttac cttcagaaat ttcaggtggt cagaaacaac gtgtggcgat agctaaagcg
     2701 ttatatacga atccgtcgat tattttagcg gatgaaccta ccgcggcgtt agatactgaa
     2761 aatgcgattg aagtcattaa aattctacgt gatcaagcca aacaaagaaa gaaagcatgt
     2821 attattgtta cacatgatga acgacttaaa gcatattgtg atcgttcata tcatatgaaa
     2881 gatggcgtcc ttaatcttga aaatgaaaca gtagaatagt tttattaagc cggtacatca
     2941 tgtgccggta tttttatgtt tatgtattat ttgaataaac tttcacattc aattaataat
     3001 aattattatc gaaaattaga aatattccgt gaaatataat attttttgta gtaaaatggc
     3061 ctctaagtat tcaatattta aatatgggga ttgaatataa aattatcgta atgggggtca
     3121 atggttatgg atttattgat aggtacttta tttttatttt tggtcttagt gatttttaca
     3181 ttatttacat ataaagcacc taatggtatg cgtgccatgg gagcattagc taatgcagca
     3241 atcgcaacat ttttagtaga agcatttaat aaatatgttg gtggcgaagt attcggtatt
     3301 aaatttttag aagagctagg agacgctgcg ggaggtctag gtggtgtcgc tgccgctgga
     3361 ttaacagcat tagctatcgg tgtgtcacca gtatatgcat tagttatagc agccgcgtgc
     3421 ggtggtatgg atttattacc aggtttcttt gcgggttata tgattggata tgtgatgaaa
     3481 tatacagaga aatatgtgcc ggatggtgtc gacttaattg gatcgattgt catcttagcg
     3541 ccattagctc gtcttattgc agtattatta acgccagtag tgaatagtac attgattcga
     3601 ataggtgata ttatccaaag tagtacgaat acgaatccaa ttatcatggg tatcatttta
     3661 ggtggtatta tcacggttgt cggtacagcg ccattgagtt caatggcatt gacagcatta
     3721 ttaggtttaa cgggtgtacc tatggctatt ggtgccatgg cagcatttag ttcggcattt
     3781 atgaatggga cgctattcca tcgcttaaaa ttaggtgatc gtaagtctac gattgcagta
     3841 agtattgaac ctttatcaca agcagatatt gtatcagcca atccaattcc aatctatatt
     3901 acaaatttct ttggtggtgc gattgctggt ttaattattg ctatgtcagg tttaattaac
     3961 gatgcgacag gtacagctac accgattgca ggatttttag ttatgtttgg atttaatcat
     4021 ccgacgacaa ttgtgattta tggtgtagta atggcgattg taggtgcgct tgcaggttat
     4081 cttggttcaa ttgtatttaa aaaatatcca attgttacta agcaagacat gattaatcga
     4141 ggtgcagtag acgcatagca tcatcatatt gaatagtaaa aacaaataaa acatagtaac
     4201 gtgattcagt cgatgtaaca gtcgataatg agtcacgttt ttttatagaa aaatataaga
     4261 cataaaaatg tcataattta ttgtcgacaa atatcatact gtataaacat ttatcatttt
     4321 ctcaagtacc ttttacacga tggaatgaac ttacttttta cgaaattatg cgtattttat
     4381 aaacaaatat cattgatata acggtaaatg taagcgttta caacagaaat aacagcatgc
     4441 tacgatattt ttgtgaattc actgattcaa gtattttaag tcaatatgag gagggatgtt
     4501 atgagcgatt ctgagaaaga aattttaaaa agaattaaag ataatccgtt tatttcacaa
     4561 cgtgaacttg ctgaggcaat tggattatct agacccagcg tagcaaacat tatttcagga
     4621 ttaatacaaa aggaatatgt tatgggaaag gcatatgttt taaatgaaga ttatcctatt
     4681 gtttgtattg gcgcagcgaa tgtagatcgt aagttttatg tgcataaaaa tttagttgca
     4741 gaaacatcaa atcctgtaac gtcaacacgc tctattggtg gcgtagcaag aaatattgct
     4801 gagaacttag gtaggcttgg cgaaacggtc gcttttttat ctgctagtgg acaagatagt
     4861 gaatgggaaa tgattaaacg attgtccaca ccatttatga atttggatca tgttcaacaa
     4921 tttgaaaatg cgagtacagg ttcatataca gctttaatta gtaaagaagg cgacatgaca
     4981 tatggcttag cagatatgga agtgtttgac tacattacgc ctgaattttt aattaagcgt
     5041 tcacacttat tgaaaaaggc taagtgcatt attgtcgatt tgaatttagg caaagaggca
     5101 ttaaacttct tatgtgccta taccacgaaa catcaaatca aattagttat caccacggtt
     5161 tcttctccaa aaatgaaaaa tatgcctgat tcattacatg ctattgattg gattatcacg
     5221 aataaagatg aaacagaaac atacttaaat ttaaaaatag aatctactga tgatttaaaa
     5281 atagctgcta aacgctggaa tgatttaggt gttaaaaatg ttattgtgac aaatggcgtg
     5341 aaagaactca tttatcgaag tggtgaggaa gaaatcatta agtcagttat gccatcaaat
     5401 agtgtgaaag atgttacagg tgcaggcgat tcattctgtg ctgcagtagt gtatagctgg
     5461 ttaaatggga tgtctactga agatatatta attgctggta tggttaacgc aaagaaaacg
     5521 atagaaacga aatatacagt taggcaaaac ctagatcaac agcaacttta tcacgatatg
     5581 gaggattata aaaatggcaa atttacaaaa gtatattgag tattctcgag aagttcagca
     5641 agcactggag aacaatcaac cgattgtagc attagaatca acaattatat cgcatggtat
     5701 gccgtaccca caaaatgttg aaatggcaac aacagtagag caaattatca ggaataatgg
     5761 tgccattcca gcaaccatag ccattataga tggcaaaatt aaaattggtt tagaaagcga
     5821 agatttagaa atactggcaa ctagtaaaga cgttgctaaa gtatctagaa gggatttagc
     5881 agaagttgtt gcgatgaagt gtgttggtgc tactactgta gcgacgacga tgatatgtgc
     5941 tgcaatggct ggtattcaat tttttgttac aggaggtatt gggggcgtcc ataaaggtgc
     6001 agaacatacg atggacattt cagcagactt agaagaactg tctaaaacaa atgtcactgt
     6061 tatctgtgca ggtgccaaat caattttaga cttacctaag acgatggagt atttagaaac
     6121 aaaaggcgtt ccagttattg gatatcaaac gaatgaattg ccagcattct tcactcgcga
     6181 aagcggtgtt aagttaacaa gttcggttga aacgccagaa cgacttgctg acattcattt
     6241 aacaaaacag cagttaaatc ttgaaggtgg cattgttgtt gctaatccaa ttccatatga
     6301 gcatgcctta tcaaaagcat atattgaggc aatcataaat gaagctgttg ttgaagcgga
     6361 aaatcaaggt attaaaggta aggacgccac accgttcttg ttagggaaaa ttgtagaaaa
     6421 aacgaatggt aaaagtttag cagcaaatat aaaacttgtt gaaaacaatg cggcgttggg
     6481 tgctaaaatt gctgtcgctg ttaataaatt attgtaggtg atgatacatg aatattttat
     6541 tcgctatcac agggatagca tttgcactat ttgttgcgtt tttattcagt tttgatcgta
     6601 aaaacataga cttcaaaaag acgttaataa tgatatttat tcaagtgttg attgtgttat
     6661 ttatgatgaa cacaacgatt ggtttaacaa tcttaactgc attaggctca ttttttgaag
     6721 ggttaataaa tgttagtaaa gcaggtataa actttgtttt tggagatata caaaataaaa
     6781 atggctttac gttcttttta aatgtgttgc tgccattagt gttcatttct gtattaatag
     6841 gcatttttaa ttatattaag gtattaccat ttattatcaa atatgtaggt atcgctatta
     6901 ataaaataac gagaatgggg cgcttagaaa gttactttgc tatttcaaca gcaatgtttg
     6961 gacaaccaga agtatattta acaataaaag atattattcc gagattatct agagcgaaat
     7021 tatatacaat tgcgacgtct ggtatgagtg ctgttagtat ggcaatgttg ggttcatata
     7081 tgcagatgat tgagcccaag ttcgtagtta cagctgtaat gttaaatatt tttagtgcgc
     7141 ttatcatcgc cagtgtaatc aatccctata aatctgatga tagtgatgtt gaaattgata
     7201 acttaactaa atcaacggaa acgaaatcag tgaatggaaa aacaggaaaa cctaagaaag
     7261 ttgccttttt ccaaatgatt ggtgatagtg cgatggatgg gtttaaaatc gctgttgtag
     7321 tagccgtaat gttgttagca tttatttcat taatggaagc aattaatatc atgtttggta
     7381 gtgttggttt gaactttaaa cagcttattg gctatgtgtt tgcaccaatc gcattcttaa
     7441 tggggattcc atggagcgaa gctgttccag ctggctcttt aatggcgact aaattaatta
     7501 caaatgagtt tgtagcaatg cttgatttta aaaatgtcct gggtgatgta tcagctcgaa
     7561 cacaaggtat catttcagtt tacttagtaa gcttcgctaa ttttggtacg gttggtatca
     7621 tcgtaggttc aattaaaggc attagtgata aacaaggaga aaaagttgca tcctttgcaa
     7681 tgaggttgct acttggttca actctagctt ctatcatttc aggatcaatc attggcctag
     7741 tattataatt taatcgaagt acctaaatta aattcataac aacatcaaaa ccccgtcacc
     7801 aagttggcgc aacagcgcat cataacttag tgacggggtt ttatcataac aatctacttt
     7861 ttcgtagcca tttttgaaat gtatgttgat ggtttatctt tttcaaaaat tgttaatccc
     7921 gttatatctt ttttatgttt tgaagggaca atgaagctaa gtatataagc aaagacaaaa
     7981 gcaactgtaa atgaaatggt agatacatag aaaggtgagt tacctttgcc aacaccatta
     8041 tagacataag caaagatgat acccaatatt aatccacaaa taacaccgaa tgtattcgta
     8101 cgtttagtga aaataccaac tgcaaataca ccagccaatg gaacgccgaa taatccagtc
     8161 acaaacaaga ataaatccca taagtcattt gaattagaag caattaagta tagtgacatt
     8221 ccaaaaccga aaatacctgc aatgatgata atgaaacgtg caaagttaac ttcgtgtcgc
     8281 tcgctacctt ttccgaagaa gcgttgctta atgtcgattg aaatacaagc agatatagaa
     8341 tttaaactag atgaaatggt agactgtgca gcggcgaaaa tggctgcaat aagtaatcct
     8401 gctacaaatg gtggcatctc agtcaaaatg aaatatggca ctacagatga tgtattgaag
     8461 ccttttggta aaacagcttc atgtgtataa aatgaataca gcattgtacc cataccataa
     8521 aataaggggg ctgaaattaa agctaggata ccatttgtcc ataacgattt atttgtttct
     8581 tttaaactat cagaagcttg ataacgctgc acgacgtctt gactcgctgt gtattgatac
     8641 aagttgttga aaatatttcc taggaaaata attggaatgg cagctgccgc agtatttagt
     8701 ttccaattgt ctgcactaat taattttttg tgctcaatcg catctgcaaa gacagtgcca
     8761 aaaccgcctt taatgttcat aacacctaga ataatgataa ctaaagcgcc gcctaataaa
     8821 atgacgcctt gaatgaaatc actccaaacc acaccttcga atccacctaa aaatgtatat
     8881 aaaatacata gtaaaccaac gagtgatgca acgatataag ggttcatgtc tgatacagat
     8941 gtgattgcta atgttggtaa gtagataacg attgcaacac gccctaaatg gtaaacgaca
     9001 aataataatg agccaataac acgtatgcta gggccaaatc tagcttctaa atattcatat
     9061 gctgatgtta cttttaactt tttaaagaaa gggacataga aataaatgag taatggaata
     9121 attgcgacga tagcaatgtt accagcgata tatgaccaat ctgttaaaaa tgctttctct
     9181 ggtgtcgaca taaatgtaat cgcacttaac gtagtagcat aaattgaaaa gccaactacc
     9241 caagatggca agcgaccact tgcggtaaag aaactattgg tactttggct cgcgcgcttg
     9301 gtaaaataaa cgccaatgaa caacatagct agtagataaa tgataacggc aacccagttt
     9361 agtgtgccaa atccaatttc tttcatgggc aacatcccct ttacaatgta ttgattcttt
     9421 gatgtctata aatcgtattt tgcaatgagt tgatctaatg tttgtcgatg tgcttcgtta
     9481 aaaggtttga aaggtctttt cggtaatcct gcatcaatgc cacgatgacg taatatttct
     9541 ttcaatgttg gataaatccc cattgataac actgtttcga taatgtcgtt tgaatcatgt
     9601 tgtagttggt aagcttcttg aatttgacct tgtcttgcta agtcgaagat ttttctagcg
     9661 cggcgaccat taacgttata tgtagaacca attgcaccat ctacgccaga aatcgtagct
     9721 tgaactaaca tttcatcaaa gccagataag attaatttgt ctgggaatgc ttttctaata
     9781 cgttcgagta ggaagaagtt tggcgctgta tatttaacac caacaatttt ttcatgatta
     9841 aatagctcgc tgaattgttc aatagaaata ttcacacctg ttaaatctgg tattgcataa
     9901 ataatcatat tgttctgagt tgcttcgata atatcgaaat agtaatctct aatttcttca
     9961 aaagtaaatg gatagtagaa tggtgttacg gcagaaagtg catcataacc gagttctgtg
    10021 gcatattttc caagttcaat ggcttcattt aaatctaacg aacctacttg agcaatcaat
    10081 ttcactttat ccccaactgc ctctttggca actttgaaaa cttgcttctt ctgctctgta
    10141 tttaataaaa agttttcgcc tgagctacca tttacataaa gaccgtctaa ttcttcagtt
    10201 tcaatggcat tttgagcaat ttgtttgagt ccttgttcat ttacttgacc attttcatca
    10261 aaaggaacga gtaacgctgc atataaacct tttaaatctt tgttcattat gaagtccctc
    10321 caaaaatcat ttgataatat agtttacagc tataattgta aacgctatca taaaatgtaa
    10381 caatatcttt ttgaaaattg tagtcatatt tatgtataat taatgaaaat gtttttcaaa
    10441 atcaatagaa atggagtgag taaggtgtat tacatcgcaa tcgatattgg aggcactcaa
    10501 attaaatcgg cagttattga taagcaattg aatatgtttg actatcaaca aatatcaacg
    10561 ccggacaaca aaagtgagct tattactgac aaagtatatg agattgtaac aggatatatg
    10621 aagcaatatc agttgatcca acctgtcata ggtatttcat cagcaggcgt tgttgatgaa
    10681 caaaaaggcg aaattgtata cgcagggcca accattccga attataaagg tactaatttt
    10741 aagcgattat taaaatcact gtctccttat gtcaaagtaa aaaatgatgt aaacgctgca
    10801 ttactaggcg aattgaaatt acatcaatat caagcagaac ggatcttttg tatgacgctt
    10861 ggtacaggca ttgggggtgc gtacaagaat aatcaaggtc atattgataa tggtgagctt
    10921 cataaggcaa atgaagttgg gtatttattg tatcgtccaa ctgaaaatac aacgtttgag
    10981 caacgtgctg caacgagtgc attgaaaaag cgcatgattg ccggaggatt tacgagaagc
    11041 acacatgtgc cagtattgtt tgaagcagct gaagaaggtg atgatattgc aaaacaaata
    11101 ttgaatgagt gggcagaaga tgtagcagaa gggattgccc aaatacaggt catgtatgat
    11161 ccagggctta tattaattgg gggcggtata tctgaacaag gagataatct cattaaatat
    11221 atcgagccga aagttgcaca ctatttacca aaagactatg tttatgcacc aatacaaacg
    11281 actaagagta aaaatgatgc agcattatat ggctgtttgc aatgatagtt gaaagaagga
    11341 gtcattctaa aatagaattt gaaaccgtta cgagagatga gagctgttgt tagttccaca
    11401 catcacactc tatctaggac caatctaaac tatatcaacc aacagtgtgc cacagacata
    11461 ttaaattgaa gaagctgaga tattaaaatt ttagaaaatg aaaaaaatat ttggtattga
    11521 aattaaaaaa gcacctagca actcgttggg acaatcacga tgattgtcta cagttgcagg
    11581 tggatttgaa tatactacta gttatttgtt gtctaggata atagatttag tatgttgata
    11641 agtttgactc agattcgtat tttctaataa atgatagctc acgatatcga ttaaaaagag
    11701 tgtcgcaatt tgtgtgttga taaattgatg gtcggtatta cgcgattgat ccgttgttaa
    11761 aagtactaaa tctgcacaat ctgtaagttt gctaccttcg aaatttgtga tggcaacgac
    11821 atatgcacca tgagatttgg cgacttccgc tgcagaaatt aattccgaag tattaccact
    11881 atttgacata gcaataaaca tatccgaatg agatagtagg gatgccgata ttttcattaa
    11941 atgtgaatcg gtagtaacat taccttttag ccccatacga atcatacgat aataaaattc
    12001 agtcgctgat aaaccagagc tacctagtcc agcaaagagt atatgtcgac ttgattgaag
    12061 tttgtcgata aaggtttgga taatgtcgtt atcaataaat tcaccagttt gttgaatgat
    12121 ttgttgatga tatttatgaa ttctttgaat aattgggcta ttttcaataa ctgtctctgt
    12181 catttcttgt tgaatattaa attttaaatc ttggaaattc tcataatcca gcttatgact
    12241 aaagcgtgtc atcgttgctg gtgatgtacc aatcgcatgg gttaaggagt taatcgttga
    12301 aaaggcatcg ctataaccat tttgtcttat ataattgacg atgcgtttat cagtttttgt
    12361 aaataaatgt tgataacgtt gaacacgatt ctcaaatttc attgtgtcac cccttcatct
    12421 taatgattac tattatatat gaaaaatatt ttcaagatag taaaaagcat tgataaaaat
    12481 tatcttaatg atatattgta aatgacttta cgtgaaaaaa acgacttatg gagtgaggaa
    12541 taatgttacc acatggatta atagtatctt gtcaggcact accagatgaa ccattgcatt
    12601 catcttttat tatgtcgaaa atggcattag ctgcgtatga aggtggtgct gttggtattc
    12661 gcgcaaatac taaggaagac attttagcaa ttaaagaaac ggtagattta ccagttattg
    12721 gcattgtgaa acgtgactat aatcactcag atgttttcat tacagcaacg tcaaaagaag
    12781 ttgatgaact gatagaaagc caatgtgaag tcattgcatt ggatgcaacg ttacagcaac
    12841 gtccaaaaga aacgttagac gaattagtat catatattag aacacatgca ccgaacgttg
    12901 aaatcatggc tgatatcgcg accgttgaag aagctaaaaa tgccgcacga cttggctttg
    12961 attatattgg cacgacgtta catggctata ctagttatac gcaaggacaa ttactttatc
    13021 aaaatgactt ccaatttcta aaagatgtac tacaaagtgt tgatgcaaaa gttattgcgg
    13081 aaggtaatgt cattacaccg gatatgtata aacgcgtgat ggacttaggc gttcattgtt
    13141 cagtcgttgg tggtgcgata acacgaccaa aagaaattac gaaacgtttt gttcaagtta
    13201 tggaagatta aatgataacg ataaaaaaac gagatgacca tcattaatta aaggtaccta
    13261 attatcttag gtggctgaat gaatgtaatg ggttcatctc gttttgtttg tttacgatag
    13321 tgattttatt ttcaatttga attatccaaa aataagtaaa gcgacgggga tggtgattaa
    13381 tagcgacaat gccacgcgta aaaaccaaat gatgatgagt ttccagacag gtattttaat
    13441 ttcagttgct agtatacatg gcactaatgc tgagaaaaag ataatggctg atacgcttac
    13501 tacaccgacg acaaatttag tactcattgc agctttagtt actaacaaag atggtagaaa
    13561 catctctaca atagaaatcg ctgacgcttt tgcaagtaaa gcctgatcag caattgggaa
    13621 aatataaata aatggataga agatatagcc aagccaatca atgaatggtg tatagttcgc
    13681 tacaatcagt cctaaaaaac caatcgataa tatagaaggc aaaataccaa cagtcatttc
    13741 taaaccgtct ttcaaattgt cccaaacgtt cttcacgagg gatggtgtta atgcattttg
    13801 tttcatcgcc tctgcatatg cagttttcag tctgcttcct tcaatagcaa cttcttgttc
    13861 tccttcttgt ccgttataat attctgttga ttcattggtg attggcggta gccatgcagt
    13921 aattgcagtc acgacaaatg tgatgactaa agttatccaa aagtataaat tccaatgcgg
    13981 cattaatcct aaagttttag caacgataat cataaaagtt gctgaaactg ttggaaagcc
    14041 agtcgcaata atcgtggctt ctcgtttgtt gtacatccct tgcttataga cacgattagt
    14101 aatcaataat cctaaggaat aactgccgac aaacgaagct actgcatcga cagcggattt
    14161 tcctggtgtt ttaaaaatag gtctcataat aggctccata taaacaccga caaattctaa
    14221 taagccatag cctactaata aagaaagcgc aattgcacct actggaatta agatacttaa
    14281 tggcatcatt aatttttcaa acaagaacgg accatagtta gctttaaata gtattgatgg
    14341 accgatttta aatacataca ttataccaat cattgcacct gcaactttaa ataatgtaat
    14401 gaccaagttt gtgattgaag tcataaaagt acgtctcact attggtaacg ctgtaccaat
    14461 taaaatcata atcagtgcaa catagggcat aagtggacct atgattgagc gaatggctag
    14521 atgaacatga tcgacgaaaa tagtgttgtt accattaatc gtaaaaggaa taaagaaaca
    14581 tagtatgccc actaaactat agacaaaaaa acgccatgca cttggttgtt gtgcattaga
    14641 atgatattga ttcattaaag caaccccttt gtttaaatga atacacaaaa ctgtatgatg
    14701 catcttcccc ttaatgagat gaatcattat tttaatttag aaaaatctga aaacttatta
    14761 taattgtata gtttgaatta ttttcagacc aatacaaatt aactaattat atatagattg
    14821 aaactatatt acttaataaa atatttatct taaatgttgt tgtgatgatt caacgccaca
    14881 actaaaagtg tttataaatt atttggaaat gcacatattt gtaaa
//
LOCUS       DAMZKC010000055        14725 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.055, whole genome shotgun sequence.
ACCESSION   DAMZKC010000055 DAMZKC010000000
VERSION     DAMZKC010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14725
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.055"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            51..842
                     /locus_tag="POB55_001254"
     CDS             51..842
                     /locus_tag="POB55_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDI6500224.1"
                     /translation="MTHRLNKGGNFTLNKHERLDEIAKLVNKKGTIRTNEIVEGLNVS
                     DMTVRRDLIELENKGILTKIHGGARSNSTFQYKEISHKEKHTRQIAEKRFIAKKAASL
                     IEDGDTLFFGPGTTVELLAEEVNHHTLTIITNCLPVYKILLEKQTAHFRVYLIGGEMR
                     HITEAFVGEMANAMLEKLRFSKMFFSSNAVNKGAVMTSTLDEAYTQQLALSNSIEKYL
                     LIDHTKVGKEDFTSFCQLNELTAVVMDYEDEEKVETIKTYIEVVD"
     gene            1210..1638
                     /gene="lacA"
                     /locus_tag="POB55_001255"
     CDS             1210..1638
                     /gene="lacA"
                     /locus_tag="POB55_001255"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HDI6500225.1"
                     /translation="MAIIIGSDEAGKRLKEVIKSYLLDNKYDVVDVTEGQEVDFVDAT
                     LAVAKDVQSQEGNLGIVIDAFGAGSFMVATKIKGMIAAEVSDERSGYMTRGHNNSRMI
                     TMGSEIVGDTLAKNVVKGFVEGKYDGGRHQIRVDMLNKMC"
     gene            1654..2169
                     /gene="lacB"
                     /locus_tag="POB55_001256"
     CDS             1654..2169
                     /gene="lacB"
                     /locus_tag="POB55_001256"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HDI6500226.1"
                     /translation="MKIALGCDHIVTDTKMRVSEFLKSKGHEVIDVGTYDFTRTHYPI
                     FGKKVGEQVVSGNADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSALYAKEELNAN
                     VIGFGGRIIGELLMCDIIDAFINAEYKPTEENKKLIAKIKHLETSNADQADPHFFDEF
                     LEKWDRGEYHD"
     gene            2182..3114
                     /gene="lacC"
                     /locus_tag="POB55_001257"
     CDS             2182..3114
                     /gene="lacC"
                     /locus_tag="POB55_001257"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HDI6500227.1"
                     /translation="MILTLTLNPSVDISYPLTALKLDDVNRVQEVSKTAGGKGLNVTR
                     VLAQVGEPVLASGFIGGELGQFIAKKLDHADIKHAFYNIKGETRNCIAILHEGQQTEI
                     LEQGPEIDNQEAAGFIKHFEQLLEKVEAVAISGSLPKGLNQDYYAQIIERCQNKGVPV
                     ILDCSGATLQTVLENPYKPTVIKPNISELYQLLNQPLDESLESLKQAVSQPLFEGIEW
                     IIVSLGAQGAFAKHNHTFYRVNIPTISVLNPVGSGDSTVAGITSAILNHENDHDLLKK
                     ANTLGMLNAQEAQTGYVNLNNYDDLFNQIEVLEV"
     gene            3118..4098
                     /gene="lacD"
                     /locus_tag="POB55_001258"
     CDS             3118..4098
                     /gene="lacD"
                     /locus_tag="POB55_001258"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HDI6500228.1"
                     /translation="MSKSNQKIASIEQLSNNEGIISALAFDQRGALKRMMAKHQTEEP
                     TVAQIEQLKVLVAEELTQYASSILLDPEYGLPASDARNKDCGLLLAYEKTGYDVNAKG
                     RLPDCLVEWSAKRLKEQGANAVKFLLYYDVDDAEEINIQKKAYIERIGSECVAEDIPF
                     FLEVLTYDDNIPDNGSVEFAKVKPRKVNEAMKLFSEPRFNVDVLKVEVPVNMKYVEGF
                     AEGEVVYTKEEAAQHFKNQDAATHLPYIYLSAGVSAELFQETLKFAHEAGAKFNGVLC
                     GRATWSGAVQVYIEQGEDAAREWLRTTGFKNIDDLNKVLKDTATSWKQRK"
     gene            4120..4431
                     /locus_tag="POB55_001259"
     CDS             4120..4431
                     /locus_tag="POB55_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDI6500229.1"
                     /translation="MNREEVQLLGFEIVAFAGDARSKFLEALTAAQAGDFAKADALIE
                     EGNNCIAEAHRAQTSLLAKEAQGDDIAYSVTMMHGQDHLMTTILLKDLMKHLLEFYKR
                     G"
     gene            4437..6149
                     /locus_tag="POB55_001260"
     CDS             4437..6149
                     /locus_tag="POB55_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDI6500230.1"
                     /translation="MMQKLIAQIEKGKPFFEKLSRNIYLRAIRDGFISAMPVILFSSI
                     FLLIAYVPNIFGFKWDKGMEAILMKPYNYTMGLVAFLVAGTTAKSLTDSFNRKLESTN
                     QINFISTMLAAMCGFLFLASDPAKDGGFLSAFMGTKGLLTAFLSAFVTVIVYNFCVKR
                     NITIKMPKEVPPNISQVFKDLIPFSAVIIILYALDLVIRNSFKSNVAEGILKLFEPLF
                     TAADGWIGVTIIFGAFALFWFVGIHGPSIVEPAIAAITYANIEANFKLLQAGEHADKI
                     ITSGTQMFIVTFGGTGATLVVPFMFMWMTKSKRNKAIGRASVVPTFFGVNEPILFGAP
                     LVLNPVFFIPFVLAPIVNVWIFKLFVEVLGMNSFSVNLPWTTPGPLGIIMGTGFGLWS
                     FVLAITLIVVDIIIYYPFLKVYDSEILDEEEGRKESNSDLKEKVAANFDTKKADSILA
                     ASGVSDDAAKASNITEQTNVLVLCAGGGTSGLLANALNKAAEEYHVPVKAAAGGYGAH
                     MDIMKEYQLIILAPQVASNYEDIKQDTDRLGIKLAKTQGAEYIKLTRDGQAALDFVQQ
                     QFEN"
     gene            6167..7579
                     /gene="lacG"
                     /locus_tag="POB55_001261"
     CDS             6167..7579
                     /gene="lacG"
                     /locus_tag="POB55_001261"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HDI6500231.1"
                     /translation="MTKTLPEDFIFGGATAAYQAEGATNTDGKGRVAWDTYLEENYWY
                     TAEPASDFYNRYPVDLELSEKFGVNGIRISIAWSRIFPNGYGEVNPKGVEYYHKLFAE
                     CHKRHVEPFVTLHHFDTPEVLHKDGDFLNRKTIDYFVDYAEYCFKEFPEVKYWTTFNE
                     IGPIGDGQYLVGKFPPGIKYDFEKVFQSHHNMMVAHARAVKLFKDGGYQGEIGVVHAL
                     PTKYPFDPSNPEDVRAAELEDIIHNKFILDATYLGKYSRETMEGVQHILSVNGGKLNI
                     TDEDYAILDAAKDLNDFLGINYYMSDWMRGYDGESEITHNATGDKGGSKYQLKGVGQR
                     EFDVDVPRTDWDWMIYPQGLYDQIMRVVKDYPNYHKIYITENGLGYKDEFIESEKTVH
                     DDARIDYVRQHLNVIADAIKDGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQE
                     RYPKKSAYWYKELAETKEIK"
     gene            7845..8714
                     /locus_tag="POB55_001262"
     CDS             7845..8714
                     /locus_tag="POB55_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDI6500232.1"
                     /translation="MKKIFVIITTLLAVAIIIGSIITVVFSQRQAQTFKIQQQQFVKK
                     PIPTLFLHGFGGSANSEKFMVKQAEKRGVTKDIITAYVSKDGAVTFKGKLRKDAVNPI
                     VKIELENNRQGYLDKNAAWFKNVLTKLQSEYNFDKFNFVGHSMGNLTFAQYMMTYGND
                     KSLPQLNKQVNIAGTFNGVLNMNEDVNEITVDKDGKPSRMNQPYQQLRVLKDIYKGKG
                     IEVLNIYGDLKDGTHSDGRVSNSSSKSLKYLLGNSPKSYRESKYEGEPAQHSQLHENE
                     NVANELIDFLWKK"
     gene            8997..9998
                     /locus_tag="POB55_001263"
     CDS             8997..9998
                     /locus_tag="POB55_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HDI6500233.1"
                     /translation="MQNKQILFNKIPEGMPQEDTFKIEETDTPKLESDGVLVQTLYIS
                     VDPYMRGRMTKADSYVQPFEIGKPIVSHVVAKVIDSTLADYKKGDVVVGMLPWRIINH
                     VQADQITKVPTTDVPLDLYLSVLGMPGQTAYHGLLDIGQPKAGDTVVVSAASGAVGSV
                     VGQIAKIKGCRVVGIAGGDKKVNYLTGTLGFDAGIDYKKEDFAEALAQAVPDGIDVYF
                     ENVGGTVGDEVFKHLNRFARVPVCGAISSYNHPEADIGPRIQGTLIKKQVMMRGFLVS
                     EFANAFKEASEQLATWVQEGKIQSQVTIEDGFENAPHAFKNLFTGDNFGKQVIKVTE"
     gene            10232..11239
                     /locus_tag="POB55_001264"
     CDS             10232..11239
                     /locus_tag="POB55_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HDI6500234.1"
                     /translation="MKMIGFEKPFKLEEGNLFKVYEQRKPTPENDDILVKVNSISVNP
                     VDTKQRQMEVTQAPRVLGFDAIGTVEAIGPDVTLFSPGDVVFYAGSPNRQGSNATYQL
                     VSEAIVAKAPHNISANEAVSLPLTGITAYETFFDTFKISHNPAENEGKSVLIINGAGG
                     VGSIATQIAKRYGLTVITTASRQETTEWCEKMGADIVLNHKEDLVRQFKEKEIPLVDY
                     IFCTYNTDLYYNTMIELIKPLGHITTIVAFNEDQDLNALKLKSITFTHEFMFARPIHR
                     TPDMIKQHEYLEDITKNIESGHYQPTTTQVFEGLSPENLYQAHQLLEKQSMIGKLVIN
                     I"
     gene            11547..13109
                     /locus_tag="POB55_001265"
     CDS             11547..13109
                     /locus_tag="POB55_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HDI6500235.1"
                     /translation="MKNKSVSLVFWVSLVICTIFVAFGAIFPKQLEKLTQNITTFIAL
                     HFSWYYLLLVLVILFVCVYILFSRYANITLGEEGEDPEFSLPSWFAMLFSAGMGIGLV
                     FWTTAEPISHAFKLTPIHKAGTQSAINDAMQFSFFHWGIHAWAVYGIVALVFAYFSFH
                     KGYPGLVSATLTPLLGEKAMRGPLGGAIDVLAVIATVTGVAATLGFGALQINEGLHFL
                     FNVPSNFTMQVILIVIATILFTWSAWSGIDKGIKTLSNINMLLAFVVLIGLFIVGPTL
                     YILNTFTNGLGNYIANFFSMSLRIPSGGQKFQWLQNWTIFYWAWWISWAPFVGIFIAR
                     VSKGRTIKEFILGVLFVPALVCFIFFAVFGASAIYLQDNHIADIAKAATETATFATLQ
                     HYPLGFVLSLITLLVIMIFFVTSADSATYVLGMLSSRGDINPKSFVKVSWGIIMALFA
                     IIMIYTGGTQAIQNLLIIAALPFSVVIIAMIWSLLKSLSEEKPRNSNKVLIKHRDPDI
                     LEYRSSKHIDEN"
     gene            13284..13820
                     /gene="amaP"
                     /locus_tag="POB55_001266"
     CDS             13284..13820
                     /gene="amaP"
                     /locus_tag="POB55_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HDI6500236.1"
                     /translation="MKRLKNFILGLLIVVIVGFLLFMYIQDGRITEYQDYFLQFEWFQ
                     PLLISLAALLILIGLILVFSIFKPTHRKPGLYKDFDDGHVYVSRKAVEKTAFDTVAKY
                     DQVRQPNVVAKLYNKKNKSYIDIKTDFFVPNNVQVQSLTEAIRSDIKKNVEYFTEMPV
                     RKLEVNVRDQKTSGPRVL"
     gene            13833..14072
                     /locus_tag="POB55_001267"
     CDS             13833..14072
                     /locus_tag="POB55_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HDI6500237.1"
                     /translation="MANNHNQNGQDSTQQVINFLKVFKWRIVGFLAFLLIAILFLTLG
                     FWKTVLIIVLCLIGVGIGYMKDRKQDFMNFLNRWS"
     gene            14135..14644
                     /locus_tag="POB55_001268"
     CDS             14135..14644
                     /locus_tag="POB55_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDI6500238.1"
                     /translation="MTVDNNKAKQAYDNQTGVNEKEREERQKQQEQNQEPQFKNKLTF
                     SDEVVEKIAGIAAREVKGILDMKGGLTDTFTNAFSSGNNVTQGVSVEVGEKQAAVDLK
                     VILEYGESAPKIFRKVTELVKEQVKYITGLDVVEVNMQVDDVMTQKEWKQKHEKNNEN
                     NNQERQGLQ"
BASE COUNT         5186 a         2109 c         2882 g         4548 t
ORIGIN      
        1 attatagaat aagcatgtat attaaaattg atgtctaaac ataaaggata atgacccatc
       61 gcctaaataa aggaggcaat tttacattga ataaacatga acgtttggac gaaattgcta
      121 aactagtgaa taaaaagggc acgataagaa cgaatgaaat cgtcgaaggt ttaaatgtgt
      181 ctgatatgac agttcgaaga gatttaattg agttggaaaa taaagggatt ttaacgaaga
      241 ttcatggtgg tgcacgcagt aattcaacat ttcagtataa agaaatatcg cataaagaaa
      301 aacatacccg tcaaattgca gaaaaacgat ttatagctaa aaaagctgca tcattaattg
      361 aagatgggga tactttgttt ttcggaccgg gaacaacagt ggaactatta gcggaagaag
      421 tcaatcatca tacgctcaca attattacga attgtttgcc agtgtataaa attttgttgg
      481 aaaaacaaac agcacatttt cgtgtctatt taattggtgg tgaaatgcgc catattacag
      541 aagcgtttgt aggtgaaatg gcgaatgcca tgttagaaaa actaagattt agcaagatgt
      601 tctttagtag taacgcagta aataaaggtg ctgtgatgac atctacatta gatgaagcct
      661 atacgcaaca attggcacta agtaattcaa ttgaaaaata cttgttaatc gatcatacga
      721 aagttggcaa agaagatttt acatcatttt gtcagttaaa cgaattgact gcagtggtca
      781 tggactatga agatgaagaa aaagtagaaa cgattaaaac atatattgaa gtagtcgact
      841 aaattaaaag tataactgca ttgttaaaat atgtatctat atgctcataa gtgaatgtga
      901 atttgcattc atttatgggc tattttttat gcaaatttat gtaaacggga acctcgacaa
      961 agttttacta caatttcaga cgaataccta attaaaacaa caccacagta tgaatgttca
     1021 cttattgttt gaaaatatgt cataaacaaa cagattataa acatattatg ttgatttatt
     1081 gtttgtttat gtttataatt taaacgtaat caaattgaaa gccttttctc aagattttca
     1141 taacctacaa ataaacatgt catattttaa gaaaggtatt tcaaaattaa agtaaaaagg
     1201 agtcttatta tggcgattat tattggttca gatgaagctg gcaaacgatt aaaagaagtc
     1261 atcaaatcat acttattaga caataaatat gatgttgttg acgtaacaga aggacaggaa
     1321 gttgactttg ttgatgcaac tttggctgta gcaaaagatg ttcaaagtca agaaggtaac
     1381 ttaggtattg ttatcgatgc atttggtgca ggtagcttta tggttgctac aaaaatcaaa
     1441 ggcatgattg ctgctgaagt atcagatgaa cgctctggat atatgactag aggccataac
     1501 aattcaagaa tgatcactat ggggtctgaa attgttggag acacattagc gaaaaatgtc
     1561 gttaaaggtt ttgttgaagg taaatacgat ggtggtagac accaaatccg tgtagatatg
     1621 cttaataaaa tgtgctaggg aggaaaaatt actatgaaga ttgcattagg atgcgaccat
     1681 attgttacag atacaaaaat gcgtgtatct gaatttttaa aatcaaaagg acatgaagtc
     1741 attgacgtag gaacatacga tttcacaaga acacattatc caatttttgg taaaaaagtt
     1801 ggcgaacaag ttgttagcgg taatgcagac ttaggtgttt gtatttgtgg aacaggtgtt
     1861 ggtattaaca atgctgtaaa taaagtacct ggcgttcgtt cagcactagt acgtgatatg
     1921 acatcagcgt tatatgctaa agaagaatta aatgcgaacg ttattggctt tggtggacgt
     1981 attataggtg agttattaat gtgcgatatt atcgatgcat ttattaacgc tgaatataaa
     2041 ccaactgaag agaataaaaa attaatcgct aaaattaaac atttagaaac aagcaatgca
     2101 gatcaagctg atccacattt ctttgatgaa ttcttagaaa aatgggacag aggcgaatac
     2161 cacgattaag ggacgacaca tatgatttta actttaacat taaatccatc cgttgatatt
     2221 tcatatccat taacagcatt gaaattagat gatgttaatc gtgttcaaga agtaagtaaa
     2281 acagctggtg gtaaaggttt aaatgtaaca agagtacttg cgcaagtagg cgagccagtc
     2341 cttgcgagtg gttttattgg cggtgaatta ggtcaattta ttgctaaaaa attagatcat
     2401 gccgacatca agcatgcatt ttataacatt aagggtgaaa cacgaaactg cattgcaatt
     2461 ttacatgaag ggcaacaaac agaaatttta gagcagggac cggagattga taatcaagag
     2521 gctgcaggat ttattaaaca ttttgaacaa ttattagaaa aagttgaagc agttgctatt
     2581 tcaggtagct tacctaaagg attaaatcaa gattattatg cacaaattat tgaacgatgc
     2641 caaaacaaag gtgtgcctgt gatattggac tgctcgggtg caactttgca aacagttttg
     2701 gaaaatccat acaaaccaac agtcattaaa ccaaacattt ctgaactata tcaattgctt
     2761 aaccagccac ttgatgaaag tttagaaagt ttgaaacagg cagtgtcaca acccttattc
     2821 gaagggattg agtggattat tgtgtcttta ggtgcacaag gtgcattcgc taagcacaat
     2881 catacattct atagagtgaa tattccgaca ataagcgtat taaatccggt tggttcaggt
     2941 gattctacag ttgctggaat tacttcagca attctcaatc atgaaaacga ccacgattta
     3001 ctaaaaaaag ccaatacttt aggcatgctg aatgcgcaag aagcacaaac aggttatgtc
     3061 aatttaaata attatgatga tttattcaat caaattgaag ttttagaggt gtaaaaaatg
     3121 tcaaaatcga atcaaaaaat cgcatcaatt gaacaactaa gtaataacga aggtatcatt
     3181 tctgcattag catttgacca acgtggtgct ttaaaacgca tgatggcaaa acatcaaact
     3241 gaagagccaa ctgtagctca aattgaacaa ttaaaagtat tagttgcgga agaattaaca
     3301 caatatgcat cttcaatttt attagatcca gaatacggtt taccagcatc tgatgcacgt
     3361 aacaaagatt gtggattatt gctagcttat gaaaaaactg gttatgacgt aaatgctaaa
     3421 ggtcgtttac ctgactgttt agtagagtgg tctgcaaaac gtttaaaaga acaaggtgca
     3481 aatgctgtta aattcttact ttactatgat gttgatgatg ctgaagaaat taacattcaa
     3541 aagaaagcat acattgaaag aatcggttca gaatgtgttg cagaagacat tcctttcttc
     3601 ttagaagtat taacatacga tgacaacatt cctgataacg gtagtgttga attcgcaaaa
     3661 gttaagccta gaaaggttaa cgaagcaatg aaattattct cagaaccacg ctttaatgtt
     3721 gatgtattga aagttgaagt accagtaaac atgaagtatg tagaaggctt tgctgaaggt
     3781 gaagtggttt atacaaaaga ggaagcggca caacacttta aaaatcaaga tgcagcaact
     3841 catttaccat acatttactt aagtgctggt gtttctgcgg aattattcca agaaacttta
     3901 aaatttgctc acgaagctgg tgctaaattc aacggtgtac tttgtggccg tgctacatgg
     3961 tctggtgctg ttcaagtata cattgaacaa ggtgaagatg ctgctcgtga atggttacgt
     4021 acaactggtt tcaaaaatat tgatgactta aataaagtat taaaagatac tgcgacatct
     4081 tggaaacaaa gaaaataagt ataaaagggg attaatgcta tgaatagaga agaagtccaa
     4141 ttattaggtt ttgaaattgt tgcatttgca ggggatgcac gttctaagtt tttagaagca
     4201 ttgacagcag ctcaagctgg agattttgca aaagcagatg cattgattga agaaggaaac
     4261 aattgcattg ctgaagcgca tagagcacaa acaagcctgt tagctaaaga agcacaaggt
     4321 gatgatattg catacagtgt aacgatgatg catggtcaag accatttaat gacaacaatt
     4381 ttactgaaag atttaatgaa gcatttatta gaattttata aaagagggtg atgacaatga
     4441 tgcaaaaatt gatcgcacaa attgaaaaag ggaagccatt tttcgagaaa ttatcacgta
     4501 acatatattt aagagccatc cgtgatggat ttatttctgc aatgcctgtc atcttattct
     4561 caagtatctt tctattaatt gcatacgtgc caaatatatt tggttttaaa tgggataaag
     4621 gcatggaagc cattttaatg aaaccatata actatacgat ggggcttgta gcatttttag
     4681 tagcaggaac gacagcgaaa tctttaacag attcatttaa ccgtaagcta gaaagtacaa
     4741 atcaaattaa ctttatttct acaatgctag cagcgatgtg tggattcttg ttcttagctt
     4801 ctgatccagc caaagatggt ggattcttaa gtgcatttat gggaactaaa ggtttattaa
     4861 cagcattttt atcagcattt gtaactgtta ttgtttataa cttctgtgtg aagcgcaata
     4921 ttacaattaa aatgccgaaa gaagtaccgc cgaatatttc acaagtattt aaggacttaa
     4981 ttccattttc agcggtaatc atcattcttt atgcattaga tttagtcatt cgcaacagct
     5041 ttaaatcaaa tgtagcggaa ggtattttaa aattattcga accattattt acagcagcag
     5101 atggatggat tggtgtcaca attatctttg gtgcctttgc attattctgg tttgtaggaa
     5161 ttcatggtcc atcaatagta gagccagcaa ttgcagccat tacatatgcg aatatcgaag
     5221 cgaacttcaa gttgcttcaa gctggagaac acgcagataa aattattaca tcaggtacac
     5281 aaatgtttat cgttactttt ggaggtacgg gtgcaacatt ggtcgtcccg ttcatgttta
     5341 tgtggatgac gaaatctaaa cgaaacaaag cgattggacg tgcatcagtt gtaccaacat
     5401 tctttggtgt aaacgaacca attttatttg gtgcaccact agtattgaat cctgtgttct
     5461 ttattccatt tgtattagca ccaattgtta acgtatggat tttcaaactt ttcgttgaag
     5521 tgttaggaat gaatagtttt agtgtgaatt taccttggac aacaccaggt ccattgggca
     5581 ttatcatggg tacaggtttt ggtttatggt cattcgttct agctattact ttgattgttg
     5641 tagatattat tatttactac ccattcctaa aagtttatga tagtgaaatt cttgatgaag
     5701 aagaaggacg taaagaaagt aattcagatt taaaagaaaa agttgcagca aactttgata
     5761 cgaaaaaagc tgattcaatt ttagcggcaa gtggtgtatc agacgatgca gctaaagctt
     5821 caaatatcac agaacaaaca aatgtcttag tactttgtgc cggtggtggt acaagtggat
     5881 tattagccaa tgcacttaat aaagcagctg aagaatatca tgtgccagtg aaagcggcag
     5941 ctggcggata tggcgcacat atggacatta tgaaagagta ccaattaatc attttagcgc
     6001 cacaagttgc gtctaactat gaagatatta agcaagatac agatagatta ggtattaaat
     6061 tagcgaagac tcaaggtgcc gaatatatca aattgacacg agacggccaa gcagcccttg
     6121 atttcgttca acaacaattt gaaaattaag aataggagtt tttcatatga ctaaaacatt
     6181 acctgaagat tttatttttg gtggagcaac agcagcatat caagcagaag gtgcgacaaa
     6241 tacggatggt aaaggtcgtg tagcatggga tacgtatttg gaagaaaact attggtatac
     6301 tgcagaacca gcgagtgatt tttataacag atatccagtg gatttagaat taagtgaaaa
     6361 attcggtgtt aatggtatcc gtatttcaat tgcatggtca cgtatcttcc caaatggata
     6421 tggcgaagtt aaccctaaag gtgttgagta ttaccataag ttatttgcag agtgtcataa
     6481 acgtcatgtt gaaccatttg taacattgca tcattttgat acaccagaag tgttacacaa
     6541 agatggcgat tttctaaatc gtaaaacgat tgactacttt gtagattatg ctgaatattg
     6601 ttttaaagaa ttcccagaag taaaatattg gactacattc aatgaaattg gacctattgg
     6661 tgatggccaa tatttagtag gtaaattccc tccaggtatt aaatatgatt ttgaaaaagt
     6721 tttccaatct catcataata tgatggttgc tcatgcaaga gcagtgaaat tattcaagga
     6781 tggcggctat caaggagaaa ttggtgttgt acacgcattg ccaacaaaat atccgttcga
     6841 tccatcaaat cctgaagatg ttcgtgcagc agaattagaa gatatcatcc ataataaatt
     6901 catcttagat gcaacatatt taggtaagta ttctcgtgaa acgatggaag gtgtgcaaca
     6961 tatcttatct gttaatggcg gtaaattaaa tattacagat gaagattatg ccattttaga
     7021 tgcagccaaa gatttaaacg acttcttagg tatcaattac tacatgagtg actggatgag
     7081 aggttacgat ggagaatctg aaatcacgca taatgcgaca ggtgataaag gcggttctaa
     7141 ataccaactt aaaggtgtcg gacaacgtga atttgatgtc gacgtacctc gcacagactg
     7201 ggactggatg atctatcctc aaggtttata tgatcaaatc atgcgtgttg ttaaagatta
     7261 tcctaactat cacaagattt atatcactga aaatggttta ggatataaag atgaatttat
     7321 tgaatctgaa aaaacagttc atgatgatgc acgtattgat tatgtaagac aacatttgaa
     7381 cgttattgca gatgctatta aagatggtgc aaatgttaaa ggttacttta tttggtcatt
     7441 aatggatgtg ttctcttggt caaatggtta tgaaaaacga tatggtttat tctatgtcga
     7501 ttttgagact caagaacgtt atcctaagaa aagtgcttat tggtacaaag agttagccga
     7561 aacaaaagaa attaaataat tatagatatt aaaaggccta tcagtcgagt gctggtaggt
     7621 ctttatttat aaaaagagat aaattaaaca tgcatataca tatttatata ttaatcaatg
     7681 ggtgacacac cattgaaagc taaagaagca atcaattaac aaatgttaac ggacaacttt
     7741 ccaaagtcat cttaaaagtt aatgatgtat tcaaaatcaa ctaaaggaca tttttaatca
     7801 catcctttat acttatataa aagacatgaa tgggagttaa aagtgtgaag aaaatttttg
     7861 taattattac gacattgtta gcggtcgcta ttattatagg ctctattatt acggtggtgt
     7921 ttagtcaacg acaggcacaa acatttaaaa tacaacaaca gcaatttgtt aaaaagccaa
     7981 ttccaacatt gttcttacat ggatttggtg gtagtgccaa ttctgaaaag tttatggtga
     8041 agcaagcaga aaaaagaggg gtaactaaag atatcatcac tgcttacgtt tcaaaggatg
     8101 gtgcagtgac atttaaaggg aaactaagaa aagatgcagt gaatcctatt gtgaaaattg
     8161 aacttgaaaa taacagacaa ggctatttgg ataagaatgc tgcgtggttt aaaaatgtac
     8221 tgactaagtt acaatctgaa tacaatttcg ataaattcaa ctttgtcggt cactcaatgg
     8281 ggaacttaac atttgcacaa tatatgatga catatggtaa cgacaagtca ctgccacagt
     8341 taaacaaaca agtgaatatt gcagggacat ttaatggtgt tttaaatatg aatgaagatg
     8401 tcaatgaaat tacagttgat aaagatggta aaccaagtcg aatgaatcaa ccttatcagc
     8461 aattacgcgt gctaaaagat atttataaag gtaaaggtat cgaagtgcta aatatttatg
     8521 gagatttgaa agatggtaca cattcagacg gcagagtgtc taatagttct tcgaaatcat
     8581 taaaatattt actgggaaat agtccgaaaa gttatcgaga atctaaatat gaaggtgagc
     8641 ctgcacaaca tagtcagctt catgagaatg aaaatgttgc taatgagtta atcgactttt
     8701 tatggaaaaa ataaagcaac tgctagtttt tcgggggcaa aaaagcttgt aatattagag
     8761 cttttcaaaa attatctatt gtttgagagt ttccataaaa tgtctaagtc gtaacatttt
     8821 aaatttatga ttatagcaaa tataatgtgg ttgaagttac atgaatgaat cagtgcttaa
     8881 gtcaaaatat aacaactaag aatgtatttg catacagaca tcacgaagac acaattcaaa
     8941 cttaacgctt aagtaacaaa taagtaaaag tttcaaataa agaaaggtga ttaatgatgc
     9001 aaaataaaca aattcttttt aacaaaatac cagaaggcat gcctcaagag gatacattca
     9061 aaattgaaga gactgacacg cctaaacttg agagcgatgg cgtacttgta caaacgctgt
     9121 atatttcagt ggatccatat atgagaggac gtatgactaa ggcagattcg tatgtacagc
     9181 cttttgaaat tggtaaaccg attgttagcc atgtcgttgc aaaagtaatt gattcgacac
     9241 tcgcagatta taaaaaagga gatgtcgttg taggtatgtt gccttggcga atcattaatc
     9301 atgtccaagc ggatcaaatt actaaagttc caacgacaga tgtaccactt gatttatatt
     9361 taagtgtttt aggaatgcca ggacaaacag catatcatgg tctgcttgat attggacaac
     9421 ctaaagccgg tgatactgtt gtggtttcag ctgcatctgg tgctgtaggt tcagttgtag
     9481 gtcaaattgc taagattaaa ggttgccgtg tagtaggtat tgcgggtggc gacaaaaaag
     9541 taaattattt aacagggaca ctgggctttg acgctggtat tgattataag aaagaggatt
     9601 ttgcagaagc gttagcacaa gcagtaccag atggtattga tgtttatttt gaaaatgttg
     9661 gtggaacagt tggcgatgaa gtatttaagc atttaaatcg ttttgcacgt gtaccagtat
     9721 gtggcgcaat ttcgtcatat aatcatcctg aagcagatat cggaccacgt attcaaggta
     9781 cgcttattaa gaagcaagta atgatgagag gcttcttggt aagtgaattc gctaatgctt
     9841 ttaaagaagc tagtgaacag cttgcaacat gggttcaaga aggtaaaatt cagtcacaag
     9901 taaccattga agatggtttt gaaaatgcgc cacatgcatt taaaaattta tttactggcg
     9961 ataactttgg taaacaagtt attaaagtaa cggaataata aagtatacgt tttaacaaac
    10021 taactttgtt aattgactaa aatttaataa ttttttatct aaagcaaatt tgttcagtaa
    10081 catggtagaa ataagtctaa aatgtaatac gaaagtcgtc tgtaattatt tatatattca
    10141 aatgaaaaag gagtcatgat gctgcgatat aagcacacat ggcttctatt ttgtataata
    10201 agaaataagt tcataaatga ggtgaaacga tatgaaaatg ataggatttg aaaagccctt
    10261 taaattggaa gaaggaaatt tatttaaagt atacgaacaa agaaagccaa cgccagaaaa
    10321 tgatgatatc ttagtcaaag taaatagcat tagtgtaaat cctgtagata caaagcaacg
    10381 acaaatggaa gtcactcaag cacctcgtgt acttgggttt gatgctattg gtactgttga
    10441 agctatcgga ccagacgtta cattatttag tccgggtgac gttgtatttt atgctggatc
    10501 gccaaataga caaggatcaa atgcgacata tcaattggtg tcagaagcta tagttgcaaa
    10561 agcaccacac aacataagtg ctaatgaagc ggttagtcta ccgctaactg gtattacggc
    10621 atatgaaacg ttttttgata catttaaaat ttcacataat cctgctgaaa acgaagggaa
    10681 gtctgtctta atcattaatg gtgccggtgg tgtaggcagt atagccactc aaattgctaa
    10741 acggtatggt ttaactgtca ttacaacagc atcacgacaa gaaacaacag agtggtgtga
    10801 aaagatgggc gcagatatcg tattgaatca taaagaggat ttagtgcgtc aatttaaaga
    10861 aaaagaaatt ccattagtag attatatatt ttgcacctat aatacagact tgtattacaa
    10921 tactatgatt gaactgataa aaccattagg gcatattaca actatcgtag cttttaatga
    10981 agatcaagat ttaaatgcac taaaattaaa aagtataaca tttacacatg aatttatgtt
    11041 tgcgcgtcca attcatcgaa cgcctgatat gattaaacaa catgagtatt tagaagatat
    11101 tacgaaaaat atagaatcag ggcattacca accaacaacg acacaagttt ttgaaggttt
    11161 atcacctgaa aacttatatc aagcacatca attgttggaa aaacaatcaa tgattggaaa
    11221 attagtaatt aatatatagt ttagtaagta aagtcgcctt atgcagaaaa tactgtatag
    11281 ggcgacttat tttttgtgtc tagcgactat tgattctaaa tggaaatatg gcaactttgc
    11341 aggtatcaat tcaataatgc attagaaagg cattacttaa atacaataaa atactttgtt
    11401 gctcaatgat aaataatttg agtgtgtttt atatatcatt tttaaaagat gaggcattat
    11461 taattgtgtc tatataaaat tataaatgat taaaatgttg cagttatggg tatgaactta
    11521 ccattacaca gaaagggttt gtatttatga aaaataaatc agtaagtcta gtgttttggg
    11581 tatctttagt gatatgtacg atatttgttg cttttggagc aatatttccg aaacagttag
    11641 aaaaactcac gcaaaacatt actacattta ttgcgctaca tttttcttgg tattatttat
    11701 tgctcgtttt agttatttta tttgtctgtg tgtatatttt attttcgaga tacgcaaaca
    11761 ttacattagg tgaagaaggc gaagatcctg aattctcatt gccgtcttgg tttgctatgt
    11821 tatttagtgc tggtatggga ataggacttg tgttctggac aacggcagaa ccaatcagtc
    11881 atgccttcaa attaacgcct attcataaag ctggcacgca atctgcaatt aatgatgcaa
    11941 tgcagttttc atttttccat tgggggattc atgcatgggc agtctatggt attgtcgcgc
    12001 ttgtgtttgc atattttagc tttcataaag gatatcctgg cttagtcagc gctacattga
    12061 cgcctttatt aggtgaaaag gcaatgcgtg gacctttagg tggtgccatt gatgtattgg
    12121 cagtcatagc tacagtaaca ggcgttgctg caacattagg tttcggtgca ttgcaaataa
    12181 acgaaggttt acatttttta tttaatgtac cgagtaattt cacgatgcaa gtaatcttga
    12241 ttgtcattgc aaccatatta tttacttggt cagcatggtc aggtatagat aaaggtatca
    12301 aaacgttgag taatatcaac atgttacttg cttttgtagt acttattggt ctttttattg
    12361 ttgggccaac gctatacatt ttaaatacat ttacgaatgg tctaggtaat tatattgcaa
    12421 atttctttag tatgagttta cgcattccgt caggcggtca aaaattccaa tggttacaaa
    12481 attggacaat cttttactgg gcatggtgga tatcttgggc accatttgta ggtatattta
    12541 ttgcacgtgt gtcaaaagga cgtacgatta aagaatttat tctaggtgta ctttttgtcc
    12601 cggcattagt ttgctttata ttctttgcgg tattcggcgc gtcagcaatt tacttacaag
    12661 ataatcatat tgccgatatt gctaaagcag cgacagaaac agcaactttt gcaacgctac
    12721 aacattaccc actaggtttt gtattaagct taattacttt acttgttatt atgattttct
    12781 ttgttacatc agcggattcg gcaacttatg ttttaggtat gttgagttca agaggtgata
    12841 ttaatccgaa gtcatttgtc aaagtgagtt ggggtatcat tatggcatta tttgcaatta
    12901 ttatgattta cacaggtggt acgcaagcga tacaaaattt attaatcatt gctgcgttac
    12961 cattttccgt ggtcattata gcgatgatat ggtcgctttt aaaatctttg agtgaggaga
    13021 aaccgagaaa tagtaataag gttttaatta aacatcgtga cccagatatt ttagaatacc
    13081 gttcttcaaa acatattgat gaaaattaat taataatgag gttttattta caaaaactag
    13141 ggaatatatt actaaatggt acttcatctt atatattaaa aataaattta aagttattaa
    13201 tttattatgt ttaagaaaaa aactgatggg tacatattta atatgttttt aaattaactt
    13261 taactaagga ggtgtcgtta ggggtgaaac gacttaaaaa ctttatcctt ggcttattaa
    13321 tagtagtaat cgtggggttc ctgttattca tgtatatcca agatggacgt ataactgaat
    13381 atcaagacta cttccttcaa tttgaatggt tccaaccatt acttatttca cttgctgcac
    13441 tattgatatt aataggtctt atattagtat ttagtatctt caaacctacg catcgaaaac
    13501 ctggattata caaagacttc gatgatggtc atgtttacgt atctcgtaaa gctgttgaaa
    13561 agacggcatt cgatacggtt gcaaaatatg atcaagtaag acaaccaaat gtagttgcga
    13621 aactatataa caaaaaaaat aaatcttata tcgacattaa aactgacttt ttcgtaccaa
    13681 ataatgttca agttcaatct ttaactgaag caattcgttc ggatattaaa aagaatgtag
    13741 aatatttcac agaaatgcca gtacgtaagt tagaagttaa tgtgagagat cagaaaactt
    13801 ctggtccacg agtgttgtaa gggaggtata tcatggctaa caatcataac caaaacggac
    13861 aagactctac acaacaagtg attaattttt taaaagtatt taaatggaga atcgttggtt
    13921 tcctagcgtt tctattaatc gcgatattat tcttaacgtt aggtttttgg aaaacagtac
    13981 ttatcatcgt tttatgttta attggtgtag gtattgggta tatgaaagac cgtaagcaag
    14041 attttatgaa ttttttaaat cgatggagtt aattcatcaa tttagaattt aattaaaaaa
    14101 atataaaaat taacaagcaa aaggagaatc tattatgact gtagataaca ataaagcaaa
    14161 acaagcatac gacaatcaaa ctggtgttaa cgaaaaagaa agagaagagc gtcaaaaaca
    14221 acaagaacaa aatcaagagc ctcaattcaa aaataaatta acattctctg atgaagttgt
    14281 tgaaaaaatt gctggtatcg ctgcacgtga agttaaaggt atcttagaca tgaaaggtgg
    14341 cttaactgat acattcacta atgcattctc aagtggaaat aacgttactc aaggtgtatc
    14401 tgttgaagtt ggtgaaaaac aagctgctgt agacttaaaa gtaattttag aatatggtga
    14461 atcagcacct aaaatcttcc gtaaagtaac tgaattagta aaagaacaag ttaaatatat
    14521 tactggttta gatgttgttg aagttaacat gcaagttgac gatgtaatga ctcaaaaaga
    14581 atggaaacaa aaacacgaaa aaaataatga aaacaataac caagaaagac aaggtttaca
    14641 ataagttatt cgataaaaag ctcggcactc aatttaagtg ccgagctttt ttgtgatttt
    14701 taataatttg aatatggaaa atgta
//
LOCUS       DAMZKC010000056        14656 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.056, whole genome shotgun sequence.
ACCESSION   DAMZKC010000056 DAMZKC010000000
VERSION     DAMZKC010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14656
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.056"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            331..1716
                     /gene="fumC"
                     /locus_tag="POB55_001269"
     CDS             331..1716
                     /gene="fumC"
                     /locus_tag="POB55_001269"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HDI6500239.1"
                     /translation="MSVRIEHDTFGEIEVPADKYWGAQTERSKRNFPVGKERMPIEVV
                     YGFAQLKRAAALANFDLGKLSEAKKDAIVYACDQILSGELDEHFPLVVWQTGSGTQSN
                     MNVNEVVSYVANMYLKDHQIDESIHPNDDVNKSQSSNDTFPTAMHVALYQEVETKLEP
                     ALKLLRNTLKEKEDKFDSIIKIGRTHLQDATPIKLGQEISGWRYMLDRCEIMLSESKK
                     HILNLAIGGTAVGTGINAHPEFGDKVAHYISENTGYPFVSSENKFHALTAHDEVVQLH
                     GTLKALAGDLMKIANDVRWLASGPRAGLAEISIPENEPGSSIMPGKVNPTQCEMLTMV
                     AVQVMGNDTVVGFASSQGNFELNVYKPVIMHNTLQSIYLLADGMETFNNNCAVGIEPI
                     EENIDNYLNQSLMLVTALNPHIGYEKAAQIAKKAHKEGLTLKESAIQTGYVTEEQFEA
                     WIKPEDMVDPH"
     gene            complement(2177..2998)
                     /locus_tag="POB55_001270"
     CDS             complement(2177..2998)
                     /locus_tag="POB55_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDI6500240.1"
                     /translation="MKFKIPENFNDLSLRDIFQQLKVPKKDLHHLNMSKDITINDKPA
                     RLMDKVHTGDDVFVPTIDEKSNYVPSYRYAQIKYEDDDMAIVMKPKGVKTHPNDLKES
                     NTLMNHVIYTIDSDYVEPIHRLDQETVGLLIVAKNPLMKKILDRMLEDNDITRIYKAN
                     VKALLPLKPQTIDMPIGKDKFHSNKRRVSPTGQRAITHILTSKMIKEAVCQLEIKLDT
                     GRTHQIRVHLAEIGHPVIGDPLYGDSTLRQLELESYKIEFVHPLTKEVISVSLDD"
     gene            3161..4273
                     /locus_tag="POB55_001271"
     CDS             3161..4273
                     /locus_tag="POB55_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HDI6500241.1"
                     /translation="MEQRTRLALLKEIAEFLNEETEMYSMTQGALKYLIEGSNFTTGW
                     IFFINSVGEHELVSHVALPQSLTADHCHYIKDGSCWCVKAFNQRRLMKASNIVNCSRI
                     NLASKAFPSQNDNITHHATVPLKSGQEQFGILNVASPNTEIYSDEDLELLESVAFQLG
                     SAIKRIYLTDREKEAAKINERNRLARDLHDSVNQMLFSVKLTAHAAYGMSNESIAKQA
                     FKTIEETSQNAVNEMRALIWQLKPVGLEQGLIHALTAYSKLIHIQLNVNVEGLIDLSN
                     EIEENIYRALQECINNVKKHADTNKMDLTLKQMNDILYIDVIDYGQGFEIDNVQIASS
                     HGINNIKQRVKLLRGRVTFHSQPTKGTQIQFTIPIK"
     gene            4295..4918
                     /locus_tag="POB55_001272"
     CDS             4295..4918
                     /locus_tag="POB55_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDI6500242.1"
                     /translation="MNKVILVDDHYIVRQGLRFLLSTIENIEVLQDFADGETFLEYLK
                     EHEHPDIVLLDLVMPGMNGIEITEYIKAHYPDIKVLVLTSYVDDEHVISAINKGADGY
                     EMKDVEPQQLIETIRRVMNGEKMIHPKAQDVFETVSQKPHYTNKLSKREIEVLREMVK
                     GKTNKEIAETLFVSEKTIKTHVSHIFSKLQVSDRTQAAIYAMENKLI"
     gene            5274..5738
                     /locus_tag="POB55_001273"
     CDS             5274..5738
                     /locus_tag="POB55_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI6500243.1"
                     /translation="MDRQSFTDLIQTKFKMVRIEAGYTQDTMAQTIGLSKKTLVQIEK
                     ERVLPNWTTCISICALFRDSEVLNSTFGCDPLEIVQTISRNHCAYPNHAPTSDIYWNN
                     IETRNGYILQSNKVSNIYRVLNPDNQPIFGTSKMREAETYFNRNAKEELVHI"
     gene            5917..7041
                     /locus_tag="POB55_001274"
     CDS             5917..7041
                     /locus_tag="POB55_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HDI6500244.1"
                     /translation="MNALFIIIFMIVVGAIIGGITNVIAIRMLFHPFKPYYIFKFRVP
                     FTPGLIPKRREEIATKIGQVIEEHLLTETLINEKLKSEQSQQAIESMIQQQLQKLTKD
                     QLSIKQITSQIDIDLEQVLQTNGNQYIESQLNNYYTKHQNQTIASLLPNQLVTFLDQH
                     VDNATDLLCDRARNYLSSAKGTQDINDMLDTFFNEKGKLFGMLQMFMTKESIADRIQQ
                     ELIRLTSHPKARTIVTSLITNEYQTFKDKPLNELLDASQFNEIAENLSVYVTTYASKQ
                     ANKPVVTLMPQFVDYLEGQLSSKLANLIIEKLSIHLSTIMKKVDLRGLIEEQINTFDL
                     DYIEKLIIEIANKELKLIMSLGFILGGIIGFFQGLVAIFV"
     gene            7110..7454
                     /locus_tag="POB55_001275"
     CDS             7110..7454
                     /locus_tag="POB55_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002498160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HDI6500245.1"
                     /translation="MAVNLYDYANQLEQALRESEEYKAIKEAFANVKANEESKKLFDE
                     FRETQINFQQKQMQGEEIAEEDLQKAQEQAQAIEKDENISALMNAEQKMSQVFQEINQ
                     IIVKPLDEIYAD"
     gene            7947..8045
                     /locus_tag="POB55_001276"
     CDS             7947..8045
                     /locus_tag="POB55_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500246.1"
                     /translation="MSVGSLAVLTITEIAFPTEIFEKNQRTLQIKF"
     gene            8336..9532
                     /locus_tag="POB55_001277"
     CDS             8336..9532
                     /locus_tag="POB55_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HDI6500247.1"
                     /translation="MVKFIHCSDLHLDSPFKSKSHISPKIFEDVQKSAYESFKNIVDI
                     ALQQDVDFVIIAGDLFDSENRTLRAEIFLKQQFERLQNEQIFVYVCHGNHDPLSSKIS
                     SNWPDNVSVFSNKVETYEAITKSGETIYIHGFSYENRASYENKIDEYPSSQGQKGIHI
                     GVLHGTYSKSSVNERYTEFILEDLNSKLYHYWALGHIHERQQLSDMPVINYSGNIQGR
                     HFNEQGEKGCLLIEGDHLKLKTKFYPTQYIRFEEATIETDKTSKQGLYEVIQNFKEQV
                     REEGKAFYRLTLVINSETLISPQDLLQVEEMITDYEENENQFVYIDELKVQYAQNDES
                     PLVNEFSAELLVDQTVFDKAMSDLYLNPRASKFLDDYGTFDHTALVNRAEEILKAEMR
                     GEQNDN"
     gene            9522..12458
                     /locus_tag="POB55_001278"
     CDS             9522..12458
                     /locus_tag="POB55_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDI6500248.1"
                     /translation="MIIKSLEIYGYGQFVQRKIEFNKNFTEIFGENEAGKSTIQAFIH
                     SILFGFPTKKSKEPRLEPRLGNQYGGKLVLILDDGLEIEVERIKGSAQGDVKVYLPNG
                     AVRDDAWLQKKLNYISKKTYQGIFSFDVLGLQDIHRNLNEKQLQDYLLQAGALGSTEF
                     TSMREVINRKKDELYKKSGKNPIINQQIEQLKQLESQIREEEAKLETYHRLVDDRDKS
                     SRRLENLKHNLNQLSKMHEEKQKEVALHDHSQEWKSLEQQLNIEPITFPEKGVDRYEK
                     ARAHKQSLERDIGLRNERLAQLKEEATQLEPVKQSDIDAFISLNQQENEIKNKEFELT
                     AIEKDIANKQRDKDELQANIGWSETHHDVDSSEAMKSYVSEQIKNKQEQAAYIKQLER
                     SLEENKIEDNAVHSELDSVEEKIVPEETFEKKKEYSQQVIELNEKENLYSKLKERFEI
                     EQQEKQKRQKLLRTTFILLTLVGIGLTAFSFISNNMLFGIIFAVLTLVFVIGIIMSKS
                     KEVDYSEAITDEIEEIKAQLAILDENYDLDFDLDEQYRIRDHWQQALKNKDILEEKRQ
                     YIEGRLNDAKGRHDELQSTVENVKDELYLSSKISNDLIVDSISTMANIKALDQHISDL
                     NQQRQQLVQELDTFYNHAEAVTKSQFVYFNKLSLFHDVQQWLKSAEDTNEKWRINAEN
                     TKLVTNELNHLNAQLEENNKEITALFDFINVGTEEDFYQHHEDYQTYTSNLSRFNDLT
                     KYLENQNYSYELSSSLSEKTTAQLEEEDHLLATQVDEYNEQYLEMQAQVSDLSAQINH
                     METDTTLANLRHEYHSLKNQLNDIAKDWASLSYLQSLVDEHIKQIKDKRLPQVINEAV
                     EILKHLTDGRYTMINYNEDSITVKHVNGQLYDPVELSQSTKELLYVALRISLIKVLRP
                     YYPFPLIVDDAFVHFDKKRTEKMLNYLRSLSEHYQVLYFTCVKDNIVPSKEVITLNKI
                     EEGGKR"
     gene            12455..13396
                     /gene="yhaM"
                     /locus_tag="POB55_001279"
     CDS             12455..13396
                     /gene="yhaM"
                     /locus_tag="POB55_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM"
                     /protein_id="HDI6500249.1"
                     /translation="MRNIENLNPGDSVDHFFLVHKATQGVTAQGKDYMTLHLQDKSGE
                     IEAKFWTATKNDMATIKPEEIVHVKGDIINYRGNKQMKVNQIRLATTEDQLKTEQFVD
                     GAPLSPAEIQEEISHYLLDIENANLQRITRHLLKKYQERFYTYPAASSHHHNFASGLS
                     YHVLTMLRVAKSICDIYPLLNKSLLYSGIILHDIGKVRELSGPVATSYTVEGNLLGHI
                     SIASDEVVEAARELNIEGEEIMLLRHMILSHHGKLEYGSPKLPYLKEAEILCYIDNID
                     ARMNMFEKAYKKTDKGQFTDKIFGLENRRFYNPESLD"
     gene            complement(13517..14479)
                     /locus_tag="POB55_001280"
     CDS             complement(13517..14479)
                     /locus_tag="POB55_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="foldase"
                     /protein_id="HDI6500250.1"
                     /translation="MKMINKLIVPVTASALLLGACGASATDSKENTLISSKAGDVTVA
                     DTMKKIGKDQIANASFTEMLNKILADKYKNKVNDKKIDEQIEKMQKQYGGKDKFEKAL
                     QQQGLTADKYKENLRTAAYHKELLSDKIKISDSEIKEDSKKASHILIKVKSKKSDKEG
                     LDDKEAKQKAEEIQKEVSKDPSKFGEIAKKESMDTGSAKKDGELGYVLKGQTDKDFEK
                     ALFKLKDGEVSEVVKSSFGYHIIKADKPTDFNSEKQSLKEKLVDQKVQKNPKLLTDAY
                     KDLLKEYDVDFKDRDIKSVVEDKILNPEKLKQGGAQGGQSGMSQ"
BASE COUNT         5395 a         2104 c         2524 g         4633 t
ORIGIN      
        1 ttttatgcga gtttaacagg agttttaggt acgcttgttg ctctaaatcc atatacgaca
       61 attgttagtg ctgtattata tatacttaca gctattgcta cggttgaagc tactaaaaca
      121 aagtatcgta attaaactta gttcaacaat aactaaataa tgatagataa cacactaatc
      181 gtatgaccgt atcactgtaa taaagggtga gtacgtgcga ttagtgtatt tttatttcaa
      241 attatgaaaa cgttttaatc ataaattgct caattaaatt atttaatgat atactacagt
      301 taaataaaca gtgataaggg gagaaattga atgtcagtaa gaattgaaca tgatactttt
      361 ggagaaatag aagtacctgc agataaatat tggggtgctc aaacagaaag aagtaaacgc
      421 aatttcccag ttggtaaaga gcgtatgcca atcgaagtag tttatggttt tgcacaacta
      481 aagcgtgcag cagcattagc taattttgat ttaggaaaat taagcgaggc aaagaaagat
      541 gccattgtat acgcatgtga tcaaatttta tcaggtgaat tagatgaaca cttcccacta
      601 gttgtatggc aaacaggaag cggtacacaa agtaatatga atgtgaacga agtagtaagt
      661 tatgttgcta atatgtattt aaaagatcat caaattgatg aaagtatcca cccaaatgat
      721 gatgtaaata aatcacaaag ttcgaatgat acattcccaa ctgctatgca cgttgcatta
      781 tatcaagagg ttgaaacaaa attagaacct gcattaaaac ttttaagaaa tactttgaaa
      841 gaaaaagaag acaaatttga ttcaattatt aaaattggtc gtacacattt acaagatgca
      901 acgccgatca aactaggaca agagattagt ggctggcgtt atatgcttga ccgttgcgaa
      961 ataatgttat ctgaatctaa gaagcacatt ttaaatcttg ccatcggtgg tacggctgtt
     1021 ggtactggta ttaatgcgca tcctgaattt ggtgataaag tggcacatta tatttcagaa
     1081 aatacgggtt atccatttgt atcttctgaa aataaattcc atgcattaac agcacatgat
     1141 gaagtagtac aactacatgg aacattgaag gcgctagcag gagacttaat gaaaattgct
     1201 aatgatgtga gatggttggc ttcagggcca cgagctggtt tggcagaaat ttctatccct
     1261 gaaaatgaac caggttcatc aattatgcct ggtaaagtta atcctacaca atgtgaaatg
     1321 ttaacaatgg ttgcagtcca agtaatgggt aatgatacag ttgttggctt cgcaagttca
     1381 caaggtaact ttgaattgaa tgtttataaa ccagttatta tgcataatac actacaatca
     1441 atttaccttt tagctgatgg tatggaaaca tttaataaca attgcgcagt gggcattgaa
     1501 ccaatcgaag agaatattga taattattta aatcaatcat taatgttagt tactgcatta
     1561 aatccacata ttggttatga aaaagcagct caaattgcta agaaagccca taaagaaggt
     1621 ttaactttaa aagaatctgc aattcaaact ggatatgtta cagaagaaca atttgaagca
     1681 tggattaaac cagaagatat ggtagatcct cattaattaa atattaatat aaaataaagt
     1741 ttgggcatta caggtagatg tagtgttcaa gctttatttt ttgtctatgt caaattgaac
     1801 ttataagtct gatttgatat agagccgtga aagaaaaaac ttttcatgga gtccgtttta
     1861 ttatagaacc tattttttat atgatgaatt gttgtacatg tattggccaa acaacctgtg
     1921 ataaaaatct tatgatgtca tgtattgtcc atagatacac gtaacgcagt tgaaaaagac
     1981 tacaagttat tatccaagga tgttataaaa tgaataatta ctaaaatatc aaaatataaa
     2041 atcctaagca tcgtaaaaaa caaaaaaccg aacatctgaa aaaattaata attttccatc
     2101 tatccgattt aagttgatac ttgatttagt tacaatgcgg tattgcatgt ttaaatatca
     2161 tgcaaaacta atcaaattag tcatccaaag aaacggaaat gacttcctta gtcaagggat
     2221 gcacaaactc tattttgtaa ctttcaagtt ctaattgtct taacgttgaa tcaccatata
     2281 aaggatcacc aataacaggg tgaccaattt cagctaaatg cacacgaatt tgatgagtac
     2341 gtccagtatc caacttgatt tcaagttggc acacagcttc ttttatcatt tttgaagtta
     2401 aaatgtgtgt aattgcacgc tgtccagtag gagacacacg tcgtttattc gaatggaatt
     2461 tatctttacc aattggcata tcaatcgttt gtggtttcaa aggtaaaagt gccttaacat
     2521 ttgctttgta tatccgcgta atatcattgt cttctaacat gcgatcaaga atttttttca
     2581 ttaaaggatt tttagcaaca attaataatc ctactgtttc ctggtccagt cgatgaattg
     2641 gttcgacata gtcactatca atagtgtaaa tcacatgatt cattaaagta ttgctttctt
     2701 ttaaatcatt agggtgagtc ttaacacctt taggtttcat tacgattgcc atatcatcgt
     2761 cttcgtattt aatttgtgca taacgataac ttggaacata attacttttt tcatcgatgg
     2821 ttggaacaaa tacatcgtcg ccagtatgca ctttatccat taatcgcgca ggtttatcat
     2881 taatagtaat atctttagac atatttaaat gatgtaaatc ttttttaggt accttaagtt
     2941 gttggaaaat atctcgtaaa cttaagtcat taaagttttc tggtatttta aatttcataa
     3001 aatcctccta tagtctttaa tatcatatca caaatcagtt ctccgaaata taactattga
     3061 gtaatcaagg ccattttatc atgttcacgt ctaagattta aaaatataat aactcaatgt
     3121 ataatagtta atacacgtaa aaatgaattg gagcgatttg atggaacaaa ggacgcgact
     3181 agctttatta aaagagattg ccgaattttt aaacgaagaa actgaaatgt atagcatgac
     3241 ccaaggtgcg ttaaaatatt taattgaggg cagtaatttc acgacaggct ggatattttt
     3301 catcaatagc gtaggtgaac atgaacttgt atcacatgtg gctctaccac aatctttgac
     3361 agcagaccac tgtcactata ttaaagatgg gtcttgttgg tgtgtgaaag catttaatca
     3421 acgtaggcta atgaaagcgt cgaatatcgt caattgttct cgtattaact tagcctctaa
     3481 agcatttcct agccaaaatg acaatattac acatcatgca acggtaccac ttaaatcggg
     3541 tcaagaacaa ttcggcattt taaatgtagc gtcaccaaat actgaaatat atagtgatga
     3601 agatttagag ttgctagagt ctgtcgcatt tcaattaggg tcagccatca aacgtattta
     3661 tttaacagac cgtgaaaaag aagcagcaaa aattaatgaa cgtaatcgtt tagctagaga
     3721 tttacatgat tcagtaaatc aaatgttatt ttctgtaaaa ctaacagcac atgctgcgta
     3781 tggcatgtca aacgaatcca tcgctaaaca agcattcaag acgattgagg aaacgagtca
     3841 aaatgccgtt aatgaaatgc gtgcattgat ttggcaactt aagccagttg gacttgagca
     3901 aggtttaatt catgctttga cagcatatag taaattaatc catatacaat taaatgtaaa
     3961 tgtagaaggt ttaatcgatt tatctaatga aatcgaagaa aacatatata gagcattaca
     4021 agagtgtatt aataatgtta agaaacatgc tgatacgaat aaaatggatc ttacattaaa
     4081 acaaatgaac gatatactat atattgacgt gattgattat ggtcaaggat ttgagatcga
     4141 taatgttcag atagcttcat cacacggtat taataacata aaacaaagag ttaaattatt
     4201 aagaggtaga gtgacctttc attctcagcc aacaaagggt acacaaatac aattcacaat
     4261 tcctataaaa tagcttgatt taaggagata acccatgaac aaagtaatat tagtagatga
     4321 ccattatatt gtgcgacaag gattgcgatt tttattatcc acgattgaaa acatagaagt
     4381 tttacaagac tttgcagatg gagaaacatt tttagaatat ttaaaagagc atgagcaccc
     4441 tgatattgtg ctattagatt tagtgatgcc tggcatgaat ggtattgaaa ttacggaata
     4501 tattaaggca cattatccgg atattaaagt tttggtatta acaagttatg ttgatgatga
     4561 acatgtaatt tcagcaatca ataaaggtgc tgatggttat gaaatgaaag acgttgagcc
     4621 tcagcaatta attgaaacta ttagacgagt tatgaacggt gaaaaaatga tacatcctaa
     4681 ggcacaagat gtattcgaaa cagttagcca aaaaccacac tacacgaata agttgtcaaa
     4741 gagagaaatt gaagtgttac gtgaaatggt taaaggtaaa acaaataaag agattgcaga
     4801 aactttattt gtatctgaaa aaacaattaa aacacatgtc agtcatatat ttagtaaatt
     4861 acaagttagc gatcgtacac aagcagcaat ttatgcaatg gaaaataagt tgatttagaa
     4921 tgagctttta aatatttgtc gaaaaataga caatttttaa tctgattttg atgaattttt
     4981 tatagcgata gggtttatta tacagttttc tttgtaaatt tgatatcatt ataaaagtgt
     5041 aatttttatc aaatctagtg attatgcata aaattttact tcatttactt tcgcttcatt
     5101 ataaagtaaa cttaactctt gttgacttga tcaaaattta atacaaatgt gaaattgact
     5161 cttttcattt tattaaaaat atattaagat gtaatctgtg tttcaagaaa tgtgtattat
     5221 tgcaatttct tgtgtccgga taaaattccc caaacagaaa ggtagataac agaatggata
     5281 gacagagttt tacagattta attcaaacaa aatttaaaat ggttcgtata gaggctggtt
     5341 atacgcaaga tactatggcg caaacaattg gactttctaa aaagacttta gtacaaatcg
     5401 aaaaagagag agtattacca aactggacaa cttgtatttc aatttgtgca ttattcagag
     5461 actctgaagt attaaacagt acatttggct gtgatccatt agaaatcgtt caaacaattt
     5521 caagaaatca ctgtgcatat ccaaaccatg caccaacaag tgatatttat tggaataaca
     5581 ttgagactcg taatgggtac attttacaaa gtaacaaagt aagcaatatt tatcgtgtat
     5641 taaatccaga taaccaacca attttcggta cttctaaaat gagagaagct gaaacatact
     5701 ttaacagaaa tgctaaagaa gaattagtac atatctaggc ctatatactt ttaataaata
     5761 ataaaagcga tgtaacacac ctgtttcgag aggtgaggca tcgctttttg tatgtaaact
     5821 aataataaat tgagcagaaa gtgaaatgat taatgaatta aaaaataaaa atagtggtat
     5881 aatgaataac tatgttgtca caaaggagtc aaaattatga atgcactatt tattatcata
     5941 tttatgattg tagtaggggc gataattggg ggcattacta atgtaattgc aattagaatg
     6001 ctgtttcacc cctttaaacc atactatata tttaaattta gagtcccatt tacaccaggt
     6061 ttaataccga aaagacgcga agaaattgca actaaaattg gccaagtgat tgaagagcat
     6121 ttgcttacag aaactttaat taatgaaaaa ttaaaaagcg agcaatcaca gcaagcaata
     6181 gaatctatga ttcaacagca gttacaaaag ttgacgaaag atcaattgtc aataaaacaa
     6241 attacttctc aaatcgatat tgatttagaa caagtattac aaactaatgg aaatcaatat
     6301 attgaatcac aattgaataa ttattataca aagcatcaaa accaaacaat agcatcttta
     6361 ttgccaaatc aacttgtaac atttttagat cagcatgtag ataacgcaac agacttatta
     6421 tgtgatcgtg caaggaatta tttatcatct gcaaaaggca cacaagatat taatgatatg
     6481 ttggatactt ttttcaatga gaaaggtaag ttatttggta tgttgcaaat gtttatgaca
     6541 aaagagagca ttgcagatcg cattcaacaa gaacttatac gtttaacatc tcatcctaaa
     6601 gcaagaacaa ttgtgacatc gttaattacc aatgaatatc aaacttttaa agataaacca
     6661 ttgaatgaac tgttagatgc atcgcagttt aatgaaatcg ctgaaaatct atcagtgtat
     6721 gtaacaacat atgcaagcaa acaagcgaat aagccagtgg ttacgttaat gcctcaattc
     6781 gttgattatt tagaaggtca actctctagc aaattagcaa atttaatcat tgaaaaatta
     6841 tcgattcact tatctacaat tatgaaaaaa gtggatttac gaggattaat tgaagaacaa
     6901 attaatacgt ttgacttaga ttatattgaa aaattaatta tagaaattgc taacaaagaa
     6961 ctaaagttga ttatgtcatt aggtttcatt ttaggtggta ttattggatt tttccaaggt
     7021 ttggttgcaa tctttgtcta aaaaagcgag atgtggtaaa gtaatatcga acgttttaat
     7081 aaacatcaca acaaaggagt gctataacca tggcagtaaa tttatatgat tatgcaaatc
     7141 aattagaaca agctttaaga gaaagcgaag aatacaaagc aatcaaagaa gcattcgcta
     7201 atgtaaaagc taacgaagaa tctaaaaagt tattcgacga gttccgtgaa actcaaatta
     7261 acttccaaca aaaacaaatg caaggtgaag aaattgctga agaagattta caaaaagcgc
     7321 aagaacaagc gcaagcaatt gaaaaagatg aaaacatctc tgcattaatg aatgctgaac
     7381 aaaaaatgag tcaagtattc caagaaatca accaaattat cgttaaacca ttagacgaaa
     7441 tttacgctga ctaatattaa aataatattt ttcaatcctt taaatagagg aatagactgc
     7501 cgacagagtc cgagactttg tgggtagttt ttttagtttt gataacggaa gttagaggct
     7561 ctctgtcaaa ttgggcagat gcgttcagta tcggaattta ggctactaaa catgtcctag
     7621 ctttagctca gaatggacga cggaagctgg gcaatcaaac atggctagct ctatgccaaa
     7681 atgggcagat gagcttgata acggaagtta ggctagtaaa cgtgtcctaa cttccttttt
     7741 gcattttgaa gtgtaaagtt ttcaacataa tactattagt tcggtcatgt atcggactga
     7801 tggaaaagcg tttcactttt aatggctcat taagaacggc ctgaaaatgt ttggcgtatt
     7861 tagtgcaatg atagttttga catttagttt ctaattggtc attactgccg agcaaatcta
     7921 gtagagtaat catgtaaatc tttaatgtgt cagttggttc actagcggtg ttaacaatta
     7981 cggaaattgc atttccgact gaaatttttg aaaaaaatca acgcacgcta caaataaaat
     8041 tttaaactgt tataaatgtg tctcaatttc atatgttcat cgacgatatg aagcgtatta
     8101 tggtaaaatg aagaaataat aaacttgtta ataaataaaa catcacgatt tgactaaagc
     8161 actttattat tgtgtagata ataatttttt aacgaaataa aaatggcgac tggttttaat
     8221 aaatcagcta atgaatcact acacctataa gtatgaatat agtgattaga atgctttgta
     8281 tagttggatt ttgcaaaatt gatgttagaa cctttaatta aggagatatg gatgtatggt
     8341 taaatttatt cattgttcgg atttacattt agatagtcct ttcaaatcta agagtcatat
     8401 aagccctaaa atttttgagg atgttcaaaa aagtgcttat gaaagtttta aaaacattgt
     8461 agatattgca ttacaacaag atgttgattt tgtaattatc gcgggtgatt tatttgatag
     8521 tgaaaacaga acattacgtg ctgaaatttt cttaaaacag caatttgaac gtttacaaaa
     8581 tgaacaaatc tttgtttatg tttgtcatgg taatcacgac ccattatcat caaagatttc
     8641 atcaaactgg ccagataatg tttctgtatt ttcaaataaa gttgagacgt atgaagcaat
     8701 tactaaatct ggtgaaacaa tttatattca cggatttagt tatgaaaata gagcaagtta
     8761 tgagaacaag attgatgaat atccatcaag tcaaggccaa aaaggcatac atattggtgt
     8821 cttgcatggt acgtatagta aatcttcagt taacgaaaga tataccgagt tcattttaga
     8881 agatttaaac agtaaattgt atcattattg ggctttaggt catatacatg aacgtcaaca
     8941 attaagtgat atgcctgtaa ttaactattc aggtaatatt caaggtagac attttaatga
     9001 gcaaggtgaa aaaggttgct tattaatcga gggtgaccac ttaaaattaa agactaaatt
     9061 ttatcctaca cagtatatta gatttgaaga agcaactatt gaaacggata agacatctaa
     9121 gcaaggttta tacgaggtca ttcaaaactt taaagaacaa gtgagagaag aaggaaaagc
     9181 cttttatcgt ttaacgcttg ttattaatag tgagacatta atttcacctc aagatttatt
     9241 acaagttgaa gaaatgatta cagattatga agaaaacgaa aatcaatttg tatatattga
     9301 tgagttaaaa gtacaatatg cacaaaatga tgagtcacct ttagttaatg aattttcagc
     9361 ggaattatta gtcgatcaaa ctgtttttga taaagcgatg tcagatttat atttaaatcc
     9421 aagggcatct aagttcctag acgattatgg aacattcgac catacagcat tagttaatcg
     9481 tgctgaagaa atattaaaag ctgaaatgag aggtgaacaa aatgataatt aaatcacttg
     9541 aaatttatgg ttacggtcaa tttgttcaac gtaaaattga atttaataaa aacttcactg
     9601 aaatttttgg tgaaaatgaa gcgggtaaat cgacgattca agcattcatc cattcgatat
     9661 tatttggttt tccaactaaa aagtctaaag aaccaaggct agaaccacgt ctaggtaacc
     9721 aatacggtgg taaattagta cttattcttg atgatggctt agagattgaa gttgaacgaa
     9781 ttaaaggcag tgctcaaggt gatgtgaaag tatatttacc taatggtgct gtgcgtgatg
     9841 atgcttggtt acaaaagaaa cttaattata tttctaaaaa gacatatcaa ggtatctttt
     9901 catttgatgt actagggctt caagacattc atagaaatct aaatgaaaaa caattgcaag
     9961 attatttatt acaagcaggg gctttaggat caactgaatt cacgtcaatg cgcgaagtga
    10021 ttaatcgtaa aaaagatgaa ttatataaaa aatcaggtaa aaatccgatc attaatcaac
    10081 aaattgagca attaaaacaa ctagaaagtc aaattcgtga agaagaagct aagctagaaa
    10141 catatcatcg cttagtagat gatcgagata aatcatcacg tcgattagag aatttaaagc
    10201 ataatttaaa tcaattatca aaaatgcatg aagaaaaaca aaaagaggtt gctttacatg
    10261 atcattcaca agaatggaag tctctagaac aacagttaaa tattgagcca atcacattcc
    10321 cagaaaaagg tgtggatcgt tacgaaaaag cacgagcgca taagcaatcg ttagaaagag
    10381 atattggttt aagaaatgag cgtttagctc aacttaaaga agaagcgact caattagagc
    10441 cagttaaaca atctgatatt gacgccttca ttagtttgaa tcaacaagaa aatgaaatta
    10501 aaaataaaga atttgaactt actgcaatcg aaaaggatat tgcgaataaa caacgtgata
    10561 aagatgaatt gcaagcaaat attggttggt ctgaaacgca tcatgacgta gatagttcag
    10621 aggcaatgaa aagttatgtc agtgagcaaa tcaagaataa acaagaacaa gctgcataca
    10681 ttaaacaatt agaacgtagt ttagaagaaa ataaaatcga agataatgcg gttcatagcg
    10741 aactagattc tgttgaagaa aaaatagttc ctgaagagac ttttgaaaag aaaaaagaat
    10801 actcacaaca agtcattgaa ttaaacgaaa aagagaactt gtatagcaaa ttgaaagaac
    10861 gtttcgaaat tgagcaacaa gaaaaacaaa aacgtcaaaa attgttacga acaacgttta
    10921 ttcttttaac attagtaggt attggtttaa ctgccttctc atttatttca aacaacatgt
    10981 tgtttggtat tattttcgca gtattaactt tagtatttgt tatcggtatc atcatgtcta
    11041 aatcaaaaga agtagattat agcgaggcca tcacagatga aatcgaagaa attaaagcac
    11101 aactagccat tttagatgaa aattatgact tagattttga tcttgatgag caatatcgca
    11161 ttcgtgatca ctggcaacaa gctttgaaaa ataaagatat tttagaagag aaacgtcagt
    11221 atattgaagg tcgtttaaat gatgcgaaag ggcgtcatga tgaattacaa tcgacagttg
    11281 agaacgttaa ggatgaatta tacttatcat ctaaaatttc aaatgattta attgttgata
    11341 gtatttctac aatggcgaat attaaagcat tagatcaaca tatcagtgat ttaaatcaac
    11401 aacgccagca attggttcaa gaattagata cattctacaa tcatgctgaa gctgttacaa
    11461 aatcacaatt tgtttatttc aacaaattat ctttattcca cgatgtgcaa caatggttga
    11521 aatcagctga agacacgaat gaaaaatggc gtattaatgc tgaaaatacc aaacttgtta
    11581 caaatgaatt aaatcattta aatgctcaat tagaagaaaa taataaagaa attacagcac
    11641 tattcgattt tatcaatgtt ggtactgaag aagattttta ccaacatcat gaagattacc
    11701 aaacttatac tagtaatttg agtcgtttta atgatttaac taaatatctt gagaaccaaa
    11761 actattctta tgaattaagt tctagtttaa gtgaaaaaac aactgcacaa cttgaagaag
    11821 aagatcattt attggctact caagttgacg aatataatga gcaatatctt gaaatgcaag
    11881 cacaagtcag tgatttaagt gcacaaatca atcacatgga aactgataca acgcttgcta
    11941 atttaagaca tgaatatcac agtcttaaaa atcaacttaa tgatatcgca aaagattggg
    12001 caagtttaag ttatttacaa agtttagttg atgaacacat taaacaaatt aaagataaac
    12061 gtttgcctca agttattaat gaagcggtag aaatattgaa gcatttaaca gatggcagat
    12121 atacgatgat taactataat gaagattcaa ttacggttaa acatgttaat ggtcaattat
    12181 atgatcctgt tgaactaagt caatctacaa aagaattact ttatgtagct ttacgtatca
    12241 gtttaattaa ggtactaaga ccatattatc cgttcccatt aattgttgat gatgcatttg
    12301 ttcattttga taaaaaacgt actgaaaaaa tgttgaatta tttacgatca ttatcagaac
    12361 actatcaagt actatacttt acatgtgtaa aagataatat tgttccatca aaagaagtga
    12421 ttacattaaa caaaatagag gaaggcggga aacgatgaga aatatagaga atctaaatcc
    12481 cggagattca gttgatcact ttttcttagt gcataaagct acacagggtg taacagcaca
    12541 aggtaaagat tatatgacat tacatttgca agataaaagt ggtgaaattg aagcgaaatt
    12601 ttggacggct acaaaaaatg atatggcaac aatcaagcct gaagaaattg tacatgttaa
    12661 aggtgacatc ataaactatc gcggaaataa acagatgaaa gtcaaccaaa ttagactagc
    12721 gacaactgaa gatcaattaa aaacagaaca atttgtagat ggtgcacctt tatcaccggc
    12781 agaaatacaa gaagagattt ctcattattt gctagatatt gaaaatgcta atttacaacg
    12841 tatcacacgt catttattga aaaaatatca agaacgattt tacacatatc cagctgctag
    12901 ttctcatcat cataactttg cgagtggctt aagctatcat gtattgacga tgttacgtgt
    12961 tgcaaaatca atttgtgaca tttatccatt gttaaacaaa agtttgttat atagtggtat
    13021 tattttgcat gatattggta aagttagaga attgagtggt cctgttgcga cgtcgtatac
    13081 agtcgaaggt aacttattag gacacatctc gattgcgagt gatgaagtag ttgaagcagc
    13141 tcgtgaattg aacattgaag gagaagaaat catgttgtta agacatatga ttttatctca
    13201 tcatggtaag ttagagtatg gttcgccaaa actgccatac ttaaaagaag cagaaatttt
    13261 atgctatatc gataacatcg atgctagaat gaatatgttt gaaaaggctt ataaaaaaac
    13321 tgacaagggt cagtttacag ataaaatatt tggtcttgaa aatcgtagat tctacaatcc
    13381 tgaatcactc gattaagaat taaaagatat cggacagatg agtatactga aacatgagct
    13441 catctgtccg atttttaatg cgaaaaaacc gagcggccgt ggtatgattt ttgaaccacg
    13501 gtcgctcggt tttgtgttat tggctcatgc cggattgtcc gccttgtgcg ccaccttgtt
    13561 taagtttttc agggtttaag attttatctt cgacaactga tttaatatca cgatctttaa
    13621 agtcaacatc gtattctttt aatagatctt tgtatgcatc agttaataat tttggatttt
    13681 tttgtacttt ctgatcgact aatttttctt tcaggctttg tttttcactg ttaaagtctg
    13741 ttggtttatc agctttaata atatgatatc caaagcttga tttaacaacc tctgatactt
    13801 caccatcttt aagcttaaat agtgcttttt caaaatcttt atcagtttgt cctttaagaa
    13861 cataacctaa ttcgccatct tttttagctg aaccagtatc cattgattct tttttagcga
    13921 tttcaccaaa tttacttgga tcttttgaaa cttctttttg aatttcttca gctttttgtt
    13981 tcgcttcttt atcgtctaag ccttctttgt cgcttttctt agatttaact ttaattaaaa
    14041 tgtgtgaagc tttcttgctg tcttctttaa tttcagaatc agagatttta attttatctg
    14101 atagtaattc tttatgataa gcagcagtac gtaaattttc tttatattta tcggctgtta
    14161 aaccttgctg ttgaagggcc ttttcaaatt tatctttacc gccgtattgc ttttgcattt
    14221 tttcaatttg ttcgtcaatc ttcttatcat taactttatt tttatattta tcagctaaaa
    14281 ttttatttaa catttcagta aatgatgcat ttgcaatttg atctttaccg atttttttca
    14341 ttgtatctgc aactgttacg tctccagctt tagaagaaat taatgtattt tctttagagt
    14401 ctgtggcact agcgccacaa gcgcctaata ataaagcact agctgttacc ggaacgatta
    14461 atttgtttat catcttcata gttgaaactc ctttgtaagt taatcatcgt gaatataaca
    14521 tagttttata ttatcaccaa tcaataaccc atgacatcag actagagcat taggtttagg
    14581 ggtagaaatt gatgaaaata aaaaaacaag gtctatatac atttgattgt aagctattac
    14641 atagcctaaa aacaaa
//
LOCUS       DAMZKC010000057        14619 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.057, whole genome shotgun sequence.
ACCESSION   DAMZKC010000057 DAMZKC010000000
VERSION     DAMZKC010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14619
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.057"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            40..1269
                     /locus_tag="POB55_001281"
     CDS             40..1269
                     /locus_tag="POB55_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HDI6500251.1"
                     /translation="MSLRDEALEMHKRNQGKLEVKPNVKVTNKEELSLAYSPGVAEPC
                     KDIYEDKRKVYDYTIKGNTVAVITDGTAVLGLGNIGPEASIPVMEGKAVLFKSFAGIN
                     GVPIALNTTDTEEIIKTVKLLEPNYGGINLEDISAPRCFEIEERLKKETNIPVFHDDQ
                     HGTAIVTMAGLVNALRVVNKDIAKIKVVLNGAGAAGIAIVKLLYAYGVRNMVMCDSRG
                     AIFEGRSYGMNPTKDVVAKWTNKDKIEGSLEEVVKDADVFIGVSVANALSQDMVKSMA
                     DNPIIFAMANPNPEIIPDDAKAAGARVVGTGRSDYPNQINNVLAFPGIFRGALEVEAT
                     HINEEMKKAAVEAIADLIDSSELNEDYCIPGPFDKRVAPSVARNVAKAAMESGVARIE
                     VDPQDVYDKTMKLTDLQ"
     gene            1464..2321
                     /locus_tag="POB55_001282"
     CDS             1464..2321
                     /locus_tag="POB55_001282"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HDI6500252.1"
                     /translation="MFKDFFNRTKKKKYLTVQDSKNNDVPAGIMTKCPKCKKIMYTKE
                     LAENLNVCFNCDHHIALTAYKRIEAISDEGSFTEFDKGMTSANPLDFPSYLEKIEKDQ
                     QKTGLKEAVVTGTAQLDGMKFGVAVMDSRFRMGSMGSVIGEKICRIIDYCTENRLPFI
                     LFSASGGARMQEGIISLMQMGKTSVSLKRHSDAGLLYISYLTHPTTGGVSASFASVGD
                     INLSEPKALIGFAGRRVIEQTINEKLPDDFQTAEFLLEHGQLDKVVHRNNMRQTLSEI
                     LKIHQEVTK"
     gene            2321..3265
                     /locus_tag="POB55_001283"
     CDS             2321..3265
                     /locus_tag="POB55_001283"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HDI6500253.1"
                     /translation="MLDFEKPLFEIRNKIESLKESQDKNDVDLQEEIDMLEASLERET
                     KKIYTNLKPWDRVQIARLQERPTTLDYIPYIFDSFMELHGDRNFRDDPAMIGGIGFLN
                     GRAVTVIGQQRGKDTKDNIYRNFGMAHPEGYRKALRLMKQAEKFNRPIFTFIDTKGAY
                     PGKAAEERGQSESIATNLIEMASLKVPVIAIVIGEGGSGGALGIGIANKVLMLENSTY
                     SVISPEGAAALLWKDSNLAKIAAETMKITAHDIKQLGIIDDVISEPLGGAHKDIEQQA
                     LAIKSAFVAQLDSLESLSRDEIANDRFEKFRNIGSYIE"
     gene            3534..4502
                     /gene="pfkA"
                     /locus_tag="POB55_001284"
     CDS             3534..4502
                     /gene="pfkA"
                     /locus_tag="POB55_001284"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002462395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDI6500254.1"
                     /translation="MKKIAVLTSGGDSPGMNAAVRAVVRTAIYNEIEVYGVYHGYQGL
                     LNDDIHKLELGSVGDTIQRGGTFLYSARCPEFKEQEVRKVAIENLRKRGIEGLVVIGG
                     DGSYRGAQRISEECKEIQTIGIPGTIDNDINGTDFTIGFDTALNTIIGLVDKIRDTAS
                     SHARTFIIEAMGRDCGDLALWAGLSVGAETIVVPEVKTDIKEIADKIEQGIKRGKKHS
                     IVLVAEGCMTAQDCQKELSQYINVDNRVSVLGHVQRGGSPTGADRVLASRLGGYAVDL
                     LMQGETAKGVGIKNNKIVATSFDEIFDGKDHKFDYSLYELANKLSI"
     gene            4524..6281
                     /gene="pyk"
                     /locus_tag="POB55_001285"
     CDS             4524..6281
                     /gene="pyk"
                     /locus_tag="POB55_001285"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDI6500255.1"
                     /translation="MRKTKIVCTIGPASESEEMIEKLINAGMNVARLNFSHGSHEEHK
                     GRIDTIRKVAKRLDKIVAILLDTKGPEIRTHNMKDGIIELERGNEVIVSMNEVEGTPE
                     KFSVTYENLINDVQVGSYILLDDGLIELQVKDIDHAKKEVKCDILNSGELKNKKGVNL
                     PGVRVSLPGITEKDAEDIRFGIKENVDFIAASFVRRPSDVLEIREILEEQKANISVFP
                     KIENQEGIDNIAEILEVSDGLMVARGDMGVEIPPEKVPMVQKDLIRQCNKLGKPVITA
                     TQMLDSMQRNPRATRAEASDVANAIYDGTDAVMLSGETAAGLYPEEAVKTMRNIAVSA
                     EAAQDYKKLLSDRTKLVETSLVNAIGISVAHTALNLNVKAIVAATESGSTARTISKYR
                     PHSDIIAVTPSEETARQCSIVWGVQPVVKKGRKSTDALLNNAVATAVETGRVSNGDLI
                     IITAGVPTGETGTTNMMKIHLVGDEIANGQGIGRGSVVGTTLVAETVKDLEGKDLSDK
                     VIVTNSIDETFVPYVEKALGLITEENGITSPSAIVGLEKGIPTVVGVEKAVKNISNNM
                     LVTIDAAQGKIFEGYANVL"
     gene            complement(6629..7990)
                     /locus_tag="POB55_001286"
     CDS             complement(6629..7990)
                     /locus_tag="POB55_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDI6500256.1"
                     /translation="MAEKLQRELSNRHIQLIAIGGAIGTGLFLGAGQTIALTGPSILL
                     TYIIIGFMLFMFMRGLGEIIIQNTEFKSFADVTNTYIGPFAGFVTGWTYWFCWIITGM
                     AEVTAVAKYVSFWFPEIPNWISALFCVLLLMSFNLLSARLFGELEFWFSIIKIATIIG
                     LIVVGFVMILFAFKTQFGHASFTNLYEHGIFAKGASGFFMSFQMALFSFVGIEMIGVT
                     AGETKDPVKTIPKAINSVPIRILIFYVGALAVIMSIIPWQQVDPDNSPFVKLFALIGI
                     PFAAGLINFVVLTAAASSCNSGIFSNSRMLFGLSSQQQAPPNFSKTNKYGVPHVAIFA
                     SSALLLVAALLNYIFPDATKVFTYVTTISTVLFLVVWGLIIIAYINYSRKNPDLHKNA
                     TYKLLGGKYMGYLIFVFFIFVFGLLFINVDTRRAIYFIPIWFILLAFMYLRYKRIAAK
                     SNK"
     gene            8337..9458
                     /locus_tag="POB55_001287"
     CDS             8337..9458
                     /locus_tag="POB55_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001662816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HDI6500257.1"
                     /translation="MAELQRGLEGVIAAETKISSIIESQLTYAGYDIDDLAENAQFEE
                     VIFLLWNYRLPNEEELAHLKGKLNQYMTLNPRVYTHFEEYVTDHVHPMTALRTSLSYI
                     AHFDPDAENESDENRYERAMRIQAKVASLVTAFARVRQDKEPLKPNPDLSYAANFLYM
                     LRGELPTDIEVEAFNKALILHADHELNASAFTARCAVSSLSDMYSGIVAAVGSLKGPL
                     HGGANEQVMTMLSEIGSIENVDAYLDEKFANKDKVMGFGHRVYKDGDPRAKYLREMSR
                     QITKDAGREELFEMSVKMEKRMAEEKGLIPNVDFYSASVYHCMEIPHDLFTPIFAVSR
                     SAGWIAHILEQYKDNRIMRPRAKYIGETNRKYIPLEERK"
     gene            9507..10775
                     /gene="icd"
                     /locus_tag="POB55_001288"
     CDS             9507..10775
                     /gene="icd"
                     /locus_tag="POB55_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HDI6500258.1"
                     /translation="MTAEKITQGTEGLNVPNEPIIPFIIGDGIGPDIWKAASRVIDAA
                     VEKAYNGEKRIEWKEVLAGQKAFDTTGEWLPQETLDTIKEYLIAVKGPLTTPIGGGIR
                     SLNVALRQELDLFTCLRPVRWFKGVPSPVKRPQDVDMVIFRENTEDIYAGIEFKEGTT
                     EVKKVIDFLQNEMGATNIRFPETSGIGIKPVSKEGTERLVRAAIQYAIDNNRKSVTLV
                     HKGNIMKFTEGSFKQWGYDLALSEFGDQVFTWQQYDEIVEKEGRDAANAAQEKAEKEG
                     KIIIKDSIADIFLQQILTRPAEHDVVATMNLNGDYISDALAAQVGGIGIAPGANINYE
                     TGHAIFEATHGTAPKYAGLNKVNPSSVILSSVLMLEHLGWQEAADKITDSIEDTIASK
                     VVTYDFARLMDGAEEVSTSAFADELIKNLK"
     gene            11165..11869
                     /locus_tag="POB55_001289"
     CDS             11165..11869
                     /locus_tag="POB55_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDI6500259.1"
                     /translation="MSQKVLVVDDEHSIVTLLKYNLETAGYEVVVAFDGDEALEKVES
                     EQPDLIILDVMLPKKDGIDVCKTVRTNKNLVPILMLTAKNDEFDRVLGLELGADDYMT
                     KPFSPREVVARVKAILRRSQFVKEVEEVDDDITIGSIRIRPDYFEVYKHNELLELTPK
                     EFELLLYLIERQGRVITREHMLNTVWNYEFAGDSRIVDVHISHLRDKLEDNPKKPQLI
                     KTVRGLGYKLERPKEQ"
     gene            11869..13530
                     /locus_tag="POB55_001290"
     CDS             11869..13530
                     /locus_tag="POB55_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HDI6500260.1"
                     /translation="MKFHHRLMLLISSILIISFVTLGIIIHASITYVLSEKHEQALAT
                     DARSYIYLVQDNEIDKVKEIVKQQNIDLLITKHDKKVFSSGKLKAIPNSDSYIQKRDF
                     FIFNKKVNGYHVWIKGYNNDITEMHWTLWKYLILTCLVVLICLYFASRSFKRTLIRPI
                     QEVTYATQLLANGYYHIRVPESNVVETKALFVSTNDLARRLQKLNNEQKIQSNRLKTT
                     IENIPSAILMIDRNGKIVVANKAYYEQFNISHNIEQVGYHGYVNTEIEQLILESFKVE
                     KPIYEQLEVAINQVHAKYFDISCVPILTRSQKSLQGILVVMHDITNLKQLENLRREFV
                     ANVSHELKTPITSIKGFAETLIDGAKNDAESLDMFLNIILKESNRIESLVTDLLDLSH
                     IEQHTELDTDYMNLSDLTRRIIDNMMTQANQKNISIHTDIEKDVIVKAQESKIAQVIT
                     NLLTNAINYSYEDGDINVRVYRDDFRVIFEVQDFGIGIKLEDQQRIFERFYRVDKARS
                     RDSGGTGLGLSITKHIVEAHQGNIEVNSQVGKGSTFKVILKDYKE"
     gene            14029..>14619
                     /locus_tag="POB55_001291"
     CDS             14029..>14619
                     /locus_tag="POB55_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500261.1"
                     /translation="MKTIQLHKTTLILIAILLFYTFMGILLPLMHDDLQWFSNYNTDI
                     LKVGFASLNGRYIGNIFEIIAVHVSWLRWLSYGLISMGIIWMIMHITRCKAWTSYYLL
                     AFSLMLILPSAIYADTYGWFAGFYNYATSTLISLFIIYYCINAIIYKEKQPVSVTVLF
                     YVLCFFGQLFMENVTLFHCIILILAFLYEFTANRTLN"
BASE COUNT         5220 a         2006 c         2824 g         4569 t
ORIGIN      
        1 tattttttaa aaataaatat tgaattaggg gctgatttca tgtcattaag agatgaagca
       61 ttggaaatgc acaaacgtaa tcaaggtaaa ttagaagtta aaccaaatgt aaaagttact
      121 aataaagagg aattaagttt agcatactca cctggcgttg ctgaaccgtg taaagatatt
      181 tatgaagata aaagaaaagt atatgattac acaattaaag gaaatacagt tgcagttatt
      241 actgatggaa cagcggtatt aggtttaggt aacattggac ctgaagcaag tattcctgta
      301 atggaaggta aagcagtatt attcaaaagc ttcgctggta tcaatggggt gcctattgcg
      361 ttaaatacaa ctgataccga agaaatcatt aaaacagtta agttgttaga acctaattat
      421 ggtggtatta atttagagga tatttcggca ccacgttgtt ttgaaattga agaacgattg
      481 aaaaaagaaa ctaatattcc ggtattccat gacgatcaac atggtacagc aattgtaaca
      541 atggcaggtt tggtaaatgc attgagagtt gttaacaaag atattgctaa aataaaagtt
      601 gtactaaatg gtgctggtgc agcaggaata gccattgtta aattactata cgcgtatggt
      661 gtaagaaata tggttatgtg tgactcaaga ggcgcaattt ttgaaggacg ttcatatggt
      721 atgaatccta cgaaagatgt tgtagctaaa tggacaaata aagataagat tgaagggtct
      781 ttagaagaag tcgtaaaaga cgcagatgta tttatcggag tttctgtagc taatgcgctg
      841 tcacaagata tggttaagag tatggcagat aatccaatta tatttgcaat ggctaatcca
      901 aatcctgaaa taatacctga tgatgccaaa gcggcaggtg cacgagttgt tggtacagga
      961 cgttcagact atcctaacca aattaataat gtattagctt tccctggtat ttttagaggt
     1021 gcattagagg ttgaagctac acatataaat gaagaaatga aaaaggcagc tgtagaagcg
     1081 attgctgatt taatcgatag ttctgaatta aatgaagact actgtatccc aggaccgttt
     1141 gataaacgtg tagcgccatc agttgctcgt aatgttgcta aagcggcaat ggaatctgga
     1201 gtagctagga ttgaagttga tccgcaagat gtgtatgata aaacaatgaa acttacagat
     1261 ttacaataaa aataattaac gtttaattaa attgcttaaa agttagcaat aaagagtcat
     1321 taaaatgatg aaaatgaaag tttatgataa acattcaaca gtcaaacgaa tataaatcaa
     1381 ataaatttaa acccgttttt aactggtcaa gttcagttta aggctctaaa tggttagaac
     1441 agaggttatt tggaggtttt cctatgttta aagatttttt taatcgaaca aagaaaaaga
     1501 aatatcttac agtacaagac tctaaaaata atgatgtgcc tgcaggtatt atgactaagt
     1561 gtccaaagtg taagaaaatt atgtacacaa aagaattagc tgaaaattta aatgtgtgct
     1621 ttaattgtga tcatcatatt gctttaactg cgtataaacg tatagaagca atttctgatg
     1681 aaggatcatt tacagaattc gataagggaa tgacctctgc gaatccatta gattttccaa
     1741 gttatttaga aaaaattgaa aaggaccaac aaaagacagg tcttaaagaa gcagttgtga
     1801 ctggtacagc gcaactagat ggtatgaaat ttggcgttgc tgtcatggat tcacgtttta
     1861 gaatgggaag tatgggatcg gttatcggtg aaaagatatg tcgcatcatt gattactgca
     1921 ctgagaaccg tttaccattt attcttttct ctgcaagtgg tggtgcacgt atgcaagaag
     1981 gtattatttc cttgatgcaa atgggtaaaa ccagtgtatc tttaaaacgt cattctgacg
     2041 ctggactatt atatatatca tatttaacac atccaactac tggtggtgta tctgcaagtt
     2101 ttgcatcagt tggtgatata aatttaagtg agccaaaagc gttgataggt tttgcaggtc
     2161 gtcgagttat tgaacagaca ataaacgaaa aattgccaga tgatttccaa actgcagaat
     2221 ttttattaga gcatggacaa ttggataaag ttgtacatcg taataatatg cgtcaaacat
     2281 tgtctgaaat tctaaaaatc catcaagagg tgactaaata atgttagatt ttgaaaaacc
     2341 actttttgaa attcgaaata aaattgaatc tttaaaagaa tctcaagata aaaatgatgt
     2401 ggatttacaa gaagaaattg acatgcttga agcgtcattg gaacgagaaa ctaaaaaaat
     2461 atatacaaat ctaaaaccat gggatcgtgt gcaaattgcg cgtttgcaag aaagacctac
     2521 gaccctagat tatattccat atatctttga ttcgtttatg gaactacatg gtgatcgtaa
     2581 ttttagagat gatccagcaa tgattggtgg tattggcttt ttaaatggtc gtgctgttac
     2641 agttattgga caacaacgtg gaaaagatac aaaagataat atttatcgaa attttggtat
     2701 ggcgcatcca gaaggttatc gaaaagcatt acgtttaatg aaacaagctg aaaaattcaa
     2761 tcgtcctatc tttacattta tagatacaaa aggtgcatat cctggtaaag ctgcggaaga
     2821 acgtggacaa agtgaatcta tcgcaacaaa tttgattgag atggcttcac taaaagtacc
     2881 agttattgcg attgtcattg gtgaaggtgg cagtggaggt gctctaggta ttggtattgc
     2941 caataaagta ttgatgttag agaatagtac ttactctgtt atatctcctg aaggtgcagc
     3001 ggcattatta tggaaagaca gtaatttggc taaaattgca gctgaaacaa tgaaaattac
     3061 tgcccatgat attaagcaat taggtattat agatgatgtc atttctgaac cacttggcgg
     3121 tgcacataaa gatattgaac agcaagcttt agctattaaa tcagcgtttg ttgcacagtt
     3181 agattcactt gagtcattat cacgtgatga aattgctaat gatcgctttg aaaaattcag
     3241 aaatatcggt tcttatatag aataatcaac ttgagcattt ttatgttaaa tcgatactgg
     3301 gttttaccat aaattgaagt acattaaaac aataatttaa tatttagata ctgaatttta
     3361 actaagatta gtagtcaaaa ttgtggctac taatcttttt ttaattaagt taaaataaaa
     3421 ttcaatattt aaaacgttta catcaattca atacattagt tttgatggaa tgacatatca
     3481 atttgtggta atttagagtt aaagataaat cagttataga aaggtatgtc gtcatgaaga
     3541 aaattgcagt tttaactagt ggtggagatt cacctggaat gaatgctgcc gtaagagcag
     3601 ttgttcgtac agcaatttac aatgaaattg aagtttatgg tgtgtatcat ggttaccaag
     3661 gattgttaaa tgatgatatt cataaacttg aattaggatc agttggggat acgattcagc
     3721 gtggaggtac attcttgtat tcagcaagat gtccagagtt taaggagcaa gaagtacgta
     3781 aagttgcaat cgaaaactta cgtaaaagag ggattgaggg ccttgtagtt attggtggtg
     3841 acggtagtta tcgcggtgca caacgcatca gtgaggaatg caaagaaatt caaactatcg
     3901 gtattcctgg tacgattgac aatgatatca atggtactga ttttacaatt ggatttgaca
     3961 cagcattaaa tacgattatt ggcttagtcg acaaaattag agacactgcg tcaagtcacg
     4021 cacgaacatt tatcattgaa gcaatgggcc gtgattgtgg agatctagca ttatgggctg
     4081 gattatcagt tggtgctgag acaattgtag ttccagaagt gaaaacagat attaaagaaa
     4141 tagctgataa aattgaacaa ggtattaaac gtggtaagaa acactcaatc gttcttgtag
     4201 cagaaggttg tatgactgcg caagattgtc aaaaagaatt atcacaatac atcaatgttg
     4261 ataatagagt gtctgtgtta ggtcacgttc aacgtggtgg tagcccaaca ggtgcggata
     4321 gagttttagc atcacgttta ggtggatatg cggtagactt attaatgcaa ggtgaaacag
     4381 ctaagggtgt tggaattaag aacaataaaa ttgtagcaac atcttttgat gaaatttttg
     4441 atggtaaaga tcataaattt gattatagtc tatatgaact tgctaacaag ttatctatat
     4501 aagatttcag gaggaattat aaaatgagaa aaactaaaat tgtatgtaca attggaccag
     4561 cttcagaatc agaagaaatg attgagaaat taatcaatgc tggtatgaac gttgcacgat
     4621 taaacttttc acatggtagt catgaagagc ataaaggtag aattgataca attcgtaaag
     4681 tagctaaaag attagacaaa attgtagcaa ttttattaga tacaaaaggt ccagaaattc
     4741 gtacgcataa tatgaaagac ggtatcattg aacttgaacg tggtaacgaa gttattgtta
     4801 gcatgaatga agttgaagga acacctgaaa agttctcagt aacatatgaa aacttaatta
     4861 acgatgttca agtaggttca tacattttac ttgatgatgg cttaattgaa ttacaagtta
     4921 aagatattga ccatgctaaa aaagaagtta aatgtgatat tttaaactct ggtgagctta
     4981 aaaacaaaaa aggtgttaac ttacctggcg taagagtaag tttacctggt attacagaaa
     5041 aagatgctga agatatccgt ttcggtatta aagaaaatgt tgacttcatt gcagcaagtt
     5101 tcgtacgtcg tcctagtgat gttttagaaa ttcgtgaaat tttagaagaa caaaaagcta
     5161 acatttcagt attccctaaa attgaaaacc aagaaggtat tgataatatt gcggaaattc
     5221 ttgaagtgtc tgatggttta atggttgcac gtggtgacat gggtgttgaa attccacctg
     5281 aaaaagtacc aatggttcaa aaagatttaa tcagacaatg taacaaatta ggtaaaccag
     5341 ttattacagc tacacaaatg ttagattcta tgcaacgtaa cccacgtgct acacgtgcag
     5401 aagctagtga cgttgccaac gcaatctatg atggtacaga tgcagtaatg ttatctggtg
     5461 aaactgctgc tggtttatat cctgaagaag ctgttaaaac aatgagaaat attgctgtat
     5521 cagctgaagc agcccaagat tacaaaaagt tattgtcaga tcgtactaaa ttagttgaaa
     5581 cttcattagt gaatgctatc ggtatttcgg ttgcacatac agctttaaac ttaaatgtta
     5641 aagcaattgt agctgctact gaaagtggtt caacggcacg tactatctct aaatatcgtc
     5701 cgcattcaga cattattgca gtgactccaa gtgaagaaac tgcacgtcaa tgttcaattg
     5761 tttggggagt tcaacctgta gttaaaaaag gacgtaagag tacagatgca ttgttaaaca
     5821 atgcagttgc aacagctgtt gaaactggta gagtatctaa tggtgattta atcattatta
     5881 ctgctggtgt accaactggt gaaactggaa ctactaatat gatgaaaatc cacctagttg
     5941 gtgacgaaat tgctaatggt caaggtattg gacgtggatc agttgttggt actacgttag
     6001 ttgctgaaac tgttaaagat ttagaaggta aagatttatc tgacaaagtt atcgttacta
     6061 actcaatcga tgaaacgttt gtaccttatg tagaaaaagc tttaggctta attacagaag
     6121 aaaatggtat tacatcacca agtgcaattg ttggtttaga aaaaggtatt ccaacagttg
     6181 taggtgtaga aaaagctgtt aaaaacataa gcaataacat gttagttacg attgatgctg
     6241 ctcaaggtaa aatctttgaa ggatatgcaa acgtactata atttataaaa aaacgtcttt
     6301 ccatttatca acaatggaaa ggcgtttttt ggttcatctg gtattttatg acgtaattaa
     6361 taggttattt gataatgata gtgtatgaat ggcaatctat ataaatgttt atatctttta
     6421 tacatgtaca ttatcacctt caaactttca ctcatattac tttggaaatt tattataaaa
     6481 tagaagtatg gatgtatttc tgaaatgata cattattaaa tagatgagaa agtaaaagtt
     6541 ttgagccaag tacgcaattt aatattataa gttgcatata aaaacccctg ttgaacttta
     6601 gtatattcaa caggggtaaa acttgttgtt atttatttga tttagcagcg atacgtttat
     6661 atcttaagta cataaatgct aaaagtataa accaaatcgg aataaaataa attgcacgtc
     6721 ttgtatcaac attaataaat aataacccga acacaaaaat gaagaataca aatattaagt
     6781 agcccatata tttaccacct aatagtttgt acgtagcatt tttatgtaga tctgggtttt
     6841 tacgactata attgatatat gcaatgataa tcagacccca tacaactaaa aataacactg
     6901 tagagatggt agtcacatac gtaaatactt ttgtcgcatc tgggaaaata tagtttagta
     6961 atgctgccac aagtaataat gctgatgaag caaagattgc aacatgtgga acgccatatt
     7021 tattcgtctt agaaaagttc ggaggtgctt gttgttgact tgataaaccg aaaagcatac
     7081 ggctatttga gaatatacca ctattacatg atgaagcagc agcggttaat actacaaaat
     7141 taatcaagcc cgcagcaaac ggaataccga tcaatgcgaa taattttacg aatggactgt
     7201 tatcaggatc aacttgctgc caagggataa tagacatgat aaccgctaac gccccaacgt
     7261 aaaatattaa aattctaatg ggtacactgt taattgcttt tggaattgtt ttaactggat
     7321 cttttgtttc cccagctgta acaccaatca tttcaattcc tacaaatgag aatagtgcca
     7381 tttggaaaga cataaagaat ccagaagcac ctttagcgaa tatgccgtgt tcatataaat
     7441 ttgtgaaact ggcatgcccg aattgagttt taaatgcaaa tagaatcatg acgaaaccaa
     7501 ctactattaa accaataatt gtcgctattt taatgataga gaaccaaaat tctaattctc
     7561 cgaaaagtct tgcgctaagt aggttgaatg acattaataa cagtacacaa aatagtgcac
     7621 ttatccagtt tggaatttct gggaaccaaa agctaacata ttttgccaca gccgttactt
     7681 cagccatacc tgtaataatc caacagaacc agtatgtcca tccggtaaca aatcctgcaa
     7741 aaggcccaat atatgtattg gttacatctg cgaaagattt aaattcagta ttctgtataa
     7801 tgatttctcc taaacctcgc ataaacataa ataacataaa tcctataatg atgtatgtta
     7861 atagaattga agggccggtt aatgcaatcg tttgaccagc acctaagaat aagcctgtac
     7921 caattgcacc gccaattgca attaattgta tgtggcgatt gctcagttcc ctttgtaatt
     7981 tttcagccat aatacatctc ccttaaatat agatatgttt attatgcact tatattgaga
     8041 tatatacaat tattttcggt aaaaatgtgt aaaattccat gttaatatac tttggttttt
     8101 ataatcatat ataataacca attgaaaatt taattctatt gtaaaattca tggattattc
     8161 acatcttgaa aaagctttaa tggtgctatt tgtggctatt ctgtgacatt tacatagatt
     8221 tacaaaaaat tgttgcacat ataatgccag tttttatatt tcacaaacga aatgcgttta
     8281 gctataatat tagttgaaag ccatttcata aagaaacagt aaaggggaaa tttatcatgg
     8341 cagaattaca aagaggttta gaaggggtta tcgcagcgga gactaaaata agttcaatta
     8401 ttgaaagtca attgacttat gccggctatg atattgatga tctagctgaa aatgcgcaat
     8461 ttgaagaagt tattttccta ttatggaact atagattgcc aaacgaagaa gagcttgctc
     8521 atctaaaagg gaaattaaat caatacatga cattaaatcc tcgtgtgtat acacattttg
     8581 aggagtatgt tacagatcac gtgcatccaa tgacagcatt acgtacgtca ttatcatata
     8641 ttgcacattt tgatcctgat gctgaaaatg aatcagatga aaatcgttat gaaagagcaa
     8701 tgcgtataca ggctaaagta gcatcattag ttacagcgtt tgctcgagta agacaagata
     8761 aagaaccact taagcctaat cctgacttaa gttatgcggc aaacttccta tatatgttac
     8821 gtggggaatt accaacagat atagaagtag aagccttcaa taaagcactt attttacacg
     8881 ctgatcatga gttgaacgca tctgcattta cagcacgttg tgcggtatca tcattgtcag
     8941 atatgtactc aggtattgta gcagctgtag gttctctgaa agggccatta catggtggtg
     9001 caaacgaaca agttatgacg atgttatctg agattgggtc aattgaaaat gttgatgctt
     9061 acttagatga aaaatttgct aataaagata aagtaatggg cttcggtcat cgtgtatata
     9121 aagatggtga tcctagagcg aaatatttaa gagaaatgag ccgtcaaatt acgaaagacg
     9181 ctggtcgtga agaattattt gaaatgtcag tgaaaatgga aaaacgtatg gcagaagaaa
     9241 aaggattaat tcctaatgtt gatttttata gtgcgagtgt ttatcactgt atggaaatac
     9301 ctcatgactt attcacgcca atctttgctg taagtcgttc tgcaggatgg attgctcata
     9361 ttttagaaca atataaagat aatagaatta tgcgtcctag agcgaaatat attggcgaaa
     9421 cgaatcgtaa gtatatcccg cttgaagaaa gaaaataatc aatacaaatt aaaaatgaag
     9481 atgtaaaatt tggaggtaaa ataactatga ctgcagaaaa aattactcaa ggaactgaag
     9541 gattaaacgt acctaatgaa ccaattatcc catttattat cggtgatgga attggaccgg
     9601 atatttggaa ggcagcaagc cgagttatag atgctgctgt tgagaaagcc tataatggcg
     9661 aaaaacgcat tgaatggaaa gaagtgctag ctggccaaaa agcatttgat acaactggtg
     9721 aatggttacc tcaagaaaca cttgatacaa ttaaagaata tttaattgct gttaaaggac
     9781 ctttaacaac accaattggt ggtggtatta gatcattaaa tgtggcttta cgccaagaat
     9841 tagatttatt tacttgttta agaccggtac gttggtttaa aggagtacca tcacctgtta
     9901 aacgtccaca agatgttgat atggttattt tccgtgaaaa tactgaagac atttatgctg
     9961 gtattgaatt taaagaaggt acaacagaag ttaaaaaggt aattgacttc ttacaaaacg
    10021 aaatgggtgc gacaaatatt cgattcccag aaacttcagg tattggtatt aaaccagttt
    10081 ctaaagaagg aactgagcga ttagttagag cagctataca atatgctatc gataataacc
    10141 gtaaatcagt tactttagtt cataaaggta atattatgaa atttacagaa ggctcattta
    10201 agcagtgggg ttacgattta gcattatctg aatttggtga tcaagtattc acttggcaac
    10261 aatatgacga aattgttgaa aaagaaggca gagatgctgc taatgctgct caagaaaaag
    10321 ctgaaaaaga aggcaagatt atcattaaag attctattgc tgacattttc ttacaacaaa
    10381 ttttaactcg tccagctgag catgatgttg tagcaactat gaacttgaat ggtgactata
    10441 tttcagatgc tttagctgca caagttggtg gtattggtat tgcgccaggt gcaaacatta
    10501 attatgaaac aggtcatgct atttttgaag caacacatgg tacagctcca aaatatgcag
    10561 gtttaaataa agtgaatcca tcttcagtaa ttttaagttc tgtattaatg ttagaacatt
    10621 taggatggca agaagcggca gataagatta cagattcaat tgaagataca attgcttcaa
    10681 aagttgttac ttatgacttt gcccgtttaa tggatggtgc tgaagaagtt tctacatcag
    10741 catttgcaga tgaattgatt aaaaatttaa aataagcaaa ataaaaatag gtgagtctag
    10801 caactttaat tgctaggttc gcctttttaa atgaattgaa gttggcaaca ggatagaaat
    10861 aataaagagt aactaatgat tgattatata gtgtttctta cacattagac aaagcgattg
    10921 tgtacataag cttaggaata aatgagtaaa actcatgcat aggtagtggc atagaaatga
    10981 tattttcaaa aagtttattt cgttgtccca gagtaagtca tgcaaatgat agaaggaaat
    11041 ataagaagta ttttatttat atgtaaagta atgtaaaaaa tgtgtaaaga taatgtgtag
    11101 gatgttattt ttataaatag ttcggttata atgtaaatat aattataagt tagggaggca
    11161 taccatgtcg caaaaagtgt tggtagtaga tgacgaacat tcaatcgtaa cattacttaa
    11221 atataactta gaaacagctg gttatgaagt tgttgtcgca tttgatggtg atgaggcttt
    11281 agaaaaggta gaaagtgaac agccagattt aattatttta gatgttatgc tacctaaaaa
    11341 agatggcatt gacgtatgta agactgtaag aacaaataaa aatttagtcc ctatattaat
    11401 gttaacggcg aaaaatgatg aatttgatcg ggtattaggt ttagaattag gtgccgatga
    11461 ctatatgact aagccttttt cacctagaga agttgttgcc cgtgtgaaag ccattttaag
    11521 acgttctcaa tttgtaaaag aagtagaaga ggttgatgat gatattacca tcggatcgat
    11581 acgtattaga cccgattatt ttgaagtata taagcataat gagttattgg agctaacacc
    11641 gaaagaattt gaactcttgc tttatttgat agaaagacaa ggcagagtca ttacgagaga
    11701 acatatgtta aatacggtat ggaattatga atttgcagga gactcaagaa ttgtcgatgt
    11761 acatatcagt catttaagag ataaattaga agataatcct aagaagccac aacttattaa
    11821 aactgtgcgt ggcttaggct ataaattgga aagacctaaa gaacaatgat gaagtttcac
    11881 caccgcttaa tgttattgat aagcagtata ttaattatca gttttgttac attaggcatt
    11941 attattcacg cttccatcac atatgtttta tcagaaaaac acgaacaagc tctagcaaca
    12001 gatgcaagat catatattta tttagtccaa gataatgaga ttgataaagt taaagaaatt
    12061 gtaaaacagc aaaatataga tttattgata actaagcatg ataaaaaggt gtttagtagt
    12121 ggcaaactga aagctatccc aaatagtgat agctatattc aaaaaaggga cttttttatt
    12181 tttaataaga aggttaacgg ttatcatgta tggattaaag gttacaataa cgatattaca
    12241 gaaatgcatt ggacgttgtg gaaatatctt attttgacat gtttagttgt actcatttgt
    12301 ctgtattttg caagccgtag ttttaagcgt acattaatcc gtcccattca agaagttact
    12361 tatgcaacac aattgttggc aaatggttat tatcacatac gtgtacctga aagtaatgtt
    12421 gtagaaacta aagcgctttt tgtgtctacc aatgatttag cacgacgcct tcaaaagctt
    12481 aataatgagc aaaaaattca atccaaccgt ttaaaaacaa cgattgaaaa tattccaagt
    12541 gctattttaa tgatagatcg aaatggaaaa atcgttgttg caaataaagc gtattatgaa
    12601 cagttcaaca tctcgcataa tattgaacaa gtaggctatc acggttatgt taatactgaa
    12661 attgagcaat taattttaga aagttttaaa gttgaaaaac ctatttatga acaattggaa
    12721 gttgcaataa accaagtcca tgccaagtac tttgatattt catgcgttcc gattttaact
    12781 aggtctcaaa aaagtttgca agggatttta gttgtgatgc atgacataac aaatctgaaa
    12841 caacttgaaa atctaagacg tgagtttgta gcaaatgttt cacatgaatt aaaaacacct
    12901 attacttcaa ttaaggggtt tgctgaaacg cttattgatg gtgcaaaaaa tgatgcggaa
    12961 tcattagaca tgtttttaaa cataatttta aaagaatcaa atcgaataga atctttagta
    13021 acagatctgt tagatttatc gcatattgag caacatacag agttagatac agactatatg
    13081 aatttatcag atttaacgcg gcgtattatt gataatatga tgactcaagc caatcaaaaa
    13141 aatatttcca ttcatactga tattgaaaaa gatgtcattg ttaaagcaca agaaagtaaa
    13201 attgctcaag ttatcacgaa tttgttaacg aatgcaatta actattctta tgaagatgga
    13261 gatattaacg ttcgtgtgta tcgagatgac tttcgtgtca ttttcgaagt acaagatttt
    13321 ggtataggta ttaaattaga agaccaacaa cgtatttttg aaagatttta tagagtagat
    13381 aaagcacgaa gtagagattc tggtgggaca ggtcttggat tatcaattac aaagcatata
    13441 gtagaggccc accaaggcaa tattgaagtg aatagtcaag ttggcaaagg ctcaacgttc
    13501 aaagttattc taaaagatta taaagaataa aaattaaagt ggtaacagcg cgtgtatttt
    13561 tcacgaagtt gttgctgctt ttttatttcg ttcaaatctc tattggtatt aaattagagt
    13621 taatgaagtg gaataaattc ggttgtcaat tttgtcattt gtatatgtcg atttgtaagt
    13681 tataagtaat agattgttgt gaatcaagca attttataat atatttatta taataaagaa
    13741 attttagtaa cgcgcttaaa atagatgctt aattaaaaag taataagttt tatttgaacg
    13801 gaaataaatc ttaatttaaa cacttgaata gacaagtgta atcattcgtc atagaaaaag
    13861 acgaggggcg atttagataa attgttgaaa gtaatattaa ataccttatt tatctaattt
    13921 gattgaggaa acatgcatag gtgaaaatta gaaatacatt aaaaatattg aattaatttt
    13981 tcgacctaca agttgaggaa aatttaacta gatttgagga ttagcaaagt gaaaacaatt
    14041 caattacaca agacaacttt gatattaatt gcgatattat tgttttatac ttttatgggt
    14101 atcttactac cactcatgca cgatgattta caatggttta gcaattataa tactgatatt
    14161 ttaaaagtag gatttgcatc acttaatggt cgctatatcg gaaatatctt tgaaattata
    14221 gctgtacatg taagctggtt acgttggctt tcatatggtc tcattagtat gggcatcatt
    14281 tggatgatta tgcacattac acgttgtaaa gcgtggacaa gctattattt gttagcattt
    14341 tctttaatgt taattttacc aagtgctatt tatgcagata catatggctg gtttgcagga
    14401 ttttataatt atgcgacatc aacactaatt tcactcttta ttatttatta ttgtattaat
    14461 gcaattatat ataaagaaaa gcagccggta agtgttactg tactattcta tgttttatgc
    14521 ttcttcggtc aattatttat ggagaatgtg acattattcc actgtatcat tttaatttta
    14581 gcttttctat atgaatttac tgcaaatcga acacttaat
//
LOCUS       DAMZKC010000058        14548 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.058, whole genome shotgun sequence.
ACCESSION   DAMZKC010000058 DAMZKC010000000
VERSION     DAMZKC010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14548
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.058"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(138..1142)
                     /locus_tag="POB55_001292"
     CDS             complement(138..1142)
                     /locus_tag="POB55_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDI6500262.1"
                     /translation="MIETFKAFVIDKDESGKVTPTFKQLSPTDFPKGDVLIKVHYSGI
                     NYKDALATQDHNAVVKSYPMIPGIDLAGTIVESEAPGFEKGEQVIVTSYDLGVSHYGG
                     FSEYARVKSEWIIKLPDTLTLEESMIYGTAGYTAGLAIERLEKVGMNIEDGPVLVRGA
                     SGGVGTLAVLMLNELGYKVIASTGKQDVSDQLLELGAKEVIDRLPVEDDHKKPLASST
                     WQACVDPVGGEGINYVTKRLNHSGSIAVIGMTAGNTYTNSVFPHILRGVNILGIDSVF
                     TAMKLRQRVWRRLAKDLMPENLHEIKQVITFDELPEQLNKVIKHENKGRIVIDFGVDK
                     "
     gene            1333..1848
                     /locus_tag="POB55_001293"
     CDS             1333..1848
                     /locus_tag="POB55_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6500263.1"
                     /translation="MAGIIKEISKQNASELVELATRTFYDTFGSYYDDKDFDQFFKDN
                     YTVEKFTQEINHVDSFHYFYQEDGANVGYIKMNINSAQTEEMGETYLEVQRIYFLKDF
                     QGGGRGSQLIELAEKIAQEHNKHKIWLGVWEHNPRAQAFYKRHGFKVVGEHHFQTGDV
                     TDTDLIMEKEL"
     gene            2162..2776
                     /locus_tag="POB55_001294"
     CDS             2162..2776
                     /locus_tag="POB55_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500264.1"
                     /translation="MKRLVTGLLALSLFLAACGQDSDQQKDGNKEKDDKAKTEQQDKK
                     TNDSSKDKKDNKDDSKDVNKDNKDNSANDNQQQSNSNATNNDQNQTNNNQANNNQKSS
                     YVAPYYGQNAAPVARQIYPFNGNKTQALQQLPNFQTALNAANNEANKFGSNNKVYNDY
                     SIEEHNGNYKYVFSFKDPNANGKYSIVTVDYTGQAMVTDPNYQQ"
     gene            complement(2930..4603)
                     /locus_tag="POB55_001295"
     CDS             complement(2930..4603)
                     /locus_tag="POB55_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HDI6500265.1"
                     /translation="MIKQINVSNMQKFESQLMKAQSEGYTHVVPYANEIMIYQSMLDA
                     VQLYPKSIVVDYTVDGQYKNDCHYFGQSSINIADWAQNNNYYPNLIYAIQQTLDLIHY
                     YSVETIFDLALLTLLKGDLSIDGHVVFDFKAPIATSASIWETIKTIEDFDMMSQFYLN
                     KMAYIDHHPIPFRNLFIEDSEQLNSPDNWLYSTKFMLPKWLYKIAKQRADNKQLQNFG
                     LYTKQPNVLKDHIVFIGDHHQYIGNSKYLFTYFVKHNPMTACYFVTDDRRGPHFISPN
                     SERADELINSARVVIVENDIPETLQPNGTLIQLHQGTPIMQLFLDSKEPIKNIETPFY
                     RAKRYNRWLQFDYVIHSADDISHFYQTAFPSHQANVLAYGNPKHQYLLQKRNESTTQQ
                     QYKKSFKINDQKPVLLYAPIGLVSAQQLPLSDALFKAYHVVVQGVDEAILPEEALVAP
                     KYLSAQDLILMSDVVITDYSNIIFDAMAIDKTVALYTPNHSQYIESQGVNEDIWRHLS
                     KIWYTDRQLLINNLISQAIPVIKYPQIQHKEQPLESISQLILSKMTSNQ"
     gene            4920..6518
                     /locus_tag="POB55_001296"
     CDS             4920..6518
                     /locus_tag="POB55_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HDI6500266.1"
                     /translation="MLVNTFNPFDNLLLSSLIAAIPIVLFLLCLTVFKMKGIYAAITT
                     LVVTLLIAIPFFKLPVGIASGAVVEGFFQGIIPIGYIVMMAVLLYKITVESGQFLTIQ
                     DSITNISQDQRIQVLLIGFAFNAFLEGAAGFGVPIAICALLLTQLGFNPLKAAMLCLV
                     ANAASGAFGAIGIPVGVVETLKLPGDVSVLGVSQSATLTLAIINFIIPFLLIFIIDGF
                     RGVKETLPAILVVSITYTLTQGLLTVFSGPELADIIPPLLTMLALAVFSKKFQPKHIY
                     RVNKDEEIEPAKAHSAKAVLHAWSPFIVLTVIVMIWSAPFFKNLFLPNGALSSLVFKF
                     NLPGTISEVTHKPLVLTLNIIGQTGTAILLTIIITILMSKKVNFKDAGRLFGVTFKEL
                     WLPVLTICFILAISKITTYGGLSAAMGQGIAKAGNVFPVLSPILGWIGVFMTGSVVNN
                     NSLFAPIQASVAQQIGTSGSLLVSANTVGGVAAKLISPQSIAIATAAVKQVGKESELL
                     KMTLKYSVCLLIFICIWTFILSLL"
     gene            6899..8377
                     /gene="mqo"
                     /locus_tag="POB55_001297"
     CDS             6899..8377
                     /gene="mqo"
                     /locus_tag="POB55_001297"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HDI6500267.1"
                     /translation="MTTQHSKTDVILIGGGIMSATLGTLLKELSPEKNIKVFEKLAQP
                     GEESSNVWNNAGTGHSALCELNYTKEGKDGTVDCSKAIKINEQYQISKQFWAYLVKTG
                     QLDNPDRFIQAVPHMSFVIGEDNVAFIKSRVATLKKSVLFEKMKLSQDEEEMKSWVPL
                     MIEGRKSDEPIALTYDETGTDVNFGALTAKLFENLEQRGVGIQYKQNVLDIKKQKSGA
                     WLVKVKDLETNETTTYESDFVFIGAGGASLPLLQKTGIKQSKHIGGFPVSGLFLRCTN
                     QEVIDRHHAKVYGKAAVGAPPMSVPHLDTRFVDGKRSLLFGPFAGFSPKFLKTGSHMD
                     LIKSVKPNNIVTMLSAGIKEMSLTKYLVSQLMLSNDERMDDLRVFFPNAKNEDWEVIT
                     AGQRVQVIKDTEDSKGNLQFGTEVITSDDGTLAALLGASPGASTAVDIMFDVLQRCYR
                     DEFKGWEPKIKEMVPSFGYRLTDHEDLYHKINEEVTKYLQVK"
     gene            complement(8443..8898)
                     /locus_tag="POB55_001298"
     CDS             complement(8443..8898)
                     /locus_tag="POB55_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HDI6500268.1"
                     /translation="MKNLKNSLFISLIIGLSLSLFFSMLFADGKYYPLNPQSTIGILY
                     YTHFTETTVMLISIILWLLIGVVFFLGDFIFKYTDWSITKATIMHFITTYVGFLPLAM
                     LAGWFPLTVHYLIIFTIIFIVVYVLIWIIQFFKNKNYVNTINKQLKQLK"
     gene            complement(8895..9338)
                     /locus_tag="POB55_001299"
     CDS             complement(8895..9338)
                     /locus_tag="POB55_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDI6500269.1"
                     /translation="MMKLNLFINAKETESYIDIHAPKMNDHVQSIINAVNDLDKSHTL
                     VGYIDKEIHIINVSDVITFQVINKNVTAITSNQKFKLKLRLYELEKQLPQHFIRISKS
                     EIVNKYYIEKLLLEPNGLIRMYLKDAHYTYSSRRYLKSIKERLSI"
     gene            complement(9501..10874)
                     /gene="hssS"
                     /locus_tag="POB55_001300"
     CDS             complement(9501..10874)
                     /gene="hssS"
                     /locus_tag="POB55_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme sensor histidine kinase HssS"
                     /protein_id="HDI6500270.1"
                     /translation="MFKTLYARIAIYSITVILFSALISFVLTNVYYHYNLKASNDAKI
                     MKTLKEARQYEQSAKPTHIQQYFKHLGQMNYQIMTVDQKGHKTFYGEPFREDTLSQNA
                     INNVLNNKDYHGIKDKPFALFVTGFFDNVTDNTVGINFKTKDGSIAVFMRPDIGETFS
                     EFRTFLAVLLMLLLFISISLVIASTYSIIRPVKKLKLATERLIDGDFETPIKQTRKDE
                     IGTLQYHFNKMRESLGQVDQMRQHFVQNVSHEIKTPLTHIHHLLSELQQTSDNTLRQQ
                     YINDIYTITTQLSGLTTELLLLSELDNHQHLLFDDKIQVDQLIKDIIRHEQFAADEKS
                     LIILADLESINFLGNQRLLHQALSNLLINAIKYTDVGGAIDIALQHSHNNIIFTISND
                     GSPISPQAEARLFERFYKVSKHDNSNGLGLAITKSIIELHHGTIQFTQSNEYVTTFTI
                     TLPNNSL"
     gene            complement(10867..11541)
                     /gene="hssR"
                     /locus_tag="POB55_001301"
     CDS             complement(10867..11541)
                     /gene="hssR"
                     /locus_tag="POB55_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding heme response regulator HssR"
                     /protein_id="HDI6500271.1"
                     /translation="MVQCLVVDDDPRILNYIASHLQTEHIDAYTQPSGEAALKLLEKQ
                     RVDIAVVDIMMDGMDGFQLCNTLKNDYDIPVIMLTARDALSDKERAFISGTDDYVTKP
                     FEVKELIFRIRAVLRRYNINSNSEMTIGNLTLNQSYLELQVSNKTMTLPNKEFQLLFM
                     LAARPKQIFTREQIIEKIWGYDYEGDERTVDVHIKRLRQRLKKLNATLTIETVRGQGY
                     KVENHV"
     gene            11677..12732
                     /locus_tag="POB55_001302"
     CDS             11677..12732
                     /locus_tag="POB55_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6500272.1"
                     /translation="MKLAIKEIMFYKFRYILITLIILLLSIMVLFISGLAQGLGRENI
                     SLFEHFDNDEYVVQKMKEPQIEKSQLSDTQQNQIKKVIHQEPYKMNIQTLKLSNKEQD
                     VITMNDVKQQRIQLKKGDYPKNAHEVAINDKLAADNIRVGDRLHFKNNSTSYRVSGIL
                     NDTMYAHSSIVLLNDNGFNALNKVNTAFYPVKNLTQQQRDELNKINDVQVVSEKDLTG
                     NIASYQAEQAPLNMMIVSLFAITAIVLSAFFYVMTIQKISQIGILKAIGIKTRHLLSA
                     LVLQILTLTIIGVGIAVIIIVGLSFMMPVTMPFYLTTQNILLMVGIFILVAILGASLS
                     FIKLFKVDPIEAIGGAE"
     gene            12732..13397
                     /locus_tag="POB55_001303"
     CDS             12732..13397
                     /locus_tag="POB55_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6500273.1"
                     /translation="MALVVEDIVKNFGEGLSETKVLKGINFEVEQGEFVILNGASGSG
                     KTTLLTILGGLLSQTSGTVLYNDAPLFDKQHRPSDLRLEDIGFIFQSSHLVPYLKVIE
                     QLTLVGQEAGMTKQQSSTRAIQLLKNIGLEDRLNVYPHQLSGGEKQRVAIMRAFMNNP
                     KIILADEPTASLDADRATKVVEMIRQQIKEQQMIGIMITHDRRLFEYADRVIELEDGK
                     ITD"
     gene            complement(13628..>14548)
                     /locus_tag="POB55_001304"
     CDS             complement(13628..>14548)
                     /locus_tag="POB55_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDI6500274.1"
                     /translation="LIFNYTLTLNLTCYILVVISIFLSLIYSIMTYTFPWSMFVTFII
                     ISIIMLFKHRYLFSHTSSRLFIKKLLSFSYKFVLYTSALLFISTIQSPIVKGLSMWLA
                     ILCLSLLLTFICYLVWTVSYRHRSYNKEVDIIIVLGAGIFTEFVTPMLAARLDRALDI
                     YQQQASATKIIVSGGQGPDEPISEALAMQRYLIAHGVDKTDILMESHSINTYTNFLYS
                     KQIINNLYHEAVKIVCVTSQFHVLRALRLAQKLGLSIDGVGSHTPYHFFLHVLIKDYL
                     GVMYQYRLLLTLYCSSSWFICLYAAFIYNS"
BASE COUNT         5012 a         2105 c         2538 g         4893 t
ORIGIN      
        1 ttgtctttgt gtaccgttta gttgaatttt aattatttaa ttcttttatt gattaatttg
       61 tgtaatgttg attaagtaga taatctaaaa gaaaataaaa agacatctcg cataacggga
      121 tgtctttttc atgaatacta tttatctaca ccgaaatcga taacaatgcg ccctttattt
      181 tcatgtttaa ttactttgtt aagttgttct ggaagttcat caaatgtaat aacttgcttg
      241 atctcatgta aattttcagg cattaaatct tttgcgagac gacgccaaac gcgctgtctt
      301 aatttcatag cagtaaatac cgagtcaatt cctaaaatgt ttacacctct taaaatgtga
      361 gggaatacag aattagtata agtattaccg gcagtcatac caataactgc aattgaccca
      421 ctatgattta aacgctttgt aacataatta ataccttcgc caccaacagg gtctacacaa
      481 gcttgccaag ttgatgatgc gagtggcttt ttatgatcat cttcaacagg aagtcgatcg
      541 ataacttctt tggcaccaag ttcaagtaat tgatcgctaa catcttgttt acctgtactt
      601 gcgataactt tataaccaag ttcattaagc atgagtactg ctaaagtacc gacaccacct
      661 gaagcaccgc gaacgagtac aggaccatct tcaatattca ttccaacttt ttcaagtctt
      721 tcaattgcta aaccggcagt ataaccagct gtgccatata tcattgattc ttctaatgtt
      781 aaagtatcag gaagcttgat aatccattct gattttacac gcgcatattc actaaaaccg
      841 ccataatggc taacacctag gtcataactc gttacaatta cttgttctcc tttttcaaag
      901 cctggtgctt cggattcaac aattgttcca gctaaatcta ttcctggaat cataggatac
      961 gattttacga ctgcattatg atcttgagtc gctaaagcat ctttataatt tataccagag
     1021 taatgtactt taatcagcac atctccttta ggaaaatcag taggcgataa ttgtttgaaa
     1081 gttggtgtca ctttaccgct ctcatcttta tcaattacaa atgctttaaa tgtttctatc
     1141 acaacgatac atcctctctt tttctctttt gaaaatacgg ccattttact ggaataaatg
     1201 tatcatttat tgatgttaca cacaatcaat acaatctgta attgatttgt gttaatgtat
     1261 ttttaaatag ttttggctga aaaataacat aagtaatatt ttgattcgat cactgtttat
     1321 tggaggattt atatggctgg gataatcaaa gaaatttcaa aacaaaatgc tagtgaatta
     1381 gttgaattag caactagaac attttatgac acgtttggtt cttactatga tgacaaagat
     1441 tttgatcaat tttttaaaga caattatact gtagaaaaat ttacacaaga gattaaccat
     1501 gtagattcat ttcattattt ttatcaagaa gatggtgcga atgttggtta tataaagatg
     1561 aatattaata gtgctcaaac tgaagaaatg ggggagacct atttagaagt gcagcgcata
     1621 tattttttga aagactttca aggtggcgga agaggttcac aattgataga attggccgaa
     1681 aaaattgcac aagaacataa taaacataaa atttggctag gagtttggga acataatcct
     1741 cgtgctcaag cattttataa acgtcatgga tttaaagtgg ttggtgaaca tcattttcaa
     1801 acaggtgatg taacggatac agatttaatt atggaaaaag agttataatg attgtttttg
     1861 cagcagataa aatgaaatca tagtaataaa tattgtgtat accttgaaat gctaaatgcg
     1921 taatgaattg ttaaagtaaa ataattaaat ttatagaaaa cataaaattc acaaaaatta
     1981 aataataatg aacaaaaatt agggtataat aaaaagaacc ttagattgta gttatggata
     2041 ttgtgataat tcagatcata ttaatgttga cgtctttggt aacaagccat gaaaagatac
     2101 acatggtaga tatgtatttc aagattattc aatgaatatc gaattatagg aggagatatg
     2161 tatgaaaaga ttagttacag ggttactagc attatcatta tttttagctg catgtggtca
     2221 agatagtgac caacaaaaag acggtaataa agaaaaagat gataaagcga aaactgaaca
     2281 acaagataaa aaaacaaatg attcatctaa agataagaaa gataataaag atgatagtaa
     2341 agacgtaaac aaagataata aagataatag tgcaaacgat aaccagcaac aatctaattc
     2401 aaatgcaaca aacaatgacc aaaaccaaac aaataataac caagcgaata ataatcaaaa
     2461 atcaagttac gttgcaccat attatggaca aaatgccgcg ccggttgcac gtcaaattta
     2521 tccgtttaat ggaaataaaa ctcaagcttt acagcaattg ccaaatttcc aaacagcttt
     2581 aaatgcggct aataatgaag caaataaatt tggtagtaat aataaagtgt ataatgatta
     2641 ttctattgaa gaacataatg gcaactataa gtatgtgttt agttttaaag acccaaatgc
     2701 aaatggaaaa tattcaattg taacggttga ttatactgga caagcaatgg ttactgatcc
     2761 aaactaccaa caataatgtt aatataacta tgatgcaagt taaaaaaata aaacggtaaa
     2821 ctctataata tgaattaggg tttaccgttt tttgcgtatt ttaaagtatc aaagtttacg
     2881 ttttatagta cttttaaacc tcattcacag ccactgagaa atttaaacac tattgattag
     2941 atgtcatctt agacaatata agttgtgaaa tagattctaa tggttgttct ttatgttgta
     3001 tttgtggata tttaatcact ggtattgcct gggaaattaa attattaatt aataattgtc
     3061 ggtctgtata ccaaattttt gatagatgac gccaaatatc ttcgttaaca ccttgagact
     3121 caatgtattg actgtgattt ggagtgtata atgctactgt tttgtctata gccatcgcat
     3181 caaatattat atttgagtaa tcagtgataa cgacatcaga cattagaatt aagtcttgag
     3241 cactaagata ctttggtgca acaagcgcct cttctggtaa gatcgcttca tcaacaccct
     3301 gaaccacaac atgatatgct ttgaataaag catctgatag tggtaattgt tgcgcactca
     3361 ctaatccaat aggtgcgtat aataaaactg gtttttgatc atttatttta aacgatttct
     3421 tatactgttg ttgagtagta ctttcgttac gcttttgtag taaatattgg tgtttaggat
     3481 taccataagc caatacattt gcttggtgac taggaaaagc cgtttgataa aagtggctaa
     3541 tatcatcagc agaatgtata acataatcga attgcaacca gcgattatac cttttagcac
     3601 gataaaaagg tgtttctata tttttaatag gctctttact atctaaaaat aattgcatta
     3661 ttggtgtgcc ttgatgtaat tgaattaaag taccgtttgg ttgtaacgtt tcaggtatat
     3721 cattttcaac tataactacg cgtgcgctgt taatcaattc gtcagctctt tcagaattag
     3781 gtgatatgaa atgtggtccg cgacgatcgt cagtaacaaa ataacaggca gtcattggat
     3841 tatgtttaac gaagtaagta aataaatatt ttgaattacc tatatattga tgatgatcac
     3901 cgataaaaac gatgtgatct tttaaaacgt ttggttgctt cgtatacagg ccgaagtttt
     3961 gtaattgttt gttatcagca cgttgctttg ctattttata taaccattta ggtagcataa
     4021 acttggtaga atacaaccaa ttatctggcg aattaagttg ttctgaatcc tcgataaaaa
     4081 ggttgcgaaa aggtatagga tgatgatcaa tataagccat tttattcaaa taaaactgag
     4141 acatcatatc aaaatcttca atagttttaa tagtttccca tattgaagca cttgttgcta
     4201 taggggcttt aaaatcaaaa acgacatgac catcgattga taaatcacct ttcagaagtg
     4261 tcaataaagc taaatcgaaa atcgtttcta cagagtaata atgtattaaa tcaagagtct
     4321 gttgaatcgc atatatcagg ttaggataat aattattgtt ttgagcccaa tcggcaatgt
     4381 tgatcgatga ttgaccgaaa tagtgacaat catttttgta ttgtccatcc acagtataat
     4441 caaccacgat agacttagga tataattgaa ccgcatctaa catcgattga taaatcatta
     4501 tctcatttgc atatggaacg acatgtgtat aaccttcaga ctgagctttc attaattgag
     4561 attcaaactt ttgcatatta gaaacattta tctgtttaat cattgctata acttccattt
     4621 ctcatcgtag tatcttcctt attttagcac ggtcttatgt aaaattcatg aatagtataa
     4681 gttacttcat tacattaagt acaattcatt ttgatgaaca gatttaaaat gaaaaataaa
     4741 acaattaaat aggaaagtga tattgttaat agtctgaaaa aatggtatat tttaattgtg
     4801 aaaaaaatca cattaatctc actgttctgt aaaatgttat cgataacaaa atgctatagc
     4861 cagagcatga ttttaatgaa gcaattacac tactacttta tggaaagaag agtcagccta
     4921 tgttagtaaa tacgtttaac ccatttgata atttattatt atcgtcttta attgcagcaa
     4981 taccgattgt actgttttta ctatgtttaa ctgtttttaa aatgaaaggt atttatgcag
     5041 ctattacaac acttgttgta acattactaa ttgcaatacc atttttcaaa ttgccagttg
     5101 gtatcgcttc tggtgcagta gtcgaaggtt tcttccaagg tatcattccg attggctata
     5161 tcgttatgat ggcagtattg ttatacaaaa ttactgttga atctggacaa tttttaacaa
     5221 ttcaagatag tattacaaat atttcacaag accaacgtat tcaagtttta cttattggat
     5281 ttgcattcaa cgcattttta gaaggtgcag caggatttgg tgtaccaatt gcaatttgtg
     5341 cacttttatt aacacaatta ggatttaatc cattaaaagc tgcgatgtta tgtttagtcg
     5401 caaatgcagc gtctggtgct tttggtgcga ttggtatccc tgtaggtgtt gtagaaacgt
     5461 tgaaattacc tggagatgtt tcagtattag gtgtttctca atcagcaact ttaacattgg
     5521 caatcataaa tttcattatt ccattcttac ttatctttat tattgatggt tttagaggtg
     5581 ttaaagaaac attaccagca attttagtag tttcaatcac atatacactt actcaaggat
     5641 tattaactgt attcagtgga cctgaattgg cagatattat tccaccgtta ttaacaatgt
     5701 tagcattagc agtattttct aaaaaattcc aaccaaaaca catttatcgt gttaataaag
     5761 atgaagaaat tgaacctgca aaagcacatt ctgcaaaagc agtattacat gcatggagtc
     5821 catttattgt attaacagtc attgtaatga tttggagtgc gccattcttt aaaaacttat
     5881 tcttaccaaa tggtgcttta tcatcattag tatttaaatt caacttacct ggaacaatca
     5941 gcgaagttac gcataaacca ttagtattga ctttaaatat tattggacaa acaggtacag
     6001 ctattttatt aactattatt attacaattt taatgtctaa aaaggttaac tttaaagatg
     6061 ctggtagatt attcggcgtt acatttaaag agttgtggtt accagttctt acaatttgtt
     6121 tcatcttagc aatttctaaa atcacaactt atggtggttt aagtgcagca atgggtcaag
     6181 gtatagctaa agcaggtaat gtcttcccag ttctatcacc aatcttaggt tggataggtg
     6241 tgtttatgac aggatcagtt gtaaataaca attcattatt tgcaccaatt caagcttctg
     6301 ttgcacaaca aattggaaca agtggttcac ttcttgtatc tgctaataca gttggtggtg
     6361 tagcggcaaa attgatttca ccacaatcaa ttgcaattgc aactgcagca gtaaaacaag
     6421 ttggtaagga atcagaatta ttaaaaatga cattgaaata cagtgtatgt ttactaatat
     6481 tcatctgtat ttggactttc atcttgtcat tattataaaa aaacgtattt caaaatataa
     6541 atatacagag ggtgagatgt tttctaacat ctcatctttt tttaatggat cattaatgaa
     6601 agaagtttga cattataata atggtagcgc tttatgttaa aatgaatagt gagtaatcag
     6661 caatcaaatt aaattggttg atagctgtta aggtttgtgg ttttgtcttt gtgctatcgc
     6721 acataaagta tataattaaa gtagtttcgt tattataaaa tattaatata catagtagat
     6781 agtaatagag catcaccatg ggaacctatt gagacactta ttgatttaaa gtggtattaa
     6841 tatgtcgtat ttctcgaacg ttccattatt cattttaaaa agggggactg tatttgttat
     6901 gacaacacaa catagcaaaa cagatgtcat cttaattggt ggcggtatta tgagtgcaac
     6961 attaggaaca ttacttaaag aattatcacc tgagaaaaat attaaagtgt ttgaaaaatt
     7021 agcacaacct ggcgaagaga gttcaaatgt atggaataat gccggtacag ggcattcagc
     7081 actttgcgag ttgaactata caaaagaagg taaggatggc acagttgatt gtagtaaagc
     7141 aattaagata aatgagcagt accaaatttc aaaacagttt tgggcatatt tagttaaaac
     7201 aggacaatta gataacccag atcgctttat tcaagcggtg ccacacatga gttttgtcat
     7261 tggcgaagat aatgtagctt ttataaaaag tcgtgttgca acgttaaaga aaagtgtttt
     7321 attcgaaaaa atgaaattat cgcaagatga agaagaaatg aaatcttggg taccgttaat
     7381 gattgaaggt cgtaagtctg atgaaccaat tgctttaact tatgatgaaa ctggtacaga
     7441 tgttaacttt ggtgcgttaa ctgcaaagtt atttgaaaat ttagagcaac gtggtgtggg
     7501 aattcaatat aagcagaatg tattagacat caagaaacag aaatctgggg catggctagt
     7561 taaagttaaa gatttagaaa ctaatgaaac gacaacatat gaatctgatt ttgtatttat
     7621 tggtgctggc ggtgcgagtt taccattact ccaaaagact gggattaaac aatcaaaaca
     7681 tattggtggt ttcccggtaa gtggattatt cctgcgctgt acaaatcaag aagtgattga
     7741 tcgtcatcat gctaaagtgt acggaaaagc agcagtgggt gcgccaccaa tgtcagtgcc
     7801 gcacttagat acacgttttg tagacggcaa gcgttcattg ttatttggtc catttgcagg
     7861 tttctcacct aaatttttaa aaacaggttc acatatggat ttaattaaat cggttaaacc
     7921 aaataatatc gtgacgatgt tatctgcagg tatcaaagaa atgagtctta cgaagtattt
     7981 agtgtcacaa ttgatgttat ctaatgatga gcgtatggat gatttaagag tctttttccc
     8041 aaatgctaaa aatgaagatt gggaagtgat tacagcaggg caacgtgtcc aagtaatcaa
     8101 ggatactgag gattctaaag gtaacttaca atttggtact gaagtcatta cgtcagatga
     8161 tggcacatta gctgcattac ttggtgcatc acctggtgcg tcaacagctg tagatattat
     8221 gtttgatgtt ttacagcgat gctatcgtga tgaattcaaa ggatgggaac caaagattaa
     8281 agaaatggtg ccgtcatttg gttatcgctt aacagatcat gaggatttat atcacaaaat
     8341 taatgaagaa gtaactaagt atttacaagt taaataataa atgaaacggt aatgtctttt
     8401 ttaatgtgat agacattacc gttttttagt tgttaatgaa aatcatttta attgtttcag
     8461 ttgcttgtta atagtgttta cgtagttctt atttttaaag aattgaatta tccaaattaa
     8521 tacataaacc acaatgaaga taattgtgaa tatgattaga taatgcactg ttagtggaaa
     8581 ccaaccggca agcattgcta aaggcaagaa tccgacatac gttgttatga aatgcattat
     8641 agttgcttta gtaatgctcc aatctgtgta tttaaagata aaatctccaa ggaaaaagac
     8701 gacgcctatg agtaaccata aaatgataga aatcaacatt acggtagttt ctgtgaaatg
     8761 cgtataatac aatatgccaa tagttgattg tgggttcagt ggataatatt tgccgtctgc
     8821 aaataacata ctaaagaaca gtgaaaggga caaaccaatg attaagctaa taaataatga
     8881 gtttttcaaa tttttcatat tgataagcgc tcctttatag attttaaata acgtctagaa
     8941 gaataggtgt agtgtgcatc tttaagatac atacgtataa gtccatttgg ctctaataat
     9001 aatttttcaa tgtaatactt gttgacgatt tctgatttgg aaatgcgaat gaaatgttgt
     9061 ggtaactgtt tttctagttc ataaagtcgt aattttagtt tgaatttttg attgcttgta
     9121 atggctgtta catttttatt aataacttgg aatgttataa catctgatac attaatgata
     9181 tggatttctt tgtctatgta tccgactaat gtatgtgatt tgtctaaatc attgactgca
     9241 ttaataatac tttgaacgtg atcattcatt ttaggtgcat gtatatcaat ataagattcc
     9301 gtctcttttg cattgataaa taaattgagt ttcatcatag gttaatgcct ccttcaaaat
     9361 tattaaacca taaatgacca tcgatatatt taaattttgt tgaatggtag aaattaaatg
     9421 ttaagtggct agaaagcgct aatcaatata aaagatacct cctgaaataa aaacagaaat
     9481 gttttttcag gaggtagaga ttaaagtgaa ttatttggca gtgtaatagt aaaggtggtt
     9541 acatactcgt tactttgtgt gaattggatt gtaccatgat gcaattcaat gatggatttt
     9601 gtaattgcaa gacctaaacc attgctatta tcatgtttgc tcactttata aaaacgttca
     9661 aataaacgtg cttcagcttg tggactaatt ggtgaaccat cattacttat tgtaaaaatg
     9721 atgttgttgt gactatgttg caaagcgatg tcaatggcac caccaacatc tgtatactta
     9781 atagcattta ttaataaatt actcaatgct tgatgtaaca aacgttgatt tcctaggaaa
     9841 ttgattgatt ctagatcagc taaaataatt aacgactttt catcagcagc aaattgttca
     9901 tgtcgaatga tatctttaat gagttgatcg acttgaatct tatcatcgaa taataaatgt
     9961 tggtgattat ctaattcgga taagagcaac aattcagttg ttaatccgct cagctgcgtt
    10021 gtaatggtat aaatatcatt tatatattgt tgtcttaatg tattatcaga agtttgttgt
    10081 aactcgctta ataaatgatg aatatgtgtt aatggtgttt tgatttcatg agatacattt
    10141 tgtacaaaat gctgcctcat ttgatcaact tgtcccaatg actctctcat cttattaaag
    10201 tgatactgta atgttccaat ttcatctttg cgtgtttgtt tgataggtgt ttcaaaatca
    10261 ccatcaatta agcgttcggt cgctagcttt aactttttta ctggacgaat gattgaatag
    10321 gttgatgcga taactaatga aatcgagata aataataaca acattaacaa gacggctaag
    10381 aatgttctaa actcgctaaa tgtttctcca atatctggac gcatgaatac tgctattgaa
    10441 ccgtcttttg tcttaaagtt aataccaaca gtattatctg ttacattgtc gaaaaatcca
    10501 gttacgaata atgcgaatgg tttatcttta ataccatggt aatctttatt atttaatacg
    10561 ttgttaatag cattttgtga taaagtatct tctctaaatg gctccccata aaatgtctta
    10621 tgaccttttt gatcaacggt cattatttgg taattcattt gccctaaatg tttgaaatat
    10681 tgttgaatgt gcgttggttt agctgattgt tcatattgtc ttgcttcttt aagtgtcttc
    10741 atgattttcg cgtcattaga tgcttttaaa ttataatgat agtaaacatt tgttaataca
    10801 aaacttatta atgcactaaa taaaataacc gtaatggaat aaatcgcaat tctagcatag
    10861 agtgttttaa acatgattct ccaccttata gccttgtcct cttactgttt caattgtaag
    10921 tgtggcattt aattttttta atctttggcg tagtcgctta atatgaacgt caactgttcg
    10981 ttcatctcct tcataatcat agccccaaat tttttctatt atctgttcac gtgtaaagat
    11041 ttgcttagga cgtgctgcaa gcataaataa taattgaaat tccttgttcg gtaacgtcat
    11101 cgttttatta gatacttgga gttccaaata ggattggttt agcgttaagt tgccaatagt
    11161 catttctgaa tttgaattga tattatatcg acgtaatacc gcacgaattc taaaaataag
    11221 ttccttaacc tcaaagggtt tggttacata atcgtcagta ccgcttataa acgcacgctc
    11281 tttgtcacta agtgcatccc gcgctgttaa cataataact ggtatatcat aatcattttt
    11341 taatgtatta cataattgaa agccgtccat accatccatc ataatatcta ccactgcaat
    11401 atcgacacgc tgtttttcta aaagttttaa tgcagcttca ccacttggtt gtgtgtatgc
    11461 atcaatgtgc tctgtttgta aatggctagc tatataattt aggattcgag gatcgtcatc
    11521 gacaacaaga cattgcacca tagctataaa ctcccttatc tttttcattt attatacatg
    11581 taaaatattt ttgcgtaaaa aaacaattgt tcatattgag ttcatatttc aaccttatac
    11641 tgacgctaaa gaagaaatag ggagaagtga atcgatatga aattagcgat aaaagagatt
    11701 atgttttaca aatttcgtta tattttaatc acattaatta ttcttttatt aagtattatg
    11761 gtgttattta ttagtggttt agctcaaggg cttggtaggg agaatatttc gttatttgaa
    11821 cactttgata atgatgaata tgttgttcaa aaaatgaaag agccgcaaat tgagaaatcg
    11881 caacttagtg atacgcaaca gaatcaaatt aaaaaggtaa ttcatcaaga accatataaa
    11941 atgaatattc aaacattgaa attatctaat aaagaacaag atgtgattac aatgaatgac
    12001 gttaaacaac aacgcataca acttaaaaaa ggtgattatc ctaaaaatgc tcatgaggtc
    12061 gctattaatg ataagttagc tgcagacaac attagagtcg gggatagatt acattttaaa
    12121 aataattcaa ctagttatag agtttctggt attttaaacg acacaatgta tgcgcatagt
    12181 tccattgtgc tattgaacga taacggattt aatgcattga ataaggttaa tacggcattt
    12241 tatccagtga aaaatttaac acaacaacaa cgtgatgagc ttaataaaat aaatgacgtt
    12301 caagtggtga gtgaaaaaga tttaacaggt aatattgcga gttatcaagc agagcaagca
    12361 ccgttaaata tgatgattgt tagtttgttt gctattacag caatcgttct aagtgcattt
    12421 ttctatgtta tgacgattca aaaaatatca caaattggca ttttgaaagc aattggtatt
    12481 aagacaagac atttattgag tgcgttagtt ttacaaattt taacactaac aataattggg
    12541 gtaggtattg ctgtgatcat catagtagga ctatcattta tgatgccggt aacgatgcct
    12601 ttttacttaa caacgcaaaa tattttatta atggtgggga tatttatatt agtagcgatt
    12661 ttaggtgcct cactatcatt tatcaaatta tttaaagtgg atcctatcga agcaattgga
    12721 ggtgcagaat aatggcatta gtcgttgaag atatcgtcaa aaatttcgga gaaggtttgt
    12781 ctgaaacaaa agttttaaaa ggtattaatt ttgaagtgga acaaggggaa tttgtcattt
    12841 taaatggtgc ctctggttct gggaaaacaa cattgctaac gatattaggc ggattgttaa
    12901 gtcaaacgag tggtacagtg ctttacaatg atgcgccatt gtttgataaa cagcatcgtc
    12961 ctagtgattt acgattggaa gatattggtt ttatttttca atcttcacat ttagttcctt
    13021 atttaaaagt gatagagcaa ttgacactcg taggtcaaga agcgggaatg accaaacaac
    13081 aaagttcaac aagagcaata caacttttga aaaatattgg tttagaagat cgcttgaatg
    13141 tatatccgca tcagttatct ggcggtgaaa agcaacgtgt tgcgattatg agagcattta
    13201 tgaataatcc gaaaatcatt ttagcagatg agcccacagc aagtttagat gccgatagag
    13261 caacaaaagt tgttgagatg atacgtcaac aaattaaaga acaacaaatg attggtatta
    13321 tgattacaca cgatcgaaga ttatttgaat atgcagatcg agtgattgaa ttagaagatg
    13381 gcaaaataac tgattagtgg cttgtaaaga cgctaaatgt taatgattta agacatagta
    13441 gtataaaagt tagataacag aatacgattt gggtttacaa aaaagcacgt cactattata
    13501 taaaatgatg acgtgcttta gtttatttat tttcaatata acaattgtct aatcaaggat
    13561 taacgaatat ttaaagatag tttgacgcaa tattagaaac aacctataat aatagtttgt
    13621 ttgtggatta actattataa ataaaagcgg cgtaaagaca tataaaccaa ctacttgaac
    13681 aatataacgt taataacaat ctatactgat acattacgcc tagataatct ttaatgagca
    13741 catgtaagaa aaagtgatat ggtgtatgac ttccgacacc atcgatagat aaacctaatt
    13801 tttgggctag tcgtaaggcg cgtaatacat gaaactgact tgttacacaa acaattttaa
    13861 ctgcttcatg atacaaattg ttgatgattt gtttagaata taaaaagttt gtgtatgtat
    13921 ttatagagtg agattccatt agtatatctg ttttatcaac accatgtgca atcaaataac
    13981 gttgcatagc taaagcttca gaaattggtt cgtctggtcc ttgtccgcca gatacaatga
    14041 tctttgttgc tgatgcttgt tgttgataga tatcaagtgc acggtctaaa cgcgctgcaa
    14101 gcattggtgt gacaaattcg gtaaaaatac cagcacctaa cacaattatg atatcaactt
    14161 ctttgttgta tgatctatgt ctatatgata ctgtccaaac gagataacaa ataaaggtta
    14221 gtaacaggga aagacataat atagctaacc acatagacaa acctttcaca ataggtgact
    14281 gaatcgtact tataaataga agtgctgatg tgtagagtac aaatttatat gaaaaagata
    14341 ataatttttt aataaataag cgactagaag tatgagaaaa taaatatcta tgtttgaata
    14401 gcatgataat actgattatt ataaatgtta caaacataga ccaagggaaa gtataggtca
    14461 tgatgctata gatgagtgac aaaaatatcg atatgacaac taagatgtag catgttaaat
    14521 ttaacgtcag agtatagttg aaaattaa
//
LOCUS       DAMZKC010000059        14488 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.059, whole genome shotgun sequence.
ACCESSION   DAMZKC010000059 DAMZKC010000000
VERSION     DAMZKC010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14488
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.059"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(169..342)
                     /locus_tag="POB55_001305"
     CDS             complement(169..342)
                     /locus_tag="POB55_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NETI motif-containing protein"
                     /protein_id="HDI6500275.1"
                     /translation="MKFKVNENETIADCLSRMKMQGYMPVKRIEKPVFQEQKDGTVEV
                     SHQEIIFVGKKIQ"
     gene            complement(323..925)
                     /locus_tag="POB55_001306"
     CDS             complement(323..925)
                     /locus_tag="POB55_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HDI6500276.1"
                     /translation="MSFVTENPWLMVLTIFIINVCYVTFLTMRTILTLKGYRYIAASV
                     SFLEVLVYIVGLGLVMSNLDHIQNIIAYAFGFSIGIIVGMKIEEKLALGYTVVNVTSA
                     EYELDLPNELRNLGYGVTHYAAFGRDGSRMVMQILTPRKYERKLMDTIKNLDPKAFII
                     AYEPRNIHGGFWTKGIRRRKLKDYEPEELESVVEHEIQSK"
     gene            complement(1196..2017)
                     /gene="nadE"
                     /locus_tag="POB55_001307"
     CDS             complement(1196..2017)
                     /gene="nadE"
                     /locus_tag="POB55_001307"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HDI6500277.1"
                     /translation="MSKLQDVIVQEMKVKKRIDSAEEIMELKQFIKNYVQSHSFIKSL
                     VLGISGGQDSTLVGKLVQMSVNELREEGIDCTFIAVKLPYGVQKDADEVDQALRFIEP
                     DEIVTVNIKPAVDQSVQSLKEAGIVLTDFQKGNEKARERMKVQFSIASNRQGIVVGTD
                     HSAENITGFYTKYGDGAADIAPIFGLNKRQGRQLLAYLGAPKELYEKTPTADLEDDKP
                     QLPDEDALGVTYEAIDNYLEGKPVTPEEQKVIENHYIRNAHKRELAYTRYTWPKS"
     gene            complement(2010..3479)
                     /locus_tag="POB55_001308"
     CDS             complement(2010..3479)
                     /locus_tag="POB55_001308"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HDI6500278.1"
                     /translation="MYQLEDDSLMLHNDLYQINMAESYWNDNIHEKMAVFDLYFRKMP
                     FNSGYAVFNGLKRVIDFIEHFGFSESDLEYLKSIGYKDDFLSYLKDLKFTGSIRSMQE
                     GELCFGNEPLLRVEAPLIQAQLIETILLNIVNFHTLITTKASRIRQIASNDKLMEFGT
                     RRAQEIDAALWGARAAYIGGFDSTSNVRAGKLFGIPVSGTHAHAFVQTYGDEYVAFKK
                     YAERHKNCVFLVDTFHTLKSGVPNAIKVAKELGDKINFVGIRLDSGDIAYLSKEARRM
                     LDEAGFTETKIIASNDLDEETITSLKAQGAKVDSWGVGTKLITGYDQPALGAVYKLVA
                     IENEDGSYSDRIKLSNNAEKVTTPGKKNVYRIINKKTGKAEGDYITLENENPYDEQPL
                     KLFHPVHTYKMKFIKSFEAIDLHHNIYENGKLVYQMPTEDESREYLALGLQSIWDENK
                     RFLNPQEYPVDLSKACWDNKHKRIFEVAEHVKEMEEDNE"
     gene            3663..4739
                     /locus_tag="POB55_001309"
     CDS             3663..4739
                     /locus_tag="POB55_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide synthase oxygenase"
                     /protein_id="HDI6500279.1"
                     /translation="MLFKEAQAFIENMYKECHYETQIINKRLHDIELEIKETGTYTHT
                     EEELIYGAKMAWRNSNRCIGRLFWDSLNVIDARDVTDEASFLSSITYHITQATNEGKL
                     KPYITIYAPKDGPKIFNNQLIRYAGYDNCGDPAEKEVTRLANHLGWKGKGTNFDVLPL
                     IYQLPNESVKFYEYPTSLIKEVPIEHNHYPKLRKLNLKWYAVPIISNMDLKIGGIVYP
                     TAPFNGWYMVTEIGVRNFIDDYRYNLLEKVADAFEFDTLKNNSFNKDRALVELNYAVY
                     HSFKKEGVSIVDHLTAAKQFELFERNEAQQGRQVTGKWSWLAPPLSPTLTSNYHHGYD
                     NTVKDPNFFYKKKESNANQCPFHH"
     gene            4759..5553
                     /locus_tag="POB55_001310"
     CDS             4759..5553
                     /locus_tag="POB55_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HDI6500280.1"
                     /translation="MQLYYLGPKGTFSYLACRQYFSEKETTFQPKSNLFEVIKAVADD
                     DTSIGVVPIENSIEGTINIVADALAQQDVFAHGEIRLDINFALYGNGTDSISDIKKVY
                     SIAPAISQTTNYIHQHQFDYDYVDSTIQSLTKIENGVAAIAPLGSGEAYGFTPIDTHI
                     EDYPHNVTRFLVIKNQQQFDQNATSLMFLITPMHDKPGLLASVLNTFALFNINLSWIE
                     SRPLKTQLGMYRFFVQADSAITTDIKKVIAILETLDFKVEMIGAFN"
     gene            5625..5732
                     /locus_tag="POB55_001311"
     CDS             5625..5732
                     /locus_tag="POB55_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500281.1"
                     /translation="MVDFLLFVLFLHDAAVYVSLPLVQAIANVTFYFSF"
     gene            complement(5743..7305)
                     /locus_tag="POB55_001312"
     CDS             complement(5743..7305)
                     /locus_tag="POB55_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HDI6500282.1"
                     /translation="MAYFNQHQSMISKRYLTFFSKSKKKKPFSAGQLIGLILGPLLFL
                     LTLLFFHPQDLPWKGVYVLAITLWIATWWITEAIPIAATSLLPIVLLPLGHILTPEQV
                     SSEYGNDIIFLFLGGFILAIAMERWNLHTRVALTIINLIGASTSKILLGFMVATGFLS
                     MFVSNTAAVMIMIPIGLAIIKEAHDLQEANTNQTSIQKFEKSLVLAIGYAGTIGGLGT
                     LIGTPPLIILKGQYMQHFGHEISFAKWMIVGIPTVIVLLGITWLYLRYVAFRHDLKYL
                     PGGQTLIKQKLDELGKMKYEEKVVQTIFVLASLLWITREFLLKKWEVTSSVADGTIAI
                     FISILLFVIPAKNTEKHRRIIDWEVAKELPWGVLILFGGGLALAKGISESGLAKWLGE
                     QLKSLNGVSPILIVIVITIFVLFLTEVTSNTATATMILPILATLSVAVGVHPLLLMAP
                     AAMAANCAYMLPVGTPPNAIIFGSGKISIKQMASVGFWVNLISAIIIILVVYYVMPIV
                     LGIDINQPLPLK"
     gene            complement(7510..8607)
                     /locus_tag="POB55_001313"
     CDS             complement(7510..8607)
                     /locus_tag="POB55_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HDI6500283.1"
                     /translation="MYINMKDYGLTGINKTKDTRAIQHALNRGRCKPTTVYIPKGTYD
                     ICKPLTIYGNTTLLLDNETILRRCHSGPLLKNGHRFGFYRGYNGHSHIHIKGGKLDMN
                     GVSYPYNNTAMCIGHAEDIQLIGVTIKNVVSGHAIDACGINGLYIKSCSFEGFIDYSG
                     ERFYSEAIQLDIQVPGAFPKFGTTDGTITKNVVIEDCYFGPSELPEMGSWNRAIGSHA
                     SRHNRYYENIHIRNNIFEDIQGYALTPLKYKDAFIINNKFINCEGGIRYLGVRDGKNA
                     ADVMTGKDLGSQAGINMNIIGNEFKGSMSKDAIHVRNYNNVKHKDVLIVGNTFNNSTQ
                     SIHLEDIDTVFLSPVEAGIQVTTINVDEIKK"
     gene            8979..9539
                     /locus_tag="POB55_001314"
     CDS             8979..9539
                     /locus_tag="POB55_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDI6500284.1"
                     /translation="MTHRALLVVDYSYDFIADDGLLTCGKPGQNIEDFIVSRINDFNY
                     YQDHIFFLMDLHYLHDIHHPESKLFPPHNIVDTSGRELYGKVGKLYETIKAQPNVHFI
                     DKTRYDSFFGTPLDSLLRERSINQVEIVGVCTDICVLHTAISAYNLGYKISVPAEGVA
                     SFNQKGHEWALAHFKNSLGAEVEQHV"
     gene            9592..10521
                     /locus_tag="POB55_001315"
     CDS             9592..10521
                     /locus_tag="POB55_001315"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HDI6500285.1"
                     /translation="MAKTYIFGHKNPDTDAISSAIIMAEFEQLRGNSGAKAYRLGDVS
                     AETQFALDTFNVPAPELLTDDLDGQDVILVDHNEFQQSSDTIASATIKHVIDHHRIAN
                     FETAGPLCYRAEPVGCTATILYKMFRERGFEIKPEIAGLMLSAIISDSLLFKSPTCTQ
                     QDVKAAEELKDIAKVDIQKYGLDMLKAGASTTDKSVEFLLNMDAKSFTMGDYVTRIAQ
                     VNAVDLDEVLNRKEDLEKEMLAVSAQEKYDLFVLVVTDIINSDSKILVVGAEKDKVGE
                     AFNVQLEDDMAFLSGVVSRKKQIVPQITEALTK"
     gene            complement(10716..10889)
                     /locus_tag="POB55_001316"
     CDS             complement(10716..10889)
                     /locus_tag="POB55_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500286.1"
                     /translation="MNVVAVNFKWSFELYMLKVENRAMNRVHNNIYNFNHNEKGRKKT
                     KIYTQHRKYFKKM"
     gene            10941..12320
                     /locus_tag="POB55_001317"
     CDS             10941..12320
                     /locus_tag="POB55_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HDI6500287.1"
                     /translation="MNIIEQKFYDSKAFFNTQQTKDISFRKEQLKKLSKAIKSYESDI
                     LEALYTDLGKNKVEAYATEIGITLKSIKIARKELKNWTKTKNVDTPLYLFPTKSYIKK
                     EPYGTVLIIAPFNYPFQLVFEPLIGAIAAGNTAIIKPSELTPNVARVIKRLINETFDA
                     NYIEVIEGGIEETQTLIHLPFDYVFFTGSENVGKIVYQAASENLVPVTLEMGGKSPVI
                     VDETANIKVASERICFGKFTNAGQTCVAPDYILVHESVKDDLITALSKTLREFYGQNI
                     QQSPDYGRIVNLKHYHRLTSLLNSAQMNIVFGGHSDEDERYIEPTLLDHVTSDSAIMQ
                     EEIFGPILPILTYQSLDEAIAFIHQRPKPLSLYLFSEDENATQRVINELSFGGGAIND
                     TLMHLANPKLPFGGVGASGMGRYHGKYSFDTFTHEKSYIFKSTRLESGVHLPPYKGKF
                     KYIKAFFKN"
     gene            complement(12440..13468)
                     /locus_tag="POB55_001318"
     CDS             complement(12440..13468)
                     /locus_tag="POB55_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HDI6500288.1"
                     /translation="MTNGYIGSYTKKNGKGIYRFELNENQSRIDLLETGFELEASTYL
                     LRNNEVLYGINKEGEQCGVASLKIDDNGELHLLNKCLSSKAGTGCYVSISEDKRYLFE
                     AVYGAGIIRMYELNTHTGEIIRLIQELAHDFPTGTHERQDHPHAHYINQTPDGKYVAV
                     TDLGADRIVTYKFDDNGFEFYKESLFKDSDGTRHIEFHDNGKFAYVVHELSNTVSVAE
                     YNDGKFEELERHLTIPENFDGDTKLAAVRLSHDQQFLYVSNRGHDSIAIFKVLDNGQH
                     LELVTITESGGQFPRDFNIASSDDLLVCAHEQGDSVVTVFERNKETGKITLCDNTRVA
                     SEGVCVIF"
     gene            13738..14139
                     /locus_tag="POB55_001319"
     CDS             13738..14139
                     /locus_tag="POB55_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YolD-like family protein"
                     /protein_id="HDI6500289.1"
                     /translation="MTSYEEETDYRNVPRELLDNNIPMGRGMIKWAPFATLPEQFETI
                     QQYIIDQNKITRPVLSDDQLAELNIRLHEALQYAQPVEVKFYNNGFVDSVRLTIYRID
                     AINYEIDGYVYNQQQRQKISIFDILDIVLLP"
     gene            complement(14157..14365)
                     /locus_tag="POB55_001320"
                     /pseudo
     CDS             complement(14157..14365)
                     /locus_tag="POB55_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4799 a         2574 c         2130 g         4985 t
ORIGIN      
        1 aaatattaag aacccccagc atatgcttta tatagcttgc tgggggtttc tcatacaata
       61 tggatcgagc atcttgcatg tataaacaaa taggctattc aaagtaaaat tactcaaact
      121 ttgaatagcc tttcctcttc aaatagtctt tatttaaatt ggattatgtt attggatttt
      181 cttacctaca aaaatgattt cttgatgtga tacttcaacc gtgccatctt tttgctcttg
      241 aaacacgggt ttttcaatac gttttaccgg catataccct tgcattttca ttcgtgacaa
      301 acaatcagca attgtttcat tctcatttac tttgaatttc atgttctact acactttcca
      361 gttcttctgg ttcataatct ttaagctttc tacgacgaat gcctttagtc cagaatccac
      421 catgtatgtt tcgaggttca tacgcaatga taaatgcttt cggatctaaa ttttttatcg
      481 tatccatcaa tttacgttca tattttcttg gtgttaaaat ttgcatcacc atacgactac
      541 catctctacc aaacgcagca tagtgcgtaa cgccatatcc taaatttcga agttcattcg
      601 gtaaatctaa ctcatattct gctgaagtta catttacaac tgtataacct aatgccagtt
      661 tttcttctat tttcatacca acaatgatac ctattgaaaa accaaatgcg taggcaataa
      721 tattttgaat atggtctaaa ttagacataa ccaaacctaa accaacgata taaactaata
      781 cttctaaaaa actaactgat gcagcaatat aacgataacc tttcaacgtt aaaattgttc
      841 gcatcgttaa aaacgttaca taacaaacgt taatgataaa tatagttagt accattaacc
      901 atggattttc tgttacgaat gacataaggc gccccactct ctttcaatta cactttaaat
      961 accaaactgc ttcaaactaa ccttaatgaa tgcatattaa tataatatag tacgaaaact
     1021 ctcataaata aatagcgaat ttgtcataaa taaaaattta ttctcaaatg aacatactta
     1081 ctttagttca tacactttca atttatacac tgaagtagtc tagctgaatg tggtttaatg
     1141 ttattaattc taactcatat ttaatttaag tcacacgtta gaagaaaaaa ttaaattagg
     1201 attttggcca cgtgtatctt gtatatgcaa gttcacgttt gtgtgcattt cgtatataat
     1261 gattttcaat tactttttgt tcttctggcg taactggctt accttctaaa taattatcaa
     1321 tcgcctcata agttacacct aatgcatctt catctggaag ctgtggttta tcatcttcta
     1381 aatcagcagt tggcgttttt tcatataatt cctttggcgc accaagatac gctaataatt
     1441 gacgaccttg tcgtttattc aaaccaaata taggtgcgat atctgcagca ccatcaccgt
     1501 acttcgtata aaaaccagtt atattttctg cagaatgatc agtgccaact acgatacctt
     1561 gtcggtttga agcaattgaa aattgtactt tcatacgttc acgcgctttt tcatttcctt
     1621 tttggaaatc tgtaagaaca ataccggctt cttttaatga ttgcacactt tgatcaactg
     1681 caggcttaat attgactgtt actatttcat ctggttcaat gaatcgcaaa gcttggtcaa
     1741 cttcatcagc atctttttga actccataag gtaatttaac tgcaataaac gtacaatcaa
     1801 tgccttcttc acgtaattcg ttaacagaca tttgtactag ttttccaact aatgtagaat
     1861 cctgtcctcc tgaaatacct aacactaaag attttataaa tgaatgtgat tgtacataat
     1921 tttttataaa ttgctttaat tccataattt cttcagcact atcgatacgc tttttcactt
     1981 tcatttcttg tacaataacg tcttgtaatt tactcattat cttcttccat ctccttaacg
     2041 tgttccgcaa cttcaaaaat acgtttatgt ttattatccc aacatgcctt gcttaaatcg
     2101 actggatatt cttgtggatt caggaaacgc ttattttcat cccaaataga ttgtaatcct
     2161 agtgctaaat attcacgtga ttcatcttct gttggcattt gatatactaa tttaccattt
     2221 tcataaatat tatgatgcaa atcaatggct tcgaaagatt ttataaattt cattttataa
     2281 gtatgcactg gatggaataa ttttaaaggt tgttcatcgt atggattttc attttccaaa
     2341 gtaatataat cgccttctgc cttacctgtt ttcttgttta taatgcgata tacatttttc
     2401 ttacctggcg tcgtaacctt ttcagcgtta tttgataatt taatacgatc actatatgaa
     2461 ccatcttcat tttcaatagc tacaagttta tatactgcac ctaatgctgg ttgatcgtat
     2521 cctgtaatca gctttgtacc aacgccccaa gaatctactt ttgcaccttg tgctttcaaa
     2581 ctcgtaatcg tttcttcatc caaatcatta gacgcgataa ttttagtttc agtaaatcct
     2641 gcttcatcaa gcatacgtct tgcctcttta gataaataag cgatatctcc agaatctaat
     2701 cgaataccta caaagttaat tttgtcacct aattcttttg caacttttat tgcgtttggc
     2761 acgccagatt ttaaagtatg gaatgtatct actaggaaca cacaattttt atgtctttca
     2821 gcatattttt tgaaggcaac atattcgtct ccataagttt ggacaaatgc atgtgcatgt
     2881 gtaccagaca caggtatacc aaataatttc cccgccctaa cattacttgt agaatcaaag
     2941 cccccgatgt aagcagctct agcgccccac aatgctgcat caatttcttg cgcacgacgt
     3001 gtaccaaact ccattaattt atcatttgat gcaatttgac gaattctgct agcctttgtt
     3061 gtaattagtg tatggaaatt tacaatgttt aataaaattg tttctattaa ttgcgcttga
     3121 atcaatggtg cttctacgcg taacaatggt tcgttaccaa agcataactc gccttcttgc
     3181 atcgaacgaa tgcttcctgt gaattttaaa tcttttaaat atgataagaa atcatccttg
     3241 tagccaatag actttaaata ttccaaatca gattctgaaa atccaaaatg ttctataaaa
     3301 tcaatgacgc gttttaaacc attaaaaaca gcatagccac tattaaatgg catttttcta
     3361 aaatacaaat caaatacagc cattttttca tgaatattat cattccaata actttcagcc
     3421 atatttattt gatataagtc attatgtaac attaaactgt cgtcttctaa ttggtacact
     3481 tgtatctctc caatcgacct aaatattttc ttacatttta tcataattca tttttttata
     3541 tacataagag ctccttaatt tccatacttt taattaaaat caaccaacaa tttaatgaca
     3601 tatacataat ttttaagagt attttaataa tgtagactat aatataaagc gaggtgttgt
     3661 taatgttatt taaagaggct caagctttca tagaaaacat gtataaagag tgtcattatg
     3721 aaacgcaaat tatcaataaa cgtttacatg acattgaact agaaataaaa gaaactggga
     3781 catatacaca tacagaagaa gaacttattt atggtgctaa aatggcttgg cgtaattcaa
     3841 atcgttgcat tggtcgttta ttttgggatt cgttaaatgt cattgatgca agagatgtta
     3901 ctgacgaagc atcgttctta tcatcaatta cttatcatat tacacaggct acaaatgaag
     3961 gtaaattaaa gccgtatatt actatatatg ctccaaagga tggacctaaa attttcaaca
     4021 atcaattaat tcgctatgct ggctatgaca attgtggtga tcctgctgaa aaagaagtta
     4081 cacgcttagc aaatcactta ggttggaaag gaaaaggtac taattttgac gtgttaccac
     4141 tgatttacca attacctaat gagtcagtta aattttacga atatcctact tcattaatta
     4201 aagaagtacc tattgaacat aatcattatc caaaattaag aaaattgaac ttaaaatggt
     4261 atgcagtccc tatcatttcc aatatggact taaaaatcgg tggcattgta tatccaactg
     4321 caccctttaa cggttggtat atggtaactg aaattggcgt acgtaacttt attgatgatt
     4381 accgttacaa tttactagaa aaagttgcag atgcgtttga atttgataca cttaaaaata
     4441 attcatttaa taaagatcga gcacttgttg aattgaacta tgctgtgtat cattccttta
     4501 aaaaagaagg cgtatcaatt gtcgatcatt tgaccgctgc aaagcaattc gaactattcg
     4561 aacgtaatga agcacaacaa ggtcgtcaag ttaccggaaa atggtcttgg ctagcgccgc
     4621 cattatctcc aacattgacg tcaaattatc atcatggata tgacaataca gtaaaagatc
     4681 caaacttttt ctataaaaag aaagaatcaa atgctaacca gtgccctttc catcattaag
     4741 ttagtagagg tgtagcatat gcaattatat tatttaggcc ctaaaggtac attttcatat
     4801 ttggcttgtc gtcagtattt ttctgaaaag gaaacaactt ttcaaccaaa gtcaaattta
     4861 tttgaagtca tcaaggcagt tgcagatgat gacacatcga ttggtgttgt ccctattgaa
     4921 aattctatag aaggaacaat caacattgtg gcagatgcac ttgcacagca agatgtcttt
     4981 gcacacggtg aaatacgttt agatattaac tttgcattat atggtaatgg aacagattcc
     5041 atttctgata ttaaaaaagt gtattctatt gcaccagcaa tcagtcaaac aacaaattac
     5101 atacatcaac atcaatttga ctatgattat gtcgacagta caattcaaag tttgactaaa
     5161 attgaaaatg gcgttgcagc aattgcacct ttaggtagtg gcgaagctta tggttttaca
     5221 cctattgata cacatattga agactatcca cataacgtga cacgtttttt agttataaaa
     5281 aatcagcagc aatttgatca aaatgcgaca tcattgatgt ttttaattac accaatgcat
     5341 gataaacctg ggttgttagc aagtgtgctt aatacatttg ctttgtttaa tattaattta
     5401 tcctggattg agtctcgtcc tttaaaaaca caattaggta tgtatcgatt tttcgttcag
     5461 gctgattctg caattacaac cgatataaag aaagtcattg ctattttaga aaccttagat
     5521 ttcaaagtag aaatgattgg tgcttttaat taaactttat tttctaggtt ctacaattta
     5581 aataaaataa agagtcaacc aaggattaaa ataggaatcc atccttggtt gactttcttt
     5641 tgtttgtatt gtttttacac gatgctgctg tatacgtttc attaccactc gttcaagcaa
     5701 ttgctaatgt aaccttttat ttttcgttct aatctgcaat tactatttca atggcagtgg
     5761 ttgatttata tcaataccta aaactatagg cattacataa tacacgacta aaataataat
     5821 tattgcactg attaagttta cccagaatcc tactgatgcc atttgtttga tagatatttt
     5881 acctgaacca aagataattg cattcggtgg tgtccctact ggtaacatgt atgcacagtt
     5941 agccgccata gctgcaggtg ccataagtag taatggatgc actccaacag caacagacaa
     6001 cgttgctaaa atcggtaaaa tcatcgttgc agttgcagta ttagatgtca cttcagttaa
     6061 aaataagaca aagattgtta tgacaattac aataagaatc ggactaacac catttaatga
     6121 tttcaactgt tcgcctaacc attttgctaa accactttca gaaatacctt tcgctaatgc
     6181 taaaccgcca ccaaataaaa ttaatacacc ccaagggagc tcttttgcaa cttcccagtc
     6241 aatgatacgg cgatgttttt cagtattttt agctggaata acaaataata atattgatat
     6301 aaaaatagca atcgtaccat ctgcaacgga tgacgtaact tcccattttt tcagaagaaa
     6361 ctctcttgta atccataata agctggcaag tacaaagata gtttgtacta ccttttcttc
     6421 atacttcatt ttgccaagct cgtctaactt ttgtttaatt aacgtctgac caccaggtaa
     6481 atatttcaaa tcatgtctaa acgcaacata tcttaaatag agccaagtaa tacctaacaa
     6541 aacaatgacc gttggaatcc ctacaatcat ccatttagca aaactaattt catgtccaaa
     6601 atgttgcata tattgtcctt ttaaaataat taatggcggg gttccgatta atgtacccaa
     6661 gccaccaatc gtacctgcat agccaattgc taaaactaga gatttttcaa acttttgaat
     6721 acttgtttga ttcgtattgg cttcttgtaa atcatgtgct tccttaataa ttgctaaacc
     6781 aatcggaatc ataatcatta cagctgcagt gttcgataca aacatagata agaatcctgt
     6841 tgccaccatg aatccaagta atatttttga agtgcttgca ccaatcaaat taataattgt
     6901 caacgctact ctcgtatgta aattccatct ttccattgca attgccaaaa taaatccacc
     6961 taaaaacaaa aagataatat cattgccata ttcggatgat acttgttctg gtgtaagtat
     7021 atgacctaat ggtaataaca caattggtaa taagctcgtt gctgcaatag gaattgcttc
     7081 agtaatccac caagtcgcaa tccataaagt aatcgctaag acatatacgc ctttccaagg
     7141 taagtcttgt ggatgaaaga ataataatgt taataggaaa agtaatggac ctaatattag
     7201 tccaataagt tgtcccgcac taaacggttt ctttttcttt gattttgaaa agaatgttaa
     7261 ataccttttc gatatcattg attgatgttg attgaaataa gccataaatt tccccctcat
     7321 gatgattatg acaagctact tcaactcact atattagagt atagaaagtt gtctatcttg
     7381 tattatcatt accgtcaaaa tacccctttc atcagataat tgggtttatt atagcatatt
     7441 tataccgttt tatgaatgcg ctttcaaaaa ttatctacta ttcttaatcc taatgtcatg
     7501 cgaaactttt tactttttta tttcatctac attgattgta gtaacttgaa tacccgcttc
     7561 aacaggactt aaaaacactg tatcaatatc ttctaaatga attgattgag tcgaattatt
     7621 gaatgtattc ccaacgatta atacatcttt atgtttaaca ttattataat tacgtacatg
     7681 tatcgcatct ttagacattg atcctttaaa ttcatttcca attatattca tatttatgcc
     7741 tgcttgggaa cctaagtctt ttcctgtcat cacatctgct gcatttttac catctctaac
     7801 tcctaaatat ctaataccac cctcacagtt aataaactta ttattaataa tgaaagcatc
     7861 tttatacttc aagggagtta atgcataacc ttgtatatct tcaaatatat tatttctaat
     7921 atgaatattc tcatagtaac gattatgtct acttgcatgt gagccaatag cacgattcca
     7981 acttcccatt tcgggcaatt ctgaaggtcc aaaataacaa tcttcgataa cgacattttt
     8041 cgttatcgta ccatctgtcg ttccgaattt tggaaaagca ccaggtactt gaatgtctaa
     8101 ttgtattgct tcagaataaa agcgttcgcc actatagtct ataaatcctt caaatgaaca
     8161 acttttaata tagagtccat taatcccgca agcatcaatt gcatgaccac tcactacatt
     8221 cttaatagtc acacctatta attgaatatc ttcagcatgc ccaatgcaca tagctgtatt
     8281 gttataagga tacgatacgc cattcatatc aagcttgccg cctttaatat gaatatgact
     8341 gtgtccatta taaccacgat aaaaaccaaa gcgatgacca ttttttaata aaggaccaga
     8401 atgacatcgg cgtaaaatag tttcattatc caacaaaagt gttgtattgc catatatcgt
     8461 taatggtttg caaatatcat acgtcccttt cggtatataa actgtcgttg gtttacatct
     8521 tccacgattt aacgcatgtt gtattgctcg agtatcttta gttttgttta tgcctgttaa
     8581 cccataatct ttcatattga tatacataaa tcgtcatcct ttgtctattt tctaattact
     8641 catatattaa ccaattattc aaattataat cgtgttaggt tgatttaaca gatagtcata
     8701 aatctcgtga tgccatctat tagcattttt atattcaaaa catcaacact tgatattagt
     8761 tctaagtttg tcacatttag tattctatta cagctatttt aaaactatcg caaaaacttc
     8821 ttatattatt tgttgattgc gtgatttgct cttccaaaaa agttgattaa tctatatact
     8881 taatctatat ttatatatta accattaggg ttaaaaatta ctctagcatt tatgaataga
     8941 tgggagttta ttttattatt atataggaga gatgttgaat gacacatcgc gcactattag
     9001 ttgttgacta ttcatatgac tttatcgcag atgacggctt actaacatgc ggtaaacctg
     9061 gacaaaatat tgaagatttt attgtttctc gtatcaatga ctttaattat tatcaagacc
     9121 atatattctt tttaatggat ttacattatt tacatgacat tcatcatcct gaaagtaaat
     9181 tattcccacc acacaatatc gtagatacaa gtggtagaga attatacggt aaagtaggta
     9241 aattatacga aacaattaaa gcgcaaccta atgtacattt cattgataaa acgcgctatg
     9301 attcgttctt tggtaccccg cttgatagtt tattgagaga aagaagtatt aatcaagtcg
     9361 aaatcgttgg tgtatgtacc gatatttgcg tgttacatac agcaatttct gcatacaact
     9421 taggttataa aatttcagta cctgctgagg gagtggcttc atttaatcaa aaagggcatg
     9481 aatgggcact tgcacatttc aaaaactcat taggtgcaga ggtagaacaa cacgtttaaa
     9541 tcgtgctaaa ataattataa agaatacaat ttacaaggga gatatttgac aatggctaaa
     9601 acatatattt tcggacataa gaatccagac actgatgcaa tttcatctgc gattattatg
     9661 gcagaatttg aacaacttag aggtaattca ggagccaaag cataccgttt aggtgatgtg
     9721 agtgcagaaa ctcaattcgc gttagataca tttaatgtac ctgctccgga attattaacg
     9781 gatgatttag atggtcaaga tgttatctta gttgatcata acgaattcca acaaagttct
     9841 gatacgattg cctctgctac aattaagcat gtaattgatc atcacagaat tgcaaatttc
     9901 gaaactgctg gtcctttatg ttatcgtgct gaaccagttg gttgtacagc tacaatttta
     9961 tacaaaatgt ttagagaacg tggctttgaa attaaacctg aaattgccgg tttaatgtta
    10021 tcagcaatta tctcagatag cttacttttc aaatcaccaa catgtacaca acaagatgtt
    10081 aaagcagctg aagaattaaa agatattgct aaagttgata ttcaaaagta cggcttagat
    10141 atgttaaaag caggtgcttc aacaactgat aaatcagttg aattcttatt aaacatggat
    10201 gctaaatcat ttactatggg tgactatgtg actcgtattg cacaagttaa tgctgttgac
    10261 cttgacgaag tgttaaatcg taaagaagat ttagaaaaag aaatgttagc tgtaagtgca
    10321 caagaaaaat atgacttatt tgtacttgtt gttactgaca tcattaatag tgattctaaa
    10381 attttagttg taggtgctga aaaagataaa gttggcgaag cattcaatgt tcaattagaa
    10441 gatgacatgg ccttcttatc tggtgtcgtt tctcgaaaaa aacaaatcgt acctcaaatc
    10501 actgaagcat taacaaaata atactatatt actgtctaat tatagacatg ttgtatttaa
    10561 ctaacagttc attaaagtag aatttatttc actttcaatg aactgttttt tatttacgtt
    10621 tgactatttt acaacccctt ttcaatagta gtttttattc ctttagctac cctaacccac
    10681 agattagtga tttctataca attccccttt tgtctttaca ttttcttaaa atatttgcga
    10741 tgttgagtat aaatttttgt tttcttccta cctttttcgt tatgattaaa gttataaata
    10801 ttattatgta cacgattcat cgctctattt tcaactttca acatatataa ttcgaaagac
    10861 catttaaaat taacggccac aacattcaaa tcaattaatc gctttttcca aaataatcat
    10921 ataaggaggt tcttttcatt atgaatatca ttgagcaaaa attttatgac agtaaagctt
    10981 ttttcaatac acaacaaact aaagatatta gttttagaaa agagcaatta aagaagttaa
    11041 gcaaagctat taaatcatac gagagcgata ttttagaagc actatataca gatttaggaa
    11101 aaaataaagt cgaagcttat gctactgaaa ttggcataac tttgaaaagt atcaaaattg
    11161 cccgtaagga acttaaaaac tggactaaaa caaaaaatgt agacacacct ttatatttat
    11221 ttccaacaaa aagctatatc aaaaaagaac cttatggaac agttttgatc attgcaccat
    11281 ttaactatcc ttttcaacta gtgttcgaac ctttaatcgg tgctattgca gcaggtaata
    11341 cggcaattat taaaccatct gagttgacac caaatgttgc acgagtgatt aaacgattaa
    11401 tcaatgaaac atttgatgca aattacattg aagttattga gggaggaatt gaagaaacgc
    11461 aaacgttaat tcacttacct tttgactatg tcttctttac aggaagtgaa aatgtaggca
    11521 aaatcgttta tcaagctgcc agcgaaaatt tagttcctgt gacattagaa atgggtggta
    11581 aatctccagt catcgttgat gaaacagcga atattaaagt tgctagtgag cgcatttgtt
    11641 ttgggaaatt cactaatgcc ggccaaacat gtgttgcacc agattacatt ttagtacacg
    11701 aatctgtaaa agatgattta atcacagccc tatcaaaaac gttgcgtgaa ttttatggtc
    11761 aaaatataca acaaagtcca gattatggcc gcattgtaaa ccttaaacat tatcatcgtc
    11821 tgacttcatt acttaacagt gcacaaatga atattgtatt tggtggtcat agtgatgagg
    11881 atgaacgtta tatagaacca acattgttag atcacgttac aagtgattca gcaattatgc
    11941 aagaagaaat ttttggtcct atcttaccga ttttaacgta tcagtcattg gatgaagcaa
    12001 tagcctttat tcaccaaaga ccaaaacctt tgagtttata tttatttagc gaagatgaaa
    12061 atgctacaca acgtgtaata aacgagctat catttggcgg cggcgctatt aatgatacat
    12121 tgatgcacct agcgaatcct aaattaccat ttggtggtgt tggcgcctca ggtatgggac
    12181 gctatcatgg taaatattca ttcgacactt ttacacatga aaaaagctac attttcaaat
    12241 ctacacgatt agaatcaggt gtccatttac caccatataa aggtaaattt aaatacatca
    12301 aagctttctt taaaaattaa tttttgagcc aaaaataaac catgcttcca taatttgatg
    12361 ttcttgaaaa caataaattt caaggtatac acaaattttt tggaaacatg gttttctttt
    12421 tatcatgatt aattaaagat taaaatatga cacatacacc ttcagatgct acacgagtgt
    12481 tatcacatag cgtaatttta cctgtttctt tatttctttc gaaaacagtt acaactgaat
    12541 ctccttgctc atgagcacaa actaaaaggt catctgatga ggcaatatta aaatctcttg
    12601 ggaattgacc accactttca gtaattgtta ctagttctaa gtgttgacca ttatcaagaa
    12661 ctttaaaaat tgcaatgcta tcatgccctc tattagatac atataagaat tgttgatcat
    12721 gagataaacg cactgctgca agtttagtat ctccatcaaa gttttcagga attgttaaat
    12781 gacgctcgag ctcttcaaat ttaccgtcat tatattctgc aacactcaca gtatttgata
    12841 attcgtgtac gacataagca aattttccat tatcatgaaa ttcaatatgt cttgtcccat
    12901 cactatcttt aaataaagat tctttataaa attcaaaccc gttgtcatca aatttataag
    12961 taacgatacg atcagcacct aaatctgtta ctgcaacgta cttaccatct ggagtttgat
    13021 taatataatg tgcgtgtgga tgatcttgtc tttcatgtgt acctgttgga aaatcatgcg
    13081 caagttcttg aattagacgt ataatttcac ctgtgtgcgt gtttaattca tacatacgta
    13141 tgatgccagc accatatacc gcttcaaata aatatcgttt atcttctgaa atcgatacat
    13201 aacaacctgt accagctttt gaagacaaac atttatttaa taaatgcaat tcaccattgt
    13261 catcaatttt caaactcgca acaccgcatt gttctccttc tttgttgatt ccatataaaa
    13321 cttcattatt acgcaacaaa tatgtagacg cttctaattc aaatcctgtt tctaataaat
    13381 caatacgtga ctgattttcg tttaattcaa agcgataaat ccctttacca ttctttttag
    13441 tgtaagaacc aatatatcca tttgtcatta cttttacccc tccatgttag tcttattttc
    13501 attttattaa attttagcta gaattaacaa tcatttatca tttccttaaa gctaaaacta
    13561 tttaactact ttaaaagaac tatttgctgt gatatgtcat tattcgattt taattaatcc
    13621 ctctttttta cttacacgag cattaattat taacgcgttt atgcttccac aaaatttttg
    13681 ttgcaaaata gaacacatgt tcgtataatg taattatcag attggggtga attaaggatg
    13741 acaagttatg aagaagaaac tgattatcgt aacgttccaa gagaattact agataacaat
    13801 atacctatgg gaagaggcat gattaaatgg gctccttttg caacattacc cgaacaattc
    13861 gaaacgattc aacaatacat tatcgaccaa aacaaaatta cacgtcctgt attatcagat
    13921 gatcaattgg ctgagctaaa cattcggcta cacgaggcgc tacaatatgc ccaacctgta
    13981 gaagttaaat tttataataa tggctttgta gattcagtac gcttaaccat ttatcgtatt
    14041 gatgctatta attatgaaat tgatggttat gtttacaatc agcaacaacg tcaaaaaatt
    14101 tctatctttg atatcttaga tattgtcttg ttaccttaat gctttgaaca taattaaaca
    14161 gattgagtat atgcatataa gatgattttt aacatcattt ttggatgata ggatgttgcg
    14221 ccacgatgat gtctgaattc atcgaattcg ctatcaggta ttgtttcaac aatttcatta
    14281 acatatcgtg aaatatcatt ttgagggatt ctaacagaag tttctattgg tagtgtagtt
    14341 gggtcatgtt ataattttta tacataaggc acctcgttaa tttagtttag tggtatttat
    14401 taaattataa gaagggaccc aacacagaaa attcatttta ttgaatttta catttatgtg
    14461 caagttgggc aaagtgtctt atttttta
//
LOCUS       DAMZKC010000060        14445 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.060, whole genome shotgun sequence.
ACCESSION   DAMZKC010000060 DAMZKC010000000
VERSION     DAMZKC010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14445
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.060"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(140..700)
                     /locus_tag="POB55_001321"
     CDS             complement(140..700)
                     /locus_tag="POB55_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HDI6500290.1"
                     /translation="MNTIRNSICLTIITMVLCGFLFPLAITLIGQIFFYQQANGSLIT
                     YDNRIVGSKLIGQHWTETRYFHGRPSAVDYNMNPEKLYKNGVSSGGSNESNGNTELIA
                     RMKHHVKFGNSNVTIDAATSSGSGLDPHITVENALKQAPRIADARHISTSRVADLIQH
                     RKQRGVLTNDYVNVLELNIALDKMKD"
     gene            complement(720..2747)
                     /gene="kdpB"
                     /locus_tag="POB55_001322"
     CDS             complement(720..2747)
                     /gene="kdpB"
                     /locus_tag="POB55_001322"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HDI6500291.1"
                     /translation="MHHVNKYFNQTMVIEALKMSFYKLNPKQLIKNPIMFVVEVGMIL
                     TLILICFPDIFGTSYLSRGYLITIFIILLITILFANFSEAFAEGRGKAQADSLRQAQS
                     NLTARLIEENGAYRIVNATELKAGQNIRVENGETIPADGVVINGLATVDESAITGESA
                     PVIKESGGDFDGVIGGTLVTSDWLEIRVESEAGTSFLDKMIALVEGAERNKTPNEIAL
                     FTLLTTLTIIFLVVIVTLYPIASYLHLILPIAMLIALTVCLIPTTIGGLLSAIGIAGM
                     DRVTQFNVLAKSGRAVEVCGDVDVMILDKTGTITYGNRIASEFLPVNQQMLEKLIVAA
                     YMSSIYDDTPEGKSIVRLAKQMYINELPKDIDGTYKTFTAETRMSGIITNEISVFKGA
                     PNSMINLVKQQQGNIPLNIESLCMDVSSKGGTPLIVIENNVMLGVIYLKDVIKDGLVE
                     RFTELRKMGIETVMCTGDNALTAATIAKEAGVDRFVAECKPEDKIKVIKDEQAKGHIV
                     AMTGDGTNDAPALAQANIGLAMNSGTISAKEAANLIDLDSNPTKLIEVVKIGKQLLMT
                     RGALTTFSLANDVAKYFAILPALMMSTIPEMTSLNIMHLSSPKSAIISALIFNALIIV
                     ALIPIAMKGVKVKGYSIDRIFINNMLIYGLGGLIVPFLGIKLIDMIVQFFV"
     gene            complement(2766..4442)
                     /gene="kdpA"
                     /locus_tag="POB55_001323"
     CDS             complement(2766..4442)
                     /gene="kdpA"
                     /locus_tag="POB55_001323"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit A"
                     /protein_id="HDI6500292.1"
                     /translation="MEIILFLTMMVMITYVFSGYLYRVALVQSSRVDLIFTRFENMCF
                     KIIGTDLEHMSAKTYVKHFLAFNGFMGFITFVLLIVQQWLFLNPNHNLNQSIDLAFNT
                     AISFLTNSNLQHYNGESDVTYLTQMIVMTYLMFTSSASGYAVCIAMLRRLTGLTNIIG
                     NFYQDIVRFIVRVLLPLSCLISILLMTQGVPQTLHANLMIRTLSGHIQHIAFGPIASL
                     ESIKHLGTNGGGFLAGNSATPFENPNIWSNFIEMGSMMLLPMSMLFLFGRMLSRHGKR
                     VHRHALILFVAMFFIFIAILTLTMWSEYRGNPILANLGIYGPNMEGKEVRFGAGLSAL
                     FTVITTAFTTGSVNNMHDSLTPLGGLGPMVLMMLNVVFGGEGVGLMNLLIYVLLTVFI
                     CSLMVGKTPEYLNMPIGAREMKCIVLVFLIHPILILVFSALAFMIPGASESITNPSFH
                     GISQVMYEMTSAAANNGSGFEGLKDDTTFWNISTGIIMLLSRYIPIILQLMIASSLVN
                     KKSYHQDKYTIAIDKPYFGVSLIVFIVLLSGLTFIPVLLLGPIGEFLTLK"
     gene            complement(4466..4576)
                     /gene="kdpF"
                     /locus_tag="POB55_001324"
     CDS             complement(4466..4576)
                     /gene="kdpF"
                     /locus_tag="POB55_001324"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HDI6500293.1"
                     /translation="MLLCCKEVMTMLIVLLLVVIALVLYLFYALIRSEKF"
     gene            4714..7371
                     /locus_tag="POB55_001325"
     CDS             4714..7371
                     /locus_tag="POB55_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HDI6500294.1"
                     /translation="MSNTESLNIGKKRGSLTIYIGYSPGVGKTFEMLSNAIELFQSNV
                     DIKIGYIEPHQRDETNALAEQLPKITTNFTKHGSHHFQYLDVDRIIEESPTIVLIDEL
                     AHTNISRDRHEKRYMDIEEILNHGIDVHTTLNIQHIESLSSQIELMTGIHVKERVPDY
                     FIMSADVLEVVDISPEQLIKRLKAGKVYKKDRLDVAFSNFFTYAHLSELRTLTLRTVA
                     DLMSDKEKVRHNHKTSLKPHIAVAISGSIYNEAVIKEAFHIAQKEHAKFTAIYIDVFE
                     KNRQYKDSQKQVHQHLMLAKSLGAKVKVVYSQTVALGLDEWCKNQDVTKLIIGQHIRN
                     KWRDFFNTPLIDHLMSFEHSYKVEIVPIKQIPVELKMNKSPYRPKGKRFAIDMLKMIL
                     IQIICVMMGLWIYQLDKHESSTIILMIFLIGIILLSIWTRSFIIGFLAAIINVFVFNY
                     FFTEPRYTFEVYRFDYPITFIVSILTSILTSALLKQIKFQYSITKKQLYRTDLLFQFN
                     DSIKQTYTVENLLINAGYQINQLLQQSITIYVINQSKVIKTIPLQNHIDNTTQQHEQA
                     LSWVIKNERQAGATTDTFPGINKWLIPIGTSPIKGILAIDYQSSQVINPYDASILESM
                     LNELSLAVENVTLLKQTRESMLQAERQLTHSNFLRSISHDIRTPLTTIMGNLDILVSH
                     SKDMSIIEKEQLLVHSFQESQYLYLLVTNILSLTKLQSSNVQIKLQPYLVSELVEEID
                     MILERRHLKKRITVSSSVDLQFIHIDSKLILQALFNLIENAVKHTSTDTKINLSIRYA
                     SDEQIEFAVIDEGPGISLEEQQKIFEPFYTGSNKYFKDNQKESMGLGLYLVQTILHKH
                     QSNLQYKPNQPHGSIFYFNIDTDFNEGDV"
     gene            7371..8066
                     /locus_tag="POB55_001326"
     CDS             7371..8066
                     /locus_tag="POB55_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDI6500295.1"
                     /translation="MQSKILIIEDDHAITHLLDVALTLDYYNVTTADNATQAHFKIQI
                     DKPDVILLDLGLPDKDGLCLISEIRQHTDIPIIVISARQEEQTIIQALDNGANDYMTK
                     PFNVDELRARIRVIERIAKSHQETNIVFTNGLLSIDFGSKSVVINNQEVHLTPNEFSL
                     LELLSNHKGKVLTYEMILKRIYGYVNKTEMPSLRVHMTSLRQKLSQCHEDAKDIIKTH
                     PRIGYQMLQWKEK"
     gene            complement(8435..9955)
                     /locus_tag="POB55_001327"
     CDS             complement(8435..9955)
                     /locus_tag="POB55_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDI6500296.1"
                     /translation="MQNFKELGISDNTVQSLESMGFKEPTPIQKDSIPYALQGIDILG
                     QAQTGTGKTGAFGIPLIEKVVGKQGVQSLILAPTRELAMQVAEQLREFSRGQGVQVVT
                     VFGGMPIERQIKALKKGPQIVVGTPGRVIDHLNRRTLKTDGIHTLILDEADEMMNMGF
                     IDDMRFIMDKIPAVQRQTMLFSATMPKAIQALVQQFMKSPKIIKTMNNEMSDPQIEEF
                     YTIVKELEKFDTFTNFLDVHQPELAIVFGRTKRRVDELTSALISKGYKAEGLHGDITQ
                     AKRLEVLKKFKNDQINILVATDVAARGLDISGVSHVYNFDIPQDTESYTHRIGRTGRA
                     GKEGIAVTFVNPIEMDYIRQIEDANGRKMSALRPPHRKEVLQAREDDIKEKVENWMSK
                     ESESRLKRISTELLNEYNDVDLVAALLQELVEANDEVEVQLTFEKPLSRKGRNGKPSG
                     SRNRNSKRGNPKFDSKSKRSKGYSSKKKSTKKFDRKEKSSGGSRPMKGRTFADHQK"
     gene            complement(10472..11830)
                     /locus_tag="POB55_001328"
     CDS             complement(10472..11830)
                     /locus_tag="POB55_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HDI6500297.1"
                     /translation="MINVTLKQIQSWIPCEIEDQFLNQVINGVTIDSRAISKNMLFIP
                     FKGENVDGHRFVSKALQDGAGAAFYQRGTPIDENVSGPIIWVEDTLTALQQLAQAYLR
                     HVNPKVIAVTGSNGKTTTKDMIESVLHTEFKVKKTQGNYNNEIGLPLTILELDNDTEI
                     SILEMGMSGFHEIEFLSNLAQPDIAVITNIGESHMQDLGSREGIAKAKSEITIGLKDN
                     GTFIYDGDEPLLKPHVKEVENAKCISIGVATDNALVCSVDDRDTTGISFTINNKEHYD
                     LPILGKHNMKNATIAIAVGHELGLTYNTIYQNLKNVSLTGMRMEQHTLENDITVINDA
                     YNASPTSMRAAIDTLSTLTGRRILILGDVLELGENSKEMHIGVGNYLEEKHIDVLYTF
                     GNEAKYIYDSGQQHVEKAQHFNSKDDMIEVLTSDLKAHDRVLVKGSRGMKLEEVVNAL
                     IS"
     gene            complement(11845..12915)
                     /locus_tag="POB55_001329"
     CDS             complement(11845..12915)
                     /locus_tag="POB55_001329"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDI6500298.1"
                     /translation="MTKENICIVFGGKSAEHEVSILTAQNVLNAIDKDKYHVDIIYIT
                     NDGDWRKQNNITAEIISTDELHLENGEALEISQLLKESSSGQPYDAVFPLLHGPNGED
                     GTIQGLFEVLDVPYVGNGVLSAASSMDKLVMKQLFEHRGLPQLPYISFLRSEYEKYEH
                     NILKLVNDKLNYPVFVKPANLGSSVGISKCNNEAELKEGIKEAFQFDRKLVIEQGVNA
                     REIEVAVLGNDYPEATWPGEVVKDVAFYDYKSKYKDGKVQLQIPADLDEDVQLTLRNM
                     ALEAFKATDCSGLVRADFFVTEDNQIYINETNAMPGFTAFSMYPKLWENMGLSYPELI
                     TKLIELAKERHQDKQKNKYKID"
     gene            13212..14414
                     /locus_tag="POB55_001330"
     CDS             13212..14414
                     /locus_tag="POB55_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HDI6500299.1"
                     /translation="MNYSSRQQPDKHWLRKVDWVLVATIAVLAIFSVLLINSAMGGGQ
                     YSANFGIRQIFYYILGAIFAGIIMFISPKKIKHYTYLLYFLICLLLIGLLVIPESPIT
                     PIINGAKSWYTFGPISIQPSEFMKIILILALARVVSRHNQFTFNKSFQSDLLLFFKII
                     GVSLVPSILILLQNDLGTTLVLAAIIAGVMLVSGITWRILAPIFITGIVGAMTVILGI
                     LYAPALIENLLGVQLYQMGRINSWLDPYTYSSGDGYHLTESLKAIGSGQLLGKGYNHG
                     EVYIPENHTDFIFSVIGEELGFIGSVILILIFLFLIFHLIRLAAKIEDQFNKIFIVGF
                     VTLLVFHILQNIGMTIQLLPITGIPLPFISYGGSALWSMMTGIGIVLSIYYHEPKRYV
                     DLYHPKSN"
BASE COUNT         4786 a         2638 c         2089 g         4932 t
ORIGIN      
        1 aattatagca tatggaatat ttcatgctaa tttattcttc ctaaaggtac atctaaaaat
       61 ttagttaagc agaaagtgct tgaatcgcta aaaagacacc atgttataat tttatcaaca
      121 tgatgccttt catctataat caatctttca tcttatcaag agcaatattt agttcaagca
      181 cattcacata atcatttgtt aacacaccac gctgcttacg atgttgaatc aagtcggcca
      241 ctcttgaagt agatatatga cgagcatcag caatacgagg tgcttgcttc aatgcatttt
      301 caaccgtaat atgcggatct aagcccgacc cagaacttgt tgcagcatct attgttacat
      361 ttgaattccc aaatttaaca tgatgtttca tgcgtgctat taattcggtg tttccattcg
      421 attcattact tccacctgat gatacgccgt ttttatataa tttttcagga ttcatattat
      481 aatcaactgc actcggtctc ccgtgaaaat atcgtgtctc tgtccagtgc tgtccaatca
      541 attttgatcc tactatacga ttgtcatacg taattaaact gccgtttgct tgttgataaa
      601 aaaatatttg accaattaac gtgatagcta acgggaataa aaatccacat aataccatag
      661 ttattatcgt taaacaaata ctatttctta tcgtattcat ggtacaggct ccttcctctt
      721 tacacaaaaa attgtacaat catatctatt aatttaatgc ctaaaaacgg gacgattaat
      781 ccacctaatc cataaatcaa catattattt ataaaaattc tatcaatgct gtaacccttt
      841 acttttacac ctttcatagc aattggaatt aaggcaacaa tgattaatgc attgaatatc
      901 aaagctgata taattgcaga tttaggtgac gataaatgca taatatttag tgaagtcatt
      961 tctggaattg ttgacatcat tagtgcaggt aaaattgcaa agtattttgc tacgtcatta
     1021 gccaaactaa atgtcgttaa tgcacctctc gtcattaata attgtttgcc tatttttaca
     1081 acctctatta actttgtagg attcgaatct aaatcaatta gattagctgc ctctttagca
     1141 ctaattgtcc ctgagttcat agctaatcct atattcgctt gtgctagcgc aggtgcatca
     1201 tttgtaccat ctcctgtcat cgcaacaata tggcctttcg cttgttcatc tttgatgact
     1261 ttaattttat cttcgggttt acactctgca acaaatctat caaccccggc ttcttttgca
     1321 attgtagctg ctgttaaagc attatcacct gtacacataa ctgtttcaat ccccattttt
     1381 ctcaattcag taaatcgttc tacaagacca tctttaatca catcttttaa ataaatcacg
     1441 ccaagcatga cattgttttc aatgactatt aatggtgtgc cacctttact cgatacatcc
     1501 atacagagag actcaatatt aagaggaata ttgccttgtt gttgtttgac aagatttatc
     1561 atactattag gtgcaccttt gaataccgat atttcatttg taatgattcc gctcattcta
     1621 gtttcagctg taaaagtctt atatgtgcca tcaatgtctt taggcagctc atttatatac
     1681 atctgcttcg ctaatcgtac aatacttttt ccttctggcg tatcatcgta gattgatgac
     1741 atataagcag cgactatcaa tttttccagc atttgttgat tcactggtaa aaattcacta
     1801 gcgattcgat tgccataagt gattgtgcct gtcttgtcta aaatcattac atcgacatct
     1861 ccacatactt ctacagcacg cccacttttc gctaatacat tgaattgagt aacacgatcc
     1921 atgcctgcaa taccaatcgc cgataacaaa ccaccgattg tcgttggtat taaacatact
     1981 gttaacgcaa tgagcatcgc aataggtaaa attaaatgca ggtaagatgc tattggatat
     2041 aacgttacaa taacgactaa aaatataatt gttaacgttg ttaataatgt aaaaagtgca
     2101 atttcatttg gtgttttatt tctttccgcc ccttcaacta aggcaatcat tttatctaaa
     2161 aaagatgtac ccgcttcact ctcaacacgt atttctaacc aatcagatgt tacaagtgta
     2221 ccgccaatga ctccatcaaa atcgccacct gattctttta tcacaggtgc agactcacca
     2281 gtaattgcag attcatcaac ggttgctaat ccatttatta caacgccatc agcagggatt
     2341 gtttctccat tttctacccg aatattttgt ccggctttta actctgtggc gttcactatc
     2401 cgatacgcac cattttcttc tatcaatcga gcagttaaat ttgattgtgc ttgtcttaaa
     2461 ctatcagctt gcgcttttcc acgaccttca gcaaaggctt ctgaaaaatt agcaaacaat
     2521 atagttatta ataatatgat aaaaattgta atcaaataac ctcgcgatag ataactagtt
     2581 ccaaatatgt caggaaaaca tattaatatc aacgttaaaa tcattccaac ctcaacgaca
     2641 aacattatcg gattttttat taattgttta ggattcagct tataaaaact cattttcaaa
     2701 gcttctatca ccattgtttg attgaaatat ttatttacat gatgcatttt ttccatccct
     2761 ttactttatt ttaaagttaa aaattcacca ataggaccaa gtaatagtac tggaataaat
     2821 gtcaaaccac ttagtaaaac gataaatacg attagtgata cgccaaaata aggtttatca
     2881 atcgctattg tatatttatc ttggtggtat gattttttat tcactaaact tgatgcaatc
     2941 attaattgca aaataattgg tatataacga gaaagcaaca taatgattcc tgtagagata
     3001 ttccagaatg ttgtatcatc tttcagtcct tcaaaccctg atccattgtt cgcagcagct
     3061 gatgtcattt catacataac ttgcgaaata ccatgaaaag acggattcgt tatactttca
     3121 cttgctccag gaatcataaa agcaagtgct gaaaatacta aaattaaaat tggatgtatg
     3181 agaaagacta agacaataca tttcatttca cgggcgccaa ttggcatatt taaatattct
     3241 ggtgttttac caaccatcaa actgcatata aacaccgtca gtaagacata tatcaataaa
     3301 ttcatgagtc ctacgccttc gccaccaaat acaacattta gcatcattaa taccattggt
     3361 cctaatccac ctaaaggcgt taagctatca tgcatgttat taacagaacc cgttgtaaat
     3421 gccgtcgtaa taactgtaaa tagtgctgac aagcctgctc caaaccgtac ctctttacct
     3481 tccatattcg gtccataaat gcctaaattc gctagtattg gattaccacg atactcactc
     3541 cacatagtta atgtaagaat tgctataaaa atgaaaaaca ttgcgacaaa taatatcaac
     3601 gcatgacgat gtactcgttt accatgtcta cttaacatgc gaccaaataa gaacaacatt
     3661 gacataggaa gtaacatcat actgcccatt tctataaaat tgctccaaat atttggattt
     3721 tcaaaaggtg ttgcagaatt tcctgctaaa aatcctccac cattcgtacc aagatgtttt
     3781 attgattcaa gtgatgcaat aggtccaaat gcaatatgtt gaatatgtcc gcttaaagtc
     3841 cgaatcatta aattagcatg caacgtttgt ggtacacctt gagtcatcaa taaaatacta
     3901 attaaacatg ataatggtaa aagtactcgg acaataaacc gaacaatatc ttgataaaaa
     3961 ttaccaatga tattagttaa tccagttaaa cgtctcaaca tcgctataca aacggcgtaa
     4021 cctgatgcac tagatgtaaa cattaaatat gtcattacaa tcatttgcgt taaatatgtc
     4081 acatctgatt caccgttata gtgttgtaaa ttactatttg ttaaaaaaga tattgctgta
     4141 ttaaacgcta aatctatcga ttggtttaaa ttatgatttg gatttaaaaa aagccattgc
     4201 tgaactatta gcaatacaaa tgttataaac cccataaatc cattaaatgc cagaaaatgt
     4261 ttgacatatg ttttagctga catgtgttct aaatctgtgc cgataatttt aaaacacata
     4321 ttttcaaatc tagtaaatat taaatctact cttgacgatt gcaccaatgc tacgcgatat
     4381 agatatccac taaaaacata cgtaatcata accatcattg ttagaaacaa aattatttcc
     4441 atgataaccc tcacttaata tatttctaaa atttttcact acgaattaag gcataaaata
     4501 aatacaaaac taatgcaata actaccagta ataaaacgat gagcattgtc ataacctcct
     4561 tacaacacaa caacatcgta acaacttgtt tatgagagaa atattaattt tcaaacttag
     4621 ttattaagaa atcattaaga tgtgtatgca gaaataaatt ttatagcatt taattgtgaa
     4681 gaatattatg atattgctat cgaggtgaag gttatgtcaa acactgaatc gctaaacata
     4741 ggaaaaaagc gtggatcttt aacaatttac atcggttaca gtccaggtgt tggtaaaaca
     4801 tttgagatgc tttcaaatgc cattgaacta tttcaaagta acgttgatat taaaatagga
     4861 tatattgaac cgcatcagcg tgatgaaaca aatgcattag ctgaacaatt acctaaaatc
     4921 acaaccaatt ttactaaaca tggtagtcat cattttcaat atttagatgt cgaccgcata
     4981 atcgaagaat cgccgacaat agtacttatc gatgagttag cacatacgaa tatttctaga
     5041 gatcgtcatg agaaacgata tatggatatt gaagaaattt taaatcatgg tatcgatgtt
     5101 cataccactt tgaacattca acatattgaa agtttaagta gtcaaattga actgatgacc
     5161 ggtatacatg ttaaagaacg tgtacccgac tatttcataa tgagcgccga tgtattagaa
     5221 gtcgtagata tctcacctga acaattaatt aaacgcttaa aagctggcaa ggtatataaa
     5281 aaggataggc tagatgtagc atttagtaat ttctttacgt atgcccacct aagcgaactg
     5341 cgtacattga cgttaagaac agttgccgac ttgatgagtg ataaagaaaa agtccgacac
     5401 aatcataaaa cgtcactcaa acctcatatt gctgtggcaa ttagtgggag catttataat
     5461 gaagcagtaa ttaaagaggc attccatatt gctcaaaaag aacatgcgaa attcactgct
     5521 atttatatag atgtattcga aaaaaacagg caatataaag atagtcaaaa gcaagtgcat
     5581 caacatctca tgcttgcaaa atcattagga gcaaaagtaa aagtagttta tagccaaacc
     5641 gttgcattag gattagacga atggtgtaaa aatcaagatg taaccaaatt aattatcgga
     5701 caacatatta gaaataagtg gcgagacttt ttcaatacac ctttaattga ccatttaatg
     5761 tcctttgaac atagctataa agtcgaaatc gttccaatca aacaaatacc tgttgaattg
     5821 aaaatgaaca aatcacccta tcgtcctaaa ggcaaacgtt tcgccataga tatgttaaaa
     5881 atgattttga ttcaaataat ttgtgtaatg atgggactgt ggatttatca acttgataag
     5941 catgagtcta gtacgattat tttaatgatt tttctcatcg gcatcatttt gttatccatt
     6001 tggacgcgat ccttcatcat tggcttttta gcagcaatta ttaacgtatt tgtttttaat
     6061 tattttttta cggaacctag atatacattt gaagtatatc gctttgacta tcctattaca
     6121 tttatcgtta gcattttaac aagtatttta acgagtgctt tattaaaaca aattaaattc
     6181 caatactcca ttactaaaaa gcaactttat cgtacagatt tattatttca atttaatgat
     6241 tcgattaaac aaacttatac agttgaaaac ttactaataa atgctggata ccaaattaat
     6301 caattattgc aacaatcaat tactatatac gttatcaatc aatcgaaagt aattaaaaca
     6361 ataccattgc aaaaccatat cgataatacg actcaacagc atgaacaagc attaagctgg
     6421 gtaattaaaa atgaaagaca agctggtgca acaacagata catttccagg cattaataaa
     6481 tggttgatac caattggcac atctccgatt aaaggcattc tagccattga ttaccaaagt
     6541 tctcaagtga ttaatccata tgatgcatca atattagaat caatgttaaa tgaactttct
     6601 ctcgccgttg aaaatgtgac gttacttaaa caaacgagag aatccatgtt acaggctgaa
     6661 cgtcaactga cacattcaaa ttttttaaga tcaatttcgc atgacatacg tacaccgtta
     6721 acaacaatta tgggaaattt agatatttta gtatcacata gtaaagatat gtctatcata
     6781 gaaaaggaac aattacttgt gcatagtttt caagaaagtc agtatttata tctattagtg
     6841 acaaatattt tatcattaac aaaattacag tcatcaaatg ttcagataaa attgcaacct
     6901 tatctcgtta gtgaattagt agaagaaatc gatatgattc tagaacgtcg ccatttaaaa
     6961 aagcgtataa ctgtatcatc ctcagtagat ttacagttta tacacattga cagtaagttg
     7021 attttacaag ctttattcaa tttgattgaa aatgcagtta aacacacttc tactgacacc
     7081 aaaatcaatt tatctattcg ttatgctagc gatgaacaaa tcgaatttgc cgttatagac
     7141 gaaggacctg gaattagttt agaagaacaa caaaagatat tcgagccttt ttacacagga
     7201 tcaaataaat attttaaaga taaccaaaaa gaaagcatgg gactaggctt atatttagta
     7261 cagactatcc tacacaaaca tcaatcaaac ttacaatata aaccgaacca accacacggc
     7321 agtatattct atttcaatat agatacagac tttaatgaag gagacgtata atgcaatcta
     7381 aaatattgat aattgaagat gatcacgcaa tcacacattt acttgatgtt gcattaactt
     7441 tagattatta caatgtaact acagccgaca atgccacaca agcacacttt aaaattcaaa
     7501 ttgataaacc agatgtcatt ttattagatt taggtttacc agataaagat ggattatgtt
     7561 tgatttcaga aatcaggcaa catactgaca ttcctatcat tgtaataagt gcaagacaag
     7621 aagaacaaac aattattcaa gctttagata acggtgcgaa tgactatatg actaaacctt
     7681 ttaatgttga tgagcttcgg gcacgaatac gagtcatcga gagaatcgct aagtcgcatc
     7741 aagaaactaa cattgtattt actaacggtt tgctaagcat cgactttggc tccaaatctg
     7801 ttgttattaa taatcaggag gtacatttga ctccaaatga atttagcttg ttagaactat
     7861 tgtcaaatca taaaggaaaa gtactcactt atgagatgat attaaaacga atatatggct
     7921 atgttaataa gactgaaatg cctagtttaa gagtgcatat gacatcatta agacaaaaac
     7981 tttcacaatg tcatgaggac gcaaaagata ttattaaaac acatccacgc attggttatc
     8041 aaatgttgca gtggaaagag aaataattaa ataaaaaaga tcctgccatt aaacgaatga
     8101 ttggcagtga tcttttttat attcaactta ttatttatcg cgcactagcg tcagcaaaaa
     8161 gccctattat ttttttacaa gaacatgata ctgaaatttc ataaagttta tttatgttcg
     8221 attattctta atttaaaaat agttctttaa attatatact caccacattt ggtggagaac
     8281 catatttaca cagaatgata taaccgcata cagatctcaa tccaattaaa tatttaacct
     8341 ttaaaacgtt agtactttga tatctacaga gaatcaatta ttgaaaacaa aaaagagctc
     8401 aagacattac atctttaagc tcttaatcta taaattattt ttgatggtca gcaaatgtgc
     8461 gacctttcat aggtctgctt ccaccgctgc tcttctcttt acggtcgaat ttttttgtac
     8521 ttttcttttt acttgagtat ccttttgaac gtttactctt actgtcaaat ttaggattac
     8581 cacgcttact atttctgtta cgagaaccgc ttggtttacc gttacggcct ttgcgagata
     8641 atggtttttc aaaagttaat tgaacttcaa cttcatcgtt cgcttctact aactcttgta
     8701 aaagtgcagc aactaaatca acatcgttat attcatttaa caactctgta gaaatgcgtt
     8761 tcaagcgtga ttctgactct ttagacatcc agttttcaac tttttctttg atgtcatctt
     8821 cacgtgcttg aagtacttct ttacgatgag gtggacgaag tgcgctcatt tttctaccat
     8881 tagcatcttc aatttgtcta atgtaatcca tttcaattgg atttacaaat gttaccgcga
     8941 taccttcttt accagcacga cccgtacgac caatacggtg tgtatagctt tcagtatctt
     9001 gaggtatatc aaagttataa acatgactca caccagaaat atctagtcct cttgctgcta
     9061 catcagtagc gactaaaata ttaatttggt catttttaaa tttctttaat acttctaaac
     9121 gtttcgcttg tgtaatatca ccatgtaagc cttcagcttt atatccttta gaaatcaaag
     9181 cacttgttaa ttcatcaaca cgacgttttg tacgtccgaa tacgattgct aattcaggtt
     9241 gatgaacatc taggaaattt gtaaatgtat caaatttctc taattcttta acaattgtat
     9301 agaattcttc gatttgtgga tcagacattt cattattcat tgtcttaatg atttttggtg
     9361 atttcataaa ttgttgtact aaagcttgga ttgctttagg cattgtagct gagaacaaca
     9421 ttgtttgacg ttgtactgct ggaattttat ccataataaa tctcatatca tcgatgaatc
     9481 ccatattcat catttcatca gcttcatcta aaatcaaagt atgaattccg tccgttttta
     9541 atgtgcgacg atttaaatgg tcgataacac gcccaggtgt tccgactacg atttgtgggc
     9601 cttttttcaa ggctttaatt tggcgttcga taggcatacc accgaataca gtaacaactt
     9661 ggacaccttg tccacggcta aattctctta attgttcagc tacctgcatt gccaattctc
     9721 ttgtaggtgc taaaatcaac gattgaaccc cttgtttccc tactactttc tcaattaaag
     9781 gaataccgaa tgctcctgtt ttacctgtac cggtttgagc ttgcccaagg atatcaattc
     9841 cttgtaacgc ataagggata ctgtcttttt ggataggtgt cggctcttta aatcccattg
     9901 attcaagtga ctgaaccgta ttatccgaaa tccctagttc tttaaaattt tgcaaaataa
     9961 ttctcctttt tacctgttta tttctcaata tacttttgca tataagttca ttaatatttc
    10021 atcaacttct atatagtacc accaaaaaaa tttaatcgca ataaagcaat ttgcgaatat
    10081 ttattagaat cacattaatt gattgtcata tttttgtcaa aaagctagca ttagataaga
    10141 tgtaagcgtt acgcatcatt cgaataatca ttataaaaaa ttttttataa aatacgtata
    10201 tcgttaacat tccacaacaa attttactga aacacatgtg aaattagaca ttataatact
    10261 acatcttttt gtaaaacatt atataaacat tcaaactact tcctatataa agtgtgtaca
    10321 tagaattaac cttgatttag tttcaaattt atagatttaa cataaaaaga aaaaggcaat
    10381 tttgtattta cacacaaaac cacctcttct ttgtaatctt ttagtattaa ttcaaatcat
    10441 ttttataagt aaaaggtccc tcgactaatc tctatgaaat taaagcattt accacttctt
    10501 ctaatttcat accacgtgat cctttaacta atacacggtc atgcgctttt aaatcgcttg
    10561 ttaaaacttc tatcatatcg tctttagaat tgaagtgttg tgctttttcg acatgttgct
    10621 ggcccgaatc ataaatatac ttcgcttcat taccaaacgt atacaacaca tctatatgct
    10681 tttcttctaa ataattacct acaccgatat gcatttcttt gctattttca cctaattcta
    10741 aaacatctcc taaaattaga atgcgacgcc ctgtcaaagt actcagtgta tcaatagctg
    10801 ctctcatact tgtaggactt gcattatagg catcatttat cacagtaata tcattttcta
    10861 atgtatgttg ctccatacgc ataccagtta agctgacatt ttttaaattt tgatagattg
    10921 tgttatatgt caaacctaat tcatgaccaa ccgcaatggc aatcgtcgca tttttcatat
    10981 tatgctttcc taatattggt agatcataat gttctttatt attaatcgta aatgaaatac
    11041 ctgtagtatc tctatcatca acagaacaaa ctaatgcatt atcagtagca acaccgatac
    11101 taatacattt tgcattttca acttctttaa catgcggttt caataatggc tcatcgccat
    11161 catatataaa cgtaccatta tcttttaaac ctattgtaat ttcagattta gctttagcaa
    11221 tcccctcgcg cgaacctaaa tcttgcatat gtgactcacc aatattagtt ataactgcaa
    11281 tatctggttg agcgaggttt gacagaaatt caatttcatg gaaacctgac atccccatct
    11341 ccaatattga tatttcagta tcattatcta attccaaaat agttaaaggt aaaccaattt
    11401 cattattgta attaccttgc gttttcttaa ctttaaattc ggtatgcaat acactttcaa
    11461 tcatatcttt agtcgttgtt ttaccattag accctgtgac ggcaattact ttagggttta
    11521 catgtctcaa gtaagcttgt gccaattgtt gtaatgccgt taatgtgtct tcaacccata
    11581 taataggccc gcttacattt tcatctatag gtgtccctct ttgataaaaa gcagctccag
    11641 caccatcttg taatgcttta gagacaaagc gatgaccgtc aacattttca cctttaaatg
    11701 gtataaataa catattttta gaaattgctc gtgaatcaat tgtgactcca tttatcactt
    11761 gatttaaaaa ttgatcttca atttcacaag gaatccatga ttgaatttgc tttaatgtaa
    11821 cattaatcat aataacaacc tcagttagtc aattttgtat ttatttttct gtttatcctg
    11881 gtgacgttct ttagcaagct cgataagttt tgtaatcagt tctggataag ataagcccat
    11941 attttcccat aactttggat acatactgaa agccgtaaat ccaggcattg catttgtttc
    12001 attaatatat atttggttgt cttctgttac aaagaaatct gcacggacta aaccagaaca
    12061 atctgtcgct ttgaatgcct ctaatgccat atttctaagc gttaattgaa catcttcgtc
    12121 taagtcagct ggaatttgta attgaacctt accatcttta tattttgatt tgtaatcgta
    12181 aaacgcgaca tcttttacga cttcacctgg ccatgtcgct tcaggatagt catttcctaa
    12241 aactgctact tcaatttcac gtgcgttaac gccttgttct ataacaagct tacggtcaaa
    12301 ttggaatgct tctttaatac cttctttaag ttccgcttca ttattacatt tactgatacc
    12361 tacacttgac cctaagttag caggtttaac aaagactggg taatttaatt tatcatttac
    12421 taattttaaa atgttatgtt catatttttc atattcagaa cgtaagaaac taatataagg
    12481 taactgtggt aaccctcgat gttcaaataa ttgtttcatt acaagtttgt ccatagaact
    12541 tgcagctgac aatacaccat ttcctacata tggtacatcc aaaacttcaa aaagtccttg
    12601 aatcgttcca tcttcaccat taggaccatg taataatggg aatactgcat cgtatggttg
    12661 tcctgaacta ctttctttca atagctgtga aatctcaagc gcctctccat tttctaaatg
    12721 aagctcatca gtagatataa tttcagctgt aatattattt tgctttctcc aatcaccatc
    12781 attggtaata taaatgatat caacatgata tttgtcttta tctattgcat ttaatacatt
    12841 ttgtgctgtc agaatcgata cttcgtgttc tgcacttttc cctccaaaaa cgatacaaat
    12901 attttctttt gtcatttcgt tttcctccaa tgatatatca gggtttgcta ccttaatata
    12961 aattaatatt gatttattcg catctttgaa tcttaatagt atcacatttt aatgctattt
    13021 tgcattttaa aaacgaggcg ttatgtgtct gtatctatgt tgattcattt ataatcagaa
    13081 caatattccc ctctatttta ttcctaattc aaattgaaat ctatagctga tatcactgtg
    13141 atattctagc taatttttaa aaagtcatgt aaaattgata taaacattag tgagtataaa
    13201 aggagtttgc aatgaattat tcatctcgtc aacagccgga taagcattgg cttcgcaaag
    13261 tagactgggt attagtagcc actatagctg ttttagcaat tttcagtgtt ctgcttatta
    13321 actcggcaat gggcggtggt caatacagtg ctaatttcgg tatcagacaa attttttatt
    13381 acattttagg tgcaattttt gcaggtatca tcatgtttat ttcacctaaa aagattaaac
    13441 attatacata tttattgtat ttcttaatct gtctattatt aataggcttg ctcgttattc
    13501 ctgagtcacc tattacacct attatcaatg gtgccaaaag ttggtacacg tttggcccta
    13561 tcagtattca gccatctgaa ttcatgaaaa ttattttaat tttagcatta gcgcgtgtcg
    13621 tttctagaca taatcaattc acattcaata aatcattcca aagtgatttg ttattatttt
    13681 tcaaaattat tggtgtctcg ttagtaccaa gtattttaat attactgcaa aatgacctag
    13741 gaactacatt agtattagct gcaattattg caggtgtgat gttagtaagt ggtataacat
    13801 ggcgtatctt agcacctatc tttattacag gtattgttgg tgcaatgaca gtcattttag
    13861 gtattctata tgcacccgca ttaattgaaa atttattagg tgtccaactg tatcaaatgg
    13921 gacgaatcaa ttcatggctt gacccctata catatagtag tggcgatggc tatcatttaa
    13981 ctgaatcact taaagctatc ggttctggac agttactagg taaaggatac aatcacggtg
    14041 aagtttatat acctgaaaat catactgact ttatcttttc agtgattgga gaggaactag
    14101 gttttatcgg ttctgtcata ttgatcttaa tatttttatt tttaatcttc catctaataa
    14161 gattagctgc gaaaattgaa gatcaattta acaaaatctt tatcgttggt ttcgttactt
    14221 tacttgtgtt ccatatttta caaaatattg gtatgacaat tcagttgtta ccaatcactg
    14281 gtattccatt accatttatt agttatggtg gtagtgcgct atggagtatg atgactggaa
    14341 taggtatagt cttatcaatt tattatcatg aaccaaaacg atatgtcgat ttataccatc
    14401 caaaaagtaa ttaatttaaa ctattttgag tttcaaatat cataa
//
LOCUS       DAMZKC010000061        14442 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.061, whole genome shotgun sequence.
ACCESSION   DAMZKC010000061 DAMZKC010000000
VERSION     DAMZKC010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14442
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.061"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..832
                     /gene="mreC"
                     /locus_tag="POB55_001331"
     CDS             <1..832
                     /gene="mreC"
                     /locus_tag="POB55_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HDI6500300.1"
                     /translation="FKNNKLIVVLCAIIVFIALIGLSIRSQSQSPPEQYIGDSVSFGQ
                     RVVSYPVNFVAGTIGDFFKKGDSKESKNKISQLESKNQQLEAENEKLKKELDLKDISK
                     FDPISTTVLARNPDQWMNTIVIDKGSKSGITSNMAVMTSQGFVGRVTKVNKFSSQVDL
                     ISTNTRAGKLSVNIQHGSKNIFGLIDRYDEKNSELVISDINNRDNISKGDKVVTSGLA
                     DQLPSNLYIGEVTKVQNDQYGLAKEVRVKTGADLTDLSHVYVAKRDPKTIPDDESRDK
                     "
     gene            832..1362
                     /gene="mreD"
                     /locus_tag="POB55_001332"
     CDS             832..1362
                     /gene="mreD"
                     /locus_tag="POB55_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HDI6500301.1"
                     /translation="MRTLYYFLIGILLFYIDTAIGLLIPMHIGKFELVFVPHLTFMYI
                     LMMVVYRGFGVSLLLSIFLGVMTDVYFGSIYGVYLFGYILFLALIDRFFKIFYKDHSM
                     LFIIILASTLLLEVYVALIYGMLGFIQFDIIHFVVFRLLPTLIMNFVLLIMLYPLIIK
                     FLKKTNNDIDMKRRQW"
     misc_feature    1389..1711
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            1724..2032
                     /gene="rplU"
                     /locus_tag="POB55_001333"
     CDS             1724..2032
                     /gene="rplU"
                     /locus_tag="POB55_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HDI6500302.1"
                     /translation="MFAIIETGGKQIKVEEGQEIFVEKLDVNEGDTFTFDKVLFVGGD
                     SVKVGAPTVEGATVTATVNKQGRGKKITVFTYKRRKNSKRKKGHRQPYTKLTIDKINA
                     "
     gene            2038..2358
                     /locus_tag="POB55_001334"
     CDS             2038..2358
                     /locus_tag="POB55_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HDI6500303.1"
                     /translation="MITVDITVNDEGKVTDVIMDGHADHGEYGHDIVCAGASAVLFGS
                     VNAIIGLTSERPDINYDDNGGHFHIRSVDTNNDEAQLILQTMLVSLQTIEEEYNENIR
                     LNYK"
     gene            2370..2654
                     /gene="rpmA"
                     /locus_tag="POB55_001335"
     CDS             2370..2654
                     /gene="rpmA"
                     /locus_tag="POB55_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009382703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HDI6500304.1"
                     /translation="MLKLNLQFFASKKGVSSTKNGRDSESKRLGAKRADGQFVTGGSI
                     LYRQRGTKIYPGENVGRGGDDTLFAKIDGVVKFERKGRDKKQVSVYAVAE"
     gene            3008..4300
                     /gene="obgE"
                     /locus_tag="POB55_001336"
     CDS             3008..4300
                     /gene="obgE"
                     /locus_tag="POB55_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020444729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HDI6500305.1"
                     /translation="MFVDQVKISLKAGDGGNGITAYRREKYVPFGGPAGGDGGKGASV
                     VFEVDEGLRTLLDFRYQRHFKASKGENGQSSNMHGKNAEDLVLKVPPGTIIKNVETDE
                     VLADLVEDGQRAVVAKGGRGGRGNSRFATPRNPAPDFSEKGEPGEELDVSLELKLLAD
                     VGLVGFPSVGKSTLLSIVSKAKPKIGAYHFTTIKPNLGVVSTPDQRSFVMADLPGLIE
                     GASDGVGLGHQFLRHVERTKVIVHMIDMSGSEGREPIEDYKVINQELAAYEQRLEDRP
                     QIVVANKMDLPESQDNLNLFKEEIGEDVPVIPVSTITRDNIDQLLYAIADKLEEYKDV
                     DFTVEEEESVGINRVLYKHTPSQDKFTISRDDDGAYVVSGNAIERMFKMTDFNSDPAV
                     RRFARQMRSMGIDDALRERGCKNGDIVRILGGEFEFVE"
     gene            4313..4768
                     /locus_tag="POB55_001337"
     CDS             4313..4768
                     /locus_tag="POB55_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HDI6500306.1"
                     /translation="MDNKDYKKFYLIREDVLPESVVKTLKIKDALKSDPTLSIYDAVK
                     QFDLSRSAFYKYRETIFPVDDKMLDHREFTLILYVTDIVGMLARVLDVISKLELSVLT
                     IHQSIPMEEKATITLSLNAKSKETSVEDVIGALRNLDYVSKVELISMSM"
     gene            4782..5384
                     /gene="ruvA"
                     /locus_tag="POB55_001338"
     CDS             4782..5384
                     /gene="ruvA"
                     /locus_tag="POB55_001338"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HDI6500307.1"
                     /translation="MYAYVKGKLTHLYPTHVVVETAGVGYEIQTPNSYRFQKHLDHEV
                     LIRTSLIVREDAQLLYGFSSEEEKDMFLSLIKVTGIGPKSALAILATSTPNEVKRAIE
                     NENDTYLTKFPGIGKKTARQIVLDLKGKVKITEEDSDSLLQVDATSTVQDQFVQEAML
                     ALEALGYSKRELAKVEKTLNKNKYDSVDEAVKAGLQLVVS"
     gene            5416..6420
                     /gene="ruvB"
                     /locus_tag="POB55_001339"
     CDS             5416..6420
                     /gene="ruvB"
                     /locus_tag="POB55_001339"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HDI6500308.1"
                     /translation="MNERMVDQSMHSEETDFELSLRPTRLRQYIGQNSIKSNLEVFIK
                     AAKLRHEPLDHVLLFGPPGLGKTTLSNIIANEMEVNIRTVSGPSLERPGDLAAILSGL
                     QPGDVLFIDEIHRLSSVVEEVLYPAMEDFFLDIIIGKGDEARSIRIDLPPFTLVGATT
                     RAGSLTGPLRDRFGVHLRLEYYNESDLKEIIIRTAEVLGTGIDEESAIELAKRSRGTP
                     RVANRLLKRVRDFQQVNEDEQIYIETTKHALGLLQVDQHGLDYIDHKMMNCIIKQYNG
                     GPVGLDTIAVTIGEERITIEDVYEPFLIQKGFLERTPRGRKATPLAYEHFAKSNEERG
                     "
     gene            6422..7447
                     /gene="queA"
                     /locus_tag="POB55_001340"
     CDS             6422..7447
                     /gene="queA"
                     /locus_tag="POB55_001340"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HDI6500309.1"
                     /translation="MNIEEFDYDLPESLIAQTPLKDRDHSRLLVMDRETGEMKHLHFK
                     DIIEYFRPGDTLVLNDTRVMPARLFGLKEETGAKVEMLMLTQIEGNDWEVLLKPAKRI
                     KVGNKLNFGNGKIIAECIKEMDQGGRIMRLHYEGILQERLDELGEMPLPPYIKERLDD
                     PDRYQTVYAKESGSAAAPTAGLHFTDELLTEIKNKGVNIAFVTLHVGLGTFRPVSVDD
                     VNDHEMHSEYYQMTQETADLLNDTKSKGHRIISVGTTSTRTLETIRRDHDKFVETSGW
                     TNIFIYPGFDFKAIDGQITNFHLPKSTLVMLVSAFSTRENVLNAYKTAVNLEYRFFSF
                     GDAMLII"
     gene            7470..8609
                     /gene="tgt"
                     /locus_tag="POB55_001341"
     CDS             7470..8609
                     /gene="tgt"
                     /locus_tag="POB55_001341"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016425271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HDI6500310.1"
                     /translation="MPAVTYEHIKTCKQSGARLGIVHTPHGSFETPMFMPVGTKATVK
                     TMSPEELRQIEAKIILGNTYHLWLQPGNDIIKHAGGLHKFMNWDGPILTDSGGFQVFS
                     LSNLRKITEEGVEFRHHTNGSKLFLSPEKSMQIQNDLGSDIMMAFDECPPMPAEYDYV
                     KKSIERTTRWAKRCLDAHQRPEDQALFGIIQGGEYEDLREQSAKDLVELDFPGYAIGG
                     LSVGEPKPVMYKMVEHTEQFMPKDKPRYLMGVGSPDALIECSIRGMDMFDCVLPTRIA
                     RNGTCMTSQGRLVIKNAKFADDLRPLDENCDCYTCQNYSRAYIRHLIKAEETFGIRLT
                     TIHNLHFLLKLMEDIRQAIREDRLLDFKEEFFEQYGLNVENPKNF"
     gene            8628..8888
                     /gene="yajC"
                     /locus_tag="POB55_001342"
     CDS             8628..8888
                     /gene="yajC"
                     /locus_tag="POB55_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDI6500311.1"
                     /translation="MQFSLLIYIVVIFAVMYFLMIRPQQKRAKQHRELINNIQSGQRI
                     TTIGGIKGTVKAVDETTVVITVNGHGTELTFEKPAIKQVDPS"
     gene            9163..11442
                     /gene="secDF"
                     /locus_tag="POB55_001343"
     CDS             9163..11442
                     /gene="secDF"
                     /locus_tag="POB55_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HDI6500312.1"
                     /translation="MKKSSRIIAFLLLVVLLFAGMAATYKSVIKNVNLGLDLQGGFEV
                     LYQVDPLNKGDKIDKKALQSTAQTLENRVNVLGVSEPKIQVEEPNRIRVQLAGVTDQN
                     EARKILSSQANLTIRDAEDKVKLSGSDIKQGSAKQEFKQETNQPTVTFKVKDKNKFKK
                     VTEEISKKRDNVMVVWLDFKKGDSYKKEAQKKNPKFISAASVDQPINSDSVEISGGFK
                     GQEGVKKAKQIAELLNAGSLPVDLKEIYSNSVGAQFGQDALDKTVFASFIGVALIYLF
                     MLGFYRLPGLVAIIALTTYIYLTLVAFNFISGVLTLPGLAALVLGVGMAVDANIIMYE
                     RIKDELRIGRTIKQAFSKANKSSFLTIFDSNLTTVIAAAVLFFFGESSVKGFATMLLL
                     GILMIFVTAVFLSRFLLSLLVSSNIFKNQFWLFGVKKNKRHDINEGVDVHDLKTSFEK
                     WNFVKLAKPLIGVSILIVVVGLVILYIFKLNLGIDFSSGTRVDFQSKQAITQQKVEQV
                     VKDSGLKADQIQINGKDNKVATVQFKDDLTRAQDNKLSDNIKSKFGDTPQINTVSPII
                     GQELAKNAMLALIYASIGIIIYVSLRFEWRMGLSSVLALLHDVFIIVAIFSLFRIEVD
                     LTFIAAVLTIVGYSINDTIVTFDRVRENLQKVKVITTTEQIDDIVNRSIRQTMTRSIN
                     TVLTVIVVVVAILFFGAPTIFNFTLALFIGLISGVFSSIFIAVPLWGIMKKRQLKKSP
                     KHKLVVYKEKKSNDEKILV"
     gene            11645..13918
                     /gene="recJ"
                     /locus_tag="POB55_001344"
     CDS             11645..13918
                     /gene="recJ"
                     /locus_tag="POB55_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDI6500313.1"
                     /translation="MIKPKYKWKLTKPAEYISDELTSKLKLTPIVKKILESKSIIDEQ
                     AIESIISDTDINHDALQLSDMTKTIERIKRAIANDEKILVYGDYDADGVTSTTILVTT
                     LQLLGAQVGWHIPNRFTEGYGPNELAFRNAHDEGITLIITVDNGIQGHNEIKMVQDLG
                     VDVIVTDHHEIGSTLPEAYAIVHPMHPSFNYPFQQLCGAGVAYKLAQALIENVPDYFK
                     AIVAIGTIADLVSLTDENRSLVKQGLKVLNDQCPTSVKALLKEAGYNDNIDEETIGFI
                     IGPRLNAVGRLDDASLACELLMTDDEEEAAFLAEQVEHFNRERKDIVATITEEAMAMA
                     ETKVKKGDLFLLLAKENWHEGVLGIVASKIVETFALPTLILNIDREQNHAKGSARSID
                     QVSMFEILSAHQELIAKFGGHHMAAGMTMDIENIESLAEGLNKWMKELSKTTSLDPVK
                     QVDVLLTENDITIKNIRDMNRLRPFGTDFSRPIFEMDDLSVSSVKAIGQQKNHLKLTL
                     GESNIAALFWQNGHLEPELQDEQPINILGSVQINEWNGNQSPQIIIQDIAMNEQQILD
                     YRSKRKSLPFTENDENIVVLIHPKSDKVNANEYYYGEEIKQQTDKVVLRDLPTSMEDL
                     SNSLQQLQFSQLYIVLQHNHSIYFDGIPNMDIFKKCYKALITKQETNIQKEGMLLCQH
                     LSVKPDTLKFMLKVFLDLKFVTQEDGLIRINQQPDKRSIDSSKVYQLRQQRMDVEKQL
                     LYQDFSEIKNWIKSQLS"
     gene            13940..>14442
                     /locus_tag="POB55_001345"
     CDS             13940..>14442
                     /locus_tag="POB55_001345"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009559512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HDI6500314.1"
                     /translation="MDLKQYVSEVQDWPKPGVSFKDITTIMDNGEAYGYATDKIVEYA
                     KDRDVDIVVGPEARGFIIGCPVAYSMGIGFAPVRKEGKLPREVIRYEYDLEYGTNVLT
                     MHKDAIKPGQRVLITDDLLATGGTIEAAIKLVEKLGGIVVGIAFIIELKYLNGIEKIK
                     DYDVMSL"
BASE COUNT         5156 a         1969 c         2820 g         4497 t
ORIGIN      
        1 ttttaaaaat aacaaattaa ttgttgtttt atgtgcaatt atcgttttta ttgcattaat
       61 tgggctgtcc atacgttcac aatctcaatc acctcctgaa caatatatag gtgattctgt
      121 gtcttttgga caacgagttg tgagttatcc agttaatttt gttgctggta cgattgggga
      181 cttttttaaa aaaggagatt ctaaagaatc taaaaataag attagccagt tagaatctaa
      241 gaaccaacaa ttagaagcgg aaaatgaaaa attaaaaaaa gagcttgatt taaaagatat
      301 ttcaaaattt gatcctattt ctactacggt tttagcaaga aatccggatc agtggatgaa
      361 tacaattgta attgataagg gatctaaatc aggtataact tcaaatatgg ctgtgatgac
      421 atcacaaggt tttgttggaa gagttactaa agttaataaa ttttcttcac aagttgattt
      481 aatctcaact aatacacgtg cgggtaaatt atctgtaaat atacaacacg gttctaaaaa
      541 tatatttggt ttaattgatc gttatgatga aaagaactca gaacttgtaa ttagtgacat
      601 taataataga gataatatct caaaaggtga taaagtcgtt acaagtggat tagctgatca
      661 actaccaagt aatttatata taggagaagt gactaaggtt caaaatgatc aatacggctt
      721 agctaaagaa gttagggtta agactggtgc agacttaaca gatttgagtc atgtttatgt
      781 tgcaaaaaga gatcctaaaa caattcctga tgatgaaagc agggataaat aatgcgtaca
      841 ctgtattatt ttttgatagg tattttacta ttttatatag atactgcaat tgggcttctt
      901 attccaatgc atataggtaa gtttgaactt gtatttgtac cacaccttac atttatgtat
      961 attttaatga tggtagtgta tcgtggcttt ggcgtatcat tattgctcag catattttta
     1021 ggcgtaatga ctgatgtata ctttggtagt atttatggag tgtacttatt tggctatata
     1081 ttgtttttag cacttataga tcgattcttt aaaatttttt acaaagatca ttcgatgtta
     1141 ttcattatta tattagccag caccttatta ttagaagtct atgtggcatt aatatacggt
     1201 atgttaggat tcattcaatt tgatattatt cattttgtag tctttagatt attgccaaca
     1261 ttaattatga attttgtact gttgattatg ctttatccgt tgattataaa gttccttaaa
     1321 aaaacaaaca atgacattga catgaaacgt cgtcaatggt aagatgcaat agttgagtag
     1381 ttataagtag ctacatacaa ccgctcaaat ataggttcaa gaacattttt aatgaagaaa
     1441 gcgattgtca atcaaggcgt tgaaggaaga aatgagaaga gtttatgttt ataaatgact
     1501 cgagttgcga ccgaaacaac gtagagtgca aacgcttgac tcatttgcag aagttattga
     1561 aagaaagcga ttgtcaatca aggcgttgaa ggaagaaacg agaagagttt atgtttataa
     1621 atgactcgag ttgcgaccga aacaacgtag agtgcaaacg cttgactcaa taacgaaatg
     1681 tcacctatgt atggcgtgac ttattataca ggaggtgcaa agtatgtttg ctattattga
     1741 aacaggtgga aaacaaatca aagtagaaga aggtcaagaa atcttcgttg aaaaattaga
     1801 cgtaaacgaa ggagatactt ttacatttga taaagtatta tttgtaggtg gagattcagt
     1861 taaagttgga gcgccaacag ttgaaggtgc aacagttact gctactgtta ataaacaagg
     1921 tcgcggtaaa aaaatcactg tattcacata caaacgtcgt aaaaattcaa aacgtaaaaa
     1981 aggccatcgt caaccataca ctaaattaac aatcgataaa atcaacgcgt aattattatg
     2041 attactgttg atattacagt taatgatgaa ggcaaagtaa cagacgttat tatggatggc
     2101 catgctgacc atggtgaata tggtcatgat atcgtttgtg ctggagcttc agctgtattg
     2161 tttggtagtg ttaatgcgat tataggattg acatctgaga gaccagatat caattatgac
     2221 gacaatggtg gtcattttca tataagaagc gttgatacaa ataacgatga agcgcaacta
     2281 attcttcaaa caatgcttgt gtctttacaa actattgaag aagaatataa tgagaatatt
     2341 agattaaatt ataagtgagg tgcattccga tgttaaaatt aaacttacaa ttcttcgcat
     2401 ctaaaaaagg ggtaagttct acaaaaaacg gacgtgactc tgaatcaaaa cgcttaggtg
     2461 ctaaacgtgc tgacggtcaa ttcgtaacag gtggttcaat tttatatcgc caacgtggta
     2521 ctaaaattta ccctggtgaa aatgtaggtc gtggtggcga tgatacatta ttcgctaaaa
     2581 tcgacggcgt tgttaaattc gaacgtaaag gtcgcgacaa aaaacaagtt tctgtatatg
     2641 cagtagctga ataattttgt ctagttaaca ccagaagtga atcttctggt gttttttact
     2701 ttttataaaa ggtttttcat tattatttta attatgttat taaaaaatgg gaattttaga
     2761 cgttatacta aatgtacaac tgcataagag cccctaatcg ctaaactata aggggacaaa
     2821 agaatacagt tgcagtataa aaagcaaaaa aagtatcata tttggtactc ggaaatatca
     2881 gcaatttgag taaagtaaat ttaaaagcaa ctgcataaga gcccctaatt atttacaata
     2941 tataaggggc tctaatatgc tataattatt gggaaaatga aaattatatg taaaagaggt
     3001 gagatatatg tttgtcgatc aagtcaaaat atctcttaaa gccggtgatg gtggtaatgg
     3061 tattaccgca tatagaagag aaaaatatgt accatttggt ggaccagctg gcggtgacgg
     3121 tggtaaaggt gcttcagtcg tatttgaagt ggatgaaggt ttaagaacgt tattagattt
     3181 tagatatcaa cgtcatttta aagcaagcaa aggtgaaaat ggccaaagta gtaatatgca
     3241 tggtaaaaat gcggaagatt tagtattaaa agttccacct ggtacaatta ttaaaaatgt
     3301 tgaaacagac gaagtgttag cagatcttgt tgaagatggt caaagagctg tagtagcgaa
     3361 gggcggtcga ggtggccgag gtaattcacg ttttgcaaca cctagaaacc ctgcacctga
     3421 cttcagtgaa aaaggtgaac caggtgagga attagatgta tctttagaat tgaaattatt
     3481 agctgatgta ggattagtag gtttccctag tgtgggtaaa tcgactttat tatctatcgt
     3541 ttcaaaagct aagcctaaaa ttggggcata tcattttaca acgattaaac caaatctagg
     3601 tgttgtttca acgcctgatc aacgtagttt tgttatggca gatttaccag gtttaattga
     3661 aggtgcatct gatggcgttg gattaggaca tcaattttta agacatgtag agagaacaaa
     3721 agttattgtt cacatgattg atatgagcgg ttctgaaggt agagaaccta ttgaagatta
     3781 taaagtcatt aatcaagaat tagctgcgta cgagcaacgt ttagaagata gacctcaaat
     3841 cgtagtagct aacaagatgg atttacctga atcacaagat aatttaaact tgtttaaaga
     3901 agaaattggc gaagatgtgc cagttattcc agtttcaaca ataacgcgtg ataatattga
     3961 tcaattatta tatgcaatag cagataaatt agaagaatat aaagatgttg acttcacagt
     4021 tgaagaagag gagtcagttg gcattaaccg agtattatat aaacatacac cgtcacaaga
     4081 taaatttaca atttcaagag atgatgatgg tgcttatgtg gtaagtggta atgctattga
     4141 aagaatgttt aaaatgactg actttaacag tgatccagca gtacgtcgat ttgctcgtca
     4201 aatgcgttcg atgggtattg atgatgcact tagagaacgt ggttgtaaaa atggtgatat
     4261 cgttagaatt cttggcggag aatttgaatt cgttgaatag gagcgtaaca tgatggacaa
     4321 taaagattat aaaaagtttt atttaattag agaagatgtc ttgcctgaat ccgtggttaa
     4381 aacattgaag attaaagatg ccttaaaaag tgatccgaca ttgtccattt atgatgccgt
     4441 taaacagttt gatctatcta gaagtgcttt ttataaatat agagaaacga tatttccagt
     4501 agacgataaa atgcttgacc atcgagaatt tacattaatt ttatatgtaa ctgatattgt
     4561 gggtatgttg gcacgtgtac tagatgttat atcaaagtta gaactatctg tattaacgat
     4621 tcatcaaagt attccaatgg aagaaaaagc aacaataaca ttatcactga atgctaaatc
     4681 taaagaaact tcagtagaag atgttattgg tgctttgaga aatttagatt atgtatcaaa
     4741 agtagaatta attagtatga gtatgtaagg atgtgcctat aatgtacgcg tatgtcaaag
     4801 gtaagttaac acatttatat cctacacacg tagttgttga aactgctggt gttggttatg
     4861 aaattcaaac accaaattct tatcgttttc aaaagcatct agatcatgaa gttttaattc
     4921 gtacatcttt aattgttcgt gaagatgcac aattattgta tggatttagt agtgaagaag
     4981 agaaagatat gttcttgagt ttaattaaag ttactggtat tggtccgaaa tcagctttag
     5041 ctattttagc gacaagtacg cctaatgaag taaaacgtgc cattgaaaat gaaaatgata
     5101 cgtatttaac taaattccca ggaattggta agaaaacggc aagacagatt gtcttagatt
     5161 taaaaggtaa agtgaaaatc actgaagaag atagcgattc attattacaa gtagacgcta
     5221 cttcgacggt gcaagatcaa ttcgtgcaag aagcaatgtt agcgttagaa gcattaggtt
     5281 attctaaacg agagcttgca aaagttgaga aaacgttaaa taaaaataaa tatgactcag
     5341 ttgatgaagc tgttaaggca ggtcttcaat tagttgtatc ttaattttaa atagattaat
     5401 aggggaagtg ttgtcatgaa tgagcgtatg gttgatcaat caatgcatag tgaagaaact
     5461 gatttcgaat tgtcgcttag acctacgaga ttacgacaat atattggtca aaattcaata
     5521 aaaagtaatt tagaagtatt tattaaagcg gctaaacttc gtcatgaacc attagatcat
     5581 gtattgcttt ttggcccccc tggattaggt aagacaacat tatctaatat cattgccaat
     5641 gaaatggaag ttaatatacg tacagtatca gggccttcat tagaaagacc tggtgatttg
     5701 gctgcaattt tatcaggact tcaacctgga gatgtattgt ttattgatga aatacacaga
     5761 ctgagtagtg ttgttgaaga agtgttatac cctgcaatgg aagatttctt tttagatatt
     5821 atcattggta aaggcgatga ggctagaagt atccgtatcg acttacctcc attcactttg
     5881 gtaggtgcaa caacgcgagc tggcagctta acaggtccac taagggatcg atttggtgtg
     5941 cacttaagat tagaatatta taatgaatca gatttaaaag aaatcattat tagaacagct
     6001 gaggttttag gcacaggtat tgatgaagaa agtgccattg aacttgctaa acgttctaga
     6061 gggactccaa gagtagcaaa tcgactattg aagcgggtaa gagacttcca gcaagtgaat
     6121 gaagatgaac aaatatacat tgaaacaacg aagcacgcat taggtttact tcaagttgat
     6181 caacacggac tagattacat tgatcataaa atgatgaact gtattattaa gcagtataat
     6241 ggtggacctg ttggtttaga tacgattgcc gtaacaattg gtgaagaacg tattacaatt
     6301 gaggacgttt atgagccatt tcttattcag aaaggctttt tagaacgtac gccacgtggc
     6361 agaaaagcaa caccattagc ttatgaacat tttgcaaagt cgaatgagga gagaggataa
     6421 cgtgaatatt gaagaatttg actatgactt accagaatca ttaattgctc aaacgccttt
     6481 aaaagatcgt gatcatagtc gtttattagt catggataga gaaactggtg aaatgaaaca
     6541 tttacatttc aaagatatca ttgagtattt tagacctggt gatacattag tgcttaacga
     6601 tacgcgagta atgccagcta gactttttgg tttaaaagaa gaaactggtg caaaagttga
     6661 aatgttaatg ttaactcaaa ttgaaggtaa tgattgggaa gtcttactga aaccagctaa
     6721 gcgtattaaa gttggtaata aattgaattt tggtaatggc aaaattatag ctgagtgcat
     6781 aaaagaaatg gatcaaggtg gacgcatcat gcgtttacat tatgaaggta ttttacaaga
     6841 aagattagat gaattagggg aaatgccact gccaccatac atcaaagaac gtttagatga
     6901 tccagatcgt tatcaaacag tttacgctaa agaaagtggt tcagcggcag caccaacagc
     6961 aggattacat tttactgatg agttattaac tgaaattaaa aataaaggcg ttaatatcgc
     7021 atttgttaca ttacatgttg ggttaggtac gtttagaccg gtgagcgtcg atgatgtgaa
     7081 tgaccacgaa atgcatagtg aatattatca aatgacacaa gaaacagctg atttattaaa
     7141 tgatactaag tccaaaggac atcgcattat atcagttggt acaacttcaa cacgtacact
     7201 tgaaacaatt cgacgcgatc atgataaatt tgttgaaacg agtggctgga ctaatatatt
     7261 tatttatcca ggatttgatt ttaaagcaat tgatggccag attactaatt ttcatttacc
     7321 aaaatcaaca ttagttatgc tagtatcagc gtttagtact cgtgaaaatg ttctgaatgc
     7381 ttataaaaca gcagtaaatt tagaatatag attctttagt tttggcgatg caatgttaat
     7441 tatataaaaa gaatgtgagg attttgaata tgcctgcagt aacatacgaa cacattaaaa
     7501 cttgtaaaca atcaggtgcg cgtttaggta tcgtgcacac accacacggt tcatttgaaa
     7561 cacctatgtt tatgccagtt ggtactaaag caaccgttaa aacaatgagt ccagaagagt
     7621 taagacaaat tgaagcaaaa atcattttgg gcaacacata tcatttgtgg ttacaacccg
     7681 gaaatgatat tatcaaacac gctgggggat tacataaatt catgaattgg gatggtccga
     7741 ttcttacaga ttcaggcggt ttccaagtgt ttagtttaag taatttacgt aaaattacag
     7801 aagaaggcgt ggaatttaga catcatacta atgggtctaa attatttttg agtcctgaga
     7861 aatcaatgca aattcaaaat gatttaggat ctgatattat gatggcattt gatgaatgtc
     7921 caccgatgcc tgctgaatat gattatgtaa aaaaatctat tgaacgtaca acacgttggg
     7981 cgaaaagatg tctagatgca caccaaagac ctgaagatca agcattgttc ggcattatac
     8041 aaggtggcga atatgaagat ttaagagaac aaagtgcaaa ggatttagta gaattagatt
     8101 ttcctggtta tgcaatcggc ggtttgtcag ttggtgaacc taaaccggtt atgtataaaa
     8161 tggttgaaca tacagagcag tttatgccta aagataaacc aagatattta atgggtgtag
     8221 gatctccaga tgcgttaatc gaatgtagta ttcgcggcat ggatatgttt gattgtgtct
     8281 taccgacacg tattgccaga aatggtactt gtatgacatc gcaaggtcgt ttagttatta
     8341 aaaatgcaaa atttgcagat gatttaagac cgttagatga gaattgtgac tgttatacat
     8401 gtcaaaacta ttcaagagcg tatatacgtc atttaatcaa ggcagaggaa acttttggta
     8461 ttcgtcttac tactattcat aatttacatt ttctgctaaa attaatggaa gatataagac
     8521 aagccattcg agaagatcgt cttttagatt tcaaagaaga attcttcgag caatatggat
     8581 taaatgttga gaacccaaaa aacttttaag caagaggagc gtataaaatg caattttcat
     8641 tactaatata tatagtcgta atttttgcgg ttatgtattt cttgatgatc agaccacaac
     8701 aaaaacgtgc gaaacagcat cgtgagttga ttaataacat tcaatctggt caaagaatta
     8761 caactattgg tggtattaaa ggtactgtta aagcagtaga tgaaacaact gttgttatta
     8821 cagttaatgg tcatggtact gaattaactt tcgaaaaacc tgctattaaa caagttgacc
     8881 cttcataatg agaagtggta ttaaaaagga tgaaacgaat agcgtttcat cctttttaag
     8941 ttataagtag tatgtagaat agcttgtttt tattccaaaa aaagaaattg tgatacataa
     9001 taattgttaa atttaaatta aatatatcta tgactatttt tactccctgc tctagtatgt
     9061 taatatgtat aaggctaata aattttgtaa gaaagattta acttgcgtta taaaatagta
     9121 ttcaagtatt ctaaatacat acgtaaataa tgaggtgttc atgtgaagaa aagtagtaga
     9181 ataattgcgt tcttgctttt agtcgtattg ttgtttgccg gaatggctgc aacttataaa
     9241 agtgttataa agaatgtcaa tttaggactt gatttgcaag gtggatttga ggtgctttat
     9301 caagtcgatc ctttaaataa aggcgacaaa atagataaaa aagccttaca atcaacagcg
     9361 caaacattag aaaatcgtgt taacgtttta ggtgtttcgg aacctaaaat tcaagttgaa
     9421 gaacctaata gaattagagt acaacttgct ggtgtaacag accagaatga agcacgtaaa
     9481 attttatctt ctcaagctaa tttaacaatt cgcgatgcag aagataaagt aaaattaagc
     9541 ggttctgata ttaaacaggg atctgccaaa caagaattta aacaggaaac aaaccagcct
     9601 acagttactt ttaaagtaaa agacaaaaac aaatttaaaa aagtaacgga agaaatctct
     9661 aagaaacgtg ataatgtcat ggttgtttgg ttagatttca aaaaaggtga cagctataaa
     9721 aaagaagcac aaaagaaaaa ccctaaattt atttctgcag caagtgttga tcaaccaatc
     9781 aattctgata gcgtagaaat ctcgggtgga tttaaaggac aagaaggcgt taaaaaagca
     9841 aaacaaatcg ccgaattgtt aaatgcaggt tctttaccag ttgacttgaa agaaatttat
     9901 tcaaactcag taggtgccca atttggtcaa gatgcattag ataaaactgt gtttgcatcg
     9961 tttattggtg tagcattaat ttatttattc atgcttggat tctaccgatt acctggttta
    10021 gtagcgatta ttgcattgac aacttatatc tatctaacgt tagtagcatt taatttcatt
    10081 tccggggttc taactttacc aggattagcg gcgttggtat taggtgtagg tatggctgta
    10141 gatgccaata ttatcatgta tgagcgtatt aaggatgaac ttcgaatagg tagaacgata
    10201 aagcaagcct tttctaaagc aaacaaaagt tcattcctaa caatttttga ttctaactta
    10261 acaacagtta tcgccgcagc agtattattc ttcttcggtg aaagttcagt taaaggtttc
    10321 gcgacaatgt tattattagg tattctaatg atctttgtta cagccgtgtt cttatcaaga
    10381 ttcttattat cattacttgt ttcatcaaat atattcaaaa atcaattttg gttatttggt
    10441 gttaaaaaga ataaacgaca tgatattaat gaaggtgtag atgttcatga ccttaaaact
    10501 tcattcgaga aatggaattt tgttaaatta gctaagccat taattggagt aagtatttta
    10561 attgtagttg tcggtttagt tattctttat atcttcaagt taaacttagg aattgatttc
    10621 tcaagtggta cacgcgtaga tttccaatct aaacaagcaa ttactcaaca aaaggtagag
    10681 caagttgtaa aagatagtgg acttaaagcg gatcaaattc agatcaatgg taaagacaat
    10741 aaagtagcaa ctgttcaatt taaagacgat ttaacgcgtg ctcaagataa taaattgagt
    10801 gataatatta aatcgaaatt cggtgacaca cctcaaatta atactgtatc accaattatt
    10861 ggacaggaat tagcaaaaaa tgcaatgctt gcattaatct atgcatcgat aggtatcatc
    10921 atctatgtat cattacgatt tgaatggcga atgggtcttt catctgtatt ggcattatta
    10981 catgatgtat tcattatagt agcgattttc agtttattta gaattgaagt agatttaaca
    11041 tttatcgccg ctgtattaac aattgtcggt tattcaatta atgatacaat cgtaacgttt
    11101 gaccgtgtac gtgaaaactt acaaaaggtt aaagtgatta cgacaacaga acaaattgat
    11161 gatatcgtta atagatcaat tagacagaca atgacacgtt caattaatac agtattaaca
    11221 gttattgtag tagtagttgc tatactattc ttcggtgctc ctacgatatt caactttact
    11281 ttagcattat ttatcggatt gatttctggt gtattctctt caatcttcat tgccgttccg
    11341 ctatggggaa taatgaaaaa acgtcagctg aaaaaatcgc cgaaacacaa attagttgta
    11401 tataaagaaa agaaatcgaa cgatgaaaag attttagttt aaaatgaatt aagcggtatg
    11461 tgaaacaata aagagttcac ataccgcttt ttactttatt taaatttaac aaaatatata
    11521 taaataagtt cccgcattca tttttaaaaa atggtataat taaagtcttg attatttaga
    11581 taagtgcttt ttatttaaag taattttaga tataatgtct tttggaaatg aggcgtaaat
    11641 atatatgatt aaaccgaagt ataagtggaa attaactaaa ccagctgaat acataagtga
    11701 tgaattaaca tcaaaattaa agttaacgcc aatcgtaaaa aaaatattag aaagtaaatc
    11761 gattattgat gaacaagcaa tagaatccat catttcagat acagatatca atcatgatgc
    11821 attgcaattg agcgatatga ctaagactat tgaaagaatt aaaagagcga tagcgaatga
    11881 tgagaaaatc ctagtttatg gtgattacga tgctgacggt gttacctcaa ctacaatttt
    11941 agtgacaaca ttacaacttt taggtgcaca agtaggatgg catattccta accgctttac
    12001 cgaaggatat ggaccaaacg aactagcatt tagaaatgct catgatgaag gcataacatt
    12061 aatcataact gttgataatg gcatacaagg tcataatgaa attaaaatgg ttcaagattt
    12121 aggagttgat gtcattgtaa ctgatcatca tgaaattggt agtacattac cagaagcata
    12181 tgcaatcgta catccaatgc atccatcgtt taattatccg ttccaacaat tgtgtggtgc
    12241 aggtgttgca tacaaattag cacaagcatt gattgaaaat gtacctgatt attttaaagc
    12301 aatagttgcg ataggtacca ttgcggattt agtatcctta actgatgaaa atcgatcatt
    12361 agtgaaacaa ggattaaaag tcttaaatga tcaatgtccg acctctgtta aagcattgct
    12421 aaaagaagcg ggatacaatg acaatattga tgaagaaaca attggtttta ttattggacc
    12481 tagacttaat gctgtaggtc gtttagatga cgcatcatta gcttgcgaat tgttgatgac
    12541 tgacgacgaa gaagaagctg catttttagc agaacaagtt gaacatttta accgtgaaag
    12601 aaaagatatt gtagcaacta tcacagaaga agcaatggct atggcagaaa cgaaagttaa
    12661 aaagggagat ttatttttac ttttagcaaa agaaaattgg catgaaggtg tcttaggtat
    12721 tgttgcttcg aaaattgtcg aaacatttgc tttgccaaca ttaattttaa atattgatag
    12781 ggagcaaaat catgcaaaag gttccgccag atcaattgat caagtgtcga tgtttgaaat
    12841 actatcagca catcaagagt taatcgctaa atttggcggc catcatatgg ctgcaggtat
    12901 gacgatggat attgaaaata tagaatcgtt agctgaaggt ttaaataaat ggatgaaaga
    12961 attatctaaa acaacttcgt tagatcccgt aaagcaagta gatgtattat taacagaaaa
    13021 tgatataacg attaaaaata ttcgtgatat gaatcgttta agacccttcg gaacagattt
    13081 ttctagacct atttttgaaa tggatgattt atcagtttct tcagttaagg cgataggtca
    13141 acaaaaaaat catctaaaat tgacacttgg tgaaagtaat atcgccgcgc tattttggca
    13201 aaatggacac ttagaacctg agttacaaga tgaacagcca attaatatat taggatctgt
    13261 tcaaatcaac gaatggaatg gtaatcaatc accgcaaata attattcaag atattgcgat
    13321 gaatgaacag caaatattag attatagaag taagcgaaaa agtttacctt ttacagaaaa
    13381 tgatgaaaat attgtcgtgc ttattcatcc taaaagtgat aaagtaaatg cgaatgaata
    13441 ttattatggt gaagaaatta aacaacaaac tgataaagta gtattaagag atttaccaac
    13501 gtcaatggaa gacttgtcta attccttgca acaactgcaa ttttctcaac tttatatagt
    13561 tttgcaacat aatcattcga tttacttcga tggtatacct aatatggata tttttaaaaa
    13621 gtgttataaa gcattaataa ctaaacaaga aacaaatatc cagaaagagg gtatgttatt
    13681 gtgtcaacat ttaagtgtga aaccagatac acttaaattc atgttgaaag ttttcttaga
    13741 cttaaaattt gtaacacaag aagatggttt aattcgaatc aatcaacaac ctgataaaag
    13801 atcgattgat tccagcaaag tatatcaatt aagacaacaa cgtatggatg ttgaaaagca
    13861 attattatat caagattttt cagaaataaa aaattggata aagtcacaat tgtcgtgagc
    13921 aatttaggag gaaatattaa tggatttaaa gcaatacgta tcagaagttc aagattggcc
    13981 gaaaccaggt gttagtttca aggatattac tacaattatg gataatggtg aagcatatgg
    14041 ctatgcaaca gataaaattg tagaatacgc aaaagacaga gatgttgata tcgttgtagg
    14101 acctgaagcg cgtggcttta tcattggctg tcctgtagct tattcaatgg ggattggctt
    14161 tgcacctgtt agaaaagaag ggaaattacc tcgtgaagtc attcgttatg agtatgacct
    14221 agaatatggt acaaatgttt taacaatgca caaagatgca attaaaccag gtcaacgtgt
    14281 gttaattaca gatgatttat tagctactgg tggtacgatt gaagcagcaa taaaattagt
    14341 tgaaaaatta ggcggtatcg tagtaggtat tgcatttata attgaattga aatatttaaa
    14401 tggtattgaa aaaattaaag attacgatgt tatgagttta at
//
LOCUS       DAMZKC010000062        14408 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.062, whole genome shotgun sequence.
ACCESSION   DAMZKC010000062 DAMZKC010000000
VERSION     DAMZKC010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14408
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.062"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            57..977
                     /gene="rnz"
                     /locus_tag="POB55_001346"
     CDS             57..977
                     /gene="rnz"
                     /locus_tag="POB55_001346"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HDI6500315.1"
                     /translation="MEVTFFGTSAGLPTKERNTQAIALNLEPYSNSIWLFDVGEGTQH
                     QILHHAIKLGKVTHIFITHMHGDHIFGLPGLLSSRSFQGGEQKPLTLVGPKGIKAYVE
                     MSMNLSESHLNYPITYIEIDDHLTYHHDGFTVEAHLLNHGVPSYGYRVMAPETTGTIN
                     VEALKNIGLEPGPKYQEVKSHDTFEHNGQVYQSKDFRGESKQGPVVAIFGDTKPCSNE
                     RVISRDADVMVHEATYIDGEKHLANNYHHSHIEDVFALIKEANVKRTLITHLSNRYNT
                     EDINEIYQTLIQNEDTPNFNFVKDFDSFKV"
     gene            complement(1080..1895)
                     /gene="proC"
                     /locus_tag="POB55_001347"
     CDS             complement(1080..1895)
                     /gene="proC"
                     /locus_tag="POB55_001347"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDI6500316.1"
                     /translation="MKLVFYGAGNMAQAIFTGIINSSNLDANDIYLTNKSNEQALKAF
                     AEKLGVNYSYDDATLLKDADYVFLGTKPHDFDALATRIKPHITKDNCFISIMAGIPID
                     YIKQQLECQNPVARIMPNTNAQVGHSVTGISFSNNFDPKSKDEINDLVKAFGSVIEVS
                     EDHLHQVTAITGSGPAFLYHVFEQYVKAGTKLGLEKEQVEESIRNLIIGTSKMIERSD
                     LSMAQLRKNITSKGGTTQAGLDTLSQYDLVSIFEDCLNAAVDRSIELSNVEDQ"
     gene            2037..2792
                     /locus_tag="POB55_001348"
     CDS             2037..2792
                     /locus_tag="POB55_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDI6500317.1"
                     /translation="MKGQHFIVTGGTSGLGLSIVRKLLENKVHVTLLVRDVDKATRIF
                     EQELGKTINVIPCDLNDLKSIQALQFEDNTSFDGFIYSAGLGYFKSISDHSFSEMIET
                     YQLNLISFNVLYTVLRPYLTSNAHIVGISSQAAFSTQANAAHYGASKAGFYALMNALR
                     LESPNLHIMTVNVGPIDTPFHQKADPSMKYAKRMGKIMLDANQLAEDIIYGIKTKQLE
                     INRPKWMHHALKMYQIAPRFFERCFPKLFKNKA"
     gene            2808..3056
                     /locus_tag="POB55_001349"
     CDS             2808..3056
                     /locus_tag="POB55_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500318.1"
                     /translation="MNCQSESVVRFCVRYAEQLSVFEEFTVLDILGDISVDQISDSTL
                     YYTCEKFKLLVLQGNVLGVQIITNNDESTCEVKYRKMF"
     gene            complement(3272..4180)
                     /locus_tag="POB55_001350"
     CDS             complement(3272..4180)
                     /locus_tag="POB55_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDI6500319.1"
                     /translation="MQKNILKSGISLSELGLGCMSLGTDLKKAEQIIDCAVENGITYF
                     DTADMYDKGINESVVGKALLKYQQRDDIVIGTKVGNRLTKDGSTKWDPSKSYIKEAVK
                     GSLKRLGIDHIDLYQLHGGTIDDPLDETISAFDELKQEGIIRAYGISSIRPNVIDYYL
                     KHSQIETIMSQFNLIDNRPESLLDAIHNNDVKVLARGPVSKGLLTSNSVNVLDNKFKD
                     GIFDYSHDELGETIASIKEIESNLSALTFSYLTSHDVLGSIIVGASSVDQLKENIENY
                     HTKVSLDQIKTARARVKDLEYTNHLV"
     gene            4262..4804
                     /locus_tag="POB55_001351"
     CDS             4262..4804
                     /locus_tag="POB55_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDI6500320.1"
                     /translation="MDLNEKTIDRTVIYNGKIVDVEIHTVTLPNGETSTRELVYHNGA
                     VAVCAVTPKKEVVLVKQYRKPVEKPLLEIPAGKLEDDEDRVEAAKRELEEETGYIAKE
                     LTHVVDMYGSPGFCDEQLSIYFTDNLEEGTVHLDEDEFVEVIKVPIENVKSMLMNKEI
                     EDAKTIIALQHLLLNYNHSK"
     gene            4909..5358
                     /locus_tag="POB55_001352"
     CDS             4909..5358
                     /locus_tag="POB55_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDI6500321.1"
                     /translation="MEERLNRVKQQLQQSSYKLTPQREATVRVLIENEKDHLSAEDVY
                     LKVKDKAPEIGLATVYRTLELLAELKVVDKINFGDGVARFDLRKEGAKHFHHHLVCME
                     CGRVDEIDEDLLPEVENRVENEFNFKILDHRLTFHGVCETCQAKGKG"
     gene            5407..6294
                     /gene="xerD"
                     /locus_tag="POB55_001353"
     CDS             5407..6294
                     /gene="xerD"
                     /locus_tag="POB55_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HDI6500322.1"
                     /translation="METIIEEYLRFIQIEKGLSSNTIGAYRRDLKKYQDYMTEHHISH
                     IDFIDRQLIQECLGHLIDQGQSAKSIARFISTIRSFHQFAIREKYAAKDPTVLLDSPK
                     YDKKLPDVLNVDEVLALLETPDLNKINGYRDRTMLELLYATGMRVSELIHLELENVNL
                     IMGFVRVFGKGDKERIVPLGDAVIEYLTTYIETIRPQLLKKTVTEVLFLNMHGKPLSR
                     QAIWKMIKQNGVKANIKKTLTPHTLRHSFATHLLENGADLRAVQEMLGHSDISTTQLY
                     THVSKSQIRKMYNQFHPRA"
     gene            complement(6372..6878)
                     /locus_tag="POB55_001354"
     CDS             complement(6372..6878)
                     /locus_tag="POB55_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HDI6500323.1"
                     /translation="MQQALINFYYQFHTKQHYFLCHDILEDAWKAENNYSKQDAVVSL
                     ILFATACYHYRRNNLKGAYKSFNKSKEIIQNAKDRDALYLNLNDYQLLIEQQIAKLNA
                     AKPFSSVILPITPDFERIIKANYPDYDYNQETSTDPFIVDHHMRRDRSEVISAKEEAI
                     QLRKHRRN"
     gene            6970..7701
                     /locus_tag="POB55_001355"
     CDS             6970..7701
                     /locus_tag="POB55_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HDI6500324.1"
                     /translation="MYEVKLDAFNGPLDLLLHLIQKFEIDIYDIPMQALTEQYMQYVH
                     AMKQLEINIASEYLVLASELLMIKSKMLLPQSTSDMDVDDDPREDLVGRLIEYQNYKE
                     YTAILNDMKEERDFYFTKRPTDLSHLETDESWDPNHTIDLTELIVAYQRVKNRVELNT
                     PKSVEIRKETFTIQQATEQVTSRLKDKDHFNFFSLFTFSEPIEQVVTHFLAILEMSKA
                     GIINIEQQRNFEDINIIRGVNYHFG"
     gene            7694..8236
                     /gene="scpB"
                     /locus_tag="POB55_001356"
     CDS             7694..8236
                     /gene="scpB"
                     /locus_tag="POB55_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HDI6500325.1"
                     /translation="MDNHGILESLLFTAGDEGLDEKQLLEILDMSKNQLVELIENYSS
                     HGLMIQRFGTTYVLTTKKEAATYIEQLIEQKSQMKLSQAAMEVLSIIAYNQPLSRSDI
                     ELIRSINSDGAVKTLIAKGLVEAKVVNEQRSQQLITTDLFLNVFGISNIEDLPTTEED
                     DEEMDAFFSNLVNQKGENND"
     gene            8229..8966
                     /locus_tag="POB55_001357"
     CDS             8229..8966
                     /locus_tag="POB55_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDI6500326.1"
                     /translation="MTKELERLQKRIANSGYTSRRKAETLISEGKVKVNGTTVTELGT
                     KVKPSDTIEVEGIKIELEDKIYILFHKPTQVITSVSDDRGRTVVTDYFKDIEARIYPV
                     GRLDYDTSGLLLLTNDGEFTNLMTHPRYQIKKKYVAKLKGYLMREEVKALEKGIELED
                     GMTQPAEVKVKKQDKDKNTTLVEITITEGRNRQVRRMFEHFGHQVTKLSRIEYGPLNV
                     VGLNAGEGRVLTPHEVKVMRHLAEHGK"
     gene            9099..9824
                     /gene="srrA"
                     /locus_tag="POB55_001358"
     CDS             9099..9824
                     /gene="srrA"
                     /locus_tag="POB55_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator SrrA"
                     /protein_id="HDI6500327.1"
                     /translation="MSNEILIVDDEDRIRRLLKMYLERESFEIHEASNGQEAYELAME
                     NNYACILLDLMLPEMDGIQVATKLREHKQTPIIMLTAKGEETNRVEGFESGADDYIVK
                     PFSPREVVLRVKALLRRTQSTTVEQSEPHARDVIEFKHLEIDNDAHRVLADNQEVNLT
                     PKEYELLIYLAKTPNKVFDREQLLKEVWHYEFYGDLRTVDTHVKRLREKLNRVSSEAA
                     HMIQTVWGVGYKFEVKSNDEPAK"
     gene            9805..11556
                     /gene="srrB"
                     /locus_tag="POB55_001359"
     CDS             9805..11556
                     /gene="srrB"
                     /locus_tag="POB55_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     SrrB"
                     /protein_id="HDI6500328.1"
                     /translation="MMSRLNSVVIKLWLTIILIVTTVLILLSIALITFMQYYFTQETE
                     NAIREDARRISSLVEQSHNKEEAIKYSQTLIENPGGLMIINNKHRQSTASLSNIKKQM
                     LNEVVNNDHFDDVFDKGKSVTRNVTIKEKGSSQTYILLGYPTKAQKNSHSKYSGVFIY
                     KDLKSIEDTNNAITIITIITAVIFLTITTVFAFFLSSRITKPLRRLRDQATRVSEGDY
                     SYKPSVTTKDEIGQLSQAFNQMSTEIEEHVDALSTSKNIRDSLINSMVEGVLGINESR
                     QIILSNKMANDIMDNIDEDAKAFLLRQIEDTFKSKQTEMRDLEMNARFFVVTTSYIDK
                     IEQGGKSGVVVTVRDMTNEHNLDQMKKDFIANVSHELRTPISLLQGYTESIVDGIVTE
                     PDEIKESLAIVLDESKRLNRLVNELLNVARMDAEGLSVNKEVQPIAALLDKMKIKYRQ
                     QADDLGLNMTFNYCKKRVWSYDMDRMDQVLTNLIDNASRYTKPGDEIAITCDENESED
                     ILYIKDTGTGIAPEHLQQVFDRFYKVDAARTRGKQGTGLGLFICKMIIEEHGGSIDVK
                     SELGKGTTFIIKLPKPE"
     gene            11801..12709
                     /locus_tag="POB55_001360"
     CDS             11801..12709
                     /locus_tag="POB55_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002955256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 domain-containing protein"
                     /protein_id="HDI6500329.1"
                     /translation="MKKFIGSVLTTTLILGGCSAMENESNKDTNTETKSVPEEMEASK
                     YVGQGFQPPAEKDAIEFAKKHRKEFEKVGEQFFKDNFGLKVKATNVVGKDDGVEVYVH
                     CEDHGIVFNASLPLYKDAIHQKGSMRSNDNGDDMSMMVGTVLSGFEYRAQKEKYDNLY
                     KFFKENEKKYQYTGFTKEAINKTQNVGYQNEYFYITYLSRNLKEYRKYYEPLIHKNDK
                     EFKEGMQQARKELDYTANTDAVSTLFSTKKNFTKDNTVDDVIELSNKLKEKPNMPQKS
                     QVTIQLGKSSINTKKPFYDDINPIEG"
     gene            12721..>14408
                     /locus_tag="POB55_001361"
     CDS             12721..>14408
                     /locus_tag="POB55_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500330.1"
                     /translation="MNKINDRDIAELSSYWVYQDINEKKDFKVNGKRFKQIDNYNDNS
                     NDNMNGAADIKTYELLNDQGNPTGQQTIIYQGTSNEAINPNNPLKSSGFGDDWLQNAK
                     LMNNDNESTDYLKQVEEFANSYRQKIKDASVLDENEFRRKYKTKPYNYKNKTIVADGG
                     NSEGGAGAKYQGAKHPNEKVVATDSAMIPYAAWQKFARPRFDNMISFNSTNDLLTWLQ
                     DPFIKDMPGKRVNISDGVPRLDALIDSHVGYKRKLNRKDNTYDTVPLIKIKSVKDTEI
                     KNGKKVKKTINITLDMDGRIPINVWTGDSIARSGKGTLIKLNLENLDALSKLITGETS
                     GMLAECVIFLNESFNISENENKNFADRKKQLSEGFKDKINLFQLEEMERTLISKINAL
                     EEVADETIESISAVKHLLPDFALDALKERINELFKGIKSFIEKVYDSIDNEILEIFKN
                     IDHDFRDGVSEEMMKHLKVVKQNIEQIKNQNDIYGRQIADIRSIMKQQDATILDGNYQ
                     INYSGENMVQGLALPSNYLGRKMKILKDHIDDGIKKIADYVQGIYDEYASKIVDV"
BASE COUNT         5298 a         1981 c         2643 g         4486 t
ORIGIN      
        1 aactaaattg atacaaattt tatgataaaa tgaattgaag aaaaggaggg gcatatatgg
       61 aagttacatt ttttggaacg agtgcaggtt tgcctacaaa agagagaaat acacaagcaa
      121 tcgccttaaa tttagaacca tattccaatt ccatatggct tttcgacgtt ggtgaaggta
      181 cacagcacca aattttacat catgcaatta aattaggaaa agtgacacat atatttatta
      241 ctcatatgca tggcgatcat atttttggtt tgccaggatt actttctagt cgttcttttc
      301 agggcggtga acagaagccg cttacattgg ttggaccaaa aggaattaaa gcatatgtgg
      361 aaatgtctat gaatttatca gagtcacatt taaactatcc aattacatat attgaaatcg
      421 atgatcatct gacatatcat catgatggtt ttactgtaga ggcgcattta cttaatcatg
      481 gtgttccatc atatggatat agagtaatgg cacctgaaac tacaggtaca atcaatgttg
      541 aagcgttaaa aaacattggt cttgaaccag gtcctaaata tcaagaagta aaatctcatg
      601 atacatttga acataatggt caagtttatc aatcaaaaga ttttagaggt gagtccaaac
      661 agggtccagt tgtagcaatt tttggtgata caaaaccatg ttctaatgaa cgtgtgattt
      721 ctagagatgc tgatgtgatg gtacatgaag cgacctatat agatggtgaa aagcatttag
      781 ccaacaatta tcatcatagc catattgaag acgtatttgc attgataaaa gaagccaatg
      841 ttaaacgtac gttgattaca catttgagca atcgctataa tacagaagat ataaatgaaa
      901 tttatcaaac attgatacaa aatgaagata cacctaattt taattttgta aaagattttg
      961 atagttttaa agtctaatga caaagtaata aacaatgctt gccaaatagt aataatttat
     1021 aaaaataacc cgtccaacta tgtatgcgat gatgcataca tgtgttggcg ggtttgtttt
     1081 tattggtctt ctacattaga aagttcaata ctacggtcga cggcagcatt tagacaatct
     1141 tcgaaaatag atactaaatc atattgtgac aatgtatcaa ggccagcttg tgtcgtacca
     1201 cctttagagg taatattttt tcttaattga gccatgctca aatctgaacg ttcaatcatc
     1261 ttacttgtac ctataataag gttgcgtata gattcttcaa cttgttcttt ttctagacca
     1321 agtttcgtac cagctttaac gtattgctcg aatacatgat ataaaaatgc tgggccgctt
     1381 ccggtgatag ctgttacttg atgtaaatga tcttctgata cttcaattac agaaccaaat
     1441 gctttaacta aatcgttaat ttcatcttta gatttagggt caaagttgtt tgaaaaacta
     1501 atgccagtaa cagagtgtcc gacttgcgca tttgtgtttg gcataattct agcaactgga
     1561 ttttggcatt ctaattgttg tttaatataa tcaatcggaa tacctgccat aattgaaatg
     1621 aagcaattgt cttttgtgat atgtggtttg atgcgtgttg ctagagcatc aaagtcatgt
     1681 ggtttggtac ctaaaaaaac ataatctgca tcttttaata atgtcgcatc atcataacta
     1741 tagttaacac ctagtttttc agcgaatgct tttaaagctt gttcattaga tttatttgtt
     1801 aaatatatat cattggcatc taagttgctt gagttaataa ttcctgtaaa tatagcttgt
     1861 gccatattac cagctccata aaatacgagt ttcattaacg tcacatccct ttagtttttg
     1921 atttgtatgt tatattttct tattattgta tatatattag gaaagataat caacttgtct
     1981 caattatgta aagttgataa attgcattct atataattga aaaggaaggt taattgatga
     2041 aaggacaaca ttttatagtg actggtggta cgagtggttt aggtttatcg attgtccgaa
     2101 agttactaga aaataaagtg catgtaacgt tgttggtgcg tgatgttgat aaagcaacta
     2161 gaatctttga acaggaattg gggaaaacta taaatgttat tccttgtgac ttaaatgatt
     2221 tgaagtcgat acaggcactg caatttgaag acaatacatc attcgatgga ttcatttata
     2281 gtgcaggttt aggttatttc aaatctattt cagaccatag tttttcagaa atgattgaaa
     2341 cgtatcagct taatttaatt agttttaatg ttttatatac tgtgttgaga ccatatttaa
     2401 catctaacgc acatattgta ggtatttcaa gtcaagctgc attttccaca caagcaaatg
     2461 ctgcacatta tggggcatcg aaagcagggt tttatgcatt aatgaatgct ttaagactag
     2521 agtcaccaaa cttacatatc atgacagtta atgttggccc gattgataca ccttttcatc
     2581 aaaaagccga cccatcaatg aagtatgcta aaaggatggg gaaaattatg cttgatgcta
     2641 atcagcttgc agaagatatc atttatggca ttaaaacaaa acaattggaa attaatcggc
     2701 ctaaatggat gcatcatgcg ttgaaaatgt atcaaattgc gcctagattt tttgaacgat
     2761 gtttccctaa attatttaaa aataaagcat aaataggtgg gatggcaatg aactgtcaat
     2821 cggagagtgt ggtaagattt tgtgtacgat atgcggaaca actatcagtg tttgaagaat
     2881 ttacagtgct ggatatttta ggcgatattt ctgtagacca aatttcagac agtacattat
     2941 attatacttg tgaaaaattc aaactacttg tgttgcaagg aaatgtgctg ggtgtacaaa
     3001 taataactaa taatgatgaa tcaacatgcg aagtgaaata tagaaagatg ttttaataaa
     3061 attattaaga gatgaagtgg cagaagtttg cttaaggcaa tgccgcaagg cgaaattact
     3121 aaatactgat gcgtgaggac aacgctttaa tttctatcat aagtgagtaa tcataatcat
     3181 aaattatagc gaattgtaca taaacaacag cagtaaggca tttttataat gaaacgcctc
     3241 atgctgctgt ttttattact gaaaatgact tctacactaa atgattggta tattccaaat
     3301 cctttacacg agctcttgct gttttaatct gatctaaact aactttagta tgatagtttt
     3361 caatattttc ttttaattgg tcgacgctac ttgcacctac aatgatggaa ccaagcacgt
     3421 catgtgatgt taaataacta aatgtcaatg cagataaatt actttcaatt tctttaatag
     3481 aggctattgt ttcacccaat tcatcatgag aataatcaaa aataccatct ttaaatttat
     3541 tgtcgagcac attaacactg tttgaagtta ataatccttt agacacaggt cctcttgcca
     3601 atactttaac atcattgttg tgaattgcat ctaataatga ttctggacga ttatcaatca
     3661 aattgaattg agacattatc gtttcgattt gactatgttt taaataataa tcaattacat
     3721 ttgggcgaat agaagaaata ccgtaagcac gtataattcc ttcttgtttc aattcatcaa
     3781 atgcgcttat tgtttcgtct aatgggtcat caatggttcc gccatgaagt tgatataaat
     3841 cgatatgatc gatacctaaa cgctttagtg aacctttaac tgcctcttta atataggatt
     3901 tactcggatc ccattttgta ctgccatctt ttgttaaacg attgcctact tttgtaccaa
     3961 tgacaatatc atcgcgttgt tgatatttaa gaagtgcctt accaacaact gattcattta
     4021 tacctttatc gtacatatct gctgtatcaa aataagtgat accattttca acagcacaat
     4081 ctataatttg ttcggctttt tttaaatctg tacctaaact catgcaacct agacctaatt
     4141 cagataatga aataccactt tttaatatat ttttttgcat gtctgttctc ctttcgatac
     4201 actttagcta acaagcatag tgtatcaata tataatatga atttaaagga acgtgattat
     4261 aatggattta aatgaaaaaa caattgatcg aacagttatt tataatggca aaattgtaga
     4321 tgtagaaatt catacagtga cattaccaaa cggtgaaaca tcaacaagag aattagttta
     4381 tcataatggt gcagttgctg tttgtgcagt gacacctaaa aaagaagtcg tattagtgaa
     4441 acagtatcgt aaaccagtag aaaaaccatt gctagaaatt ccagctggta aattagaaga
     4501 tgatgaagat agagtcgaag cggcaaagcg tgaattagaa gaagaaacag gatatattgc
     4561 caaagagtta acacatgttg tagatatgta tggttcacca ggtttttgcg atgaacaatt
     4621 atcaatatat tttacggata atttagaaga aggtacagtt cacttagatg aagatgaatt
     4681 tgtcgaagtc attaaagttc cgattgagaa tgttaaatcg atgctaatga ataaagaaat
     4741 agaagatgcg aagactatca ttgcattgca acacctatta ttaaattata atcattctaa
     4801 ataacaaatg agaaaagctt gcattttatt gagaaaactg ttagttttaa ttgtgaagtt
     4861 tgaaataatt tgtaatgatt ttaattatta gtaggggagt ggacatcgtt ggaagaacga
     4921 ttaaatcgcg ttaagcaaca attacaacaa tcatcatata agctaacgcc acaacgcgaa
     4981 gctactgtta gagttctaat tgaaaatgaa aaagatcatc taagtgctga agacgtatat
     5041 ctgaaagtaa aagataaagc gcctgaaatt ggcttggcga cagtatacag aacgttagag
     5101 ttgttagctg aactaaaagt tgtcgacaaa attaactttg gtgatggcgt cgctcgtttt
     5161 gatttaagaa aagaaggcgc aaaacatttc caccatcatt tagtatgtat ggaatgtggt
     5221 cgtgtagatg aaatcgatga agatttgtta ccagaagttg aaaatcgagt tgaaaatgag
     5281 ttcaatttta aaattttaga tcatcgttta actttccatg gtgtgtgtga aacgtgccaa
     5341 gctaaaggta aaggatagta aattgcgtag gttaaattaa ccttcgcttt ttttagaggt
     5401 gtggttatgg aaacaattat tgaagaatat ttgcgtttta tacaaattga aaaaggacta
     5461 agttctaaca caattggtgc ttatagacgt gatttgaaaa agtatcaaga ttatatgact
     5521 gaacatcata tctcgcatat tgattttata gatcgacaat taattcaaga gtgtttgggg
     5581 catttaatag accaagggca atctgctaaa tctattgcgc gatttatttc aacaatccgt
     5641 agttttcatc aatttgctat aagagaaaaa tatgcggcga aagatccaac ggtattatta
     5701 gattcaccaa aatatgacaa aaaattgcct gacgttttaa atgttgacga agtgttggct
     5761 ttattagaaa cgccggatct aaataaaatt aatggttatc gtgatcgtac gatgttagaa
     5821 cttctgtacg caacgggaat gcgtgtatct gaattgatac atttagagtt agaaaacgtg
     5881 aacttaataa tgggatttgt acgcgtattt ggtaaaggcg ataaagaaag aattgtacca
     5941 ttaggcgacg cagtcattga gtacttaact acttatattg aaacgattag accgcaactt
     6001 ttaaaaaaga ctgttaccga agtcttattt ttaaatatgc atggtaaacc tttatcacga
     6061 caagcaatat ggaaaatgat taaacaaaat ggtgtaaagg caaacattaa aaagacgtta
     6121 acgccacata cgttacgcca ctcttttgcg acacatttat tggaaaatgg cgcagattta
     6181 agagcagtgc aagagatgtt aggtcactct gacatatcta ctacgcaact ctatacacat
     6241 gtttcaaaat ctcaaattag aaaaatgtat aaccaatttc atcctagagc ataaagtgaa
     6301 ccataactca aaagtcacaa tacacatgac taaaaatgtc tgtgctattg tggctttttt
     6361 aaattggttg attaattacg tctatgtttt cttaattgaa tcgcttcttc ttttgctgaa
     6421 atcacttctg aacgatcacg ccgcatgtga tggtctacaa taaaaggatc tgttgatgtt
     6481 tcctgattat aatcatagtc tggatagttg gccttgatga tgcgttcaaa gtctggagtt
     6541 attggtaata taacagatga aaaaggcttt gctgcattca attttgcaat ctgttgctca
     6601 attaacaatt gataatcatt taaattaagg tataacgcat ctctatcttt agcattttgt
     6661 attatttctt tagatttatt aaaagactta taggcgcctt ttaaattatt gcggcgataa
     6721 tggtaacaag cagttgcaaa caagattaaa ctaacaactg catcttgctt actgtagtta
     6781 ttttcagctt tccatgcatc ttctaaaatg tcatgacata ggaaataatg ttgcttagta
     6841 tgaaattgat aatagaaatt tatcagtgcc tgttgcattt tgttatcacc ccaatttaaa
     6901 agtaagttat tttcatgcta taatatttta gagaattatg cacatatgac gcaatacgag
     6961 gtagatatta tgtatgaagt taaattagat gctttcaatg gaccattaga tttattactg
     7021 catcttatcc aaaaatttga aatagatatt tatgatattc ctatgcaagc attaacagag
     7081 cagtatatgc agtacgttca tgcaatgaaa cagcttgaaa ttaatattgc aagtgaatac
     7141 ctagtattag cgtcagaact cttaatgatt aaaagtaaga tgctattacc acaatcaaca
     7201 tcagatatgg atgttgatga tgacccacgg gaagatttag ttgggcgttt aatagaatat
     7261 caaaattata aagaatatac tgctatttta aatgacatga aagaagaaag agatttttat
     7321 tttactaaaa gaccgacaga tttatctcat ttggaaacgg atgaatcttg ggatccaaat
     7381 catacgattg atttaactga attaattgta gcttatcaaa gagttaaaaa tagagttgag
     7441 ttaaatacac ctaaatctgt tgaaatccga aaagagacat ttaccattca acaagctaca
     7501 gaacaagtga catcgagatt gaaagataaa gatcatttta acttctttag tctgtttacg
     7561 ttttctgagc caattgaaca agtagtcact cactttttag ctattttaga gatgtcaaaa
     7621 gcaggaataa ttaatattga gcaacaacgt aattttgaag atattaacat tattagagga
     7681 gtgaactacc attttggata atcatggtat attagagtcg ctattattta cagctggcga
     7741 tgaaggttta gatgaaaaac aactattaga aatattagat atgtcgaaaa atcaactcgt
     7801 tgaattaatt gaaaattatt catcacatgg attaatgata caacgatttg gaacgacgta
     7861 tgttttaacg actaaaaaag aagcggcaac gtatattgaa caattaattg aacaaaagtc
     7921 acaaatgaaa ttatcacaag cagcaatgga agttctatca attattgctt ataaccagcc
     7981 attatcaaga agtgatattg aattaattcg tagtatcaat tcagatggtg cggtcaagac
     8041 attgattgcc aaaggactag ttgaggctaa agtggtcaat gaacagcgta gccaacagtt
     8101 aattactact gatttatttt taaatgtatt tggtatttcc aatatagaag atttgccgac
     8161 aactgaagaa gacgatgaag aaatggatgc tttcttcagt aatctagtca atcaaaaagg
     8221 agaaaataat gactaaagaa ttagaacgat tacaaaaacg tatagctaat agcggttata
     8281 cttcaagacg taaggcagaa actttaattt ctgaaggaaa agtgaaagtg aatggtacga
     8341 ctgtcactga attaggaaca aaagtaaagc catcagatac gattgaagtt gaaggtatta
     8401 aaatagaact agaagacaaa atttacattt tattccataa accaactcaa gtgataacaa
     8461 gtgtatcaga tgatagagga cgtacagtgg ttacggatta ctttaaagat atagaagcac
     8521 gtatttatcc tgttggtcgt ttagactatg atacttctgg attgttatta cttacaaatg
     8581 atggtgaatt tactaattta atgacacatc caagatatca aattaagaag aaatatgtag
     8641 caaaattaaa aggttactta atgagagaag aagtgaaagc cctagaaaaa ggtattgaat
     8701 tagaagatgg tatgacgcaa ccggctgaag tgaaagttaa gaaacaagat aaagataaaa
     8761 acacaacatt ggttgaaatt acaattacag aaggtcgtaa tcgccaagtc agaagaatgt
     8821 tcgaacattt tggtcatcaa gtgacgaaac tgtctcgtat tgaatatggg cctttaaatg
     8881 ttgtcggttt gaatgctggt gaaggacgtg tattgacgcc tcatgaagta aaagtgatgc
     8941 gtcatttagc agaacatggg aaataattaa ataaaatatg tatttttcac aaagtttgag
     9001 aattttttca caaaatttta ggtttttaat aactgtatgc gctttcctgt gatataatac
     9061 ttttgtaaag aaaagcatgt gtgggaggta tgacctgtat gtcgaacgaa atacttatcg
     9121 tagatgatga ggatagaatc agaagattac ttaaaatgta tttagaaaga gaatcttttg
     9181 aaatccatga agcaagtaat ggccaagagg cttatgaact tgcaatggag aataattatg
     9241 cttgcatact actagattta atgttgcctg aaatggatgg tatccaggtg gcaactaaat
     9301 tgcgtgaaca taaacaaaca ccgattatta tgttgactgc taaaggtgaa gaaacaaacc
     9361 gtgttgaagg ttttgaatct ggtgcagatg attatatcgt caaaccattt tcaccaagag
     9421 aagtagtctt aagagttaaa gcacttctaa gaagaacgca atctacaact gtagaacaaa
     9481 gcgaacctca cgcacgtgat gtgattgaat ttaaacattt agaaatagat aatgatgcac
     9541 atcgcgtact tgctgataat caagaagtta atttgactcc taaagagtac gaattattaa
     9601 tatatttagc taaaacacca aataaagtat ttgaccgtga acaattatta aaagaagttt
     9661 ggcattatga attctatggt gatttaagaa cagttgatac tcatgttaaa cgacttagag
     9721 aaaagttaaa tcgtgtgtct agtgaagctg cgcatatgat tcaaacagtc tggggcgttg
     9781 ggtataaatt tgaggttaaa tctaatgatg agccggctaa atagtgtcgt aattaaactg
     9841 tggttaacta ttattttaat agtgacgaca gttttaattt tattaagtat tgctttaatt
     9901 acctttatgc aatactattt cacacaagaa accgaaaatg ccataagaga agatgctaga
     9961 cgtataagtt cactggtcga acaatcacat aataaagaag aagcaataaa atatagtcaa
    10021 acattaattg aaaatcctgg tgggttgatg attataaata ataaacatcg tcaatcaacg
    10081 gcttcacttt ctaatattaa aaagcaaatg ttgaatgaag tagtcaacaa cgaccatttt
    10141 gacgatgtgt ttgataaagg taaatctgtt actcgaaatg taacgattaa agaaaagggc
    10201 tcatctcaaa catatatttt gttaggctat ccaacaaaag cacagaagaa tagtcatagc
    10261 aaatatagtg gagtctttat atataaagac ttgaaatcaa tcgaagatac aaataatgct
    10321 attacgatta tcactataat tacggctgtt attttcttaa caattacaac agtctttgcg
    10381 tttttcttat catcaagaat tacaaaacct ttaagacgtt taagagacca agctacacgt
    10441 gtatctgaag gggattactc ttataaacct tctgtcacaa cgaaagatga aattggtcaa
    10501 ttatcgcagg catttaatca gatgagtaca gaaatcgaag agcatgtcga cgcattatcc
    10561 acatctaaaa atattagaga cagcttaatt aactctatgg tagaaggtgt cctaggtatt
    10621 aatgagagtc gacaaattat cttatctaat aagatggcga atgatattat ggacaatatt
    10681 gatgaagatg ctaaagcttt cttattaaga caaattgaag atacttttaa atcaaaacaa
    10741 acggaaatgc gtgatttaga aatgaatgca cgattctttg ttgtgaccac aagctatatc
    10801 gacaagattg aacagggagg taaaagtggt gttgttgtga cagttcgtga tatgactaat
    10861 gagcacaatc tagatcaaat gaagaaagat ttcattgcta atgtatcaca tgaattacgt
    10921 acaccgatat cattacttca aggttatact gaatcaattg tagatggtat tgttacagaa
    10981 ccggatgaaa taaaagaatc gcttgccatt gtccttgatg aatcgaaacg tttaaatcgt
    11041 ttagttaatg aattgttaaa tgtcgcacgc atggatgctg aagggttatc cgtaaataaa
    11101 gaagttcagc ctattgcagc gttactagat aagatgaaaa ttaagtatcg ccaacaagct
    11161 gatgatttag gtctaaatat gacttttaat tattgtaaga agcgtgtttg gagttatgat
    11221 atggatcgca tggaccaagt actaacgaac ttaattgata atgcatcacg ttatacgaaa
    11281 cctggagatg aaattgcaat tacttgtgat gaaaatgaaa gcgaagatat tttatacatt
    11341 aaagatacag gtacaggcat tgcaccagaa catttacaac aagtatttga tcgtttttat
    11401 aaagttgatg cagcgagaac gcgaggtaaa caaggtaccg gtttaggttt gttcatttgt
    11461 aaaatgatta tcgaagagca tggtggttcc atagatgtta aaagcgaatt agggaaaggc
    11521 acaacattta ttattaaact accaaaacca gaataaaatt gaatatagtt atttcagaac
    11581 gcatgttatt gattagagac tctaatttat agcatgcgtt ttttgattga tgtggggaat
    11641 tttgtatgtg gattagaact tagggttttt gcgaatatca actattaaat atattactaa
    11701 tttatacaaa aatataaagt ttgataaagt tatttatttg attataaaaa tagggtaaaa
    11761 tatagatata ttgtattaat taaattattc gaggtgtcat atgaaaaagt tcattggatc
    11821 agttttaacc acgacattaa ttttaggggg atgttccgcg atggaaaatg aatcaaataa
    11881 agacacaaac acagaaacaa aatctgtacc agaagaaatg gaagcttcaa aatatgtagg
    11941 ccaaggcttc caaccgcctg cagaaaaaga tgcgattgaa tttgcgaaga agcatcgtaa
    12001 agaatttgaa aaagtaggtg aacaattctt taaagataac tttggactaa aagttaaagc
    12061 tacaaatgtt gtaggtaaag atgatggtgt agaagtttat gtgcattgtg aagatcatgg
    12121 cattgtattt aatgcaagtc tacctttgta caaagatgcc atccatcaaa aaggatcaat
    12181 gcgcagtaat gacaatggtg atgatatgag tatgatggtg ggtacagtgc tgagtggctt
    12241 tgaatatcga gcgcaaaaag aaaagtatga taacttatat aaattcttca aagaaaatga
    12301 aaagaaatat caatatacag gctttacaaa agaggcaatt aacaagacac aaaacgttgg
    12361 atatcaaaat gaatactttt atatcacata tttatcaaga aacttaaaag aatatcgtaa
    12421 atattacgaa ccgttgattc ataaaaatga taaagaattt aaagaaggta tgcaacaagc
    12481 tagaaaagag ttagattata ctgccaatac tgatgcagtt tctacacttt tttcaactaa
    12541 gaaaaacttt actaaagaca atacagtaga tgatgtgatt gaattgagta ataaattaaa
    12601 agaaaaaccc aatatgcctc aaaaatctca agttactatt caattaggaa aatctagtat
    12661 aaatactaaa aaaccatttt atgacgatat aaatccaatt gaagggtaag tgaatataaa
    12721 gtgaataaaa taaatgatag ggacatagca gaattgagca gttattgggt ttatcaagat
    12781 ataaatgaaa aaaaagattt taaagtaaat ggaaaacggt tcaaacaaat agataattat
    12841 aatgataata gtaatgataa tatgaatggt gctgctgata ttaaaacata cgagttactt
    12901 aatgatcagg ggaatccaac tggtcaacag acaataattt atcaaggaac atctaatgag
    12961 gcaattaacc caaataatcc attaaaatca tcgggttttg gagatgattg gctccaaaat
    13021 gctaaattaa tgaataatga taatgaaagc acagattatt taaagcaagt agaagaattc
    13081 gcaaatagtt atcgacaaaa aattaaagat gcttctgttc ttgatgaaaa tgaatttaga
    13141 agaaaataca aaactaaacc ttataattac aaaaacaaaa caattgtggc ggatggtggt
    13201 aattcggaag gtggtgcagg agcaaaatat caaggagcga aacatccgaa tgaaaaagtt
    13261 gttgctactg actcagcaat gattccttat gctgcttggc agaaatttgc tagaccacgc
    13321 tttgataata tgattagttt taatagtacc aacgatttat taacatggtt acaagatcca
    13381 ttcatcaaag atatgccagg aaaacgcgtt aacattagtg atggtgtgcc caggttagat
    13441 gctttaatag acagccatgt aggttataaa aggaagttaa atagaaaaga taacacatac
    13501 gatactgtac cactaatcaa aattaagtcg gtaaaagata cagaaattaa aaatggaaaa
    13561 aaagtaaaaa agactattaa cataacatta gatatggatg ggcgaattcc aataaatgtt
    13621 tggacaggag attcgattgc acgttccgga aaaggaactt taattaaact taatttagaa
    13681 aatcttgatg cgttgagtaa actgattact ggtgaaacaa gtggtatgtt agcagaatgc
    13741 gtaatctttt taaatgaaag ttttaacatc tcagaaaatg aaaataaaaa ttttgcagat
    13801 agaaagaaac aattatcaga aggatttaag gataagatta acttatttca attagaagaa
    13861 atggaaagaa ctttaattag taaaataaac gcacttgaag aagttgcaga tgaaacaata
    13921 gaaagtatta gtgctgttaa acacttatta cctgattttg cattggatgc attaaaagaa
    13981 agaattaatg agttgtttaa aggtataaaa tcttttatag aaaaagtgta tgatagtata
    14041 gataatgaaa ttttagaaat tttcaaaaat atagatcacg acttcagaga tggagtatct
    14101 gaagaaatga tgaaacattt gaaagtagtg aaacagaata tagagcaaat aaaaaatcaa
    14161 aatgatattt atggtaggca aattgcagat attagaagta ttatgaagca acaagatgca
    14221 acaattttag atggaaatta tcaaattaat tatagcggtg aaaatatggt gcagggtcta
    14281 gctttacctt ctaattattt aggaagaaaa atgaaaatat taaaagacca tatcgatgat
    14341 ggtattaaaa aaatagcaga ctatgttcaa ggtatatatg atgaatatgc atcgaaaatt
    14401 gtcgatgt
//
LOCUS       DAMZKC010000063        14346 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.063, whole genome shotgun sequence.
ACCESSION   DAMZKC010000063 DAMZKC010000000
VERSION     DAMZKC010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14346
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.063"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(<1..303)
                     /locus_tag="POB55_001362"
     CDS             complement(<1..303)
                     /locus_tag="POB55_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDI6500331.1"
                     /translation="MKQLVGIPESMLIPLIARAKEYENEKPIIKDALSKKIFDGLDDM
                     YKNVTCDDMSQIGISIRTVIIDCVTKRLIKDNKDLIVVNIGCGLDTRFQRFNKEKIS"
     gene            complement(365..1276)
                     /locus_tag="POB55_001363"
     CDS             complement(365..1276)
                     /locus_tag="POB55_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-hydroxamate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDI6500332.1"
                     /translation="MKRLIGILLCNLFILTACSASVDKTSNSTKTTDYKIENGETLKV
                     PEKPKRVAVLTGFYVGDFIKLGIKPIAVSDITKDSSILKPYLKGVDYIGENDVERVAK
                     AKPDLIVVDAMDKNIKKYQKIAPTIPYTYNKYNHKEILKEIGKLTNNEDKAKKWIEEW
                     DDKTRKDKKEIQSKIGQATASVFEPDEKQIYIYNSTWGRGLDIVHDAFGMPMTKQYKD
                     KLQEDKKGYASISKENISKYAGDYIFLSKPSYGKFDFEKTHTWQNIEAVKKGHVISYK
                     AEDYWFTDPITLEHLRSKLKKEILNKK"
     gene            1437..2744
                     /locus_tag="POB55_001364"
     CDS             1437..2744
                     /locus_tag="POB55_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HDI6500333.1"
                     /translation="MNKVHKPLYFYLMLFFSTTIIGALLLYLPFTGKKPISFLDALFI
                     ASSAFTVTGLSPVDIGSQFNILGEIVILLLIQIGGLGIVTVTLLTLVFLNRKISMKNR
                     FLIMVTWNIDEPGGVIKLIKHLAIYSLVTELIGMICLCLSFIPKFGIGKGLFLSLFTS
                     VSAFNNAGFALFKNNLIDYSSDPIVIITISILIIFGGIGHFVVIDFINCKKLSKLSLH
                     SKLVLTTTSILIIIGAITFFLLEQFNTMQHMGLVEKIGNSFFQSVTTRTAGFNSIDIA
                     SINKSTALMLMLLMFIGGAPLSAAGGIKITTFAVAFIFVLNYIRKENNVSVFNKEISD
                     KHIKLSIVTINISFLFISIITFILSIINPNISLIKLLFEVVSAFGTVGLSMNLTTEYH
                     GITKIIIIFVMLCGKVGLLTLLRTFIPPKSPKNYRYTKGQIYL"
     gene            complement(3598..4041)
                     /locus_tag="POB55_001365"
     CDS             complement(3598..4041)
                     /locus_tag="POB55_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6500334.1"
                     /translation="MSIITRLFNNSDFEKLNQLCKLYDDLGYPTNENDLKKRLKKITN
                     HDDYFLLLLIKENKIIGLSGMCKMMFYEKNAEYMRILAFVIHSEFRKKGYGKRLLADS
                     EEFSKRLNCKAITLNSGNRNERLSAHKLYSDNGYVSNTSGFTKQL"
     gene            complement(4147..4584)
                     /locus_tag="POB55_001366"
     CDS             complement(4147..4584)
                     /locus_tag="POB55_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500335.1"
                     /translation="MFISLAIWSINSMGNKQCISLNLLLSDDTFAVISVMTTLGTAIL
                     GGIAPSIYGIFTSPIDKKSSFRAISDWEQEQRNNVKDLMMLLPFTLLSILIVIFIFQF
                     AYSKLFNTIAIIIFIIILVVMFFLILLICYKITVMICRYFSKE"
     gene            complement(4901..5491)
                     /locus_tag="POB55_001367"
     CDS             complement(4901..5491)
                     /locus_tag="POB55_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HDI6500336.1"
                     /translation="MSELTAKQARFVNEYIRTLNVTQSAIKAGYNANSAHVTACRLLK
                     KPHIKQYIQEQKDKIIDENVLTAKELLHVLTNATVGDETETKEVVVKRGEYKENPQSG
                     KVQLVYNEHVELIEVPIKPSDRLKAREMLGKYHKLFTDKHDINGNVPIFINIGEWDGG
                     DEGLDKAVKDVSNDNPNHTVIVDDIPLEDYESIRFL"
     gene            complement(5488..5700)
                     /locus_tag="POB55_001368"
     CDS             complement(5488..5700)
                     /locus_tag="POB55_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island family protein"
                     /protein_id="HDI6500337.1"
                     /translation="MTDDNVNDHIIKNHIEMIVDRLATDKEFYIFDSLIQGLSYQDIS
                     SALDCSEQSVILWYETILDKIVGVIK"
     gene            5761..6307
                     /locus_tag="POB55_001369"
                     /pseudo
     CDS             5761..6307
                     /locus_tag="POB55_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002476300.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(6402..8018)
                     /gene="groL"
                     /locus_tag="POB55_001370"
     CDS             complement(6402..8018)
                     /gene="groL"
                     /locus_tag="POB55_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500736.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDI6500338.1"
                     /translation="MVKQLKFSEDARQAMLRGVDQLANAVKVTIGPKGRNVVLDKEFT
                     APLITNDGVTIAKEIELEDPYENMGAKLVQEVANKTNEIAGDGTTTATVLAQAMIQEG
                     LKNVTSGANPVGLRQGIDKAVKVAVEALHENSQKVENKNEIAQVGAISAADEEIGRYI
                     SEAMEKVGNDGVITIEESNGLNTELEVVEGMQFDRGYQSPYMVTDSDKMVAELERPYI
                     LVTDKKISSFQDILPLLEQVVQSNRPILIVADEVEGDALTNIVLNRMRGTFTAVAVKA
                     PGFGDRRKAMLEDLAILTGAQVITDDLGLDLKDASIDMLGTASKVEVTKDNTTVVDGD
                     GDENSIDARVSQLKSQIEETESDFDREKLQERLAKLAGGVAVIKVGAASETELKERKL
                     RIEDALNSTRAAVEEGIVAGGGTALVNVYQKVSEIEAEGDIETGVNIVLKALTAPVRQ
                     IAENAGLEGSVIVERLKNAEPGVGFNAATNEWVNMLEAGIVDPTKVTRSALQHAASVA
                     AMFLTTEAVVASIPEKNNDQPNMGGMPGMM"
     gene            complement(8094..8378)
                     /gene="groES"
                     /locus_tag="POB55_001371"
     CDS             complement(8094..8378)
                     /gene="groES"
                     /locus_tag="POB55_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDI6500339.1"
                     /translation="MLKPIGNRVIIEKKEQEQTTKSGIVLTDSAKEKSNEGVIVAVGT
                     GRLLNDGTRVTPEVKEGDRVVFQQYAGTEVKRDNETYLVLNEEDILAVIE"
     gene            8553..9296
                     /locus_tag="POB55_001372"
     CDS             8553..9296
                     /locus_tag="POB55_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDI6500340.1"
                     /translation="MTRLWASLLTVIIYILSQFLPLLIVKKLPFVQYSGIELTKAVIY
                     IQLVLFLIAATTIILINLKIKNPTKLELEVKEPKKYIIPWALLGFALVMIYQMVVSIV
                     LTQIYGGQQVSPNTEKLIIIARKIPIFIFFVSIIGPLLEEYVFRKVIFGELFNAIKGN
                     RIVAFIIATTVSSLIFALAHNDFKFIPVYFGMGVIFSLAYVWTKRLAVPIIIHMLQNG
                     FVVIFQLLNPEALKKATEQANFIYHIFIP"
     gene            complement(9321..10463)
                     /locus_tag="POB55_001373"
     CDS             complement(9321..10463)
                     /locus_tag="POB55_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500341.1"
                     /translation="MSYHWFKKMLLSTSILILSSSSLGLATHTVEAKDNLNGEKPTTN
                     LNHNITSPSVNSEMNNNETGTPHESNQTGNEGTGSNSRDANPDSNNVKPDSNNQNPST
                     DSKPDPNNQNPSPNPKPDPDNPKPKPDPKPDPDKPKPNPNPKPDPNKPNPNPSPDPDQ
                     PGDSNHSGGSKNGGTWNPNASDGSNQGQWQPNGNQGNSQNPTGNDFVSQRFLALANGA
                     YKYNPYILNQINKLGKDYGEVTDEDIYNIIRKQNFSGNAYLNGLQQQSNYFRFQYFNP
                     LKSERYYRNLDEQVLALITGEIGSMPDLKKPEDKPDSKQRSFEPHEKDDFTVVKKQED
                     NKKSASTAYSKSWLAIVCSMMVVFSIMLFLFVKRNKKKNKNESQRR"
     gene            10660..11286
                     /locus_tag="POB55_001374"
     CDS             10660..11286
                     /locus_tag="POB55_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDI6500342.1"
                     /translation="MGLFKKDKKAMTFDNAMEERRSIYNLKDSISISDDELESMIAHA
                     VKHVPSSFNSQSTRIVLLLNDNNNKFWDNTKAILKEVMGENRDFEPTEQKIDNFKHSY
                     GTILFYEDQDVVSGLQEQMPNYYDNFAIWSTQTNAMHQFAIWTALATKGIGASLQHYN
                     PLVDEMTSNEFNIPKSWKLIAQMPFGDIREAAGEKTFNPVEDRFVIKK"
     gene            11647..12432
                     /locus_tag="POB55_001375"
     CDS             11647..12432
                     /locus_tag="POB55_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen family hydrolase"
                     /protein_id="HDI6500343.1"
                     /translation="MKVQIYQLPIIFGDSSKNEAQITQWFEKNMNAEVDVVVLPEMWN
                     NGYDLEHLNEKADNNLGQSFSFIKHLAEKYKVDIVAGSVSNIRNYQIFNTAFSVNKSG
                     QLINEYDKVHLVPMLREHEFLTAGENVAEPFQLSDGTYVTQLICYDLRFPELLRYPAR
                     SGAKIAFYVAQWPMSRLQHWHSLLKARAIENNMFVIGTNSTGFDGNTEYAGHSIVINP
                     NGDLVGELNESADILTVDLNLNEVEQQRENIPIFKSIKLDLYK"
     gene            complement(13031..13165)
                     /locus_tag="POB55_001376"
     CDS             complement(13031..13165)
                     /locus_tag="POB55_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lysin family phenol-soluble modulin"
                     /protein_id="HDI6500344.1"
                     /translation="MSCLILRIFILIKEGVISMAQDIISTISDLVKWIIDTVNKFTKK
                     "
     gene            13401..13964
                     /locus_tag="POB55_001377"
     CDS             13401..13964
                     /locus_tag="POB55_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator AgrB"
                     /protein_id="HDI6500345.1"
                     /translation="MNYFDNKIDQFATYLQKRNNLDHIQFLQVRLGMQVLAKNIGKLI
                     VMYTIAYILNIFIFTLITNISFYLIRRYAHGAHAPSSFWCYIESITLFIVLPLLVLHF
                     HINETLMMFLALISVGVVIKYAPAATKKKPIPARLVKQKRYFSIIISTILFIITLFVK
                     EPYTQFIQLGIIIQAITLLPIYYSKED"
     gene            13968..14108
                     /locus_tag="POB55_001378"
     CDS             13968..14108
                     /locus_tag="POB55_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HDI6500346.1"
                     /translation="MKKLLNKVIELLVDFFNSIGYRAAYINCDFLLDEAEVPKELTQL
                     HE"
BASE COUNT         4680 a         2423 c         1962 g         5281 t
ORIGIN      
        1 tgatattttt tctttattaa atctttgaaa ccttgtatct aagccacaac ctatattgac
       61 cacgattaaa tctttattat ccttgataag cctcttagta acacaatcta ttatcacagt
      121 acgtatacta attccaattt gagacatgtc atcacatgta acatttttgt acatatcatc
      181 taaaccatca aatatttttt tagatagtgc gtcttttatt attggttttt cgttttcgta
      241 ctcttttgct cgagctatca aaggaattaa cattgattcg ggaattccaa ccagttgctt
      301 catttaatca ctccttaaaa acattagtac caatgcataa gaaaattttt acaacttatt
      361 tctattattt tttatttaaa atttcttttt ttaatttact tctcaaatgt tctaatgtaa
      421 taggatctgt gaaccaataa tcttctgctt tatatgaaat tacatgtcct ttttttacag
      481 cttcaatatt ctgccatgta tgtgtttttt caaaatcaaa ttttccgtac gaaggtttac
      541 ttaaaaatat ataatcacca gcatatttac taatattttc ttttgaaatg gaagcatagc
      601 cttttttatc ttcttgtaat ttatctttat attgctttgt cattggcata ccgaatgcat
      661 catgaacaat atccaaacca cgaccccatg tagagttata tatgtatatt tgcttttcat
      721 ctggttcaaa cacagatgct gttgcttgac caattttact ttgaatttct tttttatctt
      781 ttctagtttt atcgtcccac tcttcaatcc attttttagc tttatcttca ttattagtca
      841 acttgcctat ttcttttaat atttctttat gattgtattt attgtatgta tatgggattg
      901 ttggcgctat tttttggtat ttttttatat ttttatccat agcatctaca acaattaaat
      961 ctggttttgc tttagcaact ctttcaacat cattttctcc aatataatca acccctttta
     1021 aataaggttt taaaattgaa gaatctttag ttatatctga aacagcaatt ggtttgattc
     1081 ctaactttat aaaatcacca acataaaatc cagttaatac agcaactctt ttaggtttct
     1141 ctggtacttt cagtgtttca ccattttcaa ttttatagtc tgtagtcttt gtcgagttac
     1201 ttgttttatc gacagatgct gaacacgcag tcaaaataaa taaattacat aataaaattc
     1261 caattaatct tttcataata tcatcctttt gttttttaat tgatattcat tttcaattat
     1321 aaaatattaa aagaattagt caacgcctgt cagtaataca catcagtaac atttctattt
     1381 tcatttatga tattatctaa ttattaattt ataatataaa ggagtatgca attaaaatga
     1441 acaaagtcca caaaccttta tatttttatt taatgctatt cttctctaca actatcatag
     1501 gtgcactttt attgtattta cctttcactg gtaaaaagcc aatatctttt ttagatgccc
     1561 tatttatagc ttcaagtgca tttacagtta ctggcctatc tccagttgat atagggtcac
     1621 agtttaatat acttggtgaa atagtaatac tattattaat tcaaataggt ggtctgggta
     1681 tcgtaaccgt aaccctattg acactagtat ttttaaatag aaagatatca atgaaaaata
     1741 gattcttgat tatggttaca tggaatattg acgaacctgg tggtgttatt aagctaatta
     1801 aacacttggc tatttatagt ttagtcactg aattaattgg tatgatttgt ttgtgtttat
     1861 cttttatacc aaaatttggt ataggcaaag gattattttt aagtttattc acatcagtat
     1921 cagcttttaa taatgccgga tttgcccttt ttaagaataa cttaatagat tattctagtg
     1981 atccaattgt cattattaca atctcaatac tcataatatt tggaggtatt ggacattttg
     2041 tcgtaataga ctttattaat tgtaaaaaat tgagtaaatt atctttacat tctaaattag
     2101 tcttaactac aactagtatc ctaataatta taggagctat tacattcttt ttattagaac
     2161 agtttaatac tatgcaacat atgggactag ttgaaaaaat cggaaattct tttttccaat
     2221 cagtaacaac acgaacagcg ggttttaaca gtatagatat agcaagcatt aacaaatcta
     2281 ccgccttaat gttaatgcta cttatgttta ttggtggtgc ccctctcagt gcagctggag
     2341 gaattaaaat aactactttt gcagtggcgt ttatatttgt tctaaattat atacgtaaag
     2401 aaaataatgt ttcagtattc aataaagaaa tatctgacaa acatataaaa ctatctattg
     2461 ttaccattaa tatctcattt ctatttatca gtatcattac ttttatatta tcgataatta
     2521 atccgaacat atcattaatc aagttattat tcgaagtggt ttctgcattt ggaacagtcg
     2581 ggttaagtat gaaccttacc acagaatatc atggtattac taaaataatt attatattcg
     2641 ttatgctttg cggtaaagta ggactattaa ctttattaag aacatttata ccaccaaaaa
     2701 gtcctaaaaa ttaccgctac actaaaggac aaatttatct ataaaatagc ttatccatct
     2761 ttaacaagaa atgaactagt gttttaatct attacattaa aaaaactacc taaacaggta
     2821 gttttttatt tattttaggc ccattaaatg tcttatgtct ttaattaagt aaaatgcaca
     2881 agtacacctt taaaaagatt tactcaaaac taaactatac ctagttaacc gcttgtgatt
     2941 aagaaatttt aaatgtggtt tcttttaatt atgtcaacca cccataagga ttttaatcaa
     3001 gcactcattc attcctgcac tcgcaatatg cattacttat agaagtggat gcactctcat
     3061 attatggcaa taccataact cccgcaccat aatgatttaa ataccattat gaatctcaat
     3121 tacaaattag cacataaaaa agacgcctcc cataagtcta gtcaccaaat ggaaaacgtc
     3181 tcgtaattaa aatatatttg cgtgcattct aatttgtact tagaatacat catactcgtt
     3241 cataatgtaa ttttctaatt agtcaaaact atatacagtt ttacatcatt cccggcatta
     3301 tgcactaata aaaacttact ttaatacacg ctttaaagat tattaaatca gcgttttaat
     3361 ttttagacaa cgctattatt tttagttttt caattctatt atgtcataat tatgtcactc
     3421 aaaaactgtt ttccaacatt gatttattgt tggaaaatct cgaaaatcta gtccatttct
     3481 ccaacaatcg aagaattatg cccttttttc tccctttaaa taagtcataa tacgaggcat
     3541 acatgcaaca tttacaataa aatatgttcc tacatcgtat tatacgaatg ctcacactta
     3601 tagttgttta gtaaacccag atgtattgct aacataccca ttatcactat atagtttatg
     3661 tgcagatagt ctttcatttc tattaccact atttagtgtt attgctttac aattcaaccg
     3721 tttagaaaat tcttcagaat cagctaataa tctctttcca taaccttttt tcctaaattc
     3781 agaatgtata acaaacgcaa ggattctcat atactctgca tttttttcgt aaaacatcat
     3841 tttacacata ccacttaaac caattatttt attttctttt atcaaaagca gtaggaagta
     3901 atcatcatga ttcgttattt tctttagtct cttttttaaa tcattctcat ttgttggata
     3961 acctagatca tcatataatt tacatagttg atttaatttt tcaaaatcac tgttattaaa
     4021 caatcttgta attatgctca tataacccac cctaaaaata ttatgtacct attgttacat
     4081 acaatagata cgcaaaatca tttctcactg caaatcagga cgtttctcag cgtaaacaaa
     4141 tcattgctat tcttttgaaa aatatctgca tatcatcaca gtaattttat aacagattaa
     4201 taaaattaaa aaaaacataa caacaagtat tataataaat attataatag ctatcgtatt
     4261 aaatagttta gaatatgcaa actgaaaaat aaaaatcaca attaaaatac taagtaatgt
     4321 aaatggtaat agcatcatca gatctttaac attatttctt tgttcttgct cccaatcgct
     4381 aatagcccta aaactacttt ttttatctat aggcgatgta aaaataccat atattgaagg
     4441 tgctatacct cctaaaatag cagttcccaa agttgtcatt actgaaatta ctgcgaaagt
     4501 atcatccgaa agcaataaat tcaaactaat gcattgttta ttacccatcg aatttattga
     4561 ccaaatagct agagaaataa acaacccaaa atttaaaata aatgatatag taatagcaat
     4621 tgttgacaaa acacgaaatt tttcattttt atttatatta tccattttct ccctttttct
     4681 taaatcattt tattatatat tacaataatt aatctgaaat gttgatgtaa tttgaaaaaa
     4741 atatcatact tttactcctg aaaacctccc taaatcatca atatgaaaat cagcattagg
     4801 atattgcgtt tacaactctt ttaaatctca ctcattcttc tcatcgtctt aaccgatact
     4861 atcaataaat actggcaaca ccataaaata tatacccaat ttataaaaac ctaattgact
     4921 cataatcttc taacggtata tcatccacaa tcacagtatg attaggatta tcgttagata
     4981 catctttcac tgccttatct aacccctcat cacctccgtc ccattcacca atattaatga
     5041 atataggtac attaccgttt atatcatgct tatctgtaaa taatttatga tatttaccca
     5101 acatttcacg agcttttaaa cgatcactag gttttattgg tacctctatc agttcaacat
     5161 gttcattata gactaattgt actttgccac tttgtggatt ttctttatat tctccacgct
     5221 tgactacaac ttctttcgtt tctgtttcat caccgactgt cgcattcgtt agcacatgta
     5281 ataactcttt tgcggttaat acattctcat ctataatctt atctttttgt tcttgtatat
     5341 attgcttgat gtgcggcttt ttcaataacc tacacgctgt cacatgtgcg ctatttgcgt
     5401 tatatcctgc ttttatggca ctttgtgtta cattcagtgt tctaatatac tcattcacaa
     5461 aacgtgcttg ctttgcagtt aactcactca ttttatcacc cccacaattt tatctaatat
     5521 ggtttcatac cataatatta cagattgttc tgaacaatct aaggcactac taatatcttg
     5581 ataactaagt ccttgtataa gggagtcaaa aatataaaac tctttatcgg tcgctaatct
     5641 gtcaacaatc atttctatgt gattctttat aatatgatca ttgacattat cgtctgtcat
     5701 caattcgtca gaatcttcat cacctattaa aaagaaatca tcagtattta tttcatcatc
     5761 gccccgttaa ctagctttga agtctttagc acacttgcat ataccggctg tcgtgctggc
     5821 gagatactag cattgaagtg gtctgatatt gattttgaaa acaacacgat tagtattact
     5881 aaaacatatt acaatccaaa taataacaag aagaaatttc agatacttcc ccctaaaact
     5941 gaaagttcta tcggtaagat ttccgttgac ccaaatgtaa taaaggtgtt gcgtgattat
     6001 aagataaacg ttcaaaataa ttggaaaaac gaattatata atgataacca ttgtaatgaa
     6061 aaaactatca atgtggatac aaaccattat gtcacgtacc agcatactca aaaatatctc
     6121 tacgcactca ttcagataca cacattgcgc attgctcata gaatcaggtg tacacattaa
     6181 agaaatacaa gaacgattac ggcataaaga tatcaatacc actatgaata tctatgctaa
     6241 aatcacaaat tcatacaaaa aagacgcctc ccataagttt agtcaccaaa tggaagacgt
     6301 ctcgtaatta aaatatattt gcgtgcattc taatttatac ttagaatgaa tcatactcgt
     6361 gcataatgta attttctagt tagtcaaaac tataaacagt tttacatcat tcctggcatg
     6421 ccacccatgt taggttggtc attatttttt tctggaattg atgctacaac cgcttcagtc
     6481 gttaagaaca ttgctgcaac acttgctgca tgttgtaatg ctgagcgtgt tactttagtt
     6541 ggatcaacga tacctgcttc taacatatta acccactcgt ttgtagcagc gttaaaacca
     6601 acacctggtt ctgcattttt caagcgttca acgatgacag agccttctaa tcctgcattt
     6661 tcagcaattt ggcgaactgg tgcagttaat gctttaagta caatattcac acctgtttca
     6721 atgtcgcctt cagcttcaat ttcacttact ttttggtaaa catttactag tgcagtacca
     6781 ccacctgcaa caataccttc ttcaactgct gcacgtgtag agtttaaagc atcttcaata
     6841 cgtaatttac gttctttaag ttctgtttca cttgctgcac ctaccttgat aactgcaaca
     6901 ccgcctgcta atttagctaa gcgctcttgt aatttttcac gatcaaagtc agattcagtt
     6961 tcttcaattt gagatttcaa ttggctaaca cgtgcatcaa tgctgttttc gtcaccgtca
     7021 ccatcaacaa cagtggtatt atctttagtt acttctactt tacttgcagt acctaacata
     7081 tcaattgatg catcttttaa atctaagcct aaatcatcag taatcacttg cgcaccagtt
     7141 aaaatagcta aatcttcaag catcgctttt ctacgatcac caaaaccagg tgcttttact
     7201 gcaacagctg taaatgtgcc acgcatacgg tttagcacga tatttgttaa tgcatcgcct
     7261 tcaacttcat cagctacaat taagattgga cgattagatt gaaccacttg ttctaataaa
     7321 ggtaagatat cttggaaaga cgagattttc ttatctgtta ctaaaatgta tgggcgttct
     7381 aattcagcaa ccattttatc tgaatcagta accatatacg gtgattgata accacgatca
     7441 aattgcatac cttcaaccac ttctagttca gtgtttagtc catttgattc ttcaattgta
     7501 atgacaccat cgttacctac tttttccata gcttcagaaa tataacgtcc aatttcttca
     7561 tctgctgctg aaatcgcacc tacttgcgca atttcatttt tattttcaac tttttgagaa
     7621 ttttcatgta acgcttcaac agcaacttta actgctttgt cgataccttg tcgtaaacca
     7681 actgggttcg caccacttgt aacatttttc aagccttctt gaatcattgc ttgagctaat
     7741 actgttgcag ttgtcgtacc gtcaccagca atttcatttg tcttatttgc gacttcttga
     7801 actagtttag ctcccatatt ttcatatgga tcttctaatt cgatttcttt agcaatcgtt
     7861 acaccatcgt tcgtaattaa aggtgctgta aactctttat ctaatacaac attacgtcct
     7921 ttaggaccaa tcgttacttt aactgcattt gcaagttggt caacaccacg taacattgct
     7981 tgacgcgcat cttcagagaa tttcaattgt ttaaccataa atgataaacc tccatttgtt
     8041 tagtcatatt tcattttcgt tctttttaat ttatctatga atttagtttt gtattattca
     8101 ataactgcta aaatatcttc ttcatttaat actagatatg tttcattatc tcgtttaact
     8161 tctgtaccag catattgttg gaacacgaca cggtcccctt ctttcacttc aggagtcact
     8221 cttgtaccat catttaatag acgtccagtt cctactgcaa cgataacgcc ttcgtttgat
     8281 ttttctttag cactatcagt taaaacaata ccacttttag ttgtttgttc ttgttctttt
     8341 ttctcaataa tcacacgatt tccaattggt tttagcatga ttgttcctcc ttaaaaaacc
     8401 taaagtttag cacttaacat taaagagtgc taacatacat ttataataat caaatttggt
     8461 caaaatttca agtcataacc ttttaattaa gttttgtaac ttcaagttat ttacgataaa
     8521 ataaattata aacaaatatt tggaggaaaa ttatgacaag attatgggca tcattgctaa
     8581 ctgttattat ttatatattg tctcaatttt taccgcttct cattgtaaaa aaattaccat
     8641 ttgtacaata tagtggcata gaactgacta aagcagtcat ttacatacaa cttgttctat
     8701 ttttaatcgc cgccacgacg attattttaa ttaatttaaa aattaaaaat ccaacaaaat
     8761 tagaattaga agttaaagaa cctaaaaaat atatcattcc atgggcattg cttggatttg
     8821 cattggtaat gatttatcaa atggtagtga gcattgtatt aacgcaaatt tatggtggac
     8881 aacaagtaag tcctaataca gaaaagctaa ttattattgc tcgaaaaata cctatattta
     8941 tcttctttgt atctattatt ggtcctttat tagaagaata tgtattcaga aaagtaatct
     9001 ttggagaatt atttaatgcg attaaaggta atcgtatcgt ggcatttatt attgctacaa
     9061 cagtaagttc attaatattt gcattagcac ataatgattt caaatttatt ccagtttatt
     9121 ttggcatggg tgtcattttt tcattagcat atgtttggac aaaacggctt gctgttccaa
     9181 ttattatcca tatgttacaa aacggatttg tcgttatatt ccaattactt aatcctgagg
     9241 ctttgaaaaa agccacggaa caagcgaatt ttatatatca catttttatt ccataataaa
     9301 aaaaacgaat cacacacgga ttatcgtcgc tgtgattcgt ttttattttt ctttttattt
     9361 cgctttacaa ataagaatag catgattgaa aataccacca tcatagaaca tacaattgct
     9421 agccaacttt tactatatgc agttgacgca cttttcttat tatcttcttg ttttttaact
     9481 actgtaaaat cgtctttttc atgcggttca aatgagcgtt gttttgaatc cggcttatct
     9541 tcgggctttt tcaaatctgg cattgatcca atttcaccag taattaatgc gagtacttgt
     9601 tcatctaaat tacgatagta cctttctgat ttcaatggat tgaaatattg gaatctaaag
     9661 taattcgatt gctgttgtaa tccatttaaa tatgcatttc cgctgaaatt ttgttttcga
     9721 ataatattat aaatgtcttc atcagtaact tctccataat ctttgcccaa cttattaatt
     9781 tgatttaaaa tatacggatt atacttgtaa gccccatttg ccaaggctaa aaatcgttgg
     9841 gatacaaaat cattaccagt aggattttgt gagtttcctt gattcccatt tggttgccat
     9901 tgaccttgat tagatccatc tgaagcattt gggttccatg tccccccatt tttcgagcca
     9961 ccagaatgat tggaatcccc aggttgatcg ggatctggtg acgggttagg atttggctta
    10021 ttagggtctg gttttggatt cggatttggc tttggtttat ctgggtctgg ttttggatcc
    10081 ggttttggtt tcgggttatc tggatctggt ttaggattcg gacttgggtt ttggttattt
    10141 gggtctggtt ttgaatctgt acttgggttt tggttgtttg agtctggctt cacattattc
    10201 gaatcaggat tagcatcacg actattcgaa cctgttcctt cattacccgt ttgatttgat
    10261 tcgtgaggtg tcccagtctc attattattc atttcactat ttactgatgg tgaagttata
    10321 ttatgattca aattagtagt tggtttttct ccatttaagt tatcctttgc ttcaactgtg
    10381 tgcgttgcaa gccctaaact actactactt aaaattaaaa tacttgttga aagtaacatt
    10441 ttcttaaacc aatgatatga catttcacga gacacctccc tttttcatgt aagtattaat
    10501 ctaagtataa taaatatgcg ttgaaaatgc atttatattt ggaatttgaa aatgaatatt
    10561 aatattcttg aaattttcgt aaatattgct ttacaagtac gagtataaat tttatactta
    10621 gtctattaag tattctttac taaagaagga gcaataatta tgggcttatt taaaaaagat
    10681 aaaaaagcaa tgacttttga caatgcaatg gaagaaagac gttcaatata taatttgaaa
    10741 gattcgatat caattagcga tgacgaatta gaatcaatga ttgcacatgc agttaaacat
    10801 gttccttcat catttaattc tcaatcaact agaattgttc tcttgttaaa tgacaacaat
    10861 aataaatttt gggataacac aaaagcaatt ttaaaagaag tcatgggaga gaatcgtgat
    10921 ttcgagccaa ctgaacaaaa aattgataac ttcaaacatt catacggtac aattttgttt
    10981 tatgaagatc aagatgttgt aagtggttta caagaacaaa tgccaaacta ctatgataat
    11041 tttgcaatat ggtcaactca aacaaatgcc atgcatcaat ttgcaatttg gactgcatta
    11101 gctactaaag gcataggtgc ttcattacaa cactataatc cacttgttga tgagatgact
    11161 tcaaatgaat ttaatattcc taaaagttgg aaacttattg ctcaaatgcc atttggtgat
    11221 attcgtgaag ctgctggcga aaaaacattt aatccagtgg aagatcgttt cgtaataaaa
    11281 aaataatcaa tatattgtta ggaatgggac agaaatgata aagaatcatt agttattcat
    11341 tatgtagtag ttcttacaca ttagccgctg ctaatgtgca cttaagaata ggaatacatg
    11401 agtaaaactc atgtataaga aatactaatt tctaaagaaa aagtatttct ttatgttaga
    11461 gcccacccca acttgcattg tctgtagaaa ttggtgttcc aatttctcta tgttggggcc
    11521 ccatcaacta ctgtcaatat aacattgtag agcctaggac tttgatttta tgtcctgggc
    11581 tcgttttttg cgtttcaaca taacttttag taagttatat ataagatcta tcaaggatgt
    11641 gatgttatga aagtccaaat ttatcaatta ccgattattt ttggagatag ttctaaaaat
    11701 gaagctcaaa taacgcaatg gttcgaaaaa aatatgaatg ctgaagtaga tgtagtcgtt
    11761 ttacctgaaa tgtggaataa tggctatgat ttagagcatt taaatgaaaa agcagataat
    11821 aatctaggac aaagtttttc atttattaaa catttagctg aaaaatataa agtcgatatc
    11881 gtagctgggt cagtttctaa cattagaaac tatcaaatat ttaatacagc ttttagtgtg
    11941 aataaaagtg gccagctaat taatgaatac gacaaagttc atttagttcc tatgttaaga
    12001 gagcatgaat ttttaactgc tggcgaaaac gtagctgaac cctttcaatt gtctgacggt
    12061 acttatgtga cccaacttat ctgttatgat ttacgttttc ccgaattatt aagatatcct
    12121 gctcgcagtg gtgctaaaat tgcattttat gtggcgcaat ggcctatgtc acgtcttcaa
    12181 cattggcatt cattactaaa ggcacgtgca attgaaaata atatgtttgt cattggaact
    12241 aatagcactg gattcgatgg taacacagaa tatgctggtc attcgatagt tataaaccca
    12301 aatggagatt tagttggaga attaaatgaa tccgcagata ttttgactgt agatttaaac
    12361 ttaaatgaag tagaacagca acgcgaaaat atacctatat ttaaaagtat taaattagat
    12421 ttgtataaat aagaagcgcc cgaaataata attaacacat gtcatgacta aacatagatt
    12481 tatgagagat ttttcataag tctatgttta tttttttata gtagataact cagtaagaat
    12541 ccatttcgcc ctttgcaaat gaatgatttt cttaattaaa ttgattaatt ttttgacctt
    12601 ttccaacatt agacttattc atatatttta acggtggatc tcataatgag gattaactct
    12661 tcccttcttt attacaataa aaaggccgcg agcttgggag gggctcacga ccatacttat
    12721 tattaaggga atgttttaca gttatttttt caatctattt ttggggatgt tattaattat
    12781 gaaaaaaatt ttatttatca aataatgata aatcgatgtt gtttacgata gcttacatgc
    12841 tagaaataat tatctgtaat gataattaag aaaaatacat agcactgagt ccaaggaaac
    12901 taactctact agcaaatgtt actcacttgc taattttaca tggttattaa gttgggatgg
    12961 cttaataact catactttta gtactatacg aagataacaa atttacaatg aaagtaatta
    13021 attattcatc ttatttttta gtgaatttgt tcactgtgtc gataatccat tttactaagt
    13081 cactgattgt tgaaatgata tcttgtgcca ttgaaatcac tccttcctta attaagataa
    13141 aaattcttaa aattaaacaa ctcatcaact attttccatc acatctctgt gatctagtta
    13201 tattaaaaca tgctaaaagc atttattttc caatttttct taactagtcg ttttttattc
    13261 ttaactgtaa atttttttat gttaaaatat taaatacaaa ttacatttaa cagttaagta
    13321 tttatttcct acagttaggc aatataatga taaaagattg tactaaatcg tataatgaca
    13381 gtgaggagag tggtgtaaaa ttgaattatt ttgataataa aattgaccag tttgccacgt
    13441 atcttcaaaa gagaaataac ttagatcata ttcaattttt gcaagtacga ttagggatgc
    13501 aggtcttagc taaaaatata ggtaaattaa ttgttatgta tactattgcc tatattttaa
    13561 acatttttat cttcacttta attactaata tatcgtttta tctaattaga aggtatgcac
    13621 atggtgcaca tgcaccttca tctttttggt gttatataga aagcattaca ctttttattg
    13681 tgctacctct attagtattg cattttcata ttaatgaaac actaatgatg tttttagcat
    13741 taataagtgt tggagtagtg attaaatatg cacctgcagc cactaagaag aagcctatcc
    13801 cagcgaggct tgtaaagcaa aaaagatatt tttcaattat aattagtaca attcttttca
    13861 ttatcacact ttttgtaaaa gaaccatata cccaatttat tcaactgggt attattatac
    13921 aagctattac attactacca atatactatt caaaggagga ctaaattatg aaaaaattac
    13981 tcaacaaagt tattgagctt ttagttgact ttttcaacag catcggttac agagccgcgt
    14041 atataaattg tgatttttta ttggacgaag ctgaagtacc aaaagaatta actcaattac
    14101 acgaataact ataagagaaa gtgtgatagt aagtggaagc attaaatgat tataattacg
    14161 ttttatttgt aattgttcaa gtttcattaa tgttctttat atctgcattt attagtggaa
    14221 taagatacaa aaaatcagat tatatataca ttattggaat agtgttatct tcagtatatt
    14281 tctttgacaa aatcggaagt atttcactag ttgttataac aatttttata ataatttttc
    14341 tctatt
//
LOCUS       DAMZKC010000064        14168 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.064, whole genome shotgun sequence.
ACCESSION   DAMZKC010000064 DAMZKC010000000
VERSION     DAMZKC010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14168
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.064"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            1..600
                     /locus_tag="POB55_001379"
     CDS             1..600
                     /locus_tag="POB55_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HDI6500347.1"
                     /translation="MAFKLPNLPYAYDALEPYIDQRTMEFHHDKHHNTYVTKLNATVE
                     GTELEHQSLADMIANLDKVPEAMRMSVRNNGGGHFNHSLFWEILSPNSEEKGGVIDDI
                     KAQWGTLDEFKNEFANKATTLFGSGWTWLVVNDGKLEIVTTPNQDNPLTEGKTPILLF
                     DVWEHAYYLKYQNKRPDYMTAFWNIVNWKKVDELYQAAK"
     gene            968..1693
                     /gene="sasD"
                     /locus_tag="POB55_001380"
     CDS             968..1693
                     /gene="sasD"
                     /locus_tag="POB55_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070002951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-wall-anchored protein SasD"
                     /protein_id="HDI6500348.1"
                     /translation="MKKLATVGSLIVTSTLVFSSMPFQNAHADTTSMNVSNKQSQNVQ
                     NHRPYGGVVPQGMTQAQYTELEKALSQLSAGSNMQDYNMKLYDATQNIADKYNVIITT
                     NVGVFKPHAVRDMNGHALPLTKDGNFYQTNVDANGINHGGSEMVQNKTGHMSQQGHMN
                     QNTHMNQQPHMQQGHMQSSNHQMMSPKANMHSSNHQMNQSNKKVLPAAGESMTSSILT
                     ASIAALLLVSGLFLAFRRRSTNK"
     gene            complement(1884..2639)
                     /locus_tag="POB55_001381"
     CDS             complement(1884..2639)
                     /locus_tag="POB55_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDI6500349.1"
                     /translation="MEVVYVTQTQPKFLTIYNTLYKEIQIGKYPSGHALPTEKELCAR
                     FDVSRMTLRQAIKLLAEDGIVESTRGKGHFVIPQTNAQHASSMDQFKHPLDQILLARV
                     SMSSINYRVDLESEYTNHLFANHPSAVIAMERYYQKKDNHSKQADAFCFTFIPLNVID
                     TFKVNTQNEDDMKTFVEQTIYSNAYQSDLKMSITKAPHFKNHSHVFDGDTHCWLIIET
                     LYAQTPYPIMINKWYIPQEISELTLTRIRQSDY"
     gene            2875..3582
                     /gene="deoD"
                     /locus_tag="POB55_001382"
     CDS             2875..3582
                     /gene="deoD"
                     /locus_tag="POB55_001382"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDI6500350.1"
                     /translation="MKSTPHIKPMNDVEIAETVLLPGDPLRAKFIAETYLDDVEQFNT
                     VRNMFGFTGTYKGKKVSVMGSGMGMPSIGIYSYELIHTFGCKKLIRVGSCGAMQENID
                     LYDVIIAQGASTDSNYVQQYQLPGHFAPIASYQLLEKAVGTARDKGVRHHVGNVLSSD
                     IFYNADTTASERWMRMGILGVEMESAALYMNAIYAGVEALGVFTVSDHLIHETSTTPE
                     ERERAFTDMIEIALSLV"
     gene            3589..4941
                     /gene="tet(38)"
                     /locus_tag="POB55_001383"
     CDS             3589..4941
                     /gene="tet(38)"
                     /locus_tag="POB55_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(38)"
                     /protein_id="HDI6500351.1"
                     /translation="MNVEYSKIKKAVPILLFLFVFSLVIDNSFKLISVAIADDLNISV
                     TTVSWQATLAGLVIGIGAVVYASLSDAISIRTLFIYGVILIIIGSIIGYIFQHQFALL
                     LVGRIIQTAGLAAAETLYVIYVAKYLSKEDQKTYLGLSTSSYSLSLVIGTLSGGFIST
                     YLHWTNMFLIALIVVFTLPFLFKLLPKENNTNKAHLDFVGLILVATIATTVMLFITNF
                     NWLYMIGALIAIIVFALYIKNAQRPLVNKSFFQNKRYASFLFIVFVMYAIQLGYIFTF
                     PFIMEQIYHLQLDTTSLLLVPGYIVAVIVGALSGKIGEYLNSKQAIITAIILIALSLI
                     LPAFAVGNHISIFVISMIFFAGSFALMYAPLLNEAIKTIDLNMTGVAIGFYNLIINVA
                     VSVGIAIAAALIDFKALNFPGNDALSSHFGIILIILGLMSIVGLVLFVSLNRWTQSEK
                     "
     gene            5022..5684
                     /gene="deoC"
                     /locus_tag="POB55_001384"
     CDS             5022..5684
                     /gene="deoC"
                     /locus_tag="POB55_001384"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDI6500352.1"
                     /translation="MKFEKYIDHTLLKPESTRTQIDQIIDEAKAYNFKSVCVNPTHVK
                     YAAERLADSEVLVCTVIGFPLGASTTATKAFETEDAIQNGADEIDMVINIGALKDGRF
                     DDVQQDIEAVVKVAKGHTVKVIIETVLLDHDEIVKASELTKAAGADFVKTSTGFAGGG
                     ATAEDVKLMKDTVGADVEVKASGGVRNLEDFNKMVEAGATRIGASAGVQIMQGLEADS
                     DY"
     gene            5712..6890
                     /gene="deoB"
                     /locus_tag="POB55_001385"
     CDS             5712..6890
                     /gene="deoB"
                     /locus_tag="POB55_001385"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDI6500353.1"
                     /translation="MTRPFNRVHLIVMDSVGIGEAPDAADFKDEGSHTLRHTLEGFDQ
                     TLPNLEKLGLGNIDKLPVVNAVEQPEAYYTKLSEASVGKDTMTGHWEIMGLNIMQPFK
                     VYPNGFPEELIQQIEEMTGRKVVANKPASGTQIIDEWGEHQMKTGDLIVYTSADPVLQ
                     IAAHEDIIPLEELYDICEKVRELTKDPKYLIGRIIARPYVGEPGNFTRTSNRHDYALK
                     PFGKTVLDHLKDGGYDVIAIGKINDIYDGEGVTEAVRTKSNMDGMDQLMKIVKKDFTG
                     ISFLNLVDFDALYGHRRDKPGYAQAIKDFDDRLPELFSNLKEDDLVIITADHGNDPTA
                     PGTDHTREYIPVIMYSPKFKGGHALESDTTFSSIGATIADNFNVTLPEFGKSYLKELK
                     "
     gene            complement(7021..7836)
                     /gene="phnE"
                     /locus_tag="POB55_001386"
     CDS             complement(7021..7836)
                     /gene="phnE"
                     /locus_tag="POB55_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HDI6500354.1"
                     /translation="MTQEIAKYNVHTKAHKRKLIKRWLIAIVVLAIIIWAFAGVPSLE
                     LKSKSLEILKSIFSGLFHPDISYIYIPDGEDLLRGLLETFAIAVVGTFIAAIICIPLA
                     FLGANNMVKLRPVSGVSKFILSVIRVFPEIVMALIFIKAVGPGSFSGVLALGIHSVGM
                     LGKLLAEDIEGLDFSAIESLKASGANKIKTLVFAVIPQIMPAFLSLILYRFELNLRSA
                     SILGLIGAGGIGTPLIFAIQTRSWDRVGIILIGLVLMVAIVDLISGSIRKRIV"
     gene            complement(7833..8633)
                     /gene="phnE"
                     /locus_tag="POB55_001387"
     CDS             complement(7833..8633)
                     /gene="phnE"
                     /locus_tag="POB55_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HDI6500355.1"
                     /translation="MPLETPTKYDSLLKKKVSLKTSFTFMLIIVLIIWSFIYTGFNFG
                     DLMIGIPQIGDLFKQMIPPDFEYLQQITTPMLDTIRMAIVSTVLGSIVSIPIALLCAS
                     NIVHQKWISIPSRFILNIVRTIPDLLLAAIFVAVFGIGQIPGILALFILTICIIGKLL
                     YESLETIDPGPMEAMTAVGANKIKWIVFGVVPQAISSFMSYVLYAFEVNIRASAVLGL
                     VGAGGIGLFYDQTLGLFQYPKTATIILFTLVIVVVIDYISTKVRAHLA"
     gene            complement(8635..9408)
                     /gene="phnC"
                     /locus_tag="POB55_001388"
     CDS             complement(8635..9408)
                     /gene="phnC"
                     /locus_tag="POB55_001388"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HDI6500356.1"
                     /translation="MSQIEFKNVSKVYPNGHVGLKNINLNIEKGEFAVIVGLSGAGKS
                     TLLRSVNRLHDITSGEIFIQGKSITKAHGKALLEMRRNIGMIFQHFNLVKRSSVLRNV
                     LSGRVGYHPTWKMVLGLFPKEDKIKAMDALERVNILDKYNQRSDELSGGQQQRISIAR
                     ALCQESEIILADEPVASLDPLTTKQVMDDLRKINQELGITILINLHFVDLAKEYGTRI
                     IGLRDGEVVYDGPASEATDDVFSEIYGRTIKEDEKLGVN"
     gene            complement(9622..10578)
                     /locus_tag="POB55_001389"
     CDS             complement(9622..10578)
                     /locus_tag="POB55_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDI6500357.1"
                     /translation="MKKFKCLFVLMLAVIVFAAACGNSSSLDNQKNASNDSDSKSGGY
                     KPKELTVQFVPSQNAGTLEAKAKPLEKLLSKELGIPVKVSVSTNYNTIVEAMKSKKVD
                     VGFLPPTAYTLAHDQKAADLLLQAQRFGVKEDGSASKELVDSYKSEILVKKDSKIKSL
                     KDLKGKKIALQDVTSTAGYTFPLAMLKNEAGINATKDMKIVNVKGHDQAVISLLNGDV
                     DAAAVFNDARNTVKKDQPNVFKDTRILKLTQAIPNDTISVRPDMDKDFQEKLKKAFID
                     IAKSKEGHKIISEVYSHEGYTETKDSNFDIVREYEKLVKDMK"
     gene            10807..12351
                     /locus_tag="POB55_001390"
     CDS             10807..12351
                     /locus_tag="POB55_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDI6500358.1"
                     /translation="MKKIYKSLTVSAIVATVSLSALPQSLAITHESQPTKQQRTVLFD
                     RSHGQTAGAADWVSDGAFSDYADSIQKQGYDVKAIDGHSNITEASLKSSKIFVIPEAN
                     IPFKESEQAAIVNYVKQGGNVVFISDHYNADRNLNRIDSSEAMNGYRRGAYEDMSKGM
                     NAEEKSSTAMQGVKSSDWLSTNFGVRFRYNALGDLNTSNIVSSKESFGITEGVKSVSM
                     HAGSTLAITNPEKAKGIVYTPEQLPAKSKWSHAVDQGIYNGGGKAEGPYVAISKVGKG
                     KAAFIGDSSLVEDSSPKYVREDNGEKKKTYDGFKEQDNGKLLNNITDWMSKDSDGKSL
                     KASGLTLDTKTKLLDFERPERSTEPEKEPWSQPPSGYKWYDPTTFKAGSYGSEKGADP
                     QPNTPDDHTPPNQNEKVTFDIPQNVSVNEPFEMTIHLKGFEANQTLENLRVGIYKEGG
                     RQIGQFSSKDNDYNPPGYSTLPTVKADENGNVTIKVNAKVLESMEGSKIRLKLGDKTL
                     ITTDFK"
     gene            12402..13937
                     /locus_tag="POB55_001391"
     CDS             12402..13937
                     /locus_tag="POB55_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HDI6500359.1"
                     /translation="MSNIAFYVVSDVHGYIFPTDFTSRNQYQPMGLLLANHVIEQDRR
                     QYDQSFKIDNGDFLQGSPFCNYLIAHSGSSQPLVDFYNRMAFDFGTLGNHEFNYGLPY
                     LKDTLRRLNYPVLCANIYENDSTLTDNGVKYFQVGDQTVGVIGLTTQFIPHWEQPEHI
                     QSLTFHSAFEILQQYLPEMKRHADIIVVCYHGGFEKDLESGTPTEVLTGENEGYAMLE
                     AFSKDIDIFITGHQHRQIAERFKQTAVIQPGTRGTTVGRVVLSTDEYENLSVESCELL
                     PVIDDSTFTIDEDDQHLRKQLEDWLDYEITTLPYDMTINHAFEARVAPHPFTNFMNYA
                     LLEKSDADVACTALFDSASGFKQVVTMRDVINNYPFPNTFKVLAVSGAKLKEAIERSA
                     EYFDVKNDEVSVSADFLGPKPQHFNYDIYGGVSYTIHVGRPKGQRVSNMMIQGHAVDL
                     KQTYTICVNNYRAVGGGQYDMFIDAPVVKDIQVEGAQLLIDFLSNNNLMRIPQVVDFK
                     VEK"
     gene            14006..14168
                     /locus_tag="POB55_001392"
                     /pseudo
     CDS             14006..14168
                     /locus_tag="POB55_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc peptidase"
BASE COUNT         4775 a         2264 c         2610 g         4519 t
ORIGIN      
        1 atggcattta aattaccaaa tttaccatat gcatatgatg cattggaacc atatatagat
       61 caaagaacaa tggagtttca tcacgacaaa catcacaata cgtacgtgac gaaattaaac
      121 gcaacagttg aaggaacaga gttagagcat caatcattag cggatatgat tgctaactta
      181 gacaaggtac cggaagcgat gaggatgtca gtccgtaata atggcggtgg tcattttaac
      241 cattcattat tctgggaaat actatcacct aattctgaag aaaaaggtgg cgtaatagat
      301 gacatcaaag cgcagtgggg cactttagat gaatttaaaa atgaatttgc aaataaagca
      361 acaacattat ttggatcagg ttggacttgg ttagttgtta atgatggcaa attagaaatt
      421 gtgacaacgc caaaccaaga taatccatta acagaaggca aaacaccaat cttactattt
      481 gatgtttggg agcatgccta ctatctgaaa tatcaaaata aacgtccaga ctatatgact
      541 gcattttgga atattgttaa ctggaaaaaa gttgatgaat tataccaagc agcaaaataa
      601 tataacttaa tataattgag gtggagcatc tacaaggtgt tccacttttt tgtacttaat
      661 atctttactt atttgttttt gtgtaatggg atagcacgta aaagtggcaa aataataaga
      721 taaatttcaa ctcgaaatac cttattattg ttgactataa tgatgaccta aatgttatac
      781 acatcagaaa atactattaa ttaatcaaca tattgacatt aaataaattg acaaaataag
      841 taattattgt gaatctaaag tgaaattttt ataaaaaaat gtaatgattc aaaaattttg
      901 ttgcatttct tttgtaatcg tatgataatg taaatgtaat caaattgtaa tataagggga
      961 caagacaatg aaaaaattag caacagtagg ttctttaatt gtaacaagca ctttagtatt
     1021 ctcaagtatg ccttttcaaa atgcgcatgc cgacacaact tcaatgaatg tgtcgaataa
     1081 acaaagccaa aatgtacaaa atcatcgtcc ttatggcgga gtagtaccac aaggaatgac
     1141 gcaagcacaa tatactgaat tagagaaagc tttatcccaa ttaagcgctg gcagtaatat
     1201 gcaagactat aatatgaaat tgtatgatgc gacgcaaaat attgctgata aatacaatgt
     1261 gataattaca actaatgtag gggtatttaa accacatgct gttagagata tgaatggcca
     1321 tgcgttacct ttaacaaaag atggcaattt ttatcaaacg aatgtagatg caaatggtat
     1381 taatcatggt ggtagtgaaa tggtgcaaaa taaaacaggt catatgagtc aacaaggcca
     1441 tatgaatcag aacacacaca tgaaccaaca gccacacatg caacaaggtc atatgcaatc
     1501 atcaaaccat caaatgatga gtccaaaagc aaatatgcat tcatcaaatc atcaaatgaa
     1561 ccaaagtaac aaaaaagttt taccagctgc tggtgaaagt atgacatcaa gtattcttac
     1621 tgcaagtatt gccgcactac tattagtatc tgggttattc ttagcattta gacgacgttc
     1681 aacaaataaa taaacataat acgattaata atagaaaaat cgtgtgatta tctgagcgag
     1741 cctaggacat aaatcaatgt cctaggctcg ctaatgttat attggcagta gttgactgaa
     1801 tgaaattgcg cttgtaacaa gcttttccat ttcttaccaa ctccctaaat agtcatcaaa
     1861 aaatttctta tattttaata attttaataa tccgattgtc ttatacgtgt cagtgttaat
     1921 tcagagattt cctgtggaat ataccactta ttaatcataa ttggataagg tgtttgtgcg
     1981 tacaatgttt caataatcag ccaacaatgt gtatcaccat caaacacgtg actatgattt
     2041 ttgaagtggg gcgctttggt aatagacatt tttaaatctg attgatatgc attgctataa
     2101 atcgtttgct caacgaatgt cttcatgtcg tcttcgtttt gtgtattcac tttaaatgtg
     2161 tcaatgacat ttaacggtat aaaggtaaag caaaatgcat cagcttgctt agaatgattg
     2221 tccttttttt gataatagcg ttccattgca atgacggcag aaggatggtt tgcaaacaaa
     2281 tgatttgtat attcactttc taaatcaaca cgataattaa ttgatgacat agatacgcga
     2341 gctagcaata tttgatcaag tggatgctta aattgatcca tacttgaagc gtgttgggca
     2401 tttgtttgtg gaataacaaa gtgtcccttc cctcttgtac tctctacgat gccatcttcg
     2461 gctaacaatt ttatagcttg gcgcaaagtc atacgactga catcaaagcg cgcacaaagt
     2521 tccttttcag taggtaatgc atggccactc ggatattttc ctatttgaat ttctttatat
     2581 aacgtattat aaatcgttaa aaattttggt tgtgtttgcg tcacgtagac aacctccata
     2641 aagttactta atcactctca tcatacaata atttttactc gaattggaaa aattataaaa
     2701 attaaatata gataggcttt gaaaattagt tttatacaag gttagtagct gtaactgtaa
     2761 aatgttctta atattgtcaa aatgtaatgc ttgaaagcgc ttttaaaaaa tattattata
     2821 tacatggtta gacaaataga caaatcacta tacaaatatt gggaggaata ttttatgaaa
     2881 tcaacaccac acattaaacc aatgaatgac gtcgaaattg cagaaacggt tctattgcca
     2941 ggagatccgt taagagctaa gttcattgca gaaacttatt tggatgatgt ggaacagttc
     3001 aatacagtgc gaaacatgtt tggttttacc ggaacatata aaggtaaaaa agtttctgtc
     3061 atgggttcag gtatgggtat gccatctatt ggcatttact cttatgaatt aattcataca
     3121 tttggttgta aaaaattaat tcgcgttggt tcttgtggcg cgatgcaaga aaacattgat
     3181 ttatatgatg tgattattgc acaaggtgcc tctactgatt caaattacgt tcaacaatat
     3241 caattaccag gtcattttgc gccaattgct tcttatcaat tattagaaaa agcagttgga
     3301 acagcacgtg acaaaggtgt acgtcatcat gtaggtaatg tgttatcaag tgatattttc
     3361 tataacgcgg atacaacagc gagtgaacgt tggatgcgta tgggtatttt aggtgtagaa
     3421 atggaatcag ctgcattata catgaatgca atttacgctg gtgtcgaagc attaggtgtg
     3481 ttcacagtga gcgatcattt aattcatgaa acgtcaacaa cacctgagga aagggaacgt
     3541 gcatttacag atatgattga aattgcactg tcattggttt agataattat gaatgttgaa
     3601 tattctaaaa taaagaaagc agtacctatt ttattattct tatttgtatt cagtttggtt
     3661 atagacaact catttaaatt gatttctgta gccattgctg atgacttaaa catttctgta
     3721 acgacagtaa gttggcaagc gacattagcc ggtttagtaa ttggtattgg cgctgtagta
     3781 tacgcttcat tatctgatgc cattagtata cgcacactat ttatttatgg cgtgatatta
     3841 atcattatcg gatcaattat tggttacatt ttccaacatc aattcgcatt acttttagtt
     3901 ggacgtatta ttcaaactgc cggtttagcc gctgcagaga cattatatgt gatatatgtt
     3961 gcaaagtatc tttctaaaga ggaccagaag acttaccttg gcttaagtac gagcagttat
     4021 tccttgtcat tagttatcgg tacattatca ggtggattta tttctacgta tttacactgg
     4081 acaaatatgt ttttaattgc attaatcgta gtatttacgt tgccattcct atttaaatta
     4141 ttaccaaaag aaaataatac gaataaagct catttagatt ttgttggctt aattctagtg
     4201 gcaactattg ctacaacagt catgctgttt attacgaact ttaattggtt atatatgatt
     4261 ggtgccttaa ttgcgattat cgtttttgcg ctatatatta aaaatgcgca acgtccatta
     4321 gtaaataaat catttttcca aaataaacgt tatgcttcat ttttatttat agtatttgta
     4381 atgtatgcta tccaattggg ttatattttt acgttcccat tcataatgga gcaaatttat
     4441 catctgcaac tagacacaac atcactgtta ttagtaccgg gttatatagt agcagtcatc
     4501 gttggtgcac taagtggtaa aatcggcgaa tatctgaatt caaaacaagc gattatcaca
     4561 gcaattattt taatcgcact gagcttgatt ttacctgcat ttgcagtagg taatcacatt
     4621 tcaatcttcg tcatttctat gatattcttt gcaggtagct ttgctttaat gtatgcacct
     4681 ttacttaacg aagccattaa aacaatagat cttaatatga caggtgtggc tattggtttt
     4741 tataatttaa ttattaatgt ggcggtatct gtaggtattg cgattgctgc ggctctaatc
     4801 gattttaaag cattaaattt cccaggcaat gatgcattaa gttcacattt cggtattatt
     4861 ttaattattt taggtttaat gagtattgtc ggattagttt tattcgtcag cttaaatcgt
     4921 tggacacaat ctgaaaaata aatagatata aattcgcgag atatattcgt atttatagta
     4981 aaattaaata aagagattat ataacacgag gagtagtaag tatgaaattt gagaaatata
     5041 tagatcacac tttattgaag cctgagtcaa cacgtacgca aatcgatcaa atcatcgatg
     5101 aagcgaaagc atacaatttt aaatctgtat gtgtgaatcc aacacatgtt aaatatgcag
     5161 cagagcgact agctgattca gaggtgctcg tttgtacggt aataggattc ccattaggtg
     5221 cgtcgacaac tgcaacgaaa gcatttgaaa cagaagatgc aattcaaaat ggtgcagatg
     5281 aaattgacat ggtcatcaac atcggcgcat taaaagatgg acgttttgat gatgtacaac
     5341 aagacattga agcagtggtt aaagttgcga aaggtcacac agtaaaagtg attattgaga
     5401 cggtattgtt ggaccatgac gaaattgtaa aagcgagtga attaacaaaa gcggctggtg
     5461 cggacttcgt taaaacttca acaggttttg caggtggcgg tgcgactgca gaagacgtta
     5521 aattaatgaa agatacagta ggtgctgatg tagaagtaaa agcatcaggt ggcgtacgta
     5581 atttagaaga tttcaataaa atggttgaag caggtgcgac acgtattggt gcgagcgcag
     5641 gtgttcaaat tatgcaaggt ttagaagcag attcagatta ctaatatata taaattttgg
     5701 gagtgatagc tatgacaaga ccatttaatc gtgtacattt aatcgtaatg gattcagtag
     5761 gtattggtga agcgccagac gcagctgatt ttaaagatga aggttcacat actttaagac
     5821 ataccttaga aggtttcgat caaactttac caaaccttga aaagttaggt ctagggaaca
     5881 tcgataaatt accagtagta aatgcagttg aacaaccaga agcatactat actaaattga
     5941 gtgaagcttc agttggtaaa gatacaatga ctggtcactg ggaaattatg ggtttaaata
     6001 ttatgcaacc ttttaaagta taccctaatg gattccctga agagttaatt caacaaattg
     6061 aagaaatgac aggtcgtaaa gttgttgcta acaaaccggc atcgggtacg caaattatcg
     6121 atgagtgggg cgagcaccaa atgaaaactg gcgacttaat tgtttataca agtgcagacc
     6181 cagtattgca aattgctgca catgaagaca ttatcccatt agaagagtta tatgatattt
     6241 gtgaaaaggt tcgtgagttg acaaaagacc ctaaatattt aattggtcgt attatcgcac
     6301 gtccatatgt tggtgaacca ggaaacttta cacgtacatc taatcgacat gactatgcgt
     6361 taaaaccttt tggtaaaact gtcttagatc atttgaaaga cggtggttat gatgttattg
     6421 ccatcggtaa aattaatgac atttatgatg gtgaaggtgt aacagaagcg gttcgtacga
     6481 agagtaacat ggacggtatg gatcaattga tgaaaattgt taagaaagat ttcacaggta
     6541 ttagcttctt aaacttagta gactttgatg cattatacgg tcatcgtcgt gataaaccag
     6601 gttatgcaca agcaattaaa gatttcgatg atcgcttgcc agaactgttt agcaacttaa
     6661 aagaagacga tttagtaatt attacagcag accatggtaa tgacccgaca gcgccaggta
     6721 cggaccatac gagagaatat atcccagtaa ttatgtacag tccgaaattt aaaggtggtc
     6781 atgcactaga aagtgatact acattcagtt ctatcggtgc aactatagca gataatttca
     6841 acgtaacatt accagagttc ggtaaaagtt atttaaagga attgaaatag aataaattta
     6901 gatataataa aaacagcagt gaagttaact ataacaatag ttttcttcac tgctgttttt
     6961 attataatag agaaacgtaa gacggtagga cctcttattt aggagtatcc tgatttaatg
     7021 ttaaacaata cgttttcgga ttgaaccgga aattaaatct acaattgcga ccattagtac
     7081 taaaccgatt aatataatac ctacacggtc ccaagaacgt gtttgaatgg caaatatgag
     7141 tggtgtcccg ataccaccag ccccaattag ccccagtata gaagctgaac gtaagtttag
     7201 ttcaaagcga taaagtatga gagatagaaa ggcaggcata atttgtggta tgactgcaaa
     7261 tacgagtgtt ttaatcttat tcgcaccact ggcctttaat gattctatag cactgaaatc
     7321 tagaccttca atatcttcag ctaaaagttt cccaagcata cctacagaat ggatacctaa
     7381 agctaataca cctgaaaatg aacctgggcc aacagctttg ataaatataa gtgccattac
     7441 aatttctggg aagacacgta taacacttaa aataaatttg ctaacacctg aaaccgggcg
     7501 taactttacc atattatttg cacctagaaa tgctaatgga atacagataa ttgcggcgat
     7561 gaaagtacct acaacggcta tcgcaaaggt ttcaagtaaa ccacgtaata agtcttcgcc
     7621 atctggtata tagatatagc tgatgtcagg atggaataat ccgctgaata tggattttaa
     7681 gatttctaat gatttacttt taagttctaa acttggtaca cctgcaaatg cccagatgat
     7741 aatagctaag acaacaattg caataagcca tcttttaatc aattttcgtt tgtgtgcttt
     7801 tgtgtgaaca ttatattttg ctatttcctg tgtcatgcga gatgtgccct cactttcgta
     7861 ctgatgtaat caatgacgac gacaataact aaagtaaata aaataatcgt tgctgttttt
     7921 ggatattgaa ataaaccaag tgtttgatca taaaacaatc caataccgcc agcgccgact
     7981 aatccaagca cagctgaagc acgtatattt acttcaaatg catataatac gtatgacata
     8041 aatgacgata tggcttgtgg tacaacaccg aaaacaatcc attttatttt attagcgcca
     8101 acagccgtca ttgcttccat tggacctgga tctatcgttt ccaatgattc atataataat
     8161 tttccaataa tacagatagt taaaataaac agtgctaata tccctggaat ttgaccgatt
     8221 ccaaatacag ccacaaagat tgctgctaat agcaaatccg gtatagtacg aactatattt
     8281 aaaataaaac gcgagggtat tgaaatccac ttttgatgaa cgatattgct agcacataat
     8341 aacgcaattg gtattgaaac gatgctacct aatactgtac ttacgatagc cattcgaata
     8401 gtatctaaca ttggcgttgt aatttgttgt aaatactcga aatcaggtgg aatcatttgt
     8461 ttgaatagat cacctatttg aggtattcct atcattaaat ctccaaaatt aaaccccgta
     8521 taaatgaagc tccaaatgat aagcacaatg attaacatga aggtaaaact cgtttttaaa
     8581 gaaacctttt tctttaaaag ggagtcatac tttgtaggtg tttctaaagg catgttagtt
     8641 cactcctagc ttttcatctt ctttaattgt acgtccatat atttcactaa atacgtcatc
     8701 tgttgcttca gatgcaggac catcatagac aacctcacca tcacgtaaac caatgatgcg
     8761 tgtgccatat tcttttgcta agtcaacaaa atgtaagtta attaaaattg tgatgcctaa
     8821 ttcttggttg atttttctta aatcatccat aacctgtttc gtagttaatg ggtctaatga
     8881 agcaactggc tcatctgcaa gaataatttc agattcttgg catagtgcac gtgcaataga
     8941 tatacgttgt tgttgacctc ctgataattc atcagagcgt tgattatatt tatctaagat
     9001 gttgacccgt tctagtgcat ccattgcttt aattttgtct tcttttggga ataaacctaa
     9061 taccattttc caagtagggt gataacctac acgtccactt agtacatttc gtaatacact
     9121 tgaccgttta actaaattaa aatgttggaa aatcatacct atatttcggc gcatttctaa
     9181 tagtgcttta ccatgggctt tagtgattga tttaccttgg atgaaaattt cacctgacgt
     9241 gatatcatgc aaacgattta cagatcttaa taacgtggat ttcccagcac cagatagtcc
     9301 gacaatgact gcaaattcac ctttttcaat atttaagtta atatttttca agcctacatg
     9361 accgttagga tagactttac tgacgttttt aaattcgatt tgactcatgt ttgacacctt
     9421 tctttaataa agaaaagcca aggtaaactg aaatatcata gacattgggt ttctgtgtac
     9481 aaatggtttc aaatgtacag cgacaccttt ctatatctgt ttcagtttag cgcctagctt
     9541 ttcaatatta atctagaata tatctattga acgaaagctt ttaataccaa attcgctaat
     9601 gattcatttg ttaaataatg attatttcat atctttaact aatttttcgt actctcttac
     9661 aatgtcgaaa tttgaatctt tcgtttctgt gtatccttca tgtgaataaa cttcgctaat
     9721 aattttgtga ccttcttttg atttagcaat gtctataaaa gcttttttca atttttcttg
     9781 gaaatcttta tccatatctg gtcttacaga aattgtgtca ttcggaatag cttgtgttaa
     9841 ttttaaaatt cgtgtgtctt taaatacatt tggttggtct tttttcacag tattacgtgc
     9901 atcgttaaat acagccgcag catctacatc tccatttaat aatgagataa ctgcttggtc
     9961 atgaccttta acattcacaa ttttcatatc tttagttgca ttaatacctg cttcgttttt
    10021 taacatcgca agtgggaatg tatatccagc agttgatgtt acatcttgta aggcaatttt
    10081 cttacctttt aaatctttca agcttttaat ttttgagtct tttttaacaa gaatttctga
    10141 cttataacta tctacaagtt ctttacttgc tgaaccatct tcttttacac cgaaacgttg
    10201 tgcttgtaat aataaatcag ctgctttttg atcatgtgct aatgtgtatg ccgttggtgg
    10261 taagaaacca acatcaactt ttttagactt catagcttca acaattgtat tgtagttagt
    10321 tgatacagac actttaactg gaatccctaa ttctttagat agtaattttt ctaatggttt
    10381 tgctttagcc tctaatgttc cagcattttg cgaaggtaca aattgtactg ttaattcttt
    10441 aggtttgtat cctcctgatt tagaatccga atcattacta gcgttctttt gattatctaa
    10501 agaacttgaa tttccacatg ctgctgcaaa aacaatgact gctaacatta atacaaataa
    10561 acacttaaat tttttcattt gataactgtc cccttcacat tcttaatcgt gtaatatgta
    10621 tgcaatgaaa ttacacttat agcgtaccac atttaaatgc gaaaaagtat tttataaaaa
    10681 tgaattttaa taactttatt taagagtttg ataagatttt gtaaattttt cgttaaattg
    10741 cccatttcaa acatatattt atcctataat tcaagaagaa atttatttta aaaggagcct
    10801 cacaaaatga aaaaaatata taagtcatta actgtctctg caattgttgc aacggtatca
    10861 ttaagtgctt taccgcaatc tttagctata acgcatgaat cgcaacctac aaagcaacag
    10921 cgaacggtat tattcgatcg ttctcatggt caaacagctg gtgctgcaga ttgggttagt
    10981 gatggtgcat tttcagatta tgcggattca atacaaaaac aaggttatga cgttaaagct
    11041 attgatggtc attcgaacat aacagaagca agtttgaaaa gttctaaaat atttgtaatt
    11101 cctgaggcta atattccttt caaagaatca gaacaggcag caattgttaa ctatgtgaaa
    11161 caaggtggca atgttgtctt tatttcagat cattacaatg ctgaccgaaa tttaaatcgt
    11221 attgattcat cagaggcaat gaatggttat cgacgtggag catatgaaga tatgtcgaaa
    11281 ggtatgaatg cagaagaaaa aagttctact gcaatgcaag gtgtgaaaag ttcagattgg
    11341 ttatctacaa actttggcgt acgttttcga tataatgcac taggtgattt aaatacgagc
    11401 aatattgttt cttcaaaaga gagtttcggt attactgaag gtgtgaaatc tgtctctatg
    11461 catgccggat caacattagc aattactaat ccagagaaag caaaaggtat tgtgtataca
    11521 ccagaacaat tgccagcgaa aagtaaatgg tcacatgctg tagatcaagg tatttataat
    11581 gggggcggta aagcagaagg cccctatgta gcaatttcta aagttggaaa aggtaaagca
    11641 gcatttatcg gtgattcatc acttgtggaa gatagttcgc ccaaatatgt aagagaagat
    11701 aatggagaaa agaagaaaac atatgatggt tttaaagaac aagacaacgg taagctatta
    11761 aataatataa cggattggat gtctaaagat agtgatggga aatcacttaa ggcgagtgga
    11821 ctaacattag atacaaagac taagttgctt gattttgaac gaccagagcg ttcaactgag
    11881 cctgaaaaag agccatggtc acaaccgccg agtggttata aatggtatga tccaacaaca
    11941 tttaaagcag gtagttatgg cagcgaaaaa ggcgcagatc ctcagccaaa cacaccagat
    12001 gatcatacgc caccaaatca gaacgaaaaa gtaacatttg atatcccgca aaatgtttct
    12061 gtaaatgagc catttgaaat gacaatacat ttaaaaggat ttgaagcaaa tcaaacactt
    12121 gaaaatctta gagttggtat ttacaaagaa ggcggacgtc aaatcggaca attttcaagt
    12181 aaagataacg attataaccc accaggttac agtactttgc caacagttaa agcagatgaa
    12241 aacggaaatg tcacaattaa ggtcaatgct aaagtacttg aaagtatgga aggttcaaag
    12301 attcgtttaa aactcggtga caaaaccttg attacaacag acttcaaata aatatataat
    12361 agaaataaga aagatgtttg tgattgaagg agtgagtgag gatgtcaaac atagcatttt
    12421 atgtcgtgag tgacgtacat ggttatattt tcccaacaga ttttacgagt agaaatcaat
    12481 atcaacctat gggattgtta ctagcgaatc atgttataga acaagacaga aggcagtatg
    12541 accaaagttt taaaatagat aatggtgatt ttttgcaagg gtcaccattt tgtaattact
    12601 taatcgcgca tagcggcagt agccagcctt tagttgattt ttataatcga atggcattcg
    12661 actttggtac gcttggtaat catgaattta attatggatt gccatactta aaagacactt
    12721 tacgcagact caattatcca gttttgtgcg ctaatatata tgaaaatgat agtacattga
    12781 ctgataacgg tgtgaagtat tttcaggttg gagatcaaac tgttggtgtg ataggtttaa
    12841 cgacacaatt tattccccat tgggaacaac cagagcatat tcagtcactt acgtttcata
    12901 gtgcttttga aatacttcaa caatacttac ctgaaatgaa gcgacatgca gatatcattg
    12961 tggtttgtta ccatggtgga tttgaaaagg atttagaaag tggtacgccg accgaagtat
    13021 taacgggtga aaatgaggga tatgccatgt tagaagcgtt ttctaaagat atagatatct
    13081 ttattacggg tcaccaacat cgacaaattg ctgaaaggtt taagcaaacg gctgtgattc
    13141 aacctggtac gagaggtaca actgtaggca gagtagtctt gagtactgat gaatatgaaa
    13201 atctatccgt tgaatcatgt gaattacttc ctgttataga tgattccaca tttactattg
    13261 atgaagatga ccaacattta cgaaagcagt tagaggactg gttagattac gaaattacta
    13321 cattgccata tgatatgacg attaatcatg catttgaggc acgtgtggca ccgcatcctt
    13381 ttacaaattt tatgaattac gctttattag aaaaaagtga cgcagatgtt gcctgtacag
    13441 ctttgtttga ttctgctagt ggtttcaagc aagtcgtgac gatgcgagat gttattaaca
    13501 attacccatt tccaaataca tttaaagttt tagctgtaag tggtgccaaa cttaaagaag
    13561 ccattgaacg atcagcagaa tattttgacg tgaaaaatga tgaagttagt gtgagcgcag
    13621 acttccttgg acccaaacca caacacttta attatgatat atatggtggc gtaagttata
    13681 ccattcatgt tggaagacca aagggacaac gtgtgagcaa tatgatgatt caaggtcacg
    13741 cagttgattt aaagcagaca tatacaattt gtgtaaataa ttatcgtgca gttggcggtg
    13801 gtcagtatga tatgtttatc gacgcgccag ttgtgaaaga tattcaagtt gaaggcgcac
    13861 aattacttat tgatttttta tcaaataata atttgatgcg tatcccgcaa gttgttgatt
    13921 ttaaagttga aaagtgacgg atatatgaaa aacttacttg attttaatcg ggtaagtttt
    13981 tgtttaaagc gagaaagtat tatctttaga cgacgtgatt gaaatgtcac gtcctgtcaa
    14041 aatgaatagt ttattaaagt tgtattttga tagaaattta ctgacaccac aaaaattatt
    14101 gaattattta aaagttgatg aaacattttt gaatcatcta gcaggtatta atttaaaact
    14161 ctttaagg
//
LOCUS       DAMZKC010000065        13683 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.065, whole genome shotgun sequence.
ACCESSION   DAMZKC010000065 DAMZKC010000000
VERSION     DAMZKC010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13683
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.065"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..894
                     /locus_tag="POB55_001393"
     CDS             <1..894
                     /locus_tag="POB55_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDI6500360.1"
                     /translation="NLFSSGSAQPNISATDIQNFIIALPEKETIKKIEIYINYQLKII
                     SNIIDTTYQSIEELKKYKQSLITEAVTKGIDPNVEMKESGNDWIGSIPSNWSVRKIKH
                     DFNLKGRIGWQGLTSNEYQTVGPYLITGTDFKKGIIRWDSCVRISEERFEEAPDIHIK
                     ENDLLITKDGTIGKVALATNVPKKVSLNSGVLLIREKLKNTINKKFMYYNLLSNMFWN
                     WYNSNNQGASTIKHLYQGQFYNYSYAIPLLHEQQQIVQYLDDKVSTIDRLIEDKTKVI
                     KELENYKKSLIYEYVTGKKEV"
     gene            907..3936
                     /locus_tag="POB55_001394"
     CDS             907..3936
                     /locus_tag="POB55_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HDI6500361.1"
                     /translation="MIKNKEINFEQDIEASLVQYGGWESVRFQDTHYDANIALDPTVL
                     LEFVKQTQPKNWKRYVSIYRTNAEQKFLKRLNEEITNHGILHVLRKGIRDRGVSFKVV
                     YFQPASSLNTENIKNYEANRFQCVRQFQYSNKYRNTIDMVLMINGIPLVALELKNQYT
                     GQNVEHARLQFEQDRDPKELIFQFNSRFLVYFAVDHFNVLMTTKLAKGNTYFLPFDQG
                     SNGPGRVGGAGNPSNPSGYPTSYLWENVLTSYQLLDIFERFMNLEEKKKLLIFPRYHQ
                     LDVVKKLISDTQHHGPGKNYLIQHSAGSGKSNSIAWLAYHLASLHSNEDQPIYSSVIV
                     VTDRTVLDRQLQNTLMSFEHTVGQVETIGDNKTSQDLKTAINDQKKIIITTLQKFPVI
                     YDEIDNTAGRSFAILVDEAHSSQTGSSAQKLKHALADKEASLKEYQEFEESVEDQSLD
                     DQDQIVQTLLSQGQHDNLSFFAFTATPKEKTIEMFGTKQADGSYHPFHIYSMRQAIEE
                     GFILNVLQNYMTYNTSYKIAKSIEEDPDLPKTEAVRAIAKYQSLHPWVLRQKAEIMIE
                     QFRSVTQKAVQGRGKAMIVTASRLHAVRYMQEFKKYIKEKGYTDMDVLVAFSGTVEDD
                     GIEYTEPKMNLTHSGERIQENQLKEAFHSEDFNLLIVAEKYQTGFDEPLLHTMFVDKR
                     LRGVKAVQTLSRLNRTMPGKTDTFILDFVNTAEEIYEAFQPFYEATSLKEEININLIY
                     DTQSKLRKFHIYNDDDIQTVMKLNRQAKQKQNDQLLGRLSSVLRPVISRYSDLPEDVQ
                     YDFRVTLRNFNKWYNYIAQIDRTFDKELLEESIFTGFLLKFIPEKQRNYIDIEDKVKL
                     EYYKLQEDFKGDIALVNEDVENGQLAHPSEVNATVRPEEEYDSLNEIIHKINERFPND
                     FTQGDRVLVKNMRETLINNTDAKLVNMAQNNSEEMFANSLFQQQFQDFLIDQYNQGEA
                     AYSKMFSNNRDFYDTVYQLLAKDYYRWLRSQKADNFA"
     gene            complement(4314..4793)
                     /gene="rlmH"
                     /locus_tag="POB55_001395"
     CDS             complement(4314..4793)
                     /gene="rlmH"
                     /locus_tag="POB55_001395"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020363758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HDI6500362.1"
                     /translation="MKITILAVGKLKEKYWKQAIAEYEKRLGPYTKIDIIEVTDEKAP
                     ENMSDKEIEQVKEKEGQRILAKIKPQSTVITLEIQGKMLSSEGLAQELNQRMTQGQSD
                     FVFVIGGSNGLHKDVLQRSNYALSFSKMTFPHQMMRVVLIEQVYRAFKIMRGEAYHK"
     gene            complement(5161..7479)
                     /gene="adsA"
                     /locus_tag="POB55_001396"
     CDS             complement(5161..7479)
                     /gene="adsA"
                     /locus_tag="POB55_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored adenosine synthase AdsA"
                     /protein_id="HDI6500363.1"
                     /translation="MKALLLKTSVWLVLLFSVMGLWQVSNAAEQHTPMKAHAVTTIDK
                     ATTDRQQVPPTKEAAHHYGEEAATNVSASAQGTADDTNNKVTSNAPTNKPTTAVSTTG
                     NKRHDVDAQQASTQKPTHTATFKLSNAKTASLSPRMFAANVPQTTTHKILHTNDIHGR
                     LAEEKGRVIGMAKLKTVKEQEKPDLMLDAGDAFQGLPLSNQSKGEEMAKAMNAVGYDA
                     MAVGNHEFDFGYDQLKKLEGMLDFPMLSTNVYKDGKRAFKPSTIVTKNGIRYGIIGVT
                     TPETKTKTRPEGIKGVEFRDPLQSVTAEMMRIYKDVDTFVVISHLGIDPSTQETWRGD
                     YLVKQLSQNPQLKKRITVIDGHSHTVLQNGQIYNNDALVQTGTALANIGKITFNYRNG
                     EVSNIKPSLINVKDVENVTPNKALAEQINQADQTFRAQTAEVIIPNNTIDFKGERDDV
                     RTRETNLGNAIADAMEAYGVKNFSKKTDFAVTNGGGIRASIAKGKVTRYDLISVLPFG
                     NTIAQIDVKGSDVWTAFEHSLGAPTTQKDGKTVLTANGGLLHISDSIRVYYDMNKPSG
                     KRINAIQILNKETGKFESIDLKRVYHVTMNDFTASGGDGYSMFGGPREEGISLDQVLA
                     SYLKTANLAKYDMTEPQRMLLGKPVASEQPAKGQQGSKGSESGKDTQPIGKEKVMNPA
                     KQPATGKVVLLPAHRGTVSSGTEGSDRALEGTAVSSKSGKQLANMSAPKGSAHEKQLP
                     KTGTDQSSSPAAMFVLVTGIGLIATVRRRKAS"
     gene            complement(7707..8507)
                     /locus_tag="POB55_001397"
     CDS             complement(7707..8507)
                     /locus_tag="POB55_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDI6500364.1"
                     /translation="MSRLIRMSVLASGSTGNATFVENEKGSLLVDVGLTGKKMEELFS
                     QIDRNIQDLNGILVTHEHIDHIKGLGVLARKYQLPIYANEKTWQAIEKKDSRIPMDQK
                     FIFNPYETKSIAGFDVESFNVSHDAIDPQFYIFHNNYKKFTILTDTGYVSDRMKGMIR
                     GSDAFIFESNHDVDMLRMCRYPWKTKQRILGDMGHVSNEDAGHAMTDVITGNTKRIYL
                     SHLSQDNNMKDLARMSVGQVLNEHDIDTEKEVLLCDTDKAIPTPIYTI"
     gene            complement(8896..9684)
                     /locus_tag="POB55_001398"
     CDS             complement(8896..9684)
                     /locus_tag="POB55_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HDI6500365.1"
                     /translation="MNWKLTKTLFIFVFILVNIVLVSIYVNKVNRSHINEVESNNEVN
                     FQQEEIKVPASILNKSVKGIKLEQITGRSKDFSSKAKGDSDLTTSDGGKLLNANISQS
                     VKVSDNNLKDLKDYVNKRVFKGSEYQLSEISSGSVKYEQTYDNFPILNNSKAMLNFNI
                     EDNKATSYKQSMMDDIKPTDGADKKHQVIGVRKAIEALYYNRYLKKGDEVINARLGYY
                     SVVNETNVQLLQPNWEIKVKHDGKDKTNTYYVEATNNNPKIINH"
     gene            complement(9685..11019)
                     /locus_tag="POB55_001399"
     CDS             complement(9685..11019)
                     /locus_tag="POB55_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500366.1"
                     /translation="MNNKEHIKSVILALLVLMSVVLTYMVWNFSPDIANVDNTDSKKS
                     ETKPLTTPMTAKMDTTITPFQIIHSKNDHPEGTIATVSNVNKLTKPLKNKEVKSVEHV
                     RRDHNLMIPDLSSDFTLFDFTYDLPLSTYLGQVLNMNAKVPNHFNFNRLVIDYDADDN
                     IVLYAISKDRHDYVKLTTTTKNDHFLDALAAVKKDMQPYTDIITNKDTIDRTTHVFAP
                     SKPEKLKTYRMVFNTISVEKMNAILFDDSTIVRSSKSGVTTYNNNTGVANYNDKNEKY
                     HYKNLSEDEASSSKMEETIPGTFDFINGHGGFLNEDFRLFSTNNQSGELTYQRFLNGY
                     PTFNKEGSNQIQVTWGEKGVFDYRRSLLRTDVVLNSEDNKTLPKLESVRSSLANNSDI
                     NFEKVTNIAIGYEMQDNSDHNHIEVQINSELVPRWYVEYDGEWYVYNDGRLE"
     gene            complement(11012..12838)
                     /gene="walK"
                     /locus_tag="POB55_001400"
     CDS             complement(11012..12838)
                     /gene="walK"
                     /locus_tag="POB55_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HDI6500367.1"
                     /translation="MKWLKQLQSLHTKLVIVYVLLIIIGMQIIGLYFTNNLEKELLDN
                     FKKNITQYAKQLEISIEKVYDEKGSVNAQKDIQNLLSEYANRQEIGEIRFIDKDQIII
                     ATTKQSNRSLINQKANDSSVQKALSLGQSNDHLILKDYGGGKDRVWVYNIPVKVDKKV
                     IGNIYIESKINDVYNQLNNINQIFIVGTAISLLITVILGFFIARTITKPITDMRNQTV
                     EMSRGNYTQRVKIYGNDEIGELALAFNNLSKRVQEAQANTESEKRRLDSVITHMSDGI
                     IATDRRGRIRIVNDMALKMLGMAKEDIIGYYMLSVLSLEDEFKLEEIQENNDSFLLDL
                     NEEEGLIARVNFSTIVQETGFVTGYIAVLHDVTEQQQVERERREFVANVSHELRTPLT
                     SMNSYIEALEEGAWKDEELAPQFLSVTREETERMIRLVNDLLQLSKMDNESDQINKEI
                     IDFNMFINKIINRHEMSAKDTTFIRDIPKKTIFTEFDPDKMTQVFDNVITNAMKYSRG
                     DKRVEFHVKQNPLYNRMTIRIKDNGIGIPINKVDKIFDRFYRVDKARTRKMGGTGLGL
                     AISKEIVEAHNGRIWANSVEGQGTSIFITLPCEVIEDGDWDE"
     gene            complement(12851..13552)
                     /locus_tag="POB55_001401"
     CDS             complement(12851..13552)
                     /locus_tag="POB55_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDI6500368.1"
                     /translation="MARKVVVVDDEKPIADILEFNLKKEGYDVYCAYDGNDAVDLIYE
                     EEPDIVLLDIMLPGRDGMEVCREVRKKYEMPIIMLTAKDSEIDKVLGLELGADDYVTK
                     PFSTRELIARVKANLRRHYSQPAQDTGNVTNEITIKDIVIYPDAYSIKKRGEDIELTH
                     REFELFHYLSKHMGQVMTREHLLQTVWGYDYFGDVRTVDVTIRRLREKIEDDPSHPEY
                     IVTRRGVGYFLQQHE"
BASE COUNT         4225 a         2581 c         2146 g         4731 t
ORIGIN      
        1 aacttattct catcaggatc agctcaacct aacatatctg ctacagatat acaaaatttt
       61 atcattgcgt tacctgagaa agaaactatt aaaaaaattg aaatatacat taattaccaa
      121 ttaaaaatta tctcaaatat catagacaca acatatcaat ctatcgaaga acttaaaaaa
      181 tacaaacaat cactcatcac agaagcggtg acaaaaggca tagacccgaa tgtggaaatg
      241 aaagaaagtg gtaatgattg gattggtagt atcccttcaa attggagtgt tcgaaaaatt
      301 aaacatgatt tcaatttaaa agggcgtatt ggatggcaag gtttaacttc taacgaatat
      361 cagactgtag gaccctatct cataacggga actgatttca aaaaaggcat aatcaggtgg
      421 gattcttgcg ttagaattag tgaagaacgt tttgaagaag ctccagatat tcatattaaa
      481 gaaaatgatt tattaatcac taaagacggc acgattggaa aagtcgcttt agcaacaaat
      541 gttccaaaaa aagtttcatt aaattcaggt gtactactga ttagggaaaa attaaaaaat
      601 actattaata aaaaatttat gtattataat ttgttatcaa atatgttttg gaattggtac
      661 aattctaata atcaaggtgc tagcaccatt aaacatctat atcaaggtca attctataat
      721 tattcttatg cgatccctct attgcatgaa caacaacaaa tcgtccaata tctagatgat
      781 aaagtttcta ctatcgatag gctcattgaa gataaaacaa aagttattaa agagttagag
      841 aattataaga aatcattaat ttatgagtat gtcacaggta aaaaagaagt gtaaggagtg
      901 atatcagtga taaaaaataa agaaattaat tttgaacaag atatagaagc gagtctagtt
      961 cagtacgggg gatgggagtc agtaagattt caagatactc attacgatgc aaatatcgca
     1021 ctcgatccaa cagtattatt agaatttgta aaacaaactc aaccaaaaaa ttggaagcga
     1081 tacgtatcaa tctatcgtac aaatgcagaa cagaagtttt taaaacgact aaatgaagaa
     1141 atcacaaatc acggcatctt acatgtcctt cgtaaaggta tcagagatcg aggagtctct
     1201 tttaaagttg tttactttca acctgcatca tctttaaata cagaaaatat aaaaaattat
     1261 gaagctaatc gttttcaatg tgtgcgacaa tttcaatata gtaataagta tagaaacact
     1321 attgatatgg tgttaatgat taatgggatt cctcttgttg cattagaact taaaaatcaa
     1381 tatacaggcc aaaatgtgga acatgcgaga ttacaatttg aacaagatag agatccaaag
     1441 gaattaattt ttcaatttaa ctctcgattc ttagtctatt ttgcagtaga tcattttaat
     1501 gtgttaatga caacaaaact cgcaaaaggg aacacttact ttctgccatt cgatcaagga
     1561 tctaatggtc ctggtcgtgt tggaggtgca ggaaatccat ctaatccatc tggataccct
     1621 acttcttatt tatgggagaa tgtactcaca agctatcaat tattggatat ttttgaaagg
     1681 tttatgaatt tagaagaaaa gaaaaaacta ctaatttttc cacgttatca ccaactggat
     1741 gttgtgaaaa aattaatatc agatactcag caccatggtc ctggaaagaa ttatctcatt
     1801 caacattctg ctggttcggg taagtctaat agtattgctt ggttggcata ccatctcgct
     1861 tccttgcata gtaatgagga tcaaccaatc tattcatcgg tcattgttgt tacagaccgt
     1921 actgttttag atcgccaact tcaaaataca ttaatgagtt tcgagcatac tgttgggcaa
     1981 gtcgaaacta ttggagataa taaaacttct caagatttaa aaacagctat aaatgaccaa
     2041 aagaaaatta ttattactac attacagaag ttcccagtaa tttatgatga aatagataat
     2101 acagcaggta gaagttttgc gattttggtc gatgaagcgc attcttctca aacaggaagc
     2161 agtgcacaaa aattaaaaca tgcgttagca gacaaagaag cttctttaaa ggaataccaa
     2221 gaatttgaag aaagcgttga agatcaatcg ctagatgacc aagatcaaat tgttcaaaca
     2281 ttactgtccc aaggtcaaca cgacaactta agtttttttg cttttacagc tacaccgaaa
     2341 gagaaaacga ttgaaatgtt tggaacaaaa caagcagatg ggtcctatca tccatttcat
     2401 atttatagta tgagacaagc cattgaagaa ggatttattc taaacgtttt acaaaattat
     2461 atgacataca acacttccta taaaattgcg aaatcgattg aagaagaccc agatttgcca
     2521 aaaacagaag cagttcgagc tatcgctaaa tatcaatctc ttcatccttg ggtacttaga
     2581 caaaaagccg agattatgat tgaacaattt agatcagtga cacaaaaagc agtccaaggt
     2641 agaggaaagg caatgattgt gacagcttca cgactccacg ccgtacggta tatgcaagag
     2701 ttcaaaaagt acatcaaaga aaaaggatac acggatatgg atgtgttagt cgctttttca
     2761 ggtactgtag aagatgatgg aattgaatat acagagccga agatgaactt aacacattct
     2821 ggagaacgca tccaagaaaa tcaactgaaa gaagcatttc atagtgaaga ctttaattta
     2881 ttaattgtcg cagaaaaata ccaaacggga tttgacgaac ctttacttca tacaatgttt
     2941 gtcgataaga gattacgcgg agtaaaggca gtccaaacat tgtctcgcct caatcgtact
     3001 atgcctggaa aaacagatac ttttattctt gattttgtaa atacagcaga agaaatatat
     3061 gaagcattcc aaccttttta cgaagcaact tctctaaaag aagaaattaa tattaattta
     3121 atttatgata cacagagtaa actacgtaaa ttccatatat acaatgatga tgatattcaa
     3181 acagtaatga aattaaatcg tcaagcaaaa caaaagcaaa atgatcaatt acttggtcga
     3241 ttgagtagcg tgttacgccc cgttattagt agatacagtg acctaccaga agatgttcaa
     3301 tacgatttca gagttacact aaggaacttc aacaaatggt ataactatat cgctcaaatt
     3361 gatcgtactt ttgataaaga gttactagaa gaaagtattt tcactggttt cttgttaaaa
     3421 tttattcctg aaaaacaacg caactatatc gatattgaag acaaagtaaa gttagaatac
     3481 tataaattgc aggaagattt caaaggagat atagcactcg tcaatgaaga tgtagaaaat
     3541 gggcaattgg ctcatccttc agaagtgaat gcaactgtta gacctgaaga agaatacgat
     3601 tctttaaatg aaattataca caaaatcaat gaacgttttc caaatgattt tactcaaggc
     3661 gatagagtac tcgttaaaaa catgcgagaa acattgataa acaatacaga tgcgaaactt
     3721 gtaaatatgg ctcaaaacaa cagcgaagaa atgtttgcga atagtttatt ccaacaacaa
     3781 ttccaagact tcttgattga tcaatataat caaggagaag ctgcatactc taaaatgttt
     3841 agcaataata gagactttta tgacactgtc tatcaactac tagcaaaaga ttattataga
     3901 tggctacgat cacaaaaagc agataatttt gcttaaatta attattttta acctaactta
     3961 aagattactc gcagtcttta agttagtttt tgctaaaatt tttaatttag ctatggtctc
     4021 tcatcctaat ttcactttca tacgatattt ttttaatatt tagttattct aaagtttcta
     4081 aattcttata ggatttttaa aagtttatct ttacttgcat gaaaaagcaa atcacctaca
     4141 tgctctaatt gtaatctact cgataaatgc cccgcatctc catacatctc aataaatccc
     4201 tataattcca attattgcac taactatttc acttaccatt gttactcact tctgattttc
     4261 aaataaatta aaggattcta atatctaatt aaatttttca agaaaaaacc gcatcattta
     4321 tgataagctt ctccacgcat aatcttaaat gctctgtaca cttgttcaat taacacaacc
     4381 cgcatcattt gatgtggaaa tgtcatttta ctgaatgata gtgcgtagtt actacgttgt
     4441 aagacgtcct tgtgcaggcc gtttgatccg ccaatgacga atacaaagtc gctttgccct
     4501 tgggtcatgc gttggttcaa ttcttgggcc aatccttcgg aagatagcat ctttccttgt
     4561 atttctaatg taatgactgt ggattgtggt ttgattttgg ctagtattcg ttggccttct
     4621 ttttctttta cttgctcgat ttctttgtcg ctcatatttt ctggtgcttt ttcgtctgta
     4681 acttctatga tgtctatctt ggtgtatggg cctaaacgtt tttcatattc tgctatggct
     4741 tgcttccaat atttctcttt tagtttccct acagctaaaa tggtgatttt catgtcgttt
     4801 ggtcctccaa attgttatca actttccagt tatccacaag ttattaactt gttcacactt
     4861 ttccctctta ttataccaag tttttttgca gtttttgata tttttctgac attcatgccc
     4921 aattttcagt gtataacttg tgtatttgtg gataactctg tgggcattgt gggtatttct
     4981 ctttaaattg tacacaattc tggataacta tccccatttc tgtggataaa taccttgatg
     5041 tcttattact tatccccgtt tttatatccc atatttatca acggttatag cttttttcca
     5101 taaaaaaaca ctaccgtacc tcttatttaa gaaatacagt agtattgttt tcaatatatt
     5161 ttagctagct tttctacgtc gtacagtcgc gattaaacct atacctgtta ctaatacaaa
     5221 catcgctgct gggcttgaac tttgatcagt tccagttttt ggtaactgtt tctcatgtgc
     5281 gctaccttta ggcgctgaca tgttagccaa ttgtttccca ctcttacttg atacagcagt
     5341 tccttctaat gcgcgatcag aaccttctgt accgctacta acagttcctc tatgcgctgg
     5401 taacaacaca actttacctg tcgctggttg tttcgctgga ttcatcactt tctctttacc
     5461 aattggttgt gtatctttac cagactcact acctttgcta ccttgttgtc ctttagctgg
     5521 ttgttcactt gctactggtt tacctaataa catacgttgt ggttctgtca tatcatactt
     5581 agctaagtta gctgttttta aataacttgc tagtacttga tctaatgaaa taccttcttc
     5641 tctagggcca ccgaacatac tatatccgtc tccacctgat gctgtgaagt cattcatcgt
     5701 tacatgatat acacgtttta aatcaatact ttcaaactta cctgtctctt tatttaaaat
     5761 ttgaatagca ttaattcgtt tgccagacgg tttattcata tcatagtaaa cacgaattga
     5821 atcagagata tgtaataaac cgccattcgc tgttaacact gtcttaccgt ctttttgtgt
     5881 tgttggtgca cctaaactat gttcgaaagc cgtccagacg tctgaacctt ttacatcaat
     5941 ttgcgcaatc gtatttccaa atggtaatac tgagattaaa tcatagcgtg tcaccttacc
     6001 ttttgcgata gaggcacgaa tacctccacc atttgtcacg gcaaagtcag tctttttaga
     6061 gaaattctta acgccatatg cttccatagc atctgcaatc gcgtttccta aatttgtttc
     6121 acgcgttcta acgtcatctc tttctccttt gaaatcaatc gtattatttg gaataataac
     6181 ctctgctgtt tgtgctctaa atgtttgatc agcttgatta atttgttcag ctaatgcttt
     6241 gttcggtgtt acattttcaa cgtctttaac attaatcaat gacggcttaa tattcgatac
     6301 ttctccattg cgataattaa atgtaatctt accgatattc gcaagtgctg tacctgtttg
     6361 tactaatgca tcattgttat aaatttgacc attttgaagt acggtatgtg aatgaccgtc
     6421 aatcactgta atacgtttct tcaattgtgg attttgactt aattgtttca ctaagtaatc
     6481 gccacgccat gtttcttgtg ttgaaggatc aatacctaaa tgagatataa caacaaatgt
     6541 atctacatct ttataaatac gcatcatttc cgctgtcaca ctttgtaatg gatctctaaa
     6601 ttcaacacct ttaatgcctt caggtcttgt tttcgtcttt gtttctggtg tcgttacgcc
     6661 aataattcca taacgaatac catttttcgt tacaatcgtt gaaggcttaa acgcgcgttt
     6721 tccatcttta taaacgttag tacttagcat cgggaagtct aacataccct ctaacttttt
     6781 caactgatcg tatccaaagt caaattcatg gttaccgact gccatagcat cataacctac
     6841 tgcattcatt gctttagcca tttcttcacc tttagattga tttgaaagtg gtaaaccttg
     6901 gaaggcgtct cctgcgtcta acattaaatc aggcttttct tgttctttta ctgtttttaa
     6961 tttagccata ccgatgacac gccctttttc ttcggctagt cggccatgga tatcatttgt
     7021 atgtaatatt ttatgtgttg ttgtttgtgg tacattagca gcaaacattc gtggtgaaag
     7081 tgatgctgtt ttagcatttg ataatttgaa tgttgctgtg tgagttggtt tttgtgttga
     7141 ggcttgttgt gcatctacat cgtgtctttt gtttcctgtt gttgaaactg ctgtagttgg
     7201 tttgttagtt ggtgcgttgg atgttacttt gttgtttgta tcatcagctg ttccctgcgc
     7261 tgatgctgat acgttggttg ccgcttcttc accataatga tgagccgctt cctttgttgg
     7321 cggtacttgt tgcctatctg ttgttgcttt gtctatcgtt gttactgcat gtgctttcat
     7381 tggtgtatgc tgctcagccg cgttcgagac ttgccataat cccatcacac taaaaagcaa
     7441 aacgagccat acacttgttt taagtaataa agctttcatt tcgttccctc cattaactgt
     7501 catacaccaa tatattatca taatccttat attaaaatga ttgattttat gtaaattatt
     7561 tataaattac atttaatatc gaattatttt acacatttca atcaagccgg ttcaatctaa
     7621 caacagccat ttcccaccaa taacataaaa aagcacccga taaagtcgtc tcacagcagt
     7681 gccgaacttt atagggtgac tctcatttat attgtatata ttggcgttgg aatagcttta
     7741 tccgtatcac atagcaatac ttctttttcc gtatcaatat cgtgttcgtt caatacttga
     7801 ccaacactca tacgcgccaa atctttcata ttattatctt gtgataaatg tgataggtaa
     7861 atacgtttcg tgttgcctgt aatcacatcc gtcatcgcat ggcccgcatc ctcattagat
     7921 acatgtccca tatcgcctaa aatacgttgt ttcgtcttcc atggataacg acacattctc
     7981 aacatatcga cgtcatgatt actctcaaaa ataaacgcat cgctgccacg tatcatacct
     8041 ttcatacgat ccgacacata tcccgtatcc gttaaaatcg taaacttctt atagttatta
     8101 tggaaaatat aaaattgcgg atctatcgca tcatgtgaca cgttaaacga ttcaacatcg
     8161 aaacctgcaa tagatttcgt ttcataagga ttaaaaatga atttctgatc catagggatg
     8221 cgactatctt tcttttcaat agcttgccaa gtcttttcat tcgcataaat tggcaattga
     8281 tatttacgcg ctaaaacacc taaaccttta atatggtcaa tatgttcatg ggttactaaa
     8341 ataccattta aatcttgaat attacggtca atttgactaa acaattcttc cattttcttg
     8401 ccagtcaaac caacatcaac taatagacta cctttttcat tttctacaaa agtggcgtta
     8461 cctgtactac cacttgctaa tacactcatg cgtatcaagc ggctcatcct ttctatttaa
     8521 gcagtcaatc tctctatatg actgcaatat acatcagtgc aaatatacga ttttccctac
     8581 agaaactatc agtatattag ctgttttgta tacctttcct attatgtaac tttagttcat
     8641 cgaccggtct cttataatta aattttgata atcacgtgca atgaatgtcc taattattat
     8701 aacaatgatt agacaaatac taaagttttt aaaataacag ttacactaat tttacaatgc
     8761 ggtactactt aaaatatgaa taaatgcgca cgttggagac acagaaacgt cagccataat
     8821 aaaccgtacc agcaaaaagc tagcaccgct tctcacatat gatgagcttg caatgctagc
     8881 ttattacgat tcatattaat gattaataat tttagggtta ttatttgtcg cttcgacata
     8941 gtaagtattc gttttgtcct taccgtcatg cttcacttta atttcccagt ttggttgtaa
     9001 caattgaaca ttcgtttcat tcacgactga atagtaacca agtctagcat taatgacttc
     9061 atcacctttt ttcaagtaac gattataata taatgcctcg attgcttttc tcacaccaat
     9121 cacttgatgc ttcttatctg caccatctgt gggcttaatg tcatccatca ttgattgttt
     9181 ataactagtc gctttgttat cttctatatt aaagttcaac atcgctttac tattgttcaa
     9241 aatcgggaaa ttatcatacg tttgttcgta ttttacagaa ccagaactaa tttcacttaa
     9301 ttgatattct gaacctttga acacacgttt gttaacataa tctttcaaat cttttaagtt
     9361 attgtcactg acctttaccg attgactaat gttcgcattt aataattttc caccatctga
     9421 tgtggtcaaa tccgaatcgc ctttagcttt agaactaaag tcttttgatc gccctgtaat
     9481 ttgctctaat tttatacctt taactgattt attcaatata ctagccggta ctttaatttc
     9541 ttcttgctga aaattaactt cattgttact ctcaacttca ttaatgtgtg agcgattgac
     9601 tttattaaca taaatcgata ctaacacgat gttgacaaga ataaacacga aaatgaaaag
     9661 tgtctttgtc agtttccagt tcatttattc aagcctccca tcgttataaa cataccattc
     9721 gccatcatat tctacatacc aacgcggtac gagttcactg ttaatctgca cttcaatgtg
     9781 attatgatct gagttatcct gcatttcgta accgatagcg atgtttgtta ctttttcaaa
     9841 attaatatca ctattgttcg ctaagcttga acgtacagac tctaatttcg gcaacgtttt
     9901 attatcttca ctgtttaaaa caacgtcggt gcgtaataac gaacgacgat aatcaaagac
     9961 gcctttttca ccccaagtga cttgaatttg attagagcct tctttattaa acgttggata
    10021 accattaagg aaacgttgat atgttaactc gcctgactga ttattcgtac taaacaatct
    10081 gaagtcttcg tttaagaaac caccatgacc attaataaaa tcaaaggttc ctggaatcgt
    10141 ttcttccatt ttgctggaac tcgcttcatc ttcggacaag tttttataat gatatttttc
    10201 atttttatcg ttatagtttg cgacacctgt attattgttg taggttgtaa caccactctt
    10261 tgaactacga acgatggttg aatcgtcaaa tagtatagca ttcattttct caacactaat
    10321 cgtgttaaat accatgcgat atgtttttaa cttttcaggt ttacttggtg caaaaacatg
    10381 cgtcgtacga tcaatcgtat ctttgtttgt gatgatatct gtgtatggtt gcatatcttt
    10441 tttcactgct gctaatgcat ctaaaaaatg atcatttttc gttgtagttg ttaattttac
    10501 gtaatcgtgg cgatctttgc ttatagcata aagcacgata ttatcatcag catcataatc
    10561 tatgaccaaa cgattgaaat tgaaatgatt tggtactttc gcattcatgt tcagtacttg
    10621 gccaagatat gttgataacg gtaaatcata cgtaaaatcg aataatgtaa aatcactgct
    10681 caaatcagga atcatcaagt tatgatcccg acgaacatgt tccacggact tcacttcttt
    10741 atttttcaaa ggtttcgtca gtttattcac attagatacc gtcgcgattg taccttctgg
    10801 atgatcattt ttcgaatgaa taatctgaaa tggcgtaata gttgtatcca ttttggctgt
    10861 cataggtgtc gttaaaggtt tcgtttcact cttcttacta tctgtattgt cgacatttgc
    10921 aatatcagga gaaaagttcc ataccatata tgtcaatacg acactcatca agacgagtag
    10981 cgctaaaatg acagatttaa tatgttcctt attattcatc ccaatcaccg tcttcaatga
    11041 cttcacatgg aagtgtgata aagatagatg taccttgacc ttctacactg tttgcccaaa
    11101 tacgaccatt gtgcgcttcc acaatctctt tcgaaatggc tagtcctaat ccagtaccac
    11161 ccattttacg cgtacgcgcc ttatctacac gatagaatcg gtcgaatatc ttatcgactt
    11221 tattgatagg aataccgatg ccattatctt taatacgaat cgtcattcga ttataaagtg
    11281 gattttgttt cacgtggaac tcgacacgtt tatcgcctct agaatatttc atcgcatttg
    11341 taatgacatt atcaaatact tgcgtcattt tatcaggatc aaattctgtg aaaatcgtct
    11401 ttttcggaat atctcgaata aatgttgtat ctttcgcaga catttcatgt cgattaataa
    11461 ttttattaat gaacatgtta aagtcgataa tttctttgtt gatttgatca gactcattat
    11521 ccattttaga taactgtagc aagtcattga ccagtcgaat cattcgttct gtttcttcac
    11581 gggtaacaga taaaaattgt ggcgcaagtt cctcatcttt ccatgcacct tcttcaagtg
    11641 cttcaatgta actattcata gaagttaaag gtgtacgtaa ctcatgtgat acattggcaa
    11701 caaattcacg acgctcacgt tcaacttgtt gttgttcagt aacgtcatgt aacacagcga
    11761 tataaccagt tacaaatcct gtttcctgca caatcgtact aaagttaaca cgtgcgatta
    11821 gaccttcttc ttcatttaaa tctaataaga aactatcatt attctcttga atttcttcaa
    11881 gtttaaattc atcttcaaga cttaatacac ttaacatgta atatccaatg atgtcttctt
    11941 tcgccatacc aagcatcttg agtgccatat cattgacgat acgaatacgt ccacggcggt
    12001 ctgttgcaat aataccatca ctcatatggg tgataactga gtccagtcta cgtttctcac
    12061 tttcagtatt agcctgcgct tcttgtacac gtttagacaa gttattaaat gctaaagcta
    12121 attcgccaat ttcatcatta ccataaatct tcacacgttg cgtatagtta cctctggaca
    12181 tttcaaccgt ctggttacgc atatcggtga ttggtttggt aatcgttcgc gctataaaga
    12241 atcctaggat gactgtgatt aataatgaaa tagctgtacc aacaatgaat atttgattta
    12301 tattatttaa ttggttataa acgtcattaa tttttgattc gatataaata ttaccaatta
    12361 cctttttatc gactttaact gggatattat atacccagac acggtcctta ccaccgccat
    12421 aatcttttaa aattaaatga tcgtttgatt gtcctagtga tagtgctttt tggacagaac
    12481 tatcattcgc tttttgattg attagactac ggttagactg cttcgtcgtc gcaataataa
    12541 tttggtcttt atctataaaa cgaatttctc caatttcttg acggttggca tactcactta
    12601 ataaattttg aatatctttt tgtgcattta cggagccctt ttcgtcatat actttttcaa
    12661 tactaatttc taattgttta gcgtactgcg taatattctt cttaaaatta tcaagcagct
    12721 ctttttcaag attatttgta aaatacagcc cgataatttg cataccaatg ataatcagta
    12781 atacataaac aattacaagt ttagtatgaa gggattgtag ttgttttagc cacttcattc
    12841 gtttcgacct ctactcatgt tgttggagga aatatccaac gcctctacgc gtcacaatat
    12901 attcaggatg tgacggatca tcttcaatct tttcacgtaa acgacgaatc gttacatcga
    12961 ccgtacgtac atcgccaaag taatcatagc cccatactgt ttgtaataaa tgttcacgtg
    13021 tcattacttg tcccatatgt tttgataaat aatggaacaa ttcaaattca cgatgtgtta
    13081 attcaatatc ttcgccacgt tttttaatag aatatgcgtc tggataaatc acaatatctt
    13141 taattgtgat ttcattcgtt acatttccag tgtcttgtgc tggttgtgag taatgacgac
    13201 gtaagttcgc tttcacacgt gcgattaatt cacgcgtact aaacggtttc gttacatagt
    13261 catctgcacc tagttctaaa ccaagcactt tatcaatttc tgaatcttta gcagtaagca
    13321 ttataattgg catttcgtat tttttgcgca cttcacgaca tacttccata ccatcacgac
    13381 caggtaacat gatatctagt aatacgatgt ctggttcttc ttcataaatt aagtcgactg
    13441 catcattacc atcgtatgca cagtacacat cgtatccttc tttttttaag ttaaattcta
    13501 aaatatcagc aatcggtttt tcatcatcaa ctacaacaac ttttctagcc atttgcataa
    13561 acctcttttc ttaaatcatt aatattattt tatcattttt tgttctttat cgtttatgtt
    13621 tgtgtaaaaa atcacaggat gtcgcttact atttttgtta ttgtgcgcat tttccgtctt
    13681 ata
//
LOCUS       DAMZKC010000066        13483 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.066, whole genome shotgun sequence.
ACCESSION   DAMZKC010000066 DAMZKC010000000
VERSION     DAMZKC010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13483
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.066"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(155..658)
                     /locus_tag="POB55_001402"
     CDS             complement(155..658)
                     /locus_tag="POB55_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDI6500369.1"
                     /translation="MSKWIIVSDNHTESGVLYQIYEMHPDADVYLHLGDSEFAYDDTE
                     LSLFNRVKGNCDFYPEFENEAVAKYNDVKAFYTHGHLYQVNRTRDLLAEKGLELGCLF
                     AFYGHTHVAKYEYINGVHVINPGSISQSRSSMEETYAEVIIDDQTLHGTINFKNRHHE
                     TISHTTF"
     gene            complement(651..1238)
                     /locus_tag="POB55_001403"
     CDS             complement(651..1238)
                     /locus_tag="POB55_001403"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HDI6500370.1"
                     /translation="MKEIVIASNNQGKINDFKVIFPDYHVIGISELIPDFDVEETGST
                     FEENAILKSEAAAKALNKTVIADDSGLEVFALNGEPGIYSARYAGENKSDEANIEKLL
                     NKLGNTTDRRAQFVCVISMSGPDMETKVFKGTVSGEIADGKYGENGFGYDPIFYVPKL
                     DKTMAQLSKEQKGQISHRRNAINLLQAFLEGDKNV"
     gene            complement(1250..2050)
                     /gene="racE"
                     /locus_tag="POB55_001404"
     CDS             complement(1250..2050)
                     /gene="racE"
                     /locus_tag="POB55_001404"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDI6500371.1"
                     /translation="MNKPIGVIDSGVGGLTVAKEIMRQLPNETIYYLGDIGRCPYGPR
                     PGEQVKQYTVEIARKLMEFDIKMLVIACNTATAVALEYLQKTLSIPVIGVIEPGARTA
                     IMTTRNQNVLVLGTEGTIKSEAYRTHIKRINPHVEVHGVACPGFVPLVEQMRYSDPTI
                     TSIVIHQTLKRWRNSESDTVILGCTHYPLLYKPIYDYFGGKKTVISSGLETAREVSAL
                     LTFSNEHASYTEHPDHRFFATGDPTHITNIIKEWLNLSVNVERISVND"
     gene            complement(2286..3101)
                     /gene="sdhB"
                     /locus_tag="POB55_001405"
     CDS             complement(2286..3101)
                     /gene="sdhB"
                     /locus_tag="POB55_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HDI6500372.1"
                     /translation="MTEQSVKNTPQHETQSKPKQKTVKLIIKRQDTSDSKPYEETFEI
                     PYRENLNVIACLMEIRRNPVNIKGEKTTPVVWDMNCLEEVCGACSMVINGRARQSCSA
                     IVDQLEQPIRLEPMNTFPVIRDLQVDRSRMFDNLKRMKAWIPIDGTYDLGPGPRMPEK
                     KRQTAYELSKCMTCGVCLEVCPNVTENNKFVGAQAISQVRLFNLHPTGSMTKDERLNA
                     LMGTGGLQQCGNSQNCVNACPKGIPLTTSIAAMNRETTFHMFKSFFGSDHEVE"
     gene            complement(3101..4867)
                     /gene="sdhA"
                     /locus_tag="POB55_001406"
     CDS             complement(3101..4867)
                     /gene="sdhA"
                     /locus_tag="POB55_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HDI6500373.1"
                     /translation="MAEKHLIVVGGGLAGLMSTIKAAEKGAHVDLFSVVPVKRSHSVC
                     AQGGINGAVNTKGEGDSPWIHFDDTVYGGDFLANQPPVKAMTEAAPKIIHLLDRMGVM
                     FNRTNEGLLDFRRFGGTLHHRTAYAGATTGQQLLYALDEQVRAYEVDGLVTKYEGWEF
                     LGIVKGDDDSARGIVAQNMTTAEIETFGSDAVIMATGGPGIIFGKTTNSMINTGSAAS
                     IVYQQGAIYANGEFIQIHPTAIPGDDKLRLMSESARGEGGRIWTYKDGKPWYFLEEKY
                     PDYGNLVPRDIATREIFDVCINQKLGINGENMVYLDLSHKDPHELDVKLGGIIEIYEK
                     FTGDDPRKVPMKIFPAVHYSMGGLYVDYDQMTNIKGLFAAGECDFSQHGGNRLGANSL
                     LSAIYGGTVAGPNAIDYISNIDRSYTDMDESIFEKRKAEEQERFDKLLAMRGTENAYK
                     LHRELGEIMTANVTVVRENEKLLETDKKIVELMKRYEDIDMEDTQTWSNQAVFFTRQL
                     WNMLVLARVITIGAYNRNESRGAHYKPEFPERNDEEWLKTTMASFQGAFEKPQFTYDD
                     VDVSLIPPRKRDYTSKSKGGKK"
     gene            complement(4919..5533)
                     /locus_tag="POB55_001407"
     CDS             complement(4919..5533)
                     /locus_tag="POB55_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b558 subunit"
                     /protein_id="HDI6500374.1"
                     /translation="MAQSKNEFYLRRIHSLLGIIPIGAFLVVHLLVNHQATQGAEAFN
                     KASNFMESLPFLIIVEFLFIYIPLLYHGLFGIHIAFTAKENVGHYSIFRNWMFFFQRV
                     SGILTFIFIGIHLWQTRLQKAFYGKEVNYDLMHETLQHPGWAIFYIICIIAVVFHFAN
                     GLWSFLVTWGGLQSPKSQRVFTWVSLIVFLVISYIGVTAIIAFM"
     gene            complement(5857..7638)
                     /gene="uvrC"
                     /locus_tag="POB55_001408"
     CDS             complement(5857..7638)
                     /gene="uvrC"
                     /locus_tag="POB55_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HDI6500375.1"
                     /translation="MEDYKQRIKNKLNVVPMEPGCYLMKDRNDQVIYVGKAKKLRNRL
                     RSYFTGAHDAKTTRLVGEIRRFEFIVTSSETESLLLELNLIKQYQPRYNILLKDDKSY
                     PFIKITKEKYPRLLVTRTVKQGTGKYFGPYPNAYSAQETKKLLDRIYPYRKCDKMPDK
                     LCLYYHIGQCLGPCVYDVDLSKYAQMTKEITDFLNGEDKTILKSLEERMLTASESLDF
                     ERAKEYRDLIQHIQNLTNKQKIMSSDKTIRDVFGYSVDKGWMCIQVFFIRQGNMIKRD
                     TTMIPLQQTEEEEFYTFIGQFYSLNQHILPKEVHVPRNLDKEMIQSVVDTKIVQPARG
                     PKKDMVDLAAHNAKVSLNNKFELISRDESRTIKAIEELGTQMGIQTPIRIEAFDNSNI
                     QGVDPVSAMVTFIDGKPDKKNYRKYKIKTVKGPDDYKSMREVVRRRYSRVLNEGLPLP
                     DLIIVDGGKGHMNGVIDVLQNELGLDIPVAGLQKNDKHQTSELLYGASAEIVPLKKNS
                     QAFYLLHRIQDEVHRFAITFHRQTRQKTGLKSILDDIDGIGNKRKTLLLRSFGSIKKM
                     KEATLEDFKNIGIPENVAKNLHEQLHK"
     gene            7804..7899
                     /locus_tag="POB55_001409"
     CDS             7804..7899
                     /locus_tag="POB55_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500376.1"
                     /translation="MLDYHLTFIDLLIPPFSSRKQYDDLSFNFLY"
     gene            complement(7962..8276)
                     /gene="trxA"
                     /locus_tag="POB55_001410"
     CDS             complement(7962..8276)
                     /gene="trxA"
                     /locus_tag="POB55_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDI6500377.1"
                     /translation="MAIVKVTDADFDSKVESGVQLVDFWATWCGPCKMIAPVLEELAA
                     DYEGKADILKLDVDENPSTAAKYEVMSIPTLIVFKDGQPVDKVVGFQPKENLAEVLDK
                     HL"
     gene            complement(8449..10797)
                     /locus_tag="POB55_001411"
     CDS             complement(8449..10797)
                     /locus_tag="POB55_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HDI6500378.1"
                     /translation="MRQKTLDVLEFEKIKSLVANETISDLGLEKVNQMMPATNFETVV
                     FQMEETDEIAQIYNKHRLPSLSGLSKVSAFIHRADIGGVLNVSELNLIKRLIQVQNQF
                     KTFYNQLVEEDEGVKYPILDDKMNQLPVLTDLFHQINETCDTYDLYDNASYELQGIRS
                     KISSTNQRIRQNLDRIVKSQANQKKLSDAIVTVRNERNVIPVKAEYRQDFNGIVHDQS
                     ASGQTLYIEPSSVVEMNNQISRLRHDEAIEKERILTQLTGYVAADKDALLVAEQVMGQ
                     LDFLIAKARYSRSVKGTKPIFKEDRTVYLPKAYHPLLNRETVVANTIEFMEDIETVII
                     TGPNTGGKTVTLKTLGLIIVMAQSGLLIPTLDGSQLSVFKNVYCDIGDEQSIEQSLST
                     FSSHMTNIVEILKHADKHSLVLFDELGAGTDPSEGAALAMSILDHVRKIGSLVMATTH
                     YPELKAYSYNREGVMNASVEFDVDTLSPTYKLLMGVPGRSNAFDISKKLGLSLNIINK
                     AKTMIGTDEKEINEMIESLERNYKRVETQRLELDRLVKEAEQVHDDLSKQYQQFQNYE
                     KSLIEEAKEKANQKIKAATKEADDIIKDLRQLREQKGADVKEHELIDKKKRLDDHYEA
                     KSIKQNVQKQKYDKIVAGDEVKVLSYGQKGEVLEIVNDEEAIVQMGIIKMKLPIEDLE
                     KKQKEKVKPTKMVTRQNRQTIKTELDLRGYRYEDALIELDQYLDQAVLSNYEQVYIIH
                     GKGTGALQKGVQQHLKKHKSVSDFRGGMPSEGGFGVTVATLK"
     gene            complement(10807..12519)
                     /gene="polX"
                     /locus_tag="POB55_001412"
     CDS             complement(10807..12519)
                     /gene="polX"
                     /locus_tag="POB55_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HDI6500379.1"
                     /translation="MTKKDVIKLLEQIATYMELKGENTFKISAYRKAAQSLELDERPL
                     DEISDVTELKGIGKGVAEVINDYRETGESQYLQQLQEEVPEGLIPLLKIQGLGSKKIA
                     KLYKELNIVDKASLQVACENGKVSELSGFAKKTEQNILEAVKQLGAKKDRYPIDQMRR
                     LNQEIIDYIDTLNYIDQYSSAGSFRRFKEMSKDLDFIISTDNPKAVQQQLLNIPNKVK
                     EVAVGNTKVSLELAYDDETIGVDFRLIEPSAFYHTLQHFTGSKEHNIRIRQLAKARDE
                     KVSEYGIEQADGTLIQYDSEAKIYEHFNVNFIPPAMREDGSEFDKDLSNIITIDDING
                     DIHMHTTYSDGAFSIRDMVEANIAKGYKFMVITDHSQSLRVANGLQVERLLRQNEEIK
                     ALDKEYSEIDIYSGTEMDILPDGSLDYDDEILAQLDYVIGAIHQSFNQSEEQIMERLA
                     NACRNPYVRHIAHPTGRIIGRRDGYKPNIEQLMALAEETNTVLEINANPHRLDLSADI
                     VRKYPNVKLTINTDAHHTNHLDFMNYGVATAQKGFVTKDRVINALSREAFKDFIENNT
                     KLKK"
     gene            complement(12592..13113)
                     /locus_tag="POB55_001413"
     CDS             complement(12592..13113)
                     /locus_tag="POB55_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HDI6500380.1"
                     /translation="MVIDFIIIIFFVYFVMVGFRRGFWLSMIHLSATIVSLWIASQFY
                     KSIVERLIVFIPYPKTTAFNTTFAFHFNHLQNRFEAIVAFLMITLFCKFILYLIIVTF
                     DKIIAYQNIHIFSRAMGMIVGVFMTIIVLHFTLYLLALYPNEALQHQLKISIVSHSLI
                     FHIPYLSAFTINL"
     gene            complement(13114..13380)
                     /gene="zapA"
                     /locus_tag="POB55_001414"
     CDS             complement(13114..13380)
                     /gene="zapA"
                     /locus_tag="POB55_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDI6500381.1"
                     /translation="MAQFKNKVNVSINDQLFTIVGEDNPEHIRYVAHLVDDKIKELGY
                     KAAGLDTSRKAILTAVNIMHEKVLLEEENRRLKQQIHKLQQREQ"
BASE COUNT         4093 a         2600 c         1884 g         4906 t
ORIGIN      
        1 attctccaaa attacaattc ttttcataac ataaatgtgt tattcattcc aattatctta
       61 taaaattgtc agaaatactt ttaaacaaac attcatgaca cgacctccta gttaaatcag
      121 tctataaact aaaaaaagcc atagctactt ctatttaaaa agtagtatga ctgattgttt
      181 cgtgatgtcg atttttgaaa ttgatggtgc catgtaaagt ttgatcatca ataataactt
      241 cagcatatgt ttcttccatt gaacttctag attgagatat acttccagga ttaataacat
      301 gaacaccatt aatatactca tattttgcca catgtgtatg tccataaaat gcaaacaaac
      361 aacctaattc aagtcctttt tcagctaata aatctcttgt tcgattgact tgatataaat
      421 gtccatgagt ataaaatgct ttcacgtcat tatattttgc gaccgcttca ttttcaaatt
      481 ctggataaaa atcacaattg ccctttactc tattaaataa gctaagttcc gtatcatcat
      541 acgcgaattc tgaatctcct aaatgtaaat atacatctgc atctgggtgc atttcataaa
      601 tttgatataa aacgcctgat tcagtatggt tatcactcac aataatccat ttagacattt
      661 ttatcacctt caagaaaagc ttgaagtaaa ttaatcgcat ttcgtctatg actaatttgc
      721 cctttttgtt cttttgaaag ttgagccatg gttttatcta atttcggtac ataaaatatc
      781 ggatcatatc cgaaaccatt ttcgccatat tttccatctg caatttcacc tgaaacagta
      841 cctttaaata cttttgtttc catatcaggg ccactcatac ttatgacaca aacaaattgc
      901 gcacgacgat cagttgtatt accaagctta tttaataatt tttcaatatt tgcttcatcg
      961 cttttatttt caccagcata acgtgcagag tatatacctg gctcaccatt taatgcaaaa
     1021 acttctagtc cactgtcatc agctatgact gttttattca atgcttttgc agcagcttct
     1081 gattttaata tagcattttc ttcaaatgtt gatcctgttt cttccacatc aaaatctggt
     1141 attagttctg aaatacctat tacgtggtaa tctggaaata ttactttaaa gtcatttatt
     1201 ttcccttgat tattcgatgc aataacaatc tctttcatta aaaatcctcc tagtcattca
     1261 ctgatatacg ttccacattg acagataaat ttaaccactc tttgataatg ttagtaatgt
     1321 gagtaggatc acctgttgca aaaaatcgat gatctggatg ttcagtataa cttgcatgtt
     1381 cattactaaa tgttagcaat gcactaactt cacgagccgt ttctaatcca gacgaaatca
     1441 ctgtcttttt accaccaaaa taatcataga taggtttata gagcaatgga tagtgggtac
     1501 atcctaaaat gacagtatca gactcactat tacgccaacg tttcagtgtt tgatgaatga
     1561 caatgcttgt aattgttgga tcactatatc tcatttgttc tacaagtggc acaaaacctg
     1621 gacaggcaac gccatgtacc tctacatgtg gattgatacg tttaatatgc gtacgatatg
     1681 cttcagattt aattgtgcct tccgttccta gtactaatac attttgattt ctagtagtca
     1741 ttattgctgt tctagcacct ggttcaatta cgccaatcac tgggattgat aaggtctttt
     1801 gtaaatattc taaagctaca gcagttgctg tattacaagc aatcacgagc atttttatat
     1861 caaattccat taatttacga gcgatttcaa ctgtatattg ttttacttgt tctcctggtc
     1921 ttggcccata tggacatcgt ccaatatcac ctaagtaata aatcgtctca tttggcaact
     1981 gacgcataat ttctttagct actgtcaaac ctccgacacc agagtctatt acacctattg
     2041 gtttattcat attcggtcat ccttactttt aatttaattt ttagtttatc ataactaagc
     2101 attggatttt agtattatgc actgtgttta ccatttttgt cattataata tttattttaa
     2161 atcagcccac tatcatattg tcatgtaatc tgcttattaa aaaaatccct tccaagttat
     2221 tgtgtatctc cattcaattt aattttgaaa ggaacataac attttaactc aaaagggatt
     2281 aatttttatt ctacttcatg gtctgaacca aagaatgatt taaacatgtg gaatgttgtt
     2341 tctctgttca ttgctgcaat ggatgttgtt aatggaatac ctttagggca agcattaaca
     2401 cagttttgtg aattaccaca ctgctgtaag ccaccagtac ccattaatgc atttaaacgt
     2461 tcatctttag tcatagatcc tgttgggtgc aaattaaaca aacgaacttg cgagattgct
     2521 tgcgcaccaa cgaatttatt attttcagta acattaggac aaacctctaa acatacacca
     2581 catgtcatac atttagataa ttcataagct gtttgacgtt ttttctctgg catacgtggt
     2641 cccggaccta aatcatacgt tccatcaatt gggatccatg ctttcatacg ttttaagtta
     2701 tcgaacattc tagaacgatc aacttgtaag tcacggataa ctgggaaagt attcattggc
     2761 tctaaacgaa taggttgttc taattgatca acaatcgcag aacaagattg tcttgcacga
     2821 ccattgataa ccatagaaca tgctccacat acttcttcta agcagttcat atcccagaca
     2881 acaggtgttg ttttttcacc tttaatatta actgggttac gtctaatttc cattaaacaa
     2941 gcaatgacgt ttaaattttc acgatatgga atttcaaatg tttcttcata aggcttagaa
     3001 tcacttgtat cttgtcgttt aataattaat tttactgttt tttgtttcgg tttagattgt
     3061 gtttcatgtt gtggagtgtt tttcactgat tgttcagtca ttatttttta ccccctttag
     3121 acttacttgt gtaatcacgt ttacgaggtg gtattaaact cacatcgacg tcatcataag
     3181 taaactgtgg tttttcaaat gcgccttgga atgaggccat tgtcgttttt aaccactctt
     3241 catcattacg ctctgggaat tctggtttat aatgggcacc gcgtgattcg ttacggttat
     3301 atgcaccaat cgtaataaca cgtgcaagta ctaacatgtt ccatagttga cgggtaaaga
     3361 ataccgcttg gttactccaa gtttgagtat cttccatatc aatatcttca taacgtttca
     3421 tcaattcaac aatcttttta tctgtttcta acagtttttc attttcacga acaacagtta
     3481 catttgctgt cataatttca ccaagttcac ggtgtaattt ataggcattt tctgtaccgc
     3541 gcatagctaa taatttatca aaacgttctt gctcttcagc tttacgtttt tcaaaaatac
     3601 tttcgtccat atcagtatat gatcgatcaa tatttgaaat ataatcaatc gcgtttggac
     3661 ctgctactgt accaccataa atcgctgata acaatgaatt ggcacctaag cggttaccac
     3721 catgttgaga gaagtcacat tctccagctg caaataaccc tttaatattt gtcatttgat
     3781 cataatctac atatagacca cccattgaat agtgaacagc tgggaaaatc ttcattggta
     3841 ctttgcgtgg gtcatcacca gtgaattttt cataaatctc aatgatacca cctagtttta
     3901 catctaactc atgtggatct ttatgtgaca aatcaagata taccatgttt tcgccattta
     3961 tacctaattt ttggttaata catacatcga aaatttcacg cgttgcgata tcacgaggta
     4021 ctaagttacc ataatcagga tatttctctt ctaagaagta ccaaggctta ccatctttat
     4081 atgtccaaat tcgtccacct tcaccacgtg ctgattcact cattagtcgc agtttatcat
     4141 caccagggat tgcagtagga tgaatttgaa tgaactcacc attagcataa atagcgcctt
     4201 gttggtaaac aatggaagcc gctgatcctg tattaatcat tgagtttgtt gttttaccga
     4261 aaataatacc agggccaccc gttgccataa taactgcatc tgaaccaaat gtttcaatct
     4321 cagcagttgt catattttgt gcaacgatac ctcttgcact atcatcgtca cctttaacta
     4381 tgccaaggaa ttcccatcct tcatacttcg taactaatcc atctacttca tatgcacgaa
     4441 cttgttcatc caatgcatat aataattgtt gtccagttgt tgcccctgca tatgctgttc
     4501 tgtgatgtaa tgtaccaccg aaacgtctaa aatctaatag accttcattt gttctattga
     4561 acattacgcc catacggtct aataaatgaa taattttagg tgctgcctct gtcatcgctt
     4621 taacaggtgg ttggtttgca aggaagtcgc caccatacac tgtatcatca aagtgaatcc
     4681 aaggagaatc gccttcccct ttagtattga ccgcaccatt aatgccacct tgggcacaaa
     4741 cagagtgcga acgctttact ggtacaactg agaacaaatc tacatgtgca cctttttctg
     4801 ccgctttaat tgttgacatt aagcccgcta ggccacctcc gacaacaata agatgtttct
     4861 ctgccataaa aatttcactc ccctaaattt tcaatctata tttgttaaat gcgatgtatt
     4921 acataaaggc aataattgca gtaacaccaa tatacgaaat aactaaaaat acgattaatg
     4981 aaacccatgt aaatactcgt tgtgattttg gagattgaag tccaccccaa gtaactaaga
     5041 atgaccataa gccatttgca aagtggaaca caacagcaat aatacaaata atataaaata
     5101 ttgcccatcc aggatgttgc aatgtttcgt gcattaaatc gtaattcact tctttgccgt
     5161 aaaatgcttt ttgtaaacgt gtttgccata aatggatacc aataaagata aatgttaaga
     5221 taccactcac tctttggaag aagaacatcc agtttctaaa aatcgagtaa tgtccaacat
     5281 tttcttttgc tgtaaatgca atgtgtatac caaacaaacc gtgatataac aacggaatgt
     5341 atataaataa aaattctaca ataattagaa atggtaatga ttccataaag ttagatgcct
     5401 tattaaacgc ttcagcacct tgtgttgctt ggtgattcac taataaatga acgaccaaaa
     5461 atgcacctat tgggataata cctaataacg agtgaatacg tcttagataa aattcatttt
     5521 ttgattgagc caaaaggagt cccccctgtg aacgaatatt taatttattg agctatttat
     5581 attaaacgta cgcttaaccc cctaaagtga tatcgtttct agcgttgtca ttatcattaa
     5641 gcgaaacatt ttaaagacaa atacacactg cacgatcacc aaactgcatg tcgaacaatg
     5701 taacatttgg attcgatatc taaaattgct tgtgatgata aactttctca tttagaaaac
     5761 gcttccacgt acattcaaaa aaataacttt gttaaccata ttgtaacatt atttcatata
     5821 ttttggggca tgagaatgat tctcacgccc agtaatttat ttatgcaatt gttcatgtag
     5881 gttctttgcg acgttttcag gaatacctat atttttaaaa tcttcaagtg tagcttcctt
     5941 cattttcttg attgaaccga atgaacgcaa taataatgtt ttacgtttgt taccgatacc
     6001 atctatatca tcaagtattg atttcaagcc tgtcttttga cgtgtttgtc tatgaaatgt
     6061 gattgcgaat ctgtgaacct catcttggat acggtgcaac aaataaaatg cctggctatt
     6121 tttcttcagt ggtacaattt ctgcactagc gccatataat aattcagatg tttggtgttt
     6181 atcatttttc tgcaaacctg caacagggat atcaagacct aattcgtttt gtagcacatc
     6241 aataaccccg ttcatatgtc ctttaccacc atctactatt attaaatcag gtaatggtaa
     6301 tccttcgttt aaaacgcgag aatatcgtcg tcttactact tctctcattg atttgtaatc
     6361 atctggacct ttaaccgttt tgattttata ctttctataa tttttcttat ctggtttacc
     6421 gtcgataaat gtaaccattg ctgacactgg atccacacct tgaatattag aattatcgaa
     6481 tgcttcaatt ctaattggtg tttgaattcc catttgtgtt ccaagttctt caatagcttt
     6541 aatcgttctt gactcatcac gtgatattaa ttcaaattta ttatttaagg atactttagc
     6601 gttatgtgca gctaggtcaa ccatatcttt tttgggacct cgcgcgggtt gaacgatttt
     6661 agtgtccaca acagattgaa tcatttcttt atccaaatta cgtggtacat gaacttcctt
     6721 aggtaaaata tgttggttta agctataaaa ttgtccaata aatgtataaa attcttcttc
     6781 ttctgtttgc tgtaatggaa tcatcgttgt atctcgcttt atcatattac cttgtcgtat
     6841 aaagaaaact tggatacaca tccatccttt atcaacacta taaccaaaga catcacgaat
     6901 cgttttatct gatgacataa ttttttgttt gtttgtcaga ttttgaatat gttgaattaa
     6961 atctctatat tctttagccc gttcaaaatc aagtgattca cttgcagtta acattcgctc
     7021 ttctaaactt tttaaaattg ttttgtcttc cccattcaga aaatcagtaa tttccttcgt
     7081 catttgtgcg tatttactca aatcaacgtc atatacacat ggtcctaaac attgtccaat
     7141 atggtaataa agacataatt tatctggcat cttatcacat ttgcgatatg gatatattct
     7201 gtctaataac tttttagttt cttgagcaga atatgcattc ggatacggtc cgaaatattt
     7261 gccagtacct tgttttacag ttctcgtcac tagtagtcta ggatatttct ccttcgtaat
     7321 tttaataaat ggataacttt tatcatcctt taataatata ttatatcttg gttgatattg
     7381 tttaatcaga ttcaattcaa gtaaaagtga ctctgtttca ctagacgtga caataaactc
     7441 aaagcgacgt atttcaccaa ccagtctcgt tgttttagca tcatgagcac ccgtaaaata
     7501 tgatctcaat cgatttctta gctttttagc tttgccaaca tatatcactt gatcattacg
     7561 atctttcatt aaatagcagc ctggttccat aggtacgaca tttaatttat ttttaattcg
     7621 ttgcttatag tcttccaaaa atatcccccc ttctaatttt aaaatgaatg atgctacaaa
     7681 agtgcttcaa atttatcacc actaattcta gttaataatc aacatcttcg agtatgactt
     7741 ttcatgcaca tcatgtaata ttgaacattg ttttatcaaa ccaactgcta caaatctatc
     7801 tgcatgctag attatcattt aacgttcata gatttattaa tcccaccatt ctcaagtcgt
     7861 aaacaatatg atgacttatc attcaacttt ttatattaaa aacacaaaca aaaactggag
     7921 caattcatta aagaaatgcc ccagtcgtca ttggttgtaa cttataaatg tttatctaaa
     7981 acttcagcta agttttcttt tggttggaaa ccaacaactt tatcaactgg ttgaccgtct
     8041 ttaaagacga ttaatgttgg aatactcatc acttcatatt tagctgcagt tgatggattt
     8101 tcatcaacat ctaattttaa aatgtcagct ttaccttcat agtcagctgc taattcttct
     8161 aataccggag cgatcatttt acatggacca caccatgttg cccaaaaatc tactagttgt
     8221 acaccagatt ctacttttga atcaaaatct gcatctgtta cttttacgat tgccataaat
     8281 gccaatcctc cttaattatt attatgatgt tcaaattata tcagataaca tgcttcatgt
     8341 atttctattt gctcaatttt tatgcttatt tcaaataaca tgtttgaaat gtattcttgt
     8401 tgctttacat tattttaact gcagctattt aatttatcaa attataattt attttagtgt
     8461 tgcaacggta acgccaaatc caccttcgct tggcatacca cctctaaagt cactaacact
     8521 tttatgcttt ttcaaatgtt gttgtacacc tttttgaagt gcacctgtac ctttaccatg
     8581 aatgatataa acttgttcgt aattacttaa aacggcttga tctaaatatt gatctagttc
     8641 aattaaagca tcctcataac gatagcctcg taagtcaagt tcagttttaa ttgtttgacg
     8701 attttgacgt gtaaccattt tcgttggctt aactttttct ttttgttttt tctctaaatc
     8761 ttcaataggt aacttcattt taataattcc catttgaaca attgcttctt catcattgac
     8821 aatttctaaa acttcaccct tttgaccgta agataatact tttacttcat caccagcaac
     8881 aattttatcg tatttttgct tttgtacatt ttgctttata gatttcgctt cataatgatc
     8941 atctaatcgt ttcttcttat caatcaattc atgttcttta acatctgcac ctttttgttc
     9001 acgcaattgt cttaagtctt taataatatc gtcagcttct tttgttgcag ccttaatctt
     9061 ctgatttgct ttttctttcg cttcctctat tagagacttt tcataatttt ggaattgttg
     9121 atactgctta gataaatcat catgcacttg ctccgcttct tttacaagac ggtccagttc
     9181 taacctctgt gtctctacac gtttgtaatt acgctctaat gattcaatca tttcatttat
     9241 ttctttttca tcagtaccaa tcatcgtctt agccttatta ataatattca aactaagacc
     9301 taacttttta gaaatgtcaa aagcatttga acgacccggc acacccatta ataacttata
     9361 cgttggactc aaagtatcta catcaaattc tacactcgca ttcataacgc cttctcgatt
     9421 ataactatat gctttaagtt caggatagtg cgtcgttgcc attactagag aaccaatttt
     9481 tctaacatga tctaaaatgc tcattgctaa tgcagcacct tcactcggat ctgtacctgc
     9541 acctaattca tcaaataaaa ctaaactatg tttgtctgca tgctttaaaa tttcaactat
     9601 attcgtcata tgagatgaaa aagttgataa tgattgttct attgattgtt catctccgat
     9661 atcgcaatat acatttttaa atacacttaa ctgactacca tcaagtgtgg gaatcaacaa
     9721 tcctgattga gccataacaa taattaaacc taatgttttt aatgttacag ttttaccacc
     9781 tgtattcggt cctgtaataa ttaccgtttc aatatcttcc ataaattcga tggtattagc
     9841 tacaacagtc tcacgattta ataatggatg gtatgcttta ggtaaatata cagtacggtc
     9901 ctctttaaat atcggctttg ttcctttaac acttctacta tatctcgctt ttgcgattaa
     9961 aaaatctaac tgacccatga cttgttctgc cacaagtagt gcatctttgt ccgcagccac
    10021 ataaccagtt agttgcgtta aaatgcgttc tttttcaatt gcttcgtcat gacgtaatcg
    10081 actaatttga ttattcattt caacaactga tgatggctca atatacaatg tttgtcctga
    10141 agcagattga tcatgtacaa tcccattaaa atcttgtcga tattcagctt tgacaggtat
    10201 aacgtttctt tcattcctaa ctgttacaat agcatctgat aattttttct gatttgcttg
    10261 gcttttaaca atacggtcca aattttgtct aatacgttga ttcgtgctag aaattttact
    10321 tctaatccct tgcaattcat aactcgcatt atcatataaa tcatacgtat cgcatgtttc
    10381 atttatttga tgaaaaagat cagttaacac aggtaattga ttcatcttgt catctaatat
    10441 tgggtattta acaccttcat cttcttcaac caattgatta taaaatgtct tgaattgatt
    10501 ttgtacttga attaatcttt ttatcaagtt aagctctgat acatttaaaa cgccgccaat
    10561 atcagcgcga tgaatgaatg ctgatacttt agataagcca ctcaagcttg gtaaacgatg
    10621 cttattataa atttgagcaa tctcatccgt ttcttccatt tgaaaaacaa ccgtttcaaa
    10681 attagtagct ggcatcattt gattgacctt ttccaagcct aagtcactaa tagtttcatt
    10741 ggcaacgagt gattttattt tttcaaattc taagacgtct aatgtttttt gtctcataaa
    10801 atccctctat ttcttaagtt ttgtattatt ttcaataaag tctttaaaag cttcacgcga
    10861 taatgcgtta atcactctat cttttgttac aaatcctttt tgcgcagttg ctacgccata
    10921 attcataaaa tctaaatgat ttgtatgatg cgcatcagtg ttaatagtta atttcacatt
    10981 tggatattta cgaacgatat cagcgctcag atccagtcga tgtggattgg cattaatttc
    11041 taatactgta ttcgtttctt cagctaatgc cattaattgt tcaatattcg gtttataacc
    11101 atctcttcta cctataatac gccctgttgg atgcgctata tgtcgcacgt atggattgcg
    11161 acatgcatta gctaatcgtt ccataatttg ttcttctgat tggttaaagc tttgatgaat
    11221 agctccaatt acataatcaa gttgtgctaa aatttcatca tcataatcca gcgagccatc
    11281 aggtaatata tccatttctg tacctgaata aatatcaatt tcactatatt ctttatctaa
    11341 agccttaatt tcttcgtttt gtcttaaaag tctttctact tgtaagccat tagcaacacg
    11401 taaactttgt gaatgatcag taattaccat gaatttataa ccttttgcga tatttgcttc
    11461 taccatgtct cgaatagaaa acgcaccatc actatacgtt gtatgcatat gaatatcacc
    11521 attaatatca tctattgtaa tgatattact tagatcttta tcaaattcgc taccatcttc
    11581 tcgcatagca ggtggtataa aattcacatt aaaatgttca tatatcttgg cttcactatc
    11641 atattgaatt aatgtaccat cagcttgttc aattccatat tcacttactt tttcatcacg
    11701 tgctttagca agttgtcgaa ttcttatatt atgttctttt gacccagtaa aatgctgcaa
    11761 tgtatgataa aaagcacttg gttcaattaa tcgaaaatcg acaccaatcg tttcatcatc
    11821 atacgctaat tctaatgaaa cttttgtgtt ccccactgca acttctttta ctttattggg
    11881 aatatttaat aattgctgct gcactgcttt tgggttatcg gtacttatta tgaaatctaa
    11941 atctttgctc atttctttaa aacgacggaa gcttcctgca gatgaatatt gatcgatata
    12001 atttaatgta tctatataat caatgatttc ttgattaagt cttctcattt gatcaattgg
    12061 atatctatct ttcttagcac caagttgttt cacagcttct aatatgtttt gttccgtttt
    12121 cttagcaaat ccgcttaatt cactaacttt tccattttca caagcaactt gaagtgacgc
    12181 tttatcaaca atattcaact ctttatatag cttagcaatt ttcttgcttc caagtccttg
    12241 aattttcaaa agtggaataa gaccttccgg aacttcttcc tgtaattgct gtaaatactg
    12301 agattcaccg gtctcacggt aatcattgat tacttctgca acacctttac caatgccttt
    12361 taactccgtt acatcagata tttcatctaa tggtcgttca tctaattcaa gactttgagc
    12421 tgcttttcga tacgctgata ttttaaaagt attttcccct tttaattcca tataagtagc
    12481 aatttgttct aatagtttga taacatcttt ttttgtcata ataacactcc ataaaaagaa
    12541 gaccaggacg tatcattaat atataccttt gtcctgacct cttatgataa tttataaatt
    12601 aatggtgaaa gccgataaat atgggatgtg aaaaatcaat gaatgactca caatagatat
    12661 tttaagctga tgttgtaatg cttcgttagg atataatgcc aatagatata acgtaaagtg
    12721 taagacaatt atcgtcataa acacaccaac tatcattccc attgcacgac tgaaaatatg
    12781 aatgttttga tacgctatta ttttatcaaa agttacgata attagatata aaatgaactt
    12841 acaaaacaat gtaatcatta aaaaagctac aatcgcttca aatcgatttt gtagatgatt
    12901 aaaatgaaac gcaaaagttg tattaaatgc tgttgtttta ggatatggaa taaatacaat
    12961 taatctttct acaatagatt tgtaaaattg actggcaatc cacaatgata caatcgttgc
    13021 actcaaatgt atcatagata accaaaaacc tcgtctgaat ccaaccatga caaaatacac
    13081 aaagaaaatg attatgataa aatcaatgac catttattgc tcacgctgct gcaatttgtg
    13141 aatttgttgt ttcaaacgtc gattttcttc ttctagtagt actttttcat gcataatatt
    13201 cacagcagtt agtattgctt ttcttgaagt atctaaacct gctgctttat accctaattc
    13261 ttttatttta tcatcaacta aatgtgctac atatcgtatg tgctctgggt tatcttcccc
    13321 aacaattgta aaaagctgat cattaattga tacatttacc ttgtttttaa actgtgccat
    13381 ttataatttc tcctgatcct ttttttaaaa tctaaattca cgttataaaa tatgactgga
    13441 tagtttgtct gaatttgata ctaatattgt tatattgcaa tta
//
LOCUS       DAMZKC010000067        13159 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.067, whole genome shotgun sequence.
ACCESSION   DAMZKC010000067 DAMZKC010000000
VERSION     DAMZKC010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13159
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.067"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            23..994
                     /locus_tag="POB55_001415"
     CDS             23..994
                     /locus_tag="POB55_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6500382.1"
                     /translation="MKYLLKGNILLLLLILLTIISLFIGVSELSIKDLLHLTESQRNI
                     LFSSRIPRTMSILIAGSSLALAGLIMQQMMQNKFVSPTTAGTMEWAKLGILIALLFFP
                     TGHILLKLVFAVICSICGTFLFVKIIDFIKVKDVIFVPLLGIMMGGIVASFTTFISLR
                     TNAVQSIGNWLNGNFAIITSGRYEILYLSIPLLALTYLFANHFTIVGMGKDFTNNLGL
                     SYEKLINIALFITATITALVVVTVGTLPFLGLVIPNIISIYRGDHLKNAIPHTMMLGA
                     IFVLFSDIVGRIVVYPYEINIGLTIGVFGTIIFLILLMKGRKNYAQQ"
     gene            981..1937
                     /locus_tag="POB55_001416"
     CDS             981..1937
                     /locus_tag="POB55_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDI6500383.1"
                     /translation="MRNNNKKIMLLIAVTLLISMLYLFVGIDFEIFEYQFSSRLRKFI
                     LIILVGAAIATSVVIFQAITNNRLLTPSIMGLDAVYLFIKVLPVFLFGIQSVWVTNVY
                     LNFILTLITMVLFALILFQGIFKIGHFSIYFILLIGVLLGTFFRSITGFIQLIMDPES
                     FLAIQSSMFANFNASNSNLVIFSAVLLVILLVITILLLPYLDVLLLGRAEAINLGISY
                     EKLTRILLVIVSVLVSVSTALVGPITFLGLLTVNLAHELMKTYEHKYILIATICLSWI
                     SLFSAQWVVENVFEATTEMSILIDLIGGSYFIYLLVRRRNAQ"
     gene            1934..2695
                     /locus_tag="POB55_001417"
     CDS             1934..2695
                     /locus_tag="POB55_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6500384.1"
                     /translation="MIQVENLTKTINNQMILEDISIDIEKGKLTSLIGPNGAGKSTLL
                     SAICRLIRFDNGEVKIDGQLMSDYKNNDLSKKISILKQTNHTEMNITVEQLVNFGRFP
                     YSKGRLTKEDHDIVNDALDLLQLQDIRNRNIKSLSGGQRQRAYIAMTIAQDTEYILLD
                     EPLNNLDMKHAVQIMQTLKMLAHKMNKAIVIVLHDINFASCYSDQIVALKNGQLVKSD
                     LKDNVIQSSVLSDLYDMNIQIEHIRNQRICLYFKD"
     gene            2814..3842
                     /locus_tag="POB55_001418"
     CDS             2814..3842
                     /locus_tag="POB55_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HDI6500385.1"
                     /translation="MKKTVLYLVLAVMFLLAACGNNSDKEQSKSETKGSKDTVKIENN
                     YKMRGEKKDGSDAKKVKETVEVPKNPKNAVVLDYGALDVMKEMGLSDKVKALPKGEGG
                     KSLPNFLESFKDDKYTNVGNLKEVNFDKIAATKPEVIFISGRTANQKNLDEFKKAAPK
                     AKIVYVGADEKNLIGSMKQNTENIGKIYDKEDKAKELNKDLDNKIASMKDKTKNFNKT
                     VMYLLVNEGELSTFGPKGRFGGLVYDTLGFNAVDKKVSNSNHGQNVSNEYVNKENPDV
                     ILAMDRGQAISGKSTAKQALNNPVLKNVKAIKEDKVYNLDPKLWYFAAGSTTTTIKQI
                     EELDKVVK"
     gene            complement(4159..4473)
                     /locus_tag="POB55_001419"
     CDS             complement(4159..4473)
                     /locus_tag="POB55_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500386.1"
                     /translation="MPKVYGSLIDTETRCRHYFTEEDIIAIKFKCCNKYYPCYKCHNE
                     FEKHAIKRWSEPSFNEKAILCGVCKHELTINEYMMVERCPNCQSRFNNRCKYHYHIYF
                     EI"
     gene            complement(4491..5414)
                     /locus_tag="POB55_001420"
     CDS             complement(4491..5414)
                     /locus_tag="POB55_001420"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HDI6500387.1"
                     /translation="MINKDIYQALQQLIPNEKIKVDEPLKRYTYTKTGGNADFYITPT
                     KNEEVQAVVKYAYQNEIPVTYLGNGSNIIIREGGIRGIVISLLSLDHIEVSDDAIIAG
                     SGAAIIDVSRVARDYALTGLEFACGIPGSIGGAVYMNAGAYGGEVKDCIDYALCVNEQ
                     GSLIKLTTKELELDYRNSIIQKEHLVVLEAAFTLAPGKTTEIQAKMDDLTERRESKQP
                     LEYPSCGSVFQRPPGHFAGKLIQDSNLQGHRIGGVEVSTKHAGFMVNVDNGTATDYEN
                     LIHYVQKTVKEKFGIELNREVRIIGEHPKES"
     gene            complement(5541..6059)
                     /locus_tag="POB55_001421"
     CDS             complement(5541..6059)
                     /locus_tag="POB55_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HDI6500388.1"
                     /translation="MYSNVQPFINSDLTNCFQSQYTEIESLLFNLLDSPVKYTKHIGG
                     THHFKYSTEPILDILVGVENLHDITSLDEKRLNYVGFYRLHHKYNKKVMMAKFNNFHE
                     LKQQVRLHIIQMNTQTFESYLALDYLLSTNDDIALQFHTKKHQILQHSLTIRQYEMQK
                     QQYFEKLCKKYL"
     gene            6179..7057
                     /locus_tag="POB55_001422"
     CDS             6179..7057
                     /locus_tag="POB55_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EMYY motif lipoprotein"
                     /protein_id="HDI6500389.1"
                     /translation="MKKIVIIAVLAILFVVISACGNKEKEAQHQFTKQFKDVEQKQKE
                     LQHVMDNIHLKEIDHLSKTDTTDKNSKEFKALQEDVKNHLIPKFEAYYKSAKNLPDDT
                     MKVKKLKKEYMTLANEKKDAIYQLKKFIGLCNQSIKYNEDILDYTKQFEKNRYKVESE
                     IKLADNKSEATNLTTKLEHNNKALRDTAKKNLDDSKENEVKGAIKNHIMPMIEKQITD
                     INQTNISDKHVNNARKNAIEMYYSLQNYYNTRIETIKVSEKLSKVDVDKLPKKGIDIT
                     HGDKAFEKKLEKLEEK"
     gene            7211..7531
                     /gene="ytxJ"
                     /locus_tag="POB55_001423"
     CDS             7211..7531
                     /gene="ytxJ"
                     /locus_tag="POB55_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol system redox-active protein YtxJ"
                     /protein_id="HDI6500390.1"
                     /translation="MAIKLSSIDQFEQVIEENKYVFVLKHSETCPISANAYDQFNKFL
                     YERDMDGYYLIVQQERDLSDYIAKKTNVKHESPQAFYFVNGEMVWNRDHGDINVSSLA
                     QAEE"
     gene            7956..9080
                     /locus_tag="POB55_001424"
     CDS             7956..9080
                     /locus_tag="POB55_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDI6500391.1"
                     /translation="MKVLVAMDEFHGIISSYQANRYVEEAVASQIETADVVQVPLFNG
                     RHELLDSVFLWQSGQKYRIPVHDADMNEVEGVYGQTDTGMTVIEGNLFLKGKKPIVER
                     TSYGLGEMIKHALDNDAKHVVISLGGIDSFDAGAGMLQALGAQFYDDEGRVVDMRQGA
                     GVIKYIRRMDMSNLHPKMETARIQVMSDFSSRLYGKQSEIMQTYDAHQLNHNQAAEID
                     NLIWYFSELFKSELKIAIGPVERGGAGGGIAAVLNGLYQAEILTSHALVDQLTHLENL
                     VEQADLIIFGEGLNENDQLLETTTLRIAELCHKHQKVAIAICATAEKFDLFESQGVTA
                     MFNTFIDMPETYTDFKMGLQIRHYTVQSLKLLKTHFNVEV"
     gene            complement(9262..10488)
                     /gene="pepT"
                     /locus_tag="POB55_001425"
     CDS             complement(9262..10488)
                     /gene="pepT"
                     /locus_tag="POB55_001425"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HDI6500392.1"
                     /translation="MKNQLIDRLTRYTTIDTQSDPKSTTTPSTEKQWDLLHLLEKELQ
                     QLGLPTDLDENGYLFATLESNIDVDVPTVGFLAHVDTSPDFNASNVKPQIIENYDGKP
                     YKLGNTKRVLDPKVFPELNSLVGHTLMVTDGTSLLGADDKAGIVEIMEAICYLQEHPE
                     IKHGTIRIGFTPDEEIGRGPHKFDVDRFNADFAYTMDGSQYGELQYESFNAAEAVITC
                     HGVNVHPGSAKNAMVNAIRLGEQFDSLLPDSEVPERTEGYEGFYHLMNFEGTVEKATL
                     QYIIRDHDKKQFELRKKRILEIRDDINAHFENYPVKVDISDQYFNMAEKILPLPHIID
                     IPKRVFAKLDIPANTEPIRGGTDGSQLSFMGLPTPNIFTGCGNFHGPYEYASIDVMEK
                     AVQVIIGIVEDIAENH"
     gene            complement(10502..10996)
                     /locus_tag="POB55_001426"
     CDS             complement(10502..10996)
                     /locus_tag="POB55_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDI6500393.1"
                     /translation="MLFYLFHFTISFISTVLFSIIFNAPKRLLVACGFVGAIAWTIYQ
                     LTVDLEFGKVGASFLGSLILGLMSHTMSRRYKRPVIIFIVPGIIPLVPGGAAYQATRF
                     LVSNDYTSAVNTFLEVTLISGAIAFGILVSEILYYLYTRIKQLYGKIKGKTYKKSYNM
                     NNRV"
     gene            complement(11014..11775)
                     /locus_tag="POB55_001427"
     CDS             complement(11014..11775)
                     /locus_tag="POB55_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HDI6500394.1"
                     /translation="MPDSITIIDENKVIDVVLIAGRILLESGAETYRVEDTMNRIAHS
                     YGLHNTYSFVSSTAIIFSLNDRTSTRLIRVQERTTDLEKIALTNSLSRKISNKELTID
                     EAKSEFIHLQHASLQYSFLTNFFAAAIACGFFLFMFGGVASDCWIAVIAGGSAFLTFS
                     FVQRYIQIKFFSEFVAAAVVISIAATFTKLGIATNQDIITIASVMPLVPGILITNAIR
                     DLLAGELLAGMSRGVEAALTAFAIGAGVAIVLLII"
     gene            complement(11971..13041)
                     /locus_tag="POB55_001428"
     CDS             complement(11971..13041)
                     /locus_tag="POB55_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDI6500395.1"
                     /translation="MFEAFIYNISVIVAGIYLFHRLQYSENKRMVFSKAYVTVLMTIV
                     SLLLSVYPIPYREDYLIHLTFVPLLFLGRFTNMVYTLSATVIVAIVEIVVFNNSIMYG
                     VTLIVIAAVTSAIGPFLKQNDVLSLLILNVVTIIILFGVALVSPIYTLSEVIILIPIS
                     LIITLASAITFVDIWHFFSLVNRYENEDKYDYLTGLGNVKEFDRHLNEISRKAEKEHQ
                     SIALLLIDIDGFKDVNDTYSHKSGDAVLKQMSQLLKNYVPNQFKIFRNGGEEFSVVIH
                     NYSLDQSVKLAENIRSGVEKSSFHLPNKEVIKLSVSIGVGYLTDDDPKSQRKVFKDAD
                     DMVHVAKNQGRNKVMFNPIINL"
BASE COUNT         4604 a         1886 c         2278 g         4391 t
ORIGIN      
        1 tttaaaggga gaaaaatttg taatgaagta tttattaaag ggaaatattt tgcttctatt
       61 actaatattg ttgacaatta tttcgttgtt cataggtgtg agtgaactat caattaaaga
      121 tttactacat ttaactgagt cacagcggaa tattttattc tcaagccgaa taccaaggac
      181 gatgagtatt ttaattgctg gaagttcgtt ggctttagca ggcttgataa tgcaacaaat
      241 gatgcaaaat aagtttgtta gtccgactac agctggaacg atggaatggg ctaaactagg
      301 tattttaatt gctttattgt tctttccaac cggtcatatt ttattaaaac tagtatttgc
      361 tgttatttgc agtatttgcg gtacgttttt atttgttaaa atcattgatt ttataaaagt
      421 gaaagatgtc atttttgtac cgcttctagg aattatgatg ggtgggattg ttgcaagttt
      481 cacaaccttc atctcattgc gcacgaatgc tgttcaaagc attggtaact ggcttaacgg
      541 gaactttgcc attatcacaa gtggacgcta tgaaatttta tatttaagta ttcctctttt
      601 agcattgaca tatctttttg ctaatcattt cacgattgta ggaatgggta aagactttac
      661 taataattta ggtttgagtt acgaaaaatt aattaacatc gcattgttta ttactgcaac
      721 tattacagca ttggtagtgg tgactgttgg aacattaccg ttcttaggac tagtaatacc
      781 aaatattatt tcaatttatc gaggtgatca tttgaaaaat gctatccctc atacgatgat
      841 gttaggtgcc atctttgtat tattttctga tatagttggc agaattgttg tttatccata
      901 tgaaataaat attggtttaa caataggtgt atttggaaca atcattttcc ttatcttgct
      961 tatgaaaggt aggaaaaatt atgcgcaaca ataataaaaa aataatgctt ttaattgcag
     1021 taacgttatt aattagtatg ctgtacttat ttgtaggtat tgattttgaa atatttgaat
     1081 atcaattttc aagtcgttta agaaagttca tattaattat tttagtaggt gctgccattg
     1141 caacttcagt ggtgattttt caagcgatta caaataaccg tctattgaca ccatcaataa
     1201 tggggttaga tgcagtttat ttatttatca aagtattgcc agtcttttta tttggaattc
     1261 aatcggtatg ggttactaat gtatatttga actttatatt aacacttata acgatggtgt
     1321 tattcgcact aatcctattc caaggtatct ttaaaattgg acatttttca atttatttta
     1381 tcttacttat tggtgtcctt ttaggaacat tttttagaag cataacaggt tttattcaac
     1441 tgattatgga tcctgagtca tttttagcaa tacaaagtag tatgtttgct aattttaatg
     1501 cttctaattc gaatttagtt attttctcag cagtgctatt agtaatctta ttagtcatta
     1561 caattttact attgccttat ttagatgtat tgcttttagg tcgtgctgaa gcaattaatc
     1621 ttgggatatc gtatgaaaaa ttaacgcgaa ttctacttgt aatagtctca gttttagttt
     1681 ctgtgtcaac tgcattagta ggaccaatta catttttagg tttattaact gtaaatctag
     1741 cgcatgaact aatgaagacg tatgaacata agtatatttt aattgcgaca atttgcttga
     1801 gttggattag tttatttagt gcgcaatggg tagttgaaaa tgtgtttgaa gctacgacag
     1861 aaatgagtat acttattgat ttgattggtg gaagttattt catttatcta ttagttagaa
     1921 ggagaaatgc gcaatgattc aagttgaaaa tttaactaaa actataaata atcaaatgat
     1981 attggaagat attagcatag atatcgaaaa aggtaaattg acttctttaa ttggacctaa
     2041 tggtgcgggt aagagtactt tactttcagc gatatgtagg ttaattcgtt ttgataacgg
     2101 tgaagtgaaa atagatggac agctcatgtc tgattataaa aataatgact tgtcgaaaaa
     2161 aatatctata ttaaaacaaa caaaccatac tgaaatgaat attacggtag agcagttggt
     2221 aaactttgga cgattccctt attctaaagg tcgtttgacg aaagaggatc atgatattgt
     2281 caatgatgcg ctagatttgt tgcaactaca agatatcaga aatcgtaata ttaagtcatt
     2341 atctggtgga caacgtcagc gtgcatacat tgcaatgaca atagcacaag atactgaata
     2401 tattttgcta gatgaaccat taaataattt agatatgaag catgctgttc aaattatgca
     2461 aacgttaaaa atgttagcgc ataaaatgaa taaagcgatt gtcattgtgt tacatgatat
     2521 taactttgcg tcctgttatt cagatcagat tgtagcattg aaaaacggac aactagttaa
     2581 gtcagatttg aaagataatg tcattcaaag tagtgtttta agtgatttat atgacatgaa
     2641 tattcaaatt gaacatataa gaaatcaaag gatttgttta tattttaagg attgataatt
     2701 tggagacact ttaaaggggt gatgcgccaa ttaaagaagg gttaaacgta aagcatttat
     2761 ttatatttca catcaagcac acagattaag ccaaaagagg agaatattat attatgaaga
     2821 aaacagtctt atatttagta ttagcagtaa tgtttttatt agcggcatgc ggtaacaatt
     2881 ctgataaaga acaatcaaaa tcagaaacta aaggttctaa agatacagta aaaattgaaa
     2941 ataactataa aatgcgtggc gagaaaaaag atggtagtga cgctaaaaaa gttaaagaaa
     3001 ctgttgaagt accaaaaaat cctaaaaatg cagttgtgtt agactatggc gcattagatg
     3061 taatgaaaga aatgggctta tcagataaag taaaagcatt acctaaaggg gaaggcggta
     3121 agtcattacc gaatttctta gaatcattta aagatgataa atatacaaac gttggtaatt
     3181 taaaagaagt gaattttgat aaaattgctg cgacgaaacc cgaagtaatc tttatctctg
     3241 gacgtacagc taatcaaaag aatttagatg aattcaaaaa agctgcacct aaagcgaaaa
     3301 ttgtttatgt tggtgcagat gaaaagaact taattggttc aatgaaacaa aacactgaaa
     3361 atatcggtaa aatttacgat aaagaagata aagctaaaga attaaataaa gatttagata
     3421 acaaaattgc ttcaatgaaa gataaaacga aaaacttcaa taaaactgtt atgtatttac
     3481 tagttaacga aggtgaatta tcaacatttg gacctaaagg tcgttttggt ggattagttt
     3541 acgatacatt aggattcaat gcagttgata aaaaagtaag taatagcaat catggacaaa
     3601 atgtttctaa cgaatatgtt aataaagaaa atccagatgt tattttagcg atggatagag
     3661 gtcaagcgat aagtggtaaa tcaactgcga aacaagcatt aaataatcct gtattaaaaa
     3721 atgttaaagc aattaaagaa gacaaagtat ataatttaga tcctaaatta tggtactttg
     3781 cagctggatc aactacaact acaattaaac aaattgagga acttgataaa gttgtaaaat
     3841 aattttaaaa gaggggaaca atggttaaag gtcttaatca ttgctcccct cttttcttta
     3901 aaaaaggaaa tctgggacgt caatcaatgt cctagactct aaaatgttct gttgtcagtc
     3961 gttggttgaa tgaacatgta cttgtaacaa gttcatttca ataccagtgg gctccaaaca
     4021 tagagaaatt tgattttcaa tttctactga caatgcaagt tggcggggcc caaacataga
     4081 gaatttcaaa aaggaattct acagaagtgg tgctttatca tgtctgaccc actccctata
     4141 atgttttgac tatgttgttt aaatttcaaa ataaatatga tagtgatatt tacagcgatt
     4201 gttaaaccga gattggcaat ttggacaacg ctctaccatc atatattcat tgattgttaa
     4261 ttcgtgtttg catacaccgc ataagattgc tttttcgtta aatgaaggct cagaccaacg
     4321 cttaatggcg tgcttttcaa actcattatg gcacttatag catggatagt atttattaca
     4381 acatttaaat ttaatagcaa taatatcttc ttcggtaaaa taatggcgac agcgtgtttc
     4441 agtatcgatt aatgaaccat aaactttagg catagacaaa gctccttaac ttacgattcc
     4501 tttggatgtt caccaataat gcgaacttca cgatttaatt caatgccaaa tttttctttg
     4561 acggtctttt gtacataatg aataaggttt tcataatctg tagcagttcc attgtctaca
     4621 tttaccataa aaccagcgtg tttagttgaa acttcaacgc cgccaatacg gtgaccttgc
     4681 aaattagaat cttgtatcaa tttacctgca aaatgaccag gcggtctttg gaatacacta
     4741 ccacatgaag gatactctaa aggttgttta gattctctac gttctgttaa atcatccatt
     4801 ttagcttgta tttcagtcgt tttaccagga gctaaagtaa atgcagcttc taatacaact
     4861 aagtgttctt tttgaataat gctattacga taatctaact ctaattcttt tgttgtaagt
     4921 ttaattaacg agccttgttc gtttacgcaa agcgcatagt ctatacaatc tttaacttcg
     4981 ccaccataag cgccagcatt catatacact gcaccaccga ttgaacctgg aataccacat
     5041 gcaaattcaa ggccagtaag tgcgtaatca cgagcaacac gtgagacatc aataattgca
     5101 gcgccgctac cggctattat cgcatcatca gatacttcga tatgatctag tgataataaa
     5161 ctaattacaa tgccacgaat accaccttca cggataataa tatttgagcc atttcctaaa
     5221 tatgtaacag gaatctcatt ttgataggca tatttaacaa ctgcttgtac ttcttcattt
     5281 ttagtagggg taatataaaa gtcggcatta ccacctgttt tagtataagt gtatcgtttt
     5341 aaaggttcat caactttaat tttttcattt gggataagtt gttgtaaagc ttgatagatg
     5401 tctttattta tcacttctca gtacatcctt tctcatgtct ttaatatcat atagtattat
     5461 accaatttta aaattcattt gcgaaaattg aaaagaaagt attagaatta gtataattat
     5521 aaaatacgga attattgtcg ttataagtat tttttacata gtttttcaaa gtattgttgc
     5581 ttttgcatct catattgtct aattgttaag ctatgttgca atatttggtg cttttttgta
     5641 tggaattgca aagcaatatc atcattagtt gataagaggt aatcaagtgc aagataagat
     5701 tcaaatgttt gggtattcat ttgaatgata tgtagacgca cctgttgttt tagttcatga
     5761 aaattgttaa acttcgccat cataactttc ttattatatt tatgatgcaa gcgataaaac
     5821 cctacataat ttaagcgttt ttcatctaag gatgtaatat catgcaaatt ttctacacct
     5881 actaaaatat ctaaaattgg ctctgttgaa tatttaaaat gatgcgtacc gccaatatgt
     5941 tttgtatatt ttactgggct gtctaagagg ttgaataata atgattcaat ttcagtgtat
     6001 tgtgattgaa aacaattagt taaatcacta ttaatgaatg gttgaacatt tgaatacatg
     6061 ataaactcct ttgatattga aatttaattt aatcacgata aagtctggaa tactataaca
     6121 taattcattt tcataataaa catgtttttg tataatgaat ctgttaagga gtgcaatcat
     6181 gaaaaaaatt gttattatcg ctgttttagc gattttattt gtagtaataa gtgcttgtgg
     6241 taataaagaa aaagaggcac aacatcaatt tactaagcaa tttaaagatg ttgagcaaaa
     6301 acaaaaagaa ttacaacatg tcatggataa tatacatttg aaagaaattg atcatctaag
     6361 taaaactgat acaactgata aaaatagtaa agaatttaag gcactacaag aagatgttaa
     6421 aaaccatctc atacctaaat ttgaagcata ttataagtca gcaaaaaatt tgcctgatga
     6481 tacaatgaaa gttaagaaat taaaaaaaga atatatgacg cttgcaaatg agaagaagga
     6541 tgcgatatat caattaaaaa aattcatagg tttatgtaat caatctatca agtataacga
     6601 agacatttta gattatacaa aacaatttga aaaaaataga tacaaagttg aatcagaaat
     6661 taaattagct gataataaaa gtgaagcaac taatcttacg acaaaattag aacataataa
     6721 taaagcgtta agagatactg cgaagaagaa cctagatgat agtaaagaaa atgaagtaaa
     6781 aggcgcgatt aaaaatcaca ttatgccaat gattgaaaag caaattaccg atattaacca
     6841 aactaatatt agtgataagc atgttaataa tgcaaggaaa aacgcaatag aaatgtatta
     6901 cagtctgcag aactattata atacacgtat tgaaacaata aaggttagtg agaagttatc
     6961 aaaagtcgat gtagataagt tgccgaaaaa gggtatagat ataactcacg gcgataaagc
     7021 ctttgaaaaa aagcttgaaa aattagaaga aaaataacta taatcatttt tcaaagttaa
     7081 aaattttgaa tttatggtta acatgtcaac ttactatgtg tataatggta aacattgata
     7141 ttaactatat gtataaaaat gtcacgcaga tgctatttaa atgtgataaa tatttttaga
     7201 ggtgaataga gtggctataa agctaagttc aattgaccaa tttgaacagg ttattgagga
     7261 aaataaatat gtttttgtat taaaacatag tgaaacttgt ccaatatcgg caaatgcgta
     7321 cgatcaattt aataaatttt tatatgaacg cgatatggac ggttattatt tgattgtcca
     7381 acaagaacgc gatttgtcag attatattgc taaaaaaacg aacgttaaac atgaatcacc
     7441 tcaagcattt tattttgtaa atggtgaaat ggtttggaat cgagaccacg gtgatatcaa
     7501 tgtgtcgtca ttagcacaag cagaagaata atgaaactat agggttggaa cattttgcct
     7561 tacactacta gacgtgaata gcacaactta aattcgtgtg aatcagagta gtttggctat
     7621 aatgatgttc tgacctttta ttttatgtca cctttagaag cagttaagtt agtacttttt
     7681 tacaaacata tgtataatgt attcgagtat ttttattgaa aatattttgg aaaacgacga
     7741 atccaataag aaaatttaaa catgatttgt aagttagttt aataggaaat atatgctaaa
     7801 ccaaaagaag catattgtta tttactggaa taattaataa tcatgtcatg ttaaatgttc
     7861 gcatataatc acgagataaa atctaaaatt taagattaat cttttatgaa taaaaaacgt
     7921 atcacaacaa ataataaagt aaggtggtca aggttatgaa agtattagta gccatggatg
     7981 agtttcatgg aattatttcg agttatcaag ctaatagata tgttgaagag gcagttgcaa
     8041 gccaaattga aactgcagat gtagttcaag taccattgtt taatggaaga catgaattat
     8101 tagattctgt atttttatgg caatctgggc aaaagtatcg tataccagta catgatgcag
     8161 atatgaatga agttgaaggt gtttacggac aaactgatac agggatgacc gttatcgagg
     8221 ggaatttatt tttaaaaggt aaaaaaccaa ttgttgaacg aacaagttat ggtttaggag
     8281 aaatgattaa acatgcatta gataacgacg caaaacatgt tgtaatttca ctaggtggga
     8341 ttgatagttt tgatgctggt gcaggtatgt tacaagcatt aggtgctcaa ttctatgatg
     8401 acgaagggcg tgtcgtagat atgagacaag gtgctggtgt aattaaatat attcgtcgta
     8461 tggatatgtc gaacttacac cctaaaatgg aaacagcaag aattcaagta atgtcggatt
     8521 tttcaagtcg attatatggt aagcaaagtg aaatcatgca aacttatgat gcgcatcagt
     8581 tgaatcataa tcaagcagca gaaatcgata atttaatttg gtattttagt gagttattta
     8641 aaagtgaatt gaaaattgca attggtccag ttgaacgtgg tggtgctggt ggtggaattg
     8701 cagcagtctt gaatggactg tatcaagctg aaatattaac cagtcatgca ttagtagacc
     8761 aactaacaca tttagaaaat ttagttgaac aagcggattt aattattttt ggagaagggt
     8821 taaatgaaaa tgatcagttg ctagaaacga caacattgcg tattgcagaa ctttgtcata
     8881 aacatcaaaa ggttgccatt gcaatttgtg caactgctga aaagtttgat ttatttgaat
     8941 cacaaggggt tacagcaatg tttaatacat ttatcgatat gccagaaact tatactgact
     9001 ttaaaatggg gttacaaatt aggcattata cggttcagtc tttaaaactg ttgaaaacac
     9061 attttaatgt tgaggtttag taaagaagga ctaaattggt gatgctgtca tgttggttaa
     9121 taacatttat gatggttagc aaaacgaatt agaagatcga aagtatacgt aaaaaatatg
     9181 aaaaatcacg ctatcattgc actgaatgtt agcgtgattt ttttattaat taagcctgag
     9241 ttggtatata atcgttggtt tttagtgatt ttcagcgata tcttctacaa ttccaatgat
     9301 tacttgtact gctttttcca taacatcaat ggatgcatat tcatatgggc cgtggaagtt
     9361 accgcaacct gtaaagatgt ttggagttgg taaccccata aatgacaatt gtgaaccatc
     9421 tgtaccaccg cgaataggtt cagtgtttgc tggaatatct aatttggcaa agacacgttt
     9481 aggtatatca ataatatgag gcaatggtaa tattttttct gccatattga aatattgatc
     9541 cgatatatca actttaactg gataattttc aaaatgggca ttgatatcgt cacgtatttc
     9601 taaaatacgt ttcttacgca attcgaattg ttttttatca tgatcacgaa taatgtattg
     9661 caaagttgct ttttcaacag ttccttcaaa gttcattaag tgataaaagc cttcgtatcc
     9721 ttctgttcgc tccggaactt cactatcagg tagcaaacta tcgaattgtt cacctaaacg
     9781 tattgcgttt accattgcat ttttagctga accaggatga acatttacac cgtggcatgt
     9841 aataaccgct tcagcagcgt taaagctttc atattgtaat tctccatatt gactaccatc
     9901 catagtataa gcaaaatcag cattgaagcg gtcaacatca aatttatgtg gaccacgacc
     9961 gatttcttcg tctggtgtaa atccaatgcg aatggtacca tgtttaattt ctggatgttc
    10021 ttgtaaataa caaatagctt ccataatttc cacaataccc gctttatcgt ctgcacctag
    10081 taacgatgta ccatcagtta ccattaatgt atgaccaact aaactgttaa gttctggaaa
    10141 tactttagga tctaagacac gtttagtatt gcctagtttg tatggcttac catcatagtt
    10201 ttcaataatt tgcggtttaa catttgaagc attgaaatca ggtgatgtat caacatgcgc
    10261 caaaaatcca actgttggga catcgacatc gatgttactt tctaatgtag caaataagta
    10321 gccattttca tctaaatcag ttggcaatcc taattgttgt aattcttttt ctaataaatg
    10381 taacaaatcc cattgctttt cagttgaagg tgttgttgta gattttggat cagattgcgt
    10441 atcaattgtc gtatatcttg ttaatctatc tatcaattgg ttcttcatta tattcgaccc
    10501 cttaaactct attattcatg ttgtaagatt ttttatatgt cttacctttg attttaccat
    10561 acagttgttt gatacgtgtg tataggtaat atagaatttc agaaactaat ataccgaaag
    10621 caatcgcacc tgaaatcagt gtaacttcta aaaatgtatt tacagcactt gtataatcat
    10681 ttgatactaa aaaacgagtc gcttgataag ctgcaccacc aggtactaat ggtataatgc
    10741 ctggcactat gaatataatt accggtcgtt tatatctgcg gctcatagta tgactcatta
    10801 agcctaaaat taagcttccc aaaaatgaag cgccaacttt tccaaactct aaatctaccg
    10861 ttaattggta aatcgtccat gcaatggcac ccacaaatcc acatgctact aagaggcgtt
    10921 tgggtgcatt gaaaatgata gagaaaagta ctgttgatat aaagctgatt gtaaaatgaa
    10981 ataaataaaa tagcatgctt taacagtcct tccttaaatg attaataaaa cgattgcgac
    11041 accagcaccg attgcgaatg ctgttaatgc agcttcaaca ccacgagaca tacctgcaag
    11101 taattcaccc gctaataaat ctcgaatggc attggtaatt aatataccag ggacaagtgg
    11161 catgacactg gctatagtaa tgatatcttg attggttgca atgcctaatt tagtaaatgt
    11221 ggctgcaatg gatatgacca cagcggctgc aacaaactct gagaaaaatt taatttgtat
    11281 atagcgttgc acaaagctga atgttaaaaa tgcggatccg ccagcaatga ctgcaatcca
    11341 acaatctgat gcgacaccac caaacataaa taggaagaag ccacatgcaa tggcagctgc
    11401 aaagaaattc gttaaaaaag aatattgtaa tgatgcatgc tgtaaatgaa taaattcaga
    11461 tttagcttca tcaattgtga gttctttatt tgatatttta cgtgaaagac tattcgttaa
    11521 agcgattttc tctaaatctg ttgtacgctc ttgtacacga attaatcttg tacttgttcg
    11581 atcgtttaat gaaaaaataa ttgcagttga actgacaaaa ctatatgtat tatgaagacc
    11641 ataactatgt gcgatacggt tcattgtatc ttcaactcga tatgtttcag cacctgattc
    11701 aagtaaaatt ctacctgcaa ttaatacaac atcaatcact ttgttttcat ctataattgt
    11761 gattgaatct ggcatatcaa ttcacctcca atgatatgtg ttatttattt gaacaattga
    11821 agtttacaac ttgttgttgc aactttcaat agtgagactt tgtgttagta tgatgaactt
    11881 gtatggttca aatttaaata agaaaaactg ttaatctttg ctattatact atgatttaat
    11941 aatagcaaag gattaacagt tttgtcgttg ttataaattg ataatagggt taaacattac
    12001 tttgtttcgc ccttgatttt tggctacatg caccatatcg tctgcatctt taaacacttt
    12061 acgctgtgat tttggatcgt catctgttaa atatccaaca ccgatagaca ctgacaattt
    12121 aataacttct ttgtttggta aatggaatga tgatttttca acacccgaac gaatattttc
    12181 agccaattta acactttgat caagtgaata attgtgaatg acaactgaga actcttcgcc
    12241 accatttcta aaaattttaa attgattcgg cacatagttt ttaagtaatt gagacatttg
    12301 ttttaataca gcatcacctg atttgtgtga gtaggtatca ttgacatctt taaatccatc
    12361 gatatcgatt aataataaag cgatactttg atgttctttt tcagcttttc gtgaaatttc
    12421 atttaaatgt ctatcaaatt cttttacatt acctaagcct gttaagtaat catatttatc
    12481 ttcgttttca taacgattta cgagtgagaa gaaatgccaa atatcgacaa atgttatcgc
    12541 tgaagctaaa gtgataatta atgaaattgg tattaaaatg ataacttccg atagtgtgta
    12601 aataggactc actaacgcga caccaaataa aatgattatt gtaacaacat taagtattaa
    12661 taatgatagc acatcatttt gttttaaaaa tggtccaata gcacttgtta ctgcagcaat
    12721 aacaatcaac gtaacaccgt acataatcga gttgttaaat actacaattt caacaattgc
    12781 tacaattact gtggcagata atgtatagac catatttgta aatctaccta aaaacaataa
    12841 aggaacgaat gttaagtgaa ttaaataatc ttcacgataa gggatagggt agacagataa
    12901 taataatgat acgattgtca ttaaaacagt gacataagcc ttagaaaaaa ccatacgttt
    12961 gttttctgaa tactgtaagc gatggaataa atagattcca gcgactataa cagatatatt
    13021 gtatataaat gcttcgaaca tgtctgaatc gactccttta attgaccact agctattgta
    13081 agtgaaaact tacaatttgt cattagttta catataaaat taatgtatga tatagacttt
    13141 gatgttaaaa tgttgcctt
//
LOCUS       DAMZKC010000068        13035 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.068, whole genome shotgun sequence.
ACCESSION   DAMZKC010000068 DAMZKC010000000
VERSION     DAMZKC010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13035
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.068"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(75..259)
                     /locus_tag="POB55_001429"
                     /pseudo
     CDS             complement(75..259)
                     /locus_tag="POB55_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500888.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(348..1412)
                     /locus_tag="POB55_001430"
     CDS             complement(348..1412)
                     /locus_tag="POB55_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HDI6500396.1"
                     /translation="MLTVDQVKELVGEIKDPIIDVPLKETEGIVEVSIKEEKEHVSVK
                     LAMAQLGGAPQLDLQMAVVNALKENGAKTVGIRFETLPEEKVNQFKPKEENKPKTIEG
                     LLSQNNPVEFIAIASGKGGVGKSTVAVNLAVALAREGKKVGLVDADIYGFSVPDMMGI
                     DEKPGIKGKEVIPVERHGVKVISMAFFVEENAPVIWRGPMLGKMLTNFFTEVKWGDIE
                     YLILDLPPGTGDVALDVHTMLPSSKEIIVTTPHPTAAFVAARAGAMAKHTDHSILGVI
                     ENMSYFESKETGNKEYVFGKGGGTKLADELNTQLLGELPLEQPSWNPKDFAPSIYQSD
                     DRLGKIYSSIAQKVIAATNK"
     gene            complement(1560..3002)
                     /gene="lmrS"
                     /locus_tag="POB55_001431"
     CDS             complement(1560..3002)
                     /gene="lmrS"
                     /locus_tag="POB55_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter LmrS"
                     /protein_id="HDI6500397.1"
                     /translation="MAKVELTTRRRNFIVAVMLISAFVAILNQTLLNTALPSIMRELN
                     INESTSQWLVTGFMLVNGVMIPLTAYLMDRIKTRPLYLAAMGTFLLGSIVAALAPNFG
                     VLMLARVIQAMGAGVLMPLMQFTLFTLFSKEHRGFAMGLAGLVIQFAPAIGPTVTGLI
                     IDQASWRVPFIIIVGIALVAFVFGLVSISSYNEVKYTKLDKRSVMYSTIGFGLMLYAF
                     SSAGDLGFTSPIVIGALIISMVIIYLFIRRQFNITNALLNLRVFKNRTFALCTISSMI
                     IMMSMVGPALLIPLYVQNSLSLSALLSGLVIMPGAIINGIMSVFTGKFYDKYGPRPLI
                     YTGFTILTITTIMLCFLHTDTSYTYLIVVYAIRMFSVSLLMMPINTTGINSLRNEEIS
                     HGTAIMNFGRVMAGSLGTALMVTLMSFGAKIFLSTSPSHLTATEIKQQSIAIGVDISF
                     AFVAVLVMAAYVIALFIREPKEIESNRRKF"
     gene            complement(3325..3798)
                     /gene="sepA"
                     /locus_tag="POB55_001432"
     CDS             complement(3325..3798)
                     /gene="sepA"
                     /locus_tag="POB55_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter SepA"
                     /protein_id="HDI6500398.1"
                     /translation="MIVNYLKHKFYNLLTTMVVLFIFVLSGAIFLTFLGFGLYGLSRI
                     LIYFRLGDFTYNRSMYDNLLYYGSYIIFGYFIIFAVEHLMDYFRKMLPENAYFRGATF
                     HLISYTVATTLFYFIIHLNYVYINIDFWVIMVIIGFLYVCKLQFYPESKNLNNRK"
     gene            complement(3897..5240)
                     /locus_tag="POB55_001433"
     CDS             complement(3897..5240)
                     /locus_tag="POB55_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6500399.1"
                     /translation="MRLKSIITVIALILIMFMSAIESSIISLALPTIKQDLNAGNLIS
                     LIFTAYFIALVIANPIVGELLSRFKIIYVAIAGLLLFSIGSFMCGLSTNFTMLIISRV
                     IQGFGSGVLMSLSQIVPKLAFEIPLRYKIMGIVGSVWGISSIIGPLLGGGILEFATWH
                     WLFYINIPIAIIAIILVIWTFHFPEEETVAKSKFDTKGLTLFYVFIGLIMFALLNQQL
                     LLLNFLSFILAIVVAMCLFKVEKHVSSPFLPVVEFNRSITLVFITDLLTAICLMGFNL
                     YIPVYLQEQLGLSPLQSGLVIFPLSLAWITLNFNLHRIEAKLSRKVIYLLSFTLLLVS
                     SIIISFGIKLPVLIAFVLILAGLSFGYIYTKDSVIVQEETSPLQMKKMMSFYGLTKNL
                     GASIGSTIMGYLYAIQSGIFGPNLHNVLSAVAVISIGLIVLWVVFFKEQSSQSKE"
     gene            complement(5417..6100)
                     /locus_tag="POB55_001434"
     CDS             complement(5417..6100)
                     /locus_tag="POB55_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HDI6500400.1"
                     /translation="MSKSTKKSNDTIVETFKDIIPLSFGEEIGNAASHGVAALLTLLV
                     LPYAAVHSYIAHGTLASVSMSIYVISIFMMFISSTIYHSMQNETPHKYILRIIDHSMI
                     YVAISGTYTPILLTVVGGWIGWTVFILLWGTTLWGILYKSIAVKVNQKLSLIVYLVMG
                     WVGIILLPIIFIRTSWIFILFIFLGGVSYTIGAWFYAQKNRPYFHMIWHIFIVIASLL
                     HLIAILYFM"
     gene            complement(6119..7306)
                     /locus_tag="POB55_001435"
     CDS             complement(6119..7306)
                     /locus_tag="POB55_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDPGP type 1 family protein"
                     /protein_id="HDI6500401.1"
                     /translation="MLDKNQLAKYKQDHLCEYEKIMSNNEKEALEEKVASLDLDFIAK
                     LYNDLYINKKTIDDVSAVSEVKYDIKSQMSDDEIKRLEEQGLQAIKEGQFAVLLMAGG
                     QGTRLGYKGPKGSFEIEGVSLFELQANQLKTLNHQSGHTIQWYIMTSDINHEETLAYF
                     EAHSYFGYDQEAIHFFKQDNIVALSEEGKLILNQQGRIMETPNGNGGVFKSLDKAGYL
                     EEMSNNGVKYIFLNNIDNVLVKVLDPLFAGFTVEHDYDITSKTIQPKPGESVGRLVNV
                     DCKDTVLEYSELDPEVANQFNNANIGIHAFKLGFILNAVNRELPYHLAIKNLKQLDEN
                     FGVIEQPTLKFELFYFDIFTYGTSFVTLQVPREEEFSPLKNKEGKDSVATATEDLRRM
                     GLI"
     gene            complement(7350..7865)
                     /locus_tag="POB55_001436"
     CDS             complement(7350..7865)
                     /locus_tag="POB55_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDI6500402.1"
                     /translation="MTGKTHASCGMLVGALTTQYFQTDIFSSVTVIILATLASLLPDI
                     CHTQSKIGRRFKVISFFVRLIFGHRTFTHSILFIAIIAFLLQIIQTPNYYMAAIIIGL
                     VSHVILDMITPRGVKLFYPLPFNVKTPIQFKTGGLVDLSLATALMVGTVYVLFQPFIN
                     DIISNWNTKFL"
     gene            8042..8302
                     /locus_tag="POB55_001437"
     CDS             8042..8302
                     /locus_tag="POB55_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500403.1"
                     /translation="MAMTVKKDNNEVRIQWRVADIKIPTSEIKNITQDQDIHAVPKLD
                     SKDVSRIGSTFGKTNRVIIDTEDHEYIIYTQNDQKVYNELTK"
     gene            complement(8434..9804)
                     /locus_tag="POB55_001438"
     CDS             complement(8434..9804)
                     /locus_tag="POB55_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001670170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HDI6500404.1"
                     /translation="MDSFSRKEIVIGRLKFITMSLIGILLFLVPIPVEQDGQKQTTLP
                     VAFLAGVLKDVLGGVMPFLIVTIITLSGIITLICSTILKDKLKPDGLMNNAFNVRIGW
                     LILRILAVVFAWMTFLRIGSKVIYSDETGGLLFSSLLPTLVAVFLFAALFLPLLMEYG
                     LLEMLGPIFRPVMRPLFTLPGRSTVDNLASFIGDGTVGVLITSRQYGEGYYSRREATV
                     ISTTFSVVSITFAIVVAETVHMQNQFFAFYLSVIVSCLVAAVIMPRIWPLNKIPDEYA
                     KEVPESARTEALPEGKTALRHGFDTATEVGIKAPGVIDFFKSGLKTVIDMWFVILPVV
                     MSIGTIATIIANYTPFFVILGKPFVPFLELMQIPEAAQASQTIIIGFADMFLPSILIE
                     GVQNDITRFVIGALSISQLIYLSEVGGVILGSKIPVSIGKLFMIFLIRTIITLPIISL
                     MAHLLL"
     gene            complement(10040..11008)
                     /locus_tag="POB55_001439"
     CDS             complement(10040..11008)
                     /locus_tag="POB55_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDI6500405.1"
                     /translation="MMTTKIIRRYSLITILLIVSIFISLCVGSVMIHPIDAIKGIFTQ
                     DDFILNEYRIPRTLLGIIIGSSLAISGAIIQAVIRNPLASPDVIGISKGASLAAVIII
                     MTFPTAPLFVLPIGSFLGAFAVSLFLSFLISKYDVKGSKLALIGLAIGAICTAIVQFL
                     LIRNPLEANKALVWLTGSLYGHDMSNIYTILPWFLITIPIIILLSYQLDVLNLGDAVA
                     TALGLKVKTIKMLLLILAVILAGSAISVVGGISFLGLIAPHIARQLIGNKTLHVIIMS
                     GLIGAILLTFGDGLARGIHPPLDIPVGVIIAIIGAPYFLILLRRMK"
     gene            complement(11005..12036)
                     /locus_tag="POB55_001440"
     CDS             complement(11005..12036)
                     /locus_tag="POB55_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDI6500406.1"
                     /translation="MAIKEISSQSAIDHKRKRRTTLTYIVSLCFLFICIYLNMAIGSS
                     KINFSDIVHYVTGHTDTKATFLLHNVRMPRMIAGLFIGGALAVSGLLMQAMTRNPLAS
                     PKIFGVSSGASFFIVFVTIIIPSLEYYALYLGVIGAFIGGLTVYTLSGATKGMTPIKL
                     ALAGMAIHLFFSSMTEGIIILNENSNEQVMFWLVGSLSSMKWDEILTILPWLIGALIV
                     TIFIGRQLTIMELGDDIAKGLGQNINKVRIIIGLLVIILTGMSVSVAGPIGFVGLIVP
                     HIVKRYVSKNYLVMIPLTFIIGADLLLLSDVLSRLITYPYESPVGIVTSFVGALYFLF
                     ITIKGVKRI"
     gene            complement(12048..13031)
                     /locus_tag="POB55_001441"
     CDS             complement(12048..13031)
                     /locus_tag="POB55_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDI6500407.1"
                     /translation="MRGLKTFSILGLIVALLLVAACGNTDNSSKKESSTKDTISVKDE
                     NGTVKVPKDAKRIVVLEYSFADALAALDVNPVGIADDGKKKRIIKPVREKIGDYTSVG
                     TRKQPNLEEISKLKPDLIIADSSRHKGINKELNKIAPTLSLKSFDGDYKQNINSFKTI
                     AKALNKEKEGEKRLAEHDKLINKYKDEIKFDRNQKVLPAVVAKAGLLAHPNYSYVGQF
                     LNELGFKNALSDDVTKGLSKYLKGPYLQLDTEHLADLNPERMIIMTDHAKKDSAEFKK
                     LQEDATWKKLNAVKNNRVDIVDRDVWARSRGLISSEEMAKELVELSKKEQK"
BASE COUNT         4592 a         2440 c         1738 g         4265 t
ORIGIN      
        1 atgttcaatc tcttttattc tacttcattt atttcttgaa gtcaataact ttttgaacga
       61 ttactttatt tattctatat tgtttttata gttatttcaa tggtaagttt tacacttttg
      121 aaattcttct ttaaaaacaa ctgcgtcgtt tttgacgact ttatcatatt atcaactttg
      181 gaatttaaag tcaataactt ttttaaaact ttttgtgttc acaacccgct tctttttcaa
      241 cgcgtttatt gcttaacaca agaacttatt ttaccagcat tccaaaacaa atcaacataa
      301 aaacgtacaa aataaaagta attttgtacg tttagcatat attataccta tttatttgta
      361 gcagctataa ctttttgtgc aatcgagcta taaattttac ctagacgatc atctgattga
      421 tatattgacg gtgcaaaatc ttttggattc caagatggtt gctctaaagg taattcccca
      481 agtaattgag tattaagttc atcagctaac ttagtaccgc cacctttgcc aaagacatat
      541 tctttattac ccgtctcttt actttcaaaa taactcatgt tttcaattac gccaagaata
      601 gaatgatccg tatgttttgc catcgcacct gcgcgagctg caacaaatgc tgctgtagga
      661 tgaggtgtcg ttacaataat ttccttactt gaaggtaaca tcgtatgaac atctaaagct
      721 acatctcctg ttccaggtgg aagatcgagt attaaatatt caatgtctcc ccatttaact
      781 tctgtaaaga aattcgtcaa cattttacct aacattggcc ctctccatat aactggcgca
      841 ttttcttcca caaaaaaggc cattgatata actttaacgc catgacgttc aactggaatt
      901 acttccttcc ctttaattcc aggcttttca tcaataccca tcatatctgg tacactaaat
      961 ccatatatat cggcatctac taatccgact tttttccctt cacgagctaa ggcaacggct
     1021 aaatttactg caacagtaga tttaccgaca ccacctttac cggaggctat tgcaataaat
     1081 tcgactggat tattttgaga taataggcct tctatcgttt taggtttatt ttcttctttt
     1141 ggtttaaatt gatttacttt ttcttccggc aatgtttcaa atcgtatacc gaccgttttc
     1201 gcaccgtttt cttttaatgc attaacaaca gccatctgta aatctaattg cggtgcacca
     1261 cctaattgtg ccattgctaa tttaacactc acatgttctt tttcttcctt aatagaaact
     1321 tcaacaatac cttctgtttc ttttaaaggc acatctataa taggatcttt aatttctcct
     1381 accaattctt tcacttgatc taccgttaac aaagcaaatc ccccttaatc attttaagac
     1441 atctacatca tagcaaataa caatttttgc ttagtctatc actaaaagta taaaactttt
     1501 tattaaataa aaaaagcagt tagcctatta ttttaaatag accaactact ataattattt
     1561 taaaatttcc ttctattact ttctatttct ttaggttctc ttataaaaag tgctatcaca
     1621 taagctgcca taacaagcac agctacaaaa gcaaatgaga tatccacccc tatagcaatg
     1681 gattgctgtt taatttcagt tgcagttaaa tgcgatggcg atgtagataa aaatattttt
     1741 gcaccaaaac tcattaatgt aaccattaaa gctgtgccta gtgaaccagc cattacacga
     1801 ccaaagttca taatagccgt gccatgtgag atttcttcat ttctcaaaga attaattcct
     1861 gtagtattta tcggcatcat gagtaaagaa actgaaaaca ttctaattgc atatacgact
     1921 attaaatacg tataagatgt gtctgtatgc aagaaacaca acataattgt agtaattgtt
     1981 aaaattgtaa aaccagtata aatcaatggt ctaggaccat acttatcata aaatttacct
     2041 gtaaaaactg acataatacc atttattatt gcaccaggca tgataacaag tcctgataac
     2101 aaggcagata aagataaact gttttgaaca tatagcggta taagcagcgc aggtccaacc
     2161 attgacatca ttataatcat tgaactaatc gtacataatg caaatgttct atttttaaaa
     2221 acccttaaat ttaaaagtgc attagtaata ttaaattgtc tacgtataaa taaatagata
     2281 ataaccatac ttattatcaa cgcacctatt actattggac ttgtaaatcc taaatctcct
     2341 gcactactaa atgcgtatag cattaacccg aatccaatag ttgaatacat tactgagcgc
     2401 ttatctaatt tcgtatattt cacttcattg taacttgaga ttgaaactaa gccgaaaaca
     2461 aaggcaacta aagcaattcc tacaattata ataaatggta ctcgccaact cgcttgatca
     2521 ataattaatc ctgtaactgt agggcctatt gctggtgcaa attgaattac taaacctgct
     2581 agtcccattg caaaacctcg atgttcttta ctgaacaatg taaataacgt aaattgcatt
     2641 aagggcataa gtacgcctgc acccatcgct tgaattacac gtgctaacat taaaactcca
     2701 aaattcggag ctaaggctgc aacaatagaa cctaataaaa atgtccccat cgccgctaag
     2761 tataaaggtc tagttttaat tctatccatt agatatgccg tcaaaggtat catgacgcca
     2821 ttaacaagca taaacccagt aactagccat tgcgatgtac tttcattaat atttaattct
     2881 ctcattatac taggtaacgc tgtatttaat aatgtttgat ttaaaatagc tacaaacgca
     2941 ctaatcaaca taacagcaac tatgaagttt cgtctccggg ttgttaattc aactttagcc
     3001 atcataattc ctccttttta aaacagatat acacttgtcc ctttaataca tatagccatt
     3061 aacataatgg gagtagtact caaatgataa agaagcacta cataataaat cattattggt
     3121 tcttacacat taggcacagc taatgtgtac ttaaaaaact aacaatataa cattgtaggg
     3181 cctaataatt tattttattt ctcagactca tacaatggtt atttaactta tttaccacaa
     3241 attgagcact aagactcact attatcgatt cttctattga acaaaaagaa gttggaacac
     3301 atatgtgccc tcaacttcag atatctattt tctattattt aaatttttac tttcagggta
     3361 aaattgaagc ttacaaacat ataagaaccc tataataacc atgatgaccc aaaaatcgat
     3421 attgatatac acatagttca gatgaataat aaaataaaat aacgttgttg caactgtgta
     3481 agaaatcaaa tgaaaagtcg cgcctctaaa atatgcattt tcaggcaaca tttttctaaa
     3541 ataatccatt aaatgctcga ctgcaaatat gatgaagtac ccaaagatta tataactgcc
     3601 ataatataat aggttatcgt acatacttct attatatgta aagtcaccca acctaaaata
     3661 aataagtatt ctacttaatc catataaacc aaaccctaga aatgttaaaa aaatcgcacc
     3721 agaaagtaca aaaataaaga gaacaaccat tgtagttaac aagttataaa acttatgttt
     3781 aagataattc acaatcattg gcctccttat taataaaata ttgaacgatt tatctagtat
     3841 ctaggaaatt tattatttct tactttaatt acactacatc aattttaatc ataattctat
     3901 tcttttgatt gagatgactg ttctttaaaa aatacgaccc ataaaacaat aagcccaatg
     3961 ctaattacag caacagcact taacacatta tgtaagtttg gaccaaagat tcctgattgt
     4021 atcgcataaa gatatcccat tattgttgaa cctattgatg ccccaagatt tttagttaat
     4081 ccataaaatg acatcatttt cttcatttgt aatgggctag tttcctcttg gacaattaca
     4141 ctatctttcg tataaatata tccaaaactt aatcctgcca aaatcaacac aaatgctata
     4201 agtaccggca atttaatacc aaatgaaatg ataatactac ttactagtag caatgtaaat
     4261 gatagtaagt aaataacttt ccttgatagt tttgcttcaa ttcgatgtaa attaaaattc
     4321 aatgtaatcc aagctaaaga taaaggaaaa ataaccaatc cactttgcaa tggagataat
     4381 cctagttgtt cttgaaggta gactggaata tataaattga atcccattaa acaaatagct
     4441 gttagaaggt cagttataaa aactaaagta atcgaacgat taaattcaac cacaggtaaa
     4501 aatggtgaag aaacatgttt ttctacttta aataaacaca ttgccacaac gatagctaag
     4561 ataaaactca agaaatttaa aagtaacagc tgttggttta ataaagcaaa cataatcaat
     4621 ccaataaata cataaaatag cgtgagccct ttagtatcaa attttgattt agcaactgtt
     4681 tcttcctctg gaaaatgaaa tgtccatata actaatataa tggcaatgat cgcaatcggt
     4741 atatttatgt agaataacca atgccatgtt gcaaattcta atataccacc acctagaagt
     4801 ggcccaatga tactcgaaat accccaaaca ctgccaacta tgcccataat tttatatcgt
     4861 aacggaattt caaatgctaa ctttggaaca atttgcgata atgacatcaa tacacctgat
     4921 ccaaaacctt gtatcacgcg tgatataatt agcatagtaa aattagtact taaaccacac
     4981 ataaaactac ctatactaaa taaaagtaat cctgcaattg ccacataaat aattttaaat
     5041 ctcgaaagta attcgcccac tataggattg gcaattacta atgcgataaa atacgctgtg
     5101 aaaattaatg aaattaaatt tcctgcattt aaatcttgtt taattgtagg taatgcaagc
     5161 gaaataatag atgattcaat cgctgacata aacataatta atattaaagc tataacagtt
     5221 atgattgact ttaatcgcat gaaatacctc cttctatata tttatttata agattttatt
     5281 tacaaataat tggttttcat atgtataaac acttttgact ttcaaatctt aacgataatt
     5341 ctattacaat acaatcccct attagaatga tttatgtaat aaaaaaagcg gagtttcccc
     5401 cagcttttct aaacgactac ataaaatata agattgcaat taaatgcaat agtgatgcta
     5461 ttacaataaa aatatgccaa atcatatgaa aatatggtct attcttttgt gcataaaacc
     5521 atgcaccaat tgtataagac acaccaccta agaaaatgaa taatatgaat atccatgatg
     5581 tgcgaataaa aataattggt aacaagataa tacctaccca gcccatcact aaatagacaa
     5641 ttaagcttaa cttttggtta actttgacag ctatggattt atataaaata ccccaaagtg
     5701 ttgttcccca taataaaata aatactgtcc aacctatcca accacctaca actgttaaca
     5761 aaataggcgt atatgtacca gaaattgcta cgtaaatcat actgtggtcg ataatcctta
     5821 aaatatattt atgaggtgtt tcattttgca tagaatgata aatagtagat gaaataaaca
     5881 tcataaagat agaaataaca taaatagaca tactcacaga cgcaagcgta ccatgtgcaa
     5941 tataactgtg cacagccgca tagggtaaca ctaataatgt taacaaagct gcaacaccat
     6001 gagatgccgc attaccaatt tcttctccaa atgataaagg gatgatatcc ttaaatgttt
     6061 ctacgattgt gtcattactt tttttagttg atttggacat catagtcacc gcctatcatt
     6121 aaattaaacc cattctacgt aaatcttctg ttgcagttgc aacactatct ttaccttctt
     6181 tatttttaag aggtgaaaat tcttcttctc taggaacttg taacgtgaca aaactagttc
     6241 cataagtaaa tatgtcaaaa tagaacaatt cgaattttaa agttggttgt tcaataaccc
     6301 caaagttctc atctaattgt ttcaaattct taatagctaa atgataaggt aactcacgat
     6361 ttacagcgtt taaaataaaa ccaagtttaa atgcatgaat accgatatta gcattgttaa
     6421 attgatttgc aacttctgga tctaattctg aatactccag tactgtatct ttacaatcga
     6481 cattaactaa acgtccaaca ctttctcctg gttttggttg aatcgtttta gaagtaatat
     6541 cgtaatcatg ttctaccgta aatcctgcaa atagtggatc caacactttt actaaaacat
     6601 tgtcgatatt attcaagaaa atatacttaa cgccattatt agacatttct tctaagtatc
     6661 ctgccttgtc taaagattta aacacgccac cattaccatt cggcgtttcc ataatacgac
     6721 cctgttgatt taaaattaac ttcccttctt cactaagcgc cacaatatta tcttgtttaa
     6781 agaaatggat tgcttcttga tcatatccaa aataactatg tgcttcgaaa taagctaaag
     6841 tttcttcatg attgatatca cttgtcataa tataccattg aatcgtatgt ccagattgat
     6901 gattcaatgt ttttaattgg ttagcttgaa gttcaaataa acttacacct tcaatttcaa
     6961 acgaaccttt agggcccttg taaccaagtc ttgttccttg accacctgcc atcaaaagta
     7021 ctgcaaattg tccttcttta atagcttgaa gtccttgttc ctctaaacgc ttaatctcat
     7081 catcactcat ttgtgattta atatcatatt tcacttcaga aactgcagat acatcatcta
     7141 ttgttttctt attgatgtat aaatcgttat ataatttggc aataaaatct aaatctaatg
     7201 acgctacctt ctcttcaagt gcttcttttt cattgttact cattattttt tcatattcac
     7261 acaaatgatc ttgtttatat ttagctaatt gatttttatc tagcatttta tgcctcctgc
     7321 tgagtttgta acctttaata atttattttc tataaaaact tagtattcca gttgcttatt
     7381 atatcattga tgaaaggctg aaataaaaca taaactgttc cgaccattaa cgctgtagct
     7441 aaagataagt ctacaagtcc acctgtttta aattgaatcg gtgtcttcac attaaacggt
     7501 aacggataaa ataatttcac gcctcttggt gttatcatat ctagtataac atgtgacact
     7561 aacccaatta taattgctgc catataataa ttaggcgtct gaataatttg caataaaaat
     7621 gctatgattg cgataaataa tattgaatgc gtaaaggttc gatgtccaaa gatcaatctc
     7681 acaaaaaaac taattacctt aaaccttctg ccaatcttac tttgagtgtg acatatatcg
     7741 ggtaataagc tagctagagt tgctagaatg ataaccgtaa ccgacgaaaa aatatccgtt
     7801 tgaaaatatt gtgttgttag cgctccaacg agcatgccgc atgaagcatg tgttttacct
     7861 gtcatatttg ttctccttta atactcacat tttaccacat ccctaacaaa aacacgaaca
     7921 tattttcggg ttaaaattca ttagtatgac acaatttaaa aaagtatcac ataactcttg
     7981 aaaacgatta caaaatcgtt tatgatgtat ttacaaaata tttaaaggat gtgtttgaat
     8041 aatggcaatg acagtaaaga aagataataa tgaagtgcgt attcaatgga gagttgctga
     8101 tatcaaaatt cctacaagtg aaattaaaaa tattacacaa gaccaagata ttcatgcagt
     8161 tcctaaatta gacagcaaag atgtatctag aatcggctca acgtttggta aaacgaatcg
     8221 cgttattatc gatactgaag accacgaata cattatttat actcaaaatg atcaaaaagt
     8281 ttacaatgaa ttaactaaat aaattgtata aaaaaatcat tcatggtgag ggcttcatga
     8341 atgatttttt taattgattc aacacccagc aaaaacaaat acaaaaggac aactgttctc
     8401 ataattttaa cagttgtcct ttttcacata tatttataac aaaagatgtg ccatcaaaga
     8461 aataattggt agtgtaatga ttgttctaat caagaaaatc ataaacaatt tgccgatact
     8521 tacaggaatc ttcgaaccaa gtatgacgcc acctacttca gacaagtata ttaactgcga
     8581 tatactaagt gccccaataa caaaacgagt tatatcattt tgtacacctt caattaatat
     8641 agaaggtaaa aacatatcgg caaaaccgat aataatcgtt tgagaagcct gtgccgcttc
     8701 aggtatttgc attaactcta aaaatggaac aaaaggttta cccaatatga caaaaaaggg
     8761 cgtgtagttc gcaataatgg tagcaatagt accaatactc atcactacag gcaaaataac
     8821 aaaccacata tcaatgactg tttttaatcc tgacttaaaa aagtcaataa cgcccggtgc
     8881 tttaatacca acttctgttg cagtatcaaa gccatgtctc aatgccgtct ttccttctgg
     8941 caatgcctca gtacgcgcac tttcaggtac ctccttagca tactcatcag gaattttatt
     9001 taaaggccaa attcttggca taatgactgc tgcaacgagg caggatacta tcactgataa
     9061 atagaaagca aaaaattgat tttgcatgtg cactgtttca gcaactacaa ttgcaaaggt
     9121 gatagaaact acactaaatg tcgttgaaat aactgttgcc tcacgacgag aataatatcc
     9181 ttcaccatat tgtctacttg taattaagac cccaacagtt ccgtctccaa taaatgatgc
     9241 taaattatct accgtcgaac gtcctggcaa tgtaaataaa ggtctcataa ccggtctaaa
     9301 aataggaccc aacatctcta acaaaccgta ttccattaat agcggtaaaa atagagctgc
     9361 aaataagaat actgccacta atgttggcaa taaacttgag aacaataatc cacccgtttc
     9421 atctgagtaa ataacctttg aaccaattcg taaaaatgtc atccatgcaa aaacaactgc
     9481 taatattcgt aaaattaacc aaccaattct aacgttaaaa gcattgttca ttagcccgtc
     9541 aggtttcaat ttatctttta aaatagttga acaaatcaga gttatgatac ccgataaagt
     9601 aattatcgtc acaattaaaa atggcattac gccacctaat acatctttaa gcacgcctgc
     9661 taaaaatgcc acgggcaacg ttgtttgctt ctgtccatct tgttcgactg gaattggcac
     9721 taaaaataat aagataccaa ttaaagacat cgtaataaac ttaagtctcc caataactat
     9781 ctctttcctt gaaaagctat ccataaaatc aatccatttc tctatgtatt cgttttaagt
     9841 atatacagaa ttctattcag ttaacaaaca tattccttat cattctatct ttcaaaatgt
     9901 ttatgtatgc aaaataatga ataattacag ttattaaata tacgctattt cttgtaattt
     9961 ttcaagatga attcaaaaaa ggttaagtac aattactgat ttcgtactta acctttttta
    10021 aactctaatc atatgttagt tatttcattc ttcgtaataa tattaagaag tatggtgcac
    10081 cgataattgc aatgataacc ccaacaggaa tatccagtgg cggatgaatg ccacgggcta
    10141 aaccatctcc aaatgttaac aatatagcac caattaaccc cgacatgata ataacgtgta
    10201 atgttttatt tcctattaat tgtctcgcaa tatgaggtgc aattaatcct aaaaagctaa
    10261 taccaccgac aactgaaatt gcggatcctg ctaatattac tgctaaaatt aacaatagca
    10321 ttttaatagt tttaactttt aaaccgagtg cggttgcaac agcatcacct agattcaata
    10381 catctaattg ataactcaat aaaatgatga ttggtatcgt tattaaaaac caaggtaata
    10441 tagtataaat attcgacata tcatgtccat atagactacc tgtcaaccaa acaagcgctt
    10501 tgtttgcttc cagtggattt ctgattaata agaactgcac aatcgccgta catattgcgc
    10561 ctattgctaa accaattaag gcaagctttg aacctttaac atcatatttt gaaattaaaa
    10621 atgataaaaa taaacttact gcaaaggcac ctaagaatga acctataggt aatacaaaca
    10681 atggtgctgt tggaaaggtc ataataataa tcacagcagc taaactggca cctttagaaa
    10741 tacctataac atcaggtgag gctaacgggt ttcttattac agcttgtata attgcacctg
    10801 aaatagccaa gctactgccg ataataatac caagtaatgt tctaggtata cgatactcat
    10861 ttaaaataaa atcatcttgt gtaaagattc ccttaatagc atcaatcgga tgaatcatga
    10921 cagaccctac acataaactt atgaatatac tcacaattaa aaggattgtg attaaactat
    10981 aacgacgtat aattttcgtt gtcatcatat tcttttcacc cctttaatcg ttataaataa
    11041 gaagtaaagt gcacctacga atgatgtaac aatccctact ggtgattcat aaggatatgt
    11101 aattaaacga cttaatacat ctgatagtag taacaaatct gcacctataa taaatgttaa
    11161 tggaatcatg actaaataat tcttactcac atagcgtttg actatatgcg gtacgattaa
    11221 gccaacaaag ccaattggtc ctgccactga taccgacata cctgtaagaa taatgactaa
    11281 tagtccaatg ataattctaa ctttatttat attttgacct aatcccttag caatgtcatc
    11341 gcctagttcc attatcgtta gttgacgtcc aataaaaatc gttacaatta aagcaccaat
    11401 aagccatggt agaatggtta aaatttcatc ccacttcata ctggataatg aaccgacaag
    11461 ccaaaacatc acttgttcgt ttgaattctc attcaaaata ataatacctt cggtcatact
    11521 actaaaaaac aaatggatgg ccataccagc caatgctaat ttaatgggtg tcataccttt
    11581 cgtagcacct gaaagtgtat aaacagttaa accacctata aatgcaccta tcacacctaa
    11641 atataatgca taatattcta atgatggaat gataatcgtc acaaatacaa tgaaaaaaga
    11701 tgcaccagaa ctgacaccaa aaattttagg tgatgctagc ggattgcgtg tcattgcttg
    11761 cattaacaaa ccagataccg ctaatgcacc gccaataaat aaccctgcaa tcatccttgg
    11821 catacgtaca ttatgcaata aaaacgttgc tttcgtatct gtatgaccag taacatagtg
    11881 aacgatatcg ctaaaattaa ttttcgaaga accaatcgcc atatttaaat atatacaaat
    11941 aaaaagaaag cacaaactca ctatatacgt gagtgttgtg cgtctttttc ttttatgatc
    12001 tatggcagat tggctactta tttcttttat agccatttac ttccacctta cttttgttct
    12061 ttttttgata attcaacaag ttctttagcc atttcttcag aagaaattaa gccacgagat
    12121 cttgcccaaa catcacggtc aacaatatcc acgcgattat ttttaactgc attcaatttt
    12181 ttccatgttg catcttcttg taatttcttg aattcagcag aatctttttt agcgtgatca
    12241 gtcataatga tcatacgctc tggatttaaa tcagctaaat gttcagtgtc taattgtaag
    12301 taaggtcctt tcaaatattt acttaaacct tttgttacat cgtcacttaa tgcattttta
    12361 aatcctagtt cgtttaaaaa ttgtccaaca tatgaatagt ttggatgtgc taataaacca
    12421 gctttagcaa ctactgctgg aagcactttt tgatttctat caaatttaat ttcatcttta
    12481 tacttattga ttaatttatc atgctcagca agacgttttt cgccttcttt ttctttattt
    12541 aaagctttag caattgtttt gaacgaatta atgttttgtt tgtagtctcc atcaaaactc
    12601 tttaatgata atgttggtgc aattttgttt aattctttat taataccttt atgtctactg
    12661 ctatcagcga taattaaatc cggttttaat ttactaattt cttctaagtt tggctgttta
    12721 cgtgtaccta cagaagtata atccccaatt ttttctctaa ctggtttaat gatacgtttt
    12781 ttcttaccat catcagcaat accaactgga ttaacgtcta atgctgctaa tgcatctgca
    12841 aatgagtact ctaatacaac gatacgtttt gcatctttag gtacttttac tgtaccattt
    12901 tcatctttta ccgaaatagt atctttagtt gatgattctt ttttacttga attatccgta
    12961 ttaccacaag ctgcaactaa aagtaaggca actattaatc ccaatatact aaaagttttt
    13021 agacctctca tcgtt
//
LOCUS       DAMZKC010000069        12904 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.069, whole genome shotgun sequence.
ACCESSION   DAMZKC010000069 DAMZKC010000000
VERSION     DAMZKC010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12904
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.069"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            71..1900
                     /locus_tag="POB55_001442"
     CDS             71..1900
                     /locus_tag="POB55_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HDI6500408.1"
                     /translation="MFNQFKRLIIGQPKKNRELKDEKISKFKGLAILSSDALSSVAYG
                     PEQILITLSVVGAVATWYTLPIAGAVLILLAALIMSYRQIIYAYPKGGGAYMVSKTNL
                     GEKWGLLAGGSLLVDYILTVAVSISSGADAFVAAFPSLYGHKVLIACLLVLFILILNL
                     RGLTESATVLSYPVYLFIIGLVILIFIGTFRVATGDIQPHMHASVGTAVPGVTLFLLL
                     KAFSSGASSLTGVEAISNAVTNFREPSANNAVKTLIAMGSILAFLLVGIVGLAYVYGI
                     MPQTETTVLSQLAMQIFGDNAAFYFVQATTVMILVLAANTGFTAFPMLAASMSKDKYM
                     PRMFTVRGDRLGYSNSIIILGVLAIILIIVFDGMTEDLIPLYAVGVFIPFTLAQFGMV
                     IKWIHERPKNWLSKLSVNLLGGIVTFIVFMILLITKFSQVWPILIFLPFVVIFFLKIN
                     KHYRDIAEQLRSDIDVLNVDVVDRNLAIVPITSITTAVDKSIYYAQMLANNDVIAVHV
                     SFGDEDEKAFQEKWKRHFPDVRLVILHSEYRSIIRPISRFIDKINRKANDQNYMITVV
                     IPEFITKKRWHNLLHNQTSLRMKLYLIYQKNVNVCTIPFKLKK"
     gene            complement(2040..4118)
                     /locus_tag="POB55_001443"
     CDS             complement(2040..4118)
                     /locus_tag="POB55_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HDI6500409.1"
                     /translation="MALLEAFLIFIFAVIISSVINNRFPQIPTAFIQIALGVVIFIIP
                     IQVDFQFNSEVFMFAVIAPLLFVEGTHVSRTKLLEYRKPILLMSMALVFATVVGVGYF
                     IHWIWPALPMPAAFAIAAILCPTDAVAVSAITRGKLLPKGSMTILEGESLLNDAAGII
                     SFKIAVTALVTGTFSLFQAVEQFIISTILGVLIGAIIGFVVVRIRIDLTANKGLKDNN
                     TLTFIQLLTPFVVYFLAEEVHASGIIAVVIAGLIHGLERDRLIRAQTELQMNYHQIWN
                     TFSYALNGFVFVVLGFMIPTVVIDIFQTEPDNFSFLIVITILIAIAIYACRFVWVYFW
                     YKDFYFPKNIQSYLDEEHDSHETPPSRVRYAFIMTMCGIHGTISLSMALTLPFIITKG
                     QAFEYRNDLLFIASFMVLISLILAQIVLPLITPSAEDTTFKGMTYQSAKIFIVQKVIQ
                     HFKNESKKDKNDTNYRPVLNQYYGELLFLLNSEPDNQNTKELKRLEDIAKVIETSTLE
                     RLIDKGKATYQDINNYRNIVELTETHRTASFVEKFANFFKMLWLRLKAMKHYKALNKE
                     SKKQEFENSFKDVQKIMRIVNHNIILRLKEEQNSTNVLEVSLVINHYYDMSRSLKWRA
                     QRRKERQENSNQIIPQAMFHNHKLEALYLQRHLLDELIRKNKINNIVAAQIRENINYN
                     EIVLSLQSKH"
     gene            complement(4256..5665)
                     /locus_tag="POB55_001444"
     CDS             complement(4256..5665)
                     /locus_tag="POB55_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDI6500410.1"
                     /translation="MARKLHRELNNRHIQLIAIGGAIGTGLFLGSGQTISLTGPSLLF
                     TYMIIGVVLFAFMRALGELLLSNTRFNSFVDIANEYLGPFGGFVIGWTYWLCWIVSSM
                     SDLTAMGQYFAFWYPQVPNWITVLFIVLILISFNLLGARLFGELEFWFSIIKVVTIIA
                     MVIVGLVLIFFSFKTHYGHASFTNLISHGGMFPGGTFGFLMSFQIAVYSFIGIELIGV
                     TAGETKDPEKTLPKAINNVPIRILLFYIGGLLVIMSVIPWNDIDPNSSPFVKLFTLIG
                     VPFAAGVVNFVVLTAAASATNSGIYSNSRILFGLSQQGLGPKVLNKTNSHGVPYLSML
                     VSSIALLIAALLNYIFPNAIQLFIYVTTLSTVLFLVVWAMIIVAYLMYLKKHPEAHKN
                     SKFKLIGGKPIAYIILAFFFFVFILLFFSDETRAAIYISPFWFIFLFFFYKKYKTNAE
                     KLAYEQRQNDSGHFRYDNQ"
     gene            5951..6757
                     /locus_tag="POB55_001445"
     CDS             5951..6757
                     /locus_tag="POB55_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDI6500411.1"
                     /translation="MKIGTIADLHIDRHNKKTSEDYLEALVEIVKYKKLDILLIAGDI
                     SNHYQLTHQFITQLTKQLDIPVKFVPGNHDLWEVESMTTQDIWNNYKSMSQCLVGKPF
                     IVNEEWAIIGHTGWYDYSFAAQRFSLEHLQKGKHYGATWQDKERISWGISDQNLSKIA
                     AEQVKKDILEVGNRRVILVTHVVTHPDFIVPMPHRIFDFYNAFIGTSDFNPLYAMFDI
                     PYSIMGHVHFRKSVIDDGRCYLCPCLGYPRQWRSEDIYQEINETIQIIEI"
     gene            complement(6820..7476)
                     /locus_tag="POB55_001446"
     CDS             complement(6820..7476)
                     /locus_tag="POB55_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HDI6500412.1"
                     /translation="MNRKILPVKPISQLFPIPMVMGCEGVSAAMMLQYNNQHIPATEI
                     MRHWPTHPNNPHKGYVGHHLFIKLGNYHQTIFPEAYVPFLQKYNPNIVDGTGKSLEDL
                     KGIIDQGQPVLIYHTNLGSKPLLRVFRFDNKPAKQVSNIHVTLLIGYDDYYYYYIDPL
                     WSHIRRGLVLPAIIPNRKQIIKIRKEKMEYSFNSPGRKCIYVQPHSYTIENQQQNKHT
                     "
     gene            7589..8062
                     /locus_tag="POB55_001447"
     CDS             7589..8062
                     /locus_tag="POB55_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDI6500413.1"
                     /translation="MEYQLQQLASLTLVGIKETYENGRQAQQHIAGFWQRCYQEGVIA
                     DLQLKNNGDLAGILGLCISELDGKMSYMIAVTGDNSADIEKYDVITLASSKYMVFEAQ
                     GAVPKAVQQKMEEVHHYIHQYQADTVKSAPFFELYQDGDTTSEKYITEIWMPVKG"
     gene            complement(8185..9396)
                     /locus_tag="POB55_001448"
     CDS             complement(8185..9396)
                     /locus_tag="POB55_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HDI6500414.1"
                     /translation="MQSTKTKTKHFSFLLLITLGVMTAFGPLTIDMYVPSLPKVQGDF
                     GSTTSEIQLTLSFTMIGLALGQFIFGPLSDAFGRKRIAVSILIIFILVSGLSMFVDQL
                     PLFLTLRFIQGLTGGGVIVIAKASAGDKFSGNALAKFLASLMVVNGIITILAPLAGGL
                     ALSVATWRSIFTILTIVALIILIGVASQLPKTSKDELKQVNFSSVIKDFGSLLKKPAF
                     IIPMLLQGLTYVMLFSYSSASPFITQKLYNMTPQQFSIMFAVNGVGLIIVSQVVALLV
                     EKLHRHILLIILTIIQVVGVALIILTLTFHLPLWVLLIAFFLNVCPVTSIGPLGFTMA
                     MEERTGGSGNASSLLGLFQFILGGAVAPLVGLKGEFNTSPYMIIIFITAILLVSLQII
                     YFKMIKKQHVA"
     gene            complement(9578..10237)
                     /locus_tag="POB55_001449"
     CDS             complement(9578..10237)
                     /locus_tag="POB55_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500415.1"
                     /translation="MKRTDKYRDSYQYDNQNQNHRRQSEDASYRQQYAKGDPEEHPER
                     YYNGRDYRREQILEEENEKSRRSKKWLYIIIAILLIIVAIFVTCALLNNDSDKVSNDP
                     KVSQNYKKQVENQDGQINQQVDNAKENIKNNQKTDDIIKNLQNQIDNLKQQEQNKADS
                     KLTQFYQDQINKLTEANNALKNNASQGKIESMLNDINTKFDSIKSKLESLFKDENGGA
                     N"
     gene            complement(10297..11439)
                     /locus_tag="POB55_001450"
     CDS             complement(10297..11439)
                     /locus_tag="POB55_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDI6500416.1"
                     /translation="MQLQKIVIAPDSFKESMTAQQVGNIIKQAFTNVYGNTLHYDIIP
                     MADGGEGTTDALMHATGATKYTVIVNDPLMRPIEACYARADEQQIAIIEMAAASGLDL
                     LEKEERNPLYTSSYGTGELIKDALNHGAKTIILGIGGSATNDGGTGMLSALGVQFTDV
                     NGDLLQMNGANLAHIAQIDITNLDSRLKEVTFKVACDVSNPLLGENGATYIYGPQKGA
                     DAKMIPKLDFAMSHYHDKIKMCTGKSVNQIPGSGAAGGMGAALLAFCETTLTKGIDVV
                     FDITDFHQRIKDADLVITGEGRMDYQTIFGKTPVGVALAAKQYHIPVIAICGSLGENY
                     QHVYDFGIDSAYSIISSPSTLEDVLQNSEQNLLNTATDIARILKLQ"
     gene            11697..12083
                     /gene="gtxA"
                     /locus_tag="POB55_001451"
     CDS             11697..12083
                     /gene="gtxA"
                     /locus_tag="POB55_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase GtxA"
                     /protein_id="HDI6500417.1"
                     /translation="MKLTQTHAEILKFIIVGGINTLNYYVVYLLLLKLLHIEYMISHI
                     TGFLVAFVISYYLNCYFVYRVKPTWRKFISFPITQIVNVSLQTVLLYVFVSWLNLPAE
                     IAPFAGLVITIPITFVLSKWILKDSN"
     gene            12217..12324
                     /locus_tag="POB55_001452"
     CDS             12217..12324
                     /locus_tag="POB55_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HDI6500418.1"
                     /translation="MFNLLIDIMTSALSGCLVAFFAHWLRTRNNKKGDK"
BASE COUNT         4317 a         2010 c         2330 g         4247 t
ORIGIN      
        1 tctctatact ttttggaaag ataggcgtat agtgatatcc atcaaaaact ataaaaagga
       61 ataggagatt atgttcaatc aatttaaaag acttattata gggcaaccta aaaaaaacag
      121 agaactaaaa gacgaaaaga tatcgaagtt taaagggtta gcgattcttt cgtcggatgc
      181 attatcttca gtggcttacg gtcccgaaca aatactgatt acattatcag tagtgggtgc
      241 agttgcaact tggtatactt taccgattgc aggtgctgtt ctgatcttat tagctgcttt
      301 aattatgtct tataggcaaa ttatttatgc atatcctaaa ggtggcggcg cgtatatggt
      361 gtccaagacc aatttaggag aaaaatgggg attgcttgca ggaggctcat tattagtcga
      421 ttacatactg actgtcgctg ttagtatatc atctggtgct gatgcatttg ttgctgcatt
      481 cccaagttta tatgggcata aagtactgat tgcatgttta cttgtacttt ttattttaat
      541 attaaattta cgtggtttaa ctgaatctgc caccgtatta tcctatccag tatatctttt
      601 cattataggg ttagtgatat taatatttat aggtactttc cgtgtggcga caggagatat
      661 tcaaccacat atgcatgcat cagtaggaac tgcggttcct ggagtaacat tattcttgct
      721 attaaaagca ttttcatcag gggcttcatc attaacaggt gttgaagcta tttcaaatgc
      781 ggtaaccaat tttagagaac caagtgctaa taatgctgta aaaacgttaa ttgctatggg
      841 ttcaatttta gcgtttttat tagttggtat cgttggttta gcatatgttt atggaattat
      901 gccacaaacg gaaacgacag ttttatcaca attagctatg caaatatttg gtgataatgc
      961 tgccttctat tttgtacagg caacgaccgt gatgattttg gtattagcag ccaacactgg
     1021 atttacagct tttccgatgt tagcggcttc aatgtctaaa gataaatata tgcctagaat
     1081 gtttactgtt agaggtgatc gcttaggcta ctctaactcg attattattt taggtgtatt
     1141 agccattatt ttaattattg tatttgatgg tatgactgaa gatttaattc cattgtatgc
     1201 ggttggtgta ttcattccat ttacactagc acaattcggt atggttatta aatggattca
     1261 tgaacgtcct aagaattggt taagtaagtt gtctgtgaac ttactaggtg gtatcgtgac
     1321 atttattgtt tttatgattt tactcataac taaatttagc caagtatggc cgatacttat
     1381 tttcttgccg ttcgtggtga ttttcttctt gaaaattaac aaacattatc gagatatcgc
     1441 agaacaatta cgttctgaca ttgatgttct taatgtggac gtggtagatc gtaatttagc
     1501 aattgtgccg ataacaagta ttacaacagc agttgataag tctatttatt acgcacaaat
     1561 gcttgcgaat aatgatgtca tagcagtaca tgtatcattt ggagatgaag atgaaaaagc
     1621 attccaagag aaatggaaac gtcatttccc agatgtaaga ttagttattt tacattcgga
     1681 atatcgcagt attatacgac caatttcacg ttttatcgat aagattaatc gtaaagcaaa
     1741 cgatcaaaac tatatgatta cagtagtgat accagaattt attactaaga aacgttggca
     1801 taatttatta cacaatcaaa ctagtttgcg tatgaaattg tatttaattt atcaaaagaa
     1861 tgttaacgta tgtacaattc catttaaact taaaaaatag attctataat caaatcggat
     1921 tgatgaaagg ttatttactt ttcatcaatc cgattttttg atgttgaata taaaaacccc
     1981 gtacatatga gatgataatg actttgtctc atatgtacgg gggtattgtt atgtcttgct
     2041 taatgtttgg actgtaaaga caagacaatt tcgttgtaat tgatattttc tcgaatttga
     2101 gctgcaacga tattgttgat tttatttttg cgaattaatt catctaaaag atgacgttgt
     2161 aagtacaatg cttccaattt gtggttatgg aacatagctt gcggtatgat ttgattgctg
     2221 ttttcttgac gttcttttcg acgttgtgca cgccacttta atgagcgact catatcatag
     2281 taatgattaa tgactaagct aacctcaagt acatttgtac tattttgttc ttcttttaag
     2341 cgtaaaataa tattgtgatt cacaatacgc ataatttttt gaacatcttt gaatgaattt
     2401 tcaaattctt gcttcttaga ttctttattt aaggctttgt agtgcttcat cgctttaagt
     2461 ctaagccata acatcttgaa gaagtttgca aatttttcaa caaatgatgc agtacggtgt
     2521 gtctctgtta attcgacaat attgcggtaa ttattaatat cctgatatgt tgccttacct
     2581 ttatcaatta aacgctcaag tgtagatgtt tcgattactt ttgcaatatc ttctaaacgt
     2641 ttgagttctt ttgtattttg attatcaggt tctgaattta ataaaaataa caattctcca
     2701 tagtattggt ttaatactgg gcgataattt gtatcgtttt tgtctttttt actttcgttt
     2761 ttaaaatgct ggatcacttt ttgaacaatg aaaattttgg cagattgata agtcatacct
     2821 ttaaaagtag tatcttcggc agatggtgta attaaaggta aaacaatttg cgctaagatt
     2881 aaactaatta ataccatgaa agatgcaata aacaataaat cattacggta ttcgaatgct
     2941 tgtccttttg taatgataaa tggtaatgta agtgccattg aaagtgaaat tgtaccgtga
     3001 ataccacaca tggtcataat aaatgcgtaa cgcacacgag aaggtggtgt ttcatgtgaa
     3061 tcatgttcct cgtctagata agattgtata tttttcggga aataaaaatc tttgtaccag
     3121 aaataaaccc aaacaaatcg acatgcataa attgcaatag cgattaatat agtgatcact
     3181 attaaaaatg aaaagttgtc aggctctgtt tggaaaatat ctatgactac agtaggaatc
     3241 ataaaaccta atacaacaaa tacaaaacca tttaaggcat aactaaatgt gttccaaatt
     3301 tgatggtaat tcatttgtag ttccgtttgt gctctaatta aacggtcgcg ttctaaacca
     3361 tggattagac ctgcgattac aactgcaatg atacctgaag catgaacttc ttctgctaaa
     3421 aagtatacga caaaaggagt taataattga ataaaagtta aggtattgtt atcttttaaa
     3481 cctttattag cggttaagtc tatacgtatt ctaacgacaa cgaatccaat aattgcacca
     3541 ataagtacac ctagtattgt tgaaatgata aattgttcaa cagcttggaa taatgaaaag
     3601 gtaccagtta ctaatgcagt aacagcaatt ttaaatgaaa tgatacctgc tgcatcattg
     3661 agtaaagatt caccttctaa aatcgtcata gaacctttag gtaataattt tccgcgtgta
     3721 atagcagata ctgctactgc atctgtagga cataaaattg ctgctattgc aaaagcggct
     3781 ggcattggta aggcaggcca aatccaatgt ataaaatagc caacaccgac tacagttgca
     3841 aacactagtg ccattgacat taatagtata ggtttacgat attctaataa ttttgttcga
     3901 gagacgtggg taccttccac aaaaagtagt ggcgcgataa cggcaaacat aaatacttca
     3961 gaattgaatt ggaaatcaac ttgtattgga ataatgaaaa taacgacacc taatgcaatt
     4021 tgaataaagg cagtaggaat ttgcgggaat cgattattga taaccgaact aataatcaca
     4081 gcaaaaataa aaattaaaaa tgcttctaat agtgccatac aatactcctc aaaattttaa
     4141 tagttaatat tttatcactt ttaaggcata atgacataga tatattgata aaatgaagtt
     4201 attttcaaaa aaactctagt atcggttgaa ctgatactag agcgagatgt ttaaattatt
     4261 gattgtcata tctgaaatga ccgctgtcat tttgtcgttg ttcatatgcg agtttttcag
     4321 cattcgtttt gtatttttta taaaagaaaa ataaaaatat aaaccagaac ggcgaaatat
     4381 aaatagctgc tcttgtttcg tcactaaaga ataataaaat gaatacaaag aagaagaacg
     4441 ctagaataat gtaagcaata ggcttgccac caatcaactt aaatttactg tttttatgtg
     4501 cctcaggatg ctttttcaaa tacattagat aagcgacaat aatcattgcc caaacaacta
     4561 aaaacaacac agttgataac gttgtaacgt atatgaatag ttgaattgca ttagggaaaa
     4621 tgtagtttaa caaggctgct ataagtaatg caattgatga aactaacatt gataaataag
     4681 gcacgccatg actattcgtt ttatttaaaa ctttaggacc taacccttgt tgtgacagtc
     4741 cgaataagat acgactattc gaatagatac cactatttgt agcagaggcc gcggcagtta
     4801 gcacgacaaa gttaacgaca cctgctgcaa atggcacacc gattaatgta aagagtttaa
     4861 cgaaagggct actatttgga tcgatatcat tccaaggtat gactgacata attactaata
     4921 gaccaccgat atagaataat aaaatacgga taggtacatt attaattgct ttcggtaagg
     4981 ttttttcagg atctttcgtt tcaccagcag ttacacctat aagttcaata ccaatgaatg
     5041 aatatacagc aatttggaat gacattaaga aaccaaatgt tcctccaggg aacatgccac
     5101 cgtgactgat taagtttgtg aatgatgcat gtccataatg tgttttaaat gagaaaaaga
     5161 ttaatacaag accaacgata accatcgcaa taattgtgac aactttaata atagagaacc
     5221 aaaactccag ttcaccaaat aatctggcac ctaataagtt gaagctaatc aagattaaaa
     5281 caataaatag cacggtaatc caatttggga cttgtggata ccaaaatgca aagtattgtc
     5341 ccatcgcagt taggtctgac atacttgata caatccaaca taaccagtaa gtccagccaa
     5401 tgacaaagcc accaaaaggg cctaaatatt catttgcaat atcaacaaat gaattaaatc
     5461 ttgtattgct caacaacaat tcgcctaatg cgcgcataaa agcgaatagt acaaccccaa
     5521 taatcatgta tgtgaataac agtgatggac cagttaaaga tattgtttga cctgatccta
     5581 ggaataaccc agttccaatt gcgcccccaa ttgctatcaa ttggatgtgt ctgttattca
     5641 actctctatg caattttctt gccataattt tcctcctcag gataaagcaa ataaaaaagt
     5701 tgtgtgtaag cgttttctta attataaata ctaaagaaat ccatttaata aagcaattat
     5761 ttttttaaat ataagaaaaa acatttgata ggtaagggta tgataactaa aacttatcga
     5821 ttttttgaaa agatataagt ttttatgcaa aaatataaaa taatcatcta aatgaagcag
     5881 ttaactatat atattagata agctatggca taataaataa cattttaatt tgagtaaaga
     5941 aggtatgggt atgaagatag ggacaatcgc cgatttacat attgatcgtc ataataaaaa
     6001 aacatccgaa gattacttag aggcattggt tgaaatagtt aagtataaga aattggatat
     6061 attgttgatt gcgggagata tctcaaatca ttatcaattg acgcaccaat ttataacgca
     6121 attgacaaaa caattagata tacctgtgaa atttgttcct ggaaaccatg atttatggga
     6181 agttgaaagt atgactacgc aagacatttg gaataattat aagagcatgt cacagtgctt
     6241 ggtaggaaaa ccatttatag taaatgaaga atgggcaata ataggacata caggttggta
     6301 tgattatagc tttgcagcac aacgattttc attagaacat ttacaaaaag ggaaacatta
     6361 tggtgcgact tggcaagata aagaacgaat atcttggggc atatcagatc aaaatttatc
     6421 taaaatagcg gctgaacaag tgaagaaaga tatattagaa gtaggaaata gacgagtgat
     6481 tttagtcaca catgttgtga cgcaccctga tttcattgtt cctatgccgc atcgtatatt
     6541 cgatttttat aatgcattta ttgggacaag tgatttcaat cctttgtatg cgatgttcga
     6601 tataccatat agtattatgg gccatgttca ttttcgtaaa agtgtgatag atgatggcag
     6661 atgttatctc tgtccgtgtc taggctatcc aagacaatgg cgttcagaag atatttacca
     6721 ggaaataaat gagacgatac aaataataga aatttaaaat gcgcaaacct gacccagttt
     6781 gcgcatttta tgttttacac acgcgagtaa tgtgtttact tacgtgtgtt tattttgttg
     6841 ttgattttca attgtatatg aatgtggttg cacataaatg cactttcttc ctggtgaatt
     6901 aaagctgtat tccattttct ctttacgaat tttaataatt tgtttgcgat ttggaatgat
     6961 ggcaggtaaa actaggccac gacgaatatg actccaaagt ggatcgatat aatagtaata
     7021 gtaatcatcg tacccaatga gtaacgttac gtgaatattt gaaacttgtt tcgctggctt
     7081 gttatcaaag cggaaaacac gtagtaatgg tttagagcca agattagtat ggtatattaa
     7141 cacaggttga ccttgatcga taataccttt aagatcttct aaggatttac cagtgccgtc
     7201 tacgatatta ggattgtatt tttgtaaaaa tggtacatat gcttctggaa atatcgtttg
     7261 atgataattg ccaagcttaa tgaataagtg atgtccaaca taacctttat gtggattgtt
     7321 cggatgtgtc ggccaatgtc tcataatttc tgtagcaggg atatgttggt tattgtattg
     7381 caacatcatg gctgcggaaa caccttcaca ccccatgacc atagggatag gaaatagctg
     7441 actgataggt ttaactggta atatttttcg gttcataata tagtcctcgc attgattcaa
     7501 taaatattta atataattat atagcgtcaa tgcaaaatgt cctaaacata tgttttacat
     7561 gagtgaataa aattaatgga gtgataaaat ggaatatcaa ttacaacaac ttgcgtcgtt
     7621 aacgttagta ggtattaaag aaacgtatga aaatggacga caggctcagc aacatatagc
     7681 agggttttgg caaagatgtt atcaagaggg agtaattgcg gatttacagt taaaaaataa
     7741 tggtgattta gccgggatac ttggcttatg tatatctgaa ttagacggta agatgtcata
     7801 tatgattgca gttaccggag ataatagtgc tgatattgaa aaatatgatg tcataacatt
     7861 agcaagttca aagtatatgg tatttgaagc acagggcgca gtacctaaag cagttcaaca
     7921 aaaaatggaa gaggttcatc actacataca tcaatatcaa gcagatacgg taaaatcagc
     7981 accatttttt gagttgtatc aggatggtga tactacaagt gaaaaatata ttacagaaat
     8041 ttggatgcct gttaaagggt gattgaatag aaatgttctt gatatgaagg tgtaatttta
     8101 gttgaagatt cgatctcata attcataaag aagaaccctg acaaagatag atatctttgc
     8161 tagggttcta attatgttga agtgttatgc gacatgttgc tttttaatca ttttaaagta
     8221 aatgatttgt agactgacta atagaatggc tgtaatgaag ataataatca tatatggtga
     8281 tgtattaaat tcgcctttta agccaactaa tggtgcaaca gcgccaccta agataaattg
     8341 gaataagcca agtaaacttg atgcgttacc actgccacct gttcgttctt ccatagccat
     8401 tgtgaaacca agtggtccaa ttgacgtcac aggacacaca tttaagaaga atgcgatgag
     8461 taagacccaa agtggtaaat ggaatgtaag tgtcaggata attaaagcaa cacctaccac
     8521 ttgtataata gttaaaatga ttaataatat gtggcgatgt aatttttcta ctaataaagc
     8581 aacgacttga ctgacaatga ttaaacctac accgttaaca gcaaacatga tactaaattg
     8641 ttggggtgtc atattataca atttttgagt aataaatggc gatgcagatg aataactaaa
     8701 tagcattaca taagttaaac cttgtaatag cattggaata ataaatgctg gttttttcaa
     8761 aagacttcca aaatctttaa tgacgctact aaaattcacc tgctttaatt catctttaga
     8821 tgttttaggt aattgagaag cgacgccaat taaaatgatg agtgccacaa tagttaaaat
     8881 tgtgaaaata gaacgccatg ttgctacgga taaagctaat ccaccagcta atggtgcaag
     8941 aatagtgatg atgccattaa ctaccattaa agatgctaaa aatttagcga gtgcgttgcc
     9001 actaaattta tcaccagcag aggctttggc aatcacgatg acgccaccac cagttaaacc
     9061 ttgaataaat cgtaaagtta agaataatgg caattgatca acaaacatag acaaacctga
     9121 taccaaaatg aaaatgatca aaatggatac agcaatccgt ttgcgaccaa aagcatcgga
     9181 taaaggtcca aagataaatt ggcctagtgc aagaccaatc attgtgaatg ataatgttaa
     9241 ttgaatttct gatgtagtag aaccaaaatc accttgcact ttaggtaatg atggtacgta
     9301 catatctata gttagtgggc caaaagcggt catgacgcct aacgtaatta gcaataaaaa
     9361 tgaaaaatgc ttcgttttgg ttttcgttga ttgcatgatc agactccttt aaatgatata
     9421 attatataaa ttatatacgt tttgtgtgta tttgtatgta gttagaatac aagtttatat
     9481 aaaaaatccc tatactctta atgcatcatc acgaataacg tagtgataca gataaagtat
     9541 agggaattaa tgtttatcat caaagcaggt gtaataatta attagcgcca ccattttcat
     9601 ctttaaataa gctttctaat ttagatttaa tactgtcgaa ttttgtatta atatcattta
     9661 acatgctttc aattttacct tggcttgcat tgtttttaag tgcattattt gcctctgtca
     9721 atttgttgat ttggtcttga taaaattgag ttagcttaga atcagctttg ttttgttctt
     9781 gctgcttcaa gttgtcgatt tgattttgta aatttttaat aatgtcatca gttttttggt
     9841 tgtttttaat attttcttta gcattatcga cttgctggtt aatttggccg tcttgatttt
     9901 caacttgttt tttataattt tgagagactt tagggtcatt actaacttta tcgctatcat
     9961 tgttaagtaa ggcgcatgtg acaaaaatag cgacaataat taagagaata gcaataatga
    10021 tatataacca tttttttgaa cggcgggatt tctcgttttc ttcttcaaga atttgttctc
    10081 ttcgataatc tctaccgtta tagtatcgtt ccgggtgttc ttcaggatcg cctttagcat
    10141 attgttgtct atacgatgcg tcttcagatt gacgacgatg attttgattt tgattgtcgt
    10201 attgatatga atcacggtat ttatcagttc ttttcatggg tttcacctca ttcaagtttt
    10261 aactgcaaat aataaagctg atgatttact ttgacattat tgcaatttca gaatacgagc
    10321 aatgtcagtt gcagtgttta ataaattttg ttcgctattt tgtaggacat cttctaaagt
    10381 gctaggtgaa gagattatag aataggcact atcaataccg aaatcgtaaa catgttgata
    10441 attttcgcct agactgccac aaatcgcgat gacaggaata tgatattgtt ttgcagctaa
    10501 cgcaacgcct acgggtgttt taccaaagat ggtctgataa tccatgcgtc cttctccagt
    10561 aataacgagg tctgcatctt taattctttg atgaaaatct gtaatgtcaa agacgacatc
    10621 aatacctttt gttaaagttg tctcacaaaa cgctaataat gctgcgccca taccgccagc
    10681 tgcaccagaa cctggtattt gattaacgga ctttcctgtg cacattttta tcttatcatg
    10741 ataatgcgac attgcgaaat ccaactttgg tatcatcttt gcatcagcgc ctttttgagg
    10801 accataaata taggtagcac cattttcacc caataaagga tttgaaacat cacaggccac
    10861 tttaaaggtc acctctttta atcgcgaatc tagattggtt atatcgattt gtgcaatgtg
    10921 agcaagatta gcaccattca tttgtaataa gtccccgttt acatcagtaa actgaacgcc
    10981 cagtgcactt agcatacctg taccaccatc atttgttgca ctgccaccaa tccctaaaat
    11041 aatggtctta gcaccatgat ttaatgcatc tttaattagt tcaccggtac catatgatga
    11101 tgtgtataaa ggattacgtt cctctttttc taataaatcc aaacctgacg ctgccgccat
    11161 ttcaataatt gcaatttgtt gttcgtctgc gcgtgcataa catgcttcaa taggtcgcat
    11221 taaagggtca ttaacgatga ctgtatactt agtggcacct gttgcatgca ttaaagcatc
    11281 tgtggtacct tcaccaccat cagccatcgg aatgatatca taatgaaggg tattcccata
    11341 aacattagta aatgcctgtt ttataatatt gccaacttgc tgtgcggtca tactttcctt
    11401 aaatgagtca ggagcgatga caattttttg taattgcata gtagacatcc tctcattact
    11461 gttgtcgctc ttattgtata taaaattact agtgaactaa agtaatctct atagtgctaa
    11521 tgagtcacgc gataaatcag ttacattata atggcgtata aggatgagag gaaagagact
    11581 aaatttgctg tgaaagggta taaagattga aataaacata tttgaagtga agcggattgg
    11641 cacgcaattt cgagaacgta gttatgcata tattttgatg ttgaggagtt acacatatga
    11701 aactaacaca aacgcatgct gaaattttaa aatttatcat tgttggcggc attaatacgt
    11761 taaattatta tgttgtctat ttattgctgt taaaattatt acacattgaa tatatgatta
    11821 gtcatattac cggatttcta gtcgcttttg tgatttcgta ttatttgaat tgttattttg
    11881 tttacagggt aaaacctact tggagaaaat tcattagttt tccaattacg cagattgtca
    11941 acgtaagctt acaaacagtt ttattatatg tttttgtatc ctggctaaat ttgccagcgg
    12001 aaattgcgcc atttgcaggt ttagtgatta cgatacccat cacatttgta ttatctaaat
    12061 ggattttaaa agatagtaat taaagtagcg taaaccacta aaaaacgctt tactcgaact
    12121 ttaatattgc gtgaaatata ataaataaat gatataatat aatggtcgcc taacatctcg
    12181 ggcgttactt taacgcagag aggaggtgta tgacggatgt tcaatttatt aattgacatc
    12241 atgacttcag ctttaagcgg ctgtcttgtt gcgttttttg cacattggtt acgaacgcgc
    12301 aacaataaaa aaggtgacaa ataaccaaca cctaaataaa tcccctcact atggcagtag
    12361 tgaggggatt tggtgtattt agatgttcaa tttattatgt aattaattat agcattgatg
    12421 aacaaggtgt gcaatgtatt tttgtaatat tacaataaat gaatttaatt atattagaat
    12481 taagtatttc gaaaaatgat ttaatagttg agtcatagga tcattagata aagcgaataa
    12541 tcaattatag ggacattggg atacaaatca atgtactaga ctctacaagg ctatactcag
    12601 ggacccaaca tagagaaatt ggatccacag tttctacaga caatgcaagt tggcggagtc
    12661 ccaactaaga gaaattggat tcccaatttc tagagacaat gcaagttggg gtgggacgac
    12721 gaaataaatt ttgtgaaaat atcatttctg tcccactccc agaaccacta cataataaat
    12781 cattagtggc tctttatcat ttctgcccca taaccttgtg aatttttaaa agaaatgtgt
    12841 cacaaatcca cgcgacattg ataatcgtta tcaactaata tatgatgtac ttgtaataag
    12901 tatt
//
LOCUS       DAMZKC010000070        12749 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.070, whole genome shotgun sequence.
ACCESSION   DAMZKC010000070 DAMZKC010000000
VERSION     DAMZKC010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12749
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.070"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(56..307)
                     /locus_tag="POB55_001453"
     CDS             complement(56..307)
                     /locus_tag="POB55_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HDI6500419.1"
                     /translation="MANIKSAIKRVKTTEKAEARNISQKSAMRTAVKNAKTAVSNNAD
                     NKNELVSLAVKLVDKAAQSNLIHSNKADRIKSQLMTANK"
     gene            653..2476
                     /gene="lepA"
                     /locus_tag="POB55_001454"
     CDS             653..2476
                     /gene="lepA"
                     /locus_tag="POB55_001454"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HDI6500420.1"
                     /translation="MDNEQRLKRRENIRNFSIIAHIDHGKSTLADRILENTKSVETRD
                     MQDQLLDSMDLERERGITIKLNAVRLKYEAKDGNTYTFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNELELLPVINKIDLPAAEPERVKQEIEDMI
                     GLDQDDVVLASAKSNIGIEEILEKIVEVVPAPDGDPEAPLKALIFDSEYDPYRGVISS
                     IRIVDGVVKAGDKIRMMATGKEFEVTEVGINTPKQLPVDELTVGDVGYIIASIKNVDD
                     SRVGDTITLASRPASEPLQGYKKMNPMVYCGLFPIDNKNYNDLREALEKLQLNDASLE
                     FEPESSQALGFGYRTGFLGMLHMEIIQERIEREFGIELIATAPSVIYQCILRDGSEVT
                     VDNPAQMPDRDKIDKIFEPYVRATMMVPNDYVGAVMELCQRKRGQFINMDYLDDIRVN
                     IVYELPLAEVVFDFFDQLKSNTKGYASFDYEFIENKESNLVKMDILLNGDKVDALSFI
                     VHRDFAYERGKALVEKLKTLIPRQQFEVPVQAAIGQKIVARTNIKSMGKNVLAKCYGG
                     DISRKRKLLEKQKAGKAKMKAVGNVEIPQDAFLAVLKMDDE"
     gene            3100..4224
                     /locus_tag="POB55_001455"
     CDS             3100..4224
                     /locus_tag="POB55_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HDI6500421.1"
                     /translation="MTVQSAYIHIPFCVRICTYCDFNKYFIQNQPVDEYLDALITEMS
                     TAKYRNLKTMYVGGGTPTALSINQLERLLKAIRDTFTITGEYTFEANPDELTKEKVQL
                     LEKYGVNRISMGVQTFKPELLSVLGRTHNTEDIYTSVLNAKNAGIKSISLDLMYHLPK
                     QTIEDFEQSLDLALDMDIQHISSYGLILEPKTQFYNMYRKGLLKLPNEDLGADMYQLL
                     MSKIEQSPFHQYEISNFALDGHESEHNKVYWFNEEYYGFGAGASGYVDGVRYTNINPV
                     NHYIKAINKESKAILVSNKPSLTERMEEEMFLGLRLNEGVSSSRFKKKFDQSIESVFG
                     QTINNLKEKELIVEKNDAIALTKRGKVIGNEVFEAFLIND"
     gene            4325..5302
                     /gene="hrcA"
                     /locus_tag="POB55_001456"
     CDS             4325..5302
                     /gene="hrcA"
                     /locus_tag="POB55_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcription repressor HrcA"
                     /protein_id="HDI6500422.1"
                     /translation="MITDRQLSILNAIVEDYVDFGQPVGSKTLIERHNLNVSPATIRN
                     EMKQLEDLNYIEKTHSSSGRSPSQLGFRYYVNRLLEQTSHQKTNKLRRLNQLLVENQY
                     DVSSALTYFADELSNISQYTTLVVHPNHKQDIINNVHLIRANPNLVIMVIVFSSGHVE
                     HVHLASDIPFNNDKLNTISNFVTNKLTEFNQNLQDDIVSFVQSEQEEIFINKLLNTMN
                     NHISNQSNSIYMGGKVKLIDALNESNVSSIQPILQYIESNRIAELLQDISSPNINVKI
                     GNEIDDSLSDISIVTSQYHFDETLKGQIAVIGPTAMHYQNVIQLLNRIW"
     gene            5334..5960
                     /gene="grpE"
                     /locus_tag="POB55_001457"
     CDS             5334..5960
                     /gene="grpE"
                     /locus_tag="POB55_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HDI6500423.1"
                     /translation="MTNKDESVEKNTESTVEETNVKQNIDDSVEQAEESKGHLQDEAI
                     EETSDENVIEEIDPKDQKINELQQLADENEEKYLRLYAEFENYKRRIQKENEINKTYQ
                     AQRVLTDILPAIDNIERALQIEGDDETFKSLQKGVQMVHESLINALKDNGLEVIKTEG
                     EAFDPNIHQAVVQDDNPDFESGEITQELQKGYKLKDRVLRPSMVKVNQ"
     gene            6029..7861
                     /gene="dnaK"
                     /locus_tag="POB55_001458"
     CDS             6029..7861
                     /gene="dnaK"
                     /locus_tag="POB55_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HDI6500424.1"
                     /translation="MSKIIGIDLGTTNSCVTVLEGDEPKVIQNPEGSRTTPSVVAFKN
                     GETQVGEVAKRQAITNPNTVQSIKRHMGTDYKVDIEGKSYTPQEISAMILQNLKNTAE
                     SYLGEKVDKAVITVPAYFNDAERQATKDAGKIAGLEVERIINEPTAAALAYGLDKTDK
                     DEKVLVFDLGGGTFDVSILELGDGVFEVLSTAGDNKLGGDDFDQVIIDYLVAEFKKEN
                     GVDLSQDKMALQRLKDAAEKAKKDLSGVSQTQISLPFISAGENGPLHLEVNLTRSKFE
                     ELSDSLIRRTMEPTRQAMKDAGLTNSDIDEVILVGGSTRIPAVQEAVKKEIGKEPNKG
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIEILGGRMNTLIERNTTIPTSK
                     SQIYSTAVDNQPSVDVHVLQGERPMAADNKTLGRFQLTDIPPAERGKPQIEVTFDIDK
                     NGIVNVTAKDLGTNKEQRITIQSSSSLSDEEIDRMVKDAEVNAEADKKRREEVDLRNE
                     ADSLVFQVEKTLTDLGENIGEEDKKSAEEKKDALKTALEGQDIEDIKSKKEELEKVIQ
                     ELSAKVYEQAAQQQQQAQGANAGQNNDSTVEDAEFKEVKDDDKK"
     gene            7997..9136
                     /gene="dnaJ"
                     /locus_tag="POB55_001459"
     CDS             7997..9136
                     /gene="dnaJ"
                     /locus_tag="POB55_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HDI6500425.1"
                     /translation="MAKRDYYEVLGISKDASKDEIKKAYRKLSKKYHPDINKEEGADE
                     KFKEISEAYEVLSDDNKRASYDQFGHDGPQGFGGQGFNGSDFGGFSGFGGGGFEDIFS
                     SFFGGGRQRDPNAPQKGDDLQYTMTLTFEEAVFGTTKEISIRKDVTCETCHGDGAKPG
                     TSKKTCSYCNGAGHVAVEQNTILGRVRTEQVCPKCNGSGQEFEEACPTCHGKGTENKT
                     VKLEVKVPEGVDNEQQIRLAGEGSPGVNGGPAGDLYVVFRVKPSETFKRDGDDIYYKL
                     NVSFPQAALGDEIKIPTLNNEVMLTIPAGTQTGKQFRLKEKGIKNVHGYGYGDLYVDI
                     KVVTPTKLTDRQKELMKEFAQLNGEEINEQPSNFKDRAKRFFKGE"
     gene            9140..10078
                     /gene="prmA"
                     /locus_tag="POB55_001460"
     CDS             9140..10078
                     /gene="prmA"
                     /locus_tag="POB55_001460"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HDI6500426.1"
                     /translation="MNWTELSIIINHEAVELATNILENHGSNGVVIEDSDDLINQPED
                     KYGEIYALKKEDYPDKGVRLKAYFNEMTYDDKLRQQIKDELLNLDELDQHNVQFSEQI
                     IAETDWENEWKNYFHPFRASKKFTIVPSWETYAKEADEELCIELDPGMAFGTGDHPTT
                     SMCLKAIETYVLPQHSVIDVGTGSGILSIASHLIGVKRIKALDIDEMAVSVAKENFRR
                     NHCETLIEAVPGNLLKDETEKFDIVIANILAHIIDEMIEDAYNTLNEGGYFITSGIIK
                     EKYEGIQSHMERVGFKIISEQHDNGWVCLVGQKVSE"
     gene            10080..10832
                     /locus_tag="POB55_001461"
     CDS             10080..10832
                     /locus_tag="POB55_001461"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDI6500427.1"
                     /translation="MQRYFIDQNADVSQRFFITKKEDIHHITNVMRYDVGSKIILTFN
                     DQTVYLCEIADITSDKIAVDLKEKQNINTELPVDVTICSGLIKADKYEWLLQKATELG
                     ASSFIAVSMERSIVKLNEAKVAKKIERWQKIIKEAAEQSYRLVIPSIQFESNLKLICD
                     TIDNYDYILIAYEEEAKDGELSNFKQTLQQFKAQDKVLMIFGPEGGLSENEISLFSNT
                     STVVGLGPRILRAETAPLYALSAISYEKELMG"
     gene            10839..12185
                     /gene="mtaB"
                     /locus_tag="POB55_001462"
     CDS             10839..12185
                     /gene="mtaB"
                     /locus_tag="POB55_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HDI6500428.1"
                     /translation="MSTVAFHTLGCKVNHYETEAIWQLFKEANYERVDFEANADVFVI
                     NTCTVTNTGDKKSRQIIRRAIRQNPDAVICVTGCYAQTSSAEIMEIPGVDVVVGTQDR
                     HKLLGYIDEFRKERQPINGVGNIMKNRKYEELDVPYFTDRTRASLKIQEGCNNFCTFC
                     IIPWARGLMRSRDPEKVVEQATQLVNSGYKEIVLTGIHTGGYGQDLKDYNLAQLLRDL
                     ETINGLERIRISSIEASQLTDEVIDVLERSTKVVRHLHIPLQSGSDTVLKRMRRKYTM
                     DRFSERLTKLHKALPDLAVTSDVIVGFPGETEAEFQETYDFIVKHKFSELHVFPYSPR
                     IGTPAARMDDQIDEEIKNERVHKLITLSNQLGKLYASKFDQDVLEVIPEEQGDTEGTL
                     VGYADNYMKVQFEGDESLIGQIVKVKITQANYPLNEGQAIKVVDFATNKSDREVLV"
     gene            12478..12654
                     /locus_tag="POB55_001463"
     CDS             12478..12654
                     /locus_tag="POB55_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016998564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HDI6500429.1"
                     /translation="MSKTVVRKNESLEDALRRFKRSVSKSGTIQEVRKREFYEKPSVK
                     RKKKSEAARKRKFK"
BASE COUNT         4776 a         1752 c         2449 g         3772 t
ORIGIN      
        1 tatctttata aaaaataaaa gttcaagcat gcgcttgaac ttttatttaa aaagattatt
       61 tatttgcagt cattaattgt gatttaatac ggtcagcttt gtttgaatgt attaaattac
      121 tttgagcagc tttgtctact aacttaactg ctaagcttac taattcattt ttattatcag
      181 cgttatttga aacagctgtt ttagcgtttt taactgctgt acgcattgca ctcttttgtg
      241 aaatgttgcg tgcttcagct ttttcagttg tttttacacg tttaattgca gatttgatat
      301 ttgccatttc tgtcacctcc taaaagtgag ttcatcttat caaaatttta tttgattaca
      361 acaagaaata ttttaacaaa agtctaaccg ttgtgcaatc atttatttga tggggttagc
      421 ttaaaatttt gcagatttaa taaattgtgt tggaaacaca atcaatacgt tttataatta
      481 taagtacgta attaacatat taacatatcg caagtatgta tttaaataaa gattgttata
      541 atttcaaagt tcatccaaga ttatggcgtt tgcatttacc tattaaaaac gttattatat
      601 caaagatgcg aaagataata cgggtttatt ttatgaaagt gagaaggata aaatggataa
      661 tgagcaacgc ttaaaaagaa gagagaatat aaggaatttc tcgattatag cacatattga
      721 ccacggaaaa tctacattgg ctgatagaat tttagaaaat accaaatcag ttgaaacaag
      781 agatatgcaa gatcagttac tagattcaat ggatttagaa agagaacgtg gtattacaat
      841 caaattaaac gcagttcgtt taaagtacga agctaaagat ggaaatactt atacattcca
      901 tttaatcgat acgcctggac acgtcgattt tacatatgaa gtgtcacgtt ctttggcagc
      961 ttgtgagggc gcgattttag tagtagatgc ggctcaaggt atcgaagcac aaacattagc
     1021 aaatgtttat ttagcattag ataatgagtt agagttattg cctgttatta acaaaattga
     1081 tttacctgct gcagaacctg aacgcgtgaa acaagaaatt gaagatatga taggtttaga
     1141 ccaagacgat gttgttttag caagtgctaa atctaacatt ggaattgaag agatactaga
     1201 gaaaatagtt gaagttgtgc cagctccaga tggcgaccca gaagcaccac taaaagcgtt
     1261 aatatttgat tctgagtatg atccatatag aggggtaatt tcatcgataa gaattgtaga
     1321 cggtgttgtt aaagccggag ataaaattcg aatgatggcg actggtaaag agttcgaagt
     1381 aacagaagtt ggaattaata cacctaagca gcttccagtt gatgaattaa cagttggtga
     1441 tgttggttat attattgcaa gtattaaaaa tgttgatgat tctagggttg gtgacaccat
     1501 cacattagct agtagacctg catcagaacc attgcaaggt tataagaaaa tgaatccaat
     1561 ggtatattgc ggactgttcc caatagataa caaaaattat aatgatttaa gagaagcatt
     1621 agaaaaatta caattgaatg atgcatcatt agaatttgag cctgaatcgt cacaagcatt
     1681 aggttttggt tatagaactg gtttcttagg tatgttacac atggaaataa ttcaagaaag
     1741 aattgaaaga gaatttggca ttgaattaat tgcaacagca ccatctgtaa tttatcaatg
     1801 tattttaagg gacggttcag aagtgacggt tgataaccca gcacaaatgc cagatcgtga
     1861 taaaattgat aaaatatttg agccatatgt tcgtgcaact atgatggttc caaatgacta
     1921 tgtaggtgca gtaatggaat tatgtcaacg taaacgtgga caatttataa atatggacta
     1981 tttagatgat attcgtgtaa atattgttta tgaattacct ttagctgaag ttgtatttga
     2041 tttcttcgat caacttaaat ctaatactaa aggatatgca tcatttgatt atgaattcat
     2101 cgaaaataaa gaaagtaatt tagttaagat ggatatttta ttaaacggtg ataaagtgga
     2161 tgcgctaagc ttcatagttc atagagattt tgcatatgaa cgtggtaaag cattagttga
     2221 aaaacttaaa acgttaattc caagacagca atttgaagta cctgtacagg ctgcaatagg
     2281 acaaaaaatt gtagcgcgta caaatattaa atcaatgggt aaaaacgttt tagctaaatg
     2341 ttatggcggt gacataagcc gtaaacgtaa attacttgaa aaacaaaaag caggtaaagc
     2401 taagatgaaa gcagttggta atgttgaaat tccacaggat gctttcttgg ctgtattgaa
     2461 aatggatgac gaataagttt tttaaaaatt tattaacaat ttacaatgaa taaagtttaa
     2521 taactaaaaa gagggagcct aggataaatt aacgtcctgg gctttacaat gttatattgg
     2581 cagccatcga cagagttaaa aatgagctta taacaatggg gccccaacat agaagctgac
     2641 gaaaagtcag cttacaataa tgtgcaagtt ggggtggggc cccaacatag agaatttcga
     2701 aaagaaattc tacaggcaat gcaagttggg gtggggcccc aacatagaga aattggattg
     2761 ccaatttcta cagacaatgc aagttggggt gggacaacga aataaatttt gcgaaaatat
     2821 catttctgtc ccactccctt atgcatgagt tttactcatg taattttatt tttatggaca
     2881 tattacatct ggctaatgtg taagagccac tacataataa atcattagtg gttctttatt
     2941 atttctatct cactccctct aaacaagaat aaatatcaaa atgaatcgat atattagaca
     3001 accattgatt aaacgttaaa gttaaaagta agaataattg cagatagtcc aacaggatat
     3061 agccgattgg ataaacagtc tgagaagcgg ggcattaaaa tgacggtaca aagtgcatat
     3121 atacatattc cattttgtgt aagaatatgt acatattgtg atttcaataa atattttata
     3181 cagaatcaac ctgtagatga gtacttagat gcactaataa cagaaatgtc tacagcaaaa
     3241 tataggaact taaagaccat gtatgtaggt ggcggcacac caacggccct ttctattaat
     3301 cagttggaaa gattacttaa agcaatacgt gatacgttta caatcacagg cgagtataca
     3361 tttgaagcaa atcctgatga gttaactaaa gagaaagtcc aactattaga aaaatatgga
     3421 gtaaacagaa tttcaatggg cgttcaaaca ttcaagccgg agttattgtc tgttttaggt
     3481 agaacgcaca atactgaaga tatttacact tcggtgttaa atgctaaaaa cgcaggtatt
     3541 aaatcaatca gtttagattt aatgtatcat ttaccgaaac agacgattga agattttgaa
     3601 caaagtttag atctagcttt agatatggat attcaacata tttcgagtta cggcttaata
     3661 cttgaaccta aaacccaatt ttataatatg tatagaaaag ggttgcttaa acttcctaat
     3721 gaggatttag gtgctgacat gtatcagttg ctgatgtcta agatagaaca atctcctttc
     3781 catcaatacg aaatatctaa ttttgcatta gatggccatg aatcagaaca taataaggtt
     3841 tactggttta atgaggaata ttatggattt ggagcaggtg caagtggtta tgtagatggt
     3901 gtgcgttata cgaatatcaa tccagtgaat cattatatca aagctataaa taaagaaagt
     3961 aaagcaattt tagtatcaaa taaaccttct ttgactgaga gaatggaaga agaaatgttt
     4021 cttggtttgc gtttaaatga aggtgtgagt agtagtaggt tcaaaaagaa gtttgaccaa
     4081 tctattgaaa gtgtctttgg tcaaacaata aataatttaa aagagaagga attaattgta
     4141 gaaaagaacg atgcgattgc actaacaaag cgagggaaag tcataggtaa tgaggttttt
     4201 gaagctttcc taataaatga ttaaaaaaaa ttgaaatttc gagtctttaa cattgactta
     4261 ctttgaccaa tttgataaat tataattagc acttgagata agtgagtgct aatgaggtga
     4321 aaacatgatt acagataggc aattgagtat attaaacgca attgttgagg attatgttga
     4381 ttttggacaa cccgttggtt ctaaaacact aattgagcga cataacttga atgttagtcc
     4441 tgctacaatt agaaatgaga tgaaacagct tgaagattta aactatatcg aaaagacaca
     4501 tagttcttca gggcgttcgc catcacaatt aggttttagg tattatgtca atcgtttact
     4561 tgaacaaaca tctcatcaaa aaacaaataa attaagacga ttaaatcaat tgttagttga
     4621 gaatcaatat gatgtatcat cagcattgac atattttgca gatgaattat caaatatatc
     4681 tcaatataca actttagttg ttcatcctaa tcataaacaa gatattatca ataatgtaca
     4741 cttgattcgt gctaatccta atttagttat aatggttatc gtattttcat caggtcatgt
     4801 tgagcatgta catcttgctt cagacatacc gtttaataat gacaaactaa atacaatatc
     4861 aaattttgtt acaaacaaac taactgagtt taatcagaat ttacaagatg atattgtctc
     4921 ttttgttcaa tcagagcagg aagaaatatt tatcaataaa ttgttaaata cgatgaataa
     4981 tcatatttct aatcaaagta atagcattta tatgggtgga aaggttaaac ttattgatgc
     5041 attgaatgaa agtaatgtat cttcaattca gcccattcta caatatatag aatcaaatag
     5101 aattgcagaa ttattacaag atatatcttc accaaatatt aatgtaaaaa ttggtaatga
     5161 aattgatgac agcttaagtg atatttcaat tgtaacaagt caatatcatt ttgatgaaac
     5221 gttaaaaggt caaatcgctg tgattggacc tacagctatg cattatcaaa atgtcattca
     5281 attattaaat agaatttggt aatgatggat tagaaagtat tggaggacag aaaatgacaa
     5341 ataaagacga atcagttgaa aaaaacactg aatcaacagt tgaagaaaca aacgtcaaac
     5401 aaaatattga tgattcagtt gaacaagctg aagaaagtaa aggtcattta caagatgaag
     5461 caatagaaga aacgtctgac gagaatgtta ttgaagaaat agatccaaaa gatcaaaaaa
     5521 ttaatgaact tcaacaatta gcagatgaaa acgaagagaa atatttaagg ctctacgctg
     5581 agtttgaaaa ttataagcgt agaattcaaa aagaaaatga aataaataaa acatatcaag
     5641 cacaacgtgt gttaacagat attttaccag caatagacaa tatagaacgt gcacttcaaa
     5701 ttgaaggtga tgatgagact tttaaatctc ttcaaaaagg tgtacaaatg gtgcatgaaa
     5761 gtttgattaa cgcactaaaa gataatggtc ttgaagttat taaaactgaa ggtgaagcat
     5821 ttgatccaaa tattcaccaa gctgtagttc aagatgataa ccctgatttt gaatctggcg
     5881 aaatcactca agaactacaa aaaggataca agcttaaaga tagagtatta agaccatcaa
     5941 tggtcaaagt aaaccaataa cttaaatttg gcgaaaagac attgtttaaa attaaattaa
     6001 tttaatgatt aaatggagga attttattat gagtaaaatt attggtatag acttaggtac
     6061 aacaaattca tgtgtaacag tattagaagg cgatgagcca aaagtaattc aaaaccctga
     6121 aggttcacgt acaacaccat ctgttgtagc tttcaaaaat ggagaaactc aagttggtga
     6181 agtagctaaa cgtcaagcta ttacaaaccc aaacactgtt caatctatta aacgtcatat
     6241 gggtactgat tataaagtag atattgaagg taaatcatac acaccacaag aaatctcagc
     6301 tatgatttta caaaacttaa aaaatacagc tgaaagctat ttaggtgaga aagttgacaa
     6361 agctgtaatt acagtacctg catactttaa cgatgctgaa cgtcaagcaa ctaaagatgc
     6421 tggtaaaatt gctggtttag aagttgagcg tatcattaat gaaccaacag ctgcagcatt
     6481 agcatatggt ttagataaaa ctgataaaga tgaaaaagtt cttgtttttg acttaggtgg
     6541 cggtacattt gacgtatcta tcctagaatt aggtgacggt gtattcgaag tactatcaac
     6601 agccggtgac aacaaacttg gcggtgatga ttttgaccaa gtaattattg actacctagt
     6661 tgcagaattc aaaaaagaaa atggcgtaga cttatctcaa gataaaatgg cattacaacg
     6721 tttgaaagat gctgctgaaa aagctaaaaa agacttatca ggtgtatcac aaactcaaat
     6781 ctcattacca tttatctcag ctggtgaaaa cggtccatta cacttagaag taaacttaac
     6841 tcgttctaaa tttgaagaat tatcagattc attaattaga agaacaatgg aacctacacg
     6901 ccaagcaatg aaagacgctg gcttaacaaa ctcagatatc gatgaagtta tcttagttgg
     6961 tggatcaact cgtattccag cagtacaaga agctgtcaaa aaagaaatcg gtaaagaacc
     7021 taacaaagga gtaaacccgg acgaagtagt ggcaatggga gctgcaatcc aaggtggcgt
     7081 tatcacaggt gacgttaaag acgtagtatt attagacgta acaccactat ctttaggtat
     7141 tgaaatttta ggtggacgta tgaatacgtt aattgaacgt aacactacga ttcctacatc
     7201 taaatctcaa atctattcaa cagcagtaga taatcaacca tcagtagatg tacacgtatt
     7261 acaaggtgaa cgtccaatgg ctgcggataa taaaacactt ggtagattcc aattgactga
     7321 tattccacca gctgaacgtg gtaaacctca aattgaagta acgtttgata tcgataaaaa
     7381 cggtattgta aatgtaactg caaaagactt aggtacaaat aaagaacaaa gaattacaat
     7441 tcaatcaagt tcttcattat cagacgaaga aatcgaccgt atggtaaaag atgctgaagt
     7501 taacgctgaa gcagataaaa aacgtcgtga agaagtagac ttaagaaacg aagctgacag
     7561 tctagtattc caagttgaaa aaactttaac tgatttaggc gaaaatatcg gtgaagaaga
     7621 taaaaaatct gctgaagaga aaaaagacgc tcttaaaact gctttagaag gtcaagatat
     7681 agaagatatt aaatctaaaa aagaagaact tgaaaaagtg attcaagaat tatcagcaaa
     7741 agtatatgag caagcggctc aacagcaaca acaagcacaa ggtgcaaatg ctggtcaaaa
     7801 caacgatagt actgtagaag atgctgaatt taaagaagta aaagacgacg acaaaaaata
     7861 attaaacaat acaattagaa tgttgtcatg atctgaaaag tcaaagtcaa tagaacattg
     7921 gctttggctt tttatttgga tagtatgaca tattacgtta aactatatga gttaattaag
     7981 aaaaggagag atagctgtgg ccaaaagaga ctattatgaa gttttaggca ttagtaaaga
     8041 tgcttcaaaa gatgaaatca aaaaagcgta tcgaaagctt tcgaaaaaat atcatccaga
     8101 tattaacaaa gaagaaggtg cagatgaaaa gtttaaagaa atttctgaag cctatgaagt
     8161 tttaagtgat gataataaac gcgcaagcta tgatcaattt ggacatgatg gtccacaagg
     8221 ttttggtgga caaggattca atggctctga ctttggcggt tttagtggct tcggtggcgg
     8281 cggttttgaa gatatcttta gctctttctt cggtggcggc agacaaagag atccaaatgc
     8341 accgcaaaaa ggtgatgatc ttcagtatac aatgacatta acatttgaag aagcggtatt
     8401 tggtacaaca aaagaaattt caatccgtaa agatgtaaca tgcgaaacat gtcatggtga
     8461 tggtgcaaag cctggaacaa gtaaaaagac ttgtagttac tgtaatggcg ctggtcatgt
     8521 agctgtagaa caaaacacaa ttttaggtag agttcgtact gaacaagttt gtcctaaatg
     8581 taatggaagt ggtcaagaat ttgaagaagc ttgtccaaca tgtcacggaa aaggtactga
     8641 aaataaaaca gttaaattag aagtgaaagt acctgaaggt gtcgacaatg aacaacaaat
     8701 tagattagct ggtgaaggct ctccaggcgt gaatggagga cctgctggtg atttatacgt
     8761 agtatttaga gttaaaccat ctgaaacatt taaacgtgat ggtgatgata tttattacaa
     8821 attaaatgtt agcttcccac aagcagcttt aggcgatgaa ataaaaatac caacattaaa
     8881 taatgaagtt atgttaacaa tccctgctgg tacacaaaca ggtaagcaat tccgcttaaa
     8941 agaaaagggt ataaaaaatg ttcatggcta tggatacgga gatttatatg ttgatattaa
     9001 agtagtaacg cctactaaat taacagatag acaaaaagaa ctaatgaaag aatttgccca
     9061 attaaatggt gaagaaatta atgaacaacc ttcgaatttt aaagatagag caaaaagatt
     9121 ctttaaggga gaataaagta tgaactggac agagctttca attattatta atcatgaagc
     9181 agtagaattg gctaccaata tacttgaaaa tcatggatca aatggtgtcg tgatagaaga
     9241 ttcagatgat ttaattaacc aaccagaaga taaatacggt gaaatttacg ctttgaaaaa
     9301 agaggattat ccagataagg gagtaagatt gaaagcctat tttaatgaaa tgacttatga
     9361 tgataagttg cgacagcaaa ttaaagatga gttattaaat ttagatgaac ttgatcaaca
     9421 taacgttcaa ttcagtgagc aaattattgc agagacggat tgggaaaatg aatggaaaaa
     9481 ctatttccat ccattccgag cgtcgaagaa gttcacaata gttcctagtt gggaaacata
     9541 tgctaaagaa gcggatgaag agctttgcat tgagctcgac ccaggtatgg cttttggaac
     9601 aggtgatcat ccgactacaa gtatgtgttt gaaggcaata gaaacatatg tattgccaca
     9661 gcattcagta attgatgttg gtactggctc aggtatatta agtattgcaa gtcatctaat
     9721 cggtgtaaaa cgtattaaag cgttggatat tgatgaaatg gcagtgagtg tagctaaaga
     9781 aaacttcaga agaaatcatt gtgaaacgtt aattgaagct gttccaggta acttattgaa
     9841 agacgaaaca gaaaaatttg atattgtaat agcaaatatt ttagcgcata ttattgatga
     9901 aatgattgaa gatgcttata atactctaaa tgaaggcggt tattttatta cttctggtat
     9961 tataaaagag aagtatgaag gtatacagtc acatatggag cgtgtaggtt ttaaaattat
    10021 ttcagaacaa catgacaacg gttgggtttg tcttgttggc cagaaagtga gtgaataatg
    10081 tgcaacgtta tttcatagac caaaacgctg atgtaagtca gcgttttttt attacaaaaa
    10141 aagaagatat tcatcatata acaaatgtaa tgagatatga tgttggtagt aaaattattt
    10201 taacttttaa tgatcaaaca gtttatttat gtgaaattgc tgatataaca agtgataaaa
    10261 ttgcagttga tttaaaagaa aaacaaaata ttaatacaga attgccagtt gatgttacga
    10321 tttgcagtgg actaatcaaa gctgacaaat atgagtggtt gctacaaaaa gctactgaat
    10381 tgggtgcttc atcatttata gctgtgagca tggaacgttc aattgttaaa ttaaacgagg
    10441 caaaagttgc caaaaagata gagcgttggc aaaaaataat taaagaagct gctgaacaaa
    10501 gctatcgttt agtgattcca tctattcaat tcgagtcgaa tttaaaatta atttgtgata
    10561 ctattgataa ttatgactat attcttattg catatgaaga ggaagcaaaa gatggtgagt
    10621 taagcaattt caagcaaact ttacaacaat tcaaggctca ggataaagtg ttgatgattt
    10681 ttggtcctga gggtggtttg tcagaaaatg aaatttcatt atttagtaat actagtacag
    10741 ttgttggtct tggaccgaga attttacggg cagaaactgc gccattgtat gcgttaagtg
    10801 caataagtta tgaaaaagaa ttaatggggt gaatatttat gtcaacagtt gcgtttcaca
    10861 cattaggttg taaagtaaac cattatgaaa ctgaagcaat ctggcaatta tttaaagaag
    10921 caaactatga gcgcgttgac tttgaagcga atgctgatgt atttgttatt aatacttgta
    10981 cagtaacgaa tacaggtgat aaaaaaagtc gtcaaataat tagacgtgca ataagacaaa
    11041 atcctgatgc tgtaatctgt gtaacaggtt gttatgcgca aacttcatca gctgaaatta
    11101 tggaaattcc tggtgtcgat gtagtagttg gtacacaaga tagacataaa ctattaggtt
    11161 acattgatga attccgtaaa gaacgccaac caattaatgg tgttggaaat atcatgaaaa
    11221 atcgtaaata tgaagaatta gatgtcccat attttacaga tagaacacgt gcgtcattaa
    11281 aaattcaaga aggttgtaac aacttctgca cattctgtat tattccatgg gctcgtggct
    11341 taatgcgttc aagagatccg gaaaaagtag ttgaacaagc gacgcaacta gtgaattcag
    11401 gatataagga aattgtattg acgggaattc atacaggtgg atatggtcaa gatttaaaag
    11461 attataactt ggcccaatta ttacgtgatc ttgaaacgat taatggatta gaacgaattc
    11521 gaatttcttc aattgaagca agtcaactta cagatgaagt aattgacgtt ttagaacgtt
    11581 caacaaaagt tgtgcgtcat ttgcatattc cattacaatc tggttcagat acagtattaa
    11641 aacgtatgag acgtaagtat acaatggata gattttcaga acgattaaca aaattgcata
    11701 aagctttacc agacttggca gttacgagtg atgtaattgt tggtttccca ggtgaaactg
    11761 aagcagagtt ccaagaaaca tatgatttta tcgtaaagca taagttctct gaactgcatg
    11821 ttttccctta ttctcctaga attggcacgc cagctgcaag aatggatgac caaattgatg
    11881 aagaaattaa aaatgaacgg gtgcataagt taattacgct aagcaatcaa ctcggaaagt
    11941 tatatgcgtc taaatttgat caagatgtgc ttgaagtaat tcctgaggaa cagggtgaca
    12001 cagaaggcac attagttgga tatgcagata attatatgaa agtacaattt gaaggtgacg
    12061 aatcactcat aggtcaaatt gtaaaagtta aaattacgca agcaaattat ccattaaatg
    12121 aagggcaagc aattaaagtt gttgatttcg caacaaataa atctgataga gaagttttag
    12181 tttaagtatt ccttgattca taccataagg ttccgtatta aagatatagg ttttgtaacg
    12241 ttgttttaac ggcttcacaa tttactatat aagcaactct tctcattaaa taaattatta
    12301 agagattttt agaacaaaat ctaaaagtct ttgtgatttt gatgtgaaat atgtttcaaa
    12361 taaaaaacaa tttactaatt gaccataaat tacagatata ttatacttat aaatgcatag
    12421 ttttactgtg caattgacta taaagttccg ttgatatttg gagggaggga aatacagatg
    12481 tctaaaacag tagtacgtaa aaatgaatca cttgaagatg cgttacgtag atttaaacgt
    12541 tcagtttcta aaagtggaac aatccaagaa gtacgtaaac gtgaatttta cgaaaaacca
    12601 agcgtaaaac gtaaaaagaa atcagaagct gcacgtaaac gtaaattcaa ataattaata
    12661 cctctgttga ctccctcaac acgaatatta attatataaa acaaacatca caagttagtg
    12721 tcttacacta atatgtgatg tttttttgt
//
LOCUS       DAMZKC010000071        12657 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.071, whole genome shotgun sequence.
ACCESSION   DAMZKC010000071 DAMZKC010000000
VERSION     DAMZKC010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12657
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.071"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            132..2228
                     /gene="pnp"
                     /locus_tag="POB55_001464"
     CDS             132..2228
                     /gene="pnp"
                     /locus_tag="POB55_001464"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HDI6500430.1"
                     /translation="MSQEKKVFKTEWAGRSLTIETGQLAKQANGAVLVRYGDTVVLST
                     ATASKEPRDGDFFPLTVNYEEKMYAAGKIPGGFKKREGRPGDDATLTARLIDRPIRPL
                     FPKGYKHDVQIMNMVLSADPDCSPQMAAMIGSSMALSVSDIPFQGPIAGVNVGYIDGK
                     YIINPTVEEKEVSRLDLEVAGHKDAVNMVEAGASEITEQEMLEAIFFGHEEIQRLVDF
                     QQQIVDHIQPVKQEFIPVERDEALVERVKSLTEEKGLKETVLTFDKQQRDENLDNLKE
                     EIVNEFIDEEDPENELLIKEVYAILNELVKEEVRRLIADEKIRPDGRKPDEIRPLDSE
                     VGILPRTHGSGLFTRGQTQALSVLTLGALGDYQLIDGLGPEEEKRFMHHYNFPNFSVG
                     ETGPVRAPGRREIGHGALGERALKYIIPDTADFPYTIRIVSEVLESNGSSSQASICGS
                     TLALMDAGVPIKAPVAGIAMGLVTREDSYTILTDIQGMEDALGDMDFKVAGTKEGITA
                     IQMDIKIDGLTREIIEEALEQARRGRLEIMNHMLQTIDQPRTELSAYAPKVVTMTIKP
                     DKIRDVIGPGGKKINEIIDETGVKLDIEQDGTIFIGAVDQAMINRAREIIEEITREAE
                     VGQTYQATVKRIEKYGAFVGLFPGKDALLHISQISKNRIEKVEDVLKIGDTIEVKITE
                     IDKQGRVNASHRALEE"
     gene            2464..4137
                     /locus_tag="POB55_001465"
     CDS             2464..4137
                     /locus_tag="POB55_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002455407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDI6500431.1"
                     /translation="MSLIKKKNKDIRIIPLGGVGEIAKNMYIVEVDDEMFMLDAGLMF
                     PEDEMLGIDIVIPDISYVLENKDKLKGIFLTHGHEHAIGAVSYVLEQLDAPVYGSKLT
                     IALIKENMKARNIDKKVRYYTVNNDSIMRFKNVNISFFNTTHSIPDSLGVCIHTSYGA
                     IVYTGEFKFDQSLHGHYAPDIKRMAEIGEEGVFVLISDSTEAEKPGYNTPENVIEHHM
                     YDAFAKVRGRLIVSCYASNFIRIQQVLNIASKLNRKVSFLGRSLESSFNIARKMGYFD
                     IPKDLLIPITEVDNYPKNEVIIIATGMQGEPVEALSQMAQHKHKIMNIEEGDSVFLAI
                     TASANMEVIIANTLNELVRAGAHIIPNNKKIHASSHGCMEELKMMINIMKPEYFIPVQ
                     GEFKMQIAHAKLAAEAGVAPEKIFLVEKGDVINYNGKDMILNEKVNSGNILIDGIGIG
                     DVGNIVLRDRHLLAEDGIFIAVVTLDPKNRRIAAGPEIQSRGFVYVRESEDLLREAEE
                     KVREIVEAGLQEKRIEWSEIKQNMRDQISKLLFESTKRRPMIIPVISEI"
     gene            4394..6763
                     /locus_tag="POB55_001466"
     CDS             4394..6763
                     /locus_tag="POB55_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDI6500432.1"
                     /translation="MAQAKKKSTAKKKTTSKKRTNSRKKKNDNPIRYVIAILVVVLMV
                     LGVFQLGIIGRLIDSFFNYLFGYSRYLTYILVLLATGFITYSKRIPKTRRTAGSIVLQ
                     IALLFVSQLVFHFNSGIKAEREPVLSYVYQSYQHSHFPNFGGGVLGFYLLELSVPLIS
                     LFGVCIITILLLCSSVILLTNHQHREVAKVVLENIKAWFGSFNEKMSERNQEKQLKRE
                     EKARLKEEQKARQNEQPQIKDVSDFTEVPQERDIPIYGHTENESKSQSQPSRKKRVFD
                     AENSSNNIVNHHQADQQEQLTEQTHNSVESENTIEEAGEVTNVSYVVPPLTLLNQPAK
                     QKATSKAEVQRKGQVLENTLKDFGVNAKVTQIKIGPAVTQYEIQPAQGVKVSKIVNLH
                     NDIALALAAKDVRIEAPIPGRSAVGIEVPNEKISLVSLKEVLDEKFPSNNKLEVGLGR
                     DISGDPITVPLNEMPHLLVAGSTGSGKSVCINGIITSILLNAKPHEVKLMLIDPKMVE
                     LNVYNGIPHLLIPVVTNPHKAAQALEKIVAEMERRYDLFQHSSTRNIKGYNELIRKQN
                     QELDEKQPELPYIVVIVDELADLMMVAGKEVENAIQRITQMARAAGIHLIVATQRPSV
                     DVITGIIKNNIPSRIAFAVSSQTDSRTIIGTGGAEKLLGKGDMLYVGNGDSSQTRIQG
                     AFLSDQEVQDVVNYVVEQQQANYVKEMEPDAPVDKSEMKSEDALYDEAYLFVVEQQKA
                     STSLLQRQFRIGYNRASRLMDDLERNQVIGPQKGSKPRQVLIDLNNDEV"
     gene            6768..7481
                     /locus_tag="POB55_001467"
     CDS             6768..7481
                     /locus_tag="POB55_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDI6500433.1"
                     /translation="MSEMNAVYNVKQYILNLIKQNKLEYGDQLPSNLSIARELNVKTD
                     DVYEAIQALITEQVIKDNFEEGTSVKSLPPFFYPLNELISIGQMIKNAGFECGTEYLN
                     FDEQPATMLDANLLSVEEGYPVTIIERLRTADGEPVVYCLDKIAKKELTCTEYQMSNG
                     SILSAIKEQSNHNICYADTEIEAVNYEPRISEVLNASPHEGLILLKITHYNELDEPIL
                     YSLNYMKNSLVQFKITRKI"
     gene            7512..8777
                     /locus_tag="POB55_001468"
     CDS             7512..8777
                     /locus_tag="POB55_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDI6500434.1"
                     /translation="MSRQSQPNIHIKVSPTTKFKTTTIVFKFMAPLEYDTTTARSLLS
                     KLLVRATKKWPTDKSFNNHLADLYGAYVNSTISKFKDQHVITFSLEIVNERYLRNGES
                     LFNQGLDLLQEIIWNPLIENKAFNDNFVNQEKTLLAKKIEAMVDNKAQYSFLKLLDHM
                     FENEAYKYLSTGQLEQIPHITAETLYHTYQSMINNDQCSVYVVGNVEPESVEKQIREK
                     FALKPFDKHQFQHSTHHLHDEEVDYIVEYDDVDQAKLNMGYRFPTQYGQSGYAAFVVF
                     NMMFGGDPSSVLFNEVREKQSLAYSIHSQIDGKNGYLFVLSGVSSDKYETAKDTIISE
                     FEKIKAGDFTEEKLELAKKVIISHRYESEDRPKSIIEIMHNQILLEQPQSKETFINDI
                     QKVSREDIVSVAEKAFLDTIYVLTKGGDK"
     gene            8777..10063
                     /locus_tag="POB55_001469"
     CDS             8777..10063
                     /locus_tag="POB55_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDI6500435.1"
                     /translation="MKERYYELIDERVFEQELENGLRLFVIPKPGFQKTFVTYTTQFG
                     SLDNQFKPLGQDQFVTVPDGVAHFLEHKLFEKEEEDLFTAFAEDNAQANAFTSFDRTS
                     YLFSATDNIENNIKRLLTMVETPYFTKETVDKEKGIIAEEIKMYQEQPGYKLMFNTLR
                     AMYQQHPIRVDIAGSVESIYDITKDDLYLCYETFYHPSNMVLFVVGDVDPEAICRIVK
                     QHEDARNKVNQPKIERGLVNEPEDVKEAFVTESMKIQSPRLMLGFKNKPLQEAPQKYV
                     QRDLEMSLFFELIFGEETDFYQNLLNEGLIDDTFGYQFVLEPTYSFSIVTSATEEPDK
                     LKKLLLDELRDKKGNFQDAEAFELLKKQFIGEFISSLNSPEYIANQYTKLYFEGVSVF
                     DMLDIVENITLDSINETSSLYLNLDQQVDSRLEIKK"
     gene            10063..10767
                     /locus_tag="POB55_001470"
     CDS             10063..10767
                     /locus_tag="POB55_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HDI6500436.1"
                     /translation="MKALVLGGSGSIGSEIVKQLLTDGFEVYVQYYRTDINELTSKFN
                     DDKVRFIQADLSQTIDIDKTFGDIKSLDCLIYASGQSLYGVLQDMKDHDIDACYQLNV
                     LQLIRLCRYFVDVLRQSDNGRIIVISSIWGETGASMETIYSTMKSAQLGFVKALSQEL
                     ALTSVTVNAIAPGFVAGNMASEWQEDELQAMITELPQQRLILPSEVAHTCAYLYHPNA
                     RSVTGTIQKVNGAWYI"
     gene            10872..11699
                     /locus_tag="POB55_001471"
     CDS             10872..11699
                     /locus_tag="POB55_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfK family protein"
                     /protein_id="HDI6500437.1"
                     /translation="MTVAEKKEWYLEYEIEINRPGLLGDVSSLLGMLGISIVTINGVD
                     QGKRGLLIKTDNLEKVERFEQIARGINEIEITKLKKPELRDRLAVRHGRYIEQDAKDK
                     KTFRFEREDLGLLVDFLAELFKEEGHKLIGIRGMPRVGKTESIVAGSVCAHKRWLFIS
                     STLIKQTVRSSLIKGEYDANHVYIIDGAVTARESNPKHQELVNEVMTLPSIKVVEHPD
                     LFVETSTCTMEDFDYIIELRENENQEIHYEEMKKQTVQSKNNLDFGDPFGGGFGFFE"
     gene            11718..12110
                     /locus_tag="POB55_001472"
     CDS             11718..12110
                     /locus_tag="POB55_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI6500438.1"
                     /translation="MKTVGEALKGRRERLGMTLTELEQRTGIKREMLVHIENNEFDQL
                     PNKNYSEGFIRKYASVVNIEPNQLIQAHQDEIPSNQAEWDEVITVFNNNKDLDYKSKS
                     KEPIQLLVIMGITVLITLLLWIMLVLIF"
     gene            12144..>12657
                     /gene="pgsA"
                     /locus_tag="POB55_001473"
     CDS             12144..>12657
                     /gene="pgsA"
                     /locus_tag="POB55_001473"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HDI6500439.1"
                     /translation="MNIPNQITVFRVVLIPVFILFALVDFGFGNVSFLGGYEIRIELL
                     ISGFIFILASLSDFVDGYLARKWNLVTNMGKFLDPLADKLLVASALIVLVQLGLTNSV
                     VAIIIIAREFAVTGLRLLQIEQGFVSAAGQLGKIKTAVTMVAITWLLLGDPLATLIGL
                     SLGQILLYIGV"
BASE COUNT         4613 a         1705 c         2452 g         3887 t
ORIGIN      
        1 taagtctatt ttgtaaataa tagatggata tcccgctctc tttttttcca attcaatatt
       61 ttataactaa tattaaaata cgataataaa tgatatgata taactattag attcaagaga
      121 ggagatttat aatgtctcaa gaaaagaaag tttttaaaac tgaatgggca ggaagatctt
      181 taacgattga aacagggcaa ttagctaaac aagcaaatgg cgctgtattg gttcgttatg
      241 gagatacagt cgtgttatcg acggcaactg catcaaaaga acctcgtgat ggagatttct
      301 tcccattaac agtgaactat gaagaaaaaa tgtacgctgc gggtaaaatt cctggtggat
      361 ttaaaaagag agaaggacgt cctggtgacg atgcaacatt aactgcgcga ttaattgata
      421 gaccaattag acctttattc cctaaaggat ataagcatga tgttcaaatt atgaacatgg
      481 tattaagtgc agatcctgat tgttcaccac aaatggctgc aatgattggt tcatctatgg
      541 cgcttagtgt gtcggatatt ccattccaag ggccaatcgc cggtgtaaat gtgggttata
      601 ttgacggtaa atatatcatt aacccaacag tagaagaaaa agaagtttct cgcttagacc
      661 ttgaagtagc tggtcataaa gatgcagtaa acatggtaga ggcaggcgct agtgagatta
      721 ctgaacaaga aatgttagag gcgattttct ttggtcatga agagattcaa cgtttagttg
      781 atttccaaca acaaatcgtc gaccacattc aacctgttaa acaagaattt attccagtag
      841 agcgtgatga agcgctagtt gaacgtgtaa aatctttaac cgaagaaaag ggacttaaag
      901 aaacagtttt aacatttgat aaacaacaac gagatgaaaa tcttgataac ttaaaagaag
      961 aaatcgtcaa tgaatttatc gatgaagaag atccagaaaa tgaattactt attaaagaag
     1021 tttatgcaat tttaaatgaa ttagtgaaag aagaagttcg acgtttaatt gcagatgaaa
     1081 aaattagacc agacggccgt aaacctgatg aaatccgtcc attagattct gaagttggta
     1141 ttttacctag aacgcatggt tcaggtctat ttacacgtgg tcagactcaa gcactttcag
     1201 ttttaacatt aggtgcttta ggcgattatc aattaattga tggtttagga cctgaagaag
     1261 aaaaaagatt catgcatcat tacaacttcc cgaatttttc agtaggtgaa actggtccag
     1321 tacgtgcgcc aggtcgtcgt gaaattggac atggtgcgtt aggtgaaaga gcattaaaat
     1381 atattattcc tgatactgct gatttcccat atacaattcg tattgtaagt gaggtacttg
     1441 aatcaaatgg ttcatcatct caagcgtcaa tttgtggatc aacattagca ttaatggatg
     1501 cgggcgtacc gattaaagca ccagttgctg gtattgctat gggccttgtt acacgtgaag
     1561 atagctatac gattttaact gatatccaag gtatggaaga tgcattaggt gatatggact
     1621 ttaaagtcgc tggtactaaa gaaggtatta cagcaatcca aatggatatt aaaattgacg
     1681 gtttaacgcg tgaaattatc gaagaggctc tagaacaagc gagacgtggt cgtttagaaa
     1741 taatgaatca tatgttacaa acaattgatc aaccacgtac tgaattaagt gcttacgcgc
     1801 caaaagttgt aactatgaca attaaaccag ataagattag agatgttatc ggacctggtg
     1861 gtaaaaaaat taacgaaatt attgatgaaa caggcgttaa attagatatt gaacaagatg
     1921 gtactatctt tattggtgcc gttgatcaag ctatgataaa tcgcgctcgt gaaatcattg
     1981 aggaaattac acgtgaagcg gaagtaggtc aaacttatca agccactgtt aaacgtattg
     2041 aaaaatacgg tgcgtttgta ggcctattcc cgggtaaaga tgcgttgctt cacatttcac
     2101 aaatttcaaa aaatagaatt gaaaaagtgg aagatgtatt aaaaatcggt gacacaattg
     2161 aagttaagat tactgaaatt gataaacaag gtcgagtaaa tgcttcacac agagcattag
     2221 aagaataata tttaaagtca tatgacgaca atgtatcgtc atgtgatttt tttatgccat
     2281 tttttacgaa gtgatccaat ttgaatttgt tgtattgaac attttaaaac gctttattat
     2341 tttgtgtgca actgttaatt atcctgtatg tatagtgatt aatagtgtac atcaagtgtt
     2401 ttttaactta taatgaatag tgagtttata tatggacggg taacaaattt aggaggtaag
     2461 attttgagtt taataaagaa aaagaataaa gatattcgca ttataccatt aggcggtgtt
     2521 ggcgaaattg ctaaaaatat gtatatcgtt gaagtagacg atgaaatgtt tatgttagat
     2581 gctggactta tgtttccaga agacgaaatg ctaggtattg atattgttat accagacatt
     2641 tcatacgtac ttgaaaataa agataaattg aagggtatat tccttacaca cggacatgag
     2701 cacgcgattg gtgcagtgag ttatgtttta gaacaattag atgcaccagt atatggatct
     2761 aaattgacaa tagcgttaat taaagaaaat atgaaagccc gtaatattga taaaaaagtt
     2821 cgctactaca cagttaataa tgattcaatt atgagattca aaaacgtgaa tattagtttc
     2881 tttaatacga cacacagtat tcctgatagt ctaggtgttt gtattcacac ttcatatggt
     2941 gccattgtgt atacaggtga atttaagttt gaccaaagtt tacatggaca ttatgcacca
     3001 gatattaaac gtatggcaga gattggtgaa gaaggtgtat ttgtcttaat cagtgattct
     3061 actgaggcag agaaacctgg atataatact ccggaaaatg tgattgaaca tcatatgtat
     3121 gacgcttttg caaaagtgcg aggtcgcttg attgtttcat gttatgcttc gaactttata
     3181 cgtatacagc aagttttaaa tattgctagc aagctaaatc gtaaagtgtc atttttagga
     3241 agatcacttg aaagttcatt taatattgct cgtaaaatgg ggtatttcga cattcctaaa
     3301 gatttgctaa ttcctataac agaagttgat aattatccta aaaatgaagt gataattata
     3361 gctactggta tgcaaggaga acctgtagaa gccttaagtc aaatggcgca acataagcat
     3421 aaaattatga atatcgaaga aggcgattct gtatttttag caattacggc ttctgcaaat
     3481 atggaagtta tcattgcgaa tacattaaat gagcttgtac gtgctggcgc acatattatt
     3541 ccaaataaca agaagattca tgcttcaagt catggttgca tggaagaatt aaaaatgatg
     3601 attaatatta tgaaacctga atactttatt cctgtacaag gtgaatttaa aatgcagata
     3661 gcacatgcga agctagcagc tgaagcaggt gttgcaccag aaaagatttt ccttgtggaa
     3721 aaaggagatg tcattaatta caacggtaaa gatatgatat taaatgaaaa ggtaaattca
     3781 ggaaatattt taatagatgg tattggtatt ggggatgtag gaaatatcgt gttgagagac
     3841 cgtcatcttt tagcagaaga tggtatcttt attgctgtag taacgttaga tcctaaaaat
     3901 agacgtatag ctgcgggacc tgaaattcaa tctcgtgggt ttgtatatgt acgtgaaagt
     3961 gaagacttat tacgtgaagc agaagagaaa gtacgtgaaa tagtagaggc tggtttacaa
     4021 gaaaaacgca tagaatggtc tgaaattaag caaaatatgc gtgatcaaat tagtaaacta
     4081 ttattcgaaa gtacaaaacg tcgtcctatg attattccag taatttctga aatttaatca
     4141 aaaagtcatt aacataaaag aggtcagaac aagtcactga aatataatgg ttgtcatgga
     4201 caatttactt atattttatg atggtcaatt gaaaggggta acgattaatc tgttatctta
     4261 agtaaattga tacatagatg atattgttct aacctctttc atcgtctgtt tggactacat
     4321 attctaaaca tcaaatagga aattatatat aataacgtcg ttttaactaa ggcaacataa
     4381 ggaggtgcgt caattggcac aagcaaaaaa gaaatcgaca gctaagaaaa aaacaacatc
     4441 aaaaaaaaga acaaattcga ggaaaaagaa gaatgataat ccgatacgtt atgtcatagc
     4501 tattttagta gttgtattaa tggtgttggg tgttttccaa ttaggaataa taggtcgtct
     4561 aattgacagc ttctttaatt atttatttgg gtacagtaga tatttaacat atattttagt
     4621 actcttagca actggtttta ttacatactc taaacgtatt cctaaaacta gacgaacggc
     4681 tggttcgatt gtattgcaaa ttgcattgct atttgtatca cagttagttt ttcattttaa
     4741 tagtggtatc aaagctgaaa gagaacctgt actttcttat gtgtatcagt cataccaaca
     4801 cagtcatttc ccaaattttg gtggcggtgt attaggcttt tatttattag agttaagcgt
     4861 acctttaatt tcattatttg gtgtatgtat tattactatt ttattattat gctcaagtgt
     4921 tattttatta acaaaccatc aacatcgtga agtcgcaaaa gttgtactgg aaaatataaa
     4981 agcttggttt ggttcattta atgaaaaaat gtcggaaaga aaccaagaaa aacaattgaa
     5041 gcgtgaagaa aaagcgagac ttaaagaaga acaaaaggca cgtcaaaatg aacagccaca
     5101 aataaaagat gtgagtgatt ttacggaagt gcctcaagaa agagatattc caatttatgg
     5161 gcatactgaa aatgaaagta aaagtcagag tcaaccaagt cggaaaaagc gagtgtttga
     5221 tgcagagaat agttcgaata acatcgtaaa tcatcatcaa gcagatcagc aagaacaatt
     5281 aacagaacaa actcataaca gtgttgaaag tgaaaacact attgaagaag ctggtgaagt
     5341 tacgaatgta tcgtatgttg ttccaccgtt aactttactt aatcaacctg caaaacaaaa
     5401 agcaacatct aaagctgaag tgcaacgtaa aggacaagta ctagagaata cattaaaaga
     5461 ttttggggta aatgcaaaag tgacacaaat taaaattggt cctgcagtaa ctcaatatga
     5521 aattcaacca gctcaagggg ttaaagtgag taaaattgta aacttgcata atgatattgc
     5581 attagcttta gcagcaaaag atgttagaat cgaagcgcca atacctggtc gctctgcagt
     5641 aggtattgaa gtgccaaatg agaaaatttc attagtttca ctaaaagaag ttttagatga
     5701 aaaattcccg tctaataata aactagaagt tggattagga agagatatat caggtgatcc
     5761 aattactgtt ccactaaatg aaatgccaca cttattggtg gcaggatcga cgggtagtgg
     5821 taaatctgtt tgtataaatg gtattattac aagtatttta ttaaatgcta agccgcatga
     5881 agttaaactt atgttaatcg atccgaaaat ggttgaacta aatgtttata acggaattcc
     5941 acatttatta attccggttg ttacaaatcc tcataaagct gctcaagctt tagaaaaaat
     6001 tgtagctgag atggaaagac gttatgattt attccaacat tcatcaacta gaaatattaa
     6061 aggttataac gaattaatcc gtaagcaaaa tcaagaatta gatgagaagc aaccagaatt
     6121 accttatatc gttgttattg tagatgagct tgcagattta atgatggtag ctggtaaaga
     6181 agttgaaaat gcgattcaac gtattacaca aatggcacgt gcagcaggta tacatttaat
     6241 tgtagcgaca caaagacctt ctgtggatgt aattacaggt atcattaaaa ataatattcc
     6301 atctagaatt gcttttgctg taagttctca aacagattca agaactatta ttggtactgg
     6361 cggcgcagaa aagttacttg gtaaaggtga catgttatac gttggaaatg gtgattcatc
     6421 acaaacacgt attcaagggg cgtttttaag tgaccaagag gtgcaagatg ttgtaaatta
     6481 tgtagtagaa caacaacagg caaattatgt aaaagaaatg gaaccagatg caccagtgga
     6541 taaatcggaa atgaaaagtg aagatgcttt atatgatgaa gcgtatttgt ttgttgttga
     6601 acaacaaaag gcaagtacat cattgttaca acgccaattt agaattggtt ataatagagc
     6661 atctaggttg atggatgatt tagaacgcaa tcaggtaatc ggtccacaaa aaggaagcaa
     6721 gcctagacaa gttttaatag atcttaataa tgacgaggtg taaaaaaatg tcagaaatga
     6781 atgcggtata taacgttaaa caatacattt taaatttgat taagcaaaat aaattggaat
     6841 atggtgacca acttccaagt aatttatcaa ttgccagaga attaaatgta aaaaccgacg
     6901 atgtttatga agcaattcag gcattgatta ctgaacaagt cattaaagat aattttgaag
     6961 agggcacaag tgttaagtca ctgccccctt tcttttatcc attgaatgaa cttataagta
     7021 ttgggcaaat gattaaaaat gcaggatttg aatgcggaac tgaatactta aattttgatg
     7081 agcaaccagc aactatgtta gatgcaaatt tgttgagcgt tgaagaagga tatccagtaa
     7141 ccattataga acgattacga actgccgatg gagaaccggt cgtctattgt ttagataaaa
     7201 ttgctaaaaa agaattaaca tgtacagagt atcaaatgag caatggatcg atactaagtg
     7261 cgataaaaga acaaagtaat cataatattt gttacgctga tacagaaatt gaagcggtaa
     7321 attatgaacc tcgaatatcc gaagtactga atgcttcgcc acacgaaggt ttgattttat
     7381 taaaaattac gcactacaat gaattagatg aacctatttt gtattcatta aattatatga
     7441 aaaatagctt agttcaattt aaaatcacta gaaaaatata gatgtaagaa gatatagagg
     7501 aggctatata tttgagtaga caatctcaac caaatataca tatcaaagtt tcaccaacaa
     7561 ctaaatttaa aacaactact atagttttta aatttatggc acctttagaa tatgacacaa
     7621 ctacagctag atcattatta agcaaattat tagttcgagc aactaagaaa tggccaaccg
     7681 ataagtcgtt taataatcac ttagccgatt tatatggtgc atatgtgaat agtacaattt
     7741 caaaattcaa agatcagcat gtcattacat tttcattaga aattgtaaat gaacgttatt
     7801 taagaaacgg tgaatcatta tttaatcaag gattagattt attacaagaa atcatttgga
     7861 atccattaat tgaaaataaa gcattcaatg ataattttgt taaccaagag aaaacattat
     7921 tagccaaaaa aatagaagca atggtagata acaaagcaca atattcgttt ttaaaattac
     7981 tcgaccatat gtttgaaaat gaagcataca aatacttatc tacaggacaa ctagaacaaa
     8041 tcccacatat tactgctgaa acactatatc atacatatca atcaatgatt aataatgatc
     8101 aatgttctgt ttatgttgtc ggcaatgtag aacctgaaag tgttgagaaa caaatacgtg
     8161 aaaaatttgc acttaaacca ttcgataaac atcaattcca acattctaca catcatttac
     8221 acgatgaaga agttgattat attgttgaat atgatgacgt ggatcaagct aaattaaata
     8281 tgggatatcg ttttccaaca caatatggac aaagtggata tgctgctttt gttgtattta
     8341 acatgatgtt tggaggagat ccttcatctg ttttatttaa tgaagtgcga gaaaagcaaa
     8401 gtttagcgta ctctatacat tcacaaattg atggcaaaaa tggctattta tttgttttga
     8461 gtggggtttc aagtgataag tacgaaactg caaaagacac tattataagt gaatttgaaa
     8521 aaataaaagc aggagatttc actgaagaaa aattagagtt agctaaaaaa gtaatcattt
     8581 ctcatcgata tgaatctgaa gatcgtccga aaagtattat agagattatg cataaccaaa
     8641 tattattaga gcaaccacaa agcaaagaaa catttataaa tgatatacag aaggtaagtc
     8701 gcgaagatat tgtttctgtt gctgaaaaag catttttaga tacaatctat gtgttgacaa
     8761 aaggagggga taaataatga aagagcgtta ttatgaatta atagacgaaa gagtattcga
     8821 acaagaatta gaaaatggtt tacgattatt tgttatccct aaaccaggtt ttcaaaagac
     8881 atttgtcact tacactacac aatttggttc attagataat caattcaaac ccctcggaca
     8941 agaccaattt gttactgtac ctgatggagt tgctcacttt ttagaacata aattatttga
     9001 aaaagaagaa gaagacttat ttactgcatt tgctgaagat aatgcacaag caaatgcgtt
     9061 tacaagcttt gatcgtacaa gctacttgtt cagtgcaact gataatattg aaaacaacat
     9121 taaacgttta cttacaatgg ttgaaacgcc ttattttaca aaagaaactg ttgataaaga
     9181 aaaaggtatt attgcagaag aaataaaaat gtatcaagag caacctggat ataaattaat
     9241 gtttaataca ttgcgtgcaa tgtatcaaca acatccaata cgtgttgata ttgccggtag
     9301 tgtagaaagt atatacgata ttacaaaaga tgatttgtat ctatgttatg aaacgtttta
     9361 tcatccatca aatatggttt tatttgttgt tggcgatgtg gatcctgaag caatatgtcg
     9421 aatagtaaaa caacacgagg atgctcgtaa taaagttaac caacccaaaa tcgaacgagg
     9481 acttgttaat gaaccggagg atgttaaaga agcatttgtt actgaatcta tgaaaattca
     9541 atcaccaaga ctaatgcttg gttttaaaaa taaaccatta caagaagcgc ctcaaaaata
     9601 tgtacaacgt gatttagaaa tgtcattatt ctttgagtta atttttgggg aagaaacaga
     9661 tttttatcag aatttattaa acgaaggact tatcgatgat acatttggtt atcaatttgt
     9721 actagagccg acttatagtt tttcaatcgt gacaagcgct actgaagaac cagataaatt
     9781 aaaaaaatta ttattagatg agttgcgtga taaaaaaggc aatttccaag atgcagaagc
     9841 atttgaactt ttgaaaaagc aatttatagg tgaattcata tcaagtttaa actcacctga
     9901 atatattgct aatcaatata ctaaattgta ttttgaaggt gttagcgtgt ttgacatgtt
     9961 agacattgtt gaaaatatca ccttagatag tattaacgaa acgtcatcgt tatatttaaa
    10021 tttagatcag caagtcgata gtcgtttgga gattaaaaag taatgaaagc attagtatta
    10081 ggtggttctg gttcaattgg ttctgagata gtcaaacaat tattaactga tggatttgaa
    10141 gtttatgtgc aatattatcg tactgatata aatgaattaa ctagcaaatt taatgatgat
    10201 aaagttcgtt ttatacaagc ggatttatct caaacaattg atattgacaa aacatttggt
    10261 gacattaaat cattagactg tttaatatat gcaagtggtc agtctttata tggtgtttta
    10321 caagatatga aagaccatga tattgatgca tgttatcagt taaatgtctt gcaattaatt
    10381 cgattatgta gatatttcgt tgatgtttta cgtcaaagtg acaatggaag aattattgta
    10441 atttcatcaa tttggggtga gacaggagct agtatggaaa ctatttattc gacgatgaaa
    10501 agtgcacaat taggtttcgt taaggcgctt agtcaagagc ttgcactaac atcagtgaca
    10561 gtaaatgcta tcgcacctgg atttgtagcc ggtaatatgg caagtgagtg gcaagaagat
    10621 gaacttcaag caatgataac tgaattacca caacagcgat tgattttacc gagtgaggtt
    10681 gctcatacat gcgcctattt atatcaccca aatgctagaa gtgttactgg aactatacag
    10741 aaagttaatg gtgcttggta tatttaaaat atagaactat ttttatgagc aaatgttttt
    10801 atgattgatg ttataatatg aatattacaa tgaatcataa tttgttaatt aaacaagggg
    10861 tgaggttcac catgacagtt gcagagaaaa aagaatggta cctagaatac gaaattgaaa
    10921 ttaatagacc gggtctttta ggtgatgtat ctagtttatt aggtatgtta ggtataagta
    10981 ttgttacaat taatggtgtt gatcaaggta aacgaggcct tttaattaaa acagacaatc
    11041 ttgaaaaagt tgaacgtttt gagcaaatag ctcgtggtat aaatgaaatt gaaataacaa
    11101 agcttaaaaa accagaatta agagaccgtc ttgcagtaag acatggtaga tatattgagc
    11161 aagatgcaaa agataagaaa acctttcgat ttgagcgtga agatttaggc ttgttagtag
    11221 actttttagc tgaattgttc aaagaagaag gtcataagtt gattggcatt agaggtatgc
    11281 cacgagttgg taaaactgaa tcaattgttg cgggaagtgt ttgtgcacat aagagatggt
    11341 tatttattag ttctacttta ataaaacaaa ctgtacgtag ctctctaatt aaaggggaat
    11401 atgatgccaa tcatgtatac attattgatg gtgcagttac tgccagagaa tctaatccaa
    11461 aacatcaaga gcttgttaac gaagttatga cgttaccatc aatcaaagtc gttgaacatc
    11521 cagatttatt tgttgaaaca agtacttgta caatggaaga ttttgactat attatcgaat
    11581 tgagagaaaa tgaaaatcaa gaaatacatt acgaagaaat gaagaaacag acagtccaaa
    11641 gtaagaataa tttagatttt ggagatccgt ttggtggtgg ttttggtttc ttcgagtaag
    11701 tgtaaggagg ctatgaattg aaaacggtcg gtgaagcgct aaaaggtaga cgtgaaaggt
    11761 taggaatgac tttaacagaa ttagagcaac gtactggaat taaacgtgaa atgctagtgc
    11821 atattgaaaa taatgaattc gatcaactac cgaataaaaa ttacagcgaa ggatttatta
    11881 gaaaatatgc aagcgtagta aatattgaac ctaaccaatt aattcaagct catcaagatg
    11941 aaattccatc gaaccaagcc gaatgggacg aagtaattac agttttcaat aataataaag
    12001 acttagatta taagagtaaa tcaaaagagc caatacaatt attagtaatc atgggtatta
    12061 cagttttaat aactttattg ttatggatca tgttagtttt aatattttaa cagaaataaa
    12121 ttagtgagaa atgaggatgt ataatgaata ttccgaacca gattacggtt tttagagtag
    12181 tgttaatacc agtttttata ttgtttgcgt tagttgattt tggatttggc aatgtgtcat
    12241 ttctaggagg atatgaaata agaattgagt tattaatcag tggttttatt tttatattgg
    12301 cttcccttag cgattttgtt gatggttatt tagctagaaa atggaattta gttacaaata
    12361 tggggaaatt tttggatcca ttagcggata aattattagt tgcaagtgct ttaattgtac
    12421 ttgtgcaact aggactaaca aattctgtag tagcaatcat tattattgcc agagaatttg
    12481 ccgtaactgg tttacgttta ctacaaattg aacaaggatt cgtaagtgca gctggtcaat
    12541 taggtaaaat taaaacagca gttactatgg tagcaattac ttggttgtta ttaggtgatc
    12601 cattggcaac attgattggt ttgtcattag gacaaatttt attatacatt ggcgtta
//
LOCUS       DAMZKC010000072        12612 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.072, whole genome shotgun sequence.
ACCESSION   DAMZKC010000072 DAMZKC010000000
VERSION     DAMZKC010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12612
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.072"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(481..1821)
                     /gene="glnA"
                     /locus_tag="POB55_001474"
     CDS             complement(481..1821)
                     /gene="glnA"
                     /locus_tag="POB55_001474"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HDI6500440.1"
                     /translation="MPKRTFTKDDIRKFAEEENVRYLRLQFTDILGTIKNVEVPVSQL
                     EKVLDNEMMFDGSSIEGFVRIEESDMYLHPDLDTWVIFPWTAGQGKVARLICDVYKTD
                     GTPFEGDPRANLKRVLKEMEDLGFTDFNLGPEPEFFLFKLDEKGEPTLELNDDGGYFD
                     LAPTDLGENCRRDIVLELEDMGFDIEASHHEVAPGQHEIDFKYADAVTACDNIQTFKL
                     VVKTIARKHNLHATFMPKPLFGVNGSGMHFNVSLFKGKENAFFDPNTEMGLTETAYQF
                     TAGVLKNARGFTAVCNPLVNSYKRLVPGYEAPCYIAWSGKNRSPLIRVPSSRGLSTRI
                     EVRSVDPAANPYMALAAILEAGLDGIKNKLKVPEPVNQNIYEMNREEREAVGIQDLPS
                     TLYTALKAMRENEVIKKALGNHIYNQFINSKSIEWDYYRTQVSEWERDQYMKQY"
     gene            complement(1840..2208)
                     /locus_tag="POB55_001475"
     CDS             complement(1840..2208)
                     /locus_tag="POB55_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDI6500441.1"
                     /translation="MISNDAIRRNMAVFSMSVVSKLTDLTPRQIRYYETHELIKPERT
                     EGQKRLFSLNDLERLLEIKSLLEKGFNIKGIKQIIYDSQEHLTTDEQEIRKKMIVDAT
                     QKPIGETLPINRGDLSRFIK"
     gene            complement(2452..3690)
                     /locus_tag="POB55_001476"
     CDS             complement(2452..3690)
                     /locus_tag="POB55_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDI6500442.1"
                     /translation="MKDISKIVADVESTLAPYFKEIEETAYINQEKVLNAFHHVKATE
                     SDLQGSTGYGYDDFGRDHLEEIYAQAFKAEDAIVRPQIISGTHAITIALQSLLKHGDE
                     LIYITGSPYDTLLEVIGVNGNGIESLMEHGVSYKDIALKEGKIDIESVLDGVSERTKV
                     IAIQRSKGYDQRPSIPLDEIEQVITRLKEVHPNILIFVDNCYGEFVERREPIECGADL
                     IAGSLIKNPGGGLAKIGGYIAGRKDLIERCGYRLTAPGIGKEAGASLNALLEMYQGFF
                     LAPHVVSQSLKGALFTSLFLEKMNMNTTPKYYEKRTDLIQTVKFKTKEQMISFCQSIQ
                     HASPINAHFSPEPSYMPGYEDDVIMAAGTFIQGSSIELSADGPIRPPYEAYVQGGLTY
                     EHVKIAVTRAVNQLKEQGLI"
     gene            complement(3709..4947)
                     /gene="hflX"
                     /locus_tag="POB55_001477"
     CDS             complement(3709..4947)
                     /gene="hflX"
                     /locus_tag="POB55_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDI6500443.1"
                     /translation="MAQQQIHDTKNKLEKAVLVGVHAQDDKQFNFESTMEELSSLSET
                     CQLEVLGQITQNRDRVDRKYYVGKGKMEEIQAFIEFKDIDVVITNDELTTAQSKSLNE
                     ALGVKIIDRTQLILEIFALRARSKEGKLQVELAQLDYLLPRLQGHGKSLSRLGGGIGT
                     RGPGETKLEMDRRHIRTRMNEIKHQLRTVEEHRERYRNKRNQNQVFQVALVGYTNAGK
                     SSWFNVLANEETYEKDQLFATLDPKTRQIQINDGFNLIISDTVGFIQKLPTTLIAAFK
                     STLEEAKGADLLVHVVDSSHPEYRTQYDTVNDLIKQLDMSHISQIVIFNKKDLCDHAS
                     NCPASDLPNVFVSSKNDGDKLLVKTLFIDEIKRQLTYYDETIATNNADRLYFLKQHTL
                     VTELKYDEIENVYRIKGFKK"
     gene            5059..5535
                     /locus_tag="POB55_001478"
     CDS             5059..5535
                     /locus_tag="POB55_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HDI6500444.1"
                     /translation="METIYDFVVETNKGVTYKLDAYKGDVMLIVNTASECGFTSQFEG
                     LQSLYEKYKDQGFVILGFPCNQFGGQEPGSGEEAAQNCKLNYGVTFPMHQKIDVKGEH
                     QLPLFRYLTAAQHGFFNEKIKWNFTKFLVDREGNVVKRFAPQKKPVQIEREIEKLL"
     gene            complement(5757..5990)
                     /gene="hfq"
                     /locus_tag="POB55_001479"
     CDS             complement(5757..5990)
                     /gene="hfq"
                     /locus_tag="POB55_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HDI6500445.1"
                     /translation="MIANENIQDKALENFKANQTEVTVFFLNGFQMKGVIEEYDKYVV
                     SLNSQGKQHLIYKHAISTYTVETEGQASTESEE"
     gene            complement(6005..6940)
                     /gene="miaA"
                     /locus_tag="POB55_001480"
     CDS             complement(6005..6940)
                     /gene="miaA"
                     /locus_tag="POB55_001480"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HDI6500446.1"
                     /translation="MNKNKPFIVVIVGPTASGKTELSIELAKRINGEIISGDSMQVYK
                     HMNIGTAKVTPEEMDGIPHHLIDILNPDDTFSAYEFKRLAEDLITDITNRGKVPIIAG
                     GTGLYIQSLIYNYELEDETVTPAQLSIVKQKLSALEHLDNQQLHDYLAQFDAVSAENI
                     HPNNRQRGLRAIEYYLKTKKLLSNRKKVQQFTENYDTLLLGIEMSRKTLYSRINKRVD
                     IMLDHGLFREVQQLVEQGYESCQSMQAIGYKELIPVINGQMIYEDAVNDLKQHSRQYA
                     KRQMTWFKNKMSVHWLDKENMSLQMMLDEITTQIK"
     gene            complement(6958..7872)
                     /locus_tag="POB55_001481"
     CDS             complement(6958..7872)
                     /locus_tag="POB55_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDI6500447.1"
                     /translation="MTEKQFKLTVQDNTNIEVKVNFTDVDSKGIIHIFHGMAEHMERY
                     DKLAHALSKHGFDVIRHNHRGHGINIDESTRGHYDDMKRVIGDAFEVAQTVRGNVDKP
                     YIIIGHSMGSVIARLFVETYPQYVDGLILSGTGMYSLWKGLPTVKVLQLITKIYGAEK
                     RVEWVNQLVSNSFNKKIRPLRTQSDWISSNPIEVDNFIKDPYSGFNVSNQLLYQTAYY
                     MLHTSQLKNMKMLNHAMPILLVSGYDDPLGDYGKGILKLANIYRKAGIKNVKVNLYHH
                     KRHEVLFEKDHDKIWEDLFKWLNQFYKK"
     gene            complement(8022..9695)
                     /locus_tag="POB55_001482"
     CDS             complement(8022..9695)
                     /locus_tag="POB55_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HDI6500448.1"
                     /translation="MALSTFKREHIKKNLRNDEYDLVIIGGGITGAGIALDASERGMK
                     VALVEMQDFAQGTSSRSTKLVHGGLRYLKQFQIGVVAETGKERAIVYENGPHVTTPEW
                     MLLPMHKGGTFGKFSTSIGLGMYDRLAGVKKSERKKMLSKKETLAKEPLVKKEGLKGG
                     GYYVEYRTDDARLTIEVMKRAAEKGAEIINYTKSEHFTYDKNQQVNGVKVIDKLTNEN
                     YTIKAKKVVNAAGPWVDDVRSGDYARNNKKLRLTKGVHVVIDQSKFPLGQAVYFDTEK
                     DGRMIFAIPREGKAYVGTTDTFYDNIKSSPLTTQEDRDYLIDAINYMFPSVNVTDEDI
                     ESTWAGIRPLIYEEGKDPSEISRKDEIWEGKSGLLTIAGGKLTGYRHMAQDIVDLVSK
                     RLKKDYGLTFSPCNTKGLAISGGDVGGSKNFDAFVEQKVDVAKGFGIDEDVARRLASK
                     YGSNVDELFNIAQTSQYHDSKLPLEIYVELVYSIQQEMVYKPNDFLVRRSGKMYFNIK
                     DVLDYKDAVIDIMADMLDYSPAQIEAYTEEVEQAIKEAQHGNNQPAVKE"
     gene            complement(9853..11349)
                     /gene="glpK"
                     /locus_tag="POB55_001483"
     CDS             complement(9853..11349)
                     /gene="glpK"
                     /locus_tag="POB55_001483"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDI6500449.1"
                     /translation="MEKYILSIDQGTTSSRAILFNQKGEIAGVAQREFKQYFPQSGWV
                     EHDANEIWTSVLAVMTEVINENDVRADQIAGIGITNQRETTVVWDKHTGRPIYHAIVW
                     QSRQTQSICSELKQQGYEQTFRDKTGLLLDPYFAGTKVKWILDNVEGAREKAENGDLL
                     FGTIDTWLVWKLSGKAAHITDYSNASRTLMFNIHDLEWDDELLELLTVPKNMLPEVKP
                     SSEVYGKTIDYHFYGQEVPIAGVAGDQQAALFGQACFERGDVKNTYGTGGFMLMNTGD
                     KAVKSESGLLTTIAYGIDGKVNYALEGSIFVSGSAIQWLRDGLRMINSAPQSESYATR
                     VDSTEGVYVVPAFVGLGTPYWDSEARGAIFGLSRGTEKEHFIRATIESLCYQTRDVME
                     AMSKDSGIDVQSLRVDGGAVKNNFIMQFQADIVNTSVERPEIQETTALGAAFLAGLAV
                     GFWESKDDIAKNWKLEEKFDPKMDEGEREKLYRGWKKAVEATQVFKTE"
     gene            complement(11478..12296)
                     /locus_tag="POB55_001484"
     CDS             complement(11478..12296)
                     /locus_tag="POB55_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HDI6500450.1"
                     /translation="MNVYLAEFLGTAILILFGGGVCANVNLKRSAANGADWIVITAGW
                     GLAVTMGVFAVGQFSGAHLNPAVSLALALDGSFDWSLVPGYIVAQMLGAIVGATIVWL
                     MYLPHWKATEEAGAKLGVFSTAPAIKNYFANFLSEIIGTMALTLGILFIGVNKIADGL
                     NPLIVGALIVAIGLSLGGATGYAINPARDLGPRIAHAILPIAGKGGSNWSYAIVPILG
                     PIAGGLLGAVVYAVFYKHTFNIGCAIAIVVVIITLILGYILNKSSKKGDIESIY"
BASE COUNT         3895 a         2450 c         1754 g         4513 t
ORIGIN      
        1 taatttttta gaatatcaat agtagtataa ctaaaatgaa aataccgatc gataaacaaa
       61 aagatatttt ttgttttgtt tctcttttca tatagtatta cccccttaat aatgagtagt
      121 aaggtccctc ttttcggcgt cttaccttag aacgttctgc aaatgaattc gatgagaagt
      181 aatatgatat ggctattttc aagtaatact caacgttttc gcgacgttct tttatcgcct
      241 catctcatca cctccaaata tattaaaatt catgtgaact aaaatataaa atggtcttcc
      301 ccagctttaa aaaaataaat acacaaaaca tttacttgac caaaacttga ccaaaagttg
      361 cccaatcgta acaaaaggca cattttacac ccaaaaaagt tgcccagtta aatcaaaaaa
      421 gagctaaagc aaaacgctct agcccttgat attactgatt tcccatcatt gtttagcgta
      481 ttaatattgc ttcatgtact gatctctttc ccattcagat acttgagttc tgtagtaatc
      541 ccattcaatt gattttgaat taataaattg attatagata tgatttccta aagctttttt
      601 tataacttca ttttcacgca ttgcttttaa tgctgtgtaa agtgttgaag gtaagtcttg
      661 aatgcctact gcttcacgtt cttcacgatt catttcgtaa atattttggt taacaggttc
      721 tggaactttt aatttatttt taataccgtc taaaccagct tctaaaattg cagctaacgc
      781 catatatggg ttcgcagctg ggtcaactga acgtacttca atacgagttg ataatcctct
      841 tgaagatggt acacggatta acggtgaacg gtttttaccg ctccatgcaa tataacatgg
      901 tgcttcataa ccaggaacta aacgtttgta tgagtttact aatgggttac ataccgctgt
      961 aaatccacgt gcgtttttaa gtacaccagc tgtgaattgg taagctgttt ctgttaagcc
     1021 catttcagta ttcggatcaa agaatgcatt ttctttacct ttgaataatg aaacgttaaa
     1081 gtgcataccg ctaccattca caccaaataa tggtttaggc ataaatgttg cgtgtaaatt
     1141 atgtttacgt gcgattgttt taacaaccaa tttaaatgtt tggatattat cacatgctgt
     1201 aacagcatct gcatatttaa agtcaatttc atgttgacca ggggcaactt catggtgact
     1261 agcttcaata tcgaagccca tatcctctaa ttctaaaaca atatcacgac gacagttttc
     1321 acctaaatct gtaggtgcta aatcgaaata tccaccatca tcattaagtt ctaaagttgg
     1381 ttcccctttt tcatccaact taaacaagaa gaattctggt tcaggcccta ggttaaagtc
     1441 tgtgaagcct aaatcttcca tttcttttaa tacacgtttt aagtttgcac gaggatcccc
     1501 ttcaaatggt gttccatctg ttttatatac atcacaaatt aaacgtgcaa ctttaccttg
     1561 tccagcagtc catgggaaga ttacccaagt atctaaatct ggatgtaagt acatatctga
     1621 ttcttcgata cgtacgaaac cttcgataga agaaccgtca aacatcattt cgttatcaag
     1681 tactttttct aattggctta caggcacttc aacattttta attgttccta aaatatcagt
     1741 gaattgtaat cttaaatatc ttacattttc ctcttctgca aatttacgaa tgtcgtcttt
     1801 agtgaaagta cgttttggca ttttaaaatc ctccaaattt tatttaataa atcgggataa
     1861 atcaccacga tttattggca aagtttctcc aataggcttt tgcgtggcat ctacaatcat
     1921 cttttttctt atctcttgtt catctgttgt taaatgctct tgtgagtcat aaatgatttg
     1981 tttaatccct ttgatattaa atcctttttc taataatgat ttaatttcta gtaatctttc
     2041 caaatcattg agtgagaaca gacgtttttg accttctgtt ctttcaggtt tgatgagttc
     2101 atgtgtttca tagtaacgta tttgccttgg cgttaaatcc gttaacttac ttactacact
     2161 catagagaag acagccatat ttcgtctgat tgcatcattc gatatcattg ttcctctcct
     2221 ctacttttga acttgttaat aacttatcat aattttttta ggatttcaaa aatatgtcag
     2281 gttttctgac ataaagctca aaacattgat ataactgcaa ttttgacctt taaaacgcca
     2341 taaaacaatc tacagctaaa atattttttc aatttttaaa aataatctga taaatctccg
     2401 caaaaaaatt tcacccaaaa cacattatca tttagggtga aatttaatct attatataag
     2461 tccttgttct ttcaactgat taacagctct tgtcacagca attttaacgt gttcatatgt
     2521 taatcctcct tgaacatatg cttcataagg aggacgaata ggtccatctg cagataattc
     2581 aatcgatgaa ccttgaataa acgtaccagc agccataata acatcatctt cgtaaccagg
     2641 catataactc ggttctggac taaaatgtgc attaattggg gatgcgtgtt gaatactttg
     2701 acaaaatgaa atcatttgtt ctttcgtttt aaatttaact gtttgaatta aatcagttcg
     2761 tttttcgtag tacttcggcg ttgtgttcat attcattttt tctaaaaata aactagtaaa
     2821 caatgcccct ttaagactct gactgacaac atgtggtgct aaaaagaaac cttgatacat
     2881 ttcaagcaat gcatttaatg atgcacctgc ttctttacca ataccaggtg ctgtcaatct
     2941 ataaccacat cgttcaatta aatcttttct accagcaatg tatccaccaa tcttagctaa
     3001 accaccgcca gggtttttaa ttaatgatcc tgctattaaa tcggcaccac attctatagg
     3061 ttcacgtctt tcaacaaatt ccccataaca gttatccaca aatattaaaa tattaggatg
     3121 cacttctttc aacctagtaa tcacctgttc aatttcatct agaggaattg aaggtctttg
     3181 atcatagcct ttcgaacgtt gaatcgctat tactttggta cgctcagaaa ccccatctaa
     3241 cacactttca atatcgatct taccttcttt aagtgcaata tctttatacg atacaccgtg
     3301 ctccattaaa ctttcaatac catttccgtt tacaccaatg acttcaagta aagtgtcata
     3361 tggacttccc gttatataaa ttaattcatc accatgtttt aataaacttt gtaatgcaat
     3421 agtaatcgca tgcgtacctg aaataatttg cggacgaaca attgcatctt ctgctttaaa
     3481 tgcctgcgca tatatttctt ctaaatgatc acgtccaaaa tcatcatatc cgtatcctgt
     3541 tgatccttgt agatcacttt cggttgcttt gacatgatga aatgcattta atactttttc
     3601 ttgattaata tatgctgttt cttcaatttc tttaaaatat ggtgctaacg ttgattcgac
     3661 gtcagctact atcttactta tatctttcat ttgaatttcg tccttttatt attttttaaa
     3721 tccttttata cgataaacat tttcaatttc atcatattta agttcagtca ctaatgtatg
     3781 ttgttttaga aaatataatc gatctgcatt attcgtcgca attgtctcat cataataagt
     3841 taattgcctt ttgatttcat caataaataa cgtcttaaca agtaatttat caccatcatt
     3901 tttagaagaa acaaaaacat taggcaaatc acttgctgga caatttgatg catgatcaca
     3961 taagtccttt ttattaaaaa taactatttg agaaatatga ctcatatcta attgtttgat
     4021 taaatcatta actgtgtcat actgcgtacg gtattcagga tggctactat ctacgacatg
     4081 tactaataaa tctgcacctt tagcctcttc taaagttgat ttaaaagctg caattaacgt
     4141 cgtaggtagt ttctgtataa aaccaacagt atctgaaata attaaattaa atccatcatt
     4201 tatttgaatt tgtcgtgttt taggatctaa cgttgcaaat aattgatctt tttcatatgt
     4261 ctcttcattt gctaaaacat taaaccatga tgatttacca gcatttgtat aaccaactaa
     4321 agctacttga aacacctgat tttgatttct tttatttcga tatctttcgc gatgttcttc
     4381 taccgtccgc aattgatgtt taatttcatt catacgagtt cgaatatgtc tgcgatccat
     4441 ctctaacttc gtttcaccag ggcctctagt tccaataccg ccacctaaac gagaaagact
     4501 tttaccatgg ccttgcaatc taggtaataa ataatcaagt tgtgctagct ctacttgcaa
     4561 tttaccttct ttacttcttg ctcttaatgc aaatatttca agaatcaact gagttctatc
     4621 aataattttt acacctaaag cttcatttag tgatttggat tgtgcagtcg ttaattcatc
     4681 attcgtgatg actacatcaa tatctttgaa ctcaataaat gcttgaattt cttccatttt
     4741 acctttacca acataatatt tgcgatctac acgatctctg ttttgagtaa tttgacccaa
     4801 cacttcaagt tggcaagtct ctgataaaga tgataattcc tccattgtag actcaaaatt
     4861 aaattgctta tcatcttgag catgtacacc gactaagaca gctttttcta gtttattttt
     4921 agtatcatga atttgttgct gagccatgtt atacatctcc ttaataaaat cctactcaaa
     4981 gtattctatc atagagtttt agtaaagact attacacgat tttatggtac attttaagta
     5041 aatcgaaagg atgattcaat ggagacaatt tatgattttg tagtagaaac aaataaaggt
     5101 gttacttaca aattagatgc atataagggt gacgttatgc taattgttaa tacagctagc
     5161 gaatgtggct ttacttcaca atttgaaggt ttgcaatcat tatacgagaa atacaaagat
     5221 caagggtttg taattctagg tttcccttgt aatcaatttg gtggccaaga acctggatca
     5281 ggcgaagaag cagctcaaaa ctgtaaatta aactatggtg ttaccttccc tatgcatcaa
     5341 aaaatagacg taaaagggga acatcaatta cctttattta gatatttgac tgcggcacaa
     5401 cacggattct ttaatgagaa aatcaaatgg aatttcacta agtttttagt ggatcgcgaa
     5461 ggtaacgttg ttaaacgatt tgcacctcaa aaaaaacctg ttcaaatcga aagagaaata
     5521 gaaaaattat tataaaatat actagctcat agcaaataaa acgcttcata taaagtcgta
     5581 tcatatattg actcgacctt tatatgaagc gtttttatta caaacattcc tctattgtat
     5641 cttgtcattt gagcatcatt taattgatgt cacatatgac tttatttcta tatctattta
     5701 tcatacttca aacgttatgt acacgagtca ttaaatcgtg cccatatgat tacaacttat
     5761 tcttcacttt cagtagatgc ttgaccttca gtttctactg tataagtgct gatcgcatgt
     5821 ttgtaaatca agtgttgttt gccttgagaa tttaagctta cgacatactt gtcgtattct
     5881 tcaataacac ctttcatttg gaaaccgttt agaaagaata cagttacttc agtttggttt
     5941 gctttaaaat tctctagtgc tttgtcttgg atgttttcgt ttgcaatcat ctgtcggact
     6001 ccttttactt aatctgggtt gtaatctcat ctaacatcat ttgaagtgac atattttctt
     6061 tatctaacca atgaacactc attttattct tgaaccatgt catttgtcgt tttgcatatt
     6121 ggcgtgaatg ttgctttaaa tcattgacag catcttcata aatcatttgt ccgttaatca
     6181 caggtattaa ttctttatat ccaatagctt gcatactttg gcaagattca tagccttgtt
     6241 caacaagttg ttgcacttct ctaaataatc cgtgatccaa cataatatca acacgtttat
     6301 ttattcttga atataatgtt ttacgcgaca tttcaatccc taataataat gtatcataat
     6361 tttcagtaaa ttgttgcact ttcttgcgat tactcaaaag tttttttgtt tttaaataat
     6421 attcaatagc gcgcaaccct ctttggcggt tgttagggtg aatattttct gcagaaaccg
     6481 catcaaattg agctaaataa tcgtgtagtt gctgattatc taaatgttct aatgcagata
     6541 acttttgttt aactatggat aattgtgcag gtgtaactgt ttcatcttct aattcataat
     6601 tatatattaa tgattgaata tataagcctg ttccacctgc tatgattgga actttacctc
     6661 tattcgttat atcagtaatt aaatcttctg ctaatcgctt gaattcatat gctgaaaatg
     6721 tatcatcagg attcaagata tcaattaaat gatgtggaat accatccatt tcttcaggtg
     6781 ttacttttgc agttccaata ttcatatgtt tgtaaacttg catagaatca ccgcttatga
     6841 tttcaccatt gatacgcttc gcgagttcta tgctaagctc tgttttacct gaagcagttg
     6901 gccccacaat tactacaata aaaggcttat ttttattcat atttaattcc actttcttta
     6961 ttttttataa aattgattca accatttaaa caagtcttcc caaattttgt catgatcttt
     7021 ttcaaataac acttcatgac gtttatgatg ataaagattc actttaacat ttttaatgcc
     7081 agcttttcta tatatattcg ccaattttaa aatcccttta ccataatcac ctaaaggatc
     7141 gtcatatcct gaaactaata atataggcat ggcatgattt aacattttca tattttttaa
     7201 ttgtgatgta tgtagcatat aataggctgt ttgatataat aattgatttg acacattaaa
     7261 tccactatat ggatctttaa taaagttatc tacttcaatt ggattactag aaatccaatc
     7321 actttgtgta cgtaatggac gtattttttt attaaaacta tttgatacta actggttaac
     7381 ccattcaact cgtttctcag caccataaat ttttgtaatc agttgtaaca ctttaacggt
     7441 tggtaaacct ttccataatg aatacatacc agtaccactt aaaattagac catcaacata
     7501 ttgcggatat gtttctacaa acaatctagc tataacggat cccattgaat gtccgattat
     7561 aatgtatggt ttatcaacat tgcctctcac tgtttgcgct acttcaaagg catcaccgat
     7621 aactcgtttc atatcatcgt aatgccctct tgttgattca tcaatattaa taccatgtcc
     7681 tcgatgatta tgacgtatca catcgaagcc atgctttgaa agtgcatgtg ctaatttatc
     7741 gtaacgttcc atatgttcag ccataccatg aaatatatga ataattcctt ttgaatctac
     7801 atctgtaaaa ttcactttaa cttcaatatt cgtattatct tgtacagtta atttaaattg
     7861 tttttcagtc atcatctcac acctttaata acaacgcttt tatattcgct tttattatta
     7921 tcatacattt agtgatgcat agtttcaatt gactaatgta tctcgtataa aaaaactgat
     7981 gcccttaaaa caggacacca gtttatgatt gtacaaatta attattcttt aactgctggt
     8041 tgattatttc catgttgcgc ttctttaatt gcttgctcaa cttcttcagt atatgcttca
     8101 atttgagctg gagagtaatc aagcatatct gccataatat cgatgacagc atctttataa
     8161 tctaatacat ctttaatatt gaaatacatt ttaccagaac gacgaactaa gaaatcgtta
     8221 ggtttgtata ccatttcttg ttgaatacta taaacaagtt ctacataaat ttctaatggt
     8281 aacttgctat catggtattg agatgtttgc gcaatgttga acaattcatc aacatttgaa
     8341 ccatatttag atgctaaacg tcttgcaaca tcttcatcaa tgccgaatcc tttagctaca
     8401 tctacttttt gctctacaaa cgcatcaaag ttcttgctac cacctacatc gccacctgaa
     8461 attgccagac cttttgtatt acatggacta aatgttaaac cgtagtcttt tttcaagcgt
     8521 ttagatacta aatcaacaat gtcttgagcc atgtgacgat agcctgttaa tttaccacct
     8581 gcaatagtta ataaacctga tttaccttcc caaatttcat ccttacgaga gatttcagaa
     8641 gggtctttgc cttcttcgta aattaatggt ctaattcctg cccatgttga ttcaatatct
     8701 tcatctgtaa cattaacact agggaacatg taattaatcg catcgattaa atagtctctg
     8761 tcttcttgtg tagttaatgg tgaagatttg atattgtcat agaatgtatc tgtagtacct
     8821 acatacgctt ttccttcacg tggaattgca aaaatcattc ttccatcttt ttcagtatca
     8881 aagtatactg cttgacctaa tgggaatttt gattgatcaa taacaacatg tacaccttta
     8941 gttaaacgta attttttatt attgcgtgca taatcaccac ttctaacatc atcaacccat
     9001 ggacctgctg cattaaccac ttttttagcc ttaattgtat aattttcatt agttaattta
     9061 tctataactt taacaccatt tacttgttga tttttatcat aagtgaagtg ttcagattta
     9121 gtatagttga taatttctgc gcctttttca gcagcacgct tcataacttc aatagttaaa
     9181 cgcgcatcgt cagtacgata ttcaacatag taaccgccgc cttttagacc ttctttttta
     9241 actaatggtt ctttagctaa agtttctttt ttagataaca tttttttacg ttcagacttc
     9301 ttaacacctg ctaaacgatc atacattcct aaaccaattg atgttgagaa tttaccaaat
     9361 gttccacctt tatgcattgg taaaagcatc cactctggag tcgtaacatg aggtccattt
     9421 tcataaacaa tcgcacgttc tttaccagtt tcggcaacta ctccaatttg gaattgtttt
     9481 aagtaacgta aaccaccatg gactaatttt gtagatcttg agcttgttcc ttgtgcaaag
     9541 tcttgcattt caactaatgc aactttcatt cctctttcac tcgcgtctag tgcaatacct
     9601 gcacctgtaa taccgccacc aataattact aaatcatatt catcatttct taaattcttt
     9661 ttaatatgtt ctctcttaaa agtagacaac gccattccaa aacgcctcct aaaaaatgta
     9721 aaaacgagag acttgtccca cataaacaat ccttcaattg tacatgttcg aacaaatctc
     9781 tcgcttctcg attcaaatat taacttaaat tgattgtatc acaatgttta tacaaaagtc
     9841 taatctacaa gtttattctg ttttaaaaac ttgtgttgct tcaacagctt ttttccaacc
     9901 tctatataat ttttctcttt cgccttcatc cattttcgga tcgaattttt cttctaattt
     9961 ccagttttta gcgatatcat ctttactctc ccagaatcca actgctaatc ctgctaaaaa
    10021 tgcagcacct aaagctgtag tttcttgaat ttcaggtctt tcaacagaag tattaacaat
    10081 gtctgcttgg aactgcataa taaagttgtt tttaactgca ccaccatcga cacgtaaact
    10141 ttggacatca atgccagagt cttttgacat tgcttccata acgtcacgag tttggtaaca
    10201 tagtgattct atagttgcac ggataaagtg ctctttttca gttccacgtg ataaaccgaa
    10261 aatcgcacca cgtgcttcag aatcccaata tggtgttcct aaacctacaa aagctggaac
    10321 aacataaaca ccctcagtag agtcaactcg tgtcgcataa ctttctgatt gtggtgctga
    10381 attaatcatt cttaatccat cacgtaacca ttggattgct gaacccgaaa caaagatgga
    10441 accttcaagc gcataattta cttttccatc aataccataa gcaattgttg ttaataaacc
    10501 actttcagat ttaaccgctt tgtcacctgt attcattaac atgaagccac cagttccata
    10561 tgtgtttttc acgtcaccac gttcgaagca agcttgtcca aataatgctg cttgttgatc
    10621 accagctaca ccagcgattg gtacttcttg accatagaag tggtaatcaa ttgtcttacc
    10681 atatacttca cttgaaggtt taacttctgg caacatattt ttaggtactg taagtagttc
    10741 taataactca tcgtcccatt ctaaatcatg gatattaaac attaatgtac gactcgcatt
    10801 tgaataatca gtaatatgcg cagcttttcc tgataatttc catactaacc aagtatcaat
    10861 cgttccaaat aatagatcgc cattttctgc tttttctcgt gcaccttcaa cattgtctag
    10921 aatccattta acttttgtac ctgcaaaata cggatctaaa agtaatcctg tcttatctct
    10981 aaatgtttgt tcatatcctt gttgttttaa ttctgaacaa attgattgtg tttgacgtga
    11041 ttgccaaaca attgcgtgat aaattgggcg gccagtatgt ttgtcccaaa ccaccgttgt
    11101 ttcgcgttgg tttgtaatac cgatacctgc aatttgatca gctctaacat cattttcatt
    11161 aattacttcc gtcattacag ctaacacaga tgtccaaatt tcatttgcat catgttcaac
    11221 ccaacctgat tgtggaaaat attgcttaaa ctcacgttgt gctacccctg caatttcccc
    11281 tttttgattg aataaaatcg ctcttgagct tgttgttcct tggtctatag ataaaatata
    11341 tttttccatt agtagtatct cctttattaa aaaataatcg ctaacccatt gtacaaaatc
    11401 tgttaaattt tggcgaaaaa atagcataat ccatgaatca aacatgttaa attatgctat
    11461 ttacgtttct ttttatttta gtaaattgat tcgatatcac ctttttttga tgatttattt
    11521 aaaatgtaac ctaaaatcaa agtaataata actacaacaa ttgcaattgc acaaccaata
    11581 ttaaatgtat gtttataaaa tacagcgtat accactgcac ctaataaacc accggcaatt
    11641 ggtcctaaga taggaacgat tgcatatgac caatttgaac cacctttacc agctattggt
    11701 aaaatcgcat gtgcaattct cggacctaaa tcacgtgctg ggttgattgc ataaccagta
    11761 gcaccgccta aacttaatcc gattgcaaca attaatgctc cgacaattaa aggatttaaa
    11821 ccatcggcaa ttttgtttac accgataaat aaaataccta aagttaatgc cattgttccg
    11881 ataatctcac ttaaaaagtt ggcaaagtaa ttcttaatag ccggtgctgt agagaaaaca
    11941 cctaatttcg cgccagcttc ttctgtcgct ttccaatgtg gcaagtacat taaccataca
    12001 attgttgctc cgacaattgc acctaacatt tgagcaacaa tataaccagg aactaatgac
    12061 caatcaaaac ttccgtctaa tgcaagagct aaagacaccg ctgggtttaa atgtgcacct
    12121 gagaattgac cgacagcaaa cacacccatt gtaaccgcta atccccatcc agctgtgatg
    12181 acaatccaat cagcaccatt cgcagcactt ctctttaaat tgacattggc acaaacgcca
    12241 ccaccaaaaa ggattaagat tgcagttcct aggaattctg ctaaatatac attcatatta
    12301 aatgtcctcc caaaaagaca aagagattcc tacactagca gtttggctcg tagaaatctc
    12361 ccgtctctat tctatgtatt aacttttatt atatttgtta tgatactatc gatatgaaag
    12421 cgttgtcaat ggtttttgta aaaattttgt caaatttaat ttttcaagtc tttataaaag
    12481 tagtttaatt tgaaaactag aaatatcaat ccgcactaat ttatttatga cgccattatg
    12541 tttaattgac attcttcagc catatttttt atgactagtt gcatttttta ctaataaaca
    12601 cctctaaaac tt
//
LOCUS       DAMZKC010000073        12594 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.073, whole genome shotgun sequence.
ACCESSION   DAMZKC010000073 DAMZKC010000000
VERSION     DAMZKC010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12594
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.073"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(478..3525)
                     /gene="fnbA"
                     /locus_tag="POB55_001485"
     CDS             complement(478..3525)
                     /gene="fnbA"
                     /locus_tag="POB55_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein FnbA"
                     /protein_id="HDI6500451.1"
                     /translation="MKNNLRYGIRKHKLGAASVFLGTMIVVGMGQDKEAAASEQKTTT
                     VEENGNSATENKVNETQTTTTNVNTIDETQSYSATATEQPSNATQVTTEEAPKAVQAP
                     QTAQPANLETVKEEVVKEEAKPQVKETTQSQDNSGDQRQVDLTPKKATQNQVAETQVE
                     VAQPRTASESKPRVTRSADVVEAKEASDEKVETGTDVTSKVTVESGSIEAPQGNKVEP
                     HAGQRVVLKYKLKFADGLKRGDYFDFTLSNNVNTYGVSTARKVPEIKNGSVVMATGEI
                     LGNGNIRYTFTNEIEHKVEVTANLEINLFIDPKTVQSNGEQKITSKLNGEETEKTIPV
                     VYNPGVSNSYTNVNGSIETFNKESNKFTHIAYIKPMNGNQSNTVSVTGTLTEGSNLAG
                     GQPTVKVYEYLGKKDELPQSVYANTSDTNKFKDVTKEMNGKLSVQDNGSYSLNLDKLD
                     KTYVIHYTGEYLQGSDQVNFRTELYGYPERAYKSYYVYGGYRLTWDNGLVLYSNKADG
                     NGKNGQIIQNNDFEYKEDTAKGTMSGQYDAKQIIETEENQDNTPLDIDYHTAIDGEGG
                     YVDGYIETIEETDSSAIDIDYHTAVDSEAGHVGGYTESSEESNPIDFEESTHENSKHH
                     ADVVEYEEDTNPGGGQVTTESNLVEFDEESTKGIVTGAVSDHTTIEDTKEYTTESNLI
                     ELVDELPEEHGQAQGPIEEITENNHHISHSGLGTENGHGNYGVIEEIEENSHVDIKSE
                     LGYEGGQNSGNQSFEEDTEEDKPKYEQGGNIVDIDFDSVPQIQGQNNGNQSFEEDTEK
                     DKPKYEQGGNIIDIDFDSVPQIHGFNKHTEIIEEDTNKDKPNYQFGGHNSVDFEEDTL
                     PKVSGQNEGQQTIEEDTTPPTPPTPEVPSEPETPTPPTPEVPSEPETPTPPTPEVPSE
                     PETPTPPTPEVPAEPGKPVPPAKEEPKKPSKPVEQGKVVTPVIEINEKVKAVAPTKKA
                     QSKKSELPETGGEESTNKGMLFGGLFSILGLALLRRNKKNNKA"
     gene            complement(3509..3611)
                     /locus_tag="POB55_001486"
                     /pseudo
     CDS             complement(3509..3611)
                     /locus_tag="POB55_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501263.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3994..5352)
                     /locus_tag="POB55_001487"
     CDS             complement(3994..5352)
                     /locus_tag="POB55_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease"
                     /protein_id="HDI6500452.1"
                     /translation="MFNEIWPLISVVLGIVILLVLIIGFKLNTFISLIITSMITALML
                     GIPLTKIMETIEKGMGSTLGHIALIFGLGAILGKLLADGGGATRIADTLIQKFGQKHV
                     QWAMLVAAFIVGIALFFEVGLVLLIPLVFTVAKRANVSVLKLGLPMVTALSVTHGFLP
                     PHPGPVVIAKELKANVGDVLLYGMIIAIPVTLIAGPIFNKVAQKMIPSAYTREGDISA
                     LGAQKEFTDQEMPGFGMSLLTATLPVILMLVSTITQLVTGHDKPTNLFESIIYMIGTA
                     GTAMLIAVLFAIVTMGLMRKRKMNHIMESVTNAIYPIGMMLLIIGGGGTFKQVLIDGG
                     VGNTIAKMFEGTEMSPILLAWIVAAVLRIALGSATVAAISTTGIVLPLLQSSDVNVAL
                     VVLAIGAGSVILSHVNDAGFWMFKEYFGLTVKETFLTWSLLETIISVSGIIFILFISL
                     FV"
     gene            complement(5469..7022)
                     /gene="gntK"
                     /locus_tag="POB55_001488"
     CDS             complement(5469..7022)
                     /gene="gntK"
                     /locus_tag="POB55_001488"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HDI6500453.1"
                     /translation="MKYMIGVDIGTTSTKSVLYDENGTFIMKHQIGYDLHTPNVDVSE
                     ENPDELFDAVLMTIKYVMRESKVNKDDIKFVSFSAQMHSLIAMDQKHQRLTNNITWAD
                     NRAAKYATVINEVHDGNAIYQRTGTPIHPMSPLVKIFWMKHEWQDVFQRTAKFADIKT
                     YIFYHLFDTYIIDYSMASATGMFNLETLDWDVGALELLGISKEMLPELVPTTYVMKGM
                     KERYATLMGLNEDTPFVIGASDGVLSNLGVNSVGKGEVAVTIGTSGAIRTVIDKPRTD
                     YKGRIFCYVLTEDHYVIGGPVNNGGVVLRWLRDELLASEVETAKRLGVDPYDVLTQIA
                     KRVKPGADGLIFHPYLAGERAPLWNANARGSFFGLTLSHKKEHMIRAALEGVLYNLYT
                     VYLALIEVMNETPKMIKATGGFAKSEVWRQMMSDIFDTELVVPESYESSCLGACVLGL
                     KAVGDIEDFSIVSSMVGATNNHMPFEENVAVYQELVSIFINLSRSLTENYEQIADFQR
                     QHMAENKTQ"
     gene            complement(7046..7726)
                     /locus_tag="POB55_001489"
     CDS             complement(7046..7726)
                     /locus_tag="POB55_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDI6500454.1"
                     /translation="MMYGYPEKWLEGMTTGEGIAAELRLGIVNGHIAEGTLLTENQMA
                     KQFNVSRSPIRDAFKLLQQNQLIQLERMGAHVLPFGEQEKKEMYDLRLMLESFAFSRV
                     KNQERLPIVKEMKKQLEMMKVAVKFEDAESFTKHDFEFHETLIKASNHQYLNSFWSHL
                     KPVMMALVLTSMRQRMQQNPQDFERIHHNHQVFIDAVEQYDSQILKEAFHLNFDDVGK
                     DIEGFWLN"
     gene            complement(7880..8644)
                     /locus_tag="POB55_001490"
     CDS             complement(7880..8644)
                     /locus_tag="POB55_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDI6500455.1"
                     /translation="MSNYSTGELAKLCNVTTRTIQYYDRKGILKPQGFTEGKRRVYTE
                     QQRQTLELILLLKDLGCALSDIDMLLKGEGTLKTLNTLLTMKQQEINQQVKQQQAVLN
                     KIKNVQYYVNKASTSPITHLKDIEHVMSKSAEMKSIRRNIWISAGIIGIIQYSSIISA
                     ILMKNKWPFLIALPFMIGYGIGVTFYYQQKVAYLCPNCQHIFSPSLWQVIKAKHTATT
                     RRFECPNCHETHYCIEVPKAHMSTEQLEISHIQHNN"
     gene            complement(8809..9501)
                     /locus_tag="POB55_001491"
     CDS             complement(8809..9501)
                     /locus_tag="POB55_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HDI6500456.1"
                     /translation="MYVDRKPSLYLEDLRHDFKNSLSKFENGDEAFDTLLGFVELDHI
                     YSSALKEISTKLSILDDNFNHIYKHNPIHHMERRVKEMRSLIEKLNRKGLQISAETAK
                     EHILDIAGIRVVCNYLDDIYLIEEMLLKQEDVQLIKRKDYIQHPKENGYRSLHIVVSI
                     PVFLAERVEVLPVEIQIRTIGMDMWASLEHKIRYKNNAETEKYRDLLKECATEITEVE
                     DKLQQIHSEITE"
     gene            complement(9732..10148)
                     /locus_tag="POB55_001492"
     CDS             complement(9732..10148)
                     /locus_tag="POB55_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HDI6500457.1"
                     /translation="MPFTMKGYPEKWKDFEVIKRQKAIEIANAMLKEGYLEKDVVPIA
                     AKKAKDWYRALSKEEIKALEEENFVQFHTATMEKTEVDSEYATTDNEEHIQEQSNQDK
                     SYFDSESIGNQSGGVRQREPLMDGPHPKVVESFKDV"
     gene            complement(10287..12110)
                     /locus_tag="POB55_001493"
     CDS             complement(10287..12110)
                     /locus_tag="POB55_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDI6500458.1"
                     /translation="MIIPAIIGYLCGNFQFGLLVATGTLAHIYVFKGPSRSKLRTVII
                     CNLAFAICMMLGTLTAKTPLVFGMTLLIVTVIPFYIFTALKIAGPSSTFFIVTFSLPI
                     NLPIAPEEALYRGFAILVGGILATMMVLITIVFSKNKAEEQAIQNDFKLISKLLHTYN
                     DKSAFLQVAKTAVDNFKASDKLLITSTSSNDKLSRRFQKLLLLHTSAQGIYSELLELN
                     AKQIRPLPDELIEMMDHIIAQLDNSEENVRYWRKEVTVTEEFQNLFNHIMKIDEMVHA
                     NEARIAYEADMRKPLYSKRIYQNLTLDSIVFRNTLRYTAIMMIAIFIALMFDFEKAYW
                     IPLSAHTILLGTSTIHAIERGMARGLGTILGVLVLSVILLFSIPTPVAVILMGIAALF
                     TEALVGANYAIAVVFITIQVILMNGLASQNLTINIAFPRVIDVAMGIVIAIIGLFVLG
                     QRTASALLPNVMAEVVRKEATLFHYLFSENQYKDDVYQKNTAMNLSVKLNNMTQVYNA
                     ANGELFSDKTLIQNYYPSLFALEEISFMLNRAMANEDRLTINEQLMGEYLATFENIAK
                     HLELNTELEIKILPDLPQYNYIQSAMMNIQHNSFCERDKNV"
BASE COUNT         3685 a         2455 c         1859 g         4595 t
ORIGIN      
        1 caggcttgta ttttttatac ttacttttta aattaaatta aaattcatct aattcaaaac
       61 aatatactaa aacatacata ataatcgcct gtacaatgca tcattaacaa gtcactgaaa
      121 cgcctttcat tgtattaata acgtcactac aatttttata tcgttcggtt tttgtttggt
      181 tttaatgatt gtttatacag aaacagccgt atttcaagac gatattttaa atttaactaa
      241 atttgcatct agttaataat tgcatttatc taatttgtct tattgattca atctaatttg
      301 tacttacaaa ctagttttaa attctaactt tatctctcag ttcgttatca atcatcagac
      361 ataaaccaat gaagcaatca gaaaacactc taattttcta ttagaaattt gatttaatct
      421 aaaaaaacag gtttacttca tataatttat gaaataaacc cgtcaatttt tgtttaatta
      481 tgctttgtta ttctttttat ttctgcgtaa taatgctaaa cctagaatgc tgaataatcc
      541 gccgaacaac atacctttgt ttgttgattc ttctccacct gtttcaggta gttcagattt
      601 cttagattgt gcttttttag ttggtgccac tgctttaacc ttttcattga tttcaataac
      661 aggtgttact actttacctt gttccactgg tttagaaggc tttttaggtt cttcttttgc
      721 gggtggtact ggtttaccag gttcagctgg tacctctggt gttggcggtg ttggtgtttc
      781 tggctcactc ggcacttctg gtgtcggtgg tgttggtgtt tccggctcac ttggtacttc
      841 cggagtcggt ggtgttggtg tttccggctc acttggtact tctggtgtcg gtggtgttgg
      901 cggcgttgta tcttcttcaa tcgtttgttg accttcattt tgaccgctta cttttggaag
      961 tgtatcttct tcaaaatcaa cactgttgtg tccaccgaat tgataatttg gtttatcttt
     1021 gtttgtatct tcttcaataa tttcagtgtg cttattgaat ccatgaattt gtggcacact
     1081 gtcgaagtcg atatcaatga tattaccacc ttgttcatac ttaggtttgt ctttctctgt
     1141 atcttcttcg aatgactggt taccattatt ttgaccttgg atttgaggta cactgtcgaa
     1201 atcgatatct acgatattgc caccttgttc atatttaggt ttgtcttctt ctgtgtcttc
     1261 ctcgaatgac tggttaccgc tattttggcc accttcataa cctaattcac tcttaatatc
     1321 aacatgacta ttttcttcga tttcttcaat cacgccataa ttaccgtgac cattttcagt
     1381 tcctaaacca gaatgagaaa tatgatgatt gttttcagtg atttcctcga ttggaccttg
     1441 agcttgacca tgttcttctg gtagttcatc cactagttca atcagattac tttcagtcgt
     1501 atattctttc gtatcttcaa ttgttgtatg atcgctcact gcgccagtta caataccttt
     1561 tgtagactct tcgtcaaatt caactaagtt agactcagta gtaacctgac caccacctgg
     1621 atttgtatcc tcttcatatt caacaacatc agcgtgatgt tttgaatttt catgtgtcga
     1681 ttcttcaaag tcaattggat ttgattcctc agaggactca gtgtatcctc caacgtgacc
     1741 cgcttcacta tccacagcag tatggtaatc gatatcaata gctgatgaat ccgtttcttc
     1801 tattgtttca atatacccat caacataacc accctcacca tctatagctg tgtggtaatc
     1861 aatgtcaagc ggtgtattgt cttgattttc ttctgtttca ataatttgct tggcatcgta
     1921 ctgcccgctc atagttcctt ttgcagtatc ttctttatat tcaaaatcat tattttgaat
     1981 aatttgtcca tttttaccat tgccgtcagc tttattgcta tataaaacta aaccattatc
     2041 ccaagttaaa cgatatcccc cataaacata gtaagattta tatgctcgtt ctggataccc
     2101 atataattca gttctaaaat taacctgatc tgacccttgc aaatattcac ctgtataatg
     2161 aatgacatac gttttatcca acttatctaa attcaatgag taactaccat tgtcttgcac
     2221 actcaatttt ccattcattt cctttgttac atctttgaat ttgttagtat ctgatgtatt
     2281 tgcataaaca ctttgtggca attcatcttt tttccctaga tattcatata ctttaacagt
     2341 aggttgtcca ccagctaaat tactaccttc agtcaacgtc cctgttactg atacagtgtt
     2401 tgactggttt ccattcattg gcttaatata agctatatgt gtaaatttat tagattcttt
     2461 attaaatgtt tcaattgatc catttacatt tgtataacta ttgctaacac ctggattata
     2521 aacaactggt attgtttttt ctgtttcttc accatttaat ttagaagtaa tcttttgttc
     2581 tccattgctt tgtacagttt tagggtcaat aaataagttg atttctaaat tagctgttac
     2641 ctctaccttg tgttcaattt cgttagtaaa tgtatatctt atgttaccat tccctaagat
     2701 ctcacctgta gccattacaa ctgagccatt tttaatctct ggtacctttc tagctgttga
     2761 aaccccataa gtatttacat tatttgataa tgtaaaatca aaataatctc ctctttttaa
     2821 tccatctgcg aatttcaatt tgtatttcaa tacgacacgt tgaccagcat gtggctctac
     2881 tttatttcct tgaggtgcct caatagaacc actttctact gtaactttac ttgtcacatc
     2941 tgtacccgtt tccactttct cgtcactagc ttccttagct tcaactacgt ctgctgatct
     3001 tgtcacacgt ggtttacttt ctgatgccgt tcttggctgt gccacttcaa cttgtgtttc
     3061 tgcgacttga ttttgtgtgg cctttttagg tgttaaatct acttgtcttt gatctccgct
     3121 attgtcttga gattgtgttg tttccttaac ttgaggtttc gcttcttcct taactacctc
     3181 ttctttaact gtttctaaat ttgctggttg tgcagtttgt ggtgcttgta ctgcttttgg
     3241 tgcttcttca gttgttactt gtgttgcgtt tgacggttgt tctgttgctg ttgcgctgta
     3301 tgattgtgtt tcatctatag tattaacgtt agttgtagtt gtttgtgttt cgtttacttt
     3361 attttcagta gctgaattcc cattttcttc tactgtagtt gtcttttgtt ctgatgctgc
     3421 agcttcttta tcttgtccca tcccaacaac gatcattgtt cctaagaata ctgatgctgc
     3481 tcccaattta tgttttctaa tgccgtacct aagattgttt ttcactataa tatctccctt
     3541 taaatgcaaa attcattaat ttttttaaac ttaataaatg caagtctata ttgttcggtt
     3601 tttaaaagca atacaaaaaa gcgagttaat gaaaagttaa gattgtcatt aattttatgt
     3661 taattgataa ttttattatt tgaaatatac ctataaattg tattcaagtc atcataaacc
     3721 cttgtcacac aaggcttgta ttttttatac ttatttttca aattaaattc atcattatct
     3781 aatttaaaac aatatactaa gtgtttcata attatcgcct gtacaatacg cacaaaaaca
     3841 tgtcttgaaa cgcctttcat tacactaaaa tccccaatat actttttata tcgttcggat
     3901 tatgactatt tcagacgatt ttctgcataa aaataaacgt gtttcaaggc aatatattgc
     3961 aattacctta aaacacgttt acttaatatt tagttaaaca aataagctga tgaatagaat
     4021 gaagatgata cctgaaacgg aaataatcgt ttctaataat gaccatgtta agaatgtttc
     4081 ttttacagtt aaaccaaaat attctttaaa catccaaaat cctgcgtcat ttacatgaga
     4141 caaaatcaca ctacctgcac ctatcgcaag tacaactaat gcaacattta catctgatga
     4201 ttgtaataat ggtaagacaa tacctgtagt tgaaatcgca gctactgtag ccgaacctaa
     4261 tgcgatacgt agcacagctg caacaatcca tgctagtaaa attggagaca tctctgtacc
     4321 ttcaaacatt ttagcaattg tatttccgac accaccgtca attaatactt gtttaaatgt
     4381 accgccaccg ccaataatca ataacatcat tccgattgga taaatcgcat tcgtcactga
     4441 ttccataata tgattcatct tacgttttct cattaatccc atcgtaacga ttgcaaataa
     4501 tactgctatt agcatggctg tccctgctgt tcctatcata taaatgatag attcaaatag
     4561 atttgtaggt ttgtcgtgcc cagttacaag ttgcgttatc gtagacacta acattaatat
     4621 gactggtaat gttgcagtta ataaactcat accaaatcct ggcatctctt gatccgtaaa
     4681 ttctttttgt gcacctaacg ctgaaatatc gccttctcgt gtatacgcag acggaatcat
     4741 tttttgtgca actttgttaa atataggtcc tgcaatgagt gtaactggaa tggcaataat
     4801 cataccgtac agtaatacat ctccaacatt tgcctttaat tcttttgcga tgactaccgg
     4861 tcctggatgt ggtggtaaaa agccatgtgt cacagataaa gctgttacca taggtagtcc
     4921 tagttttaac actgaaacat ttgcgcgttt tgctactgta aatactaatg gaatcagtaa
     4981 gactaaacct acttcaaaga acaatgcaat accgacgata aatgctgcaa caagcattgc
     5041 ccattgtaca tgtttttgac caaatttttg aatcaacgtg tctgcaattc gagttgcacc
     5101 accaccatca gcaagtaatt tcccaagtat ggcacctaaa ccgaatatta gtgcaatgtg
     5161 gccgagcgta ctgcccatcc ctttctcaat cgtctccata attttagtca atggtatacc
     5221 tagcattaac gctgtaatca tcgatgtgat aattaatgaa ataaatgtat ttaatttaaa
     5281 cccaataatt aatactaata aaataacgat acctaaaaca acactgatta acggccatat
     5341 ttcgttaaac atgacattcc cctctttctc ttttcaatag aatgtaacac cgtcgtcgcg
     5401 cacgtattcc acataatata tagtagtcat ggcacaacaa ctttatctaa gatgcttaag
     5461 tgacgtattt attgtgtttt attttcagcc atatgttggc gttgaaaatc tgcaatttgt
     5521 tcataattct ctgttaaaga acgacttaaa ttgataaaaa tggatacgag ctcttggtaa
     5581 acagcgacat tttcttcaaa cggcatatga ttatttgtag caccgaccat cgatgaaacg
     5641 attgaaaaat cttcaatgtc acctacagct ttaagtccaa gcacgcaggc acctaagcat
     5701 gaactttcat agctttcagg aaccactaac tctgtgtcaa atatatctga catcatttga
     5761 cgccatactt cacttttcgc aaaaccacct gttgctttta tcatcttagg cgtttcattc
     5821 attacttcaa tgagtgcaag gtagacggta tacaaattgt aaagaacacc ttctaatgca
     5881 gcgcgaatca tatgttcttt tttatgagat aaagttaaac cgaagaatga acctcttgca
     5941 tttgcgttcc aaagcggcgc acgttctcca gctaaatagg gatggaatat taaaccatct
     6001 gcacctggtt taacacgctt tgcaatttga gttaagacat cataaggatc aacaccgaga
     6061 cgtttcgcag tttcgacttc actcgctagc aactcgtcgc gcaaccatct caatacgaca
     6121 ccaccattat ttacaggacc tccgatgacg tagtggtcct ctgttaagac ataacaaaat
     6181 attctacctt tgtaatcagt acgcggttta tctatcacag tacgaatcgc cccagatgta
     6241 ccgattgtga cagcaacttc tcctttacca acgctattga cacccaaatt agaaaggacc
     6301 ccatcactcg caccaataac aaacggtgta tcttcattaa gccccattaa tgttgcataa
     6361 cgttctttca tgcctttcat tacgtacgtt gttggcacta attccggcaa catttccttg
     6421 gaaataccaa gcaattctaa tgccccaaca tcccaatcta atgtttctaa attaaacatc
     6481 cctgttgcgg aagccattga ataatcaatg atatatgtat caaataaatg atagaaaatg
     6541 tatgttttaa tatctgcaaa cttagcagta cgttgaaata catcttgcca ttcatgtttc
     6601 atccaaaaaa ttttcactaa aggcgacata ggatgaatag gcgtacctgt tcgctgataa
     6661 atcgcattgc catcatgcac ttcatttatt actgttgcat attttgcagc gcggttatct
     6721 gcccaagtaa tattatttgt taatctttga tgtttctgat ccatcgcaat caagctatgc
     6781 atttgcgcac taaatgacac aaatttaata tcatctttat taactttgga ttctctcata
     6841 acatatttaa tagtcattaa taccgcatca aataattcat ctgggttttc ttctgagaca
     6901 tcaacgtttg gtgtgtgtaa atcatagccg atttgatgtt tcatgatgaa tgttccattt
     6961 tcatcatata agactgactt ggtactcgtc gttccaatgt cgacaccaat catatatttc
     7021 atgacaaatc cttctttctt tcattttaat tcaaccaaaa tccttcaata tctttaccaa
     7081 catcgtcgaa atttaagtga aacgcttctt tcaaaatttg actgtcgtat tgttccactg
     7141 catcaataaa cacttgatga ttatgatgta tgcgttcaaa atcttgcggg ttctgttgca
     7201 tacgttgtcg catggatgtt aaaacgagtg ccatcattac tggttttaaa tgactccaaa
     7261 atgagtttaa atattgatga ttagatgctt tgattaatgt ttcatgaaat tcaaagtcat
     7321 gcttcgtaaa tgattctgca tcctcaaatt ttactgccac tttcatcatt tcaagttgtt
     7381 tcttcatttc ttttacgata ggtagtcgct cttgattttt aactcttgaa aatgcaaaag
     7441 actctagcat cagtcgcaaa tcgtacattt ctttcttttc ttgttcccca aacggcaaca
     7501 catgtgcacc cattctttct aattggatga gttgattttg ttgcaataat ttaaatgcat
     7561 ctcgaattgg cgaacgactc acattaaatt gctttgccat ttgattttca gtgagtaacg
     7621 taccttcagc tatgtgacca ttcacaatac ctaagcgtaa ttctgccgcg ataccttctc
     7681 cagttgtcat accttccaac catttctctg gatatccata catcatcaaa gccactcctt
     7741 cattacacga catacttgta tacaagtatg ttaatatagt tcttaatcgt ttgcaagcgc
     7801 tttctttacg cgtactaaaa tagtgaccat cccttttcaa tgtaaattta aagggaatgg
     7861 tcactatcac acgaatgatt taattgttat gttgtatgtg ggatatttct aattgttctg
     7921 tactcatatg cgctttaggt acttcaatgc aataatgcgt ttcatgacag tttggacatt
     7981 cgaatcgacg tgttgtagca gtatgtttcg ctttgataac ttgccataaa gatggtgaga
     8041 atatatgctg gcagttagga cataaatagg caaccttttg ttggtaataa aaagtaacac
     8101 caatgccgta tccaatcata aacggcaacg caattagaaa cggccattta tttttcatca
     8161 aaattgcact tataatgcta gaatattgaa ttattcctat aatacccgca ctaatccaaa
     8221 tgttacgacg aatacttttc atttcagctg atttactcat gacatgctct atgtctttta
     8281 aatgtgtgat tggagacgtc gacgctttat ttacgtaata ttgaacattt ttaattttgt
     8341 ttaataccgc ttgttgctgt ttgacttgtt gattaatttc ttgttgtttc atagttagta
     8401 aagtattgag tgtcttcaaa gtaccttcac cttttagcaa catatctata tcgcttaacg
     8461 cacaacctaa atctttaagc aataagatta actctaatgt ttgtcgctgt tgttctgtat
     8521 acacacgacg ctttccttct gtaaatcctt gtggtttcaa aatacctttg cgatcataat
     8581 attgaatcgt tcgcgttgtc acattgcata atttcgcgag ttctccagtc gaatagttag
     8641 acatagattc cacctcctac aatcaccata gttgatgacc cgacgtcacg agcaagtact
     8701 ttttctacat tttaaagaaa tttattatac taggcgtctt atttttgtga tttcgtacca
     8761 tgttgattta caaaatcact caaattaagc aacactacta ctaaacgtct actctgttat
     8821 ttcagaatga atttgttgta atttatcttc aacttcagta atctctgtcg cacattcttt
     8881 cagtaaatct cgatactttt ccgtctctgc attgttttta taacgtattt tatgttctaa
     8941 acttgcccac atatccatac ctatcgttct aatttgaatt tcaacaggca atacctcaac
     9001 gcgttctgct aaaaagactg gaatggatac aacgatatgt aaactgcggt aaccattttc
     9061 tttagggtgc tgaatataat ctttacgttt tatcaattgt acgtcttctt gtttaagcaa
     9121 catctcttca atcaaataaa tatcatctaa gtaattacat actacgcgaa ttccggcaat
     9181 atccagtata tgttctttgg cagtttctgc gctaatctgt aatcctttac gattaagctt
     9241 ttctattaaa ctgcgcattt ctttcacacg tcgctccata tgatgtatag ggttgtgttt
     9301 ataaatgtga ttgaaattat cgtctaaaat actcagttta gtgcttattt cctttagtgc
     9361 tgacgaataa atatgatcta actctacgaa acctaataac gtatcaaatg cttcatcacc
     9421 attttcaaat ttacttaaac tatttttaaa atcatgtcgc aaatcctcta aatatagtga
     9481 tggttttcga tctacataca tttttatact aacctccgat atataatcat ctttattgta
     9541 cctaacattt tattaagata atactaatac actatcgaac tttgggctga tactgaaaca
     9601 gcacaaagaa actcaaacaa ttcgaattat gtatcaaaac cttcaattat aggaagtaat
     9661 tttaaaatga tttaacgcaa aaaaacacct gttaccgtta tataggtaca agtgtttaat
     9721 tgatagagat gttatacgtc tttaaatgat tccacaactt ttggatgtgg accatccata
     9781 agcggttctc tttgtcgaac gccaccactt tggttgccaa ttgattcgct atcaaagtac
     9841 gatttatctt gatttgattg ttcttgaata tgttcttcat tatcagttgt tgcatattca
     9901 ctatccacct ctgttttttc cattgttgct gtatggaatt gtacgaagtt ctcttcttct
     9961 aatgctttga tttcttcttt cgataacgct cgataccaat ctttcgcttt tttcgccgca
    10021 ataggaacaa catctttttc aagatatcct tcttttaaca ttgcattcgc aatttctatc
    10081 gctttttgcc gtttaattac ttcaaaatct ttccattttt cggggtaacc tttcattgta
    10141 aagggcattt ccttaacctc caatatgtta taattcatgt tatataccac cataattata
    10201 aaatgaaaca tattaaacac aattttttag actcccgtca taataacttc agacaaacgt
    10261 tagtctgacc tgataaatat tttattttaa acgttcttgt ctctctcaca aaaactattg
    10321 tgttgaatat tcatcattgc tgattgaata taattatact gtggtaaatc tggtaatatc
    10381 tttatttcta attctgtatt taattctaaa tgcttagcga tgttttcaaa tgttgctaaa
    10441 tactcgccca ttaattgttc atttattgta agtctatctt cattagccat ggctctattt
    10501 aacataaagc taatttcttc taatgcgaat aagctaggat aataattttg aatcaatgtc
    10561 ttatcactaa ataattcacc attagctgca ttataaactt gcgtcatgtt atttaatttc
    10621 acagataggt tcatagccgt gtttttctga tacacatcat ctttatattg gttttcagaa
    10681 aacaaataat ggaagagcgt tgcttcttta cgaacaactt cagccattac attaggaagc
    10741 aatgcggatg cggtacgttg tccaaggaca aataaaccta tgattgcaat cacaataccc
    10801 attgcaacgt caataactct tggaaacgca atgttaattg ttaaattctg tgatgctaat
    10861 ccattcatta aaataacttg tattgtaata aaaactactg caatcgcata atttgctccc
    10921 accaatgctt cagtaaacaa tgctgcaatg cccattaaaa ttactgcaac aggtgttggt
    10981 attgaaaaca acaatatgac tgaaagtact aacacaccta aaatagtacc taaacctcgt
    11041 gccatacctc tctcgattgc atgtatagtt gatgttccta gtaatattgt atgtgcagat
    11101 aacggtatcc agtatgcttt ttcaaaatca aacattaacg caataaatat cgctatcatc
    11161 ataatcgctg tatatctcaa tgtatttcta aaaacaatag agtctaatgt taaattttga
    11221 taaatgcgtt tactatataa aggttttcgc atgtctgctt catacgcaat acgcgcttca
    11281 tttgcatgca ccatttcatc aattttcatt atatggttga ataaattttg aaattcctct
    11341 gttactgtca cttcttttcg ccaatatctt acattttcct cactattatc tagttgtgca
    11401 atgatatgat ccatcatttc aattaactca tctggcaatg gtcgaatttg tttagcgttc
    11461 aactctaaca gttcagaata aatcccttgg gcagatgtgt gtaataataa taatttttgg
    11521 aaacgtctac ttaatttatc gttacttgaa gtagaagtga ttaataattt atcagatgct
    11581 ttaaaattat ccaccgctgt ttttgctact tgtaaaaatg cagatttatc attataagtg
    11641 tgtaacaact tagatatgag tttaaaatca ttttgaattg cttgttcttc agctttgttt
    11701 ttagaaaata cgatcgtgat taacaccatc atagtggcaa gtataccgcc tactaaaatc
    11761 gcaaagcctc tatataatgc ttcttcggga gctataggta agttaatggg tagactgaat
    11821 gtcacaatga agaatgtcga tgacggtcca gctattttta aggcagtaaa tatataaaat
    11881 ggtataaccg taacaattaa taatgtcatt ccaaaaacga gtggcgtttt ggctgttagc
    11941 gtaccaagca tcatacatat tgcaaacgct aaattacaaa ttattacagt tcgcagctta
    12001 gatcgcgacg gacctttaaa aacataaata tgggctagtg ttccggttgc aactaataat
    12061 ccaaattgga aattaccaca taagtaaccg attattgcgg gaataatcat taacagtccc
    12121 tgtcttacac ctttataaaa gtctaagtta agtcgattga tttttatgag tgatgtcaaa
    12181 aagatgtcat ttcgacttct cctttccttg tcaaaatatt tccataatct ttttattagc
    12241 atagttaaaa tgaatttgtt gctataaatt ttaattacgc aacatatgtc attataacac
    12301 cttatactca attatctaaa ttcaaaaact tttaaagtcc aattaaaatt attttctaat
    12361 gttatttaac atcgttgaac ctttggtcgc ccacctttgc gctagttaag atttatttgt
    12421 gacatgttga aattatttta gtgaatacga tatttagggt tagaaaaaca ttcacataga
    12481 cttaaggttt atttacttca tattattcct agggttcatg atccaaacta tagtgaattt
    12541 aaaaaagttt ataaaacata tttgcctcat aagcttgaat agttcctata ttcg
//
LOCUS       DAMZKC010000074        12535 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.074, whole genome shotgun sequence.
ACCESSION   DAMZKC010000074 DAMZKC010000000
VERSION     DAMZKC010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12535
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.074"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(85..1539)
                     /locus_tag="POB55_001494"
     CDS             complement(85..1539)
                     /locus_tag="POB55_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDI6500459.1"
                     /translation="MEDNKMNRSLNSRHISMIAIGGAIGTGLFVATGNIISQAGPGGA
                     ILAYLVIGVMLYFLMSSIGELATFYPVSGSFSSYSTRFIDSSLGFTMGWLYWALWSLV
                     TSVDVIVASNVLYFWDTFKFFHPITWSLIFITILLLLNIFSVKSFGETEFWLSLIKVL
                     TIIVFVIFGFLMIFGILGGHTYGFENYTKGQAPFVGGISGFLGVLLVAGFSVGGTEVV
                     AVTAGESDDPKKSMPKAIKQVFWRILLFYVLSIAVIGAIIPYTDPSLLRASSSISQSP
                     FTIVFDRVGIAFAASVINAVILTSLLSAANSGVYTTGRMLYSLSSDKKAPQFLSKLNK
                     TTKLPLRALLTTYAVVVIVIIYANFNSNAVFNLLEIIGSMIIVVWGSSIWSQIRLRQA
                     IKKQGQDPNKVLPYKAPFYPLGPIIVITTLLFLLFGGSVEYILKDQWLNAFKNFLPLI
                     ILALIYFIHKIIHKTKFVKLETINLKPHDYDNQK"
     gene            1743..3260
                     /locus_tag="POB55_001495"
     CDS             1743..3260
                     /locus_tag="POB55_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HDI6500460.1"
                     /translation="MSQQDKKLTGVFGHPVSDRENSMTAGPRGPLLMQDIYFLEQMSQ
                     FDREVIPERRMHAKGSGAFGTFTVTKDITKYTNAKIFSEIGKQTEMFARFSTVAGERG
                     AADAERDIRGFALKFYTEEGNWDLVGNNTPVFFFRDPKLFVSLNRAVKRDPRTNMRDA
                     QNNWDFWTGLPEALHQVTILMSDRGIPKDLRHMHGFGSHTYSMYNDSGERVWVKFHFR
                     TQQGIENLTDEEAAEIIATDRDSSQRDLFEAIEKGDYPKWTMYIQVMTEEQAKNHKDN
                     PFDLTKVWYHDEYPLIEVGEFELNRNPDNYFMDVEQAAFAPTNIIPGLDFSPDKMLQG
                     RLFSYGDAQRYRLGVNHWQIPVNQPKGVGIENICPFSRDGQMRVVDNNQGGGTHYYPN
                     NHGKFDSQPEYKKPPFPTDGYGYEYNQRQDDDNYFEQPGKLFRLQSEDAKERIFTNTA
                     NAMEGVTDDVKRRHIRHCYKADPEYGKGVAKALGIDINSIDLETENDETYENFEK"
     gene            3351..3500
                     /gene="rpmG"
                     /locus_tag="POB55_001496"
     CDS             3351..3500
                     /gene="rpmG"
                     /locus_tag="POB55_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDI6500461.1"
                     /translation="MRVNVTLACTECGDRNYITTKNKRNNPERIEMKKYCPRLNKYTL
                     HRETK"
     gene            3954..4223
                     /gene="rpsN"
                     /locus_tag="POB55_001497"
     CDS             3954..4223
                     /gene="rpsN"
                     /locus_tag="POB55_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009384394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDI6500462.1"
                     /translation="MAKKSKIAKERKREELVNKYYELRKELKAKGDYEALRKLPRDSS
                     PTRLTRRCKVTGRPRGVLRKFEMSRIAFREHAHKGQIPGVKKSSW"
     gene            4380..5357
                     /gene="guaC"
                     /locus_tag="POB55_001498"
     CDS             4380..5357
                     /gene="guaC"
                     /locus_tag="POB55_001498"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HDI6500463.1"
                     /translation="MKIFDYEDIQLIPNKCIVESRSECDTTIQFGPKKFKLPVVPANM
                     QTVMNEKLAKWFAENDYFYIMHRFDEEARIPFIKHMQNSGLFASISVGVKKAEFDFIE
                     KLAQEKLIPEYITIDIAHGHSDSVINMIKHIKNHIPDSFVIAGNVGTPEGVRELENAG
                     ADATKVGIGPGRVCITKIKTGFGTGGWQLAALNICSKAARKPLIADGGIRTHGDIAKS
                     IRFGASMVMIGSLFAAHEESPGETVELDGKQYKEYFGSASEFQKGEHKNVEGKKMFVE
                     HKGSLMDTLKEMQQDLQSSISYAGGKDLKSLRTVDYVIVRNSIFNGDRD"
     gene            5379..6395
                     /locus_tag="POB55_001499"
     CDS             5379..6395
                     /locus_tag="POB55_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001789887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HDI6500464.1"
                     /translation="MKKFIVTVVAFLSITIIAPITEFKPFIHLQNEVRQYIDIHINKE
                     TISAENKLDTPKKQQFAFNNIQMNMSKSDVEKTLNKPKRVTFNEYGTKWYTYYDDDYN
                     NFIMISYMKDKVNALYTNQNIITSKSKIKYNTPKSVVRQRLGEPETEIVKGRVRYEQN
                     NKEYDVFHKNHIYTTVFYDKHRRNNVTAVLQVSDAMENRLKEQYGAPSKSLADSFELQ
                     NFDLVNAARKQHQLFTLKYSKQNSETARKHSKDMANNHYFDHTNLKGQSPFDRLKKDG
                     ITFNSAGENLAYGQVSSIYAHQGLMNSIGHRKNILNDTFKILGVGVDFNDEKQPFWTE
                     NYTG"
     gene            complement(6775..7398)
                     /gene="lexA"
                     /locus_tag="POB55_001500"
     CDS             complement(6775..7398)
                     /gene="lexA"
                     /locus_tag="POB55_001500"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HDI6500465.1"
                     /translation="MRELTKRQSEIYNYIKQVVQTKGYPPSVREIGEAVGLASSSTVH
                     GHLSRLEEKGYIRRDPTKPRAIEIVSDQTNDNINMEETIHVPVIGKVTAGVPITAVEN
                     IEEYFPLPEHLTSTHNSDIFILNVVGDSMIEAGILDGDKVIVRSQTIAENGDIIVAMT
                     EEDEATVKRFYKEKNRYRLQPENSTMEPIYLDNVAVIGKVIGLYREM"
     gene            7541..7774
                     /locus_tag="POB55_001501"
     CDS             7541..7774
                     /locus_tag="POB55_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500466.1"
                     /translation="MFYNKYKNVSTYIIIFLVSSAAFAIFLLSANISAHSEQVYEMTD
                     HQIKNNTINKAYEHKDPTNNSEQRDGKVFALIN"
     gene            7910..8149
                     /locus_tag="POB55_001502"
     CDS             7910..8149
                     /locus_tag="POB55_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HDI6500467.1"
                     /translation="MSNSDLNIERINELAKKKKEVGLTQEEAKEQTALRKAYLESFRK
                     GFKQQIENTKVIDPEGNDVTPEKIKEIQQKRDNKN"
     gene            8270..10258
                     /gene="tkt"
                     /locus_tag="POB55_001503"
     CDS             8270..10258
                     /gene="tkt"
                     /locus_tag="POB55_001503"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001661967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDI6500468.1"
                     /translation="MFNEKDQLAVDTLRALSIDTIEKANSGHPGLPMGAAPMAYTLWT
                     RHLNFNPQSKDYFNRDRFVLSAGHGSALLYSLLHVSGSLELEELKQFRQWGSKTPGHP
                     EYRHTDGVEVTTGPLGQGFAMSVGLALAEDHLAGKFNKEGYNVVDHYTYVLASDGDLM
                     EGISHEAASFAGHNKLSKLVVLYDSNDISLDGELNKAFSENTKARFEAYGWNYLLVKD
                     GNDLEEIDKAITTAKSQEGPTIIEVKTTIGFGSPNKAGTNGVHGAPLGEVERKLTFEN
                     YGLDPEKRFNVSEEVYEIFQNTMLKRANEDESQWNSLLEKYAETYPELAEEFKLAISG
                     KLPKNYKDELPRFELGHNGASRADSGTVIQAISKTVPSFFGGSADLAGSNKSNVNDAT
                     DYSSETPEGKNVWFGVREFAMGAAVNGMAAHGGLHPYGATFFVFSDYLKPALRLSSIM
                     GLNATFIFTHDSIAVGEDGPTHEPIEQLAGLRAIPNMNVIRPADGNETRVAWEVALES
                     ESTPTSLVLTRQNLPVLDVPEDVVEEGVRKGAYTVYGSEETPEFLLLASGSEVSLAVE
                     AAKDLEKQGKSVRVVSMPNWNAFEQQSEEYKESVIPSSVTKRVAIEMASPLGWHKYVG
                     TAGKVIAIDGFGASAPGDLVVEKYGFTKENILNQVMSL"
     gene            10536..10778
                     /locus_tag="POB55_001504"
     CDS             10536..10778
                     /locus_tag="POB55_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HDI6500469.1"
                     /translation="MATWLAIIFIVAALILGLIGGFLLARKYMMDYLKKNPPINEEML
                     RMMMMQMGQKPSQKKINQMMTMMNKNMDQNMKSAKK"
     gene            10765..10863
                     /locus_tag="POB55_001505"
     CDS             10765..10863
                     /locus_tag="POB55_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500470.1"
                     /translation="MRKSKFAIDRGYFPDMEMASFYNQINKNKYVY"
     gene            10957..11424
                     /locus_tag="POB55_001506"
     CDS             10957..11424
                     /locus_tag="POB55_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdC family protein"
                     /protein_id="HDI6500471.1"
                     /translation="MLYLIFSIIVALFMGTIVIVIRMKAQNYPVNEKKIVLPPFFMAT
                     GALMYVVPYFRLTGSEMLEAFIIGLLFSTVLIWTSRFEVKGTEIYMKRSKAFPVILIS
                     LLIIRTVMKIFISNEIDPGELGGMFFLLAFCMIVPWRAAMLYKYKKLKKTLIN"
     gene            11548..>12535
                     /locus_tag="POB55_001507"
     CDS             11548..>12535
                     /locus_tag="POB55_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HDI6500472.1"
                     /translation="MKIIHTADWHLGKILNGKQLLEDQAYILDMFVEKMKEEEPDIIV
                     IAGDLYDTTYPSKDAIMLLEQAIGKLNLELRIPIIIISGNHDGKERLNYGASWFEHNQ
                     LFIRTDFTSINSPIEINGVNFYTLPYATVSEMKHYFEDDTIETHQQGITRCIETIAPE
                     IDEDAVNILISHLTVQGGKTSDSERPLTIGTVESVQKGVFDIFDYVMLGHLHHPFSIE
                     DDKIKYSGSLLQYSFSEAGQAKGYRRVTINDGIINDVFIPLKPLRQLEIISGEYNDVI
                     NEKVHVKNKDNYLHFKLKNMSHITDPMMSLKQIYPNTLALTNETFNYNEENNA"
BASE COUNT         4579 a         1752 c         2258 g         3946 t
ORIGIN      
        1 gatattaaag tatatctgct ttacaccatt aaaaagcggc aaatgctata gataatctaa
       61 agcatctgcc gtatgagaat ttatttattt ttgattgtca taatcgtgtg gttttaaatt
      121 aattgtttct agctttacaa attttgtttt gtgaatgatt ttatgaataa agtaaatcaa
      181 cgctagaatg attaaaggta aaaagttttt aaaagcattt aaccattgat cttttaaaat
      241 atattcaact gagccaccaa atagcaagaa taatagtgta gtgatgacaa tgattggtcc
      301 taatggataa aaaggtgctt tatatggtag gaccttatta gggtcttgac cttgtttttt
      361 aatagcttgt cgcaatcgta tttgtgacca aatgcttgat ccccaaacaa ctataatcat
      421 tgaaccaata atttcaagta aattaaaaac ggcatttgaa ttaaagtttg cataaataat
      481 aacaataaca acgactgcat aagtagttaa taatgctctt aaaggtaact tagttgtctt
      541 gtttaattta cttaaaaatt ggggtgcttt tttgtctgaa cttaaggaat acaacattct
      601 gcctgttgta taaacacctg aatttgcagc ggataataat gaagttaaaa taaccgcgtt
      661 gattactgat gctgcaaagg ctatgcctac tctatcgaat acaattgtaa atgggctttg
      721 acttattgaa ctacttgctc ttaataatga tggatctgtg tacggaataa ttgcaccaat
      781 tactgcaatt gataagacat agaataaaag aatacgccaa aatacttgtt taattgcctt
      841 aggcatagac tttttagggt catctgattc accagcagtt actgctacta cttctgtacc
      901 accaaccgaa aatccggcga ctaataatac gcctaagaaa ccagagatac caccaacaaa
      961 cggtgcttgg ccttttgtat agttttcaaa tccatatgta tgaccaccta agataccgaa
     1021 aatcattaaa aagccaaaaa taacgaatac gataattgtt aacactttaa tcaatgataa
     1081 ccaaaactca gtttctccaa atgattttac agaaaaaatg tttaataata gtaaaattgt
     1141 aataaagatt aagctccaag taatggggtg gaaaaattta aatgtgtccc agaaataaag
     1201 cacatttgac gctactatga catcaacact tgtaactaat gaccacaatg cccaatacaa
     1261 ccatcccatg gtaaagccaa gagatgagtc aataaagcgt gttgaataag agctgaatga
     1321 acctgatact ggataaaatg ttgccaactc tccaattgat gacattaaga aatatagcat
     1381 gacaccaata acaagataag cgagtatagc gcctccagga ccagcttgag aaatgatatt
     1441 accagtagct acaaatagac cagtcccaat tgcaccacct atagcaatca tggaaatgtg
     1501 tcttgagtta agactacggt tcattttatt atcttccata tttagtctcc catctattta
     1561 aatataccca ttattgtaag ctttttaagt gtactattca ataactattt agtactgtaa
     1621 agcgaaaaaa ttaaaatttt ctgatttttt aatcatcttg agcatgttta attgtaattt
     1681 tgatggggtt aaattataat atgtattaaa ttataattat tataaattgt ggagggatga
     1741 ctatgtcaca acaagacaaa aagttaactg gtgtttttgg gcatccagta tcagaccgag
     1801 aaaatagtat gacagcaggg cctaggggac ctcttttaat gcaagatatt tactttttag
     1861 agcaaatgtc tcaatttgat agagaagtaa taccagaacg tcgaatgcat gccaaaggtt
     1921 ctggtgcatt tgggacattt actgtaacta aagatataac aaaatatacg aatgctaaaa
     1981 tattctctga aataggtaag caaaccgaaa tgtttgcccg tttctctact gtagcaggag
     2041 aacgtggtgc tgctgatgcg gagcgtgaca ttcgaggatt tgcgttaaag ttctacactg
     2101 aagaagggaa ctgggattta gtagggaata acacaccagt attcttcttt agagatccaa
     2161 agttatttgt tagtttaaat cgtgcggtga aacgagatcc tagaacaaat atgagagatg
     2221 cacaaaataa ctgggatttc tggacgggtc ttccagaagc attgcaccaa gtaacgatct
     2281 taatgtcaga tagagggatt cctaaagatt tacgtcatat gcatgggttc ggttctcaca
     2341 catactctat gtataatgat tctggtgaac gtgtttgggt taaattccat tttagaacgc
     2401 aacaaggtat tgaaaactta actgatgaag aagctgctga aattatagct acagatcgtg
     2461 attcatctca acgcgattta ttcgaagcca ttgaaaaagg tgattatcca aaatggacaa
     2521 tgtatattca agtaatgact gaggaacaag ctaaaaacca taaagataat ccatttgatt
     2581 taacaaaagt atggtatcac gatgagtatc ctctaattga agttggagag tttgaattaa
     2641 atagaaatcc agataattac tttatggatg ttgaacaagc tgcgtttgca ccaactaata
     2701 ttattccagg attagatttt tctccagaca aaatgctgca agggcgttta ttctcatatg
     2761 gcgatgcgca aagatatcga ttaggagtta atcattggca gattcctgta aaccaaccta
     2821 aaggtgttgg tattgaaaat atttgtcctt ttagtagaga tggtcaaatg cgcgtagttg
     2881 acaataatca aggtggagga acacattatt atccaaataa ccatggtaaa tttgattctc
     2941 aacctgaata taaaaagcca ccattcccaa ctgatggata tggctatgaa tataatcaac
     3001 gtcaagatga tgataattat tttgaacaac caggtaaatt gtttagatta caatcagagg
     3061 acgctaaaga aagaattttt acaaatacag caaatgcaat ggaaggcgta acggatgatg
     3121 ttaaacgacg tcatattcgt cattgttaca aagctgaccc agaatatggt aaaggtgttg
     3181 caaaagcatt aggtattgat ataaattcta ttgatcttga aactgaaaat gatgaaacat
     3241 acgaaaactt tgaaaaataa atttgatatg tagtttctat attgcgtagt tgagcagttt
     3301 atgatatcat aataaatcgt aaagattcct aacaagagag ggtgtttaac gtgcgcgtaa
     3361 acgtaacatt agcatgcaca gaatgtggcg atcgtaacta tatcactact aaaaataaac
     3421 gtaataatcc tgagcgtatt gaaatgaaaa aatattgccc aagattaaac aaatatacgt
     3481 tacatcgtga aactaagtaa ttcttatcat tcaaatacga cgatttgaaa ataaagcggg
     3541 cttacctatt atattgggga gctcgctttt ttatgaaatt tttgtgaaga gtgattaatg
     3601 gattgagttt catcggtaga acaatatatg attgtattag ttgttacttt attaaaattt
     3661 gagaatattt atagaaggaa atagattact gattttataa agtcactttg ttagcgaatg
     3721 cttgaaagag tatttaatat agtagaattt aaaatttcaa agcggaattt aataagtacg
     3781 aagtaattct gggtatgttt tataaatgtt cgataataca ctttaatctt aaatatgatg
     3841 gtttagaaaa tgatttaaca aagaaatgaa actttactgt tgaattatgt gaggattgtg
     3901 ttattatata aatcgtaata attacgattt gataaaaagt gaggtaacta tatatggcta
     3961 agaaatctaa aatagcaaaa gagagaaaaa gagaagagtt agtaaataaa tattacgaat
     4021 tacgtaaaga gttaaaagca aaaggtgatt acgaagcgtt aagaaaatta ccaagagatt
     4081 catcacctac acgtttaact agaagatgta aagtaactgg aagacctaga ggtgtattac
     4141 gtaaatttga aatgtctcgt attgcgttta gagaacatgc gcacaaagga caaattccag
     4201 gtgttaaaaa atcaagttgg taaaatcaaa tttcgtactt tagcccattt acaatatcaa
     4261 taaaacaatg tacagtatat acgaatgcta taaactgaat gttttctcat attaataagg
     4321 aaacattcgg tttttaattt gcatttaaaa ataattagtt tttaaagggg ctatttaaag
     4381 tgaaaatatt tgattacgaa gatattcaat taatacctaa taaatgcata gttgaaagta
     4441 ggtctgaatg tgatacaact atccaatttg gtccgaaaaa attcaagcta cctgtagttc
     4501 ctgcaaatat gcaaacagtt atgaatgaga aattagcgaa atggtttgct gaaaatgatt
     4561 acttttatat catgcatcgt tttgatgaag aagcaagaat accttttata aaacatatgc
     4621 aaaattcagg cttatttgca tctatttcag ttggtgtaaa gaaagcggaa tttgatttta
     4681 ttgaaaagtt agctcaagaa aaattaatcc ccgaatatat tacaatagat attgcgcatg
     4741 gtcactcaga ttcagtgata aacatgatta aacatataaa aaaccatata cctgatagtt
     4801 ttgttattgc tggtaatgtt ggcacgccag aaggtgttag agaattagaa aatgctggtg
     4861 ctgatgctac caaagtcggt ataggtcctg gtagagtttg tattacaaag attaaaacag
     4921 gttttggtac tggtggttgg cagttagcgg cattaaacat atgtagtaaa gcagctcgta
     4981 aacctttgat tgccgatggt ggtataagaa cgcatggcga cattgctaaa tcaattagat
     5041 ttggtgcatc aatggtcatg attggttcat tatttgctgc acacgaagaa tcacctggtg
     5101 aaactgtaga acttgatggt aaacagtata aagaatattt tggtagtgca tctgaatttc
     5161 aaaaaggcga acataaaaat gtagaaggta aaaaaatgtt tgtagaacat aagggttcat
     5221 taatggatac cttaaaagaa atgcaacaag atttacaaag ctcaatttca tatgccggtg
     5281 gaaaagactt gaaatcatta cgtactgtag attatgttat tgttagaaac tctattttca
     5341 acggtgatag agattaatat ttatagtagg tgatgtaaat taaaaaattc atagtaactg
     5401 ttgttgcgtt tttatcaatt actatcattg cgccaataac agaatttaaa ccattcattc
     5461 atttacaaaa tgaagtaaga caatatattg acattcacat caataaagaa acaatttctg
     5521 cggaaaataa attggataca ccgaagaaac aacaatttgc ctttaataat atacaaatga
     5581 acatgtcgaa atcagatgtt gagaaaacat taaataaacc aaaaagagtg acatttaatg
     5641 aatatggtac gaagtggtat acgtattatg atgacgatta caataatttt ataatgataa
     5701 gttacatgaa agataaagtt aatgcgttat atacaaatca aaatataatc acttcaaaat
     5761 caaaaattaa atacaataca cctaaatcgg ttgtaaggca aagattaggc gaaccagaaa
     5821 cagagattgt taaaggtaga gtgcgttacg aacaaaataa taaagaatat gatgttttcc
     5881 ataaaaatca catttatacg acggtatttt atgataagca tcgacgtaat aatgtaacag
     5941 ctgttttaca agtaagtgat gctatggaaa atagattaaa agaacaatat ggagcacctt
     6001 cgaaatcgct tgcagatagt tttgaactac aaaattttga tttagttaat gctgcaagaa
     6061 aacaacatca attatttaca ttgaagtatt ctaaacagaa ttctgaaact gcacgtaagc
     6121 atagtaaaga tatggccaac aatcattatt ttgatcatac aaatttaaaa ggtcaatcac
     6181 catttgatcg attgaaaaaa gatggtatta catttaactc agccggagag aatttagcat
     6241 atggtcaagt tagtagtatc tatgcacatc aaggattaat gaattctatt ggtcacagaa
     6301 aaaatatttt aaatgatacg tttaaaatat taggtgttgg tgttgatttt aatgatgaaa
     6361 aacaaccttt ttggacagaa aattatactg gttaatgttt ttggcatgag attaaagggt
     6421 aatgtttgtc tgtaaggaag tagataaaca tgatgacaac agataaacca aaagatgcgg
     6481 atatacttga acgcgtcaaa gatatactta ataaaaaaga aagaaaaaaa taataatggc
     6541 tcgctcctgt aaattattac gggggcgagt tttaatttgt gtaacatttt gcagaaatct
     6601 gtaattcaaa atttatatta aagaatgaga ttatctaaca attcgcaatt tctgtttgaa
     6661 ctgaatttag attagaataa ataaaacctc ttgaaaacgt aactttttca agaggtccaa
     6721 aatttataaa aatgagggct taacaattac gataatatat aacggtttaa aatattacat
     6781 ttcgcggtac aaaccaatta ctttcccaat tacagcaaca ttgtctaggt aaattggctc
     6841 cattgtacta ttttcaggtt gtaatcgata acgatttttt tctttataga agcgtttgac
     6901 agttgcttca tcttcctcag tcatagcaac aataatgtct ccattttctg ctatggtttg
     6961 actgcgaaca attactttgt ctccgtctaa tataccagcc tcaatcatac tgtcgcctac
     7021 gacgtttaat atgaatatgt cgctattgtg tgtcgatgtt aagtgttcag gtaatggaaa
     7081 atattcttca atattttcta ctgcggtaat aggaacacct gctgtgactt taccaataac
     7141 tggcacatga atcgtttctt ccatattaat attatcattt gtttgatcac ttacaatttc
     7201 tatagcacgt ggtttcgttg gatctcttct tatatagcct ttttcttcaa gacgtgaaag
     7261 gtgaccatga acagttgaac tggatgctaa gccaactgct tcaccaattt cgcgaacact
     7321 aggcggataa cctttcgttt gaacaacttg tttaatatag ttatatattt cgctttgtcg
     7381 ttttgttaat tctctcatat ataggcactc cctaaattaa tttaaataca gtatatcatt
     7441 aatttgaaac caaaacaaac atttgttcgt taaaatattg acacagaaca taagttctga
     7501 taaattacta atacaaacaa atgttctagg agtgaaaatg atgttttaca ataaatataa
     7561 aaacgtatca acatatatca tcatattttt agtttcaagt gcagcctttg caatattctt
     7621 gttaagtgcg aacattagtg ctcactcgga acaagtgtac gaaatgactg accatcaaat
     7681 taagaacaat acgataaata aagcatacga acataaagac cctacaaaca atagcgaaca
     7741 aagagatggg aaagtgttcg ctttaataaa ttgatacatt gtcacaacgt tattttgcct
     7801 atttttgcga aatagcgttt tttattactt ttttgctgat cttaaatttg ttatattttg
     7861 ttaaagtatt ataatgattg aataaacaaa ttgaaggtag gttttttaat tgagtaattc
     7921 tgatttgaat atcgaaagaa ttaacgagtt agctaaaaag aaaaaagaag taggattaac
     7981 tcaagaagaa gcaaaggagc aaacagcctt aagaaaagct tatcttgaga gttttagaaa
     8041 agggtttaaa caacaaattg aaaatactaa agtaattgat ccagaaggta atgatgtaac
     8101 acctgaaaaa attaaagaga tacaacaaaa aagagataat aaaaattaaa tcacaaatct
     8161 gtaaagaatt ttctgacatt ataacttgaa ataagtattt tacttatctt tttattttaa
     8221 aataagttat aatgtatttg ataaaattga agaagggaag atacacaaga tgtttaatga
     8281 aaaagatcaa ttagctgttg atacgctacg tgcactaagt atcgacacaa tcgaaaaagc
     8341 gaattctggt catccaggat tacctatggg agctgcccca atggcttaca ctttgtggac
     8401 acgtcatctg aattttaatc cacaatctaa agattacttc aatagagacc gtttcgtatt
     8461 atctgcaggg catggttcag cattattgta tagcttgtta catgtttctg gtagtttaga
     8521 attagaagaa ttaaagcaat ttagacaatg gggttctaaa acaccaggtc atcctgaata
     8581 cagacataca gatggtgtag aagttactac cggaccactt ggacaaggtt ttgctatgtc
     8641 agtaggatta gctttagcag aagatcacct agcagggaaa tttaataaag aaggatataa
     8701 tgttgtagat cattacacat atgtattagc ttctgacggt gatttaatgg aaggtatatc
     8761 gcatgaagca gcttcatttg ctggacataa taaattaagt aaattagttg ttttatacga
     8821 ttcaaatgat atttcattag atggcgaatt aaacaaagct ttttctgaaa acacaaaagc
     8881 tcgttttgaa gcatatggtt ggaattactt actagttaaa gatggtaatg atttagaaga
     8941 aattgataaa gcgattacta cagctaaatc tcaagaagga ccaacgatta ttgaagttaa
     9001 aacaacaatc ggatttggtt caccgaataa agcaggaact aatggtgttc atggggcacc
     9061 tttaggtgaa gttgaaagaa aattaacatt cgaaaattac ggtttagatc ctgaaaaacg
     9121 ttttaatgtt tcagaagagg tatacgaaat tttccaaaat actatgttaa aacgtgctaa
     9181 tgaagatgaa tctcaatgga attcattatt agaaaaatat gcagaaacat atcctgaatt
     9241 agcagaagaa tttaaattag cgattagtgg taaattgcct aaaaattata aggatgaatt
     9301 accacgtttt gaactgggtc ataatggtgc atctcgtgct gattctggta ctgttattca
     9361 agcaatcagt aaaactgtcc cttcattctt tggtggatca gcagaccttg ctggttcaaa
     9421 caaatccaat gtaaatgatg caacagatta tagttctgaa acacctgaag gtaaaaatgt
     9481 gtggtttggt gtacgtgaat ttgctatggg tgctgctgta aatggtatgg ctgcacatgg
     9541 aggtttacat ccatatggtg caacattctt cgtatttagt gattatttaa aaccagcgtt
     9601 acgtttatca tcaattatgg gattaaatgc aacgttcatc ttcacacatg attcaattgc
     9661 agtaggtgaa gatggtccta ctcatgaacc aattgaacaa ttagctggat taagagctat
     9721 tccaaatatg aatgttatcc gtcctgctga tggtaatgaa acaagagtag catgggaagt
     9781 tgccttagaa tctgaatcta cacctacttc attagtattg acacgtcaaa acttaccggt
     9841 tttagatgta ccagaagatg tagttgaaga aggcgttcga aaaggtgcct atacagttta
     9901 tggctctgaa gagacaccag aattcctatt attagcttca ggttcagaag ttagtcttgc
     9961 agttgaagct gctaaagatc ttgaaaaaca aggtaaatca gtgcgtgttg tttcaatgcc
    10021 taactggaat gcatttgaac aacaatctga agaatataaa gaatcagtta ttccatcaag
    10081 cgtaacaaaa cgtgttgcga ttgaaatggc ttcaccgctt ggatggcata aatatgtagg
    10141 tactgcaggt aaagttattg ctattgacgg ctttggcgca agtgcacctg gcgatttagt
    10201 agttgaaaaa tatggattta ctaaagaaaa tatcttaaac caagttatga gcttataaga
    10261 ataatttata aagcgagtat gtttagaagt ctaggatgca taatcttagg cttcttttaa
    10321 agtgttgaaa tttagagtat agcacttaaa ctacatcata agtgataagt tatgaaagta
    10381 tactatttca gattaatctt taaaagctct gttataacag catgattttt gatattattt
    10441 ttagtatcga tattaaaata cttgaataaa ctagttcttg aaataatgtg atgaatttag
    10501 taaaattcag taggataaag aaagcgggtg aaacaatggc aacttggtta gcaattattt
    10561 ttatagtagc tgcattaatt ttaggtttaa ttggaggttt ccttttagct agaaaatata
    10621 tgatggacta cttgaagaaa aacccaccaa tcaacgaaga aatgcttcgt atgatgatga
    10681 tgcaaatggg tcaaaaacct tctcagaaga aaattaatca aatgatgacg atgatgaata
    10741 aaaatatgga tcaaaatatg aagagtgcga aaaagtaaat tcgcaattga tagaggctat
    10801 tttccagata tggaaatggc ctctttttat aatcaaatta ataagaataa atatgtttat
    10861 taaaattaaa gttaacaaaa tgacgaatag actgagaaat gctataattc attttgtatg
    10921 atttacagag agtttattta acgagaaggt gtctgcgtgc tctatttaat attttcaatc
    10981 attgtagctt tatttatggg aactatagtt atagttattc gtatgaaagc tcaaaattat
    11041 ccggtaaatg agaaaaaaat agttttgcca ccgtttttta tggcgaccgg tgcattgatg
    11101 tacgtcgttc catattttag gctaacagga tcggaaatgc tagaagcctt tataattggt
    11161 ttgctttttt ctacagttct aatttggact tctcgatttg aagtcaaagg tacagaaatt
    11221 tatatgaaac gatctaaagc atttccagtt attttgattt cattacttat cattcgtact
    11281 gtgatgaaaa tattcattag taatgaaata gatcctggag aattaggcgg catgttcttt
    11341 ttattagcat tctgtatgat tgttccttgg agagcagcaa tgctatataa atacaaaaaa
    11401 ctaaagaaaa cattaatcaa ttaattactt ttaaaaccac ttgtgatcga cttctaaatc
    11461 agtcaatgag tggttttaat tttacttgaa aaggtgaaat gattatatta aaataaaagc
    11521 gaacaaatgt tctatatgga ggcagtaatg aaaattatac atacagcaga ctggcactta
    11581 gggaaaatat taaatggcaa acagctttta gaagatcaag cgtatatttt agatatgttc
    11641 gtagaaaaaa tgaaagaaga agaacctgat atcattgtga tagctggaga tttatatgac
    11701 acaacatatc caagtaaaga tgcaatcatg ttattagaac aagcgattgg aaagttaaat
    11761 ttagaactgc gtataccaat aattattatt agtggaaatc acgatggtaa agagaggtta
    11821 aactatgggg cgagttggtt tgaacataat cagttattta taagaacaga ttttacatcg
    11881 attaattcac caatagagat aaatggggtt aatttttata cactccctta tgctactgtg
    11941 agcgaaatga aacactactt tgaagatgac accattgaaa cacatcaaca gggaattacg
    12001 cgctgtattg aaacaatagc accggaaatt gatgaagatg ccgtcaatat tttaattagt
    12061 catctgactg ttcaaggtgg aaagacatct gattctgaaa gaccattaac tattggaacg
    12121 gttgaatcag ttcagaaagg tgtttttgat atatttgatt atgtcatgct aggtcacttg
    12181 catcatccat ttagtataga agacgacaaa attaaatata gtggctcctt attgcagtat
    12241 tcattttcgg aagcgggtca agctaaaggg tatagacgtg taacaattaa tgatggcatt
    12301 attaacgatg tatttattcc tcttaagcca cttagacaat tggaaattat ctcaggcgaa
    12361 tataatgatg ttattaatga aaaagttcat gtgaaaaata aagataatta tttacatttt
    12421 aaacttaaaa atatgtctca tattactgat ccaatgatga gtttaaaaca aatttatcct
    12481 aatactttag cgctgacgaa tgaaactttt aattacaatg aagaaaataa tgcta
//
LOCUS       DAMZKC010000075        12364 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.075, whole genome shotgun sequence.
ACCESSION   DAMZKC010000075 DAMZKC010000000
VERSION     DAMZKC010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12364
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.075"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            214..1152
                     /locus_tag="POB55_001508"
     CDS             214..1152
                     /locus_tag="POB55_001508"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HDI6500473.1"
                     /translation="MANIVFKLSDKDITTLMSRITFDTENLPQGMKARAKYQNTTVNI
                     YQSGKVMFQGNHAEAVSKELLPQHSQLNTNKTKKKNMANSFLEQTLMYDQFNCIGSDE
                     AGSGDYFGPLTVCAAFVTKEHVPILKTLGVDDSKKLTDTKIVELAEQLVTFIPHSLLT
                     LHNEKYNIQQAKGWTQVKMKAVLHNEAIKNVLEKIDSSQLDYIVIDQFAKREVYNHYA
                     LSDIPLPKKTKFETKGESKSLAIAVASIISRYAFVTYMDQISKNINMTIPKGAGAKVD
                     VIAAKIIKKYGLSHLDTISKKHFKNREKAQKILKPL"
     gene            complement(1387..3789)
                     /locus_tag="POB55_001509"
     CDS             complement(1387..3789)
                     /locus_tag="POB55_001509"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HDI6500474.1"
                     /translation="MLISNEWLKEYVTIDDSVSNLAERITRTGIEVDDLIDYTKDIKN
                     LVVGFVKSKDKHPDADKLNVCQVDIGEDEPVQIVCGAPNVDAGQYVIVAKVGGRLPGG
                     IKIKRAKLRGERSEGMICSLQEIGISSNYIPKSFESGIYVFSESQVPGTDALQALYLD
                     DQVMEFDLTPNRADALSMIGTAYEVAALYNTKMTKPDTTSNELELSANDELTVTIENE
                     DKVPYYSARVVHDVTIEPSPIWMQARLIKAGIRPINNVVDISNYVLLEYGQPLHMFDQ
                     DAIGSQQIVVRQANEGEKMTTLDDTERELLTSDIVITNGQTPIALAGVMGGDFSEVKE
                     QTSNIVIEGAIFDPVSIRHTSRRLNLRSESSSRFEKGIATEFVDEAVDRACYLLQTYA
                     NGKVLKDRVSSGELGAFITPIDITADKINRTIGFDLSQNDIVTIFNQLGFDTEINDDV
                     ITVLVPSRRKDITIKEDLIEEVARIYGYDDIPSTLPVFDKVTSGQLTDRQYKTRMVKE
                     VLEGAGLDQAITYSLVSKEDATAFSMQQRQTIDLLMPMSEAHASLRQSLLPHLIEAAS
                     YNVARKNKDVKLFEIGNVFFANGEGELPDQVEYLSGILTGDYVVNQWQGKKETVDFYL
                     AKGVVDRVSEKLNLEFSYRRADIDGLHPGRTAEILLENKVVGFIGELHPTLAADNDLK
                     RTYVFELNFDALMSVSVGYINYQPIPRFPGMSRDIALEVDQNIPAADLLSTIHAHGGN
                     ILKDTLVFDVYQGEHLEKGKKSIAIRLNYLDTEETLTDERVSKVQAEIEAALIEQGAV
                     IR"
     gene            complement(3789..4847)
                     /gene="pheS"
                     /locus_tag="POB55_001510"
     CDS             complement(3789..4847)
                     /gene="pheS"
                     /locus_tag="POB55_001510"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HDI6500475.1"
                     /translation="MSEQQTMSELKQQALVDINEANDERALQEVKVKYLGKKGSVSGL
                     MKLMKDLPNEEKPAFGQKVNELRQTIQNELDERQQMLVKEKLNKQLAEETIDVSLPGR
                     HIEIGSKHPLTRTIEEIEDLFLGLGYEIVNGYEVEQDHYNFEMLNLPKSHPARDMQDS
                     FYITDEILLRTHTSPVQARTMESRHGQGPVKIICPGKVYRRDSDDATHSHQFTQIEGL
                     VVDKNVKMSDLKGTLELLAKKLFGADREIRLRPSYFPFTEPSVEVDVSCFKCKGKGCN
                     VCKHTGWIEILGAGMVHPNVLEMAGFDSSEYSGFAFGMGPDRIAMLKYGIEDIRHFYT
                     NDVRFLDQFKAVEDRGDM"
     gene            complement(5228..5968)
                     /locus_tag="POB55_001511"
     CDS             complement(5228..5968)
                     /locus_tag="POB55_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HDI6500476.1"
                     /translation="MEQITSAQNNRIKQANKLKKKRERDKTGLALIEGVHLIEEAYQS
                     GIVITQLFAIEPARLDQQIIAYAQEVFEINMKVAESLSGTVTPQGFFAIIEKPHYDIS
                     KAQQVLLIDRVQDPGNLGTLIRTADAAGMDAVIMEKGTTDPYQDKVLRASQGSVFHLP
                     VMTQDLDTFITQFNGPVYGTALENAVAYKEVTSSDSFALLLGNEGEGVNPELLAHTTQ
                     NLIIPIYGKAESLNVAIAGSILLYHLKG"
     gene            complement(6208..6315)
                     /locus_tag="POB55_001512"
     CDS             complement(6208..6315)
                     /locus_tag="POB55_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500477.1"
                     /translation="MVLLTFVTTIHDTDDGRFFNEHKHANNKLLKHSLI"
     gene            complement(6352..6675)
                     /gene="isdG"
                     /locus_tag="POB55_001513"
     CDS             complement(6352..6675)
                     /gene="isdG"
                     /locus_tag="POB55_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylobilin-forming heme oxygenase IsdG"
                     /protein_id="HDI6500478.1"
                     /translation="MKFMAENRLTLTKGTAKDIIERFYTRHGIETLEGFDGMFVTQTL
                     EQEDFDEVKILTVWKSKQAFTDWLKSDVFKAAHKHVRSKNEDESSPIINNKVITYDIG
                     YSYMK"
     gene            complement(6694..7428)
                     /gene="srtB"
                     /locus_tag="POB55_001514"
     CDS             complement(6694..7428)
                     /gene="srtB"
                     /locus_tag="POB55_001514"
                     /EC_number="3.4.22.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HDI6500479.1"
                     /translation="MRMKRFLTIVQILLVVIIIIFGYKIVQTYIEDKQERANYEKLQQ
                     KFQMLMSKHQAHVRPQFESLEKINKDIVGWIKLSGTSLNYPVLQGKTNHDYLNLDFER
                     EHRRKGSIFMDFRNELKNLNHNTILYGHHVGDNTMFDVLEDYLKQSFYEKHKIIEFDN
                     KYGKYQLQVFSAYKTTTKDNYIRTDFENDQDYQQFLDETKRKSVINSDVNVTVKDRIM
                     TLSTCEDAYSETTKRIVVVAKIIKVS"
     gene            complement(7490..8455)
                     /gene="isdF"
                     /locus_tag="POB55_001515"
     CDS             complement(7490..8455)
                     /gene="isdF"
                     /locus_tag="POB55_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin ABC transporter permease protein IsdF"
                     /protein_id="HDI6500480.1"
                     /translation="MIKNKKKLLFLCLLVILIATAYISFVTGTIKLSFNDLITKFTTG
                     NNEAVDSIIDLRLPRILIALMVGAMLAVSGALLQAALQNPLAEANIIGVSSGALIMRA
                     LCMLFIPQLYFYLPLLSFIGGLIPFLIIILLHSKFRFNAVSMILVGVALFVLLNGVLE
                     ILTQNPLMKIPQGLTMKIWSDVYILAVSALLGLILTLLLSPKLNLLNLDDIQARSIGF
                     NIDRYRWLTGLLAVFLASATVAIVGQLAFLGIIVPHVVRKLVGGNYRVLIPFSTVIGA
                     WLLLVADLLGRVIQPPLEIPANAILMIVGGPMLIYLICQSQRNRI"
     gene            complement(8468..9346)
                     /gene="isdE"
                     /locus_tag="POB55_001516"
     CDS             complement(8468..9346)
                     /gene="isdE"
                     /locus_tag="POB55_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     IsdE"
                     /protein_id="HDI6500481.1"
                     /translation="MRIIKYLTILVISVVILTSCQSSSSQESTKSGEFRIVPTTVALT
                     MTLDKLDLPIVGKPTSYKTLPNRYKDVPEIGQPMEPNVEAVKKLKPTHVLSVSTIKDE
                     MQPFYKQLNMKGYFYDFDSLKGMQKSITQLGDQFNRKAQAKELNDHLNSVKQKIENKA
                     AKQKKHPKVLILMGVPGSYLVATDKSYIGDLVKIAGGENVIKVKDRQYISSNTENLLN
                     INPDIILRLPHGMPEEVKKMFQKEFKQNDIWKHFKAVKNNHVYDLEEVPFGITANVDA
                     DKAMTQLYDLFYKDKK"
     gene            complement(9343..10419)
                     /gene="isdD"
                     /locus_tag="POB55_001517"
     CDS             complement(9343..10419)
                     /gene="isdD"
                     /locus_tag="POB55_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-regulated surface determinant protein IsdD"
                     /protein_id="HDI6500482.1"
                     /translation="MRNVKQIATKSIIAIISLGILTYTTMIGSVLADEIKYPSAKFNQ
                     PEAKDKTELTTSIFDEKIKENKALELLIFNQENKNVTEEQQLVDEKAQLISDMTGKIY
                     LQVKLKGQIDKEQLVFQNDKNEEFPFVIKDEKDDTIVRILIEQHMDKINMHVKTLAEK
                     KNLDNKEMVYSIHFKEKKVQHDDAKEVPSKHQNQENNQDQLKKDIDDKKDSQKSDTKE
                     RRTSLFTEKGLNDIPVQKDKVQQDSNKKIENERPKASGTLKVENSPPTVKKVENNHKE
                     QPKHKDEKSKKEKKKVVEKEKALPAFNRDDDSKNSSQLSSDIKELDEPNHKKQYILFA
                     AGIVLATILLISAHLYSRKRGNQV"
     gene            complement(10419..11102)
                     /gene="isdC"
                     /locus_tag="POB55_001518"
     CDS             complement(10419..11102)
                     /gene="isdC"
                     /locus_tag="POB55_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme uptake protein IsdC"
                     /protein_id="HDI6500483.1"
                     /translation="MKNILKVFNTTILALIIIIATFSNSANAADSGTLNYEVYKYNTN
                     DTSIANDYFNKPAKYIKKNGKLYVQITVNHSHWITGMSIEGHKENIISKNTAKDERTS
                     EFEVSKLNGKIDGKIDVYIDEKVNGKPFKYDHHYNITYKFNGPTDVAGANAPGKDDKN
                     SASGSDKGSDGTTTGQSESNSSNKDKVENPQTNAGTPAYIYAIPVASLALLIAITLFV
                     RKKSKGNVE"
     gene            11311..12363
                     /gene="isdA"
                     /locus_tag="POB55_001519"
     CDS             11311..12363
                     /gene="isdA"
                     /locus_tag="POB55_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored heme-scavenging protein IsdA"
                     /protein_id="HDI6500484.1"
                     /translation="MTKHYLNSKYQSEQRSSAMKKITMGTASIILGSLVYIGADSQQV
                     NAATEATNATNNQSTQVSQATSQPINFQVQKDGSSEKSHMDDYMQHPGKVIKQNNKYY
                     FQTVLNNASFWKEYKFYNANNQELATTVVNDNKKADTRTINVAVEPGYKSLTTKVHIV
                     VPQINYNHRYTTHLEFEKAIPTLADAAKPNNVKPVQPKPAQPKTPTEQTKPVQPKVEK
                     VKPTVTTTSKVEDNHSTKVVSTDTTKDQTKTQTAHTVKTAQTAQEQNKVQTPVKDVAT
                     AKSESNNQAVSDNKSQQTNKVTKHNETPKQASKAKELPKTGLTSVDNFISTVAFATLA
                     LLGSLSLLLFKRKESK"
BASE COUNT         4007 a         2282 c         1660 g         4415 t
ORIGIN      
        1 aaataatgta aagaattttc tattcaatac tttatcatgt ttaaattgtg tcactataac
       61 attttcataa acattataca tgaccactat gtattttgta agtatccgca attaattctt
      121 tacaacatac ataaatgttt ctgacgttat tatcatttat gatatgatta tttttgctaa
      181 agataatgaa attttatgaa aggatttaca caaatggcga atatcgtttt taaattgtcg
      241 gataaagaca taacgacatt aatgtcacgc attacttttg atactgagaa tttacctcaa
      301 ggtatgaaag cacgtgcaaa gtatcaaaat acaactgtaa atatttacca atcaggtaaa
      361 gtcatgtttc aaggtaatca tgcagaagct gtgtctaaag aacttctacc gcagcattct
      421 caattgaata ctaataaaac taaaaagaaa aatatggcta attcttttct tgaacaaact
      481 ttaatgtatg atcaatttaa ctgtattggt agtgatgaag ctggaagtgg cgattacttt
      541 ggtccattga ctgtatgtgc tgctttcgtt accaaagagc atgtccctat tttaaaaact
      601 ttgggtgtag atgattcaaa aaaattaacc gataccaaaa ttgtagaact tgctgagcaa
      661 ttggttactt tcatcccaca ttcattatta acactacaca atgaaaaata taatattcag
      721 caagcaaaag gctggactca agtaaaaatg aaagctgttc ttcataatga agccatcaaa
      781 aatgtacttg aaaaaattga ttcgtctcaa ttagactata tcgtcattga tcagttcgca
      841 aaacgcgaag tgtataacca ttacgcatta tccgatatac cacttccaaa gaaaacaaaa
      901 tttgaaacaa aaggagaatc gaagtcatta gccattgcag tggcaagcat aatctctcga
      961 tacgcatttg ttacatatat ggatcaaata tcaaaaaata ttaatatgac gattcctaaa
     1021 ggtgccggtg ctaaagtcga tgtaatcgcc gccaaaatta taaaaaaata cggtctttca
     1081 catttagata ccatttcaaa aaagcatttt aaaaatcgtg aaaaagccca aaaaatttta
     1141 aagcctcttt gatttttaat cattataaat tggatcctaa taggaaatcc tattaggtaa
     1201 gtcatactaa tttgctttgc tcataagtat gactttatat gcagttgtgc gaagcatcat
     1261 ctgtattcca ctgttcctct ttgacttttt aaaccaatcg aatcctaatt ggtgaatcca
     1321 aaaataaaac cccatgttag ggatatcaat ctacttcaga tatccttata catggggttt
     1381 aaatcattat ctaataacag caccttgttc aattaatgct gcttcaattt ccgcttgtac
     1441 ttttgaaacg cgctcatctg tcaatgtttc ttctgtgtct aaataattta aacgtattgc
     1501 aattgatttt ttaccttttt ctaaatgttc gccctgatat acatcaaaga caagtgtatc
     1561 ttttaatata ttgccaccgt gtgcatgaat cgttgataat aaatcagctg ctggaatatt
     1621 ttgatctact tctaatgcaa tgtcacgaga catgcctggg aatctcggaa ttggctggta
     1681 attaatgtaa cctaccgaca cagacattaa tgcatcaaaa ttcaactcaa aaacatacgt
     1741 acgttttaaa tcattatcag ctgctaatgt tggatgtaat tcaccaataa aaccaacaac
     1801 tttattttct aataagattt cagctgtacg acctggatgt aatccatcaa tatcggcacg
     1861 acgataacta aattcaaggt ttaacttttc agatactcga tccacgacac cttttgctaa
     1921 atagaaatca accgtttctt tcttaccttg ccattgattg actacataat ctccagttaa
     1981 aataccactt aaatattcaa cttgatctgg tagttcacct tctccattag caaagaagac
     2041 attgccgatt tcaaataatt ttacatcttt atttttgcgt gccacattat atgatgctgc
     2101 ttcgattaaa tgtggtaata aactttgacg taatgacgca tgcgcttcac tcattggcat
     2161 caataaatca attgtttgac gctgttgcat cgaaaatgca gtagcatcct ctttagaaac
     2221 taacgaatac gtaatagctt gatctaatcc agcaccttct aacacttctt taaccattct
     2281 cgttttatat tggcgatcag ttagctgacc actagtaact ttatcgaaga caggtaacgt
     2341 tgatggaata tcgtcgtatc catatatacg tgcaacttct tcaattaaat cttctttaat
     2401 tgtaatatct ttacgacgtg atggtactag cactgtaata acatcatcat ttatttctgt
     2461 atcaaaccct agttgattaa aaatagtaac aatatcattt tgtgacaaat caaatccaat
     2521 agtgcgatta attttatcag cagtgatgtc gattggtgta ataaatgcac caagttctcc
     2581 tgaagacact ctatctttta gcacttttcc gtttgcataa gtttgtaata aataacatgc
     2641 acggtcgact gcttcatcta caaattcagt agctattcct ttttcaaaac gactagatga
     2701 ttcactgcgt aaatttaaac gtcttgatgt atgacgaatt gaaactggat caaaaatagc
     2761 accttcaatc actatatttg atgtttgttc tttaacttct gaaaaatcgc cacccataac
     2821 accagctaat gcaattggag tttgtccatt agtaatgaca atatcgctcg ttaataattc
     2881 acgttctgta tcatctaatg ttgtcatttt ttcgccttca ttagcttgac gaacaacaat
     2941 ttgttgtgaa ccaatcgcat cttgatcaaa catgtgcaat ggttgaccgt attctaataa
     3001 cacataattt gaaatgtcaa caacattatt aataggacgt atacccgctt ttattaagcg
     3061 tgcttgcatc caaattggcg agggttcaat tgtcacgtcg tgaacaacac gtgcactata
     3121 atatggtact ttatcttcat tttctattgt cacagtcagt tcatcatttg cagataactc
     3181 aagctcattt gatgttgtgt ctggcttagt catttttgta ttatacaatg ctgcaacttc
     3241 ataagcagta cctatcatac ttaaagcatc tgcacgattc ggcgttaaat caaattccat
     3301 tacttgatca tctaaatata aagcttgtaa ggcatctgtt cctggaactt gggattcact
     3361 aaatacataa atgcctgatt caaaactttt cggtatataa ttacttgaaa taccaatttc
     3421 ttgtaacgaa caaatcatac cttctgaacg ttcaccgcgt aatttggcac gcttaatttt
     3481 aataccacca ggcaatcttc cacctacttt agcaacaatg acatattgtc ctgcatcaac
     3541 gttcggtgca ccacatacta tttgtacagg ttcgtcttct ccgatatcaa cttggcaaac
     3601 atttaattta tctgcatcag gatgtttatc ttttgactta acgaagccga caactaaatt
     3661 tttgatatct tttgtgtagt caattaaatc atccacttca atacctgtgc gcgtaatacg
     3721 ttctgccaaa ttacttacag aatcatcgat tgttacatat tctttcaacc attcatttga
     3781 tatcaacatt acatgtcacc tctatcttct accgctttaa attgatctaa aaatctcaca
     3841 tcattagtat agaaatgacg aatatcttct ataccatatt tcaacattgc aatacggtct
     3901 ggtcccatac caaatgcaaa tccagagtac tctgaagaat caaaaccagc catttctaat
     3961 acattaggat gtaccattcc agcacctaaa atttcaatcc atcctgtgtg tttacacaca
     4021 ttacaacctt ttcctttaca tttaaaacat gacacatcaa cttctacaga aggttcagtg
     4081 aatgggaagt aacttggacg taaacgaatt tcacgatcag caccaaataa tttcttagct
     4141 aacaattcta aagtaccttt caaatcactc attttaacgt ttttatcaac aactaatcct
     4201 tcgatttgtg taaattgatg actatgtgtc gcatcatcag agtcacgacg atacacttta
     4261 ccagggcaaa taattttaac tggaccttga ccatgacgtg attccatcgt acgtgcctgc
     4321 actggtgatg tatgcgtacg taataaaatt tcatccgtaa tatagaaact atcttgcata
     4381 tcacgtgcag ggtgtgattt aggtaaattc agcatttcga agttataatg atcttgttca
     4441 acttcatatc cattcacaat ttcataacct aaacctaaga ataagtcttc aatttcttct
     4501 attgtacgtg ttaatggatg ctttgaaccg atttcaatat gacgacctgg taatgataca
     4561 tcaattgttt cttcagccaa ttgcttattt aatttttctt taactaacat ctgttgtctt
     4621 tcatctaatt cattttgaat tgtttgacgc aattcattca ctttttgacc aaatgcaggt
     4681 ttctcttcat tcggcaaatc cttcatcaat ttcattagtc cgctaactga ccctttttta
     4741 cctaagtatt tcactttaac ttcttgcagt gcacgttcat catttgcttc attaatatct
     4801 acaagcgctt gttgttttaa ctctgacatt gtttgttgtt cagacatatg tcactgacct
     4861 ccctattcga actattcatt ttatatttat catatttgtt gtttaactaa aaaaagactt
     4921 tcatcctctt aaaaaataag ggacgaaagt catttccgtg gtaccaccca aatttatgcc
     4981 taagcataca cttgtttaat attgataacg gattgtcttt ccgacttaaa tctctttaag
     5041 taaccaaaaa ggtgaaaaga ctttaaataa ttggaataac ttgcagtcac gattattctc
     5101 cctaaaacaa ttatttatat aaatgtccat gcctttttat tggtttatat tcaattgtgt
     5161 atgcaatcac gtagaaatgt tctgttaaac aattaattat aattatatcg gaaaactttc
     5221 aacacggtca acctttcaaa tgataaagta aaatactacc tgcaatcgct acatttaaac
     5281 tttcagcttt accataaata ggtatgatta aattttgtgt agtatgtgct aataattcag
     5341 gattaacacc ttctccctca ttacctaata gtaatgcaaa agaatcactt gaagtaactt
     5401 ctttgtatgc cactgcgttt tcaagtgctg taccataaac aggaccatta aattgagtaa
     5461 taaacgtatc gagatcttgt gtcataactg gcaaatggaa aacactacct tgactcgctc
     5521 gcaacacttt atcttgataa ggatctgtcg tacccttctc cattattaca gcatccattc
     5581 cagcagcatc cgcagttcta attaatgtgc ctaaatttcc aggatcttga acacgatcga
     5641 tgagcaatac ttgttgtgct ttagaaatat catagtgcgg cttctcaatg attgcgaaaa
     5701 acccttgtgg tgtcactgta cctgataaag attcagcaac tttcatgttt atttcaaaaa
     5761 cttcttgcgc gtatgcgata atttgctgat ctaatcttgc cggttcaatt gcaaataatt
     5821 gtgtaattac aattccactt tgataagctt cttcaattaa atgcacacct tcaattaaag
     5881 ctaatccagt tttatccctc tcacgtttct tttttagctt gttcgcttgt ttaattctat
     5941 tattttgtgc agaagtaatt tgttccatag atagctcctc gctttatttt taaaaataaa
     6001 aatattaatc attaataaga tgaaaacatt tgattgtata gttaatatta attaatcgat
     6061 tttatcactc atattatttc aaattgtata aatttctttt atcgatacta ctactataaa
     6121 tcatacgccc caaaatatca ttattaattc ttttcttctt caaaataaat caaaatgata
     6181 taattgatga ttattttcaa agcacattca aatcaaacta tgttttagca atttgttgtt
     6241 agcatgtttg tgttcattaa aaaaacgacc atcatcggta tcatgtatgg tcgttacaaa
     6301 agttaacaat accaattgtc ataacaagta ctgcaacctc tttaaattca attatttcat
     6361 gtaactatag cctatatcat atgtaattac tttgttattg ataatcgggc tactttcatc
     6421 ttcattttta cttctaacat gtttatgtgc tgctttaaag acatcagatt ttaaccaatc
     6481 cgtaaaagct tgctttgatt tccaaactgt taaaattttc acttcatcaa aatcttcctg
     6541 ttctaaagtt tgtgtaacaa acatgccatc aaagccttct aatgtttcaa tcccatgtct
     6601 cgtgtaaaat cgttctataa tatcttttgc tgttcctttt gttaacgtca gcctattttc
     6661 tgccataaat ttcataatta tcctcttttc tgtttaactt accttaatta tttttgcgac
     6721 aacaacaatt cttttcgttg tttcactata tgcatcttcg cacgttgata aagtcattat
     6781 tctatctttt accgttacat taacatctga attaattaca gatttacgtt ttgtttcatc
     6841 taaaaattgt tgataatctt gatcattttc aaaatctgta cgtatgtaat tatctttagt
     6901 agtagtttta tatgcactaa atacttgcaa ttgatattta ccatatttat tgtcaaattc
     6961 aattatcttg tgtttttcat aaaacgattg ctttaaataa tcttctaaca catcaaacat
     7021 cgtattatca ccgacatggt gcccgtataa aatagtatta tgatttaaat tcttcaattc
     7081 atttctaaaa tccataaaaa tactaccttt acgtcgatgt tctcgctcaa aatctaaatt
     7141 taaataatcg tgatttgtct taccttgtag tactggataa tttaatgatg ttcctgataa
     7201 ttttatccat ccaacaatgt ctttatttat tttttcaagt gattcaaatt gtggtctcac
     7261 atgtgcttga tgtttgctca tcagcatttg aaatttttgt tgtaatttct cataatttgc
     7321 gcgttcttgc ttgtcttcaa tatatgtttg aacaattttg taaccaaaaa tgataataat
     7381 tacaaccaat aaaatttgta caatagttaa aaatcgcttc attctcataa aaatcctctt
     7441 ttattaacga cgtttcttca gtcatcacta aaccagttgt tgtaccgttt tagattcgat
     7501 ttcgttgact ttgacaaatt aagtaaatta gcattggacc accgacaatc attaaaatag
     7561 cattggctgg aatttctaga ggaggctgta tcactcgtcc taataaatca gccactaaca
     7621 atagccatgc accaataact gtagaaaacg gaataagtac tctgtaattg cccccaacta
     7681 gctttctaac cacatgtggc acaataatac ctaaaaaggc tagttgtcca acaatcgcaa
     7741 cagttgcact tgctaaaaat actgctaata aacctgttaa ccatctgtaa cgatcaatat
     7801 taaaaccgat acttcgcgct tgtatgtcgt ctaaatttag taaattcaat ttaggggaca
     7861 atagtaatgt taatattaat cccaataatg ctgatactgc taatatgtat acgtcgctcc
     7921 atattttcat tgttaagcct tgaggaattt tcattaaagg gttttgagtt aaaatttcta
     7981 aaacaccatt taataatacg aataacgcaa cacctactaa tatcatactt acagcattga
     8041 atctaaattt agaatgcaac aatataatta ttaaaaatgg tattaaacct ccaataaaac
     8101 ttaataatgg taagtaaaag tacaattgtg gaataaacaa catacaaagt gctctcatta
     8161 taagtgcacc tgaggaaacg ccaatgatat tcgcctctgc caaaggattt tgtagtgctg
     8221 cttgtaataa tgctccagaa actgctaaca ttgcgccaac catcaatgca attaatatac
     8281 gtggcaatcg caaatcaatg attgaatcca ctgcttcatt gttaccagtt gtaaattttg
     8341 taattaggtc attaaatgac aatttaattg taccggttac aaacgaaata taagcagttg
     8401 cgattaaaat gactaacaaa cataaaaata gtagtttctt tttatttttt atcatcatat
     8461 caactcacta ttttttatcc ttataaaata aatcatataa ttgagtcatt gccttatcag
     8521 catcaacatt tgctgtaata ccgaatggca cttcctctaa gtcataaaca tgattatttt
     8581 tcacagcttt aaaatgtttc caaatatcat tctgtttaaa ttctttttga aacattttct
     8641 taacttcttc aggcattccg tgtggtaatc ttaaaataat atctggattg atattcaaca
     8701 aattttcagt attagacgaa atatattgac gatctttcac tttaataaca ttttctccac
     8761 ctgctatttt aactaaatca ccaatatatg atttatcagt tgctactaaa tagctacccg
     8821 gtacacccat taatattaat actttgggat gtttcttttg tttagctgct ttattttcaa
     8881 ttttttgctt tacagaattt aaatggtcat ttaattcttt tgcttgtgct ttacgattaa
     8941 attgatcacc taattgtgta atcgactttt gcatcccttt taaactatca aaatcataaa
     9001 agtagccttt catatttaat tgtttgtaaa atggttgcat ttcatcttta atcgttgaca
     9061 cactcaaaac atgtgttggt tttaattttt taacagcttc aacattcggc tccattggtt
     9121 gaccaatttc cggtacatct ttataacgat taggcaatgt cttatatgac gtgggtttgc
     9181 cgacaattgg taaatccaat ttgtccaatg tcattgtcaa tgcaacagtt gttggtacga
     9241 ttctgaattc gccggattta gttgattctt gagaactgga agattgacag ctggttaaga
     9301 taacgacgct tatcactaaa atggttaaat actttatgat tctcaaactt ggttacctct
     9361 ctttctgctg tataaatgtg ccgaaataag taaaatagtt gctaacacaa tgccagctgc
     9421 aaataaaata tattgctttt tatgatttgg ttcatcaagt tctttaatat cactagataa
     9481 ttgactacta ttcttgctat catcatctct attaaaagct ggtaacgctt tttctttttc
     9541 aactactttt ttcttttcct tttttgattt ttcatcttta tgtttcggtt gctctttgtg
     9601 attattttca acctttttta ctgttggagg gctattttca acttttaatg tacctgatgc
     9661 tttaggtcgc tcattttcaa tctttttatt actgtcttgt tgcactttat ctttttgtac
     9721 aggaatatca tttaatcctt tttcagtaaa aaggctagta cgtctttcct tagtatctga
     9781 tttttgacta tcttttttgt catcaatatc ttttttaagc tgatcttgat tattttcttg
     9841 attttgatgt tttgaaggca cttcttttgc atcatcatgt tgtacttttt tctctttaaa
     9901 atgaatagaa tacaccattt ctttgttatc tagatttttc ttttcagcca acgttttaac
     9961 atgcatattg attttatcca tatgctgttc aattaaaatt cttactattg tgtcatcctt
    10021 ttcatctttt ataacaaaag gaaattcttc atttttgtca ttttgaaaaa caagttgttc
    10081 tttatctatt tgacctttta gctttacttg caagtaaatt ttaccagtca tatctgaaat
    10141 caattgcgcc ttttcgtcaa ctagttgttg ttcctcagtt acatttttat tttcttgatt
    10201 aaaaattagt aactctaacg ctttattctc ttttatcttt tcatcaaaaa ttgatgtagt
    10261 taattctgtt ttatcttttg cttcaggttg attaaatttg gctgatggat attttatctc
    10321 atcagccaac acgctaccaa tcattgttgt atatgtaagt atacctaagc taataatagc
    10381 tataatagat tttgtagcaa tttgtttaac atttctcatt attccacatt gcctttagat
    10441 ttttttctaa caaacaatgt gattgcgatt aataatgcta aggatgcaac tggtattgca
    10501 tatatatatg caggtgtacc agcatttgtt tgtggatttt ctactttgtc tttattcgaa
    10561 ctattagatt cactttgacc agtagtcgtt ccatcagatc ctttgtcact acctgaagca
    10621 gaatttttat catctttacc tggtgcatta gcacctgcta catcagttgg tccattaaat
    10681 ttatatgtaa tgttgtaatg atggtcatat ttgaatggct ttccatttac tttttcatcg
    10741 atataaacgt caatttttcc atctatttta ccgttcaact tacttacttc aaattcagaa
    10801 gtgcgttcat ctttggcagt gtttttacta ataatatttt ctttatgtcc ttcgatactc
    10861 attccagtaa tccaatgact gtggttgaca gttatttgaa catacaattt accatttttc
    10921 ttaatgtact ttgccggttt attaaaatag tcattagcaa ttgacgtgtc attggtattg
    10981 tatttgtaaa cctcataatt caaagtaccg ctatctgcgg catttgcaga attactgaat
    11041 gtcgcgatga tgataattaa cgctaaaatc gttgtattaa aaacttttaa aatatttttc
    11101 aaaacataat cctccttttt atgattgctt ttaagtcttt agtaaaatca taaataataa
    11161 tgattatcat tgtcaatatt tattttataa tcaatttatt attgttatac gaaaatagat
    11221 gtgctagtat aattgataac cattatcaat tgcaatggtt aatcatctca tataacaaca
    11281 cataatttgt atccttagga ggaaaacaac atgacaaaac attatttaaa cagtaagtat
    11341 caatcagaac aacgttcatc agctatgaaa aagattacaa tgggtacagc atctatcatt
    11401 ttaggttccc ttgtatacat aggcgcagac agccaacaag tcaatgcggc aacagaagct
    11461 acgaacgcaa ctaataatca aagcacacaa gtttctcaag caacatcaca accaattaat
    11521 ttccaagtgc aaaaagatgg ctcttcagag aagtcacaca tggatgacta tatgcaacac
    11581 cctggtaaag taattaaaca aaataataaa tattatttcc aaaccgtgtt aaacaatgca
    11641 tcattctgga aagaatacaa attttacaat gcaaacaatc aagaattagc aacaactgtt
    11701 gttaacgata ataaaaaagc ggatactaga acaatcaatg ttgcagttga acctggatat
    11761 aagagcttaa ctactaaagt acatattgtc gtgccacaaa ttaattacaa tcatagatat
    11821 actacgcatt tggaatttga aaaagcaatt cctacattag ctgacgcagc aaaaccaaac
    11881 aatgttaaac cggttcaacc aaaaccagct caacctaaaa cacctactga gcaaactaaa
    11941 ccagttcaac ctaaagttga aaaagttaaa cctactgtaa ctacaacaag caaagttgaa
    12001 gacaatcact ctactaaagt tgtaagtact gacacaacaa aagatcaaac taaaacacaa
    12061 actgctcata cagttaaaac agcacaaact gctcaagaac aaaataaagt tcaaacacct
    12121 gttaaagatg ttgcaacagc gaaatctgaa agcaacaatc aagctgtaag tgataataaa
    12181 tcacaacaaa ctaacaaagt tacaaaacat aacgaaacgc ctaaacaagc atctaaagct
    12241 aaagaattac caaaaactgg tttaacttca gttgataact ttattagcac agttgccttc
    12301 gcaacacttg cccttttagg ttcattatct ttattacttt tcaaaagaaa agaatctaaa
    12361 taaa
//
LOCUS       DAMZKC010000076        12344 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.076, whole genome shotgun sequence.
ACCESSION   DAMZKC010000076 DAMZKC010000000
VERSION     DAMZKC010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12344
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.076"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(182..1084)
                     /gene="cvfB"
                     /locus_tag="POB55_001520"
     CDS             complement(182..1084)
                     /gene="cvfB"
                     /locus_tag="POB55_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HDI6500485.1"
                     /translation="MALDKDIVGSIEFLEVVGLQGSTYLLKGPNGENVKLNQSEMNDD
                     DELEVGEEYSFFIYPNRSGELFATQNMPDITKDKYDFAKVLKTDRDGARIDVGLPREV
                     LVPWEDLPKVKSLWPQPGDHLLVTLRIDRENHMYGRLASESVVENMFTPVHDDNLKNE
                     VIEAKPYRVLRIGSFLLSESGYKIFVHESERKAEPRLGESVQVRIIGHNDKGELNGSF
                     LPLAHERLDDDGQVIFDLLVEYDGELPFWDKSSPEAIKEVFNMSKGSFKRAIGHLYKQ
                     KIINIETGKITLTKKGWSRMDSKE"
     gene            1231..2832
                     /locus_tag="POB55_001521"
     CDS             1231..2832
                     /locus_tag="POB55_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDI6500486.1"
                     /translation="MLQVTDVSLRFGDRKLFEDVNIKFTEGNCYGLIGANGAGKSTFL
                     KILSGELDSQTGHVSLGKNERLAVLKQDHYAYEDERVLDVVIKGHERLYEVMKEKDEI
                     YMKPDFSDEDGIRAAELEGEFAEMNGWNAEADAANLLSGLGIDPTLHDKKMAELENNQ
                     KIKVLLAQSLFGEPDVLLLDEPTNGLDIPAISWLEDFLINFDNTVIVVSHDRHFLNNV
                     CTHIADLDFGKIKVYVGNYDFWYQSSQLAQKMAQEQNKKKEEKMKELQDFIARFSANA
                     SKSKQATSRKKQLEKIELDDIQPSSRRYPFVKFTPEREIGNDLLIVQNLSKTIDGEKV
                     LDNISFTMNPNDKAILIGDSEIAKTTLLKILAGEMEPDEGSYKWGVTTSLSYFPKDNS
                     EFFEGVNMNLVDWLRQYAPEDEQTETFLRGFLGRMLFSGEEVKKKASVLSGGEKVRCM
                     LSKMMLSSANVLLLDEPTNHLDLESITAVNDGLKSFKGSIIFTSYDFEFINTIANRVI
                     DLNKQGGVSKEIPYEEYLQEIGVLK"
     gene            3699..4904
                     /locus_tag="POB55_001522"
     CDS             3699..4904
                     /locus_tag="POB55_001522"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HDI6500487.1"
                     /translation="MVTRSVLKYGGSSVSDFTKIKRIAEMLKERVNQDEQLIVVVSAM
                     GNTTDQLMTNVSTLTKAPKQQELALLLTTGEQQTVSYLSMVLNDIGVNAKAMTGYQAG
                     IKTIGHHLKSKIAQINPQTFEQAFQENDILVVAGFQGINEHQELTTLGRGGSDTTAVA
                     LAVSNQIPCEIYTDVDGVYATDPRLLPKAKRLDIVSYEEMMEMSALGAGVLETRSVEL
                     AKNYNIPLYLGKTLSNVKGTWIMSNEEILEKKAVAGVALDKHMMHVTISYPLPDNQLL
                     TQLFTELEEGAVNVDMISQIVNLDGLQLSFTIKDSDFHQISMILETLKNQYEALAYKI
                     NEHYVKISLIGSGMRDMSGVASKAFLTLIENNIPFYQTTTSEISISYVIDDFNGQQAV
                     EKLYDAFNI"
     gene            4968..5957
                     /locus_tag="POB55_001523"
     CDS             4968..5957
                     /locus_tag="POB55_001523"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDI6500488.1"
                     /translation="MTKLAVVGSTGLVGTKMLETLNRKDIPFDELVLFSSARSAGKEV
                     EFQGKTYTVQELTDARASEHFDYVLMSAGGGTSEHFAPLFEKAGAIVIDNSSQWRMAE
                     DIDLIVPEVNEPTFTRGIIANPNCSTIQSVVPLKVLQDAYGLKRVAYTTYQAVSGSGM
                     KGKKDLAEGVNGKAPEAYPHPIYNNVLPHIDVFLENGYTKEEQKMIDETRKILNAPDL
                     KVTATCARVPVQDSHSVEIDVTLEKEATAEDIKALFDQDDRVVLVDNPENNEYPMAIN
                     STNKDEVFVGRIRRDDSLENTFHVWCTSDNLLKGAALNAVQVLEQVMRLKGAN"
     gene            5959..6846
                     /locus_tag="POB55_001524"
     CDS             5959..6846
                     /locus_tag="POB55_001524"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDI6500489.1"
                     /translation="MTHLFEGVGVALTTPFTNNKINIEALKTHVNFLLENNAQAIIVN
                     GTTAESPTLTTDEKERILKTVIDLVDKRVPVIAGTGTNDTEKSIQASIQAKALGADAI
                     MLITPYYNKTNQRGLVKHFEAIADAVKLPVVLYNVPSRTNMTIEPETVEILSQHPYIV
                     ALKDATNDFEYLEEVKKRIDTNSFALYSGNDDNVVEYYQRGGQGVISVIANVIPKEFQ
                     ALYDAQQSGLDIQDQFKPIGTLLSALSVDINPIPIKALTSYLGFGNYELRLPLVSLED
                     TDTKVLREAYDTFKAGENE"
     gene            6843..7565
                     /locus_tag="POB55_001525"
     CDS             6843..7565
                     /locus_tag="POB55_001525"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDI6500490.1"
                     /translation="MKILLIGYGAMNQRVARLAEEKGHEIVGVIENTPKATTPYQQYQ
                     HIADVKDADVAIDFSNPNLLFPLLDEEFHLPLVVATTGEKEKLLNKLDELSQNIPVFF
                     SANMSYGVHALTKILAAAVPLLDEFDIELTEAHHNKKVDAPSGTLEKLYDVIVSLKEN
                     VTPVYDRHELNEKRQPQDIGIHSIRGGTIVGEHEVLFAGTDETIQITHRAQSKDIFAN
                     GAIQAAERLVNKPNGFYTFDNL"
     gene            7592..8311
                     /gene="dapD"
                     /locus_tag="POB55_001526"
     CDS             7592..8311
                     /gene="dapD"
                     /locus_tag="POB55_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HDI6500491.1"
                     /translation="MVQHLTAEEIIQYISDAKKSTPIKVYLNGNFEGITYPESFKVFG
                     SEQSKVIFCEADDWKPFYEAYGSQFEDIEIEMDRRNSAIPLKDLTNTNARIEPGAFIR
                     EQAIIEDGAVVMMGATINIGAVVGEGTMIDMNATLGGRATTGKNVHVGAGAVLAGVIE
                     PPSASPVIIEDDVLIGANAVILEGVRVGKGAIVAAGAIVTQDVPAGAVVAGTPAKVIK
                     QASEVQDTKKEIVAALRKLND"
     gene            8454..9605
                     /locus_tag="POB55_001527"
     CDS             8454..9605
                     /locus_tag="POB55_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDI6500492.1"
                     /translation="MNELEFVTKHRRHLHQHPELSLHEFETTAYIKAFLDSLNIKYDC
                     PLETGVIAYLEGNGSHTIAYRADIDALPILEENDVPYRSQSDHVMHACGHDGHTTALM
                     LFVQRCKDMQDAGQLPQNVVFIFQPAEETGGGANRLIKAGAFDKYPIEAVFGIHVNPF
                     ADEGIAVIRDEEITASATEYRFFLTGLSSHVADKEQGHSCGEALQHVLTQISQIQQFH
                     LNGLKRNIVHIGHFKAGEAINTVPSNGYLEGTIRTYDIDDLTIVKNQMHKIAESVKLL
                     FNVDCEVKFAEDYPPTINSPKLRTQIEDALIKADLNVYDKPTPFLFGEDFSFYGQQLA
                     PAYFVFIGTRNEDKGFVTGLHTSHLNFDEKVLINVVNFYENLLNNYKEV"
     gene            9610..10695
                     /locus_tag="POB55_001528"
     CDS             9610..10695
                     /locus_tag="POB55_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDI6500493.1"
                     /translation="MTATWSVNKKIFLQNAITVKNNQPLMAVVKNNAYHYDLEFAVTQ
                     FIHAGIDTFSTTSLREAIQIRQLVPDATIFLMNAVYEFDLVREHQIHMTLPSLTYYYN
                     HKNDLAGIHVHLEFENLLHRSGFKDLNEIKEVLKDHHHNQNAKMIISGLWTHFGYADE
                     FDVSDYNVERSQWMEIVEALLSEGYQFDLIHAQNSASFYREGQILLPHHTHARVGIAL
                     YGSRPYSSLNQHDIVQSLTVKAHVIQVREVQAGDYCGYSFAFEVTKNNTKLAVVDIGY
                     GDGILRTRAKHEALINGKRYPIRALMMSHMFVEVDGNVHAQDEVILYNNDIRIDEYTF
                     KGVGANSEQLSAMNHDSLKKEYISNDC"
     gene            10685..11950
                     /gene="lysA"
                     /locus_tag="POB55_001529"
     CDS             10685..11950
                     /gene="lysA"
                     /locus_tag="POB55_001529"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HDI6500494.1"
                     /translation="MTVKYNQNGELTMDGISLKTIAQSFGTPTIVYDELQIREQMRRY
                     HRAFKDSGLKYNISYASKAFTCIQMVKLVAEEDLQLDVVSEGELYTALEAGFEPSRIH
                     FHGNNKTKHEIRYALENNIGYFVIDSLEEIELIDRYANDTVQVVLRVNPGVEAHTHEF
                     IQTGQEDSKFGLSIQYGLAKKAIDKVQQSKHLKLKGVHCHIGSQIEGTEAFIETAKIV
                     LRWLKEQGIQVELLNLGGGFGIKYVEGDESFPIESGIKDITDAIKSEIKVLGIDAPEI
                     GIEPGRSIVGEAGVTLYEVGTIKEIPEINKYVSIDGGMSDHIRTALYDAKYQALLVNR
                     NEEADDSVTIAGKLCESGDIIIKDAKLPSSVKRGDYLAILSTGAYHYSMASNYNQMQK
                     PSVFFLKDGKAREVIKRQSLRQLIINDTK"
     gene            complement(12190..>12344)
                     /gene="msa"
                     /locus_tag="POB55_001530"
     CDS             complement(12190..>12344)
                     /gene="msa"
                     /locus_tag="POB55_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sarA expression modulator protein Msa"
                     /protein_id="HDI6500495.1"
                     /translation="IKAEMMELEDIDVLITSKDFGFDEILFYTLYLLLILIVLYYLKK
                     QVKHKI"
BASE COUNT         4295 a         1937 c         2256 g         3856 t
ORIGIN      
        1 atttccatca aataattaat agaaatcagc ttttttacat tgcctaagaa cttaatgtcc
       61 cagcctattc gtttgaatta tttaattttt tactttgaaa ggaatgttta aaaaactact
      121 tcatctgtcc gatttaatat tgaataaaaa aacacgcatc ctacgacacg tgtaaaaatg
      181 attattcttt tgagtccatt cgactccaac cttttttagt taaagtgatt ttacctgttt
      241 ctatattaat aatcttctgt ttatataagt gaccgattgc acgtttgaat gaacctttac
      301 tcatattgaa tacttcttta atcgcttcag ggcttgattt gtcccagaat ggtaattcac
      361 catcatattc aactagtaaa tcaaagatga cttggccgtc atcgtctaaa cgttcatgtg
      421 caagtggtaa aaatgaacca tttaactcac ctttatcatt atgcccgata attctaactt
      481 gaacagattc acctaatctt ggttcagctt tacgttctga ttcatgtacg aaaattttgt
      541 aacctgattc gcttaataaa aagctaccaa ttcgtaatac gcggtaaggt ttggcttcaa
      601 tgacttcgtt ttttaaatta tcgtcgtgta caggtgtaaa catattttct acaacagatt
      661 cactcgctaa tcttccatac atatgattct cacggtcaat tcgtaatgtg actagcaaat
      721 gatcaccagg ttgtggccat agtgatttca cttttggtaa atcttcccat ggtactaaca
      781 cttcacgggg taatccaaca tctatacgtg ccccatcgcg atccgtttta agtactttag
      841 caaagtcata tttatctttc gtaatatcag gcatattttg agttgcaaat aattcacctg
      901 aacggtttgg ataaatgaag aaactatatt cttcacctac ttctaattca tcatcatcgt
      961 tcatttctga ttggtttaac tttacgtttt caccgtttgg tccttttaaa aggtaagttg
     1021 agccttgtaa ccctactact tcaaggaatt ctatagaacc tactatatct ttgtctaatg
     1081 ccatgttatt ctccttcgat tcttgtattc tttattatta taacatgtgt gagtcattct
     1141 atcattacaa cttatatgag gtcgaaactg tgacgataat atgatataat tcttccgtta
     1201 gaatatcgaa tgaaaaaaag gagaaaatgc atgttacaag taactgatgt gagtttacgt
     1261 tttggagatc gtaaactatt tgaagatgta aatattaaat ttacagaagg taattgttat
     1321 ggattaattg gtgcgaatgg tgcaggtaaa tcaacattct taaaaatatt atctggtgaa
     1381 ttagattctc aaacaggaca tgtttcatta ggtaaaaatg aacgtctagc tgttttaaaa
     1441 caggaccact atgcttatga agatgaacgc gtgcttgatg ttgtaattaa aggtcacgaa
     1501 cgtctttatg aggttatgaa agaaaaagat gaaatctata tgaagccaga tttcagtgat
     1561 gaagatggta tccgtgctgc tgaacttgaa ggtgaatttg cagaaatgaa tggttggaat
     1621 gctgaagctg atgctgctaa ccttttatct ggtttaggta tcgatccaac tttacacgat
     1681 aaaaaaatgg ctgaattaga aaacaaccaa aaaattaaag tattattagc gcaaagttta
     1741 ttcggtgaac cagacgtact attactggat gagcctacta acggtctcga tattccagca
     1801 atcagttggt tagaagattt cttaattaac tttgataata ctgttatcgt agtatcgcat
     1861 gaccgtcatt tcttaaataa cgtatgtaca catatcgctg atttagactt tggtaaaatt
     1921 aaagtttatg ttggtaacta tgatttttgg tatcaatcta gtcagttagc tcaaaagatg
     1981 gctcaagaac aaaacaagaa aaaagaagaa aaaatgaaag agttacagga ctttattgct
     2041 cgtttctcag ctaacgcttc taaatctaaa caagcaacaa gtcgtaaaaa acaacttgag
     2101 aaaattgaat tagatgatat tcaaccatca tcaagaagat atcctttcgt taaattcacg
     2161 cctgagcgtg agattggtaa tgacttatta atcgttcaaa atctttctaa aacgattgac
     2221 ggtgaaaaag tattagataa catttcattc acaatgaatc caaatgataa agcaatttta
     2281 attggagata gtgaaatcgc gaaaacaaca ttacttaaaa tattagctgg tgaaatggaa
     2341 ccagacgaag gttcttataa atggggtgta acaacatcat taagttactt ccctaaagat
     2401 aactcagaat tctttgaggg cgttaatatg aaccttgtag attggttaag acaatatgca
     2461 ccggaagatg aacaaacaga aacattttta cgtggtttct taggtcgtat gttatttagt
     2521 ggtgaagaag ttaagaaaaa agctagtgtg ctttcaggtg gagaaaaagt acgttgtatg
     2581 ctaagtaaaa tgatgttatc aagtgcgaat gtacttttac ttgacgaacc tactaaccac
     2641 ttagacttag aaagtattac tgctgtcaat gatggtctta aatcatttaa aggttctatc
     2701 atctttactt cttatgactt cgaatttatc aacacgattg caaaccgtgt tatcgattta
     2761 aataaacaag gcggcgtttc aaaagaaatt ccatatgaag aatacttgca agaaatcggc
     2821 gttttaaaat aaacataata tgtagaattt cttttcgaaa atctctgtgt tgaggcccga
     2881 ccaacttgca cattattgta agttggctta ctgtcacctt ctatattggg acttattccg
     2941 caacttgtat tgtttgtaga aattaggaat ccaatttctc tatgtgtggc accgacaagg
     3001 attgaaaaaa gctagctaca aagtcatttt cattcagtca actactgtca atataacatt
     3061 gcagggccta gggcattgat tcatgtccga gactatattc aatttaatag acattaactg
     3121 ggcgtctcga tgctaatatt tatagcatca aggtgtcctt tttattttat tttttagcac
     3181 atcagcacag tgaacacccc ttctctaaat tacaaataca gttatcaccg cattaaaaga
     3241 gtacatttat ttaatgtgca tattgagtat ggagtatgct ataagtattt tgaattttat
     3301 ttttcaaaaa gtctcattat caccaccttt taaaatataa gcaacgctat aacttgaatt
     3361 ttcaggttat tccaaaaaat ctaaattatc ggtttacatt catatttgtc atatgtataa
     3421 tgaacacata ccaatttaat aatattttga tgaggcgcat caatcatgag taaagtttag
     3481 attactgtct gctaacagct aaatttgaaa gggtgcgatg ccgaagcgat tataatagca
     3541 gttataattt gttggacttt ttggttaaga gctgagagtt tgtcattatt taaaaataat
     3601 ggagtgcatc acttgtatat agattaagag caagttcgca ttccgaactt gttctttttt
     3661 tattattgtg tgcccttcct aacaattagg aggattatat ggtaacaaga agtgtgttga
     3721 aatatggcgg atcatccgtc agtgatttta caaaaataaa aaggatcgct gaaatgttaa
     3781 aggagcgggt caatcaagat gaacaattaa ttgtcgttgt aagtgctatg ggtaacacaa
     3841 cagatcaatt aatgacgaat gtatcgacct tgactaaagc accaaaacaa caagaactgg
     3901 cattattatt gacaaccgga gagcaacaaa ctgtatctta tttatcaatg gtattaaatg
     3961 atatcggtgt gaatgccaaa gcaatgactg gctatcaagc gggtattaaa accattggcc
     4021 atcatttaaa aagtaaaatt gctcaaatta atcctcaaac atttgaacaa gcctttcaag
     4081 aaaacgatat tttagtagtt gctggatttc aaggcatcaa tgaacatcag gaattaacaa
     4141 ctttaggcag aggcggttct gatacgaccg ctgtggcact tgctgttagt aatcaaatac
     4201 cttgtgaaat ttataccgac gttgatggtg tgtatgccac tgacccaaga cttttaccaa
     4261 aagctaaacg actagacatc gtctcatatg aagaaatgat ggaaatgagc gctttaggtg
     4321 ctggtgtact tgaaacaaga agtgttgaat tagctaaaaa ctataatatc cctttatatt
     4381 taggaaaaac tttatcgaac gtgaaaggaa catggattat gtcaaatgaa gaaatattag
     4441 agaaaaaagc agttgctggt gtggctttgg ataaacatat gatgcatgta acaattagtt
     4501 atcccctacc tgacaatcag ctacttaccc aactatttac ggaacttgaa gaaggtgctg
     4561 taaatgttga tatgatttca caaatcgtca acttggatgg actacaacta tccttcacga
     4621 ttaaagatag tgattttcat caaatttcta tgattcttga aacattaaag aatcaatatg
     4681 aagcattagc ttataaaatc aatgagcatt atgtcaaaat ttcattaatt ggctcaggca
     4741 tgcgtgatat gtcaggtgtg gcatcaaaag catttttgac attaattgaa aataatatac
     4801 ctttctacca aacaacaact tctgaaataa gtatttcata cgtcattgat gattttaatg
     4861 ggcaacaagc ggtggaaaaa ctatatgacg catttaacat ttaatggtaa aatgattgtt
     4921 aaaatattct aaaaattgga aattattata aaatggagtg acaagttatg acaaaattag
     4981 cagttgtggg ttcaacagga ttagtaggta caaaaatgtt ggagacatta aatcgtaaag
     5041 atattccttt cgatgaatta gtattatttt catcagcacg ttctgcaggg aaagaagttg
     5101 aatttcaagg aaaaacatat acagttcaag aattaactga tgctcgtgca agtgaacatt
     5161 tcgattatgt attaatgagt gctggtggcg gtacaagcga acactttgcc ccactctttg
     5221 aaaaagctgg tgcaatcgtt atagacaatt caagtcaatg gcgtatggca gaagatattg
     5281 atttaatcgt tccggaagtc aatgaaccta cttttacaag aggtatcatt gccaatccaa
     5341 actgctctac gattcagtct gttgtgccat taaaagtatt gcaagatgct tatggtttaa
     5401 aacgagtggc atatacaaca tatcaagctg tatcaggttc agggatgaaa ggtaagaaag
     5461 atttagctga aggtgtaaat ggtaaagcac cagaagcata tccacatcca atttataata
     5521 atgtgttacc gcatattgat gtgtttttag aaaacggata tacaaaagaa gaacaaaaaa
     5581 tgattgatga gacgagaaaa attttaaatg cgccagactt aaaagtaaca gcaacatgtg
     5641 cacgtgtgcc tgttcaagat agtcatagtg ttgaaattga tgtaacgctt gaaaaagaag
     5701 cgactgcaga agatattaaa gcattatttg atcaagatga ccgcgttgtt ttagtagaca
     5761 atccagagaa caatgaatat ccaatggcaa tcaattctac taataaagat gaagtgtttg
     5821 ttggccgtat acgtagagat gattcattag aaaatacttt ccatgtatgg tgtacatcag
     5881 acaatttatt aaaaggtgct gcattaaatg ctgtacaagt attggaacaa gttatgcgtt
     5941 taaaaggagc gaattaaaat gacacattta tttgagggtg ttggcgttgc acttacaacc
     6001 ccttttacaa ataacaaaat taatattgaa gctttgaaaa cacacgttaa ttttttacta
     6061 gaaaataatg cccaagcaat catcgttaat ggaactactg ctgagagccc tactttaaca
     6121 acagatgaaa aagaacgcat tctaaaaaca gttattgatc ttgtagataa acgtgttcct
     6181 gtcatagcag gaactggcac taatgatact gaaaagtcaa tccaagcttc aatccaagct
     6241 aaagccttag gggctgatgc aattatgtta attacgccct actacaacaa aacgaatcaa
     6301 cgtggtttag tcaaacactt tgaagcgatt gcagatgctg tgaaattacc agtcgtgctg
     6361 tacaatgttc cttcaagaac gaacatgaca attgaaccag aaactgtaga aatattaagt
     6421 caacatcctt atatagttgc tttaaaagat gctacgaatg attttgagta tttagaagaa
     6481 gtgaaaaagc gaattgatac aaattcattt gcattatata gtggcaatga tgacaacgtc
     6541 gttgaatact atcaacgtgg cggtcaaggg gttatctctg ttattgccaa tgtcattcct
     6601 aaagaatttc aagcgttata cgatgctcaa caaagtggat tagatattca agatcaattt
     6661 aaaccaatcg gcacactgtt atcagcccta tcagttgata ttaacccaat tcctattaaa
     6721 gctttaacaa gttatttagg atttggaaat tatgaattac gtttaccatt ggttagccta
     6781 gaagatacag atactaaagt gcttcgtgaa gcatatgaca catttaaagc gggtgaaaat
     6841 gagtgaaaat attactaatt ggctatggtg caatgaatca acgcgttgct agattagcag
     6901 aagaaaaagg acatgaaatc gttggggtca ttgaaaatac accgaaagca acaacgccat
     6961 atcaacaata tcaacatatt gcagatgtta aagatgccga tgttgcaata gatttttcaa
     7021 atccaaatct gcttttccct ttattagatg aagagtttca tttgccatta gttgtggcaa
     7081 caactggcga gaaagaaaaa ctacttaata agttagatga attgagtcaa aatatacctg
     7141 tatttttcag cgcgaacatg agttatggcg ttcatgcact gactaaaatt ttagcagctg
     7201 ctgttcccct acttgatgaa ttcgacatcg aattaactga ggcacatcat aataaaaaag
     7261 tagatgcacc aagtggtacg ttagaaaaat tgtatgatgt gatcgtatct ttgaaagaaa
     7321 atgtaacacc tgtgtatgat agacatgaat taaatgaaaa acgccagcca caagatattg
     7381 gtatacattc tattcgtgga ggtacgattg tcggtgaaca tgaagttcta tttgctggca
     7441 ctgatgaaac gattcaaatc acgcatcgtg cacaatcaaa agatattttt gcgaatggtg
     7501 caatacaagc agcagaacgc ttagttaata aaccaaacgg cttttatacg tttgacaact
     7561 tataaacata ttaaaggaga ttgattattt tatggtacaa catttaacag ctgaagaaat
     7621 tattcaatat ataagtgatg ctaaaaagtc tacaccaata aaagtatatt taaatggtaa
     7681 ttttgaaggc atcacatatc cagaaagttt taaagtattt ggttcagaac aatctaaagt
     7741 aatcttttgt gaagcggatg attggaaacc tttttacgaa gcatatggta gtcaattcga
     7801 agatatagaa attgaaatgg atcgtcgcaa ttctgccatt ccattaaaag acttaacaaa
     7861 tacgaatgca cgaattgaac caggtgcgtt tattagagaa caagccatta ttgaagatgg
     7921 tgctgtcgtt atgatgggcg caacaattaa tattggcgca gtcgttggcg aaggtacaat
     7981 gattgatatg aatgctactc tcggtggtcg tgctacaact ggtaaaaatg tacatgtagg
     8041 ggctggcgca gtattagcag gtgtgattga accccctagt gcatcaccgg ttataatcga
     8101 ggatgatgta ttaatcggtg caaatgcagt tattttagaa ggtgtacgtg ttggtaaagg
     8161 tgctattgtt gcagctggcg cgattgtgac acaagatgta ccagctggtg cagttgttgc
     8221 tggtacacct gcaaaagtga ttaagcaagc ttctgaagta caagatacta aaaaagagat
     8281 tgtagcagca ttaagaaaac tgaatgacta gtgacgtcaa cgtataataa tttcaaggtt
     8341 gagatacgat tatgtctcaa ccttattgtt tatcattgaa taaacttatg atcacaaatt
     8401 tcgaaatacg ttacactatt tatatagtaa tttaacacaa aggattgata attatgaatg
     8461 aattagaatt tgttacgaaa catcgccgtc atttacatca acatcctgaa ttaagcttac
     8521 atgaatttga aacaactgct tatattaaag cgtttttaga tagtttaaat attaaatacg
     8581 attgcccatt ggaaactggc gtcattgcat acttagaagg taatggctca catacgatag
     8641 cgtatagagc tgatattgat gccttaccta ttttagagga aaatgatgtg ccttatcgca
     8701 gtcaatctga tcatgtgatg catgcttgtg gacatgatgg tcatacaact gcattaatgc
     8761 tttttgtaca acgttgcaaa gacatgcaag acgcaggtca attaccgcaa aatgtcgttt
     8821 tcattttcca acctgcagaa gaaactggtg gcggtgcaaa tcgattaata aaagccggtg
     8881 cctttgataa gtatccaatt gaagcggtat ttggtattca tgttaaccca tttgctgatg
     8941 aaggcattgc agtgataaga gatgaagaaa ttacggccag cgcaacagag tatcgctttt
     9001 tcttaacagg cctgtcaagt catgttgctg ataaagaaca aggtcattct tgtggtgaag
     9061 cattacaaca tgtattaact caaatatcac aaattcaaca atttcacctt aacggtttga
     9121 aacgaaatat tgttcatatt ggtcatttta aagctggtga agcgattaac actgtaccaa
     9181 gtaatggtta tttagaaggt acaattcgta catatgatat tgatgattta acaatcgtta
     9241 aaaatcaaat gcacaagata gcagaaagtg tcaagcttct gtttaatgta gattgtgaag
     9301 ttaaatttgc agaagattat ccccctacaa tcaatagtcc gaaattacgt actcaaatag
     9361 aggacgcctt aataaaagct gatttaaatg tctatgacaa accaacgcca ttcttatttg
     9421 gggaagattt tagtttttat ggtcaacaac tagctccagc ttactttgtt tttataggaa
     9481 cacgaaatga agataaaggt tttgtaactg gtttgcacac atcacattta aattttgatg
     9541 aaaaagtgtt aataaacgtg gttaattttt acgaaaattt attaaataat tacaaagagg
     9601 tgtaatacat tgacagcaac atggtctgta aataagaaaa tatttttaca aaatgcaatc
     9661 acagtcaaaa acaatcagcc attaatggca gttgttaaaa ataatgcata tcactatgac
     9721 ctagaatttg ctgtaactca gtttatccat gcaggtatag atacatttag cacaacatca
     9781 ctacgagaag caattcaaat tagacaactt gttccagatg caacaatctt tttaatgaat
     9841 gcagtttacg agtttgattt agtccgtgaa catcaaatac acatgacttt gccgtcgttg
     9901 acatattact ataaccataa aaatgattta gcaggtattc atgttcactt agaatttgaa
     9961 aatttattac atcggtctgg atttaaagat ttaaacgaaa ttaaagaagt attgaaagat
    10021 caccatcata atcaaaatgc aaaaatgatt attagtggtt tatggaccca ttttggatat
    10081 gctgatgaat tcgatgtgtc agattataat gttgaacgtt cacaatggat ggaaattgtt
    10141 gaagcacttt tatctgaagg ttatcagttc gacctaatcc atgctcaaaa tagtgcgagt
    10201 ttttatcggg aaggacaaat attactaccc caccatacac atgcacgtgt aggtattgcg
    10261 ttatacggtt caagaccata tagttcactg aatcaacatg atatagttca gtcattaact
    10321 gtaaaagcac atgttattca agtgcgcgaa gtacaagctg gtgattattg cggttatagc
    10381 tttgcctttg aagtgactaa aaacaataca aaattagctg tagttgatat cggttatggc
    10441 gatggaattt taagaacacg tgctaaacat gaagcactta tcaatggtaa acgctacccg
    10501 atacgtgcat taatgatgag ccatatgttt gttgaagtag atggcaatgt acatgcacaa
    10561 gatgaagtta ttctttataa taatgatatc cgcatcgatg aatatacctt taaaggtgtt
    10621 ggtgcaaatt ctgaacaatt aagtgctatg aatcatgatt ctttaaaaaa ggagtacatt
    10681 tcaaatgact gttaaatata atcaaaatgg cgaattaaca atggatggta ttagtttaaa
    10741 aacgattgcg caaagctttg gtacacctac cattgtttat gatgaactac aaattagaga
    10801 acagatgcgc cgttaccatc gcgcatttaa agatagtgga ttaaaataca atatttcata
    10861 cgcctcaaag gcatttactt gcattcaaat ggtcaaactt gtagctgagg aagatttaca
    10921 gttagatgtt gtttctgaag gtgaattata tacagcttta gaagcaggtt ttgaaccgag
    10981 tcgcatccat ttccatggta acaataaaac gaaacatgaa attaggtatg ctttagaaaa
    11041 taatatcggt tattttgtta tagattcatt agaagaaatt gaattaatag accgctatgc
    11101 taatgatacg gtgcaagttg tattacgagt taatccaggt gttgaagcac atacacacga
    11161 atttattcaa actgggcaag aagatagtaa gtttggatta tcaattcaat atggcttagc
    11221 taaaaaagca attgacaaag tccaacaatc taaacactta aaattaaaag gtgtacattg
    11281 tcatattggt tcacagattg aaggtacaga agcatttatt gaaactgcta aaattgtttt
    11341 acgttggctt aaagagcaag gcattcaagt tgaattatta aaccttggtg gtggctttgg
    11401 tattaaatat gttgaaggtg acgaaagttt ccctatcgaa agtggtatta aagatattac
    11461 agacgcaata aaatccgaaa ttaaagttct aggtatagat gcaccagaaa taggtattga
    11521 accgggacga tcaattgtag gtgaagctgg cgttacttta tatgaagttg gaaccattaa
    11581 agaaattcca gagattaata aatatgtttc aatcgatggc ggtatgagtg atcatatcag
    11641 aactgcactt tatgacgcaa agtatcaagc attgcttgtt aatagaaatg aagaagcaga
    11701 tgacagtgta actatagctg gaaaattatg tgagtctggt gatatcatta ttaaagacgc
    11761 taaattacct tcatcagtca aacgtggaga ctatcttgct atattatcaa ctggtgcata
    11821 tcattactct atggcatcca attacaatca aatgcaaaag ccttctgtgt ttttcttaaa
    11881 agatggcaaa gcacgtgaag ttataaagcg acaatcgtta agacaactca ttattaatga
    11941 tacaaaataa aaataaacaa agtaatcccc gaagcacaga aaattatact gtgactcggg
    12001 gaatttttat gcattataga tgactgtaga aaacatgctt ggacatatac tctaaaaaag
    12061 gaagaacagc acatttttag ttagaaagta tttatttctt tttgtcatta atcctttata
    12121 tttagttagc cctattcaac taatttaaag catcatatac ataccacact ttaaacaagc
    12181 atcatgaaat tagattttat gctttacttg ctttttcaaa taatataaaa cgataagaat
    12241 taacaacaaa tataatgtat aaaacagaat ttcatcaaat ccaaaatctt tacttgtaat
    12301 taatacatca atatcttcta attccatcat ctctgcttta atat
//
LOCUS       DAMZKC010000077        12085 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.077, whole genome shotgun sequence.
ACCESSION   DAMZKC010000077 DAMZKC010000000
VERSION     DAMZKC010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12085
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.077"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(1..110)
                     /locus_tag="POB55_001531"
                     /pseudo
     CDS             complement(1..110)
                     /locus_tag="POB55_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498838.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
     gene            261..1259
                     /locus_tag="POB55_001532"
     CDS             261..1259
                     /locus_tag="POB55_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDI6500496.1"
                     /translation="MKKVSIKDVAREAGVSVTTVSHILNHNDSRFSATTIKNVHAVSE
                     RLGYAPNKHAKQLRGSKIQTIGVILPSLTNPFFSALMQSIHDHKPSDVDLCFLTSTAT
                     DLYDNIKHLIDRGIDGLIIAQYISSPDALNNYLKKHHVPYVVLDQNDHQGYTDFVRTN
                     EYQGGQLAAQHLIELGHNNMMIVAPYDMMANMSTRVAGFVDTLRANQLPEPQIVHTEL
                     SKRGGLTIVDDIMVQSATAIFAINDELAIGILRGLIEHGISIPKDISLIGYDDIDYAA
                     YVSPPLTTVAQPITDIGKTSLTLLLQRLQHLDKSIDMIELPTTLKIRATTGYHLSN"
     gene            1491..2372
                     /gene="rbsU"
                     /locus_tag="POB55_001533"
     CDS             1491..2372
                     /gene="rbsU"
                     /locus_tag="POB55_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose transporter RbsU"
                     /protein_id="HDI6500497.1"
                     /translation="MSIVALLIGLGPLIGWGFFPTVASKFGGKPVHQIIGATVGTLIF
                     AIILAVVTSSGFPTGTNLLFALLSGAGWGFGQIITFKAFELVGSSRAMPVTTAFQLLG
                     ASLWGVFALGNWPGIGHKIIGFTALVVILIGARMTVWSERKEASNAKNLRRAVVLLLI
                     GEFGYWLYSAAPQATSIDGLTAFLPQAMGMVIVAVIYGFMNMKAENPFRNKITWLQII
                     SGFFFAFGALTYLISAQPNMNGLATGFILSQTSVVLATLTGIYFLKQHKTSKEMVITI
                     IGLVLILVAASVTVFIK"
     gene            2387..2791
                     /gene="rbsD"
                     /locus_tag="POB55_001534"
     CDS             2387..2791
                     /gene="rbsD"
                     /locus_tag="POB55_001534"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HDI6500498.1"
                     /translation="MKKSAVLNEHISKAIATIGHFDLLTINDAGMPIPNDHRRIDLAV
                     TKNLPRFIDVLATVLEEMEIQKIYLAEEIKEHNPTQLQQIKQLISSEIEIIFIPHEEM
                     KSNLAHPLNKGNIRTGETTPYSNIALESNVTF"
     gene            2819..3733
                     /gene="rbsK"
                     /locus_tag="POB55_001535"
     CDS             2819..3733
                     /gene="rbsK"
                     /locus_tag="POB55_001535"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDI6500499.1"
                     /translation="MTNKVVILGSTNVDQFLTVERYAQPGETLHVEEAQKAFGGGKGA
                     NQAIATARMQADTTFITKIGTDGVADFILEDFKAAHIDTSYIIKSTEAKTGQAFITVN
                     AEGQNTIYVYGGANMTMTPEDVINAKDAIINADFVVAQLEVPIPAIISAFEIAKAHGV
                     TTVLNPAPAKALPNELLSLIDIIVPNETEAELLSGIKVTNEQSMKDNANYFLSLGIKT
                     VLITLGKQGTYFATKNQSQHIEAYKVNAIDTTAAGDTFIGAFVSRLNKSQDNLADAID
                     FGNKASSLTVQKHGAQASIPLLEEVNQV"
     gene            complement(3831..3965)
                     /locus_tag="POB55_001536"
     CDS             complement(3831..3965)
                     /locus_tag="POB55_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500500.1"
                     /translation="MIIIIINIAYLYAIIWKLKRLQKIVTSFITKHESTSIGLVENYF
                     "
     gene            3984..4745
                     /locus_tag="POB55_001537"
     CDS             3984..4745
                     /locus_tag="POB55_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006191654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDI6500501.1"
                     /translation="MSKEAGHTFLAKLGKTRLRPGGKEATDWLIQQGAFSQDKQVLEV
                     ACNMCTTSIYLAHTYGCHIQGVDINKKALEKAQENISAAGLESYIQVQQANAVKLPFD
                     DNQFDIVLNEAMLTMLPITIKEKALREYYRVLKPGGILLTHDIVIVNESHATHVVKSL
                     SAAINVNVSPQTKLGWLDLYNQAGFNHVHYHTGPMSLMTPKGLIYDEGIVGTIKIINN
                     ALKKENRPMFCKMFKTMTKLRKDMNYITFVAKKED"
     gene            complement(5126..6562)
                     /locus_tag="POB55_001538"
     CDS             complement(5126..6562)
                     /locus_tag="POB55_001538"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDI6500502.1"
                     /translation="MTKLPRNFMWGGALAANQFEGGYDKGGKGLSVIDVMTSGAHGKA
                     RQITKSIDQNHYYPNHEGIDFYHRYKEDIALFNEMGLKCLRTSIAWTRIFPNGDEDVP
                     NEEGLAFYDRIFDELIAQDIEPVVTLSHFEMPLHLAKHYGGFRNREVVDYFVHFARVV
                     FERYKDKVTYWMTFNEINNQMDTSNPIFLWTNSGVALTENDNPEEVLYQVAHHELLAS
                     ALAVRLGKEINPKFKIGTMISHVPIYPYSCHPKDMMEAQIANRLRFFFPDVQVRGYYP
                     SYAKKMLARKGYDVGWQEGDDSILQQGTVDYIGFSYYMSTAVKHDVDTTVENNIVNGG
                     LNHSVENPHIATSDWGWAIDPDGLRYTLNVLYDRYQLPLFIVENGFGAVDEVVDGHIH
                     DDYRIEYLKAHITAAIEAVDQDGVDLIGYTPWGIIDIVSFTTGEMKKRYGLIYVDRDN
                     DGHGTMERLKKDSFYWYQQVIASNGDKL"
     gene            complement(6578..7369)
                     /locus_tag="POB55_001539"
     CDS             complement(6578..7369)
                     /locus_tag="POB55_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDI6500503.1"
                     /translation="MSNKYKEQAQDILTAVGGVENIVDATYDTKCITIHMQHTIPSTA
                     NEVKQIVDVTSVAENDAQLVIKLNGNVDEVYQQLQRLIKNANVEESENTDNINSQDTS
                     YIPQVKVTTPILVKAPIAGRRILLKEVRDSIFREKMVGEGLAIKAHEESKVIAPFNGL
                     ISMIVPTKHAVRIQSEDGVDIVIHIGVNTVDLEGKGFKCFVKQNDRVEAGQTLLQFDQ
                     QYIQQQGYNADVIVVISNSADLGKVELTMNEIITTEDVIFKIFKN"
     gene            7518..8222
                     /locus_tag="POB55_001540"
     CDS             7518..8222
                     /locus_tag="POB55_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDI6500504.1"
                     /translation="MLKYEHIAKQLNAFIHQSKFKPGDKLPSVTQLKERYQVSKSTII
                     KALGLLEQDGLIYQAQGSGIYVRNIADANRINVFKTNGFSKSLGEHRMTSKVLVFKEM
                     ATPPKSVQDELQLNADDTVYYLERLRFVDDDVLCIEYSYYHKEIVKYLNDDIAKGSIF
                     DYLESNMKLRIGFSDIFFNVDKLTSSEASLLQLSTGEPCLRYHQTFYTMTGKPFDSSD
                     IVFHYRHAQFYIPSKK"
     gene            complement(8330..9031)
                     /gene="lrgB"
                     /locus_tag="POB55_001541"
     CDS             complement(8330..9031)
                     /gene="lrgB"
                     /locus_tag="POB55_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiholin-like protein LrgB"
                     /protein_id="HDI6500505.1"
                     /translation="MINHLALNTPYFGILLSVIPFFLATILFEKTNRFFLFAPLFVSM
                     VFGVAFLYLTGIPYKTYKIGGDIIYFFLEPATICFAIPLYKKREVLVKHWHRIIGGIG
                     IGTVVALLIILTFAKLAQFANDVILSMLPQAATTAIALPVSAGIGGIKELTSLAVILN
                     GVIIYALGNKFLKLFRITNPIARGLALGTSGHTLGVAPAKELGPVEESMASIALVLVG
                     VVVVAVVPVFVAIFF"
     gene            complement(9024..9467)
                     /gene="lrgA"
                     /locus_tag="POB55_001542"
     CDS             complement(9024..9467)
                     /gene="lrgA"
                     /locus_tag="POB55_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiholin-like murein hydrolase modulator LrgA"
                     /protein_id="HDI6500506.1"
                     /translation="MVVKQQKDASKPAHFFHQVIVIALVLFVSKIIESFMPIPMPGSV
                     IGLVLLFVLLCTGAVKLGEVEKVGTTLTNNIGLLFVPAGISVVNSLGVISQAPFLIIG
                     LIIVSTILLLICTGYVTQIIMKVTSRSKGDKVTKKIKIEEAQAHD"
     gene            complement(9580..10320)
                     /locus_tag="POB55_001543"
     CDS             complement(9580..10320)
                     /locus_tag="POB55_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor LytR"
                     /protein_id="HDI6500507.1"
                     /translation="MKALIIDDEPLARNELTYLLNEIGGFEEINEAENVKETLEALLI
                     NQYDIIFLDVNLMDENGIELGAKIQKMKEPPAIIFATAHDQYAVQAFELNATDYILKP
                     FGQKRIEQAVNKVRATKAKDDNSASAIANDMSANFDQSLPVEIDDKIHMLKQQNIIGI
                     GTHNGITTIHTTNHKYETTEPLNRYEKRLNPAYFIRIHRSYIINTKHIKEVQQWFNYT
                     YMVILTNGVKMQVGRSFMKDFKASIGLL"
     gene            complement(10323..12077)
                     /locus_tag="POB55_001544"
     CDS             complement(10323..12077)
                     /locus_tag="POB55_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDI6500508.1"
                     /translation="MLSLTMLLLERVGLIIILAYVLMNIPYFKNLMNRRRTWKARWQL
                     CIIFSLFALMSNLTGIVIDHQHSLSGSVYFRLDDDVSLANTRVLTIGVAGLVGGPFVG
                     LFVGVISGIFRVYMGGADAQVYLISSIFIGIIAGYFGLQAQRRKRYPSIAKSAMIGIV
                     MEMIQMLSILTFSHDKAYAVDLISLIALPMIIVNSVGTAIFMSIIISTLKQEEQMKAV
                     QTHDVLQLMNQTLPYFKEGLNRESAQQIAMIIKNLMKVSAVAITSKNEILSHVGAGSD
                     HHIPTNEILTSLSKDVLKSGKLKEVHTKEEIGCSHPNCPLRAAIVIPLEMHGSIVGTL
                     KMYFTNPNDLTFVERQLAEGLANIFSSQIELGEAETQSKLLKDAEIKSLQAQVSPHFF
                     FNSINTISALVRINSEKARELLLELSYFFRANLQGSKQHTITLDKELSQVRAYLSLEQ
                     ARYPGRFNININVEDKYRDVLVPPFLIQILVENAIKHAFTNRKQGNDIDVSVIKETAT
                     HVRIIVQDNGQGISKDKMHLLGETSVESESGTGSALENLNLRLKGLFGKSAALQFEST
                     SSGTTFWCVLPYERQEEE"
BASE COUNT         3927 a         2285 c         1930 g         3943 t
ORIGIN      
        1 tatttaaaat aatcactttt gtatttttca tcacaataaa cattttaaaa ttcttgttat
       61 cataatcatt aaaagttatt taaccttaat aatatattct ctcgcctcaa ccttaatcgc
      121 atactatagg cgtctgtttg tagacaataa aagtcattca cgtcttcata tgtcatcaaa
      181 tgtttatcat gatatgatga atataataat cgggtatata actgtatgat taattacaca
      241 ataaagagaa ggaatataat atgaaaaaag tgtcaattaa agatgttgct agagaagctg
      301 gtgtatcagt tacaactgtg tcacatattt taaatcataa tgatagtcgt ttttccgcaa
      361 caacgataaa aaacgtacat gctgtttcag aacgtttagg ctatgcccct aataaacatg
      421 caaaacaatt gcgcggcagt aaaattcaaa ctattggcgt cattttgcct agcttaacaa
      481 atccgttttt ctcagcactg atgcaaagta ttcatgacca taaaccatct gatgttgatt
      541 tatgcttttt aacatctaca gcaactgatt tgtatgacaa tattaaacat ttaattgatc
      601 gaggtattga tggattaatt atcgcacaat acatttcatc cccggacgcc ctaaataatt
      661 atctaaagaa acatcatgta ccttatgtcg tactggatca aaatgaccat caaggctata
      721 cagattttgt tcggacaaat gaatatcaag gcggacaact tgcagcacaa catttaatag
      781 aactcggtca caacaatatg atgattgttg caccatatga catgatggcg aatatgtcga
      841 ctcgtgtcgc tggatttgtt gatactttgc gcgcgaatca attgccagaa ccacaaatcg
      901 tccatactga attatctaag cgcggtgggc taaccattgt tgatgacatc atggttcaat
      961 ctgccactgc aatcttcgct attaacgatg aactcgctat tggcatttta cgaggactaa
     1021 ttgaacatgg catcagtatt ccgaaagata tctcattaat aggttatgac gacattgatt
     1081 atgcagcgta cgtctcgcca cctttaacta ctgtggcaca acctataact gatattggca
     1141 aaacatcttt aaccttatta cttcaacgat tacagcactt agataaatcc attgatatga
     1201 ttgaattacc aacgacttta aaaattcgtg caacaactgg ctatcatctt tcaaactaac
     1261 tacgtatctt ccgaaatata ctcatcattg ttatgccctt agcgttgctt taatgctgag
     1321 ggtttttaat cataattatt tcactaagaa attaaaataa taatgtatga atttttaaat
     1381 atgatttaaa cgttttcatt ttttatgaaa acgcatgcat tttacaaata aaaatggtac
     1441 gatggcactg gtaaaacgtt ttactaaaaa caaatcgtga ggtgtataac atgagcattg
     1501 ttgcattact tatcgggtta ggccccttaa ttggctgggg cttcttccca acagtcgctt
     1561 caaagtttgg tggtaaacct gtacatcaaa ttatcggtgc tactgtaggt acgttaatct
     1621 tcgctattat attagccgta gtcacatcaa gtggcttccc tactggaacc aatttgctat
     1681 tcgccttatt atcaggtgca ggatggggat tcggacaaat cattacattt aaagcgttcg
     1741 aattagtcgg ctcatctcgt gccatgccag tcacaacagc attccaatta ttaggcgcat
     1801 ctttatgggg tgtctttgca ttaggaaatt ggccaggcat tggtcataaa atcattggat
     1861 ttacagcttt agtcgttatt ctaattggag cgcgtatgac agtttggagt gaacgcaaag
     1921 aagcaagtaa cgccaaaaat ttacgtcgtg cagtggtact tctgttaatt ggtgaatttg
     1981 gatactggtt atattcagct gcaccgcaag caacttctat tgatggccta actgcctttt
     2041 tacctcaagc aatgggtatg gtaattgttg cagtcattta tggctttatg aatatgaaag
     2101 cagagaatcc attccgtaat aaaattacgt ggttacaaat tatttcaggt ttcttctttg
     2161 catttggtgc tttaacatat cttatttcag cacaacctaa tatgaatggt ttagcaactg
     2221 gatttattct ttctcaaaca tccgttgtgc ttgctacatt aactggtatt tatttcttaa
     2281 aacaacataa aacgtcaaaa gaaatggtta ttacaatcat cggcttagta ctcattttag
     2341 tagccgcttc tgttacagta tttataaaat aaggagtgta gatgtcatga aaaaatcagc
     2401 tgttttaaat gaacatattt caaaagcaat cgcgacaatt ggtcattttg atttattaac
     2461 gattaatgac gctggcatgc caattccaaa tgatcatcgt cgtatcgacc tagctgtaac
     2521 taaaaactta ccacgcttta ttgatgtctt agctacagtg ttagaagaaa tggaaatcca
     2581 aaaaatatac ttagcagaag aaataaaaga acataaccct acacaattgc aacaaattaa
     2641 acaattgatt tcatcggaaa tcgaaatcat tttcattcct cacgaagaaa tgaaaagtaa
     2701 cttagctcac ccattaaata aaggtaatat tcgtactggt gaaacaacgc cctactctaa
     2761 tattgcatta gaatcgaatg ttacttttta aaagttataa cttgaaagga gcatacacat
     2821 gaccaacaaa gttgttattt taggttcaac gaatgtcgat caatttttaa cagttgaaag
     2881 atatgcacaa ccaggcgaaa cattacatgt tgaagaagca caaaaagcat tcggcggagg
     2941 taaaggtgcc aaccaggcta ttgccactgc acgtatgcaa gcagacacaa catttattac
     3001 taaaattggc actgatggcg ttgctgattt catcttagaa gattttaaag cggctcatat
     3061 tgatacatca tatattatca aatcaactga agcaaaaaca ggccaagcct ttatcactgt
     3121 gaatgcagaa ggacaaaaca ccatctatgt ttatggtggt gcgaatatga cgatgacacc
     3181 tgaagatgtt attaacgcaa aagacgctat aatcaatgca gactttgtcg ttgcacaatt
     3241 agaagtaccc atcccggcta ttatatctgc atttgaaatt gccaaggcac atggtgtgac
     3301 gacagtatta aatcctgcac cagcgaaagc attacctaat gaattattat cattaatcga
     3361 tattattgtg ccaaacgaaa cagaagccga attgttatct gggattaaag taactaatga
     3421 acaatctatg aaagacaatg ccaattactt tttatcttta ggcattaaga ctgttttgat
     3481 tacgctaggt aagcaaggta catattttgc tactaaaaat caaagccaac acatcgaagc
     3541 ttataaagta aatgcgattg atacaactgc tgcaggcgac acatttattg gtgcatttgt
     3601 cagtcgctta aacaagtcgc aagataactt agcagatgct attgattttg gtaataaagc
     3661 gagctcactc actgtacaaa aacacggcgc gcaagcatct attcctctac tagaagaagt
     3721 aaatcaagtt taaatgaagc aaacacagct atgatatgaa ggtttagcat ataacatgca
     3781 acattcgtat atcatggctg tgctttttta tctttataaa acatcatcta ttagaaataa
     3841 ttttccacta aacctatgct tgttgactca tgtttagtta taaatgaagt gacaattttt
     3901 tgtaatcttt ttaacttcca aattattgca tataaatatg ctatattaat gataataatt
     3961 atcaattaaa aggaggttat gctatgtcta aagaagctgg tcatacattt ttagctaaat
     4021 taggaaaaac tcgtctacgc cccggtggta aagaagcaac agattggtta atacaacaag
     4081 gggcattttc acaagataaa caagtgttag aagtggcatg taatatgtgc acaacatcta
     4141 tttatctagc tcatacatat ggctgtcaca ttcaaggcgt tgatataaat aagaaagcat
     4201 tagaaaaagc acaggaaaat atttcagcag caggtcttga atcatatatt caagttcaac
     4261 aagcgaatgc tgttaaattg ccctttgatg acaatcaatt cgatatcgtt ttaaatgaag
     4321 caatgttaac aatgttaccc atcaccataa aggaaaaagc attacgcgag tactaccgag
     4381 tcttaaagcc tgggggtatc ttgttaacac atgatattgt catcgttaat gaatcacatg
     4441 ccacacatgt agttaaatca ttatctgcag caattaatgt caatgtctca ccgcagacga
     4501 aacttggctg gttagattta tataatcaag ctggttttaa tcatgtgcat tatcatactg
     4561 gtccaatgag tttaatgaca ccaaaaggtt taatttatga cgaaggtatt gttggaacta
     4621 taaagattat caacaatgct ttaaaaaaag aaaatcgacc aatgttttgt aaaatgttta
     4681 aaacgatgac taaattgcgt aaagatatga attatattac ttttgtcgct aaaaaagagg
     4741 actaaatata atgccactaa ccgtactttg tatctatgtt tgactgctac tttaatttct
     4801 ttgtgacact aatcatctac ttaacaatat cgttatcgtt gattagtaag tcatcaattt
     4861 tggttaaaga ctttcataaa cactcaaaca ttaacactat acatacttag ttagtggcat
     4921 tattttttta aaaattttaa catcacggga ttggaacaga aatgatattt tcgcaaaatt
     4981 tattcctgtc cttccccaac ttgcacatta ttgtatgctg actttccgcc agcttctatg
     5041 ttggggcccg ccaatataac actgtaggtc ctactatatt aatttatgtc tcgggctcta
     5101 accctaaaat acttataata tacctttata atttatctcc atttgatgct atcacttgtt
     5161 gataccaata gaacgaatct tttttcaagc gttccatcgt gccatgacca tcattatctc
     5221 gatcaacata tattaaacca tagcgtttct tcatttcacc ggttgtaaat gaaacaatat
     5281 caatgattcc ccacggtgta taaccgatta aatctacacc atcttgatca actgcttcta
     5341 tcgctgctgt aatatgtgct tttaaatatt caatgcgata atcatcatga atatgtccat
     5401 ctaccacttc atcaactgca ccaaaaccat tttccacaat aaaaagtggt aactgataac
     5461 gatcatataa cacattcaat gtatatctta agccatctgg atcaatcgcc caaccccaat
     5521 cactcgttgc gatatgcgga ttctccacag aatgattcaa accaccgttg acgatgttgt
     5581 tttcaactgt agtatcaaca tcatgtttta cagccgtaga catgtaataa ctaaagccaa
     5641 tataatcaac cgtgccctgc tgtaaaatac tgtcgtcccc ttcttgccat ccaacatcat
     5701 atccttttcg tgccaacatt tttttagcat agcttggata ataacctctc acttggacat
     5761 ccgggaaaaa gaaacgtaag cgattcgcaa tttgtgcttc catcatatct ttcggatgac
     5821 acgaatatgg ataaatgggt acatgtgaaa tcattgttcc aatcttaaac ttcggattaa
     5881 tctctttacc aagacgaact gctaaagcac tggctaaaag ttcatgatgt gctacttgat
     5941 acaagacttc ttcaggatta tcattttctg tcaatgctac cccagaattc gtccataaaa
     6001 agataggatt tgatgtgtcc atctgattat taatttcatt aaacgtcatc caatatgtca
     6061 ctttatcttt atatctttca aatacaacac gcgcaaaatg cacaaaataa tcgacaactt
     6121 ctctatttct aaatccacca taatgtttcg ctaaatgaag tggcatctca aaatgtgata
     6181 acgtcacaac aggttcaata tcttgtgcaa ttaattcatc aaagatacga tcataaaagg
     6241 cgagtccttc ttcgtttggc acatcttcat ccccattcgg aaagatacgt gtccacgcaa
     6301 tcgacgtacg taaacatttc aatcccattt cattaaacaa ggcaatatct tccttataac
     6361 gatgataaaa atcaatacct tcatgatttg gataatagtg attttgatct atagactttg
     6421 taatctgacg tgctttgcca tgtgcaccac tcgtcataac atcaattaca cttaaccctt
     6481 tacccccttt atcatatcca ccttcaaatt gatttgcggc aagagcgcca ccccacataa
     6541 aatttcgcgg taatttggtc attattacaa cacactccta gtttttaaat attttaaaaa
     6601 taacatcttc agtcgtaatg atttcattca ttgtcagttc tacttttcct aaatcggcag
     6661 agttgctaat aacgacaata acgtcagcat tgtagccttg ttgttgtata tattgctggt
     6721 cgaattgtaa caacgtttgc cctgcttcaa cacgatcatt ttgctttaca aagcacttga
     6781 accctttacc ttccaagtca actgtattca cgccaatatg aatgactatg tccacaccgt
     6841 cttctgattg aatacgaact gcatgcttag ttggtacaat catagatatt aaaccattga
     6901 acggtgcgat tactttggat tcttcatgag ctttgattgc caagccttcg cctaccattt
     6961 tctctctaaa aattgaatct cttacttctt taagtaaaat acgacgacca gcgattggtg
     7021 ctttcactaa aattggtgtt gttactttta cttgaggtat ataacttgta tcttggctat
     7081 taatattgtc agtattctca ctctcttcga cattagcatt cttaattaat cgctgtaatt
     7141 gctgatacac ttcatcgaca tttccattta attttatgac taactgcgca tcattttctg
     7201 ctacagatgt cacatcaact atttgtttca cttcatttgc tgtagaagga attgtatgtt
     7261 gcatatgaat tgtaatgcac ttcgtgtcat acgttgcatc aacaatgttt tcgacaccac
     7321 ctacagctgt aagaatgtct tgggcttgtt ctttatattt attgctcata aatattaccc
     7381 cctcaacttg atagtagaat gaaaatgtat ttacttacct ccaattataa ttgtaccggt
     7441 tcaatttgta aacgccgata caattataat attttgtgct ataataatta cagacaaagt
     7501 gaaacgagga cagaatattg ttaaagtatg aacatattgc taagcaactt aatgcgttta
     7561 tacatcaatc taaattcaaa cccggtgata aattgccaag cgtgacgcaa ttaaaagaac
     7621 gttatcaagt aagtaagagt actatcatta aagcattagg cttattggaa caagatggtt
     7681 tgatctatca agcacaaggc agtggtattt atgtgagaaa tattgcagat gccaatcgta
     7741 tcaacgtctt taagactaat ggtttctcta aaagtttagg tgaacatcga atgacaagta
     7801 aggtacttgt ttttaaggag atggcaacgc cacctaaatc tgtacaagat gaactccaat
     7861 taaatgcaga tgataccgtc tactatttag agcgattaag attcgtggac gatgatgttt
     7921 tatgtatcga atattcttat tatcataaag aaatcgtgaa atatttaaat gatgatattg
     7981 ctaagggctc tatcttcgat tatttagaat caaacatgaa acttcgtatt ggtttttcag
     8041 atattttctt taatgtagat aaactcactt caagtgaagc ttcattacta caattgtcta
     8101 caggtgaacc atgtttacgt taccaccaga ctttttatac aatgactggc aaaccctttg
     8161 attcgtctga catcgtattt cattatcgtc atgcacagtt ttatattcct agtaaaaagt
     8221 aataaataca taaaaacgtc tatatcccag ttataaactg gagtatagac gtttttttac
     8281 gataataaca atggctcaaa ttgctattat cttgcttagg tttttcgttt tagaagaata
     8341 ttgctacaaa gacaggcaca actgctacaa caactacacc aactaacact aaagctatac
     8401 ttgccattga ttcttctaca ggtcctaatt ctttggctgg tgctacacct aatgtgtgac
     8461 cacttgttcc aagtgctaat cctcgggcaa tagggttagt aattcggaaa agcttcaaga
     8521 atttattacc tagggcataa ataatgacac catttaaaat aactgctaat gatgttaatt
     8581 cttttatacc accgatacca gctgatactg gtaacgcaat cgctgtagtt gctgcttgag
     8641 gtaacattga taaaataaca tcattggcaa attgtgctaa cttcgcaaaa gttaaaataa
     8701 ttaataacgc tacaactgta ccgataccaa tacctccgat gatacgatgc caatgtttaa
     8761 caagcacttc acgcttttta tataacggaa tcgcaaaaca gattgttgcc ggttctaaga
     8821 agaagtaaat aatgtctcca cctattttgt aagtcttata cggaatgcct gttaaataga
     8881 ggaaggccac accaaatacc atactgacaa atagcggtgc gaataagaag aaacgattag
     8941 ttttttcaaa taatatggtc gctaagaaaa atggtataac ggataacagt attccgaagt
     9001 aaggtgtatt tagtgctaag tggttaatca tgagcttgtg cctcctctat tttgatcttt
     9061 tttgtgactt tgtcaccttt agatctcgaa gtaactttca taataatttg tgtgacatag
     9121 ccagtacaaa taagtaatag tattgttgag acgattatta gtccaatgat taaaaatggt
     9181 gcttggctaa tgacacctaa agagttaaca actgagatac cggctggtac gaagagtaag
     9241 ccaatgttat ttgttagtgt cgttcctact ttttcgactt cgcctaactt aacagcacca
     9301 gtacataata atacaaataa taatactaaa ccgattactg atccaggcat aggaattggc
     9361 ataaatgatt caattatttt cgatacaaag agtactaaag caattacaat gacttggtga
     9421 aaaaagtgtg ctggttttga tgcgtctttt tgttgtttca cgaccattgc ctcctacgtt
     9481 tgatttaact aaagtataga tggctcactt cgatttgcgt aaattttagt ccgaaataca
     9541 aaatatcata ggtaaaatgc ataaaaaaaa ggattactgt taaagtaacc ctatcgacgc
     9601 tttaaaatct ttcataaatg aacgtccaac ttgcatcttg acaccatttg tcaatattac
     9661 catataagtg tagttaaacc attgttgcac ttctttaatg tgtttcgtat taataatata
     9721 tgaacgatga atacgtatga aataagcggg attcaatcgt ttttcataac gattcaatgg
     9781 ctctgttgtt tcgtatttat gattcgttgt atgtatggtt gtaataccat tatgtgtgcc
     9841 aatcccaata atattttgtt gctttaacat gtgaatttta tcgtctattt caacaggtaa
     9901 actttgatca aaattcgccg acatatcatt cgcaattgca cttgcgctat tatcatcttt
     9961 ggctttagtc gcacgcactt tattgactgc ttgttcaata cgtttttgac caaacggttt
    10021 caaaatatag tctgtcgcat ttaattcaaa tgcctgtact gcgtattggt catgtgcagt
    10081 tgcaaaaata atcgcaggtg gctctttcat cttttgaatc ttagctccta attcgatccc
    10141 attttcatcc attaaattga catctaaaaa tataatgtca tattgattga tcagtagtgc
    10201 ttccaatgtt tcttttacat tttctgcctc attaatttct tcaaaaccac caatttcatt
    10261 taataaatat gttaattcat tacgtgctaa tggctcatca tctatgatta atgctttcat
    10321 atttattcct cctcttgtct ttcataagga agtacacacc aaaaagtggt accgctcgat
    10381 gtcgattcaa attgtaatgc tgcggatttt ccaaataatc cttttaggcg taagtttaaa
    10441 ttttctaaag cactaccagt tccagactct gattctacag atgtttctcc caacaaatgc
    10501 attttatctt tagaaatacc ctgaccatta tcttgtacaa taatacgtac atgtgttgca
    10561 gtttctttaa tcactgacac gtcaatatcg ttaccttgct ttcgattcgt aaacgcatgt
    10621 ttgatggcat tttcaactaa aatttgaatt aaaaatggtg gtacaagcac atcgcgatat
    10681 ttgtcttcaa cattaatatt gatattaaat cttcctggat aacgtgcttg ttcgagtgat
    10741 aagtatgcac gcacttgact taactcttta tctaaagtaa tcgtatgttg cttagagcct
    10801 tgtaaattcg ctctgaaaaa ataactcaat tctaatagta actctcgtgc cttttcgcta
    10861 tttattctaa ctaaagctga aatcgtgtta attgaattga agaaaaaatg tggactcact
    10921 tgtgcctgta atgacttaat ctcagcatct ttcaataact tactttgcgt ttcggcttca
    10981 ccaagttcaa tttggctact aaaaatattt gccaatcctt ctgcaagttg acgttccaca
    11041 aaagttaaat cattagggtt tgtaaaatac atcttcaatg taccgacgat agaaccatgc
    11101 atctcaagtg gtatcacgat agctgctcta agcgggcaat tcggatgact acaaccaatc
    11161 tcttctttag tatgaacttc tttcaacttt cctgatttca atacatcttt agacagactt
    11221 gttaatattt catttgttgg tatgtgatga tcactacctg cacctacatg cgataagatt
    11281 tcatttttgc ttgtaattgc tacggcagat actttcatta aatttttaat aatcatcgca
    11341 atttgctgtg ccgattctct attcaatcct tctttaaaat acggcaatgt ctggttcatc
    11401 aattgcagta catcatgtgt ttgaacagcc ttcatttgct cctcttgctt taatgttgaa
    11461 atgataatag acataaaaat cgccgtaccg acgctattaa caataatcat tggtagtgca
    11521 attaatgata tgaggtcaac cgcatatgct ttgtcgtggg aaaatgttaa aatgctcaac
    11581 atttgaatca tttccataac aattccaatc atggcacttt tcgcaatact cgggtaacgc
    11641 ttgcgtcttt gagcttgtaa gccaaaataa ccagcaatta taccgataaa tatagatgag
    11701 ataagataaa cttgtgcatc cgccccaccc atatacactc tgaaaatacc tgaaataacg
    11761 ccaacaaata gacctacaaa agggccacca actaatcctg cgacacctat cgttaataca
    11821 cgtgtgttag ctaaagatac atcatcatct aaacggaagt acacacttcc tgacaaacta
    11881 tgttgatgat cgatgacgat accagttaaa ttagacatta aggcaaacaa actgaaaata
    11941 atacataatt gccaacgtgc tttccatgta cgtcgacgat tcattaagtt tttaaaatat
    12001 ggaatattca tcaacacata ggccaaaata ataattaaac ctacacgctc aagtaataac
    12061 attgttagcg atagcacgat agcac
//
LOCUS       DAMZKC010000078        11904 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.078, whole genome shotgun sequence.
ACCESSION   DAMZKC010000078 DAMZKC010000000
VERSION     DAMZKC010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11904
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.078"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            229..993
                     /locus_tag="POB55_001545"
     CDS             229..993
                     /locus_tag="POB55_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exotoxin"
                     /protein_id="HDI6500509.1"
                     /translation="MNNCFGEFEVKYLTGFILILLLEEILTNSASAIEYSDLHHKSKF
                     DSKRLSNAKMSFINPTQLENKNTNDRLLKHDLLFHDMFVNDDWKKDFKVEFENEALSK
                     KFINKDIDIFAGNYGYGCHGGATNKTQCSYGGVTLSDNNKYDDYKNIPCNLWIDGHQA
                     EIELTAVKTKKKIVTIQELDVQLRNYLNEKYKLYEQGGDIVKGYVKYHNDDEQNIEYN
                     FYNLNGEYGYEVLKMYADNKTINSDKLHLDIYLFKS"
     gene            complement(1195..1464)
                     /locus_tag="POB55_001546"
     CDS             complement(1195..1464)
                     /locus_tag="POB55_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500510.1"
                     /translation="MAENNQNSLVTKIATYGSFIAIASFVILFISIFLKFSFNIEFTA
                     TIMNICRYTLILGFILMSLPDVVDKNIKKIIFDAFIIIVFIFFLL"
     gene            1778..2464
                     /gene="mtnN"
                     /locus_tag="POB55_001547"
     CDS             1778..2464
                     /gene="mtnN"
                     /locus_tag="POB55_001547"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HDI6500511.1"
                     /translation="MIGIIGAMEEEVTILKNKLTQLSEISVAHVKFYTGILKDREVVI
                     TQSGIGKVNAAISTTLLINKFKPDVIINTGSAGALDESLNVGDVLISDDVKYHDADAT
                     AFGYEYGQIPQMPVAFQSSKPLIEKVSQVVQQQQLTAKVGLIVSGDSFIGSVEQRQKI
                     KKAFPNAMAVEMEATAIAQTCYQFNVPFVVVRAVSDLANGEAEMSFEAFLEKAAVSSS
                     QTVEALVSQL"
     gene            2484..3011
                     /locus_tag="POB55_001548"
     CDS             2484..3011
                     /locus_tag="POB55_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HDI6500512.1"
                     /translation="MGLVRKFFMPNSYVQSIFQIDLDKLVDKGVKGIITDLDNTLVGW
                     DVKEPTERVKAWFKEANEKGITITIVSNNNESRVASFSQHLDIDFIFKARKPMGKAFD
                     KAITKMNIRPDQTVVIGDQMLTDVFGGNRRGLYTIMVVPVKRTDGFITKFNRLIERRL
                     LRHFSKKGYITWEEN"
     gene            3012..4112
                     /gene="yqeH"
                     /locus_tag="POB55_001549"
     CDS             3012..4112
                     /gene="yqeH"
                     /locus_tag="POB55_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HDI6500513.1"
                     /translation="MSDILKCIGCGAPLQSEDKNKPGFVPEHNMFRDDVICRRCFRLK
                     NYNEVQDVGLESEDFLKLLSGLADKKGIVVNVVDVFDFEGSFINAVKRIVGNKKIILA
                     ANKLDLLPKQINKRRVKEWLKRTARKYGLEADDVVLISAEKGWGIDDLLSSIANIREN
                     EDVYIVGTTNVGKSTLINKLIEASVGEKDVVTTSRFPGTTLDMIDIPLDETSFMYDTP
                     GIIQDHQMTHLVSEKELKIIMPKKEIKQRVYQLNEAQTLFFGGLARIDYVSGGKRPLV
                     CFFSNDLNIHRTKTEKANDLWRNQLGDLLTPPGNPQNFDLNEVKAVRLETGKEKRDVM
                     ISGLGFITIGPGAKVIVRVPKNVDVVLRNSIL"
     gene            4126..4932
                     /gene="aroE"
                     /locus_tag="POB55_001550"
     CDS             4126..4932
                     /gene="aroE"
                     /locus_tag="POB55_001550"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDI6500514.1"
                     /translation="MKFAVIGNPISHSLSPVMHRANFNSLGLDDTYEALNIPIEDFHL
                     IKEIISKKELDGFNITIPHKERIIPYLDHVDEQAINAGAVNTVLIKDDKWIGYNTDGI
                     GYVKGLHSVYPDLENAYILILGAGGASKGIAYELAKFVKPKLTVANRTMARFESWNLN
                     INQISLADAEKYLAEFDIVINTTPAGMAGNNESIINLKHLSPNTLMSDIVYIPYKTPI
                     LEEAERKGNHIYNGLDMFVYQGAESFKIWTNKDADINSMKTAVLQQLKGE"
     gene            4936..5226
                     /gene="yhbY"
                     /locus_tag="POB55_001551"
     CDS             4936..5226
                     /gene="yhbY"
                     /locus_tag="POB55_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HDI6500515.1"
                     /translation="MLTGKQKRYLRSLAHNIDPIFQIGKGGINENMIKQIDDTLENRE
                     LIKVHVLQNNFDDKKELAETLSEATRSELVQVIGSMIVIYRESKENKEIELP"
     gene            5229..5798
                     /gene="nadD"
                     /locus_tag="POB55_001552"
     CDS             5229..5798
                     /gene="nadD"
                     /locus_tag="POB55_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HDI6500516.1"
                     /translation="MKKIVLYGGQFNPIHTAHMIVASEVFHELQPDEFYFLPSFMSPL
                     KKHNNFIDVQHRLTMIQMIIDELGFGDICDDEIKRGGQSYTYDTIKAFKEQHKDSELY
                     FVIGTDQYNQLEKWYQIEYLKEMVTFVVVNRDKNSQNVENAMIAIQIPRVDISSTMIR
                     QRVSEGKSIQVLVPKSVENYIKGEGLYEH"
     gene            5788..6372
                     /gene="yqeK"
                     /locus_tag="POB55_001553"
     CDS             5788..6372
                     /gene="yqeK"
                     /locus_tag="POB55_001553"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HDI6500517.1"
                     /translation="MNIEKAKRLAKEKLPEKRYNHSLRVAETAIKLAEIYDGDTSKVE
                     LAGVLHDFCKYDDLGKMYQIVRQYELGNDLLSYGSEILHGPVCAAIMEHEYGINDEEV
                     LMAIKYHTTGRQQMTKTEKLIFIADYIEPGRTIPGVDDIRDMAYNQGSLDKTIYEISK
                     RTVLFLIQKDITVYNKTIDCLNYYNYSDERIKDD"
     gene            6373..6726
                     /gene="rsfS"
                     /locus_tag="POB55_001554"
     CDS             6373..6726
                     /gene="rsfS"
                     /locus_tag="POB55_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HDI6500518.1"
                     /translation="MNSQELLAIAVDAIDNKKGEDTISLEMKGISDMTDYFVVTHGNN
                     ERQVQAIARAVKEVANEQNIEVKRMEGYNEARWILIDLADVVVHVFHKDERNYYNIEK
                     LYQDAPLESYSQVAY"
     gene            6729..7445
                     /locus_tag="POB55_001555"
     CDS             6729..7445
                     /locus_tag="POB55_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDI6500519.1"
                     /translation="MSQYAEMSLVYDQLTQDQPYEKWFEIVKNHCKDESNILDIGCGT
                     GSLTVQLEALGNVTGMDLSVDMLTVAAQKSANVNWLEGDMTSFDLQQQFDIITIFCDS
                     LNYLQDETAVIETFINVYHHLTDSGVFIFDVHTVYKMMTLFNNQSYIDDKGDIFLAWD
                     AVQGDLPLSVYHDMTFFIRHEDETYSRFDESHFQRTFDEKTYLSWLAQVGFKHVETFT
                     DFNIDEHNEDAERLFFIAKK"
     gene            7485..8171
                     /locus_tag="POB55_001556"
     CDS             7485..8171
                     /locus_tag="POB55_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001802022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HDI6500520.1"
                     /translation="MVLLYQFLLRYKDFLTQWKLYIISAVVLIMVLIGFIFWRQDDYT
                     SRNFENKDNALKQSTSENSSLSKVEDVQVKDGDNSKNKGPVYVDVKGAVKHPNVYKMT
                     SKDRVVDLLDKAQLLEDADVSQINLSEKLTDQKMIFIPHKGQKNVEPQIGVNSVHVKN
                     GNTNNTKVNLNTASVSELMSVPGVGQAKANAIVEYRNQQGAFQEIDDLKKVKGFGSKT
                     FDKLKSYFTI"
     gene            8263..8724
                     /locus_tag="POB55_001557"
     CDS             8263..8724
                     /locus_tag="POB55_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000439692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HDI6500521.1"
                     /translation="MERIKWEEYFMAQSHLLALRSTCQRLSVGATIVKDNRIIAGGYN
                     GSVAGEVHCIDEGCLIEDGHCIRTIHAEMNALLQCAKQGVSTEGATIYVTHFPCLNCT
                     KSIIQAGIKRIYYAEDYHNHEYATKLLKQSGIEFKKIPFSPEYVAKYLTKG"
     gene            8729..10930
                     /locus_tag="POB55_001558"
     CDS             8729..10930
                     /locus_tag="POB55_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HDI6500522.1"
                     /translation="MLYVALSMIVGVLWNSSKVLSTFLFILLLYITYRKNKIVYAPIS
                     LFLIIFSSWYLHYSQQAIFNYINYIERNSQFNERAQVIQIQRQGSDTYKGRLSLKNEI
                     YPFFLTNKKNFDLKKIESRNCIVKGQFKVNDNKFVTLKLQSIVVQSCLESNRSNLIEK
                     HKQFIMNRIYDSGIKFPDRIMALITGDVKEINEQFKERVKEIGIYHLLAVSGSHIAAI
                     VFLIYKPLKRLNLPLFVIKGITIIVLALFAQYTNYAPSAVRAIIMTTLVLLITKQIKI
                     KGIQLLAFAFIIMFILNPLVVYDIGFQFSFIISFFIMLLFPFLQQLSKLQSLFIITFI
                     AQLASFIVAIPNFHQLQWVGFLSNLIFVPYYSIILFPLSILFFITSHFIVGLTPLNYL
                     VDLSFNFHDWLLDLFTRIKQSHFSVTKFNDWIFIIFIISVYYIFWLLAKRKYILVTVW
                     TIIILTLLITFPTNSHHKITMLNVGQGDSILYEGGKNQNVLIDTGGKVIDDTKQPSYS
                     ISKYHILPTLNERGINELEYLILTHPHNDHIGEVEYIISHIKIKHIEIYNKGYSSNTL
                     MLLSKLSHKYNIKLIDVRQVSSFKLGDSSFLFFDSFIPNSRDKNEYSIITMITYQNKK
                     VLLMGDASKNNESLLLKKYNLPEIDILKVGHHGSKTSSSKEFIEMIKPKISLISSGKN
                     NMYHLPNIEVVKRLQRIRSRIYNSQQNGQVTIDLDDNLKVDSNSYGNASGL"
     gene            10987..>11904
                     /gene="holA"
                     /locus_tag="POB55_001559"
     CDS             10987..>11904
                     /gene="holA"
                     /locus_tag="POB55_001559"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HDI6500523.1"
                     /translation="MSDNIVAIYGDVPELVEKQSAEIISQFLKSDRDDFNFVKYNLYE
                     TEIAPIVEETLTLPFFSDKKAILVKNAYIFTGEKAPKDMAHNVNQLIEFIEKYDGENL
                     IVFEIYQNKLDERKKLTKTLKKHARLKKIEQMSEEEIKKWIQSKLNENFKDIKRDALD
                     LFIELTGINFNIVSQEIEKLILFLGDRPTINKQDVNQIINRSLEQNVFLLTEYIQKRK
                     KEQAIHLVKDLITMKEEPIKLLALITSNYRLFYQCKILSQKGYSGQQIAKTIGVHPYR
                     VKLALGQVRHYQLDELLNIIDACAETDYKL"
BASE COUNT         4420 a         1475 c         2135 g         3874 t
ORIGIN      
        1 atttctatag aaaaagtatt tctttatcgt cgtcccaccc caacttgcac attatcgtaa
       61 gctgactttc cgccagcttc tatgttgggg cccctgactt gaattgaaaa aaacttgtta
      121 caagcgcatt ttcgttcagt caactactgc caatataact tcgtagagca tagaacattg
      181 atttatttcc cagcctgttt atcatttatg tctcgctctc aaatgtaaat taataattgt
      241 tttggggagt ttgaagttaa atatttaaca ggatttattt taatattatt gttagaagaa
      301 attttgacaa attcagcaag tgcaatcgaa tattcagact tacatcataa aagtaagttt
      361 gattcaaagc gtctaagtaa tgctaagatg tcattcatca atccaactca gcttgaaaat
      421 aaaaacacaa acgatagact gttgaagcat gatttgttat ttcatgacat gttcgtaaat
      481 gatgattgga aaaaagattt taaagttgaa ttcgaaaatg aggcactttc aaagaaattt
      541 ataaataagg atatcgatat atttgctgga aattatggat atggatgtca tgggggagca
      601 accaataaaa cgcaatgtag ttatggtggt gttactttaa gtgacaataa taagtacgat
      661 gattataaga atataccttg taatttatgg attgacggac atcaagcaga aatagaatta
      721 acagcagtaa aaacgaaaaa gaaaattgtt accattcaag aattagatgt tcaattaaga
      781 aattatttga atgagaagta taagttgtac gaacaaggtg gcgacattgt taaagggtat
      841 gttaaatatc ataatgatga tgaacaaaat atagaatata atttttataa tttaaatggt
      901 gagtatggtt atgaggtatt aaaaatgtat gctgataata aaactatcaa tagtgacaaa
      961 ttgcatttag atatctattt attcaaatca taatttaatt aagttaatca ttaataatct
     1021 ttgacagtga aatataaagt acctgtcatt atactgttac aaataaaaac agcagtaagg
     1081 atgataacac ttagaaaagt cttactgctg ttttctatta gaattatctt taatacacac
     1141 aggaattcga tacgaaacaa atatgattaa ctgtgtaatt acaaaataaa actactatag
     1201 taaaaagaag ataaagacga tgataataaa tgcatcgaat ataattttct tgatgttttt
     1261 atcaacaaca tcaggtaagg acataagtat aaatcctaga attagagtgt agcgacatat
     1321 gttcataatt gttgctgtaa actcgatatt aaatgaaaac tttaaaaata tacttataaa
     1381 taaaatgaca aacgacgcaa ttgcaataaa actgccgtat gtagctatct ttgttacgag
     1441 actattttga ttattttcag ccattgtaaa atcctcttat ttcatcatta ttattttaaa
     1501 catatcattt tcctgttatt taataaagtt tcttgacacg attggttgat gtaattgata
     1561 tgattagttt ttgaaaaata gacaaattag aagcatcgaa ttcactgtta tgagttcaaa
     1621 aataattatt tatgatacca agttattgaa agagcttttt ataaaaataa agcaaaagtt
     1681 ttatacagta atgaaaatca tgatacttgt cgttgtagtg attaaaatgt gataaaataa
     1741 caaatgctag gattttttaa aagaggtgaa tcaaaggatg attggtataa ttggtgccat
     1801 ggaagaagaa gtaacaatat taaaaaataa attaacacaa ttaagcgaaa tttcagttgc
     1861 acatgttaaa ttttatactg gcattttaaa agatagagaa gtagtgatta cccaaagcgg
     1921 cattggaaaa gttaatgctg caatttctac gacattatta attaataagt ttaaaccgga
     1981 cgtcattatt aatacaggtt ctgctggagc tttagatgaa agtttaaatg taggtgacgt
     2041 tcttataagt gatgatgtaa aatatcatga tgcggatgca acagcatttg gttatgaata
     2101 tggacaaata ccacagatgc cggtagcatt tcaatcaagt aaacctttaa tagaaaaagt
     2161 atctcaagtt gtacaacaac aacaattaac agctaaagta ggcttaattg taagtggtga
     2221 tagctttatc ggtagtgttg aacaacgtca aaaaattaaa aaagcatttc caaatgcgat
     2281 ggcggttgaa atggaagcaa ctgcaattgc acaaacatgt tatcaattta atgtaccatt
     2341 tgttgtagtt cgtgcagttt cagacttagc aaatggagaa gcggaaatga gcttcgaagc
     2401 atttttagaa aaagcagctg tatcatcaag tcaaactgtt gaagcattag tgtctcaatt
     2461 ataaaaaggt aaaggtgagt taaatgggtt tagttcgcaa gttttttatg ccgaattcat
     2521 atgttcaatc aatatttcaa attgatttag acaagttagt ggacaaaggc gttaaaggta
     2581 ttattacaga tttagataat acgctagtag gttgggatgt taaagaacct acagaacgtg
     2641 ttaaagcatg gtttaaggaa gctaatgaaa aaggaatcac tattacaatc gtgtctaata
     2701 ataatgagtc tcgtgttgct agttttagtc agcatttaga catcgatttt atttttaaag
     2761 cgagaaagcc aatggggaaa gcgtttgata aagcaataac taagatgaat atcagaccag
     2821 atcaaactgt tgttataggt gaccaaatgc ttactgatgt atttggtggt aatcgtcgag
     2881 gtctatatac aattatggtt gttccagtta aacgaactga tggctttatt actaagttta
     2941 atagattaat tgaaagacga ttattacgtc atttcagtaa aaaaggttat atcacatggg
     3001 aggaaaattg attgtctgac attttaaaat gtatcggttg tggtgcgcca cttcaatctg
     3061 aagataaaaa taaacctggt tttgtaccag agcataatat gtttcgtgat gacgtgattt
     3121 gcagacgttg tttccgcttg aaaaattata acgaagttca agatgtagga ttagaaagtg
     3181 aagacttttt aaaattatta tcaggacttg cggataaaaa gggtattgtc gtcaatgtcg
     3241 tggatgtatt tgactttgaa ggatcattta ttaatgcagt taaacgtatt gtcggaaaca
     3301 aaaaaatcat tttagcagca aataaattgg atttattgcc gaaacaaatt aataaacgtc
     3361 gagttaaaga atggttaaaa cgaacagcaa gaaaatatgg tttggaagct gacgatgtcg
     3421 tattaatttc agctgaaaaa ggctggggca tagacgactt attatcatca attgcgaata
     3481 ttcgagaaaa tgaagatgtg tatattgtag ggacaacgaa tgttgggaaa tctacattga
     3541 ttaataaact gattgaagct agtgttggtg aaaaagatgt agtaacaact tcaagattcc
     3601 ctggaacaac tttagatatg atagatattc ctttagatga aacatcattt atgtatgata
     3661 caccaggtat tattcaagat caccaaatga cgcatttagt tagtgaaaaa gaattgaaaa
     3721 ttattatgcc taagaaagaa ataaaacaac gcgtatatca attaaatgag gcgcaaacat
     3781 tattcttcgg cggtctagcg cgcatagatt atgtatcagg tggtaaacgt ccgttagttt
     3841 gtttcttttc taatgacttg aatatacatc gtactaaaac ggagaaggcc aatgatttat
     3901 ggcgtaatca acttggcgat ttattaacgc cacctggaaa tccacaaaat tttgatctta
     3961 atgaggtaaa ggctgttaga cttgaaacag gcaaagagaa acgcgatgtt atgatctctg
     4021 gtctaggctt tataactata ggaccaggag ctaaagtaat cgttcgtgtt cctaaaaatg
     4081 ttgatgttgt attaagaaat tctattttat aaggtgattt aaaaaatgaa atttgcagtt
     4141 atcggaaatc ctatttcaca ttccttgtcg cccgttatgc atagagcaaa ttttaattct
     4201 ttaggattag atgatactta tgaagcttta aatattccaa ttgaagattt tcatttaatt
     4261 aaagaaatta tttcgaaaaa agaattagat ggctttaata tcacaattcc tcataaagaa
     4321 cgtatcatac cgtatttaga tcatgttgat gaacaagcga ttaatgcagg tgcagttaac
     4381 actgttttga taaaagatga caagtggata gggtataata cagatggtat tggttatgtt
     4441 aaaggattgc acagcgttta tccagattta gaaaatgcat acattttaat tttgggcgca
     4501 ggtggtgcaa gtaaaggtat tgcttatgaa ttagcaaaat ttgtaaagcc caaattaact
     4561 gttgcgaata gaacgatggc tcgttttgaa tcttggaatt taaatataaa ccaaatttca
     4621 ttagcagatg ctgaaaagta tttagctgaa ttcgatatcg ttattaatac aacaccagcg
     4681 ggtatggctg gaaataacga aagtattatt aatttaaagc atctttctcc caatacttta
     4741 atgagtgata ttgtttatat accgtataaa acacctattt tagaggaagc agagcgcaag
     4801 ggaaaccata tttataatgg cttagatatg tttgtttacc aaggtgcgga aagctttaaa
     4861 atttggacta ataaagatgc tgatattaat tctatgaaaa cagcagtttt acaacaatta
     4921 aaaggagaat aacatatgct tactggcaaa caaaaaagat acttaagaag tttagcacac
     4981 aatattgatc cgatttttca aattggaaaa ggcggtatca acgaaaatat gattaaacaa
     5041 atagatgata cgttagaaaa cagagaattg attaaagtac atgtactaca aaataacttt
     5101 gatgataaaa aagaattagc tgaaacatta agcgaagcta cgcgtagtga attagtgcaa
     5161 gtgattggat ctatgatagt gatttataga gaatctaaag aaaataaaga aattgaattg
     5221 ccataataat gaaaaagata gtactttacg gcggtcagtt taaccctatc catactgcac
     5281 atatgatagt agctagcgaa gtatttcatg aattacagcc agatgaattt tattttttac
     5341 ctagttttat gtctccattg aaaaagcaca ataattttat agatgttcag cacagattaa
     5401 caatgataca gatgattatc gacgagcttg gttttggaga tatttgcgac gatgaaatta
     5461 aacgtggtgg tcagagttat acctatgaca cgatcaaggc attcaaggag caacacaaag
     5521 acagtgagtt gtactttgtt attgggacgg atcagtataa ccaactagag aaatggtatc
     5581 aaattgaata cttaaaagaa atggttactt ttgtagttgt aaatcgagac aaaaatagtc
     5641 aaaatgttga aaatgctatg attgcaattc agatacctag ggtagatata agttcgacaa
     5701 tgattcgaca aagagttagt gaagggaaat ctatccaagt tcttgttcct aaatccgttg
     5761 aaaactatat taagggggaa ggattatatg aacattgaaa aagcaaaacg gcttgcaaaa
     5821 gagaaactgc cagagaaacg ttataatcat tctttaagag ttgcagaaac agctattaaa
     5881 ttggcagaaa tttatgatgg agacactagt aaagtagaat tagcaggtgt attacatgat
     5941 ttctgtaaat atgatgattt aggtaaaatg tatcaaattg ttcgacaata cgaattaggt
     6001 aatgatctac tgagttatgg tagtgaaata ttgcatggcc ctgtgtgtgc agcaatcatg
     6061 gaacatgaat atggtatcaa tgatgaggaa gtattaatgg ctatcaaata ccatactact
     6121 ggacgtcaac aaatgacaaa aactgaaaaa ctgattttta ttgcagatta catcgaacct
     6181 ggaagaacaa tcccaggagt tgatgatatt cgagatatgg catacaatca aggtagttta
     6241 gataagacaa tttatgaaat ttctaaacgc acagtactat ttttaataca gaaagatatt
     6301 acggtatata ataagacgat tgactgttta aattattata actatagtga cgaaagaata
     6361 aaggatgatt aaatgaattc acaagaatta ttagcaattg ctgtggatgc aattgacaat
     6421 aaaaaaggcg aagatacgat ttctttagaa atgaaaggta tcagcgatat gacagattat
     6481 tttgttgtaa cgcacggaaa taatgaacga caagttcaag cgattgctag agcggtgaaa
     6541 gaagtagcca atgaacaaaa tatagaagta aaacgtatgg aaggatacaa tgaagcgcgt
     6601 tggatattaa ttgacttagc tgatgttgtg gtacatgttt tccataaaga cgaaagaaat
     6661 tattataata ttgaaaagtt atatcaagat gcaccattag aatcatatag tcaggttgcg
     6721 tattaattat gtcgcaatat gcagaaatga gcctagtgta cgatcaattg actcaagatc
     6781 aaccatatga aaaatggttt gaaattgtaa aaaatcactg caaagatgaa tcaaatattt
     6841 tagatattgg atgcggtact ggtagtttaa cagttcaatt agaagcttta ggtaatgtta
     6901 ctggtatgga tttaagtgtt gatatgttga ctgttgcagc tcaaaaatca gcaaatgtaa
     6961 attggcttga aggagatatg acatcttttg atttgcaaca acaatttgat atcatcacta
     7021 ttttttgtga ctctctaaat tatttgcaag atgaaactgc agtgattgaa acattcatca
     7081 atgtttatca tcatctgact gattcaggtg tatttatttt tgatgtacat actgtttata
     7141 aaatgatgac tttgtttaat aatcaaagtt atattgatga taaaggggac atttttttag
     7201 catgggatgc tgtgcaagga gatttacctt taagtgttta tcatgatatg acatttttca
     7261 ttcgacatga agatgaaaca tattcaagat ttgatgagtc tcattttcag agaacatttg
     7321 atgaaaagac atatttgtct tggttagctc aagttggttt taaacatgtt gaaacattta
     7381 ctgattttaa tatagatgaa cataatgaag atgcagaaag attgtttttc attgcgaaaa
     7441 aataaaagta aaaccactcg ctcagacgtg tataaagttg aggagtggtt ttattgtatc
     7501 aatttttatt acgctataaa gattttttaa ctcaatggaa gttatatatt ataagtgctg
     7561 ttgttttaat tatggtatta attggtttta tattctggag acaagatgat tatacttcaa
     7621 gaaattttga aaataaagat aatgctctga aacaaagcac tagtgaaaat agtagtttgt
     7681 ccaaagtaga agatgtccag gtcaaagatg gagataattc caaaaataag ggtcctgtat
     7741 atgtcgatgt aaaaggtgct gttaaacatc ctaatgttta taaaatgaca tctaaggata
     7801 gagtagttga tttacttgat aaagcacaat tattggagga tgcagatgta agtcaaatta
     7861 atttgtctga aaaattaaca gatcaaaaaa tgattttcat acctcataaa ggacaaaaga
     7921 atgttgaacc acaaattgga gtaaacagtg tgcatgtaaa aaatgggaac acaaataata
     7981 ctaaagtaaa tttaaatacc gcatctgtat cagaattgat gtctgttcct ggagtagggc
     8041 aagctaaagc taatgcaatt gttgaatatc gcaaccaaca aggtgcattt caagaaattg
     8101 acgatttgaa aaaagtaaaa ggttttggaa gtaaaacttt tgataaactg aaatcttatt
     8161 tcacgatata atggattatt ttaattgcaa attttatttt ttgtgattaa tcttatatta
     8221 atagaaaatg ttactaaatt ttaagtatgg aaggtgaaca tcttggaaag aatcaaatgg
     8281 gaagaatatt ttatggcaca aagtcatttg ctagcattac gttcaacttg tcaaagatta
     8341 tctgtaggtg caacgattgt taaggataat cgtattattg ctggtggtta taatggctct
     8401 gtagctggcg aggtgcattg tatagatgaa ggatgtttaa ttgaagatgg acattgtatc
     8461 agaacgatac atgcagaaat gaatgcttta ttacaatgtg caaaacaagg tgtatctact
     8521 gaaggtgcaa caatctatgt tactcatttt ccatgcctaa attgtacaaa gtcaattatt
     8581 caagcaggta taaagcgtat ctactatgca gaagattatc ataaccatga atatgcaact
     8641 aaattactca aacaatctgg tattgaattt aaaaaaattc cattttcacc agaatatgtt
     8701 gctaaatatc tgactaaagg ttaatgtttt gctgtatgtc gcgttatcaa tgattgtagg
     8761 agtgctttgg aattctagca aagtgctctc tacatttctt ttcattttac ttttgtatat
     8821 tacttatcgt aaaaataaaa tcgtttatgc ccctatttct ctctttttaa tcattttctc
     8881 ctcatggtat ttacattatt cacaacaagc aatatttaat tatatcaatt atattgaacg
     8941 taattctcag tttaatgagc gtgctcaagt aatccaaatt caacgtcaag gtagtgacac
     9001 atataaaggt aggttgagtt taaaaaatga aatatatcct ttctttttaa caaataaaaa
     9061 gaattttgat ttaaagaaaa ttgaaagtcg taattgtatt gttaaaggac aattcaaagt
     9121 taatgacaat aagtttgtaa ctcttaaatt acaaagtata gttgtacaaa gctgcctaga
     9181 atcgaaccgg tctaatttaa ttgagaaaca taaacagttt ataatgaatc gaatttatga
     9241 ttcgggtatt aagtttccgg atcgtattat ggcattgatt actggtgacg taaaagaaat
     9301 taatgagcaa tttaaggaac gtgttaaaga gataggtata tatcatttgc tggcagttag
     9361 tggctcgcat atagctgcaa ttgtattctt aatttacaaa cctttaaaac gattaaattt
     9421 acctttattt gtcattaaag gaattacaat cattgtatta gctttatttg ctcaatacac
     9481 aaattatgca cctagtgctg taagagctat aataatgaca actcttgtac tgcttattac
     9541 taagcaaatt aaaataaagg gtattcagct attagcattt gcatttataa ttatgtttat
     9601 tttaaatcca ctagttgttt atgatattgg atttcaattt tcattcatca tttcattttt
     9661 tattatgcta ctttttcctt ttttacagca attgtcaaag ttacaatcat tattcataat
     9721 tacgtttatt gcacaattag cttcatttat cgttgccatt ccaaactttc atcaacttca
     9781 atgggtggga tttttatcta atttgatttt tgtaccgtac tattcgatta tattgtttcc
     9841 gctatctatt ttattcttta ttacaagtca ttttattgtg ggattaacgc cgctaaatta
     9901 cttggttgac ctaagtttta attttcatga ctggttacta gacctattca caagaatcaa
     9961 gcaatcacat ttttctgtta ccaagtttaa tgattggata tttataatat ttataatttc
    10021 tgtttattac atattttggt tattggctaa acgtaaatat atattggtta cggtttggac
    10081 tataattatt ctgacattat taataacgtt tccaacaaat tcacatcaca aaattacaat
    10141 gttaaatgtg gggcagggag acagtatttt atatgaaggt ggtaagaacc aaaatgtctt
    10201 gattgataca ggtgggaaag tgattgatga tactaaacaa cctagttatt caatttctaa
    10261 atatcatatt ttaccaacgc taaatgaaag agggataaat gaattagagt atctaatttt
    10321 aacacatcca cacaatgacc atattggtga agtggaatat attattagtc atattaaaat
    10381 taaacatata gagatataca ataagggata tagtagtaat acattgatgt tattatcgaa
    10441 attaagccat aagtacaaca ttaaacttat agatgtaaga caagttagta gttttaaact
    10501 tggagatagt agttttctat tttttgatag ttttattcca aatagccgag ataaaaatga
    10561 gtattcgatt attactatga ttacatatca aaataaaaaa gttttattaa tgggcgatgc
    10621 tagtaaaaat aatgaatctt tactactaaa aaaatataac ttgccggaga ttgatatttt
    10681 aaaagtaggt catcatggga gcaagacaag tagttctaaa gaatttatag agatgattaa
    10741 gcctaaaata agtttgattt cttctgggaa gaacaatatg tatcatcttc ctaatataga
    10801 agttgttaaa cgattgcaaa ggattcgcag tcgcatttac aatagtcaac aaaacggtca
    10861 agttacaatt gacttagatg ataatttaaa agttgattca aactcttatg gaaatgcaag
    10921 tggtttatag atacagaaac atgatataat ctattgatgt gtcaaacgaa tgaaagggtg
    10981 taatgaatga gcgacaatat tgtagctatt tatggagatg tgcctgaatt ggttgaaaaa
    11041 caaagtgcag aaattatatc acaatttttg aaaagtgata gagatgactt taactttgtg
    11101 aaatataatt tatacgaaac agagattgca ccaattgttg aagaaacatt aacattgcct
    11161 ttcttttcag ataaaaaagc aattttggtt aaaaatgcat atatatttac aggtgaaaaa
    11221 gcgccaaaag atatggctca taatgtaaat caattaatag aatttattga aaaatatgat
    11281 ggcgaaaatt tgattgtctt tgagatatat caaaataaac ttgatgaaag aaaaaagtta
    11341 actaaaactc taaaaaagca tgcaaggctt aaaaaaatag agcaaatgtc tgaagaagaa
    11401 ataaaaaaat ggattcaaag taaattaaat gagaatttca aagatatcaa aagagatgca
    11461 ttagatttat ttattgagtt gacaggtatt aactttaata ttgtctcaca agagatagaa
    11521 aagttgattt tatttttagg cgatagacca acaattaata agcaggatgt taaccaaatt
    11581 attaatagaa gtttagagca aaatgtattt ttactgactg aatacattca gaaaagaaag
    11641 aaagaacaag caattcattt agtaaaagat ttaataacta tgaaagaaga acctattaaa
    11701 ttacttgcac taatcacaag taattaccga ttattttatc aatgtaagat tttgagtcaa
    11761 aaaggatata gtggacagca aattgctaaa acaataggcg tacatccata cagagtaaaa
    11821 ttagcgttag gacaagtaag acattatcaa cttgatgaat tattgaatat tatagatgct
    11881 tgtgcggaaa ctgattataa actt
//
LOCUS       DAMZKC010000079        11849 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.079, whole genome shotgun sequence.
ACCESSION   DAMZKC010000079 DAMZKC010000000
VERSION     DAMZKC010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11849
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.079"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            109..951
                     /locus_tag="POB55_001560"
     CDS             109..951
                     /locus_tag="POB55_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDI6500524.1"
                     /translation="MKIGDISIHYLNGGNTKMDGGAMFGVVPKPLWSKQYNANERNQI
                     NLPTHPILIQTAQYNLIIDAGIGNGKLSEKQLRNFGVDEESHIITDLANYNLTPKDID
                     YVLMTHMHFDHAAGLTDQAGHAIFENAIHVVQQDEWHEFIATNIRSKSTYWDKNKGDY
                     SNKLILFEKHFEPVPGIKMQHSGGHSFGHTIITIESQGDKAVHMGDIFPTTAHKNPLW
                     VTAYDDYPMQSIREKERMIPYFIQQQYWFLFYHDENYFAVKYSDDGENIDAYILRETL
                     VDNN"
     gene            complement(1076..1387)
                     /locus_tag="POB55_001561"
     CDS             complement(1076..1387)
                     /locus_tag="POB55_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDI6500525.1"
                     /translation="MTKLKYIIPTIIAVAIVIISTISIIQYINRKRYNPVKVLNEVKS
                     YFMNVKGSYIVYEPFVHPETDKYRLVYQGGITTIKNGQNIHYDFYADAYTGEVINIVE
                     R"
     gene            1474..2550
                     /locus_tag="POB55_001562"
     CDS             1474..2550
                     /locus_tag="POB55_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HDI6500526.1"
                     /translation="MNINKKVTLQRIQTLTELHGAPGFEEEVKNYMTQQMAPYVDEFI
                     ENRMGGFFGVKKSKNPNAKRVMIAAHMDEIGFMITNITKNGMIQFTNLGGVANDIWQG
                     QRLVIKNRNGDKIIGVVSNIPKHFRTGSEGAPEIKDLTLDIGAQNEDEVRERGIDIGD
                     TIVPHTPFTQLSEHRYSAKAWDNRYGCVLAIEILELLKDIELDVDLYVGANVQEEVGL
                     RGAKASAEMIDPDVAFVVDCSPANDVKGSQPLSGELGKGTLIRIKDGTMILKPVFRDY
                     LLKLVEAHDIEHQYYMSPGGTDGGEIHKANIGIPTAVIGVCARYIHSTDSVFDIRDYF
                     AARSLLSEAICNLDNNQIETLQYK"
     gene            2615..2926
                     /locus_tag="POB55_001563"
     CDS             2615..2926
                     /locus_tag="POB55_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDI6500527.1"
                     /translation="MKQLESEQQFESLKQGATVFEFTAGWCPDCRVIEPDLPELEARY
                     PMFDFVSVDRDKFMDICIENGIMGIPSFLVYKNGELLGSYIGKERKSIEQIDAFLAQY
                     V"
     gene            3026..3883
                     /locus_tag="POB55_001564"
     CDS             3026..3883
                     /locus_tag="POB55_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1444 domain-containing protein"
                     /protein_id="HDI6500528.1"
                     /translation="MNTFQMRDKLKERLSHLDVDFKFNREEETLRIYRTDNNKGITIK
                     LNAIVAKYEDKKEKIVDEIVYYVDEAIAQMADKTLESISSSQIMPVIRATSFDKKTKQ
                     GVPFIYDEHTAETAVYYAVDLGKSYRLIDESMLEDLKLTEQQIREMSLFNVRKLSNSY
                     TTDEVKGNIFYFINSNDGYDASRILNTAFLNEIEAQCQGEMLVAVPHQDVLIIADIRN
                     KTGYDVMAHLTMEFFTKGLVPITSLSFGYKQGHLEPIFILGKNNKQKRDPNVIQRLEA
                     NRRKFNKDK"
     gene            3912..4508
                     /locus_tag="POB55_001565"
     CDS             3912..4508
                     /locus_tag="POB55_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HDI6500529.1"
                     /translation="MNLFYNPKYVGDVAFLQIEPVEGELNYNKKGNVVEITNEGNVVG
                     YNIFEISKDITIEEKGHIKLTDELVNVFQKRISEAGFDYKLNADLSPKFVVGYVETKD
                     KHPDADKLSVLNVNVGNDTLQIVCGAPNVEAGQKVVVAKVGAVMPSGMVIKDAELRGV
                     ASSGMICSMKELNLPNAPEEKGIMVLNDSYEIGQAFFE"
     gene            4529..8353
                     /locus_tag="POB55_001566"
     CDS             4529..8353
                     /locus_tag="POB55_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDI6500530.1"
                     /translation="MSWFDKLFGEDNDSNDDLIHRKKKRRQESQNIDNDHDSLLPQNN
                     DIYSRPRGKFRFPMSVAYENENVEQSADTISDEKEQYHRDYRKQSHDSRSQKRHRRRR
                     NQTTEEQNYSEQRGNSKISQQSIKYKDHSHYHTNKPGTYVSAINGIEKETHKSKTHNM
                     YSNNTNHRAKDSTPDYHKESFKTSEVPSAIFGTMKPKKLENGRIPVSKPSEKVESDKQ
                     KYDKYVAKTQTSQNKQLEQEKQNDSVVKQGTASKSSDENVSSTTKSMPNYSKVDNTIK
                     IENIYASQIVEEIRRERERKVLQKRRFKKALQQKREEHKNEEQDAIQRAIDEMYAKQA
                     ERYVGDSSLNDDSDLTDNSTDASQLHTNGIENETVSNDENKQASIQNEDTNDTHVDES
                     PYNYEEVSLNQVSTTKQLSDDEVTVSNVTSQHQSALQHNVEVNDKDELKNQSRLIADS
                     EEDGATNKEEYSGSQIDDAEFYELNDTEADEDTTSNIEDNTNRNASEMHVDAPKTQEH
                     AVTESQVNNIDKTVDNEIELAPRHKKDDQTNLNVNSLKTNDVNDGHVVEDSSMNEIEK
                     HNAEITENVQNEAAESEQNVEEKTIENVNPKKQTEKVSTLSKRPFNVVMTPSDKKRMM
                     DRKKHSKVNVPELKPVQSKQAVSESKPASQAAPSSRFDSQESNTNAYKTNNMTSNNVE
                     NNQLIGHAETENDYQNAQQYSEQKPSADSTQTEIFEESQDDNQLEDEQVDQSTSSSVS
                     EVSDITEESEETTHPNNTSGQQDNDDQQKDLQPSFSNQNEDTANENRPRTNQQDVATN
                     QAVQTSKPMIRKGPNIKLPSVSLLEEPQVIESDEDWITDKKKELNDALFYFNVPAEVQ
                     DVTEGPSVTRFELSVEKGVKVSRITALQDDIKMALAAKDIRIEAPIPGTSRVGIEVPN
                     QNPTTVNLRSIIESPSFKNAESKLTVAMGYRINNEPLLMDIAKTPHALIAGATGSGKS
                     VCINSILMSLLYKNHPEELRLLLIDPKMVELAPYNGLPHLVAPVITDVKAATQSLKWA
                     VEEMERRYKLFAHYHVRNITAFNKKAPYDERMPKIVIVIDELADLMMMAPQEVEQSIA
                     RIAQKARACGIHMLVATQRPSVNVITGLIKANIPTRIAFMVSSSVDSRTILDSGGAER
                     LLGYGDMLYLGSGMNKPIRVQGTFVSDDEIDDVVDFIKQQREPDYLFEEKELLKKTQT
                     QSQDELFDDVCAFMVNEGHISTSLIQRHFQIGYNRAARIIDQLEQLGYVSSANGSKPR
                     DVYVTEADLNKE"
     gene            8377..9690
                     /locus_tag="POB55_001567"
     CDS             8377..9690
                     /locus_tag="POB55_001567"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HDI6500531.1"
                     /translation="MTHYHFVGIKGSGMSSLAQIMHDLGHEVQGSDIENYVFTEVALR
                     NKGIKILPFDANNIKEDMVVIQGNAFASSHEEIVRAHQLKLDVVSYNDFLGQIIDQYT
                     SVAVTGAHGKTSTTGLLSHVMNGDKKTSFLIGDGTGMGLPESDYFAFEACEYRRHFLS
                     YKPDYAIMTNIDFDHPDYFKDINDVFDAFQEMAHNVKKGIIAWGDDEHLRKIEADVPI
                     YYYGFKDSDDIYAQNIQITDKGTAFDVYVDGEFYDHFLSPQYGDHTVLNALAVIAISY
                     LEKLDVTNIKEALETFGGVKRRFNETTIANQVIVDDYAHHPREISATIETARKKYPHK
                     EVVAVFQPHTFSRTQAFLNEFAESLSKADRVFLCEIFGSIRENTGALTIQDLIDKIEG
                     ASLINEDSINVLEQFDNAVVLFMGAGDIQKLQNAYLDKLGMKNAF"
     gene            9764..10255
                     /locus_tag="POB55_001568"
     CDS             9764..10255
                     /locus_tag="POB55_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HDI6500532.1"
                     /translation="MDWILPIAGIIAAIAFLILCIGIVAVLNSVKKNLDYVAKTLDGV
                     EGQVQGITRETTDLLHKVNRLTEDIQGKVDRLNSVVDAVKGIGDSVQTLNSSVDRVTN
                     SITHNISQNEDKISQVVQWSNVAMEIADKWQNRHYRRGSANYKANNVATDANHSYTSR
                     VDK"
     gene            10329..11534
                     /locus_tag="POB55_001569"
     CDS             10329..11534
                     /locus_tag="POB55_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500533.1"
                     /translation="MTQQQNNKRTLKNKHTYQNEPLPNRKDFVVSFITGALVGSALGL
                     YFKNKVYQKADDLKVKEQELSQKFEERKTQLEETVAFTKERVEGFLNKSKNEQAALKA
                     QQAAIKEEVSANNLSDTSQEAQEIQEAKKEAQAETDKSAAVSNEESKAAALKAQQAAI
                     KEEASANNLSDTSQEAQEIQEAKREAQAETDKSVAVSNEESKAVALKVSKEESQAERL
                     ANAAKQKQAKLTPGSKESQLTEALFAEKPVAKNDLKEIPQLVTKKNDVSESETVNIDN
                     KDTVKQKEAKFENGVITRKADEKTTNNTAVDKKSGKQSKKTTPSNKRNASKASTNKTS
                     GQKKQHNKKSSQGAKKQSSSSKSTQKNNQTSNKNSKTTNAKSSNASKTPNAKVEKANS
                     KIEKRTFND"
     gene            11690..11773
                     /locus_tag="POB55_001570"
     CDS             11690..11773
                     /locus_tag="POB55_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500534.1"
                     /translation="MLFVTSLDYNSDIAHFKLKMEKYSIIK"
BASE COUNT         4607 a         1639 c         2199 g         3404 t
ORIGIN      
        1 gtgtattagt gtggggcgtt tcgtttttgt tcgcatttat gaatagtgat atgatgtaat
       61 aaacacatgc taattgtaat aacgaataca gaaagaatag gtgatgttgt gaaaatcggg
      121 gatatatcta ttcattatct aaatggcggc aatacaaaaa tggatggcgg tgcaatgttt
      181 ggtgttgttc cgaagccgtt gtggtcaaag caatacaatg caaatgaacg aaatcaaatc
      241 aatttaccga cacatccaat tttgattcaa acggctcaat ataatttgat tatagatgcg
      301 ggtattggta atggtaaatt atctgaaaag caattacgta attttggagt agatgaagaa
      361 agtcatatta ttactgattt ggcaaattat aatttaacgc caaaggatat tgattatgtg
      421 ctaatgacac atatgcattt tgatcatgct gccggtttga ctgatcaagc gggacatgca
      481 atttttgaaa atgcgattca tgttgtgcaa caagatgagt ggcatgagtt tattgcaact
      541 aatataagaa gtaaatcaac ttactgggat aagaataaag gcgattatag taacaagttg
      601 attttattcg aaaaacattt tgaaccggtt ccgggtatca agatgcaaca tagtggaggt
      661 catagctttg gccacacgat tattacgatt gaaagtcaag gagataaagc agttcatatg
      721 ggtgatatat tcccaactac tgcacataaa aatcctctat gggtaacggc atatgatgat
      781 tatcctatgc aatcgattcg tgaaaaagaa cgcatgatac catattttat tcagcaacaa
      841 tattggttct tgttttatca tgatgaaaac tactttgctg taaaatacag cgatgatggt
      901 gaaaacatag atgcatatat tttacgtgaa acattagttg ataataacta aaataaagat
      961 gtattactaa acaaattttc aaaaataaaa aattgagcca catccaatct tactaattag
     1021 ggtgtggctc atttttaagt tttacgatcc aaatcaaata tggataaaat tcgtattaac
     1081 gctctacaat gttaatgact tcaccagtat atgcatctgc ataaaaatca taatgaatat
     1141 tttgaccatt tttaatagtt gtaattccac cttgataaac taaacggtat ttatcagttt
     1201 caggatgaac gaatggttca taaacgatat atgagccttt gacattcata aaatatgatt
     1261 ttacttcatt aagtacttta acgggattat agcgtttacg attaatatat tggatgattg
     1321 aaatggtaga aataattaca atggctactg caattattgt tggaattata tatttcagtt
     1381 tagtcatcat ttatgctccc ttgatttcaa attcatatca ttagtttacc atattgaaga
     1441 tgatataata acttataagg agtgagaact tttatgaaca taaataaaaa agtaacatta
     1501 caacgaattc aaactttaac tgagcttcat ggggcaccag gttttgaaga agaagtaaaa
     1561 aattatatga ctcagcaaat ggcgccgtac gtagatgaat ttattgaaaa tcgtatgggt
     1621 ggattttttg gtgtgaaaaa atctaaaaat ccaaatgcaa aacgtgtaat gattgcagca
     1681 catatggatg aaatcggatt tatgattaca aatatcacta aaaatggaat gattcaattc
     1741 acaaatttag gtggtgttgc aaatgatatt tggcaaggac aacgcttagt aattaaaaat
     1801 agaaatggcg ataaaattat cggtgttgtt tctaatatac ctaaacattt tcgtactggt
     1861 agtgaaggcg caccggaaat taaagattta acattagata taggtgctca aaatgaagat
     1921 gaggtgcgtg agcgcggaat agatatagga gatacaattg tacctcacac gccattcaca
     1981 cagttatctg aacatcgata tagtgctaaa gcatgggata atcgttatgg ttgtgtcttg
     2041 gcaattgaaa tactagaatt attaaaagat atagaattag atgtagactt gtatgttggc
     2101 gcaaatgttc aagaagaggt tggattacga ggtgcgaaag catctgcaga gatgatagac
     2161 ccagacgttg catttgtagt cgattgttca cctgccaatg acgttaaagg aagccaacca
     2221 ttatctggtg aacttggtaa agggacgtta attcgcataa aagacggtac aatgatttta
     2281 aagcctgtat ttagagacta tttattaaag ttagtagaag cacatgacat tgaacatcaa
     2341 tactatatgt caccaggtgg aacagatggt ggagaaattc ataaagctaa tattggtatt
     2401 ccgactgcag ttattggtgt atgtgcacga tatattcata gtacagactc agtatttgat
     2461 ataagagact attttgcagc tagatcttta ctttcagaag ccatttgtaa tttagataat
     2521 aatcaaatag aaacattaca atataaataa tcgggtaata acaactatta tctctaaata
     2581 gttatatata ttcattaatt aaggagacat aaaaatgaaa caacttgaat cagaacaaca
     2641 atttgaatct ttaaaacaag gtgctacagt atttgaattc actgcaggct ggtgtccaga
     2701 ttgtagagtg atagaaccag atttaccgga attagaagcg agatatccta tgtttgactt
     2761 cgtatcagta gaccgtgata aatttatgga tatttgtatt gaaaatggta ttatgggtat
     2821 tccaagtttt ctagtatata aaaatggaga actgcttgga agttatattg gaaaagaacg
     2881 aaaatcaatt gaacagatag atgcattttt agctcaatac gtgtaattta gactagagaa
     2941 aaacggggta atactcgttt ttctctgtta ctatgtgttg atttattgta aactattata
     3001 aggtgcgaaa ttaggagtgt tacatatgaa tacctttcaa atgagagata aattaaagga
     3061 acgtttaagc catttagacg ttgattttaa atttaatcgt gaagaagaaa ctttgcgtat
     3121 ttatcgaaca gataataaca aaggtatcac gattaaactt aacgctatag tcgcaaaata
     3181 tgaagataaa aaagaaaaaa ttgtagatga aattgtttat tacgttgatg aagctattgc
     3241 acaaatggca gataaaaccc ttgagagtat atcatctagt caaattatgc ctgtcattag
     3301 agcgactagc tttgataaaa aaactaaaca aggtgttcct tttatctatg atgagcatac
     3361 tgcagaaaca gcagtttatt atgcagtcga tttagggaaa tcatatcgtc taattgacga
     3421 aagcatgtta gaggatttga agttaactga acaacaaata agagaaatgt ctctgtttaa
     3481 tgttagaaaa ttgtcaaatt catatacgac tgatgaagta aaaggtaata ttttttattt
     3541 tattaactca aatgacgggt atgatgcaag taggatacta aatactgcat ttttaaatga
     3601 aattgaggca caatgtcaag gcgaaatgct cgtagcagtg ccacaccaag atgtgttaat
     3661 tattgcagat atacgcaata aaacaggata tgatgtgatg gcacatttaa caatggaatt
     3721 tttcactaaa ggtctagttc caattacatc attatccttt ggatataaac agggtcatct
     3781 tgaaccgata tttattttag gtaaaaataa taaacaaaaa agagatccaa acgtgattca
     3841 gcgtttagaa gcaaatcgtc gtaaatttaa taaagataaa tagaaataat tggataagga
     3901 gttttgtcat aatgaattta ttttacaatc ctaaatatgt aggagatgtc gcatttttac
     3961 aaattgaacc agttgaaggt gaattaaact acaataaaaa aggtaatgtt gttgaaatta
     4021 ctaatgaagg taatgttgta ggttataata tttttgaaat ttcaaaagat ataacaattg
     4081 aagaaaaagg tcatattaaa ttaactgatg aacttgtaaa tgtattccaa aagcgtattt
     4141 cagaagctgg ttttgattat aaattaaatg ctgatctatc accgaaattt gtagttggct
     4201 acgttgaaac taaagacaaa catcctgatg cagataaatt aagtgtacta aatgtaaacg
     4261 ttggaaatga cacattacaa attgtatgtg gcgcgcctaa cgttgaagct ggacagaaag
     4321 ttgttgttgc taaagtaggt gcagtgatgc ctagcggtat ggtaattaaa gatgctgaat
     4381 tacgtggtgt tgcctcaagc ggtatgattt gttcaatgaa agaattgaat ttacctaatg
     4441 cacctgaaga aaaaggtatt atggtattaa atgacagcta tgaaattgga caggcatttt
     4501 ttgaataatt aaggaaggta gtgaaaatat gagctggttt gataaattat tcggcgaaga
     4561 taatgattca aatgatgact tgattcatag aaagaaaaaa agacgtcaag aatcacaaaa
     4621 tatagataac gatcatgact cattactgcc tcaaaataat gatatttata gtcgtccgag
     4681 gggaaaattc cgttttccta tgagcgtagc ttatgaaaat gaaaatgttg aacaatctgc
     4741 agatactatt tcagatgaaa aagaacaata ccatcgagac tatcgcaaac aaagccacga
     4801 ttctcgttca caaaaacgac atcgccgtag aagaaatcaa acaactgaag aacaaaatta
     4861 tagtgaacaa cgtgggaatt ctaaaatatc acagcaaagt ataaaatata aagatcattc
     4921 acattaccat acgaataagc caggtacata tgtttctgca attaatggta ttgagaagga
     4981 aacgcacaag tcaaaaacac ataatatgta ttctaataat acaaatcatc gtgctaaaga
     5041 ttcaactcca gattatcaca aagaaagttt caagacttca gaggtaccgt cagctatttt
     5101 tggcacaatg aaacctaaaa agttagaaaa tggtcgtatc cctgtaagta aaccttcaga
     5161 aaaagttgag tcagataaac aaaaatatga taaatatgta gctaagacgc aaacgtctca
     5221 aaataaacaa ttagaacaag aaaaacaaaa tgatagtgtt gtcaaacaag gaactgcatc
     5281 taaatcatct gatgaaaatg tatcatcaac aacaaaatca atgcctaatt attcaaaagt
     5341 tgataatact atcaaaattg aaaatattta tgcttcacaa attgttgaag aaattagacg
     5401 tgaacgagaa cgtaaagtgc ttcaaaagcg tcgatttaaa aaagcgttgc aacaaaagcg
     5461 tgaagaacat aaaaacgaag agcaagatgc aatacaacgt gcaattgatg aaatgtatgc
     5521 taaacaagcg gaacgctatg ttggtgatag ttcattaaat gatgatagtg acttaacaga
     5581 taatagtaca gatgctagtc agcttcatac aaatggcata gagaatgaaa ctgtatcaaa
     5641 tgatgaaaat aaacaagcgt caatacaaaa tgaagacact aatgacactc atgtagatga
     5701 aagtccatac aattatgagg aagttagttt gaatcaagta tcgacaacaa aacaattgtc
     5761 agatgatgaa gttacggttt cgaatgtaac gtctcaacat caatcagcac tacaacataa
     5821 cgttgaagta aatgataaag atgaactaaa aaatcaatcc agattaattg ctgattcaga
     5881 agaagatgga gcaacgaata aagaagaata ttcaggaagt caaatcgatg atgcagaatt
     5941 ttatgaatta aatgatacag aagcagatga ggatactact tcaaatatcg aagataatac
     6001 caatagaaac gcgtctgaaa tgcatgtaga cgctcctaaa acgcaagagc acgcagtaac
     6061 tgaatctcaa gttaataata tcgataaaac ggttgataat gaaattgaat tagcaccgcg
     6121 tcataaaaaa gatgaccaaa caaacttaaa tgtcaactca ttgaaaacga atgatgtgaa
     6181 tgatggtcat gttgtggaag attcaagcat gaatgaaata gaaaagcata acgcagaaat
     6241 tacagaaaat gtgcaaaacg aagcagctga aagtgaacaa aatgtcgaag agaaaactat
     6301 tgaaaacgta aatccaaaga aacagactga aaaggtttca actttaagta aaagaccatt
     6361 taatgttgtc atgacgccat ctgataaaaa gcgtatgatg gatcgtaaaa agcattcaaa
     6421 agtcaatgtg cctgaattaa agcctgtaca aagtaaacaa gctgtgagtg aaagcaagcc
     6481 tgcgagtcaa gccgcaccat catcaagatt tgattcacaa gagtcaaaca caaatgcata
     6541 taaaacaaat aatatgacat caaacaatgt tgagaacaat caacttattg gccatgcaga
     6601 aacagaaaat gattatcaaa atgcacaaca atattcagag cagaaacctt ctgctgattc
     6661 aactcaaacg gaaatatttg aagaaagcca agatgataat caattggaag atgagcaagt
     6721 tgatcaatca acttcgtctt cagtttcaga agtaagcgac ataactgaag aaagcgaaga
     6781 aacaacacat ccaaacaata ctagtggaca acaagataat gatgatcaac aaaaagattt
     6841 acagccttca ttttcaaatc aaaatgaaga tacagctaat gaaaatagac ctcggacgaa
     6901 ccaacaagat gttgcaacaa atcaagctgt acaaacatct aagccgatga ttcgtaaagg
     6961 cccaaatatt aaattgccaa gtgtttcatt actagaagaa ccacaagtta ttgagtcgga
     7021 cgaggactgg attacagata aaaagaaaga actgaatgac gcattatttt actttaatgt
     7081 acctgcagaa gtacaagatg taactgaagg tccaagtgtt acaagatttg aattatcagt
     7141 tgaaaaaggt gttaaagttt caagaattac ggcattacaa gatgacatta aaatggcatt
     7201 ggcagcgaaa gatattcgta tagaagcgcc tattccagga actagtcgtg ttggtattga
     7261 agttccgaac caaaatccaa cgacagtcaa cttacgttct attattgaat ctccaagttt
     7321 taaaaatgct gaatctaaat taacagttgc gatggggtat agaattaata atgaaccatt
     7381 acttatggat attgctaaaa cgccacacgc actaattgca ggtgcaactg gatcagggaa
     7441 atcagtttgt atcaatagta ttttgatgtc tttactatat aaaaatcacc ctgaggaatt
     7501 aagattatta cttatcgatc caaaaatggt tgaattagct ccttataatg gtttgccaca
     7561 tttagttgca ccggtaatta cagatgtcaa agcagctaca cagagtttaa aatgggccgt
     7621 agaagaaatg gaacgacgtt ataagttatt tgcacattac catgtacgca atataacagc
     7681 atttaacaaa aaagcaccat atgatgaaag aatgccaaaa attgtcattg taattgatga
     7741 gttggctgat ttaatgatga tggctccgca agaagttgag cagtctattg ctagaattgc
     7801 tcaaaaagca agagcatgtg gtattcatat gttagtagct acgcaaagac catctgtcaa
     7861 tgtaattaca ggtttaatta aagccaacat accaacaaga attgcattta tggtatcatc
     7921 aagtgtagat tcgagaacga tattagacag tggtggagca gaacgcttgt taggatatgg
     7981 cgatatgtta tatcttggta gcggtatgaa taaaccgatt agagttcaag gtacatttgt
     8041 ttctgatgac gaaattgatg atgttgttga ttttatcaaa caacaaagag aaccggacta
     8101 tctatttgaa gaaaaagaat tgttgaaaaa aacacaaaca caatcacaag atgaattatt
     8161 tgatgatgtt tgtgcattta tggttaatga aggacatatt tcaacatcat taatccaaag
     8221 acatttccaa attggctata atagagcagc aagaattatc gatcaattag agcaactcgg
     8281 ttatgtttcg agtgctaatg gttcaaaacc aagggatgtt tatgttacgg aagcagattt
     8341 aaataaagaa taattatgag taaggagttt tatataatga cacactatca ttttgtcgga
     8401 attaaaggtt ctggcatgag ttcattagca caaatcatgc atgatttagg acatgaagtt
     8461 caaggatcgg atattgagaa ctacgtattt acagaagttg ctcttagaaa taaggggata
     8521 aaaatattac catttgatgc taataacata aaagaagata tggtagttat acaaggtaat
     8581 gcattcgcga gtagccatga agaaatagta cgtgcacatc aattgaaatt agatgttgta
     8641 agttataatg attttttagg acagattatt gatcaatata cttcagtagc tgtaactggt
     8701 gcacatggta aaacttctac aacaggttta ttatcacatg ttatgaatgg tgataaaaag
     8761 acttcatttt taattggtga tggcacaggt atgggattgc ctgaaagtga ttatttcgct
     8821 tttgaggcat gtgaatatag acgtcacttt ttaagttata aacctgatta cgcaattatg
     8881 acaaatattg atttcgatca tcctgattat tttaaagata ttaatgatgt ttttgatgca
     8941 ttccaagaaa tggcacataa tgttaaaaaa ggtattattg cttggggtga tgatgaacat
     9001 ctacgtaaaa ttgaagcaga tgttccaatt tattactatg gatttaaaga ttcggatgac
     9061 atttatgctc aaaatattca aattacggat aaaggtactg cttttgatgt gtatgtggat
     9121 ggtgagtttt atgatcactt cctgtctcca caatatggtg accatacagt tttaaatgca
     9181 ttagctgtaa ttgcgattag ttatttagag aagctagatg ttacaaatat taaagaagca
     9241 ttagaaacgt ttggtggtgt taaacgtcgt ttcaatgaaa ctacaattgc aaatcaagtt
     9301 attgtagatg attatgcaca ccatccaaga gaaattagtg ctacaattga aacagcacga
     9361 aagaaatatc cacataaaga agttgttgca gtatttcaac cacacacttt ctctagaaca
     9421 caagcatttt taaatgaatt tgcagaaagt ttaagtaaag cagatcgtgt attcttatgt
     9481 gaaatttttg gatcaattag agaaaatact ggcgcattaa cgatacaaga tttaattgat
     9541 aaaattgaag gtgcatcgtt aattaatgaa gattctatta atgtattaga acaatttgat
     9601 aatgctgttg ttttatttat gggtgcaggt gatattcaaa aattacaaaa tgcatattta
     9661 gataaattag gcatgaaaaa tgcgttttaa tatgtttata atagagtagt atgggtattt
     9721 attattaatg acattattac atgttaatta ggaggcgttt ttaatggatt ggattttacc
     9781 aattgctgga attatcgctg cgattgcatt cttaatttta tgtatcggta tcgtagctgt
     9841 attaaattct gttaagaaaa acttagatta tgttgcaaaa acacttgacg gtgtagaagg
     9901 tcaagttcaa ggtattactc gtgaaacaac agatttactt cataaagtaa accgtttaac
     9961 tgaggatatc caaggtaaag tagatcgttt aaactcagtt gtagatgctg ttaaaggtat
    10021 cggtgactca gtacaaacgt taaacagctc tgtagatcgt gtaacaaatt caattacaca
    10081 taatatttct caaaatgaag ataaaatctc acaagttgtt caatggtcaa atgttgcaat
    10141 ggaaattgca gacaaatggc aaaatagaca ctaccgtcgt ggaagtgcaa attacaaagc
    10201 taataatgta gcaactgatg caaatcatag ctatacttct cgagtagata aataattaat
    10261 aattaaacaa atgcacttaa atgaggttgt tacttattta agtgcatttg ttataggagg
    10321 atacacaaat gacgcaacaa caaaataata aaagaacatt aaaaaataaa cacacttatc
    10381 aaaatgaacc attacccaac cgtaaagatt ttgttgttag ttttataact ggcgcgcttg
    10441 ttggttcagc tttaggtttg tattttaaaa ataaagttta tcaaaaagca gatgatttaa
    10501 aagtcaaaga acaagaactg tcgcaaaaat ttgaagaaag aaaaacgcaa cttgaagaaa
    10561 cggttgcctt tacaaaagaa cgtgttgaag gatttttaaa caaatctaaa aatgagcaag
    10621 cagcattaaa agcacaacaa gcagcaataa aagaagaagt aagtgcaaat aatttgagtg
    10681 atacatcaca agaggcacaa gagattcaag aagctaaaaa agaagcacaa gcagaaacag
    10741 ataaaagtgc agctgtatca aatgaagaat caaaagcagc ggcattaaaa gcacaacaag
    10801 cagcaataaa agaagaagca agtgcaaata atttaagtga tacatcacaa gaggcacaag
    10861 agattcaaga agctaaaaga gaagcacaag cagaaacaga taaaagtgta gctgtatcaa
    10921 atgaagaatc aaaagcagtg gcattaaagg tatcgaaaga agagtcacaa gctgaaagat
    10981 tagcaaacgc tgcaaaacag aagcaagcta aattaacacc aggttcaaaa gagagtcaat
    11041 taactgaagc gttatttgca gaaaaaccag ttgctaaaaa tgacttgaaa gaaattcctc
    11101 aattagttac taaaaagaat gatgtatcag agtcagagac agttaatata gataataaag
    11161 acactgttaa acaaaaagaa gctaaatttg aaaatggtgt tattacacgt aaagctgatg
    11221 aaaaaacaac taataataca gctgttgaca agaaatcagg taaacaatct aaaaaaacaa
    11281 caccttcaaa taaacgaaat gcatcaaaag catctacaaa taaaacttca ggtcagaaaa
    11341 agcaacataa taagaaatca tcacaaggtg caaagaaaca aagtagttca agtaagtcaa
    11401 ctcaaaagaa taatcaaact agtaataaga attcaaaaac aacaaatgct aaatcatcca
    11461 atgcatcaaa aacgccaaat gctaaagttg aaaaagctaa tagtaaaata gagaaacgta
    11521 cattcaatga ctaagattac taaaatcgtt taataatcaa tcatagcagt aagctatgta
    11581 ttatattttt aaaaattgaa tttcaaaata aaaggtaggc attcaagtgc ttaccttttt
    11641 tgatatcatc agtttatttt gaaaggcaac gcgatcattc catgtattta tgttgtttgt
    11701 aacatcgtta gattacaata gtgatattgc acattttaag ctgaagatgg aaaaatattc
    11761 gataatcaaa taaacaatga atcttagaag gtacaatgac gttcactaat ttaaatatag
    11821 ctgaatgtgt tggtgagtga tgttcacta
//
LOCUS       DAMZKC010000080        11824 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.080, whole genome shotgun sequence.
ACCESSION   DAMZKC010000080 DAMZKC010000000
VERSION     DAMZKC010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11824
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.080"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            138..1358
                     /locus_tag="POB55_001571"
     CDS             138..1358
                     /locus_tag="POB55_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HDI6500535.1"
                     /translation="MDMFAALLQIKNYKLFVANMFLLGMGIAVTVPYLVLFATKDLGM
                     TTNQYGLLLASAAISQFTVNSIIARFSDTHHFNRKIIIILALLMGALGFSIYFFVDTI
                     WLFILLYAIFQGLFAPAMPQLYASARESINVSSSKDRAQFANTVLRSMFSLGFLFGPF
                     IGAQLIGLKGYAGLFGGTISIILFTLVLQVFFYKDLNIKHPISTQQHVEKIAPNMFKD
                     KTLLLPFIAFILLHIGQWMYTMNMPLFVTDYLKENEQHVGYLASLCAGLEVPFMIILG
                     VLSSRLQTRTLLIYGAIFGGLFYFSIGVFKNFYMMLAGQVFLAIFLAVLLGIGISYFQ
                     DILPDFPGYASTLFSNAMVIGQLGGNLLGGAMSHWVGLENVFFVSAASIMLGMILIFF
                     TKNQKITKEDVIST"
     gene            1355..1843
                     /locus_tag="POB55_001572"
     CDS             1355..1843
                     /locus_tag="POB55_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HDI6500536.1"
                     /translation="MTIILWLLIIAAFMLAFVGLIKPIIPSVLVLWVGFLIYQFGFHN
                     QHLSWVFYVSMALLTILILCADFLANKYFVNRFGGSKFGEYAALIGVVIGCFVLPPFG
                     IIIIPFILVFIVELIQGYSFERAVKVSIGSIVAFLTSSIAQAIIMFIMIVWFFIDALL
                     IN"
     gene            complement(1942..2631)
                     /locus_tag="POB55_001573"
     CDS             complement(1942..2631)
                     /locus_tag="POB55_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 family protein"
                     /protein_id="HDI6500537.1"
                     /translation="MKSTAQLTKENNVKSLRLSNTDREIFENYMTYMRSDFRVNPHDT
                     ELIINRILKQLLSAEQHGLLALDFFNHDPKAHAIKELKAMPNETFKNIFKYIYQHIVL
                     LIGIVSFLKGFLGFFMEKNGSNLYFVSFPFSVVVGFFIVFLFIWFSFKTIQLQCFNNS
                     NWIWIFTYLAIILLIVGFFYVFFIPQSLLAFGPYIQVSNWVFIIFSFIVMPIGLRIER
                     NISKKHSHTFL"
     gene            2758..3201
                     /locus_tag="POB55_001574"
     CDS             2758..3201
                     /locus_tag="POB55_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6500538.1"
                     /translation="MRTLNKDEHNYIKQIANIHETLLSQVESNYKCTKLSIALRYEMI
                     CSRLEHTNDKIYIYENEGQLIAFIWGHFSNEKSMVNIELLYVEPQFRKLGIATQLKIA
                     LEKWAKTMNAKRISNTIHKNNLPMISLNKDLGYQVSHVKMYKDID"
     gene            3268..3663
                     /locus_tag="POB55_001575"
     CDS             3268..3663
                     /locus_tag="POB55_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500539.1"
                     /translation="MKKLIISIMAVMLFLTGCGKSQEKATLEKDIDNLQKENKELKDK
                     KEKLQQEKEKLADKQKDLEKEVKDLKPSKEDNKDDKKDEDKNKDKDKDKEASQDKQSK
                     DQTKSSDKDNHKKPTSADKDQKANDKHQS"
     gene            3801..4100
                     /locus_tag="POB55_001576"
     CDS             3801..4100
                     /locus_tag="POB55_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500540.1"
                     /translation="MHEQDFRILEGQDITLPELGRELENITGHTIADSTGEIKRVIAH
                     LPNFESDTDTFVATYRLNHQQDFIDATFTALKSDRARLKEVPVHVELISYISKSK"
     gene            4180..4722
                     /locus_tag="POB55_001577"
     CDS             4180..4722
                     /locus_tag="POB55_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6500541.1"
                     /translation="MIYCETERLILRDWHEDDLLPFQKMNANYDVRKYFPSLLSYRRS
                     ELDMRTMDAVIKDYGIGLFAVEDKESHQWIGFIGLNYIPETSDYPFKELPLYEIGWRL
                     LPEFWGKGLATEGAKATLKLAEEHQIYDVYSFTAEANKASQRVMEKIGMTVYDHFELP
                     NLSKYHLLKRQVRYYINLRK"
     gene            complement(4818..5384)
                     /locus_tag="POB55_001578"
     CDS             complement(4818..5384)
                     /locus_tag="POB55_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HDI6500542.1"
                     /translation="MKRIAVYCGASKGHDPSYVQKAYDLGKYFAEQGYELVFGAGSIG
                     IMGAIQDGVLDHGGKAIGVMPKMLDEHEITSQRLTELILVDSMHERKNKMAELADAFV
                     MAPGGAGSLEEFFEMYSWAQIGIHEKPIAIYNINGFFNPLQTMIDHMIEEGFIDPKYR
                     ALAPLCDTKESLIESILNFKPLGTRSYD"
     gene            complement(5386..5844)
                     /locus_tag="POB55_001579"
     CDS             complement(5386..5844)
                     /locus_tag="POB55_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HDI6500543.1"
                     /translation="MTHIIIDGDACPVVDSIIDLTTETGIFVTIIRSFSHFSNQLYPP
                     HVSTLYVDDGPDAVDYKIVQLSTKDDIVVTQDYGLASLLVDKVLIVMHHNGKIYNSKN
                     IQQLLDKRYMNAQIRKQGGRHKGPPPFTKQDQKVFEQSLLKVIHRIKELD"
     gene            complement(5847..6530)
                     /locus_tag="POB55_001580"
     CDS             complement(5847..6530)
                     /locus_tag="POB55_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500544.1"
                     /translation="MDKKKVIKFMINVLPIVLVPLIVERKRIKQHPDVQKVTDATSKV
                     ASKTSAAISNTASDVKEYVGDKKQDFENKRELKKFAREHDPAYIEKKGEKLAKQNRKD
                     ADKMNKILQKNIEKRHKEEQKAREKNEIQRIKDMKKSQKYEEKAGLTPNKLDEKTEKK
                     GDKLAEKNRKEIAKMNKKLQKNIEKRHKEEQKRQQEADKARIKSFKKYKDYVAKSASQ
                     QNKENNTEA"
     gene            complement(6702..7577)
                     /locus_tag="POB55_001581"
     CDS             complement(6702..7577)
                     /locus_tag="POB55_001581"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDI6500545.1"
                     /translation="MFIIELIKGIILGVVEGLTEFAPVSSTGHMILVDDMWLKSSEFL
                     GSQSAFTFKIVIQLGSVFAAAWVFRERFLEILHIGKHKHVEGDNNQQRRSKPRRLNLL
                     HVLVGMVPAGILGLLFDDFIEEHLFSVPTVMIGLFVGAIYMIIADKYSAKVKNPQTVD
                     QINYFQAFVIGISQAVAMWPGFSRSGSTISTGVLMKLNHKAASDFTFIMAVPIMLAAS
                     GLSLLKHYQDIQITDIPFYILGFLAAFTVGLIAIKTFLHLINKIKLIPFAIYRIVLVI
                     FIAILYFGFGIGKGI"
     gene            7796..9427
                     /locus_tag="POB55_001582"
     CDS             7796..9427
                     /locus_tag="POB55_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HDI6500546.1"
                     /translation="MKKLTIILFQYKIFPVLMFLVSTGLGILVITQNILIADFLAKII
                     RHQFQGLWIVLFILLGVLLLRATVQFLNQWLGDTLAFKVKHMLRQRVIYKNNGHPIGE
                     QMTILTENIDGLAPFYKSYLPQVFKSMMVPLIIIIAMFFIHFNTALIMLITAPFIPLF
                     YIIFGLKTRDESKDQMTYLNQFSQRFLNIAKGLVTLKLFNRTEQTEKHIYDDSTQFRT
                     LTMRILRSAFLSGLMLEFISMLGIGLVALEATLSLVVFHNIDFKTAAIAIILAPEFYN
                     AIKDLGQAFHTGKQSEGASDVVFEFLEQPNNNNEFLLKYEENQKPFIQLTDISFRYDN
                     SDRLVLNDLNLEIYNGDQIALVGPSGAGKSTLTHLIAGVYQPTIGTISTNQRDLNIGI
                     LSQQPYIFSASIKENITMFKDIENNTIEEVLDEVGLLDKVQSFTKGINTIIGEGGEML
                     SGGQMRRIELCRLLVMKPDLVIFDEPATGLDIQTEHMIQNVLFQHFKDTTMIVIAHRD
                     NTIRHLQRRLYIENGRLIADDRNISVNITENGDDL"
     gene            9424..11097
                     /locus_tag="POB55_001583"
     CDS             9424..11097
                     /locus_tag="POB55_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding/permease
                     protein"
                     /protein_id="HDI6500547.1"
                     /translation="MKTRLKFQVDKDLLLAIVVGVCGSLVALAMFFLSGYMVTQSALG
                     APLYALMILVVTVKLFGFLRAITRYVERLISHKATFTMLRDIRVQFFGKLVNVIPNVY
                     RKLSSSDLISRMISRVEALQNIYLRVYYPPVVIGLTALVTVIVLAFISIGHALLIMVS
                     MLFTLLVVPWLSSKKARTLKKHAANEQARFLNHFYDYKAGMDELRRFNQINHYRDNLM
                     AKLNHFDKLQLKEQRFLTIYDFILNIIAMLSIFGSLVLGLIQINAGQLNIIYMTSIVL
                     MVLTLFEQAVPMTNVAYYKADTDQALHDINEVISVPSTNGKNRLNDKYDATNIYEVKD
                     ASFKYWNQQTYVLSDINFNVNRGEKIAIVGPSGSGKSTLLQIMAGLYQLDSGSIRFEN
                     MDMFEIDDKDKFESLNVLLQSQQLFDGTIRQNLFTDEKDEAVQAIFKQLDLEHLALER
                     QIDLDGHTLSGGEIQRLAIARMLLKDTASTWILDEPTTALDKQNSLKVMDLIEAHAET
                     LIVATHDLTLLSRFETIIVMINGKIVEKGNYQQLLANQGALWNMIQYNA"
     gene            complement(11224..11667)
                     /gene="mgrA"
                     /locus_tag="POB55_001584"
     CDS             complement(11224..11667)
                     /gene="mgrA"
                     /locus_tag="POB55_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MgrA"
                     /protein_id="HDI6500548.1"
                     /translation="MSDQHNLKEQLCFSLYNAQRQVNRYYSNKVFKKYNLTYPQFLVL
                     TILWDESPVNVKKVVTELALDTGTVSPLLKRMEQVDLIKRERSEVDQREVFIHLTDKS
                     ETIRPELSNASDKVASASSLSQDEVKELNRLLGKVIHAFDETKEK"
BASE COUNT         3977 a         1718 c         2003 g         4126 t
ORIGIN      
        1 ttgctaatgc accaactgca taagagcccc taattaataa attaaaaggg gacaaaggaa
       61 tacagttgga tgctaacgca ccaactgcat aagagcccct aattaataaa ttaaaagggg
      121 ctctaagaag cggggttatg gatatgtttg cagcgttatt acaaataaag aattataaac
      181 tctttgttgc taatatgttt ctactaggta tgggtattgc ggttacggtc ccatatcttg
      241 ttctttttgc aactaaagat ttaggtatga caacaaatca gtatggatta cttctagcat
      301 ctgcagcgat tagccagttt acagtaaatt caattattgc tagattttcg gatacgcatc
      361 actttaatag aaaaattatt attattctcg cattattaat gggtgcgctt ggtttttcaa
      421 tatacttttt tgtagataca atctggttat tcatattact atatgcgatt ttccaaggat
      481 tatttgcacc agcaatgccc caactttacg catctgctag agaatctatc aatgtttcaa
      541 gctctaaaga tagagctcaa tttgccaaca cagtattacg ttcaatgttc tcattgggct
      601 ttttatttgg tccatttatt ggtgcccaat taatcggttt aaaaggctat gctggattgt
      661 ttggtggaac aataagtatc attttattta ctttagtact tcaagtgttt ttctataagg
      721 atttaaacat taaacaccct attagtacgc aacaacatgt tgaaaaaatt gctcctaata
      781 tgtttaaaga caaaacgctt ttattaccat ttattgcatt tattttatta cacattggac
      841 aatggatgta tacgatgaat atgcctttat ttgttactga ttatttaaaa gaaaatgaac
      901 aacatgtcgg ttatttagct agtttatgtg ctggtttaga agtgccattt atgatcattc
      961 ttggcgtttt atcatctaga ttacagactc gaacattgtt gatttatgga gcgatttttg
     1021 gtggtttatt ctacttcagc attggggtat ttaaaaactt ctatatgatg ttagcaggac
     1081 aggtgttttt agctattttc ttagcggttc ttttaggaat tggtattagt tatttccaag
     1141 atatcttacc agattttcca ggatacgcct caacactatt ttctaatgca atggttattg
     1201 gacagttagg cggtaaccta ttaggtggtg ctatgagtca ctgggtaggt ttggaaaatg
     1261 tattttttgt atcagcagca tcaatcatgt taggtatgat acttatattc tttactaaaa
     1321 atcaaaaaat tacaaaagag gatgtgatat caacatgaca attattttat ggctacttat
     1381 catcgctgcc ttcatgttag catttgttgg gttgattaag ccgattattc cttctgtttt
     1441 agtattatgg gttggctttt taatctatca atttggcttt cataatcagc atttatcatg
     1501 ggtgttttat gtatctatgg cattgctaac aatattaatt ttatgtgccg actttttagc
     1561 taataaatat tttgtgaatc gcttcggtgg ttctaagttt ggagagtatg cagctttaat
     1621 tggtgtggtt attggatgtt ttgttttacc gccatttgga attattatta taccttttat
     1681 tttggtattc atagttgaat taatacaagg ctattcattt gaaagagcag ttaaagtaag
     1741 tataggttca atcgtagcat ttttaacaag tagtatagct caagcaatca ttatgtttat
     1801 aatgattgta tggttcttta tagatgcttt attgattaat taataaaaag cttattgcaa
     1861 aatatgtttt tcggtaactg taatttagtg attttatcat taacagtacc aaattcgcat
     1921 atgatgtaat aagctttttg tttataaaaa tgtatgagaa tgttttttcg aaatatttct
     1981 ttcaatgcgt aatccaatag gcataactat aaatgaaaat ataataaata cccaattgct
     2041 tacctgaata tatggtccaa aggcaagtaa tgattgtgga ataaagaata catagaaaaa
     2101 tcctacaatt agtagaatga ttgctaaata agtgaatatc catatccaat ttgaattgtt
     2161 aaaacactgc agttgtattg ttttaaaact aaaccatata aataaaaaga caataaagaa
     2221 tccaacgact actgaaaacg ggaatgaaac aaaatataaa ttacttccat ttttttccat
     2281 gaaaaatcct aaaaatcctt tgagaaaact aacaatccca attaatagaa cgatgtgttg
     2341 atagatatat ttaaaaatat ttttaaatgt ttcattaggc atcgctttta gttcttttat
     2401 tgcatgtgct tttgggtcgt gattgaaaaa atctaaggct aataaaccat gttgttctgc
     2461 gcttaataat tgtttgagta tacggttaat aattaactct gtatcatgag ggttgacgcg
     2521 aaagtcagag cgcatataag tcatataatt ctcgaagatt tctctatcag tattgcttaa
     2581 tcttaatgat ttaacattat tttcttttgt taattgcgca gtacttttca ttgttactta
     2641 agcgctcctt taaaaatgtt taattccaaa ttaaaatgga aatgatttta tagtattaat
     2701 aaggtcaatc atatcatatt aaacgcataa atataacgat taatattgga gaggaaaatg
     2761 aggacactta ataaagatga acataattat atcaagcaaa tagctaatat acatgagaca
     2821 ttattgtcgc aagtagaatc caactataaa tgtactaaac tgagtattgc tcttaggtac
     2881 gagatgatat gttcaagatt agaacataca aatgataaaa tttatatata tgaaaatgaa
     2941 ggtcaattaa tagcgtttat ttggggacat tttagtaatg aaaaaagtat ggttaacatt
     3001 gaactgctat atgttgaacc acaatttcgc aaactgggaa tagctacgca actgaagatt
     3061 gcgcttgaaa aatgggcaaa aactatgaat gcaaagcgaa taagcaatac aattcataaa
     3121 aataatttgc caatgatatc tttgaataaa gatttaggtt atcaagtgag tcatgtgaaa
     3181 atgtataaag atattgatta gaattaggat tatgttgcta attcatgtta aaattaaaaa
     3241 agatttaatg acgttaagga gttttatatg aagaaattaa tcatcagtat tatggcggtc
     3301 atgctatttt taacaggttg tggtaaaagt caagagaaag ccactctgga aaaggatatc
     3361 gataatttac aaaaagaaaa taaagaatta aaagacaaaa aagaaaagct tcaacaagaa
     3421 aaagaaaaat tagcagataa gcaaaaagac cttgaaaaag aagtgaaaga tttaaaacct
     3481 tcaaaagaag ataacaagga tgataaaaaa gacgaagaca aaaataaaga caaagataaa
     3541 gataaagagg catcacaaga taagcaatca aaagatcaaa ctaagtcatc ggataaagat
     3601 aatcacaaaa agcctacatc agcagataaa gatcaaaaag ctaatgacaa acaccaatca
     3661 taatcgaatt gcttacttgt tatagatgaa aggtacagcg ttttaaacct tattttaagg
     3721 gtatgtatta attaaaatgt ggtcatgatt gaaacagaac gtaaaaatag acaacgtaat
     3781 taataaagga gagaaacggc atgcatgaac aagattttag aattttagag ggtcaagata
     3841 ttactttgcc agaattaggt agagaattag aaaatattac aggacatacg attgctgatt
     3901 ctactggcga aattaagcgt gtaattgcac atttaccaaa ctttgagtcc gatacagata
     3961 cttttgttgc tacatatcgt ttaaaccatc aacaagattt tatagatgca acttttactg
     4021 cgctgaaatc agatagagca cgtttaaaag aagtgccagt tcatgttgaa cttataagtt
     4081 atatttctaa atcaaaataa actgctatct aaaacgcaaa gttgatcaaa atatcgattt
     4141 tgtgtttttt attgagaaat tatataggag tgtcaatcga tgatttattg tgaaacagag
     4201 cgtttaatat taagagactg gcatgaagat gatctgttac cttttcaaaa aatgaatgcg
     4261 aattatgacg tacgtaaata ttttccaagt ttattgagtt atcgtcgttc agaattagat
     4321 atgagaacta tggatgcggt tattaaagat tatggcattg gattatttgc tgtagaagat
     4381 aaagagtccc atcaatggat aggctttata ggtttgaatt atattccaga aacaagcgat
     4441 tatccattta aagaattacc gctttatgaa ataggttggc gcttgttgcc agaattttgg
     4501 ggaaaaggat tagcaactga aggcgcaaag gcaacattga agttagcaga agaacatcaa
     4561 atatacgatg tctatagttt tacagcagaa gcaaataaag cttcacaacg tgtaatggaa
     4621 aaaattggca tgacagtgta tgatcatttc gaattaccca atctaagtaa gtatcattta
     4681 ttaaaaaggc aagtgcgcta ttacattaat cttcgaaagt gaaaaattta tacataagcg
     4741 taacaaacac ccctaacatt gtttagctga tgataacaat attaggggtg tttgtttatt
     4801 ttttaacctt agaatgatta atcgtatgaa cgagtaccca gaggtttgaa atttaatatt
     4861 gattcaatta atgattcctt agtgtcgcat aacggtgcaa gagcacgata cttaggatca
     4921 ataaaacctt cctcaatcat atggtcaatc attgtttgta gtggattgaa aaagccatta
     4981 atattataaa tggcaatagg cttttcatgg atacctattt gagcccaact atacatttcg
     5041 aaaaattctt ctagtgaacc tgcgccacca ggagccatga caaatgcatc tgcaagttct
     5101 gccattttat ttttacgttc atgcatagaa tcaactaaaa ttaattcagt taaacgttgg
     5161 cttgtgattt catgttcatc taacatttta ggcatgacgc caatagcttt gccgccatga
     5221 tctaatacac catcttgaat ggcacccata atgccaattg accctgcacc aaatactaat
     5281 tcataacctt gttcagcaaa atatttacct aaatcgtatg ctttttgtac atatgaaggg
     5341 tcatgacctt tgcttgcacc acaataaact gcgattcgtt tcatgttaat ccagctcctt
     5401 aattcgatga atgactttta atagtgattg ttcaaacact ttttgatctt gctttgtaaa
     5461 aggtggggga cctttgtggc gaccaccttg ttttctaatt tgtgcattca tatatcgttt
     5521 atctaatagt tgttgaatat ttttggaatt gtatatcttc ccattatgat gcatgacaat
     5581 taagactttg tcgactaata aacttgcgag tccataatct tgagtgacta cgatatcatc
     5641 cttcgttgat aattgaacaa ttttgtaatc aactgcatct ggtccatcat caacatataa
     5701 tgttgataca tgtggaggat ataattggtt cgaaaaatgg ctgaagctcc gaataattgt
     5761 cacaaaaatg cctgtctcag ttgttaaatc tataatagaa tcaacaacag gacaagcatc
     5821 tccatcaata ataatatgtg tcacaattat gcctctgtat tgttttcttt attttgttga
     5881 gaggcgcttt tggcaacata atctttatat tttttaaatg acttgatgcg tgctttatca
     5941 gcttcttgtt ggcgtttttg ttcttctttg tgtcgttttt caatattttt ttgtaacttt
     6001 ttattcattt tagcgatttc tttgcgattt ttttcagcta gtttatcgcc ttttttctca
     6061 gttttctcat ctaatttatt aggtgttaag cctgcttttt cttcgtattt ttgtgatttt
     6121 ttcatatctt taatacgttg tatttcattc ttttcgcggg ctttttgctc ttctttatga
     6181 cgcttttcga tatttttttg aagtatttta ttcattttat cagcgtcttt acgattttgt
     6241 ttagctaatt tttcgccttt tttctcaata taggcaggat catgttctct agcaaacttt
     6301 ttaagttcac gtttattttc aaaatcttgt tttttatcgc cgacatattc tttaacatca
     6361 ctcgctgtgt tactgattgc tgcagatgtt tttgaagcaa ctttacttgt agcatctgta
     6421 actttttgta cgtccggatg ttgtttgata cgtttacgtt caacaattaa cggtaccaat
     6481 acaattggta atacattaat cataaatttg atgacttttt tcttatccat agatcttgcc
     6541 tccataatta ctttattaat tttacatacc ctatgataca tcaatataaa cgatgatagt
     6601 agtgaatcac tattaagtat ttcagatctt ttttaaaaga agacaataaa aactgccaat
     6661 caagtggttc cttaattgac agtctatatt ttaaaaggaa attaaatacc tttaccaatg
     6721 ccaaatccga agtaaagtat agcaataaag attactaata caattctgta aatggcaaat
     6781 ggaattaatt tgattttgtt aattagatgc aagaatgttt tgattgcaat tagtccaaca
     6841 gtaaatgcag ctaaaaagcc taaaatataa aaaggtatat cagtaatctg aatatcttga
     6901 taatgtttta ataaagataa accactagct gctaacataa ttggaacagc cataataaat
     6961 gtgaagtccg atgctgcttt atgatttaat ttcattaata ccccagttga aattgttgag
     7021 cctgaacggc tgaaaccagg ccacatagct actgcttgag agataccaat tacaaacgct
     7081 tggaaataat tgatttgatc cactgtttgt gggtttttaa ctttagctga gtatttatca
     7141 gcaataatca tatagatagc acctacgaat aagccaatca taacagttgg cacactaaat
     7201 aaatgttctt cgatgaaatc atcaaatagt aagcctaaaa tacctgctgg aaccataccc
     7261 actaatacat gtaataaatt taaacgtctt ggctttgaac gtctttgttg attgttatct
     7321 ccttcaacat gtttgtgttt accaatatgt aaaatctcta agaagcgttc gcggaacacc
     7381 catgctgctg caaagacgga tcctaattgg atgacgattt taaatgtaaa tgctgattga
     7441 gaacctaaaa attcagatga ttttaaccac atatcatcaa ctagaatcat atgtccagta
     7501 gaggaaacag gtgcaaattc tgttaatcct tcgacgaccc ctaagataat accttttatt
     7561 aattcaatga taaacataat gtacccactt tcattactca atttaattta tttaaatatc
     7621 aaaattacca tatcatgata gcatattcat ttaaagacat gctagttata gttataatac
     7681 tagactaaag atgtatatat tcattttctt ttacatgtaa aactacaata ttttattgag
     7741 ctatttaatt tgattttaag gaaaaccttt tataataggt ttaggtgata taattgtgaa
     7801 aaaattaaca ataatactgt ttcaatataa aatttttccg gtactcatgt tcttggtcag
     7861 tactggtctc ggcatactcg ttataacgca aaatatttta atagcagatt ttttagctaa
     7921 aattataaga catcaatttc aaggtttatg gattgtatta tttattttat taggtgtttt
     7981 acttttaaga gcaactgtgc aatttctaaa tcaatggtta ggtgatacat tagcatttaa
     8041 agttaagcat atgcttagac agcgggttat ttataaaaat aatggtcatc caatcggtga
     8101 acaaatgact atactcacag aaaacattga tggtttagca cctttttata agagttattt
     8161 gcctcaagtg ttcaaatcaa tgatggttcc gctcatcata atcattgcaa tgtttttcat
     8221 ccatttcaat accgcattaa ttatgttaat aactgcacca tttattcctt tgttttatat
     8281 tattttcggt ttgaaaacgc gagatgagtc aaaagatcaa atgacttatt tgaatcaatt
     8341 tagtcaacgg tttttaaata ttgctaaagg tttagtgacg ttaaagctat ttaatcgtac
     8401 agagcaaaca gagaagcata tttacgacga tagtactcag tttagaactt taacaatgcg
     8461 cattttacgc agtgcttttt tatcgggatt aatgctcgaa tttataagta tgttaggtat
     8521 tggattggtt gcattggaag caacgctaag cttagtagta tttcataata ttgattttaa
     8581 aactgcggca attgcgatta ttttagcgcc tgaattttat aatgcaatta aggacttagg
     8641 gcaagcgttc catactggaa aacaaagtga aggtgccagt gacgttgtgt ttgagttttt
     8701 agaacaaccg aacaataata atgaatttct attaaagtat gaggaaaatc aaaagccatt
     8761 tattcagtta acagacatat catttcgata tgataattct gatagattgg tattaaatga
     8821 tttaaatttg gaaatatata atggtgatca aattgcactt gtaggtccaa gcggggcagg
     8881 taaatccact ttgacacatc taattgcagg tgtttatcag ccaacaatag gtactataag
     8941 tacaaaccag cgtgatttaa atataggaat acttagtcaa cagccatata ttttcagtgc
     9001 ttctataaaa gagaatatta cgatgtttaa agatatagaa aataatacta ttgaagaagt
     9061 gctagacgaa gtaggtttat tagacaaagt gcaatctttc acaaaaggca ttaacacaat
     9121 aataggtgaa ggaggcgaaa tgttatctgg tggacagatg agacgcatag aactttgccg
     9181 tcttttagtt atgaagccag atctcgttat atttgatgag cctgcaactg gtttagatat
     9241 tcaaacagaa cacatgattc agaacgttct gtttcaacat tttaaagata caacgatgat
     9301 tgtcattgca catagagata atacaattcg ccatttacaa cgacgcttgt atatagaaaa
     9361 tggaagactg attgctgatg atcgcaatat ttcagtaaat ataacagaaa atggtgatga
     9421 cttatgaaaa cacgactaaa atttcaagta gataaggatt tattgttagc tatagttgtt
     9481 ggtgtttgtg gaagtttagt tgcgctcgcc atgtttttct taagtggtta tatggtgaca
     9541 caaagtgcac ttggtgcgcc actatacgct ctgatgattt tagtcgttac agtaaaattg
     9601 tttgggtttt taagagctat tactcgatac gtagagcgcc ttatttctca taaagctaca
     9661 tttacaatgc tacgtgatat tcgggtacag tttttcggta aattagtaaa tgtcattcct
     9721 aatgtttacc gtaaactgag ttctagtgat ttaatttcac gtatgattag tcgtgttgag
     9781 gcattacaaa atatatattt acgtgtttat tatccaccag tcgtcatcgg tttgacagcg
     9841 ctagttacag tcatagtttt ggcgttcatt tcaatcggcc atgcgctatt gattatggtt
     9901 agcatgttgt ttactttact cgttgttcct tggttaagct caaaaaaagc acgtacttta
     9961 aagaaacatg cagctaatga acaggcccga tttttaaatc atttttatga ttataaagct
    10021 ggtatggatg aactacgtcg atttaatcaa attaatcatt atcgagataa tttgatggct
    10081 aaattaaatc attttgataa attacaactt aaagagcaac gctttttaac gatttatgat
    10141 tttatattaa atattattgc tatgctttcg atttttggta gtttagttct aggattaatt
    10201 caaattaatg caggccaact aaatattatt tatatgacga gtatagtttt aatggtctta
    10261 actttatttg aacaagctgt accaatgaca aatgtcgcgt attataaagc ggatactgac
    10321 caagcattgc atgatattaa tgaagtgata tctgtacctt ctactaatgg aaaaaaccgt
    10381 cttaatgata agtatgatgc aacgaacatt tatgaagtta aggacgctag ttttaagtat
    10441 tggaaccagc aaacgtatgt gttgtcggat attaatttta atgttaatag aggcgaaaag
    10501 attgcgattg tggggccttc tggttcagga aaaagtacat tactacaaat tatggctggg
    10561 ttatatcaat tagatagtgg ctctattcgt ttcgaaaata tggatatgtt tgaaatagat
    10621 gacaaagata agtttgaatc gttaaatgtc ttgctacaat ctcaacaatt atttgatggt
    10681 acaatacgtc aaaatttatt taccgatgaa aaagatgaag cggtgcaagc aatatttaag
    10741 caattagatt tagaacattt ggcactagaa cgtcaaattg acttagatgg tcatacatta
    10801 tctggcggag aaattcagcg tttagcgatt gcaaggatgt tattaaaaga tactgcatca
    10861 acatggattt tagatgaacc aacaactgca ttagataaac aaaatagttt aaaagttatg
    10921 gatttaattg aagcacatgc agaaacatta attgttgcta cacacgattt aactttattg
    10981 tcacgttttg agaccatcat tgtgatgata aatggtaaaa tagttgaaaa gggaaactat
    11041 caacaattac tcgctaatca aggtgcttta tggaatatga ttcaatataa tgcataaaaa
    11101 aactgcttga taggctaaga aacctttcaa gcagtttttt aactaacaag cttacgcgtg
    11161 ctaattgttt ttctattctt aataaattct aaacattact ttaattgtca tgacaaaagt
    11221 taattatttt tcctttgttt catcaaatgc atgaatgact ttacctaata agcgattaag
    11281 ttctttaact tcatcttgag ataaagaaga agctgaagcg actttgtcag atgcattact
    11341 taattctggt ctaatagttt cacttttgtc agtcaagtga ataaatactt cacgttgatc
    11401 gacttcggaa cgttcacgct taattaagtc tacttgttcc attcgtttta ataatggtga
    11461 tactgtacca gtatcgagtg ctaattcagt tacgactttc ttgacgttta caggagattc
    11521 atcccataaa attgttaaga caagaaattg tgggtatgtt agattgtact tcttaaaaac
    11581 tttgttagag tagtagcgat taacttgtct ttgagcattg tacaaactaa agcatagctg
    11641 ttcttttaaa ttatgttgat cagacattaa agttctcctc cagacatact atccgttttt
    11701 ttctcttttc ggattggtaa tcattaaaaa gttgattgtt tattaattca caactttctt
    11761 tgattcaatg ccatgctaaa attaaagtat gtttaaagtt tagaagatat ttttgattaa
    11821 atca
//
LOCUS       DAMZKC010000081        11706 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.081, whole genome shotgun sequence.
ACCESSION   DAMZKC010000081 DAMZKC010000000
VERSION     DAMZKC010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11706
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.081"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            305..3010
                     /gene="acnA"
                     /locus_tag="POB55_001585"
     CDS             305..3010
                     /gene="acnA"
                     /locus_tag="POB55_001585"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HDI6500549.1"
                     /translation="MAANFKEQSKKHFDLNGQSYTYYDLKAVEEQGITKVSNLPYSIR
                     VLLESLLRQEDDFVITDDHIKALSQFGKDGNEGEVPFKPSRVILQDFTGVPAVVDLAS
                     LRKAMDNVGGDITKINPEVPVDLVIDHSVQVDSYANPEALERNMKLEFERNYERYQFL
                     NWATKAFDNYNAVPPATGIVHQVNLEYLASVVHVRDVDGEKTAFPDTLVGTDSHTTMI
                     NGIGVLGWGVGGIEAEAGMLGQPSYFPIPEVIGVRLVNSLPQGATATDLALRVTQELR
                     KKGVVGKFVEFFGPGVQHLPLADRATIANMAPEYGATCGFFPVDDESLKYMKLTGRSD
                     EHIALVKEYLKQNHMFFDVEKEDPNYTDVIELDLSTVEASLSGPKRPQDLIFLSDMKS
                     SFENSVTAPAGNQGHGLDKSEFDKKAEINFKDGSKATMKTGDIAIAAITSCTNTSNPY
                     VMLGAGLVAKKAVEKGLKVPEYVKTSLAPGSKVVTGYLRDAGLQPYLDDLGFNLVGYG
                     CTTCIGNSGPLLPEIEKAIADEDLLVTSVLSGNRNFEGRIHPLVKANYLASPQLVVAY
                     ALAGTVDIDLQNEPIGKGNDGEDVYLKDIWPSIKEVSDTVDSVVTPELFIEEYNNVYN
                     NNELWNEIDVTDQPLYDFDPNSTYIQNPSFFQGLSKEPGTIVPLNGLRVMGKFGDSVT
                     TDHISPAGAIGKDTPAGKYLQDHQVPIREFNSYGSRRGNHEVMVRGTFANIRIKNQLA
                     PGTEGGFTTYWPTNEVMPIFDAAMKYKEDGTGLVVLAGNDYGMGSSRDWAAKGTNLLG
                     VKTVIAQSYERIHRSNLVMMGVLPLEFKKGESADSLGLDGTEEISVNIDENVQPHDYV
                     KVTAKKQDGDLVEFDAMVRFDSLVEMDYYRHGGILQMVLRNKLAQ"
     gene            3190..3657
                     /locus_tag="POB55_001586"
     CDS             3190..3657
                     /locus_tag="POB55_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HDI6500550.1"
                     /translation="MIYSITEIEARYAETDKMGVIYHGNYATWFEVARLDYISKLGFS
                     YADMEKQGIISPVTDLNVNYKKSIFYPEKVKVKTWVEKYSRLRSVYKYEIFNEKGELA
                     TTGSTELICIKEDTFKPIRLDRYFPDWHEAYSKVQTLNNEGKTVEIMDDIDSL"
     gene            complement(3864..4160)
                     /locus_tag="POB55_001587"
     CDS             complement(3864..4160)
                     /locus_tag="POB55_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HDI6500551.1"
                     /translation="MQIELTDAAVTWFKNELELPENNKVLVFFVRYGGEFQLKQGFSP
                     AFTVEPKEDVDIGYEQQYDDLNVVVAEKDLWYFEDDHIIVNVVDHEDEISYSTK"
     gene            complement(4535..5143)
                     /gene="plsY"
                     /locus_tag="POB55_001588"
     CDS             complement(4535..5143)
                     /gene="plsY"
                     /locus_tag="POB55_001588"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HDI6500552.1"
                     /translation="MMIIVMLLLSYLIGAFPSGFVIGKLFFKKDIRQFGSGNTGATNS
                     FRVLGRPAGFLVTFLDIFKGFITVFFPLWLPVHADGPISTFFTNGLIVGLFAILGHVY
                     PVYLKFQGGKAVATSAGVVLGVNPILLLILAIIFFIVLKIFKYVSLASIVAAICCVIG
                     SLIIQDYILLVVSFLVSIILIIRHRSNIARIFRGEEPKIKWM"
     gene            5349..7340
                     /gene="parE"
                     /locus_tag="POB55_001589"
     CDS             5349..7340
                     /gene="parE"
                     /locus_tag="POB55_001589"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HDI6500553.1"
                     /translation="MNKQNNYSDDSIQVLEGLEAVRKRPGMYIGSTDKRGLHHLVYEI
                     VDNSVDEVLNGYGNEIDVTINKDGSISIEDNGRGMPTGIHKSGKPTVEVIFTVLHAGG
                     KFGQGGYKTSGGLHGVGASVVNALSEWLEVEIHRDGNIYHQSFKNGGSPSSGLVKKGK
                     TKKTGTKVTFKPDDTIFKASTSFNFDVLSERLQESAFLLKNLKITLNDLRSGKERQEH
                     YHYEEGIKEFVSYVNEGKEVLHDVATFSGEANGIEVDVAFQYNDQYSESILSFVNNVR
                     TKDGGTHEVGFKTAMTRVFNDYARRINELKTKDKNLDGNDIREGLTAVVSVRIPEELL
                     QFEGQTKSKLGTSEARSAVDSVVADKLPFYLEEKGQLSKSLVKKAIKAQQAREAARKA
                     REDARSGKKNKRKDTLLSGKLTPAQSKNTEKNELYLVEGDSAGGSAKLGRDRKFQAIL
                     PLRGKVINTEKARLEDIFKNEEINTIIHTIGAGVGTDFKIEDSNYNRVIIMTDADTDG
                     AHIQVLLLTFFFKYMKPLVQADRVFIALPPLYKLEKGKGKTKRVEYAWTDEELNKLQK
                     ELGKGFTLQRYKGLGEMNPEQLWETTMNPETRTLIRVQVEDEVRSSKRVTTLMGDKVQ
                     PRREWIEKHVEFGMQEDQSILDNSEVQVLENDQFDEEEI"
     gene            7340..9742
                     /gene="parC"
                     /locus_tag="POB55_001590"
     CDS             7340..9742
                     /gene="parC"
                     /locus_tag="POB55_001590"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HDI6500554.1"
                     /translation="MSEIIQDLSLEDVLGDRFGRYSKYIIQERALPDVRDGLKPVQRR
                     ILYAMYSSGNTHDKNFRKSAKTVGDVIGQYHPHGDSSVYEAMVRLSQDWKLRHVLIEM
                     HGNNGSIDNDPPAAMRYTEAKLSLLAEELLRDINKETVSFIPNYDDTTLEPMVLPSRF
                     PNLLVNGSTGISAGYATDIPPHNLAEVIQATLKYIDNPDITVNQLMKYIKGPDFPTGG
                     IIQGIDGIKKAYESGKGRIIVRSKVEEETLRNGRKQLIITEIPYEVNKSSLVKRIDEL
                     RADKKVDGIVEVRDETDRTGLRIAIELKKDVNSESIKNYLYKNSDLQISYNFNMVAIS
                     DGRPKLMGIRQIIDSYLNHQIEVVANRTKFELDNAEKRMHIVEGLIKALSILDKVIEL
                     IRSSKNKRDAKENLIEVYEFTEEQAEAIVMLQLYRLTNTDIVALEGEHKELEALIKQL
                     RHILDNHDALLNVIKEELNEIKKKFKSERLSLIEAEIEEIKIDKEVMVPSEEVILSMT
                     RHGYIKRTSIRSFNASGVEDIGLKDGDSLLKHQEVNTQDTVLVFTNKGRYLFIPVHKL
                     ADIRWKELGQHVSQIVPIEEDEVVINVFNEKDFNTDAFYVFATQNGMIKKSTVPLFKT
                     TRFNKPLIATKVKENDDLISVMRFEKDQLITVITNKGMSLTYNTSELSDTGLRAAGVK
                     SINLKAEDFVVVTEGVSENDTILMATQRGSLKRISFKILQVAKRAQRGITLLKELKKN
                     PHRIVAAHVVTGEHSQYTLYSKSNEEHGLINDIHKSEQYTNGSFIVDTDDFGEVIDMY
                     IS"
     gene            9992..11452
                     /locus_tag="POB55_001591"
     CDS             9992..11452
                     /locus_tag="POB55_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HDI6500555.1"
                     /translation="MKDFDSLIPGWFKEFVHVGTDLIWSQYLIGLLLTAGFFFTISSK
                     FVQLRMLPEMFRALVERPETLEDGKKGISPFQAFAISAGSRVGTGNIAGVATAIVLGG
                     PGAVFWMWVIAFIGAASAFIEATLAQVYKVHDKDGGFRGGPAYYITKGLNQKWLGIVF
                     AILITITFAFVFNTVQSNTIAESLNTQYNISPVITGIILAIVTAIIIFGGVRSIATLS
                     SLIVPIMAIIYIGMVLVILLFNLDQIVPMIGTIIKSAFGIEQVTGGAVGAAVLQGIKR
                     GLFSNEAGMGSAPNAAATAAVPHPVKQGLIQSLGVFFDTMLVCTATAIMILLYSGLKF
                     GDNAPQGVAVTQSALNEHLGSAGGIFLTIAVTLFAFSSVVGNYYYGQSNIEFLSTNRV
                     ILFIFRCLVVVLVFVGAVVKTETVWNTADLFMGLMAIVNIISIIGLSNVAFALMKDYQ
                     KQKKEGKNPVFKPENLEINLFGISAWGANKYKNSDK"
BASE COUNT         4096 a         1686 c         2226 g         3698 t
ORIGIN      
        1 cccatcaaaa gaatgacatt gaaaaaaata ttaaagtttg gtgccataat tttctttctg
       61 ctcaatgaaa taatacgttt aaaaattgaa ttgaatcatt cgcgaaattg ataattattc
      121 tcaattaata aaaaatactt attacacctt acctgtatga taagttttgc ttatatactc
      181 tgattaaaaa gtcaaaacct aaattaagcg ttttctaagc atgatgagtt gtttatcaat
      241 aaatataata atacaattta atcaaggcat ataaatataa aaatgtatca agggggatca
      301 ttaaatggct gcaaatttta aagagcaatc aaaaaaacat tttgacttga atggccaaag
      361 ttatacttac tatgatttaa aagctgtaga agagcaaggt attactaaag tttccaattt
      421 accttattca attcgtgttt tgttagaatc tttacttcgt caagaagatg attttgtaat
      481 tacagacgat catattaaag ctttaagtca gtttggaaaa gatggaaatg aaggcgaggt
      541 accatttaaa ccttctcgtg ttattttaca agatttcaca ggtgtaccag ccgtagttga
      601 tttagcttct ttacgtaaag caatggataa cgttggggga gatattacta aaattaatcc
      661 agaagtaccg gtggatttag ttattgacca ctcagttcaa gtggatagct atgcaaatcc
      721 agaagctctt gaacgtaata tgaaattaga atttgaacgt aactatgaac gttatcagtt
      781 tttaaattgg gcaacgaaag catttgataa ttacaatgca gttcctcctg caactggaat
      841 agttcaccaa gttaacttag aatatttagc aagtgttgta catgttcgtg atgtagatgg
      901 tgaaaaaact gcatttccag atacattagt tggtactgat tcacatacaa caatgataaa
      961 tggtattggc gtactaggat ggggtgttgg tggtattgaa gctgaagctg gaatgcttgg
     1021 acaaccttct tatttcccaa ttccagaggt tattggtgta cgactagtaa attcattacc
     1081 acaaggcgca acagcaactg atttagcgtt aagagtaact caagagctac gtaaaaaagg
     1141 tgttgttggt aaatttgtgg agttctttgg tccaggtgta caacatttac cactagcaga
     1201 ccgtgctaca attgcaaaca tggcaccaga gtatggagca acttgcggat tcttcccagt
     1261 tgatgatgaa tctcttaaat atatgaagtt aactggtaga tcagacgaac atatcgcgct
     1321 agtaaaagaa tatttgaaac aaaaccatat gttctttgat gttgagaaag aagatcctaa
     1381 ttatacagat gttatcgaat tggatttatc aacagttgaa gcatcgcttt caggaccaaa
     1441 acgtcctcaa gatttaattt tcttaagtga tatgaaatca tcatttgaaa attctgtaac
     1501 agctccagca ggcaaccaag gacacggttt agataaaagt gaatttgata agaaagctga
     1561 aattaacttt aaagatggat caaaagctac aatgaaaaca ggtgatattg caatagcagc
     1621 aattacatca tgtacaaata catctaaccc ttatgtaatg ttaggtgcag gtttagttgc
     1681 taaaaaagca gttgaaaaag gcttgaaagt tcctgaatac gttaaaactt ctctagcacc
     1741 aggatcaaaa gttgttaccg gatatttaag agatgctggc ttacaacctt atttagatga
     1801 tttaggcttc aacttggttg gttatggatg tacaacttgt atcggtaatt caggtccttt
     1861 attaccagaa attgaaaaag cgattgctga tgaggaccta ttagtgacat ctgtattatc
     1921 tggtaaccgt aactttgaag gtcgtatcca tcctcttgtt aaagccaatt acctagcttc
     1981 accacagtta gttgttgctt atgcattagc tggaacggtt gatattgatt tacagaatga
     2041 acctattggt aaaggtaatg acggtgaaga tgtatatttg aaagatattt ggccatcaat
     2101 taaagaagtt tcagataccg ttgatagtgt tgtaacacct gaattattta ttgaagaata
     2161 taataacgta tacaataaca acgaattatg gaatgagatt gatgtaactg atcaacctct
     2221 atatgacttt gatcctaatt caacatacat tcaaaatcca tcattcttcc aaggattatc
     2281 taaagaaccg ggtacgattg ttccattaaa tggtttacgt gttatgggta aattcggtga
     2341 ttctgtgaca actgaccaca tctctccagc aggtgcaatt ggtaaagata cgccagctgg
     2401 taaatattta caagatcatc aagtgcctat tcgtgaattt aattcatatg gttcaagacg
     2461 tggtaatcac gaagtaatgg ttcgaggtac gtttgctaat atacgtatta aaaaccaatt
     2521 agcgccaggt actgaaggtg gttttacaac ttattggcca acaaatgaag taatgcctat
     2581 ctttgatgct gcaatgaaat ataaagaaga tggtacaggt ttagttgtat tagctggtaa
     2641 cgattatggt atgggttcat ctcgtgactg ggcagcaaaa ggtacaaact tattaggtgt
     2701 taaaacagtt attgcacaaa gttatgaacg tatccatcgt tcaaatttag ttatgatggg
     2761 tgtattacca ttagagttta aaaaaggtga atcagctgat tctcttggtc tagatggtac
     2821 agaagaaatt tctgttaata ttgatgaaaa tgttcaacca catgactacg tcaaagttac
     2881 tgctaagaag caagatggtg atttggtaga atttgacgct atggttcgtt ttgactcact
     2941 tgttgaaatg gattactatc gtcacggtgg aattttacaa atggttttaa gaaataaatt
     3001 agcgcaataa aaaatagata tcacagtaaa attttaatcg gtatttgagg cactttgata
     3061 gtgctcaaaa atacttctat aaactttatg atataatagg tcggttcaaa acctttgtgt
     3121 tcttggccgg cctcttcttg tattttatgt tagaatgaga tataaagtta aactgaaaga
     3181 aggcttaaaa tgatatatag tattacagaa atagaagcgc gttatgctga aactgataag
     3241 atgggtgtaa tttatcacgg gaattatgca acttggtttg aagttgcgcg gttggattat
     3301 atatcgaagt taggttttag ttatgctgat atggaaaaac aaggaatcat ttcacctgtg
     3361 actgacctca atgtcaatta taaaaagtct attttttatc cagaaaaagt taaagttaaa
     3421 acttgggttg aaaaatattc gagattacgt tcagtgtata aatatgagat ttttaatgaa
     3481 aaaggtgaac ttgcaactac aggttccaca gaattgattt gcattaaaga agatactttt
     3541 aagcctatac ggttggatcg ttatttccca gattggcatg aagcttatag taaagtgcaa
     3601 acgctcaata atgaagggaa aacagtagag ataatggatg atattgattc tttataagtt
     3661 actgtaaatg tttggagagg caatattaac aattaatcta taaaaaatag ccaatacgtt
     3721 aacattaatg taattagcgt gaagataagc taatatatag caaaaacgaa cttgttcata
     3781 caacatcaga tgtgctaaac caaagtacaa cttccgaggt tcaggacaca tcagaatgtg
     3841 taaacaagtt cgttttattt tagttatttt gtggaataag aaatttcatc ttcgtgatca
     3901 actacattta caataatgtg gtcatcttca aagtaccaca aatctttttc cgctacgaca
     3961 acatttaaat cgtcatattg ttgttcatag ccaatatcaa catcttcctt tggttcaact
     4021 gtaaaagcag gactaaatcc ttgcttgagt tggaattcgc caccatatct tacaaaaaac
     4081 acgagcactt tattattttc aggcaactca agttcatttt taaaccaagt tactgctgca
     4141 tcagtaagtt ctatttgcat aacaatgacc tcctatattt attacagaat gggagtgggc
     4201 caacttgtat tatttgtgga atttcttatc gaaattctct gtgttggagc ccaccccaac
     4261 ctgctcatta ttgtaagctg aatttccata agcttctatg ttgggacccc cgaccagaat
     4321 tgaaaaaacc tagttataag cgcattttgt tcggttaact acagctaata taacattgtg
     4381 gagcctatga cattgattta tgtctcggtc tcaaatgttc ttgtaaaaaa ctaagtatag
     4441 ttgaatttta agttaaaaaa attgtttttg caaaaataaa ataatccatc aactgcaaaa
     4501 aaatatgcaa tgatggacta atgtcaaaaa attgttacat ccattttatt ttaggttctt
     4561 cgcctctaaa aatccttgcg atattagagc gatgtctaat tatcaatatg attgaaacta
     4621 agaaactaac gactaataaa atatagtctt gaatgataag cgagccaatc acacagcaaa
     4681 ttgctgcaac gatacttgct aaagaaacat atttaaaaat cttcaataca ataaagaaga
     4741 taattgcaag tattagtaaa agtatcggat tgactcccaa gacgacacct gcactagttg
     4801 caacagcttt gccaccttgg aattttaaat aaacaggata aacgtgtcca agtatagcga
     4861 ataagccaac aattaaacca tttgtaaaaa aagtactaat agggccatct gcgtgaactg
     4921 gtaaccataa agggaagaaa acagttatga accctttgaa aatatctaga aatgttacca
     4981 agaatcctgc aggacgacct aatactctaa agctattagt agcgccagta ttaccactac
     5041 caaattgtct aatatctttt ttgaaaaata attttccaat tacgaatcca cttgggaaag
     5101 cgccgataag ataacttagt agtaacatga cgattatcat cataaatatt acacatcctt
     5161 taatatctta ggactatttt atcatattcg tttaaattac ggctaaaaat tttaaaaacg
     5221 gggattaata tatggaatta agctatgaaa gttaattgat acttgcattt tacgctgatt
     5281 tatataagaa taactattgt atagttttaa aaacgaacgt acgtttgcag gaggcgaaat
     5341 cattggcaat gaataaacaa aataattatt cagatgattc aatacaggtt ttagaggggt
     5401 tagaagcagt tcgtaaaaga cctggtatgt atattggatc aactgataaa cggggattac
     5461 atcatctagt atatgaaatt gtcgataact ccgtcgatga agtattgaat ggttacggta
     5521 acgaaataga tgtaacaatt aataaagatg gtagtatttc tatagaagat aatggacgtg
     5581 gtatgccaac aggtatacat aaatcaggta aaccgacagt cgaagttatc tttactgttt
     5641 tacatgcagg aggtaaattt ggacaaggcg gctataaaac ttcaggtggt cttcacggtg
     5701 ttggtgcttc agttgtaaat gcattgagtg aatggcttga agttgaaatc catcgagatg
     5761 gtaatatata tcatcaaagt tttaaaaacg gtggttcgcc atcttctggt ttagtgaaaa
     5821 aaggtaaaac taagaaaaca ggtaccaaag taacatttaa acctgatgac acaattttta
     5881 aagcatctac atcatttaat tttgatgttt taagcgaacg actacaagag tctgcgttct
     5941 tattgaaaaa tttaaaaata acgcttaatg atttacgcag tggtaaagag cgtcaagagc
     6001 attaccatta tgaagaagga atcaaagagt ttgttagtta tgtcaatgaa ggaaaagaag
     6061 ttttgcatga cgtggctaca ttttcaggtg aagcaaatgg tatagaggta gacgtagctt
     6121 tccaatataa tgatcaatat tcagaaagta ttttaagttt tgtaaataat gtacgtacta
     6181 aagatggtgg tacacatgaa gttggcttta aaacagcaat gacacgtgta tttaatgatt
     6241 atgcacgtcg tattaatgaa cttaaaacaa aagataaaaa cttagatggt aatgatattc
     6301 gtgaaggttt aacagctgtt gtgtctgtac gtattccaga agaattatta caatttgaag
     6361 gacaaacgaa atctaaattg ggtacttctg aagctagaag tgctgttgat tcagttgttg
     6421 cagacaaatt gccattctat ttagaagaaa aaggacaatt gtctaaatca cttgtgaaaa
     6481 aagcgattaa agcacaacaa gcaagggaag ctgcacgtaa agctcgtgaa gatgctcgtt
     6541 caggtaagaa aaacaagcgt aaagacactt tgctatctgg taaattaaca cctgcacaaa
     6601 gtaaaaacac tgaaaaaaat gaattgtatt tagtcgaagg tgattctgcg ggaggttcag
     6661 caaaacttgg acgagaccgc aaattccaag cgatattacc attacgtggt aaggtaatta
     6721 atacagagaa agcacgtcta gaagatattt ttaaaaatga agaaattaat acaattatcc
     6781 acacaatcgg ggcaggcgtt ggtactgact ttaaaattga agatagtaat tataatcgtg
     6841 taattattat gactgatgct gatactgatg gtgcgcatat tcaagtgcta ttgttaacat
     6901 tcttcttcaa atatatgaaa ccgcttgttc aagcagatcg tgtatttatt gctttacctc
     6961 cactttataa attggaaaaa ggtaaaggca aaacaaagcg agttgaatac gcttggacag
     7021 acgaagagct taataaatta caaaaagaac ttggtaaagg cttcacgtta caacgttaca
     7081 aaggtttggg tgagatgaac cctgaacaat tatgggaaac gacgatgaac ccagaaacac
     7141 gaactttaat tcgtgtacaa gttgaagatg aagtgcgttc atctaaacgt gtaacaacat
     7201 taatgggtga caaagtacaa cctagacgtg aatggattga aaagcatgtt gagtttggta
     7261 tgcaagagga ccaaagtatt ttagataatt ctgaagtaca agtgcttgaa aatgatcaat
     7321 ttgatgagga ggaaatctag tgagtgaaat aattcaagat ttatcacttg aagatgtttt
     7381 aggtgatcgc tttggaagat atagtaaata tattattcaa gagcgtgcat tgccagatgt
     7441 tcgtgatggt ttaaaaccag tacaacgtcg tattttatat gcaatgtatt caagtggtaa
     7501 tacacacgat aaaaatttcc gtaaaagtgc gaaaacagtc ggtgatgtta ttggtcaata
     7561 tcatccacat ggagactcct cagtgtacga agcaatggtc cgtttaagtc aagactggaa
     7621 gttacgacat gtcttaatag aaatgcatgg taataatggt agtatcgata atgatccgcc
     7681 agcggcaatg cgttacactg aagctaagtt aagcttacta gctgaagagt tattacgtga
     7741 tattaataaa gagacagttt ctttcattcc aaactatgat gatacgacac tcgaaccaat
     7801 ggtattgcca tcaagatttc ctaacttact agtgaatggt tctacaggta tatctgcagg
     7861 ttacgcgaca gatataccac cacataattt agctgaagtg attcaagcaa cacttaaata
     7921 tattgataat ccggatatta cagtcaatca attaatgaaa tatattaaag gtcctgattt
     7981 tccaactggt ggtattattc aaggtattga tggtattaaa aaagcttatg aatcaggtaa
     8041 aggtagaatt atagttcgtt ctaaagttga agaagaaact ttacgcaatg gacgtaaaca
     8101 gttaattatt actgaaattc catatgaagt gaacaaaagt agcttagtaa aacgtatcga
     8161 tgaattacgt gctgacaaaa aagtcgatgg tatcgttgaa gtacgtgatg aaactgatag
     8221 aactggttta cgaatagcaa ttgaattgaa aaaagatgtg aacagtgaat caatcaaaaa
     8281 ttatctttat aaaaactctg atttacagat ttcatataat ttcaacatgg tcgctattag
     8341 tgatggtcgt ccaaaattga tgggtattcg tcaaattata gatagttatt tgaatcacca
     8401 aattgaggtt gttgcaaata gaacgaagtt tgaattagat aatgcagaaa aacgtatgca
     8461 tatcgttgaa ggtttgatta aagcgttgtc aattttagat aaagtaatcg aattgattcg
     8521 tagctctaaa aacaagcgtg acgctaaaga aaaccttatc gaagtatacg agttcacaga
     8581 agaacaggct gaagcaattg taatgttaca gttatatcgt ttaacaaaca ctgatatagt
     8641 tgcgcttgaa ggtgaacata aagaacttga agcattaatc aaacaattac gtcatattct
     8701 tgataaccat gatgcattat tgaatgtcat aaaagaagaa ttgaatgaaa ttaaaaagaa
     8761 attcaaatct gaacgactgt ctttaattga agcagaaatt gaagaaatta aaattgacaa
     8821 agaagttatg gtgcctagtg aagaagttat tttaagtatg acacgtcatg gatatattaa
     8881 acgtacttct attcgtagct ttaatgctag cggtgttgaa gatattggtt taaaagatgg
     8941 tgacagttta cttaaacatc aagaagtaaa tacgcaagat accgtactag tatttacaaa
     9001 taaaggtcgt tatctattta taccggttca taaattagca gatattcgtt ggaaagaatt
     9061 gggacaacat gtatcacaaa tagttcctat cgaagaagat gaagtggtta ttaatgtctt
     9121 taatgaaaag gactttaata cagatgcatt ttatgttttt gcgactcaaa atggcatgat
     9181 taagaaaagt acagtgcctc tatttaaaac aacgcgtttt aataaacctt taattgctac
     9241 taaagttaaa gaaaatgatg atttgattag tgttatgcgc tttgaaaaag atcaattaat
     9301 taccgtcatt actaataaag gtatgtcatt aacgtataat acaagtgaac tatcagatac
     9361 cggattaagg gcagctggtg ttaaatcaat aaatcttaaa gctgaagatt tcgttgttgt
     9421 gacagaaggt gtttctgaaa atgatactat attgatggcc acacaacgcg gctcgttaaa
     9481 acgtattagt tttaaaatct tacaagttgc taaaagagca caacgtggaa taactttatt
     9541 aaaagaatta aagaaaaatc cacatcgtat tgtagctgca catgtagtga caggtgaaca
     9601 tagtcaatat acattatatt caaaatcaaa tgaagaacat ggtttaatta atgatattca
     9661 taaatctgaa caatatacaa atggctcatt cattgtagat acagatgatt ttggtgaagt
     9721 aatagacatg tatattagct aaaaactata tgcaatcacg aaattaaatg ataaaataca
     9781 gtaatgttaa attttgacta aattcaaggg atttatatta aatgctgacc aagtacttat
     9841 cgttaaatta gcgatatact ttgaccaaac taatataaat ctcttgtatt gtataagaaa
     9901 tgatgatgta tctctatatt tactgaagtt tcaattcggt ctaattttat agaaggtaca
     9961 tgattcatgt agaggatgcg aggggtttat tttgaaagat ttcgatagtt taattcctgg
    10021 atggtttaaa gaatttgtcc atgttggtac cgatttaata tggtctcaat atttaattgg
    10081 tctattattg acagctggat tcttctttac aattagttct aaattcgtcc aattacgaat
    10141 gttacctgaa atgtttagag ctttagtaga acgtccagaa actttagaag atggtaagaa
    10201 gggtatttcg ccattccaag catttgcgat tagtgctggt tcgagagttg gtactggtaa
    10261 tattgctggt gttgcgactg cgattgtttt aggcggtcca ggtgcagtgt tttggatgtg
    10321 ggttattgca tttataggtg cagcgagtgc atttatagaa gcgactttgg ctcaggttta
    10381 taaagtacat gataaagatg gtggattccg tggtggtcca gcttactata ttactaaagg
    10441 tttaaatcaa aaatggctag gtatcgtatt tgcgatttta attacaatta catttgcatt
    10501 tgtatttaac acagtgcaat ctaatacaat tgcggagtcg ttaaatacgc aatataatat
    10561 tagtccagta atcacaggta ttattttagc aatcgtaaca gctattatta tatttggtgg
    10621 tgtacgtagt attgctacgt tatcttcgtt aattgtaccg attatggcta tcatttacat
    10681 tggtatggtt ttagtaatat tgctatttaa tttagatcaa attgttccta tgataggtac
    10741 gattattaaa agtgcatttg gtatcgaaca agtaactggt ggcgctgtag gtgctgcggt
    10801 tcttcaaggt atcaaacgtg gtttattctc taacgaagct ggtatgggtt ctgcgccgaa
    10861 tgcagcggca actgctgccg taccacaccc tgttaagcaa ggtttaatcc aatcattagg
    10921 tgtgttcttt gatacaatgt tggtttgtac agcaactgca atcatgattt tactatattc
    10981 aggactgaaa tttggtgata acgcacctca aggtgttgca gttactcaat cagcacttaa
    11041 tgagcattta ggttctgctg gaggtatttt cttaacaata gcagttacac tgtttgcatt
    11101 ttcatctgtg gtaggtaatt actattacgg tcaatctaat attgaatttt tatcaacaaa
    11161 ccgtgtaata ttatttatct ttagatgtct tgttgtagta cttgtctttg tcggtgcagt
    11221 tgtaaaaaca gaaacagtat ggaatacggc agacttattt atgggcttaa tggcaattgt
    11281 aaacattatt tccattatag gactgtccaa tgtagctttt gcattgatga aagattatca
    11341 aaagcagaaa aaagaaggca agaaccctgt ctttaaacct gaaaacttag aaattaactt
    11401 atttggaatt agtgcttggg gcgctaacaa atataagaac tctgataaat aaatattttt
    11461 aatgtaacga ttcattatat aaaaatcaaa taaaccctga ggttttcaaa ttaaagcgaa
    11521 aatctcaggg ttttaaattt gtactcaata atcatctgta tcattacact agttaatcta
    11581 agatttgatt atatattaat atagtaaggt tgagatttat ttcctccatt ggtgtgcaaa
    11641 gactatacca ttgataagac atattgttgg atagatgaat ataaatttat tttagctttg
    11701 cacaat
//
LOCUS       DAMZKC010000082        11581 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.083, whole genome shotgun sequence.
ACCESSION   DAMZKC010000082 DAMZKC010000000
VERSION     DAMZKC010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11581
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.083"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            259..1947
                     /gene="ilvD"
                     /locus_tag="POB55_001592"
     CDS             259..1947
                     /gene="ilvD"
                     /locus_tag="POB55_001592"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HDI6500556.1"
                     /translation="MRSDMIKKGDHQAPARSLLHATGALKSPTDMNKPFVAICNSYID
                     IVPGHVHLRELADIAKEAIREAGAIPFEFNTIGVDDGIAMGHIGMRYSLPSREIIADA
                     AETVINAHWFDGVFYIPNCDKITPGMILAAMRTNVPAIFCSGGPMKAGLSAHGKALTL
                     SSMFEAVGAFKEGSISKEEFLDMEQNACPTCGSCAGMFTANSMNCLMEVLGLALPYNG
                     TALAVSDQRREMIRQAAFKLVENIKNDLKPRDIVTREAIDDAFALDMAMGGSTNTVLH
                     TLAIANEAGIDYDLERINAIAKRTPYLSKIAPSSSYSMHDVHEAGGVPAIINELMKKD
                     GTLHPDRITVTGKTLRENNEGKEIKNFDVIHPLDAPYDAQGGLSILFGNIAPKGAVIK
                     VGGVDPSIKTFTGKAICFNSHDEAVEAIDNRTVRAGHVVVIRYEGPKGGPGMPEMLAP
                     TSSIVGRGLGKDVALITDGRFSGATRGIAVGHISPEAASGGPIALIEDGDEITIDLTN
                     RTLNVNQPEDVLARRRESLTPFKAKVKTGYLARYTALVTSANTGGVMQVPENLI"
     gene            1975..3744
                     /gene="ilvB"
                     /locus_tag="POB55_001593"
     CDS             1975..3744
                     /gene="ilvB"
                     /locus_tag="POB55_001593"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HDI6500557.1"
                     /translation="MSKTQHEVNQNIDPLKMAESLELEQLNEKTLNDMRSGSEVLVEA
                     LLKENVDYLFGYPGGAVLPLYDTFYDGKIKHILARHEQGAVHAAEGYARVSGKTGVVV
                     VTSGPGATNVMTGITDAHCDSLPLVVFTGQVATPGIGKDAFQEADILSMTSPITKQNY
                     QVKRVEDIPKIVHEAFHVANSGHKGPVVIDFPKDMGVLATNVDLCDEINIPGYEVVTE
                     PENKDIDTFISLLKEAKKPVVLAGAGINQSKSNQLLTQFVNKHQIPTVTTLLGLGAVP
                     YEDTLFLGMGGMHGSYASNMALTECDLLINLGSRFDDRLASKPDAFAPNAKIVHVDID
                     PSEINKVIHVDLGIIADCKRFLECLNDKNVETIEHSDWVKHCQNNKQKHPFKLGEEDQ
                     VFCKPQQTIEYIGKITNGEAIVTTDVGQHQMWAAQFYPFKNHGQWVTSGGLGTMGFGI
                     PSSIGAKLANPDKTVVCFVGDGGFQMTNQEMALLPEYGLDVKIVLINNGTLGMVKQWQ
                     DKFFNQRFSHSVFNGQPDFMKMAEAYGVKGFLIDKPEQLEEQLDAAFAYQGPALIEVR
                     ISPTEAVTPMVPSGKSNHEMEGL"
     gene            3744..3998
                     /locus_tag="POB55_001594"
     CDS             3744..3998
                     /locus_tag="POB55_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HDI6500558.1"
                     /translation="MTRILKLQVADQVSTLNRITSAFVRLQYNIDTLHVTHSEQPGIS
                     NMEIQVDIQDDTSLHILIKKLKQQINVLTVECYDLVDNEA"
     gene            4135..5139
                     /gene="ilvC"
                     /locus_tag="POB55_001595"
     CDS             4135..5139
                     /gene="ilvC"
                     /locus_tag="POB55_001595"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HDI6500559.1"
                     /translation="MTTVYYDQDVKTDALQGKKIAVVGYGSQGHAHAQNLKDNGYDVV
                     IGIRPGRSFDKAKEDGFDVFPVAEAVKQADVIMVLLPDEIQGDVYKNEIEPNLEKHNA
                     LAFAHGFNIHFGVIQPPADVDVFLVAPKGPGHLVRRTFVEGSAVPSLFGIQQDASGQA
                     RNIALSYAKGIGATRAGVIETTFKEETETDLFGEQAVLCGGVSKLIQSGFETLVEAGY
                     QPELAYFEVLHEMKLIVDLMYEGGTENVRYSISNTAEFGDYVSGPRVITPDVKENMKA
                     VLTDIQNGNFSNRFIEDNKNGFKEFYKLREEQHGHQIEKVGRELREMMPFIKSKSIEK
                     "
     gene            5169..6698
                     /locus_tag="POB55_001596"
     CDS             5169..6698
                     /locus_tag="POB55_001596"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HDI6500560.1"
                     /translation="MSSHIQIFDTTLRDGEQTPGVNFTFDERLRIALQLEKWGVDVIE
                     AGFPASSTGSFKSVQAIAQTLTTTAVCGLARCKKSDIDAVYEATKDAAKPVVHVFIAT
                     SPIHLEHKLKMSQEDVLASIKEHVTYAKQLFDVVQFSPEDATRTELPFLVKCVQTAVD
                     AGATVINIPDTVGYSYHDEYAHIFKTLTESVTSSNEIIYSAHCHDDLGMAVSNSLAAI
                     EGGARRIEGTVNGIGERAGNAALEEVALALYVRNDHYGAQTALNLEETKKTSDLISRY
                     AGIRVPRNKAIVGQNAFSHESGIHQDGVLKHRETYEIMTPQLVGVSTTELPLGKLSGK
                     HAFSEKLKALGYGIDKEAQIDLFKQFKAIADKKKSVSDRDIHAIIQGSEHEHQALYKL
                     ETLQLQYVSSGLQSAVVVVKDKEGHIYQDSSIGTGSIVAIYNAVDRIFQKETELIDYR
                     INSVTEGTDAQAEVHVNLLIEGKTVNGFGIDHDILQASCKAYVEAHAKFAAENVEKVG
                     N"
     gene            6701..7747
                     /gene="leuB"
                     /locus_tag="POB55_001597"
     CDS             6701..7747
                     /gene="leuB"
                     /locus_tag="POB55_001597"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HDI6500561.1"
                     /translation="MTYNIVALPGDGIGPEILNGSLSLLEIISNKYNFNYQIEHHEFG
                     GASIDTFGEPLTEKTLNACKRADAILLGAIGGPKWTDPNNRPEQGLLKLRKSLNLFAN
                     IRPTTVVKGASSLSPLKEERVEGTDLVIVRELTSGIYFGEPRHFNNHEALDSLTYTRE
                     EIERIVHVAFKLAASRRGKLTSVDKENVLASSKLWRKVVNEVSQLYPEVTVNHLLVDA
                     CSMHLITNPKQFDVIVCENLFGDILSDEASVIPGSLGLSPSASFSNDGPRLYEPIHGS
                     APDIAGKNVANPFGMILSLAMCLRESLNQPDAADELEQHIYNMIEHGQTTADLGGKLN
                     TTDIFEILSQKLNH"
     gene            7761..9131
                     /gene="leuC"
                     /locus_tag="POB55_001598"
     CDS             7761..9131
                     /gene="leuC"
                     /locus_tag="POB55_001598"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HDI6500562.1"
                     /translation="MGQTLFDKVWNRHVLYGKLGEPQLLYIDLHLIHEVTSPQAFEGL
                     RLQNRKLRRPDLTFATLDHNVPTIDIFNIKDEIANKQITTLQKNAIDFGVHIFDMGSD
                     EQGIVHMVGPETGLTQPGKTIVCGDSHTATHGAFGAIAFGIGTSEVEHVFATQTLWQT
                     KPKNLKIDINGTLPTGVYAKDIILHLIKTYGVDFGTGYALEFTGETIKNLSMDGRMTI
                     CNMAIEGGAKYGIIQPDDITFEYVKGRPFADNFVKSVDKWRELYSDDDAIFDRVIELD
                     VSTLEPQVTWGTNPEMGVNFSEPFPEISDINDQRAYDYMGLEPGQKAEDIDLGYVFLG
                     SCTNARLSDLIEASHIVKGNKVHPNITAIVVPGSRTVKKEAEKLGLDTIFKNAGFEWR
                     EPGCSMCLGMNPDQVPEGVHCASTSNRNFEGRQGKGARTHLVSPAMAAAAAIHGKFVD
                     VRKVVV"
     gene            9132..9704
                     /gene="leuD"
                     /locus_tag="POB55_001599"
     CDS             9132..9704
                     /gene="leuD"
                     /locus_tag="POB55_001599"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HDI6500563.1"
                     /translation="MAAIKPITTYKGKIVPLFNDNIDTDQIIPKVHLKRISKSGFGPF
                     AFDEWRYLPDGSDNPDFNPNKPQYKGASILITGDNFGCGSSREHAAWALKDYGFHIII
                     AGSFSDIFYMNCTKNAMLPIVLEKNAREHLAKYVEIEVDLPNQTVSSPDKRFHFEIDE
                     TWKNKLVNGLDDIAITLQYESLIEKYEKSL"
     gene            9719..10987
                     /gene="ilvA"
                     /locus_tag="POB55_001600"
     CDS             9719..10987
                     /gene="ilvA"
                     /locus_tag="POB55_001600"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HDI6500564.1"
                     /translation="MTVKTTVSTKDIDEAFLRLKDIVKETPLQLDHYLSQKYDCKVYL
                     KREDLQWVRSFKLRGAYNAISVLSDEAKSKGITCASAGNHAQGVAYTAKKLNLNAVIF
                     MPVTTPLQKVNQVKFFGNSNVEVVLTGDTFDHCLAEALTYTSEHQMNFIDPFNNVHTI
                     SGQGTLAKEMLEQSKTDNVNFDYLFAAIGGGGLISGISTYFKTYSPTTKIIGVEPSGA
                     SSMYESVVVNNQVVTLPNIDKFVDGASVARVGDITFEIAKENVDDYVQVDEGAVCSTI
                     LDMYSKQAIVAEPAGALSVSALENYKDHIKGKTVVCVISGGNNDINRMKEIEERSLLY
                     EEMKHYFILNFPQRPGALREFVNDVLGPQDDITKFEYLKKSSQNTGTVIIGIQLKDHD
                     DLIQLKQRVNHFDPSNIYINENKMLYSLLI"
     gene            complement(11454..11568)
                     /gene="rrf"
                     /locus_tag="POB55_001601"
     rRNA            complement(11454..11568)
                     /gene="rrf"
                     /locus_tag="POB55_001601"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         3863 a         1943 c         2236 g         3539 t
ORIGIN      
        1 acggaaggag tataataaaa tgcttaatca atatactgaa catcaaccga caacttcaaa
       61 tattattatt ttattatact ctttaggact cgaacgttag taaatattta ctaaacgctt
      121 taagtcctat ttctgtttga atgggacttg taaacgtccc aatattattg ggacgttttt
      181 ttatgtttta tctttcaatt acttattttt attactataa aacatgatta atcattaaaa
      241 tttacggggg aatttactat gcgaagcgac atgatcaaaa aaggagatca ccaagcacca
      301 gcaagaagtc ttttacatgc cacgggcgcg ctaaaaagtc caactgatat gaacaaacca
      361 tttgtagcta tttgtaactc ttatattgat attgttcctg gacatgttca cttgagagag
      421 cttgcagata tagctaaaga agcaattaga gaagccggtg ccattccatt tgaattcaat
      481 acaattggtg ttgatgatgg aatagctatg ggacatatcg gaatgcgata ttctctacca
      541 tcacgtgaaa ttattgcaga tgcagctgaa actgtaatta acgctcattg gtttgacggc
      601 gtattttaca ttcctaattg tgacaagatt acacccggta tgattttagc agccatgagg
      661 acaaacgtac cagctatctt ttgctctggt ggaccaatga aagctggctt atctgcacat
      721 ggaaaagcat taacactttc atcaatgttt gaagcagtcg gcgcatttaa agaaggatcg
      781 atttctaaag aagaattttt agatatggaa caaaatgcct gccctacttg tggttcatgt
      841 gctgggatgt ttactgcaaa ttcaatgaac tgtttgatgg aagttttagg tctagcatta
      901 ccatacaacg gtactgcact tgcagtcagt gatcagcgac gagaaatgat tcgccaagca
      961 gcttttaaat tagttgaaaa tattaaaaat gatttaaaac cacgtgatat cgttactcgc
     1021 gaagcaattg atgatgcatt tgcacttgat atggctatgg gtggttcaac aaacacggta
     1081 ctgcatacgt tagccattgc caatgaagct ggtattgatt atgacttaga gcgcattaat
     1141 gctattgcca aacgcacgcc atatttatca aaaatagcac ctagttcatc gtattcaatg
     1201 catgatgtgc atgaagctgg tggcgtccca gcaattatta atgaattgat gaagaaagat
     1261 ggcacgttac acccagatag aatcacagtt actggcaaaa cgttacgtga aaataacgaa
     1321 ggcaaagaaa ttaagaactt tgatgtcatt caccctcttg atgcaccata tgatgcacaa
     1381 ggcggtttat ctatcttatt tggtaatatc gcccctaaag gcgcagttat taaagttggc
     1441 ggcgttgatc catctatcaa aacatttact gggaaagcaa tttgtttcaa ttcgcatgat
     1501 gaagctgttg aagcaataga caatcgtacc gttcgtgcag gccacgtcgt tgtcattaga
     1561 tatgaaggac ctaaaggtgg accaggtatg cctgaaatgt tagcacctac ttcctctatt
     1621 gttggtcgcg gcttaggtaa agatgttgca ttaattactg atgggcgttt ttccggtgcc
     1681 acaagaggta ttgcagttgg tcatatttcc cctgaagctg catctggtgg accaattgcc
     1741 ttaattgaag atggagatga gattactatt gatttaacaa atcgtacatt aaacgtaaac
     1801 cagcctgaag atgttctagc gcgtcgccga gaatctttaa caccgtttaa agcgaaagta
     1861 aaaacaggtt atctagctcg ttatactgcc ctagtaacta gcgcaaatac aggtggcgtc
     1921 atgcaagtcc ctgagaattt aatttaattt atttttatat tggagatggt taaaatgtct
     1981 aaaactcaac atgaagtaaa ccaaaatatt gaccctttaa aaatggctga atcacttgaa
     2041 cttgaacaac taaatgaaaa aactttaaat gacatgcgtt caggatcaga agtgctagta
     2101 gaagctctac ttaaagaaaa tgtggattat ttattcggtt atcctggtgg tgccgtacta
     2161 cctttatacg acacatttta tgatggtaaa atcaaacata ttttagcaag acacgaacaa
     2221 ggtgctgttc atgctgcaga aggttatgca cgtgtatctg gtaaaactgg cgtcgttgta
     2281 gttacaagcg gtccaggtgc aactaatgta atgacaggta ttacggatgc acattgcgac
     2341 tctttacctc tagttgtatt cactggacaa gttgctacac caggcattgg taaagatgca
     2401 ttccaagaag cggatattct atctatgact tcaccaatta caaaacaaaa ttatcaagtg
     2461 aaacgtgttg aagatatccc taaaatcgta cacgaagctt tccatgtagc taattctgga
     2521 cacaaaggtc ctgtagtgat tgattttcca aaagatatgg gtgttttagc tacaaatgtg
     2581 gatttatgcg acgaaatcaa tattccaggt tatgaagttg ttacagaacc agaaaataaa
     2641 gacattgaca ctttcatctc acttttaaaa gaagcgaaaa agcctgtcgt attagccggc
     2701 gcaggtatta atcaatcaaa atcaaatcaa ttattaacac agtttgttaa taaacatcaa
     2761 atcccaacag ttactacttt actaggtcta ggtgctgtac cttatgagga tacactattt
     2821 ttaggtatgg gaggtatgca tggttcttat gctagtaaca tggcattaac tgagtgtgat
     2881 ttactcatta atttaggtag ccgcttcgat gatagattag caagcaaacc tgatgccttt
     2941 gcacctaacg ccaaaattgt acatgtagat attgatcctt cagaaatcaa taaagttatt
     3001 catgtagatt taggtattat tgcagactgt aaaagatttt tagaatgttt aaatgataaa
     3061 aatgttgaga ctatagaaca cagtgactgg gttaaacatt gtcaaaataa taagcagaaa
     3121 cacccattta aacttggtga agaagatcaa gtattttgta agccacaaca aacaatcgaa
     3181 tatatcggca aaattacaaa tggtgaagca attgttacta cagacgtggg acaacatcaa
     3241 atgtgggcag ctcaatttta tccatttaaa aatcacggac aatgggttac aagcggtggt
     3301 ttaggaacaa tgggattcgg tattccttcg tcaattggtg ccaaattagc taatcctgat
     3361 aaaacagtcg tatgtttcgt cggtgacggt ggtttccaaa tgacaaacca agaaatggca
     3421 cttttacccg aatatggttt agatgtcaaa atcgtactaa tcaataatgg aacattaggt
     3481 atggttaaac aatggcaaga taagttcttt aatcaacgct tctcacactc agtatttaat
     3541 ggtcaacctg attttatgaa aatggcagaa gcatatggcg tcaaaggttt cttaatcgat
     3601 aagccagaac aactggaaga acaattagat gcagcgtttg cttatcaagg accagcttta
     3661 attgaggttc gtatttcccc tactgaagct gtaaccccaa tggttccgag tggcaaatca
     3721 aatcatgaaa tggagggctt ataatgacaa gaattcttaa attacaagtt gcggatcaag
     3781 tcagcacgct aaatcgaatt acaagtgctt ttgttcgcct acaatataat atcgatacat
     3841 tacatgttac acattctgaa caacctggga tttctaacat ggaaattcaa gtcgatattc
     3901 aagatgatac atcacttcat atattaatta aaaaattaaa acaacaaatt aatgttttaa
     3961 cggttgaatg ctacgacctt gttgataacg aagcttaatt ttaagacaaa ggcaatgatg
     4021 cgctaattag ttatagataa atcataggct gctagttaac atctgccact attacaaagt
     4081 tatatttcag aattttcgaa aaacaaaata tttaattatt tggaggaatt tattatgaca
     4141 acagtttatt atgatcaaga tgtaaaaacg gacgctttac aaggcaaaaa aattgcagta
     4201 gtaggttatg gatcacaagg tcacgcgcat gcacaaaact taaaagacaa tggatatgat
     4261 gtagtcatcg gcattcgccc aggtcgttct tttgacaaag ctaaagaaga tggatttgat
     4321 gtgttccctg ttgcagaagc agttaagcaa gctgatgtaa ttatggtgct attacctgat
     4381 gaaattcaag gtgatgtata caaaaacgaa attgaaccaa atttagaaaa acataatgcg
     4441 cttgcatttg ctcatggctt taacattcat tttggtgtta ttcaaccacc agctgatgtt
     4501 gatgtatttt tagtagctcc taaaggaccg ggtcatttag ttagacgtac atttgttgaa
     4561 ggttctgctg taccatcact atttggtatt caacaagacg cttcaggtca agcacgtaat
     4621 attgctttaa gttatgcaaa aggtattggt gcaactcgtg caggtgttat tgaaacaaca
     4681 tttaaagaag aaactgagac agatttattt ggtgaacaag cagtactttg cggtggtgta
     4741 tcgaaattaa ttcaaagtgg ctttgaaaca ttagtagaag cgggttatca accagaatta
     4801 gcttattttg aagtattaca tgaaatgaaa ttaatcgttg atttgatgta tgaaggcggt
     4861 acggaaaatg tacgttactc aatttcaaat actgctgaat ttggtgacta tgtttcagga
     4921 ccacgtgtta tcacaccaga tgttaaagaa aatatgaaag ctgtattaac tgatatccaa
     4981 aatggtaact tcagtaatcg ctttatcgaa gacaataaaa atggattcaa agaattttat
     5041 aaattacgcg aagaacaaca tggtcatcaa attgaaaaag ttggtcgtga attacgcgaa
     5101 atgatgcctt ttattaaatc taaaagcatt gaaaaataag atagacctac aatgaggagt
     5161 tgttaaatat gagtagtcat attcaaattt ttgatacgac actaagagac ggtgaacaaa
     5221 caccaggagt gaattttact tttgatgaac gcttgcgtat tgcattgcaa ttagaaaaat
     5281 ggggtgtaga tgttattgaa gctggatttc ctgcttcaag tacaggtagc tttaaatctg
     5341 ttcaagcaat tgcacaaaca ttaacaacaa cggctgtatg tggtttagct agatgtaaaa
     5401 aatctgacat cgatgctgta tatgaagcaa caaaagatgc agcgaagccg gtcgtgcatg
     5461 tttttatagc aacatcacct attcatcttg aacataaact taaaatgtct caagaagacg
     5521 ttttagcatc tattaaagaa catgtcacat acgcgaaaca attatttgac gttgttcaat
     5581 tttcacctga agatgcaacg cgtactgaat taccattctt agtgaaatgt gtacaaactg
     5641 ccgttgacgc tggagctaca gttattaata ttcctgatac agtcggctac agttaccatg
     5701 atgaatatgc acatattttc aaaaccttaa cagaatctgt aacatcttca aatgaaatta
     5761 tttatagtgc tcattgccat gacgatttag gaatggctgt ttcaaatagt ttagctgcaa
     5821 ttgaaggcgg tgcgagacga attgaaggca ctgtaaatgg tattggtgaa cgagcaggta
     5881 atgcagcact tgaagaagtc gcgcttgcac tatacgttcg aaatgatcat tatggtgctc
     5941 aaactgctct taatctcgaa gaaactaaaa aaacatcgga tttaatttca agatatgcag
     6001 gtattcgagt gcctagaaat aaagcaattg ttggccaaaa tgcatttagt catgaatcag
     6061 gtattcacca agatggcgta ttaaaacatc gtgaaacata tgaaattatg acacctcaac
     6121 ttgttggtgt aagcacgact gaacttccat taggaaaatt atctggtaaa cacgccttct
     6181 cagagaagtt aaaagcatta ggttatggca ttgataaaga agcgcaaata gatttattta
     6241 aacaattcaa ggccattgcg gacaaaaaga aatctgtttc agatagagat attcatgcga
     6301 ttattcaagg ttctgagcat gagcatcaag cactttataa attggaaaca ctgcaactac
     6361 aatatgtctc tagcggcctt caaagtgctg ttgttgttgt taaagataaa gagggtcata
     6421 tttaccagga ttcaagtatt ggtactggct caatcgtagc aatttacaat gcagttgatc
     6481 gtattttcca gaaagaaaca gaattaattg attatcgtat taattctgtc actgaaggta
     6541 ctgatgccca agcagaagta catgtaaatt tattgattga aggtaagact gtcaatggct
     6601 ttggtattga tcatgatatt ttacaagcct cttgtaaagc atacgtagaa gcacatgcta
     6661 aatttgcagc tgaaaatgtt gagaaggtag gtaattaatt atgacttata acattgttgc
     6721 cctacctggt gatggaatcg gtccagaaat tttgaacgga tctctatcat tgcttgaaat
     6781 tataagtaat aaatataact ttaattatca aatagagcac cacgaatttg gtggtgcctc
     6841 tattgataca ttcggcgaac ctttaactga gaaaacctta aatgcgtgta aaagagcaga
     6901 tgctatttta ctgggtgcaa tcggtggacc taaatggaca gatcctaaca atcgaccaga
     6961 acaaggatta ttaaaattgc gtaaatcctt aaatttattt gcaaatatac gccccactac
     7021 cgttgtcaaa ggcgctagtt ctttatcacc tttaaaggaa gaacgcgttg aaggcacaga
     7081 tttagttata gtccgtgaat tgacaagtgg tatttatttt ggagaaccta gacattttaa
     7141 taatcacgag gccttagatt ctcttactta tacaagagaa gaaatagaac gcattgttca
     7201 cgtagcattt aaattggccg cttcaagacg aggaaaacta acatcagttg ataaagaaaa
     7261 tgtattagct tctagtaaat tgtggcgcaa agtcgtaaat gaagtaagtc aattatatcc
     7321 agaagtaaca gtaaatcact tacttgttga tgcttgtagt atgcatttaa tcacaaatcc
     7381 aaaacaattt gacgtcatcg tatgtgaaaa cttatttggc gatattttaa gtgatgaagc
     7441 ttcagtgatt cctggttcac ttggtttatc accttctgct agttttagta acgatggtcc
     7501 aagattgtat gagcctattc atggatcagc accagatatt gcaggtaaaa acgttgccaa
     7561 tccatttgga atgattctat ctttagcgat gtgtttacgt gaaagcttaa atcaaccaga
     7621 tgctgcagat gaattagaac aacatattta taacatgatt gaacatgggc aaacgacagc
     7681 agatttaggc ggcaaattga atactactga tattttcgaa attctatctc aaaaattgaa
     7741 tcactaaggg ggagatgtaa atgggtcaaa cattatttga caaggtgtgg aacagacatg
     7801 tgttatacgg gaaattgggc gaaccgcaac tattatacat tgatttacac cttatacatg
     7861 aagttacttc tcctcaagca tttgaaggac ttaggcttca aaacagaaaa ttaagacgcc
     7921 cagatttaac atttgcaaca ctcgatcaca atgttcctac tattgatata ttcaatatta
     7981 aagatgaaat tgcaaacaaa caaatcacaa cattacaaaa aaacgccata gattttgggg
     8041 tgcatatttt tgatatgggt tctgatgaac aaggtattgt tcacatggta ggacctgaga
     8101 caggacttac acagcctgga aagacaatcg tttgtggtga ctctcacaca gcaacacatg
     8161 gagcatttgg tgctattgca tttggaattg gaacaagtga agttgaacat gttttcgcaa
     8221 ctcaaacgct atggcaaaca aaacccaaaa acttaaaaat cgatattaat ggtaccttac
     8281 caacaggcgt ctatgctaag gacattattc tgcatttaat taaaacgtat ggtgttgact
     8341 ttggtacagg ctatgctttg gaatttactg gcgaaacaat taaaaacctt tcaatggacg
     8401 gtcgaatgac tatttgtaac atggctatcg aaggtggtgc caaatacggc ataatccaac
     8461 ctgatgatat aacatttgaa tatgttaaag ggagaccatt tgccgataac ttcgttaaat
     8521 cagttgataa gtggcgtgag ctatattctg atgacgacgc gatatttgat cgtgtaattg
     8581 aacttgatgt ttcaacatta gaaccacaag tgacatgggg aactaatcct gaaatgggtg
     8641 ttaatttcag tgaaccattc cctgaaatca gtgatatcaa cgatcaacgt gcgtatgatt
     8701 atatggggtt agaaccagga caaaaagctg aagatatcga cttagggtat gttttcctcg
     8761 gttcatgtac gaatgctaga ctatcagatt tgattgaagc tagtcatatt gttaaaggaa
     8821 ataaagttca cccaaatatt acagctattg tcgtgccagg ttctcgtaca gtaaaaaaag
     8881 aagctgaaaa attaggtcta gatactatct ttaaaaatgc aggatttgaa tggcgtgaac
     8941 caggatgttc aatgtgttta ggcatgaatc ctgaccaagt acctgagggc gtacattgtg
     9001 catctacaag taatcgaaac tttgaaggac gacaaggtaa aggtgcaaga acacatttag
     9061 tatcccctgc tatggcagca gcagcagcta ttcatggtaa atttgtggac gtaagaaagg
     9121 tggttgttta aatggcagca atcaaaccta ttacaacata taaaggtaaa atagtccctc
     9181 tcttcaacga caatatcgat acagaccaaa tcattcctaa ggtacactta aagcgtattt
     9241 caaaaagtgg tttcggtcca tttgcttttg atgaatggcg gtacttacct gatggttcag
     9301 ataatccaga tttcaatcct aacaaaccac aatataaagg ggcttctatt ttaattacag
     9361 gagataattt tggatgcggt tcaagtcgtg aacatgctgc ttgggctctt aaggactatg
     9421 gttttcatat tattattgca ggaagtttca gtgacatatt ttatatgaat tgtacaaaaa
     9481 atgcaatgtt accaattgtt ttagaaaaaa atgcacgtga acatcttgct aaatatgttg
     9541 aaattgaggt cgatttacca aatcaaactg tgtcatcacc agacaagcgt ttccattttg
     9601 aaattgatga aacttggaaa aataaacttg taaatggctt agatgacatt gcaatcaccc
     9661 tacaatatga atcattaata gaaaaatatg aaaaatcact ttaagggagt tgaatattat
     9721 gacagtcaaa acaacagttt ctacgaaaga tatcgatgaa gcatttttaa gacttaaaga
     9781 tattgtcaaa gaaacacctt tacaattaga ccattactta tctcaaaagt atgattgtaa
     9841 agtttattta aaacgagaag atttacaatg ggtacgttct tttaaattaa gaggtgctta
     9901 caacgctatt tctgttttgt cagatgaagc taaaagtaaa ggtattacat gtgcgagtgc
     9961 aggtaatcat gctcaaggtg ttgcctatac agctaaaaaa cttaatttaa acgctgttat
    10021 ctttatgcca gtcactacac ctttacaaaa ggtaaatcaa gtaaagttct ttggaaatag
    10081 taacgttgaa gttgtactca ctggtgatac atttgatcac tgtttagctg aagctttaac
    10141 ttatacaagt gaacatcaaa tgaactttat agatccattc aataatgttc atacaatttc
    10201 tggacaaggt acgcttgcta aagaaatgct agaacaatca aagactgaca atgttaactt
    10261 tgattattta tttgccgcta ttggtggtgg tggattaatt tcaggtatta gtacttactt
    10321 taaaacctat tcacctacta cgaaaattat aggtgttgaa ccttcaggtg caagtagtat
    10381 gtatgaatct gttgtcgtaa ataatcaggt agtcacattg cctaatatcg ataaatttgt
    10441 ggacggtgca tctgtagcta gagttggcga tattacattt gaaattgcaa aagaaaatgt
    10501 agatgattac gttcaagtag atgaaggtgc agtttgttct acgattttag acatgtattc
    10561 aaaacaagca attgtagcag aacctgctgg cgcattaagt gtgagtgctc ttgaaaacta
    10621 caaagaccac attaagggta aaactgttgt ttgtgtcatt agtggtggta ataatgatat
    10681 taatcgtatg aaggaaattg aagaacgttc tttattatat gaagaaatga aacattattt
    10741 tattttaaac ttccctcaac gccctggtgc attaagagaa tttgtaaatg atgttttagg
    10801 cccacaagac gatattacaa aatttgaata cttgaaaaaa tcttctcaaa atacaggtac
    10861 tgtcattatt ggtattcaac ttaaagatca tgatgattta atacaactca aacaacgtgt
    10921 aaatcatttc gatccttcca atatttatat taatgaaaat aagatgttat attcattgtt
    10981 aatttaacac atagtaagaa aaacagtcat aaattgattt ctaattgaaa tcatcttatg
    11041 actgcttttt attatacttt acatttctcg tttcgtcaga ttcaaacgtt ttcacttcgc
    11101 caagccattt ttctttgtgt ttgcttttat tttgacgttt tagacataaa aaaagagacc
    11161 ttgcggtctc aacttgcctg gtaacgttct actctagcgg aacgtaagtt tgctaccatc
    11221 gtcgctaaag acctttcttg acttgtgaca atcgcttgct tctttcctct ccttcggctc
    11281 tcgcttactc atttagctct actaaactcg ttgcgctctt ttcttgtttc gtcagattca
    11341 aacgttttca cttcgccaag ccatctttct ttgtgtttgc tttttactat gtaactttag
    11401 acataaaaaa agagaccttg cggtctcaat gcggctcatc gcatccattt tttgcctggc
    11461 aacgttctac tctagcggaa cgtaagttcg actaccatcg acgctaagga gcttaacttc
    11521 tgtgttcggc atgggaacag gtgtgacctc cttgctatag tcaccagaca tatgaatgta
    11581 a
//
LOCUS       DAMZKC010000083        11547 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.084, whole genome shotgun sequence.
ACCESSION   DAMZKC010000083 DAMZKC010000000
VERSION     DAMZKC010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11547
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.084"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..665
                     /gene="bshC"
                     /locus_tag="POB55_001602"
     CDS             <1..665
                     /gene="bshC"
                     /locus_tag="POB55_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bacillithiol biosynthesis BshC"
                     /protein_id="HDI6500565.1"
                     /translation="IAENQPELFSNNVVTRPLMEEWLFNTVAFVGGPSEIKYWAELKD
                     VFELFDVEMPIVMPRLRITYLNDRIEKLLSKYNIPLEKVLVDGVEGERSKFIREQASH
                     QFIEKVEGMIEQQRRLNKDLLDEVAGNQNNINLVNKNNEIHIQQYDYLLKRYLLNIER
                     ENDISMKQFREIQETLHPMGGLQERIWNPLQILNDFGTDVFKPSTYPPLSYTFDHIII
                     KP"
     gene            809..1240
                     /gene="mraZ"
                     /locus_tag="POB55_001603"
     CDS             809..1240
                     /gene="mraZ"
                     /locus_tag="POB55_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HDI6500566.1"
                     /translation="MFMGEYDHQLDTKGRMIIPSKFRYDLNERFIITRGLDKCLFGYT
                     LDEWQQIEEKMKTLPMTKKDARKFMRMFFSGAVEVELDKQGRINIPQNLRKYANLTKE
                     CTVIGVSNRIEIWDRETWNDFYEESEESFEDIAEDLIDFDF"
     gene            1256..2191
                     /gene="rsmH"
                     /locus_tag="POB55_001604"
     CDS             1256..2191
                     /gene="rsmH"
                     /locus_tag="POB55_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017724209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HDI6500567.1"
                     /translation="MFHHISVMLNETIDYLNVKENGVYIDCTLGGAGHALYLLNQLND
                     DGRLIAIDQDQTAIDNAKEVLKDHLHKVTFVHSNFRELTQILKDLNIEKVDGIYYDLG
                     VSSPQLDIPERGFSYHHDATLDMRMDQTQELTAYEIVNNWSYEALVKIFYRYGEEKFS
                     KQIARRIEAHREQQPITTTLELVDIIKEGIPAKARRKGGHPAKRVFQALRIAVNDELS
                     AFEDSIEQAIELVKVDGRISVITFHSLEDRLCKQVFQEYEKGPEVPRGLPVIPEAYTP
                     KLKRVNRKPITATEEDLDDNNRARSAKLRVAEILK"
     gene            2205..2606
                     /gene="ftsL"
                     /locus_tag="POB55_001605"
     CDS             2205..2606
                     /gene="ftsL"
                     /locus_tag="POB55_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HDI6500568.1"
                     /translation="MAVEKVYQPYDEQVYNSIPKQQPQTKPEKKTVSRKVVVQLTKFE
                     KVLYITLITVIAMLSIYMLSLKMDAYDTRGKIADLDYKIDKQSSENSALQSEIKKNSS
                     YERIYEKAKKQGMSLENDNVKVVRSNGEAKN"
     gene            2587..4821
                     /locus_tag="POB55_001606"
     CDS             2587..4821
                     /locus_tag="POB55_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDI6500569.1"
                     /translation="MAKQKIKIKKNKIGAVLLVGLFGLLFFILVLRISYIMITGHSNG
                     QDLVMKANEKYLVKNAQQPERGKIYDRNGKVLAEDVERYKLVAVIDKKASANSKKPRH
                     VVDKKETAKKLSTVINMKPEEIEKRLSQKKAFQIEFGRKGTNLTYQDKLKIEKMNLPG
                     ISLLPETERFYPNGNFASHLIGRAQKNPDTGELKGALGVEKIFDSYLSGSKGSLRYIH
                     DIWGYIAPNTKKEKQPKRGDDVHLTIDSNIQVFVEEALDGMVERYQPKDLFAVVMDAK
                     TGEILAYSQRPTFNPETGKDFGKKWANDLYQNTYEPGSTFKSYGLAAAIQEGAFDPDK
                     KYKSGHRDIMGSRISDWNRVGWGEIPMSLGFTYSSNTLMMHLQDLVGADKMKSWYERF
                     GFGKSTKGMFDGEAPGQIGWSNELQQKTSSFGQSTTVTPVQMLQAQSAFFNDGNMLKP
                     WFVNSVENPVSKRQFYKGQKQIAGKPITKDTAEKVEKQLDLVVNSKKSHAANYRIDGY
                     EVEGKTGTAQVAAPNGGGYVKGPNPYFVSFMGDAPKKNPKVIVYAGMSLAQKNDQEAY
                     ELGVSKAFKPIMENTLKYLNVGKSKDDTSNAEYSKVPDVEGQDKQKAIDNMSAKSLEP
                     VTIGSGTQIKAQSIKAGNKVLPHSKVLLLTDGDLTMPDMSGWTKEDVIAFENLTNIKV
                     NLKGSGFVSHQSISKGQKLTEKDKIDVEFSSENVDSNSTNNSDSNSDDKKKSDSKTDK
                     DKSD"
     gene            5113..6078
                     /locus_tag="POB55_001607"
     CDS             5113..6078
                     /locus_tag="POB55_001607"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HDI6500570.1"
                     /translation="MIFVYALLALVITFVLVPVLIPTLKRMKFGQSIREEGPQSHMKK
                     TGTPTMGGLTFLLSIVITSLVVIIFVDQANPIILLLFVTIGFGLIGFIDDYIIVVKKN
                     NQGLTSKQKFLAQIGIAIIFFVLSNVFHLVNFSTSIHIPFTNVAIPLSFAYVIFIVFW
                     QVGFSNAVNLTDGLDGLATGLSIIGFTMYAIMSFVLGETAIGIFCIIMLFALLGFLPY
                     NINPAKVFMGDTGSLALGGIFATISIMLNQELSLIFIGLVFVIETLSVMLQVASFKLT
                     GKRIFKMSPIHHHFELIGWSEWKVVTVFWAVGLISGLIGLWIGVH"
     gene            6080..7429
                     /locus_tag="POB55_001608"
     CDS             6080..7429
                     /locus_tag="POB55_001608"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HDI6500571.1"
                     /translation="MLNYTGLENKNVLVVGLAKSGYEAAKLLSKLGANVTVNDGKDLS
                     QDAHAKDLESMGISVVSGSHPLTLLDNNPIIVKNPGIPYTVSIIDEAVKRGLKILTEV
                     ELSYLISEAPIIAVTGTNGKTTVTSLIGDMFKKSRLTGRLSGNIGYVASKVAQEVKPT
                     DYLVTELSSFQLLGIEKYKPHIAIITNIYSAHLDYHENLENYQNAKKQIYKNQTEEDY
                     LICNYHQRQVIESEELKAKTLYFSTQQEVDGIYIKDGFIIYKGVRIINTEDLVLPGEH
                     NLENILAAVLACILAGVPIKAIIDSLTTFSGIEHRLQYVGTNRTNKYYNDSKATNTLA
                     TQFALNSFNQPIIWLCGGLDRGNEFDELIPYMENVRAMVVFGQTKAKFAKLGNSQGKS
                     VIEANNVEDAVDKVQDIIEPNDVVLLSPACASWDQYSTFEERGEKFIERFRAHLPSY"
     gene            7445..8764
                     /locus_tag="POB55_001609"
     CDS             7445..8764
                     /locus_tag="POB55_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HDI6500572.1"
                     /translation="MDDKTKNDQQESNEDKDELELFTRNTSKKRRQRKRSKATHFSNQ
                     NKDDTSQQADFDEEIYLINKDFKKEQSNDENNDSASSRANNNNIDDSTDSNIENEDYR
                     YNQEIDDQNESNGIAFDNEQPQSAPKEQNGDSNDEETVTKKERKSKVTQLKPLTLEEK
                     RKLRRKRQKRIQYSVITILVLLIAVILIYMFSPLSKIAHVNINGNNHVSTSKINKVLG
                     VKNDSRMYTFSKKNAINDLEENPLIKSVEIHKQLPNTLNVDITENEIIALVKYKGKYL
                     PLLENGKLLKGSNDVKINDAPVMDGFKGTKEDDMIKALSEMTPEVRRYIAEVTYAPSK
                     NKQSRIELFTTDGLQVIGDISTISKKMKYYPQMSQSLSRDSSGKLKTRGYIDLSVGAS
                     FIPYRGNTSSQSESDKNVTKSSQEENQAKEELQSVLNKINKQSSKNN"
     gene            8870..10282
                     /gene="ftsA"
                     /locus_tag="POB55_001610"
     CDS             8870..10282
                     /gene="ftsA"
                     /locus_tag="POB55_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HDI6500573.1"
                     /translation="MEEHYYVSIDIGSSSVKTIVGEKFHNGINVIGTGQTYTSGIKNG
                     LIDDFDIARQAIKDTIKKASIASGVDIKEVFLKLPIIGTEVYDESNEIDFYEDTEING
                     SHIEKVLEGIREKNDVQETEVINVFPIRFIVDKENEVSDPKELIARHSLKVEAGVIAI
                     QKSILINMIKCVEACGVDVLDVYSDAYNYGSILTATEKELGACVIDIGEDVTQVAFYE
                     RGELVDADSIEMAGRDITDDIAQGLNTSYETAEKVKHQYGHAFYDSASDQDIFTVEQV
                     DSDETVQYTQKDLSDFIEARVEEIFFEVFDVLQDLGLTKVNGGFIVTGGSANLLGVKE
                     LLSDMVSEKVRIHTPSQMGIRKPEFSSAISTISSSIAFDELLDYVTINYHDSEETEED
                     VIDVKDKDNESKLGGFDWFKRKTNKKDTHENEVESTDEEIYQSEDNHQEHKQNHEHVQ
                     DKDKDKEESKFKKLMKSLFE"
     gene            10315..11487
                     /gene="ftsZ"
                     /locus_tag="POB55_001611"
     CDS             10315..11487
                     /gene="ftsZ"
                     /locus_tag="POB55_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HDI6500574.1"
                     /translation="MLEFEQGFNHLATLKVIGVGGGGNNAVNRMIDHGMNNVEFIAIN
                     TDGQALNLSKAESKIQIGEKLTRGLGAGANPEIGKKAAEESREQIEDAIQGADMVFVT
                     SGMGGGTGTGAAPVVAKIAKEMGALTVGVVTRPFSFEGRKRQTQAAAGVEAMKAAVDT
                     LIVIPNDRLLDIVDKSTPMMEAFKEADNVLRQGVQGISDLIAVSGEVNLDFADVKTIM
                     SNQGSALMGIGVSSGENRAVEAAKKAISSPLLETSIVGAQGVLMNITGGESLSLFEAQ
                     EAADIVQDAADEDVNMIFGTVINPELQDEIVVTVIATGFDDKPTSHGRKSGSTGFGTS
                     VNTSSNATSKDESFTSNSSNAQATDSVSERTHTTKEDDIPSFIRNREERRSRRTRR"
BASE COUNT         4292 a         1570 c         2271 g         3414 t
ORIGIN      
        1 atattgcgga aaatcaacct gaattatttt ctaataatgt agtgacaaga ccattaatgg
       61 aagaatggtt atttaacacg gtggcatttg ttggaggacc gagtgaaatt aagtactggg
      121 ctgaactaaa agatgtattt gaactatttg atgttgaaat gcctatcgtg atgccaaggc
      181 ttagaattac ttatttaaat gaccgtatag aaaaattact ttcgaaatac aatattccat
      241 tagaaaaagt gttagtcgat ggtgttgaag gagaaagaag taagtttatt agagaacaag
      301 catcacatca atttattgaa aaggtagaag gtatgattga acaacagcgt cgtctaaaca
      361 aagacttatt agatgaagtg gcggggaatc aaaataatat taaccttgtg aataaaaata
      421 atgaaattca tatacaacag tatgattatt tgttaaaacg ttatctttta aacattgaaa
      481 gagaaaacga catcagtatg aagcaattta gagaaattca agaaacactc catccaatgg
      541 gaggattaca agaaagaata tggaatccac ttcaaatttt gaatgatttt gggacagatg
      601 tgttcaagcc ctccacctat ccaccacttt cttacacttt tgatcatatt attataaaac
      661 cttaatatac caagggttta gcccgattta tcttaatgat aaatcgggca tttttttgtt
      721 ttttaaaata aatttcacaa attttgtata aatagtggtg gatagtgggg agatgtggta
      781 aattatatat aaggtgaggt gataaaaaat gttcatggga gaatacgatc atcaattaga
      841 tacaaaagga cgtatgatta taccgtccaa gtttcgttat gacttaaatg agcgttttat
      901 tatcacaaga ggccttgata aatgtttatt cggttacact ctagacgaat ggcaacagat
      961 tgaagagaaa atgaaaacct tacctatgac aaaaaaagac gcacgtaagt ttatgcgtat
     1021 gttcttctct ggtgctgttg aagtagaact tgataagcaa gggcgtatta acatccctca
     1081 aaacttgagg aaatacgcta atttaactaa agaatgtaca gtaatcggtg tttcaaatcg
     1141 tattgagatt tgggatagag aaacttggaa tgatttctat gaagaatctg aagaaagttt
     1201 cgaagatatt gctgaagatt taatagattt tgatttttaa aatggaggaa ttgaagtgtt
     1261 tcatcatatc agcgttatgt taaacgaaac cattgattat ttaaatgtaa aagaaaatgg
     1321 tgtgtacatt gactgtacgc taggtggagc gggacatgcc ctttatttac taaatcaatt
     1381 aaatgacgac ggaagattaa tagcaatcga tcaagaccaa actgcaattg ataatgctaa
     1441 agaggtatta aaggatcatt tgcataaggt gacttttgtt catagcaact tccgtgaatt
     1501 aactcaaata ttaaaagact taaacattga aaaagtagat ggaatttatt acgacttggg
     1561 tgtttcaagc ccacaactcg acattccaga acgaggattc agttatcacc atgacgcaac
     1621 attagacatg cgtatggacc aaacacaaga actaacagca tatgaaattg ttaacaattg
     1681 gtcatatgaa gcgttagtga agatttttta tcgctatggc gaggagaaat tttcaaaaca
     1741 gatagctcga agaatcgaag cacatcgcga acaacaacca ataacaacaa cattagaatt
     1801 agttgacatt ataaaagaag gtattcctgc aaaagcaaga agaaaaggcg gacatcctgc
     1861 aaaacgagta tttcaagcac tacgaattgc agtaaacgat gaattgtcag cttttgaaga
     1921 ttcaatagaa caagcgattg aattagtgaa agtagatggc aggatttcgg taatcacttt
     1981 ccattcttta gaagatcgtt tatgtaaaca ggtgttccaa gaatatgaaa aaggtccaga
     2041 ggtaccaaga ggattaccag ttataccaga agcatataca cctaagttaa agcgtgttaa
     2101 tcgtaaaccg attaccgcta cagaagaaga tttagatgac aataacagag cacgaagcgc
     2161 gaaattacgt gtagctgaaa tacttaaata aggagcaatt tataatggct gtagaaaaag
     2221 tgtaccaacc atatgacgaa caagtttata atagtatacc gaagcaacaa ccacaaacta
     2281 agcccgaaaa gaagactgtt tcgagaaaag tggttgtaca attaactaaa tttgaaaaag
     2341 ttttatacat aactttgatt actgtaattg ctatgttaag tatttatatg ctatctttaa
     2401 aaatggatgc gtatgatacg cgaggaaaga ttgcagattt agattataaa atagataaac
     2461 aatcaagtga aaacagtgct ttacaatctg aaatcaaaaa gaattcttct tatgaacgca
     2521 tatacgaaaa ggctaagaaa caggggatga gccttgagaa cgataatgta aaggtagtgc
     2581 gtagtaatgg cgaagcaaaa aattaaaatt aaaaaaaata aaataggggc agtcctactt
     2641 gttggtttat tcggactgct cttttttata ttggttttaa gaatttcata tatcatgatt
     2701 actggacatt ctaatggtca agatttagtc atgaaggcaa atgaaaagta tttagttaag
     2761 aatgcacaac aaccagaacg aggaaagata tatgatcgta atggtaaagt gctagcagaa
     2821 gatgtagaaa gatataaact tgttgcagta atagataaaa aggcgagtgc caattctaaa
     2881 aaacctaggc atgtagttga taaaaaagag actgcaaaga aattatctac agtcattaat
     2941 atgaagccag aggaaattga aaagagactt agtcaaaaga aagctttcca aattgaattt
     3001 ggacgcaaag gaacaaattt aacgtatcag gacaaattga aaatagagaa aatgaatttg
     3061 cctggtattt ctttattgcc tgaaacagaa cgcttttatc caaatggcaa ttttgcatca
     3121 cacttaattg gtagagctca gaaaaatccg gatactggtg aacttaaagg tgcacttgga
     3181 gttgaaaaga tttttgatag ttatttaagt ggatctaaag gatcattgag atatattcat
     3241 gatatttggg gatatatcgc accaaatact aaaaaagaga agcagcctaa acgtggtgat
     3301 gatgtccatt taacaatcga ttcaaatatt caagtatttg ttgaagaagc tttagatggc
     3361 atggttgaaa gataccagcc gaaagattta tttgcggttg tcatggatgc caaaactgga
     3421 gaaattttag catacagtca gcgaccaaca tttaatcctg aaactggtaa agactttggt
     3481 aaaaagtggg caaatgacct ttatcaaaac acatacgagc ctggatcaac atttaaatca
     3541 tatgggttag cagctgctat tcaagaaggt gcttttgatc ctgataagaa atataaatct
     3601 ggacatagag atattatggg ttcacgtatt tcagactgga atagagtcgg ttggggtgaa
     3661 atcccaatgt cactcggatt tacttattca tctaatacat tgatgatgca tttacaagat
     3721 ttagttggtg cagacaaaat gaaatcttgg tatgaacgat ttggatttgg aaaatcaact
     3781 aaaggtatgt ttgatggaga agcacctggt caaattggat ggagtaatga gttgcaacaa
     3841 aaaacgtcat catttggtca atcgacaaca gtaacacctg ttcaaatgtt acaagcgcaa
     3901 tcagcgttct ttaatgatgg taatatgtta aaaccatggt ttgtgaatag cgttgaaaat
     3961 cctgttagta aaagacaatt ttataaaggg caaaaacaaa tcgcaggcaa accaataaca
     4021 aaagatactg ctgaaaaagt tgaaaagcaa ttggatttag ttgtgaatag taagaagagt
     4081 cacgctgcaa actatcgtat tgatggttat gaggtcgaag gtaagactgg tacagcacaa
     4141 gtcgctgcac ctaatggtgg tggatacgtt aaaggtccaa acccatattt tgtaagtttt
     4201 atgggtgacg cgccgaagaa aaatcctaaa gttattgtat acgctggtat gagcttggca
     4261 caaaaaaatg accaagaagc ttatgaatta ggtgttagta aagcgtttaa accaataatg
     4321 gaaaatactt tgaaatattt aaatgtaggt aaatcaaaag atgacacatc taatgcagag
     4381 tatagtaaag tgccagatgt tgaaggtcaa gacaaacaaa aagctattga taatatgagt
     4441 gcaaaatcat tagaaccagt tactatcggt tctggcacac aaataaaagc acaatctata
     4501 aaagcaggga ataaagtctt acctcatagt aaagtactgt tattaacaga cggagactta
     4561 actatgcctg acatgtcagg atggacgaaa gaagatgtca ttgcttttga aaacctaaca
     4621 aatattaaag taaatttaaa aggtagcggt tttgtgtccc accaatcaat tagtaaggga
     4681 caaaaactta ctgaaaaaga taaaatagac gtagaatttt catcagagaa tgtagacagc
     4741 aattcgacga ataattctga ttcaaattca gatgataaga agaaatctga cagtaaaact
     4801 gacaaggata agtcggacta acaagaatgg ctacattaaa taagggtgta ttgtcactat
     4861 ttgtttttac taaagtaatg ctgtgataga aaacaacatc ttcatttaat ctttataaat
     4921 aagtgttgat agagaatttt aaggtagacg aggtcagatt aacttgtttg ttataggcaa
     4981 actttttata ctggcctctt atgccgtaaa aaagaaaaat tataaatttt gattacaact
     5041 ttgtttattt aattgaaaaa ttgataatat attagtggtt aaaaaagtct ggattaagaa
     5101 gaggagatag ttatgatttt tgtatatgcg ttattagcgc tagtgattac atttgttttg
     5161 gtacctgttt taatacctac attaaaaagg atgaaatttg gtcaaagtat tcgagaagaa
     5221 ggtccacaaa gccatatgaa gaagactggt acaccaacga tgggtggact aacatttcta
     5281 ttaagtattg tgataacgtc tttggtggtt attatatttg tggatcaagc taatccaatc
     5341 atactgttat tatttgtgac gattggtttt gggttaattg gttttataga tgattatatt
     5401 attgttgtta aaaagaataa ccaaggttta acaagtaaac agaagttttt ggcgcaaatt
     5461 ggtattgcga ttattttctt tgttttaagt aatgtgtttc atttggtgaa tttttctacg
     5521 agcatacata ttccatttac gaatgtagca atcccactat catttgcata tgttattttc
     5581 attgtttttt ggcaagtagg tttttctaat gcggtaaatt taacagatgg tttagatgga
     5641 ttagcaactg gactgtcaat tatcggattt acaatgtatg ccatcatgag ctttgtgtta
     5701 ggagaaacgg caattggtat tttctgtatc attatgttgt ttgcactttt aggattttta
     5761 ccatataaca ttaaccctgc taaagtgttt atgggagata caggtagctt agctttaggt
     5821 ggtatatttg ctacgatttc aatcatgctt aatcaggaat tatcattaat ttttataggt
     5881 ttagtattcg taattgaaac cttatctgtt atgttacaag tcgctagctt taaattgact
     5941 ggaaagcgta tatttaaaat gagtccgatt catcatcatt ttgaattgat aggatggagc
     6001 gaatggaaag tagttacagt attttgggct gttggtctga tttcaggttt aatcggttta
     6061 tggattggag tgcattaaga tgcttaatta tacagggtta gaaaataaaa atgtattagt
     6121 tgtcggtttg gcaaaaagtg gttatgaagc agctaaatta ttaagtaaat taggtgcgaa
     6181 tgtaactgtc aatgatggaa aagacttatc acaagatgct catgcaaaag atttagaatc
     6241 tatgggcatt tctgttgtaa gtggaagtca tccattaacg ttgcttgata ataatccaat
     6301 aattgttaaa aatcctggaa taccttatac agtatctatt attgatgaag cagtgaaacg
     6361 aggtttgaaa attttaacag aagttgagtt aagttatcta atctctgaag caccaatcat
     6421 agctgtaacg ggtacaaatg gtaaaacgac agttacttct ctaattggag atatgtttaa
     6481 aaaaagtcgc ttaactggaa gattatccgg caatattggt tatgttgcat ctaaagtagc
     6541 acaagaagta aagcctacag attatttagt tacagagttg tcgtcattcc agttacttgg
     6601 aatcgaaaag tataaaccac acattgctat aattactaac atttattcgg cgcatctaga
     6661 ttaccatgaa aatttagaaa actatcaaaa tgctaaaaag caaatatata aaaatcaaac
     6721 ggaagaagat tatttgattt gtaattatca tcaaagacaa gtgatagagt cggaagaatt
     6781 aaaagctaag acattgtatt tctcaactca acaagaagtt gatggtattt atattaaaga
     6841 tggttttatc atttataaag gtgttcgtat tattaacact gaagatctag tattgcctgg
     6901 tgaacataat ttagaaaata tattagcagc tgtgcttgct tgtattttag ctggtgtacc
     6961 tattaaagca attattgata gtttaactac attttcagga atagagcata gattgcaata
     7021 tgttggtact aatagaacta ataaatatta taatgattcc aaagcaacaa acacgctagc
     7081 aacacagttt gccttaaatt catttaatca accaatcatt tggttatgtg gtggtttgga
     7141 tcgagggaat gaatttgacg aactcattcc ttatatggaa aatgttcgcg cgatggttgt
     7201 attcggacaa acgaaagcta agtttgctaa actaggtaat agtcaaggga aatcggtcat
     7261 tgaagcgaac aatgtcgaag acgctgttga taaagtacaa gatattatag aaccaaatga
     7321 tgttgtatta ttgtcacctg cttgtgcgag ttgggatcaa tatagtactt ttgaagagcg
     7381 tggagagaaa tttattgaaa gattccgtgc ccatttacca tcttattaaa gggtgtgagt
     7441 attgatggat gataaaacga agaacgatca acaagaatca aatgaagata aagatgaatt
     7501 agaattattt acgaggaata catctaagaa aagacggcaa agaaaaagat caaaggctac
     7561 acatttttct aatcaaaata aagatgatac atctcaacaa gctgattttg atgaagaaat
     7621 ttacttgata aataaagact tcaaaaaaga acaaagcaat gatgaaaata atgattctgc
     7681 ttctagtcgt gcaaataata ataatatcga tgattctaca gactctaata ttgaaaatga
     7741 ggattataga tataatcaag aaattgacga ccaaaatgaa tcgaatggaa ttgcattcga
     7801 caacgaacaa cctcaatcag ctcctaaaga acaaaatggc gactcgaatg atgaggaaac
     7861 agtaacgaaa aaagaacgaa aaagtaaagt aacacaatta aagccattaa cacttgaaga
     7921 aaagcggaag ttaagacgta agcgacaaaa acgaatccaa tacagtgtta ttacaatatt
     7981 ggtattgttg attgctgtta tattaattta catgttttca ccacttagta aaattgcgca
     8041 tgtaaatata aatggaaata atcacgttag tacttcaaag ataaacaaag ttttaggtgt
     8101 taaaaatgat tcaaggatgt atacgtttag taaaaaaaat gctattaatg atctcgaaga
     8161 gaatccatta atcaaaagtg ttgagataca caagcaatta ccaaacacat taaacgtaga
     8221 tatcacagaa aatgaaatta ttgctttagt gaaatataaa ggtaaatatt tacctttatt
     8281 agaaaatggt aaattgctta aaggttcaaa tgatgtcaaa attaatgatg cacctgtcat
     8341 ggatggtttc aaaggtacaa aagaagatga tatgattaag gcgttatctg aaatgacacc
     8401 tgaagttaga cgatatattg ccgaagtgac atacgcccca agtaaaaaca aacaaagcag
     8461 aattgaattg tttacgacag atggacttca agtaatcggt gatatttcga cgatatctaa
     8521 gaaaatgaaa tattatccgc agatgtcaca atcattatca agggatagtt cgggtaaact
     8581 aaaaacacga ggctatattg atttatcagt cggtgcttca tttatcccat accgtggaaa
     8641 cacgtctagt caatcagaaa gcgataaaaa tgtgactaaa tcatctcaag aggaaaatca
     8701 agcaaaagaa gaattacaaa gcgttttaaa caaaattaac aaacaatcaa gtaagaataa
     8761 ttaaaaaatt ttgatattgt ctatgtttat agttcacaag ccattcaacg tattgtaaac
     8821 taaggatagt gtattttttt aatagtaatt tgtcaggagg tgcctatcta tggaagaaca
     8881 ttactacgta agtattgata ttggatcatc aagcgtaaaa acaatagtag gcgagaaatt
     8941 tcacaatggt ataaatgtga taggtacagg acaaacctac acgagcggta taaaaaatgg
     9001 tttaattgat gattttgata ttgcgcgaca agcaatcaaa gacacaatta aaaaggcatc
     9061 aatcgcttcg ggtgttgata ttaaagaagt tttcctgaaa ttacctatca ttggaacgga
     9121 agtttatgat gaatcaaatg aaatcgactt ttatgaggat acagaaatca acggttcaca
     9181 tatcgaaaaa gtattagaag gtattagaga aaaaaatgat gtgcaagaaa cagaagtaat
     9241 taatgtgttc ccgattcgtt ttatagtcga taaagaaaat gaggtttcag accctaaaga
     9301 attaattgcc agacattcat taaaggttga agcaggcgta attgctattc aaaaatcgat
     9361 tttaattaat atgattaaat gcgtagaagc atgtggtgtt gatgtattag atgtttactc
     9421 tgatgcatat aactatggtt caatcctaac agctactgaa aaagagttag gtgcatgtgt
     9481 cattgatatt ggtgaagacg ttacgcaagt tgctttttat gaacgcggtg aattagtaga
     9541 tgctgattct atcgaaatgg cagggcgtga tattacagac gatattgcac aaggattaaa
     9601 cacttcttat gaaactgctg aaaaagttaa acaccaatat ggtcatgcat tctatgattc
     9661 tgcttcagat caagatatct tcactgttga acaggttgat agtgatgaaa cagtacagta
     9721 tactcaaaaa gatttgagtg actttattga agcgcgtgta gaagaaatat tcttcgaagt
     9781 atttgatgtt ttacaagatt taggattaac aaaagtaaat ggtgggttta ttgtaactgg
     9841 tggatctgca aacttacttg gcgtaaaaga attattatca gatatggtaa gtgaaaaagt
     9901 tagaattcac acgccatcac aaatgggaat tagaaaacct gaattttctt cagcaatttc
     9961 tacaatttct agtagtatcg cttttgatga gttattagat tatgttacaa ttaattatca
    10021 tgatagtgaa gaaactgaag aagatgttat tgatgtgaaa gacaaagata acgaatctaa
    10081 attaggcgga tttgattggt ttaaacgtaa aacaaacaaa aaagatactc atgaaaatga
    10141 agtagagtca acagatgaag aaatttatca atcagaagat aatcatcagg aacataaaca
    10201 gaatcatgaa catgttcaag acaaagataa agataaagaa gaaagtaaat tcaaaaaact
    10261 aatgaaatct ctatttgaat gattattggc caataaaact aggaggaaat ttaaatgtta
    10321 gaatttgaac aaggatttaa tcatttagcg actttaaagg tcattggtgt aggtggtggc
    10381 ggtaacaacg ccgtaaaccg aatgattgac cacggaatga ataatgttga atttatcgct
    10441 atcaacacag acggtcaagc tttaaactta tctaaagctg aatctaaaat ccaaatcggt
    10501 gaaaaattaa cacgtggttt aggagcagga gctaatcctg aaatcggtaa aaaagctgca
    10561 gaggaatctc gtgaacaaat tgaagatgca atccaaggtg cagacatggt atttgttact
    10621 tctggtatgg gtggcggaac tggtactggt gcagcaccag tcgttgctaa aattgcaaaa
    10681 gaaatgggcg cattaactgt tggtgttgta actcgtccat ttagttttga aggacgtaaa
    10741 cgtcaaactc aagctgctgc tggagtagaa gctatgaaag ctgcagtaga tacattaatc
    10801 gttataccaa atgaccgttt attagatatc gttgacaaat ctacgccaat gatggaagca
    10861 tttaaagaag ctgataacgt gttacgccaa ggtgtacaag gtatctcaga cttaatcgct
    10921 gtttctggtg aagtaaactt agactttgca gacgttaaga caattatgtc taaccaaggt
    10981 tctgcattaa tgggtattgg tgtttcttct ggtgaaaata gagcggtaga agctgctaaa
    11041 aaagcaatct cttctccatt acttgaaaca tctatcgttg gtgcacaagg tgtgcttatg
    11101 aatattactg gtggcgagtc attgtcatta tttgaagcac aagaggctgc tgatattgtc
    11161 caagatgctg cagatgaaga cgttaatatg attttcggta cagttattaa tcctgaatta
    11221 caagatgaga ttgttgtaac agttattgca actggttttg atgacaaacc aacatcacat
    11281 ggtcgtaaat ctggtagcac tggattcgga acaagcgtaa atacttctag caatgcaact
    11341 tctaaagatg aatcattcac ttcaaattca tcaaatgcac aagcaactga tagtgtaagt
    11401 gaaagaacac atacaactaa agaagatgat attcctagct tcattagaaa tagagaagaa
    11461 agacgttcaa gaagaacaag acgttaatcg gttaatatat atacacaaat aattcaacac
    11521 aaatcatcag ataacatatc tgatgat
//
LOCUS       DAMZKC010000084        11429 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.085, whole genome shotgun sequence.
ACCESSION   DAMZKC010000084 DAMZKC010000000
VERSION     DAMZKC010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11429
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.085"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(<1..192)
                     /locus_tag="POB55_001612"
     CDS             complement(<1..192)
                     /locus_tag="POB55_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500575.1"
                     /translation="MTEVKIKTISDRVYYTTTDLASDDYINLVMNLVIEDFLPVKDVF
                     NNEVWVKRDEIESFTFIKEA"
     gene            complement(189..395)
                     /locus_tag="POB55_001613"
     CDS             complement(189..395)
                     /locus_tag="POB55_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1381 domain-containing protein"
                     /protein_id="HDI6500576.1"
                     /translation="MTQYLVTTFKDSTGRKHTHITKAKSNQRFTVVEAESKEEAERKY
                     EAQVKRDAVIKVGQLFENIRECGK"
     gene            complement(432..968)
                     /locus_tag="POB55_001614"
     CDS             complement(432..968)
                     /locus_tag="POB55_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTPase"
                     /protein_id="HDI6500577.1"
                     /translation="MTNTLTIDQLQELLQIQKEFDDRIPTLNLRDSKIAYVVEFFEWF
                     NTLETFKNWKKKPGKPLDVQLDELADMLAFGLSIANQVGVSSEEIKEAIESSFKNSEF
                     HNMFNFKDKEFAQDAVVSTPQIIFKEFYPDQLAIVIAIDIAFNLYSIDQLIDAYKKKM
                     KRNHERQDGTADAGKGYV"
     gene            complement(961..1209)
                     /locus_tag="POB55_001615"
     CDS             complement(961..1209)
                     /locus_tag="POB55_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1024 family protein"
                     /protein_id="HDI6500578.1"
                     /translation="MNNREQIEQSIISASAYNGNDTEGLLKEIEDVYKKAQAFDEILE
                     GLPNAMQDALKEDIGLDEAVGIMTGQVVYKYEEDQEND"
     gene            complement(1224..1466)
                     /locus_tag="POB55_001616"
     CDS             complement(1224..1466)
                     /locus_tag="POB55_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500579.1"
                     /translation="MTDSARKERLNQFFGSKRYLYQDNERVAHTHVVNGTYYFHGHIV
                     PGWQSVKKTFDTAEELEIYIKQHGLEYEEQKQLTLF"
     gene            complement(1481..1681)
                     /locus_tag="POB55_001617"
     CDS             complement(1481..1681)
                     /locus_tag="POB55_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500580.1"
                     /translation="MATQKQVDYVMSLQEQLELEDCEKYTDEQVKAMSHKEVSNVIEN
                     YKTSIRNEELYYECMSFGLPNC"
     gene            complement(1684..1941)
                     /locus_tag="POB55_001618"
     CDS             complement(1684..1941)
                     /locus_tag="POB55_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3310 domain-containing protein"
                     /protein_id="HDI6500581.1"
                     /translation="MSIISNRKVDMNETQDNVKQPAHYTYGDIEIIDFIEQVTAQYPP
                     QLAFAIGNAIKYLSRAPLKNGHEDLAKAKFYVDRVFDLWEG"
     gene            complement(1941..2342)
                     /locus_tag="POB55_001619"
     CDS             complement(1941..2342)
                     /locus_tag="POB55_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PVL family protein"
                     /protein_id="HDI6500582.1"
                     /translation="MARRKVIRVRIKGKLMTLREVSEKYHISPELLRYRYKHKMRGDE
                     LLCGRKDSKSKDEVEYMKSQIKDEEKEREKIRKKAILNLYQRNVRAEYEEERKRRLRP
                     WLYDGTPQKHSRDPYWFDVTYNQMFKKWSEA"
     gene            complement(2342..2527)
                     /locus_tag="POB55_001620"
     CDS             complement(2342..2527)
                     /locus_tag="POB55_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3113 family protein"
                     /protein_id="HDI6500583.1"
                     /translation="MQHQAYINASVDIRIPTEVESVNYNQIDKEKENLADYLFNNPGE
                     LLKYNVINIKVLDLEVE"
     gene            complement(2540..4492)
                     /locus_tag="POB55_001621"
     CDS             complement(2540..4492)
                     /locus_tag="POB55_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HDI6500584.1"
                     /translation="MNIDIETYSSNDISKCGAYKYTEAEDFEILIIAYSIDGGAISAI
                     DMTKVDNEPFHADFETFKIALFDPAVKKYAFNANFERTCLAKHFNKQMPPEEWICTMV
                     NSMRIGLPASLDKVGEVLRLQNQKDKAGKNLIRYFSIPCKPTKVNGGRTRNLPEHDLE
                     KWQQFIDYCIRDVEVEMTIAHKIKDFPVTAIEQAYWVFDQHINDRGIKLSKSLMLGAN
                     VLDKQSKEELLNQAKHITGLENPNSPTQLLAWLKDDQGLDIPNLQKKTVQEYLKEATG
                     KAKKMLEIRLQMSKTSVKKYNKMHDMMCSDERVRGLFQFYGAGTGRWAGRGVQLQNLT
                     KHYISDTELEIARDLIKEQRFDDLDLLLNVHPQDLLSQLVRTTFTAEEGNELAVSDFS
                     AIEARVIAWYAKEQWRLDVFNTHGKIYEASASQMFNVPVESITKGDPLRQKGKVSELA
                     LGYQGGAGALKAMGALEMGIEENELQGLVDSWRNANPNIVNFWKACQEAAINTVKSRK
                     THHTHGVRFYMKKGFLMIELPSGRALAYPKASVGENSWGSQVVEFMGLDLNRKWSKLK
                     TYGGKLVENIVQATARDLLAISIARLEASGFKIVGHVHDEVIVEIPRGSNGLKEIETI
                     MNKPVDWAKGLNLNSDGFTSPFYMKD"
     gene            complement(4560..5117)
                     /locus_tag="POB55_001622"
     CDS             complement(4560..5117)
                     /locus_tag="POB55_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HDI6500585.1"
                     /translation="MKAKVLNKTKVITGKVRASYAHIFEPHSMQEGQESKYSISLIIP
                     KSDTSTIKAIEQAIEAAKEEGKVSKFGGKVPANLKLPLRDGDTEREDDVNYQDAYFIN
                     ASSKQAPGIIDQNKIRLTDSGTVVSGDYIRASINLFPFNTNGNKGIAVGLNNIQLVEK
                     GEPLGGASAAEDDFDELDTDDEDFL"
     gene            complement(5143..6309)
                     /locus_tag="POB55_001623"
     CDS             complement(5143..6309)
                     /locus_tag="POB55_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HDI6500586.1"
                     /translation="MKLDHSNRAHAKLSASGAKQWLNCPPSIKASEGIADKSTVFAEE
                     GTFAHELSELYFSLKYEGLTQFEFNKAFQNYKRNQYYSEELREYVEEYVANVEEKYNE
                     ALSRDNDVIALFETKLDLGKYVPESFGTGDVIIFSGGVLEIIDLKYGKGIEVSAIDNP
                     QLRLYGLGAYELLSLMYDIHTIRMTIIQPRIDNFSTEELPISRLLQWGADFVKPLARL
                     AYNGGGEFKAGSHCRFCKINHSCRTRAEYMQNVPQKPPHLLSDEEIAELLYKLPDIKK
                     WADEVEQYALNQAKENDKNYPGWKLVEGRSRRMITDTKAMLEKLVEAGYKPEDITETK
                     LLSITNLEKLIGKKAFSKITEGFIEKPQGKLTLATESDKRPAIKQSAEDDFDKL"
     gene            complement(6306..6668)
                     /locus_tag="POB55_001624"
     CDS             complement(6306..6668)
                     /locus_tag="POB55_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500587.1"
                     /translation="MAILEDIFEELKLLNNNLRVLNTELSTVDSSIVQEKVKEAPMPK
                     EETAQLESIEEVKETSADLTKDYVLSVGKEFLKKADTSDKKEFRNKLNELGADKLSTI
                     KEEHYEKIVDFMNARINA"
     gene            complement(6682..6942)
                     /locus_tag="POB55_001625"
     CDS             complement(6682..6942)
                     /locus_tag="POB55_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1108 family protein"
                     /protein_id="HDI6500588.1"
                     /translation="MYYKFGEIKNKIISFNGFEFKVSAMKKHDGISIQIKDMNNIPLK
                     SFHVVDLSELYFATDAMRDVINEWIEENTDEQDRLINLVMKW"
     gene            complement(6947..7249)
                     /locus_tag="POB55_001626"
     CDS             complement(6947..7249)
                     /locus_tag="POB55_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2482 family protein"
                     /protein_id="HDI6500589.1"
                     /translation="MTKKYKDMTQEEVKDLLSEKSGELYELAKEIKGESKFDILLFSS
                     IGVIDGDYLAGSSSVIGHTFDLASLLDSTKSYKDIVNVLQMCKSQKFHGIDDNKEG"
     gene            complement(7242..7559)
                     /locus_tag="POB55_001627"
     CDS             complement(7242..7559)
                     /locus_tag="POB55_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500590.1"
                     /translation="MAENIKTEQHYYTKDFSGYRNEEDNFVANQELTVTITLNEYRKL
                     IEIKAVKDKEEDTYRGKYFAEERKNEKLEKENIKLKNKIYELQNEEDNEEDEENEEEV
                     NND"
     gene            complement(7652..7813)
                     /locus_tag="POB55_001628"
     CDS             complement(7652..7813)
                     /locus_tag="POB55_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1270 domain-containing protein"
                     /protein_id="HDI6500591.1"
                     /translation="MSDTYKSYLIAVLCFTVLAIVLMPLLYFTTAWSIAGFASIATFI
                     YYKEYFYEE"
     gene            complement(7825..8088)
                     /locus_tag="POB55_001629"
     CDS             complement(7825..8088)
                     /locus_tag="POB55_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI6500592.1"
                     /translation="MPKIIVPPTPENTYRGEEKFVKKLYATPTQIHQLFGVCRSTVYN
                     WLKYYRKDNLGVENLYIDYSPTGTLINISKLEEYLIRKHKKWY"
     gene            complement(8113..8328)
                     /locus_tag="POB55_001630"
     CDS             complement(8113..8328)
                     /locus_tag="POB55_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500593.1"
                     /translation="MNKKSEGLDIIIPRIFRRDHASVESLTENERRLRKEILGSIKKG
                     DYSYLEINKVFYALDRELQYRANNNKL"
     gene            8375..8614
                     /locus_tag="POB55_001631"
     CDS             8375..8614
                     /locus_tag="POB55_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500594.1"
                     /translation="MISIHKRLLTQYLDKEIVTSLDLHLINGEVIKVQEHIKDAESKT
                     LHIIHPKDRVVSLDHVLYFDINVKGEKNNDSPYPS"
     gene            complement(8611..8787)
                     /locus_tag="POB55_001632"
     CDS             complement(8611..8787)
                     /locus_tag="POB55_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500595.1"
                     /translation="MNEEKLKMILLLLEDIPRDEWNRLVNEVNNQYSYQADKVGLASD
                     NCQQIANNYKHYGF"
     gene            complement(8871..9062)
                     /locus_tag="POB55_001633"
     CDS             complement(8871..9062)
                     /locus_tag="POB55_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001115061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI6500596.1"
                     /translation="MPEQLSVRKWRLVRDLKQQEVADILGVNAKTVGHWEKDDTNLSN
                     VTVYALAKLYDIEVDQIKV"
     gene            9223..9552
                     /locus_tag="POB55_001634"
     CDS             9223..9552
                     /locus_tag="POB55_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI6500597.1"
                     /translation="MENNKVRKILSENLQELMNDKNIDQRELAEAIGVSQPTVSNWIQ
                     QTKYPRIKRIQQLADYFNVPKSRITESKKDIHQETIAAHFDKEGLTEEEIEEVNRFIE
                     WVRNRDK"
     gene            9565..10026
                     /locus_tag="POB55_001635"
     CDS             9565..10026
                     /locus_tag="POB55_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000524999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HDI6500598.1"
                     /translation="MGLYEELCINNEKIKIEETDQLPNFQPGCYMNGKIYIRRNLSEV
                     RKAEVLYEELAHHKLTYGNILDQTKWINRKFENYAHRHGFTSAVPLHEIVEAHNYGVR
                     NLYELSEYLQLSESYILEAIEQYKKIYGIGTHYGEYSITFEPLRVFKYKEI"
     gene            10044..10478
                     /locus_tag="POB55_001636"
     CDS             10044..10478
                     /locus_tag="POB55_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016170817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500599.1"
                     /translation="MKKVIGLLLVSTLALTACGEKEKPKKEENKKSQTQKHKDSKPKT
                     QQEKMKKVEDKNPPNNSIQNNSNNQNQSQNNQLNNNSDPSNNTPANINENDSQNTNLN
                     DEYVVSPGWTKDEQAKAFEEYKKGKEEEARAGASAVPGANIN"
     gene            10507..10902
                     /locus_tag="POB55_001637"
     CDS             10507..10902
                     /locus_tag="POB55_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500600.1"
                     /translation="METNKTIDLMNYVEFPKRYTEAKGKLVAQPITTINSARRVENED
                     MTVCYILDQDDDVMDFIFDRDIITVYCPENGTATDEYFCEIIFNSDDTFTLKRLSNYV
                     TIKDRSYPMSKINDVNITGKVVRLFRDFK"
     gene            10992..11138
                     /locus_tag="POB55_001638"
     CDS             10992..11138
                     /locus_tag="POB55_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500601.1"
                     /translation="MNGGENFMVDKNKKQETTRSNPLNKSFEKSGASEKLKSTLSEKA
                     KKKD"
     gene            complement(11135..>11429)
                     /locus_tag="POB55_001639"
     CDS             complement(11135..>11429)
                     /locus_tag="POB55_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDI6500602.1"
                     /translation="RKFMKLDCISLLDKRDKLFNNNGKPVFIVIKDFENRIIEEGELK
                     TYNSAGSDFDLLEVERQDFKVSDLPSNDELYIKHTLVDLKQQIKLDLYLMNEY"
BASE COUNT         3447 a         2177 c         1633 g         4172 t
ORIGIN      
        1 tgcctcctta ataaatgtaa atgattcaat ctcatctctt ttaacccata cttcattgtt
       61 gaacacatct ttgaccggaa gaaaatcctc aatcactaga ttcataacaa gattaatata
      121 atcgtcagaa gctagatctg ttgttgtgta ataaactcta tctgaaatag ttttaatttt
      181 aacctccgtc atttcccaca ctcccttata ttttcaaata actgacccac tttaataact
      241 gcatctcttt taacttgtgc ctcgtatttg cgctcagctt cttctttact ctctgcctca
      301 acaactgtaa acctttgatt gcttttagct ttagttatgt gtgtatgctt gcgtcctgtt
      361 gaatctttga atgttgtgac taagtattgc gtcacttccc caaaacctcc ttgactcgat
      421 ctaagatgtc tttacacgta tccttttcct gcgtctgctg ttccatcttg tctttcatga
      481 ttccttttca ttttcttttt gtatgcgtca atgagctggt cgatagaata taagttgaaa
      541 gctatgtcta tcgctattac aattgccaat tggtcaggat aaaattcttt gaatattatc
      601 tgtggtgtgc taacaactgc gtcttgagca aattctttat ctttaaaatt aaacatgttg
      661 tgaaattcac tatttttaaa acttgattca atcgcttctt ttatctcttc tgatgacact
      721 cctacttgat tcgcaatact caatccaaac gccaacatgt ccgctaattc atctaactga
      781 acatctaacg gcttacctgg tttcttcttc cagttcttaa acgtttccaa tgtattaaac
      841 cattcaaaga attcaactac atatgctatt ttgctatctc gtaagttcag cgttggtatt
      901 ctatcgtcga actccttttg tatttgtaat aactcttgta actgatcaat tgttaatgta
      961 ttagtcattt tcctgatcct cctcatattt atagaccact tgccccgtca taatccctac
     1021 tgcttcatca agaccaatat cttctttgag tgcatcttgc atagcattag gtaaaccttc
     1081 aagtatttca tcaaacgctt gcgctttctt atacacgtct tcaatctctt ttagtaatcc
     1141 ctctgtgtca ttgccgttat acgcactagc actgataatt gattgttcaa tttgttcgcg
     1201 gttattcatc atttccatct cctctaaaat aaagttagtt gcttctgttc ctcgtattcc
     1261 aaaccatgtt gctttatata tatttcgagc tcttcagcag tatcaaatgt ctttttaacg
     1321 ctttgccaac ctggcacgat atgcccatga aagtaatacg tgccatttac tacatgagta
     1381 tgtgccactc gttcgttatc ctgatacaga tatcttttag agccgaaaaa ttggtttaag
     1441 cgttctttac gtgcgctatc tgtcatggtc gtcactcctt ttaacaatta ggcagtccaa
     1501 acgacatgca ttcgtaatat agttcttcat tccttatgct tgtcttatag ttctcaatca
     1561 cattgctaac ttctttatga ctcattgctt taacttgttc gtctgtatat ttttcgcagt
     1621 cttctaattc cagttgctcc tgtaatgaca ttacgtaatc aacttgtttt tgcgttgcca
     1681 tcgttacccc tcccacaagt caaacactct atcgacgtaa aacttcgcct ttgctaaatc
     1741 ctcatgacca ttctttaacg gtgctctaga caagtatttg attgcattac ctattgcgaa
     1801 tgctaattgt ggtggatact gtgccgtaac ttgttcgatg aaatctataa tttcaatgtc
     1861 gccgtatgtg taatgcgcag gttgtttaac attgtcttgt gtttcattca tatctacttt
     1921 tctgttactg attatgctca ttatgcttca ctccatttct tgaacatttg gttataagtg
     1981 acatcgaacc agtacggatc acgtgaatgt ttttgaggcg ttccatcata aagccatggt
     2041 ctcaatcttc tctttctttc ttcttcatat tccgctctca catttcgttg gtataggttc
     2101 aaaatcgctt tttttctgat tttttctctc tctttttctt catcttttat ttgactcttc
     2161 atatattcaa cttcatcttt agattttgag tcttttcttc cacacaataa ttcatcgccg
     2221 cgcattttat gtttgtatct gtatctaaga agttctggag atatatgata tttttctgaa
     2281 acttctctca atgtcattag ttttcctttg atacgcactc ttataacttt tcttctagcc
     2341 atcattccac ctctaaatct aaaaccttga tatttataac gttatatttt aatagttcac
     2401 ctggattatt aaataaatag tccgccaaat tctctttttc tttatcaatc tgattgtaat
     2461 taacactttc gacttctgta ggaattctaa tgtcaacaga agcattgata taagcttgat
     2521 gttgcattca atcacactcc taatccttca tataaaacgg agaagtaaat ccgtcactat
     2581 tcaaattcaa tccttttgcc caatcgacag gcttattcat gatagtttcg atttccttaa
     2641 gtccatttga acctctaggt atttctacaa ttacttcatc atggacatgt ccaactattt
     2701 taaaacctga tgcttcaagc ctagctatag aaatcgcaag taaatccctt gcagttgctt
     2761 gaacaatatt ctcgactaac ttcccaccat acgtttttaa ctttgaccat ttacggttaa
     2821 gatctaagcc cataaattca acaacttgac taccccaact attttcacca actgaagctt
     2881 ttggataagc taaagctctt ccactaggca gttcaatcat tagaaaacct tttttcatat
     2941 aaaatctaac tccatgcgta tgatgcgtct ttcgggattt cacagtatta attgcagcct
     3001 cttggcaagc cttccaaaaa ttaactatgt taggatttgc gttacgccaa ctatcaacta
     3061 aaccttgtaa ttcattttct tcaatgccca tttccaatgc gcccatcgct tttaaagctc
     3121 cagcgccacc ttgataacct aaagctaatt cggacacttt tcccttttgt ctgagagggt
     3181 cgcctttagt tatgctttct accgggacat taaacatttg agaagccgat gcttcatata
     3241 tctttccgtg tgtgttgaac acatctaaac gccattgttc ttttgcatac catgctatga
     3301 ctcttgcctc tattgcagaa aaatcactta ctgctagttc attaccttct tcagcagtaa
     3361 atgtcgtcct aactaattga cttaataagt cttgaggatg aacattgagt aataaatcta
     3421 aatcgtcaaa acgttgttct ttaataagat ctcttgctat ttctaattca gtatctgaaa
     3481 tataatgctt tgttaaattc tgaagttgta cacctctacc tgcccatctt ccagtaccgg
     3541 caccgtaaaa ttgaaacaga cctcttaccc gttcatcact gcacatcatg tcatgcattt
     3601 tgttgtattt tttcacactg gttttagaca tttgcaatct aatttctagc atttttttag
     3661 cttttcctgt tgcttctttt aagtactcct gaaccgtttt cttttgtaaa ttaggtatat
     3721 ctaatccttg gtcatccttt aaccaagcca ataattgtgt aggactatta ggattttcta
     3781 aacctgttat atgtttagct tgattaagca attcttcttt actctgctta tcgagcacat
     3841 tagctcctaa catcaatgat ttagaaagct taatacctct gtcgtttata tgttggtcaa
     3901 aaacccaata tgcttgttca attgcagtta ctggaaagtc tttaatttta tgagcaatcg
     3961 tcatttctac ttctacatct cgaatacagt aatctataaa ttgttgccat ttttcaagat
     4021 catgttcagg caagtttctt gttcttcctc cattaacttt tgttggttta caaggtatag
     4081 agaaataacg aattaaattt ttacctgctt tatctttttg gttttgtagt cttaaaactt
     4141 ctccaacttt atcaagcgaa gcaggtaagc caatacgcat tgaattaacc attgtgcaaa
     4201 tccattcttc aggtggcatc tgtttattaa aatgtttagc aagacaagtt ctttcgaaat
     4261 tagcattgaa tgcatacttt tttacagcag gatcaaaaag agcaatttta aacgtctcaa
     4321 aatcagcgtg gaaaggctca ttatctactt tagtcatgtc aatcgcacta atcgctccac
     4381 catctattga ataagctata attaaaattt cgaaatcttc agcttctgtg tatttatagg
     4441 caccacattt cgaaatatca ttactgctat atgtttcaat atctatattc ataaatctca
     4501 aattcttgac acctcaattt ctttaaaatt aaagtggggc taaaacccca cctattgact
     4561 tataagaaat cctcatcatc agtgtctaat tcatcgaaat catcttctgc tgcacttgca
     4621 ccgccaagag gttcgccttt ttctacaagt tgaatgttgt tcaatccaac tgcgataccc
     4681 ttattaccat ttgtgttgaa aggaaataga ttaattgaag ctctaatata atcaccactt
     4741 acaacagttc cagaatccgt taatctaatt ttgttttggt caataatacc aggtgcttgt
     4801 ttgcttgatg cgttaataaa ataagcgtct tgataattga catcatcttc tctttcagta
     4861 tctccatcac gtaatggaag tttcagattt gcaggaactt tgcctccaaa cttactaact
     4921 tttccttctt ctttagcagc ttctatagct tgttcaatgg cttttatcgt acttgtatct
     4981 gatttaggaa tgattaaact gattgaatac tttgattctt gcccttcttg catactgtga
     5041 ggttcaaaaa tatgtgcata tgatgctctt acttttcctg taatcacttt agttttattt
     5101 aatacttttg ctttcatgtt tatataccgt ccttttaaat ttttatagtt tgtcaaaatc
     5161 atcttcagca gattgcttta tagctggtcg tttatccgac tcggtagcaa gtgttaattt
     5221 accttgcggc ttttctataa aaccctctgt aattttagaa aatgcttttt taccaattaa
     5281 tttttctaaa ttcgtaatgc taagtaactt ggtttctgta atatcttcag gtttataacc
     5341 cgcttcaact aacttttcaa gcattgcttt tgtatcagtt atcattcttc gtgaacgacc
     5401 ttctacaagc ttccaaccag gatagttttt atcattttct ttcgcttgat ttagtgcata
     5461 ttgttctact tcatcagccc attttttgat atcaggcagt ttatataaaa gttctgcaat
     5521 ctcttcatca cttaacaaat gtggtggctt ttgaggcaca ttttgcatgt attctgcacg
     5581 tgttctacat gaatgattta tcttacagaa tctacaatga ctacctgctt taaactctcc
     5641 tccaccgtta taagcaagtc tggctaatgg tttaacaaaa tcggctcccc attgaagtaa
     5701 tcttgatatt ggtaactctt cagtagaaaa gttatctatt cgaggttgta tgatagtcat
     5761 gcgaattgta tgaatgtcat acattaaact aagcagttca tatgcgccca agccatataa
     5821 tctaagttga ggattatcta tagctgaaac ttcaatgcct ttaccatatt taaggtcaat
     5881 aatttcaagt acaccacctg aaaatataat gacatcacca gttccaaaag attcagggac
     5941 gtatttacct aaatctaatt ttgtttcaaa taaagctatt acatcattat ccctactcaa
     6001 agcttcgtta tatttttctt ctacattagc tacatactct tccacatatt cacgcaattc
     6061 ttcgctgtaa tattgatttc tcttataatt ttgaaaagcc ttattaaact caaactgtgt
     6121 tagcccttca tatttaaggc tgaaatataa ctcacttaac tcatgggcga atgtaccttc
     6181 ttcggcaaaa actgtacttt tatctgcaat accttcactt gccttaatac tcggtgggca
     6241 gtttagccat tgttttgctc cacttgcact aagctttgca tgagctctat ttgagtgatc
     6301 tagcttcatg catttattct cgcattcata aaatcaacaa ttttttcata atgctcttct
     6361 ttgatagtag atagcttatc cgcaccaagt tcgttaagtt tatttctaaa ttctttctta
     6421 tcagaagtat ctgctttttt aaggaactct tttcctactg ataaaacata atctttagtt
     6481 aaatcagcag aagtttcctt aacttcttca attgattcca gttgagctgt ttcttctttt
     6541 ggcattggtg cttctttaac tttctcttgt acaattgatg aatctactgt tgatagttca
     6601 gtgtttaaca cacgtaaatt gttatttaat agttttaatt cttcaaaaat atcttctaat
     6661 attgccattg attaaatcct cctaccattt catgactaag ttaattagtc tgtcctgttc
     6721 atctgtgttc tcttcaatcc attcgtttat aacgtcacgc attgcatccg tcgcaaaata
     6781 tagttcgctt aaatctacaa catgaaatga tttaagtgga atattattca tatccttgat
     6841 ttgtatactg ataccgtcat gttttttcat cgcagacact ttaaattcaa acccgttaaa
     6901 gctgataatt ttatttttta tctcaccaaa tttgtaatac attgttttag ccctccttgt
     6961 tatcatcaat accgtgaaat ttttgtgatt tacacatttg gagaacattg acaatgtctt
     7021 tataactctt agtgctatcc aataaggaag caagatcgaa agtatgacca atcacagaac
     7081 ttgaacctgc taaataatct ccgtcgataa ctcctattga tgagaaaagc aaaatatcaa
     7141 atttactttc tcccttaatt tctttcgcta attcatacaa ttctccgctt ttttcagata
     7201 ataagtcttt tacttcttcc tgagtcatgt ctttatattt tttagtcatt gttgacttcc
     7261 tcctcgtttt cttcgtcctc ctcgttatct tcttcgtttt gtaattcata aattttgttt
     7321 tttagtttta tattttcttt ttccaatttt tcgttttttc tttcttccgc aaaatactta
     7381 cctctgtaag tatcttcttc tttatcttta acagccttta tttcaataag ttttctgtac
     7441 tcgttcaatg tgattgttac tgtcaattct tgatttgcta caaagttatc ttcttcattt
     7501 ctgtatcctg agaaatcttt agtgtaataa tgttgttcag ttttaatatt ttcagccata
     7561 gttgactacc tccgtatatt ttgatttaat taagttgtat attttgataa atgtttgtca
     7621 ctgttacttg ttgacgcaag tagcagtttt tttattcttc ataaaagtat tctttgtagt
     7681 atatgaatgt tgcgatactt gcgaatcccg caattgacca tgctgtagtg aagtacagca
     7741 atggcataag cacaatcgct aagactgtga agcacagtac tgctattaag tagcttttat
     7801 atgtgtcgct catttaatat cctcctaata ccatttttta tgctttctga tcaaatactc
     7861 ttccaattta gaaatattaa tcagagtgcc tgttggtgaa taatcaatgt ataaattttc
     7921 tacacctaaa ttatctttgc ggtaatattt caaccagttg tatactgtac ttctacatac
     7981 tccaaacaat tgatggattt gtgtaggtgt tgcgtataac tttttcacaa atttttcttc
     8041 gcctctatat gtgttttctg gtgttggtgg tactatgatt tttggcatct ctatcactcc
     8101 tttcgataaa tgttaaagtt tgttattatt cgctctgtat tgaagttctc tatctaatgc
     8161 atagaaaact ttgtttattt ctaagtagct gtaatcacct tttttaatac ttcctaatat
     8221 ttcctttctt agtcgacgtt cattttctgt taaagattct actgacgcat gatctcttct
     8281 gaaaatcctt ggtatgatta tgtctaaccc ttctgatttt ttgttcattt gttgttccac
     8341 ctttcgtgta taatgttgtt atcaacctaa ggaggtgata agtatacata agagattgct
     8401 cactcaatat ttagataaag aaatcgttac ttctttagat ttacatttaa ttaatggtga
     8461 agttattaaa gtacaagaac atataaaaga tgctgaaagc aaaactctac acatcattca
     8521 tccaaaagat agagttgtca gtttagatca tgttttgtat ttcgacatta acgttaaagg
     8581 tgaaaagaat aacgatagtc cttatccatc ttaaaatcca tagtgcttat agttatttgc
     8641 tatttgttga cagttatcac tggcaagtcc taccttgtca gcttggtaac tgtattgatt
     8701 atttacttca tttactaatc gattccattc gtctcttggt atatcttcaa gaagtaagag
     8761 aatcatcttt aatttttctt cgttcatttg ttgttcctcc ttttaagttg tttatgtcca
     8821 tttgttacat tgcctaaaag ttataaaaag ttatacgaag gtcaaaaaat ttagaccttt
     8881 atctggtcta cctcaatatc atataacttt gctaaagcgt aaactgtaac attacttaaa
     8941 ttagtgtcat ccttttccca atgaccaact gtctttgcat tgacgcccaa tatatctgct
     9001 acttcttgct gtttcaagtc ccttacaagt ctccattttc ttacggataa ttgttccggc
     9061 atgtcgtcca cctccctttc acacgtataa ctttatatta ctttgtatta cttgtcaaca
     9121 gataaagtta tatttcttca taaaaagtta taaaaagtat tgtaaagtaa tgtcggttat
     9181 ggtaagttaa tattacatta agtaatatta aaggagacaa ctatggagaa taataaagtc
     9241 agaaaaattt tatctgaaaa ccttcaagaa cttatgaatg ataaaaatat tgatcagaga
     9301 gaacttgctg aagctattgg agtttctcaa cctacagtct ccaattggat tcaacaaact
     9361 aaatatccac gaattaaaag aattcaacaa cttgcagatt acttcaatgt accgaaatca
     9421 agaattactg aatcaaaaaa agatatacat caagaaacaa ttgctgctca ttttgataag
     9481 gagggattaa ctgaagaaga gattgaagaa gtaaatagat tcattgaatg ggttagaaat
     9541 agagacaaat aaagggtgtt tatattggga ttatacgaag aactttgcat aaataacgaa
     9601 aaaataaaga tagaagaaac tgaccagctt ccaaatttcc aacctggatg ctatatgaac
     9661 ggaaaaattt atataaggcg taatttatca gaagtacgta aagcagaagt gttatatgag
     9721 gaacttgccc accacaagtt gacgtatggc aacattttag accaaacaaa atggattaat
     9781 agaaaatttg aaaattatgc acatagacat ggttttactt cagctgtacc gctacatgaa
     9841 attgtagaag ctcacaacta cggtgttcgt aatttgtacg agttgtcaga gtatctgcag
     9901 ttgagtgaat catacatact agaagctata gaacaatata aaaagatata tggtattgga
     9961 actcactatg gcgagtattc tattacgttt gagccgttga gagtttttaa atataaggaa
    10021 atataaacaa aggagagata catatgaaaa aagtaatcgg actgctacta gtaagtacat
    10081 tagctttaac agcttgtggt gaaaaagaaa aaccaaaaaa agaagaaaat aaaaagtcac
    10141 aaacacaaaa acacaaagat agcaaaccaa aaacgcaaca agaaaaaatg aaaaaagttg
    10201 aagataaaaa tccacctaat aatagcatac aaaataattc aaacaatcaa aaccaatcac
    10261 aaaacaatca acttaataat aattcagatc catctaataa tactcctgca aatataaatg
    10321 aaaacgattc acaaaatact aatttaaatg atgagtatgt cgtttcgcct ggctggacta
    10381 aagatgaaca ggctaaagct tttgaagagt acaaaaaagg aaaagaagag gaagcaagag
    10441 ctggtgctag cgcagtacca ggagccaata ttaactaata aaacaatata agaaagaaga
    10501 gctaatatgg aaacaaataa aacaatcgat ttaatgaatt atgtggaatt tccaaaaaga
    10561 tacacagagg caaaaggcaa attagttgca caaccaataa ctactataaa tagcgcaaga
    10621 agagttgaaa atgaagatat gactgtttgc tacattttag atcaggatga tgatgtaatg
    10681 gactttatct ttgacagaga tataattact gtttactgtc ctgaaaacgg aactgcgact
    10741 gatgaatatt tttgtgaaat tatatttaac tcagatgaca catttaccct aaagcgatta
    10801 tctaattacg ttaccattaa agatagaagc tacccaatgt caaaaataaa tgacgtaaac
    10861 attacgggca aagtcgtcag attatttaga gattttaaat aaacttggct ttaattacga
    10921 ttaaaagtac ctatatagcg tgacgagaaa aaggattaaa aaaaatcaaa aaacgcctac
    10981 tagtgtagac gttgaatggt ggtgagaatt ttatggtaga taaaaacaaa aaacaagaaa
    11041 ctactcgtag taacccatta aacaaaagtt ttgaaaagtc aggcgccagc gaaaaattaa
    11101 aaagcacttt atcagaaaaa gctaagaaaa aagattagta ttcattcatt aaatataaat
    11161 ccaatttaat ttgttgttta aggtctacaa gcgtatgttt aatatacaat tcatcgtttg
    11221 acggtaaatc agatactttg aaatcttgtc gctcaacttc tagtaaatcg aaatcgctac
    11281 cagctgaatt ataggtttta agttcaccct cttcaatgat tctgttttca aagtctttta
    11341 taactataaa tactggttta ccgttgttat taaacaactt gtctcttttg tctaataagc
    11401 ttatacaatc caatttcata aactttctg
//
LOCUS       DAMZKC010000085        11343 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.086, whole genome shotgun sequence.
ACCESSION   DAMZKC010000085 DAMZKC010000000
VERSION     DAMZKC010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11343
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.086"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            23..970
                     /gene="hlgC"
                     /locus_tag="POB55_001640"
     CDS             23..970
                     /gene="hlgC"
                     /locus_tag="POB55_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component gamma-hemolysin HlgCB subunit C"
                     /protein_id="HDI6500603.1"
                     /translation="MLKNKILATTLSVSLLAPLANPLLENAKAANDTEDIGKGSDIEI
                     IKRTEDKTSNKWGVTQNIQFDFVKDKKYNKDALILKMQGFISSRTTYYNYKKTNHVKA
                     MRWPFQYNIGLKTNDKYVSLINYLPKNKIESTNVSQTLGYNIGGNFQSAPSLGGNGSF
                     NYSKSISYTQQNYVSEVEQQNSKSVLWGVKANSFATESGQKSAFDSDLFVGYKPHSKD
                     PRDYFVPDSELPPLVQSGFNPSFIATVSHEKGSSDTSEFEITYGRNMDVTHAIKRSTH
                     YGNSYLDGHRVHNAFVNRNYTVKYEVNWKTHEIKVKGQN"
     gene            972..1949
                     /gene="hlgB"
                     /locus_tag="POB55_001641"
     CDS             972..1949
                     /gene="hlgB"
                     /locus_tag="POB55_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component gamma-hemolysin HlgAB/HlgCB subunit
                     B"
                     /protein_id="HDI6500604.1"
                     /translation="MKMNKLVKSSVATSMALLLLSGTANAEGKITPVSVKKVDDKVTL
                     YKTTATADSDKFKISQILTFNFIKDKSYDKDTLVLKATGNINSGFVKPNPNDYDFSKL
                     YWGAKYNVSISSQSNDSVNVVDYAPKNQNEEFQVQNTLGYTFGGDISISNGLSGGLNG
                     NTAFSETINYKQESYRTTLSRNTNYKNVGWGVEAHKIMNNGWGPYGRDSFHPTYGNEL
                     FLAGRQSSAYAGQNFIAQHQMPLLSRSNFNPEFLSVLSHRQDGAKKSKITVTYQREMD
                     LYQIRWNGFYWAGANYKNFKTRTFKSTYEIDWENHKVKLLDTKETENNK"
     gene            complement(2001..2468)
                     /locus_tag="POB55_001642"
     CDS             complement(2001..2468)
                     /locus_tag="POB55_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HDI6500605.1"
                     /translation="MTSKDITQISVIAAILTILAALKIPSIIPGLDFQLSAPAALLIL
                     AFFGIKKYFLGGLLSSLLLLVFGVFNPINVIISIIFRVIAIAVVYLLKINVLSLVLAS
                     VLGSLVSRLLLSIILNLPVWVVLLNAIPGVIFTLIVAIPLYLTLRKRMAVLLR"
     gene            complement(2479..3171)
                     /locus_tag="POB55_001643"
     CDS             complement(2479..3171)
                     /locus_tag="POB55_001643"
                     /EC_number="6.2.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxyhexanoate--CoA ligase"
                     /protein_id="HDI6500606.1"
                     /translation="MYSIKMRSSNQDVHISGAETICEFDKIEQTVQRFYNKGFFHENG
                     QPDFLNIKIQKIMEPIQQIKALQIIEDDKANLQHLTQECGVTEQALNQGMTYIKNETV
                     YTGAIILSAISGKRLDSFGQRGIRATHFSFEDINNKGDLNERVTDALAIASCINAHPY
                     VKGELCVSDDLTYTTGYFASAKIGYHRLFDIKPVNTRYGGRIIFVDDRIDLNHYISFL
                     ESTPKQVVYERV"
     gene            complement(3182..4297)
                     /locus_tag="POB55_001644"
     CDS             complement(3182..4297)
                     /locus_tag="POB55_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HDI6500607.1"
                     /translation="MMFQEQIDLLQQKGLYRSLKSVGYIDRQYIEVENKRCTNYTSND
                     YLGLGQIAFDKDDFERFMRKYSYHLSSSRLISGSSTAYEEIETMLAGWLGYSACTILN
                     SGYDANLALFNIFKNTNCVVFSDQENHASIIDGIKLSGLEKVIYKHLDIADLEKRLEK
                     YPNQNIPKIIISDSAFSTNGDVVDIGQLVSLKHKYNATLILDVSHSFGIENYSNYQGV
                     DILTSSLSKACGAYGGVILSSNDVKDMLINHGRPLIYSSSLPIYNLYFIKRNIEKLIN
                     ADDRRTKLNSLSKYFNQKLKALNVNYNSSNSPIKFIEFDDIEAAENIHQTLLKHHVFT
                     SYLRYPTVTKPMLRISLSYFHTEQDIDRLFEILHQED"
     gene            complement(4275..5282)
                     /gene="bioB"
                     /locus_tag="POB55_001645"
     CDS             complement(4275..5282)
                     /gene="bioB"
                     /locus_tag="POB55_001645"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HDI6500608.1"
                     /translation="MNLAKRILQGEQLTKETVLKIYEDTNIDTLDLLNEAYILRKHYF
                     GKKVKLNMILNAKSGICPENCGYCGQSRDIKQKQRYALIPEEQIIDGAKVAHDNHIGT
                     YCIVMSGRGPSDKEVDHISNTVRTIKSQHPQLKICACLGLTNDEQAKKLKSAGVDRYN
                     HNINTSENYHDNVVTTHSYKDRTDTIELMKANNISPCSGVICGMGESNQDIVDMAFAL
                     KEMDADSIPINFLHPIKGTKFGSMDDLTPMKCLRIVALFRLINPTKEIRIAGGREVNL
                     RSLQPLALKAANSIFVGDYLITGGQPNQLDYDMINDLGFEIDYDTCENKENKNDVSRA
                     N"
     gene            complement(5284..6642)
                     /gene="bioA"
                     /locus_tag="POB55_001646"
     CDS             complement(5284..6642)
                     /gene="bioA"
                     /locus_tag="POB55_001646"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HDI6500609.1"
                     /translation="MNYTQQLKQKDSEYVWHPFTQMGVYSKEETIIIEKGKGSYLYDT
                     NGNKYLDGYASLWVNVHGHNNKYLNKVIKKQLNKIAHSTLLGSSNIPSIELAEKLIEI
                     TPSNLRKVFYSDTGSASVEIAIKMAYQYWKNIDREKYAKKNKFITLNHGYHGDTIGAV
                     SVGGIKTFHKIFKDLIFENIQVESPSFYRSNYDTENEMMTAILTNIEQILIERNDEIA
                     GFILEPLIQGATGLFVHPKGFLKEVEKLCKKYDVLLICDEVAVGFGRTGKMFACNHED
                     VQPDIMCLGKAITGGYLPLAATLTSQKIYNAFLSDSHGVNTFFHGHTYTGNQIVCTVA
                     LENIRLYEKRKLLSHIETTSSTLEKQLHALKRHRNVGDVRGRGLMFGVELVTDKDSKT
                     PLEIEKVERIVRKCKENGLMIRNLENVITFVPVLSMSNKEVKTMVRIFKKAVHNILDR
                     KC"
     gene            complement(6620..7306)
                     /gene="bioD"
                     /locus_tag="POB55_001647"
     CDS             complement(6620..7306)
                     /gene="bioD"
                     /locus_tag="POB55_001647"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HDI6500610.1"
                     /translation="MRIFITSTNTDVGKTYVTKHLYHALKTRGHRVCIFKPFQTEERQ
                     DGTFPDLEVFKNECDLSYDITSLYTFKQPVSPHLAFKMTDQIFLNKQRVLDKVKVLDK
                     EFDFILIEGAGGIAVPIYEGTDDFYMTKDLINDCADCVISVLPSKLGAISDAIVHQDY
                     VNQNVSASNFLIMNRYTDSYIEKDNQITIGKLTNKTVYTFEEHATYENFSEAFLKQLI
                     GVKNELHTTT"
     gene            complement(7361..7528)
                     /locus_tag="POB55_001648"
     CDS             complement(7361..7528)
                     /locus_tag="POB55_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500611.1"
                     /translation="MQLLILTDIFYRIAVYICPNSLRSLSHFNSKVKILPKFIVILRS
                     LNSCFLEKHAP"
     gene            complement(7759..9492)
                     /locus_tag="POB55_001649"
     CDS             complement(7759..9492)
                     /locus_tag="POB55_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6500612.1"
                     /translation="MFQITFKILNWIRPYKARMILGFSMSFLNAIFIALPIFLAAKIF
                     NNVLSHKPIYMKDILNVVIIMVLLVIGRFITAYFKSKSHESIAYEMSAKERLDIGDKL
                     KNVRLGYFNSHHSNELTTIVTTDLTFLENFAMKMVDVVVNGYILITVLILSLLVVSWQ
                     VSLLACIGVLLSFFAIQLLERKSRQNAPAYHNVQNQLVEKVLEVIRGIQVIKSFAKEN
                     TSLKSFNQAVNESKRINTKIEMQYIPFNLLHLLSLKVVSIMIVLVACLLYMNHSIDLP
                     TLIMISIFSFVIFDSVENINSAAHVLEMIDMTIDDIEKIKNAPELDENGKNLTIKNEN
                     IAFQNVNFSYDDKQVIKNVNFEIPTQTSTAIIGPSGSGKSTLCHLLLRFYDIDDGNIR
                     IDGVDIKDMTLSTLMSKISAVFQKVYLFNDTIENNILFGNPGATKEEIIRAAKQACCH
                     DFIMSLPEGYQTMLNEKGSNLSGGEKQRISIARAILKDAPIIILDEATASIDPENEQL
                     IQTAINELSKGKTVITIAHKLETIKNADQIIVLNEGEIIQKGSHDELIRKPGMYQDFI
                     RIKSKSAGWKL"
     gene            complement(9517..11280)
                     /locus_tag="POB55_001650"
     CDS             complement(9517..11280)
                     /locus_tag="POB55_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6500613.1"
                     /translation="MNNQTNWIKILSGFASDSKWKIMLSILLSIISVFSGLVPYWAVF
                     KIILMMINNAYTINSIMVYIFIALIAYISQVCCFGASTMLSHITAYEILSEIRKKLAQ
                     KLMRLPLGVVESKKIGELKNIFVDKVETIELPLAHMIPEVIGNLLLSAAIFLYIMLID
                     WRMASALLVTIPISIFAFKKVMSGFNETYAEQMKSNNYMNSAIVEFIEGIEVIKTFNQ
                     SQSSYKKYKDAVDNYKIHTLNWFKNTWGYMNLGASVLPSTFLGILPVGMYLISINQLN
                     YAEFFLCIVLSLGVVAPIKNFTNYVNHLKSIQYALTEVNQILSLEELVLSTKFKKPQH
                     YEIAFNNVGFSYNKDKDDLVFKHLSFTVPENNFTAIVGASGSGKSTIAKLISRYWDVT
                     SGEITIGGINIKDIESKQLNDLVGFVGQDNFLLNLTFKENIKLGNPEATDEAVEKAAK
                     LAQCHEFIEKLPDGYDTNVGTVGDKLSGGEKQRVTIARMILKDAPIIVLDEATAYVDP
                     DNEQKIQEALNVLTQDKTLIVIAHRLSTIQHADQIIVLGKQQILEKGSHHLLLKLNGN
                     YKKMWDTHMHTKDWGINTGHN"
BASE COUNT         3766 a         2061 c         1544 g         3972 t
ORIGIN      
        1 ttatagaaag aaagtgaaac ttatgcttaa aaataaaata ttagccacaa ctttatctgt
       61 gagcttactt gcccctcttg ccaatccgtt attagaaaat gctaaagctg ccaacgatac
      121 tgaagacatc ggtaaaggaa gcgatataga aattatcaaa aggacagaag ataaaacaag
      181 taataaatgg ggcgtgactc aaaatattca atttgatttt gtgaaggata aaaaatataa
      241 caaagatgct ttgatattaa agatgcaagg attcattagc tctagaacaa catattacaa
      301 ctataaaaaa actaatcatg ttaaagctat gcgatggcca ttccaatata atattggttt
      361 aaaaacaaat gataaatatg tttctttaat taattattta cctaaaaata aaattgaatc
      421 tacaaacgtg agtcagacat taggatacaa tatcggtggt aatttccaat cagccccatc
      481 actcggtggt aatggatcat ttaactattc taaatcgatt agctatacac aacaaaatta
      541 tgtaagtgaa gtagaacaac aaaactcaaa aagtgtttta tggggcgtca aagcgaattc
      601 attcgccact gaatcaggtc aaaaatcagc atttgatagc gatttatttg taggctacaa
      661 acctcatagt aaagatccta gagattattt cgttccagac agtgagttac cacctcttgt
      721 acaaagtgga tttaaccctt catttatcgc cacagtatct catgaaaaag gttcaagcga
      781 tacaagcgaa tttgaaatta cttacggaag aaacatggat gtcactcatg ccattaaaag
      841 atcaacgcat tatggcaaca gttatttaga cggacataga gtccataatg catttgtaaa
      901 tagaaactat actgtgaaat acgaggtcaa ttggaagact catgaaatca aggtgaaagg
      961 acagaattga tatgaaaatg aataaattag tcaaatcatc cgttgctaca tctatggcat
     1021 tattattact ttctggtact gctaatgctg aaggtaaaat aacaccagtc agcgtaaaaa
     1081 aagtcgatga caaagttact ttatacaaaa caacagccac agcagattct gataaattta
     1141 aaatttcaca gattttaaca tttaatttca tcaaagataa aagttatgat aaagatactt
     1201 tagtacttaa agctactggg aatattaact caggctttgt gaaacctaat cctaatgact
     1261 atgacttttc aaaattatat tggggagcta aatacaatgt atctataagc tcacaatcta
     1321 atgattcagt aaacgtcgtt gattatgcac caaaaaatca aaatgaagag tttcaagttc
     1381 aaaatacttt aggctataca tttggtggtg acattagtat ctctaatggt ttatctggtg
     1441 gacttaatgg aaatacagct ttttctgaaa caattaatta taaacaagaa agttacagaa
     1501 caacattaag tcgcaacaca aattataaaa atgttggctg gggagttgaa gcacataaaa
     1561 ttatgaataa tggttgggga ccttatggac gagatagctt ccacccaaca tatggtaatg
     1621 aactcttctt agctggcaga caaagcagtg catacgctgg ccaaaacttc atagcgcaac
     1681 accaaatgcc attattatct agaagtaact tcaatccaga atttttaagc gtactatcac
     1741 acagacaaga tggcgctaaa aaatctaaaa ttacagtaac ttatcaacgt gaaatggatt
     1801 tataccaaat tcgttggaat ggcttctact gggcaggcgc aaattataaa aactttaaaa
     1861 ctagaacatt taaatcaaca tatgaaattg attgggaaaa tcacaaagtg aaattgttag
     1921 atacaaaaga aactgaaaac aataaatagc tagtaaaaca cggtcgccaa cagtaattgt
     1981 gacgaccgtg ttttgattta ttatcttagt aagactgcca ttctttttct caatgtgaga
     2041 tataaaggaa tagctacaat taaagtgaat attacgcctg gaatcgcgtt taacaacact
     2101 acccacacag gtaaatttaa aataatagat aatagtagcc tagataccaa actgcctaat
     2161 acacttgcta aaactaatga tagtacattt attttcaata aataaacaac tgcaatagct
     2221 ataactctaa atataataga aataatcaca ttaattggat taaatacgcc aaatactagt
     2281 aataataggc tagataataa tccacctaaa aagtactttt taattccaaa gaaagctaat
     2341 atcaataatg ctgccggtgc agataattga aaatctaatc ctggtataat ggacggtatt
     2401 ttcaaagctg ccaaaatggt taaaatcgca gcaatgacac taatttgagt aatatctttt
     2461 gatgtcatac taaaacccct ataccctttc ataaacaact tgcttcggtg tgctttctaa
     2521 aaatgatatg taatgattta aatcaatacg atcgtccaca aatattattc tgcctccata
     2581 tctcgtatta actggtttaa tatcaaataa tcgatggtaa ccaattttag cagaggcaaa
     2641 ataacctgtc gtatacgtta agtcatctga cacacaaagt tctcctttga cataaggatg
     2701 cgcattgata caactagcaa ttgctaaggc atcagtcact ctttcattaa gatcaccttt
     2761 attatttata tcttcaaacg aaaaatgtgt tgccctaatc cccctttgtc caaatgaatc
     2821 taaacgttta ccagatatag cagatagaat aatagctcct gtataaaccg tttcattttt
     2881 aatatatgtc atcccttgat ttagcgcttg ttcagtgaca ccacattctt gtgttaaatg
     2941 ttggagattt gctttatcat cctcaataat ttgtaatgct tttatttgtt gaatcggttc
     3001 catgattttt tgtattttta tattgagaaa atccggttgc ccattttcat gaaaaaaacc
     3061 tttattataa aacctttgta ccgtctgttc tattttgtca aactcacaaa tcgtttcagc
     3121 accactaata tgaacatctt gattgctaga acgcattttt atactataca tgacgatcac
     3181 ctcaatcttc ttgatgcaaa atttcaaaca acctatctat atcttgttca gtatgaaaat
     3241 acgacaatga tattcttaac attggcttag tcacagttgg ataccttaaa taacttgtaa
     3301 acacatgatg ctttaataat gtttgatgaa tgttctcagc cgcttctatg tcatcaaact
     3361 caataaactt aatcggcgag tttgaactat tataattaac attgagtgct tttaactttt
     3421 ggttaaaata tttactcaaa ctatttaatt tagtgcgtct atcatcagca tttattaact
     3481 tttcaatgtt tctttttata aaatacaaat tataaattgg caaactactt gagtagatga
     3541 gtggtctacc gtgattaatt aacatatcct tcacatcatt tgaacttaaa atcacacccc
     3601 cgtatgcacc acatgcttta gataaactag aagtgagtat atctacacct tgataattcg
     3661 agtaattctc tattccaaaa ctatgtgaaa catcgagtat cagtgttgcg ttatatttat
     3721 gctttaatga gactaattga ccaatatcca caacgtcgcc attcgttgaa aatgcactat
     3781 cagatatgat tatttttggt atattttgat tagggtattt ctctaacctt ttttctaaat
     3841 cagcaatatc taaatgctta tatatcactt tttctaaacc acttaactta ataccgtcaa
     3901 taatactcgc atgattttct tgatctgaaa acacgacaca atttgtattt ttgaaaatat
     3961 taaataacgc caaattagca tcataaccac tatttaagat agtacatgca ctatatccga
     4021 gccaacctgc taacattgtt tcaatttctt cataagctgt cgaacttcca ctaattaatc
     4081 ttgaacttga taagtgataa ctatacttcc gcataaatct ttcgaaatca tccttatcaa
     4141 acgctatttg acctaatcct aaataatcat tagatgtata gttcgtacat ctcttatttt
     4201 ctacttcaat atactgtcta tctatatacc ctaccgattt aagcgaccga tataaccctt
     4261 tctgttgtaa taaatcaatt tgctcttgaa acatcattct tgttttcctt attttcacaa
     4321 gtgtcataat caatttcaaa gcccaaatca ttaatcatat cgtagtctaa ttggttcggt
     4381 tgcccaccag taattagata atcaccgaca aatattgaat tcgccgcttt taatgctaat
     4441 ggctgtaacg aacgtaagtt gacctctctt cctccagcaa tacgaatttc tttcgtagga
     4501 ttgattaatc ggaataatgc tacgattctt aaacatttca ttggtgttaa atcatccatg
     4561 cttccaaact ttgtgccttt gattggatgc aaaaaattaa tcggaatact gtcggcatcc
     4621 atttctttca aagcaaatgc catatcaaca atatcttgat tagattctcc cataccacaa
     4681 atcacgccag aacatggtga tatattattc gctttcatta gttctatcgt atctgttcta
     4741 tctttataac tatgcgttgt cacgacgtta tcatggtaat tttcacttgt attaatattg
     4801 tggttatatc tgtctacacc agctgactta agtttcttag cttgttcgtc attcgttaat
     4861 cctaaacatg cacagatttt tagttgcggg tgttgagatt taatcgttct tacagtatta
     4921 ctaatatgat caacttcttt atcgctcggt cctctaccac tcataacaat acaatatgtt
     4981 ccaatatgat tatcatgtgc cacctttgct ccatcgataa tttgttcctc tggaattaaa
     5041 gcatatcgct gtttttgttt aatatctcgt gattgtccac agtacccaca attctcagga
     5101 catataccac ttttagcatt taaaatcatg tttaatttta cttttttacc gaaataatgt
     5161 tttcttaaaa tgtacgcctc atttaataaa tctaaggtat caatattagt atcctcataa
     5221 attttcaata cagtctcttt tgttaattgt tccccttgta atatgcgttt agccaaattc
     5281 atattaacac ttcctatcta aaatgttatg tactgccttt ttaaaaatac gtaccatcgt
     5341 tttcacttct ttatttgaca tacttaacac tggcacaaac gtaatgacat tttctaaatt
     5401 tctaatcatt agcccgtttt ctttacattt acgtacaata cgttcaactt tttcaatttc
     5461 taacggcgtt ttactatctt tatctgtaac taattcaact ccaaacatta agcctcgtcc
     5521 tcttacatca ccaacatttc gatgacgctt cagcgcatgc aactgtttct caagtgttga
     5581 tgatgtcgtt tcaatatgtg acaataactt acgtttttca taaagtctta tattttctaa
     5641 tgcaaccgta caaacgattt gattcccggt gtatgtatga ccatggaaaa aggtattcac
     5701 accatgcgaa tcacttaaaa atgcattgta tattttttga gatgtcaatg tagctgcaag
     5761 tggtaagtag ccacctgtaa tcgccttacc taaacacata atatccggtt gaacatcttc
     5821 atgattgcat gcaaacatct ttccagttct cccaaaacca actgctacct catcacaaat
     5881 taataagaca tcgtattttt tgcacaattt ctcgacttct ttcaaaaagc ctttaggatg
     5941 aacaaacaag cctgtcgcac cttgaatcaa cggttccaat ataaaccctg cgatttcatc
     6001 atttctttca attagaattt gctctatatt cgttaaaata gctgtcatca tttcattttc
     6061 agtatcgtaa ttactgcgat agaaagatgg gctttctact tgaatattct caaatattaa
     6121 gtctttaaat attttatgaa aggtcttgat accaccaaca cttaccgcac caatcgtatc
     6181 cccatgataa ccgtgattta gcgttataaa cttgtttttc ttggcatatt tttctctatc
     6241 aatatttttc caatactgat atgccatctt tattgcgatt tcaacagacg cactgcctgt
     6301 atcagaataa aatacttttc ttagattact tggcgtgatt tcgattaatt tttccgcaag
     6361 ttctattgac ggaatatttg atgatcctag cagcgtagaa tgggcaattt tattgagttg
     6421 ctttttaatt accttattca agtatttgtt attatgacca tgcacattga cccacaacga
     6481 tgcataacca tctaaatatt tattgccatt cgtatcgtaa aggtaactac cctttccttt
     6541 ttcaatgatg attgtttctt ctttgctata tacacccatt tgtgtaaatg gatgccaaac
     6601 atattctgag tctttttgtt taagttgttg tgtgtaattc atttttaact cctattaatt
     6661 gttttaaaaa tgcttctgag aaattttcat acgtggcatg ttcttcaaat gtatagactg
     6721 ttttatttgt taattttcca atcgtaattt gattgtcttt ttcaatatag ctgtctgtat
     6781 agcgattcat tattaaaaaa ttactcgccg atacattctg attaacataa tcttggtgaa
     6841 caatggcatc gctaatagca cctaattttg atggcaacac actgatgaca caatctgcac
     6901 aatcattgat tagatcttta gtcatgtaga aatcatctgt accttcatat attggtacgg
     6961 caattccccc agcaccctca attaagataa aatcaaattc cttatctaaa acttttacct
     7021 tatctaatac acgctgctta tttagaaaaa tttgatctgt cattttaaat gcaaggtgtg
     7081 gtgatacagg ttgcttaaaa gtataaagtg acgttatgtc atagcttaaa tcacattcat
     7141 ttttaaatac ttctaaatct ggaaacgtcc cgtcttggcg ttcctcagtt tgaaatggtt
     7201 taaaaataca aactcgatga ccacgtgttt tcaaagcatg gtataaatgc tttgtaacat
     7261 aggttttgcc tacatcagta ttcgtacttg taataaaaat cctcataaaa aataagcacc
     7321 tcaatccgaa tgtaaacttt tataattaat aagtttacat ttaaggcgca tgtttttcaa
     7381 gaaaacatga atttaaactt ctcagtataa ctataaattt tggcaaaatt ttgaccttgc
     7441 tattgaaatg actaagactt cttaaggaat tcgggcagat ataaacagcg attcgataaa
     7501 atatgtcagt taaaattaat aattgcatta tttcttaaca aaaagacatc ctagctttac
     7561 catcttgtga aacaatccat taattgtaat attacgttga gcgccttgtt catcaatgct
     7621 ataattagtt acataataaa ttgaacatct aaatacacca aatcccctca ctattgccct
     7681 agtgagggga tttattttgc tgttgattat ctatcaccct ttttatttcc aaattctgat
     7741 tccatattaa actattattt ataatttcca gcctgctgat ttacttttta ttcttataaa
     7801 gtcttgatac attcctggtt ttcgaattaa ttcatcatga ctaccttttt gaattatttc
     7861 accttcattg agcactataa tctgatctgc atttttaata gtttcaagtt tatgtgcaat
     7921 tgtaattact gttttgcctt tacttaattc attaattgcc gtttgaatca gctgttcatt
     7981 ttcagggtca atacttgcag ttgcttcatc taaaataatt attggtgcat cttttaatat
     8041 cgccctagca atagaaatcc tttgcttttc tccgccagat aaattactac ctttttcatt
     8101 tagcattgtt tgatatccct caggtaatga catgatgaag tcgtggcaac atgcttgctt
     8161 cgctgcacga ataatttctt ctttcgttgc acctggattg ccaaacaata tgttattttc
     8221 aatcgtatca ttaaataaat acaccttttg aaatactgca ctaattttcg acattaacgt
     8281 acttaatgtc atatctttaa tatcaacacc atcgatgcga atatttccat catcgatatc
     8341 ataaaagcgc aagagtaagt gacataatgt agatttccca cttcctgaag gtccaattat
     8401 tgctgttgat gtttgtgtag gtatctcaaa attcacattc tttatcactt gtttatcatc
     8461 atatgaaaag ttcacgtttt gaaaagcgat attttcattt ttaatcgtca aattttttcc
     8521 attctcatcc agttctggag cattttttat cttttcaata tcatctatcg tcatatctat
     8581 catttcaagt acgtgtgcag cactattaat attttcaaca ctatcaaata tcacaaatga
     8641 aaaaattgaa atcataataa gggtaggtaa atcaatacta tgattcatat ataataagca
     8701 tgcaactaat acaatcatta ttgaaacaac ttttaaacta agtaaatgca ataaattgaa
     8761 tgggatatat tgcatttcta tttttgtatt tatacgttta ctttcattga ctgcttggtt
     8821 aaaactttta agactcgtat tttctttcgc gaatgatttt attacttgaa taccacgaat
     8881 aacctccaat accttttcca ctaattggtt ttgtacatta tgatacgctg gcgcattttg
     8941 tcgactcttt ctttctaata attgaatcgc aaaaaatgat agtaatacgc caatgcatgc
     9001 taataatgat acttgccacg aaactacaag tagagacaat atgagtactg taattaatat
     9061 gtatccatta acaacaacgt ccaccatttt catagcaaag ttttctaaaa aggttaaatc
     9121 tgttgttact attgttgtta actcatttga atgatgcgaa ttaaaataac ctaaccttac
     9181 atttttcaat ttatccccta tatctaaacg ttctttcgca ctcatttcat aagcgatgct
     9241 ctcatggctt ttgcttttga aatatgctgt aataaatcgt ccaatcacta ataaaaccat
     9301 gataattaca acattcaata tatctttcat ataaataggt ttatgagata gcacattatt
     9361 aaatattttt gcagctaaaa agataggtag cgcaataaaa atagcattta aaaatgacat
     9421 gctaaatcct aaaatcattc ttgctttata tggtcttatc cagtttaaaa ttttaaatgt
     9481 aatttgaaac atatttgacc tccttttctc attagtttaa ttatgtccag tattgattcc
     9541 ccaatctttt gtatgcatgt gcgtatccca cattttctta taattaccgt ttaattttag
     9601 caataagtga tgtgaccctt tttctaaaat ttgttgttta cctaaaacaa taatttgatc
     9661 tgcatgttga atcgtagata accgatgtgc aataacaatc aatgttttat cctgcgtcaa
     9721 cacatttaat gcttcttgaa ttttttgttc gttgtctgga tcaacgtaag cagtcgcttc
     9781 atctaataca atgataggcg catcttttaa tatcattctt gcaatagtga ctcgttgttt
     9841 ttcaccacca gacaatttat ctcccactgt accaacattc gtatcatatc catctggcaa
     9901 cttttcaata aattcatgac attgtgctaa cttggcagct ttttcaactg cctcatccgt
     9961 agcttctgga ttgccaagtt taatattttc tttaaaagta agatttaata aaaagttatc
    10021 ttgtccaaca aaaccaacta aatcgttaag ttgtttcgat tcaatatctt ttatatttat
    10081 accgccaatc gtaatttcac ctgaagtcac atcccagtat cgtgatataa gcttggcaat
    10141 ggttgactta ccgctaccag atgccccgac gatagctgtg aaattatttt ctggtactgt
    10201 aaatgataaa tgcttaaaga ccagatcgtc cttatctttg ttatatgaaa atccaacatt
    10261 attaaaagca atttcataat gttgaggctt cttaaatttt gttgacaata ctaactcttc
    10321 tagacttaat atttgattca cttcagttaa tgcgtattgt atagacttta aatgatttac
    10381 ataattagta aaattcttaa tcggtgctac tacacctaaa gataatacga tgcataggaa
    10441 aaattccgca tagtttaatt ggttgataga tatcaaatac atgccgaccg gtaaaatccc
    10501 taaaaatgtt gaaggtaata cactagctcc taaattcata tacccccatg tatttttaaa
    10561 ccaattcaaa gtgtgaatct tataattatc tactgcatct ttatattttt tatatgaact
    10621 ttgagattga ttaaatgttt taatcacttc aatgccttcg ataaactcta caatcgcgct
    10681 attcatataa ttattcgatt tcatttgttc agcatatgtc tcattaaatc cagacataac
    10741 ttttttaaaa gcgaaaattg aaattggtat cgttactaat aaggcactag ccatacgcca
    10801 atcaatgagc attatgtata aaaagatagc agctgacaaa agtaagtttc ctataacttc
    10861 aggaatcata tgtgctaaag gtaattctat tgtttcaacc ttatcgacaa atatattttt
    10921 taattcacct attttcttag attccactac gcctaaaggg agacgcatta atttttgagc
    10981 taatttttta cgaatttcag ataaaatttc atatgccgta atatgtgata gcatcgttga
    11041 cgctccaaaa caacacactt gtgaaatata agcgattaaa gcaataaaga tataaaccat
    11101 aatcgaatta atcgtatatg cattgttaat catcattaaa ataattttaa agactgccca
    11161 atatggaact aatccagaaa agacactgat gatagacaac aaaattgata acataatttt
    11221 ccatttacta tcagaagcaa atccgctgag tatctttatc caatttgtct gattgttcat
    11281 cgtatatgta actcctttca attaataatc taaattaagc cgcttatatt atttatttca
    11341 ctg
//
LOCUS       DAMZKC010000086        11241 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.087, whole genome shotgun sequence.
ACCESSION   DAMZKC010000086 DAMZKC010000000
VERSION     DAMZKC010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11241
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.087"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            391..2580
                     /locus_tag="POB55_001651"
     CDS             391..2580
                     /locus_tag="POB55_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HDI6500614.1"
                     /translation="MNNEYPYSADEVLHKAKSYLSADEYEYVLKSYHIAYEAHKGQFR
                     KNGLPYIMHPIQVAGILTEMRLDGPTIVAGFLHDVIEDTPYTFEDVKEMFNEEVARIV
                     DGVTKLKKVKYRSKEEQQAENHRKLFIAIAKDVRVILVKLADRLHNMRTLKAMPREKQ
                     IRISRETLEIYAPLAHRLGINTIKWELEDTALRYIDNVQYFRIVNLMKKKRSEREAYI
                     ETAIDRIRTEMDRMNIEGDINGRPKHIYSIYRKMMKQKKQFDQIFDLLAIRVIVNSIN
                     DCYAILGLVHTLWKPMPGRFKDYIAMPKQNLYQSLHTTVVGPNGDPLEIQIRTFDMHE
                     IAEHGVAAHWAYKEGKKVSEKDQTYQNKLNWLKELAEADHTSSDAQEFMETLKYDLQS
                     DKVYAFTPASDVIELPYGAVPIDFAYAIHSEVGNKMIGAKVNGKIVPIDYILQTGDIV
                     EIRTSKHSYGPSRDWLKIVKSSSAKGKIKSFFKKQDRSSNIEKGRMMVEVEIKEQGFR
                     VEDILTEKNIQVVNEKYNFANEDDLFAAVGFGGVTSLQIVNKLTERQRILDKQRALNE
                     AQEVTKSLPIKDNIITDSGVYVEGLENVLIKLSKCCNPIPGDDIVGYITKGHGIKVHR
                     TDCPNIKNETERLINVEWVKSKDATQKYQVDLEVTAYDRNGLLNEVLQAVSSTAGNLI
                     KVSGRSDIDKNAIINISVMVKNVNDVYRVVEKIKQLGDVYTVTRVWN"
     gene            2592..3044
                     /locus_tag="POB55_001652"
     CDS             2592..3044
                     /locus_tag="POB55_001652"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HDI6500615.1"
                     /translation="MKVVVQRVKEASVTNDTLNNQIKKGYCLLVGIGQNSTEQDADVI
                     AKKIANARLFEDDNNKLNFNIQQMNGEILSVSQFTLYADVKKGNRPGFSNSKNPDQAV
                     KIYEYFNDALRAYGLTVKTGEFGTHMNVSINNDGPVTIIYESQDGKIQ"
     gene            3041..3916
                     /locus_tag="POB55_001653"
     CDS             3041..3916
                     /locus_tag="POB55_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDI6500616.1"
                     /translation="MKKIEAWLSKKGLKNKRTLIVVIAFVLFIIFLFLLLNSNSEDSG
                     NITITENAELRTGPNAAYPVIYKVEKGDHFKKIGKVGKWIEVEDTSSNEKGWIAGWHT
                     NLDIVADNTKEKNPLQGKTIVLDPGHGGSDQGASSNTKYKSLEKDYTLKTAKELQRTL
                     EKEGATVKMTRTDDTYVSLENRDIKGDAYLSIHNDALESSNANGMTVYWYHDNQRALA
                     DTLDATIQKKGLLSNRGSRQENYQVLRQTKVPAVLLELGYISNPTDETMIKDQLHRQI
                     LEQAIVDGLKIYFSA"
     gene            4377..5639
                     /locus_tag="POB55_001654"
     CDS             4377..5639
                     /locus_tag="POB55_001654"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HDI6500617.1"
                     /translation="MIKIPRGTQDILPEDSKKWRYIENQLDELMTFYNYKEIRTPIFE
                     STDLFARGVGDSTDVVQKEMYTFKDKGDRSITLRPEGTAAVVRSYIEHKMQGNPNQPI
                     KLYYNGPMFRYERKQKGRYRQFNQFGVEAIGAENPSVDAEVLAMVMHIYQSFGLKHLK
                     LVINSVGDMASRKEYNEALVKHFEPVIHEFCSDCQSRLHTNPMRILDCKVDRDKEAIK
                     TAPRITDFLNEESKAYYEQVKAYLDDLGIPYIEDPNLVRGLDYYTHTAFELMMDNPNY
                     DGAITTLCGGGRYNGLLELLDGPSETGIGFALSIERLLLALEEEGIELDIEENLDLFI
                     VTMGDQADRYAVKLLNHLRHNGIKADKDYLQRKIKGQMKQADRLGAKFTIVIGDQELE
                     NNKIDVKNMTTGESETIELDALVEYFKK"
     gene            5655..7421
                     /gene="aspS"
                     /locus_tag="POB55_001655"
     CDS             5655..7421
                     /gene="aspS"
                     /locus_tag="POB55_001655"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HDI6500618.1"
                     /translation="MSKRTTYCGLVTEAFLGQEITLKGWVNNRRDLGGLIFVDLRDRE
                     GIVQVVFNPAFSEEALKIAETVRSEYVVEVQGTVTKRDPETVNPKIKTGQVEVQVTNI
                     KVINKSETPPFSINEENVNVDENIRLKYRYLDLRRQELAQTFKMRHQITRSIRQYLDD
                     EGFFDIETPVLTKSTPEGARDYLVPSRVHDGEFYALPQSPQLFKQLLMISGFDKYYQI
                     VKCFRDEDLRADRQPEFTQVDIEMSFVDQEDVMQMGEEMLKKVVKEVKGVEINGAFPR
                     MTYKEAMRRYGSDKPDTRFEMELIDVSQLGRDMDFKVFKDTVENDGEIKAIVAKGAAE
                     QYTRKDMDALTEFVNIYGAKGLAWVKVVEDGLTGPIGRFFETENVETLLTLTGAEAGD
                     LVMFVADKPNVVAQSLGALRVKLAKELGLIDETKLNFLWVTDWPLLEYDEDAKRYVAA
                     HHPFTSPKEADIAKLGTAPEEAEANAYDIVLNGYELGGGSIRIHDGELQEKMFEVLGF
                     TKEQAQEQFGFLLDAFKYGAPPHGGIALGLDRLVMLLTNRTNLRDTIAFPKTASATCL
                     LTNAPGEVSDKQLEELSLRIRH"
     gene            7499..7689
                     /gene="ssrS"
                     /locus_tag="POB55_001656"
     ncRNA           7499..7689
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="POB55_001656"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            7754..7882
                     /locus_tag="POB55_001657"
     CDS             7754..7882
                     /locus_tag="POB55_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500619.1"
                     /translation="MGFIAFNKCFLVEFWENNQSDNAKYKILSYYVLMYKSVKEVK"
     gene            7882..8655
                     /locus_tag="POB55_001658"
     CDS             7882..8655
                     /locus_tag="POB55_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HDI6500620.1"
                     /translation="MKHQFSRNELAIGQQGLDLLKQQTVVILGVGGVGSFAAEALART
                     NIGHIILIDKDDVDITNVNRQLHALTTTVGQSKVSLMEERIKLINPDCKVTPLHMFYT
                     EETYEEIFDNYDIDYFVDASDTIMYKVHLMKECLDRGIKVISSMGAANKTDPTRFTIS
                     DISKTHTDPMAKIIRQKLKKLGIRKGIPVVFSDESPIVIREDVKDIVGDKNAINRKGQ
                     MPPSSNAFVPSVVGLICASYVVNDVLKDIPVRRIKDKGQ"
     gene            complement(8816..10090)
                     /locus_tag="POB55_001659"
     CDS             complement(8816..10090)
                     /locus_tag="POB55_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HDI6500621.1"
                     /translation="MSTEPLASRMRPKNIDEIISQQHLVGPRGIIRRMVDTKKLTSMI
                     FYGPPGIGKTSIAKAISGSTQYKFRQLNAVTNTKKDMQLVVEEAKMSGQVILLLDEIH
                     RLDKAKQDFLLPHLENGKIVLIGATTSNPYHAINPAIRSRAQIFELYPLNDEDVRQAL
                     TRAIEDEENGLKTYQPKIDEDAMTYFSTQSQGDVRSALNALELAVLSADNDKDGYRHV
                     TLQDAKDCLQKGAFVSDKDGDMHYDVMSAFQKSIRGSDVNAALHYLARLIEAGDLPTI
                     VRRLLVISYEDIGLASPNAGQRTLAAIESAERLGLPEARIPLSQAVIELCLSPKSNSA
                     MSAIDSALSDIRNGHVGQIPNHLKDGHYQGAKDLGRSIGYKYPHQYVNGYVSQQYLPD
                     KLKNKIYYEPKTTSKSEQQLKEIYNNLLKQRP"
     gene            10175..10597
                     /locus_tag="POB55_001660"
     CDS             10175..10597
                     /locus_tag="POB55_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002458938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDI6500622.1"
                     /translation="MKISTKGRYGLTLMISLAKKEGQGCISLKSIAEENNLSDLYLEQ
                     LVGPLRNAGLIRSVRGAKGGYQLRVPAEEISAGDIIRLLEGPITFVESIESEPPAQKQ
                     LWIRMRDAVRDVLDNTTLKYLAEYVDTSEDLDGYMFYI"
     gene            10697..10879
                     /locus_tag="POB55_001661"
     CDS             10697..10879
                     /locus_tag="POB55_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDI6500623.1"
                     /translation="MADESKFDQFKGNVKETVGNVTDNKELEKEGQQDKATGKAKEVV
                     ENAKNKITDAIDKLKK"
     gene            10919..11065
                     /locus_tag="POB55_001662"
     CDS             10919..11065
                     /locus_tag="POB55_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500624.1"
                     /translation="MSFMDKAKDAVEKFKNSDNEQVKNVKDKINEYTGSNNEEKKENE
                     DKEK"
BASE COUNT         4050 a         1556 c         2187 g         3448 t
ORIGIN      
        1 aatgtataag aaccaatgac ttaattaaaa aagttgttta agttttctta acatgagatg
       61 ttaggatttt ttatttactg aaaatgttag atgattgagc attatacctt aataacatcg
      121 tttatttatt tcataaattg tagtatcata gaactaatat ttaaaaaatg aaacagtaga
      181 tttaggtcga atttttgtaa aagttttaaa agtaggaata gtatacaaat taaactcgct
      241 caagtaaaat taatattacg attaatgacg acaggataaa tatttatcgt cgacggacgt
      301 atgattggtg tgggacaaat actattcaac aagagtacct aaatcattgt ttaaggcgaa
      361 gtaataaata tgaatggggt gtatcatata atgaacaacg aatatccata tagtgcagat
      421 gaagttcttc acaaagcaaa atcatatttg tcagcagatg aatatgagta tgttttaaaa
      481 agctatcata ttgcttatga agcacataaa ggtcagttcc gaaaaaacgg attaccatac
      541 attatgcatc ctatacaagt tgcaggtatt ttaacagaaa tgcgattaga cggaccgacg
      601 attgtcgcag gttttttgca tgatgtaatt gaagatacac cgtatacatt tgaagatgta
      661 aaagaaatgt tcaatgaaga agttgctcga attgttgatg gtgtgacgaa gcttaaaaaa
      721 gtaaaatacc gctcaaaaga agaacaacaa gctgaaaatc atcgcaagtt atttattgcg
      781 attgccaaag atgtacgcgt aattttggtg aaattagcag acagattaca taatatgcgt
      841 accttgaaag ccatgccgcg tgaaaaacaa attagaattt ctcgagaaac attagaaatt
      901 tatgcacctt tagcacatcg tcttggtatt aatacaatta aatgggaact agaagatacg
      961 gctcttcgtt atattgataa tgtgcaatat tttagaatag tcaatttaat gaagaagaaa
     1021 cgtagtgaac gtgaagcgta tatcgaaacg gctattgata gaatacgtac tgaaatggac
     1081 cgaatgaata tcgaaggcga tataaatggt agacctaaac atatttacag tatttatcgg
     1141 aaaatgatga agcagaaaaa acaatttgat caaatttttg atttgttggc gatacgtgtt
     1201 attgtcaatt ctattaatga ttgttatgcg atacttgggt tggtgcatac gttatggaaa
     1261 ccgatgccag gacgttttaa agattatatt gcaatgccta aacaaaattt gtatcagtca
     1321 ttgcatacta cagtagtagg tccaaatgga gacccgctcg aaatccaaat acgaacgttt
     1381 gatatgcacg aaattgctga gcatggtgtt gcagcacact gggcttacaa agaaggtaaa
     1441 aaagtaagtg aaaaagatca aacttatcaa aataagttaa attggttaaa agaattagct
     1501 gaagcggatc atacatcgtc tgacgctcaa gaatttatgg aaaccttaaa atatgactta
     1561 cagagtgaca aagtatacgc atttacccca gcgagtgatg ttattgagtt gccatatggt
     1621 gctgtgccga ttgattttgc ttatgcgatt cacagtgaag taggtaataa gatgattggt
     1681 gccaaggtga atggcaaaat tgtaccaatt gactatattt tacaaacagg cgatattgtt
     1741 gaaatacgta ctagtaaaca ttcatatgga ccaagtcgtg attggttgaa aattgttaaa
     1801 tcgtctagtg ccaaaggtaa aattaaaagt ttcttcaaaa aacaagatcg ttcatctaat
     1861 attgaaaaag gccgaatgat ggttgaagtt gaaataaaag agcaaggatt tagagtcgaa
     1921 gatattttga cagagaaaaa tattcaggtt gttaatgaaa aatataactt tgcaaatgaa
     1981 gatgatttat tcgcagctgt aggatttggc ggcgtgacat ccttacagat tgttaataaa
     2041 ttaactgaaa gacaacgtat tttagataaa caacgtgctt taaatgaagc acaagaagtt
     2101 acgaaatcat tgcctattaa agacaacatc attactgata gtggtgtcta tgtagaaggt
     2161 ttagaaaatg tacttatcaa gttgtcaaaa tgttgtaatc ctataccagg tgatgatatt
     2221 gtaggttata tcaccaaagg tcacggtatt aaagtacatc gcactgattg cccaaatatt
     2281 aagaacgaaa ctgaacgact aattaatgtt gaatgggtaa aatcaaaaga cgcaactcaa
     2341 aaatatcagg ttgatttaga ggtaactgcg tatgaccgaa atggcttgtt gaatgaagta
     2401 ctacaagctg ttagctcgac agccggcaat ttaattaaag tttcaggacg ttcagatatt
     2461 gataaaaatg caataataaa tattagtgtc atggtgaaaa acgtgaatga tgtttatcgt
     2521 gtggtagaaa agatcaaaca acttggtgat gtttatacag taacaagagt ttggaactag
     2581 aggtgcaaaa tatgaaagta gttgtacaaa gagttaaaga agcatcggtg acgaatgata
     2641 cattaaataa tcaaatcaaa aaaggatatt gtttattagt cggtatcggt cagaactcta
     2701 cagagcaaga tgcagatgta attgcaaaga aaattgctaa tgcaagatta tttgaagatg
     2761 acaataataa attaaacttt aatatccaac aaatgaatgg tgaaatacta tcagtttcac
     2821 aatttactct ctatgcagat gtaaaaaaag gtaaccgtcc aggtttctca aattctaaaa
     2881 atcctgatca agcggtaaaa atttatgagt attttaatga tgcgctacga gcgtatggtc
     2941 ttactgtgaa aacaggtgaa tttggaacac acatgaatgt tagcataaat aatgatggtc
     3001 cagtcactat tatttatgaa agtcaggacg gcaaaattca atgaaaaaaa tagaggcatg
     3061 gttatctaaa aagggtctta aaaataaacg tactctaata gtagtgattg cctttgtctt
     3121 atttatcatc tttttatttt tattgctgaa tagcaatagt gaagatagtg ggaacatcac
     3181 gataactgaa aatgctgaat tacgtacagg tccaaacgct gcgtatccag tcatatataa
     3241 agttgagaaa ggtgaccatt ttaaaaagat tggtaaagta ggtaaatgga ttgaagttga
     3301 agatacatcc agtaatgaaa aaggttggat agctggatgg cacacaaatt tagatattgt
     3361 cgcggataat acgaaggaga aaaatccttt gcaaggtaaa acaatagtgc ttgatcctgg
     3421 tcatggaggt agtgaccagg gtgcttcaag caatactaaa tataaaagtt tagaaaaaga
     3481 ctatacgttg aaaacagcaa aagaattgca gcgtacttta gaaaaagaag gcgcaactgt
     3541 taagatgaca agaacagacg atacatatgt ttcactagaa aatcgtgata tcaaaggcga
     3601 tgcctatttg agtatacata atgatgcatt agaatcatct aatgcaaatg gaatgacagt
     3661 ttattggtat catgataatc aaagagcttt agcagatacg ttagacgcta cgattcagaa
     3721 gaaaggtcta ctttctaatc gcggttcaag acaagaaaat tatcaagtgt taagacaaac
     3781 aaaagttcct gctgttttat tagaattagg ttatattagt aacccaactg atgaaacgat
     3841 gattaaagat caattacata gacaaatttt agaacaagca attgttgatg gccttaaaat
     3901 ttatttttct gcgtagggct tgcaaaaata tgtgaaagta gttatcattg atattgaatt
     3961 ttataactaa aaccgttagt attcttgaaa tggtaaatga aataggtagc aatctaacta
     4021 agattgtgta ggaatataat ccatagactg aaagattatg ctgagtagtt tatatacatt
     4081 gaacacaaga agaggtgctt tatgaaaagt aaagccgtta aacgtacgtt aaacgttttg
     4141 agtgggttta tttaatgcac gcttataaaa agtaatgatg attacaatta ggcatgtttt
     4201 ttaaaccaat gagggtggca acacggatat tcgtcccttg tgcagtttca ggctgtgcaa
     4261 gggctttttc ttttgctttt aaaattatga tttatcatgc aaagttaagt gatcgtatat
     4321 agttttagtt ttaaaaaggt aattaaataa aatagtttgc cgagggagat gtcaaaatga
     4381 ttaaaatacc tagagggacg caggatattt tacctgaaga ttcaaagaaa tggcgttaca
     4441 ttgaaaatca attagatgaa ttaatgacat tttataatta taaagaaata agaacaccaa
     4501 tttttgaaag tacagatctt tttgcaagag gtgttggtga ttcaaccgat gtcgtacaaa
     4561 aagaaatgta tacatttaaa gataaaggcg atagaagtat tacattaaga cctgagggaa
     4621 cagctgcagt tgtgcgttca tatattgaac ataaaatgca aggtaatcca aaccaaccaa
     4681 ttaaacttta ttacaatgga ccgatgttta gatatgaacg taagcaaaaa gggcgctatc
     4741 gtcaatttaa tcaatttggt gtagaagcta ttggtgctga aaatcctagc gtagatgcag
     4801 aagtattagc tatggttatg catatttatc aatcatttgg attaaaacat ttaaagcttg
     4861 ttattaatag tgtaggggat atggcgtctc gaaaagaata taacgaagcg ttagtgaaac
     4921 actttgaacc agtaattcat gaattttgtt cagattgtca atcacgtttg catacaaatc
     4981 cgatgcgaat tttggattgt aaagttgacc gtgataaaga agcgattaag actgcaccta
     5041 gaatcactga tttcttaaat gaggaatcta aggcatatta tgaacaagta aaagcttatt
     5101 tagatgattt aggtattcca tatattgaag atcctaactt agttcgtgga ttggattatt
     5161 atacacatac agcatttgaa ttaatgatgg ataaccctaa ctatgatggt gccattacaa
     5221 cgctttgtgg tggtggccgt tataatggtt tattagaatt gctagatggt ccaagtgaaa
     5281 caggtattgg ttttgcgcta agtatagaac gattattgct tgcacttgaa gaagaaggta
     5341 tcgaattaga tattgaagaa aacttagatt tattcattgt tacaatgggt gatcaagcag
     5401 atcgatatgc tgtgaagtta ttaaatcatt tgagacataa tggtattaaa gcagataaag
     5461 actacttaca gcgtaaaatt aaaggacaaa tgaaacaagc agaccgttta ggtgccaagt
     5521 ttacaatcgt tattggtgat caagaattag aaaataataa aatcgatgtt aaaaatatga
     5581 caactggtga atctgaaaca attgaattag acgcattagt cgaatatttt aagaagtaga
     5641 gagggcgtta aaatatgagt aagagaacaa cttattgtgg attagttact gaagcatttt
     5701 taggacaaga aattacatta aaaggatggg ttaacaatcg tcgtgaccta ggtggattga
     5761 ttttcgttga tttaagagat agagaaggaa ttgtacaagt cgtgtttaat cctgcatttt
     5821 cagaagaggc attgaaaatt gctgaaacag tacgttctga atatgttgta gaagttcaag
     5881 gtacagttac gaagcgtgac cctgaaacag ttaatcctaa aattaaaact ggccaagttg
     5941 aagtacaagt tacaaatatt aaagtgatta ataaatctga gacaccacca ttttctataa
     6001 atgaagaaaa tgttaacgtt gatgaaaata ttcgattaaa ataccgttat ttagatttac
     6061 gtcgtcaaga gttagcgcaa acatttaaaa tgagacatca aattacacgt tctattcgtc
     6121 aatatttgga tgatgaaggg ttctttgaca tcgaaacacc agtactaacg aagtcaacac
     6181 ctgagggtgc acgtgactat ttagtaccat ctcgtgttca tgatggtgaa ttttatgcat
     6241 taccacaatc accacaatta tttaagcaat tattgatgat tagtggattt gacaaatact
     6301 accaaatcgt aaaatgcttc cgtgacgaag atttacgtgc agatcgtcaa cctgaattta
     6361 cacaagtcga tattgaaatg agttttgtag accaagaaga tgtgatgcaa atgggtgaag
     6421 aaatgcttaa aaaagttgtt aaagaagtta aaggcgttga aattaatggc gctttcccac
     6481 gcatgacata taaagaagcg atgcgtcgct atggttctga taaaccagat acgcgttttg
     6541 aaatggaatt aattgacgtt tctcaattag gacgtgatat ggactttaaa gtatttaaag
     6601 atactgttga aaatgatggt gaaattaaag caattgtcgc taaaggtgca gctgaacaat
     6661 atactcgtaa agatatggat gctttaacag aatttgtaaa catctatggt gctaaaggat
     6721 tagcgtgggt taaagttgtg gaagatggtt tgacaggtcc aattggacgt ttctttgaaa
     6781 cagaaaatgt tgaaacatta cttacattaa ctggtgctga agctggtgac ttagtaatgt
     6841 ttgttgcaga caaaccaaat gtcgttgcac aaagtttagg tgcattacgt gtcaaattag
     6901 ctaaagaatt aggtttaatc gatgaaacaa aattaaactt cttatgggtg acagattggc
     6961 cattattgga atatgatgaa gatgcgaaac gttacgttgc agcacatcat ccatttacat
     7021 ctccaaaaga agctgatatt gctaagcttg gcactgcgcc agaagaagct gaggcaaatg
     7081 cttatgacat agtattaaat ggttatgaat taggtggcgg ttcaatcaga attcatgatg
     7141 gtgagttaca agaaaaaatg ttcgaagttc ttggatttac taaagaacaa gcacaagaac
     7201 agttcggctt cttactagat gcatttaaat atggtgcacc accacatggc ggtattgcat
     7261 taggattaga ccgattagtc atgttattga ctaatagaac aaacttgaga gatacaatcg
     7321 cattccctaa aacagcatct gctacatgtt tattaacgaa tgcgcctggt gaagtttctg
     7381 ataaacaatt agaagaactt tctttgcgaa ttcgtcacta aattttaaaa aatcaataaa
     7441 ctttggtttg ttataatttc attaatttta attatgtggt aatataacat cataagatag
     7501 tcatctgagt tgttcgtaag tttgcttttt aatttgggcc taacactctt tgatcaaggg
     7561 agcccaatgg gttttcttgc agcgtacacg ccttatatga aggacgtaca aaacaagaaa
     7621 cagggcaccc acctgtatat aacaggccga atgatcaagc tattataact acggcaatac
     7681 ggactctatc gatacgcaag actttgtctt gcgtattttt atgcgtttct ttaagttttt
     7741 aataaagaat tagttgggtt ttatagcgtt taataaatgt ttcctcgttg aattttggga
     7801 aaataatcaa agcgataatg ctaaatataa aattttaagt tactatgtac ttatgtataa
     7861 atcagtgaaa gaggtaaaat aatgaaacat caattttcaa gaaatgaact agcgattgga
     7921 caacaaggtt tagatttatt gaagcaacaa actgttgtca tacttggcgt tggaggtgtt
     7981 ggttcatttg ctgctgaagc attagcaaga acgaatattg gtcatattat attaatagat
     8041 aaagatgatg ttgatattac aaatgtaaac agacaattac acgctttaac tacaacggta
     8101 ggacaaagta aagtttcttt gatggaagaa cgtattaaat taattaatcc agattgtaaa
     8161 gtcacgccct tacatatgtt ttatacagaa gagacatatg aagaaatttt cgataactac
     8221 gatatagatt attttgtaga tgcaagtgat acaataatgt acaaagtaca cttaatgaaa
     8281 gagtgtcttg accgagggat taaagtgatt tccagtatgg gtgctgcaaa taaaacagat
     8341 ccaacacgtt ttacaatttc agatatctct aaaacacata ccgatccaat ggccaaaatt
     8401 attcgtcaaa aattgaaaaa actaggaatt cgtaaaggga ttccagttgt attttcagat
     8461 gaaagtccaa ttgtcataag agaagatgta aaagatatag ttggagataa aaatgctatc
     8521 aatcgaaaag gacaaatgcc accttcttca aatgcctttg tgccaagtgt tgttggatta
     8581 atttgtgcta gttatgtggt gaatgacgta ttaaaagata ttccagttcg tcgcattaaa
     8641 gacaaagggc aataattcat tttgaaagtc ataattttca acgtaaggca agtgtaacca
     8701 caccataaaa ctaaaactga ctagttcgca agcatagtag aatatgcctc gtatactagt
     8761 cagttttgat ttgattatag ctaaaatatc aaaggtaatc gatttaatct tatttttacg
     8821 gcctttgttt aagtaagttg ttatatattt ctttgagttg ttgttcactt ttagatgtcg
     8881 tttttggttc ataataaatt ttgtttttaa gtttatcagg taaatattgc tgtgaaacat
     8941 agccattaac atattggtgt ggatatttgt aaccaataga tcggcctaga tctttagcac
     9001 cttgataatg tccatctttt aaatggtttg gaatttggcc cacatgaccg tttctaatat
     9061 cggacaatgc actatcaatg gcactcattg ctgaatttga cttaggtgat aagcatagtt
     9121 cgattactgc ttggcttagt ggaattctag cttctggtaa acctagacgt tctgctgatt
     9181 caatagcagc aagtgttctc tgaccagcat taggtgaggc taagcctata tcctcatagc
     9241 taattacaag taatcgtcga actattgtag gtaaatctcc agcttcaatt aatcgtgcta
     9301 aataatgtaa agcggcattg acgtcgctac cacggataga tttttggaaa gcgctcataa
     9361 catcgtaatg catgtcccca tccttatcac ttacaaatgc acctttttgt aaacagtctt
     9421 tagcatcttg caatgtaaca tgtcgataac cgtctttgtc attatctgcg cttaatacag
     9481 ctaattccaa tgcatttaac gcactacgaa catcaccttg actttgtgta gaaaagtagg
     9541 tcatggcatc ttcatcaatt ttgggttgat atgttttcaa accattctct tcatcttcta
     9601 ttgcacgagt taacgcttgg cgcacatctt catcatttaa aggatataac tcaaaaattt
     9661 gcgctcttga acgaatcgct ggattgatag catgataagg atttgaagtt gtagcaccga
     9721 tcaagacgat tttgccattt tctaaatgag gtaataaaaa gtcttgttta gctttatcta
     9781 gtcgatgtat ttcatctaat aacaagataa cttgaccaga cattttagct tcttcaacaa
     9841 caagttgcat atctttttta gtgttagtta cagcattcaa ttgtctgaat ttatattgcg
     9901 tactgcccga aattgctttg gcaatacttg ttttgcctat accaggtgga ccataaaaaa
     9961 tcattgaagt taattttttt gtatcaacca ttcttctgat aatgcctctt ggtccaacta
    10021 aatgttgttg ggaaatgatt tcatctatat tttttgggcg cattctcgat gctaatggtt
    10081 ctgtactcac atttgtcaca cctttcttat actatcttaa ctcaaaaaat gctaaaatgg
    10141 gaatatcaat attcgaatta agtgaggtta tacaatgaaa atttctacta aagggagata
    10201 tggacttaca ttgatgattt ctcttgctaa aaaagagggg caaggatgta tatcattaaa
    10261 gtcaattgct gaagaaaata atttgagtga tttatattta gaacagcttg taggtccttt
    10321 aagaaatgcg gggttaattc gaagtgtacg cggtgctaaa ggtggatacc aattaagagt
    10381 gccagcggaa gaaatctcag caggggatat tataagactg ttggaaggtc caattacatt
    10441 tgttgaaagt attgaatcag aaccacctgc gcaaaaacaa ctatggattc gcatgagaga
    10501 tgcagtgaga gatgttttag ataatacaac attgaaatat ttagcggaat atgtagatac
    10561 aagcgaagat ttagacggat acatgtttta tatttaatcg tataaatact atactataac
    10621 ataaaaactt catattataa tgtttagcga acctccttag tggtatataa atatatacat
    10681 ccaaggaggt ttcactatgg cagacgaaag taagttcgat caatttaaag gaaatgttaa
    10741 agaaacagta ggtaacgtta ctgataacaa agaattagaa aaagaaggtc agcaagataa
    10801 agcgactggt aaagcaaaag aagttgttga aaatgctaaa aacaaaataa ctgatgcaat
    10861 tgataaactt aaaaaataag ttcaattaaa ctaattaaat ataagaggag cgattaatat
    10921 gagttttatg gataaagcta aagacgcagt agaaaaattt aaaaatagcg ataatgaaca
    10981 agttaaaaat gtaaaagata agataaatga atatacagga tcgaataacg aagagaaaaa
    11041 agaaaatgaa gataaagaga aataaatttc tttaaatttt ggatatgctt ttcaatattt
    11101 tgaaagcgcc ccatcaaata aacactaaca cagtgtaaag ttgaaggggc gctttcatat
    11161 ttgctttaga attttaagtg aatttgaatc attactaaaa taaatgattg aacaataaag
    11221 acatctttta ttcaaaatgc t
//
LOCUS       DAMZKC010000087        11026 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.088, whole genome shotgun sequence.
ACCESSION   DAMZKC010000087 DAMZKC010000000
VERSION     DAMZKC010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11026
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.088"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            73..315
                     /locus_tag="POB55_001663"
     CDS             73..315
                     /locus_tag="POB55_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001790022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500625.1"
                     /translation="MNRVIFVYIALIITLVRFFTPIHPSFSNLIYWIFVLYFIPIILC
                     VIGFKAEKLIATMVIIPNFLGILYRLYAYVTHILFM"
     gene            complement(544..906)
                     /locus_tag="POB55_001664"
     CDS             complement(544..906)
                     /locus_tag="POB55_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2294 domain-containing protein"
                     /protein_id="HDI6500626.1"
                     /translation="MKRTKGEIEAEISKAITQWEKDFLGRGSLSVKSDILRDMVIISL
                     QGILTPAEYRVCSTNEGLLNIKRTRSELVESGEQDLNDIIFKITGIKVMSFHSDLSTV
                     TGERIIVFKLEDNLEKHI"
     gene            complement(1068..3773)
                     /locus_tag="POB55_001665"
     CDS             complement(1068..3773)
                     /locus_tag="POB55_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbcC family protein"
                     /protein_id="HDI6500627.1"
                     /translation="MTTQLNINSVIENAKRVITPLSPISIFAARNPWEGLEADTFEDV
                     AKWLRDVRDVDIFPNKALIESAVARGELDESVFNQLVTDMLLEHHYNIPQHYINLYID
                     NIKTLKDVPASYMNHSNVDVVADLLLEKSKRDMADSYHHYDVRPMSDAIIDEQGEPLS
                     EQVNRQMIKWTKLYIDQFLSSWTMPKREQSFYHAWLHLAQHDHSFTKAQRQVIKGLPN
                     DPKMTIESVLTHFSIDQEDYQAYVEGHLLALPGWAGMLYYRSQQHHFEQHLLTDYLAI
                     RLVVEQLLVGDEFKSVTKDCESRSENWFKQTVASWCYYSDMPSDVLLQHDVNEIQTFI
                     HFAATMNKNVFKNLWLIAWEMTYESQLKQKIKAGHESVAGALDVNQVNVSENDNANQP
                     HSVLLNDTQAVDENNSELNQVGTSTKAQIAFCIDVRSEPFRRHIEAAGPFETIGIAGF
                     FGLPIQKDAVDEQFKHDSLPVMVPPAYRIKEFADRYDMNVYRQQQQTMSSMFYTFKLM
                     KNNVMPSLLLPELSGPFLSLSTIVNSIMPRKSRASLQKITQKWLKKPETKLTIDREFD
                     RTSDLPVGFTEQEQIDFALQALKLMDLTEAFAPFVVLAGHASHSHNNPHHASLECGAC
                     GGASSGFNAKLLAMICNRPNVRQGLKQSGVYIPETTVFAAAEHHTSTDTLAWVYVPDT
                     LSSIALDAYESLNDAMPMISEHANRERLDKLPTIGRVNHPVEEAQRFASDWSEVRPEW
                     GLAKNASFIIGRRQLTKGIDLEGRTFLHNYDWRKDKDGKLLNTIISGPALVAQWINLQ
                     YYASTVAPHFYGSGNKATQTVTSGVGVMQGNASDLMYGLSWQSVMAADRTMYHSPIRL
                     LVVVQAPDYVVARLLANNDHFARKVSNHWLRLMSVNEEGRFKSWI"
     gene            complement(3786..5270)
                     /locus_tag="POB55_001666"
     CDS             complement(3786..5270)
                     /locus_tag="POB55_001666"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase subunit 5"
                     /protein_id="HDI6500628.1"
                     /translation="MLSFQLLFSLFVIALIIALISGLLFLAPVMPMRYIKLHLYILVM
                     PVLFAVIGFFGIHGQHVLGPFKIDRLSWLLAGFVMALGFIIQKFSMRYLLGDHHYRHY
                     FPLFTAITSFASLAWMSEDLRLMALCWGMTLLCLTLLMNVNRFWKVPRESAKLSSMTF
                     LCGWLAFVGAIVTIYIATGEWRVPQHMSNSTWSLLTNVLLVLAVMIPAAQFPFHRWLI
                     ESVTAPTPVSAIMHAGIVNAGGVILTRFAPIFDNGFALSLLLILSSISVLLGSGISLV
                     QVDYKRQLVGSTMSQMGFMLVQCALGVYSAAIIHLILHGIFKATLFLQSGSIVKRFNI
                     PKQASAKDAYGWIVMGRVLAIIVAFLFWMSSDRSAYEVLSALILAWSLLVSWNQMVAF
                     SKGRMARLVGMILIAIVTFIYVITHNYFYDVLQNITTHATTPPTVSVIISVVILIFGS
                     LLSIWVARHRYSKGFAVLYVWLVNLGEARSKAIESHPNYLKKYL"
     gene            5790..5855
                     /locus_tag="POB55_001667"
     CDS             5790..5855
                     /locus_tag="POB55_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-1"
                     /protein_id="HDI6500629.1"
                     /translation="MGIIAGIIKVIKSLIEQFTGK"
     gene            5887..5952
                     /locus_tag="POB55_001668"
     CDS             5887..5952
                     /locus_tag="POB55_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-2"
                     /protein_id="HDI6500630.1"
                     /translation="MGIIAGIIKFIKGLIEKFTGK"
     gene            6004..6072
                     /locus_tag="POB55_001669"
     CDS             6004..6072
                     /locus_tag="POB55_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-3"
                     /protein_id="HDI6500631.1"
                     /translation="MEFVAKLFKFFKDLLGKFLGNN"
     gene            6136..6198
                     /locus_tag="POB55_001670"
     CDS             6136..6198
                     /locus_tag="POB55_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-4"
                     /protein_id="HDI6500632.1"
                     /translation="MAIVGTIIKIIKAIIDIFAK"
     gene            complement(6855..8057)
                     /locus_tag="POB55_001671"
     CDS             complement(6855..8057)
                     /locus_tag="POB55_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDI6500633.1"
                     /translation="MAKIPVTVLSGYLGSGKTTLLNHILQNREGRRIAVIVNDMSEVN
                     IDKDLVADGGGLSRTDEKLVELSNGCICCTLRDDLLKEVERLVKKGGIDQIVIESTGI
                     SEPVPVAQTFSYIDDELGIDLTAICRLDTMVTVVDANRFVHDINSEDLLMDRDQSVDE
                     TDERSIADLLIDQVEFCDVLIINKIDLISEEELAKLEKVLSALQPTAKIIKTINSEVD
                     LKEVLNTQRFDFEKASESAGWIKELESGGHASHTPETEEYGISSFVYKRRLPFHAKRF
                     NDWLETMPNNVVRSKGIVWLAQYNHVACLLSQAGSSCNIHPVTYWVASMSEAQQTQIL
                     AERQDVAAEWDPEYGDRHTQFVIIGTDLDEEKLTKELDACLVNAQEIDADWQQFEDPY
                     QWQIRPAR"
     gene            8189..8449
                     /locus_tag="POB55_001672"
     CDS             8189..8449
                     /locus_tag="POB55_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500634.1"
                     /translation="MSTLLYNGDKDISHERESRCINMLFVILVLYIIGITFILLSVFG
                     SKTEGLSTKHTLYTIGSAIITIAIFISIGYAIQYLTAALYGL"
     gene            complement(8733..9047)
                     /locus_tag="POB55_001673"
     CDS             complement(8733..9047)
                     /locus_tag="POB55_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500635.1"
                     /translation="MTLIEPDMTLRMPDISTTVETLNLISKMNAQKENIRSVVAPEHK
                     HKYKDIENGLKGEEKVLIEQMAHHCEAFKANFKGAAQGDWVKSAMSEIDSIKDDLKKI
                     NS"
     gene            complement(9066..9389)
                     /locus_tag="POB55_001674"
     CDS             complement(9066..9389)
                     /locus_tag="POB55_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500636.1"
                     /translation="MMFNQINNKNELEESYESEKKRIENELQNLNELRHRTRKENERS
                     YDVFQYLKHEMNYSEDAQRKMTRNIEAYEQEINEIIRKQEWKLEEYKEDLKKSYEKQL
                     DKLSD"
     gene            complement(9386..10711)
                     /locus_tag="POB55_001675"
     CDS             complement(9386..10711)
                     /locus_tag="POB55_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001581953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500637.1"
                     /translation="MKDKYKIDPGPIKYNTEETTAISKISYEIENAKLYGIKSKTIGR
                     QIDQLKEAKKFPSNLEYVDSYTDSLNGVTTSAFLNKDTGKVTLGMTGTNIQGEAFKKL
                     KEGEFSRQNFTNALETVKDGYADLKILYSPASDQNYRYANTQEFINKIKSKYDIDFIT
                     GHSLGGRDAVVLGMSNGIPNIVVYNPAPISITSLNPNSPDGKRLLELYKNYKGNITRF
                     VAENDALTENLKKYKHYVFFGNDKVFKNGKGHEMEGFLTEEEQKAIKKELKKLQGYAE
                     ENNKSFVKHSNNAISKLASIELLRANMMTTNGGGLSSSQQKVLESLTALTIAQSFSQL
                     IDDEINQIKKMYNEKKKKFGKNWEDAQKAGKAVGEDLSVNGVLNALDEGQVNESSMVR
                     EPEQMISAKERQLSTIGSSVSNYIMRVRLSINEIVDKDQVLASQIGGLL"
     gene            complement(10701..>11026)
                     /gene="lpl8"
                     /locus_tag="POB55_001676"
     CDS             complement(10701..>11026)
                     /gene="lpl8"
                     /locus_tag="POB55_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl8"
                     /protein_id="HDI6500638.1"
                     /translation="KFFGQYANLKELKNYNNGDVSINENVPSYDAKFKMSNKDENVKQ
                     LRSRYNIPTDKAPVLKMHIDGNLKGSSVGYKKLEIDFSKGEKSDLSVIDSLNFQPAKV
                     DEDER"
BASE COUNT         3403 a         2150 c         1662 g         3811 t
ORIGIN      
        1 attgaaataa caatattttg ctattttcaa tttagtacga tctatattta ttatacagag
       61 ggggtaaggc gtatcaatag agttattttt gtctatatag cgttaatcat tacattagtt
      121 agattcttta cccctattca tccatcgttt tcaaatttga tttactggat atttgtatta
      181 tattttattc ctattatact atgcgttatc ggtttcaagg ccgaaaaact tattgcaaca
      241 atggtcatta tacctaattt tttaggaata ctttatcgat tatatgccta cgtcacacat
      301 atactcttta tgtaaaagga tagtgggcat gtctcgcaac aaatatgctt gcgtcgactt
      361 gtcaccattc gcaaatttta tgacatacgc ctttcacggg ctatattcag acccacgcat
      421 tcatccacgt aataaacaca tcatgtaata gaaaaacagc acacccaaat atatggcgtt
      481 gcgctgttta accaagcata cttctatagc tttaataagc cagcaaaagc atacctaacc
      541 ttcttaaata tgcttttcca aattatcctc aagtttgaat acgataatac gttcacctgt
      601 aactgtactt aaatcactat ggaagctcat cactttgata cctgtaattt taaaaatgat
      661 atcattcaaa tcttgctcac cggattcaac taattcagaa cgtgttcgtt taatatttag
      721 taatccttca ttcgtactac acacacgata ttctgctggc gttaagatac cttgtaaact
      781 aataatcacc atatctctta aaatgtctga tttaactgac aaggaacctc tgccaaggaa
      841 atctttttcc cattgcgtaa tggctttact gatttcagct tcgatttcac ctttcgttct
      901 tttcatatca ctaactccaa taatattaaa attgattact tcatctttgt atcgttatca
      961 cgacatcaac tattacatta agtttatcat ttttagtata ttttaagaag ctagaacatt
     1021 gtagatatga tgatatatta gttacttagc atcgcaacat atcatcgtta aatccaactt
     1081 ttaaaacgcc cttcctcatt aacgctcatt aaacgcagcc aatgattaga caccttccta
     1141 gcgaaatggt cattattcgc gagtagtctt gctacaacat agtcgggtgc ctgaacaacg
     1201 acaagcaaac gaattggcga atgatacatc gttcgatcag cagccataac agattgccat
     1261 gataagccat acatcagatc actcgcatta ccttgcatga caccaacacc tgacgtgacg
     1321 gtttgtgtcg ctttattccc acttccgtaa aaatgcggcg caactgtcga cgcataatat
     1381 tgtaaattaa tccattgtgc cacaagcgcc ggaccagaaa tgatagtatt taacaatttg
     1441 ccatctttat ctttacgcca atcataattg tgtaaaaatg tccgcccttc taaatcaatg
     1501 ccttttgtta attggcgtcg tccaattata aatgatgcat ttttagccaa gccccattct
     1561 ggacgtacct cactccaatc actcgcaaac cgctgcgctt cttccactgg atgattcaca
     1621 cgaccaatcg ttggcagttt gtccaaacgt tcgcgattcg cgtgttcaga aatcatcggc
     1681 atcgcgtcat tcaatgattc atatgcatct aaagcaatag aagataatgt gtctggcaca
     1741 tatacccatg ccaacgtatc agtagacgta tgatgttctg ctgccgcaaa aacagttgtc
     1801 tctggaatat acacacctga ttgttttaat ccttgtctga catttggacg attacatatc
     1861 atcgctaata acttagcatt aaaaccgctt gatgcgccac cacaagcccc acattcaagt
     1921 gatgcatgat gtggattatt gtgagaatga ctagcatgac ctgctaacac aacgaacggc
     1981 gcaaatgctt cggttaaatc catcaatttc aacgcttgta acgcgaaatc aatttgctct
     2041 tgctcagtaa atccaacagg taagtctgat gttcggtcaa actcacgatc aatcgttaac
     2101 tttgtttcag gctttttcaa ccatttttgt gttatttttt gtaaagacgc gcgacttttt
     2161 ctaggcataa tcgaattgac aatggtactc aagcttaaaa atggcccact taattcaggc
     2221 aataacagac taggcataac attatttttc atcaatttaa atgtgtaaaa catcgatgac
     2281 attgtctgtt gctgttgtcg ataaacattc atatcgtagc ggtctgcaaa ttctttaatg
     2341 cgatatgccg gcggtaccat gacaggtaat gaatcatgtt tgaattgttc gtctacggca
     2401 tctttttgaa taggtaatcc aaagaaacct gcaataccaa tcgtttcaaa gggccctgct
     2461 gcttcgatat gtctacgaaa tggttctgaa cgaacatcta tacaaaatgc aatttgcgct
     2521 ttcgttgatg tgcccacctg atttagctcg ctattatttt catcaactgc ttgtgtgtca
     2581 tttaataata ctgaatgtgg ctgattagcg ttatcatttt ctgagacatt tacttggttt
     2641 acatctaatg cgcccgccac actttcatga cctgctttaa ttttttgttt taactgagat
     2701 tcgtatgtca tttcccaggc aattagccat aaatttttaa atacattttt attcatagtt
     2761 gctgcaaaat gaataaacgt ttgaatttca ttgacgtcat gttgtagtaa tacatcgcta
     2821 ggcatatcac tgtagtaaca ccatgatgca acagtttgct taaaccaatt ttccgatcta
     2881 ctttcacaat ctttagtgac tgacttaaac tcatcaccaa ctagcaattg ttcgacaact
     2941 aaccgaattg ccaaataatc cgttaacaaa tgttgttcaa agtgatgctg ttgtgaacgg
     3001 taatacaaca tacctgccca acccggtaac gccaaaagat gtccttcaac ataagcttgg
     3061 tagtcttcct gatctattga aaaatgagtt aatactgact ctatcgtcat tttaggatca
     3121 ttgggcaagc ctttaatcac ttggcgctgt gctttagtaa aactatgatc atgttgcgct
     3181 aaatgcaacc atgcatggta aaaactttgc tcacgcttcg gcattgtcca actcgataga
     3241 aattgatcga tataaagttt cgtccattta atcatttgac gattcacttg ttcgctaagt
     3301 ggctcacctt gttcatctat tattgcatca ctcatcggac gtacatcata gtgatgatat
     3361 gaatcagcca tatcacgttt tgatttttct aatagtagat cagcaacaac atcaacattt
     3421 gaatgattca tatatgatgc aggtacgtct tttaatgttt taatgttatc aatataaaga
     3481 ttgatgtagt gttgcgggat attgtagtga tgttcaagta acatatcagt aacaagttga
     3541 ttaaagacac tttcgtctaa ttcaccacgt gccacagcac tttctattaa tgctttattt
     3601 ggaaaaatat ccacatctcg aacatcacgt aaccattttg caacatcttc aaacgtatcc
     3661 gcttctaatc cttcccatgg atttcgtgct gcaaaaatcg aaatcggtga taatggtgta
     3721 ataacacgtt tcgcattttc aatgactgaa ttgatattta actgtgttgt catacctttc
     3781 acctcctata aatacttctt caaataattc ggatgacttt ctatcgcttt cgagcgtgct
     3841 tcacctagat taactaacca cacgtacaat accgcaaaac ccttagagta tcgatgacgc
     3901 gccacccaaa tacttaataa actaccaaat attaaaatga caacactaat gatgacactc
     3961 actgtaggcg gcgttgtcgc atgtgttgtt atattttgta atacatcgta aaaataattg
     4021 tgtgtgatga cgtagataaa tgtcacaatt gcaatcaaaa tcatgccaac cagacgtgcc
     4081 atgcgccctt tactaaaggc taccatctga ttccaagata caagtaatga ccatgctaga
     4141 atgagtgcac ttaacacttc atatgcactt ctgtcactac tcatccaaaa tagaaatgcc
     4201 acgataatag ctaatacacg tcccatgaca atccagccat aagcgtcttt agcagatgct
     4261 tgttttggaa tattgaatcg cttcacgata gaacctgatt gtaaaaataa tgttgcttta
     4321 aaaataccgt gcaatattaa atgaataatc gctgctgaat atacacccaa tgcacattga
     4381 actaacataa agcccatttg actcatcgta gagccgacta actgtctttt gtaatccact
     4441 tgaaccaagc taatacccga tcctaacaat acagaaatac tagaaaggat aagtaataat
     4501 gataacgcaa atccattatc aaatatcggc gcaaaacgag ttagaataac accacctgca
     4561 ttcacaattc ctgcatgcat aattgccgat actggcgttg gtgctgttac agattcaatc
     4621 aaccatcgat gaaatggaaa ttgtgctgct ggtatcatga cagctaatac aagtagtaca
     4681 ttcgtcaaca atgaccatgt cgaattagac atatgttgag gcacgcgcca ctcgccagtc
     4741 gcaatataaa tagttacaat tgctccaacg aatgcaagcc aaccacataa aaatgtcata
     4801 cttgataatt tcgcagactc acgtggcact ttccaaaaac gattaacgtt catcagcaat
     4861 gttaaacata ataatgtcat accccagcag agtgccatca gtcttaagtc ttcagacatc
     4921 catgctaaag atgcaaacga cgtaatcgca gtgaacaatg gaaagtaatg tctataatga
     4981 tgatcaccta gtaaatatcg cattgaaaac ttttgaataa taaaaccaag cgccattaca
     5041 aagccagcta ataaccaaga taaacgatct attttaaatg gacctaagac atgttgacca
     5101 tgaataccga aaaagccaat gactgcaaat aatactggca tgactagtat gtataaatgt
     5161 aatttaatat atctcattgg cataactggt gctaaaaaca acaagccact tatcaatgca
     5221 atgataagcg caataacaaa cagtgaaaat agcaattgaa aacttaacac tgcataacct
     5281 ccttatttct aatctctcgc ataattgctt atgtataaaa taaaaaccta caatagtaga
     5341 ttctgtacat aatggcagaa aatttactat tgcaggtttc agtttaacta gacactgcat
     5401 cacggtacgt tgatatacct tgttgcattg ttctctttaa gcgtgctccc atgcacatat
     5461 gtatataaaa tgttacttct gtctgttcaa ttcatcttca taaatatgct ttgcctagac
     5521 gagacctaac gtgttattcg ttttaaactt ataacataaa atataattaa atttctgctt
     5581 catgtcaaat tcatgagctt aacctctatt aaaccaatga ttgtaaagat tttgtaaatg
     5641 cacctgtaca gttaggcagt atttcccgtc cttttaaaat aaaaaattcg cagttatgat
     5701 cataacaatt caagttagga aaaaaatcaa ttacgcacaa gataactatg tacaatgaag
     5761 ttaactcata agcaaaggag gtaatcttaa tgggtatcat cgctggcatc attaaagtta
     5821 tcaaaagctt aatcgaacaa ttcactggta aataagattt cataacaaac aaaggaggtc
     5881 tttcacatgg gtatcattgc aggaatcatt aaattcatta aaggattaat tgagaaattc
     5941 actggtaagt aagttataaa aatctcatag atatgaacat cttatttgaa gggggccatt
     6001 cacatggaat tcgtagcaaa attattcaaa ttctttaaag atttacttgg taaattttta
     6061 ggtaacaact aatctcaaac attaacgatc aacaactcat cactatgtta aatcaacata
     6121 caggaggaca aaacgatggc tattgtaggt actatcatta aaatcatcaa agcaattatc
     6181 gacattttcg caaaataatt taagcgaatt gaatacttaa aattctcagg ccactatacc
     6241 aatagggatg gtggctttgc tttgtgttga tttattgatg tgaggtgagt cttgttagtt
     6301 tgttgcaaat aaatggtctt ggttttttgt ataggatgtt cttggtggga cgtacggaat
     6361 attcgtgatc tttgtagtct gacgcgttat atttttgtgg cgtgttttat gtttgatact
     6421 cgaggtctga gacattcatg atttggcata cgaaatctta atgattttca taatctatcc
     6481 caagatatat tggccacgtg cagaattgcg ttgcacgttt agactgaaac actcgtgtga
     6541 ccgtaagtgt taatagtaca ttgatagctg catttacttc actcattttt atgactgtta
     6601 aacaatgatt gtaccttcaa ttaacagttg gtacgatggt tttgccattt ttcatcaaag
     6661 caaacataaa aaggactaag acatatacat gtcctagccc tatggataaa atgcaaattt
     6721 ctgctttatc aaaactatca cactttagat agattgaaaa caaaaagatc ctaagaacat
     6781 cttaactttt tattaattgt cataaattgc aaacaattaa gccacaattt aaaaatgatt
     6841 atacttcatt caacttatcg tgctggtcta atttgccatt gatatggatc ttcaaattgt
     6901 tgccaatctg catcaatttc ttgcgcattg actaagcatg catcgagttc ttttgttaat
     6961 ttttcttcat ctaagtcggt accaataatg acaaattgtg tatgacgatc gccatattct
     7021 ggatcccatt cagctgcgac gtcttggcgt tctgctaata tttgcgtttg ttgtgcttca
     7081 gacatactag ccacccaata tgtaactgga tgaatattgc aagatgaccc tgcttgagat
     7141 aataaacatg ctacgtgatt gtattgtgct agccatacga taccttttga tcgaacgaca
     7201 ttatttggca tggtttctaa ccaatcattg aaccttttag catggaaagg aagtcgacgt
     7261 ttatatacaa acgatgatat accatattct tctgtttcag gtgtatgcga tgcatgccca
     7321 ccagactcaa gttctttgat ccatcctgct gactcgctcg ctttttcaaa atcaaaacgc
     7381 tgcgtattta agacttcttt taaatccact tcagaattta ttgtcttaat aattttagca
     7441 gtaggttgca atgcacttaa cactttttct aacttcgcaa gttcttcttc actaattaaa
     7501 tcaattttat taataatcaa tacatcacaa aattcaactt ggtcaattaa taaatcagca
     7561 atcgaacgct catctgtttc atcaacgctt tgatcacgat ccatcaataa atcttctgag
     7621 ttgatgtcat gtacgaagcg gttagcatcc acaactgtaa ccattgtatc taaacggcaa
     7681 atcgctgtaa gatcaatgcc aagttcatca tcaatatatg agaaagtttg tgcaacaggt
     7741 actggctctg aaatccctgt tgactcaata acaatttgat cgatgccacc ttttttcact
     7801 aaacgctcaa cttcttttaa taaatcgtct ctaagtgtac aacagataca accattagaa
     7861 agttcgacta atttttcgtc tgtgcgcgat agtcccccac catctgcgac aagatcttta
     7921 tcgatattga cttcactcat atcatttaca attaccgcaa tacgtcgacc ttctcgattt
     7981 tgtaaaatat gatttaacaa cgttgttttc cccgagccta aataaccact taataccgta
     8041 actggaattt tagccatata tataccctca cttcaattta tttgtttcgt tcgtttgcaa
     8101 ataggaataa ttccgtttta cattatatag gagcatttcc tctttcgcaa tcttcgataa
     8161 taaaaaaata gtatacttaa ttaaattatt gagcacttta ctttataatg gagacaaaga
     8221 tatatctcac gagagagaat cgaggtgtat aaacatgcta tttgtcattt tagttttata
     8281 tatcattggt attacattta ttctactcag tgtttttggt tcaaagactg aaggattatc
     8341 tacgaaacat actttatata ccattggcag tgctattata acgattgcta ttttcatttc
     8401 aattggctat gccattcaat acttaactgc agcgctttat ggtttgtaag gtgaggatga
     8461 tttatggcgg atagttcgag agagtttgac ttgcgttgat tttgataaag aggtcatagc
     8521 ttttggtact tgaggatttt gttgttgctg tttggaattt ggatatgttt aatgcgggac
     8581 agtaatttaa gttgtgtttt tacaattgta agtgtgatat ttcgattcag ttcgaattac
     8641 ttcacatggg aataaaataa aaagaagcgg cctagtgtca gttgtgaata cactgaacat
     8701 tggtcgcttt atttagtagt acgatatgta gtttagctat taattttttt caggtcatcc
     8761 ttaatgctgt ctatctcaga catggcactt ttaacccaat ctccttgagc cgcgccttta
     8821 aaattagctt taaaagcctc gcaatgatgc gccatttgtt caattaatac tttttcttca
     8881 ccttttaatc cattttcaat atctttatat ttatgcttat gttcaggtgc aacaactgaa
     8941 cgaatgtttt ctttttgagc attcatttta gatatgagat taagtgtttc tactgtagta
     9001 cttatatctg gcattcttaa ggtcatatct ggttctatta gagtcattta atctcctcca
     9061 aattatcagt cacttagttt atctaactgc ttttcataag acttttttaa gtcttcttta
     9121 tattcttcta atttccattc ttgctttcta attatctcat tgatttcttg ctcatacgct
     9181 tctatatttc tcgtcatttt cctttgtgca tcttcactat aattcatttc gtgtttcaaa
     9241 tattgaaaaa catcataact acgttcattt tcttttcgag ttctatgcct aagttcattt
     9301 aaattttgca gttcattctc tatacgtttt ttctcagatt catatgattc ttctaattca
     9361 tttttattat taatttgatt aaacatcata gtaatccacc tatttgtgat gcaagcactt
     9421 gatctttatc aacgatttca ttaatactga gtctaactct cataatataa tttgatacag
     9481 aagaccctat cgttgaaagt tgtctttctt ttgcagatat catttgttca ggttctctta
     9541 ccatactact ttcattcact tgaccttcat ctaaagcatt gagaactcca tttacactca
     9601 aatcttcacc tacagctttt ccagcttttt gagcgtcttc ccaatttttt ccaaatttct
     9661 ttttcttttc attatacatt tttttgattt gattaatttc atcgtctatc agttgactga
     9721 atgactgcgc aattgttaaa gctgttaaac tttctaaaac tttttgctgc gaagaagata
     9781 atcctcctcc atttgtagtc atcatatttg ctctaagtaa ttctatacta gctaatttag
     9841 agatagcatt atttgaatgc tttacaaatg acttattatt ttcttctgca taaccttgta
     9901 gttttttaag ttcttttttt atagcttttt gttcttcttc ggtcagaaag ccttccattt
     9961 catgaccttt accattttta aagactttat cattaccgaa aaaaacataa tgcttatatt
    10021 tcttcagatt ttctgtcaat gcatcatttt ctgcaacaaa cctagtaata ttacctttat
    10081 aatttttata taactctaac aatcgttttc catctgggga attaggattc aaactagtta
    10141 tagaaatagg agctggatta taaaccacaa tgttcggaat accattactc attcctagaa
    10201 ctaccgcatc tcttccacct agtgaatgtc cagtaataaa atcaatatca tacttacttt
    10261 ttattttatt tataaattct tgcgtattcg catatctata gttttgatca gatgcaggag
    10321 aatataatat tttaagatct gcatatccat ctttaactgt ttccaaagca ttggtaaaat
    10381 tttgtcttga aaattcacct tcttttaact ttttaaaggc ttcgccttgt atattagtcc
    10441 cagtcatccc aagagttact ttgcctgtat ctttatttaa aaaagcagaa gttgttactc
    10501 cattaagaga atcagtataa ctatcaacat attcaagatt agaagggaat tttttcgctt
    10561 cttttaattg atctatttgt ctacctattg ttttactttt aattccatat aatttcgcat
    10621 tttcaatttc ataacttatt ttagaaatag ctgtagtttc ctcagtatta tatttaatag
    10681 gtcctggatc tattttgtat ttatctttca tcttcatcca ccttcgccgg ctggaaattt
    10741 aatgaatcta ttactgacaa atcgcttttt tcaccttttg aaaagtcaat ttccaacttt
    10801 ttataaccca cagaacttcc tttcaaatta ccatcaatat gcatttttaa taccggtgct
    10861 ttatcagtag gaatattata acgacttctt aattgcttaa cattttcatc tttattgctc
    10921 attttaaatt ttgcgtcata acttggaaca ttctcattaa ttgagacatc accattgttg
    10981 taatttttca attctttaag gtttgcatat tgtccgaaaa atttaa
//
LOCUS       DAMZKC010000088        10935 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.089, whole genome shotgun sequence.
ACCESSION   DAMZKC010000088 DAMZKC010000000
VERSION     DAMZKC010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10935
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.089"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            87..695
                     /locus_tag="POB55_001677"
     CDS             87..695
                     /locus_tag="POB55_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HDI6500639.1"
                     /translation="MDFVNNDTRQIAKNLLGVKVIYQDTTQTYTGYIVETEAYLGLND
                     RAAHGYGGKITPKVTSLYKRGGTIYAHVMHTHLLINFVTKSEGIPEGVLIRAIEPEEG
                     LSAMFRNRGKKGYEVTNGPGKWTKAFNIPRAIDGATLNDCRLSIDTKNRKYPKDIIAS
                     PRIGIPNKGDWTHKSLRYTVKGNPFVSRMRKSDCMFPEDTWK"
     gene            1032..2240
                     /gene="gltS"
                     /locus_tag="POB55_001678"
     CDS             1032..2240
                     /gene="gltS"
                     /locus_tag="POB55_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HDI6500640.1"
                     /translation="MIELNAITTLCLACILYLLGKAIVNHVNFLKRICIPAPVIGGLI
                     FAILVAALDSFGMVKIKLDASFIQDFFMLAFFTTIGLGASLKLFKLGGKLLLLYFMFC
                     AIISVIQNIVGVSLAKVLNIKPLLGLTAGSMSMEGGHGNAAAYGKTIQDLGIDSALTA
                     ALAAATLGLVFGGLIGGPVVKFLIKRYNLKPQHSDDTFKDYSQVAYNEHLHSKFNATE
                     VFFIQFTIVVFCMAVGSYFSHLFTSQTGINVPIYVGSLFVAVIVRNISESFNFNIVDL
                     KITNQIGDVALGIFLSLALMSIQLIEIYKLAIPLIIIVLVQVVVMILFAVLILFRGLG
                     KDYDAAVMVGGFIGHGLGATPNAMANLDVITKKYGNSPKAYLVVPIVGAFLIDLIGVI
                     VIMGFIQWFS"
     gene            complement(2288..3337)
                     /locus_tag="POB55_001679"
     CDS             complement(2288..3337)
                     /locus_tag="POB55_001679"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HDI6500641.1"
                     /translation="MSDFQREQRKNEHVEIAMAQSDAMYSDFDKMRFVHHSIPSINVN
                     DIDLTSQTPDLTMAYPVYINAMTGGSEWTKNINEKLAVVARETGLAMAVGSTHAALRN
                     PRMAETFTIARKMNPEGMIFSNVGADVPVEKALEAVELLEAQALQIHVNSPQELVMPE
                     GNREFVTWLDNIASIVSRVSVPVIIKEVGFGMSKELMHDLQQIGVKYVDVSGKGGTNF
                     VDIENERRANKDMDYLSSWGQSTVESLLETTAYQSEISVFASGGLRTPLDAIKSLALG
                     AKATGMSRPFLNQVENNGIAHTVAYVESFIEHMKSIMTMLDAKNIDDLTQKQIVFSPE
                     ILSWIEQRSLNIHRG"
     gene            complement(3368..4315)
                     /gene="corA"
                     /locus_tag="POB55_001680"
     CDS             complement(3368..4315)
                     /gene="corA"
                     /locus_tag="POB55_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HDI6500642.1"
                     /translation="MALKIRYQTTYEPFKVVDDIKEIPKDATIVWYDFDEPNEQENEW
                     FKAHFNFNDLEVDDAINGMPRAKYKSYKDYQYLVFHSIMGSNYSPIALNIFIQDNVLV
                     TYHHQTLESLNKVVYKYMNTLDAELDCADVVILILDMMVDKYFNFVYALEDSVYHFED
                     RHVDDRFNKMVMDSVFKLRSDLIKVKRVLFPMQELIDTMKQNGDLIIDNKHSLYIQHI
                     DDHLIKQRNIIRTAQEMTNEIRENFESYTSFRMNSIMQVLTLVSVIFSPLTFIAGIYG
                     MNFVNMPALHLHYGYYICLAVMFVIAVVLIIFFRRKKWF"
     gene            4412..4903
                     /locus_tag="POB55_001681"
     CDS             4412..4903
                     /locus_tag="POB55_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HDI6500643.1"
                     /translation="MVIIKQLFTHTQTVTSEFIDHNNHMHDANYNIIFSDVVNRFNYS
                     HGLSLKERENLAYTLFTLEEHTTYLLELSLGDVFTVTLYIYDYDYKRLHLFLTLTKED
                     GTLASTNEVMMMGINQHTRRSDAFPESFSTQIAHYYKNQPTITWPEQLGHKIAIPHKG
                     ALK"
     gene            4900..5319
                     /locus_tag="POB55_001682"
     CDS             4900..5319
                     /locus_tag="POB55_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500644.1"
                     /translation="MTDALQQKIHIELLDLLDDVKFELTELNAQKGLYINGPANQLLK
                     RGVHMAYVQGQKQAIDNIMTIVEQQLEDQHFLEHYDKFQNEVAHRNYDKTANFAELSD
                     IPRQFDNFLDQFYQIKGQYFIITHINTLIGDFHSEAH"
     gene            complement(5427..6347)
                     /locus_tag="POB55_001683"
     CDS             complement(5427..6347)
                     /locus_tag="POB55_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDI6500645.1"
                     /translation="MIRNRVMNSVVNKYLLHNRSIMFKNDQDVERFFYKREIENRKKH
                     KQPSTLNVKANLEKLSLDDMQVFRFNFRHQIDKKILYIHGGFNALQPSPFHWRLLDKI
                     TLSTLYEVVLPIYPKTPEFHIDDTFQAIQRVYDQLVSEVGPQNVVVMGDGSGGALALS
                     FVQSLLDNQQPLPNKLYLISPILDATLSNKDISDALIEQDAVLSQFGVNEIMKKWANG
                     LPLTDKRISPINGTIEGLPPVYMFGGGREMTHPDMKLFEQMMLQHHQYIEFYDYPKMV
                     HDFPIYPIRQSHKAIKQIAKSIDDDVTQNN"
     gene            6555..7193
                     /locus_tag="POB55_001684"
     CDS             6555..7193
                     /locus_tag="POB55_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500646.1"
                     /translation="MTNPNQRLEPFDETFQQSNIHRGKRYGKKKRSLVSMIIQIIVVI
                     LTAIAGYSMWKQPIFNIVFTKESVDFDGIKNFNETIGKMHQLNINLGNINDLQQGIDR
                     LILVFNGFFILCIVSLVITILTIVFNRTVLKVVNILVLTIMLVITLYFSYIIKALAQE
                     IADALKKYYLNVPPDQVITEADAIHNGIILLGCSIGLLVISLFFRNRMPRLK"
     gene            complement(7439..9370)
                     /locus_tag="POB55_001685"
     CDS             complement(7439..9370)
                     /locus_tag="POB55_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HDI6500647.1"
                     /translation="MTTTFIISYIILALIIVGVINLFLIRSRKKGKRQQKEQQFTTRQ
                     SNQSKFKASDLDKTTDQSTQRMTHEELRVDNQDDHSQVSLNGYTKGSEKDQEAFTNNN
                     GEEAVAAKNPESEEYKVNEKIKKEHKNFIFGEGVSRGKILAALLFGMFIAILNQTLLN
                     VALPKINTEFNISASTGQWLMTGFMLVNGILIPITAYLFNKYSYRKLFLVALVLFTIG
                     SLICAISMNFPIMMVGRVLQAIGAGVLMPLGSIVIITIYPPEKRGAAMGTMGIAMILA
                     PAIGPTLSGYIVQNYHWNVMFYGMFIIGIIAILVGFVWFKLYQYTTNPKADIPGIIFS
                     TIGFGALLYGFSEAGNKGWGSVEIETMFAIGIIFIILFVIRELRMKAPMLNLEVLKFP
                     TFTLTTIINMVVMLSLYGGMILLPIYLQNLRGFSALDSGLLLLPGSLIMGLLGPFAGK
                     LLDTIGLKPLAIFGIAVMTYATWELTKLNMDTPYMTIMGIYVLRSFGMAFIMMPMVTA
                     AINALPGRLASHGNAFLNTMRQLAGSIGTAILVTVMTTQTTQHLSAFGEELDKTNPVV
                     QDHMRELASQYGGQEGAMKVLLQFVNKLATVEGINDAFIVATIFSIIALILCLFLQSN
                     KKAKATAQKLDADNSINHE"
     gene            complement(9383..10030)
                     /locus_tag="POB55_001686"
     CDS             complement(9383..10030)
                     /locus_tag="POB55_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDI6500648.1"
                     /translation="MKKMILINVITVVVLLAIGIAGFYFWNKTTSYVTTDNAKVNGDQ
                     IKIASPASGQIKSLNVKQGDKLDKGDKVATVTVQGQDGETKDMDLKMPQKGTIAKLDG
                     MEGSMVQAGNPIAYAYNLDDLYVTANIDEKDIKDVEVGKDVDVTIDGQKASIKGKVDS
                     IGKATAASFSLMPSSNSDGNYTKVSQVIPVKITLESEPSKQVVPGMNAEVKIHKN"
     gene            10151..10702
                     /locus_tag="POB55_001687"
     CDS             10151..10702
                     /locus_tag="POB55_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDI6500649.1"
                     /translation="MKRQAKIEIQNALVDLMAEYPFQEISTKMICAYCNINRSTFYDY
                     YKDKFDLLDTINSKHKEKFQFLLSALHHNFENIKQDKLKLYKFFIIIAKYIKHNEQFF
                     KDILVTYPMKTLFIDYINLARDYYQQIMNDYSTTVTNKQLYVTYIIGGQAGVFINWLS
                     NGCNESPEEVADILLANTIKLQN"
BASE COUNT         3596 a         1892 c         1628 g         3819 t
ORIGIN      
        1 aatactattt tatcaaacat ttaccacaat aaaaatatct ttatcatttt tatttaattt
       61 aatcatataa ttgcgaggag aatattatgg atttcgttaa taatgataca agacaaattg
      121 ctaaaaactt attaggtgtc aaagtgattt atcaggatac cactcaaacg tatacaggct
      181 acatcgtgga aacggaagct tacttaggtt tgaatgatcg tgcggctcat ggctatggcg
      241 gtaaaataac acctaaagtc acgtcattat ataaacgtgg tggtacaatt tatgcacatg
      301 tcatgcatac gcatttactc attaattttg taacaaaatc tgaaggtata cctgaaggcg
      361 tacttatacg cgcaattgaa ccagaagaag gtttatccgc tatgttccgt aacagaggta
      421 agaaaggcta cgaggtaacg aatggcccag gaaaatggac taaggcattt aacattccac
      481 gggctatcga tggcgctacg ttaaatgact gtagattgtc tattgatact aagaatcgta
      541 aatatcctaa agatattatt gctagtccac gaatcggtat tccaaataaa ggtgattgga
      601 cacataaatc tttacgttac acagtgaaag gtaatccatt tgtgtctcgc atgcgtaaat
      661 cagattgtat gtttcccgaa gatacttgga aataaatgcc atctttcatt gattactatc
      721 atgaaaatga aatctatctc cttataagtc aatcaatcgt gccgtcaaca tgcggatggg
      781 ttgattgttt ttctttgtat ccatcatatt ttttgattca tctcctctta ttgaacttgt
      841 tcttaattat aaaatataac aatagaatta tttataatta ttaaatttag atgcattaat
      901 attattgata ttattttcaa aaactagaaa tattgatttg ttgcatgtat aatgttaaaa
      961 gcgccctttt ataacgctta catataaaag cttatttagg gagagggata ttcaacaagg
     1021 gggatttgaa aatgatagaa cttaatgcaa ttacaacatt atgtttagct tgtatccttt
     1081 atttacttgg taaggctatc gttaatcacg ttaatttttt aaaacgtatt tgtataccag
     1141 caccagtgat tggcggctta atctttgcta ttttagttgc ggctttggat tcatttggca
     1201 tggttaagat taaattagat gcttcattca ttcaagattt cttcatgtta gcattcttta
     1261 cgacaatcgg tcttggtgca tcattgaaat tatttaaatt aggtggcaaa ctcttgctat
     1321 tatactttat gttttgtgct atcatttcag tcattcaaaa catagttggt gtatcactag
     1381 caaaagtatt aaatattaaa cctttgttag gattaacagc aggttccatg tctatggaag
     1441 gcggtcatgg taatgctgct gcttatggta agacaattca agatttaggt attgattcgg
     1501 cactgacagc ggctcttgca gctgcaactt taggtcttgt atttggaggg cttatcggtg
     1561 gtccagttgt aaaattttta atcaaacgtt ataacttaaa gcctcaacat agtgacgata
     1621 catttaaaga ttatagccaa gtagcatata acgaacattt acatagtaaa tttaatgcca
     1681 ctgaagtatt cttcattcaa tttacaatcg ttgtattctg tatggcagtt ggaagttatt
     1741 tcagtcattt gtttacatct caaacaggga ttaatgttcc aatttacgtt ggctcattat
     1801 ttgtagctgt tattgtccga aatatctctg aaagttttaa ttttaatatt gtagatttaa
     1861 aaattactaa tcaaattggc gatgtcgcat taggtatttt cttatctctt gcgctaatga
     1921 gcattcaatt aatcgaaatt tataaacttg ctatacctct tattattatt gttttagttc
     1981 aagttgtcgt tatgatttta tttgctgttt taattttatt tagaggttta ggaaaagatt
     2041 atgatgctgc agtaatggta ggtggtttta tcgggcatgg gcttggtgca acgccaaatg
     2101 ccatggcaaa tttagatgtt attactaaaa aatatggtaa ctcacctaaa gcatatttag
     2161 ttgtacctat tgttggtgca ttcttaatcg atttaattgg tgttatagtc attatgggat
     2221 tcattcaatg gtttagttaa acatcaaact cataaataaa agaggaggtc ttcgcctcct
     2281 cttttattta tcctcgatgt atattcaagc tacgttgttc tatccatgac aatatttccg
     2341 gactaaatac gatttgtttt tgtgttaagt cgtcaatatt tttagcatct aacatcgtca
     2401 ttattgattt catgtgttca ataaatgatt ctacataagc tactgtatgt gcaatgccat
     2461 tattttcaac ttgatttaaa aacggacgag acataccagt tgcctttgca ccaagtgcta
     2521 aacttttaat tgcatcgagt ggtgtacgta aaccaccact cgcgaaaact gaaatttcgc
     2581 tttgataagc cgttgtttca agtaatgact caactgtaga ctgtccccat gatgataagt
     2641 aatccatatc tttatttgca cgacgttcat tttcaatatc tacaaagtta gtaccacctt
     2701 tgccactaac atcgacatac ttgacgccta tttgttgtaa gtcatgcatt aattctttgc
     2761 tcataccaaa tccaacttct tttataatga ctggaacaga cactcgtgat acaatcgacg
     2821 ctatattatc taaccaagtc acaaattcac gattcccttc aggcataact aattcttgag
     2881 gagaattaac atggatttgt aacgcttgtg cctcaagtaa ttcaactgct tccaaagcct
     2941 tttctactgg tacgtccgca ccaacattgc taaaaatcat gccttcagga ttcatttttc
     3001 gcgcaatcgt aaacgtctca gccatgcgtg gatttctcaa tgccgcatgt gttgatccaa
     3061 ctgccatcgc taagccagtt tctcttgcaa ctacagctag cttttcattg atgtttttcg
     3121 tccactcgct accacccgtc attgcattaa tataaaccgg atatgccatc gttaagtcag
     3181 gcgtctgtga tgtcaaatcg atatcattta cattaattga tgggatagaa tgatgcacaa
     3241 aacgcatctt atcaaaatct gaatacattg cgtcagattg agccattgct atttcaacat
     3301 gttcattttt tctctgttct ctttgaaaat cactcatgat taaacctacc ttttcgtcat
     3361 ttctaattta aaaccatttt ttacgtctga aaaagatgat taatacgaca gctataacaa
     3421 acattacagc taagcaaata taatatccat aatgtaaatg taatgccggc atatttacaa
     3481 agttcatacc ataaatccca gctatgaatg ttaacggtga aaatataact gatactaacg
     3541 ttaatacttg cataatacta ttcattctaa atgacgtgta tgactcaaaa ttttctcgta
     3601 tttcgtttgt catttcttga gcagtacgaa tgatattacg ttgcttaatc aagtggtcat
     3661 cgatatgttg aatgtatagc gaatgtttat tatctataat caaatcacca ttttgtttca
     3721 ttgtatcaat tagctcttgc ataggaaaca gtacacgttt tactttaatc aaatccgaac
     3781 gtaacttaaa gacactatcc atgaccattt tattaaagcg atcatctaca tggcggtctt
     3841 caaaatgata aacactatct tcaagtgcat atacaaagtt gaaatattta tcaaccatca
     3901 tatctaaaat taatatgacg acatctgcac aatctaattc tgcatctaat gtattcatat
     3961 acttatagac tactttattt aatgattcca acgtttgatg atgatatgtt actaatacat
     4021 tgtcttgtat aaaaatattt agtgctattg gtgaatagtt tgaccccata atactatgga
     4081 atactaagta ttgataatct ttataagatt tatatttagc tcgtggcata ccgttaattg
     4141 catcatccac ttctaaatca ttaaaattaa aatgtgcttt aaaccattca ttttcttgtt
     4201 cattcggttc atcaaaatca taccaaacaa tagtcgcatc ttttggtatc tctttgatat
     4261 catcaactac tttaaacggt tcatatgtag tttgataccg tatctttaaa gccatcgata
     4321 ctccccctaa ataacgaatt ctctattatt ttatcatgaa ttaaataacg tgtatgtctt
     4381 aatttatttt agtatgatag tcactaagga gatggttatt atcaaacaac tttttacaca
     4441 tactcaaacc gtaacatctg aattcattga ccataacaat catatgcatg atgcaaatta
     4501 taatatcatt tttagtgacg tcgtgaatcg ttttaattac agccacggtc tttctttaaa
     4561 agaacgcgaa aatttagcat atacgctatt tacactagaa gaacatacga catacctctt
     4621 agaattgtct cttggcgatg tatttactgt tactttatat atttatgatt acgattataa
     4681 gcggttgcat ttatttttaa cattaactaa agaagatggt acactagcat caacaaatga
     4741 agtaatgatg atgggaatta atcagcacac acgtcgttct gatgcttttc ctgaatcatt
     4801 ttcaacacaa atagcacact attataaaaa tcaaccaact atcacttggc ctgaacaatt
     4861 aggacataaa atagcaattc cacacaaagg agcattaaaa tgacagatgc attacaacaa
     4921 aagattcata tcgaattact agatttatta gatgatgtta agtttgaatt aacagaatta
     4981 aatgcacaaa aagggttata cattaacgga ccagcaaatc agctacttaa gcgtggcgtg
     5041 catatggctt atgttcaagg acaaaagcaa gccatcgata atattatgac tattgtggaa
     5101 caacagcttg aagatcaaca tttcctagaa cattatgata aatttcaaaa tgaggttgct
     5161 catcgcaatt atgataaaac agctaacttt gcagaactta gtgatattcc acgtcaattc
     5221 gataattttt tagatcaatt ttatcaaatt aaagggcaat actttatcat cacacatatc
     5281 aatacactta ttggtgattt tcactcagaa gctcattaac aattagtcta tataaccctt
     5341 gctatatttt caaaaacaaa acccaattac gatttcatgt caaatatcat cttgcatgaa
     5401 atcgtaactg ggtcatttat atgttattag ttattttgtg ttacatcatc atctatcgat
     5461 ttggcaattt gtttaatagc tttatgtgat tgtctaattg gataaattgg aaaatcatgt
     5521 accatcttag gataatcata aaactcaatg tattgatgat gttgcaacat catttgttca
     5581 aatagcttca tatcaggatg tgtcatttca cgtccaccac caaacatata aactggtggc
     5641 aatccttcta ttgtgccatt aattggcgat atgcgcttat ctgttaatgg taggccattc
     5701 gcccattttt tcataatctc attgacacca aactgactta gaaccgcatc ttgttcgatt
     5761 aaggcgtccg aaatatcttt attagatagt gttgcatcta aaattggtga gattaaatac
     5821 aatttattcg gtaatggctg ttgattatct aaaagagatt gtacaaagga taatgccagt
     5881 gcaccacctg aaccatcacc catgactacg acattttgag gtcctacttc agatactaat
     5941 tgatcataaa cacgttgtat cgcttggaaa gtatcgtcaa tatgaaactc tggtgtcttt
     6001 ggatagatag gcagtacaac ctcatataat gtacttaaag tgattttatc caacaatctc
     6061 caatggaacg gtgatggttg tagtgcattg aatccaccgt gaatatataa aattttctta
     6121 tcaatttgat gtctgaaatt aaagcgaaag acttgcatat catctaatga taatttttct
     6181 aaatttgctt taacatttaa tgttgaaggc tgcttatgtt tttttctatt ttcaatttct
     6241 cttttataaa aaaatctttc aacatcttga tcatttttaa acataatcga gcgattgtga
     6301 agcaaatatt tattgacaac gctattcata acacggtttc taatcaatgt cttaacctac
     6361 ctttatatat tttatgtatc caatgattgt ctatccccta cattctttgc caaaaaaagt
     6421 atataatgta gaagatattt tctttttcac tttcaaattt aagactacaa ttgaacagtg
     6481 atttttcatc attataacag acaactagac atattgataa gtaaagaaaa gaactttata
     6541 cggaggtacc ttgcatgaca aatccaaatc aacgattaga accatttgat gagacatttc
     6601 aacaatcgaa tattcatcgt ggtaagcgat atggtaagaa aaaacgttca ttggtaagca
     6661 tgattattca aatcattgtt gttatattaa ccgcaatcgc tggatacagc atgtggaaac
     6721 aacccatttt taatattgtc ttcaccaaag aatctgtaga ttttgatggc attaaaaatt
     6781 ttaacgagac aattggaaaa atgcatcaat taaatattaa tttgggtaat atcaatgatt
     6841 tacaacaagg aatcgatcgc ttaattttag tctttaacgg tttctttatt ttatgtatcg
     6901 ttagcctagt cataacaata ctgacaattg tatttaatcg cacagttcta aaagtagtaa
     6961 atattttagt gttaacaatt atgttagtca ttacattgta tttcagttat attattaaag
     7021 cactcgcaca agaaattgca gatgctttaa aaaaatatta tttaaatgta ccaccagatc
     7081 aagtgattac agaagctgac gctattcata acggcatcat tttactgggc tgtagcatcg
     7141 gtttacttgt tataagctta ttcttccgta atcgcatgcc ccgtttaaaa taatgttata
     7201 tagtagggat tgggacagaa atgttatttt cacaaaattc atttcgttga cccacttcct
     7261 gctttttctt tattaatgac ctactacgtc aatgccaaat tttaatataa ttcctctaac
     7321 attctacaaa tcaacaaata aaaaaagtat cgataaccat atttggcacc gatactttaa
     7381 aaatcatgtc cccacaacat aatttttgat tagtcacact tcaattaatt tattttattt
     7441 attcatgatt gatactatta tctgcatcta acttttgagc tgtagctttt gcttttttat
     7501 tactttgtaa aaataaacat aaaattaagg cgatgatgct aaatatcgtt gcaactataa
     7561 atgcatcatt aataccttca accgttgcta gtttatttac aaattgtagt aacactttca
     7621 ttgcgccttc ttgtccgcca tattgtgatg ctaattcacg catatgatct tgtacaacag
     7681 gattcgtttt atctaactct tccccaaaag ctgataagtg ttgtgtagtt tgtgttgtca
     7741 ttacagtaac taagattgct gtacctatag agcctgctaa ttgacgcatc gtatttaaga
     7801 aagcattacc atgagaggca agtcgtcccg gtaacgcatt aatagctgca gttaccattg
     7861 gcatcattat aaatgccata ccaaatgaac gaagtacata gatacccatg attgtcatat
     7921 atggtgtatc catatttaat ttagttaatt cccatgttgc ataagtcatg acagcaatac
     7981 caaaaatggc taaaggtttt aaaccaatcg tgtctaacaa cttaccagca aatggtccta
     8041 gtagacccat aattagagaa ccaggtaata ataacaatcc ggaatctaat gctgagaaac
     8101 cacgtaaatt ttgtaagtaa attggtaata aaatcatacc accatacaaa cttaacatta
     8161 caaccatatt aataattgtt gttaatgtaa atgttgggaa tttcaatact tctaaattca
     8221 acattggcgc cttcattctt aattctctta ttacaaatag aataataaag ataataccaa
     8281 tcgcaaacat tgtttctatc tcaacagaac cccagccttt gttccccgct tctgaaaagc
     8341 cgtataacaa agcaccaaaa ccaatcgtac taaaaatgat acctgggata tcagctttag
     8401 ggtttgttgt atattgatat aacttaaacc atacaaaacc aactaaaata gcgataatcc
     8461 cgataatgaa cataccgtaa aacatcacat tccaatggta attttgtaca atataacctg
     8521 ataatgttgg accaattgca ggtgctaaaa tcattgcgat acccattgta cccatggcag
     8581 caccacgttt ttcaggtgga taaattgtaa taataacaat tgaacctaat ggcattagta
     8641 cacctgcacc aatggcttgt aatacacgtc caaccatcat gattgggaaa ttcattgaaa
     8701 tcgcacagat taatgaacca attgtaaaga gtactaacgc aactaaaaat aattttcgat
     8761 atgaatattt attaaataga tacgccgtaa ttggtattaa aataccgttt actaacatga
     8821 atcccgtcat caaccattgc cctgttgacg cagaaatatt aaattccgta ttaatttttg
     8881 gcaaagcaac atttaataat gtttggttta aaatcgcaat aaacataccg aataataatg
     8941 ccgctaatat tttaccgcgt gaaacacctt caccaaaaat aaagttttta tgttcttttt
     9001 ttattttttc attcacttta tattcttctg attcaggatt tttagcagca actgcttcct
     9061 caccattatt attagtgaat gcttcttgat ctttctcaga cccctttgtg taaccattta
     9121 gactaacttg gctatgatca tcttgattgt caacacgcaa ctcttcatgc gtcatacgtt
     9181 gtgtcgattg atcagttgtt ttgtctaaat cactagcttt aaatttagat tgatttgatt
     9241 gacgtgtcgt aaattgttgt tccttttgtt ggcgtttgcc tttttttctt gatcttatta
     9301 aaaataaatt gataacccca acaataatga gcgctaaaat aatgtagcta ataatgaagg
     9361 tcgtagtcat ttaatgaccc ccttaatttt tatggatttt tacttcagcg ttcattccag
     9421 gaacaacttg tttagacggt tctgattcta gagtgatttt aacaggtatt acttgagaaa
     9481 ctttagtgta gttaccatca ctatttgatg atggcattaa tgaaaagctt gcagcagttg
     9541 cttttccaat actatcaact ttacctttaa tagaagcttt ttgaccgtca atagtcacat
     9601 caacatcttt acctacttca acatctttaa tatctttttc gtcaatattt gctgttacat
     9661 ataaatcatc taaattgtat gcataagcga ttgggttacc agcttgcacc attgaacctt
     9721 ccataccatc taatttggca attgtacctt tttgaggcat tttaagatcc atatctttcg
     9781 tttcgccatc ttgaccttgt acagtaacag ttgctacttt gtcaccttta tcgagtttgt
     9841 caccttgttt aacattaagt gatttaattt gtccagatgc aggacttgct attttaattt
     9901 gatcgccatt tacttttgca ttatcagttg ttacatagct tgttgtttta ttccagaaat
     9961 aaaagccagc aatcccaatt gctaacagta caacaaccgt aatgacatta attaatatca
    10021 tctttttcat gaaacatttc ctcctatttg cttttaaaat tcactttatt tattatagag
    10081 acatgataac taaaaaaaca ccaacaattt ttataagagt gttggttaaa attcacaaag
    10141 gtgtaacaat atgaaacgac aagccaaaat agaaattcaa aacgcacttg ttgatttgat
    10201 ggctgaatat ccttttcaag aaatatccac taaaatgatt tgcgcgtatt gtaatattaa
    10261 tcgttctact ttttatgatt attacaaaga taaatttgat ttattggata ccatcaactc
    10321 aaagcataaa gaaaaattcc aatttcttct aagcgcgcta catcataatt tcgaaaatat
    10381 taaacaggat aagcttaaac tttataaatt cttcattatt attgctaagt atatcaagca
    10441 caatgagcaa tttttcaaag acattttagt tacctatcca atgaaaacat tattcataga
    10501 ctatataaat ttagcgagag attactatca acaaattatg aatgactata gtactacagt
    10561 aaccaataaa cagctttatg ttacatatat cataggcggt caagctgggg tgtttattaa
    10621 ttggctttcg aatggttgta atgaatcacc tgaagaagtt gctgacattt tattggctaa
    10681 tacgattaaa cttcaaaact aataatacgg tttcattcta ataaagaaat gctataaaac
    10741 aactagataa agttaaacca aatggataga taatagtaca tatgttacgg tgctatcaat
    10801 ataaaaatgc cgagatttcc acataataat ctcggcattt ttatttttga tgctaaattc
    10861 ataggtctat gaatagctca agttttgttt tttaattttt caattcaata taatagaaga
    10921 aactatgttt atcat
//
LOCUS       DAMZKC010000089        10821 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.090, whole genome shotgun sequence.
ACCESSION   DAMZKC010000089 DAMZKC010000000
VERSION     DAMZKC010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10821
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.090"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(<1..294)
                     /locus_tag="POB55_001688"
     CDS             complement(<1..294)
                     /locus_tag="POB55_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HDI6500650.1"
                     /translation="MLKKWLGMALITPILTFIIWLFNSHTIITYLNILFYVSLIIFIS
                     IFLILLVQEGIFDATSYGFRRLKYQMSSSKKKKSISDDPFFNPQEVKKEHYFVS"
     gene            537..1463
                     /locus_tag="POB55_001689"
     CDS             537..1463
                     /locus_tag="POB55_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6500651.1"
                     /translation="MGKYIFKRFIYMLISLFIIITITFFLMKLMPGSPFNDAKLNAEQ
                     KEILNEKYGLNDPVATQYLHYLKNVVTGDFGNSFQYHNQPVWDLIKPRLLPSFEMGLT
                     AMFIGVILGLILGVAAATKQNSWVDYTTTVISVIAVSVPSFVLAVLLQYVFAVKLRWF
                     PVAGWEGFSTAVLPSLALSAAVLATVARYIRAEMIEVLSSDYILLARAKGNSTMRVLF
                     GHALRNALIPIITIIVPMLASILTGTLTIENIFGVPGLGDQFVRSITTNDFSVIMAIT
                     LLFSTLFIVSIFIVDILYGVIDPRIRVQGGKK"
     gene            1463..2533
                     /locus_tag="POB55_001690"
     CDS             1463..2533
                     /locus_tag="POB55_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6500652.1"
                     /translation="MAENKNNLSINDDHSNAAMTHTSDAISSSDFIIRELDLNQEPEM
                     QRESKNFWQDAWAQLKRNKLAVVGMIGLIIIVIFAFIGPVINKHDYAEQNVEHRNLPA
                     KIPVLDKVPFLPFDGKDADGKDAYKAANAKENYWFGTDQLGRDLWTRTWKGAQISLFI
                     GVVAAMLDIFIGVVYGAISGFFGGRVDTIMQRILEVIASIPNLIVVILFVLIFEPSIW
                     TIILAMSITGWLGMSRVVRGEFLKLKNQEFVMASKTLGASKFKLIFKHILPNTLGAIV
                     VTSMFTVPSAIFFEAFLSFIGIGVPAPQTSLGSLVNDGRAMLLIYPHELFIPAMILSL
                     LILFFYLFSDGLRDAFDPKMRK"
     gene            2549..3631
                     /locus_tag="POB55_001691"
     CDS             2549..3631
                     /locus_tag="POB55_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6500653.1"
                     /translation="MTERILEVNDLHVSFDITAGEVQAVRGVDFYLNKGETLAIVGES
                     GSGKSVTTKAITKLFQGDTGRIKKGEILFLGEDLAKKPENELIKLRGKDISMIFQDPM
                     TSLNPTMQIGKQVMEPLIKHKNYSKAQAKKRALEILNLVGLPNAEKRFKAYPHQFSGG
                     QRQRIVIATALACEPKVLIADEPTTALDVTMQAQILDLMKELQQKIDTAIIFITHDLG
                     VVANIADRVAVMYGGQMVETGDVNEIFYDPKHPYTWGLLSSMPDLSTTNDTPLLAIPG
                     APPDLLHPPKGDAFARRSQYALDIDFKVEPPWFKVSPTHFVKSWLLDARAPKVELPEL
                     VKQRMKPMPNNYEKPLKVERVSFNEK"
     gene            3621..4562
                     /locus_tag="POB55_001692"
     CDS             3621..4562
                     /locus_tag="POB55_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6500654.1"
                     /translation="MKNDEVLLSIKNLKQYFNAGKKNEVRAIENISFDIYKGETLGLV
                     GESGCGKSTTGKSIIKLNDITSGEILYEGIDIQKIRKRKDLLKFNKKIQMIFQDPYAS
                     LNPRLKVMDIVAEGIDIHHLATDKRDRKKRVYDLLETVGLSKEHANRYPHEFSGGQRQ
                     RIGIARALAVEPEFIIADEPISALDVSIQAQVVNLLLKLQRERGITFLFIAHDLSMVK
                     YISDRIAVMHFGKIVEIGPAEEIYQNPLHDYTKSLLSAIPQPDPESERSRKRFSYIHD
                     EANNHLRQLHEIRPQHFVFSTEEEAAQLRENKLVTQN"
     gene            4581..6236
                     /locus_tag="POB55_001693"
     CDS             4581..6236
                     /locus_tag="POB55_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDI6500655.1"
                     /translation="MTRKFRTLILILIATIALSGCANDDGIYSDKGQVFRKILSSDLT
                     SLDTSLITDEISSEVTAQTFEGLYTLGKGDKPVLGVAKAFPEKSKGGKTLKVKLRSDA
                     KWSNGDKVTAQDFVYAWRKTVDPKTGSEFAYIMGDIKNASDISTGKKPVEQLGIKALD
                     DETLQIDLEKPVPYINQLLALNTFAPQNEKVAKKYGKNYGTAADRAVYNGPFKVDDWK
                     QEDKTLLSKNQYYWDKKNVKLDKVNYKVIKDLQAGASLYDTESVDDAVITADQVNKYK
                     DNKGLNFVLTTGTFFVKMNEKQYPDFKNKNLRLAIAQAIDKKGYVDSVKNNGSIPSDT
                     LTAKGIAKAPNGKDYASTMNSPLKYNPKEARAHWDKAKKELGKNEVTFSMNTEDTPDA
                     KISAEYIKSQVEKNLPGVTLKIKQLPFKQRVSLELSNNFEASLSGWSADYPDPMAYLE
                     TMTTGSAQNNTDWGNKEYDQLLKVARTKLALQPNERYENLKKAEEMFLGDAPVAPIYQ
                     KGVAHLTNPQVKGLIYHKFGPNNSLKHVYIDKSIDKETGKKKK"
     gene            6448..8163
                     /locus_tag="POB55_001694"
     CDS             6448..8163
                     /locus_tag="POB55_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDI6500656.1"
                     /translation="MGKLIKYISILLIVVLVLSACGKSSNKDEGVKDATKTEASKHKG
                     GTLNVALTAPPSGVYSSLLNSTHADAVVEGYFNENLLTIDKKIRPKSYIASWKDIEPA
                     KKIEFKIKKGIKWHDGNELKIDDWIYSIEVLANKDYEGAYYPSVENIQGAKDYHEGKA
                     DHISGLKKIDDYTMQVTFDKKQENYLTGFITGPLLSKKYLSDVPIKDLAKSDKIRKYP
                     IGIGPYKVKKIVPGEAVQLVKFDDYWQGKPALDKINLKVIDQAQIIKAMEKGDIDVTN
                     DATGAMAKDAKSSNAGLKVLSAPSLDYGLIGFVSHDYDKKANKTGKVRPKYEDKELRK
                     AMLYAIDREKWIKAFFNGYASEINSFVPSMHWIAADPKELNDYKYDPEKAKKILDKLG
                     YKDRDGDGFREDPKGNKFEINFKHYSGSNPTFEPRTAAIKDFWEKVGLKTNVKLVEFG
                     KYNEDLANASKDMEVYFRSWAGGTDPDPSDLYHTDRPQNEMRTVLPKSDQYLDDALDF
                     DKVGIDEKKRKDIYVKWQKYMNDELPGLPMFQGKSITIVNDKVRNLDIEIGTDQSLYN
                     LTKEA"
     gene            8214..9200
                     /locus_tag="POB55_001695"
     CDS             8214..9200
                     /locus_tag="POB55_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6500657.1"
                     /translation="MNNVLLEVKDLETSLKINNEWLATVENISFELSKGEVLGIVGES
                     GCGKSILSKSIIKLLPEKISKLSNGEVIFDGKRIDTLNEKQLLDIRGNDIAMIFQEPM
                     TALNPVFTIKNQLVESIKSHKKISKKEANNLAKDLLKKVGIARQDEILNSYPHQLSGG
                     MRQRVMIAMAISCSPKLLIADEPTTALDVTIQAQILDLLKELQKETQMAIMMITHDLS
                     VVAEFCDKVLVMYAGQIVEFGGIKEILHNPKHPYTQKLLSTIPKLKEEQKRLETIEGI
                     VPSIQAFHVNKCRFANRCNKKMDICNNQSPKMHVCEDVIVRCHLYKNEYKEI"
     gene            9203..10183
                     /locus_tag="POB55_001696"
     CDS             9203..10183
                     /locus_tag="POB55_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HDI6500658.1"
                     /translation="MENILEVNQIKKYYKIKTGLLQKTQYVKAVDDVSFSIKKGQTFG
                     LVGESGCGKSTLGKVIIRLEDATSGSIIVNGEDITRLQGKKLRKSRQQYQMIFQDPYA
                     SLNPMQMVGDIISEPILNYKKLPKEEIKKEVLYLLKCVGLSEDAYYKYAHEFSGGQRQ
                     RVGIARALALRPSLIVADEPVSALDVSVQSQVLNLLKDLQEQFNLSYLFIAHDLSVVK
                     HISDVIGVMYLGHIVEIASDKEIYENPKHPYTKALISSIPQIDKHNNNRIILKGELPS
                     PSNPPQGCPFHTRCPIAKDKCKENIPQLKDIGDEHQVACFYVNKVGDLNG"
     gene            10176..>10821
                     /locus_tag="POB55_001697"
     CDS             10176..>10821
                     /locus_tag="POB55_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6500659.1"
                     /translation="MVKLILKRLGLMIPLLILISIVVFSLAIIQPGDPFSDLQNGKIK
                     QEAINAQREKLGLNDSISHQYIRWVNHVIHGDLGESIKYKRPVIDVIEERIPNTILLG
                     AMSLIITYIISFALGITSGRYSYSLTDYTVQIFNYLMLAIPSFIAGVFAIFIFSFELQ
                     WFPFQGSVDINLKEGTFEYYMSKIYHTFLPALTLGLLSTAGYIQYLRNDIIENSK"
BASE COUNT         4027 a         1469 c         1988 g         3337 t
ORIGIN      
        1 agaaacaaag taatgctctt tttttacctc ttgtgggttg aaaaatggat catcagagat
       61 agacttcttc tttttcgaag atgacatttg atactttaat cttctaaaac cataacttgt
      121 cgcatcaaaa atgccttctt gtacaagtaa aatcaaaaat atgctaataa aaataattaa
      181 tgaaacataa aacaatatat ttaaatatgt aatgatagta tggctattaa aaagccatat
      241 aataaacgtt aatattggcg ttattagtgc cattccaagc cattttttca acatttgatc
      301 actcccactt atagaaaact cttacgcata gtttacatta aaatcagaca ttgaggaatg
      361 attttttaat ttcttcagct ttattgaaat tctaaaatca atcattcttc attagtttaa
      421 agcaaaaaaa tattgatata tagtaaatat tgtatatata atattagtta agatttcaga
      481 aaattttgaa gggaatggaa atttagaaat cggaatttgt tagaggaggg gattagatgg
      541 ggaaatatat tttcaaacga tttatttata tgcttatttc tttatttatt attattacaa
      601 ttacattttt cttaatgaaa ttaatgccag gttcgccatt taacgatgct aaattaaatg
      661 ctgaacaaaa agaaatttta aatgaaaaat atggattaaa tgatcctgta gctacgcagt
      721 atttacatta tttaaaaaat gttgttacag gcgattttgg taattcattc cagtatcata
      781 atcaacctgt gtgggatttg attaaaccga gactactacc ttcttttgaa atgggtctta
      841 cagcaatgtt catcggtgtg atactgggac ttattttagg tgttgcagca gctactaaac
      901 aaaattcttg ggttgactat acaactacag ttatttcagt tattgcagta tctgtaccat
      961 cttttgtact tgctgtactt ttacaatatg tatttgcagt taaattaaga tggttcccag
     1021 tagctggatg ggaaggtttt tcgaccgcgg tattaccgtc acttgcatta tctgcagctg
     1081 ttttagcaac tgtcgccaga tacataagag cagagatgat agaggtatta agttcagact
     1141 atattttatt agcgagagct aaaggtaatt cgacaatgcg tgtacttttt ggacatgcac
     1201 ttagaaatgc tttaattcca attattacaa ttatcgttcc catgttagca agtattttaa
     1261 caggcacttt aacaattgaa aatatttttg gggttcctgg attaggggat caattcgtac
     1321 gttcaattac aacaaatgat ttctcagtaa tcatggcaat cacactatta tttagcacac
     1381 tgtttatcgt ttctattttt attgtagata ttttgtacgg tgtgatagat ccacgaattc
     1441 gtgttcaagg aggtaaaaaa taatggctga aaataaaaac aatttgtcga ttaacgacga
     1501 tcattctaat gcagctatga cgcatacctc tgacgctatc tcatcatctg attttattat
     1561 tagagaatta gatttgaatc aggaacctga aatgcaacga gaaagcaaaa acttttggca
     1621 agatgcttgg gctcagttaa aacgaaataa gttagctgtt gtcggtatga taggtttaat
     1681 tatcattgta atatttgctt ttatcggtcc agttataaat aaacatgatt atgctgaaca
     1741 aaatgtagaa catagaaatc ttccggcaaa aatacctgta ttagacaaag ttccattttt
     1801 accttttgat ggtaaagatg cagatggcaa ggatgcttat aaagcagcaa atgctaaaga
     1861 aaattattgg tttggtactg atcagttggg tcgagattta tggacaagaa catggaaagg
     1921 tgctcaaatt tcattgttta tcggtgttgt tgcagcgatg ttagatattt ttattggcgt
     1981 tgtatatggt gcgatttctg gattcttcgg tggacgtgtc gatacgatta tgcaacgtat
     2041 acttgaagtc atagcatcta ttccgaattt aattgtcgta attttatttg tattaatttt
     2101 tgaaccatcc atttggacaa ttatattggc tatgtctatc acaggctggt taggcatgag
     2161 cagagttgta cgtggagaat ttttaaaatt aaaaaatcaa gagtttgtca tggcttcgaa
     2221 aacattgggg gcttcaaaat tcaaattgat atttaagcat attttaccta atacattagg
     2281 tgctatcgtg gttacatcaa tgtttacagt acctagtgct attttcttcg aagcattttt
     2341 aagtttcatt ggtataggtg tacccgcacc tcaaacatcg ttagggtcat tagtaaatga
     2401 tgggcgagca atgttattaa tttatccaca tgaattattt ataccagcaa tgattttaag
     2461 tttattaatt ctattctttt acttatttag tgatggatta cgtgatgcat ttgatccgaa
     2521 aatgcgtaaa taaaaggggg catagcatat gactgaaaga atattagaag taaatgattt
     2581 gcatgtttcc tttgatatta cagcagggga agtgcaggca gtgagaggtg tagattttta
     2641 tttaaacaag ggggaaacat tggcaattgt tggtgaatca ggttcaggta aatctgtaac
     2701 aacaaaagca attacaaaat tattccaagg ggacacagga agaattaaaa agggagaaat
     2761 tttattttta ggggaagatt tagcaaaaaa acctgaaaat gagttaatta aattacgtgg
     2821 caaagatatt tcaatgatct ttcaagatcc aatgacatct ttaaacccaa ctatgcaaat
     2881 tggtaaacaa gtcatggaac cattaattaa gcacaaaaat tatagtaaag cacaagctaa
     2941 aaagcgcgca ttggaaatac taaatcttgt aggtttacca aatgcagaaa aaagatttaa
     3001 agcatatcca catcaatttt caggtggaca aaggcaaaga attgttattg caaccgcatt
     3061 agcttgtgaa cctaaagtgc tcattgctga tgaaccaacg actgcattag acgtaacgat
     3121 gcaggcacaa attttagatt taatgaaaga actacaacaa aaaatcgata ctgcaattat
     3181 ttttataacg catgatttag gggttgttgc gaatattgct gatagagtgg cagttatgta
     3241 tggtggtcaa atggttgaaa caggagatgt taacgaaata ttttatgatc caaagcatcc
     3301 atatacatgg ggattattat cgtcaatgcc tgatttatca acaacaaatg acacaccatt
     3361 actagcgatt cctggagcgc cacctgattt attacaccca cctaaaggtg atgcatttgc
     3421 gagacgtagt caatatgcat tagatattga ttttaaagta gaaccaccgt ggtttaaagt
     3481 ttcaccgaca cattttgtga aatcttggtt attagacgca cgtgcaccaa aagttgaact
     3541 acccgagctg gtaaaacaac gtatgaaacc gatgcctaat aattatgaaa aaccactcaa
     3601 ggtagaaagg gtgtcgttca atgaaaaatg atgaagtgct attatccatt aaaaatttaa
     3661 agcaatattt taacgcagga aagaaaaacg aagtgagagc gattgaaaat atttcgtttg
     3721 atatatacaa aggggaaaca ttaggtttag taggagaatc ggggtgtggt aaatctacaa
     3781 ctggtaaatc aattattaaa cttaatgata ttacaagtgg agaaattttg tatgagggta
     3841 ttgatataca aaagattcgt aaacgtaaag atttgcttaa atttaataaa aagatacaga
     3901 tgatttttca agacccatat gcgtctttaa atcctaggtt aaaagtaatg gatatagtag
     3961 ctgaaggtat tgatatccat catttagcaa ctgataaacg tgaccgtaaa aaacgtgtct
     4021 atgatttact tgaaactgtt ggattaagta aagaacatgc caatcgctat cctcatgaat
     4081 tttcaggtgg acaacgccaa cgtattggaa ttgcccgtgc attagccgtt gaaccagaat
     4141 ttattatcgc ggacgaacca atatcggcat tggatgtttc aatccaagct caagtagtta
     4201 atttattatt aaaattacaa cgtgaaagag ggattacgtt cctatttata gctcatgatc
     4261 tatcaatggt gaagtatatt tcagatcgta ttgcagtgat gcattttggg aaaatagttg
     4321 aaattggacc ggcagaagaa atttatcaaa atccattaca cgattatact aagtctttat
     4381 tatcagccat tccacaacct gatcctgaat cagaacgcag tcgcaaacga tttagttata
     4441 ttcatgatga agcaaataat catttaagac aattacatga aataagacca cagcattttg
     4501 tctttagtac tgaagaagaa gcggcacaac tacgagaaaa taaattggtg acacaaaatt
     4561 aaggggaagg gggaaatgca atgacgagaa aatttagaac acttatttta attttgattg
     4621 ctacaattgc attaagtggt tgtgctaatg atgatggtat ttattcagat aaaggtcaag
     4681 tattcagaaa aattttgtca tcagacttaa catcccttga tacatcatta ataacggatg
     4741 aaatatcttc tgaagtgact gcgcaaacat tcgaaggttt atacacatta ggaaaaggtg
     4801 acaaaccggt gttaggtgtt gcgaaagctt ttcctgaaaa gagtaaaggt ggtaaaactt
     4861 taaaggttaa attaagaagc gatgctaaat ggagcaatgg tgacaaagtg actgcgcaag
     4921 attttgttta tgcttggaga aaaacagttg accctaaaac aggttctgaa tttgcataca
     4981 ttatggggga cattaaaaat gcgagtgata ttagtactgg taagaaacct gtagagcaat
     5041 taggtatcaa agcattagat gatgaaacat tacaaattga tttagaaaag ccggttccat
     5101 atattaatca attattagca cttaatacct ttgcacctca aaatgaaaaa gttgccaaaa
     5161 aatatggtaa aaattacggt acggcagctg atagagcggt atacaatggt ccatttaaag
     5221 ttgatgattg gaaacaagaa gataaaacgt tactatctaa aaatcagtat tattgggata
     5281 aaaagaatgt aaaattagat aaagtgaatt ataaagttat taaagactta caagccggtg
     5341 catcattgta tgatactgaa tcagtagatg acgcagttat tactgcagat caagtaaata
     5401 aatataaaga taacaaagga ttaaactttg tgttaacgac tgggacattt tttgtaaaaa
     5461 tgaatgaaaa acaatatcct gattttaaaa acaaaaattt aagactggct atcgcacaag
     5521 caatagataa aaaaggatac gttgattcag tgaaaaacaa tggctcaatt ccttccgata
     5581 cactaacagc caaaggaatt gcgaaagcgc ctaatggcaa agattatgcg agtaccatga
     5641 attcgccttt aaaatataat cctaaagaag caagagcaca ctgggacaaa gctaaaaaag
     5701 agttaggtaa aaatgaagtg acattttcaa tgaacacaga agatacacca gatgcaaaaa
     5761 tatctgctga atatatcaaa tcgcaagttg agaaaaattt accaggagtt actttgaaaa
     5821 ttaagcaatt accgtttaaa caaagagtat cactagaact gagtaacaat tttgaagcat
     5881 cacttagtgg ttggtctgca gattaccctg atcctatggc ttatttagaa acaatgacca
     5941 caggtagcgc acaaaataat acagactggg gtaataaaga atatgatcaa ttacttaaag
     6001 tagcaagaac caaattggca cttcaaccga acgaacgata tgaaaacttg aaaaaagcag
     6061 aagaaatgtt cctaggagat gcaccggtag caccaattta tcaaaaaggt gttgcacatt
     6121 taacaaatcc tcaagtaaaa ggattaattt accataaatt tggtccaaat aactcactta
     6181 aacatgtata tattgataaa tcgatagata aagaaacagg taagaagaaa aaataatatg
     6241 ctttgtaaat taggctggag acatatctcc agtctttttg tgttggataa aaactttggg
     6301 aataaaaatt taaaataagt cgttttttaa attactgaaa ttgattaaat gcataaataa
     6361 ctgaatattc taaaaataaa cttgtaataa ttttttctat gagtaaacta aaaagaaaaa
     6421 attagattga aagtaggagg catatgtatg gggaagctta ttaaatatat ttcaatactt
     6481 cttattgtcg ttttagtgtt gagcgcttgc ggaaaaagca gtaataaaga tgaaggagta
     6541 aaagatgcta ctaaaacaga agcgtcaaaa cataaaggtg gtacgctaaa tgtagcgtta
     6601 acagcaccgc caagtggtgt ttattcttcg ttattaaata gtacacatgc ggatgctgta
     6661 gttgaaggat attttaatga aaacttatta acaattgata aaaaaatacg tcctaaatca
     6721 tatattgctt catggaagga catcgagccg gctaagaaaa tagaatttaa aattaaaaaa
     6781 ggtattaaat ggcatgatgg taatgaattg aaaattgatg attggattta ttcaattgaa
     6841 gtcttagcta acaaggatta cgaaggtgct tattatccaa gtgtagaaaa tatccaaggt
     6901 gcaaaagatt atcatgaagg aaaagctgat catattagcg gattgaagaa aatagatgac
     6961 tacacaatgc aggttacatt tgataaaaag caagaaaatt acttaacagg atttattact
     7021 ggacctttat taagtaaaaa atatttatca gatgtaccaa ttaaagattt agcgaaatca
     7081 gataaaatcc gaaaatatcc tattggtatt ggaccatata aagttaagaa aatcgtccca
     7141 ggtgaggctg ttcaacttgt taaatttgat gattattggc aaggtaagcc tgcattagac
     7201 aaaatcaatt taaaagttat tgatcaagct caaattatta aggcaatgga aaaaggcgat
     7261 attgatgtta cgaatgatgc taccggtgca atggcaaaag atgctaagtc atctaatgct
     7321 ggtctcaagg tattatctgc gccaagctta gactacggtt taataggatt cgtatctcat
     7381 gattacgata aaaaagctaa taaaactggt aaagtgagac caaaatatga agacaaagaa
     7441 ttacgtaaag caatgcttta tgcaattgat agagaaaaat ggatcaaagc atttttcaat
     7501 ggttacgcta gtgaaataaa tagttttgta ccatcaatgc attggatagc agctgatcct
     7561 aaggaactaa acgattataa atatgatcct gaaaaagcta aaaaaatctt agataagtta
     7621 ggttataaag atcgagatgg tgacggattt agagaagatc ctaaaggtaa taaatttgag
     7681 attaacttta aacattattc aggttcaaat cctacttttg aaccaagaac tgctgcgata
     7741 aaagatttct gggaaaaagt tggcttgaaa acaaatgtga agttagtaga attcggtaaa
     7801 tataatgaag acttagcaaa tgcatcaaaa gatatggaag tgtacttcag atcatgggca
     7861 ggtggtacag atccagaccc atcagattta taccacactg atagacctca aaatgaaatg
     7921 agaacagttt taccaaaatc agatcaatat ttagatgatg cgttagactt cgataaagta
     7981 ggcattgatg aaaagaaacg taaagatatt tatgttaaat ggcaaaaata tatgaatgat
     8041 gagttacctg gattaccaat gttccaaggt aaatcgataa ctatagttaa tgataaagta
     8101 cgaaacttag acattgaaat tggaactgat caaagtttat ataatttaac taaagaagct
     8161 tagtaatgta acctcgacga ttttgtcgag gtttacttat atggggggat gttatgaata
     8221 atgtattgtt agaggttaaa gatttagaaa catcattaaa aataaataat gaatggttag
     8281 caactgttga aaatatttct tttgaattat ctaaaggaga agttttgggt atagtagggg
     8341 aatctggttg cggtaagtcc atattaagta agtcaattat taaattatta ccagaaaaga
     8401 tatctaaact aagtaatgga gaagttatat ttgatggtaa acgaatcgat acgctcaatg
     8461 agaagcaatt gctagatatt cgaggaaatg atattgctat gatttttcaa gaacctatga
     8521 ctgctttaaa tcctgtattt accataaaaa atcaacttgt ggaatctata aaatcacata
     8581 aaaaaatttc taaaaaagaa gcaaataact tagcaaaaga tttactaaaa aaagttggaa
     8641 ttgctagaca agatgaaata ttaaatagct atcctcatca attatctggt ggtatgagac
     8701 aaagagtaat gattgcaatg gccatttcat gttctcctaa attattaatt gctgatgaac
     8761 ctacaacagc attggatgtc acgattcaag cgcaaatatt agacttatta aaagaattgc
     8821 aaaaggaaac gcaaatggca attatgatga ttacacatga tttgagtgta gttgctgagt
     8881 tttgcgataa agtcttagtt atgtatgcag gtcaaattgt agaatttgga ggcataaaag
     8941 aaatactaca caatccgaaa catccttata cccaaaaatt attatcaaca attccaaaac
     9001 ttaaagaaga gcagaaacga cttgaaacga tagaaggaat tgtgccatca atccaagcat
     9061 ttcacgttaa taagtgcaga tttgcaaata gatgtaacaa aaaaatggat atttgtaata
     9121 atcaatctcc taaaatgcat gtttgtgaag acgtcattgt acgttgtcat ttgtacaaaa
     9181 atgaatataa ggagatataa gaatggaaaa tattttagaa gtcaaccaaa taaaaaaata
     9241 ctacaaaatt aaaactggat tattacaaaa aactcagtac gttaaagctg ttgatgacgt
     9301 atcgttttca ataaaaaaag gacaaacttt tggattagta ggagaatcgg gttgtggtaa
     9361 gtcaacgtta ggtaaagtga ttatcaggct tgaagatgca acttcaggct caataattgt
     9421 taatggtgaa gatataacaa gattacaagg taaaaaactc agaaaatcac gacaacaata
     9481 tcagatgata tttcaagatc cgtatgcatc attgaatccg atgcaaatgg ttggagatat
     9541 catttcagaa cctattttaa attataaaaa attgccaaaa gaagaaataa aaaaagaagt
     9601 actatattta ttaaaatgtg ttggcctaag tgaagatgca tattataaat atgcacatga
     9661 attttcaggt ggacagagac aaagagtggg aattgcaaga gcattggctt tgcgtccgag
     9721 tttaattgtt gctgatgagc ctgtaagtgc attagatgta tctgttcaat ctcaagtact
     9781 gaatttatta aaagatttac aagaacaatt taacttaagc tatttattta tcgcacatga
     9841 tttaagtgta gtaaaacata taagtgatgt cattggagtt atgtatttag gtcatatagt
     9901 tgaaatcgca tctgataaag aaatttatga aaatcccaaa catccatata caaaagcgtt
     9961 gatttcatca ataccacaaa ttgataaaca taataacaat agaattatat taaaaggaga
    10021 attaccttcg ccaagtaatc cgccgcaagg ttgtcctttt catacaagat gtccgattgc
    10081 aaaagataaa tgtaaagaaa atataccaca attaaaggac attggtgatg aacatcaagt
    10141 tgcttgtttt tatgtaaata aagtaggtga tttaaatggt taaattaata ttaaagagat
    10201 taggtttaat gattccgtta ctaattttaa tttctattgt tgtattttca ttagctatca
    10261 ttcaaccagg agatccattt tcagatttac aaaacggaaa aataaaacaa gaagcgataa
    10321 atgcacaaag ggaaaagtta ggcctcaacg actctatatc acatcaatac attagatggg
    10381 tcaatcatgt tatacatggt gatttagggg agtcaatcaa atataaaagg ccggtaattg
    10441 atgttattga ggaaagaatt ccaaatacaa tattactcgg tgctatgtca ttaattatta
    10501 cttatattat ctcatttgct ttaggaataa cgtcaggtag atattcttac agtttgactg
    10561 attatactgt gcaaatattt aattatttga tgttagctat tccatctttt attgcgggag
    10621 tatttgcaat ttttattttt tcttttgaat tacaatggtt tccgtttcaa ggttctgttg
    10681 atattaacct taaagaaggt acttttgaat attatatgag taaaatttat catacatttt
    10741 tgcctgcatt aactttagga ttattatcta ctgctggtta tattcaatat ttacgtaatg
    10801 atattattga aaattctaaa a
//
LOCUS       DAMZKC010000090        10643 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.091, whole genome shotgun sequence.
ACCESSION   DAMZKC010000090 DAMZKC010000000
VERSION     DAMZKC010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10643
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.091"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            21..1550
                     /gene="aur"
                     /locus_tag="POB55_001698"
     CDS             21..1550
                     /gene="aur"
                     /locus_tag="POB55_001698"
                     /EC_number="3.4.24.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloproteinase aureolysin"
                     /protein_id="HDI6500660.1"
                     /translation="MRKFSRYAFTSMAAVTLLSTLSPAALAIDSKNKPANSDIKFEVT
                     QKSDAVKALKELPKSENVKNIYQDYAVTDVKTDKKGFTHYTLQPSVDGVHAPDKEVKV
                     HADKSGKVVLINGDTDAKKVKPTNKVTLSKDDAADKAFKAVKIDKNKAKNLKDKVIKE
                     NKVEIDGDSNKYVYNVELITVTPEISHWKVKIDAQTGEILEKMNLVKEAAETGKGKGV
                     LGDTKDININSIDGGFSLEDLTHQGKLSAFSFNDQTGQATLITNEDENFVKDEQRAGV
                     DANYYAKQTYDYYKDTFGRESYDNQGSPIVSLTHVNNYGGQDNRNNAAWIGDKMIYGD
                     GDGRTFTSLSGANDVVAHELTHGVTQETANLEYKDQSGALNESFSDVFGYFVDDEDFL
                     MGEDVYTPGKEGDALRSMSNPEQFGQPAHMKDYVFTEKDNGGVHTNSGIPNKAAYNVI
                     QAIGKSKSEQIYYRALTEYLTSNSNFKDCKDALYQAAKDLYDEQTAEQVYEAWNEVGV
                     E"
     gene            1917..2366
                     /locus_tag="POB55_001699"
     CDS             1917..2366
                     /locus_tag="POB55_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HDI6500661.1"
                     /translation="MKKSKRLEIVSTIVKKHKIYKKEQIISYIEEYFGVRYSATTIAK
                     DLKELNIYRVPIDCETWIYKAINNQTEQEMREKFRHYCEHEVLSSIINGSYIIVKTSP
                     GFAQGINYFIDQLNIEEILGTVSGNDTTLILTASNDMAEYVYAKLFK"
     gene            2369..2536
                     /locus_tag="POB55_001700"
     CDS             2369..2536
                     /locus_tag="POB55_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500662.1"
                     /translation="MYQMNNKNLFRITCTQVSYQILTCVRVFLWKERIHSHSIDCIKN
                     FIQHVFMGIFE"
     gene            2713..3948
                     /gene="arcA"
                     /locus_tag="POB55_001701"
     CDS             2713..3948
                     /gene="arcA"
                     /locus_tag="POB55_001701"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HDI6500663.1"
                     /translation="MTDGPIKVNSEIGALKTVLLKRPGKELENLVPDYLDGLLFDDIP
                     YLEVAQKEHDHFAQVLREEGVEVLYLEKLAAESIENPQVRSEFIDDVLAESKKTILGH
                     EEEIKALFATLSNQELVDKIMSGVRKEEINPKCTHLVEYMDDKYPFYLDPMPNLYFTR
                     DPQASIGHGITINRMFWRARRRESIFIQYIVKHHPRFKDANIPIWLDRDCPFNIEGGD
                     ELVLSKDVLAIGVSERTSAQAIEKLARRIFENPQATFKKVVAIEIPTSRTFMHLDTVF
                     TMIDYDKFTMHSAILKAEGNMNIFIIEYDDVNKDIAIKQSSHLKDTLEDVLGIDDIQF
                     IPTGNGDVIDGAREQWNDGSNTLCIRPGVVVTYDRNYVSNDLLRQKGIKVIEISGSEL
                     VRGRGGPRCMSQPLFREDI"
     gene            3981..4991
                     /gene="argF"
                     /locus_tag="POB55_001702"
     CDS             3981..4991
                     /gene="argF"
                     /locus_tag="POB55_001702"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDI6500664.1"
                     /translation="MTEIQKPYDLKGRSLLKESDFTKAEFEGLIDFAITLKEYKKNGI
                     KHHYLSGKNIALLFEKNSTRTRAAFTVASIDLGAHPEFLGKNDIQLGKKESVEDTAKV
                     LGRMFDGIEFRGFSQQAVEDLAKFSGVPVWNGLTDDWHPTQMLADFMTIKENFGYLEG
                     INLTYVGDGRNNIAHSLMVAGAMLGVNVRICTPKSLNPKEAYVDIAKEKASQYGGSVM
                     ITDNIAEAVENTDAIYTDVWVSMGEESEFEQRINLLKDYQVNQQMFDLTGKDSTIFLH
                     CLPAFHDTNTLYGQEIYEKYGLAEMEVTDQIFRSEHSKVFDQAENRMHTIKAVMAATL
                     GS"
     gene            5082..6512
                     /gene="arcD"
                     /locus_tag="POB55_001703"
     CDS             5082..6512
                     /gene="arcD"
                     /locus_tag="POB55_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HDI6500665.1"
                     /translation="MNESGDNKLSKSSLIGLVIGSMIGGGAFNIMSDMGGKAGGLAII
                     IGWIITAIGMISLAFVFQNLTNERPELDGGIYSYAQAGFGDFVGFISAWGYWFSAFLG
                     NVAYATLLMSAVGNFFPIFKGGNTLPSIIVASLLLWGVHFLILKGVETAAFINSIVTV
                     AKLIPILLVIICMIIAFNFDTFKTGFFGMTSEGVLPFSWASTMSQVKSTMLVTVWVFI
                     GIEGAVIFSSRAKNKKDVGSATVIGLISVLIIYFLLTVLAQGVILQNHISQLDSPSMA
                     QVLATIVGGWGSTLVNIGLIISVLGAWLGWTLLAGELPFIVAKDGLFPKWFAKENKNG
                     APVNALLITNILVQLFLISMLFTQSAYQFAFSLASSAILYPYMFSAFYQVKYTLEHRQ
                     QATTKQWTIGIIASIYAIWLIYAAGINYLLLTMLLYIPALLVYSIVQKNNQTRLIKSD
                     YILFMIIIVLAVIGLIKLLMGTINVF"
     gene            6529..7470
                     /gene="arcC"
                     /locus_tag="POB55_001704"
     CDS             6529..7470
                     /gene="arcC"
                     /locus_tag="POB55_001704"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDI6500666.1"
                     /translation="MKEKIVIALGGNAIQTKEATAEAQQTAIRRAMQNLKPLFDSPAR
                     IVISHGNGPQIGSLLIQQAKSNSDTTPAMPLDTCGAMSQGMIGYWLETEINRILTEMN
                     SDRTVGTIVTRVEVDKDDPRFNNPTKPIGPFYTKEEVEELQKEQPDSVFKEDAGRGYR
                     KVVASPLPQSILEHQLIRTLADGKNIVIACGGGGIPVIKKENTYEGVEAVIDKDFASE
                     KLATLIEADTLMILTNVENVFINFNEPNQQQIDDIDVATLKKYAAQGKFAEGSMLPKI
                     EAAIRFVESGENKKVIITNLEQAYEALIGNKGTHIHM"
     gene            7569..8273
                     /locus_tag="POB55_001705"
     CDS             7569..8273
                     /locus_tag="POB55_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDI6500667.1"
                     /translation="MTENFILGRNNKLEHELKALADYINIPYSILQPYQSECFVRHYT
                     KGQVIYFSPQESSNIYFLIEGNIIREHYNQNGDVYRYFNKEQVLFPISNLFHPKEVNE
                     LCTALTDCTVLGLPRELMAFLCKANDDIFLTLFALINDNEQQHMNYNMALTSKFAKDR
                     IIKLFCHLCQTVGYDQDEFYEIKQFLTIQLMSDMAGISRETAGHIIHELKDEKLVVKD
                     HKNWLVSKHLFNDVCV"
     gene            8623..>10643
                     /gene="clfB"
                     /locus_tag="POB55_001706"
     CDS             8623..>10643
                     /gene="clfB"
                     /locus_tag="POB55_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin clumping factor ClfB"
                     /protein_id="HDI6500668.1"
                     /translation="MKKRIDYLSNKQNKYSIRRFTVGTTSVIVGATILFGIGNHQAQA
                     SEQSNDTTQSSKNNASADSEKNNMIETPQLNTTANDTSDISANTNSANVDSTAKPMST
                     QTSNTTTTEPASTNETPQLTAIKDQATAAKMQDQTVPQEANSQVDNKTTNDANSIATN
                     SELKNPQTLDLPQSSPQTISNAQGTSKPSVRTRAVRSLAVAEPVVNAADAKGTNVNDK
                     VTAKDFQLEKTTFDPNQSGNTFMAANFTVTGQVKSGDYFTAKLPDSVTGNGDVDYSNS
                     NNTMPIADIVNDKNEVVAKATYDILTKTYTFVFTDYVNDKQNINGKFSLPLFTDRAKA
                     PKSGTYDANINIADEMFNNKITYNYSSPIAGIDKPNGANISSQIIGVDTASGQNTYKQ
                     TVFVNPKQRVLGNTWVYIKGYQDKIEESSGKVSATDTKLRIFEVNDTSKLSDSYYADP
                     NDSNLKEVTDQFKDKITYKYQNVASINFGDINKTYVVLVEGHYDKTGKNLKTQVIQEN
                     VDPATGKDYSIFGWNNENVVRYGGGSADGDSAVNPKDPTPGPPVDPEPSPDPEPEPSP
                     DPDPEPTPDPEPSPDPDPDSDSDSDSGSDSDSDSDSDSDSDSDSGSDSDSDSDSDSES
                     DSESDSDSDSESDSDSDSDSESDSDSDSDSDSDSDSDSDSDSDSDSDSD"
BASE COUNT         3929 a         1552 c         2059 g         3103 t
ORIGIN      
        1 gatttcagga ggaatgaaat gtgaggaaat tttcaagata tgcatttaca agtatggcag
       61 cagtaacatt gttgagcact ttatcaccag cagcattagc gattgattca aaaaataaac
      121 cagctaattc tgatattaaa tttgaggtga ctcaaaagag tgatgcggtc aaagcattaa
      181 aagaattgcc taaatccgaa aatgtaaaaa atatttatca agattacgct gttactgatg
      241 taaaaactga taaaaaagga tttacgcatt atacattgca accgagtgtt gatggtgttc
      301 atgcacctga caaagaagtg aaagtacacg cagacaaatc aggaaaagtc gttttaatca
      361 atggggatac tgatgcgaag aaagtaaagc caacgaataa agtgacatta agtaaagatg
      421 acgcagccga caaagcattt aaagcagtta agattgataa gaataaagcg aaaaatctta
      481 aagataaagt cattaaagaa aacaaagttg aaatcgatgg tgacagtaat aaatacgttt
      541 ataatgttga gttaattaca gtgacaccag aaatttcaca ttggaaagtt aaaattgatg
      601 ctcaaactgg cgaaatttta gaaaaaatga acttagttaa agaagctgca gaaactggta
      661 aaggaaaagg tgtacttggc gatacaaaag atatcaatat caatagtatt gacggtggat
      721 ttagcctaga agatttaacg catcaaggta aattatcagc atttagcttt aatgatcaaa
      781 caggtcaagc aacattgatt actaatgaag atgaaaactt cgtaaaagat gagcaacgtg
      841 ctggcgtaga tgcaaattat tacgctaaac aaacatatga ttattacaaa gacacatttg
      901 gtcgtgaatc atatgacaac caaggtagtc caattgtttc attaacgcat gttaataact
      961 acggtggtca agataacaga aataatgccg catggattgg tgacaaaatg atctatggtg
     1021 atggtgatgg tcgcacattc acaagtttat cgggtgcaaa tgacgtagta gcacacgaat
     1081 taacacacgg tgtgacacaa gagactgcaa acttagaata taaggaccag tcaggcgctc
     1141 taaatgaaag cttttcagat gtttttggat actttgtaga tgacgaggat ttcttaatgg
     1201 gtgaagatgt ctacacacct ggaaaagagg gagacgcttt acgcagcatg tcaaacccag
     1261 aacaatttgg tcaaccagct catatgaaag actatgtatt cactgaaaaa gataatggtg
     1321 gcgtacatac gaattctgga attccaaata aagcagctta taacgtgatt caagcaatag
     1381 ggaaatctaa atcagaacaa atttactacc gagcattaac ggaatactta acaagtaatt
     1441 caaacttcaa agattgtaaa gatgcattat accaagcggc caaagattta tatgacgagc
     1501 aaacagctga acaggtgtat gaagcatgga atgaagtagg cgtggagtaa aaatatataa
     1561 acaagaagaa gtaatgttaa acacttataa ataattaaat tttaaataca gcttatatat
     1621 ggggtatacc tacattgata attgactttg taggtatacc ctctctttgt aattagatga
     1681 gaggagtgtg agggttgtct gccgaaagac tactcggcag tctaaaatca ttacaagtag
     1741 tagatatgtg ataattaaat gctgacttag aatacaaaat tcattttaaa agttgtcaca
     1801 aaaaatttac atgtattttt attatctttt gcaaaacaaa gtgttaaatt ataaatgaaa
     1861 catgcatgaa tttatttttt aatacaagaa acgtaactac caaaggagtt tacaatatga
     1921 agaaaagtaa acgattagaa attgtttcta caatagttaa aaagcataag atttataaaa
     1981 aagaacaaat catttcatat attgaagaat attttggtgt aagatatagt gcaacaacaa
     2041 ttgctaaaga cttgaaagaa cttaatatat atcgtgtccc tatcgattgt gaaacatgga
     2101 tttataaagc tattaataat caaacagaac aagagatgag agaaaagttt agacactatt
     2161 gtgaacatga agttctaagt tcaatcatca atggttcata cattatcgtt aaaacctcac
     2221 ctggtttcgc ccaaggcata aactatttta tcgatcagct aaatatagaa gagatattag
     2281 gtacggtgag tggaaatgac actacattaa tcttaactgc ctcaaatgat atggcagaat
     2341 acgtatatgc aaaattattt aaatagacat gtatcaaatg aataataaaa atttgtttcg
     2401 tatcacgtgt actcaagtta gttaccaaat attaacttgt gtacgcgttt ttttatggaa
     2461 agaaagaatt catagtcatt caattgactg tataaaaaac tttatacaac atgtttttat
     2521 gggtattttt gaataaaaaa tgtatatttt gacccaaaat acctttattt atgtataaaa
     2581 atccattatt atgtattgta taacaaaaag atatgaaatt ttcgactttc tttatgtgaa
     2641 tataatcaca tgtaagcgtt tgaagattgt ctatactcta aatgaattca aagataaaag
     2701 gaggaaatag acatgacaga tggtccaatt aaagtaaata gcgaaattgg agctttaaaa
     2761 actgtgttac ttaagcgtcc tggaaaagaa ttagaaaatt tagtacctga ttatttagat
     2821 ggattactat ttgatgatat tccatattta gaagtagctc aaaaagagca tgaccatttt
     2881 gcgcaggtgc taagagaaga gggtgttgaa gtactttacc ttgagaagtt agcagctgaa
     2941 agtattgaaa atcctcaagt aagaagtgaa tttattgatg atgtattagc agagtctaaa
     3001 aaaacaatat taggtcatga agaagaaatt aaggcattat ttgcgacact ttctaatcaa
     3061 gaacttgtag ataaaataat gtcaggggta cgtaaggaag aaattaatcc gaaatgtaca
     3121 catctagtag agtatatgga tgataagtat ccattctatt tagatccaat gccaaacctt
     3181 tattttacta gagatccaca agcctcaata ggacacggta taacaatcaa tcggatgttc
     3241 tggagagcac gacgacgaga atcaatattt attcaatata ttgtaaagca tcatcctaga
     3301 tttaaagatg cgaatattcc aatctggtta gatcgagatt gcccattcaa tattgaaggc
     3361 ggcgatgaac ttgttttatc taaagatgtc ttggctatag gcgtttcaga acgtacatct
     3421 gcacaagcta ttgaaaagtt agcgcgacgt atttttgaaa atccgcaggc gacgtttaaa
     3481 aaagtagtag caattgaaat tccaactagt cgaactttta tgcacttaga tacagtattt
     3541 acaatgatag attatgacaa atttacaatg cattcagcca ttttaaaggc agaaggcaat
     3601 atgaatatat ttattattga atatgatgac gtaaataaag atattgccat caaacaatct
     3661 agtcatttaa aagatacttt agaagacgta ctaggtatag atgatatcca attcattcca
     3721 acaggaaatg gtgatgtcat tgatggtgct agagagcaat ggaatgatgg ctcaaataca
     3781 ttatgtataa gaccaggcgt tgtagtgact tacgatagaa actatgtatc gaatgattta
     3841 ttgagacaaa aaggcattaa agtcattgaa atatctggta gcgagttggt acgtggacgt
     3901 gggggcccta gatgtatgag tcaaccatta ttcagagaag acatttaacg taataaatta
     3961 tagaaacgag ggtgaaaata atgacagaaa ttcaaaaacc gtatgattta aaaggcagat
     4021 cattattaaa agaaagtgat tttaccaaag cagaattcga aggacttatt gattttgcaa
     4081 ttacattaaa agagtataag aaaaacggta ttaagcatca ctacttatct ggaaaaaata
     4141 ttgcactact attcgaaaag aattcgacga gaacgcgtgc tgcgtttaca gttgcgtcta
     4201 ttgatttagg tgcgcatcca gaatttttag ggaaaaatga tattcaatta ggcaaaaaag
     4261 aatctgtaga ggatactgcg aaagtattag gtagaatgtt cgatggtatt gaattccgtg
     4321 gtttttcaca acaagctgtt gaagatttag cgaagttctc tggtgtgccg gtgtggaatg
     4381 gattaacaga cgattggcat cctacacaaa tgttagctga ttttatgaca ataaaagaga
     4441 attttggata tctagaagga ataaacttaa cttacgttgg agatggacgt aataatattg
     4501 cgcattcatt aatggtagca ggtgctatgt taggtgttaa tgtaagaatt tgtacaccta
     4561 aatcattaaa tccaaaagag gcatatgttg atattgcaaa agaaaaagcg agtcaatatg
     4621 gtggttcagt catgattacg gataatattg cagaagcagt tgaaaataca gatgctatat
     4681 atacagatgt ttgggtatcg atgggtgaag aaagtgaatt tgaacaacgt attaatttat
     4741 taaaagacta tcaagtgaat caacagatgt ttgatttaac aggtaaagat tcaacgatat
     4801 tcttacattg tttaccagca ttccatgata caaatacact ttatggacaa gaaatttatg
     4861 aaaaatatgg attagctgaa atggaagtta cagaccaaat ctttagaagt gaacattcaa
     4921 aagtgtttga tcaagctgaa aatagaatgc atacaattaa ggcagtaatg gcagcaacat
     4981 tggggagtta atcactaaat ggaacgatat gaatatgatg tgtctgatga tataagtgtc
     5041 atgtacagac acctcatatt ggtattaaag gagaaatgaa tatgaacgaa tcaggagata
     5101 acaaactcag taaatcttct ttaattggac tagttatagg atccatgatt ggtggcggtg
     5161 cgttcaatat aatgtctgat atgggcggta aagccggtgg attagccatt attattggtt
     5221 ggattattac agctatagga atgatttcat tagcgttcgt atttcaaaat ttaaccaatg
     5281 aacggccgga gctagacggt ggtatttata gttatgctca agcaggattt ggcgattttg
     5341 taggatttat cagtgcttgg ggatattggt tctcagcgtt tttaggcaat gttgcctatg
     5401 caacactatt gatgtcagca gtaggtaact ttttcccgat ttttaaagga ggcaacacat
     5461 taccaagtat tattgtcgcc tcgttactac tctggggtgt ccatttcttg attttaaaag
     5521 gcgttgaaac agcagcattt atcaatagta ttgttactgt tgcaaagtta ataccgattt
     5581 tacttgtaat catatgcatg ataattgcat tcaattttga cacttttaaa acaggctttt
     5641 tcggtatgac gtcagagggt gtattgccat ttagttgggc gagcacaatg agccaagtta
     5701 aaagtacgat gctagtgaca gtttgggtgt ttatcggtat cgagggtgca gtaatttttt
     5761 ctagtagagc taaaaataaa aaagatgtag gtagtgccac ggttatagga cttatatcag
     5821 ttttaattat ctatttctta ttaactgtat tagctcaagg cgtgattttg caaaatcata
     5881 tttctcaatt agattcgcca agtatggcac aggtgcttgc aactattgta ggtggttggg
     5941 gatctacact tgtaaatatt ggtttaatta tttcggtact aggtgcatgg ttaggatgga
     6001 cattgcttgc tggtgaatta cctttcattg ttgcaaaaga tggattattt ccaaagtggt
     6061 ttgctaaaga aaataaaaat ggagcacctg taaatgcact gcttattacc aatatattag
     6121 tacaattatt tttaataagt atgctattta cacagagtgc gtatcaattt gcattttcac
     6181 tagcatcaag tgctatttta tacccttaca tgttcagtgc attttaccaa gttaaataca
     6241 ctttagagca tcgacagcaa gcaactacta agcaatggac gattggcatc atagcctcaa
     6301 tttatgctat atggcttata tatgcagcag gtatcaatta cttattattg acgatgttac
     6361 tttatattcc agctcttctt gtttattcaa tcgttcaaaa gaataatcag acacgtttga
     6421 ttaaatcaga ctatattctt tttatgatta ttatcgtact tgcagttatc ggattaatta
     6481 agttattgat gggaacgata aatgtttttt aaaaggagcg acaaaaatat gaaagagaaa
     6541 attgtcattg cattaggcgg taatgcgata cagacaaaag aagcaacagc tgaagcacaa
     6601 caaacagcta ttagacgtgc gatgcaaaac cttaaacctt tatttgattc accagcgcgt
     6661 attgtcattt cacatggtaa tggcccacaa attggaagtt tattaatcca acaagctaaa
     6721 tcgaacagtg acacaacgcc ggcaatgcca ttggatactt gtggtgcaat gtcacagggt
     6781 atgataggct attggttgga aactgaaatc aatcgcattt taactgaaat gaatagtgat
     6841 agaactgtag gcacaatcgt tacacgtgtg gaagtagata aagatgatcc acgattcaat
     6901 aacccaacca aaccaattgg tcctttttat acgaaagaag aagttgaaga attacaaaaa
     6961 gaacagccag actcagtctt taaagaagat gcaggacgtg gttatagaaa agtagttgcg
     7021 tcaccactac ctcaatctat actagaacac cagttaattc gaactttagc agacggtaaa
     7081 aatattgtca ttgcatgcgg tggtggcggt attccagtta taaaaaaaga aaatacctat
     7141 gaaggtgttg aagcggttat agataaagat tttgctagtg agaaattagc aacgctgatt
     7201 gaagcagata ccttaatgat tcttacgaat gtagaaaatg tatttattaa ctttaatgaa
     7261 cctaatcaac aacaaatcga tgatattgat gtagcaacac tgaaaaaata cgcggcacaa
     7321 ggtaagtttg cggaaggatc gatgttgcca aaaatagaag ctgcgatacg atttgttgaa
     7381 agtggggaaa acaaaaaagt tatcattacc aatttagagc aggcatacga agctttgatt
     7441 ggtaataaag gtacacacat tcacatgtag cactttatca cgcgacaaaa cattaaatat
     7501 gtttctccgt tgattcaaat gaaaaagttg tctgctgaca ctttgcaagg tttgaaggag
     7561 tttaacttat gacagaaaac tttattttgg gtagaaataa taaattagaa catgaactaa
     7621 aggcattagc agattacatt aatatcccat atagtatatt acaaccatat caaagtgaat
     7681 gttttgtcag acattatacg aaaggccaag ttatttattt ttcgccacaa gaaagtagca
     7741 atatttactt tttaattgaa ggtaacatta ttagagaaca ttacaatcaa aatggagatg
     7801 tatatcgtta ttttaataaa gagcaagtat tatttccaat cagtaactta tttcatccga
     7861 aagaggttaa cgaattgtgt acagcattaa ccgattgtac agttcttgga ttgcctagag
     7921 aattgatggc ctttttgtgc aaagctaatg atgatatatt tttgacactt tttgcattaa
     7981 taaatgataa tgagcagcaa cacatgaact ataacatggc attaacaagt aaatttgcta
     8041 aagatcgaat tattaaattg ttttgtcatc tatgtcagac agtagggtac gatcaagatg
     8101 aattttatga aatcaaacag tttttaacta ttcaactcat gagtgatatg gctgggattt
     8161 cccgggaaac agctggtcat attattcatg aacttaaaga tgaaaaactt gttgttaaag
     8221 atcataaaaa ttggttagta agcaaacatt tattcaatga tgtatgtgtt taatatacaa
     8281 tgtaaaatga ataagttgaa catgaggtct aacgtacatt tatacgttag gccttttttg
     8341 ctagcatgat gaataattta aaatgttagt taaatttgat tgttgaaatt acagtaaaat
     8401 ttaaggtgat gaaaaattta gaacttctaa gtttttgaaa agtaaaacat ttgcaatagt
     8461 gtaaaaatgg tatattgatt tttgctagtt aacagaaaat tttaagttat ataaatagga
     8521 agaaaacaaa ttttacgtaa tttttttcga aaagcaattg atataattct tatttcatta
     8581 tacaatttag actaatctag aaattgaaat ggagtaatat ttttgaaaaa aagaattgat
     8641 tatttgtcga ataagcagaa taagtattcg attagacgtt ttacagtagg taccacatca
     8701 gtaatagtag gggcaactat actatttggg ataggcaatc atcaagcaca agcttcagaa
     8761 caatcgaacg atacaacgca atcttcgaaa aataatgcaa gtgcagattc cgaaaaaaac
     8821 aatatgatag aaacacctca attaaataca acggctaatg atacatctga tattagtgca
     8881 aacacaaaca gtgcgaatgt agatagcaca gcaaaaccaa tgtctacaca aacgagcaat
     8941 accactacaa cagagccagc ttcaacaaat gaaacacctc aactgacggc aattaaagat
     9001 caagcaactg ctgcaaaaat gcaagatcaa actgttcctc aagaagcaaa ttctcaagta
     9061 gataataaaa caacgaatga tgctaatagc atagcgacaa acagtgagct taaaaatcct
     9121 caaacattag atttaccaca atcatcacca caaacaattt ccaatgcgca aggaactagt
     9181 aaaccaagtg ttagaacgag agctgtacgt agtcttgcag ttgctgaacc tgtagtaaat
     9241 gctgctgatg ctaaaggtac aaatgtaaat gataaagtta cggcaaaaga ttttcaatta
     9301 gaaaagacta catttgaccc taaccaaagt ggtaatactt ttatggcggc aaactttaca
     9361 gtgactggac aagtgaaatc aggggattat tttacagcga agttaccaga tagtgtaact
     9421 ggtaatggag acgtggatta ctctaattcg aataatacga tgccaattgc agatatagta
     9481 aacgataaaa atgaagttgt agcaaaagcg acatatgata ttttgactaa gacatataca
     9541 tttgtcttta cagattatgt aaatgataag caaaatatta atgggaaatt ttcattacca
     9601 ctatttacag acagagcaaa ggcacctaaa tcaggaacat atgatgcaaa tattaatatt
     9661 gcggatgaaa tgtttaataa taaaattact tataactata gttcgccaat tgcaggaatt
     9721 gataagccaa atggcgcgaa catttcttct caaattattg gtgtagatac agcttcaggt
     9781 caaaatacat acaagcaaac ggtatttgtt aaccctaagc aacgagtttt aggtaatacg
     9841 tgggtgtata ttaaaggtta ccaagataaa atcgaagaaa gtagcggtaa agtaagtgct
     9901 acagatacaa aactgagaat ttttgaagtg aatgatacat ctaaattatc agatagctac
     9961 tatgcagacc caaatgactc taatcttaaa gaagtaactg atcaatttaa ggataaaatc
    10021 acttataaat accaaaatgt agcaagtatt aattttggtg atataaataa aacgtatgtc
    10081 gttttagtcg aaggtcatta tgataaaaca ggtaaaaact tgaaaacgca agtcattcaa
    10141 gaaaatgttg acccagcgac aggtaaagac tacagtattt tcggttggaa taatgagaat
    10201 gttgtacgtt atggtggtgg aagtgctgat ggtgattcag cagtaaatcc gaaagaccca
    10261 actccagggc caccggttga cccagaacca agtccagacc cagaaccgga accaagtcca
    10321 gacccggatc cggaaccaac gccagatcca gaaccaagcc cagacccgga tccggattcg
    10381 gattcagaca gcgactcagg ttcagatagc gattcggatt cagacagcga ttcagactca
    10441 gacagcgact caggttcaga tagcgattcg gattcagaca gcgactcaga atcagacagt
    10501 gagtcagatt cagatagcga ttcagaatca gatagcgact cagattcaga tagtgagtca
    10561 gattcagaca gtgactcgga ctcagatagc gattcagact cagacagcga ttcagactca
    10621 gacagcgact cagattcaga tag
//
LOCUS       DAMZKC010000091        10639 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.092, whole genome shotgun sequence.
ACCESSION   DAMZKC010000091 DAMZKC010000000
VERSION     DAMZKC010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10639
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.092"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            54..1007
                     /locus_tag="POB55_001707"
     CDS             54..1007
                     /locus_tag="POB55_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500669.1"
                     /translation="MKKTLGCLLLIMLLVVAGCSFGGNHKLSSKKSEESKQETVKKES
                     EEEKDPDLEKYEEIEKKMKGIKDAPSLDKLDPLMTEKSFTNSKGIQGWKDYKELMGKV
                     ELADYRFTKDSKGSSIKDVDAFFKGKKGIKRKVIETHDDVKQVDYWYVDPDGKKIGNS
                     NTPVFYAEIMTKYKDGKLVYASVEPGSYVIHKDDAIKYDDYSKLKKLSQLTKLDHPKP
                     VPYSVAQIKSFGVPLTSVSFMTHGSKDTKDEVLPALAYFTFSPKNYEDKSNPDPKVLN
                     LVHMDFLNASSDFGNAHFVVLSKYIKEYESNYETASDDSLK"
     gene            complement(1170..1373)
                     /locus_tag="POB55_001708"
     CDS             complement(1170..1373)
                     /locus_tag="POB55_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HDI6500670.1"
                     /translation="MQVNKVIYILLALFLGSFGIHKFYAGKNMQGILHLIFCWTGIPH
                     ILAIISAVITVFKPADEQGNVTL"
     gene            1672..2160
                     /locus_tag="POB55_001709"
     CDS             1672..2160
                     /locus_tag="POB55_001709"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDI6500671.1"
                     /translation="MAIKKLVPASHPILTKKAQAVIKFDDSLKRLLQDLEDTMYAQEA
                     AGLCAPQINQSLQVAIIDMEMEGLLQLVNPKIISQSNETITDLEGSITLPDVYGEVTR
                     SKMIVVESYDVNGNKVELTAHEDVARMILHIIDQMNGIPFTERADRILTDKEVEAYFI
                     ND"
     gene            2153..3088
                     /locus_tag="POB55_001710"
     CDS             2153..3088
                     /locus_tag="POB55_001710"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HDI6500672.1"
                     /translation="MTKIIFMGTPDFSTTVLEMLIAEHDVIAVVTQPDRPVGRKRVMT
                     PPPVKKVAMKYDLPVYQPEKLSGSEELEQLLQLDVDLIVTAAFGQLLPESLLALPKLG
                     AINVHASLLPKYRGGAPIHQAIIDGEQETGITIMYMVKKLDAGNIISQQAIKIEENDN
                     VGTMHDKLSVLGADLLKETLPSIIEGTNESVPQDDTQATFASNIRREDERINWNKPGR
                     QVFNQIRGLSPWPVAYTTMDDTNLKIYDAELVETNKINEPGTIIETTKKAIIVATNDN
                     EAVAIKDMQLAGKKRMLAANYLSGAQNTLVGKKLI"
     gene            3085..4392
                     /gene="rsmB"
                     /locus_tag="POB55_001711"
     CDS             3085..4392
                     /gene="rsmB"
                     /locus_tag="POB55_001711"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDI6500673.1"
                     /translation="MIENVRSLAFDTIQDILNEGAYSNLRINEVLSENELNAMDKALF
                     TEIVYGTVKRKFTLDFYLKPFVKTKIKAWVRQLLWMSIYQYVYLDKVPNHAIINEAVE
                     IAKERGGYHNGNVVNGILRTMMRSDLPDFNEIADPKKRMAIEYSMPKWIIDHWATHYG
                     LEKTETILQSFLETTSTTVRANLTRASLDDIIEKLQDEGYDVEKDHDLPYCLHIGGQP
                     IIHSRSFKDGFVSIQDKSSMFVAHIMNVDRHDHVLDACSAPGGKACHIAEVLMPEGQV
                     DASDIHDHKIDLINFNIKKLRLTNIKAFQHDATKPYDKTYDKILVDAPCSGLGVMRHK
                     PEIKYTQSKQHIESLVELQLEILENVKNNVKIGGEIIYSTCTIEQLENENVIYTFLKN
                     NKNFEFEPFQHPITGELVKTLQIMPQDFNSDGFFITKIKRKDN"
     gene            4395..5489
                     /gene="rlmN"
                     /locus_tag="POB55_001712"
     CDS             4395..5489
                     /gene="rlmN"
                     /locus_tag="POB55_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002435140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HDI6500674.1"
                     /translation="MITAEKKKKNKFLPNFDKQSIYSLRFDEMQNWLVEQGQQKFRAK
                     QIFEWLYQKRVDSIDEMTNLSKDLRQLLKDNFTVTTLTTVVKQESKDGTIKFLYELQD
                     GYTIETVLMRHDYGNSVCVTTQVGCRIGCTFCASTLGGLKRNLEAGEIVSQVLTVQKA
                     LDATEERVSQIVIMGIGEPFENYDEMMDFLRIVNDDNSLNIGARHITVSTSGIIPRIY
                     DFADEDIQINFAVSLHAAKDEVRSRLMPINRAYNVEKLIEAIQYYQEKTNRRVTFEYG
                     LFGGVNDQLEHARELAHLIKGLNCHVNLIPVNHVPERNYVKTAKNDIFKFEKELKRLG
                     INATIRREQGSDIDAACGQLRAKERQVETR"
     gene            5496..6239
                     /locus_tag="POB55_001713"
     CDS             5496..6239
                     /locus_tag="POB55_001713"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase Stp1"
                     /protein_id="HDI6500675.1"
                     /translation="MLEAQFFTDTGQHRDKNEDAGGIFYNRTNQQLLVLCDGMGGHKA
                     GEVASKFVTDELKSRFEAENLIEEHQAENWLRNNIKDINFQLYHYAQENAEYKGMGTT
                     CVCALVFEKSVVIANVGDSRAYVINSRQIEQITSDHSFVNHLVLTGQITPEEAFTHPQ
                     RNIITKVMGTDKRVSPDLFIKRLNFYDYLLLNSDGLTDYVKDNEIKRLLVKEGTIEDH
                     GDQLMQLALDNHSKDNVTFILAAIEGDKV"
     gene            6236..8230
                     /gene="pknB"
                     /locus_tag="POB55_001714"
     CDS             6236..8230
                     /gene="pknB"
                     /locus_tag="POB55_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase Stk1"
                     /protein_id="HDI6500676.1"
                     /translation="MIGKIINERYKIVDKLGGGGMSTVYLAEDTILNIKVAIKAIFIP
                     PREKEETLKRFEREVHNSSQLSHQNIVSMIDVDEEDDCYYLVMEYIEGPTLSEYIESH
                     GPLSVDTAINFTNQILDGIKHAHDMRIVHRDIKPQNILIDSNKTLKIFDFGIAKALSE
                     TSLTQTNHVLGTVQYFSPEQAKGEATDECTDIYSIGIVLYEMLVGEPPFNGETAVSIA
                     IKHIQDSVPNVTTDVRKDIPQSLSNVILRATEKDKANRYKTIQEMKDDLSSVLHENRA
                     NEDVYELDKMKTIAVPLKKEDLAKHISEHKSNQPKRETTQVPIVNGPAHHQQFQKPEG
                     TVYEPKPKKKSTRKIVLLSLIFSLLMIALVSFVAMAMFGNKYEETPDVIGKSVKEAEQ
                     IFNKNNLKLGKISRSYSDKYPENEIIKTTPNTGERVERGDSVDVVISKGPEKVKMPNV
                     IGLPKEEALQKLKSLGLKDVTIEKVYNNQAPKGYIANQSVTANTEIAIHDSNIKLYES
                     LGIKQVYVEDFEHKSFSKAKKALEEKGFKVESKEEYSDDIDEGDVISQSPKGKSVDEG
                     STISFVVSKGKKSDSSDVKTTTESVDVPYTGKNDKSQKVKVYIKDKDNDGSTEKGSFD
                     ITSDQRIDIPLRIEKGKTASYIVKVDGKTVAEKEVSYDDV"
     gene            8458..9333
                     /gene="rsgA"
                     /locus_tag="POB55_001715"
     CDS             8458..9333
                     /gene="rsgA"
                     /locus_tag="POB55_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HDI6500677.1"
                     /translation="MKTGRIVKSISGVYQVDVNGERFNTKPRGLFRKKKFSPVVGDIV
                     EFEVQNINEGYIHQVFERKNELKRPPVSNIDTLVIVMSAVEPNFSTQLLDRFLVIAHS
                     YQLNARVLVTKKDKTPIEKQFEINELLKIYENIGYETEFIGNDDDRKKIVEAWPAGLI
                     VLSGQSGVGKSTFLNHYRPELNLETNDISKSLNRGKHTTRHVELFERQNGYIADTPGF
                     SALDFDHIDKDEIKDYFLELNRYGETCKFRNCNHIKEPNCNVKHQLEIGNIAQFRYDH
                     YLQLFNEISNRKVRY"
     gene            9334..9978
                     /locus_tag="POB55_001716"
     CDS             9334..9978
                     /locus_tag="POB55_001716"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDI6500678.1"
                     /translation="MTKLYPSLLSVDFLDLQHELKRLEEAGVDGVHFDVMDGQFVPNI
                     SIGLPILDAVRKGTTLPIDVHLMIENPEKYIASFAEHGADMISIHVESTPHIHRAIQM
                     IKHLDKKAGVVINPGTPISQIEPILDIVDYVLVMTVNPGFGGQSFIDQCVEKIAGLNA
                     IKMERQLNFDIEVDGGVNTDTAKVCVENGATMLVTGSFFFKQEDYKKVTQALKG"
     gene            9985..10626
                     /locus_tag="POB55_001717"
     CDS             9985..10626
                     /locus_tag="POB55_001717"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HDI6500679.1"
                     /translation="MHINLLCSDRHLPQDIWAKSNEGKWGGVDRGALILLKHQIIPFF
                     SVGDFDSVSKEERQLLTEQLQIKPVQAEKADTDLALAVDKAVALGFDSITIYGATGGR
                     LDHFFGAIQLLLKKAYYKHDVHIEVIDQQNKIELLPKGQHTVEKDKSYPYISFIPMTD
                     DVELSLAGFKYNLARQMLNIGSTLTISNEIESLQAKVTVHDGLILQIRSTDLN"
BASE COUNT         3973 a         1463 c         2005 g         3198 t
ORIGIN      
        1 aatttatgta tgtttatttg attcaaacaa aataacttaa gaggagaaag tttatgaaaa
       61 agacactggg atgtttactt ttaattatgc ttttagtcgt agcaggttgt tcttttggtg
      121 ggaatcataa attatcatca aagaaatcag aagaatcaaa acaagaaact gtaaaaaaag
      181 aatcggaaga agagaaagat ccagatttag agaaatatga agaaatagag aagaaaatga
      241 aaggaattaa agatgcgcca tctcttgata agttggatcc attaatgaca gaaaagtcgt
      301 ttacgaatag taaagggatt caaggatgga aagattacaa agaattaatg ggtaaagtgg
      361 aacttgcaga ttatagattt actaaagatt caaaaggatc ttcaataaaa gatgttgatg
      421 cattctttaa aggtaagaaa ggtataaaaa ggaaagtgat tgaaacacac gatgatgtaa
      481 aacaagttga ttattggtat gtagatccag atggaaagaa aattggcaat tcaaacacac
      541 ctgtttttta cgcagaaatt atgacaaaat ataaagatgg aaagttagtt tatgcatcag
      601 tcgaaccagg atcttacgta atacataaag atgatgcaat taaatatgac gattattcta
      661 agttaaaaaa attaagtcag ctaactaaac ttgatcatcc aaaaccagtt ccatatagcg
      721 tagctcaaat caaatctttc ggagtacctt taacaagcgt ttcatttatg acacatggat
      781 caaaggatac taaagatgaa gtgttgccgg cattggccta tttcactttt tcaccaaaaa
      841 attatgaaga caagtctaat ccagatccaa aagttttaaa tttagtacat atggatttct
      901 taaatgcatc tagtgatttt ggtaacgcac attttgttgt tttaagtaaa tatattaaag
      961 agtatgaatc aaactatgaa acagcgtcag atgattcttt aaaatagtat ttactgtgtg
     1021 aaaaataaat agtgtactac attaaataat cgcaataata atcccgataa acaatcagca
     1081 ttactgctta tcatatagag ttcgtaataa ctataactct atgattcgca aataataaat
     1141 gattgtcatc gggatttatt tttatcaatt tataaagtga cattaccttg ttcatcagca
     1201 ggtttgaaaa cagtaatcac tgcactaata attgctaaaa tgtgtgggat acctgtccaa
     1261 cagaatatta agtgtagaat accttgcata ttcttgccgg cataaaattt atgaatacca
     1321 aaactaccta agaacaatgc taataaaata taaataactt tgtttacttg catttctttc
     1381 cctccagttg aattgcttat aatgacatta gcttctcttt ttattatacc cacttttagt
     1441 tcaaacattc tagtttaagc attcccaatc atctaaattt cagttattca atccttacaa
     1501 taaatttagg attacacttc agttgcattg tattatttta cgtgtgaaat atacgtaatg
     1561 aatcacatga caatctttaa attgaaaaat atacattcta tgatgtaagg tcgcattttt
     1621 aatatattta cgttataatg gtttgatgaa ttttattgag gagtaagagc tatggcgatt
     1681 aaaaagttag taccagcatc gcatcctatt ttaacgaaaa aagcgcaagc agttataaaa
     1741 tttgatgatt cgttaaaaag attattacaa gatttagaag atacaatgta tgcacaagaa
     1801 gcagctggct tatgtgcacc tcaaattaat cagtcattgc aagtggcaat cattgatatg
     1861 gaaatggaag gattattaca acttgttaat ccgaaaatta ttagtcaatc aaatgaaacg
     1921 ataacagact tagaaggttc aattacattg ccagatgttt acggcgaagt gacaagaagt
     1981 aaaatgatag ttgtcgaaag ttatgacgtc aatgggaaca aagttgaact aactgcacat
     2041 gaagatgtag caagaatgat tttgcatatt atagatcaaa tgaacggtat cccttttaca
     2101 gaacgtgcgg accgtatttt aacagataaa gaagtggagg catattttat aaatgactaa
     2161 aataatattt atgggtacac cagacttttc aacaactgtt ttagaaatgc ttattgcaga
     2221 acatgatgtc attgcagtcg taacgcaacc agatcgacct gttggacgta aacgtgttat
     2281 gacaccacca ccagttaaaa aagttgcaat gaaatatgat ttacctgtat atcagcctga
     2341 aaaattaagt ggatcagaag aattagaaca attgcttcaa ttagatgtag atttaattgt
     2401 aactgctgct tttggacaat tattacctga atcattgttg gcattaccaa aacttggggc
     2461 aattaatgta catgcatcat tgttaccgaa gtatagaggt ggtgcaccaa ttcatcaggc
     2521 aattatcgat ggtgaacaag aaaccggcat aacaattatg tatatggtta aaaaattaga
     2581 tgcgggtaat attatttcgc aacaagcaat taaaatagaa gaaaatgata atgtcggtac
     2641 gatgcatgat aaattaagtg tattaggggc agatttatta aaagaaactt taccatctat
     2701 tatagagggc acaaatgaaa gtgtacctca agatgatacg caagcaacat ttgcttccaa
     2761 tattcgacgc gaagatgagc gaattaactg gaataaacca ggaagacaag tgtttaatca
     2821 aattcgtgga ttatcaccat ggccagttgc ttatacaact atggatgaca ctaacttgaa
     2881 aatatacgat gctgaactcg ttgagactaa taagataaac gagcctggaa ccattataga
     2941 aacgactaaa aaagccatta ttgttgctac aaatgataat gaagctgttg caattaaaga
     3001 tatgcaatta gctgggaaaa agagaatgtt agctgccaat tatttaagtg gtgcgcaaaa
     3061 cacactagta gggaagaaac ttatatgata gaaaacgtga gaagtcttgc ttttgacacg
     3121 attcaagata tattaaatga aggtgcgtat agtaacttgc gtatcaatga agtgttgtca
     3181 gaaaatgaat taaatgcaat ggataaggct ttatttacag aaattgtcta cggaaccgtt
     3241 aaaagaaaat ttacgttaga tttttattta aagccttttg tgaaaacaaa aattaaggca
     3301 tgggttaggc aattattatg gatgagtatt tatcaatatg tttatttaga taaagttcca
     3361 aatcacgcca tcattaatga agcagttgaa atagcaaaag aacgcggtgg ctatcataat
     3421 ggtaatgtcg taaatggtat tttacgtaca atgatgcgta gtgacttacc tgattttaat
     3481 gaaattgcag atcctaaaaa aagaatggca atcgagtata gtatgccgaa gtggattata
     3541 gatcattggg caacacatta tggtctcgaa aaaactgaaa caattttaca gtcattttta
     3601 gaaacgacat caacaactgt gcgtgccaac ctgacgcgag catcattaga tgatattatt
     3661 gaaaagttgc aagacgaagg ttatgacgtt gaaaaagatc atgacttacc ttattgtctc
     3721 catataggag gacaaccaat tattcattct cgttcattta aagatggatt cgtttcaatt
     3781 caagataaaa gctcaatgtt tgttgcacac attatgaatg tagaccgaca tgatcacgta
     3841 ttagatgcat gtagtgcacc tggcggtaaa gcttgtcaca ttgctgaagt tttaatgcca
     3901 gaagggcaag ttgacgcttc agatatacat gatcacaaaa tagacttaat taattttaat
     3961 ataaaaaaat tacgattaac aaatattaaa gcttttcaac atgatgcgac aaaaccttat
     4021 gataaaacat acgataagat acttgttgat gcaccatgta gcggattagg tgtaatgaga
     4081 cataagccgg agattaagta tactcaaagc aaacaacata ttgagtcact agttgaatta
     4141 cagcttgaaa tattggaaaa tgtaaaaaac aatgtaaaaa taggtggcga aatcatctat
     4201 tcaacatgta caattgagca actagaaaat gaaaacgtga tttatacgtt tttgaaaaat
     4261 aataaaaact tcgaatttga accgtttcaa catccgataa ctggagagtt ggtcaaaacg
     4321 ttacaaatca tgccgcaaga ctttaattca gatggattct ttatcactaa gataaaaaga
     4381 aaggacaatt aggaatgata actgctgaaa agaaaaagaa gaataaattt cttccaaatt
     4441 ttgacaagca atcaatatat tcattgcgat ttgacgaaat gcaaaactgg ctcgttgaac
     4501 aaggtcaaca aaaatttcga gcgaaacaga tttttgaatg gttatatcaa aaaagagtag
     4561 attcgattga tgaaatgacg aacttatcga aagacttacg acagctttta aaagataact
     4621 ttactgttac aactttaaca actgtagtaa aacaagaaag taaagacggt acaattaaat
     4681 tcttatatga attacaagat ggctatacaa ttgaaactgt tttaatgaga catgattatg
     4741 gaaattcagt atgtgtaacg acacaagtag gttgtcgtat cggatgtacg ttttgtgctt
     4801 ctacacttgg cggcttaaaa agaaaccttg aagctggcga aattgtttca caagttttaa
     4861 cagttcaaaa agcccttgat gctacagaag agcgcgtatc tcaaattgtc ataatgggta
     4921 tcggtgaacc atttgaaaat tatgatgaaa tgatggactt tttaagaatc gtcaatgatg
     4981 ataatagttt aaatattggt gcacgtcaca ttacagtatc aacatcaggt atcattccta
     5041 gaatatacga ctttgcggat gaagatatcc aaattaattt tgctgtaagc ttacacgccg
     5101 caaaagatga agtgcgatca cgcttgatgc caattaaccg tgcatataat gttgagaagt
     5161 taatcgaagc aattcaatat tatcaagaaa aaacaaatcg tcgtgttact tttgaatatg
     5221 gtctgtttgg tggtgtgaat gaccaactag aacatgcaag agaattagca catttaataa
     5281 aaggcttaaa ctgccatgtt aacttaattc ctgtcaacca tgttccagaa agaaattatg
     5341 tgaaaacggc taaaaatgat atctttaaat ttgaaaaaga attaaagaga ctaggaatta
     5401 atgccacaat acgtcgtgaa caaggttcgg atattgacgc agcttgtggt caattaagag
     5461 caaaggaacg acaagtagaa acgaggtaaa gacaaatgct agaggcacaa ttttttactg
     5521 atactggaca acatagagat aagaatgaag atgcgggtgg tattttttat aatcgaacta
     5581 atcaacaact tttagttctg tgtgatggta tgggcggcca taaagcagga gaagttgcaa
     5641 gtaaatttgt tacagatgag ttgaaatccc gttttgaagc ggaaaatctt atagaagaac
     5701 atcaagctga aaattggttg cgtaataata taaaagatat aaattttcag ttatatcact
     5761 atgcacaaga aaatgcagaa tataaaggta tgggtacaac atgtgtttgt gcacttgttt
     5821 ttgaaaaatc agttgtgata gcaaatgtcg gtgattctag agcctatgtt attaatagta
     5881 gacaaattga acaaattact agtgatcact catttgttaa tcatcttgtt ttaacgggtc
     5941 aaattacgcc ggaagaagca tttacacatc cacaacgtaa tattattacg aaggtgatgg
     6001 gcacagataa acgtgtgagt ccagatttgt ttattaagcg attaaatttt tatgattatt
     6061 tattattaaa ttcagatgga ttaactgatt atgttaaaga caatgaaatt aagcgtttgt
     6121 tagtaaaaga aggtacaata gaagatcatg gtgatcaatt aatgcaattg gcattagata
     6181 accattcgaa agataacgtt actttcatac tcgcggctat tgaaggtgat aaagtatgat
     6241 aggtaaaata ataaatgaac gatataaaat tgtagataag cttggcggcg gtggcatgag
     6301 taccgtttat cttgctgaag atacgatact taacattaaa gttgcaatta aggcgatttt
     6361 tataccacct agagaaaaag aagaaacatt aaaacgtttt gaacgagaag tacataactc
     6421 atcacagcta tcacatcaaa atatagtaag tatgatcgat gttgatgaag aagatgactg
     6481 ttactactta gtaatggaat atattgaagg tccgactttg tctgagtata ttgaaagtca
     6541 tgggccatta agtgttgaca cggcgattaa ttttacgaat caaatattgg atggtattaa
     6601 acatgcgcat gatatgcgta ttgtacatag agatattaag ccacaaaata tattaattga
     6661 cagcaataaa acgttgaaaa tatttgattt tggaattgct aaagctttaa gtgagacgtc
     6721 tttaactcag actaatcatg tgttaggtac tgtgcagtac ttttcgccag aacaagcaaa
     6781 aggtgaggca acggatgaat gtacagatat ttattctata ggtattgtgt tatatgaaat
     6841 gcttgttggt gaaccaccct ttaatggaga aactgcagtt agcattgcga ttaaacatat
     6901 tcaggattct gtgccaaatg tgacaacaga tgtacgtaag gatattccgc aatctttaag
     6961 taatgtcatt ttacgcgcta cagaaaaaga caaagcgaat cgttacaaaa caattcaaga
     7021 aatgaaagat gatttgagta gtgttttaca tgaaaatcga gcgaatgaag atgtctatga
     7081 actcgataaa atgaaaacga tagcggtacc tttgaaaaaa gaagatctag caaagcatat
     7141 tagtgaacat aagtcgaatc aacctaaacg tgaaacgacg caagtaccta ttgtaaatgg
     7201 gcctgctcat catcagcaat tccaaaagcc agaaggtacg gtttacgaac caaaacctaa
     7261 aaagaaatca acacgaaaga ttgtgctctt atcactaatc ttttcgttgt taatgattgc
     7321 acttgtttct tttgtggcaa tggcaatgtt tggtaataaa tacgaagaga cacctgatgt
     7381 aatcgggaaa tctgtaaaag aagcagagca aatattcaat aaaaacaacc tgaaattggg
     7441 taaaatttct agaagttata gtgataaata tcctgaaaat gaaattatta agacaactcc
     7501 taatacaggt gaacgtgttg aacgtggtga cagtgttgat gttgttatat caaaaggccc
     7561 tgaaaaggtt aaaatgccaa atgtcattgg tttacctaag gaggaagcct tgcagaaatt
     7621 aaaatcgtta ggtcttaaag atgttacgat tgaaaaagta tataataatc aagcgccaaa
     7681 aggatacatt gcaaatcaaa gtgtaaccgc aaatactgaa atcgctattc atgattctaa
     7741 tattaaacta tatgaatctt taggcattaa gcaagtttat gtagaagact ttgagcataa
     7801 atcctttagc aaagctaaaa aagccttaga agaaaaaggg tttaaagttg aaagtaagga
     7861 agagtatagt gacgatattg atgagggtga tgtgatttct caatctccta aaggaaaatc
     7921 agtagatgag gggtcaacga tttcatttgt tgtttctaaa ggtaaaaaaa gtgactcatc
     7981 agatgtcaaa acgacaactg aatcggtaga tgtaccatac actggtaaaa atgataagtc
     8041 acaaaaagtt aaagtttata ttaaagataa agataatgac ggttcaactg aaaaaggtag
     8101 tttcgacatt actagtgatc aacgtataga cattccttta agaattgaaa aaggaaaaac
     8161 agcaagttat attgttaaag ttgacggtaa aactgtagct gaaaaagaag tcagctatga
     8221 tgatgtataa atataattga agtaaatgta ccgaggtttc tatttggaag tctcggtatt
     8281 tttatgttga gattgcggta gttttaaaat gcttcatgtc atcatataca ggtgagcatt
     8341 gtctatcaat tctgtatcac tctcacaata tttatatcat ttgaaatgat catttatata
     8401 ttaattttcc agtctttgat ataataaatg cagtaaaatt aatcgagagg tgccattttg
     8461 aagacaggtc gaatagtgaa atcaattagt ggggtatatc aagtagacgt taatggcgaa
     8521 cgtttcaata caaaaccacg aggattattt agaaagaaaa aattttcacc ggtagttggt
     8581 gatatagtgg aatttgaagt acaaaacatt aacgaaggct atattcatca agtgtttgag
     8641 cggaaaaatg agttgaaaag accacctgta agtaatatag atacactagt aattgtaatg
     8701 agtgctgtcg agccaaattt ttcaacgcaa ttattagatc gatttttagt tattgcacat
     8761 tcgtatcagt taaatgcgag agttttggtg actaaaaaag ataaaacacc aattgaaaag
     8821 cagttcgaaa ttaatgagtt gttgaaaata tatgaaaata ttggctatga gactgaattt
     8881 attggaaatg atgatgatcg aaaaaaaatt gtagaagctt ggccagctgg acttatagta
     8941 cttagtggtc aatcaggtgt cggtaagtcc actttcttaa atcattatcg tccagaactt
     9001 aatcttgaga caaatgatat atcaaaatca ttaaatcgag gaaagcatac tacaagacat
     9061 gtcgaactat tcgaacgtca aaacggttat attgcagaca cacctggatt cagtgcttta
     9121 gattttgatc atatagataa agatgaaata aaagattatt ttcttgaatt aaatcgatat
     9181 ggtgaaacat gtaagtttag gaattgtaat catatcaaag aacctaattg taatgttaag
     9241 catcaattag agatagggaa tattgcgcaa tttagatacg accattattt acaactattt
     9301 aatgaaattt caaatagaaa ggttagatat taaatgacaa aactatatcc atcattatta
     9361 tctgttgatt ttttggattt acaacatgaa ttaaaacgac ttgaagaagc aggtgtcgac
     9421 ggagttcatt ttgatgttat ggatggtcaa tttgtgccta atatatctat tggtttacca
     9481 atattagatg cagtaagaaa aggcacaaca ttacctatag acgtacattt gatgattgaa
     9541 aatccagaaa agtatattgc atcatttgca gaacatggtg ccgatatgat ttcaattcat
     9601 gtcgaatcaa cgcctcatat tcatcgtgct attcaaatga ttaaacattt agataaaaaa
     9661 gctggtgtag taattaatcc tggtacacca atatcacaaa ttgaacctat tttagacatt
     9721 gttgattatg tactagtgat gacagttaac ccagggtttg gtggtcaatc atttattgat
     9781 caatgcgtag aaaaaatagc gggtcttaat gctattaaaa tggaacgtca attaaacttt
     9841 gatattgaag ttgatggagg cgtaaatacc gatacagcga aagtttgtgt tgaaaatggt
     9901 gctacaatgc tagtaacagg ttcatttttc tttaaacaag aggattataa aaaagtcaca
     9961 caagcattga aaggttgagt gtatatgcat ataaatttat tatgttctga tcgacacttg
    10021 ccgcaagata tttgggccaa aagtaatgaa ggtaaatggg gcggcgttga tagaggtgct
    10081 ttgattttat tgaagcatca aattatccct tttttctcag tgggagactt tgattcagtc
    10141 agtaaagaag aacgccaact tctaacagaa cagttacaaa tcaaaccagt tcaagctgaa
    10201 aaagctgata cggatttagc tttagcggtt gataaagctg ttgcacttgg atttgatagt
    10261 attacaattt atggtgcaac aggcggacga ttagatcact tttttggggc aattcagtta
    10321 ttattgaaaa aagcatatta taaacatgat gttcatatag aagttatcga tcaacaaaat
    10381 aaaattgaat tattgcctaa aggtcaacat acagttgaaa aagataagag ttatccgtac
    10441 atttcattta taccgatgac tgatgatgta gaactttctc tagcaggttt taaatataat
    10501 ttagctagac aaatgcttaa tataggttct actttaacta tttcaaatga aattgagtct
    10561 ttgcaagcga aagtaactgt acatgatggg ttgattttgc aaattagaag tacagattta
    10621 aattaagtat attcaaatt
//
LOCUS       DAMZKC010000092        10558 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.093, whole genome shotgun sequence.
ACCESSION   DAMZKC010000092 DAMZKC010000000
VERSION     DAMZKC010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10558
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.093"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            23..934
                     /locus_tag="POB55_001718"
     CDS             23..934
                     /locus_tag="POB55_001718"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HDI6500680.1"
                     /translation="MSKEHTLSQNISRVNFKELQQIIKMGLVQGNLIPAFAGAWLAVV
                     MTNHSFLSSIPQILLMLLGSTLIMGGACALNNYYDQDIDRIMPSKQNRPTVNNRITDQ
                     NLLLLSFGMMLVGEICLFLLNIPSGVLGLMGIVGYVSYYSIWSKRHTTWNTVIGSFPG
                     AVPPLIGWVAIEGQISLTAIALFLVVFCWQPIHFYALAIKRKDEYALANIPMLPSVKG
                     FKRTRVSMFIWLIILLPVPLLLINLGVVFVVLATLLNLGWIALGLTTFKKNSDQTKWA
                     TQMFIYSLNYLVIFFVLAVIVSLLTLI"
     gene            959..1420
                     /locus_tag="POB55_001719"
     CDS             959..1420
                     /locus_tag="POB55_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HDI6500681.1"
                     /translation="MGVPILPTISTTCIVISAILIAIGWRLIWKREINKHKNVMLAAA
                     VFALTFFLIYASRTIFIGNTAFGGPASIKKYYTIFLFFHINLATIGGILGLVQIITAF
                     KDKYNVHRKFGPFASVIWFCTAITGVAVYLLLYVLYPGGETTSLIKATFGH"
     gene            1747..2784
                     /locus_tag="POB55_001720"
     CDS             1747..2784
                     /locus_tag="POB55_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HDI6500682.1"
                     /translation="MKKLIIRVVGVLFLVGFLIYLFYSPRLKFDVLENPNKGNKVNRS
                     EQVNKSNNHAENPKPKEGVGTWVGKDIKVLTSKFGQADRVYPFRDGYKNYVFKDKNSY
                     YIVSTKREEIVSVYATGEKVNVSPLKIGQHSAEIFNHTSINPEPSFKVDGKKYEFELS
                     DEDLKTQTLIKYGDIYAQVYSDQQSKKVLSVRFLTKEMLADIEPYQLNSNSTSEEHNK
                     RPVEQNPNQLISLYEVTNEMRKLKGLKPLKINSDLAHIASNNLYEATSNGSDSVEFTE
                     DALRGQLDKNHVTYKTTAQNVGYAFNDVPTLIHSWMNSDIHRSRLLNSKYDEMGGDVM
                     RDYYSLIFLEK"
     gene            2800..3234
                     /locus_tag="POB55_001721"
     CDS             2800..3234
                     /locus_tag="POB55_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDI6500683.1"
                     /translation="MINEASLAILDDIDELADMIVASDIYASFEQAKQALENNDEAHL
                     LYQSFLKSKEKYDEVMRFGKYHPDYKKVMLETRQRKRAYEMLDVVMHYKAREMALQHL
                     IDEVVTKIAYAVSEHVKIETGNPFFQTSHSGCATGGSCNCSL"
     gene            complement(3297..4223)
                     /locus_tag="POB55_001722"
     CDS             complement(3297..4223)
                     /locus_tag="POB55_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDI6500684.1"
                     /translation="MKKVNRNILNAVLVGAGFVSSLLMINKNKVSTKKQTIPAFFKGN
                     APYIFAHRGGMALRPEQTQLAFDYAKQLGVDGFETDVRLTKDQQLIVFHDATVDRTTN
                     GSGKVSAHTLAELKKLDAAYHFKDINGLTPYRGHAHTAILTFDELLKQYPDMYINVDL
                     KDAPESYEGSIAPQIMFDTIAENQAFDRVLVTSFYKEQIVRFNKIAQGSVAIGASQQE
                     VTEAFLKYHLLGGRYYQPLAQTFQMPTHFKGIDLTSSRFIKWLNDMNIIPGYYGVNSI
                     DLMNDLYQKGAHTIVTDRPDLAQQFKQTIPNK"
     gene            4462..4716
                     /locus_tag="POB55_001723"
     CDS             4462..4716
                     /locus_tag="POB55_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HDI6500685.1"
                     /translation="MNLIPRTSIVVYLKHMKHERQIRKYGHIVHSNRDRKFVIMYVNE
                     QDVDQIVHKLMQLKYVRHIDGSPYKYLKKTYEKEKHEIYN"
     gene            complement(4719..5108)
                     /locus_tag="POB55_001724"
     CDS             complement(4719..5108)
                     /locus_tag="POB55_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500686.1"
                     /translation="MKQTFITLGEGLTDLFEFMTMIEYNHQRIDKIIYFHSPQAENKK
                     SSVAIIMNPTTGNHFQAFYIMINAIKYPYPDSNKKFQMINDCAEKFDIPILGIDVQPP
                     QAFHDLSLYYNYLISVLRLQKWIPELQ"
     gene            5178..5720
                     /gene="rsmD"
                     /locus_tag="POB55_001725"
     CDS             5178..5720
                     /gene="rsmD"
                     /locus_tag="POB55_001725"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002470236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HDI6500687.1"
                     /translation="MRVIAGKHKSKALESMEGRNTRPTMDKVKEGIFNSLYDVSGIGL
                     DLFAGSGALGIEALSRGMDKVIFVDQNFKAVKVIKSNLANLDLEAQSEVYKNNADRAL
                     KALSKRDIQFDVIFLDPPYNKGLIDKALKLISEFNLLKENGIIVCEFSNHEEIDYQPF
                     NMIKRYHYGLTDTLLLEKGE"
     gene            5722..6204
                     /gene="coaD"
                     /locus_tag="POB55_001726"
     CDS             5722..6204
                     /gene="coaD"
                     /locus_tag="POB55_001726"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HDI6500688.1"
                     /translation="MEHTIAVIPGSFDPITYGHLDIIERSTDRFDEIHVCVLKNSKKE
                     GTFSLEERMDLIEQSVKHLPNVKVHQFSGLLVDYCEQVGAKTIIRGLRAVSDFEYELR
                     LTSMNKKLNNEIETLYMMSSTNYSFISSSIVKEVAAYRADISEFVPPYVEKALKKKFK
                     "
     gene            complement(6266..7405)
                     /locus_tag="POB55_001727"
     CDS             complement(6266..7405)
                     /locus_tag="POB55_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HDI6500689.1"
                     /translation="MKSVGLITEYNPFHNGHQYHINQSKKLTNADVTIAIMSGNFVMR
                     GEPAIYNKFTRAKMALSTADLVIELPATASLSSGDHFAELAVKVADYMSVDTIAFGSE
                     NNDIKTLKQLAHSINEIEQSESFSQKVKEGKSYPRIISELLEHHEALASPNNILGISY
                     LKAIAKNAKNINAISIKRENAQHHDSLIQHHQFASGTSIRTSIISQDDHWHHVVPKDI
                     QHLYVTPHITLNQIFPYLKYQIIAMTTDSLKNIYTVTEGFENRLKSNIYEATDFHHFV
                     KLLKTKRYTYTHIQRLLMNVLLNIKPTDVTSNIHAVKVLAMNDRGRQYLKHLKTAFPE
                     RQYITNINKSNAHYFTNEIKATHIYNAISGQQQTDFNTPVIQQYR"
     gene            7532..8089
                     /locus_tag="POB55_001728"
     CDS             7532..8089
                     /locus_tag="POB55_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HDI6500690.1"
                     /translation="MKWSITQLRKYQGKPFEFDQTVSFDNLKESLDLIDLSPITIQGQ
                     LTIKSTEVVADIHITGTYTMPCARTLVPVKVPLDVTTTEVFDLEGYNQYNDDQDDVDE
                     HYHIIKDGMVNLQDIVEDIVIIEKPMRAYSEQSDQMLTVGNGWEVIDEDQLDELAKQQ
                     EQDDSESRQVDPRLQKLQQLYDKEQ"
     gene            8169..8342
                     /gene="rpmF"
                     /locus_tag="POB55_001729"
     CDS             8169..8342
                     /gene="rpmF"
                     /locus_tag="POB55_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019469283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDI6500691.1"
                     /translation="MAVPKRRTSKTRKNKRRTHFKISVPGMTECPNCGEYKLSHRVCK
                     NCGSYNGEEVAAK"
     gene            complement(8496..10433)
                     /gene="isdB"
                     /locus_tag="POB55_001730"
     CDS             complement(8496..10433)
                     /gene="isdB"
                     /locus_tag="POB55_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme uptake protein IsdB"
                     /protein_id="HDI6500692.1"
                     /translation="MNKQQKEFKSFYSIRKSSLGVASVAISTLLLLMSNGEAQAAAEE
                     TGGTNTEAQPKTEAVASPTTTSEKAPETKPVANAVSVSNKEVEAPTSETKEAKEVKEV
                     KAPKETKEVKPAAKATNNTYPILNQELREAIKNPAIKDKDHSAPNSRPIDFEMKKKDG
                     TQQFYHYASSVKPARVIFTDSKPEIELGLQSGQFWRKFEVYEGDKKLPIKLVSYDTVK
                     DYAYIRFSVSNGTKAVKIVSSTHFNNKEEKYDYTLMEFAQPIYNSADKFKTEEDYKAE
                     KLLAPYKKAKTLERQVYELNKIQDKLPEKLKAEYKKKLEDTKKALDEQVKSAITEFQK
                     VQPTNEKMTDLQDTKYVVYESVENNESMMDTFVKHPIKTGMLNGKKYMVMETTNDDYW
                     KDFMVEGQRVRTISKDAKNNTRTIIFPYVEGKTLYDAIVKVHVKTIDYDGQYHVRIVD
                     KEAFTKANTDKSNKKEQQDNSAKKEATPATPSKPTPSPVEKESQKQDSQKDDNKQLPS
                     VEKENDASSESGKDKTPATKPTKGEVESSSTTPTKVVSTTQNVAKPTTASSKTTKDVV
                     QTSAGSSEAKDSAPLQKANIKNTNDGHTQSQNNKNTQENKAKSLPQTGEESNKDMTLP
                     LMALLALSSIVAFVLPRKRKN"
BASE COUNT         3400 a         1487 c         1862 g         3809 t
ORIGIN      
        1 aacaaattta aggaggggga ttatgagcaa agagcatact ttgtcacaaa atattagcag
       61 agttaacttc aaagaattgc aacagataat taaaatggga cttgttcaag gtaacttaat
      121 tccggctttt gcgggcgcat ggctagcagt tgtaatgaca aatcattcct tcttatcatc
      181 aatacctcaa attttattaa tgctattagg atctacttta attatgggtg gcgcatgtgc
      241 gttaaataat tattacgacc aagatattga tcgtattatg cctagtaaac aaaatagacc
      301 aactgtaaat aatagaatta cagatcaaaa tttattatta ttaagttttg gtatgatgtt
      361 agttggagaa atttgtttgt ttttattgaa tataccatca ggcgtacttg gtcttatggg
      421 gattgtaggt tatgtgtctt attactcaat atggtctaaa agacatacaa catggaacac
      481 agtgattggg agttttcctg gagcagtacc accactaatt ggatgggttg caattgaagg
      541 acaaattagt ttaacagcga ttgcgctgtt tttagttgta ttttgttggc aaccaattca
      601 tttttatgcc ttagctatta aacgtaaaga tgaatatgca cttgcaaata ttccaatgtt
      661 accatcagtt aaaggcttta aacgtacacg tgtcagtatg tttatctggt tgattatttt
      721 attgccagta cctttattac taataaattt aggtgtcgta ttcgtagtgt tagctacatt
      781 attaaattta ggatggattg cattaggttt aacaacattt aagaaaaatt cagatcaaac
      841 aaaatgggca acacaaatgt ttatatattc actaaattat ttagtgatct ttttcgtgtt
      901 agctgtgatt gtttcattac ttactttgat ctagattaat taagttagga tgaaaaatat
      961 gggcgttcca attttaccaa cgattagtac gacatgtatt gtcattagtg caattttaat
     1021 tgccattggt tggagactta tttggaaaag ggaaataaat aaacacaaaa atgttatgtt
     1081 agctgcagct gtttttgctt taactttttt cttaatctat gcaagtagaa cgattttcat
     1141 cggtaataca gcatttggcg gaccagcatc aattaagaaa tattatacga ttttcttatt
     1201 tttccacatt aatttagcaa caattggtgg tattctaggc ctagttcaaa ttattactgc
     1261 atttaaagat aaatataatg tacaccgcaa attcgggcca ttcgcttcag ttatatggtt
     1321 ctgtactgca attacaggtg tagcagttta cttattatta tatgtattat atccaggtgg
     1381 agaaacgaca tcactgatta aagcaacatt tggtcattaa tatgcttata caaatgatac
     1441 taatctgata aggcaatttg ttttatcagg ttttttgatg aagaatagtc ggacagttga
     1501 tggtattcat ttgatacctt aactgtccat ttttttatac atattttaat cctaataatg
     1561 aaattcaacc tgaaaacata aagataggtt atgatataga gtgtagggaa aatctttatg
     1621 acgttaaaat tatgcctgaa gatgattttg tttcatcgaa acgacacagt tatgtcagtg
     1681 ttgaaaattt gctaattaat gtgttgtttt ttacaatagt attaatactt tttaggtggt
     1741 tgattgatga aaaagttaat tataagagtc gtcggtgtat tatttttagt tggattttta
     1801 atatatcttt tttattcacc gagattaaaa tttgatgtat tagagaaccc gaataaaggt
     1861 aataaagtaa atagatctga acaggtgaat aaatcaaata accatgctga aaatccaaag
     1921 cctaaagaag gtgttggtac atgggtaggt aaagatatta aagtgcttac ttctaaattt
     1981 ggacaagcag atcgtgtcta cccttttaga gatggttaca aaaattatgt gtttaaagac
     2041 aaaaacagtt attacattgt ttcaactaaa cgtgaagaaa tcgtttcagt gtatgctaca
     2101 ggtgagaaag tcaatgttag tccgttaaaa ataggacaac attctgcaga aatttttaat
     2161 catacaagta ttaatccaga accgtccttt aaagttgatg gtaaaaaata tgaatttgaa
     2221 ctttcagatg aagatttaaa aacacaaaca ctgattaaat atggcgacat atatgcacaa
     2281 gtgtattctg atcaacaatc gaaaaaggtg ctaagtgtac gatttttaac aaaagaaatg
     2341 ttagcagata ttgaacctta tcaattaaat tctaattcta cgtcagaaga gcataataag
     2401 cgtccagttg agcaaaatcc aaatcaatta atttctcttt atgaagtaac gaatgaaatg
     2461 agaaaattaa aaggattaaa accattgaaa atcaatagcg atttagcaca tattgcatct
     2521 aataacttat atgaagcgac ctctaatggt tctgatagtg ttgaatttac agaggacgca
     2581 ttaagagggc aattagataa aaatcacgtt acttataaaa caactgctca aaatgttggt
     2641 tatgcgttta atgatgtacc aacattaatc catagttgga tgaattcaga tatacatcga
     2701 tctcgtctat taaattcaaa atacgatgag atgggtggag atgtaatgag agattattat
     2761 tcactaattt tcttagaaaa ataacaatga ggggtattta tgattaatga agcttcacta
     2821 gcgatattag atgatattga tgaactagct gatatgatag ttgcatcaga tatttatgca
     2881 tcatttgagc aagctaaaca ggcgctcgaa aacaatgacg aagcacattt actttatcaa
     2941 tcatttttaa aatcaaaaga gaaatatgat gaagtaatgc gtttcgggaa atatcatcct
     3001 gattataaga aagtcatgtt agagacaaga caacgcaaaa gagcatatga gatgcttgac
     3061 gttgtgatgc attacaaagc tagggaaatg gcacttcaac atttaattga tgaagttgtt
     3121 acgaaaattg cgtacgctgt ttcagagcat gtcaaaatag aaacaggtaa tccattcttt
     3181 caaacatcac atagtggttg tgcgacgggc ggatcctgta attgttcatt ataaaaaaca
     3241 tcgagtcaga aaaaggtggt tattgaaacc actaactagc atctgactcg atgtttttat
     3301 ttattcggga ttgtttgttt gaattgttgt gctaaatctg gtcgatctgt cacaatcgtg
     3361 tgtgcacctt tttggtataa atcattcatc agatctatac tatttacgcc ataatagcct
     3421 ggaatgatat tcatatcatt taaccatttg ataaaacgag atgaagtcaa atcaatgcct
     3481 ttaaaatgag taggcatttg gaacgtttgt gctaatggtt ggtagtatct accacctaat
     3541 aaatgatatt ttaaaaatgc ttctgtaact tcctgttggc tagcaccaat tgcgacggat
     3601 ccttgtgcaa ttttattaaa acgaacgatt tgttctttat aaaaacttgt cacaagaacg
     3661 cggtcaaatg cttgattttc tgcaattgta tcaaacataa tttgtggtgc gattgagcct
     3721 tcataggatt caggagcatc ttttaagtct acgtttatat acatatcagg atattgcttc
     3781 agcaactcat cgaaggttag tatagctgtg tgtgcatgac cacgatatgg tgttagtcca
     3841 ttgatatctt tgaagtgata agctgcgtct aatttcttta attctgctaa tgtatgggca
     3901 ctaacttttc cagagccgtt cgtcgttcta tcaacagttg cgtcatgaaa aacgataagc
     3961 tgttgatctt ttgtgagtct cacatccgtt tcaaagccat caacgcctaa ttgtttagca
     4021 tagtcaaatg caagttgcgt ttgctctggt cttaaagcca taccaccgcg atgcgcaaat
     4081 atatatggtg cattgccttt gaaaaaagca gggatggttt gctttttagt actcacttta
     4141 tttttattga tcattaatag actacttaca aatccagcac cgactagtac cgcatttaaa
     4201 atgtttctgt ttactttttt catagaaaat tcctcctcgt ttacttttat agcttaacaa
     4261 aatcatagtt ctgactacag tgaaatgtgt ggttaatcaa atagtgatgc tcttgagaat
     4321 tgaattaata taatgatgtt taatataatc taaatacatt tatcgagcaa ttacacaaga
     4381 catatttaag gtagcttaat attttaaaat taataaagac atgatatgat taatgctgaa
     4441 aataaatgga gtgaggttta aatgaattta atcccaagaa ctagtattgt agtttattta
     4501 aaacatatga aacatgaacg acaaatccga aaatatggac atatcgttca ttcaaataga
     4561 gatcgtaaat ttgtaattat gtatgtgaat gagcaagatg ttgatcaaat tgtacataaa
     4621 ctaatgcaac ttaaatacgt tagacacatt gatggctcac catataaata cttaaagaaa
     4681 acttacgaaa aagagaaaca cgaaatatat aattaatatt attgaagttc tggtatccat
     4741 ttttggagcc ttaacacact aattaaataa ttataatata acgataaatc atgaaatgct
     4801 tgagggggct gtacatcgat acctaaaatt ggtatgtcga atttttcagc acaatcatta
     4861 atcatttgaa actttttatt tgaatctgga tatggatatt taatagcgtt aatcatgata
     4921 taaaatgctt ggaaatgatt gccagttgta gggttcataa tgattgctac agatgacttt
     4981 ttattttcag cttgtggtga atgaaaatag ataattttat caatacgttg atggttatat
     5041 tcaatcatcg tcatgaattc gaacaaatct gttagacctt caccaagtgt aataaatgtt
     5101 tgtttcattg gtttatcatt ctcctttttc tcaataaatt atattaaata actaaatgaa
     5161 ttggaagtgt gaacaacatg cgcgtcattg caggtaaaca taaaagtaaa gctttagaaa
     5221 gtatggaagg ccgtaatacg agaccaacta tggataaagt taaagaaggt atctttaata
     5281 gtttatatga tgtgtcaggt ataggtttag atttatttgc aggaagtgga gcgcttggaa
     5341 tagaagcact ctctcgaggt atggataagg taatctttgt tgatcaaaat tttaaagctg
     5401 taaaagttat taaatcaaat cttgcgaatt tggatttaga ggcacaatct gaagtttata
     5461 aaaataatgc agatagagct ttaaaagcat tgtcaaaacg ggatattcaa tttgatgtca
     5521 ttttcttaga tccaccttat aataaaggtc tcattgataa agctttaaaa ctaatttcag
     5581 agtttaattt attgaaagaa aatggtatca tcgtttgtga atttagcaat catgaagaaa
     5641 tagactatca accgtttaat atgattaaac gttaccatta tgggttgaca gacacattgt
     5701 tattagaaaa gggagaatag catggaacat acaatagcgg tcattccggg tagttttgac
     5761 cccattactt atggtcattt agacattatt gagagaagta cagatagatt tgatgaaatt
     5821 catgtctgtg ttcttaaaaa tagtaaaaaa gaaggtacgt ttagtttaga agagcgtatg
     5881 gatttaattg aacaatctgt taaacattta cctaatgtca aggttcatca atttagtggt
     5941 ttactagtcg attattgtga acaagtagga gctaaaacaa tcatacgtgg tttaagagca
     6001 gtcagtgatt ttgaatatga attacgctta acttcaatga ataaaaagtt gaacaatgaa
     6061 attgaaacgt tatatatgat gtctagtact aattattcat ttataagttc aagtattgtt
     6121 aaagaagttg cagcttatcg agcagatatt tctgaattcg ttccacctta tgttgaaaag
     6181 gcattgaaga agaaatttaa gtaataaaaa taacagtatt ttaggtttat catggtttac
     6241 aatcctaaaa tactgttttc atttgttaac gatattgctg tatgacaggc gtgttgaaat
     6301 ctgtttgttg ttgcccgctt attgcattgt atatgtgtgt tgctttgatt tcatttgtga
     6361 agtaatgtgc attgcttttg ttaatattgg ttatatattg tctttctggg aacgctgttt
     6421 ttaaatgctt taaatattgt ctgccacggt cgttcatcgc taatacttta actgcgtgaa
     6481 tgttactcgt aacatctgta ggtttaatgt ttaataatac attcattaac agtctttgga
     6541 tatgcgtata tgtataacgc tttgttttta gtaattttac aaaatgatga aaatcagttg
     6601 cttcataaat gttagatttc aaacgatttt caaaaccttc agtaacagta taaatatttt
     6661 ttaatgaatc tgtagtcata gctatgattt gatatttcaa atatggaaat atttgattta
     6721 atgttatatg aggtgttacg tacaagtgtt gaatatcttt aggtaccaca tgatgccaat
     6781 gatcatcttg actaatgatt gatgttctaa tagatgtacc acttgcaaac tgatggtgtt
     6841 gaattaatga atcatgatgt tgagcatttt ctcgtttgat agaaattgca ttgatgtttt
     6901 tagcattttt agcaattgct ttcaggtaac taataccaag tatgttgtta ggacttgcta
     6961 gtgcttcatg atgctctaat aattcgctaa tgatacgagg gtagctttta ccttctttta
     7021 ctttttgtga aaaggattca gattgttcaa tttcattaat gctgtgtgct aattgcttta
     7081 atgttttgat atcattattt tcactaccaa atgcaattgt atcgacactc atataatctg
     7141 cgactttaac tgctagttcg gcaaaatgat cgccagatga taaactggca gttgctggta
     7201 gttcgataac taaatcagct gttgataacg ccatttttgc acgagtaaac ttattataga
     7261 ttgctggttc gccacgcatg acaaagttac cactcattat tgcaatagta acgtcagcat
     7321 ttgtaagttt tttagattga ttaatatgat attgatgccc attatgaaag ggattatatt
     7381 ctgtgattaa gccaacgctt ttcatttgca attcgtcctt tcggtattca ttattaatat
     7441 tgtaacagaa catgatatgt taagaaaaaa tcttgacaac ttgttcttag aaagttaaaa
     7501 taaattttgt gcttgttaga ggtgaagcca tatgaaatgg tcaattacgc aattaaggaa
     7561 atatcaaggt aagccatttg aatttgatca aacggtgagt tttgacaatt taaaagaatc
     7621 attagattta attgatttat ctccaattac aatccaaggt cagttaacca ttaagtcaac
     7681 agaagtcgtt gcggatattc acattactgg aacgtataca atgccttgtg cacgtactct
     7741 tgtaccagta aaagtcccac tagatgtaac tactacagaa gtatttgatt tagaagggta
     7801 caatcagtat aacgatgatc aagatgatgt agatgaacac tatcacatta ttaaagatgg
     7861 tatggttaat cttcaggata ttgtcgagga tatagttatt attgagaaac caatgagagc
     7921 ttattcagag caaagtgacc aaatgttgac agtaggtaat ggttgggaag taatcgatga
     7981 ggatcaatta gatgagcttg ctaaacagca agaacaagat gattcagaat cacgacaagt
     8041 tgatccaagg cttcaaaaat tacaacaatt atatgataaa gagcaataag tagtttaatt
     8101 aatgatataa tgttgttaat taaactaaca tttagtattt ttcgtttata atctaaggag
     8161 gatatatcat ggcagtacca aaaagaagaa cttctaaaac tagaaaaaac aaacgtcgta
     8221 cgcatttcaa aatttcagta ccaggtatga ctgaatgccc aaactgtggc gaatacaaat
     8281 tatcacaccg tgtatgtaaa aactgtggtt cttacaatgg cgaagaagta gcagctaaat
     8341 aattttagtt actcaatata aaaagtcccg cttaaaatga ttgttttaag tgggactttt
     8401 tatattgcga aaaataattg gcgaacgagg taactggata cctcatccgc caattaaaat
     8461 ttgttaattt aataattaaa tataaagacg atttattagt ttttacgttt tctaggtaat
     8521 acgaatgcaa cgatgctact taaagctaat aatgccatta atggtaatgt catatcttta
     8581 tttgattctt caccagtttg tggtaatgat tttgctttat tttcttgtgt atttttattg
     8641 ttttggcttt gagtgtgtcc atcatttgtg tttttaatgt ttgctttttg taatggagca
     8701 ctatcttttg cttcgctaga acctgctgaa gtttgaacaa catcttttgt tgtttttgat
     8761 gaagcagttg ttggttttgc aacattttga gtcgtagata ctaccttagt tggagttgta
     8821 ctacttgatt ctacttcacc tttagttggt tttgtagcag gcgttttgtc tttacctgac
     8881 tcactagatg cgtcattttc tttttcaaca cttggtaatt gtttattgtc atctttttgg
     8941 ctgtcttgtt tttgtgattc tttttcaaca ggtgatggtg ttggtttgct aggcgtagct
     9001 ggagtagctt ccttcttagc tgagttatct tgttgttctt ttttgttaga tttatcggta
     9061 ttggcttttg taaatgcttc tttatcaacg attctgacat ggtattgtcc atcataatca
     9121 atcgttttta cgtgaacttt aacgatagca tcatatagag ttttaccttc aacatatggg
     9181 aaaataattg ttctagtatt atttttagca tctttgctta tagttctaac acgttgacct
     9241 tcaaccatga aatctttcca gtaatcgtca ttagtagttt ccatgaccat atattttttg
     9301 ccgttaagca tacctgtttt aatagggtgt ttaacaaaag tatccatcat agattcgtta
     9361 ttctcaacac tttcataaac aacatatttt gtatcttgta aatcagtcat tttttcattt
     9421 gttggttgta ctttttggaa ttcagtaata gctgatttca cttgctcatc taaagctttc
     9481 tttgtatcct ctaatttctt cttgtactca gcctttaatt tttcaggaag tttatcttga
     9541 attttattta attcataaac ttgtctttct agtgttttcg cttttttata tggcgctaat
     9601 aatttttcag ctttataatc ttcttcagtt ttgaatttat ctgcactgtt ataaattggt
     9661 tgtgcgaatt ccattaatgt gtaatcgtat ttttcttctt tgttattgaa gtgtgttgaa
     9721 ctaacaattt taacagcttt tgttccgttt gatacagaga agcgaatgta agcataatct
     9781 ttaacagtat cgtatgatac taatttaatt ggcaactttt tgtcaccttc ataaacttca
     9841 aattttctcc aaaattgacc tgattgtaat cctaattcaa tttctggttt tgaatcagtg
     9901 aaaataactc tagcaggttt aacagaactt gcataatgat aaaactgttg agttccatct
     9961 ttctttttca tttcaaaatc aattggacga gagtttggtg cgctatgatc tttgtctttt
    10021 attgcagggt ttttaatcgc ttctctaagt tcctgattca aaataggata tgtattgtta
    10081 gtggcttttg ctgctggttt aacttctttt gtttccttag gggctttaac ttctttaact
    10141 tctttagctt cttttgtttc agaagtagga gcctcaactt ctttattaga tactgagaca
    10201 gcattagcta ctggtttagt ttctggagct ttttcagatg ttgttgttgg acttgcaact
    10261 gcttcagttt ttggttgtgc ttctgtattt gtaccacctg tttcttcagc tgctgcttgt
    10321 gcttcgccat ttgacattaa taataaaagt gtactaattg ctacagatgc aacgcctagt
    10381 gatgactttc taattgaata aaatgattta aattcttttt gctgtttgtt catgttgtaa
    10441 aaacaactcc taattgtata ttatcaactg ataatcatta tcaattgatt acttgtattg
    10501 tacattgaaa tggtagttaa ttgcaacgtt attttataaa aaaataacct atgttata
//
LOCUS       DAMZKC010000093        10507 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.094, whole genome shotgun sequence.
ACCESSION   DAMZKC010000093 DAMZKC010000000
VERSION     DAMZKC010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10507
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.094"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(101..727)
                     /locus_tag="POB55_001731"
     CDS             complement(101..727)
                     /locus_tag="POB55_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HDI6500693.1"
                     /translation="MAKVLYITAHPFNELVSNSMAAGKAFIETYQQQHPDDEVKHIDL
                     FETYIPVIDKDVLTGWGKMSNGETLTDDEQMKVSRLSDILEEFLSADKYVFVTPMWNL
                     SFPPVVKAYIDAISIAGKTFKYSAEGPQGLLTDKKVLHIQSRGGYYTEGPAADFEMGD
                     RYLRTIMTFLGVPSYETIIIEGHNAEPHKTEEIKATSINNAEKLATIF"
     gene            936..1514
                     /locus_tag="POB55_001732"
     CDS             936..1514
                     /locus_tag="POB55_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HDI6500694.1"
                     /translation="MTKRPKRILATIIIFLSLLFTIIYIDDIQKWFNQYTDKLTQNHK
                     GQGHSKWEDFFRGSRITETFGKYQHSPFDGKHYGIDFALPKGTPIKAPTNGKVTRIFN
                     NELGGKVLQIAEDNGEYHQWYLHLDKYNVKVGDRVKAGDIIAYSGNTGKQTTGAHLHF
                     QRMKGGVGNAYAEDPKPFIDQLPDGERSLYDL"
     gene            1897..2994
                     /gene="ugpC"
                     /locus_tag="POB55_001733"
     CDS             1897..2994
                     /gene="ugpC"
                     /locus_tag="POB55_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HDI6500695.1"
                     /translation="MAELKLEHIKKTYDNNNTVVKDFNLHITDKEFIVFVGPSGCGKS
                     TTLRMVAGLESITSGDFYIDGERMNDVEPKNRDIAMVFQNYALYPHMTVFENMAFGLK
                     LRKVNKKEIEQKVNEAAEILGLTEYLGRKPKALSGGQRQRVALGRAIVRDAKVFLMDE
                     PLSNLDAKLRVQMRTEILKLHKRLNTTTIYVTHDQTEALTMASRIVVLKDGDIMQVGT
                     PREIYDAPNCIFVAQFIGSPAMNMLNATVEMDGLKVGTHHFKLHNKKFEKLKAAGYLD
                     KEIILGIRAEDIHEEPIFIQTSPETQFESEVVVSELLGSEIMVHSTFQGMELISKLDS
                     RTQVMANDKITLAFDMNKCHFFDEKTGNRIV"
     gene            3007..4278
                     /locus_tag="POB55_001734"
     CDS             3007..4278
                     /locus_tag="POB55_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDI6500696.1"
                     /translation="MSKILKCITLAVVMLLIVTACGPNRSKEDIDKALNKDNSKDKPN
                     QLTMWVDGDKQMAFYKKITDQYTKKTGIKVKLVNIGQNDQLENISLDAPAGKGPDIFF
                     LAHDNTGSAYLQGLAAEIKLSKDELKGFNKQALKAMNYDNKQLALPAIVETTALFYNK
                     KLVKNAPQTLEEVEANAAKLTDSKKKQYGMLFDAKNFYFNYPFLFGNDDYIFKKNGSE
                     YDIHQLGLNSKRVVKNAERLQKWYDKGYLPKAATHDVMIGLFKEGKVGQFVTGPWNIN
                     EYQETFGKDLGVTTLPTDGGKPMKPFLGVRGWYLSEYSKHKYWAKDLMLYITSKDTLQ
                     KYTDEMSEITGRVDVKSSNPNLKVFEKQARHAEPMPNIPEMRQVWEPMGNASIFISNG
                     KNPKQALDEATNDITQNIKILHPSQNDKKGD"
     gene            4281..5549
                     /locus_tag="POB55_001735"
     CDS             4281..5549
                     /locus_tag="POB55_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDI6500697.1"
                     /translation="MTKRNPKLAALLSVIPGLGQFYNKRPIKGTIFFIFFISFISVFY
                     SFLNIGFWGLFTLGTVPKLDDSRVLLAQGIISILLVAFAIMLYIINILDAYRNAERFN
                     RNEEIKDPKARMVATWDKTFPYLLISPGTFLLIFVVVFPLIFMFGVAFTNYNLYNAPP
                     RHTLEWVGLDNFKTLFTIGVWRKTFFSVITWTLVWTLVATTLQIALGLFLAIIVNHPV
                     VKGKKFIRTVLILPWAVPSFVTILIFVALFNDEFGAINNDILQPLLGVAPAWLSDPFW
                     AKVALIGIQVWLGFPFVFALFTGVLQSISSDWYEAADMDGASSWQKFRNITFPHVIYA
                     TAPLLIMQYAGNFNNFNLIYLFNKGGPPVSGQNAGSTDILISWVYNLTFEFNNFNMGA
                     VVSLIIGFIVAIVAFIQFRRTSTFKDEGGL"
     gene            5551..6390
                     /locus_tag="POB55_001736"
     CDS             5551..6390
                     /locus_tag="POB55_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDI6500698.1"
                     /translation="MTKKKNILKAIGIYSFIAMMFVIILYPLLWTFGISLNPGTNLYG
                     AKMIPDNATFKNYAFLLFDDSSQYLTWYKNTLIVASANALFSVIFVTLTAYAFSRYRF
                     VGRKYGLITFLILQMFPVLMAMVAIYILLNTIGLLDSLFGLTLVYIGGSIPMNAFLVK
                     GYFDTIPKELDESAKIDGAGHMRIFLQIMLPLAKPILAVVALFNFMGPFMDFILPKIL
                     LRSPEKFTLAVGLFNFINDKYANNFTVFAAGAIMIAVPIAIVFLFLQRYLVSGLTTGA
                     TKG"
     gene            6464..7540
                     /locus_tag="POB55_001737"
     CDS             6464..7540
                     /locus_tag="POB55_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDI6500699.1"
                     /translation="MTIKVGIIGCGGIANGKHMPSLQKVENVEMIAFCDVDISKAASA
                     AEAYGTDNAKVYDDYKALLKDDTIDVIHVCTPNDSHCEITVAGLHAGKHVMCEKPMAK
                     TTAEAQKMIDTAKSTGKKLTIGYQNRFRADSQFLHQAAQRGDLGDIYFGKAHAIRRRA
                     VPTWGVFLDEEAQGGGPLIDIGTHALDLTLWMMDNYEPESVMGSTFHKLNKQHHAANA
                     WGSWNPDEFTVEDSAFGFIKMKNGATIMLESAWAINSLEVDEAKCSLSGTKAGADMKD
                     GLRIHGEDMGTLYTKHVELENKGVDFYEGNEVDEAEEEAKAWIDAVVNDTEPVVKPEQ
                     AMVVTKILEAIYQSAKSGKAIYFE"
     gene            7565..8605
                     /locus_tag="POB55_001738"
     CDS             7565..8605
                     /locus_tag="POB55_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDI6500700.1"
                     /translation="MTKLKVGVIGVGGIAQDRHIPALLKLKDTVSLVAVQDINTVQMI
                     DVAKRFNIPHAVETPSELFKLVDAVVICTPNKFHADLSIEALNHGVHVLCEKPMAMTT
                     EECDRMIEAANKNHKLLTVAYHYRHTDVAITAKKAIESGVVGKPLVARVQAMRRRKVP
                     GWGVFTNKALQGGGSLIDYGCHLLDLSLWLLGKDMVPHEVLGKTYNQLSKQPNQINDW
                     GTFDHTKFDVDDHVTSYMTFANRASMQFECSWSANIKEDKVHVSLSGEDGGINLFPFE
                     IYEPRFGTIFESKANVEHNEDIAGERQARNFVNACLGIEEIVVKPEEARNVNALIEAI
                     YRSDLDNKSIQL"
     gene            8660..9628
                     /locus_tag="POB55_001739"
     CDS             8660..9628
                     /locus_tag="POB55_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDI6500701.1"
                     /translation="MKIGVFSVLFYDKNFEDMLDYVSESGLDMIEVGTGGNPGDKFCK
                     LDELLENEDKRQAFMKSITDRGLQISGFSCHNNPISPDPIEAKEADETLRKTIRLANL
                     LDVPVVNTFSGIAGSDDTAKKPNWPVTPWPTAYSEIYDYQWNEKLIPYWQDLAEFAKE
                     QDVKIAIELHAGFLVHTPYTMLKLREATNEYIGANLDPSHLWWQGIDPIAAIRILGQA
                     NAIHHFHAKDTYINQENVNMYGLTDMQPYGNVATRAWTFRTVGYGHSPYVWADIISQL
                     IINGYDYVLSIEHEDPIMSVEEGFQKACQTLKSVNIYDKPADMWWA"
     gene            complement(9989..10483)
                     /locus_tag="POB55_001740"
     CDS             complement(9989..10483)
                     /locus_tag="POB55_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002454321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HDI6500702.1"
                     /translation="MILSILLIFFCIRLVSLKISINHSKQLKADGAVEYGVKNSKFLA
                     ITHVLIYVLAGVEAFINKDTFSFANGIGLVILIFAYIMLFMVIKTLGGIWTLKLFILP
                     NHPIIKSGLYKITKHPNYFLNIIPELIGVLLLTHATYTTILLVPYAYFLYVRIKQEEK
                     LMNI"
BASE COUNT         3578 a         1597 c         2065 g         3267 t
ORIGIN      
        1 tttaaatgtg tggaaataat aggtatttat aaaaaagtag gaaatgcata caattaaaaa
       61 ctgtgtgacg tttgagacgt cacacagtgt aactaaaaat ttaaaaaatt gttgctaatt
      121 tttcagcatt attaatacta gttgctttaa tttcttcagt cttatgaggt tcagcattgt
      181 gtccttcaat aatgattgtt tcatatgatg gcacacctaa gaatgtcata attgttctta
      241 aataacggtc acccatttca aaatcagcag caggtccttc agtataatat ccaccacgtg
      301 attgaatgtg taatactttt ttgtcagtta gtaaaccttg tggtccttca gcagaatatt
      361 taaaagtttt acctgcaatt gaaatagcat caatatatgc tttaactaca ggtgggaaag
      421 aaaggttcca cataggcgtt acaaatacat atttatctgc acttaaaaat tcttctaaaa
      481 tgtcactcaa tcttgaaact ttcatttgtt catcatcagt taacgtttcg ccattactca
      541 tttttcccca accagttaat acatctttgt caataactgg aatataagtt tcaaataaat
      601 caatatgttt cacttcatca tcaggatgtt gttgttgata tgtttcgata aatgctttac
      661 cagccgccat agaatttgat accagttcat taaaagggtg tgctgtaata tataatactt
      721 ttgccatttg aaaattctcc tctgtttctg ttattttctt gagtataatt attatactag
      781 atataaaatt taatatcaat aaaaatattc aaattaccat cattttcttc atctatattt
      841 ggcagtacta ctaaagtatg agtgcattta attatgaaat agttgattta gaatatatac
      901 ttaataccca aaatatatga aggatggatg ccactatgac aaagcgacca aaacgtattt
      961 tggcaacaat tatcattttt ctttcactat tatttacgat tatttatata gatgacattc
     1021 aaaaatggtt taaccaatat accgataaat tgacacaaaa tcataaagga caaggacact
     1081 caaaatggga agactttttt agagggagtc ggattactga gacttttggt aaatatcaac
     1141 attcaccatt tgatggtaag cattatggca ttgattttgc attgccaaaa ggtacaccaa
     1201 ttaaagcgcc gacgaatggt aaagtaacac gtatctttaa taatgaattg ggcggcaagg
     1261 tattacagat tgccgaagac aatggagaat atcaccagtg gtatctacac ttagacaaat
     1321 ataatgtcaa agtaggtgat cgagtcaaag caggtgatat tattgcatat tcaggcaata
     1381 caggtaaaca aacgacaggc gcacatttac attttcaaag aatgaagggt ggcgtaggta
     1441 atgcatatgc agaagatcca aaaccgttta tcgatcagtt acctgatggg gaacgtagcc
     1501 tatatgattt gtagttatag aagggtgccc gcagtctaaa aaattaagca atcattgtgt
     1561 gagtatgata cttacataat ggttgctttt ttcaatgaaa atcgtaatgc taagtcatac
     1621 ttgtttgatt tagatattac ttaaaatgta agacaaggtt gttagcattg gcagtgaaat
     1681 atcgcacata aaaaacatta ttgtcacact agaaaatagt tgtgcactat atcaattttc
     1741 tgtataaaag tttaattctg acagtaatgt aaacgtttac aatttatgat tgacattaat
     1801 aatgactgaa tatatgattt atgtaagtat ttgtgcaaac gttttcacaa agtgtattgc
     1861 acaatcaaac tgtaaacaaa gtatgggagg cataacatgg cagaactaaa gttagagcat
     1921 attaaaaaga cgtatgataa caacaatact gtagtgaaag attttaatct acatattact
     1981 gacaaagaat tcattgtatt tgttggacca tcgggatgtg gtaaatcaac aacattacga
     2041 atggttgctg gactagagtc tatcacatct ggagattttt atattgatgg ggaacgcatg
     2101 aacgatgttg aaccaaagaa tagagatatt gcgatggtat ttcaaaacta tgcattatat
     2161 ccacatatga ctgtttttga aaatatggca tttgggctaa agctacgtaa agtaaataaa
     2221 aaagagattg aacaaaaagt taatgaagca gctgaaatat taggattaac tgagtatctt
     2281 ggtcgtaaac caaaagcgtt atctggcgga cagcgtcaac gtgttgcttt gggcagagct
     2341 attgttaggg atgcgaaagt ctttttaatg gatgaaccat tatcgaatct tgatgcgaag
     2401 cttcgagtac aaatgcgcac agaaatattg aaattacata agcgacttaa tactacgaca
     2461 atttatgtta cacatgatca aactgaagca ttgacgatgg ctagtcgaat tgttgttttg
     2521 aaagatggcg acattatgca agtcggcaca cctagagaaa tatatgatgc ccctaattgc
     2581 atatttgtgg cgcaatttat cggctcacca gcaatgaata tgttgaatgc tacagttgaa
     2641 atggacggat tgaaggtagg aacacaccat tttaaattac ataataaaaa atttgaaaag
     2701 ttaaaagctg ctggctactt agacaaggaa attattttag gtattcgagc tgaagacatt
     2761 catgaagaac caatatttat tcaaacttct ccagagacac aatttgaatc tgaagtagtt
     2821 gtatccgaac tgttaggttc agaaattatg gtacatagca cattccaagg aatggaattg
     2881 atttctaaat tagattcaag aactcaagtg atggcgaacg acaagattac actagcattt
     2941 gatatgaata agtgtcactt ttttgatgaa aaaacaggaa atcgtatcgt ctaaggggga
     3001 gtattcatgt ctaaaatttt aaaatgtatc acgttagccg tggtaatgtt attaatcgta
     3061 actgcatgtg gccctaatcg ttcgaaagaa gatattgata aagcattgaa taaagataat
     3121 tctaaagaca agcctaacca acttacgatg tgggtggatg gcgacaagca aatggcgttt
     3181 tataaaaaaa ttacggatca atatactaaa aaaactggca tcaaagtaaa gcttgtaaat
     3241 attggtcaaa atgatcaact agaaaatatt tcgctagacg ctcctgcagg aaaaggtcca
     3301 gatatctttt tcttagcaca tgataatact ggaagtgcct atctacaagg cttagctgct
     3361 gaaatcaaat tatcaaaaga tgagttgaaa ggtttcaata agcaagcact taaagcgatg
     3421 aattatgaca ataagcaact agcattgcca gctatcgttg aaacaaccgc acttttttat
     3481 aataaaaaat tagtgaaaaa tgcaccgcaa acgttagaag aagttgaagc taatgctgcc
     3541 aaactaactg atagtaaaaa gaaacaatac ggtatgttat ttgatgctaa aaatttctat
     3601 tttaattatc cgtttttatt cggcaatgat gattatattt tcaagaaaaa tggcagtgaa
     3661 tatgatattc atcagctagg actaaattca aaacgtgtcg tcaagaatgc tgaacgatta
     3721 caaaaatggt acgacaaagg gtatcttcct aaggcagcaa cacatgatgt catgattggt
     3781 ctttttaaag aaggaaaagt aggacaattt gtcactggac cgtggaacat taatgaatat
     3841 caagaaacgt ttggtaaaga tttaggagta acaacattac ctacagatgg tggcaaacct
     3901 atgaaaccat ttctaggtgt acgtggttgg tatttatctg aatatagtaa acataagtat
     3961 tgggctaaag atttaatgct gtatatcact agtaaagata cattacaaaa atatacagat
     4021 gaaatgagcg aaattactgg acgtgttgac gtgaaatcat ctaatccaaa tttaaaagtg
     4081 tttgaaaagc aagcacgtca tgctgaaccg atgcctaata ttcctgaaat gcgacaagtt
     4141 tgggaaccga tgggcaatgc aagcatattt atttcaaatg gtaagaatcc taaacaagcg
     4201 ttagatgagg cgacgaatga tataacgcaa aatattaaga ttcttcatcc atcacaaaat
     4261 gataagaaag gagattagtt atgacgaaac gtaaccctaa attagcggca ttattatctg
     4321 ttatacctgg tttgggacag ttttataata aaagacccat taaagggacg atatttttta
     4381 tctttttcat cagttttatt tctgtttttt atagcttttt aaatattggt ttttggggat
     4441 tgttcacatt agggacagta cctaagttag acgattctcg tgtcttactt gcacaaggta
     4501 ttatttctat cttactcgtt gctttcgcaa tcatgctata tatcattaat attttagatg
     4561 catatcgtaa tgctgaacga tttaatcgca atgaggaaat aaaggatccg aaggcgcgta
     4621 tggtggcaac atgggacaag acgttcccat acttactaat ctcaccaggt acattcttat
     4681 tgatatttgt agttgtattt ccattaatat ttatgtttgg agtagcattt acaaattaca
     4741 atttatacaa cgcgcctccg agacacacat tagaatgggt tggtttagat aactttaaaa
     4801 cgttattcac aattggcgtt tggcgtaaaa catttttcag tgttattact tggacattag
     4861 tatggacgct tgttgcaacg acacttcaaa ttgcattagg gctgtttttg gcaattattg
     4921 taaatcaccc tgtcgtcaaa ggtaagaaat ttatccgtac tgtgttaatc ctaccttggg
     4981 ctgtaccatc atttgtgaca attttaatat ttgtagcgtt atttaatgat gaatttggtg
     5041 cgataaataa tgatattttg caacctttat taggtgtagc accagcatgg ttaagtgatc
     5101 cgttttgggc aaaagtggca ttaatcggca ttcaagtatg gcttggattc ccatttgtct
     5161 ttgcactgtt cactggagta ctgcaaagta tttcatcaga ttggtacgaa gcagcagata
     5221 tggatggtgc gtctagttgg caaaagttta gaaacatcac attcccgcat gtcatttacg
     5281 ccacagcgcc attgttaatt atgcaatatg caggtaattt caataatttt aatcttattt
     5341 atctatttaa taaaggcggt ccaccagtgt cagggcagaa tgctggtagt acagatatct
     5401 tgatatcttg ggtgtataat ctgacatttg agtttaacaa cttcaacatg ggtgcagttg
     5461 tgtcattaat tattggattt attgttgcta ttgtcgcatt tattcaattc agacgtacaa
     5521 gtacgtttaa agatgaggga ggtttataag atgacaaaga agaaaaacat attaaaagca
     5581 atcggtattt acagttttat agcgatgatg tttgtcatca ttttatatcc actactgtgg
     5641 acatttggca tttcccttaa tccaggtacg aacttgtatg gtgccaaaat gataccagac
     5701 aatgcaacat ttaaaaatta tgcattctta ctattcgatg acagtagtca atacctgact
     5761 tggtataaaa atacgcttat cgtagcatct gcaaatgcac tgtttagtgt gatatttgtc
     5821 acgttaacag catatgcttt ttctagatat cgctttgttg gtcgtaaata cgggctgatt
     5881 acatttttga ttttacaaat gttccctgta ttaatggcaa tggtcgcaat ctatattttg
     5941 ctaaatacaa ttggattatt agattcttta tttggactaa cactggtata tattggtgga
     6001 tcaataccga tgaatgcctt tttagtgaaa ggttacttcg atacgattcc aaaagaactt
     6061 gatgaatctg ccaaaattga tggtgcaggg catatgcgta ttttcttaca aattatgctt
     6121 ccattagcta agccgatttt agcagttgtt gctttgttca attttatggg gccatttatg
     6181 gactttatat tacctaaaat actattaaga agtcctgaaa aattcacatt agcagttgga
     6241 ttgttcaact ttattaatga taagtatgca aataatttca cagtgtttgc agcaggggca
     6301 attatgattg cagtacctat agcaatcgta ttcttgttct tgcaacgcta tttagtatca
     6361 ggtttaacaa caggtgcgac aaaaggttag tttgaaatta ggagtggggc agaattgata
     6421 aagaaccact aatgacgata aagattaaaa ggaggacgtt atgatgacga ttaaagttgg
     6481 aatcattggg tgtggtggta ttgcgaatgg caagcacatg ccaagtttac aaaaagttga
     6541 aaatgttgaa atgatcgcat tttgtgacgt agacatttcg aaagcagcga gtgcggcaga
     6601 agcatacgga actgacaatg caaaggttta tgatgattac aaagcattgt taaaagatga
     6661 cacgattgat gttatccatg tttgtacgcc aaatgactcg cattgtgaaa ttactgtagc
     6721 agggttgcat gctggtaaac atgtgatgtg tgaaaaacca atggctaaaa cgacagcaga
     6781 agctcaaaaa atgatagata cagctaaatc aacaggtaaa aaattaacaa taggttatca
     6841 aaatcgtttc cgagcagata gtcaattttt acatcaagca gcgcaacgtg gcgacttagg
     6901 agacatttac ttcggaaagg cacatgccat tcgtcgtcga gcagtaccaa catggggtgt
     6961 ctttctagac gaagaagctc aaggtggagg accattaatc gatatcggta cacacgcttt
     7021 agatttaacg ttatggatga tggataatta tgaaccagaa tcagtgatgg gttcaacatt
     7081 ccataaatta aataaacagc atcatgcggc aaacgcttgg ggttcatgga atccagatga
     7141 atttacagtt gaagattctg cgtttggatt tattaaaatg aagaatggag cgacgatcat
     7201 gttagaatcc gcttgggcga ttaattcttt agaagtggat gaggcaaaat gttcattatc
     7261 aggaactaaa gcaggtgctg atatgaaaga tggtctacgt attcatggtg aagacatggg
     7321 tacactttat accaaacacg ttgaattgga aaacaaaggc gtcgactttt atgaaggtaa
     7381 tgaagtggat gaagctgaag aagaagcaaa agcttggatt gatgcagttg taaatgatac
     7441 tgaaccagtt gtgaaaccgg aacaagcaat ggtagttaca aaaattcttg aagcgattta
     7501 tcagtctgca aaatcaggca aagcaattta ctttgaataa catcatacgg taaggaggca
     7561 catcatgaca aaattaaaag ttggtgtgat aggtgttggt ggtattgcac aagaccgtca
     7621 tattccagca ttgctgaaac tcaaagacac agtctcatta gttgcagtac aagatattaa
     7681 tacagtgcag atgattgatg ttgcgaagcg ctttaatata cctcatgcag ttgagacacc
     7741 tagcgagctg tttaaacttg ttgatgcggt ggtcatttgt acacctaata aattccatgc
     7801 tgatctttct atagaagcat tgaaccatgg tgtccatgta ttgtgtgaaa agccaatggc
     7861 gatgacgacg gaagagtgtg atcgcatgat tgaagcggct aataaaaatc acaaattatt
     7921 aactgtcgca tatcattatc gtcacacaga tgtggcaatt actgctaaaa aagcaattga
     7981 atcaggtgtg gttggtaaac ctttagtagc acgtgtacaa gcgatgcgta ggcgtaaagt
     8041 gcctggctgg ggtgttttta ccaataaagc gttgcaaggt ggcggtagtt taatcgatta
     8101 tggttgccac ttgttagact tatctttgtg gctactaggt aaagatatgg tgccgcatga
     8161 agtgctagga aaaacatata atcaattgag caaacaaccg aatcaaatta atgattgggg
     8221 aacatttgat catactaaat ttgatgtcga tgatcatgtt actagttata tgacatttgc
     8281 caatcgagca agcatgcagt ttgaatgttc gtggtctgca aatatcaaag aagataaggt
     8341 tcacgttagt ttatcaggag aagatggcgg tatcaattta tttccatttg aaatatatga
     8401 gccccgcttt ggaactattt ttgaaagcaa agctaatgtt gagcataacg aagacattgc
     8461 tggtgagaga caggcgcgta actttgtcaa tgcgtgttta gggatagaag agattgtggt
     8521 gaaaccggaa gaagcacgca atgtaaatgc ccttatagaa gcgatttatc gtagcgatct
     8581 tgataacaag agcatacaac tttaatgatt atcatatatg atacaaaatt ctcaatataa
     8641 aaagaaggag tgcttttcaa tgaaaatagg tgtattttca gtattatttt acgataaaaa
     8701 ttttgaagat atgttagatt atgtctcaga atctggattg gatatgattg aagttggaac
     8761 aggtggtaac ccaggagata aattttgtaa gttagatgag ttgttagaaa atgaagacaa
     8821 gcgccaagca tttatgaagt caatcacaga cagaggctta caaataagtg gtttcagttg
     8881 tcataacaat ccaatttctc cagatccgat agaagcgaaa gaagccgatg aaacgttacg
     8941 taaaacaatc cgtttagcaa atctattaga cgtgccagtt gttaatacat tttctggcat
     9001 tgcaggatca gatgataccg ctaaaaagcc taattggcct gttacacctt ggccaacagc
     9061 ctactctgaa atttatgatt atcagtggaa tgaaaagttg ataccatatt ggcaagattt
     9121 agctgagttt gcaaaagagc aagatgtaaa aattgccata gagttgcatg caggattttt
     9181 agtgcataca ccatatacaa tgttgaagtt acgtgaggct acaaatgaat atatcggtgc
     9241 taacttagat cctagtcatc tatggtggca aggtattgac ccaattgctg cgattcgcat
     9301 attaggccaa gcaaatgcaa ttcatcactt ccatgctaaa gatacgtata ttaatcaaga
     9361 aaatgtaaat atgtatggtc taactgatat gcaaccatat ggtaacgttg cgacaagagc
     9421 atggacattc cgtacagttg gttatggaca tagtccatat gtatgggcag atatcataag
     9481 tcaacttatt attaatggat atgattatgt attaagtatt gaacatgaag atcctattat
     9541 gtcagtagaa gaaggtttcc aaaaagcttg tcaaactttg aaatctgtta atatttacga
     9601 caagccagca gacatgtggt gggcataata cgaactcgag gttagtctga agtttgtctg
     9661 aagtaagact ggtggcagtg ttgaataaat gcatatgtcg ccaagccatt gccaaaaatt
     9721 tcacacctta aatcatgtca ttgtttgtaa agaaggtgta ctttatataa gtgtttagcg
     9781 atggtcatac ccattcacag taacaatcct caccattgaa aagagtatat aaccttttcg
     9841 atagtgaggt atatgataat aaaaaaagcc tgttgtcaca atggtcatag acacgacata
     9901 ctttaaaggc ttctgaatat aatatttcag aacgcacttt aaagatggac gtcgatgtcg
     9961 actaatgtga tgacaggctt tcatcttttt aaatattcat taatttctct tcttgtttaa
    10021 tacgtacata taagaaatac gcatacggta ctaataaaat agttgtatat gttgcgtgtg
    10081 ttaataataa tacaccgatt aattcaggaa tgatgtttaa gaagtaattt gggtgttttg
    10141 taattttata taatccagat ttaataatag gatggttagg taaaatgaat aattttaatg
    10201 tccaaatacc acctaaagtt ttaataacca taaataacat gatataagca aagattaata
    10261 taactaagcc aataccattt gcaaagctaa atgtatcttt attaataaat gcctctacac
    10321 cagccaatac ataaattaaa acgtgtgtta ttgctaaaaa cttcgaattt ttaacgccat
    10381 attcaactgc accgtctgct tttaattgtt ttgagtgatt aatagatatc tttaagctga
    10441 caagtctgat acagaaaaag ataagtaata tagatagaat catgatgtcc tccgtcatta
    10501 tgtcata
//
LOCUS       DAMZKC010000094        10231 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.095, whole genome shotgun sequence.
ACCESSION   DAMZKC010000094 DAMZKC010000000
VERSION     DAMZKC010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10231
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.095"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(18..878)
                     /locus_tag="POB55_001741"
     CDS             complement(18..878)
                     /locus_tag="POB55_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI6500703.1"
                     /translation="MNLNILKSFLVTSETKNLTKASELLNYSQSTVSTHIEKLERQLD
                     VKLFYRKKYGMELTEEGLAYVKYAKVILDSNSEYEREIKGLYNKKVNISINMQESQYL
                     YRYYNKISEWLAEHPYVNLKFKSAHSNFYIKEEIANFKSDISLITDEKIINSNLTAIP
                     ITEERLIFVTNKEMKDFKLNDIQNHTLLVTEKGCSYREQLEMILYKYSFSTKQMIEFL
                     GIESLKKHLKNSGGIALLPEFIVADELENKSLFQIDVDVDIPNLETTLIINPESNKQV
                     LESFVKDVFL"
     gene            991..1542
                     /locus_tag="POB55_001742"
     CDS             991..1542
                     /locus_tag="POB55_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDI6500704.1"
                     /translation="MITCLFGSSRKDGNSAIAVENLLKNLKVNFVNLYQSNIEKVTDN
                     RHSKGANFHNDDYEQILIKVLESDIIIFSTPLYWYSMSASLKLFIDRWTESLRDTQID
                     NFKEIMSQKKYLILIIGGDNPHTKAQPLVNQFKLIFEFMNITNYRFLIGEGNKPFDIL
                     NDSQFIEELANINLALKKGDIYD"
     gene            1535..2755
                     /locus_tag="POB55_001743"
     CDS             1535..2755
                     /locus_tag="POB55_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6500705.1"
                     /translation="MTDLLKIKNFRLFFLAEIISAFGVGISTVGANWYLIDKTNDSQL
                     LGIMLALNVLSGFLASPIIGGLADKYNRRNIILITYLLQVILYLLIVIALVMIGFETY
                     LVIGFAIVNGIGWTTYMATSRSLVKQILKPDQYTDANSLLEISLQTGMFIAGGLSGIL
                     YKINGFTLIIAMTIMMFLISIFMLFRLHVDKPTHSEEESTNSLLQEYLLGWKFLKDNM
                     MIFIFGVISIIPMVFTMIFNISLPGYVYNVLKLSSVQFGFSDMLYGIGGLCAGLISAI
                     LSKKISTKVLIFLLYFILVINSALFIWINSAFYLFIGSFILGYSISSIRIYMNTAIMN
                     TVSDKYVGRSFTIWTSISLLLQSFIAPFLGRWINEINDKFGFYIILILSLLIFVTLLL
                     VNKTDKIKYAHKEE"
     gene            complement(2827..3123)
                     /locus_tag="POB55_001744"
     CDS             complement(2827..3123)
                     /locus_tag="POB55_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HDI6500706.1"
                     /translation="MATKKDVHDLFLNHVNSNAVKTRKMMGEYIIYYDGVVIGGLYDN
                     RLLVKATKSAQQKLQDNTLFSPYPGSKEMILILDFTEATNLTDLFKTIKNDLKK"
     gene            complement(3172..3375)
                     /locus_tag="POB55_001745"
     CDS             complement(3172..3375)
                     /locus_tag="POB55_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500707.1"
                     /translation="MEGLKHSLKSLGWWDLFFAIPIFLLFAYLPNYNFITIFLNIVII
                     IFFSIGLILTTHIIIDKIKSNTK"
     gene            3684..4670
                     /locus_tag="POB55_001746"
     CDS             3684..4670
                     /locus_tag="POB55_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HDI6500708.1"
                     /translation="MKENFWSELPRPFFILAPMEDVTDIVFRHVVSEAARPDVFFTEF
                     TNTESFCHPEGIHSVRGRLTFSEDEQPMVAHIWGDKPEQFRETSIQLAKMGFKGIDLN
                     MGCPVANVAKKGKGSGLILRPDVAAEIIQATKAGGLPVSVKTRLGYYEIDEWKDWLKH
                     VFEQDIANLSIHLRTRKEMSKVDAHWELIEAIKNLRDEIAPNTLLTINGDIPDRKTGL
                     ELAEKYGIDGVMIGRGIFHNPFAFEKEPREHTSKELLDLLRLHLSLFNKYEKDEIRQF
                     KSLRRFFKIYVRGIRGASELRHQLMNTQSIAEARALLDEFEAQMDEDVKIEL"
     gene            complement(5337..6530)
                     /gene="sqr"
                     /locus_tag="POB55_001747"
     CDS             complement(5337..6530)
                     /gene="sqr"
                     /locus_tag="POB55_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II sulfide:quinone oxidoreductase Sqr"
                     /protein_id="HDI6500709.1"
                     /translation="MNKHYQIVIIGGGTAGVTVASRLLRKNQNLKEKIAIIDPADHHY
                     YQPLWTLVGAGVSSLKSSRKDMESVIPEGANWIKQAVSSFQPENNSVILGDNTVVYYD
                     FLVVAPGLQINWSSIKGLKENIGKNGVCSNYSPDYVNETWNQISNFKQGNAIFTHPNT
                     PIKCGGAPMKIMYLAEDYFRKHKIRSNANVIYATPKDALFDVGKYNKELERIVEERNI
                     TVNYNYNLVEIDGDKKVATFEHIKAYDRKTISYDMLHVTPPMGPLDVVKESTLSDSEG
                     WVDVNPTTLQHKSYSNVFALGDASNVPTSKTGAAIRKQAPIVANNLLQVMNNQMLTHH
                     YDGYTSCPIVTGYNRLILAEFDYNKNTKETMPFNQAKERRSMYIFKKDLLPKMYWYGM
                     LKGLI"
     gene            complement(6549..7883)
                     /gene="cstB"
                     /locus_tag="POB55_001748"
     CDS             complement(6549..7883)
                     /gene="cstB"
                     /locus_tag="POB55_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="persulfide dioxygenase-sulfurtransferase CstB"
                     /protein_id="HDI6500710.1"
                     /translation="MFFKQFYDNHLSQASYLVGCQRTGEAIIIDPVRDLSKYIEVADS
                     EGLTITQATETHIHADFASGIRDVAKRLNANIYVSGEGEDALGYKNMPSKTQFVKHGD
                     IIQVGNVKLEVLHTPGHTPESISFLLTDLGGGSSVPMGLFSGDFIFVGDIGRPDLLEK
                     SVQIKGSTEISAKQMYESVQNIKNLPDYVQIWPGHGAGSPCGKALGAIPISTIGYEKI
                     NNWAFNEIDETKFIESLTSNQPAPPHHFAQMKQVNQFGMNLYQSYDVYPSLDNKRVAF
                     DLRSKEAFHGGHTKGTINIPYNKNFINQIGWYLDFEKDIDLIGDKSTVEKAKHTLQLI
                     GFDKVAGYRLPKSGISTQSVHSADMTGKEEHVLDVRNDEEWNNGHLDQAVNIPHGKLL
                     NENIPFNKEDKIYVHCQSGVRSSIAVGILESKGFENVVNIREGYQDFPESLK"
     gene            complement(7914..8981)
                     /gene="cstA"
                     /locus_tag="POB55_001749"
     CDS             complement(7914..8981)
                     /gene="cstA"
                     /locus_tag="POB55_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="persulfide response sulfurtransferase CstA"
                     /protein_id="HDI6500711.1"
                     /translation="MKQYGEKFIDEFSKAELEKLAKQGQLIDVRTEEEYALGHINGSI
                     LHPVDEIESFNKEKNKTYYVICRSGNRSANASKYLAKQGYNVINLDGGYKAYEEENDS
                     YDTQEEYKSIEIKADRKQFNYRGLQCPGPIVKISQEMKNIEVGDQIEVKVTDPGFPSD
                     IKSWVKQTRHTLVKLDENNNGINAIIQKEKAKDLDINYSAKGTTIVLFSGELDKAVAA
                     LIIANGARAAGKDVTIFFTFWGLNALKKVQTVNVKKQGIAKMFNLMLPKKNIRMPLSK
                     MNMFGLGNMMMRYVMKKKNVDSLPTLINQAIEQNIKLIACTMSMDVMGIQKEELRDEV
                     EYGGVGTYIGATENANHNLFI"
     gene            9118..9378
                     /gene="cstR"
                     /locus_tag="POB55_001750"
     CDS             9118..9378
                     /gene="cstR"
                     /locus_tag="POB55_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="persulfide-sensing transcriptional repressor
                     CstR"
                     /protein_id="HDI6500712.1"
                     /translation="MNYDKKMINRINRIQGQLNGIIKMMEEGKDCKDVITQISASKSS
                     LQRLMGIIISENLIECVKAAADDEESSQELINEAVNLLVKSK"
     gene            9378..10133
                     /locus_tag="POB55_001751"
     CDS             9378..10133
                     /locus_tag="POB55_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDI6500713.1"
                     /translation="MDIVNIVIMLLIGVFGGFISGLVGVGGAIIIYPAILLLPPLFGA
                     PAYSAYIASGLTSSQVFFSTLSGSLKARKKTEFSPQLVLYMGGGMIIGSMLGAFLANL
                     FDATFVNTVYIIIALLALTLMFIKVKPSSEKSSFNKYLLVIIGLFIGIISGIVGAGGA
                     FIIIPILLVLFKLPMNTVVANSIVIAFISSIGAFVIKLIQGYIPLYDALFLIIGSIIF
                     APIGLKLSKKVPNIIQKWIISILIVFAIIQLIL"
BASE COUNT         3333 a         1698 c         1516 g         3684 t
ORIGIN      
        1 cacgttttca ccaataatta taaaaaaaca tcttttacaa aagattcaag tacttgctta
       61 ttcgattcag gattaattat caatgttgtt tctaaattcg gaatatcgac atctacgtct
      121 atttgaaaca aacttttatt ttctaattca tctgcaacaa taaattccgg caatagcgca
      181 atcccacctg aatttttcaa gtgttttttt aatgactcaa ttcctagaaa ttcaatcatt
      241 tgttttgttg aaaagctata tttatataaa atcatttcta actgttctct gtaactacac
      301 cctttttcag taacaagtaa tgtatggttt tgaatatcgt ttaatttgaa atctttcatc
      361 tctttgttag taacaaatat taaacgttct tcagttatag gaatagcagt taagttacta
      421 ttaataatct tttcgtctgt gataaggcta atatccgatt tgaaattagc aatttcttct
      481 ttaatataga aattagaatg tgcggattta aacttcaagt ttacatatgg gtgttcagct
      541 aaccattcac taatcttatt atagtagcga tacaaatact gactttcttg catgttaata
      601 cttatattta ccttcttatt gtaaagtcct tttatttctc tctcgtattc gctattacta
      661 tctaaaatca ctttagcgta tttaacgtat gctaagcctt cttccgttag ttccatacca
      721 tatttttttc tataaaataa tttaacatct aattgccttt ctaatttttc aatatgtgta
      781 gatacagttg actgtgaata gttaagtaac tctgatgctt tagtaaggtt ctttgtttca
      841 ctagttacta aaaatgattt taaaatgttt aagttcaaag tagtacctcc tttagggttc
      901 ccttttatcg aatcctgaat tctaagaatt ctgatttaat aataatatat taaatattat
      961 tataaaggta aatcgaaagg agttatgact atgattactt gtttatttgg aagtagtaga
     1021 aaagatggca attcagctat cgcagtagag aatctattga agaatttaaa agtcaacttt
     1081 gtgaatttat atcaaagcaa cattgaaaag gttacagata atcgtcatag caaaggtgcc
     1141 aactttcata atgatgatta cgaacaaatt ttaatcaaag ttttagaatc agatattatt
     1201 attttttcaa caccactcta ttggtactcc atgagtgcgt ctttaaaact cttcattgat
     1261 agatggaccg agtcattgcg agatacgcaa atagacaact tcaaagaaat catgtcacaa
     1321 aaaaagtatt tgattttaat cataggtggc gacaatccgc ataccaaagc acaaccttta
     1381 gtcaaccaat tcaaattaat atttgagttt atgaatatta ctaactatag atttttgatc
     1441 ggcgaaggca ataaaccatt cgatatttta aacgactctc aatttataga agagctcgca
     1501 aatataaatc ttgcattgaa aaaaggtgat atttatgact gatttgttga aaattaaaaa
     1561 cttcagattg ttctttttag cagaaataat ttcagcattc ggagttggaa ttagtacagt
     1621 tggggctaat tggtatttaa tcgacaaaac taacgactcc caactattag gtattatgtt
     1681 agctttaaat gtattatccg gtttcttagc ttctcctata ataggtggac tagccgataa
     1741 atataataga cgaaatatta ttttaatcac ctatcttctt caggtaatat tatatttact
     1801 cattgtcata gcactcgtaa tgataggatt tgagacctat ttagtgattg gatttgcgat
     1861 tgttaatggg ataggttgga ctacgtatat ggcaacatct agaagtttag ttaaacagat
     1921 attaaaacca gatcaatata cagatgcaaa ttctctatta gagattagtt tgcaaacagg
     1981 tatgtttatt gcaggtggat tatcaggaat attatataaa ataaatggat tcactctaat
     2041 tatagcgatg actataatga tgtttctaat cagcattttt atgttattta gattgcatgt
     2101 agataaacca actcattcag aggaagaatc aacaaatagc ttattgcaag aatatttatt
     2161 aggatggaaa tttttaaaag acaacatgat gatatttatt tttggagtta tttcaatcat
     2221 accaatggtg tttacaatga tctttaacat atcattacca ggatatgttt ataatgtttt
     2281 aaaactttcg tctgtacaat ttggtttttc agatatgtta tatggcattg gaggattatg
     2341 tgcaggttta atttcagcta ttctttcgaa gaaaatctca actaaagtat tgatattttt
     2401 gttatatttc atattagtca taaattcagc actatttatt tggataaact cagcatttta
     2461 cttattcata ggatcattta tactaggcta ctcaatttca tcaatcagaa tttatatgaa
     2521 tacagctata atgaacactg tttcagataa atatgtcggt cgctcattta cgatatggac
     2581 gtcaatttca ttgttactgc aaagtttcat tgctccattt ttaggaagat ggattaatga
     2641 aattaatgat aaattcggtt tctatattat actcatttta tccttactca tatttgttac
     2701 actgctgctt gttaacaaaa cagacaaaat aaaatatgca cataaagaag agtgaccgtc
     2761 actcttcttt aacaagcgac catttatcga tgggcttagt tctctctgca cccacacttc
     2821 actacttcac tttttcaaat cattttttat ggtcttaaat aaatcagtga gatttgttgc
     2881 ttcggtaaag tctagaatta atatcatttc tttagaacct ggatatggcg aaaataatgt
     2941 attatcttgc aatttctgct gggcactttt agtcgccttg accaataatc tattatcata
     3001 caaaccacct ataaccacgc catcataata aataatatat tctcccatca tctttcttgt
     3061 cttaaccgcg tttgaattca catgatttaa aaataaatca tgtacatctt tcttagtcgc
     3121 cattgtaatc gctccttcag ttttatgttt aatcacattc gtattaatga ttcatttcgt
     3181 gttgctctta attttatcta taattatatg cgtagttaaa atcaaaccta tggaaaagaa
     3241 aataatgata acaatgttaa gaaatatagt tataaaatta tagtttggaa ggtatgcgaa
     3301 tagcagaaaa ataggtatcg caaaaaataa atcccaccaa cctaaacttt ttaaagaatg
     3361 ctttaaacct tccataatat cacctttata aatttgtctt tgttataaga taactaaaaa
     3421 atcgctttac tgtaaaagta gccaaagaaa attctgaatc atattcataa gtagtgtatc
     3481 attaataatg aacaatttaa tactataatc cttgatcttt gtattgatca acttaccaca
     3541 acatttattt tagactactc ttagacttcc ctttcaaatg gttgcatcta ttgaaattcc
     3601 ttttgtataa gttaggcttt tgtggtaata tcatcatgca taaaaaatcg agatactaat
     3661 tataaagagg gtataaatat attatgaaag aaaatttttg gagtgaatta ccacgtccat
     3721 tttttatttt ggcgccaatg gaagacgtta cagatatcgt ctttcgacac gttgtaagtg
     3781 aagcagctag accggatgtg tttttcactg aatttacaaa tactgaaagc ttttgccacc
     3841 ctgaaggcat acatagtgtg cgcggacgct taacttttag tgaagatgaa cagccgatgg
     3901 tcgctcatat atggggagat aagccagaac agttccgtga aacgagtatt caattagcta
     3961 aaatgggctt taaaggcata gacttaaata tgggatgtcc tgtagcaaat gttgctaaaa
     4021 agggtaaggg ttccggctta atcttaagac ctgacgttgc tgccgaaatt attcaagcga
     4081 ctaaagcagg tgggcttccg gtaagtgtta aaacacgcct tggctactat gaaatcgatg
     4141 aatggaaaga ttggttgaag cacgtcttcg aacaagacat tgccaattta tctattcatc
     4201 ttcgtacacg taaagaaatg agtaaagtag atgcacattg ggaattaatc gaagctatta
     4261 aaaatttacg tgacgaaatt gcaccaaata cattgttaac aattaacggt gatattcccg
     4321 atagaaaaac aggacttgaa ctggcagaaa aatatggcat tgatggcgtc atgattggta
     4381 gaggcatttt ccacaatcca ttcgcttttg aaaaagaacc acgtgaacac acaagcaaag
     4441 aactattaga tctgttgaga ttgcatctat cattgtttaa caaatatgag aaagatgaaa
     4501 tacgacaatt caagagcttg cgtagattct ttaaaatcta tgtgcgtggc ataagaggcg
     4561 ctagcgaact tcgccatcaa ttgatgaaca cacaatcaat tgcagaagca agagcacttc
     4621 tagatgaatt tgaagcccaa atggacgaag acgttaaaat tgaattatag tatgagtaat
     4681 aaagtttatg gatgatattt cccaatttaa cacggattga acactttcta cttaggtatt
     4741 atcttggttt tcctgatagg ataactcccg atgatgcctt tttacttatg tcacttatcc
     4801 ttttggaaat ggtgcaaaat taaaaataca tcaggatatc gtttaactca gaaaaaaact
     4861 ccaatcaaaa aaaatgttta caacacatta tgaaattaaa attgctctta gtgttcaaca
     4921 gacaaagtta ttgaaattac tattggaggt acataccaag ctatcatttt gagtacacgt
     4981 ggtttgaata tctggcaaga aatggaaatt ggtaatgtca gtcataaagt cgctaaacgt
     5041 gctcaaatac cattaattat ttttaaataa tttagaggat gaggtataaa cctttaaaaa
     5101 acagcagtga gatgattttc aattagaaaa tatcttactg ctgtttctat tttcataatc
     5161 attcttattg aatgacttca tcgtgatcat ttaaaacagt aacaactttt tcaatcttat
     5221 actatttaga gctaaaaatt cacgacatat aaacacaaca ctgacacgtc atttaaatac
     5281 ttaatctttt atagtaaaag atttttcatt aaaaatttat tgttttctgt actttattat
     5341 attaatcctt ttagcatgcc gtaccaatac attttaggta ataaatcttt cttaaatata
     5401 tacatacttc tacgttcttt ggcctgatta aacggcattg tttctttagt atttttatta
     5461 taatcaaact ctgcaagtat taacctatta tatccagtaa caatagggca tgaagtataa
     5521 ccatcataat gatgcgttaa catttgatta ttcatcactt gcaataaatt attagcgacg
     5581 ataggtgctt gcttacgaat agctgcgcct gtttttgaag taggtacatt tgaagcatca
     5641 ccaagtgcaa atacattaga gtagctttta tgctgtaatg tggttgggtt aacatctacc
     5701 caaccctcac tatctgaaag tgtactttct tttactacat ctaagggacc cataggtggt
     5761 gttacatgta acatatcata acttattgtt tttctatcgt atgctttgat atgttcgaat
     5821 gtagccactt ttttgtcacc gtcgatttca acaaggttat aattataatt gactgttata
     5881 tttctttctt caacaatcct ttctaattct ttattatatt ttcctacgtc aaataaagca
     5941 tcttttggcg ttgcatatat cacattagcg ttagaacgga ttttatgttt cctaaaataa
     6001 tcttcagcta aatacataat tttcataggc gcacctccac actttatagg agtgtttgga
     6061 tgcgtaaaaa tggcatttcc ttgtttaaaa ttagaaattt ggttccaagt ttcgttaaca
     6121 tagtcaggtg aatagttaga gcaaacacca tttttaccta tattttcttt tagtccttta
     6181 attgaagacc aattaatctg taatcctgga gctactacta aaaaatcata ataaacgact
     6241 gtattatctc ctaaaataac gctattattt tcaggttgaa aacttgaaac agcctgtttt
     6301 atccagttag caccttcagg tataacactt tccatatctt tacgagaact tttcaaacta
     6361 gatacccctg caccaaccaa cgtccataat ggttgatagt aatgatggtc tgctggatct
     6421 ataattgcta ttttctcttt taagttttga ttttttctta atagtcttga tgctacggta
     6481 acacctgctg taccgccacc aataataaca atttgataat gcttattcat tttttccaca
     6541 tccttaaatt attttaatga ttctggaaaa tcttgatagc cttctctaat attcaccaca
     6601 ttttcaaaac ctttgctttc caatataccc actgcaattg aacttctaac acctgactga
     6661 caatgtacat atattttatc ctctttatta aaaggaatat tttcatttaa taatttacca
     6721 tgcggaatat taactgcttg atctaagtgt ccattattcc actcttcatc attacgtacg
     6781 tctaatacat gttcttcttt acctgtcata tcagcgctat gaacggactg ggttgaaatg
     6841 cctgattttg gcaaacgata gcctgctacc ttatcaaacc caattaattg taaagtgtgt
     6901 ttcgctttct caacagtaga tttatctcca attaaatcta tatctttttc aaaatctaag
     6961 taccaaccaa tttgattaat aaagtttttg ttgtatggta tattgattgt tccttttgtg
     7021 tggccaccgt gaaaggcctc tttgctacga agatcaaatg ctactctctt attatctaaa
     7081 ctaggataaa catcatatga ttgatataaa ttcataccaa actgattaac ttgtttcatt
     7141 tgtgcaaaat gatgcggtgg tgctggttga tttgatgtta atgattcaat aaatttagtc
     7201 tcatcaattt cattaaatgc ccagttatta attttctcat aacctattgt agatataggt
     7261 atggcaccta atgctttacc acaagggctt ccagcaccat gacccggcca gatttgaaca
     7321 tagtctggta aatttttaat attttgaacg gactcataca tttgtttcgc gctaatttct
     7381 gtagaaccct ttatttgaac agatttttct aataaatcag gtctacctat atcaccaaca
     7441 aaaataaagt caccactaaa taatcccatc ggaacacttg aaccaccacc taaatcagtg
     7501 agtaaaaagc taatactttc aggcgtgtgt cctggagtat gcagaacttc taatttaaca
     7561 ttgcctactt gaatgatatc tccatgttta acaaattgtg tttttgatgg catattttta
     7621 taccctaatg catcttcacc ttcgccagac acatatatat ttgcatttaa gcgtttagcc
     7681 acatcacgaa ttcctgaagc aaaatcagca tgaatatgtg tttctgtagc ttgtgtaatt
     7741 gttaaacctt cagaatctgc aacttctata tatttcgata aatcacgaac agggtctatt
     7801 attattgcct ctcctgtacg ttgacaaccc actaaatatg atgcttgaga taaatgatta
     7861 tcgtaaaact gtttaaaaaa catgataaca actcctttta ttaatagatt taattagata
     7921 aataaattat gattcgcatt ttcagtagca ccaatataag tgcctacacc accgtactca
     7981 acttcatctc taagttcttc tttctgaata cccatgacat ccatactcat cgtacaagcg
     8041 attaatttga tattttgctc aatagcttga ttgataagtg ttggtaatga atcaacattt
     8101 ttctttttca ttacgtagcg catcatcata tttcctaaac caaacatatt cattttggaa
     8161 agaggcattc gtatattctt tttgggcaac attaaattaa acatttttgc aataccttgc
     8221 tttttaacat taactgtttg cacttttttt aatgcattaa gcccccaaaa agtaaagaag
     8281 atagttacat cttttccagc agctctagca ccatttgcaa taatcaacgc tgctacagct
     8341 ttgtctaatt ctccactaaa taatacaatt gtagtacctt tagcagaata atttatatct
     8401 aaatcttttg ctttttcttt ttgaataatc gcattaattc cattgttatt ttcatcaagc
     8461 ttaactaaag tatgccttgt ttgtttcacc caacttttaa tgtcactagg gaatccaggg
     8521 tctgtgactt tgacttcaat ttggtcacct acttcaatat tcttcatttc ttgactaatt
     8581 tttacaattg gccctggaca ttgaagacca cgatagttaa attgtttacg atctgcttta
     8641 atttctatac ttttatattc ttcttgtgta tcataactat cgttttcttc ttcataagct
     8701 ttataaccac catcaagatt tataacgtta taaccttgtt tagctaaata tttactagca
     8761 ttagcacttc tgttaccact tctacagatt acataatagg ttttattttt ttctttattg
     8821 aatgactcaa tctcatcaac aggatgaagt atggaaccat tgatatgtcc taatgcatac
     8881 tcctcttctg ttctaacgtc aattaattgc ccttgcttgg ctagtttttc caattctgct
     8941 ttactaaatt catcgataaa cttttctccg tattgtttca taataacctc cctaaaaata
     9001 cctgtagggg tattttaaac agttggatgg aacttgtcaa atacctctag gggtatttga
     9061 cacatttgtg tttatctatt aatatatttt tattcaatta aaagtggagg tatgactatg
     9121 aattatgata aaaaaatgat taatcgtatt aatagaatac aagggcaact aaatggaatt
     9181 attaaaatga tggaggaagg aaaagactgt aaagatgtca ttacacaaat aagtgcatca
     9241 aagagttcac tccaacgctt gatgggtatc attattagtg agaatttaat agaatgtgta
     9301 aaagcagctg cggatgatga agaaagctcc caagagttaa ttaatgaagc tgtaaactta
     9361 ttggtgaaaa gtaagtaatg gatattgtta atatagttat catgttactc attggggtat
     9421 tcggtgggtt catatctggt ctagtaggcg taggtggtgc tatcattatt tatccagcta
     9481 ttttattatt accaccatta ttcggagccc ctgcatatag cgcatatatc gcatcaggtc
     9541 ttacttctag ccaagtcttt ttcagtacat taagtggctc attaaaagct cgaaaaaaaa
     9601 cagagttctc gccacaatta gttctttata tgggtggcgg catgattata gggagtatgt
     9661 taggtgcatt tctagcaaat ttatttgatg ctacatttgt aaatacggta tatatcatta
     9721 ttgctttact tgcattaaca ttaatgttta ttaaagtgaa acctagttcc gaaaagtctt
     9781 catttaataa atatttatta gttatcattg gccttttcat tggtattatt tcaggtatag
     9841 taggtgcagg tggtgccttc attattattc ctatattatt ggttttattc aaattaccaa
     9901 tgaatactgt agtcgctaat agtatagtaa ttgcttttat atcttcaatt ggtgcttttg
     9961 tcatcaaact tattcaaggt tatatccctt tatatgacgc cttattcctt ataataggga
    10021 gtattatatt tgctccaata gggttaaaat taagtaaaaa agtccctaac ataatacaaa
    10081 aatggattat cagtatttta atcgtttttg ctataattca actcatttta taaaactaat
    10141 ccttttaact cagacagcaa tttcccgaaa aataatacct cttatttaaa caaatatgta
    10201 tgcagttcgg atttatataa ataaaaattt t
//
LOCUS       DAMZKC010000095        10128 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.096, whole genome shotgun sequence.
ACCESSION   DAMZKC010000095 DAMZKC010000000
VERSION     DAMZKC010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10128
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.096"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(33..1091)
                     /gene="hisC"
                     /locus_tag="POB55_001752"
     CDS             complement(33..1091)
                     /gene="hisC"
                     /locus_tag="POB55_001752"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HDI6500714.1"
                     /translation="MKEQLNQLSAYQPGLSPRALKEKYGIEGDLYKLASNENLYGPSP
                     KVKEAISAHLDELYYYPETGSPTLKAAISKHLNVDQSRILFGAGLDEVILMISRAVLT
                     PGDTIVTSEATFGQYYHNAIVESANVIQVPLKDGGFDLEGILKEVNEDTSLVWLCNPN
                     NPTGTYFNHESLDSFLSQVPPHVPVIIDEAYFEFVTEEDYPDTLALQQKYDNAFLLRT
                     FSKAYGLAGLRVGYVVASEHAIEKWNIIRPPFNVTRISEYAAVAALEDQQYLKEVTHK
                     NSVERERFYQLPQSEYFLPSQTNFIFVKTKRVNELYEALLNVGCITRPFPTGVRITIG
                     FKEQNDKMLEVLSNFKYE"
     gene            complement(1331..2845)
                     /locus_tag="POB55_001753"
     CDS             complement(1331..2845)
                     /locus_tag="POB55_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HDI6500715.1"
                     /translation="MTNFFDILSERKGQLFSTMIEHIQISFIALLIATAIAVPLGILL
                     TKTKTISEIVMNIAAILQTIPSLALLGLMIPLFGIGRVPAIIALVVYALLPILRNTYT
                     GIKEVDPSLIEAAKGIGMKPFRRLTKVELPIAMPVIMAGVRTAMVLIIGTATLAALIG
                     AGGLGDLILLGIDRNNASLILLGAIPAALLAIIFDLILRFMAKLSYKKLLMTLGVIVM
                     IIILAIAIPMFAQKGDKITLAGKLGSEPSIITNMYKILIEEETKNTVEVKDGMGKTAF
                     LFNALKSDDIDGYLEFTGTVLGELTKEPLKSKEEKKVYEQAKQSLEKKYQMTMLKPMK
                     YNNTYALAVKRDFAKQHNIRTIGDLNKVKDQLKPGFTLEFNDRPDGYKAVQKAYNLNL
                     DNIRTMEPKLRYQAINKGNINLIDAYSTDAELKQYDMVVLKDDKHVFPPYQGAPLFKE
                     SFLKKHPEIKKPLNKLENKISDEDMQMMNYKVTVKNEDPYTVAKDYLKAKGLIK"
     gene            complement(2838..3815)
                     /locus_tag="POB55_001754"
     CDS             complement(2838..3815)
                     /locus_tag="POB55_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6500716.1"
                     /translation="MIKFKNVTKRYGKHVAVDNISFNINEGEFFVLIGPSGCGKTTTL
                     KMINRLIHLSEGYIYFKDKPISDYPVYEMRWDIGYVLQQIALFPHMTIKENIAQVPQM
                     KKWKEKDIDKRVDELLEMVGLEPEKYKNRKPDELSGGQRQRVGVIRALAADPPVILMD
                     EPFSALDPISREKLQDDLIELQTKIKKTIIFVTHDIQEAMKLGDKICLLNEGHIEQID
                     TPEGFKNNPQSEFVKQFMGSHLEDVAPCVEENAIIRDLDIMKPIDEVTSMSAYPIVYD
                     NQPIEVLYQLLSESERVIVMQEDSVGQYVIDRKDIFKYLSQKKEVAQHD"
     gene            complement(4037..5818)
                     /gene="recQ"
                     /locus_tag="POB55_001755"
     CDS             complement(4037..5818)
                     /gene="recQ"
                     /locus_tag="POB55_001755"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HDI6500717.1"
                     /translation="MMQQTLSHYFGYETFRPGQEEIISKVLDHRNVLGVLPTGGGKSI
                     CYQVPGLLLGGTTIVISPLISLMKDQVDQLKAMGIQAAFLNSSLTQKEQQRIEKALSN
                     GEIQFLYVAPERFENRYFLNMLQRIKIHLVAFDEAHCISKWGHDFRPSYQNVISKVFT
                     LPQDFTIIALTATATVEVQQDIREKLNIAQTDQIKTSTKRRNLIFKVNPTYQRQKFIL
                     DYIKTHDEDAGIIYCSTRKQVEELQEALESQKIESVIYHAGLSNKEREEAQNDFLFDR
                     VKVVVATNAFGMGIDKSNVRFVIHYNMPGDLESYYQEAGRAGRDGLKSECILLFSERD
                     INLHEYFITVSQADDDYKDKMGEKLTKMIQYTKTKKCLEATIVHYFEPNEKLEECEQC
                     SNCVQQDKSYNMTQEAKMIISCIARMKQQESYSVIIQVLRGESTDYIKYKGYDQISTH
                     GLMKGYTTSELSHLIDELRFKGFLNENDEILMCDTSIKKLLSNEVEVFTTPFKQKATE
                     KVFINTVEGVDRVLFSQLVEVRKKLSDKLTIAPVSIFSDYTLEEFAKRKPASKQDMIN
                     IDGVGSYKLKHYCPAFLETIQNYKAKV"
     gene            complement(5830..7713)
                     /locus_tag="POB55_001756"
     CDS             complement(5830..7713)
                     /locus_tag="POB55_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDI6500718.1"
                     /translation="MGMEAYKIEHLNKSYADKTIFDNLDLSISEGEKIGLVGINGTGK
                     STLLKVIGGIDDDFTANVMHPNQYRIRYSSQKQDLNEDMTVFDAVLSSDTTTLRIIKQ
                     YEQAVQAYADDQSDKLFKRMMDAQDAMDQHDAWDYNAEIKTILSKLGIHDTTKYIKEL
                     SGGQQKRVVLAKTLIEQPDLLLLDEPTNHLDFESISWLINYVKQYPHTVLFVTHDRYF
                     LNEVSTRIIELNRGKLASYPGNYESYIEMRAEREVSLQKQQQKQRALYKEELAWMRAG
                     AKARTTKQQARINRFNDLENEVNQQYKDDKGELNLAYSRLGKQVFELEDLSKAINDKV
                     LFEHLTEIIQKGERIGVVGPNGAGKTTLLNILSGEDQQFEGKLKTGQTVKVAYFKQTD
                     ETLDRDIRMIDYLREESEIAKEKDGTSVSITQLLERFLFPSATHGKKVYKLSGGEQKR
                     LYLLRLLVHQPNVLLLDEPTNDLDTETLTILEDYIHTFGGTVITVSHDRYFLNKVAQS
                     YWFIHDGQMEKIIGTFEDYESYKKSLDKNKSTLKQQSKSSTTVRKKNGLSYKEKLEYE
                     QLMKRIEQAEVRMEEIDVLMIEASVDYGKIKELNEEKEQLEIQYDLDITRWSELEEIK
                     EQQ"
     gene            complement(7984..9924)
                     /gene="ltaS"
                     /locus_tag="POB55_001757"
     CDS             complement(7984..9924)
                     /gene="ltaS"
                     /locus_tag="POB55_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyglycerol-phosphate lipoteichoic acid
                     synthase LtaS"
                     /protein_id="HDI6500719.1"
                     /translation="MSSQKKKISLFAFFLLTVITITLKTYFSYYVDFSLGVKGLVQNL
                     ILLMNPYSLVALVLSVFLFFKGKKAFWFMFIGGFLLTFLLYANVVYFRFFSDFLTFST
                     LNQVGNVESMGGAVSASFKWYDFVYFIDTLVYLFILIFKTKWLDTKAFSKKFVPVVMA
                     ASVALFFLNLAFAETDRPELLTRTFDHKYLVKYLGPYNFTVYDGVKTIENNQQKALAS
                     EDDLTKVLNYTKQRQTEPNPEYYGVAKKKNIIKIHLESFQTFLINKKVNGKEVTPFLN
                     KLSSGKEQFTYFPNFFHQTGQGKTSDSEFTMDNSLYGLPQGSAFSLKGDNTYQSLPAI
                     LDQKQGYKSDVMHGDYKTFWNRDQVYKHFGIDKFYDATYYDMSDKNVVNLGLKDKIFF
                     KDSANYQAKMKSPFYSHLITLTNHYPFTLDEKDATIEKSNTGDATVDGYIQTARYLDE
                     ALEEYINDLKKKGLYDNSVIMIYGDHYGISENHNNAMEKLLGEKITPAKFTDLNRTGF
                     WIKIPGKSGGINNEYAGQVDVMPTILHLAGIDTKNYLMFGTDLFSKGHNQVVPFRNGD
                     FITKDYKYVNGKIYSNKNNELITTQPADFEKNKKQVEKDLEMSDNVLNGDLFRFYKNP
                     DFKKVNPSKYKYETGPKANSKK"
BASE COUNT         3116 a         1841 c         1378 g         3793 t
ORIGIN      
        1 agaacattga tttatgtccc agcctgcact tactattcgt atttaaagtt tgataaaact
       61 tctaacattt tatcattttg ttctttaaaa ccaattgtaa ttctaacacc agttggaaat
      121 ggtcgcgtaa tacaccctac atttaaaagt gcttcataaa gttcatttac ccgctttgtt
      181 tttacaaata taaaattcgt ttgacttggc aagaaatact cactttgagg taattgataa
      241 aatctttcgc gttcaacact atttttatgt gttacctctt ttaaatattg ttgatcttca
      301 agtgctgcaa ctgctgcgta ttcagatata cgtgtcacat taaatggtgg tctaatgatg
      361 ttccattttt caatcgcatg ttcacttgct accacatatc ctacacgtaa acccgctaat
      421 ccatacgcct ttgaaaatgt acgtaataag aaagcattgt catatttttg ttgcaaagca
      481 agtgtatccg ggtagtcctc ttctgtcaca aattcaaaat aagcttcatc tataattact
      541 ggtacatgtg gaggtacttg agataaaaac gaatctaagc tctcatgatt aaaatatgta
      601 cctgtaggat tatttggatt acataaccat accaatgacg tatcttcatt aacttctttt
      661 aaaatacctt ctaaatcgaa gccaccatct tttaaaggta cttgtatcac attagctgat
      721 tcaacaatcg cattgtgata atattgaccg aatgtcgctt cacttgtaac aatagtatcc
      781 cctggcgtta atacagctct agaaatcatt aatataactt catctaatcc cgcaccaaat
      841 aaaatgcgtg attgatctac atttaaatgt ttactaatcg ccgcttttaa tgtcggtgat
      901 cctgtttcag gataataata taactcatct aagtgtgctg atatcgcttc tttaacttta
      961 ggcgatggtc catacaaatt ttcatttgat gcaagtttat ataaatctcc ttcaatgcca
     1021 tacttttctt tcaatgccct tggagataaa ccaggctgat atgctgatag ttgattaagt
     1081 tgttctttca taataaaaac aacctcctta gaattaactg aaattttaaa aaacttatat
     1141 tcattatatc gctctatgtt tctaaaatgc aatgctacta ttttcacatc ataatagctt
     1201 acattgtgtt taacatgtca tattttttaa tcattattgc atgtttaaat tgaacataat
     1261 tatagattta aatataacaa cgccacataa gatataattt ggtgttacgc atcttatgtg
     1321 gcgttggtcg ttatttgatt aacccttttg cttttaaata atctttcgca actgtatatg
     1381 ggtcttcatt tttaactgtt actttatagt tcatcatttg catatcttca tcagatattt
     1441 tgttttctag tttgtttaac ggtttcttaa tttctggatg tttctttaaa aagctttctt
     1501 taaataatgg tgctccttga tatggtggaa atacgtgctt atcatctttt aacacaacca
     1561 tatcatattg ttttaattca gcgtcagttg aatatgcatc tattaaatta atattacctt
     1621 tattgatcgc ttgatatctc aacttaggtt ccattgtacg tatgttatct aaatttaaat
     1681 tataagcctt ttgaacagct ttgtaaccat ctggacgatc attaaattcc aatgtaaatc
     1741 ctggtttaag ttgatcttta accttattta aatcaccaat tgtacgtata ttatgttgtt
     1801 tagcaaaatc acgttttaca gctaaagcat acgtattgtt atacttcatt ggttttaaca
     1861 tagtcatttg atatttcttt tcaagacttt gcttagcttg ttcataaact tttttctctt
     1921 cttttgactt caatggttct tttgttaatt cgcctaaaac tgttccagta aattctaaat
     1981 acccatctat atcgtcagat tttaaagcat taaataaaaa tgctgttttg cccataccat
     2041 ctttcacttc tacagtattt ttggtctctt cttctattaa aattttatac atatttgtaa
     2101 taatcgatgg ctcggagcca agctttccag ctaacgtaat tttatcacct ttttgtgcaa
     2161 acataggaat agcgatagcc agtataataa tcatcactat aacacctaac gtcatcaata
     2221 actttttata agataattta gccataaatc ttaaaattaa atcaaatata attgccaata
     2281 aggctgctgg aattgcacct aataatatca acgatgcatt gttacggtct atacctaata
     2341 aaattaaatc tcctagtccg cctgcaccaa ttaatgctgc tagtgttgct gtacctataa
     2401 ttaataccat agccgttctt acaccagcca ttataacagg cattgctatc ggaagttcga
     2461 ctttagttaa acgtctaaat ggtttcatac ctataccttt agccgcttca atgagtgatg
     2521 gatcaacttc tttaattcca gtatacgtat tccttaaaat tggtaacaac gcatacacta
     2581 caagtgcaat aattgctggc acacgaccga taccaaataa aggaatcatt aaacctaata
     2641 atgccaacga tggtatggtt tgaagaattg ccgcaatatt cattacgatt tcagatatcg
     2701 ttttagtctt cgttaataaa atacctaatg gtaccgcaat agcagttgca atcaataatg
     2761 cgataaatga tatttgaata tgttctatca ttgtcgaaaa gagttgcccc ttacgttcac
     2821 tcaatatgtc gaaaaagtta gtcatgttga gctacctcct ttttctggga caaatatttg
     2881 aagatatctt tcctatcaat aacatattga cctacgctat cttcttgcat gacaatgaca
     2941 cgctcgctct ctgataaaag ttgatacaat acttcaattg gttgattgtc ataaacaatt
     3001 ggataagcgc tcatagatgt aacctcatcg attggtttca taatatccaa gtcacggata
     3061 attgcgttct cttcaacaca tggcgcaaca tcttctaaat gactacccat aaattgttta
     3121 acaaattcac tttgaggatt atttttaaat ccttctggtg tgtcaatttg ttcaatatgc
     3181 ccttcattca aaagacaaat cttatcacca agtttcatcg cctcttgaat atcatgtgta
     3241 acaaatatga ttgtcttctt aattttagtt tgtaattcaa ttaaatcatc ttgaagtttt
     3301 tctcggctga ttgggtctaa tgcactaaac ggttcatcca ttaaaataac tggtggatca
     3361 gctgctaacg cacgtataac tcctacacgt tgtcgttgcc cccctgacaa ttcatcaggt
     3421 tttctgtttt tatatttttc aggttctaat ccaaccattt caagtaattc atctactctt
     3481 ttatctatat ctttttcttt ccactttttc atttgtggca cttgtgcaat attttctttg
     3541 attgtcatat gtgggaataa tgcaatctgc tgcaatacgt atccaatatc ccaacgcatt
     3601 tcgtatactg gataatcact tattggttta tctttaaaat aaatataacc ttcacttaag
     3661 tgaatgagtc gattaatcat ttttaatgtc gtagtttttc cacaacctga aggtccaatt
     3721 agcacaaaaa attcaccctc attaatattg aaactaatgt tatcgacagc aacatgtttg
     3781 ccataacgct tagttacatt tttaaactta atcactttgc cacctctttt tttctcatag
     3841 cataaaaccg agattatatg tatgtattcc ctatttaacc acgtttatta caattttcaa
     3901 atttaaatga tttatccttg aactttttta ataaaataat gaataatagg taatctccag
     3961 ttaagaaata gtgttatttt accttgaatt caaaaaaaca cccagtaaaa caaggaatgc
     4021 ttactaggtg tcttcactat actttggctt tataattttg aatcgtttct aaaaatgctg
     4081 gacaataatg ttttaatttg taactaccta cgccatcaat attaatcata tcttgtttcg
     4141 aagcaggctt acgtttagca aattcctcca acgtgtaatc agaaaatata cttacaggtg
     4201 ctatcgttaa tttgtcactt aactttttac gaacttctac caactgactg aataatactc
     4261 ggtcaacccc ttcaaccgta tttataaata ctttttcagt cgctttttgc ttaaatggtg
     4321 ttgtgaatac ttctacttca ttactgagta attttttaat tgaagtatca cacattaata
     4381 tttcatcatt ttcatttaag aaccctttga atcttaattc atctattaag tgacttaatt
     4441 ctgatgttgt gtaacctttc attaaaccat gggttgaaat ttgatcataa cctttgtact
     4501 taatataatc tgttgactct cctcttagca cttgaatgat aacactataa ctctcttgtt
     4561 gtttcatacg agcgatgcaa ctaataatca tcttagcttc ttgtgtcata ttatatgatt
     4621 tatcttgttg aacacaatta ctacattgtt cacattcttc taatttttca ttcggttcaa
     4681 aataatggac aattgttgct tctaaacatt tttttgtttt tgtatattga atcattttag
     4741 ttaacttttc gcccatttta tctttatagt catcatcagc ttgagagact gttataaaat
     4801 actcgtgtaa attgatatcg cgttcgctaa ataacaaaat acattcactt ttcaacccgt
     4861 cacgacctgc acgaccggct tcttgataat aagattctaa atctccaggc atattataat
     4921 gaataacaaa acgtacattg gatttatcaa tacccatacc aaaagcattt gtagcaacga
     4981 ctactttaac acgatcaaat aagaaatcat tctgcgcttc ttctctttct ttattgctca
     5041 aacctgcatg atatataaca ctttcaattt tctggctttc taaggcttct tgaagctctt
     5101 caacttgctt acgtgtagaa caataaataa tacctgcatc ttcatcatgt gttttaatat
     5161 aatccaatat aaatttttga cgttgataag taggatttac tttaaaaatt aagtttctac
     5221 gcttagtact cgttttaatt tgatcagttt gagcgatatt taacttttct ctaatatctt
     5281 gctgtacttc aaccgtggca gttgctgtca acgctattat tgtaaaatct tgaggtaacg
     5341 taaatacttt tgaaataaca ttttggtaac tcggcctgaa atcatgaccc catttagaaa
     5401 tacaatgcgc ttcatcaaac gcgactaagt gaatctttat acgctgaagc atatttaaaa
     5461 aatatcggtt ttcaaatcgt tctggtgcaa catacaaaaa ttgaatttct ccatttgata
     5521 atgctttttc aatacgttgt tgctcttttt gagtcaaact actatttaaa aaagcagctt
     5581 gaattcccat cgcttttaat tgatccactt gatctttcat taatgatatt agtggactta
     5641 ttacaattgt tgtaccacct aacaataaac ctggtacttg atagcatata gacttacctc
     5701 caccagttgg taagacacca agcacattac gatggtctaa tactttgcta ataatttctt
     5761 cttgtcctgg tcgaaacgtt tcatacccaa agtaatgcga taatgtttgt tgcatcataa
     5821 attgacccct tattgttgtt ctttaatttc ttctaactca ctccatcttg tgatgtctaa
     5881 atcatattga atttcaagtt gttctttttc ttcgtttaat tctttaattt tcccataatc
     5941 tacacttgcc tcaatcatga gcacatctat ttcttccatt cttacttccg cttgttctat
     6001 gcgtttcatc aattgttcat attctaactt ttctttatat gataaaccat ttttcttacg
     6061 tacagttgta gaagatttag attgttgctt caatgtggat ttatttttat ctaatgattt
     6121 tttataactt tcataatctt caaaagttcc gataatcttt tccatctgac catcatgaat
     6181 aaaccaatat gactgtgcaa ctttatttaa aaagtagcga tcatggctta cggtaatcac
     6241 tgtaccaccg aaagtatgaa tataatcttc aagtattgtt aaagtctcag tatctaaatc
     6301 atttgtcggt tcatctaaca acagaacatt tggctggtgt acgagtagac gtaataaata
     6361 caaacgcttt tgctctccac cagataattt ataaactttt ttaccatgag ttgcacttgg
     6421 aaataaaaat cgttcaagaa gttgtgtaat agataccgag gttccatctt tttcttttgc
     6481 gatctcactt tcttctctta aataatcaat catacgaata tctctatcca gggtctcatc
     6541 tgtttgctta aaataagcta ctttaaccgt ctgtccagtc ttcaatttac cttcgaattg
     6601 ttggtcttct ccactcaaaa tatttaagag tgttgtttta ccagctccat ttggcccaac
     6661 aacaccaata cgctcacctt tttgaataat ttccgtcaga tgttcaaata atactttatc
     6721 attaatagcc tttgataagt cttctaattc gaacacttgc ttacctaatc ttgaataagc
     6781 aagattcaat tcacctttat cgtctttata ttgctggtta acttcatttt ctaggtcatt
     6841 aaatcgatta attctagctt gttgctttgt agtacgagcc ttagctccag ccctcatcca
     6901 agcaagttct tccttatata aagctcgttg cttttgttgt tgcttttgaa gtgatacttc
     6961 tctttcagcg cgcatttcaa tataagattc atagttacca ggatatgacg ctaacttacc
     7021 tctgtttagt tcaataattc tagtggaaac ttcatttaaa aaatatcgat catgggttac
     7081 gaataaaaca gtatgaggat attgcttcac ataattgatc aaccagctga ttgattcgaa
     7141 gtctaaatgg ttcgtaggtt catctaataa caataaatct ggttgttcta ttaatgtttt
     7201 tgcaagtaca acacgttttt gttgtccgcc ggataattct ttaatgtatt tagtagtatc
     7261 atgtatacct agttttgaga ggattgtttt aatttcagcg ttatagtccc aagcatcatg
     7321 ttgatccata gcatcttgcg catccatcat tcgcttgaac aatttatcac tttggtcatc
     7381 cgcataagct tgtactgcct gctcatattg cttgatgatg cgtaaagttg ttgtatcaga
     7441 acttaatact gcatcaaaaa ctgtcatatc ttcattaagg tcctgtttct gagacgaata
     7501 tcgaattcga tattgatttg gatgcataac attggctgta aaatcatcat caataccacc
     7561 aattactttt aacaacgtac ttttccctgt accatttatg cctactaaac ctattttttc
     7621 accttctgaa attgataaat ctaggttatc gaatatagtc ttatcggcat aagatttatt
     7681 taaatgttca attttatatg cttccatacc catcttcctt cttctgttga aatctctacg
     7741 tacttaaatc agttctatac attatacacc tttttgaaat caaaagaaat cgataccgtt
     7801 aagtgtcata atttaatata ttgttgcttc aactataaat aaaacttgtt aaggctttga
     7861 tgactatgct aatgatcttt ataatatacc tttttatcaa cacatataat gtaaaacata
     7921 atataaaaga acataataaa aacccggatt gatattttat caatccgagt tcgtgtttaa
     7981 atattatttt ttagagtttg ctttaggtcc tgtttcatat ttatacttcg aaggatttac
     8041 ctttttgaag tctggatttt tgtagaatct aaacaaatca ccattaagca cgttgtcact
     8101 catttcgaga tccttttcaa cttgcttttt attcttttcg aaatcagctg gttgagtagt
     8161 tatgagttca ttatttttat tagaataaat cttaccatta acatatttat aatcttttgt
     8221 tataaagtca ccatttctga atggaactac ttgattatga cctttagaga ataaatcagt
     8281 accgaacatt aaataattct tcgtatctat accagccaaa tgtaaaattg ttggcattac
     8341 atcgacttga ccagcatatt cattattgat accaccagat ttaccaggga ttttaatcca
     8401 gaaaccagtt ctgtttaaat ctgtaaattt agctggtgtg attttttcac ctaatagttt
     8461 ttccatggca ttgttatggt tttcagagat accatagtgg tcaccataaa tcataatcac
     8521 tgaattgtca tataatcctt ttttcttcaa gtcattaata tattcttcta atgcttcgtc
     8581 taaataacgc gctgtttgaa tataaccatc aactgttgca tcacctgtgt ttgacttctc
     8641 aatagtcgca tccttttcat ctaatgtgaa tggatagtgg ttagtcaaag taattaaatg
     8701 agaatagaat ggtgatttca tcttagcttg ataattagca gaatctttaa agaaaatttt
     8761 gtctttcaag cctaagttta caacgttttt atcagacatg tcatagtatg ttgcatcata
     8821 gaatttatcg ataccaaagt gtttatatac ttggtctctg ttccagaatg ttttatagtc
     8881 accgtgcatg acatcagatt tgtagccttg cttttgatct aaaattgctg gtagtgactg
     8941 atacgtatta tctcctttta atgaaaaggc agaaccttgc ggtaaaccgt ataaactgtt
     9001 atccattgta aattcagagt cagatgtttt accttgacct gtttgatgga aaaagttagg
     9061 gaagtatgtg aattgctctt tcccacttga taatttgttt aaaaacggtg ttacttcttt
     9121 accattaacc tttttattaa ttaagaaggt ttggaaactt tctaaatgaa tcttaataat
     9181 atttttcttc tttgccaccc cataatattc tgggttaggc tctgtttgac gttgtttcgt
     9241 ataatttaat acttttgtta agtcatcttc agatgctaac gctttttgtt gattattttc
     9301 gatagtttta acaccatcgt atactgtaaa gttatacggt cctaaatatt tcactaaata
     9361 tttatggtca aatgtacgtg ttaataattc tggtctgtca gtttcagcaa aagctaagtt
     9421 taagaagaat aatgctactg aagccgccat tacgacagga acaaatttct tactaaatgc
     9481 ttttgtgtct aaccattttg ttttaaatat taaaatgaat aagtaaacta acgtatcaat
     9541 gaaataaaca aagtcatacc atttgaatga tgcactaacc gcaccaccca tagattctac
     9601 gttacctact tggtttaaag tactaaacgt taaaaaatcc gagaagaatc taaagtacac
     9661 aacattggca tataatagga acgtcaataa gaagccgcct atgaacatga accaaaatgc
     9721 ttttttgcct ttaaagaata ggaacacact taaaaccagt gctactaaac tataaggatt
     9781 catcaataat attaagtttt gtactaaacc tttaacacct aaagaaaaat caacataata
     9841 agaaaaatac gtcttcaagg taatcgttat tacggttaat aagaagaacg caaaaagact
     9901 aattttcttt ttttgtgaac tcatgattct ttcccccgtt atttagataa taaatcttgc
     9961 gaaagtactt gaataaatta gatattaaca ttatgtagtt aattaataag tgcgtttaaa
    10021 agttaacatt gtcataatta tagtaattac tttcagattc aagtttataa ttttcattat
    10081 ttgtagcttt caactttgat ttaacaagta ttttgggcta caactcat
//
LOCUS       DAMZKC010000096        10040 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.097, whole genome shotgun sequence.
ACCESSION   DAMZKC010000096 DAMZKC010000000
VERSION     DAMZKC010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10040
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.097"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            188..1672
                     /locus_tag="POB55_001758"
     CDS             188..1672
                     /locus_tag="POB55_001758"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HDI6500720.1"
                     /translation="MDASTLFKKVKVKRVLGSLEQQIDDITTDSRTAREGSIFVASVG
                     YTVDSHKFCQSVADQGCKLVVVNKEQSLPANVTQVVVPDTLRVASILAHTLYDYPSHQ
                     LVTFGVTGTNGKTSIATMIHLIQRKLQKNSAYLGTNGFQINETKTKGANTTPETVSLT
                     KKIKEAVDAGAESMTLEVSSHGLVLGRLRGVEFDVAIFSNLTQDHLDFHGTMEAYGHA
                     KSLLFSQLGEDLSKEKYVVLNNDDSFSEYLRTVTPCEVFSYGIDEEAQFMAKNIQESL
                     QGVSFDFVTPFGTYSVKSPYVGKFNISNIMAAMIAVWSKGTSLETIIKAVENLEPVEG
                     RLEVLDPSLPIDLIIDYAHTADGMNKLIDAVQPFVKQKLIFLVGMAGERDLTKTPEMG
                     RVACRADYVIFTPDNPANDDPKMLTAELAKGATHQNYIEFDDRAEGIKHAIDIAEPGD
                     TVVLASKGREPYQIMPGHIKVPHRDDLIGLEAAYKKFGGGPVGQ"
     gene            1653..1913
                     /locus_tag="POB55_001759"
     CDS             1653..1913
                     /locus_tag="POB55_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueH family protein"
                     /protein_id="HDI6500721.1"
                     /translation="MALLVNKRFIDEGKTIDVYLFEALNDQIIIAIPDWFWSYQMAMT
                     LDEETCFEAILMQLFVFKEEEEAESIASQLTDWIETYKKEKD"
     gene            1913..3475
                     /locus_tag="POB55_001760"
     CDS             1913..3475
                     /locus_tag="POB55_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HDI6500722.1"
                     /translation="MNLKQEVESRKTFAIISHPDAGKTTLTEKLLYFSGAIREAGTVK
                     GKKTGKFATSDWMKVEQERGISVTSSVMQFDYDDYKINILDTPGHEDFSEDTYRTLMA
                     VDSAVMVIDCAKGIEPQTLKLFKVCKMRGIPIFTFINKLDRVGKEPFELLDEIEETLN
                     IETYPMNWPIGMGQSFFGIIDRKSKTIEPFRDEENILHLNDDFELEEDHAITNDSAFE
                     QAIEELMLVEEAGEAFDNDALLSGDLTPVFFGSALANFGVQNFLNAYVDFAPMPNARQ
                     TKEEVEVSPFDDSFSGFIFKIQANMDPKHRDRIAFMRVVSGAFERGMDVTLQRTNKKQ
                     KITRSTSFMADDKETVNHAVAGDIIGLYDTGNYQIGDTLVGGKQTYSFQDLPQFTPEI
                     FMKVSAKNVMKQKHFHKGIEQLVQEGAIQYYKTLHTNQIILGAVGQLQFEVFEHRMKN
                     EYNVDVVMEPVGRKIARWIENEDQITDKMNTSRSILVKDRYDDLVFLFENEFATRWFE
                     EKFPEIKLYSLL"
     gene            3777..4580
                     /locus_tag="POB55_001761"
     CDS             3777..4580
                     /locus_tag="POB55_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HDI6500723.1"
                     /translation="MLMDPSLILPYLWVLVVLVFLEGLLAADNAIVMAVMVKHLPPEQ
                     RKKALFYGLLGAFVFRFLALFLISIIANFWFIQAAGAVYLIYMSIKNLWQFFKHPEIE
                     SPEAGDNHHYDESGEEIKASNKSFWGTVLKIEFADIAFAIDSMLAALAIAVTLPKVGI
                     HFGGMDLGQFVVMFLGGMIGVILMRYAATWFVELLNKYPGLEGAAFAIVGWVGVKLVV
                     MVLAHPDIAVLPEHFPHGVLWQSIFWTVLIGLVIIGWLGSVVKNKKSHK"
     gene            4799..7123
                     /locus_tag="POB55_001762"
     CDS             4799..7123
                     /locus_tag="POB55_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDI6500724.1"
                     /translation="MEWTLVDIGKKHVIPKSQYRRKRREFFHNEDREENLNQHQDKQN
                     IDNTTSKKADKQIHKDSIDKHERFKNSLSSHLEQRNRDVNENKAEESKSNQDSKSAYN
                     RDHYLTDDVSKKQNSLDSVDQDTEKSKYYEQNSEATLSTKSTDKVESTDMRKLSSDKN
                     KVGHEEQHVLSKPSEHDKETRIDFESSRTDSDSSMQTEKIKKDSSDGNKSSNLKSEVI
                     SDKSNTVPKLSESDDEVNNQKPLTLPEEQKLKRQQSQNEQTKTYTYGDSEQNDKSNYE
                     NDLSHHMPSISDDKDNVMRENHIVDDNPDNDINTPSLSKTDDDRKLDEKIHVEDKHKQ
                     NADSSETVGYQSQSSASHRITEKRNNAINDHDKLNGQKPNAKTSANNNQKKATSKLNK
                     GRATNNNYSDILKKFWMMYWPKLVILMGIIILIVILNAIFNNVNKNDRMNDNNDADAQ
                     KYTTTMKNANNTVKSVVTVENETSKDSSLPKDKASQDEVGSGVVYKKSGDTLYIVTNA
                     HVVGDKENQKITFSNNKSVVGKVLGKDKWSDLAVVKATSSDSSVKEIAIGDSNNLVLG
                     EPILVVGNPLGVDFKGTVTEGIISGLNRNVPIDFDKDNKYDMLMKAFQIDASVNPGNS
                     GGAVVNREGKLIGVVAAKISMPNVENMSFAIPVNEVQKIVKDLETKGKIDYPDVGVKM
                     KNIASLNSFERQAVKLPGKVKNGVVVDQVDNNGLADQSGLKKGDVITELDGKLLEDDL
                     RFRQIIFSHKDDLKSITAKIYRDGKEKEINIKLK"
     gene            7140..8498
                     /locus_tag="POB55_001763"
     CDS             7140..8498
                     /locus_tag="POB55_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HDI6500725.1"
                     /translation="MSIFSQFLKRSSPQQGIVLYYIVAIVIAFLLLNLPYVHKPGVEV
                     NPIDTLFVAVSGISVTGLSPISIVDTYSTFGQLIILVILNIGGIGVMAIGTMLWVVLG
                     KHIGIRERQLIMLDNNKNTMSGTVKLIIDIVKSIFVIELVGAMLLAFYFYRDNPDLKY
                     AIMQGVFVSISATTNGGLDITGKSLIPYAHDYFVQAIVIFLIILGSIGFPVLLEVKAY
                     IQNRVTNFRFSLFTKITTSTYLFLFIVGVLAILLFEHNHAFKGLSWHQSLFYSLFQSA
                     TTRSAGLQTIDVTTLSDPTNIIMGILMFIGSSPSSVGGGIRTTTFAILILFLINFSNN
                     ADKTSIKVYNREVHIMDIQRSFAVFTMATILTFLGMLIISATENGKLTFLQVFFEVMS
                     AFGTCGLSLGVTSDISDISKVVLMILMFIGRVGLISFIIMIAGRREPDKFHYPKERIQ
                     IG"
     gene            8637..>10040
                     /locus_tag="POB55_001764"
     CDS             8637..>10040
                     /locus_tag="POB55_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HDI6500726.1"
                     /translation="MEKNENINVEILTTSDMHSHFLNGDYGSNIYRAGTYVNQVRAQN
                     HRVILLDSGGSLAGSLAAYYYAIVAPYKRHPMIKLMNRMHYDASGVSPSEFKFGLSFL
                     TRSIALARFPWLSANIEYNVTKEPYFSTPYCIKHFGDLKIAIVGVTADGLMENEYSEM
                     EQDVSIEKTLVASKRWIRYIHEVEEPDFLIVIYHGGLNKISNSTKNKKASSNEAEKLM
                     EELGVIDLMITAHQHQTIVGQDHETYYVQAGQDAKELVHLSINFKKRTTTYDVESIDS
                     KVIDLNEYEEDQELLDLTFYDRKAVAYWSQEIISDKGLMLSVNGLQDLVCQTHPFSQL
                     LHDAIHLAFDDDITCVHVPMNGEKGLSGQIRNEDLYHAYPYPDKPMDMTISGQNIKDI
                     LEYSYSHLDFVNEQLSLTIIDETLCTMWQGFNYEIDMNQEPGQRVMLDQIDLSKSYRV
                     TMTDYCYRNYKNYLKNAI"
BASE COUNT         3596 a         1338 c         1961 g         3145 t
ORIGIN      
        1 acaagatggt tttaataatt taagatgagc cataattgtg aaactaattc ataataacgt
       61 atgttttatt tttaatagta gaaattagaa aatgctgatt agtaggatat aacagtgaaa
      121 ttataaattt attaacatca acaaaacgtg tataataaac atattgtaga aaaaggagcg
      181 gttcagtttg gatgcaagta cgttgtttaa gaaagtaaaa gtaaagcgtg tattgggttc
      241 tttagaacaa caaatagatg atatcactac tgattcacgt acagcgagag aaggtagcat
      301 ttttgtcgct tcagttggat atactgtaga cagtcataag ttctgtcaaa gtgtagctga
      361 tcaagggtgt aagttggtag tggtcaataa agaacaatca ttaccagcta acgtaacaca
      421 agtggttgtg ccggacacat taagagtagc tagtattcta gcacacacat tatatgatta
      481 tccgagtcat cagttagtga catttggtgt aacgggtaca aatggtaaaa cttctattgc
      541 gacgatgatt catttaattc aaagaaagtt acaaaaaaat agtgcatatt taggaactaa
      601 tggtttccaa attaatgaaa caaagacaaa aggtgcaaat acgacaccag aaacagtttc
      661 tttaactaag aaaattaaag aagcagttga tgcaggcgct gaatccatga cattagaagt
      721 atcaagccat ggcttagtat taggacgact gcgaggcgtt gaatttgacg ttgcaatatt
      781 ttcaaattta acacaagacc atttagattt tcatggcaca atggaagcat acggacacgc
      841 gaagtcttta ttgtttagtc aattaggtga agatttgtcg aaagaaaagt atgtcgtgtt
      901 aaacaatgac gattcatttt ctgagtattt aagaacagtg acgccttgtg aagtatttag
      961 ttatggaatt gatgaggaag cccaatttat ggctaaaaat attcaagaat ctttacaagg
     1021 tgtcagcttt gattttgtaa cgccttttgg aacttactca gtaaaatcgc cttatgttgg
     1081 taagtttaat atttctaata ttatggcggc aatgattgcg gtgtggagta aaggtacatc
     1141 tttagaaacg attattaaag ctgttgaaaa tttagaacct gttgaagggc gattagaagt
     1201 tttagatcct tcgttaccta ttgatttaat tatcgattat gcacatacag ctgatggtat
     1261 gaacaaatta atcgatgcag tacagccttt tgtaaagcaa aagttgatat ttttagttgg
     1321 tatggcaggc gaacgtgatt taactaaaac gcctgaaatg gggcgagttg cctgtcgtgc
     1381 agattatgtc attttcacac cggataatcc ggcaaatgat gacccgaaaa tgttaacggc
     1441 agaattagcc aaaggtgcaa cacatcaaaa ctatattgaa tttgatgatc gtgcagaagg
     1501 gataaaacat gcaattgaca tagctgagcc tggggatact gtcgttttag catcaaaagg
     1561 aagagaacca tatcaaatca tgccagggca tattaaggtg ccacatcgag atgatttaat
     1621 tggccttgaa gcagcttaca aaaagttcgg tggtggccct gttggtcaat aaaagattta
     1681 ttgatgaagg taaaactatt gatgtttatt tattcgaagc attaaatgac cagataatca
     1741 ttgctatacc agattggttt tggtcatatc agatggcaat gacattagat gaagaaactt
     1801 gttttgaagc aatactcatg caattgtttg tttttaaaga agaggaagag gcagaatcga
     1861 ttgcatcaca actaacagat tggatagaaa catataaaaa ggagaaagac taatgaactt
     1921 aaagcaagaa gttgagtcta gaaagacttt tgcgattatt tcacatcccg atgcagggaa
     1981 aacaacgtta actgaaaaac tattgtactt cagtggtgcc attcgtgaag cgggtacagt
     2041 taaagggaag aagactggta aatttgcgac aagtgactgg atgaaagttg aacaagaacg
     2101 tggtatttct gtaacaagtt cagtaatgca atttgattac gatgattata aaatcaatat
     2161 cttagataca ccaggacatg aagacttttc agaagatacg tatagaacat taatggcagt
     2221 tgacagtgct gtcatggtca tagactgtgc aaaaggtatt gaaccacaaa cattgaagtt
     2281 atttaaagtt tgtaaaatgc gtggtattcc aatctttaca ttcattaata aattagaccg
     2341 agtaggtaaa gaaccatttg aattattaga tgaaatcgaa gagacattaa atattgaaac
     2401 ataccctatg aattggccaa ttggtatggg acaaagtttc tttggcatca ttgatagaaa
     2461 gtctaaaaca attgaaccat ttagagatga agaaaatata ttacatttga atgatgattt
     2521 tgagttggaa gaagatcatg caattacaaa tgatagtgct tttgaacaag cgattgaaga
     2581 attaatgttg gttgaagaag cgggtgaagc ctttgataat gacgcgctgt tgagtggaga
     2641 cttaacacct gtatttttcg gttcagcttt agctaacttt ggtgtacaaa atttcttaaa
     2701 tgcatatgtt gattttgcgc caatgccaaa tgcaagacaa acaaaagaag aggttgaagt
     2761 aagcccgttt gatgattcat tttcaggatt tatctttaaa attcaagcca acatggaccc
     2821 taaacaccgt gatagaattg catttatgcg tgtcgttagt ggtgcatttg aacgtggtat
     2881 ggatgttact ttgcaacgta ctaataaaaa gcaaaagatc acacgttcaa cgtcatttat
     2941 ggcagacgat aaagaaactg tgaatcatgc tgtagcaggc gatatcattg gactatatga
     3001 tactggtaat tatcaaattg gagatacttt agttggtgga aaacaaacct acagtttcca
     3061 agatttacca caatttacgc cagaaatttt tatgaaagtt tctgctaaaa acgtcatgaa
     3121 acagaagcat ttccataaag gtattgaaca attagtacaa gaaggtgcga ttcaatacta
     3181 taaaacatta cacacaaacc aaattatttt aggtgctgtt ggtcagttac aatttgaagt
     3241 tttcgaacat agaatgaaaa atgaatataa tgttgatgtt gttatggagc cagtaggccg
     3301 taaaattgca cgttggattg aaaatgaaga ccaaattaca gataagatga acacatcaag
     3361 atcgatttta gtgaaagata gatatgacga tttagtattc ttatttgaaa atgaatttgc
     3421 aacaagatgg tttgaagaga aattccctga aattaaattg tatagtttac tttaacagct
     3481 caattgtata atcgaatttg ttacattaaa aataattgtt tcgttgaaga aaaataaatt
     3541 gtatatttta aaagaaaaag gtatactatg atgtatcaaa tgaataacct atggcatttt
     3601 gtcagagggg agtaacttaa gaatcatgac cgtataaatg attcgacact ttatcgtcat
     3661 tacgaagata tcttccggta aagtgggcaa tttaaattgc ttagtgagac ctttgctatt
     3721 tatttagcat aggtcttttt gtttgtactt aacttattta tttaaaggag ttgtacatgt
     3781 taatggatcc aagtttgatc ttaccttatt tatgggtact tgtcgtttta gtatttttag
     3841 aaggcttatt agcagcagat aacgcgattg ttatggctgt aatggttaag cacttaccac
     3901 ccgaacaacg taaaaaagct ttgttttacg gtttgttagg tgcatttgta tttagatttt
     3961 tagcattatt cttaattagt attatcgcga acttttggtt tattcaagct gcaggagcgg
     4021 tttacttaat ttatatgtca atcaaaaatc tgtggcagtt ctttaaacac ccagaaattg
     4081 aaagtcctga agctggagat aatcatcatt atgatgaatc tggtgaagag attaaagcaa
     4141 gtaacaaatc attctgggga actgtgttga aaatagaatt tgcagatatc gcatttgcca
     4201 ttgattctat gcttgctgct ttagctattg ctgtaacact tcctaaagtt ggtattcact
     4261 ttggtggtat ggacttaggt cagttcgtag tcatgttcct aggtggaatg attggtgtta
     4321 ttctaatgcg ttatgcagca acatggtttg tagagctatt aaacaaatat ccaggacttg
     4381 aaggtgcagc cttcgcgatc gttggttggg taggtgttaa attagttgtc atggtattag
     4441 cgcacccaga catcgctgta ttgcctgagc acttcccaca tggcgtatta tggcaatcta
     4501 ttttctggac agtactaatt ggattagtaa ttatcggttg gttaggttca gttgttaaaa
     4561 ataaaaaatc gcataaataa ttgatgtgaa gcggacaatc ttaatttagt ttaaggttgt
     4621 cctttttcat ttaattgagt gatttatgaa aaatggattt tgaagaatgt gaatcaaaag
     4681 atgcgatata gtattaagaa aatgtgcctt ttatatttag cattttttca atagaaatta
     4741 tatagatttt aaagcaaatt aggtgttaat gtgtcataat gataagtgat tttattgaat
     4801 ggagtggaca ttagtggata ttggtaaaaa acatgtaatt cctaaaagtc agtaccgacg
     4861 taagcgtcgt gaattcttcc acaacgaaga cagagaagaa aatttaaatc aacatcaaga
     4921 taaacaaaat atagataata caacatcaaa aaaagcagat aagcaaatac ataaagattc
     4981 aattgataag cacgaacgtt ttaaaaatag tttatcatcg catttagaac agagaaaccg
     5041 tgatgttaat gagaataaag ctgaagaaag taaaagtaat caggatagta agtcagcata
     5101 taacagagat cattatttaa cagacgatgt atctaaaaaa caaaattcat tagattcagt
     5161 ggaccaagat acagagaaat caaaatatta tgagcaaaat tctgaagcga ctttatcaac
     5221 taaatcaacc gataaagtag aatcaactga catgagaaag ctaagttcag ataaaaacaa
     5281 agttggtcat gaagagcaac atgtactttc taaaccttca gaacatgata aagagactag
     5341 aattgatttt gagtcttcaa gaactgattc agacagctcg atgcagacag agaaaataaa
     5401 aaaagacagt tcagatggaa ataaaagtag taatctgaaa tctgaagtaa tatcagacaa
     5461 atcaaataca gtaccaaaat tgtcggaatc tgatgatgaa gtaaataatc agaagccatt
     5521 aactttgccg gaagaacaga aattgaaaag acagcaaagt caaaatgagc aaacaaaaac
     5581 ctatacatat ggtgatagcg aacaaaatga caagtctaat tatgaaaatg atttaagtca
     5641 tcatatgcca tcgataagtg atgataaaga taacgtcatg agagaaaatc atattgttga
     5701 cgataatcct gataatgata tcaatacacc atcattatca aaaacagatg acgatcgaaa
     5761 acttgatgaa aaaattcatg ttgaagataa acataaacaa aatgcagact cgtctgaaac
     5821 ggtgggatat caaagtcagt caagtgcatc tcatcgtatc actgaaaaaa gaaataatgc
     5881 tattaatgac catgataaat taaacggtca aaaaccaaat gcaaagacat cggcaaataa
     5941 taatcaaaaa aaggctacat caaaattgaa caaagggcgc gctacaaata ataattatag
     6001 tgacattttg aaaaagtttt ggatgatgta ttggcctaaa ttagttattc taatgggtat
     6061 tattattcta attgttattt tgaatgctat ttttaataat gtgaacaaaa atgatcgcat
     6121 gaatgataat aatgatgcag atgctcaaaa atatacgaca acgatgaaaa atgccaataa
     6181 cacagttaaa tcggtcgtta cagttgaaaa tgaaacatca aaagattcat cattacctaa
     6241 agataaagca tctcaagacg aagtaggatc aggtgttgta tataaaaaat ctggagatac
     6301 gttatatatt gttacgaatg cacacgttgt cggtgataaa gaaaatcaaa aaataacttt
     6361 ttcgaataat aaaagtgttg ttgggaaagt gcttggtaaa gataaatggt cagatttagc
     6421 tgttgttaaa gcgacttctt cagacagttc agtgaaagag atagctattg gagattcaaa
     6481 taatttagtg ttaggagagc caatattagt cgtaggtaat ccacttggtg tagactttaa
     6541 aggcactgtg acagaaggta ttatttcagg tctgaacaga aatgttccta ttgatttcga
     6601 taaagataat aaatatgata tgttgatgaa agctttccaa attgatgcat cagtaaatcc
     6661 aggtaactcg ggtggtgctg tagtcaatag agaaggaaaa ttaattggtg tagttgcagc
     6721 taaaattagt atgccaaacg ttgaaaatat gtcatttgca atacctgtta atgaagtaca
     6781 aaagattgta aaagatttag aaacaaaagg taaaattgac tatcccgatg taggtgttaa
     6841 aatgaagaat attgccagtc taaatagttt tgaaagacaa gcagttaaat tgccaggaaa
     6901 agttaagaac ggtgttgttg tagatcaagt tgacaacaat ggtttagcag atcaatctgg
     6961 tctgaaaaaa ggtgatgtaa ttactgaatt agatggcaaa cttttagaag atgatttacg
     7021 ctttaggcag attatattta gtcataaaga tgacttgaaa tcaattacag cgaagattta
     7081 tagagatggt aaagagaaag aaattaatat taaactaaaa taatgttgag gtgaacaacg
     7141 tgtcaatttt tagccagttt ttaaaaagat caagccctca acaaggtatt gtattgtact
     7201 atatcgtcgc aattgtcatt gcatttttat tattaaactt accgtatgtt cataaaccag
     7261 gtgtagaagt aaatccaatt gacacattat ttgttgccgt atccggaatt agtgttactg
     7321 gattgtctcc gataagtatt gtcgatacct attctacatt tggacaatta attatcctcg
     7381 tgatattaaa tattggtgga attggcgtca tggcaattgg tacgatgtta tgggtggtac
     7441 taggtaaaca tattggaatt agagaacgtc agttaattat gttagataat aacaaaaaca
     7501 caatgagtgg taccgtcaaa ttgattattg atattgtaaa atcaatattt gtaatcgaac
     7561 tcgtaggagc catgttatta gcattttact tttatcgaga taatccagat ttaaaatatg
     7621 caatcatgca aggtgttttt gtttctattt ctgccactac caatggtgga ttagatatta
     7681 caggtaagtc attaattcct tatgcacatg attattttgt acaagcgata gttatatttt
     7741 taataatttt aggatcaatc ggcttcccag tattattaga agttaaagct tatattcaaa
     7801 atagggttac taattttaga ttttcattat ttactaaaat tacgacatca acatatttat
     7861 tcctatttat tgttggggta ttagccattc tattatttga acataaccat gcgttcaaag
     7921 gattaagttg gcatcaatcg ttattctatt cgctgtttca atcagcgact acaagaagtg
     7981 cgggtcttca aacaattgat gtgacaacac taagtgaccc cactaatatt atcatgggta
     8041 ttttaatgtt tataggatct tcgccaagtt cggttggtgg cggtattcgt acaacaactt
     8101 tcgctatttt aattttgttt ttaattaact ttagtaataa tgccgataaa acatccatta
     8161 aagtttacaa tagagaagta cacattatgg atattcaacg ttcatttgca gtatttacaa
     8221 tggcgacaat tttaacattt ttaggaatgc taattatatc agctactgaa aatggtaagc
     8281 ttacattttt acaagtattt tttgaagtca tgtctgcatt tggaacttgt ggactatcgc
     8341 ttggtgtcac aagtgatatt agtgatattt ctaaggtcgt actaatgata ctcatgttta
     8401 taggacgtgt tggcttaata tcatttatca ttatgatagc aggacgtcga gaaccagata
     8461 aattccatta tccaaaagaa cgtattcaaa taggataata taatagcaat ctaagtttag
     8521 ttaatgtaga ttttaactgg aacttagatt gcttttttag tttgtatttt taacttattt
     8581 tataagacga ttggtttcga aaatggtaaa ctagtaacaa tgagaggtgt aacatgatgg
     8641 aaaaaaatga aaacattaat gtagagattt taactacgtc agatatgcat agtcatttct
     8701 taaatggtga ttatggttca aatatttata gagctggtac ttatgttaac caagtaagag
     8761 cacaaaatca tcgcgtcatt ttattagata gtggcggaag tttagctggc tcgttagcgg
     8821 cctattatta tgctattgtt gcaccttata aacgacatcc aatgataaag ttaatgaaca
     8881 gaatgcatta tgatgctagc ggtgtgagtc caagtgaatt caagtttggt ttatcatttt
     8941 taactcgttc aattgctttg gcacgttttc catggttatc agcaaatatt gaatacaatg
     9001 ttactaagga gccttatttt tcaactccat attgtattaa acattttggt gacttaaaaa
     9061 ttgctatcgt aggcgttaca gcagatggtt taatggaaaa tgagtattct gaaatggagc
     9121 aagatgtatc tattgaaaag acattagtgg catcaaaacg atggattaga tatatccatg
     9181 aagttgaaga gccggatttt ttgattgtaa tttatcatgg tggattgaat aaaattagta
     9241 atagtacgaa aaataaaaag gcaagttcga atgaagctga aaaattaatg gaagaactcg
     9301 gtgttataga tttaatgatt acagctcatc agcatcaaac aatagtcggt caagatcatg
     9361 aaacgtatta tgttcaggct ggtcaagatg caaaagagct tgtacatctt tcgattaatt
     9421 ttaaaaagag aacaacaact tatgatgttg aaagcattga ttctaaagtg attgacttaa
     9481 atgagtatga agaggaccaa gaattattag atttaacatt ctatgataga aaagcagtgg
     9541 cttattggtc acaggaaatc ataagtgata aaggtttgat gttatcagta aatgggttac
     9601 aagatttagt ctgtcaaaca catccatttt cacaattatt acatgatgca attcaccttg
     9661 catttgatga tgatataaca tgtgtccacg tgcctatgaa cggagagaag gggttgagtg
     9721 gacagattcg aaatgaagat ttgtatcatg catacccata tccagataag ccaatggata
     9781 tgacaattag tggtcaaaat atcaaagata tattggagta tagttattca catttagatt
     9841 ttgttaacga gcaattaagc ttaacaatta ttgatgaaac gttatgtaca atgtggcaag
     9901 gattcaatta tgagattgat atgaatcaag aacctgggca acgagtaatg ttagatcaaa
     9961 ttgatttgag taagagttat agagttacaa tgactgacta ttgttatcgt aactacaaga
    10021 attatttaaa aaatgctatt
//
LOCUS       DAMZKC010000097         9811 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.098, whole genome shotgun sequence.
ACCESSION   DAMZKC010000097 DAMZKC010000000
VERSION     DAMZKC010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9811
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.098"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            113..1408
                     /gene="purB"
                     /locus_tag="POB55_001765"
     CDS             113..1408
                     /gene="purB"
                     /locus_tag="POB55_001765"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HDI6500727.1"
                     /translation="MIERYSREEMSNIWTDQNRYEAWLEVEILACEAWSELGHIPKAD
                     VQKIRQNAKVNVERAQEIEQETRHDVVAFTRQVSETLGEERKWVHYGLTSTDVVDTAL
                     SFVIKQANDIIEKDLERFIDVLAEKAKNYKYTLMMGRTHGVHAEPTTFGVKMALWYTE
                     MQRNLQRFKQVREEIEVGKMSGAVGTFANIPPEIESYVCKHLGIGTAPVSTQTLQRDR
                     HAYYIATLALIATSLEKFAVEIRNLQKTETREVEEAFAKGQKGSSAMPHKRNPIGSEN
                     ITGISRVIRGYITTAYENVPLWHERDISHSSAERIMLPDVTIALDYALNRFTNIVDRL
                     TVFEDNMRNNIDKTFGLIFSQRVLLALINKGMVREEAYDKVQPKAMISWETKTPFREL
                     IEQDESITSVLTKEELDECFDPKHHLNQVDTIFERAGLA"
     gene            1517..1819
                     /locus_tag="POB55_001766"
     CDS             1517..1819
                     /locus_tag="POB55_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500728.1"
                     /translation="MQIEKLRGAALDELFDAILTLENREECYQFFDDLCTVNEIQSLS
                     QRLQVAKMIKQGYTYATIEQESGASTATISRVKRSLQWGNDAYTMILDRMNIETNE"
     gene            1991..2683
                     /locus_tag="POB55_001767"
     CDS             1991..2683
                     /locus_tag="POB55_001767"
                     /EC_number="2.5.1.n9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heptaprenylglyceryl phosphate synthase"
                     /protein_id="HDI6500729.1"
                     /translation="MYDIKKWRHIFKLDPAKHISDDDLDAICMSQTDAIMIGGTDDVT
                     EDNVIHLMSRVRRYPLPLVLEISNIESVMPGFDFYFVPTVLNSTDVVFHNGTLLEALK
                     TYGHSIDFEEVIFEGYVVCNADSKVAKHTKANTDLTTEDLEAYAQMVNHMYRLPVMYI
                     EYSGIYGDVSKVQAVSEHLTETQLFYGGGISSEQQATEMAAIADTIIVGDIIYKDIKK
                     ALKTVKIKESSK"
     gene            2680..4872
                     /gene="pcrA"
                     /locus_tag="POB55_001768"
     CDS             2680..4872
                     /gene="pcrA"
                     /locus_tag="POB55_001768"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HDI6500730.1"
                     /translation="MNALLNHMNTEQSEAVKTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     LDEKDVSPYNVLAITFTNKAAREMKERVQKLVGDQAEVIWMSTFHSMCVRILRRDADR
                     IGIERNFTIIDPTDQKSVIKDVLKNENIDSKKFEPRMFIGAISNLKNELKTPADAQKE
                     ATDYHSQMVATVYSGYQRQLSRNEALDFDDLIMTTINLFERVPEVLEYYQNKFQYIHV
                     DEYQDTNKAQYTLVKLLASKFKNLCVVGDSDQSIYGWRGADIQNILSFEKDYPEANTI
                     FLEQNYRSTKTILNAANEVIKNNSERKPKGLWTANTNGEKIHYYEAMTERDEAEFVIR
                     EIMKHQRNGKKYQDMAILYRTNAQSRVLEETFMKSNMPYTMVGGQKFYDRKEIKDLLS
                     YLRIIANSNDDISLQRIINVPKRGVGPSSVEKVQNYALQNNISMFDALGEADFIGLSK
                     KVTQECLNFYELIQSLIKEQEFLEIHEIVDEVLQKSGYREMLERENTLESRSRLENID
                     EFMSVPKDYEENTPLEEQSLINFLTDLSLVADIDEADTENGVTLMTMHSAKGLEFPIV
                     FIMGMEESLFPHIRAIKSEDDHEMQEERRICYVAITRAEEVLYITHATSRMLFGRPQS
                     NMPSRFLKEIPESLLENHSSGKRQTIQPKAKPFAKRGFSQRTTSTKKQVLSSDWNVGD
                     KVMHKAWGEGMVSNVNEKNGSIELDIIFKSQGPKRLLAQFAPIEKKED"
     gene            4876..6879
                     /gene="ligA"
                     /locus_tag="POB55_001769"
     CDS             4876..6879
                     /gene="ligA"
                     /locus_tag="POB55_001769"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HDI6500731.1"
                     /translation="MADLSSRVNELHDLLNQYSYEYYVEDNPSVPDSEYDKLLHELIK
                     IEEEHPEYKTVDSPTVRVGGEAQASFNKVNHDTPMLSLGNAFNEDDLRKFDQRIREQI
                     GNVEYMCELKIDGLAVSLKYVDGYFVQGLTRGDGTTGEDITENLKTIHAIPLKMKEPL
                     NVEVRGEAYMPRRSFLRLNEEKEKNDEQLFANPRNAAAGSLRQLDSKLTAKRKLSVFI
                     YSVNDFTDFNARSQSEALDELDKLGFTTNKNRARVNNIDGVLEYIEKWTSQRESLPYD
                     IDGIVIKVNDLDQQDEMGFTQKSPRWAIAYKFPAEEVVTKLLDIELSIGRTGVVTPTA
                     ILEPVKVAGTTVSRASLHNEDLIHDRDIRIGDSVVVKKAGDIIPEVVRSIPERRPEDA
                     VTYHMPTHCPSCGHELVRIEGEVALRCINPKCQAQLVEGLIHFVSRQAMNIDGLGTKI
                     IQQLYQSELIKDVADIFYLTEEDLLPLDRMGQKKVDNLLAAIQQAKDNSLENLLFGLG
                     IRHLGVKASQVLAEKYETIDRLLTVTEAELVEIHDIGDKVAQSVVTYLENEDIRALIQ
                     KLKDKHVNMIYKGIKTSDIEGHPEFSGKTIVLTGKLHQMTRNEASKWLASQGAKVTSS
                     VTKNTDVVIAGEDAGSKLTKAQSLGIEIWTEQQFVDKQNELNS"
     gene            6892..8091
                     /locus_tag="POB55_001770"
     CDS             6892..8091
                     /locus_tag="POB55_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CamS family sex pheromone protein"
                     /protein_id="HDI6500732.1"
                     /translation="MKRTLVLLITAIFILAACGNHKDDQAGKDNQKHNNSSNQVKEIA
                     TDKNVQGDNYRTLLPFKESQARGLLQDNMANSYNGGDFEDGLLNLSKEVFPTDKYLYQ
                     DGQFLDKKTINAYLNPKYTKREIDKMSEKDKKDKKANENLGLNPSHEGETDPEKIAEK
                     SPAYLSNILEQDFYGGGDTKGKNIKGMTIGLAMNSVYYYKKEKDGPTFSKKLDDSEVK
                     KQGKQMASEILSRLRENDDLKDIPIHFAIYKQSSEDSITPGEFITQATAEKSQTKLNE
                     WHNINEKSALLPSSTAVDYDENLNNNFKQFNDNLQSYFSNFTQAVGKVKFVDKKPQRL
                     VVDLPIDYYGQAETIGITQYVTEQANKYFDKIDNYEIRIKDGNQPRALISKTKDDKEP
                     QVHIYSN"
     gene            complement(8180..9718)
                     /gene="putP"
                     /locus_tag="POB55_001771"
     CDS             complement(8180..9718)
                     /gene="putP"
                     /locus_tag="POB55_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HDI6500733.1"
                     /translation="MLTMGTALSQQVDANWQTYIMIAVYFLILIVIGFYGYKQATGNL
                     SEYMLGGRSIGPYITALSAGASDMSGWMIMGLPGSVYSTGLSAMWITIGLTLGAYINY
                     FVVAPRLRVYTELAGDAITLPDFFKNRLNDKNNVLKIISGLIIVVFFTLYTHSGFVSG
                     GKLFESAFGLDYHFGLILVAFIVIFYTFFGGYLAVSITDFFQGVIMLIAMVMVPIVAM
                     MNLNGWGTFHDVAAMKPTNLNLFKGLSFIGIISLFSWGLGYFGQPHIIVRFMSIKSHK
                     MLPKARRLGISWMAVGLLGAVAVGLTGIAFVPAYHIKLEDPETLFIVMSQVLFHPLVG
                     GFLLAAILAAIMSTISSQLLVTSSSLTEDFYKLIRGEEKAKTHQKEFVMIGRLSVLVV
                     AIVAIAIAWNPNDTILNLVGNAWAGFGASFSPLVLFALYWKGLTRAGAVSGMVSGALV
                     VIVWIAWIKPLAHINEIFGLYEIIPGFIVSVIVTYVVSKLTKKPGAFVETDLNKVRDI
                     VREK"
BASE COUNT         3686 a         1456 c         1832 g         2837 t
ORIGIN      
        1 ccaattctgt taaactgtga agagtagagt taaaaacgaa ctttttgttt atttttatta
       61 aaaatgttct gtttttgaaa attcgagtat acatacgata ggagttaaaa aaatgattga
      121 acgctattct agagaagaaa tgtctaatat ttggactgat caaaatcgct atgaagcatg
      181 gttagaagtg gaaattttag catgtgaagc atggagtgaa ttaggacata taccgaaagc
      241 tgacgtacaa aaaattcgtc aaaacgcaaa ggtaaatgtc gaacgtgcac aagaaattga
      301 acaagaaacg cgccatgatg ttgtagcctt tactagacaa gtttctgaaa cgctaggtga
      361 agaacgtaag tgggtacatt atggtttaac ttctactgat gttgtagata cagctttaag
      421 tttcgttatt aaacaagcaa atgacattat tgaaaaagat ttagaaagat ttatcgacgt
      481 tttagctgaa aaagcaaaaa attataaata tacattgatg atgggacgta cacatggagt
      541 gcatgcagaa ccaactacat ttggtgtcaa aatggcattg tggtacacgg aaatgcaacg
      601 taacttacaa cgtttcaaac aagtaagaga agaaattgaa gtagggaaaa tgagtggtgc
      661 agtaggtact tttgctaaca ttcctcctga aatagaaagt tatgtgtgta aacatttagg
      721 tattggcaca gcgcctgtat caacgcaaac attgcaacgc gatcgacatg catactatat
      781 tgcgacatta gcattaatcg cgacgtcact agaaaaattt gccgttgaaa ttcgtaacct
      841 tcaaaaaacc gaaacaagag aagttgaaga agcatttgca aaaggccaaa aaggttcatc
      901 tgcaatgcct cataaacgaa atccaattgg ttctgaaaat atcacaggta tttcaagagt
      961 cattcgtggt tatattacta cagcttatga gaatgtgcca ttatggcatg aaagagatat
     1021 ttctcattct tctgctgagc gtattatgtt accagatgtt acaatagcac tagattatgc
     1081 attgaatcgt ttcacaaata ttgtggaccg tttaactgta tttgaagata atatgcgtaa
     1141 taacatagat aaaacatttg gtctgatttt ctcacaacgt gtcttattag cattaattaa
     1201 taaaggtatg gttcgtgaag aagcatacga caaagtacaa ccaaaagcga tgatatcttg
     1261 ggaaacgaaa acacctttcc gtgaattaat cgaacaagat gagtctatta caagtgtttt
     1321 aacaaaagaa gaattggatg aatgtttcga tcctaaacat catttgaatc aagttgacac
     1381 tattttcgaa cgcgctggat tagcataaaa ttaaataaag taacgattca gatttaataa
     1441 aatataaatc gctacacttt aatagattaa tgcgttaaaa tgacataaag aataaaatta
     1501 taggggttgt ttcgatatgc aaattgaaaa attacgaggt gcagcgttag atgaattgtt
     1561 tgatgcaata ctaacgttag aaaatagaga agaatgttac caatttttcg atgatttgtg
     1621 tactgtaaat gaaattcaat cactgtctca aagattacaa gttgctaaaa tgattaagca
     1681 aggttatacc tatgcaacga ttgaacaaga atctggagca tcgactgcaa cgatttctag
     1741 agtgaagcgt tcattacaat ggggtaatga tgcttataca atgattttag atcgtatgaa
     1801 tattgaaaca aatgaataat aaatgaacga taaacaatgg ttatctatct gcactaataa
     1861 ggtagataat cattgttttt tcacgaaaaa atttacagag taaaagaact taaatttcat
     1921 attaagtctt tagaactcga cacttaaaaa tgctataatc atatgtatgt taaaaaagga
     1981 gtttcggaaa atgtatgaca ttaaaaaatg gcgccatatt tttaaattag acccagctaa
     2041 acatatttca gatgatgatt tagatgcgat ttgtatgtct caaacagatg caattatgat
     2101 tggtggaact gatgacgtta ctgaagataa tgtcattcat ttaatgagca gagtaagaag
     2161 atacccattg cctttagtac ttgaaatatc aaacatcgaa agtgtaatgc ctggttttga
     2221 tttttatttt gtacctacag tactgaacag tacagatgtt gtatttcaca atggtacatt
     2281 attagaagcg cttaaaacat atggacatag tatagatttt gaggaagtaa tatttgaagg
     2341 gtatgtcgtg tgcaatgctg atagcaaagt ggcaaaacat accaaagcaa atacagattt
     2401 aacaacagaa gatttagaag catatgccca aatggtcaat catatgtatc gattaccggt
     2461 tatgtatata gagtatagtg gcatttatgg cgacgtatca aaggttcaag ctgtctcaga
     2521 acatctaaca gaaacgcaac ttttttatgg tggcggtatt tcctcagaac aacaagcgac
     2581 agagatggca gctattgcag atacaattat cgtcggtgat attatttata aagatattaa
     2641 aaaagcttta aaaacagtaa aaataaagga gtctagtaaa tgaatgcgtt attaaatcat
     2701 atgaatacag agcaaagtga agctgtaaag acaacagaag gaccattgtt aattatggca
     2761 ggtgctggtt cagggaagac acgtgtttta acacatagaa ttgcttattt attagacgaa
     2821 aaagatgtct caccatacaa tgttttggct attactttta caaataaagc tgcaagagaa
     2881 atgaaagaac gtgttcaaaa attagtaggt gatcaagcag aagttatttg gatgtcaaca
     2941 ttccactcaa tgtgtgttcg tattttacgt cgtgatgcag atcgaattgg tatagaacgc
     3001 aattttacga taattgatcc tacagaccaa aaatctgtta ttaaagacgt cttaaaaaat
     3061 gaaaatattg atagtaaaaa gtttgaacct cgtatgttta tcggtgcgat cagtaatttg
     3121 aaaaatgaac ttaaaacacc tgcagatgct caaaaagaag ccacagatta tcactcgcaa
     3181 atggtagcaa cggtttatag tggatatcaa cgccaattgt cacgtaatga agcgttagat
     3241 tttgatgacc ttattatgac aacgattaac ttatttgagc gtgtaccaga agttctagaa
     3301 tattatcaga acaaattcca atatattcat gtagatgagt atcaagatac taataaagca
     3361 caatacacat tagttaaatt attagcaagt aagtttaaaa acttatgtgt tgtaggtgac
     3421 tcagatcagt caatttatgg ttggcgtggt gctgatattc aaaatatctt atcatttgaa
     3481 aaagactatc cagaagcgaa tacaatcttt ttagagcaaa attatcgttc gacgaaaacg
     3541 attttaaatg cggctaacga agtgattaaa aataattctg aacgtaagcc aaaaggactg
     3601 tggactgcaa atacgaatgg tgagaaaatt cattactatg aagcaatgac ggaacgtgat
     3661 gaagcggaat ttgtaatacg agaaattatg aagcatcaac gtaatggtaa gaaatatcaa
     3721 gatatggcaa ttttatatag aacgaatgca caatcacgtg tacttgagga aacattcatg
     3781 aaatctaata tgccatacac aatggttggt ggccaaaagt tctatgaccg taaagaaatc
     3841 aaagatttat taagttattt gcgtataatt gccaatagta atgatgacat tagtttgcaa
     3901 cgtattatta atgttccaaa aagaggtgta ggtccttcat ctgttgaaaa agttcaaaac
     3961 tatgcacttc aaaacaatat cagtatgttt gatgcacttg gagaagctga ttttatcgga
     4021 ttgtcaaaaa aggtgacaca agagtgtctt aatttttacg agttaatcca aagcttgata
     4081 aaagaacaag aatttttaga aattcatgaa atagttgatg aagtattaca aaagtctggc
     4141 tatcgagaaa tgcttgaacg tgaaaataca ttagaatctc gaagtagatt agaaaacatc
     4201 gatgaattta tgtcagtacc aaaagactat gaggaaaata ccccattaga agaacagtca
     4261 ttaattaact ttttaacgga tttatcgtta gtagctgata ttgatgaggc agatactgaa
     4321 aatggcgtaa cactaatgac gatgcactcg gctaagggcc ttgaatttcc aattgtcttt
     4381 ataatgggga tggaagaatc tttattccca catattagag cgattaagag tgaagatgat
     4441 catgaaatgc aagaagaacg tcgtatttgt tatgtagcaa ttacaagggc tgaagaggtg
     4501 ttatatatca ctcatgcgac atcaagaatg ttatttggtc gccctcagtc aaatatgcca
     4561 tccagatttt taaaggaaat tccagaatca ctattagaaa atcattcaag tggcaaacga
     4621 caaacgatac aacctaaggc aaaacctttt gctaaacgcg gatttagtca acgaacaacg
     4681 tcaacgaaaa aacaagtatt gtcatctgat tggaatgtag gtgacaaagt gatgcataaa
     4741 gcctggggag aaggcatggt gagtaatgta aacgagaaaa atggctcaat cgaactagat
     4801 attatcttta aatcacaagg gccaaaacgt ttgttagcgc aatttgcacc aattgaaaaa
     4861 aaggaggatt aagggatggc tgatttatcg tctcgtgtga acgagttaca tgatttatta
     4921 aatcaataca gttatgaata ctatgtagag gataatccat ctgtaccaga tagtgaatat
     4981 gacaaattac ttcatgaact gattaaaata gaagaggagc atcctgagta taagactgta
     5041 gattctccaa cagttagagt tggcggtgaa gcccaagcct ctttcaataa agtcaaccat
     5101 gacacgccaa tgttaagttt agggaatgca tttaatgagg atgatttgag aaaattcgac
     5161 caacgcatac gtgaacaaat tggcaacgtt gaatatatgt gcgaattaaa aattgatggc
     5221 ttagcagtat cattgaaata tgttgatgga tacttcgttc aaggtttaac acgtggtgat
     5281 ggaacaacag gtgaagatat taccgaaaat ttaaaaacaa ttcatgcgat acctttgaaa
     5341 atgaaagaac cattaaatgt agaagttcgt ggtgaagcat atatgccgag acgttcattt
     5401 ttacgattaa atgaagaaaa agaaaaaaat gatgagcagt tatttgcaaa tccaagaaac
     5461 gctgctgcgg gatcattaag acagttagat tctaaattaa cggcaaaacg aaagctaagc
     5521 gtatttatat atagtgtcaa tgatttcact gatttcaatg cgcgttcgca aagtgaagca
     5581 ttagatgagt tagataaatt aggttttaca acgaataaaa atagagcgcg tgtaaataat
     5641 atcgatggtg ttttagagta tattgaaaaa tggacaagcc aaagagagtc attaccttat
     5701 gatattgatg ggattgttat taaggttaat gatttagatc aacaggatga gatgggattc
     5761 acacaaaaat ctcctagatg ggccattgct tataaatttc cagctgagga agtagtaact
     5821 aaattattag atattgaatt aagtattgga cgaacaggtg tagtcacacc tactgctatt
     5881 ttagaaccag taaaagtagc tggtacaact gtatcaagag catctttgca caatgaggat
     5941 ttaattcatg acagagatat tcgaattggt gatagtgttg tagtgaaaaa agcaggtgac
     6001 atcatacctg aagttgtacg tagtattcca gaacgtagac ctgaggatgc tgtcacatat
     6061 catatgccaa cccattgtcc aagttgtgga catgaattag tacgtattga aggcgaagta
     6121 gcacttcgtt gcattaatcc aaaatgccaa gcacaacttg ttgaaggatt gattcacttt
     6181 gtatcaagac aagccatgaa tattgatggt ttaggcacta aaattattca acagctttat
     6241 caaagcgaat taattaaaga tgttgctgat attttctatt taacagaaga agatttatta
     6301 cctttagaca gaatggggca gaaaaaagtt gataatttat tagctgccat tcaacaagct
     6361 aaggacaact ctttagaaaa tttattattt ggtctaggta ttaggcattt aggtgttaaa
     6421 gcgagccaag tgttagcaga aaaatatgaa acgatagatc gattactaac ggtaactgaa
     6481 gcggaattag tagaaattca tgatataggt gataaagtag cacaatctgt agttacttat
     6541 ttagaaaatg aagatattcg tgctttaatt caaaaattaa aagataaaca tgttaatatg
     6601 atttataaag gtatcaaaac atcagatatt gaaggacatc ctgaatttag tggtaaaacg
     6661 atagtactga ctggtaagct acatcaaatg acacgcaatg aagcatctaa atggcttgca
     6721 tcacaaggtg ctaaagttac aagtagcgtt actaaaaata cagatgtcgt tattgctggt
     6781 gaagatgcag gttcaaaatt aacaaaagca caaagtttag gtattgaaat ttggacagag
     6841 caacaatttg tagataagca aaatgaatta aatagttaga ggggtatgtc gatgaagcgt
     6901 acattagtat tattgattac agctatcttt atactcgctg cttgtggtaa ccataaggat
     6961 gaccaggctg gaaaagataa tcaaaaacat aacaatagtt caaatcaagt aaaagaaatt
     7021 gctacggata aaaatgtaca aggtgataac tatcgtacat tgttaccatt taaagaaagc
     7081 caggcaagag gacttttaca agataacatg gcaaatagtt ataatggcgg cgactttgaa
     7141 gatggtttat tgaacttaag taaagaagta tttccaacag ataaatattt gtatcaagat
     7201 ggtcaatttt tggacaagaa aacaattaat gcctatttaa atcctaagta tacaaaacgt
     7261 gaaatcgata agatgtctga aaaagataaa aaagacaaga aagcgaatga aaatttagga
     7321 cttaatccat cacacgaagg tgaaacagat cctgaaaaga ttgcagaaaa atcaccagcc
     7381 tatttatcta acattttaga gcaagatttt tatggtggtg gagatacaaa aggtaagaat
     7441 attaaaggta tgacgattgg tttagctatg aatagtgttt attactataa aaaagaaaaa
     7501 gatggaccga cttttagtaa aaaactagat gatagcgaag ttaaaaagca aggtaaacaa
     7561 atggctagtg agatattatc aaggttacgt gaaaatgatg atttaaaaga tataccaatt
     7621 cattttgcaa tttataagca atcaagtgaa gattcaatca caccaggtga atttatcact
     7681 caagcgactg cagaaaagag tcaaacaaag cttaatgaat ggcataatat caatgaaaaa
     7741 tcagctttat taccttcttc aacagcagta gattatgatg aaaatttaaa taataatttc
     7801 aagcaattta atgataattt gcaatcatat ttttctaatt tcacacaagc agtagggaaa
     7861 gttaaatttg ttgataaaaa gccacaacga ttagtagtag atttaccaat cgattactat
     7921 ggacaagctg aaacaattgg tattacacag tacgttactg aacaagcgaa taaatatttc
     7981 gataaaatcg ataactatga aattcgtatt aaagatggta accaaccacg tgctttaatt
     8041 agtaagacaa aagatgacaa agaaccgcaa gttcatattt acagtaatta attgtaattt
     8101 aaagataata aaaaggtacc aatgttagtc agaattttga ttaacattag taccttttta
     8161 atttgttaag acttatgaat tatttttctc taacgatgtc gcggactttg tttaagtcag
     8221 tttcaacaaa tgcaccaggt tttttagtaa gtttacttac aacatatgta acgattacac
     8281 ttacaataaa tccaggaata atttcatata agccgaatat ttcgttgata tgtgccaatg
     8341 gtttaatcca cgcaatccaa acgataacga ctaaggcacc tgaaaccatt ccacttacag
     8401 caccggcacg tgtcaaacct ttccagtaaa gtgcaaatag cacaagtgga ctgaacgatg
     8461 caccaaatcc ggcccaagcg ttacctacta agtttagaat tgtgtcgttt ggattccatg
     8521 caatcgcgat ggcaacaatt gctacaacta atacagataa tcttccaatc ataacaaatt
     8581 ctttttggtg cgttttagct ttttcttcac cacgaattaa tttataaaag tcttccgtta
     8641 gtgaactaga tgttacaagt aattgtgaag aaatcgtgct cataattgca gctagaatcg
     8701 cagcaagtaa gaaaccacct acaagaggat ggaagagtac ttgactcatc acgatgaata
     8761 atgtctcagg atcttctagt ttaatatgat aagcaggtac gaatgcaata cctgttaaac
     8821 caacagccac agcgcctaat aaaccaacag ccatccagct aatacctaaa cgtctagctt
     8881 taggtagcat cttgtgtgat ttaatagaca taaaccttac aatgatatga ggttgaccga
     8941 aataacctaa tccccatgaa aatagagaga taattcctat aaatgataac cctttaaata
     9001 aatttaaatt tgtaggtttc atagctgcta catcatgaaa cgttccccag ccgtttaaat
     9061 tcatcatagc aacaataggg accataacca tcgcaattaa cataatgaca ccttggaaga
     9121 aatctgtaat tgatacagct aaatatccac caaagaaagt atagaaaatg acaatgaaag
     9181 caactaatat taaaccgaaa tgataatcta atccaaaagc actttcaaat agtttaccac
     9241 cagatacgaa accagaatgt gtatataatg taaagaatac tacgataatc aatccagaaa
     9301 taatctttaa cacattattt ttatcgttta aacgattttt aaagaaatct ggtaatgtaa
     9361 ttgcatctcc agctaattcg gtataaacac gaagtctagg agcaacaaca aagtaattta
     9421 tataagcacc taatgttaaa ccgattgtaa tccacatagc tgatagacca gtgctataga
     9481 cagaaccagg tagccccata atcatccatc cactcatatc tgaagctcca gctgataatg
     9541 cagtaatata cggtccaata ctacgtccac ctaacatgta ctcgcttagg ttaccagttg
     9601 cttgcttgta accgtaaaag ccaataacga ttagtatcaa gaagtagacg gcaatcataa
     9661 tataagtttg ccaattggca tctacttgtt gacttaatgc tgtccccatt gtaagcatga
     9721 taaacactcc ctttatttaa tttataattt agttacttga aaacattata cataaagata
     9781 aatgtttaga aaagtaggaa aattaaattt a
//
LOCUS       DAMZKC010000098         9543 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.099, whole genome shotgun sequence.
ACCESSION   DAMZKC010000098 DAMZKC010000000
VERSION     DAMZKC010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9543
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.099"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            483..1442
                     /locus_tag="POB55_001772"
     CDS             483..1442
                     /locus_tag="POB55_001772"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDI6500734.1"
                     /translation="MKTFGKKVVLIGDGSVGSSYAFAMVTQGVADEFVIIDIAKDKVK
                     ADVQDLNHGTVHSPSPVDVKAGEYEDCKDADLVVITAGAPQKPGETRLQLVEKNTKIM
                     KSIVKSVMDSGFDGYFLIAANPVDILTRFVKEYTGLPAERVIGSGTVLDSARLQYLIS
                     QELGVAPSSVDASIIGEHGDTELAVWSQANVAGISVYDTLKEQTGSEAKAEEIYVNTR
                     DAAYEIIQAKGSTYYGIALALMRISKAILNNENNVLNVSIQLDGQYGGHKGVYLGVPT
                     LVNQHGAVKIYEMPLSAEEQALFDKSVKTLEDTFDSIKYLLED"
     gene            1720..2424
                     /gene="budA"
                     /locus_tag="POB55_001773"
     CDS             1720..2424
                     /gene="budA"
                     /locus_tag="POB55_001773"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HDI6500735.1"
                     /translation="MTNVLYQHGTLGTLMAGLLEGTATINELLEHGNLGIATLTGSDG
                     EVIFLDGKAYHANEHKEFIELKGDEKVPYASITNFKASKTFSLQQLSQDDVFAQIKNE
                     MLSENLFSAVKIYGTFKHMHVRMMPAQQPPYTRLIDSARRQPEEKRQNIRGAIVGFFT
                     PELFHGVGSAGFHIHFADDERAYGGHVLDFEVDDVVVEIQNFETFQQHFPVNNETFVK
                     AKIDYKDVAEEIREAE"
     gene            complement(2510..3370)
                     /locus_tag="POB55_001774"
     CDS             complement(2510..3370)
                     /locus_tag="POB55_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDI6500736.1"
                     /translation="MLSVAIIGPGAVGTTIAYELQQSLPHTTLIGRHAKTITYYTVPH
                     APAQDIVVKGYEDVTNTFDVIIIAVKTHQLDAVIPHLTHLAHEDTLIILAQNGYGQLE
                     HISFKNVCQAVVYISGQKKGDVVTHFRDYQLRIQDNALTRQFRDLVQDSQIDIVLEAN
                     IQQAIWYKLLVNLGINSITALGRQTVAIMHNPEIRTLCRQLLLDGCRVAQAEGLNFSE
                     QTVDTIMTIYQGYPDEMGTSMYYDIVHQQPLEVEAIQGFIYRRAREHNLDTPYLDTIY
                     SFLRAYQQNM"
     gene            3443..4261
                     /gene="panB"
                     /locus_tag="POB55_001775"
     CDS             3443..4261
                     /gene="panB"
                     /locus_tag="POB55_001775"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HDI6500737.1"
                     /translation="MKTVSQLIDMKQKQTKISMVTAYDFPSAKQVEAAGIDMILVGDS
                     LGMTVLGYESTVQVTLADMIHHGRAVRRGAPNTFVVVDMPIGAVGISMTQDLNHALKL
                     YQETNANAIKAEGAHITPFIEKATAIGIPVVAHLGLTPQSVGVMGYKLQGATKEAAEQ
                     LILDAKNVEQAGAVALVLEAIPNDLAEEISKHLTIPVIGIGAGKGTDGQVLVYHDMLN
                     YGVEHKAKFVKQFADFSVGVDGLKQYDQEVKSGAFPSEEYTYKKKIMNEVNNND"
     gene            4254..5105
                     /locus_tag="POB55_001776"
     CDS             4254..5105
                     /locus_tag="POB55_001776"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HDI6500738.1"
                     /translation="MTKLITTVKEMQHIVKAAKRSGTTIGFIPTMGALHDGHLTMVRE
                     SVSTNDITVVSVFVNPLQFGPNEDFDAYPRQIDKDLELVSEVGADIVFHPAVEDMYPG
                     ELGIDVKVGPLADVLEGAKRPGHFDGVVTVVNKLFNIVMPDYAYFGKKDAQQLAIVEQ
                     MVKDFNHAVEIIGIDIVREADGLAKSSRNVYLTEQERQEAVHLSKSLLLAQALYQDGE
                     RQSKVIIDRVTEYLESHISGRIEEVAVYSYPQLVEQHEITGRIFISLAVKFSKARLID
                     NIIIGAE"
     gene            5107..5490
                     /locus_tag="POB55_001777"
     CDS             5107..5490
                     /locus_tag="POB55_001777"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015901315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HDI6500739.1"
                     /translation="MIRTMMNAKIHRARVTESNLNYVGSITIDSDILEAVDILPNEKV
                     AIVNNNNGARFETYVIAGERGSGKICLNGAASRLVEVGDVVIIMTYAQLNEEEIQNHA
                     PKVAVMNEDNVIIEMIHEKENTIVL"
     gene            5611..6207
                     /locus_tag="POB55_001778"
     CDS             5611..6207
                     /locus_tag="POB55_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDI6500740.1"
                     /translation="MLIFTDNANAENNKPEGVNENSNLIPVRQPDANYPGPVSDIARV
                     KKLDNLIDGTKDPDVIKQLKDDNEVPNNAVKAKDSKIVSDCRHLLKHPDNLIDGTKDP
                     NVINQLKNANGTSNNSAVNPSIKANDIHTKSNKQMAKQQSIHLDSKSKSVHKQQLKEL
                     PKTGVNNESNTAIMALFIGLILLVVSLVTRFIKLNSHQ"
     gene            complement(6305..7987)
                     /locus_tag="POB55_001779"
     CDS             complement(6305..7987)
                     /locus_tag="POB55_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HDI6500741.1"
                     /translation="MNQHLLGNPKLTVTHVNEVKAGINHIVVDSVQYGNQEMIMEKDV
                     TVEMRDGEKLYINIFRPNKDGKFPVVMSADTYGKDNKPKITNMGALWPTLGAIPTSSF
                     TPEESPDPGFWVPNDYVVVKVALRGSDKSKGVLSPWSKREAEDYYEVIEWAANQSWSN
                     GNIGTNGVSYLAVTQWWVASLNPPHLKAMIPWEGLNDMYREVAFHGGIPDTGFYRFWT
                     QGIFARWTDNPNIEDLIQAQQEHPLFDDFWKQHQVQLSQIKTPLLTCASWSTQGLHNR
                     GSFEGFKQAVSEEKWLYVHGRKEWESYYARENLERQKSFFDFYLKEENNDWKDTPHVI
                     YEVRDQFYKGEFKSASAFPLPNTEYTPLYLNAENHTLNHAKISSAYVAQYDSEDKRQD
                     VSFKYTFDKDTELVGNMNLKLWVSTKDSDDMDLFAGIKKLDRRGNEVNFPDFNHIENG
                     QVATGWLRVSHRELDQEKSTIAQPWHKHETELKLSQDEIVPVEIELLPSGTLFKQGET
                     LEVVVKGSEIVIGNSTPGMKTRYEHEETVNKGMHMIYTGGKYDSQLIIPIVN"
     gene            8044..8152
                     /locus_tag="POB55_001780"
                     /pseudo
     CDS             8044..8152
                     /locus_tag="POB55_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501367.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8130..8717
                     /locus_tag="POB55_001781"
     CDS             8130..8717
                     /locus_tag="POB55_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDI6500742.1"
                     /translation="MTIDRRIRKSQVAMRNAFIELLHQHQLEEITVQQIADLADVNRS
                     TFYTHYYDKYDLLEKLEDQQLEEIRDFIHDEKLNGAVKLSTDNIHQIMTVLIEMIEKD
                     IAFYQLMFRMGKDSNIHEKLYDLIMCHLQRYKNENDQINGIPFSYFMSYVSGAGLSFI
                     RHWVEDPNRIDKVDLIRHFNTIVNQGPATIIKNIQ"
     gene            complement(8798..9247)
                     /locus_tag="POB55_001782"
     CDS             complement(8798..9247)
                     /locus_tag="POB55_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/extradiol
                     dioxygenase family protein"
                     /protein_id="HDI6500743.1"
                     /translation="MTALFPYIAFENSKEAIAYYEEVFGATDVKRLEVGEEQASHFGM
                     TKEEAQEATMHAEFEVLGVKVLCSDSFGRADKINNGISLLIDYDVNNKEDADKVEAFY
                     EQIKDHSSIEIELPFADQFWGGKMGVFTDKYGVRWMLHGQDYTAIQQ"
BASE COUNT         3239 a         1461 c         1818 g         3025 t
ORIGIN      
        1 tttttgctaa ttttctaaca attttgcaca ttatattttt aaaatcaatt tcatgtttat
       61 gtctgattgg ctagtttgta tgaaatgtaa ttctttgact ataaaatgat gcatcaaata
      121 tgatgagggt aaggcggaaa ataggaggtt atatagtaag tcatttaagt cgaatagcca
      181 tcctttttaa aatgaaaaaa atagaaagct caatagtttg ttaaagcctt taaatagcgt
      241 cgttacgcaa ttttagaatg ctaaaaattg tcacaaattt caaattacgt gctaaagctt
      301 gagatatcaa tatttattgg cgatagagtg taatttgact ccgcttacat ctcagtttta
      361 tgtttgtaat ttggtagcat aatattataa taaaataaaa ttgttaatct ttaatttcag
      421 tatagatatt tttacgtgta gtcacgtgta aaataaattc aattaggtta ggagacataa
      481 ttatgaaaac atttggtaaa aaggttgtat taatcggaga tggatctgta ggatcaagct
      541 atgcctttgc aatggttacg caaggtgttg ctgatgaatt tgtaattatt gacattgcaa
      601 aagacaaagt aaaagcagat gttcaagatt taaaccatgg tacagtccac agtccttcac
      661 cagttgatgt gaaagcaggt gaatacgaag actgtaaaga tgcagattta gttgttatta
      721 cagctggtgc acctcaaaag ccaggtgaaa cacgtttaca attagttgaa aaaaatacta
      781 agattatgaa gagcatcgtt aagagtgtta tggatagtgg ctttgatgga tatttcttaa
      841 tcgcggcaaa ccctgtagac attttaacaa gatttgtaaa agaatatact ggattaccag
      901 cagagcgtgt tatcggttca ggtactgtat tggacagtgc acgtttacaa tatttaatta
      961 gccaagaact tggtgttgca ccttcaagtg ttgacgctag tattattggt gagcatggtg
     1021 atactgaact tgcagtttgg tcacaagcaa atgtagcagg tatttcagta tatgacacat
     1081 taaaagaaca aactggtagc gaagctaaag cggaagaaat ttatgtaaat acacgtgacg
     1141 ctgcttatga aattatccaa gctaaagggt caacatacta tggtattgca ttagcattga
     1201 tgcgcatttc aaaagccatt ttaaataatg aaaataatgt cttaaatgtt tctatacaat
     1261 tagatggtca atatggtggt cacaaaggcg tttacctagg tgtaccaaca ttagttaacc
     1321 aacatggcgc agttaaaatt tatgaaatgc cattaagtgc cgaagaacaa gcgttgttcg
     1381 ataaatctgt taaaacatta gaagatacat ttgattcaat taaatattta ttagaagact
     1441 aagcctattt taagtattaa ttagaaatat attaatggta agaggatcta ttagtgttgc
     1501 agtaacacgt ggcactgata ggtccatttt tttagcaagc attgatagag gcaatacttt
     1561 tgcgtaaaat aataattgaa taaagtatta tgactcgtga aaatttagtg acatgaataa
     1621 aaatgttgag gcgtcattga gtataaagga aagtagttct gcattaatca cgaagtagag
     1681 catgacaacg aggaataact atagggagat ggttttggaa tgacgaatgt cttgtatcaa
     1741 catggtacat taggtacgtt aatggctggc ttactagaag gcacagctac aattaatgaa
     1801 ttgttagaac atgggaattt agggattgca acgttaacag ggtctgatgg cgaagtaata
     1861 tttttagacg ggaaggcata tcatgctaac gagcataaag aatttataga attaaaaggc
     1921 gatgagaaag taccgtatgc atcgattact aattttaaag cgagtaagac attttcattg
     1981 caacaattat cacaagatga tgtattcgca caaattaaaa atgaaatgtt aagtgagaat
     2041 ttattttcgg ctgttaaaat ttatggcaca tttaaacata tgcatgtacg aatgatgcct
     2101 gctcagcaac cgccatatac acgtttgatt gattcagcac gcagacaacc tgaggaaaaa
     2161 agacaaaata ttcgtggtgc cattgttgga ttttttacac cagaattatt tcatggcgta
     2221 ggatctgctg gttttcatat acattttgca gatgatgaaa gagcttatgg tggacatgtt
     2281 cttgactttg aagtggatga cgttgtcgtt gagatacaaa actttgaaac attccaacaa
     2341 catttcccgg taaataacga gacgtttgtt aaagcgaaaa tagactataa agatgtggca
     2401 gaagaaatta gagaagctga atagtctaat ataattaaaa gaccttagcg atataaggaa
     2461 caggtggttc taaatgcatc gttaaggtct ttttatatta tatatgagct tacatatttt
     2521 gttgatacgc gcgtaaaaag ctataaatag tatcaagata tggggtgtca agattgtgtt
     2581 cacgtgcacg tcgataaata aagccttgaa ttgcttcgac ttcaagaggt tgctgatgca
     2641 caatgtcgta atacatgctc gttcccattt cgtcgggata accttgatat atagtcataa
     2701 tagtatcgac agtttgttct gaaaagttta acccctctgc ctgtgcaaca cgacaaccat
     2761 ctagtaatag ctgtctacac agtgtacgta tttcaggatt atgcatgatt gcaactgtct
     2821 gtctaccgag tgctgtgata gaattaatgc ccaagttaac taacaattta taccaaatag
     2881 cttgttgaat atttgcttct aatacaatgt cgatttgact gtcttggacc aaatctctaa
     2941 attgtcgagt taatgcatta tcttgtatac gtaattgata atctctgaag tgcgtaacaa
     3001 catcgccttt cttttgacca cttatataga caactgcttg gcatacgttt ttaaatgaaa
     3061 tatgttcgag ttgaccataa ccattttggg ctaaaatgat gagcgtgtcc tcatgtgcaa
     3121 gatgagttaa atgaggaata actgcatcaa gttgatgtgt tttgactgca ataataataa
     3181 catcaaatgt gtttgtgaca tcttcataac ctttcacaac aatatcttgt gcaggtgcat
     3241 gtggtacagt ataatatgtt attgttttgg cgtgtctccc gataagcgtt gtatgaggca
     3301 atgattgttg taattcatag gcaatagttg taccgacagc gcctgggccg ataatcgcaa
     3361 ctgataacat cttcttcttt cctttctata aaagattcat tatactattt ctaataatac
     3421 tgcaaaaaga ggttgaataa cattgaaaac agtaagtcaa ttaatagata tgaaacaaaa
     3481 gcaaactaaa atttctatgg taacagctta tgattttcca agtgctaaac aagtggaagc
     3541 agcgggtatt gatatgattc ttgttgggga ttcacttggt atgactgtat taggttatga
     3601 aagtaccgta caagtgacgt tagcagatat gattcatcac ggtcgtgcag taagaagagg
     3661 cgcaccaaat acatttgtag ttgttgatat gccgattggt gcagtaggca tctctatgac
     3721 acaagactta aatcatgctc taaagcttta tcaggagacg aatgcaaatg caattaaagc
     3781 agaaggagcg catattacac catttattga aaaagcaacg gcaattggta ttcctgttgt
     3841 tgcacattta ggattaacac ctcaaagtgt tggcgttatg gggtataaat tacaaggtgc
     3901 aacgaaagaa gcagcggagc aacttatttt agatgcgaaa aatgtagaac aagctggtgc
     3961 agttgcgtta gtacttgaag caattccaaa tgatttagca gaagaaatta gcaagcactt
     4021 aacaattcca gtcattggta ttggtgcagg aaaaggtaca gatggtcaag tattggttta
     4081 tcacgatatg ttaaattatg gtgttgaaca taaagcgaaa tttgtgaagc aatttgctga
     4141 ttttagtgtt ggtgtggatg gtttaaaaca atacgatcaa gaagttaaat ctggtgcatt
     4201 tccatcagaa gaatatacgt ataaaaagaa aattatgaat gaggttaata acaatgacta
     4261 agctgattac tacggtaaaa gagatgcaac acattgttaa agcagccaaa cgctcaggaa
     4321 caacgattgg ttttatccca acaatgggtg ccttacatga tggacattta acgatggtac
     4381 gcgaatcagt tagtacaaat gatattacag ttgtgagtgt atttgttaat ccattacaat
     4441 ttggtccaaa cgaagatttc gatgcttatc cgagacaaat tgataaagat ttagaattag
     4501 tatcggaagt aggggcggac attgtttttc atcctgcagt agaagatatg tatccaggtg
     4561 aattaggtat tgatgtcaaa gtaggtccat tagctgatgt gttagaagga gcgaagcgtc
     4621 cagggcattt tgatggggtg gtaacagtag ttaataagct atttaatatt gttatgccgg
     4681 attatgctta ttttggtaaa aaagatgccc agcaattggc tattgtcgag caaatggtaa
     4741 aagacttcaa tcatgccgtt gaaattattg gtattgatat cgttcgagaa gcagatggtt
     4801 tggcgaaaag ttcaagaaat gtttatttaa cagagcaaga acgacaagaa gcggtacatt
     4861 taagtaaaag tttgctatta gcacaagcgt tgtatcaaga cggtgaacgt caaagtaaag
     4921 taattataga cagggttact gaatatcttg aatcacatat aagtggacgc attgaagaag
     4981 ttgctgttta tagttatcct caattagtag aacaacacga aataactgga cgcatattta
     5041 tctcgttagc tgttaaattt tcaaaagcgc gtttaataga taatataata attggagctg
     5101 aataaagtga taagaacaat gatgaatgct aaaattcata gagcaagagt gactgagtca
     5161 aatttaaatt atgtaggtag cattacgatt gattcagata tattggaagc ggtagacatc
     5221 ttgccaaatg aaaaagtagc catcgtgaat aataataatg gtgcacgttt tgaaacatac
     5281 gtaatagctg gtgaaagagg tagtggtaaa atttgtttaa acggtgcagc atcaagactt
     5341 gttgaagtag gcgatgtagt cataatcatg acttatgcac agttgaatga agaagaaatt
     5401 caaaaccatg cacctaaagt agcagtaatg aatgaagaca atgtcattat tgaaatgatt
     5461 catgaaaaag aaaatacgat agtgttataa aatagtataa gttagaaacc tctagtagat
     5521 aacaatgtag ttatccatta ggggttttta tttttatgcc aattgggtta gcgtaataga
     5581 agtaaaggta ttatcgttgc aattttatca gtgttgattt ttacggataa tgcgaacgcc
     5641 gaaaataaca agcctgaagg tgtgaatgaa aacagcaatt taataccagt tagacaaccg
     5701 gatgcaaatt atcctgggcc agttagtgac atagcacgtg tgaaaaaact tgataattta
     5761 atagatggca cgaaagatcc agatgttatc aagcagttga aagatgataa tgaggtgccg
     5821 aataatgcgg taaaggcaaa agattctaaa atagtatccg actgcagaca tttactaaaa
     5881 catccagata atttaataga tggcacgaaa gatccaaatg ttattaatca gttgaaaaac
     5941 gcaaatggaa catcgaataa tagtgctgta aatccaagta ttaaagcaaa tgatattcac
     6001 acaaaaagta ataaacaaat ggctaaacaa caaagtattc atttggatag caagagcaag
     6061 tcagtacata agcaacaatt gaaagaattg ccaaaaacag gagtaaacaa cgaaagtaat
     6121 acagcaataa tggctttatt tattggttta atattgcttg tagtaagttt agtgacaaga
     6181 tttataaaat taaatagcca tcaataataa aaagggccac accttggttt aagattccaa
     6241 gcgtgtggcc ttctcttcta tcactatgtc acttttaatc aaaagcgacc gtaattgctg
     6301 catatcaatt aacgatagga atgattaatt gtgaatcata tttaccacca gtataaatca
     6361 tgtgcatgcc tttatttacg gtttcttcat gttcataacg cgttttcatg ccaggggtac
     6421 tattaccaat tacaatttca ctacctttta caacaacttc caatgtttcg ccttgtttaa
     6481 atagcgtgcc tgaaggtaac aattcgattt caacaggtac aatctcatct tgtgacaact
     6541 ttaattctgt ttcatgttta tgccaaggtt gcgcaattgt ggatttttct tgatctaatt
     6601 cacgatgtga tacgcgtaac caaccggtag ctacttgacc attttcaata tgattaaaat
     6661 cagggaagtt aacttcatta ccacgacgat ctaacttttt aatacctgca aataaatcca
     6721 tatcgtctga gtctttagtg cttacccata gttttaagtt catgtttcca actaactcag
     6781 tatctttgtc aaacgtatat ttaaaactta catcttgtcg tttatcttca gagtcatatt
     6841 gtgcgacata cgcgctacta atctttgcat gattcaatgt gtgattttca gcattcaaat
     6901 acaatggtgt atattctgtg ttaggtaaag ggaacgctga cgctgatttg aattcgcctt
     6961 tataaaattg atctctaact tcataaatga catgaggcgt atctttccaa tcgttatttt
     7021 cttctttaag gtaaaaatca aagaatgatt tttggcgttc gagattttct ctagcgtagt
     7081 aactttccca ctctttacgt ccatgcacat atagccattt ttcttctgat acagcttgtt
     7141 taaatccttc aaaagagcca cggttgtgca aaccttgtgt agaccaacta gcacatgtta
     7201 gtagaggtgt tttaatttgt gataattgca cttgatgctg tttccaaaaa tcatcgaaca
     7261 aagggtgttc ttgttgtgct tgaatcaagt cttcgatatt tggattatct gtccatctcg
     7321 caaaaatacc ttgagtccag aaacgataaa agccagtatc cggtatacct ccatgaaagg
     7381 ctacttctct atacatatca tttaagcctt cccaaggaat cattgctttt aaatgtggtg
     7441 gatttaatga tgcgacccac cattgagtca ccgcaagata agaaacacca tttgtcccga
     7501 tatttccatt actccatgac tgatttgctg cccattcaat cacttcgtaa taatcttccg
     7561 cttctctttt tgaccatgga gataagacgc cttttgattt gtcactaccg cgtaatgcaa
     7621 ctttaactac aacataatca tttggcaccc aaaatcctgg gtctggtgat tcttcaggtg
     7681 taaaactaga tgtcggaatg gcgcctaatg ttggccaaag ggcacccata ttggtgattt
     7741 taggcttatt atctttacca taagtatctg cagacataac tacagggaat ttgccatctt
     7801 tatttggtct gaaaatatta atatataatt tttcgccatc gcgcatttcc acagtgacat
     7861 ctttttccat aatcatttct tgatttccat attgaacact gtcgacaacg atgtggttaa
     7921 taccggcttt cacttcattg acatgagtta cagttaattt tggatttcct agtaaatgtt
     7981 ggttcattgt tatcaccttt ttctgtttgt ttgtatcacc tatattaaac aggtaaacat
     8041 tgtgtgccaa tcatcaatgt tggagatttg taggataatg aaaataacat ttataattgt
     8101 ttataatcat tggggaggac ttgatacaaa tgactataga tcgacgtatt agaaaatcac
     8161 aagttgcaat gagaaatgca tttattgaac ttttacatca acaccaatta gaagagatta
     8221 ctgtgcaaca aattgcagat ttagctgatg ttaaccgtag tacattttat acgcattatt
     8281 atgataagta tgatttgctt gaaaaattag aggaccagca gttggaggaa ataagagact
     8341 tcattcatga tgagaagtta aatggagcag ttaagttatc cacggataac atacatcaaa
     8401 ttatgaccgt tttaatagaa atgatagaaa aagacatcgc attttatcaa ttgatgtttc
     8461 gtatgggtaa agattcaaat atccacgaaa aactttatga tttaattatg tgtcatttac
     8521 aacgttataa aaatgaaaat gatcaaatta atggtatacc attttcttat ttcatgagtt
     8581 atgtttcagg tgcgggattg tcttttataa gacattgggt agaagatcca aaccgtatag
     8641 acaaagttga cttaataagg cattttaata cgattgtcaa tcaagggcca gcaacgataa
     8701 ttaaaaatat acaataaaat aaggcaagcc atgaatcgag agtggcttgc cttgctaaat
     8761 aacataagct tattaaatga tatctttatc aataacatta ttgttggatt gcagtatagt
     8821 cttgaccatg taacatccaa cgaacaccgt atttatcggt aaagacgccc attttgccac
     8881 cccagaattg gtcagcaaac ggtaattcta tttcaattga agaatgatct ttaatttgct
     8941 catagaatgc ttcaacttta tcagcatctt ccttattgtt aacatcataa tcaattaata
     9001 atgatatgcc attattaatt ttgtcagcgc gaccaaaaga atcagaacat agcactttta
     9061 cgccaagcac ttcaaactca gcatgcatag ttgcttcttg cgcttcttcc ttagtcatgc
     9121 caaaatgtga cgcttgttct tcgccaactt ctaaacgttt aacgtcagtt gcaccaaata
     9181 cttcttcgta atatgcaatg gcttcttttg aattttcaaa agcgatataa gggaataatg
     9241 ctgtcatata aaacacctca atgtattatt tttattacta acaccatgtt acattaaaaa
     9301 atggcaaaag tataactttt acgatgaatt gaagtcaatc ttttgtaaaa tacacgataa
     9361 attataagat gaaattgatt taaatgaagt aaacatatat atgacataca aaaaatgaaa
     9421 atgacacaga gacgtatgtg ttgtttagtg ttttaaaatt caagtttaaa aaatgctttt
     9481 agatacataa gggattttcg aaatataaat gcattaaaat gaatttctaa cttttttgac
     9541 tga
//
LOCUS       DAMZKC010000099         9473 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.100, whole genome shotgun sequence.
ACCESSION   DAMZKC010000099 DAMZKC010000000
VERSION     DAMZKC010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9473
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.100"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            36..809
                     /locus_tag="POB55_001783"
     CDS             36..809
                     /locus_tag="POB55_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDI6500744.1"
                     /translation="MKAHKIFWLNLAAIIIISIVVSGGMFLAMKWEQIHLKDGLKKVL
                     STYPIKNLETLYEIDGHDNPHYENNDQDTWYIESSYSVVGSDELLKEDRMLLKVDKNT
                     HKITGEYDTTTNDRKNATDSTYKSYPVKVVNNKIVFTKDVKDPALKQKIENNQFLIQS
                     GDLTSILNSNDLKVTHDPTTDYYNLSGKLSNDNPNVKQLKRRYNIPRNASTKVELKGM
                     SDLKGNNHQDQKLYFYFSSPGKDQIIYKESLTYNKLSEH"
     gene            complement(1122..3128)
                     /gene="ggt"
                     /locus_tag="POB55_001784"
     CDS             complement(1122..3128)
                     /gene="ggt"
                     /locus_tag="POB55_001784"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HDI6500745.1"
                     /translation="MVINLNDKQTKTSKEGLISVSHPLAAKIGKDVLDQGGNAMDAVI
                     AIQLALNVVEPFASGIGGGGYLLYYEQSTGSITAFDARETAPAHVDKQFYLDDSGEYK
                     SFFDMTTHGKTVAVPAIPKLFDYIHKRYAKLSLEDLINPAIELAIEGHAANWATEKYS
                     RQQHARLTKYYETAQVFTHENQYWREGDWIVQPELGKTFQILREQGFNAFYKGDIAKQ
                     LVNVVKACGGTITLEDLAKYDIQIKAPISATFKDYDIYSMGPSSSGGITVIQILKLLE
                     HVDLPSMGPRSVDYLHHLIQAMHLAYSDRAQYLADDNFHEVPVQSLIDDNYLKARSTL
                     IDSNKANIDIEHGVVSDCISHTDVEENHTETTHFCVIDKEGNIASFTTSIGMIYGSGI
                     TIPGYGVLLNTTMDGFDVVDGGINEIAPYKRPLSNMAPTIVMYHGKPILTVGAPGAIS
                     IIASVAQTLINVLVFGMDIQQAIDEPRVYSSHPNRIEWEPQFSQSTILALIARGHAME
                     HKPDAYIGDVHGLHVDLNTRDASGGADDTREGTVMGGEVLSIRKQPLLSPEIYDNDTH
                     RVYFNDVQLPLLADQVRWMHDKYWVDESVVRIIFSEVSAHIEDLRSYENAGENYIDIA
                     WLARKKGYQVALKDDGLYLTDDTYTSVKRNTNAYYRYDRDSITR"
     gene            complement(3166..4941)
                     /locus_tag="POB55_001785"
     CDS             complement(3166..4941)
                     /locus_tag="POB55_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDI6500746.1"
                     /translation="MKKIISIAIIVLALVLSGCGVPTKSEVAQKSSKVEVKGERPTIH
                     FLGQASYENDMNIVKDQLENAGFNVKMNIQPDYGSYRTQRQAGNYDIQIDDWMTVFGD
                     PNYAMTALFSSTGSNSLLKDKHVDQLLNKASTQNEADVKQTYKQIEDEVVFDKGYMAP
                     LYGSKKNLVYDNKVLDKNSVGLPNSRALIWQQFDYNNSRERDTRPLVMTQQDGEIPTL
                     DPIRSIAPSVYSINMNMYTRLLLLDENDHLTTKGSLSHDYAVNKDNKAFYFLLRDDDY
                     FAKVVNGQARNTGERVSAEDVKFSLDRARDKKSVPNNNTYNMHKHINDIKILKDEDID
                     QLRKEKDKDDKSIYDKLIKAYNVKSLTTDGQKVNNKDGIYQIVKITTDQSMPREVNYL
                     THSSAGILSKKFVNQVNQEYPKGYGDSSTIPANSDGKNALYASGAYIMTQKNAYQATF
                     QRNPGFNETEKGSYGPAKIKNITLKFNGDPNNALSELRNHSIDMLADVNQKHFDLIKS
                     DKNLSIIRKNGRKSVFLMLNIKKGIFKTHPNLRQAVVNAIDQDQFIKFYRGDKFKIAS
                     PITPLVDTGNEQRQDLEKVEKAINQ"
     gene            complement(4958..6121)
                     /locus_tag="POB55_001786"
     CDS             complement(4958..6121)
                     /locus_tag="POB55_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6500747.1"
                     /translation="MVKLTTKIASLKLFASYAIATYILVILTSALNLFKGYVADTFYI
                     AETLLIVLTIILIIILTTEQTWKHHDLWRRIVEVLLLLMTLTGNVFTLLMFVSIRRYQ
                     RTSQIHSYNGWESFIRKTTRHRIAIIGLLILVYMLTLSIVSQFTFDTTLATKNQFNAL
                     LHGPSLAYPFGTDDFGRDLFTRVVVGTKLTFSISIISVVIAVIFGVLLGTIAGYFNHI
                     DNLIMRILDVVFAIPSLLLAVAIIASFGASIPNLIIALSIGNIPSFARTMRASVLEIK
                     RMEYVDAARITGENTWNIIWRYILPNAIAPMIVRFSLNIGVVVLTTSSLSFLGLGVAP
                     DVAEWGNILRTGSNYLETHSNLAIVPGVCIMFVVLAFNFIGDAVRDALDPRIH"
     gene            complement(6127..7605)
                     /locus_tag="POB55_001787"
     CDS             complement(6127..7605)
                     /locus_tag="POB55_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6500748.1"
                     /translation="MNCTFRLLTSNKFDMMLCLPIHKCVNLFYTFVYHSNLLKIILQG
                     VIIIITYLESFKSLYHKAFYKFVLSVLSLPIFLYAVIKFFFSAKRKNFYANNSEISEI
                     ERALHQKYKYLSQQKSSTQIHKEALKIFKAQSSNTSSKNIEQAHFSTYFENLLFHKFI
                     MIKVILALPMFILLTIYLQPLVRYIFERIVMAVIVIIGVIVSVFTILYFSPLDAAYSI
                     LGQNATKAQIHQFNVLHHLNEPYFIQLWDTIKGVFTFDLGTTYKGNEVVTKAVGERIP
                     ITIIVAVLALIVALIIAIPIGIISAMKRNSWLDITLMIIALIGLSIPSFWQGLLFILA
                     FSLKLDILPPSYMPEHPISLILPVLVIGTSIAASITRMTRSSVLEVMRSDYVLTAYAK
                     GLSTTQVVIKHILKNAIIPIVTLVGLLVAELLGGSAVTEQVFNINGIGRYIVQKQLIP
                     DIPAVMGGVVYISIVISLANLIIDIFYALIDPKLRSEINERK"
     gene            7562..9154
                     /locus_tag="POB55_001788"
     CDS             7562..9154
                     /locus_tag="POB55_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6500749.1"
                     /translation="MSNLLEVNSLNVQFNYDETTVQAVKNVSFELRKKHILGIVGESG
                     SGKSITAKSILGLLPDYPDHTLTGEIIFNGQSLNNLSTSALQQIRGKDISMIFQDPLS
                     SLNPRLTIGKQITEVLFQHKRVSKSEAKSMTIDILEKVGIKHATRQFDAYPHELSGGM
                     RQRVMIAMALILKPQILIADEPTTALDASTQNQLLQLMKSLYEYTETSIIFITHDLGA
                     VYQFCDDVIVMKDGSVVESGTVESIFKSPQHTYTKRLIDAIPDIHQTRPPRPLNNDIL
                     LKFDRVSVDYTSPSGSLYRAVNDINLGIRKGETLGIVGESGSGKSTLAKTVVGLKEVS
                     EGFIWYNELPLSLFKDDELKSLRQEIQMIFQDPFASINPRFKVIDVIKRPLIIHGKVK
                     DNDDIIKTVVSLLEKVGLDQSFLYRYPHELSGGQRQRVSIARALAVEPKVIVCDEAVS
                     ALDVSIQKDIIELLKQLQLDFGITYLFITHDMGVINEICDRVAVMKNGEIVELNNTED
                     IIKHPQSDYAKQLISEVAVIAK"
BASE COUNT         3114 a         1764 c         1496 g         3099 t
ORIGIN      
        1 tgttaattaa gtattttaag aaaaggtgtg aatgtatgaa agcacataaa attttctggt
       61 taaacttagc agcaattatt attatttcaa ttgtagtcag tggcggtatg tttttggcaa
      121 tgaagtggga acaaattcat ttgaaagatg gtcttaaaaa agtactaagt acgtatccta
      181 ttaaaaactt agaaacgctt tatgaaattg atggtcacga taatccccat tatgaaaata
      241 atgatcaaga cacatggtat atagaatcct cttattcagt tgttggatca gatgagcttt
      301 taaaagaaga ccgtatgttg ttaaaggtag ataagaacac acataaaata actggtgaat
      361 atgacacaac tacaaacgat agaaaaaatg ctactgactc gacatacaaa agttatccag
      421 taaaagtagt taataataaa atcgttttca caaaagacgt aaaagatccc gcgctgaaac
      481 aaaaaatcga aaacaatcaa tttttgattc aaagtggtga tttaacaagc attttaaata
      541 gcaatgattt gaaagtcaca catgatccta ccactgatta ttataattta tctggtaagt
      601 tgtcgaatga taatccaaac gttaaacaat taaaacgtag atataatatt cccagaaatg
      661 catcaacaaa ggtggaatta aagggaatga gtgatttaaa aggcaataat catcaagatc
      721 agaaacttta tttttatttt tcaagtcctg gaaaagacca aattatatac aaagaaagcc
      781 ttacttataa taaattaagt gaacattaat acttatgatg taattataga aataaccaaa
      841 tcatttatta tcgtttaaca tactaaaatc acacattata aaaacgcctc acatcattca
      901 tttaattgta ccgaatcaca tcaagttaga ttttagacta actattgtgg tgtacatcaa
      961 aaacgaattt gtggggcgtt ttatatttta caaaggcgac tagcgtttct tataacaact
     1021 tcaacattac tacactcaag cgtcacgcta aagttcaaaa tcgaatcctt tcattcaaca
     1081 aaagccctta tccactacaa acttcatatc aagcgtataa actatcttgt gatactatct
     1141 cgatcatatc tatagtatgc atttgtgttc cgtttcactg aagtatatgt atcatcagtt
     1201 aagtataaac catcatcttt caatgccact tgataacctt tctttcgtgc taaccaggca
     1261 atatctatat aattttctcc tgcgttttca taacttctta aatcttcaat atgtgcacta
     1321 acttcagaga agatgattct aacgacactt tcatcaaccc aatatttgtc atgcatccat
     1381 cgcacttgat ctgccaataa aggtaactgc acatcattga aatatacacg atgggtgtca
     1441 ttatcgtata tttcgggact aagtaatggt tgttttctaa ttgataatac ctcaccaccc
     1501 atcactgtac cttccctcgt atcatcagca cctcccgatg cgtcacgtgt attcaagtca
     1561 acatgtagtc catgtacatc tccaatatag gcatctggtt tatgttccat tgcatgtcca
     1621 cgtgcaatca atgctaatat tgtagattgt gaaaattgag gctcccattc aatgcgatta
     1681 ggatggctac tataaactct aggttcatct atagcctgct gaatatccat gccaaacact
     1741 aatacattga ttaatgtttg cgcaacacta gcaatgatac ttatggcacc aggtgcacct
     1801 actgttaata ttggcttccc gtgatacatc acaatcgttg gagccatgtt acttagtggt
     1861 cgtttatatg gtgcaatttc gttaatacca ccatctacta catcaaagcc atccattgtc
     1921 gtattcaata acacgccgta gcctggaatc gtgatacccg atccgtaaat cataccgatt
     1981 gatgtcgtaa atgaagcaat attaccttcc ttatcaatca cacaaaaatg agttgtttcg
     2041 gtatgatttt cttcaacatc tgtatgacta atgcaatcag acacaacacc atgctctata
     2101 tcaatatttg ctttattgct atcaatgagc gtactgcgtg cttttaaata attgtcatca
     2161 attaatgact gtacaggcac ctcatgaaaa ttatcatccg ccaaatattg cgcacgatca
     2221 ctatatgcta aatgcatcgc ttgtatcaaa tgatgcaagt aatcaacaga tcttggaccc
     2281 atagatggta aatcgacatg ttctaataac ttcaatattt gaattaccgt gataccgcca
     2341 gaactagatg gtcccattga ataaatgtca tagtctttaa atgttgcact gattggcgct
     2401 ttaatctgaa tgtcatattt ggctagatcc tctaaagtga ttgtcccacc acatgctttg
     2461 acaacattga ctaattgttt cgcaatgtca cctttataaa atgcattaaa cccttgttct
     2521 cttaatattt gaaatgtctt acctaattcg ggttgtacaa tccaatcacc ttcacgccaa
     2581 tattgatttt catgcgtaaa tacttgtgcc gtttcataat actttgtcaa tcgtgcgtgt
     2641 tgctggcgcg aatatttttc agtagcccaa ttggctgcat gaccttcaat agctagttca
     2701 attgcaggat taattaaatc ttccaatgac aacttagcat aacgcttgtg aatataatca
     2761 aacagctttg gaattgctgg cacagcgaca gttttaccat gtgtagtcat atcaaaaaat
     2821 gatttatatt cgcctgaatc atctagataa aattgtttat ctacatgtgc aggggctgtc
     2881 tcacgcgcat caaacgcagt tatactgcca gtactttgct cataatatag caaatacccg
     2941 ccaccaccaa tacctgatgc aaatggttct accacattca acgccagttg aattgcaatc
     3001 actgcatcca tggcgttgcc accttgatct aatacatcct taccaatttt agccgcaaga
     3061 ggatgtgata cggaaattaa cccttcttta gatgtttttg tctgtttgtc atttaagtta
     3121 atgaccatac tatatcctcc tactttctgt taaatattta aaacattatt gattgatggc
     3181 tttttctact ttttctaaat cttgacgttg ctcgttacca gtatcgacaa gtggtgtaat
     3241 cggtgatgca attttaaatt tatcgccacg ataaaactta ataaattgat cctgatctat
     3301 cgcattaact actgcttgtc tcaagtttgg atgcgtctta aatatacctt ttttaatatt
     3361 tagcattaaa aagactgact tgcgtccatt tttgcgaata atgcttaaat ttttatccga
     3421 cttaattaaa tcaaaatgtt tttgattcac atctgccaac atatcaattg aatgatttct
     3481 aagttctgac aatgcattat tcgggtcacc attaaacttc aatgtaatat ttttaatttt
     3541 agctggtcca taactacctt tttctgtttc gttgaatcct ggattacgtt gaaacgttgc
     3601 ttgatatgca tttttctgtg tcataatgta tgcgccactt gcatacagcg catttttccc
     3661 atctgaattt gcaggaattg tactgctatc cccatatcct tttggatatt cttgatttac
     3721 ttgattaaca aattttttag ataaaatgcc tgccgaagag tgtgttaagt aatttacctc
     3781 tcgaggcatc gattgatctg tcgtaatttt aacaatttga taaataccgt ctttattatt
     3841 tactttttga ccatctgtcg ttaacgattt gacgttataa gctttaatca acttatcata
     3901 gattgattta tcgtccttgt ctttctcttt acgcaactga tcgatgtcct catcttttaa
     3961 tatcttgatg tcatttatat gtttgtgcat attgtaagta ttattgttag gcacagactt
     4021 tttatcacgt gctctatcta aagaaaactt aacatcttca gccgatacac gctctccagt
     4081 attacgtgct tgtccattga ccactttcgc aaaataatca tcatctctta acaagaaata
     4141 aaatgcttta ttgtccttat tcacagcata atcatgactt aacgaacctt tcgttgttaa
     4201 atgatcattt tcatctaata ataataacct tgtgtacata ttcatattaa ttgaatatac
     4261 tgacggcgca attgaacgta ttggatccaa tgtaggaatt tcaccatctt gttgtgtcat
     4321 cacaagtggc cgcgtatctc gttctctact attgttgtaa tcaaattgtt gccatattaa
     4381 tgcacgtgaa tttggcaatc caacactatt tttatctaac actttattgt catatactaa
     4441 attctttttt gatccatata aaggcgccat atacccttta tcaaatacaa cttcatcttc
     4501 aatttgctta tatgtttgtt taacatctgc ttcattttga gtagaagctt tatttaacaa
     4561 ctggtctaca tgtttatctt tcaataaact atttgatcct gtagaactaa ataatgccgt
     4621 catagcatag ttcgggtcac caaacactgt catccagtca tcaatttgga tatcataatt
     4681 gccggcttga cgttgtgtac gatagctacc ataatctggt tggatattca tcttcacgtt
     4741 aaatcctgca ttttccaatt gatctttaac gatattcata tcattttcat aacttgcttg
     4801 tcctaggaaa tgtattgttg gtcgctcgcc tttcacttca actttcgatg acttttgagc
     4861 cacttctgat ttcgtaggga caccacaacc acttaatacc aacgctaaaa ctataattgc
     4921 gatactaatg attttcttca catctatccc taccttttta atgaattctt ggatctagtg
     4981 catcacgcac tgcatcacct ataaaattaa atgctaaaac gacgaacata atacaaacac
     5041 caggtacaat agctaaatta ctgtgcgttt ccaagtagtt actaccggta cgtaaaatgt
     5101 tgccccattc agctacatca ggtgcaacac caagtcctag gaaacttaaa ctacttgttg
     5161 ttaatacaac cacacctata tttaatgaaa aacgtacaat cataggcgca atcgcattcg
     5221 gtaaaatata acgccatatg atattccaag tgttttcacc agtgatacgt gctgcatcta
     5281 catattccat gcgtttaatt tctaaaacac tggcacgcat tgtccgtgca aatgatggta
     5341 tattaccgat acttaaagca ataattaaat ttggaatact tgctccaaat gatgcaataa
     5401 ttgccaccgc taacaataat gatggaattg caaacactac atctaaaatt cgcattatta
     5461 aattatcaat atgattaaaa taacctgcga tagtgcctag taacacacca aaaataactg
     5521 caataactac tgaaataatt gaaattgaaa atgtcagctt cgttcctaca actacgcgtg
     5581 taaataagtc tctaccgaaa tcatcagtac caaacggata ggctagactc ggtccatgta
     5641 acagtgcatt gaactgattt ttagtagcca atgtcgtatc aaatgtaaat tgtgacacaa
     5701 ttgataatgt cagcatgtag actaaaataa gtaacccgat aatcgcaata cgatgtctag
     5761 tagtttttcg tataaacgat tcccacccgt tataactatg tatttgcgat gtacgttggt
     5821 aacgtctaat acttacaaac attaataatg taaatacgtt gcctgttaat gtcatcaaca
     5881 ataacaacac ttcgacgata cgtcgccata agtcatgatg cttccatgtt tgttccgttg
     5941 ttaaaatgat aattaaaatg atggttaaaa cgattagcaa tgtttcagca atatagaacg
     6001 tatcggccac ataaccttta aaaagattta atgcactcgt taatataact aaaatataag
     6061 ttgctatagc ataacttgcg aatagtttta aggaagctat ctttgttgta agttttacca
     6121 tatgcctcac ttcctttcgt taatttcact acgtaatttt ggatcgatta aagcataaaa
     6181 tatatcaata attaagtttg ctaaagatat tacaattgat atatatacga ccccacccat
     6241 gactgctggg atatcaggta ttagttgttt ttggacgata taacgcccga taccattaat
     6301 gttaaatact tgttccgtca ctgctgaacc gcctagtaac tctgccacta gaagaccaac
     6361 taacgttaca attggaataa tggcattttt caaaatatgt ttaataacaa cttgtgtcgt
     6421 cgataatcct tttgcataag cagttaaaac ataatcgctg cgcattactt caagtacaga
     6481 agaccttgtc atacgcgtga tagaagcagc aatacttgtt ccaatgacaa gtacaggtaa
     6541 aatcaacgat attggatgtt ctggcatata agatggtggc aaaatatcca atttcaatga
     6601 gaacgctaaa atgaataata gcccttgcca gaaacttgga atagataaac caattaatgc
     6661 aattatcatt aacgtgatat caagccaact atttcgcttc atcgcactga taataccaat
     6721 tggtattgca ataattaatg ccacaattag cgctaatact gcgacaatta ttgtaattgg
     6781 aattctttcg ccaactgctt tagtcacaac ctcattccct ttgtaagtcg tacctaagtc
     6841 aaaggtaaaa acacccttga tggtatccca caattgaata aaataaggtt cgttaagatg
     6901 atgtaataca ttgaattgat gtatctgtgc ctttgttgca ttttgtccca gtatgctata
     6961 agccgcatca agcggtgaaa aatacagaat ggtaaacaca ctgacaataa caccaatgat
     7021 gacaatcaca gccatgacaa ttctctcaaa aatatatcta actaatggct gtaaataaat
     7081 agtcaataag atgaacatcg gcaaggccaa tattactttg atcatgatga acttatgaaa
     7141 taataaattt tcaaagtatg ttgaaaaatg tgcttgttca atattctttg aactcgtatt
     7201 agaactttgt gccttgaata tttttaatgc ttctttatgt atttgtgtgg atgacttttg
     7261 ctgcgataaa tatttatatt tttgatgtaa cgctcgttca atttctgaaa tttcagaatt
     7321 attagcgtaa aaattttttc tcttagcaga aaagaaaaac tttatcactg catataaaaa
     7381 tattggcaag cttaataccg ataatacaaa cttgtaaaat gccttgtgat acaacgactt
     7441 aaaagattct aaataagtaa ttatgataat cactccttgt aaaataatct tgagtagatt
     7501 actatgatat acaaaagtat agaataaatt tacacatttg tgaataggga ggcacaacat
     7561 catgtcaaat ttattagaag tcaacagtct gaatgtacaa ttcaattatg atgaaactac
     7621 agttcaagcg gtaaaaaatg tctctttcga attacgaaaa aaacatatcc taggtattgt
     7681 tggtgaatca ggatcaggaa aaagtattac tgctaaatct attttagggc tactaccaga
     7741 ttatccagat cacacattaa caggagaaat tatttttaat gggcaatcgt taaataattt
     7801 atcaacttca gcgttacaac aaattcgagg taaggatatt tcaatgattt ttcaagatcc
     7861 actctcttcg ttgaatccaa gattaacgat tggcaaacaa attacagaag tactatttca
     7921 acataaacgt gtatctaaat ctgaagcaaa gtcgatgaca atagacattt tagaaaaagt
     7981 aggtataaaa catgcaactc gacaatttga tgcttatcca catgaacttt ctggtggtat
     8041 gcgtcaacgt gtcatgatag caatggcatt gattttaaag ccacaaattt taatcgcaga
     8101 tgaaccaaca acggcattag atgccagtac acaaaatcaa ttactgcagt taatgaagtc
     8161 cctttatgag tacacagaaa catctattat ttttatcact cacgatttag gcgctgtgta
     8221 tcaattttgc gacgatgtga ttgtaatgaa agatggaagt gtcgttgaaa gtggcacggt
     8281 tgaaagtatt tttaaatcgc cacaacatac ctatacaaaa cgcttaatag atgcgattcc
     8341 tgatattcat caaacgcgtc cgccaagacc gttaaacaat gatattttat taaaattcga
     8401 tcgcgtgagc gtggattaca catcaccgag tggcagccta taccgagcag ttaatgatat
     8461 taacttgggt attagaaaag gcgaaacatt aggcattgtc ggtgaatcag ggtcagggaa
     8521 atcgacatta gctaagacgg tcgtcggtct aaaggaagtg tcagaaggct ttatttggta
     8581 taacgaatta ccattaagtt tatttaaaga tgatgaattg aaatctttac gacaagagat
     8641 acaaatgatt tttcaagatc cattcgcatc tattaatcca agatttaaag tcattgatgt
     8701 gattaaacga ccactaatca ttcatgggaa agtcaaagat aatgatgaca ttattaaaac
     8761 tgtcgtatcg ttgttagaaa aggttggcct agatcaaagt ttcttatatc gctatccaca
     8821 cgaattatct ggtgggcaac gtcagcgtgt aagtatcgcg agagcacttg ctgtagaacc
     8881 taaagtgatt gtttgcgacg aggcagtgtc cgctttagac gtttcaattc aaaaagatat
     8941 catcgagtta ttaaaacaat tacagttaga cttcggcatc acttatttat tcatcacaca
     9001 tgacatgggt gttatcaatg aaatatgtga tcgcgttgca gttatgaaaa atggcgaaat
     9061 cgttgaactg aataacacag aagatattat caaacatccg caatcagact atgcaaagca
     9121 acttatttca gaagtagcag ttattgctaa ataaaagtca tgcgttgtgc aactgtatca
     9181 ctgtatggtc tggaataaat caggcaatct ctgatgtgtt atgttagatg actttgtcat
     9241 actaactata ttgtgctaag ttagggctcg gcaccatatc aagttttagg tgctttgcca
     9301 tgatttaaga gtcaccccca tactttgggc attttgacgc cagaataaat cccccgccac
     9361 tatgtgaagt gcgggggatt atttatattt tattagaata ttcagatttt tgagtgtgtc
     9421 aacttagctt agtcaatgta tatttaacgt cacttactct ttttctttca taa
//
LOCUS       DAMZKC010000100         9399 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.101, whole genome shotgun sequence.
ACCESSION   DAMZKC010000100 DAMZKC010000000
VERSION     DAMZKC010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9399
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.101"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            130..765
                     /locus_tag="POB55_001789"
     CDS             130..765
                     /locus_tag="POB55_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HDI6500750.1"
                     /translation="MSDQVKIPRATLKRLPLYYRFVSSLKSKGIDRVNSKAISDALQI
                     DSATIRRDFSYFGELGKKGYGYNIDSLLDFFKSELSESDMIKIAIVGVGNLGKALLTY
                     NFSIHDDMTITEAFDVKEDVIGQKIGNVIVKDNDELITTLKKEEIDVVILTTPERVAQ
                     KVADELVQAGVKGILNFTPGRINTPSDVQVHQIDLGIELQSLLFFMKNYSE"
     gene            1069..2148
                     /locus_tag="POB55_001790"
     CDS             1069..2148
                     /locus_tag="POB55_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HDI6500751.1"
                     /translation="MWMIFSGLIVGGLLGFVMQRTRFCLTGGFRDMYVQKNNKMFYAL
                     LIAITIQSIGLLILTATDILKIPAHSFPILGTIIGSFIFGIGIVLAGGCATGTWYRAG
                     EGLIGSWIALVLYAVTAAITKTGILKPVMDKINQPTNVNSDMSQTTGIPFWGLVVILT
                     IITIFLVVRTLNNKKVRVAVPKLKQRYTGIRYYLFEKRYHPFIAAIVIGLIALLAWPM
                     SASTGRNDGLGITTPSANLVHFLITGETKFIDWGVFLVLGIFIGSYIAARGSREFKWR
                     LPDKITIRNSAIGGICMGFGASVAGGCSIGNGLVETATMTWQGWIALASMIVGVWTMS
                     HFIFVRPMKKVQQQSAKVKQQTQIV"
     gene            2208..2432
                     /locus_tag="POB55_001791"
     CDS             2208..2432
                     /locus_tag="POB55_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HDI6500752.1"
                     /translation="MIHELGTVGMVCPFPLIEAQKKMATLQSGDELKIDFDCTQATEA
                     IPNWAAENGYPVTNYEQIDNASWTITIQKV"
     gene            2640..3890
                     /locus_tag="POB55_001792"
     CDS             2640..3890
                     /locus_tag="POB55_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HDI6500753.1"
                     /translation="MNLNDTIFMFLCTLLVWLMTPGLSLFYGGLVQSKNALNTVMQSM
                     AAIVLVTFVWITVGFTISFGNGNLWFGNWEYTFLNHVGFATQEDISPHIPFALFMLFQ
                     MMFCTIAISILSGSIAEKMKFIPYLLFVVIWTALVYSPVAHWVWGGGWINKLGVLDFA
                     GGTVVHITSGVSGLVLAIMIGKGNKHSESTPHNLIITLIGGIFVWIGWYGFNVGSAFT
                     FDKIAMLAFTNTVISASAGSIGWLILEYIFKKTTSLLGLLLGALAGLVVITPAAGYVT
                     YLSATIMALIGGICCYIVINYIKVKLKYHDALDAFGIHGVGGIIGAALTAVFQSKKAN
                     PDIENGFIYTGDIHIILVQILCVTAVVIFSIVMTFIIAKVIKLITPLSVTEQETNIGL
                     DKIVHGEHAYFEGELNRFNKHIRY"
     gene            4073..5023
                     /locus_tag="POB55_001793"
     CDS             4073..5023
                     /locus_tag="POB55_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDI6500754.1"
                     /translation="MKNISDIAKLAGVSKSTVSRFLNNGSVSKKTSEKLTRIIAEHDY
                     QPNQFAQSLRARQTHLIGAIIPRMNSYAVDETIKGLAKQCQKYESQLILNYTGLNIEA
                     EIQALETLARSKVDGIVLMATDITERHIEVINKMNVPIVIVGQQHEQLHSIVHDDYKA
                     GQIIGEWIGQQGYQQVEVFSVSEKDIAVGIHRKRGLLDQLAKYQIKPNIHETNFTYVE
                     AQKDVANALENVEQVDAVVGATDTIALAAYKYYSDKKDVMKPHQIYGFGGDPMTQLVS
                     PSIKTIHYNYFEAGQCAMEEIQQMLKKQDMPYSVTVDVNI"
     gene            5172..6656
                     /locus_tag="POB55_001794"
     CDS             5172..6656
                     /locus_tag="POB55_001794"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HDI6500755.1"
                     /translation="MTEWTREERYQRIEDVDTEYFKTLKQQVDQSKFRQQFHIQPETG
                     LLNDPNGLIFYKGKYYVSHQWFPLGAVHGLKYWYNYTSDDLINFKAEGPILNPDTKYD
                     SHGVYSGSAFEYNGHLYYMYTGNHRDNHWQRHASQMIARLKEDGSVEKFPKPVISQQP
                     EGYTSHFRDPKVFKYSEKYYAIIGAQNNDQQGRLLLYNTEDIINWHYLGEINTELDDF
                     GYMWECPDYFNVDNQDVILICPQGIEPKGDQFKNIYQSGYILGKFDIEKLTYEHENFV
                     ELDNGFDFYAPQTFLDEKGRRVLIGWMGLPEIEYPTDNEGWAHCLTIPRVLNVENGQL
                     KQRPYPALEKLRHNKETALGYANKFTRKLHPYEGKQYELIIDILDNDATEVYFELRTS
                     KTSSTLIAYNKRENKITLDRSDSGLLPTNVEGTTRSTILDTPLKQLQIFVDTSSIEIF
                     CNDGERVLTSRIFPTEDALGIKTSTESGQVYLQFTKYDLKDEHK"
     gene            6653..7612
                     /locus_tag="POB55_001795"
     CDS             6653..7612
                     /locus_tag="POB55_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HDI6500756.1"
                     /translation="MRRLFSIGEALIDFIPNITNANLKDVQTFTKQIGGAPCNVACTV
                     QKLGQQAYMITQLGNDAFGDSIIETISSIGVDVSKVYRTNEANTALAFVSLTEAGERD
                     FSFYRKPSADMLFEPSFVNDIDVNENDVVHFCSVDLVDSPMRDAHYQLITKTLNANGT
                     VVFDPNVRLPLWDNAEDLRQTIHTFLPLAHIVKVSDEELEFITGIHDENEAIQSLFTG
                     NVTVVIYTKGADGAAVYLKNGINHYHSGYKVKPVDTTGAGDAFIGAVISRILATDVLN
                     LTQLFENEGEEILAFSNRVAAIVTTKYGAINSLPTLQEVEQAF"
     gene            complement(7800..7909)
                     /locus_tag="POB55_001796"
                     /pseudo
     CDS             complement(7800..7909)
                     /locus_tag="POB55_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500747.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7981..8697)
                     /locus_tag="POB55_001797"
     CDS             complement(7981..8697)
                     /locus_tag="POB55_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDI6500757.1"
                     /translation="MKIFICEDDPKQRENMVTIIKNYIMIEEKPMEIALATDNPYEVL
                     EQAKNMNDIGCYFLDIQLSTDINGIKLGSEIRKHDPVGNIIFVTSHSELTYLTFVYKV
                     AAMDFIFKDDPAELRTRIIDCLETAHTRLQLLSKDNSVETIELKRGSNSVYVQYDDIM
                     FFESSTKSHRLIAHLDNRQIEFYGNLKELSQLDDRFFRCHNSFVVNRHNIESIDSKER
                     IVYFKNKEHCYASVRNVKKI"
     gene            complement(8716..>9399)
                     /locus_tag="POB55_001798"
     CDS             complement(8716..>9399)
                     /locus_tag="POB55_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDI6500758.1"
                     /translation="TLKEMKYKRNQEEIETYYEYTLKIEAINNEMRKFRHDYVNILTT
                     LSEYIREDDMTGLRDYFNKNIVPMKDNLQMNALKLNGIENLKVREIKGLLTAKILRAQ
                     EMNIPISIEIPDEVTRINLNMIDLNRSIGIILDNAIEASSEIDDPIIRVAFIESENSV
                     TFIVMNKCADDIPRIHELFQESFSTKGEGRGLGLSTLKEIADNADNVLLDTIIENGFF
                     IQKVEIINN"
BASE COUNT         3250 a         1302 c         1744 g         3103 t
ORIGIN      
        1 ttcaataatg tataatattt aggtattcat tgtaatcaat tatttgtttt ttaataatct
       61 aacaattacg ctctaactcg tttgaataca tagatataat tcattcaaac acgaaggagg
      121 aaatagcgaa tgagtgacca agttaaaatt cctcgagcaa ctttaaaacg tttgccgtta
      181 tattatagat ttgtcagttc attaaaatct aaaggtatag atcgtgtaaa ttcaaaagcg
      241 attagcgatg cgttacaaat tgactcggca acaattcgtc gtgacttttc atattttggc
      301 gaattaggta aaaaagggta cggatataat atagatagtt tattggattt ctttaaatct
      361 gaactaagcg agagtgacat gatcaaaatc gcaattgtcg gagttgggaa cctagggaaa
      421 gctttgctca catataactt ttcaatacat gacgatatga cgattacaga agcgtttgac
      481 gtaaaagaag atgttattgg ccagaaaata gggaacgtta ttgttaaaga taacgatgaa
      541 ttaataacaa cattgaagaa ggaagaaata gatgttgtga ttctaactac accagaaaga
      601 gttgcacaga aagttgcaga tgaactcgtc caagctggtg tgaaaggtat tttaaacttc
      661 actcctggta gaattaatac gccttcagat gtgcaagtac atcaaattga cttaggtata
      721 gaattacagt cattattatt ctttatgaaa aattacagtg aataaatgtg atgttagctt
      781 tgaatgatta accttgttat atctttaatt aattatagaa cagtctggga ctaagtatgt
      841 ctcaggcttt ttctgttatg tttgaaaatg aaaggaaaaa ctgttgtgct gataaatata
      901 gtataggtta ataaattaac acgtagagat ataacgatga atcgtaatta atcgccttcc
      961 ttatatgaat ataatataaa tatcaaaaat attgtgtata aggttgaaaa caagaaaatc
     1021 atcacctata atacatacta aacttatcgg aaatgagagg gtaggattat gtggatgatt
     1081 tttagtggct taattgtcgg gggactctta ggttttgtca tgcaaagaac aagattttgt
     1141 ttaacaggtg gctttcgaga tatgtatgtg caaaagaata ataagatgtt ctatgcatta
     1201 ttaatcgcta ttactattca aagtatagga ttattgattt tgacggcaac agatatttta
     1261 aaaattcctg cacatagttt tccaatattg ggaacaatta taggttcttt tatttttgga
     1321 attggaatag tattggctgg agggtgtgca acaggtactt ggtatcgcgc tggtgaaggg
     1381 ctaattggta gttggattgc attagtatta tatgctgtta ctgcagcaat cactaaaaca
     1441 gggattttaa agccagtaat ggataaaatt aatcaaccaa cgaatgtaaa tagtgatatg
     1501 tctcaaacaa ctggcattcc gttttgggga ttagtcgtta tattaactat aatcaccatt
     1561 tttctagttg taagaacact caataataag aaagttcgag ttgctgttcc aaaactaaag
     1621 caacgttata caggtattag atattacctt ttcgaaaaac gataccatcc atttattgca
     1681 gcaattgtaa ttggacttat cgcactctta gcttggccaa tgagtgcatc aactggaaga
     1741 aatgacggtt taggtataac aacgccatca gcaaatttag tacacttttt gattacaggt
     1801 gaaactaaat ttattgattg gggtgtcttt ttagttctag gaattttcat tggttcatat
     1861 attgcagcta gaggatcaag agaatttaaa tggcgattgc cagataagat tacaatacga
     1921 aacagtgcta ttggtggcat atgtatggga tttggtgcgt cagttgctgg tggttgttct
     1981 atcggtaacg gtttggttga aacagcaacg atgacttggc aaggatggat tgcgctagca
     2041 tcaatgatag ttggtgtatg gacaatgagt cattttatct ttgttcgtcc aatgaaaaaa
     2101 gtacaacaac aatctgcaaa ggttaaacag caaacgcaaa tagtatagaa gattattatg
     2161 caaatgatgt tgatcaaata aaagtgattg gaaaaggaga aataattatg atacacgaat
     2221 taggtacagt aggaatggta tgtccatttc cgttaattga agcgcaaaag aaaatggcaa
     2281 cattgcaatc tggagatgaa ttaaaaattg attttgattg cacgcaagcg acggaagcta
     2341 ttccaaattg ggcagcagaa aatggatatc ctgtaacaaa ctatgaacaa attgataatg
     2401 cttcatggac aattacaatt caaaaagttt aacgttatca ttttaacaat aaaatagatg
     2461 ttagattcta tggctacttc cgctaattta aaagtgagta agtagtcttt tttgttttag
     2521 ttcatgaaat cacttttata tagtgtggca cattttatcc caaaagatgt aataaaactt
     2581 aacgcatttt tgctttttat aaattgtcag attattatga aaacaaggga gtggtaagta
     2641 tgaatcttaa cgatacgata tttatgtttt tgtgtacatt attagtttgg ctaatgacac
     2701 caggattaag tttattttat ggtgggttag ttcaatctaa aaatgcgctt aatactgtca
     2761 tgcaaagtat ggcagcaatt gtgcttgtta catttgtatg gataacagtt ggttttacaa
     2821 ttagttttgg gaatgggaat ttatggttcg gaaattggga atatactttt cttaatcatg
     2881 taggttttgc gactcaagaa gatattagcc cacatattcc tttcgctttg tttatgttat
     2941 ttcaaatgat gttttgtacg attgcaattt ctattttatc tggttcaatc gctgagaaaa
     3001 tgaagtttat tccttattta ttattcgtag taatatggac tgctcttgta tacagtccag
     3061 tagcacattg ggtttggggc ggcggttgga ttaacaaact cggtgtatta gatttcgctg
     3121 gaggtacggt tgttcatatt acatcaggtg tttctggttt agtattagct attatgattg
     3181 gaaaaggaaa caaacattct gaatcaacac cacataatct tatcattacg ttgattggcg
     3241 gtatattcgt gtggattggt tggtatggat ttaatgtagg tagtgctttt acatttgata
     3301 aaattgcgat gcttgcattt acaaatactg tcatttcagc cagtgcaggt tctataggtt
     3361 ggttaatttt agaatatatt tttaaaaaga cgacaagttt acttggactt ttactcggtg
     3421 cattagcagg attagttgtc attactcctg cagcaggata tgtaacatat cttagtgcaa
     3481 caataatggc tttaatagga ggtatctgtt gttatattgt cattaattac atcaaggtaa
     3541 aactaaaata tcatgatgca ttagatgcat ttggtatcca cggtgttggt ggtattattg
     3601 gtgctgcttt aacagcagtt ttccaaagta aaaaagcaaa tcctgacatt gaaaatggtt
     3661 ttatttatac tggtgacata catattatac ttgtacaaat attatgtgta acagcagttg
     3721 taatttttag tatcgtcatg acgtttatta ttgcgaaagt aattaaatta attacaccat
     3781 tatctgttac ggaacaagaa acgaatatag gattagacaa gattgttcac ggtgaacatg
     3841 cttactttga aggtgagcta aatagattca ataaacatat tcgatattag aatatattta
     3901 catagaatat tcattgtcct gacatttaac taaaggttga tgttgggaca ttttgttata
     3961 caaaagtttt attttgaaat ctttttatga aagaagcaga aatattattt aaagcggtta
     4021 cacatatgct aaaataaggc taagtgtcac aaataatgat aggtgaataa gtatgaaaaa
     4081 tatatctgat attgccaaat tggcaggcgt ttcaaaaagt acagtatcta gatttttaaa
     4141 taatggatct gtcagtaaaa aaacaagtga aaaattaaca agaattatag cagaacatga
     4201 ctatcaaccg aatcaatttg ctcaaagttt aagagcgaga caaacacacc ttattggtgc
     4261 cattatacct agaatgaatt catatgcagt agacgaaaca atcaaaggat tggcaaaaca
     4321 atgccaaaaa tatgaatcac aattaatttt aaattataca ggtttaaata tcgaagcaga
     4381 aatacaagcg cttgaaacat tagcacgcag taaagtagat ggtattgttt taatggctac
     4441 agatataaca gagagacata ttgaagtcat taataaaatg aatgtaccaa tcgttattgt
     4501 tggtcaacaa catgaacaac ttcatagtat tgtgcatgat gattataaag caggtcaaat
     4561 tataggcgaa tggattggtc aacagggata tcaacaagtt gaagtgttta gcgtaagtga
     4621 aaaagatatt gcagttggta tacatagaaa acgtggttta cttgaccagt tagctaaata
     4681 ccaaattaaa cctaatatac atgaaacaaa ttttacttat gtggaagcac aaaaagatgt
     4741 tgcaaatgct ttggaaaatg tggagcaagt agatgcggtt gttggagcaa ctgatacgat
     4801 tgcattagct gcctataaat attattctga taaaaaagat gttatgaaac cacatcaaat
     4861 atatggtttt ggtggtgacc caatgacaca attagtgtct ccatcgataa aaacaattca
     4921 ttataattat tttgaagctg gccaatgcgc gatggaagag atacaacaga tgcttaaaaa
     4981 gcaagatatg ccatatagcg tcacagtaga tgttaatatt tagacgctgt attttttaaa
     5041 ataaatgtgg aaccgatacc atataactat aaatggatag gttaaaagtt aaagaacgta
     5101 ggtaaaattt gctataatag aatataaatt gttaacagca taaattataa aaggaggact
     5161 gggtaaatat tatgaccgaa tggactagag aagaacgtta tcaacgaatc gaggacgttg
     5221 atactgagta ttttaaaaca ttaaaacaac aagttgatca atcaaaattt cgtcaacaat
     5281 ttcatataca accagaaaca ggcttattaa atgaccccaa cggactaatt ttttataaag
     5341 ggaagtatta tgtttcacat caatggttcc cattaggcgc agtacatggc ttaaagtatt
     5401 ggtataacta cacgagtgat gacttaataa actttaaagc tgaagggcca attttaaatc
     5461 cagatactaa atatgacagc catggtgtat atagcggtag cgcttttgaa tataacgggc
     5521 atttatatta tatgtacaca ggaaatcatc gagataatca ttggcaacga catgcgagtc
     5581 agatgatcgc acgattgaaa gaagacggtt cagttgaaaa gtttccaaag ccagtaatta
     5641 gccaacaacc agaaggatat acgagtcatt ttagagatcc taaagttttt aaatatagtg
     5701 agaaatatta tgcgatcatt ggcgcacaaa ataatgatca gcaaggtcga ttattacttt
     5761 ataatactga agatataatt aattggcatt atttaggtga aataaataca gagttggatg
     5821 attttggata tatgtgggaa tgcccagatt actttaatgt agataatcaa gatgtcatac
     5881 ttatttgtcc acaaggtatt gaaccaaaag gcgatcagtt caaaaatatt tatcaaagtg
     5941 gttatatact tggaaagttt gatattgaaa agttaacata tgaacatgaa aattttgtcg
     6001 agcttgataa tggctttgat ttctatgcac ctcaaacatt tttagatgaa aaaggccgac
     6061 gagtactaat tggatggatg gggttaccgg aaatcgaata tcctactgat aatgaaggat
     6121 gggcccattg cttaacaatt cctcgtgtat taaatgtaga aaatggtcaa cttaagcagc
     6181 gtccatatcc agcgttggaa aaattacgtc acaataaaga gacagcatta ggctacgcaa
     6241 ataaatttac tcgaaaatta catccgtatg aaggtaaaca gtatgaatta atcatagata
     6301 ttttggataa tgatgctacc gaagtgtact ttgaattacg tacatctaag acttcttcaa
     6361 cattaatcgc ttataacaag cgtgaaaata aaataacatt agatcgcagc gacagtggtt
     6421 tattgccgac aaatgttgaa ggtacgacgc gtagtacgat attagacacg ccattaaagc
     6481 aattacaaat ttttgttgat acatctagta tagaaatttt ctgtaatgat ggggaacgtg
     6541 ttttgacatc tcgaattttc ccgacagaag acgcactagg tattaaaaca tcaacagaat
     6601 cagggcaagt atatttacaa tttactaaat atgatttaaa ggatgaacat aaatgagacg
     6661 tctattttca ataggagaag cgttaattga ttttattcca aatattacaa atgcgaattt
     6721 aaaagatgtt caaacattta cgaagcaaat tggtggcgcg ccatgcaatg tagcttgtac
     6781 agttcaaaag ttaggacaac aagcgtatat gattacacaa ttaggtaatg atgcatttgg
     6841 agatagcatt attgaaacga tatcaagtat tggtgtagat gtatcaaaag tgtatcgcac
     6901 gaatgaagca aacactgcgt tagcttttgt gagtttgact gaagcgggcg agcgagattt
     6961 ttctttttat cgtaagccct ctgctgatat gttatttgaa ccaagttttg tgaatgatat
     7021 tgatgtgaat gaaaatgatg tagtacattt ttgttctgtt gatttggtcg atagtccgat
     7081 gagagatgca cattatcaat tgattaccaa aacattaaat gcaaatggta ctgtcgtatt
     7141 tgatcctaat gtaagattgc cattatggga taacgctgaa gatttaagac aaactattca
     7201 tacctttttg ccattggcac atattgtaaa agtttcagat gaagaattag aatttattac
     7261 aggtattcat gatgaaaatg aagccattca atcattattt acaggtaatg tcactgtagt
     7321 catttatacg aagggagcag atggtgctgc cgtatatttg aagaatggta ttaatcatta
     7381 tcatagtggc tataaggtaa aacctgttga tacaactggg gcaggggatg cttttattgg
     7441 tgcagttatt agcaggattt tagcaaccga tgtgttaaat ttaactcagt tatttgaaaa
     7501 tgaaggtgag gaaattttag cgtttagcaa tcgcgttgct gcaatagtga caacgaagta
     7561 tggtgctatt aatagtttgc caacattaca agaggttgaa caagcatttt aaataattca
     7621 tttataaaac agtgtaggaa acaagttgaa tcttgtgcta cactgttttt taaaacaaaa
     7681 taagattcac ggggtaggaa attgtagcat tttatttatg gaaactgtca atttcaaatt
     7741 tagtatattt tgttaaaaat tgcgccatag gattgtagag tgaaatagaa attgataaat
     7801 tatgtagtaa taggaatagg tatacaaaga aacgttacat cattttttaa ttcacgcgtc
     7861 atatttaatt ttgttattaa acaattaaaa agccagctat acagtgcatt tgctagttat
     7921 cttgttaaaa tccaacaaga tttacaattg aatacgccgt taactgactt tattatctta
     7981 ttatattttt ttaacgtttc tcaccgatgc atagcagtgt tctttatttt taaaatagac
     8041 aattcgctct ttcgaatcta tagattcaat attatggcga ttgacgacaa agctattatg
     8101 acatctaaag aaacgatcat ctaattgact cagttctttt aaattaccat aaaattcaat
     8161 ttgacggtta tctaaatggg caatgagtct gtgagatttt gttgatgatt caaaaaacat
     8221 aatatcatca tattgaacat acactgaatt actgccacgt tttaattcaa tcgtttcaac
     8281 gctattatct ttagacaaca attgtaagcg tgtatgtgca gtttctaaac aatctataat
     8341 tcgagttctt aattctgctg gatcatcttt aaaaataaaa tccatcgctg caactttgta
     8401 gacaaatgtt aaataggtaa gttcactgtg actcgtaacg aaaataatgt taccaactgg
     8461 gtcatgctta cgaatttcac tgcctaattt gataccatta atatcagttg aaagttgaat
     8521 atctaaaaag taacagccta tgtcattcat atttttagct tgctcaagca cctcataagg
     8581 attatcagtt gcgagggcaa tttccatagg cttttcttct atcattatat aatttttaat
     8641 aatggtaacc atgttttctc tttgttttgg atcgtcttcg caaatgaaaa ttttcataca
     8701 ttcacatcct tatggctagt tgttaataat ttcaactttt tgaataaaga aaccattttc
     8761 gataattgta tctaataaga cattgtctgc attatcagca atttctttta aagttgatag
     8821 acctaaacca cgaccttcac ctttagtaga aaaactttct tggaacaatt catgaatacg
     8881 tggtatatca tctgcacatt tattcataac aataaacgtt actgaatttt cgctttcaat
     8941 aaatgcaact cgaatgatag gatcatcaat ttcacttgat gcctctattg cgttatcaag
     9001 aataatacca atactgcgat ttaaatcgat catattcaaa ttaatgcgag ttacttcatc
     9061 tggtatttct atactaatcg gtatattcat ttcttgtgca cgtaaaattt tagctgttag
     9121 aagaccttta atctcacgta ctttaagatt ctcaatacca tttaacttaa gagcattcat
     9181 ttgtaaatta tctttcatag gtacaatatt tttattgaaa tagtctcgaa gaccggtcat
     9241 gtcatcttca cgaatgtatt ctgaaagagt cgtcaagata ttgacataat catgacggaa
     9301 cttgcgcatt tcattattaa ttgcttcaat ctttaatgtg tattcataat aggtttcaat
     9361 ttcttcttga ttacgtttat atttcatttc tttaagagt
//
LOCUS       DAMZKC010000101         9326 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.102, whole genome shotgun sequence.
ACCESSION   DAMZKC010000101 DAMZKC010000000
VERSION     DAMZKC010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9326
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.102"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(163..300)
                     /locus_tag="POB55_001799"
     CDS             complement(163..300)
                     /locus_tag="POB55_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500759.1"
                     /translation="MKRPEKIQNVVKLLSSLGVNIKKTKSRLDIINTLPASNKVSHEL
                     K"
     gene            complement(428..637)
                     /locus_tag="POB55_001800"
     CDS             complement(428..637)
                     /locus_tag="POB55_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HDI6500760.1"
                     /translation="MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVT
                     VSYETPANLNSIEKEIYDEGYKIVI"
     gene            complement(649..942)
                     /gene="csoR"
                     /locus_tag="POB55_001801"
     CDS             complement(649..942)
                     /gene="csoR"
                     /locus_tag="POB55_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-sensing transcriptional repressor CsoR"
                     /protein_id="HDI6500761.1"
                     /translation="MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDV
                     LTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQEEAMEELLVTFQKLIKD"
     gene            1108..2592
                     /gene="cls"
                     /locus_tag="POB55_001802"
     CDS             1108..2592
                     /gene="cls"
                     /locus_tag="POB55_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDI6500762.1"
                     /translation="MIELLSIALKHSNIILNSIFIGAFILNLLFAFTIIFMERRSANS
                     IWAWLLVLVFLPLFGFILYLLLGRQIQRDQIFKIDKEDKKGLELIVDEQLAALKNENF
                     SNSNYQIVKFKEMIQMLLYNNAAFLTTDNDLKIYTDGQEKFDDLIQDIRNATDYIHFQ
                     YYIIQNDELGRTILNELGKKAEQGVEVKILYDDMGSRGLRKKGLRPFRNKGGHAEAFF
                     PSKLPLINLRMNNRNHRKIVVIDGQIGYVGGFNVGDEYLGKSKKFGYWRDTHLRIVGD
                     AVNALQLRFILDWNSQATRDHISYDDRYFPDVNSGGTIGVQIASSGPDEEWEQIKYGY
                     LKMISSAKKSIYIQSPYFIPDQAFLDSIKIAALGGVDVNIMIPNKPDHPFVFWATLKN
                     AASLLDAGVKVFHYDNGFLHSKTLVIDDEIASVGTANMDHRSFTLNFEVNAFIYDQQI
                     AKKLKQAFIDDLAVSSELTKARYAKRSLWIKFKEGISQLLSPIL"
     gene            2620..3267
                     /locus_tag="POB55_001803"
     CDS             2620..3267
                     /locus_tag="POB55_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDI6500763.1"
                     /translation="MQQSDVISAAKKYMESIHQNDYTGHDIAHVYRVTALAKSIAENE
                     GVNDTLVIELACLLHDTVDEKVVDANKLYVELKSFLSSLSLSTEDQEHILFIINNMSY
                     RNGKNDHVTLSLEGQIVRDADRLDAIDAIGVARTFQFAGHFGEPMWTEHMSLDKINDD
                     LVEQLPPSAIKHFFEKLLKLESLMHTDTAKMIAKERHDFMMMYLKQFFTEWNYHD"
     gene            complement(3881..4753)
                     /gene="yidC"
                     /locus_tag="POB55_001804"
     CDS             complement(3881..4753)
                     /gene="yidC"
                     /locus_tag="POB55_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDI6500764.1"
                     /translation="MKKKALLPLFLGIMVFLAGCDYSKPEKRSGFFYNTFVDPMKNVL
                     DWLGNNLLNDNYGLAIIILVLVIRIILLPFMLSNYKNSHMMRQKMKVAKPEVEKIQEK
                     VKRARTQEEKMAANQELMQVYKKYDMNPIKSMLGCLPMLIQLPIIMGLYFVLKDQLVD
                     GLFKYPHFLWFDLGRPDIWITIIAGVLYFIQAYVSSKTMPDEQRQMGYMMMVISPIMI
                     IWISLSSASALGLYWSVSAAFLVVQTHFANIYYEKVAKKEVQPFIEAYEREHNGGSNK
                     KGKNTQVVSKKKKK"
     gene            complement(4839..5480)
                     /locus_tag="POB55_001805"
     CDS             complement(4839..5480)
                     /locus_tag="POB55_001805"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HDI6500765.1"
                     /translation="MFNQSYLNVYFICGTSDVPSHRTIHEVLEAALKAGITLFQFREK
                     GESALKGNDKLVLAKELQHLCHQYDVPFIVNDDVSLAKEINADGIHVGQDDAKVKEIA
                     QYFTDKIIGLSISDLDEYAKSDLTHVDYIGVGPIYPTPSKHDAHIPVGPEMIATFKEM
                     NPQLPIVAIGGINTNNVAPIVEAGANGISVISAISKSENIENTVNRFKDFFNN"
     gene            complement(5473..6273)
                     /gene="thiM"
                     /locus_tag="POB55_001806"
     CDS             complement(5473..6273)
                     /gene="thiM"
                     /locus_tag="POB55_001806"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HDI6500766.1"
                     /translation="MNYLNKIRIENPLTICYTNDVVKNFTANGLLSIGASPAMSEAPE
                     EAEEFYKVAQGLLINIGTLTAENEQDIIAIAQTANEAGLPIVFDPVAVGASTYRKQFC
                     KLLLKSAKVSVIKGNASEILALIDDTATMKGTDSDANLDAVTIAKKAYATYKTAIVIT
                     GKEDVIVQDNKAIVLANGSPLLARVTGAGCLLGGVIAGFLFRETEPDIEALIEAVSVF
                     NIAAEVAAENENCGGPGTFSPLLLDTLYHLNETTYQQRIRIQEVEKYV"
     gene            complement(6257..7087)
                     /gene="thiD"
                     /locus_tag="POB55_001807"
     CDS             complement(6257..7087)
                     /gene="thiD"
                     /locus_tag="POB55_001807"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HDI6500767.1"
                     /translation="MIKPKIALTIAGTDPTGGAGVMADLKSFHSCGVYGMGVVTSIVA
                     QNTLGVQHIHNLNHQWVDEQLDSVFNDTLPHAIKTGMIATADTMETIRHYLMQHESIP
                     YVIDPVMLAKSGDSLMDNDTKQNLQHTLLPLADVVTPNLPEAEEITGLTIDSEEKIMQ
                     AGRIFINEIGSKGVIIKGGHSNDTDIAKDYLFTNEGVQTFENERFKTKHTHGTGCTFS
                     AVITAELAKGRRLFEAVHKAKKFISMSIQYTPEIGRGRGPVNHFAYLKKEGLDDELSK
                     "
     gene            complement(7080..7769)
                     /gene="tenA"
                     /locus_tag="POB55_001808"
     CDS             complement(7080..7769)
                     /gene="tenA"
                     /locus_tag="POB55_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HDI6500768.1"
                     /translation="MEFSQKLYQAAKPIINDIYEDDFIQKMLSGDIGADALRHYLKAD
                     AAYLKEFTNLYALLIPKMNSMNDVKFLVEQIEFMVEGEVLAHDILAQIVGESYEEIIK
                     TKVWPPSGDHYIKHMYFQAHSRENAIYTIAAMAPCPYIYAELAKRSQSDHKLNREKDT
                     AKWFDFYSTEMDDIINVFEALMNKLAESMSDKELEQVKQVFLESCIHERRFFNMAMTL
                     EQWEFGGKVND"
     regulatory      complement(7849..7951)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(8293..8988)
                     /gene="sceD"
                     /locus_tag="POB55_001809"
     CDS             complement(8293..8988)
                     /gene="sceD"
                     /locus_tag="POB55_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase SceD"
                     /protein_id="HDI6500769.1"
                     /translation="MKKTLLASSLAVGLGIVAGNAGHEAHASEADLNKASLAQMAQSN
                     DQTLNQKPIEAGAYNYTFDYEGFTYHFESDGTHFAWNYHATGTNGADMSAQAPATNNV
                     APSAVQANQVQSQEVEAPQNAQTQQPQASTSNNSQVTATPTESKSSEGSSVNVNAHLK
                     QIAQRESGGNIHAVNPTSGAAGKYQFLQSTWDSVAPAKYKGVSPANAPESVQDAAAVK
                     LYNTGGAGHWVTA"
BASE COUNT         2867 a         1695 c         1481 g         3283 t
ORIGIN      
        1 ataataacaa tccctctttg aggtacacat gtaaaatgta tactctaaag agggatttag
       61 ttatatacta cttgctttca ttttagtaaa tccagtcaaa aaagctatca tcgaatagtc
      121 caacgatagc tttaacggtg tgtgattcac aatcacagtc aattatttta attcgtgact
      181 tactttatta gatgctggca aagtattaat aatgtctaaa cgagatttag tttttttaat
      241 attcacacct aatgatgaca atagtttgac tacattttga atcttttcag gacgtttcat
      301 tattatcacc tcgtttggat cgctttcaat attatcttac cctattcgac atacctttac
      361 tacatcattt acaaaatttt taatttattt tcaaaattaa aatctatagc gcactctaca
      421 ttatacccta aattacaatt ttgtatcctt catcatagat ttctttttca atactattca
      481 aatttgctgg tgtctcatac gatacagtta cttgaccatc tattatatta atattcactt
      541 gatgaacacc tatcatattt gaaatgcgtt ctgtcaattg acttacttgt tcttctgttt
      601 caattcccgc tgtgtaaatc gtattttgat gtatcatctt aaactccttt agtctttaat
      661 caatttttga aaagtcacta ataactcttc catcgcctct tcctgagcac cttgattaac
      721 tttattcata atacaacttt tcatatgttg ttctaataac tttatcgcaa cactgtttaa
      781 cgccgaacgt gtcgctctta tttgcgtaag gacatcatca caatagacat cttcttcaat
      841 catgcgatta atcgctctca cttgtccttc aattcgattt aaacgtgatt taagattcgt
      901 ttttatttgt tcagaatgat gtgcattatc ttgttcagtc atagcattca ctccttcatt
      961 tacattcttt actatactat atcccataaa acacaacgtc aacacaaagt gtgtttttca
     1021 aactaaattg tcatgatatt taaattgctt catgatacaa ttaataagat atactactcg
     1081 tttaagattt tcgatgggag gtttcaaatg atagagttat tatccattgc actcaaacat
     1141 tctaatatta ttttaaattc aatatttatt ggtgcattta ttttaaactt attattcgcc
     1201 tttaccatta ttttcatgga aagacgttct gccaattcta tctgggcttg gttactagtc
     1261 ttagttttct tgcctttatt cggcttcatt ttatacttac tattaggacg acaaattcaa
     1321 cgtgaccaaa ttttcaaaat tgataaggaa gataaaaaag gattagagtt aatcgttgat
     1381 gagcaattag ctgctttaaa aaatgaaaac ttttcaaatt ccaattatca aattgtaaaa
     1441 tttaaagaaa tgattcaaat gttgttatat aataacgcag catttttaac aacagacaat
     1501 gatttaaaaa tatacacaga cggccaagaa aaatttgatg acctaataca agacatccgt
     1561 aatgctactg attatattca ttttcagtac tatattattc aaaatgatga attaggtcgt
     1621 accattttaa atgaacttgg taaaaaagcg gaacaaggtg tagaagttaa aattctttat
     1681 gatgacatgg gttctcgtgg actgcgtaaa aaaggcttac gcccgtttcg caataaaggt
     1741 ggacatgctg aagcattttt cccatcaaaa ttacctttaa ttaacttgcg tatgaacaat
     1801 cgaaaccatc gaaaaattgt tgtaatagat gggcaaattg gatatgttgg tggttttaat
     1861 gttggtgatg agtacttagg taaatcaaaa aaattcggct attggcgaga tacgcattta
     1921 cgaattgtcg gggacgcagt gaatgcattg caattacgat ttattctaga ttggaattca
     1981 caagccacac gtgaccacat ctcctatgat gatcgttatt tcccagatgt aaattctggt
     2041 ggaacaattg gcgttcaaat agcttctagt ggtcctgacg aagaatggga acagattaaa
     2101 tacggttatt tgaaaatgat ttcatctgct aaaaaatcga tttatattca atctccctat
     2161 tttatacctg atcaagcctt tttagattct attaaaattg cggcattagg tggtgttgat
     2221 gtcaatatca tgattcctaa taaacctgac catccgtttg ttttttgggc tactttaaaa
     2281 aatgcagcat ccttattaga tgccggtgtt aaagtatttc actacgacaa tggcttttta
     2341 cactcaaaaa cacttgttat agatgatgaa attgcaagtg ttggaacagc taatatggac
     2401 catcgcagtt tcacattgaa tttcgaagtc aacgccttta tttatgacca acaaattgcc
     2461 aaaaaattaa aacaagcttt tatagatgat ttagcagtat cttctgaatt aacaaaagca
     2521 cgttatgcta agcgaagtct ttggattaaa tttaaagaag gtatttcaca attattgtca
     2581 cctatcttat aaaatagaaa tatgaggagt gtaactttaa tgcaacaatc agacgtcatt
     2641 agtgctgcca aaaaatatat ggaatctatt catcaaaatg attatacagg ccatgatatt
     2701 gcgcatgtat atcgtgtcac tgctttagct aaatcaatcg ctgaaaatga aggtgttaat
     2761 gatactttag tcattgaact cgcatgtttg cttcatgata ccgttgacga aaaagttgta
     2821 gatgctaaca aactatatgt tgaattgaag tcatttttat cttctttatc actatcaacc
     2881 gaagatcaag agcacatttt atttattatt aataatatga gctatcgcaa tggcaaaaat
     2941 gatcatgtca ctttatcttt agaaggtcaa attgtcaggg atgcagatcg tcttgatgct
     3001 atagacgcta taggtgttgc acgaacattt caatttgcag gacactttgg tgaaccaatg
     3061 tggacagaac atatgtcact agataagatt aatgatgatt tagttgaaca gttgccacca
     3121 tctgcaatta aacatttctt tgaaaaatta cttaagttag aatctttaat gcatacagat
     3181 acggcgaaga tgattgctaa agaacgtcac gactttatga tgatgtactt gaaacagttt
     3241 tttacggaat ggaattatca cgactagaca ttgaagttgt agtatgatga tgcgatgtaa
     3301 tggcgtgttg ttgtggaagc ttggtgtcat gccatgttac tttgatgtgt tgttgtggaa
     3361 gcttggtgtt gatgccatgt tactttgatg tgctggtgct acgatgcgac ttgatgtagt
     3421 gctatgatgt ggcattgcgg tgttatggtg ttatagaccg gtttggcgtc gatgccatgc
     3481 cgttacgatt ctatgatatg ttgttgggac gttgcaatgt gtattatgcc gttgtgacgt
     3541 tattatttca cactgttaca tgtataagtg aattgctgtg gaaatttgcg acatatactg
     3601 ctacactgat gaatcattgt gtcaagatga cattgcgatg aagaatgaca actctgttat
     3661 taaccacttt ttacatactg aaaactcgtt aatattattt caaataaaaa cagcagtagg
     3721 atgactttca catttgaaat catcttactg ctgtttctat ttatcacata ttgtataatg
     3781 tgacactaag tttcgctttt gaagcgaaaa ataatgtgcg ccctataaag ttaaaattat
     3841 attcaacttt tagggtgcac attatttgga cttgctaagg ttatttcttt ttctttttag
     3901 acactacttg tgtgtttttg ccttttttat tgctgccgcc gttgtgctct ctttcatacg
     3961 cttcaatgaa aggttgtact tcttttttag cgactttttc ataataaatg ttcgcaaagt
     4021 gtgtttgaac tacaaggaac gccgcactga ctgaccagta caaaccaagt gctgatgctg
     4081 agcttaatga aatccagata atcataattg gtgaaatgac catcatcatg taacccattt
     4141 gacgttgttc gtctggcatc gttttacttg atacatatgc ttggataaag tataaaacac
     4201 cggcaataat tgtaatccaa atatcaggac gtcctaaatc gaaccataag aagtgtggat
     4261 atttaaacaa accatctaca agttggtctt taagtacaaa gtataatccc atgatgattg
     4321 gcaattggat tagcattggt aaacaaccca acatactctt aatcgggttc atgtcatact
     4381 ttttatatac ttgcattaat tcttggtttg cagccatttt ttcttcttgt gtacgcgcac
     4441 gtttcacttt ttcttgaatt ttttcaactt ctggctttgc aactttcatt ttttgacgca
     4501 tcatatgact atttttatag tttgacaaca tgaatggtaa taaaataata cgaattacca
     4561 atacaaggat aataatagct aaaccataat tgtcgtttaa taagttattt cccaaccaat
     4621 ccaatacatt tttcattgga tctacgaatg tattgtagaa aaacccacta cgtttttcag
     4681 gtttagaata gtcacaacca gccaaaaaga ccataatacc taaaaataat ggtagtaacg
     4741 cttttttctt catttttcca cctctatcat tatattcaca taggatttat tctatcacat
     4801 taatgagtac gtatgaaaca ataagtggaa aaatttaact aattattaaa aaaatctttg
     4861 aatcgattaa cagtattttc aatattttca cttttagaaa tggctgaaat gactgaaatt
     4921 ccattggcac ctgcttctac aatcggcgcc acattattag tattgatacc gccaatagct
     4981 acaatcggta gttgcggatt catttcttta aacgttgcaa tcatttctgg acctactggt
     5041 atatgcgcgt catgcttcga cggcgtagga tagattggtc caacacctat ataatcaaca
     5101 tgagttaaat cagattttgc atactcatct aaatcactaa tactaagtcc aataatttta
     5161 tcagtgaaat attgtgctat ctctttgact ttcgcatcat cttgaccgac atgtatacca
     5221 tccgcgttaa tttcttttgc caaggataca tcatcattaa cgataaaagg cacatcatat
     5281 tgatgacaga gatgctgtaa ttctttagct aatacaagtt tatcgtttcc ttttaaagct
     5341 gattcaccct tttctcgaaa ttggaaaagt gtaatccctg cctttaatgc tgcttctaat
     5401 acttcatgaa tagttcgatg actcggaaca tcggaggtcc cacaaataaa atacacattt
     5461 agatacgatt ggttaaacat atttttccac ctcttgaata cgaatccgtt gttgataggt
     5521 tgtttcattt aaatgatata acgtatcaag caacaatggt gaaaacgtac caggaccacc
     5581 acaattttca ttttcagcag ctacctcagc agcaatatta aatacgctta ccgcttcaat
     5641 taacgcttct atgtctggtt ctgtttctct aaataaaaat ccagcaataa cgcctcctaa
     5701 taaacaacca gctccagtta ctcgtgctaa taatggagat ccattagcta atacgatggc
     5761 tttattatct tgaacaataa cgtcctcttt acctgtgatt actattgcag ttttatatgt
     5821 tgcgtaagcc tttttcgcta ttgtaaccgc atcaagatta gcatcactat ctgtaccttt
     5881 catagttgct gtatcatcaa tcaacgctaa tatttcagat gcattgcctt taattactga
     5941 tactttcgct gatttcaata ataatttaca aaattgcttt cgatatgtag aagcaccaac
     6001 agctacaggg tcaaatacaa taggtaagcc tgcctcattt gccgtttgag caatcgcaat
     6061 aatatcttgt tcattttctg ctgttaaagt accgatattg attaatagcc cttgtgcaac
     6121 tttgtaaaat tcttcagctt cttcgggagc ctcactcatt gcagggctag caccaatact
     6181 taataaacca ttcgctgtaa aatttttaac tacatcgttt gtataacaaa ttgttaatgg
     6241 gttttcaata cgtattttat ttagataatt catcgtctaa tccctctttc tttaaatatg
     6301 caaaatgatt cactggacct ctaccacggc cgatttcagg cgtatattgt atactcattg
     6361 aaataaactt tttagccttg tgtacagcct caaataatcg tctacctttt gcaagttctg
     6421 ccgttataac tgctgaaaat gtacaccctg ttccatgcgt atgttttgtt ttaaatcgtt
     6481 cattttcaaa tgtttgaaca ccttcgttag taaataaata atcttttgct atatcagtat
     6541 catttgaatg accgccttta atgatgacac ctttactacc aatctcatta ataaagatgc
     6601 ggccagcctg cataattttt tcttcactat caatggttag tcccgttatt tcttcagctt
     6661 ctggtaaatt cggtgttact acgtcagcta atggcaataa cgtatgctgc aagttttgct
     6721 ttgtgtcatt atccattaat gaatcaccgc ttttcgccaa cataacagga tcaattacat
     6781 atggaataga ttcatgttgc attaaataat gacgaatcgt ttccatagta tctgctgtag
     6841 caatcatccc cgttttaata gcatgaggta aggtatcatt gaagacacta tcaagttgtt
     6901 catctaccca ttgatgattt aaattatgaa tatgttgtac gcccaatgta ttttgagcaa
     6961 caatacttgt aacgacgccc ataccatata caccacatga atgaaatgat tttaaatcag
     7021 ccattacgcc ggcaccacct gttggatcag tacctgcaat ggttaatgct attttaggtt
     7081 taatcattta cttttcctcc aaattcccat tgttctagtg tcatagccat attgaaaaat
     7141 cgacgctcat gtatgcaact ttctaaaaat acttgcttta cttgttccaa ttccttgtca
     7201 gacattgatt cggctaattt attcattaat gcctcaaata cgttaattat gtcatccatt
     7261 tcagtactat aaaaatcaaa ccattttgcc gtatcttttt ctctattaag cttatgatca
     7321 ctttgagagc gcttagctaa ttctgcataa atatatggac aaggtgccat tgcagcaatt
     7381 gtataaatag cattttcacg actatgcgct tggaaataca tatgttttat gtaatggtcg
     7441 ccacttggag gccaaacttt tgttttaatg atttcttcgt atgattcacc aacaatttgc
     7501 gctaaaatat catgcgcaag tacttcacct tcaaccataa attctatttg ctctactaaa
     7561 aattttacgt cattcatgct attcattttt ggaattaaca aagcatataa gtttgtaaat
     7621 tcttttaaat acgcagcatc agcttttaag taatggcgta atgcgtcagc tcctatatct
     7681 ccggataaca tcttctgaat aaagtcatcc tcataaatat cattaatgat tggctttgca
     7741 gcttggtaca atttttgtga aaattccatt gtaaaaaatc ctccctaaat aaaaaaacta
     7801 cttccaacat gaaagtagtt tgatggcaat gttgctatac tagccccatc acttcaataa
     7861 ctactttcct acgttggtac taaccaaatc aggtcataag ggtctgaaca attcatctca
     7921 gccatatcat taggctcccc tagtagttcc ttagtattca attgcaaatt aatcttagca
     7981 aacggtttca acattttcaa ttattgttgc tcagttggga gtgggacaga aatgatattt
     8041 tcgcaaaatt tatttcgtcg tcccaccccg gcaaggttga ctagaattga aaaaagcttg
     8101 ttacaagcgc attttcgttc agtcaactac tgccaatata acttcgtaga gcatagaaca
     8161 ttgatttatg tcccagcctg tataatgaca tatatttgcg aaataaaaaa accggaacat
     8221 atcgagaatt ccccgatata ttccaatcta aaagttactt atataactat taattagcta
     8281 tgcataaatg gcttatgcag taacccaatg tccagcgcca ccagtgttat ataattttac
     8341 tgctgcggca tcttgaacac tttcaggagc atttgctggt gatacacctt tatatttagc
     8401 aggtgctact gaatcccaag ttgattgtaa gaattgatac ttaccagctg cacctgatgt
     8461 tggatttaca gcatgaatat tgccacctga ttcacgttga gcaatttgtt ttagatgagc
     8521 attcacattt actgatgaac cttctgatga ttttgattca gttggtgttg cagtaacttg
     8581 tgaattgttt gatgttgatg cttgtggttg ttgagtttga gcattttgtg gtgcttcaac
     8641 ttcttgtgat tgtacttgat tagcttgaac agctgatggt gcaacattat tagttgcagg
     8701 tgcttgtgca ctcatgtctg ctccattagt acctgttgca tggtaattcc aagcaaagtg
     8761 tgtaccatct gattcaaagt gataagtaaa cccttcatag tcaaatgtat aattataagc
     8821 cccagcttca attggttttt gatttaatgt ttgatcattt gattgcgcca tttgcgctaa
     8881 agatgcttta tttaagtccg cttcacttgc atgggcttcg tgacctgcat ttcctgctac
     8941 gattcctaaa cctactgcta atgatgatgc gagtaatgtt ttcttcataa tcttaaaatc
     9001 ctcctacaag taatttgttt ctctaaaagt tttacagaga ctactgtaac atctataaaa
     9061 tccgtgcagg ttacaacaaa ataaaaaaat agccttgttt tttacacagt gattacaacg
     9121 aaatccccca taatttcaga tatgattcta tcgagaattg tcatgacagt aaaaatgttt
     9181 tttatttgca attttttatg taatcgtttt gcaatctatg tctattttct attagaaaaa
     9241 ctacacaaaa agacgaccta cacaacagta ctagtcatgt aagtcgtttt taagtgagta
     9301 tatttcaagt taattagatc tataaa
//
LOCUS       DAMZKC010000102         9221 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.103, whole genome shotgun sequence.
ACCESSION   DAMZKC010000102 DAMZKC010000000
VERSION     DAMZKC010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9221
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.103"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            227..1237
                     /gene="adhP"
                     /locus_tag="POB55_001810"
     CDS             227..1237
                     /gene="adhP"
                     /locus_tag="POB55_001810"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HDI6500770.1"
                     /translation="MRAAVVTKDHKVSIEDKKLRALKPGEALVQTEYCGVCHTDLHVK
                     NADFGDVTGVTLGHEGIGKVIEVAEDVESLKIGDRVSIAWMFESCGRCEYCTTGRETL
                     CRSVKNAGYTVDGAMAEQVIVTADYAVKVPEKLDPAAASSITCAGVTTYKAVKVSNVK
                     PGQWLGVFGIGGLGNLALQYAKNVMGAKIVAFDINDDKLAFAKELGADAIINSKDVDP
                     VAEVMKLTDNKGLDATVVTSVAKTPFNQAVDVVKAGARVVAVGLPVDKMNLDIPRLVL
                     DGIEVVGSLVGTRQDLREAFEFAAENKVTPKVQLRKLEEINDIFEEMENGTITGRMVI
                     KF"
     gene            1314..1421
                     /locus_tag="POB55_001811"
     CDS             1314..1421
                     /locus_tag="POB55_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500771.1"
                     /translation="MKDSENLVFFGFEVLFVFYKMAYIYEALIKYGIVN"
     gene            1514..1930
                     /locus_tag="POB55_001812"
     CDS             1514..1930
                     /locus_tag="POB55_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HDI6500772.1"
                     /translation="MDKKVSIQTKQVLKQHNEKEKFEFTTEGTWQQRQSNFIRYVEQI
                     EDATVNVTIKVDDDSVKLIRKGDINMNLHFVEGQTTTTFYDISAGRIPLDVKTLRILH
                     FVSGDGGKLKIHYELYQDNEKMGSYQYEINYKEIGE"
     gene            1927..3588
                     /locus_tag="POB55_001813"
     CDS             1927..3588
                     /locus_tag="POB55_001813"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HDI6500773.1"
                     /translation="MNIIDQVKQTLVEEIAASINKAGLADEIPDIKIEVPKDTKNGDY
                     ATNIAMVLTKIAKRNPREIAQAIVDNLDTEKAHVKQIDIAGPGFINFYLDNQYLTAII
                     PEAIEKGDQFGHVNESKGQNVLLEYVSANPTGDLHIGHARNAAVGDALANILTAAGYN
                     VTREYYINDAGNQITNLARSIETRFFEALGDNSYSMPEDGYNGKDIIEIGKDLAEKHP
                     EIKDYSEEARLKEFRKLGVEYEMAKLKNDLAEFNTHFDNWFSETSLYEKCEILEVLAK
                     MKELGYTYEADGATWLRTTDFKDDKDRVLIKNDGTYTYFLPDIAYHFDKVKRGNDILI
                     DLFGADHHGYINRLKASLETFGVDSNRLEIQIMQMVRLMENGKEVKMSKRTGNAITLR
                     EIMDEVGVDAARYFLTMRSPDSHFDFDMELAKEQSQDNPVYYAQYAHARICSILKQAK
                     EQGIEVTAANDFTTITNEKAIELLKKVADFEPTIESAAEHRSAHRITNYIQDLASHFH
                     KFYNAEKVLTDDIEKTKAHVAMIEAVRITLKNALAMVGVSAPESM"
     gene            3979..4614
                     /locus_tag="POB55_001814"
     CDS             3979..4614
                     /locus_tag="POB55_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HDI6500774.1"
                     /translation="MLGTDELYKVLYEHLGPQFWWPADNDIEMMLGAILVQNTRWRNA
                     EIALNQVKEHTHFNPNHILELPIETLQSLIHSSGFYKSKSLTIKTLLTWLARHHFNYQ
                     EINERYKGGLRKELLSLKGIGSETADVLLVYIFGRIEFIPDSYTRKIYDKLGYENTKN
                     YDQLKKVVTLPNHFTNQDANEFHALLDVFGKHYFRDKDIKNYDFLEPYFKK"
     gene            4865..5752
                     /locus_tag="POB55_001815"
     CDS             4865..5752
                     /locus_tag="POB55_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDI6500775.1"
                     /translation="MKKSLIAFILIFMLVLSGCGMKDNDKQGSNDNGSSKSPYHRIVS
                     LMPSNTEILYELGLGKYIVGVSTVDDYPKDVKEGKKQFDALNLNKEELLKEKPDLILA
                     HESQKATANKVLSSLEKQGIKVVYVKDAQSIDETYNTFKQIGKLTHHDKQAEQLVEET
                     KDNIDKVIDSIPAHHKKSKVFIEVSSKPEIYTAGKHTFFNDMLEKLEAQNVYSDINGW
                     NPVTKESIIKKNPDILISTEAKTRSDYMDIIKKRGGFNKINAVKNTRIEVVNGDEVSR
                     PGPRIDEGLKELRDAIYRK"
     gene            5901..6851
                     /locus_tag="POB55_001816"
     CDS             5901..6851
                     /locus_tag="POB55_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDI6500776.1"
                     /translation="MTFNKVLLSWIVILIITTSIYLFWQLGDINDVFNQSILINVRLP
                     RLLEALLTGMILTVAGLIFQTVLNNALADSFTLGLASGATFGSGLALFLGLTTLWIPV
                     FSITFSLITLITVLVITSVLSQGYPVRILILSGLMIGALFNSLLYFLILLKPRKLNTI
                     ANYLFGGFGDAEYSNVSIIAITFIIALFGIFIILNQLKLLQLGELKSQSLGLNVQLIT
                     YIALCIASMITAINVAYVGIIGFIGMVIPQLIRKWQWKQSLGRQLALNIVIGGQIMVM
                     ADFIGSHILSPVQIPASIIIALIGIPVLFYMLISQSKRLH"
     gene            6906..7625
                     /locus_tag="POB55_001817"
     CDS             6906..7625
                     /locus_tag="POB55_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDI6500777.1"
                     /translation="MDLNQIKAVVFDLEGTLLDRVKSREKFIEEQYERFHDYLIHVQL
                     ADFKKAFIELDDDEDNDKPDLYKEIIKRFHVDRLTWKDLFNDFEMHFYRYVFPYYDTL
                     YTLEKLSQKGFQIGVIANGKSKIKQFRLHSLGLMHVINYLSTSETVGFRKPHPKIFED
                     MIDQLGVLPEQIMYVGDDALNDVAPARAMGMVSVWYKQEDAEIEPLEEEVDFTITTVE
                     ELLTILPIKNDNKGENYGSIY"
     gene            7609..8409
                     /locus_tag="POB55_001818"
     CDS             7609..8409
                     /locus_tag="POB55_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDI6500778.1"
                     /translation="MDLFTRKDGTSIHYSTLGEGYPIVLIHTVLDNYSVFNKLAAQLA
                     KSFQVVLIDLRGHGYSDKPRHIEIKDFSDDIVELLKYLYIEEVAFVCHEMGGIIGADI
                     SVRYPEFTSSLMLVNPTSIEGELPEERLFRKYAHIIRNWDPEKQDKFLNKRKYYRPRK
                     MNRFLKHVVDTNEISTKEEIQAVKEVFKNADISQTYRNVVVPTKIIAGEFGERTTRLE
                     AKEVADLIQNADFEVYQESSAFPFVEEQERFVEDTASFINKHHDEKHV"
     gene            8545..9051
                     /locus_tag="POB55_001819"
     CDS             8545..9051
                     /locus_tag="POB55_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500779.1"
                     /translation="MKKLLTASIIACSVVMGVGLVNTSAEAASGNSIDTVKQLIKGDQ
                     SLENVKIGESIKDVLTKYKNPMYSYNEDGTEHYYEFHTKKGMLLVTTDGKKNNGKVTH
                     ISMMYNDANGPTYQAVKNYVGKAVTHTEYSKVAGNFGYIEKGKTTYQFASAPKDKNIK
                     LYRIDLEK"
BASE COUNT         3372 a         1188 c         1768 g         2893 t
ORIGIN      
        1 aattacacgt tagcatatcg attatgtcat ttcttttaac atattaacta gggaaacgtt
       61 aaaagttaac ggttgatatc taactaaaaa caaggtcaca gtagtatgtt ttaatacggc
      121 gtctattaca aataaaaatt acatctataa ttattcgttt tcttttttga aagtaatcgc
      181 caattaatat catacatact ggagtgacta taaggaggac attattatga gagcagcagt
      241 tgtaacgaaa gatcacaaag taagtattga ggacaaaaag ttaagagctt taaaacctgg
      301 tgaagcgttg gtacaaacgg aatattgtgg cgtttgtcat accgatttac atgttaagaa
      361 tgctgatttt ggtgatgtta caggcgttac tttaggtcat gaaggtattg gtaaagtcat
      421 cgaagttgcg gaagatgtag aatcattaaa aattggagac cgtgtgtcta tcgcttggat
      481 gttcgaaagc tgtggaagat gtgaatattg tacaacaggt cgtgaaacac tttgccgtag
      541 tgtgaaaaat gctggttata cagtagatgg tgcaatggct gaacaagtta ttgttactgc
      601 agactatgct gtgaaagtac ctgaaaaatt agatccagca gcagcgtctt ctattacatg
      661 cgcaggtgtg acaacttata aagctgtaaa agtaagtaat gtaaaacctg gtcaatggtt
      721 aggtgttttt ggtataggtg gtttaggtaa cctagcttta caatatgcta aaaacgttat
      781 gggggctaaa attgttgcct tcgacattaa tgatgataaa ttagcattcg cgaaagaatt
      841 aggtgcagat gctattatta attctaaaga tgttgatcca gttgcagaag ttatgaaatt
      901 aactgataac aaaggattag atgcaacagt ggtaacttca gttgctaaga cgccatttaa
      961 ccaagcggtt gatgttgtaa aagctggtgc aagagttgtt gctgttggtt tacctgttga
     1021 taaaatgaac ttagatatcc caagattagt gcttgacggt attgaagtag taggttcact
     1081 tgttggtaca agacaagact tacgtgaagc gtttgaattt gctgctgaaa ataaagtaac
     1141 acctaaagtt caattaagaa aattagaaga aatcaatgat atttttgaag aaatggaaaa
     1201 tggtactata actggtagaa tggttattaa attttaaaaa tattaattga ttaaatatag
     1261 atgaagaagg tagtgctctg aacactatca ttataatcaa acacgaggtt ttcatgaaag
     1321 atagtgaaaa tctcgtgttt tttggttttg aggtgttgtt tgtattttat aaaatggctt
     1381 acatatatga agcgttgatt aagtatggaa ttgttaatta attgaaccta tttagcttta
     1441 agaaggcata acaagatgac cttattttat gctataatat ttctattatg cgaagattaa
     1501 ggtgagtagt aaattggata aaaaagtaag tattcaaaca aagcaagtgt tgaaacagca
     1561 caacgaaaaa gaaaaatttg aatttactac tgaaggaact tggcaacaaa ggcaatctaa
     1621 ctttattcgg tatgtagaac aaattgagga tgcaacagtt aatgttacaa taaaagtgga
     1681 tgatgatagc gttaagttga ttcgtaaagg cgacattaat atgaatttgc attttgttga
     1741 aggacaaacg acaacaactt tttacgatat atcggctgga cgaattccac tagatgttaa
     1801 aacattacgc attttacatt tcgtaagtgg agacggtggc aagctaaaga ttcattatga
     1861 attatatcaa gataatgaaa aaatgggttc ttatcaatat gaaattaact ataaggagat
     1921 aggcgaatga atattattga tcaagtgaaa caaacattag tagaagaaat tgcagcaagt
     1981 attaacaaag caggattagc agatgagatt cctgatatta aaattgaagt tcctaaagat
     2041 acaaaaaatg gagattatgc tactaatatt gcgatggtac tgactaagat tgcaaagcgt
     2101 aatcctcgtg aaattgctca agcgattgtt gataacttag atactgaaaa agcacatgta
     2161 aaacaaattg acattgctgg tccaggattc attaattttt acttagataa tcagtattta
     2221 acagcaatta ttcctgaagc aattgaaaaa ggtgatcaat ttggacatgt aaatgaatca
     2281 aaaggtcaaa atgtattgct tgagtatgtt tcggctaacc ctacaggaga tttacatatt
     2341 ggtcatgcta gaaatgcagc agttggtgat gctttagcta atattttaac tgcagctggc
     2401 tataatgtaa cacgtgaata ttatattaat gatgctggta atcaaattac taacttagcg
     2461 cgttcgattg aaacacgttt ctttgaagct ttaggtgaca atagttattc aatgccagaa
     2521 gatggctata atggaaaaga tattattgaa ataggtaaag atttagcaga gaaacaccct
     2581 gaaattaaag attattctga agaagcacgt ttgaaagaat ttagaaaatt aggcgtagaa
     2641 tacgaaatgg ctaaattgaa aaatgattta gcagagttca atacgcattt tgataattgg
     2701 tttagtgaaa catctttata tgaaaaatgc gaaattcttg aagttttagc aaaaatgaaa
     2761 gaattaggtt atacgtatga agctgatggc gctacatggt tacgtacaac tgattttaaa
     2821 gacgacaaag acagagtctt aattaaaaat gacggtacat atacgtattt cttaccagat
     2881 attgcgtacc acttcgataa agttaaacgt ggtaatgaca ttttaatcga tttatttggt
     2941 gctgatcatc atggttatat taatcgtttg aaagcatctc ttgaaacgtt tggtgtagat
     3001 agtaatcgtt tagaaattca aatcatgcaa atggttcgtt taatggaaaa tggtaaagaa
     3061 gtgaagatga gtaaacgtac tggtaatgcg attacattaa gagaaattat ggacgaagtt
     3121 ggcgttgacg ctgcacgtta tttcttaact atgcgtagtc ctgatagtca ctttgatttt
     3181 gatatggaat tagcgaaaga gcaatctcaa gacaatccag tttactatgc tcaatatgca
     3241 catgcgcgta tttgttcaat tttaaaacaa gcgaaagagc aaggtattga agtgactgct
     3301 gcgaatgatt ttacaacaat tactaatgaa aaagcgattg aattgttgaa aaaagtagct
     3361 gactttgagc ctacaattga aagtgctgca gaacatagat cagcacatag aattactaat
     3421 tatattcaag atttagcttc tcatttccat aaattctaca atgctgaaaa agtgttaaca
     3481 gatgatattg aaaaaacaaa agcacatgtt gctatgattg aagcggtcag aattacattg
     3541 aaaaatgcat tggcaatggt cggtgtaagc gcacctgaat caatgtaaga acatttatat
     3601 acactccaac gtagagtttc tcgaaagata ctttgtgttg gagtgttttt aattaggtat
     3661 gtgacatatt ggggaatgct tagtatgtga ataaggttaa gaggaacgca gttggatgct
     3721 actgcacaac tgcataagag agcctgagac ataaatcaat gttctatgct ctacaaagtt
     3781 ataatggcag tagttgactg aacgaaaatt cgcttgtaac aagctttttt caattctagt
     3841 caaccttgcc ggcggggccc caacaaagag aaattggatt cccaatttct acagacaatg
     3901 caagttgggg tgggacgacg aaataaattt tacgataata tcatttctgt cccactccct
     3961 ctaaaatgga gggtgtaaat gttaggaact gatgaattat ataaagtttt atatgaacat
     4021 ctcggaccac aattttggtg gcctgctgat aatgacattg aaatgatgtt aggtgcaatt
     4081 ttagttcaaa atactagatg gcgaaatgca gaaattgcat tgaatcaagt taaagaacac
     4141 acgcatttta atccaaatca tatattagaa ctacctattg aaacgttaca atcattgata
     4201 cattcaagtg gcttttataa aagtaaatca ctgacgatta aaacattatt aacatggtta
     4261 gcacgacatc atttcaatta tcaagagatt aatgagcgat ataaaggtgg attaagaaaa
     4321 gaattattat ctttgaaagg tattggaagt gaaacagcag atgtcttact tgtttatata
     4381 ttcggacgta ttgaatttat tccagatagc tatacaagaa aaatatatga taaattagga
     4441 tatgaaaaca ctaaaaatta tgatcaatta aaaaaagtag tcacattacc aaatcatttt
     4501 acaaatcaag atgctaatga atttcatgct ctgttagatg tatttggtaa acattacttt
     4561 agagacaaag atataaagaa ttatgatttt ttagaacctt actttaaaaa gtaaacgctg
     4621 tgaagttaga tagatgagtt tatatgaaat ataaaaaata atttactatt ttcttttagt
     4681 atgtggactt atataataaa tagaagcata taaagtaaaa aacagttgga tgctaccgcg
     4741 caactgcata aaagcctcta atcgctaaag gtgaagagga acgcagttgg cttgcttgcg
     4801 cacaactgca taaaagcctc taatcgctaa agctcaagag gctttaaaaa ggagatgtga
     4861 tagtgtgaag aaatcgttaa ttgcttttat tttgattttt atgcttgtcc tgagtggctg
     4921 tggtatgaaa gataatgata aacaaggtag caatgataat ggctcgtcta aatcgccgta
     4981 ccatagaatt gtttcgttaa tgcctagtaa tactgaaatt ttatatgaat taggattagg
     5041 taaatacata gttggtgttt caacggttga tgattatcca aaagatgtga aagagggtaa
     5101 gaaacaattt gatgctttga atctaaataa agaggaactt ttaaaggaaa agccagatct
     5161 aattcttgcg catgagtcgc aaaaggcaac tgctaataaa gtattgtcat cattagagaa
     5221 acaaggcatc aaagtagtgt atgttaaaga tgcacaatca attgatgaaa catacaacac
     5281 atttaagcaa attgggaaat taacgcatca tgataagcag gctgaacaac ttgttgagga
     5341 aactaaagat aatatcgata aagtcataga ttcaattcct gctcatcata aaaaatcaaa
     5401 agtatttatt gaggtttcat caaagcctga aatatataca gcaggaaagc atacattttt
     5461 caatgatatg ttagaaaaat tagaagccca aaatgtttat agtgacatta atggttggaa
     5521 ccctgtaacg aaggaaagta ttattaaaaa gaacccagat atattaattt cgacggaagc
     5581 taagacaaga tcagattata tggatatcat caaaaaaaga ggtggattca ataaaattaa
     5641 tgctgtcaag aatacacgta ttgaagttgt aaatggtgat gaagtatcaa gacccggtcc
     5701 acgtattgat gaaggattaa aagaattaag agatgcaatt tatagaaaat aaaccattct
     5761 aattatgccc cttattgcta catgtaaaaa aatacatgtt tgagataagg ggtttttaaa
     5821 atatatttag tgaatgatag caacgcgagt atgtgattgc tataatgaat gtaattatcg
     5881 atgaaagaaa agagaatgct atgacattta ataaagtatt attgagctgg atagtcatat
     5941 tgattataac aactagcata tatctatttt ggcagttggg ggatatcaat gatgtattta
     6001 accagtctat tttaatcaat gttagattac cgagattatt agaagcattg ttgacaggta
     6061 tgatattaac agttgcaggt cttatatttc aaacagtttt aaataatgca ttggcagata
     6121 gctttacatt aggattggca agcggtgcta catttggttc aggattagca ttatttttag
     6181 gtttaacaac gttatggatt cctgtatttt caataacatt tagtttgata acattaataa
     6241 ctgtattagt cattacgtcg gtattgagcc aaggctatcc agttagaatc ttaatattaa
     6301 gtggtttaat gattggtgca ttattcaatt cacttctata ttttttgatt ttattaaaac
     6361 ctcgcaaatt aaatacaatt gccaattatt tgtttggtgg ctttggtgat gcagaatact
     6421 caaatgtatc cataatagca atcacattta tcattgcact gtttggtata tttatcattc
     6481 ttaatcaact aaagttattg caattaggag aactaaaaag tcagtcacta ggcttaaatg
     6541 ttcaattgat tacatatatc gcgttatgta tagcttctat gataacggcg ataaatgtcg
     6601 catatgttgg catcattgga ttcattggta tggtaatacc gcaactcatt agaaaatggc
     6661 agtggaaaca atcattagga agacaattgg ctttgaatat tgtaattgga ggacaaataa
     6721 tggttatggc agattttatt ggtagccata tattgtcacc agtacaaata ccggcaagta
     6781 ttatcattgc attaattggt ataccagtgt tattttacat gctaatatct cagtcgaaac
     6841 ggttacacta gcacatgaca tttgctaaaa taaaaataac tataaacata aagagggcat
     6901 aagcgatgga tttgaatcaa attaaagcag ttgtatttga tttagaaggt acgttgttgg
     6961 acagagttaa atctcgagag aaatttatcg aagagcaata tgaacgattt catgactact
     7021 taattcatgt tcaactggca gattttaaaa aagcatttat tgagctagat gacgatgaag
     7081 ataatgataa acctgattta tataaagaaa tcattaaacg tttccatgta gataggttaa
     7141 cttggaaaga cttatttaat gattttgaaa tgcattttta tcgttatgta tttccttatt
     7201 acgatacttt gtatacacta gaaaagctat cgcaaaaagg ctttcaaatt ggtgttatcg
     7261 caaatggtaa atctaagatt aaacaatttc gattacattc acttggtttg atgcatgtta
     7321 ttaattattt atcaacatca gaaacagttg gttttcgtaa accacatcct aaaatttttg
     7381 aagatatgat tgatcaacta ggggtattac ctgagcaaat tatgtatgtt ggcgatgatg
     7441 cgttaaatga tgtagctcca gcacgagcta tgggcatggt tagtgtatgg tataaacaag
     7501 aagatgctga aattgaacca ctcgaagaag aagttgattt tacaattaca acagtggaag
     7561 aattattaac cattttacca ataaaaaatg ataataaagg agaaaattat ggatctattt
     7621 actagaaaag atggaacatc gattcattac agtacattag gtgaaggcta tcctatcgta
     7681 ttgattcata ctgtacttga taattattct gtgtttaata aattagcagc acaattagca
     7741 aaatcatttc aagttgtgtt aattgattta cgtggacatg gctattctga taaacctcgt
     7801 cacattgaaa taaaagattt ttctgatgac attgttgaat tacttaaata tttatacatt
     7861 gaagaagttg catttgtatg ccatgaaatg ggtggaatca ttggtgcgga tatttcagta
     7921 cgttatcctg aatttacatc atcacttatg ttggtaaatc caacatctat tgaaggtgaa
     7981 ttaccggaag aacgtttatt tagaaaatat gcccatatta ttcgaaactg ggatcctgaa
     8041 aaacaagata aatttttaaa taagcgtaag tattatcgtc cgagaaaaat gaatcgattc
     8101 cttaaacatg tcgtagatac aaatgaaata tcaactaaag aagaaattca agcagttaaa
     8161 gaggtattca aaaacgctga tatttctcaa acttatagaa atgtcgtagt accgacaaaa
     8221 attattgcag gagaattcgg tgaaagaaca acaagattgg aagctaaaga agtagctgat
     8281 ttaatccaaa atgcggactt tgaagtatat caagaatcaa gtgcattccc atttgttgaa
     8341 gagcaagaaa gattcgtcga agatacagct tcatttatca acaaacatca cgatgaaaag
     8401 catgtttaat ttttagtgat aagtgaaata taaaagtagt aattattatt tgtaatataa
     8461 ttgtaatatg actgttgttt tagaaatgat tgttgtataa tggttgtgag cacaagacga
     8521 tataaataaa aggagagagt gcttatgaaa aagctactaa cggcaagtat aattgcatgt
     8581 tctgttgtaa tgggagtagg cttagtgaac actagtgccg aagcagcaag tggcaactct
     8641 attgatactg ttaaacaatt aattaagggt gatcagtcat tagaaaatgt gaaaattggc
     8701 gaatctatta aagatgtttt aactaagtac aaaaatccta tgtattctta caatgaagat
     8761 ggaactgaac attattacga attccatact aaaaaaggta tgttattagt aactactgat
     8821 ggtaagaaaa acaatggtaa agtaactcat atttcaatga tgtataatga tgctaatggt
     8881 ccaacatatc aagctgttaa aaattatgtt ggcaaagcag taacacatac ggaatatagc
     8941 aaagttgctg gtaattttgg atatattgaa aaaggcaaaa cgacttatca atttgcctca
     9001 gcaccaaaag ataaaaacat aaaattatat cgtattgatt tggaaaaata ataattaaat
     9061 taatcgtgct taaccgacaa gttaaatcgc aatacttgtc ggttatttta ttacatggca
     9121 taatacagtg aatattaatt tttatgattt gcgaagggga aattaaacca atacaataaa
     9181 taaagatgtt tcattttaaa ggagtactac caatgcgtat t
//
LOCUS       DAMZKC010000103         9077 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.104, whole genome shotgun sequence.
ACCESSION   DAMZKC010000103 DAMZKC010000000
VERSION     DAMZKC010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9077
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.104"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(137..973)
                     /locus_tag="POB55_001820"
     CDS             complement(137..973)
                     /locus_tag="POB55_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HDI6500780.1"
                     /translation="MDLASKYFNGVNWRYIDHSSGLEPMQSFAFDDTFCESVGKDISD
                     NVVRTWIHQHTVILGIHDSRLPFLKDGIDYLTNEIGYNAIVRNSGGLGVVLDQGVLNI
                     SLMFKGQTETTIDEAFTVMYLLISKMFENENVDIDTMEIEHSYCPGKFDLSIDGKKFA
                     GISQRRVRGGIAVQIYLCVEGSGSERALMMQTFYEHALKGEVTKFKYPEIEPSCMASL
                     ETLLNKTITVQDVMFLLLYAIKDLGGVLNMTPITQEEWQRYDTYFDKMIERNKKMIDQ
                     MQ"
     gene            complement(976..1962)
                     /gene="pta"
                     /locus_tag="POB55_001821"
     CDS             complement(976..1962)
                     /gene="pta"
                     /locus_tag="POB55_001821"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HDI6500781.1"
                     /translation="MADLLNVLKDKLSGKNVKIVLPEGEDERVLTAATQLQATDYVTP
                     IVLGDETKVQSLAQKLDLDISNIELINPATSELKAELVQSFVERRKGKATEEQAQELL
                     NNVNYFGTMLVYAGKADGLVSGAAHSTGDTVRPALQIIKTKPGVSRTSGIFFMIKGDV
                     QYIFGDCAINPELDSQGLAEIAVESAKSALSFGMDPKVAMLSFSTKGSAKSDDVTKVQ
                     EAVKLAQQKAEEEKLEAIIDGEFQFDAAIVPGVAEKKAPGAKLQGDANVFVFPSLEAG
                     NIGYKIAQRLGGYDAVGPVLQGLNSPVNDLSRGCSIEDVYNLSIITAAQALQ"
     gene            2130..2882
                     /locus_tag="POB55_001822"
     CDS             2130..2882
                     /locus_tag="POB55_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-dependent peroxidase"
                     /protein_id="HDI6500782.1"
                     /translation="MSQAAETLDGWYSLHLFYAVDWASLRLVPKDERDALVTEFQSFL
                     ENTATVRSSKSGDQAIYNITGQKADLLLWFLRPEMKSLNHIENEFNKLRIADFLIPTY
                     SYVSVIELSNYLAGKSDEDPYENPHIKARLYPELPHSDYICFYPMNKRRNETYNWYML
                     TMEERQKLMYDHGMIGRKYAGKIKQFITGSVGFDDFEWGVTLFSDDVLQFKKIVYEMR
                     FDETTARYGEFGSFFVGHLINTNEFDQFFAIS"
     gene            3431..4195
                     /locus_tag="POB55_001823"
     CDS             3431..4195
                     /locus_tag="POB55_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HDI6500783.1"
                     /translation="MDINSEEYKQEVLIKDVVMLAARILLESGAEGTRVEDTMTRIAK
                     KLGYSESNSFVTNTVIQFTLHSESFPRIFRITSRDTNLIKISQANKISRQITNNEISL
                     AEAKTQLEKIYVAKRDSSLPFKGFAAAMIAMSFLYLQGGRLIDVLTAILAGSLGYLVT
                     EILDRKLHAQFIPEFIGSLVIGIIAVIGHTLIPTGDLATIIIAAVMPIVPGVLITNAI
                     QDLFGGHMLMFTTKSLEALVTAFGIGAGVGSVLILV"
     gene            4208..4660
                     /locus_tag="POB55_001824"
     CDS             4208..4660
                     /locus_tag="POB55_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDI6500784.1"
                     /translation="MFWILNFIFSFLASMFFCVIFDAPRKLYLSCGFVGTCGWMVYTL
                     FFNGFNVHTIYSSFFGSLALGLLSHYMARKQKEPAIIFMVTGIIPLVPGGLAYDATKN
                     LVLLNFSTAINTMLEVTLIAGAIALGLLFADQISKLIVSGFVKSFKRL"
     gene            complement(4801..6285)
                     /locus_tag="POB55_001825"
     CDS             complement(4801..6285)
                     /locus_tag="POB55_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HDI6500785.1"
                     /translation="MEKKNKQIDRGDLKQNLSEKFVWAIAYGSCIGWGAFILPGDWIK
                     QSGPIAASIGIVIGTLLMILIAVSYGALVERFPVSGGAFAFSFLSFGRYVSFFSSWFL
                     TFGYVCVVALNATAFSLLVKFLLPDVLNNGKLYTIAGWDVYITEIIIATVLLLVFMLV
                     TIRGASVSGSLQYYFCVAMVIVVLLMFFGSFFGNNFALENLQPLAEPSKGWLVSIVVI
                     VSVAPWAYVGFDNIPQTAEEFNFAPNKTFKLIVYSLLAASLTYVVMILYTGWLSTSHQ
                     SLNGQLWLTGAVTQTAFGYIGLGVLAIAIMMGIFTGLNGFLMSSSRLLFSMGRSGIMP
                     TMFSKLHSKYKTPYVAIIFLVGVSLIAPWLGRTALTWIVDMSSTGVSIAYFITCLSAA
                     KLFSYNKQSNTYAPVYKTFAIIGSFVSFIFLALLLVPGSPAALTAPSYIALLGWLIIG
                     LIFFVIRYPKLKNMDNDELSRLILNRSENEVDDMIEEPEKEKTK"
     gene            complement(6406..6774)
                     /locus_tag="POB55_001826"
     CDS             complement(6406..6774)
                     /locus_tag="POB55_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HDI6500786.1"
                     /translation="MKLFIILGALNAMMAVGTGAFGAHGLQGKISDHYLSVWEKATTY
                     QMYHGLALLIIGVISGTTSINVNWAGWLIFAGIIFFSGSLYILVLTQIKVLGAITPIG
                     GVLFIIGWIMLIIATFKFAG"
     gene            complement(6907..7287)
                     /locus_tag="POB55_001827"
     CDS             complement(6907..7287)
                     /locus_tag="POB55_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5327 family protein"
                     /protein_id="HDI6500787.1"
                     /translation="MLNRETLIARIEQELVQAEQAQHDHDFEKHMYAIHILTSLYAST
                     SNTPHIGEQQMNRRIANHNQMPQSQITQPTHQVTAAEIEAMGGKVNTHSAHHHNKSYS
                     QPSNQQQRLATDDDIGNGESIFDF"
     gene            complement(7288..7944)
                     /locus_tag="POB55_001828"
     CDS             complement(7288..7944)
                     /locus_tag="POB55_001828"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDI6500788.1"
                     /translation="MEWSQIFHDITTKHDFKAMHDFLEKEYSTAIVYPDRENIYQAFD
                     LTPFENIKVVILGQDPYHGPNQAHGLAFSVQPNAKFPPSLRNMYKELADDIGCVRQTP
                     HLQDWAREGVLLLNTVLTVRQGEANSHRDIGWETFTDEIIKAVSDYKEHVVFILWGKP
                     AQQKIKLIDTSKHCIIKSVHPSPLSAYRGFFGSKPYSKANAYLESVGKLPINWCESEA
                     "
     gene            8128..8958
                     /gene="thiD"
                     /locus_tag="POB55_001829"
     CDS             8128..8958
                     /gene="thiD"
                     /locus_tag="POB55_001829"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HDI6500789.1"
                     /translation="MALKKVLTIAGSDTSAGAGMQADLKTFQELDTYGMVALTAIVTM
                     DKDTWSHDVTPLPMDVFEKQLETALSIGPDAIKTGMLGTEEIIKRAGEVYEASNAQYF
                     VVDPVMVCKGEDEVLNPGNTEAMIKYLLPKATVVTPNLFEAGQLSGLGKLNSIEDMKK
                     AATIIFDKGAQHVIIKGGKALDQDKSYDLYYDGQTFYQLTTDMFQQSYNHGAGCTFAA
                     ATTAYLANGKSPKEAVISAKAFVASAIKNGWKMNDFVGPVDHGAYNRIEHIDVEVTEV
                     "
BASE COUNT         2953 a         1637 c         1447 g         3040 t
ORIGIN      
        1 ttccatcata tcattacttt taatttaatg atgttcaatt taaatattag gtcaataaca
       61 tatttatgct ttttatggat actttcaaaa ataacagccc caaacgataa cttgaaaggg
      121 gctgttaaat atttaactat tgcatttgat ctatcatttt cttgtttctt tcaatcattt
      181 tatcaaaata cgtatcgtat ctttgccatt cttcttgagt aattggcgtc atatttaata
      241 caccgccaag atctttgatt gcatataata gtaaaaacat tacatcttga acagtaatcg
      301 ttttgttaag caatgtctct aatgaggcca tacaagatgg ttcaatttca ggatatttaa
      361 atttagtcac ttcacctttt aaagcatgtt cataaaatgt ttgcatcatc aatgcacgtt
      421 ctgaaccaga gccttcaaca caaagataaa tttgtacagc aataccgcct ctaactcttc
      481 gttgcgatat gcctgcaaat ttcttaccat cgatacttaa gtcaaatttt cctgggcaat
      541 aagaatgttc aatttccatc gtatcaatat caacattctc attttcgaac attttgctaa
      601 ttaagaggta catcacagta aacgcttcat caatcgttgt ttctgtttgt cctttgaaca
      661 tcagcgatat atttaataca ccttgatcta gaacgacacc taagccacca gaatttctaa
      721 caatggcatt ataaccaatc tcattcgtta aataatcaat gccatctttt aaaaacggca
      781 atcttgaatc atgaatacca agaataacag tatgttgatg aatccaagta cgcacaacat
      841 tatctgatat atctttgccc acactttcgc aaaatgtatc atcgaatgcg aaagattgca
      901 taggttctaa tccagaagaa tgatcgatat atcgccagtt gacgccatta aaatatttac
      961 tcgctaaatc catcgttatt gtaaggcttg cgctgctgtg ataattgaaa gattgtatac
     1021 atcttcaatt gagcagccac gtgacaagtc atttactgga gaatttaaac cttgtaatac
     1081 tggaccaact gcatcatatc cacctaaacg ttgtgcaatt ttgtaaccaa tattaccagc
     1141 ttctaaactt gggaatacaa agacatttgc atcaccttgt aatttagcac ctggcgcttt
     1201 tttctcagca acacctggta caatcgcagc atcaaattgg aattcgccat caatgattgc
     1261 ttctaatttt tcttcttcag ctttttgttg tgctaatttg acagcttctt gaacttttgt
     1321 cacgtcgtct gatttagcag acccttttgt tgaaaagctt aacattgcaa cttttggatc
     1381 catgccaaag cttaatgctg attttgcact ttctactgca atttctgcaa gtccttgtga
     1441 atcaagttct ggattgattg cacaatcacc aaagatgtat tgtacatcac ctttaatcat
     1501 aaagaagata cctgatgttc ttgatacacc tggtttcgtt ttgatgattt gtaaagctgg
     1561 acgcacagtg tctcctgttg aatgtgctgc accactaact aaaccatctg ctttaccagc
     1621 ataaacaagc attgtaccga agtagttcac attgtttaat aattcttgtg cttgttcttc
     1681 agtcgcttta cctttacgtc gttcaacaaa tgattgaact aattcagctt tcaattcact
     1741 tgtcgcagga ttaattaatt caatattaga aatatcaaga tcaagttttt gcgctaaaga
     1801 ttgaacctta gtctcatcac ctaacacgat tggtgtaaca taatctgttg cttgtaattg
     1861 tgttgcagct gttagaacac gttcgtcctc tccttcaggt aatacgattt taacgttttt
     1921 accagaaagt ttgtctttta atacatttaa taaatcagcc ataatgtcct cctgtaatat
     1981 aaatcttatt aatcattcac ggtataatta tacgccattt tatttcataa tgccacgtca
     2041 ccaatttgta atcataattt tatcgttacc cctttgcttt atgataaaat ttataaagaa
     2101 ctgatgattt ttgaaaagga gcgataaaca tgagtcaagc agccgaaaca ttagatggtt
     2161 ggtatagtct acatttattt tatgcagttg attgggcatc attacgtcta gttccaaagg
     2221 acgaacgcga tgcacttgtc actgaatttc aatcattttt agaaaataca gcaactgtaa
     2281 gatcatcaaa atctggtgat caagctattt ataatataac tggtcaaaaa gcagatttgt
     2341 tattatggtt cttacgtcct gaaatgaagt ctttaaatca tattgaaaat gaatttaaca
     2401 aattgcgcat tgctgacttc ttaatcccta catattcata tgtatcagtc attgaattga
     2461 gcaattattt agctggtaaa tctgatgaag atccttatga gaaccctcat atcaaagcaa
     2521 gattataccc agaattacca cattctgatt atatttgttt ctatccaatg aacaaacgtc
     2581 gtaatgaaac ttataactgg tacatgttaa ctatggaaga acgccaaaaa ttaatgtatg
     2641 accatggtat gattggtaga aaatatgctg gcaaaatcaa acaatttatt actggttctg
     2701 tagggtttga tgatttcgaa tggggcgtaa cattgttctc agatgacgta ttacaattca
     2761 aaaaaattgt atacgaaatg cgctttgatg aaacaacagc acgatacggt gaattcggta
     2821 gtttctttgt aggacatctt attaacacaa acgaattcga tcaattcttt gcgatttctt
     2881 aatacattgg tacgtttata aattaataaa aaaattccaa gcttatcggt ttaagcttgg
     2941 aatttttcgt ttatcttcag tatattcccg tatacataag acgtgatttg gtaaatagtt
     3001 gaaatctgta tgtttaaact tatatatatg tgctaatgta ttatcaataa caaagtacac
     3061 tttgctcata gcaaagtgta cccgagtagt cttccttggg agaactttaa ctactatcac
     3121 tacatataaa cgttaacctc aatagaaatt atacagtcgc tactctatac aatttttgta
     3181 atggttaact aatattattt taacctattt gaaatatttg aaacatattt ttgtcgaatt
     3241 tttttcaata atttttcctt tttatacttc aagagaattt taactactaa aaattccgat
     3301 gattattatt acaatagtat caaatattag ttttttaaaa tcaataacaa cttatcaaaa
     3361 agctcatgtg gttattttat agtgtataaa ctataatgag tattaaattc ttataaacaa
     3421 tggtgatgaa atggacataa attcagaaga atacaaacaa gaggtactta tcaaagacgt
     3481 tgtcatgctt gctgctcgca tactattaga atctggtgca gaaggtacgc gtgtagaaga
     3541 taccatgaca cgtattgcaa aaaaacttgg ttacagtgaa agtaacagct ttgttacaaa
     3601 cactgtcatc cagtttacgt tacattcgga atcgtttcct agaatattta gaattacctc
     3661 tcgagataca aacttaataa aaatttcgca agctaataaa atttcgcgtc aaattacaaa
     3721 caatgaaatt tctttagccg aagcaaaaac gcaacttgaa aaaatatatg ttgctaagcg
     3781 tgacagcagt cttcccttta aaggttttgc tgcagcaatg attgcaatga gtttcttata
     3841 tttacaaggt ggtagattga ttgatgtttt aactgcgata ttagcaggta gtctaggata
     3901 cctagtcact gagattttag atcgcaagtt acatgcacag tttatcccag aattcattgg
     3961 ttcattagtt attgggatta tcgccgttat tggacataca cttatcccaa caggtgactt
     4021 ggcaactatt atcattgcag cagtcatgcc tattgttcct ggtgtattaa taacaaacgc
     4081 aatacaagat ttatttggtg gacacatgtt gatgttcaca acgaaatcat tagaagcatt
     4141 ggttactgcg tttggcatcg gtgctggcgt tggtagcgta ttaattttag tataggagta
     4201 tcagactatg ttttggatct taaactttat ctttagcttt ttagcttcaa tgttcttctg
     4261 cgtcattttt gatgcaccta gaaaattata tctatcatgt ggtttcgttg gtacatgtgg
     4321 atggatggtt tacaccttat tcttcaacgg ctttaatgtg cacactatat actctagttt
     4381 ctttggtagt ttagcattag gcttgttaag tcattatatg gctcgtaaac aaaaagaacc
     4441 tgccatcatt tttatggtaa cgggtatcat tccattagta cctggtggct tagcatacga
     4501 tgctacaaaa aatttagtct tattaaattt cagcacagca ataaatacaa tgttagaagt
     4561 aacacttatt gcaggagcca tcgcattagg tttattattc gccgaccaaa tttctaaatt
     4621 aattgtttct gggttcgtga aatcttttaa acgattataa atattgttag aattggctca
     4681 gcccaaacta ttagcaatta tatataaggt tcagctgcct cgtcttaaat gtcaatttat
     4741 aatacaaaaa atcgcacgat acgcctccga atgaagaagg tatattgtgc gattctttta
     4801 ttatttagtt ttttcttttt caggttcttc aatcatatca tcaacttcat tttcacttct
     4861 atttaaaatc aagcgactta attcatcatt atccatattt ttcaatttag gatatcgaat
     4921 cacaaagaat attaaaccga tgattaacca tccaagtaat gcaatataag acggtgcagt
     4981 cagtgctgca ggagaacctg gcactaataa caacgctaag aaaatgaatg atacaaatga
     5041 gccgataata gcaaacgttt tgtaaaccgg tgcatacgta ttactttgtt tgttataact
     5101 gaataatttc gctgcagaca aacatgtaat aaagtaggca atggatacac ctgtagatga
     5161 catatctaca atccaagtca atgcagttct tcctagccaa ggtgcaatta acgacacacc
     5221 tactaggaat atgattgcga catatggtgt tttgtattta ctatgtaatt tactaaacat
     5281 tgttggcata atacctgaac gtcccataga aaataacaag cgacttgaac tcatcaagaa
     5341 tccatttaaa ccagtaaata tacccatcat aattgcaatt gctaatacac ctaatccaat
     5401 ataaccaaat gctgtttgtg taacagcacc tgttaaccac aactgcccat ttaaactttg
     5461 atgacttgtt gataaccaac cagtgtataa aatcatgaca acataagtta atgatgctgc
     5521 taataaactg tacacgataa gcttaaatgt cttgtttggt gcaaagttaa actcttctgc
     5581 tgtttgtgga atattatcaa atccaacata tgcccatggt gccacggata caataaccac
     5641 aatagacact aaccatcctt tgctaggttc agctaacggt tgtaaatttt caagtgcaaa
     5701 attattacca aagaatgaac caaagaacat caataatacg acgattacca tcgccacaca
     5761 gaaataatat tgtaatgatc cagatacact tgcgccacga atcgttacta gcatgaatac
     5821 aagtagtaat acggtcgcaa taatgatttc cgtaatataa acgtcccagc ccgcaatggt
     5881 gtatagtttc ccattattta agacatctgg caataagaat ttaactagta aactgaatgc
     5941 ggtcgcattt aaagcaacga cacagacata accaaaagtt aaaaaccatg atgagaagaa
     6001 actcacatat ctgccgaaac ttaagaaact aaaggcaaac gcgccccctg atactggaaa
     6061 tctctctact aatgcgccat aactaaccgc aattaatatc attaataatg taccaataac
     6121 tataccaatt gatgctgcaa tcggacctga ctgcttaatc cagtctcctg gtaagatgaa
     6181 tgcgccccat ccgatacatg aaccatatgc aatcgcccat acaaactttt cagataggtt
     6241 ttgttttaaa tcgcctctat ctatttgctt attctttttt tccataaata aaaactcacc
     6301 tcgaaggtat tctataccca aaagatgagt ttgtaactct ttttcttgat tatttttatt
     6361 aaaaatttaa tgtttagtta cataggtaat ctaaagtttt aaaatttaac cagcaaattt
     6421 gaatgtcgca atgattaaca ttatccatcc aatgatgaac aatacgccac caattggcgt
     6481 aatcgcacct aaaactttaa tttgagttaa tactaaaata tataatgatc cactaaagaa
     6541 aataatacca gcaaatatta accagccagc ccagttaaca ttgattgaag ttgtaccact
     6601 aattacacct ataattaata atgctaagcc atggtacatt tgatacgtcg ttgctttttc
     6661 ccatactgat aaatagtgat cacttatttt tccttgtaaa ccatgcgcac caaatgcacc
     6721 tgttccgaca gccatcatcg cgtttaatgc acctaaaata ataaataatt tcattgctac
     6781 ctctccttat ataattcagt attaataaat gtttaaatcc gaacactcga ataaaagtat
     6841 atgcgtcttg attattaacc tacatactta acaagctatt aagtaattat gtttcattgt
     6901 tgctttttaa aaatcaaata tggattcacc attgccaatg tcatcatctg tcgctaatct
     6961 ttgttgttgg tttgaaggtt gtgaatatga cttattatga tgatgtgctg aatgcgtatt
     7021 tactttacca cccatcgctt caatttcagc agctgtcact tgatgagttg gctgcgttat
     7081 ttgtgattgt ggcatttgat tatggttagc aatacgacga ttcatttgtt gttcaccaat
     7141 atgtggtgta tttgatgttg aagcatataa agatgttaat atatgtatgg catacatatg
     7201 tttttcaaag tcatggtcat gctgtgcctg ctctgcttgt actaattctt gctcaattcg
     7261 tgctattaaa gtttctctat tcaacatcta cgcctcactt tcacaccaat taattggtaa
     7321 ttttcctact gactctaaat aggcattcgc tttggaatac ggttttgatc caaagaatcc
     7381 tctatatgca gacagtggac taggatgcac tgattttata atacaatgtt tagatgtatc
     7441 gataagcttt attttttgct gtgcaggttt cccccacaaa ataaagacaa catgttcttt
     7501 ataatcagac actgctttaa taatttcatc agtaaatgtt tcccaaccaa tatcacgatg
     7561 agaatttgct tcaccctgtc ttacggttaa aactgtattc aataacaaga cgccttctct
     7621 tgcccaatct tgtaaatgcg gtgtttgtct aacgcatcca atatcatctg ctaattcttt
     7681 atacatatta cgtaaagatg gagggaattt tgcgttaggt tgcactgaaa atgctaatcc
     7741 atgtgcttgg tttggaccat gatacgggtc ttgtcctaat ataacaactt tgatattttc
     7801 aaacggtgtt aaatcaaacg cttgatatat attttcccta tcagggtata cgattgcagt
     7861 cgaatattct ttttctaaaa aatcatgcat agctttaaag tcatgtttcg ttgttatgtc
     7921 atgaaaaatt tgcgaccatt ccattcaatc tcacctcagg acatatttta acatatcaca
     7981 cttatctaaa gtgaatgaca ttttcaaaag ttacaatagt atgaatgatt catttcaaca
     8041 atcatcaaat attgaatgct taatattttt caattaggtt aaaaatgata tgatgaatac
     8101 aatattatta tgtaggagtg aagctcaatg gctttaaaga aagttttaac aattgccggt
     8161 tctgacacaa gtgctggcgc aggtatgcaa gcagatttga aaacgttcca agaattagat
     8221 acgtatggca tggtcgcttt aactgccatc gttactatgg ataaagatac atggtcacac
     8281 gatgtcacac cattacctat ggacgtattt gaaaagcaac ttgaaacggc tttatcaatc
     8341 ggacctgatg ctattaaaac aggtatgtta ggtactgagg agataatcaa acgtgctgga
     8401 gaagtatatg aggcatctaa tgcacaatat tttgtagtag atcctgtcat ggtttgtaaa
     8461 ggcgaagatg aagtgcttaa tcctggaaat actgaggcga tgattaaata tttactacct
     8521 aaagcaacgg tagtgacacc aaacttattt gaagcgggtc aattatcagg attaggtaaa
     8581 ttaaattcaa ttgaagatat gaaaaaggct gcaacaatta tctttgataa aggcgcacag
     8641 catgtcatca ttaaaggtgg caaagcttta gatcaagata aatcgtatga cttatactat
     8701 gatggtcaaa cattttatca actaacaaca gacatgttcc aacaaagtta taaccatggt
     8761 gctggttgta catttgctgc ggcaacaact gcatatttag ctaacggtaa gtcaccgaaa
     8821 gaagctgtga ttagcgcgaa agctttcgtc gcttctgcta ttaaaaacgg ttggaaaatg
     8881 aatgattttg ttggtcctgt ggatcacggt gcatacaacc gtattgaaca tatcgatgtt
     8941 gaagtaacag aggtttaacg attaggcggt tcatctataa atcaacatta ttagccacac
     9001 cacttaaatt gggtatgaac tcaatttatg tgatgtggtt atttttttat aatcatcatg
     9061 ctctgtatat aatttaa
//
LOCUS       DAMZKC010000104         9026 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.105, whole genome shotgun sequence.
ACCESSION   DAMZKC010000104 DAMZKC010000000
VERSION     DAMZKC010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9026
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.105"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            144..743
                     /locus_tag="POB55_001830"
     CDS             144..743
                     /locus_tag="POB55_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HDI6500790.1"
                     /translation="MAFELPKLPYAFDALEPHFDKETMEIHHDRHHNTYVTKLNAAVE
                     GTDLESKSIEEIVANLDSVPANIQTAVRNNGGGHLNHSLFWELLSPNSEEKGTVVEKI
                     KEQWGSLEEFKKEFADKAAARFGSGWAWLVVNNGQLEIVTTPNQDNPLTEGKTPILGL
                     DVWEHAYYLKYQNKRPDYIGAFWNVVNWEKVDELYNATK"
     gene            864..2939
                     /locus_tag="POB55_001831"
     CDS             864..2939
                     /locus_tag="POB55_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HDI6500791.1"
                     /translation="MLKRLKEKSNDEIVQNTINKRINFIFGVIVFIFAVLVLRLGYLQ
                     IAQGSHYKQIIKNDENITVNESVPRGRILDRNGKVLVDNASKMAITYTRGRKTTQSEM
                     LDTAEKLSKLIKMDTKKITERDKKDFWIQLHPKKAKAMMTKEQAMLADGSIKQDQYDK
                     QLLSKIRKSQLDELSSKDLQVLAIFREMNAGTVLDPQMIKNEDVSEKEYAAVSQQLSK
                     LPGVNTSMDWDRKYPYGDTLRGIFGDVSTPAEGIPKELTEHYLSKGYSRNDRVGKSYL
                     EYQYEDVLRGKKKEMKYTTDKSGKVTSSEVLNPGARGQDLKLTIDIDLQKEVEALLDK
                     QIKKLRSQGAKDMDNAMMVVQNPKNGDILALAGKQINKSGKMTDYDIGTFTSQFAVGS
                     SVKGGTLLAGYQNKAIKVGETMVDEPLHFQGGLTKRSYFNKNGHVSINDKQALMHSSN
                     VYMFKTALKLAGDPYYSGMALPSDISSPAQKLRRGLNQVGLGVKTGIDLPNETRGQIE
                     PLTNNPGNYLDLSIGQYDTYTPLQLSQYVSTIANDGYRIQPHIGLTIHESTNKDEVGP
                     LKKKINGTVLNKVNNTEKEIKQIQEGFKMAFNDKDGTGYVSFKDTVVPTAGKTGTAEV
                     FQNGEPRVNSTYIGYAPIDDPKLAFSIVYTNQPVPPPWLTGGDLGRDVINYYFKQLGK
                     DDKNKDKDK"
     gene            3052..3201
                     /gene="rpmG"
                     /locus_tag="POB55_001832"
     CDS             3052..3201
                     /gene="rpmG"
                     /locus_tag="POB55_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDI6500792.1"
                     /translation="MRVNVTLACTECGDRNYITTKNKRNNPERVEMKKFCSRENKQTL
                     HRETK"
     gene            3410..3949
                     /locus_tag="POB55_001833"
     CDS             3410..3949
                     /locus_tag="POB55_001833"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HDI6500793.1"
                     /translation="MTKNEIRKYILHKMKNFNKAEKRKADTWLRNQFFATEEYKEANA
                     IALVLSFNHEVDTFSIIEQALMDHKRIFVPKMDYLNHQMTFKEIFNLKDIDVDNKGIY
                     YPTSKGETTNNLDLIVVPGVGFQDDGYRIGYGGGYYDRFLANYQTKTISLLYDFQITS
                     FEPESFDQPVDKLIIYQSA"
     gene            3961..5424
                     /locus_tag="POB55_001834"
     CDS             3961..5424
                     /locus_tag="POB55_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001652331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HDI6500794.1"
                     /translation="MNIDKQFWKTIYYWIRYLNFDIVSREKDDQEIWLAHKRKKQVVI
                     FKQHIKSTQEIRFDKAKVLEHKDEIANFISFEPQSFEFYYFTESEFSEEQLNEVSPIR
                     IKFNVIRHTKDLIKHMPNIFLARLISEDNDKKTYMFYKRKVLTDNFLDKYMQKFSPAT
                     YTIIFVNVLIWLCMILYLNNFSDVKLLDVGGLVHFNVVHGEWYRIVTSMFLHFSFEHI
                     LMNMLSLFIFGKIVEAIIGSWRMLTVYFIAGLFGNFVSLSFNTTTISVGASGAIFGLI
                     GSIFAMMYVSKTFNKKMLGQLLIALVILVGVSLFMSNINIVAHIGGFIGGLLITLIGY
                     YYKVNRNIFWILLIGMLVIFIALQIRIFTIKEDNIYNKLIKDDMTSGNYDNAQNIVKQ
                     TINKNYADDQTYYLSGMIMATINSKSEGMTEWERGLRMFPKSGLLNFELAIANRSLND
                     DEKALKYVRKALNADPKNADYINLEKELTKSNESKNK"
     gene            5405..5608
                     /locus_tag="POB55_001835"
     CDS             5405..5608
                     /locus_tag="POB55_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HDI6500795.1"
                     /translation="MSRKINNFYDVQQLLKSYGFLIYFKNPEDMYEMIQQEISSLYQY
                     ELLSKEEYLKCTLIINQRRNEQK"
     gene            5605..6591
                     /locus_tag="POB55_001836"
     CDS             5605..6591
                     /locus_tag="POB55_001836"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HDI6500796.1"
                     /translation="MTKIILAADVGGTTCKLGIFTPELEQLHKWSIHTDTSDSTGYTL
                     LKGIYDSFVEKVNENNYNFSNVLGVGIGVPGPVDFEKGTVNGAVNLYWPEKVNVREIF
                     EQFVDCPVYVDNDANIAALGEKHKGAGEGADDVVAITLGTGLGGGIISNGEIVHGHNG
                     SGAEIGHFRADFDQRFKCNCGRSGCIETVASATGVVNLVNFYYPKLTFRSSILELIKE
                     NKVTAKAVFDAAKAGDQFCIFITEKVANYIGYLCSIISVTSNPKYIVLGGGMSTAGPI
                     LIENIKTEYHNLTFAPAQFETEIVQAKLGNDAGITGAAGLIKTYVLDKEGVK"
     gene            6591..6920
                     /locus_tag="POB55_001837"
     CDS             6591..6920
                     /locus_tag="POB55_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HDI6500797.1"
                     /translation="MAIVDVVVIPVGTEGPSVSKYIADIQKKLQEYKAMGKIDFQLTP
                     MNTLIEGELSDVLEVVQVIHELPFDKGLSRVCTNIRIDDRRDKSRKMNDKLTSVQKHL
                     ENSGENL"
     gene            6917..7540
                     /locus_tag="POB55_001838"
     CDS             6917..7540
                     /locus_tag="POB55_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDI6500798.1"
                     /translation="MRISSLTLGLVDTNTYFIENDKAVILIDPSGESGKIIKKLNQIN
                     KPLKAILLTHAHFDHIGAVDDIVDRFDVPVYMHEAEFDFLKDPVKNGADKFKQYGLPI
                     ITSKVTPEKLNEGSTEIEGFKFNVLHTPGHSPGSLTYVFDEFAVVGDTLFNNGIGRTD
                     LYKGDYETLVDSIQDKIFELEGDLPLFPGHGPYTTVDDEQLNPFLHG"
     gene            7592..8566
                     /locus_tag="POB55_001839"
     CDS             7592..8566
                     /locus_tag="POB55_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HDI6500799.1"
                     /translation="MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQ
                     IGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYHFNKIYFLRISTLPLSLGQESCVI
                     RIVPQFFQQQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
                     VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVI
                     QASLSGHLVLTTLHATDCKGAILRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLV
                     CEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTMDKYI"
     gene            8538..>9026
                     /locus_tag="POB55_001840"
     CDS             8538..>9026
                     /locus_tag="POB55_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HDI6500800.1"
                     /translation="MKLQWINTFKLHSKKRQLSKAQQIDLLSNLCNLLKYGFTLYQSF
                     QFLNLQMTYKNKQLGTTILSEISNGAPCNQILSLIGYSDTIVMQVYLAERFGNIIDVL
                     EETVNYMKVNRKSEQRLLKTLQYPLILVSIFIAMIIILNLTVIPQFQQLYTSMNIQLS
                     SFQ"
BASE COUNT         3376 a         1191 c         1631 g         2828 t
ORIGIN      
        1 acaaaatgtt taattatttt tgatttgagt tatattaaat gagtagagaa aataataagt
       61 agtagaattt aagcaaattc tttgttgaac cagtcactgc ttgttattat ttacttacag
      121 acattttagg aggatgatta tttatggctt ttgaattacc aaaattacca tacgcatttg
      181 atgcattaga accacatttt gacaaagaaa ctatggaaat tcatcatgac agacatcata
      241 acacttatgt tacgaaatta aatgctgcag tagaaggtac agatttagaa tctaaatcta
      301 ttgaagaaat tgttgctaat ttagacagtg taccagctaa catccaaact gctgtacgta
      361 ataatggcgg tggacattta aaccattcat tattctggga gttactttca ccaaactcag
      421 aagaaaaagg tactgtagta gaaaaaatta aagaacaatg gggttcttta gaagaattta
      481 aaaaagaatt tgctgacaaa gcagctgcac gctttggttc aggttgggct tggttagtcg
      541 taaacaatgg ccagttagaa attgtgacta caccaaacca agataatcca ttaactgagg
      601 gtaaaacacc tattttaggt ttagacgtat gggaacacgc ttattaccta aaatatcaaa
      661 acaaacgccc tgactacatt ggcgcatttt ggaatgtagt taactgggaa aaagttgacg
      721 aattatataa tgcaacaaaa taaagtatga ttacttgttc ggtctcttaa atgagaccgt
      781 tatttttttg tcaaaaagat agaaataatt tctaaattca tatatgattt aaagtgaaag
      841 actttgaata gaggtaggta gttttgttaa aaagactaaa agaaaaatca aatgatgaaa
      901 tcgttcaaaa tacaattaac aagagaatta actttatatt tggtgtgatt gtatttattt
      961 ttgcagtact agtactacgt ttaggttatt tacaaatcgc acaaggctca cattataaac
     1021 aaattataaa aaatgatgaa aacattacag tgaatgagtc tgtgccaaga ggtcgtattt
     1081 tagacagaaa tgggaaagtt ttagttgata atgcttctaa aatggctatt acatatacta
     1141 ggggtcgaaa aacaacacaa tcggaaatgt tggatacggc tgaaaagtta tcaaagctaa
     1201 tcaagatgga tactaagaaa attacagaac gtgataagaa agatttctgg attcagttgc
     1261 atcctaaaaa agcaaaagca atgatgacaa aagaacaagc tatgttagca gatggaagta
     1321 ttaaacaaga tcaatatgat aaacaactgt tatcgaaaat cagaaaatca caattagatg
     1381 aattgtcttc taaagattta caagttttag ctatttttcg agagatgaat gcaggaacag
     1441 ttttagatcc acaaatgata aaaaatgaag atgtcagtga aaaagagtat gcagcagttt
     1501 ctcagcaact ttccaaatta ccaggtgtta acacgtctat ggattgggat agaaaatatc
     1561 catatggcga tactttaaga ggtatattcg gagatgtatc gacacctgct gaaggtattc
     1621 caaaagaatt gacagaacat tacttatcca aaggatattc acgcaatgat cgtgttggaa
     1681 aatcttacct agaatatcaa tatgaagatg tattgcgtgg taagaagaaa gaaatgaaat
     1741 acacaacgga caaatctggt aaagttacat cttcagaagt gttaaatcct ggcgctcgcg
     1801 gtcaagattt gaaattaacg atcgatatag atcttcaaaa agaagtagaa gcattattag
     1861 ataaacaaat taagaagctt cgcagtcaag gtgccaaaga tatggataat gcaatgatgg
     1921 ttgtacaaaa tcctaaaaat ggagacattc ttgcgcttgc cggaaagcag attaataaga
     1981 gtggtaaaat gactgattat gacattggta cgtttacttc tcaatttgcg gttggatctt
     2041 ctgtaaaagg tggaacatta ttagctggtt atcagaataa agctatcaaa gttggagaaa
     2101 caatggtcga tgaaccatta catttccaag gtggtttgac aaaacgatca tacttcaata
     2161 aaaacgggca tgtatctatt aatgataagc aagctttgat gcattcatca aacgtatata
     2221 tgtttaaaac agcattaaaa ttagcgggag acccttatta ttctggtatg gctttacctt
     2281 cagacataag ttcacctgcc caaaagctaa gaagaggatt aaatcaagta ggcttaggtg
     2341 tgaaaacagg gatagattta ccaaatgaaa caagaggtca aatcgaacca ttaacaaata
     2401 atccaggtaa ttatctagat ttatcaattg gtcaatatga tacctataca ccattacaat
     2461 tatcacaata tgtttcaact atagcgaatg atggttatag aatacagcca cacattggat
     2521 taacgattca tgaatcaact aataaagatg aggttggtcc actcaagaag aaaattaatg
     2581 gcactgtctt gaacaaggtt aataatactg aaaaggaaat caaacaaatt caagaaggat
     2641 tcaaaatggc atttaatgat aaagatggta ctggatatgt tagttttaaa gatacagtag
     2701 tacctactgc tggtaaaacg ggtaccgctg aagtgttcca aaacggagag ccaagagtta
     2761 actctactta tataggatac gcgccaattg atgatccaaa attagcgttt tcaattgtat
     2821 atacaaatca gcctgtacca ccaccatggt taacaggtgg agacttaggt agagatgtaa
     2881 ttaactacta ctttaagcag ttaggtaaag atgataaaaa taaagacaaa gacaaataaa
     2941 atttaacctg acgattgtgt agcgcatggt tgtaaaattt taactttgca aatattatag
     3001 atgttggtat aataataaag tcgtatttag aaatgataag gaggaataac catgcgcgta
     3061 aacgtaactt tagcttgtac ggaatgtggt gacagaaact acattacaac taagaacaaa
     3121 agaaataatc cagaacgtgt tgaaatgaag aaattctgtt cacgtgaaaa caaacaaact
     3181 ttacaccgtg aaacaaaata atcattaaaa ttaaatttaa attaacaata aaaaaggtga
     3241 aatctcaaaa taagaggttt caccttttta attatgcaca cgtatgaaat gataaaagat
     3301 tttcaattga aataataaat tttagaattg tcgcataatt catcatgaca acataatgac
     3361 atgtattgtt attaacgata taatagaatt gaataaagtg ggtgataatg tgactaaaaa
     3421 tgagattaga aaatacattt tacataaaat gaagaatttt aataaagctg aaaagcgaaa
     3481 agcagacaca tggttaagaa atcaattttt tgcaactgaa gaatacaaag aagcaaacgc
     3541 aattgcgcta gttctttctt ttaatcatga agtagatact ttttctatta ttgaacaagc
     3601 cttaatggat cataaacgta tttttgtacc gaaaatggat tatttaaatc atcaaatgac
     3661 ttttaaagaa atatttaatc tcaaagatat tgatgtcgat aataagggga tttactatcc
     3721 aacttcaaaa ggtgaaacaa cgaataacct agatttaatt gttgttcctg gtgttggatt
     3781 tcaagacgat ggatatagaa ttgggtatgg tggtggctat tacgacaggt ttttagctaa
     3841 ttatcagaca aagacaataa gcttattata cgattttcaa ataacatcat ttgaaccgga
     3901 atcattcgat caaccagtcg ataaattgat tatatatcaa tcagcatagt ggaggactaa
     3961 atgaacatag acaaacaatt ttggaaaaca atatattatt ggattaggta cttgaatttt
     4021 gatattgtca gtagggaaaa agatgaccaa gaaatttggt tagcccataa aagaaaaaaa
     4081 caagtagtca tttttaaaca acatatcaag tctacccaag aaatacgttt tgacaaagcg
     4141 aaagtgcttg aacacaaaga tgaaatagca aattttattt ctttcgaacc acaaagtttt
     4201 gaattttatt attttacaga atcggaattt tcagaagaac aattaaatga agtttcgcca
     4261 attagaatta aattcaatgt tataagacac acaaaagatt tgataaagca tatgccgaat
     4321 atatttttgg ctagacttat ttcagaagat aatgataaaa agacatatat gttctataaa
     4381 cgcaaagtat taaccgataa ctttttagat aaatatatgc agaaattttc accggcaaca
     4441 tacacaataa tatttgtaaa tgtcttaata tggttatgta tgattttata tttaaataat
     4501 ttttcggatg taaaattatt agatgttggc gggttagtgc attttaatgt cgtacatggt
     4561 gaatggtatc gaattgttac atcgatgttt ttacatttta gttttgaaca tatacttatg
     4621 aatatgcttt cattatttat ttttggtaaa atagtcgaag caattattgg ttcatggcgg
     4681 atgttaactg tatactttat tgcagggttg tttggaaact ttgtatcact atcatttaat
     4741 acgactacaa tttcagttgg ggctagtggt gctatatttg gtctgattgg atcaattttt
     4801 gcgatgatgt atgtttcaaa aacatttaac aaaaaaatgt taggacagtt attaattgca
     4861 ttagtgatat tagttggtgt ttctctgttt atgtcaaata taaatattgt ggcgcatatt
     4921 ggaggattca ttggtggttt attaataact ttaattggct attactataa agtgaatcgt
     4981 aatatttttt ggattttact aattggtatg cttgttatat ttattgcact tcaaattaga
     5041 atttttacaa ttaaagaaga taatatttat aataaattga tcaaagatga tatgactagt
     5101 ggtaattatg ataatgctca aaatattgtg aagcagacaa taaataaaaa ttatgccgat
     5161 gatcaaacat attatttaag tggtatgatt atggctacta tcaattctaa gtccgaaggt
     5221 atgacagaat gggagcgagg acttagaatg tttcctaaat caggtttatt aaattttgag
     5281 ttagcgatag caaatcgttc attaaatgat gatgaaaaag cattaaaata tgtgcgtaaa
     5341 gcattaaatg cagaccctaa aaatgcagat tatattaact tagaaaaaga gttgactaaa
     5401 tcaaatgagt cgaaaaataa ataactttta tgatgtacaa cagttattga aaagttacgg
     5461 atttctaata tattttaaaa atccagaaga tatgtacgaa atgattcaac aggagatttc
     5521 atcattgtat caatatgaac tgttatctaa ggaagaatat ttgaaatgta cgttgataat
     5581 taatcagaga aggaatgaac agaaatgaca aaaattattt tagcagctga tgtaggcggg
     5641 acgacttgta aattaggtat tttcacacct gaattagaac aattacataa atggtctatt
     5701 cacactgata catctgatag tacaggatat acacttttga aaggaattta tgattcgttt
     5761 gttgaaaaag taaatgaaaa taattataat ttttcaaatg tacttggcgt aggtattggt
     5821 gtaccaggtc ctgttgactt tgaaaaaggt acagtaaatg gagcagtaaa cttatattgg
     5881 ccagaaaaag ttaatgtacg tgagattttt gaacaattcg ttgattgtcc agtgtatgta
     5941 gataatgatg ctaacatagc tgctttaggg gagaaacaca aaggtgctgg tgaaggtgcc
     6001 gatgatgttg ttgccatcac acttggtaca ggtctaggtg gaggaattat ttcaaatggt
     6061 gaaatcgtac atggtcataa tggctctggc gcagaaatag gtcattttag agcagacttc
     6121 gatcaacgat ttaaatgtaa ttgtggtcgt tctggatgta ttgaaacagt tgcttcagcg
     6181 acaggcgttg ttaacttagt taacttctac tatccgaagt tgacgtttag atcttctata
     6241 ttagaattga ttaaagaaaa taaggttaca gcaaaagctg tttttgatgc ggcaaaagct
     6301 ggtgaccaat tctgtatttt cattactgaa aaggttgcaa actatattgg atatttatgt
     6361 agtattatta gtgttacaag taatccgaaa tatatcgttc taggtggagg aatgtctact
     6421 gcaggaccta ttttaattga aaatattaaa acagaatatc ataatttaac atttgcacct
     6481 gctcaatttg aaactgaaat tgtacaagcg aaattaggta atgatgcagg tattacagga
     6541 gcagcaggat taatcaagac ctatgtatta gataaagagg gggtaaaata atggctattg
     6601 ttgatgtggt tgttattcca gttggaacgg aaggtccgag tgttagtaaa tatattgcag
     6661 atattcagaa aaaacttcaa gaatataaag caatgggtaa aattgatttt caattaacac
     6721 caatgaatac tctaattgaa ggtgaattaa gcgatgtatt agaagttgtg caagtgatac
     6781 atgaattacc ttttgataaa ggtttaagta gagtttgtac aaatatccgt attgatgacc
     6841 gacgagacaa atctagaaaa atgaatgata aactaacatc agtacaaaaa catttagaaa
     6901 atagtggtga aaacctatga ggatttcaag cttaacttta ggcttagttg atactaatac
     6961 gtatttcatc gaaaatgaca aagctgttat tctgattgac ccttcaggtg aaagtggaaa
     7021 aattattaaa aaattaaacc aaataaataa accgttaaaa gctattttat taacacatgc
     7081 acactttgat catatcggag cagtcgatga tatagttgat cgattcgatg tcccggttta
     7141 tatgcatgaa gcagagtttg attttctaaa agatcccgtt aaaaatgggg cagataaatt
     7201 taagcaatat ggattaccaa ttattacaag taaggtaact cctgaaaagt taaacgaagg
     7261 tagcacagaa atagaaggat ttaagtttaa tgtgttacac acacctggac attcaccagg
     7321 aagtttaaca tatgtgttcg atgaattcgc agttgttgga gatacattat ttaataatgg
     7381 aatcggacgt acagatttat ataaaggtga ttatgaaacg ctagttgatt ctattcaaga
     7441 taaaatattt gaattagaag gcgatttacc tttattccct ggacatggtc catatacgac
     7501 ggttgatgat gaacaattaa atccattttt acacggttaa attgaagtaa taaaaacacc
     7561 tcctacgtga ttatatgtag gaggtgtttt tttgaagatt ctatttcaag aaataattaa
     7621 taaagcgata gaaatgaaag cgagtgatgt acattttatt ccagttaaaa atgaagtaag
     7681 tattaaattt agaattaatg ataacttgga gcagtatgaa caaattggga atagcattta
     7741 tcaaaagtta ttagtttata tgaagtttca agctgggctt gatgtttcta cacagcaagt
     7801 cgcacagagc ggtcgatata gttaccattt caataaaata tattttttga gaatatcaac
     7861 tttaccattg tcacttggcc aagaaagttg tgttatcaga attgtacctc aattttttca
     7921 acaacaaaaa tcaacttata aattcaatga ttttaaacac ctcatgaata agaaacaagg
     7981 attactattg tttagtgggc caactggttc aggaaagagt acattaatgt atcaaatggt
     8041 ctcatacgcg aataaagcct tgaatttaaa tgtaatttct atagaggatc ctgtagagat
     8101 gcaaattcct ggtatcgtcc aaattaatgt gaatgataaa gctggcatta actatgtaaa
     8161 ttcgtttaaa gctattttaa gatgtgatcc tgatgttatt ttaataggtg aaatcagaga
     8221 taaagatgtt gccaagtgtg ttatacaggc tagtttaagt ggtcaccttg ttctgactac
     8281 attgcatgca actgattgta aaggtgctat tttaaggcta ttagaaatgg gcatttctgt
     8341 acaagaattg atacaggcaa ctaacttaat tataaaccaa cgacttgtaa ctactattaa
     8401 gcaacagcga caattagtat gcgaaattct atctcagcaa caactccgat atttcttttc
     8461 ccataatcat tcattaccat catcatttaa gaacttagaa gataaacttg atgatatgac
     8521 aaaagcaggt gtcatttgtg aaactacaat ggataaatac atttaaacta cattctaaga
     8581 agcgacaatt aagtaaggcc caacaaatcg atttactttc aaatttatgt aatttgttga
     8641 aatatggttt cactctgtat caaagttttc aatttttaaa tcttcaaatg acatataaaa
     8701 ataagcaatt aggtaccacc attctaagtg aaatttcaaa tggtgcacca tgcaatcaaa
     8761 tattatcact gataggttat agcgatacta tcgtcatgca agtatatttg gcagaaagat
     8821 ttggcaatat tatagacgtt ctagaagaaa ccgtaaatta tatgaaagtg aatagaaagt
     8881 cagaacaacg attgttaaag acactgcaat accccttaat actagtttct atctttattg
     8941 ctatgattat tatattaaac ctcacagtaa ttccacagtt tcaacaatta tatacttcta
     9001 tgaatattca actatcttct tttcaa
//
LOCUS       DAMZKC010000105         9006 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.106, whole genome shotgun sequence.
ACCESSION   DAMZKC010000105 DAMZKC010000000
VERSION     DAMZKC010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9006
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.106"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..359
                     /locus_tag="POB55_001841"
     CDS             <1..359
                     /locus_tag="POB55_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HDI6500801.1"
                     /translation="EQYFRNLEFTCLQECINTADIIATGGGIIESEEAFNFLKNQKNI
                     IWLDCNIDIIYSRINDDPHRPNANNKTIKQLNDLYCSRNLRYNEIAFKKFDSHLLSIS
                     EIYYELLNLIKASDQY"
     regulatory      346..443
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            518..1609
                     /gene="gcvT"
                     /locus_tag="POB55_001842"
     CDS             518..1609
                     /gene="gcvT"
                     /locus_tag="POB55_001842"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HDI6500802.1"
                     /translation="MSSDLKQTPLYQNYVDRGAKIVEFGGWAMPVQFSSIKEEHNAVR
                     YEIGLFDVSHMGEIEVTGKDASQFVQYLLSNDTDNLTTSKALYTALCNEEGGIIDDLV
                     IYKLADDNYLLVVNAANTEKDFNWILKHKEKFDVEVQNVSNQYGQLAIQGPKARDLIN
                     QLVDEDVTEMKMFEFKQGVKLFGANVILSQSGYTGEDGFEIYCNIDDTEKIWDGLLEY
                     NVMPCGLGARDTLRLEAGLPLHGQDLTESITPYEGGIAFASKPLIDADFIGKSVLKDQ
                     KENGAPRRTVGLELLEKGIARTGYEVMDLDGNIIGEVTSGTQSPSSGKSIALAMIKRD
                     EFEMGRELLVQVRKRQLKAKIVKKNQIDK"
     gene            1629..2975
                     /gene="gcvPA"
                     /locus_tag="POB55_001843"
     CDS             1629..2975
                     /gene="gcvPA"
                     /locus_tag="POB55_001843"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HDI6500803.1"
                     /translation="MSHRYIPLTEKDKQEMLQTIGAKSIGELFGDVPSDILLNRDLNI
                     AEGEAETTLLRRLNRIASKNITKETHTSFLGAGVYDHYAPSVVDAMISRSEFYTAYTP
                     YQPEISQGELQAIFEFQTLICELTDMDVANSSMYDGMTSFAEACILAFSQTKKNKIVV
                     SKGLHYQALQVLHTYAKTRKEFEVVEIDLDGTVTDLKKLEAAVDDETAAVAVQYPNFY
                     GSIEDLEKIHSFIEDKKALFIVYANPLALGLLTPPGSFGADIVVGDTQPFGIPAQFGG
                     PHCGYFATTKKLMRKVPGRLVGQTQDDEGNRGFVLTLQAREQHIRRDKATSNICSNQA
                     LNALASSIAMSALGKQGIYDIAVQNIEHANYAKQQFIKKGFEVLDGTSFNEFVVKFDK
                     PIQQVNEELVKYNIIGGFDLGVVSDDFKNHMLIAVTELRTKDEIDTFVEKAGELND"
     gene            2968..4440
                     /gene="gcvPB"
                     /locus_tag="POB55_001844"
     CDS             2968..4440
                     /gene="gcvPB"
                     /locus_tag="POB55_001844"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HDI6500804.1"
                     /translation="MTSKSSPLIFERSREGRYAYSLPKSDIKTNSVESLLDDKFIRKN
                     KAEFPEVAELDLVRHYTELSNKNFGVDNGFYPLGSCTMKYNPKINEKVARIPGFSESH
                     PLQDEDQVQGSLEIIYSLQEELKEITGMDEVTLQPAAGAHGEWTALMIFKAYHENNGE
                     GHRDEVIVPDSAHGTNPASASFAGFKSVTVKSNERGEVDIDDLKRVVNENTAAIMLTN
                     PNTLGIFEKNIMEIREIVHNAGGLLYYDGANLNAIMDKVRPGDMGFDAVHLNLHKTFT
                     GPHGGGGPGSGPVGVVKELASYLPKPMVIKDGDKFKYDNDIKNSIGRVKPFYGNFGIY
                     LRAYTYIRTMGATGLKEVSEAAVLNANYIKARLSKHFEIPYKQYCKHEFVLSGVRQKE
                     FGVRTLDMAKRLLDFGVHPPTIYFPLNVEEGMMIEPTETESKETLDYFIDTLISIAEE
                     AKNDPDKVLEAPHTTVIDRLDEATAARKPILKFENLKQEK"
     gene            complement(4805..5191)
                     /locus_tag="POB55_001845"
     CDS             complement(4805..5191)
                     /locus_tag="POB55_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDI6500805.1"
                     /translation="MSASLYIAIILVIAIIAYMIVQQILNKRAVKELDQNEFHNGIRK
                     AQVIDVREKVDYDYGHINGSRNIPMTMFRQRFQGLRKDQPVYLCDANGIASYRAARIL
                     KKNGYTDIYMLKGGYKKWTGKIKSKK"
     gene            5349..6179
                     /locus_tag="POB55_001846"
     CDS             5349..6179
                     /locus_tag="POB55_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HDI6500806.1"
                     /translation="MTETWNFINTGSKDPYYNMAMDEALLNFVSRGEIDPVIRFYTWN
                     PATLSIGYFQRLQKEIDIDKVKEKGFGLVRRQTGGRGVLHDKELTYSVIVPESHPNMP
                     STVTEAYRVISQGLLEGFKNLGFNTYFAVPKTPEERQKLKQPRSSVCFDAPSWYELVV
                     EGRKIAGSAQTRQKGVILQHGSILQDIDIDELFDMFIYKNERLKLKMKEAFVEKAVAI
                     NDISDKHITISQMEEAFEKGFKKGLNIELKPLELTEAQLAEVEELTEKYRSDEWMFRK
                     "
     gene            complement(6243..6461)
                     /locus_tag="POB55_001847"
     CDS             complement(6243..6461)
                     /locus_tag="POB55_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500807.1"
                     /translation="MRNIIFYLVLIIAAIGLVMNLDAFIFSIVRMLVSFAVIAGIIYL
                     IYYFFILTEDQRKYRKAMRKYKRNQRRK"
     gene            complement(6475..7056)
                     /locus_tag="POB55_001848"
     CDS             complement(6475..7056)
                     /locus_tag="POB55_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500808.1"
                     /translation="MKKLVSIVGATLLLAGCGSQNLAPLEEKTTDLREDNHQLKLDIQ
                     ELNQQISDSKSKIKGLEKDKENSKKTASNNTKIKLMNVTSTYYDKVAKALKSYNDIEK
                     DVSKNKGDKNVQSKLNQISNDIQSAHTSYKDAIDGLSLSDDDKKTSKNIDKLNSDLNH
                     AFDDIKNGYQNKDKKQLTKGQQALSKLNLNAKS"
     gene            7161..8222
                     /locus_tag="POB55_001849"
     CDS             7161..8222
                     /locus_tag="POB55_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDI6500809.1"
                     /translation="MSRITQVHRILEQKHLDAIIILSDYNRRYLSGFTGTSGALIISK
                     DKQYLITDFRYIDQATKQAPNYEIINRKSTIIGEIKELLHQENFENVGFEGHHVSYDT
                     YLELNKSRISLISISNTVDKIRDVKDAEEIALIQKAANIVDETYEYILTVVKAGMTEK
                     ELKAILESKMLELGADGPSFDTIVASGHRGALPHGVASDKIIEKGDMITLDFGAYYNG
                     YCSDITRTFAIGEPDPKLKEIYQIVLESQMKAINEIRPGMTGAEADAISRNYLESKGY
                     GKEFGHSLGHGIGLEIHEGPMLARTIQDKLQVNNCVTVEPGVYIEGLGGIRIEDDILI
                     TENGCQVFTKCTKDLIVLT"
     gene            8248..8805
                     /gene="efp"
                     /locus_tag="POB55_001850"
     CDS             8248..8805
                     /gene="efp"
                     /locus_tag="POB55_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000626504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDI6500810.1"
                     /translation="MISVNDFKTGLTISVDNAIWKVIDFQHVKPGKGSAFVRSKLRNL
                     RTGAIQEKTFRAGEKVEPAMIENRRMQYLYADGDNHVFMDNESFEQTELSSDYLKEEL
                     NYLKEGMEVQIQTYEGETIGVELPKTVELTVTETEPGIKGDTATGATKSATVETGYTL
                     NVPLFVNEGDVLIINTGDGSYISRG"
BASE COUNT         3206 a         1223 c         1660 g         2917 t
ORIGIN      
        1 gtgaacaata tttcaggaat ttagagttca catgtttgca agaatgtatt aacactgcag
       61 atataattgc tactggtggt ggtattattg agagtgaaga ggcatttaat tttttgaaaa
      121 atcaaaaaaa cattatttgg ttagattgta atattgatat tatatatagt cgaatcaatg
      181 atgacccaca tcgacctaat gcaaataata agacaatcaa gcagttaaat gacttgtatt
      241 gctcgcggaa tttaagatat aatgaaatcg cattcaagaa atttgatagt catttgctat
      301 caatttcaga aatatattat gaattgctaa atttaataaa agcgagtgat cagtattaga
      361 gagaatagag cgttaagact ctatcgccga aggtgcaagt aatttattac gaaactctca
      421 ggcaaaagga taatactgta acgcgttcct gaattggtga tttataaaca gggtagcgat
      481 tgctatcctg tttttataat tttaaggggg tatttcaatg tcaagtgatt taaaacaaac
      541 acctttatat caaaattatg ttgatagagg tgcaaaaatt gtggaattcg gaggatgggc
      601 gatgcctgtt caattttcaa gtattaaaga ggagcataat gctgttcgat acgaaattgg
      661 cctgtttgat gttagtcata tgggtgaaat tgaagtaaca ggtaaagatg ctagtcagtt
      721 tgtgcaatat ttattatcaa atgatactga taatttaact acttcaaaag cattatatac
      781 tgctttatgt aatgaagaag gcggtattat tgatgattta gtaatatata aattagctga
      841 cgacaattat ttattagttg ttaatgctgc taatactgaa aaagatttta attggatttt
      901 aaaacacaaa gagaaatttg atgttgaagt acaaaatgta tcaaaccaat atggtcaatt
      961 agcaatacaa ggaccaaaag ctagagattt aattaatcaa ttagttgatg aagatgtaac
     1021 tgaaatgaaa atgtttgaat ttaaacaggg tgtcaaatta tttggagcaa acgtcatttt
     1081 atctcagtca ggttacacag gtgaagatgg gtttgaaatt tattgtaata tcgatgatac
     1141 tgaaaaaatt tgggatggtt tattagagta taatgttatg ccatgtgggt taggcgctcg
     1201 tgatacatta agattagagg ctggattgcc attacatgga caagatttaa ctgaatcaat
     1261 tacaccatat gaaggtggta tcgcttttgc aagtaaacca ttaattgatg ctgattttat
     1321 tggtaaatct gtattaaaag atcaaaaaga aaatggtgca ccaagaagaa cagtgggatt
     1381 agaattactt gaaaaaggaa ttgcaagaac tggttatgaa gttatggatt tagatggaaa
     1441 tattattgga gaagtaactt caggaacaca gtctccatca tcaggaaaat caattgcact
     1501 tgcaatgata aaaagagatg agtttgaaat gggtagagag ttgcttgttc aagttcgtaa
     1561 gcgtcaatta aaagcgaaaa ttgttaagaa aaatcaaatt gataaataat taaaaagggg
     1621 tgtgcattgt gagtcatcgt tatatacctt taactgaaaa agacaagcaa gaaatgttac
     1681 aaacaattgg tgcaaaatct ataggagaat tattcggtga tgtaccaagt gacattttat
     1741 taaatagaga tttaaatatt gctgaaggcg aagcggaaac aacgttactt agaagattaa
     1801 atcgcattgc aagcaagaat atcactaaag aaacgcatac atcatttttg ggtgctggtg
     1861 tttatgatca ttacgcgcca tcagttgtgg atgctatgat ttcaagatca gaattttaca
     1921 cagcatatac gccatatcaa ccggaaattt ctcaaggtga attacaagcc atttttgagt
     1981 tccaaacttt aatttgtgaa ttaacagata tggatgtagc aaattcttct atgtatgatg
     2041 ggatgactag ctttgctgaa gcatgtatct tagcatttag tcaaacaaag aaaaataaaa
     2101 ttgtagtctc aaaaggttta cattatcaag cattacaagt acttcatact tatgctaaga
     2161 cgcgtaagga attcgaagta gtggaaatcg atttagatgg cactgttaca gatttgaaaa
     2221 aattagaagc agcagtggat gatgaaacag cagctgttgc agtacaatat ccaaacttct
     2281 acggttcaat tgaagatctt gaaaagattc atagctttat tgaagataaa aaagcattat
     2341 ttatcgtata tgcaaatcca ttagcattgg gattacttac acctccaggt tcatttggtg
     2401 cagatattgt tgtaggtgat actcagccat tcggaattcc tgctcaattc ggtgggccac
     2461 attgtggata ttttgcaacg acgaaaaagt taatgcgtaa agtgccaggt agattagtcg
     2521 gtcaaacaca agatgatgaa ggtaatcgtg gctttgtatt aacattacaa gcacgtgaac
     2581 aacatattcg acgtgataaa gcgacatcta atatttgttc taaccaagca ttaaatgctc
     2641 tagcgtcatc aattgcaatg tcagcacttg gtaaacaagg tatctatgat attgcagttc
     2701 aaaatataga acatgctaac tatgctaaac agcaatttat taaaaaaggt tttgaagtat
     2761 tagatggtac atctttcaat gagtttgtag tcaaatttga caaaccaatt caacaagtta
     2821 acgaggaatt agtgaagtac aatattatcg gtggttttga tttaggtgtt gtttcagacg
     2881 actttaaaaa tcacatgtta atagctgtaa ctgaattaag aacaaaagat gaaatcgata
     2941 catttgtaga aaaggctggt gagttaaatg actagtaaat caagtccatt aatttttgaa
     3001 agatctcgtg aaggcagata tgcatattca ttaccaaaaa gtgatattaa aacaaattct
     3061 gttgagtcat tgttagatga taaatttatt cgtaaaaaca aagcggagtt tcctgaagtt
     3121 gctgaactag acttagtacg tcattatact gaactttcaa ataaaaattt cggtgtagac
     3181 aacggatttt atccattagg ttcatgtacg atgaagtata accctaaaat taatgaaaag
     3241 gttgctagaa taccaggatt tagtgaatca catccattac aagatgaaga ccaagttcaa
     3301 ggttcattag aaattattta tagtttgcaa gaagaattaa aagaaattac tggtatggat
     3361 gaggtgacat tacaaccagc tgctggcgca catggtgaat ggactgcatt gatgatattt
     3421 aaagcttatc atgagaataa tggtgaaggt catcgtgatg aagtcattgt gccagattct
     3481 gcgcatggta cgaatccagc ctcagcttca tttgcaggat ttaaatcagt tactgtaaaa
     3541 tcaaacgaac gtggcgaagt tgatattgat gacttgaaac gtgttgtaaa tgaaaataca
     3601 gcagctatta tgttaactaa tccaaacact ttaggtattt tcgaaaaaaa tattatggaa
     3661 atccgtgaaa tcgtccataa tgctgggggt ctattatatt atgatggtgc gaatttaaac
     3721 gctattatgg acaaagttcg cccaggagat atgggatttg atgctgttca tttaaacttg
     3781 cataaaacat ttactggtcc acatggtggt ggcggtcctg gttcaggtcc agtcggtgta
     3841 gtaaaagaac tagcaagtta cttaccaaag ccaatggtta ttaaagatgg cgacaaattt
     3901 aaatatgata atgacattaa aaattctatc ggacgtgtaa aaccatttta tggtaacttt
     3961 ggtatttact taagagctta tacgtatatt cgaactatgg gagcaactgg acttaaagag
     4021 gtttctgaag cagcggttct taatgcgaat tatattaaag cacgtttatc taaacacttt
     4081 gaaatacctt ataaacaata ttgtaaacac gagtttgtgt taagtggtgt gcgtcaaaaa
     4141 gaatttggtg tacgtacttt agacatggct aagcgattat tagatttcgg tgtacatcca
     4201 ccaacaatat acttcccatt aaatgttgaa gaaggtatga tgattgaacc gactgagaca
     4261 gagtctaaag aaacacttga ttattttatc gatacattaa ttagtattgc tgaagaagct
     4321 aaaaatgatc ctgataaagt gctagaagca ccacatacaa ctgtgattga tcgattagac
     4381 gaagctacag ctgctcgtaa accaatatta aagtttgaaa atcttaaaca ggaaaaataa
     4441 agtattaaac acatattccg agaattatta ttctaacttt gtatgaagat ttaaggataa
     4501 tggtttcaaa atcaattgaa aaagacaatt tctatttaaa caagaaaact aaaccgaagt
     4561 aataactctt agggtttggt attattcttt catagaaatt gtcttttcat tttttagatt
     4621 gcggtaattg aatcgtattg aaaatgagct gaactttctt tattatgctg aaactaagtt
     4681 taatgatcag tgaagctaga gaaatatagt aacagacttt gttaaaaaat taaaatctga
     4741 gtattgttat tgatgatcaa ttaacactca gattttatta aatcgtatat taaatttaca
     4801 aaaactattt tttagacttt atttttccag tccatttttt atagccgcct tttaacatat
     4861 agatatctgt atatccattc tttttcaaaa tacgagcggc tctatagcta gcaatcccat
     4921 tggcatcaca taagtatacc ggttggtctt ttcttaatcc ttggaatcgt tgcctgaaca
     4981 ttgtcatagg aatattgcga gacccattaa tgtgaccgta gtcatagtca actttctctc
     5041 taacatcgat gacttgagct tttctaatcc cattatggaa ttcattttga tctaattctt
     5101 taacagctcg cttgttaaga atttgttgaa caatcatata agcaataatt gctataacta
     5161 aaattattgc gatgtacaaa ctagcactca tcttgcatcc tccctaatta aacgataata
     5221 taattataag actgttaggt caaattatca aaatatttca ctaaagaatt acaattatta
     5281 ttcatttagt taaattgatt aattagcgaa tcaaatatta tacattttgc tgataggagt
     5341 tcttgaatat gactgaaact tggaatttta ttaatacagg aagtaaagac ccatattata
     5401 atatggcaat ggatgaagcg ttattaaatt ttgtctctag aggtgaaatt gatccagtta
     5461 tacgttttta cacatggaat cctgcaacat tatcaatagg gtatttccaa aggctacaaa
     5521 aagaaattga tattgataag gttaaagaga aaggttttgg tctcgtaagg cgtcaaactg
     5581 gtggccgtgg ggtgctacat gataaagagt taacttacag tgtgattgtg cctgaatcgc
     5641 atccgaatat gccttctaca gttacagaag cgtacagagt gatttcacaa ggattattag
     5701 aaggatttaa aaacttagga tttaatactt attttgcggt acctaaaacg cctgaagagc
     5761 gtcaaaaatt aaaacaacct cgaagttcag tgtgttttga tgcaccaagt tggtatgagc
     5821 tagttgttga aggccgtaag attgctggga gtgctcaaac gagacaaaag ggcgtaatcc
     5881 tccaacacgg ttcgatttta caagatattg atattgatga actgtttgat atgtttattt
     5941 ataaaaatga aagattaaaa ttaaaaatga aagaggcatt cgttgaaaaa gcagttgcca
     6001 tcaatgatat ttcagataaa cacattacta ttagtcaaat ggaagaagct ttcgagaaag
     6061 gttttaagaa aggtttaaat atagaattaa agccacttga attaacagaa gctcaattgg
     6121 cagaagtgga agaattaact gaaaaatatc gttcagatga atggatgttt agaaagtagt
     6181 ttttagaata aaaaatggaa gcacgtagac tattttacct acaagtgctt ccattttttt
     6241 atctattttc ttctttgatt tcttttatac ttacgcatcg ctttgcgata tttgcgttgg
     6301 tcttcagtta agatgaagaa ataataaatc agataaataa taccagctat tacagcaaag
     6361 ctgactaaca ttctgacgat tgaaaaaata aaggcatcta gattcattac taatccaatc
     6421 gcagcaataa taagtacaag ataaaatatt atattacgca ttaaaagact cctatcatga
     6481 ttttgcattt aagtttaatt ttgacaacgc ttgttgtcct tttgtaagtt gttttttatc
     6541 tttattttga tagccatttt taatatcatc aaatgcatga ttcaaatcag agtttaattt
     6601 atcgatattt ttagacgttt ttttatcatc atcactaagt gataaaccat cgatagcatc
     6661 tttgtatgaa gtgtgagcac tttgaatatc attagaaatt tgatttaatt tcgattgaac
     6721 attcttatcg cctttgtttt tacttacatc tttctcaata tcgttatagg atttcaaagc
     6781 tttagcaact ttgtcgtagt atgttgatgt aacattcatc aatttaattt tcgtattatt
     6841 agatgcagtt tttttactgt tttctttatc cttttcaagc cctttaattt tagatttaga
     6901 atcactaatt tgttgattaa gttcttgaat atctagtttg agttgatgat tatcttctct
     6961 taaatctgtt gttttttctt ctaatggtgc taaattttgt gatccacatc cagctaacaa
     7021 taatgttgcg ccaacaattg aaaccaattt tttcattact atctccctaa aatcaatatc
     7081 tattattttt tatcctcatt atgatattat tttaatgtat aaagttgaaa tgcacaaacg
     7141 tttcactagg aggtaataag atgagcagaa taacacaagt gcatcgtatt ttagaacaaa
     7201 aacatttaga tgcgattatc attctttcgg attacaatag aagatattta tcaggtttta
     7261 ctggtaccag tggagcactt atcatatcta aagataaaca atatttgata acagacttta
     7321 gatatattga tcaagcaact aagcaagcgc caaattatga aattattaat cgtaaatcta
     7381 ctattattgg tgagattaaa gaattgctac accaagaaaa ttttgaaaat gtaggttttg
     7441 aggggcacca tgtaagttat gatacatacc ttgaattaaa taaaagccgt atatcattaa
     7501 taagcatttc taatactgta gataaaatta gagacgtcaa agatgctgag gaaattgctt
     7561 taattcaaaa agcagctaat attgttgatg aaacatatga atatatttta actgttgtaa
     7621 aagcaggtat gactgaaaaa gaattaaagg caatattaga aagtaaaatg ctagaattag
     7681 gagcagatgg accatcattc gatacgattg tagcatctgg tcatagaggt gcattaccac
     7741 atggtgttgc aagtgacaaa attattgaaa aaggcgacat gattacatta gattttggcg
     7801 cgtattataa cggctattgt tcagatatta ctagaacatt tgctattgga gaaccagatc
     7861 ctaaactgaa agaaatatat caaatagtac ttgaatctca aatgaaagca attaatgaga
     7921 ttagacctgg catgactggt gcagaagctg atgccatttc aagaaactat ttagagtcaa
     7981 aagggtatgg aaaagaattt ggacattcac taggacatgg tattggttta gaaatccatg
     8041 aagggccaat gctggctcgt acgatacaag ataaacttca agttaacaac tgtgttacag
     8101 tagaacctgg tgtttatata gaaggtttgg gcggtataag aatagaagat gatattttaa
     8161 ttacagaaaa tggttgtcaa gtctttacta aatgcacaaa agaccttata gttttaacat
     8221 aagcgtgtaa aatgaggagg aaactgaatg atttcggtta atgattttaa aacaggttta
     8281 acaatttctg ttgataacgc tatttggaaa gttatagact tccaacatgt aaagcctggt
     8341 aaaggttcag cattcgttcg ttcaaaatta cgtaatttaa gaactggtgc aattcaagag
     8401 aaaacgttta gagctggtga aaaagttgaa ccagcaatga ttgaaaatcg tcgcatgcaa
     8461 tatttatatg ctgacggaga taatcatgta tttatggata atgaaagctt tgaacaaaca
     8521 gaactttcaa gtgattactt aaaagaagaa ttgaattact taaaagaagg tatggaagta
     8581 caaattcaaa catacgaagg tgaaactatc ggtgttgaat tacctaaaac tgttgaatta
     8641 acagtaactg aaacagaacc tggtattaaa ggtgatactg caactggtgc tactaaatcg
     8701 gcaactgttg aaactggtta tacattaaat gtacctttat ttgtaaacga aggtgacgtt
     8761 ttaattatca acactggtga tggaagctac atttcaagag gataatctct aatttgttaa
     8821 caaatagctt gtattcacta tactgattta acgtaagata ttctaaataa gtctcataaa
     8881 gctattgcct aaaatgatta taggttatat gctgatatga ggctttttat ttttaaaata
     8941 atttttaaaa tataaaacga atcgtctaat tggtagtgaa aaaacataga catgagtaaa
     9001 ggtata
//
LOCUS       DAMZKC010000106         8926 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.107, whole genome shotgun sequence.
ACCESSION   DAMZKC010000106 DAMZKC010000000
VERSION     DAMZKC010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8926
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.107"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(784..1890)
                     /locus_tag="POB55_001851"
     CDS             complement(784..1890)
                     /locus_tag="POB55_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HDI6500811.1"
                     /translation="MDKSLEINLKELEGIMYKEKVIDVKEYDNLKVTFETLNDAPKSE
                     IPTPFTIEQMLYKLETKNLYFPEIKSKEKCVELLHSVGYYNLKHFMYNEFGKDEPKNF
                     DEVYILYKFDRYLLKQLFSLVNMLETHIRNIILEVYMLEIEYNNKPSTLFYLDKDLYF
                     EKVNDEYKYSAKKLKEFNRLQNVFWRAIEKKKSNDNVKHNIKKYNIIPAWVLFQNFSF
                     GDLSTFYRTTLPTYRNKVSKRIENLIEKNTGISIKLPEKLLCAWLNSIRFLRNRIAHT
                     DIIYGINFTNTCAKHHSDEEMYVNIEKYKYQQRLVTFLLAMKKIFMSMPENNIIEWNE
                     TLTKIENKCSEHNFIKLSRLGVIENNLSYFKITK"
     gene            complement(2402..3943)
                     /gene="guaA"
                     /locus_tag="POB55_001852"
     CDS             complement(2402..3943)
                     /gene="guaA"
                     /locus_tag="POB55_001852"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019166941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDI6500812.1"
                     /translation="MEMAKEQELILVLDFGSQYNQLITRRIREMGVYSELHDHEISIE
                     EIKKMNPKGIILSGGPNSVYEEGSFTIDPEIYNLGIPVLGICYGMQLTTKLLGGKVER
                     ANEREYGKAIINAKSDELFAGLPAEQTVWMSHSDKVIEIPEGFEVIADSPSTDYAAIE
                     DKKRRIYGVQFHPEVRHTEYGNDLLNNFVRRVCECKGQWTMENFIEIEIEKIRQRVGD
                     RRVLCAMSGGVDSSVVAVLLHKAIGDQLTCIFVDHGLLRKGEGDMVMEQFGEGFNMNI
                     IRVNAKDRFMNKLKGVSDPEQKRKIIGNEFVYVFDDEASKLKGVDFLAQGTLYTDVIE
                     SGTKTAQTIKSHHNVGGLPEDMEFELIEPINTLFKDEVRKLGIELGIPEHLVWRQPFP
                     GPGLGIRVLGEITEDKLEIVRESDAILRQVIREEGLEREIWQYFTVLPNIQSVGVMGD
                     YRTYDHTVGIRAVTSIDGMTSDFARIDWEVLQKISSRIVNEVDHVNRVVYDITSKPPS
                     TIEWE"
     gene            complement(3968..5434)
                     /gene="guaB"
                     /locus_tag="POB55_001853"
     CDS             complement(3968..5434)
                     /gene="guaB"
                     /locus_tag="POB55_001853"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002955225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HDI6500813.1"
                     /translation="MWESKFAKESLTFDDVLLIPAQSDILPKDVDLSVQLSDKVKLNI
                     PVISAGMDTVTESKMAIAMARQGGLGVIHKNMGVEEQADEVQKVKRSENGVISNPFFL
                     TPEESVYEAEALMGKYRISGVPIVDNKEDRNLVGILTNRDLRFIEDFSIKIVDVMTQE
                     NLITAPVNTTLEEAEKILQKHKIEKLPLVKDGRLEGLITIKDIEKVIEFPNAAKDEHG
                     RLLVAAAIGISKDTDIRAQKLVEAGVDVLVIDTAHGHSKGVIDQVKHIKKTYPEITLV
                     AGNVATAEATKDLFEAGADIVKVGIGPGSICTTRVVAGVGVPQITAIYDCATEARKHG
                     KAIIADGGIKFSGDIIKALAAGGHAVMLGSLLAGTEESPGATEIFQGRQYKVYRGMGS
                     LGAMEKGSNDRYFQEDKAPKKFVPEGIEGRTAYKGALQDTIYQLMGGVRAGMGYTGSH
                     DLRELREEAQFTRMGPAGLAESHPHNIQITKESPNYSF"
     gene            complement(5472..6740)
                     /locus_tag="POB55_001854"
     CDS             complement(5472..6740)
                     /locus_tag="POB55_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HDI6500814.1"
                     /translation="MKNLILSVQHLLAMYAGAILVPIIVGTSLKFTPEQIAYLVTVDI
                     FMCGVATFLQANKVTGTGLPIVLGCTFTAVAPMILIGQTKGIDVLYGSLFLSGILVII
                     IAPFFSHLVKFFPPVVTGSVVTIIGINLMPVAMNYLAGGQGAKDYGDVKNILLGLMTL
                     IIILVLQRFTTGFIKSIAILIGLVLGTIGAGLLGMVDINQVNHAGWLGIPVPFRFSGF
                     SFDVTSTLVFFIVAIVSLIESTGVYHALSEITGKKLERKDFRKGYTAEGLAIVLGSIF
                     NSFPYTAYSQNVGLVSLSGAKKNNVIYGMVVLLLICGCIPKLGALANIIPLPVLGGAM
                     IAMFGMVMAYGVSILGHIDFKNQNNLLIIAVSVGLGTGISAVPQAFKGLGEQFAWLTQ
                     NGIVLGAISAIILNFFFNGIKYKQTEENVK"
     gene            complement(6740..7318)
                     /gene="xpt"
                     /locus_tag="POB55_001855"
     CDS             complement(6740..7318)
                     /gene="xpt"
                     /locus_tag="POB55_001855"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDI6500815.1"
                     /translation="MELLGQKVKEDGVVIDEKILKVDGFLNHQIDAKLMNEVGRTFYE
                     QFKDKGITKILTIEASGIAPAIMAALHFDVPCLFAKKAKPSTLTDGYYETSIHSFTKN
                     KTSTVIVSKEFLSEEDTVLIIDDFLANGDASLGLYDIAQQANAKTAGIGIVVEKSFQN
                     GHQRLEEAGLTVSSLCKVASLEGNKVTLVGEE"
     regulatory      complement(7549..7651)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            7831..8238
                     /locus_tag="POB55_001856"
     CDS             7831..8238
                     /locus_tag="POB55_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HDI6500816.1"
                     /translation="MADITVVNDTGELYNVINQKKSEGYLESELTIISKSKLHLNDLH
                     DSEISLISTSGTFSDRMTKLLTGEDGEHAVLSRYNLAPDELEKYKQLILDDKMLVVAV
                     RDKSSHQEVHENNSAYEEIDITHFAEASKGPKA"
     gene            8381..>8926
                     /locus_tag="POB55_001857"
     CDS             8381..>8926
                     /locus_tag="POB55_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500817.1"
                     /translation="MEFKTIQSVKDPHYNEALKLYDTKLNLSLTEDKRIIKRSLENNK
                     TENDYVFIVGLENDVVVSLATAHYEATTNSAFLIYLFAKDLPNHDELMTQTLHQIEHE
                     LNLLSNLVHSRSINFLMMEVPKEPADLTQDNGLIIEHRRQFLYEHQFERQTEIDYIHP
                     TNDGHTKKVDLFLKKNIKLTKD"
BASE COUNT         2913 a         1705 c         1271 g         3037 t
ORIGIN      
        1 ctgaagataa agaacttagt atcaatgtga ttaattccat tattattggt ttcgctaata
       61 actattaccc aatattgcaa agtttagtac cggaaataca aagtagttat ggcataacca
      121 taaataaaaa tttcaatata gatagtatag atttttaaat actatttaat aagaatagga
      181 aaccgatccc aggacataaa atccaaatcg taggctctac aatcttatat tggtagtagt
      241 tgactgcgga gccccaacat aaagaaattg gtacgccaat ttctacagat ataactccca
      301 tcatttcatc acatgatgtt gcccatacca atcgcaccaa aaaaggctag aaaccgcgta
      361 ataacggtac ttctagccta tcatttgcta tatataatta ttcccactcg atgacaagag
      421 tacttttcgt taatttgtac cagtttataa ctattgatat atcaagattt tcaatgctct
      481 atacttctca acagttcatg tcactgcata atttacgtat tcattgccta caacgtatga
      541 gatgatatat caaatcaact ctacaaggtt gtaaaatatc aacattgaaa gcaaggtaca
      601 tttactcatt atttgtggcg gataaaagat aaatgctttt tattactcct ataaaataaa
      661 ccgaccatat tataattcaa catttttata aatatagtgt tatttgaaaa tctttctaac
      721 tacaacacag ggcattattc agcataaaaa gacaatcact tagttattag tgtgcgctat
      781 tttttattta gttattttaa agtaagaaag gttattttca ataactccta acctagaaag
      841 tttaataaag ttatgttctg aacatttatt ctctatctta gttagagtct cattccattc
      901 aataatatta ttttctggca tactcataaa tattttcttc atagctaata aaaatgtaac
      961 taacctttgc tgatatttat acttttctat atttacatac atttcctcat cactatgatg
     1021 ttttgcacag gtgtttgtaa aattaattcc ataaattata tctgtgtgag ctattctatt
     1081 tctcaaaaac ctgatactat tcaaccatgc acacaataac ttttctggaa gttttatgct
     1141 tataccagta tttttttcta ttaaattttc aattcgctta cttactttat ttctataagt
     1201 tggtaatgta gttctataaa aagtagacaa atctccaaaa ctaaaatttt ggaataaaac
     1261 ccaagctggt ataatattgt attttttaat attatgttta acgttatcgt tacttttctt
     1321 tttttctata gctctccaaa aaacattttg taacctattg aactctttta gctttttggc
     1381 agaatattta tactcatcat taactttctc aaaatataaa tctttatcta aatagaacag
     1441 tgtcgaaggc ttgttattat actcaatttc taacatatat acttctaaaa taatatttct
     1501 tatatgagtt tccaacatat ttactaaact aaataattgt ttcaataaat atctatcaaa
     1561 tttataaagt atatatactt catcaaaatt tttcggctca tctttaccaa attcattata
     1621 cataaaatgc ttcaaattat aatagccaac actatgtaaa agctccacac atttttcttt
     1681 tgacttaatt tcaggaaaat ataaattttt agtttctaat ttatataaca tttgttctat
     1741 tgtaaaggga gtgggtattt cacttttagg agcatcgttt agtgtctcaa aagttacttt
     1801 taaattatca tactctttaa cgtcgattac tttttcttta tacattatgc cttctaattc
     1861 ttttaagtta atttcaagtg atttatccat aactataaat tctcctatta caaaaaaaag
     1921 gccaactgtg tttcgcattg ctaagaggcg ttagttgact tctaataaat taatattaat
     1981 ctaaggggtg tcaattgtca attgtaattc aatcattctt gtcttctcaa acctcactaa
     2041 atcatcaata aatataggtt ctaacataat cagatttacc ttatcgccaa atattctatg
     2101 ataacgacca ctttctttta tgtagtacaa aagatattta tttaaaactc ttgtctctta
     2161 gtaacaaata aattttctct cactcctcta acggatatca tctacaatca ttgtatgact
     2221 aggattgtcg ttaaatactt cttctacatt tttgtctaac ttctcatcat ctccgtctca
     2281 aaatataaga aagagatgat tcccattatt tcacgtcatg atgttgccca tgccaagcac
     2341 accaaaaagg ctggaaaccg catagtaacg gtaactccag cctatcattt gctatatata
     2401 attattccca ctcaattgtg cttggtggtt ttgatgtaat gtcatagact acgcggttga
     2461 cgtgatctac ttcgtttacg atacgactag aaatcttttg taagacttcc caatcgatgc
     2521 gtgcgaagtc acttgtcata ccgtcgatag atgttactgc acgaatacct actgtgtgat
     2581 catacgtacg gtagtctccc ataacaccta ctgattgaat gtttggtaac actgtgaagt
     2641 attgccaaat ttctctttca agaccttctt ctctaatcac ttggcgtaaa atcgcgtctg
     2701 attctctaac gatttctagt ttatcttcag taatttctcc aagtacacga ataccaagac
     2761 caggtcctgg gaatggttgt ctccacacta aatgttctgg aatacctaat tcaataccta
     2821 atttacgtac ttcatcttta aacaatgtat tgattggttc gattaattcg aattccatgt
     2881 cttctggtaa tccaccaaca ttgtgatgtg atttgattgt ttgtgctgtc ttggtacctg
     2941 attcgataac gtctgtataa agtgttcctt gcgcaaggaa gtctacacct ttcagttttg
     3001 atgcttcatc atcaaataca tatacaaatt cattaccaat gattttacgt ttttgttcag
     3061 gatctgaaac accttttaat ttattcataa aacgatcttt cgcattaaca cgaataatat
     3121 tcatgttgaa cccttcaccg aattgttcca taaccatgtc gccttcacct ttacgaagta
     3181 agccatggtc tacaaagata catgttagtt gatccccaat tgctttatgc aataatacag
     3241 caacaacaga tgaatctacg ccaccactca tcgcacataa tacacgacgg tcgcctacgc
     3301 gttgacgaat cttttcaatt tcaatttcga taaagttttc cattgtccat tgacctttac
     3361 actcacaaac acgacgtacg aagttgttta acaaatcatt accatattct gtatgacgta
     3421 cttctggatg gaattgaaca ccataaatgc gacgtttctt atcttcgatt gctgcatagt
     3481 ctgtacttgg gctatcagcg ataacttcaa agccttctgg aatttcaata actttatcag
     3541 aatgactcat ccaaacagtt tgttctgctg gtaagccagc gaataactca tctgacttcg
     3601 cattaatgat tgctttaccg tattcacgtt cattggcacg ctcaacttta ccacctagta
     3661 atttagtagt taattgcatg ccgtaacaaa taccaagtac tggaattcct aaattatata
     3721 tttccggatc aattgtaaat gaaccttctt cataaactga atttggacca cctgataaga
     3781 taataccttt tggattcatt ttcttaattt cttcaattga aatttcatga tcgtgtaatt
     3841 cactataaac gcccatttca cgaattcggc gtgtaattaa ttggttgtat tggctaccaa
     3901 agtctaagac aaggattaac tcttgttctt ttgccatttc catatttgtc gttctccttt
     3961 atcttaatta gaatgagtag ttcggtgatt ctttcgtaat ttgaatatta tgtgggtgac
     4021 tttctgctaa accagcaggg cccatacgtg tgaattgtgc ttcttcgcgt aattctctta
     4081 aatcgtgtga accagtataa cccataccag cacgcacacc gcccattaat tggtaaattg
     4141 tatcttgtaa cgcaccttta tatgccgtac gtccttcgat accttcagga acaaatttct
     4201 taggcgcttt gtcttcttgg aagtaacggt cgtttgaacc tttttccatc gcacctaaag
     4261 agcccatacc gcggtatact ttatattgtc taccttggaa aatttctgtt gcgcctgggc
     4321 tttcttcagt acctgctaat aagctaccta acataaccgc atgtccacca gcagctaatg
     4381 ctttaatgat atctcctgag aatttaatac caccatcagc aatgatagct ttaccatgtt
     4441 tgcgtgcttc agttgcacaa tcataaattg ctgtaatttg tggtacacca acacctgcta
     4501 caacacgcgt cgtacaaatt gaacctgggc caataccaac tttaacaata tctgcacccg
     4561 cttcaaataa atcttttgtt gcttctgcag ttgctacgtt accagctact aatgtgattt
     4621 ctgggtaagt cttcttaata tgtttcactt gatcgataac gcctttagag tgaccatgtg
     4681 ctgtatcgat aactaagaca tccacacctg cttcgactaa tttttgagca cgaatatcag
     4741 tgtcttttga aataccgatt gctgcggcta caagtagacg accatgttca tcttttgctg
     4801 cattagggaa ttcgattact ttttcaatat ctttaatagt aataagacct tctagacgtc
     4861 cgtctttaac taatggtaac ttttcaatct tatgtttttg gagaattttt tctgcttctt
     4921 caagtgttgt attcactgga gctgtaatta aattttcttg cgtcattaca tctacaattt
     4981 taatcgagaa gtcttcaata aaacgtaagt cacggtttgt taaaatacct actaagttgc
     5041 gatcttcttt attatcaaca attggtacac ctgaaatacg gtatttaccc attaatgctt
     5101 ctgcttcata aacgctttct tctggcgtta agaaaaatgg gtttgaaatg acaccatttt
     5161 ctgagcgttt tactttttga acttcgtccg cttgttcttc aacgcccata tttttatgaa
     5221 taacacctaa accaccttga cgagccatag caatcgccat tttagattca gttacagtat
     5281 ccataccagc agaaataact ggaatattta atttaacttt gtctgataat tgtacgctta
     5341 aatcaacgtc tttcggtaaa atatcagatt gtgctggaat taataacaca tcatcaaacg
     5401 ttaatgattc ttttgcaaat ttactttccc acattaaaaa cagcctccat ttttcaaatt
     5461 aattagttat attatttcac attttcttcc gtttgtttat actttattcc attaaaaaag
     5521 aaattaagaa taattgcaga gattgcgcct aaaacaattc cgttttgagt caaccatgca
     5581 aattgttcac ctaaaccttt aaatgcttgt ggtacagcgc ttataccagt acctaatcct
     5641 actgatacag cgataattaa taaattgttt tgatttttaa aatcgatatg acctaatata
     5701 ctaacaccat atgccattac catgccaaac atagctatca tcgcaccacc taacacaggt
     5761 agcggtatga tatttgctaa tgcaccaagc ttaggtatac aaccacatat aagtaataac
     5821 acgaccatgc cgtatataac attatttttc ttagcgccgg ataaagaaac aagtcctaca
     5881 ttttgcgaat aggctgtata cggaaatgaa ttgaatatag aacctaacac tatcgctaga
     5941 ccttccgcag tataaccttt acgaaaatct tttctttcta acttcttacc ggtaatttca
     6001 cttaacgcat gatagacacc tgtcgactca attaaactaa cgatagctac aataaagaac
     6061 actaacgtcg atgtcacatc aaagctaaat ccagagaatc taaacggcac tgggatgcct
     6121 aaccaaccgg catgattgac ttgattaata tcgaccatcc caagtaagcc agcacctatc
     6181 gttcctaaaa cgagtccaat taatatggca atactcttaa taaatccagt tgtgaatctt
     6241 tgtaaaacaa gaataatgat taatgtcatt aaacctaaca aaatgttctt aacatctcca
     6301 tagtcctttg caccttgacc tccagctaag taattcattg ctactggcat taaattgata
     6361 ccaatgatag taacaacact acccgttact actggtggga agaattttac aagatgtgaa
     6421 aagaaaggcg cgatgataat aactaatatc cctgataaaa atagcgaacc ataaagtaca
     6481 tctattcctt tcgtttgacc aattaaaatc atgggcgcaa cagccgtgaa tgtacatcca
     6541 agaacgattg gtaatcctgt tcctgttact ttattggctt gtaaaaatgt ggcaacccca
     6601 cacataaata tatctactgt aactaagtaa gcgatttgtt caggtgtaaa cttcaaactt
     6661 gtaccaacaa tgattggaac taagatggca cctgcgtaca tagctaaaag atgttgaaca
     6721 cttaggatta aatttttcat tattcttctc ccaccaatgt cactttgttt ccttctagtg
     6781 aagcaacctt gcagagagaa gaaactgtta aacctgcttc ttctaaacgt tgatgcccat
     6841 tttggaaact cttttcaaca acaataccaa taccagctgt cttagcattc gcttgctgtg
     6901 cgatatcgta taatcctaat gaagcatcac catttgctaa aaagtcatcg atgatgagta
     6961 cagtatcttc ttctgataaa aattcttttg aaacaatgac cgtacttgtt ttatttttag
     7021 taaatgaatg aatagatgtt tcataataac catccgtcaa agtgctaggt tttgcttttt
     7081 tcgcaaataa acatggcaca tcaaaatgca gtgcagccat gattgcaggt gcgataccgg
     7141 aagcttcaat ggttaagatt ttagtaatcc ctttatcttt aaattgctcg taaaaagtgc
     7201 gaccaacttc attcattaac tttgcatcaa tttgatgatt taaaaatcca tcgactttta
     7261 aaatcttctc atcaatgaca acgccgtctt cctttacttt ttgtcctagt aactccactc
     7321 ttaaaacctc ctcagatttg tgtgaaacat tttgctctta aattggtgct agatacaaaa
     7381 aaatccccaa actaaataat agtttcaggg tttatgagtg aacgaacatg cataacgaat
     7441 ttgtcatgca atcaatgtaa aagaagtttc atcaaataaa ctgtgaccat catataaaat
     7501 gatataaatc acccaccatg gttacaattt aatggctgaa gctactccta gtattgtgtt
     7561 gttactcata gtcatgtcgt tcaaggcaac atggtagaaa cttctaaagc catattcttt
     7621 agattatatg agtttatgta aattatttaa cgataatagc aaattttcgg cattttttca
     7681 ataactgctt aggtaatctt ttaatagttt tataaatgta cagagacgtt taatgcgaat
     7741 gaagacttat aacttaataa tgtaagggtt ttactttata ataagaaggg taaaagatta
     7801 aataaacaaa tttttaaagg ggcgacaact atggcagata ttactgtagt aaatgacact
     7861 ggcgaattat acaacgtaat caatcaaaag aaatctgaag gttatttaga atcagagcta
     7921 acaattatta gtaaaagtaa attacaccta aatgacttac acgactctga aatctccctt
     7981 atctcaacaa gtggaacatt tagtgataga atgactaaac tgttaacagg agaagatggt
     8041 gaacatgcag ttttatcacg ttacaactta gcgcctgatg aattagaaaa atataaacaa
     8101 cttattttag acgataaaat gttagttgta gcggttcgtg acaaatcatc acatcaagaa
     8161 gtgcatgaaa ataattcagc ttatgaagag attgatatta cacattttgc tgaagcatct
     8221 aaaggtccca aagcttagtt atcacactga attcaaaatg agattactgt actgtctaat
     8281 ttaagatagt acagtttttt ataccattta aaagtttatg aacagtcttt tacagcaggc
     8341 gattatgcta taatattatt taaattgggg aggtgttctt atggaattta aaaccattca
     8401 atctgtcaaa gatccacatt acaacgaagc acttaaactt tacgatacta agttaaattt
     8461 atctttaact gaagataaac gtatcatcaa acgctcatta gaaaacaaca aaaccgaaaa
     8521 cgattatgtc tttatcgtag gcttagaaaa cgatgttgtt gtcagtctag caactgcaca
     8581 ttatgaagcc acaaccaatt cagcattttt aatttattta ttcgcaaaag acctacccaa
     8641 ccatgatgag ttaatgacgc aaacattaca tcaaattgaa catgaactaa acttattatc
     8701 aaaccttgtt cattctagaa gtattaactt tttaatgatg gaagtcccta aagaacctgc
     8761 agatttaaca caagataatg gactcattat tgaacaccgt agacagtttt tatacgaaca
     8821 tcaatttgaa agacaaactg aaattgatta cattcatcca actaatgatg gtcacactaa
     8881 aaaagtggac ttatttttaa agaaaaatat taaactgaca aaggat
//
LOCUS       DAMZKC010000107         8861 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.108, whole genome shotgun sequence.
ACCESSION   DAMZKC010000107 DAMZKC010000000
VERSION     DAMZKC010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8861
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.108"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            16..1539
                     /locus_tag="POB55_001858"
     CDS             16..1539
                     /locus_tag="POB55_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500818.1"
                     /translation="MSKKLKIIIPIIVVLLLIGGIAWGVYAFFANTPKNTYLKSEQQT
                     AKMYKDYFNDRFENEVKFQEKMKDNSFLSSLELSADASDEIVKGLGIPKSVVNASKIK
                     MSYGHDPKKEKSMINLEPTIADSALGKFQLAADKDKHYFESPLFKGKYSVNNSDLLST
                     YSKLTGEDEETAKENGITNQQLNLNTLFSNAQAQQSDYSKIAEKYSELIVDKLDDDNF
                     DKGKKEEIKVNGEKYKVRPVTLTLSRADTKKITLAVLEEAKKDKDLKKLMEEQGATKD
                     FEKDIKKAIDDVKETKKDEFAKIQSKIYTEKHTIVKREITITDKENNKTKIKGTNTLE
                     DDKLKLDYALDFDQDKYTYAEAKYTIKGVSSKEKDNKYSDKYEFGKKTEYDESKIKLD
                     NQEKVDGTKRQDKGKITVALDKYSDENEFTFENNIDSDVKNNTQKSTLNIGIKYAEEP
                     INFILKSSTKLKADIDFDDSGAKDFNSLSSKDREKLEKEIEKNGGKMFESILKKASK"
     gene            1607..2773
                     /locus_tag="POB55_001859"
     CDS             1607..2773
                     /locus_tag="POB55_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6500819.1"
                     /translation="MKTIHLFRIYHSFLLKKWYLIIYLLFILAALVITLTTIQHVTED
                     DNHFNIGVVDKDQSSETKLILNSIGKGSNLGKNVSIKAYDDKQAHTLLKKHKLQGYFV
                     FDKGMTKAFYKQGELPISVYTYDQQSMKSVVLSQLTDSVYQRLMRSMGGILAFQDLAP
                     KASHSDSINVMTDLLITGLNRSGAFNLEPIHLYDTGSYYAITGFLTTVFIFALSLFTV
                     LKMNQDTVLKARLKMFHFSKERLLIIRVLITWFYTMLWSIVGVVWIVFSIPNTFELYN
                     WPTLAIHLSYYVTFLILWLLLIELLTTGLLNSISKVILAIVILVLSGLTIPTIFLQHI
                     ANGVFNIQPFAVVTNQLLEIILNNYILELHPSFYLSFIALLIINLAVLVWRYRQ"
     gene            2770..3690
                     /locus_tag="POB55_001860"
     CDS             2770..3690
                     /locus_tag="POB55_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6500820.1"
                     /translation="MKPYIQLVVFKQWLQYILLVTTIVIALVLIGIGYRVAHDNFKIP
                     ITIQDLDQTTASKSFVNKIKQSDYVTIKKVDEDESYIEDDVTKKEAILSMQIPKGFSQ
                     KLKENRLKETIQLYGRDDFIGGIAVEIVSSSLYEQQIPNIIYEHLEDMKQHQSIDAIN
                     KSYHKHTPESKIKFVSLTKQAQQSISISLIFAVILFVSAVQVVLHYRLNQQAALQRLS
                     QYHLTRFKLYSTYVMTHTILLLLVLLAVSLYLSQPLSLIFYLKSLLLILIYEIGIVFI
                     LFHIQTISHRLFMTFIYALAMGIVYLIIFM"
     gene            3704..4363
                     /locus_tag="POB55_001861"
     CDS             3704..4363
                     /locus_tag="POB55_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6500821.1"
                     /translation="MIEINNLSKRYRNKQIFNHLTMSFDSNRLTVLLGDNGAGKSTLL
                     RMIAGIEKANDGTINYFGEKWNQRQIQNHIGYVPQDIALFEHMTVAENINFFKSLCKN
                     PINDTTINEYLQQLNFDDTSAKVSTLSGGNKRKINILVGLLGQPRILILDEPTVGIDL
                     KSRHDIHQLLNIMKSKCLIILTTHHLDEVEALADDIKLIGQDPFYQHVLEDKQWAYTY
                     Y"
     gene            complement(4415..5365)
                     /gene="lytM"
                     /locus_tag="POB55_001862"
     CDS             complement(4415..5365)
                     /gene="lytM"
                     /locus_tag="POB55_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-glycine endopeptidase LytM"
                     /protein_id="HDI6500822.1"
                     /translation="MKKLTAAAIATMGFATFTMAHQADAAETTNTQQAHTQMSTQSQD
                     VSYGTYYTIDSNGDYHHTPDGNWNQAMFDNKEYSYTFVDAQGHTHYFYNCYPKNANAN
                     GSGQTYVNPAIAGDNNDYTASQSQQHINQYGYQSNVGPDASYYSHSNNNQAYNSHDGN
                     GKVNYPNGTSNQNGGLASKATASGHAKDASWLTSRKQLQPYGQYHGGGAHYGVDYAMP
                     ENSPVYSLTDGTVVQAGWSNYGGGNQVTIKEANSNNYQWYMHNNRLTVSAGDKVKAGD
                     QIAYSGSTGNSTAPHVHFQRMSGGIGNQYAVDPTSYLQSR"
     gene            complement(5691..6683)
                     /locus_tag="POB55_001863"
     CDS             complement(5691..6683)
                     /locus_tag="POB55_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HDI6500823.1"
                     /translation="MCTGFTIQTLNNQVLLGRTMDYDYPLDGSPAVTPRNYRWTSRTG
                     TTGQTQYGFIGTGTDMEGFIYGDGVNEHGVAISTQYFRGYSSYGSTHKAGAMNITQNE
                     IVTWILGYTTSIEDMKQQASQIHVVAVYLNDIGEVPPLHYHVSDATGHSVEVSFKEGE
                     VVIKDNPIGVLTNHPDLDWHYSNLRQYINISPYPATAKLLEGVTIEPLGNEAGTFGLP
                     GGFTSTERFVRMAFMKANIAQNNDKEMDLMNAFYLLDAVNIPIGIVRPHDADNHYTMY
                     QTVINLTTRTLYIKYYGSNELVALKLTDDLINRKDMTIFKPEKHITIRKLNDNQ"
     gene            6917..8293
                     /locus_tag="POB55_001864"
     CDS             6917..8293
                     /locus_tag="POB55_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6500824.1"
                     /translation="MNNTQSSPRSNIIIAIMLSALTYWLFAQSFINIGPLVGQTYQTS
                     PAVLNLSISLTSFATGIFMVAAGDIADKIGQLRMTYMGLIISMFASLLLIISDITALL
                     IIGRILQGLSAAILLPSTVGVLNNQFKGEHLRRAISYLMISTVGGIGLAGVIGGLIAT
                     NFGWQTNFIISIVIAFIAILLLKGTPEKVSQHSHRHPFDYKGMSIFAVMIGSFTLLLT
                     QGFEQGWFSTFSFICLSIFIITTLIFIIIERRHEVPFIDFSVLRNRPFIGAFLNNFVL
                     NSGLGVTVVFFIYAQTHLGLSAAQSGLVTLPYAIVAVAMIRLGEKATLRFGGKLMLII
                     GPLFPVIGITIISMTQLSASQYVIAVIIGFVICAIGNGLVATPGLTIAIFSMPNEKVG
                     LATGLYKMSGTLGGAFGIALSTTVFSMLQLNYAPSVAATVTFIVSIVLMILGSLSAYM
                     IIPKTVKS"
     gene            complement(8421..8813)
                     /locus_tag="POB55_001865"
     CDS             complement(8421..8813)
                     /locus_tag="POB55_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500825.1"
                     /translation="MQFKLKEEEIISFLELKYPEKEFEYGRLLVGQHKRDDLDVYYFG
                     DTFLMCTIISFKTFEIKETVELSYDAVNRIVLKDGWLFRKMRIETMQKVLKYGTSKLM
                     LTDFQKENYNKYIQGQKQRVIFENGHFV"
BASE COUNT         2956 a         1525 c         1403 g         2977 t
ORIGIN      
        1 ttgggaggaa acaaaatgtc taaaaaacta aaaattataa ttcctattat tgttgtctta
       61 ttattaatag gtggaatcgc atggggagtt tatgcatttt ttgctaatac accgaaaaat
      121 acatacctaa aaagtgaaca acaaacagca aaaatgtata aagattattt taatgaccgt
      181 tttgaaaatg aagtgaagtt ccaagaaaag atgaaagata attcattttt atcttcatta
      241 gaattaagcg cagatgcatc agatgaaatt gttaaggggc ttggtattcc taaatctgtt
      301 gttaatgctt cgaaaattaa aatgtcatat ggacatgatc ctaaaaaaga gaaatcaatg
      361 attaatcttg aaccaacaat agcagactct gcattaggga aattccagtt agctgcagat
      421 aaagataagc attatttcga atcaccatta tttaaaggga aatatagtgt taataattct
      481 gatttattat caacttattc aaaacttaca ggtgaagatg aagaaacagc aaaagaaaat
      541 ggtattacaa accaacaact aaatttaaat actcttttta gtaatgctca agcacaacaa
      601 agtgactaca gcaaaattgc cgaaaaatat tccgaactta ttgtcgacaa attagatgac
      661 gataattttg ataaaggtaa aaaagaagaa attaaggtta atggtgaaaa gtacaaagtt
      721 agacctgtca cgttaacact tagcagagct gacactaaaa aaattacatt agctgtatta
      781 gaagaagcta aaaaggataa agaccttaaa aaattaatgg aagaacaagg tgctacaaaa
      841 gactttgaaa aagacatcaa aaaagcaatt gacgatgtca aagaaactaa aaaggatgaa
      901 tttgctaaaa ttcaatctaa aatttatacc gaaaaacata cgattgtaaa acgagaaatt
      961 actattacag acaaagaaaa taataaaact aaaatcaaag gtactaatac tttagaagac
     1021 gataagttaa aactagatta cgcacttgat ttcgatcaag ataaatacac gtatgctgaa
     1081 gcgaaatata caattaaagg cgtatcttct aaggaaaaag acaataaata cagtgataaa
     1141 tacgaatttg gtaaaaagac agaatatgat gaatcaaaaa tcaaattaga taaccaagaa
     1201 aaagtagatg gcacaaaacg tcaagataaa ggtaaaatta ctgtcgcatt agataaatat
     1261 agcgacgaaa atgaattcac ttttgaaaat aatatagatt ctgacgtcaa aaataacact
     1321 cagaaatcta cgttaaatat cggcatcaaa tatgctgaag aaccaattaa tttcatttta
     1381 aaatctagca caaaattgaa agcagatatt gattttgatg atagtggtgc gaaagatttc
     1441 aatagtctat cttcaaaaga ccgtgaaaaa cttgaaaaag aaatcgaaaa aaatggcggc
     1501 aaaatgtttg aatcaatttt aaaaaaggca tctaaataac taaaatataa aaataagatt
     1561 ggaaggtcat cttgcctttc aatcttatca ttggaggagt ggttaaatga aaacaataca
     1621 tttgtttcgc atctaccact cttttttatt gaaaaagtgg tatttgatta tctatttact
     1681 atttatatta gcggcactcg ttattacatt aacgacgatc caacatgtaa cagaagatga
     1741 caatcatttt aatataggtg tcgtagataa agatcaatca agtgaaacga aattaatctt
     1801 aaactctatt ggtaaaggga gtaacctagg aaaaaacgtg agcattaagg catatgatga
     1861 taagcaagca catactttgt taaaaaaaca taaacttcaa ggctattttg tttttgataa
     1921 aggtatgacc aaggcatttt ataaacaagg cgaactacca atttcagtat atacatatga
     1981 tcaacaatcc atgaaaagtg tcgtgctatc tcagctaaca gattctgttt accaacgtct
     2041 tatgcgatca atgggtggca tcttagcttt tcaagactta gcaccgaaag catcacattc
     2101 tgacagtatc aatgttatga ctgatttgct gattacagga ttaaaccgtt caggtgcatt
     2161 taacttagaa ccgattcatt tatacgatac gggcagttat tatgcaatta caggtttttt
     2221 aacaacggta ttcatctttg cactatcttt atttacagtt ttgaaaatga atcaagatac
     2281 tgtattgaaa gcgcgattga aaatgtttca tttttctaaa gagcgtttat taatcattcg
     2341 tgtgttgatt acatggtttt atactatgtt atggagtatc gttggtgtag tttggattgt
     2401 gttcagtatt ccgaatacct ttgaattata taattggcca acgttagcca ttcatttaag
     2461 ctattatgtt acctttttaa tactgtggtt attactcatc gaacttttaa caacaggctt
     2521 gttgaatagt attagtaaag tcatattagc tattgtcatc ctggtgttat ctggtttaac
     2581 aatacctacg atttttttac aacatatagc aaatggcgtt ttcaatattc aaccgttcgc
     2641 agttgtaaca aatcaattgt tagaaattat tttaaacaac tacattttag aactgcaccc
     2701 tagtttctat cttagtttca tcgcactttt gataattaat ttagctgtat tggtttggag
     2761 gtatcgtcaa tgaagcctta tatacaactt gttgtgttca agcaatggtt acaatacatc
     2821 ttgctcgtaa caaccattgt catcgcactc gtacttattg gtatcggtta ccgtgtagca
     2881 catgacaact tcaaaatacc gattaccatt caagatttag accaaaccac tgcatcaaaa
     2941 tcattcgtca ataaaattaa acaatctgac tatgtaacta ttaaaaaagt cgatgaagat
     3001 gaaagctata ttgaagatga tgttactaaa aaggaagcta ttttaagtat gcaaattcct
     3061 aaaggtttct ctcaaaaatt aaaagagaac cgtttaaaag aaacgataca gttatatggt
     3121 agagatgact ttataggtgg tattgctgta gaaattgtta gtagttcatt atatgaacag
     3181 caaattccta acattattta tgaacacctt gaggatatga aacaacatca atccatcgat
     3241 gctatcaaca agtcctatca taaacataca cctgaatcta aaatcaaatt tgtgtcgctt
     3301 actaaacaag cacaacaatc tatttcaatt agcttaatct ttgctgtgat tttatttgtt
     3361 agcgctgttc aagtagtact tcattatcgt ttaaaccaac aagcagcatt gcaacgatta
     3421 tcacaatatc atttaacccg tttcaaacta tatagtactt atgtaatgac acatacgatc
     3481 ttgttattgt tggtactatt ggcagttagt ctatatttgt ctcaaccact cagcttaata
     3541 ttttacttaa aatcactgtt acttatattg atttatgaga taggtatcgt ctttatctta
     3601 ttccatattc aaacaataag tcatcgatta ttcatgacat ttatatatgc acttgctatg
     3661 ggtatcgtat acttgattat tttcatgtaa aggagcgtaa ctgatgatag aaattaataa
     3721 cctttcaaag cgttaccgta acaaacagat tttcaatcat ttaaccatgt cctttgatag
     3781 taatcgttta accgtattac ttggtgataa tggcgctgga aaatcaacat tacttcgtat
     3841 gatcgctggt attgaaaaag ctaatgatgg aactatcaac tatttcggcg aaaaatggaa
     3901 tcaaagacaa atccaaaatc acatcggtta tgtgccacaa gacattgcgt tatttgaaca
     3961 catgacagtg gctgaaaaca ttaatttttt taaatcactt tgtaaaaatc caattaacga
     4021 tacaactatc aacgaatatt tacagcaatt aaattttgat gatacgtctg ccaaagtatc
     4081 tacattgtcc ggtgggaata aacgtaaaat taatatatta gtaggtttac taggtcaacc
     4141 tcgaattcta attttagatg aaccgacagt tggtattgat ttgaaatcta gacatgacat
     4201 ccaccaactg cttaacatta tgaaatctaa atgtttaatt atattaacta cccatcattt
     4261 agatgaagtt gaagcacttg cagatgatat caagttaatt ggccaagatc ctttttatca
     4321 acatgtttta gaggacaagc aatgggctta tacctattat taaaacgaaa aaatcccaag
     4381 ttgcgtatga tatcgtaact tgggatttac tgtattatct actttgcaag tatgacgttg
     4441 ggtctactgc atattgatta ccgatgccac cagacatacg ttggaagtgt acgtgaggcg
     4501 ctgttgaatt acccgtacta cctgaatatg caatttggtc accagctttg actttatcac
     4561 cagctgaaac agttaaacga ttattatgca tataccattg gtagttatta ctgttcgctt
     4621 ctttaatagt tacttgattg ccgccaccat agttactcca acctgcttgt actactgtgc
     4681 catcagttaa tgagtaaact ggtgaatttt caggcattgc atagtcgaca ccgtaatgcg
     4741 caccaccacc gtgatattgt ccatatggtt gtagttgttt acgacttgtt aaccagcttg
     4801 cgtctttcgc atgaccacta gctgtcgctt tacttgctaa tccgccattt tgattcgatg
     4861 tgccattagg gtaattgacc tttccattac catcatggct gttatacgct tggttgttgt
     4921 tactatgtga ataatagctc gcgtctggac ctacatttga ttgataacca tattgattaa
     4981 tatgctgttg gctttgactc gctgtgtagt cattgttatc tcctgctatt gctggattca
     5041 catatgtttg gccgcttcca ttggcatttg cattttttgg ataacagtta taaaaataat
     5101 gcgtatgtcc ttgagcatct acgaatgtat agctatattc tttattatca aacattgctt
     5161 gattccagtt accatcaggt gtgtgatgat aatccccatt agaatcaatt gtataataag
     5221 taccataaga tacgtcttgt gattgtgttg acatttgtgt atgtgcttgt tgggtgtttg
     5281 tcgtttctgc tgcatctgct tgatgcgcca ttgtaaatgt agcgaagccc atcgttgcaa
     5341 tcgctgctgc tgttaatttt ttcatgtata aaacatcctc cattaaaatt aaagttagtt
     5401 ttcaattaaa ctgtactgca catactaaaa gaattagaca actgagtaaa ggatttaatt
     5461 ctcattttcc aactatttaa tattcccgaa atgttttact aaactcatta cattgtcatt
     5521 acaaaatagc catacattga tattaaaatg acatctctca ctgcattcgt ttaacctttt
     5581 ataaattttc aaaactaaca actaatcgtt cgtccatgtt cgcgattcaa cgctaatgta
     5641 tgatagtaat catccatcaa atcatatcaa ccaaattcca ttatcaatcg ctattgattg
     5701 tcattcaact ttctaatagt gatatgcttc tcaggcttaa aaatcgtcat atcttttcta
     5761 ttaattaaat catctgtgag ctttaatgct actaattcat tgctgccata atacttaata
     5821 tataacgttc ttgtagttaa atttattacg gtctgataca tcgtatagtg attgtcagca
     5881 tcatgcggac gtacaattcc aatcggtata tttaccgcat ctaataaata aaatgcattc
     5941 attaaatcca tttctttatc attgttttga gcaatgtttg ctttcataaa tgccattctc
     6001 acaaagcgct cagttgaagt aaatccacct ggcaatccaa atgtacctgc ttcattgcct
     6061 aaaggttcaa tcgttacacc ttccaataac tttgctgttg ctggataagg agaaatattg
     6121 atatattgtc ttaaattact ataatgccaa tctaagtctg gatgatttgt taagacacca
     6181 atagggttat cttttataac cacttcaccc tctttaaatg aaacttcgac tgaatgtcca
     6241 gttgcatcgg aaacatgata atgcaatggc ggaacttcac cgatgtcatt taaatatacc
     6301 gctacaacat gtatttggga tgcttgttgt ttcatatctt caatgcttgt tgtatatccc
     6361 aaaatccatg tcacaatttc attttgcgta atattcatcg cgcccgcttt gtgtgttgat
     6421 ccatatgaac tataacctcg gaaatattgt gttgaaatgg caacgccatg ttcattaaca
     6481 ccatcaccat aaataaaacc ttccatatct gttcctgtac caataaagcc atattgcgtt
     6541 tggcctgtcg tgccagtgcg agatgtccaa cgataatttc taggcgtcac tgctggcgaa
     6601 ccatctaatg gataatcata atccatcgtg cgtccaagaa gtacttgatt atttaaagtt
     6661 tgtattgtga atcctgtgca cattgttctc actcctctgt accttcattt actttaatca
     6721 ctttcaaata aagctgtttc acttaaacat actataaaaa ttcaatcata caagcaatta
     6781 attgatattc attctcaata actgtggtat gatatgtaag gaaatcatga ctaatgtgtg
     6841 agtgaacgat catctataca tccgttcact tcatctcatg actttctata tttaattttt
     6901 acaaggagtg acatctgtga ataacacaca atcttcacca cgcagtaata ttattattgc
     6961 gattatgttg tctgcattaa catattggtt gtttgcacaa tcatttatta atataggacc
     7021 tctcgttggt caaacgtatc aaacctctcc tgccgtgtta aatttatcta ttagtttaac
     7081 ttccttcgcc acaggtatct tcatggtggc tgcaggtgat attgctgata aaataggaca
     7141 actgagaatg acatacatgg gtctcataat cagtatgttt gcatctcttc tattaattat
     7201 atcggacatc actgcactgc tcatcatcgg tagaatttta caaggtctat cagcagctat
     7261 cttgttacct tcaacagttg gcgttttaaa taatcaattt aaaggagaac atttaagacg
     7321 agcgattagt tatctaatga ttagtactgt tgggggcatc ggcctagctg gtgttatcgg
     7381 cggtttaatt gccacaaatt tcggatggca aacgaatttc atcattagta tagtcattgc
     7441 tttcattgcc atattacttc taaaaggcac acctgaaaaa gtaagtcaac atagccatcg
     7501 tcatccattc gattacaaag gtatgtcgat tttcgctgtt atgattggta gctttacatt
     7561 attgttaaca caaggattcg aacaaggttg gtttagtaca ttttcattca tttgtctgag
     7621 catttttatc atcactacgt tgatattcat catcatcgaa cgtcgacatg aagtaccttt
     7681 tattgatttc tcagtattac gcaaccgtcc gttcattggt gcatttttaa ataactttgt
     7741 tttaaatagt ggcctaggcg taacagtggt ctttttcata tatgctcaaa cacaccttgg
     7801 tttatcagct gcgcaatctg gacttgttac attgccatat gccattgtgg cagttgcgat
     7861 gattcgttta ggtgaaaaag caacattacg tttcggtggc aaattgatgc tcatcattgg
     7921 tccgttgttc cctgtcatcg gcatcactat tattagcatg actcaattat cggcatcaca
     7981 atatgtcatt gcagttatca ttggtttcgt catttgtgca attggaaatg gtttagtcgc
     8041 aacacccggc cttacaattg caattttcag tatgccaaat gaaaaagttg gtttagctac
     8101 aggattatat aaaatgagtg gtacattagg tggcgccttt ggtatagcac taagtactac
     8161 agttttcagt atgttacaac taaactatgc accaagtgta gctgcaaccg taacatttat
     8221 cgtcagcatt gtattgatga tccttggctc attgtctgca tacatgatca ttccaaaaac
     8281 ggttaaatct taaatataat agaagcatca tgtttcgaaa tatctgtatc actttaaaat
     8341 gatatacaag aaattcaaga aaaataagcg aagaacgttt ttctctgttc ttcgcttact
     8401 ctaattatat tcactatcaa ttagacaaaa tggccatttt caaatatcac gcgttgtttc
     8461 tgaccttgaa tatatttatt ataattctct ttttgaaaat cagttaacat taatttagat
     8521 gtaccgtatt ttaacacttt ttgcattgtt tctattctca tttttctaaa taaccatcca
     8581 tcttttaaca caatacgatt aacagcatca tatgataatt ctactgtttc tttaatttca
     8641 aatgtcttga atgaaataat cgtgcacatt aaaaacgtat caccaaagta ataaacatct
     8701 aaatcatcac gtttatgttg tccaactaac aaacgaccat attcgaactc tttttctgga
     8761 tatttcaatt ctaaaaaact aataatctct tcttctttta atttgaattg catttaaaaa
     8821 catcctctct tatgttttaa caagccttaa ttaaaaattt t
//
LOCUS       DAMZKC010000108         8816 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.109, whole genome shotgun sequence.
ACCESSION   DAMZKC010000108 DAMZKC010000000
VERSION     DAMZKC010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8816
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.109"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(<1..277)
                     /locus_tag="POB55_001866"
     CDS             complement(<1..277)
                     /locus_tag="POB55_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDI6500826.1"
                     /translation="MKHFAQLTLPIETAVRIPQNDISRYVNEIVKKIADSEFDEFRHH
                     RGATSYHLKMMLKITSYLYTQSEFSGRRIEKLLHNSIRMMWLAQDQTP"
     gene            complement(474..1166)
                     /locus_tag="POB55_001867"
     CDS             complement(474..1166)
                     /locus_tag="POB55_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HDI6500827.1"
                     /translation="MNKAERQNLIITAIQQNKKMTALELAKYCNVSKRTILRDIDDLE
                     NQGVKIYAHYGKNGGYQIQQAQSKIALNLSETQLSALFLVLNESQSYSTLPYKSEINA
                     IIKQCLSLPQTRLRKLLKRMDFYIKFDDTQHMTLPMLFSDILIYCTERNVMLVDHRVD
                     DNIKAENVIFIGLLCKHGHWHAVIYDIAQDKTAELEIENIIDISYSFGKTIQTRDISI
                     DNYHQFLNPIDS"
     gene            1551..2348
                     /locus_tag="POB55_001868"
     CDS             1551..2348
                     /locus_tag="POB55_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HDI6500828.1"
                     /translation="MTDKTLQQIDKLICSWLKQIDNVIPQLIMEMTTETKRHRFDLVT
                     NVDKQIQQQFQQFLATYFPEHQLLAEEKSNAMITNEINHLWIMDPIDGTANLVKQQED
                     YCIILAYFYEGKPMLSYVYDYPHKKLYKAIRGEGAFCNGIKMEEPPSLKLEDAIISFN
                     AQVMNLDTVQDLFDASFSYRLVGACGLDSMRVAKGQFGAHINTNPKPWDIAAQFLFAE
                     LLNLKMTTLDGKAIDHLKGAPFIISNKACHETVLKILNANGGYQKYR"
     gene            2481..3428
                     /locus_tag="POB55_001869"
     CDS             2481..3428
                     /locus_tag="POB55_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDI6500829.1"
                     /translation="MKRSSKSKMSLPKKIFLWVFGILVILAIVAVVYVAAKIFITGNK
                     IHNPLDRNHSELRDKKVSLNDGDPFTIALFGVDSDADRKKKGGGERSDSIMILSINPK
                     TKKTEIVSIPRDTRAEIVGRGTTEKIAHAYAYGGPNMAVKSLEKLMNVPIDHYATIDM
                     DGLHNMIDSIGGVDVVSNDTFTVDGVRFTKGQQTHVNGDQALKFIRSRKEEGAGGDFG
                     RQQRQQIVLEAMANKIASPSSITHFNSLMNEIQNNVKTDLTLGDLNTIRSNYKDANDT
                     INKHQLSGQGGIQSDGLYYFIPSEQSKAESTKLLKDNLE"
     gene            4030..6984
                     /gene="fdhF"
                     /locus_tag="POB55_001870"
     CDS             4030..6984
                     /gene="fdhF"
                     /locus_tag="POB55_001870"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HDI6500830.1"
                     /translation="MQEHLVVTLDGKDYLVEPGTNLLEFIKSQDTFVPSICYNESMGP
                     IQTCDTCTVEIDGKIERSCSTVIDRPMTVNTVNNDVKDAQKEALDRILEKHMLYCTVC
                     DYNNGDCEIHNTMDAWGLQHQTYEYKEKPYEKDYGPFYRYDPNQCILCGRCVEACQDI
                     EVNETIRIDWDREHPRVIWDNDVPINESSCVSCGQCATVCPCNAMMEVNMEGNAGYMT
                     DTEPGSLAAMIDLTKKAEPGYGPLFAISDSEAEMRKERIKKTKTVCTYCGVGCSFEVW
                     TKDREILKVQPSHDSPANKIATCVKGKFSWGHINSDQRLTKPLVRKNGEFHEVEWDEA
                     LNVIADNFTSIKEKYGPDALSFISSSKATNEESYLMQKLARQVIGTNNVDNCSRYCQA
                     PATKGLFRTVGHGGDSGSIEDLEKAAMSVLIGTNTAEAHPVIASRMKRAQKLFGQKIH
                     VFDIRKHEMAERADRFYQPKPGTDLAWLSAVTKYIIDHDLHDKAFIDEWVDDFDEYYK
                     SLETFTMAFAEEATGIPESELIKFAEECAKAESVVICWAMGITQQDIGSDSSTAISNL
                     LLVTGNYRRPGTGAYPLRGHNNVQGCSDMGSMPDKITGYQSIEADDIRAKFEKEYGVK
                     LNPKAGKDNHEMVEGIHDGEVHSLYLYGEDTGIVDSNINFVQAAFEKLDFMVVQDEFL
                     TFTATYADVVLPASPSLEKDGTFTNTERRIQRLYQALEPLGDSKPDWKIFQAIANRLG
                     FDWNYKHPSEIMDEVARLTPLYAGVSYDRLEGFNSLQWPVQPDGTDEPILYLEGFNFD
                     NGKAKLFPLSFDNYFKQDEIYDIHVNNGRLLEHFHEGNMTYQTPMIKYKVPRAFVEIS
                     PELAEDRGIHEGAEVKLISETGEAVLQVHVTDRVKGKEIYIPLNNDAMENGDLGAINL
                     LTNSDVDQYTDTPSYKRTSCRLEVITKRGKSPLNPNNFRVNKKRHPQYSVQVQKKWER
                     SDYVFPGNQVDK"
     gene            6984..7457
                     /locus_tag="POB55_001871"
     CDS             6984..7457
                     /locus_tag="POB55_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1641 domain-containing protein"
                     /protein_id="HDI6500831.1"
                     /translation="MAERISSKIRRLEKSEEQIKLESLNEVTEAIAANKDSILKAIKL
                     IKTLDDAKLLDALNGAIRGRQVIINKFAVELNKDIYTGLLSNMASMVFLLGELNVSDL
                     SDFLNKVNKGLHVANQASPNAKTTIRSLLGVLKDDDMNRSLTYMLNMLKGMSREE"
     gene            7685..7918
                     /locus_tag="POB55_001872"
     CDS             7685..7918
                     /locus_tag="POB55_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500832.1"
                     /translation="MLKTSAVISNTLLVIGLVCLFMMKLVLAITFFAVSLSISLVVFN
                     MMFRDRTTMKVIVNASFLIVILAIVVAYFLLSK"
     gene            7940..8716
                     /locus_tag="POB55_001873"
     CDS             7940..8716
                     /locus_tag="POB55_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HDI6500833.1"
                     /translation="MKKNFKLRISTLLLIVILVVFAVLLIVNETKLFKNDVNYSFDEA
                     VSMQQGKGIVQTKEEDGKFVEANNNEIAKAMTISHKDNDMKYMDITEKVPMSESEVNQ
                     LLKGKGILENRGKVFLEAQEKYEVNVIYLVSHALVETGNGKSELAKGIKDGKKRYYNF
                     FGIGAFDSSAVRSGKSYAEKEQWTSPDKAIIGGAKFIRNEYFENNQLNLYQMRWNPEN
                     PAQHQYASDIRWADKIAKLMDKSYKQFGIKKDDIRQTYYK"
BASE COUNT         3159 a         1164 c         1716 g         2777 t
ORIGIN      
        1 aaggtgtttg atcttgagct aaccacatca ttcgaatact gttatgaagt aatttttcta
       61 ttctacggcc agaaaattca gattgagtat ataaatatga ggtgattttt aacatcattt
      121 ttagatgata ggatgttgcg ccacgatgat gtctgaattc atcgaattcg ctatcagcta
      181 tctttttaac aatttcatta acatatcgcg aaatatcatt ttgaggaatt ctaacagcag
      241 tttctattgg tagtgtaagt tgggcaaagt gttttatttt tttaagtatt tcaaagtaaa
      301 attacatgtt aatacgtagt attaatggcg agactcctga gggagcagtg ccagtcgaag
      361 accaaggctg agacggcacc ctaggaaagc gaagccattc aatacgaagt attgtataaa
      421 tagagaacag cagcaagata ttttctaatt gaaaatcatc ttactgctgt tttttaggaa
      481 tcgatggggt ttaaaaattg atgatagtta tcaatggata tgtctctggt ttgaatcgtc
      541 ttaccgaatg aatacgaaat atctataata ttttcaattt caagttcggc agttttgtct
      601 tgagcaatgt cataaatgac tgcatgccaa tgtccatgtt tacacaaaag gccaataaat
      661 ataacgtttt cagctttaat attatcatca accctatgat ctactaacat cacatttcgt
      721 tctgtacaat aaattaaaat gtcggaaaac agcattggga gtgtcatatg ttgtgtgtca
      781 tcaaatttaa tataaaagtc catgcgttta agcaattttc ttaagcgtgt ttgtggaaga
      841 cttaaacatt gttttataat tgcgttgatt tcgcttttat atggtaatgt cgagtacgac
      901 tgactttcat taagcactaa aaataaggcc gataattgtg tttcagataa gtttaatgca
      961 attttagatt gtgcttgttg tatttggtaa ccaccatttt tcccataatg cgcataaatt
     1021 ttaacacctt gattttctaa atcatcaata tctcttaaaa ttgtgcgttt ggatacgttg
     1081 caatatttag ctaattctaa agcggtcatt tttttatttt gttgaattgc agtaattatt
     1141 aaattttgcc tttcagcttt attcatgaca gccacctcct aaaagatgat atattacaca
     1201 aagtataaca taagagtgac aatgaggaca ttttcaatat cttttaaaaa aaatcgtaaa
     1261 aattttgtgg aaaacgctta cattttaaaa aagattgtgc tatgatatta cttgtaacag
     1321 ggggagcgat taaacaaagg ggtagagcta tataacagat agcttttaat cgttcaagtt
     1381 acatcacaac atcttcaata tttattactt actttccttt ctatttgtcg gctagcacgt
     1441 gactagccga tttttttatg cataaaatga agaagtcttc agttgtattg ttgattcttt
     1501 taatataggg taataataaa atgataatga tatgagaagt ggtggacgaa atgacagata
     1561 aaacattaca gcaaatagat aagttaatat gttcgtggtt gaaacaaata gacaatgtta
     1621 ttccgcaatt aattatggaa atgactacag aaactaagcg tcatcgattt gatttagtta
     1681 caaatgtaga taaacagatt caacaacaat ttcaacaatt tttagcaaca tattttccgg
     1741 aacaccaact attagcggaa gaaaaaagta atgcaatgat tacgaatgaa attaatcatt
     1801 tatggattat ggatcccatt gatggaactg ccaatttagt aaaacagcaa gaagattatt
     1861 gtattatatt ggcgtatttt tatgaaggta aaccaatgtt atcgtacgta tatgattatc
     1921 cgcataaaaa gctttataaa gcaatacgag gagaaggtgc tttttgtaat ggaattaaga
     1981 tggaagaacc accatcgtta aaattggaag acgctattat atcgtttaat gcacaagtga
     2041 tgaatctaga tacggtgcaa gatttatttg acgcctcatt tagttatcgt ttagttggtg
     2101 cttgcggatt agattctatg cgagtggcaa aaggtcaatt tggggcacat attaatacaa
     2161 atcctaaacc ttgggacatt gctgcacagt ttttatttgc agaactattg aatctaaaaa
     2221 tgacgacttt agatggtaag gcaattgatc atttaaaggg tgcacctttt attataagta
     2281 ataaagcatg tcatgaaacg gtacttaaaa ttttaaatgc aaatggtggt tatcaaaaat
     2341 acagatagat attctctaga gataaaaaat gataaaaaat agttatacgt ctacagaagt
     2401 atagtaaaaa aattatttta ctatataata aatagagaaa acagacaaag tatgtaagga
     2461 atagtaaagg agtggtggga ttgaaacgtt cgtctaaaag taaaatgagc ctaccgaaaa
     2521 aaatattttt atgggtattc ggtattttag taatcttagc gattgtagct gttgtgtatg
     2581 tagctgctaa aatttttatt actggtaata agatacacaa tccgttagat cgcaatcatt
     2641 cagaattaag agataaaaaa gtgagtttaa acgatggtga tccattcaca atcgctttat
     2701 ttggtgtcga ttcagatgct gatcgtaaga aaaagggtgg cggagagcgt agtgatagta
     2761 ttatgatttt atctatcaac cctaaaacga agaaaactga aatagtaagt ataccgcgtg
     2821 atacaagagc ggaaattgtt ggccgtggta ctactgaaaa aattgcacat gcctatgcat
     2881 atggtggacc aaatatggct gttaaatcac ttgaaaaatt aatgaatgtg ccaattgatc
     2941 attatgcgac aatcgatatg gatggtttgc ataatatgat agatagcatc ggcggtgttg
     3001 atgtagtaag taatgataca ttcactgttg atggtgttcg ttttacaaaa ggacagcaga
     3061 cacatgtaaa tggtgatcaa gcattgaagt ttattagaag tcgtaaagaa gaaggtgctg
     3121 gtggtgactt tggtcgacaa caacgtcagc aaattgtact tgaagcgatg gctaataaga
     3181 ttgcaagtcc atcatcgatt acacatttta atagtctaat gaatgaaatt caaaataatg
     3241 ttaaaacaga tttaacatta ggtgatttga atacgattag aagcaattat aaagatgcta
     3301 atgacacgat taataaacat caattgagtg gtcaaggcgg cattcaaagt gacggattgt
     3361 actacttcat tcctagtgag cagtcaaaag ctgaaagtac taagttatta aaagataatc
     3421 tagagtagta aactaaggga aaataagtta tcaaaattgt acacaaatta attagataga
     3481 atgatctgta aatagaataa cgaatagaac taatagcccg taaatagaag gtgataacac
     3541 tttgtattta tgggctattt tatttaatgc ctgatttgat ttttataaat taatccatta
     3601 caaatttaat ttaaagataa atcttacctt ctaaatgata gtagatattt atcaaacttg
     3661 ggttattaat tttcagaaaa agggtattta taaatagtat aacgattttt aggtgaggtg
     3721 attattaaat attgtcatta ataaggttgt gaaagatatg gcaaaaagcg tgaaacagat
     3781 aattggaaaa ctaataatga taattatttt caattaaaca aaaagatgat ttaatttcgc
     3841 gcaaaatatt tcaaataaaa atgattgtaa aaaggcaaaa tacaaaattt cacttaacaa
     3901 ctagtacata aagtaataca attaaattaa ttctatctga aagatgtgtg gggcatcgtt
     3961 attttaggtg gatatgagca atttattaaa agtcatttac ggaaaatata tatagacggg
     4021 gtgagtaata tgcaagaaca tttggtggtt acacttgatg gaaaagatta tcttgtagaa
     4081 ccaggtacga atttacttga atttattaaa tcacaagata cttttgtacc ttcaatttgt
     4141 tataacgagt cgatgggacc aattcaaaca tgtgatacat gtactgttga aattgacggt
     4201 aaaattgaac gttcatgtag tacggtgatt gatcgtccaa tgactgtaaa tactgtgaac
     4261 aatgatgtga aagatgctca aaaagaagcg cttgatcgaa ttttagaaaa gcatatgctg
     4321 tattgtacag tatgtgatta taataatggt gattgtgaaa ttcataatac gatggatgca
     4381 tgggggcttc agcatcaaac gtatgaatat aaagagaagc catacgaaaa agattacggc
     4441 ccattttatc gttatgatcc aaatcaatgt atcttatgtg ggcgttgtgt agaagcatgt
     4501 caagatatcg aagtgaatga aactattaga attgattggg atcgtgaaca tccacgtgtt
     4561 atttgggata atgatgtacc gattaatgag tcttcatgtg tatcttgtgg tcaatgtgcg
     4621 acggtatgtc catgtaatgc catgatggaa gtgaatatgg aaggtaatgc gggttatatg
     4681 actgatacgg aacctggttc attagcagca atgattgatt taactaaaaa agcagaacct
     4741 ggttatggtc cactatttgc aatctcagat tcagaagcag aaatgcgtaa agagcgtatt
     4801 aagaaaacta aaacagtgtg tacttattgt ggtgtgggtt gttcatttga agtttggact
     4861 aaagatagag aaattttaaa agtacaacca tcacatgatt ctccagcaaa taaaattgcg
     4921 acttgtgtta aaggtaagtt ttcatgggga catattaatt cagatcaacg attaactaaa
     4981 ccactagtaa ggaaaaatgg tgagttccat gaagtagaat gggatgaagc tttaaatgtc
     5041 attgcagata attttacatc tattaaagaa aagtatggcc cagatgcact atcattcatt
     5101 tcttcttcta aagcgacgaa tgaagaatcg tatttaatgc aaaaattagc aagacaagtt
     5161 attggaacaa ataacgttga taactgttca agatattgcc aagcacctgc aacaaaaggc
     5221 ttatttagaa cggttggaca cggcggtgac tcaggtagta ttgaagatct agaaaaagcg
     5281 gcaatgtctg tattgatagg taccaataca gccgaagcac acccagttat tgcatcacgc
     5341 atgaaacgtg ctcaaaaatt atttggacaa aaaatacatg tgtttgatat tagaaaacat
     5401 gaaatggctg aacgtgcgga tcgtttttat caaccgaaac caggtacgga tttagcgtgg
     5461 ttaagcgcag tgactaagta tattattgat catgatttac acgataaagc atttattgat
     5521 gagtgggtag atgattttga tgaatattac aaatcattag aaacatttac aatggctttt
     5581 gctgaagaag caacaggtat tcctgaatca gaattgatta aatttgctga agaatgtgct
     5641 aaagctgaat ctgttgtaat ttgttgggca atgggtatta cacaacaaga cattggtagt
     5701 gactcaagta cagcgatttc aaacttatta ttagtaacag gtaattatcg tcgtcctggt
     5761 actggtgcgt atccattacg tggacataat aatgttcaag gatgtagtga tatgggaagt
     5821 atgcctgata agattactgg ttatcaaagt attgaagcgg atgatattcg cgctaaattt
     5881 gaaaaagaat atggcgttaa attgaatcca aaagctggta aagataatca tgaaatggta
     5941 gaaggtatac atgacggaga agtacactca ttgtacttat atggtgaaga tacgggtatt
     6001 gtagattcaa atattaattt tgtacaagct gcgtttgaaa aattagattt catggtagtc
     6061 caagatgaat ttttaacatt cacagcaaca tacgcagatg ttgtattgcc agcaagtcct
     6121 tcacttgaaa aagacggtac ttttacaaat accgaacgtc gtattcaacg tttataccaa
     6181 gcattagaac ctcttggtga ttcaaaacct gactggaaaa ttttccaagc aattgctaat
     6241 agattaggat ttgattggaa ttacaagcat cctagtgaaa ttatggatga agtagcacgc
     6301 ttaacacctc tatatgctgg ggtaagttat gatcgtttag aaggattcaa tagtttacaa
     6361 tggccagtac aacctgatgg tactgatgag cctattctat acttagaagg attcaatttt
     6421 gataatggta aagcaaaatt attcccatta tcatttgata attactttaa gcaagatgaa
     6481 atttatgata ttcatgtaaa caatggtcga ttgttagagc acttccatga aggtaatatg
     6541 acttatcaaa caccaatgat taaatacaaa gtgccacgtg catttgttga aatttctcca
     6601 gagcttgctg aagatagagg cattcatgaa ggtgcagaag ttaagctgat ttctgaaaca
     6661 ggagaagcgg tattacaagt tcacgtgaca gatcgtgtta aaggtaaaga aatctatatt
     6721 ccattaaata atgatgcgat ggaaaatgga gatctaggtg caattaactt attaactaat
     6781 agtgatgttg atcaatacac ggatacacca tcttataaac gtactagctg tcgtttggag
     6841 gtaattacga aacgtggtaa gtcaccgttg aaccctaata atttccgtgt caataaaaaa
     6901 cgtcatccgc agtacagtgt tcaagtacag aaaaaatggg aacgttcaga ttatgttttc
     6961 ccgggaaatc aggtggataa ataatggctg agagaatatc ttctaaaatt agacgcttag
     7021 aaaaatcaga agaacaaatt aagttagaaa gtttaaatga agttacagag gcaattgcag
     7081 cgaataaaga tagtatatta aaagcaatca agcttattaa aacattagat gatgcaaaac
     7141 tattagatgc tttaaatggt gcaattcgag gacgtcaagt catcataaat aaatttgcag
     7201 ttgaacttaa taaagatatt tatacagggc tattatctaa tatggcttca atggtatttt
     7261 tattaggtga actcaatgta tcagatttaa gcgacttcct aaataaagtt aataaaggat
     7321 tacacgttgc caatcaagcg agtccaaatg ctaagacaac tataagaagt ctattgggcg
     7381 tattgaaaga tgacgacatg aaccgtagtt taacatacat gcttaatatg ctaaaaggta
     7441 tgtctcgaga agaataacat tacaatgaaa aagtgtttct acatgcaagc agtgtaggag
     7501 cacttttttt aatttttgta aaaattaaaa agggtgtttt tgatggaata aatgaataat
     7561 attgaaaaac aaaaaaatca cgtgctattg tgaatcaatc atcgacaggc ctatgaaaca
     7621 tcatgtatta ttatgattat gtaagttatt taaataaaaa gggtgagtgc atattaattc
     7681 gagaatgtta aaaacgtcag ctgtaatttc taatacatta ttagtcatag gtttagtctg
     7741 tctatttatg atgaaattag tgttagctat tacttttttt gctgtttcat tatcaattag
     7801 tctagttgtc tttaatatga tgttcagaga tagaacaacg atgaaagtaa tcgttaacgc
     7861 ttctttttta atcgtaatat tagcgattgt agtggcatat tttttattgt ctaagtaaga
     7921 aaaatgaggt tttgtctata tgaagaagaa tttcaagtta cgcatttcaa cgctactatt
     7981 gatagttatt ttagttgttt ttgctgtatt actcatcgtg aatgaaacta aattgtttaa
     8041 aaatgatgtg aattactctt ttgatgaggc tgtttcaatg caacaaggga aaggtattgt
     8101 acagacaaaa gaagaggatg gtaaatttgt tgaagcaaat aataatgaaa ttgctaaagc
     8161 aatgactatt tcacataaag acaatgatat gaagtatatg gatatcacag aaaaagtgcc
     8221 aatgtcggaa tctgaagtta accaattgct aaaaggtaag gggattttag aaaatcgagg
     8281 gaaagttttt ctagaagctc aagaaaaata tgaggttaat gtcatttatc ttgttagcca
     8341 tgcattagta gaaacaggta acggcaaatc agaattagca aaaggcatta aagatggaaa
     8401 aaaacgctat tacaactttt ttggtatagg agcattcgat agtagtgctg ttcgtagtgg
     8461 gaaaagttat gctgaaaagg aacaatggac atcaccagat aaggcgatta ttggtggtgc
     8521 aaagttcatt cgtaatgaat attttgaaaa caatcaactg aatttatatc aaatgcgatg
     8581 gaatccagaa aatcctgcgc aacatcaata tgcgagtgac attcgctggg cagataaaat
     8641 tgccaaatta atggataaat cctataagca gtttggtata aagaaagatg atattagaca
     8701 aacatattat aaataagaca tcggtgttta aaggagctgg aacaatttat tgtttcgagc
     8761 tcctttagca cattctgagt gtgttagtta aatggatttt aacctaacaa aaaacg
//
LOCUS       DAMZKC010000109         8815 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.110, whole genome shotgun sequence.
ACCESSION   DAMZKC010000109 DAMZKC010000000
VERSION     DAMZKC010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8815
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.110"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            174..935
                     /gene="sufC"
                     /locus_tag="POB55_001874"
     CDS             174..935
                     /gene="sufC"
                     /locus_tag="POB55_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HDI6500834.1"
                     /translation="MASTLEIKDLHVSIEDKEILKGVNLTINTDEIHAIMGPNGTGKS
                     TLSSAIMGHPSYEVTKGEVLLDGVNILELEVDERAKAGLFLAMQYPSEITGVTNADFM
                     RSAINAKREEGQEINLMQFIKKLDKNMDFLDIDKDMAQRYLNEGFSGGEKKRNEILQL
                     MMLEPKFAILDEIDSGLDIDALKVVSKGINQMRGENFGALMITHYQRLLNYITPDKVH
                     VMYAGKVVKSGGPELAKRLEEEGYEWVKEEFGSAE"
     gene            1033..2340
                     /gene="sufD"
                     /locus_tag="POB55_001875"
     CDS             1033..2340
                     /gene="sufD"
                     /locus_tag="POB55_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HDI6500835.1"
                     /translation="MTTDILNISEEQLVDYSKAHNEPSWMTELRKKALKLTETLEMPK
                     PDKTKLRKWDFDSFKQHDVKGDVYQSLSQLPESVREIIDVDHSKNLVIQHNNTIAYTQ
                     VDDNASKDGVIVEGLADALMNHSDLVQKYFMKDAVTVDEHRITALHTALVNGGVFVYV
                     PKNVVVEHPVQYVVLHDDENASFYNHVIIVTEESAEVTYVENYLSNASGEGNQLNIIS
                     EVIAGANSNITYGSVDYMDKGFTGHIIRRGITEADASINWALGLMNEGSQIIDNTTNL
                     FGDRSTSSLKSVVIGTGEQKINLTSKIVQYGKETDGYILKHGVMKEHASSVFNGIGYI
                     KHGGTKSIANQESRVLMLSEHARGDANPILLIDEDDVQAGHAASVGRVDPDQLYYLMS
                     RGISQREAERLVIHGFLDPVVRELPIEDVKRQLREVIERKVSK"
     gene            2446..3696
                     /locus_tag="POB55_001876"
     CDS             2446..3696
                     /locus_tag="POB55_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDI6500836.1"
                     /translation="MNEVAEHSFDVNEVIKDFPILDQKVNGKRLAYLDSTATSQTPVQ
                     VLNVLEDYYKRYNSNVHRGVHTLGSLATDGYENARETVRRFINAKYFEEIIFTRGTTA
                     SINLVAHSYGDANVEEGDEIVVTEMEHHANIVPWQQLAKRKNATLKFIPMTADGELNI
                     EDIKQTINDKTKIVAIAHISNVLGTINDVKTIAEIAHQHGAIISVDGAQAAPHMKLDM
                     QEMNADFYSFSGHKMLGPTGIGVLFGKRELLQKMEPIEFGGDMIDFVSKYDATWADLP
                     TKFEAGTPLIAQAIGLAEAIRYLERIGFDAIHKYEQELTIYAYEQMSAIEGIEIYGPP
                     KDRRAGVITFNLQDVHPHDVATAVDTEGVAVRAGHHCAQPLMKWLNVSSTARASFYIY
                     NTKEDVDQLINALKQTKEFFSYEF"
     gene            3686..4150
                     /locus_tag="POB55_001877"
     CDS             3686..4150
                     /locus_tag="POB55_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HDI6500837.1"
                     /translation="MNFNNLDQLYRSVIMDHYKNPRNKGVLDNGSMTVDMNNPTCGDR
                     IRLTFDIEDGIIKDAKFEGEGCSISMASASMMTQAVKGHSLGEAMQMSQEFTKMMLGE
                     DYVITEEMGDIEALQGVSQFPARIKCATLAWKALEKGTVAKEGKAEGTTEEE"
     gene            4301..5698
                     /gene="sufB"
                     /locus_tag="POB55_001878"
     CDS             4301..5698
                     /gene="sufB"
                     /locus_tag="POB55_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019469760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HDI6500838.1"
                     /translation="MAKKAPDVGDYKYGFHDDDVSIFRSERGLTENIVREISNMKNEP
                     EWMLDFRLKSLKLFYKMPMPQWGGDLSELNFDDITYYVKPSEQAERSWDEVPEEIKRT
                     FDKLGIPEAEQKYLAGVSAQYESEVVYHNMEKELEEKGIIFKDTDSALQENEELFKKY
                     FASVVPAADNKFAALNSAVWSGGSFIYVPKNIKLDTPLQAYFRINSENMGQFERTLII
                     ADEGASVHYVEGCTAPVYTTSSLHSAVVEIIVHKDAHVRYTTIQNWANNVYNLVTKRT
                     FVYENGNMEWVDGNLGSKLTMKYPNCVLLGEGAKGSTLSIAFAGKGQVQDAGAKMIHK
                     APNTSSTIVSKSISKNGGKVIYRGIVHFGRKAKGARSNIECDTLILDNESTSDTIPYN
                     EVFNDQISLEHEAKVSKVSEEQLFYLMSRGISEEEATEMIVMGFIEPFTKELPMEYAV
                     EMNRLIKFEMEGSIG"
     gene            complement(5766..6815)
                     /locus_tag="POB55_001879"
     CDS             complement(5766..6815)
                     /locus_tag="POB55_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDI6500839.1"
                     /translation="MPVYKNDNTGKWYFSIRYKDVYGNNKRKMQRGFSTKREAKRAEA
                     IFLNDVNEGYSDSKTFDYVFHHYLENSDLRPKTKRRKQNEYHKHFKAKFGHIKMNKIT
                     QNQCQEFRKYLIENVASTNSARTIWSGFKVVINYAKKYFGLRTDPTISIKPIPRVKPK
                     PKFMMREEFEERIKDIEEQDYRELFTLMFYTGLRIGEAMALVWTDYNKYKKEISINKT
                     MDISNRTIYPRPKTDSSEDIVPLPKFINTMLTERHQREKELNKYFDERCYFIFGGMAP
                     KHYSHVQKKFQKAFPHYNIHALRHSYASYLANNGVDIFVLQSLMRHAQITETMGTYSH
                     LYTQKKHDAIAIFDK"
     gene            6928..7107
                     /locus_tag="POB55_001880"
     CDS             6928..7107
                     /locus_tag="POB55_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000337827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HDI6500840.1"
                     /translation="MCVKFTDAEIAYIKESVENYSSEFDIYDDEQELKLKIYEQIMLK
                     IESEYKDIYLFRLIN"
     gene            complement(7087..8019)
                     /locus_tag="POB55_001881"
     CDS             complement(7087..8019)
                     /locus_tag="POB55_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500841.1"
                     /translation="MEENKTLKEYLRNFLEGYKYVVENRYIYQFSSNPEAFPFMRKDD
                     YKISIFYLNQSFFEEPCIVVISNDSKLKEIYNFRNTDIKHLSKHFTSYIYDSKKYVEE
                     QSGLLDFNNYIYYTSIYYGKYIGTVIIQNNLDLFFNYGKRLANDHYNTLISKSKEKLI
                     NKAHDEIQPFNHLDLNSMKKIVDDITFSYQIEQGLQAYKRELYLPAAATFAVAIETFL
                     IKLKKVNKIKHKDTDSTMYTKLLGELTKEGKVNYRTKKRVEIAYSMRNIINHSQAGAV
                     AKGDCDFLLNTLKDIVDENERILTEYSKSINKTE"
     gene            complement(8051..8776)
                     /locus_tag="POB55_001882"
     CDS             complement(8051..8776)
                     /locus_tag="POB55_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015977653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HDI6500842.1"
                     /translation="MKELPKSRLTFKESMIESQYLATKTKEEKKQYKQLSVEDKREIL
                     KEYQSKPRKEVKFESEINKSDENLSKIYQRFSEIGVEDLFGTKKEVKELPMILKDNEN
                     IMYVTSGLYNNNTYLIVCTDLRLLFLDKGMIYGLKFHEFPFEKINSVSYKKGLLFGEI
                     IIHHGSSSIAIGSISKNTVSRMAETIQEQISIRESSMKPSNSEKMSFSVADELIKYKE
                     LLDVGVISQEEFDKKKQQLLDID"
BASE COUNT         2977 a         1307 c         1576 g         2955 t
ORIGIN      
        1 tagagttgta ctttagagtt attaaaaata ataaaaaggg tttaagttgt ttatattaag
       61 gtataagtaa gttataatta actgaacgca ttattacaaa gtctttttga ctacaaatta
      121 aaattattat aaactagtta agaaaacttt atattttacg gagggaatat aaaatggcat
      181 caacattaga aatcaaagac ctacatgtgt ctattgagga taaagaaatc ttaaaaggtg
      241 ttaacttgac aattaacact gatgaaatac atgcgattat gggaccaaac gggacaggta
      301 aatcaacttt atcatctgca attatgggac acccaagcta tgaagtaact aaaggagaag
      361 tacttttaga cggtgtaaat attttagaat tagaagttga tgaaagagca aaagcaggat
      421 tattcttggc aatgcaatat ccatcagaaa ttacaggtgt tacaaatgct gatttcatgc
      481 gttcagcaat caatgcgaaa cgtgaagaag gacaagaaat caacttaatg caatttatta
      541 agaaattaga taaaaacatg gattttctag acatagataa agacatggca caacgttatt
      601 taaatgaagg tttctcaggt ggagagaaga aacgtaacga aatcttacaa ttaatgatgt
      661 tagaacctaa gtttgcaatc ttagatgaaa tcgattcagg gttagacatc gatgcattaa
      721 aagttgtatc taaaggtatt aaccaaatgc gtggggaaaa ctttggtgca ttaatgatta
      781 cacactatca acgattatta aattacatta ctcctgataa agtacatgta atgtatgctg
      841 gcaaagtcgt taaatctggt ggtccagaat tagcaaaacg tcttgaagaa gaaggatatg
      901 aatgggttaa agaagagttc ggttcagctg aataatctta ttaatacagt atccatgaga
      961 tgttcatcta tatatgatga aaatgaacat ttatacgaaa tagtaaattt catcaagtag
     1021 gaggaaaaag ttatgacaac tgatattttg aacatttctg aagaacaact tgttgattat
     1081 tctaaagccc acaatgaacc ttcttggatg acagaattac gtaaaaaagc tttgaaatta
     1141 acagaaactt tagaaatgcc aaaacctgat aaaacaaaat taagaaaatg ggattttgat
     1201 tcttttaaac aacacgatgt aaaaggtgat gtttatcaat ctttatcaca attacctgag
     1261 tcagtaagag aaattattga cgtagatcat tctaaaaact tagtaattca acataataat
     1321 acgattgcgt acacacaagt tgatgataat gcatcgaaag atggcgttat cgttgaaggt
     1381 ttagcagacg ctcttatgaa ccatagtgat ttagtacaaa agtactttat gaaagatgca
     1441 gtaacagtag atgaacatcg tatcacagcg ctacacacgg cattagttaa tggtggcgta
     1501 tttgtttatg ttcctaaaaa tgtagttgta gaacatccag tacaatacgt tgtgttgcac
     1561 gacgacgaaa atgcaagctt ttataaccat gttatcatcg ttactgaaga aagcgccgaa
     1621 gtcacatatg ttgaaaatta cttatcaaat gcatctggtg aaggaaatca attaaatatt
     1681 atttctgaag tgattgctgg tgcaaattca aatatcacat atggctcagt ggactatatg
     1741 gataaaggct ttacaggtca tatcattcga cgtggtatta ctgaagcgga tgcctcaatt
     1801 aattgggcac taggtttaat gaatgagggt agccaaatta ttgataatac aacaaattta
     1861 tttggtgatc gttcaacaag ttcacttaaa tcagtagtta taggtacagg cgaacaaaaa
     1921 attaatctaa catctaaaat cgtacaatat ggtaaagaaa cagatggtta tatccttaaa
     1981 catggtgtta tgaaagaaca tgcatcgtct gtatttaatg gtatcggcta cattaagcat
     2041 ggtggaacta aatcaattgc taatcaggaa tcacgtgtat taatgttatc tgaacatgct
     2101 cgtggtgacg cgaatcctat tttattaatt gatgaagatg atgtacaagc tggtcatgct
     2161 gcatcagtag gtcgtgttga tccagatcaa ctttactatt taatgagtcg tggtatttct
     2221 caaagagaag cggaacgtct tgttatacat ggtttcttag atccagtagt acgtgaatta
     2281 cctatcgaag acgttaaacg tcaattgaga gaagtaattg aacgcaaagt ttctaaataa
     2341 tattttgaaa ataaaagttt gtaatagata tagactgtcg atattggtat aagactaata
     2401 caacgtcagt atttaaatga ttaggatttt tatttaagaa aggtcgtgaa tgaagtggcc
     2461 gaacactcat ttgacgttaa tgaagtaatc aaggattttc cgatattaga tcaaaaagtc
     2521 aatggcaaac gtttagcata tcttgattca acagcgacaa gtcaaacgcc tgtgcaagtg
     2581 ttaaatgttt tagaagatta ctacaagcgt tataattcaa acgttcatcg tggtgttcat
     2641 acattaggat cattggcaac tgatggttat gaaaatgccc gtgaaaccgt tcgtcgtttt
     2701 attaatgcga agtattttga agaaatcatt tttacacgcg gaacaactgc gtcgattaac
     2761 cttgtagcac atagctatgg tgatgcaaat gttgaagagg gcgatgaaat tgttgtcact
     2821 gaaatggaac atcatgccaa tattgttcct tggcaacagt tagcaaagcg taaaaatgcg
     2881 acattgaaat ttataccaat gacagctgac ggtgaattaa acatcgagga tattaagcaa
     2941 acgattaatg ataaaacaaa gatcgttgct attgcacata tatctaatgt gctcggtaca
     3001 attaatgatg ttaaaaccat tgcagaaata gctcatcaac atggtgcaat tatcagtgtt
     3061 gatggggcgc aagcagcacc acatatgaaa cttgatatgc aagaaatgaa tgctgatttt
     3121 tatagtttta gtggtcataa aatgcttgga ccaacaggta ttggcgtatt atttggtaaa
     3181 cgtgagttac tacaaaaaat ggaaccgatt gagttcggtg gcgacatgat tgattttgta
     3241 agtaagtatg atgcaacatg ggctgattta cctactaaat ttgaggcggg tactccatta
     3301 attgctcaag caattgggct tgcagaagct attcgctatt tagaacgcat aggttttgat
     3361 gcaattcata aatatgaaca agaattaacg atatatgctt atgagcaaat gtctgcaatt
     3421 gaaggaattg aaatttatgg cccgccaaag gatcgtcgtg caggtgtaat aacgtttaat
     3481 ttacaagatg tacatccaca cgatgttgct acagccgtag atacagaagg tgtagcggtt
     3541 agagctgggc atcattgtgc gcaaccgtta atgaaatggt taaatgtgtc ttcaacagct
     3601 agagcgagtt tttatatata caacacgaaa gaagacgttg atcagttaat aaatgccttg
     3661 aaacaaacga aggagttttt ctcttatgaa ttttaataat ctagatcaat tatatagatc
     3721 tgtcattatg gatcattata aaaatcctag aaataaaggt gtattagata acgggtctat
     3781 gacagtagat atgaataacc cgacatgcgg tgaccgtata cgactaacat ttgatataga
     3841 agacggcatt ataaaagatg ctaagtttga aggtgaaggt tgttcgattt caatggcaag
     3901 tgcatcgatg atgacacaag ctgttaaagg tcattcactt ggagaagcaa tgcaaatgag
     3961 ccaagaattt acgaaaatga tgcttggtga agactatgtg attacagaag aaatgggaga
     4021 tattgaagca ttgcaaggtg tatctcaatt cccagctcgt attaaatgtg ccacattagc
     4081 ttggaaagca ttggaaaaag gtactgttgc taaagaaggt aaagcagaag gtacgactga
     4141 agaagaatag aatgctgtta atcatagata attttgatat tagacatata aaagtataaa
     4201 aatttttata agatgtcatg tcattgttat aatatggttt acatcatgaa ttaaaaactt
     4261 acgcacgccg ttgtaaatat atttttaagg agtgattgaa atggctaaaa aagcacctga
     4321 tgttggggat tataaatatg gattccacga cgatgatgtg tccattttca gatcagaacg
     4381 tggtttaact gagaatatcg ttagagaaat ttctaacatg aaaaatgagc cggaatggat
     4441 gttagatttc cgtcttaaat cattaaaatt gttttataaa atgccaatgc ctcaatgggg
     4501 tggcgactta tcagaattga atttcgatga cattacttac tatgtaaagc cttcagaaca
     4561 agctgaacgt tcatgggatg aagtgccaga agaaattaaa agaactttcg ataaattagg
     4621 aattcctgaa gctgaacaaa aatatttagc tggtgtttct gctcaatatg aatctgaagt
     4681 tgtttaccat aatatggaaa aagaacttga agaaaaaggt attatcttta aagatacaga
     4741 tagtgcttta caagaaaatg aagaattatt caaaaaatac tttgcttctg tagtacctgc
     4801 agcagataac aaatttgcgg cgttaaactc agcagtatgg tcaggtggtt cgttcattta
     4861 tgtacctaaa aatatcaaac tagatacgcc actacaagct tatttccgta ttaactctga
     4921 gaacatgggt caatttgaac gtacattaat cattgctgat gaaggtgctt ctgtacatta
     4981 cgtagaaggt tgtactgcac cagtttatac aactagttct ttacactctg ctgttgtgga
     5041 aatcattgtg cataaagatg cgcacgttcg ttatactacg attcaaaact gggcgaacaa
     5101 tgtatacaat ttagttacaa aacgtacttt tgtttatgaa aacggaaata tggaatgggt
     5161 agatggtaac ttaggttcta agttaacgat gaaatatcca aactgtgttc ttttaggtga
     5221 aggtgcaaaa ggtagtacat tatctattgc atttgctggt aaaggacaag ttcaagatgc
     5281 cggtgctaaa atgattcata aagcacctaa tacatcttct acaattgttt ctaaatctat
     5341 ttctaaaaat ggtggtaaag ttatttatcg cggtattgtt cattttggac gtaaagcaaa
     5401 aggtgctcgt tcaaatattg aatgtgatac attaatctta gataacgaat caacatcaga
     5461 tacaattcca tataacgaag tattcaacga tcaaatatca ttagaacatg aagccaaggt
     5521 ttcaaaagtt tctgaagaac aattattcta tctaatgagt cgtggtattt ctgaagaaga
     5581 agcgacagaa atgattgtta tgggattcat cgaaccattt acaaaagaac ttccaatgga
     5641 atacgcggtc gaaatgaacc gtttaatcaa gttcgaaatg gaaggtagta ttgggtagct
     5701 ttaaaccgcg ttgttaagcc attcttgact tccggaaatg gctattgata ccattttgat
     5761 accatttact tgtcaaaaat ggctattgca tcgtgttttt tctgagtata taaatggctg
     5821 taagtgccca tcgtttcagt gatttgagca tgtctcatga gtgactgtaa aacgaaaata
     5881 tctacaccat tatttgcaag ataagatgca taagaatgtc ttaacgcgtg aatgttataa
     5941 tgggggaaag ctttttgaaa tttcttttga acatgactgt aatgtttggg agccattcct
     6001 ccgaaaataa aataacaacg ttcatcaaaa tatttgttta actctttttc acgttgatgt
     6061 cgttcagtta acattgtatt gatgaattta ggtaaaggaa caatatcctc tgaactatct
     6121 gtttttggtc tcggatatat agttctatta gagatgtcca ttgttttatt tatggatatc
     6181 tcttttttgt atttattgta gtctgtccaa acaagagcca tagcttcgcc aatccttaaa
     6241 cctgtataaa acattaatgt aaataactct ctgtaatctt gctcttcaat gtctttgatt
     6301 ctttcttcaa attcttcacg catcataaac ttaggttttg gctttacacg cggaataggt
     6361 ttaattgata ttgttggatc tgtacgtaat ccaaagtatt ttttagcata attaattaca
     6421 actttaaaac ctgaccaaat tgtacgagca gaatttgttg atgctacatt ctctattaga
     6481 tatttacgaa actcttggca ttgattttgt gttatcttat tcatttttat gtgcccgaac
     6541 ttagctttaa agtgtttatg atattcattt tgtttgcgtc gttttgtttt aggtctcaaa
     6601 tcgctatttt ctaaatagtg atgaaaaaca taatcaaatg ttttcgaatc actatatcct
     6661 tcgtttacgt cattcaaaaa aatagcctct gctctcttag cttcacgctt agttgaaaaa
     6721 ccgcgttgca tcttacgttt gttattaccg tatacatctt tatatctaat ggaaaaatac
     6781 catttacctg tattatcatt cttatatact ggcattttgc ttctccctcc tcaaaattgg
     6841 caaaaaaata ataagggtag gcgagctacc cgaaatttag tactaggtac taaatgtgat
     6901 ataataaaat aaaaagtagg tgatgaaatg tgcgtaaagt ttactgacgc agaaatagct
     6961 tatataaaag aatcagttga aaattatagt agtgaatttg atatttatga cgatgaacaa
     7021 gaacttaaat taaaaattta tgaacaaatt atgttaaaaa tagagtccga atacaaggat
     7081 atctatttat tccgtcttat taattgattt actgtattcg gttaatattc tttcgttttc
     7141 atcaacgatg tcctttagtg tgtttaaaag gaagtcgcaa tcacctttgg ctactgcacc
     7201 ggcttgtgaa tggttgatta tatttctcat actataagca atttctaccc gttttttggt
     7261 tctataattc actttaccct ctttagttaa ttctcctaat aatttggtgt acatagttga
     7321 atcggtgtct ttatgtttga ttttattcac tttttttaat ttgattaaaa acgtctctat
     7381 agcaacagca aaggttgctg cagctggcaa atacaactcc cttttataag cttgtaatcc
     7441 ttgttctatt tgataagaaa aagttatatc atcaacaatc tttttcatgc tatttaaatc
     7501 taagtggttg aacggttgta tttcatcatg tgctttgttt atcaatttct ctttcgactt
     7561 cgatatcaat gtattgtaat gatcgttagc taatcgtttg ccataattaa aaaataaatc
     7621 taaattgttt tgtattatta cagtcccgat atattttccg tagtaaatag acgtgtaata
     7681 aatgtagtta ttaaaatcta ataatccgga ttgttcttct acatactttt tagaatcata
     7741 tatgtatgaa gtaaagtgtt tagacaaatg tttgatatca gtattacgaa aattatatat
     7801 ttcttttaat ttactgtcat ttgagataac aacgatgcaa ggttcttcaa aaaaagattg
     7861 atttagataa aatatcgaaa tcttgtaatc gtcttttctc atgaatggga aggcttccgg
     7921 attactacta aactgataaa tgtatctgtt ttcaactaca tatttgtaac cttctaaaaa
     7981 attacgcaag tattctttta aagttttatt ctcttccatc cctcatcctc ctcacgccac
     8041 acaagcgcta ttaatcaata tccaataatt gttgtttttt cttatcgaac tcttcctgag
     8101 aaattactcc gacatctaat aattctttat attttattaa ttcatcagca acagaaaaac
     8161 tcattttttc agaattggat ggtttcatag aactttctcg aatagagatt tgttcttgta
     8221 ttgtttccgc cattctagat acagtgtttt ttgatatgct tcctatagcg atacttgatg
     8281 aaccgtgatg tataattatt tcgccaaaaa gaagtccttt tttatacgaa acagaattga
     8341 ttttctcgaa tggaaattca tgaaatttca aaccatatat cataccttta tctaagaata
     8401 acaatcttag atcagtacat actattaagt aggtattatt attgtacaat cccgaagtta
     8461 catacattat gttttcatta tcttttaaaa tcataggtag ttctttcact tctttttttg
     8521 taccaaacaa atcctctaca cctatttcgc taaatctttg gtagatttta gataagtttt
     8581 cgtcagattt attgatttca ctttcaaatt tcacttcttt tctaggttta ctttggtatt
     8641 cttttaaaat ttctcttttg tcttcaacag atagttgctt gtattgtttc ttttcttctt
     8701 ttgttttagt tgctaaatat tgactctcaa tcatactttc tttgaacgtt aatctgctct
     8761 taggtaattc tttcatgttc atttctcctt tgtttatatt tccttatatt taaaa
//
LOCUS       DAMZKC010000110         8693 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.111, whole genome shotgun sequence.
ACCESSION   DAMZKC010000110 DAMZKC010000000
VERSION     DAMZKC010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8693
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.111"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(211..486)
                     /locus_tag="POB55_001883"
     CDS             complement(211..486)
                     /locus_tag="POB55_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HDI6500843.1"
                     /translation="MAKQATMKNAALKQLTKDADEILHLIKVQLDNLTLPSCPLYEEV
                     LDTQMFGLQKEVDFAVKLGLVDREDGKQIMLRLEKELSKLHEAFTLV"
     gene            627..1109
                     /locus_tag="POB55_001884"
     CDS             627..1109
                     /locus_tag="POB55_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500844.1"
                     /translation="MIQIKGAVKFPITLDSTTWIFDDRKVSIEDLEKGVFDGSKPIDF
                     EDNHEWNRAILEGQTNPPTLNSEIKYKKRAMLEGSFVINMTPFFKHSEPHDSATTIRL
                     SNEHDHLDIPIDLLPYLFFQFAKDGKRLYSDNGVDSFIYNQEDGYSYRINHVTHIEVI
                     "
     gene            1111..1278
                     /locus_tag="POB55_001885"
     CDS             1111..1278
                     /locus_tag="POB55_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaI family protein"
                     /protein_id="HDI6500845.1"
                     /translation="MRQVQCIICDAKVFIDERTTESKRLKNNPIRTFMCDDCKSRLDT
                     PKQRAQHYPLD"
     gene            complement(1418..3265)
                     /gene="typA"
                     /locus_tag="POB55_001886"
     CDS             complement(1418..3265)
                     /gene="typA"
                     /locus_tag="POB55_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HDI6500846.1"
                     /translation="MTNKREDVRNIAIIAHVDHGKTTLVDELLKQSGIFRENEHVDER
                     AMDSNDIERERGITILAKNTAVDYKGTRINILDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQNLKPVVVVNKIDKPSARPEGVVDEVLDLFIELEANDEQ
                     LEFPVVYASAVNGTASLDPEKQDDNLQSLYETIIDYVPAPIDNSDEPLQFQVALLDYN
                     DYVGRIGIGRVFRGKMRVGDNVSLIKLDGTVKNFRVTKIFGYFGLKRLEIEEAQAGDL
                     IAVSGMEDINVGETVTPHDHQEALPVLRIDEPTLEMTFKVNNSPFAGREGDFVTARQI
                     QERLNQQLETDVSLKVSNTDSPDTWVVAGRGELHLSILIENMRREGYELQVSKPQVII
                     KEIDGVMCEPFERVQCEVPQENAGAVIESLGARKGEMVDMTTTDNGLTRLIFNVPARG
                     MIGYTTEFMSMTRGYGIINHTFEEFRPRIKAQIGGRRNGALISMDQGSASTYAILGLE
                     DRGVNFMEPGTEVYEGMIVGEHNRENDLTVNITKTKHQTNVRSATKDQTQTMNRPRIL
                     TLEEALQFINDDELVEVTPESIRLRKKILNKNVREKEAKRIKQMMQENE"
     gene            3367..3558
                     /locus_tag="POB55_001887"
     CDS             3367..3558
                     /locus_tag="POB55_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaF family protein"
                     /protein_id="HDI6500847.1"
                     /translation="MKQKKSKNIFWVFSILAVVFLVLFSFAVGASNVPMMILTFILLV
                     ATFGIGFTTKKKYRENDWL"
     gene            complement(3712..4539)
                     /locus_tag="POB55_001888"
     CDS             complement(3712..4539)
                     /locus_tag="POB55_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HDI6500848.1"
                     /translation="MALYGFAQGLIQEAGIRIKQLMEQNLTIETKSNPNDLVTNVDKA
                     TEDFIFDTILETYPNHQVLGEEGHGHDIDTSKGTVWVVDPIDGTLNFVHQQENFAISI
                     GIYIDGKPYAGFVYDVMADVLYHAKVGEGAYRGSQPLKPLNDSNLRQSIIGINPNWLT
                     KPILGEIFKEIVNDSRSARAYGSAALEIVSVATGNLEAYMTPRLQPWDFAGGLVILYE
                     VNGQASNLLGEPLTISGPNSILVGNRGLHQEISNDYLEPHHDALIQLHEQRFKRKSK"
     gene            4696..5310
                     /locus_tag="POB55_001889"
     CDS             4696..5310
                     /locus_tag="POB55_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YktB family protein"
                     /protein_id="HDI6500849.1"
                     /translation="MTKYTFKPKDFKAFNVEGLDARMEALNEYIRPQLHELGEYFSDF
                     FTSQTGETFYPHVAKHARRSVNPPKDTWVAFATNKRGYKMLPHFQIGMFEDQLFVMFG
                     IMHEAKDKATRAKVFERNFKAIQQLPDDYRVCLDHMKPDKPFIKDLTDDDLKEAIQRA
                     INVKKGEFFIARAITPQDKRLKSDKAFIAFLEETFDQFLPFYSA"
     gene            5494..6846
                     /locus_tag="POB55_001890"
     CDS             5494..6846
                     /locus_tag="POB55_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HDI6500850.1"
                     /translation="MNNKRHSTNEQLSLDEINNTIKFDHRSSNKQKFLSFLGPGLLVA
                     VGYMDPGNWITSMQGGAQYGYTLLFVILISSLSAMLLQSMTVRLGIATGMDLAQMTRH
                     YLSRPIAIIFWIIAELAIIATDIAEVIGSAIALNLLFNIPLIVGALITVLDVFLLLFI
                     MKYGFRKIEAIVGTLIFTVLFIFIFEVYISSPQLNAVLNGFIPHSEIITNNGILYIAL
                     GIIGATIMPHNLYLHSSIVQSRTYSRHNNEEKAQAIKFATIDSNIQLSIAFVVNCLLL
                     VLGASLFFNSNADDLGGFYDLYHALKTEPVLGATMGAIMSTLFAVALLASGQNSTITG
                     TLAGQIVMEGFLRLHIPNWLRRLITRSLAVIPVIVCLIIFKGNAAKIEQLLVFSQVFL
                     SIALPFCLIPLQLATSNKDLMGPFYNKTWVNIISWTLIIILSILNVYLIVQTFQELQS
                     "
     gene            complement(6928..7347)
                     /locus_tag="POB55_001891"
     CDS             complement(6928..7347)
                     /locus_tag="POB55_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HDI6500851.1"
                     /translation="MNRKPELIMAWIANSISIIYLLVMGLSYFSLKSGNASQREELAK
                     QLSQNGGKVSLDMLQTTMGALAIILLISTLYGIFATICIKGRRKLSIILFVIAIIVSL
                     MALNLIAIVLWVIVMIMLISKKESKETTHKDDEYIYH"
     gene            complement(7630..8649)
                     /locus_tag="POB55_001892"
     CDS             complement(7630..8649)
                     /locus_tag="POB55_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HDI6500852.1"
                     /translation="MTGEQFTQIKRPVSRLTEKVLGWLCWVMLLVLTVITMFIALVSF
                     SNNTSIANLENTLNNNAFIQQLLAGNGYNTTQFVIWLQNGIWAIIVYFIVCLLISFLA
                     LISMNIRILSGFLFLISAIVTIPLVLLIVTLIIPILFFIIAMMLFIRKDKVEMVAPQY
                     YEEYNGPIYDYREPVYERPQPKDDYYDVPKYEKELDKSNTVYDQEQERDKYDQFPKRA
                     VESEYNHDERTEEEPSVLSRQAKYKQKSTEELGIEDDGYYAEPEVDPKELKAQQKREK
                     AEIKAKKKEKRKAYNQRMKERRKNQPSAVSQRRMNFEERRQIYNNDISEERNSSEVKD
                     KKEQE"
BASE COUNT         2877 a         1520 c         1259 g         3037 t
ORIGIN      
        1 tcacaaatat taacaggtct cttgaggtaa tttatagaat atttgtttac ttaatatcgt
       61 gaattgctaa aacccaataa aagtgactga catgtcatca agaagacaac ataacagcca
      121 ctttaatcag taattaaact ataaatataa aatcggcgca gctcaattgt cgatatctca
      181 cgccatttta atatgcttat atattaaacc ttaaacaagt gtaaaagctt catgtaattt
      241 tgaaagttct ttctcaagac gtaacataat ttgtttgcca tcttcgcggt caactaaacc
      301 taatttaaca gcaaaatcaa cttctttttg aagtccaaac atttgtgtat ctagtacttc
      361 ttcatataat gggcatgaag gtaatgttaa attatctagt tgaactttaa tcagatgcaa
      421 gatttcatca gcatctttag tcaattgttt caaagctgca tttttcattg ttgcttgttt
      481 cgccatgaca cgctccccct ctatataaat tctcaagtct tactaaaagt ttatcttact
      541 aaatattaaa aagcaagtag atttaaaatg tgtcgttagc aatttgtgtt aaaataatgc
      601 tatatacatt gatagggagt gggaaaatga tacaaattaa aggagccgta aagtttccta
      661 taacattaga tagtacgact tggattttcg atgatagaaa agttagcatc gaagatttag
      721 aaaaaggtgt atttgatggt tcaaaaccaa tcgactttga agataatcat gaatggaatc
      781 gtgctatcct agaaggacaa actaatccgc ctactttaaa ttcagaaata aaatataaaa
      841 aacgtgccat gttagaaggt tcatttgtca ttaatatgac accttttttc aaacattccg
      901 aaccacatga ctctgcaact actattcgat tatcaaatga gcatgatcat ttagatatac
      961 cgatagatct attaccctat ttatttttcc aatttgcaaa agacggtaaa cgactttaca
     1021 gcgataatgg tgtagatagt tttatttaca atcaagaaga tggttattca tatcgaatca
     1081 accatgtaac tcatattgag gtgatttaat ttgagacaag tgcaatgcat tatctgtgat
     1141 gcaaaagtat ttatagatga gcgcacaact gagtctaaac gacttaaaaa caatcctatt
     1201 cgaacattta tgtgtgatga ttgtaaaagt cgattagaca cacctaaaca acgtgcgcaa
     1261 cactatccgc tcgattaata tctgaataaa caagttagaa atttgaaaat gtgaaagtaa
     1321 caggaaataa tagcatttaa acactttttc aaatattata tgccaaaaga gaagctaagc
     1381 aactttcatc gctcagcttc tcttttatta tgttaaatta ttcgttttct tgcatcattt
     1441 gtttgatacg ctttgcttct ttttcacgaa catttttgtt taaaattttc tttcttaaac
     1501 gtatgctttc tggtgtaacc tcaacaagtt catcatcatt aatgaattgt aacgcttctt
     1561 ccaatgttag aatacgcggt ctattcattg tttgtgtttg gtctttcgtt gcagaacgta
     1621 cgttagtttg atgttttgtt ttagtgatgt taacagttaa atcattttca cgattatgtt
     1681 caccaacaat cataccttca taaacttcag taccaggttc catgaagttt acacctctat
     1741 cttcaagtcc caaaatggca taagtacttg cagaaccttg atccattgaa attaatgcac
     1801 cattacgacg accgccaatt tgtgctttaa tacgtggtct aaattcttca aatgtatggt
     1861 taataatacc gtaacctctt gtcattgaca taaattcagt cgtataacca atcataccac
     1921 gagccggtac attaaagatt aaacgtgtaa gtccattatc agttgtagtc atatcaacca
     1981 tttcaccttt acgtgcacct aatgattcaa taacagcacc tgcattttct tgtggcactt
     2041 cacattgcac acgttcaaat ggttcacaca ttacaccatc tatttcttta ataattactt
     2101 gtggttttga aacttgtaat tcataacctt cacgacgcat attttcaata aggattgata
     2161 aatgcaattc accgcgacca gcaactaccc atgtatctgg agaatctgtg ttagaaactt
     2221 tcaaagatac atctgtttct aattgttgat ttaaacgttc ttgaatttga cgcgctgtta
     2281 caaagtcacc ttcacggcca gcaaatggag aattgttaac tttaaatgtc atttcaagag
     2341 taggctcatc aatacgtaga actggcaatg cttcttgatg gtcatgtggt gttacagttt
     2401 caccaacatt aatgtcttcc atacctgaaa cagcaattaa atctccagct tgtgcttctt
     2461 caatttctaa acgttttaat ccaaagtaac caaagatttt agttacacgg aagtttttca
     2521 ctgtaccgtc taatttaatt agtgatacat tatctccgac acgcatttta cctctgaata
     2581 cacgaccaat accaatacgt ccaacataat cattgtagtc caacaatgct acttggaatt
     2641 gtaatggctc atcactgtta tcaattggag ctggtacata atcaataatt gtttcatata
     2701 atgattgtaa attatcatct tgtttttcag gatctaagct agctgttcca tttactgctg
     2761 aagcataaac aacagggaat tctaattgtt catcgtttgc ttctaattca ataaataaat
     2821 ctaaaacttc atctacaaca ccctctggac gtgctgatgg tttatcaatt ttattaacaa
     2881 caacaacagg tttcaggttt tgttctagcg cttttttaag tacaaaacgt gtttgaggca
     2941 ttgtaccttc atacgcatct actactaaga caaccccatc aaccattttc ataatacgtt
     3001 ctacttctcc accaaagtct gcatgtcctg gtgtatccaa aatattaata cgtgtacctt
     3061 tataatcaac agccgtattt ttggctagaa tcgtaattcc acgctctctt tcgatatcgt
     3121 tagagtccat tgcacgttca tcgacatgtt cattttctct gaatatacca gattgtttta
     3181 acaactcatc tactaaagtt gttttaccat ggtcaacgtg agcaataatt gctatattgc
     3241 ggacatcttc tcttttatta gtcatataaa tagtttcctt ctttcttaag taaaatccca
     3301 cttttaatta caactttttt attatatcat ataattgaga aaagataagc aaaaggtggg
     3361 gataggatga aacaaaaaaa atctaaaaat atcttttggg tattttctat attagctgtt
     3421 gttttcttag tattatttag ttttgctgtt ggtgcatcaa atgtaccaat gatgatttta
     3481 acatttatat tactcgttgc aacctttgga attggattta ctacaaagaa aaaatatcga
     3541 gaaaacgatt ggctataagt tatatcgaca ataacacgcg ttgatttaag accacacatg
     3601 ttcatgatga acatttaact ggttttatat tggcgcgtgt ttttattatt taaggtataa
     3661 aagcgtaata tgttacctaa ttgttaggac tacatattac gcttttggta attattttga
     3721 ttttctttta aatcgttgtt catgtaattg tattaacgca tcatggtggg gctctaaata
     3781 atcattgcta atttcttgat ggagaccacg atttccaact aagattgaat ttggaccact
     3841 aattgttaat ggttctccta gtaaattgga agcttgtcca tttacttcat ataaaataac
     3901 caatccgcca gcaaaatccc atggttgaag tcttggcgtc atatatgctt ctaaattacc
     3961 tgtagcaact gaaacgattt caagcgctgc actaccatat gcccttgcac ttctagaatc
     4021 attaacaatt tctttaaaga tttctcctaa aattggttta gttaaccagt tcggattgat
     4081 cccaataatg ctttgtctta gattagaatc attcaatggt ttcaagggtt ggctaccacg
     4141 atatgcacct tcccctactt tagcatgata taagacatca gccataacat catatacaaa
     4201 acctgcataa ggtttaccat cgatataaat accaattgaa attgcgaaat tttcttgttg
     4261 atgaacaaaa ttcaatgtac cgtctattgg gtcaacaacc cataccgtac ctttggaagt
     4321 atcgatgtca tgaccatgcc cttcttcacc taatacttga tgattgggat atgtttctaa
     4381 aattgtatca aaaataaaat cttctgttgc tttatctaca tttgtaacaa ggtcattcgg
     4441 atttgacttt gtttcaattg ttaaattttg ctccatcaat tgtttaattc taattcctgc
     4501 ttcttgaata agtccttggg caaatccata aagtgccata acatcatctc ctaacatctt
     4561 tattatacat caacatttta taaaaataac ttcttatgat aaaaatgaaa atactaattt
     4621 taatgagagc gtcttaagcc gcaattgata aaacatatgc tacaatattt ttaaacacta
     4681 tacaggaggt actctatgac aaaatataca tttaaaccta aagatttcaa agcgttcaac
     4741 gtagaaggct tagacgcacg aatggaagct ttaaacgaat acatacgacc acaactccat
     4801 gaattaggag aatattttag tgacttcttt acaagtcaaa ctggtgaaac attttatcct
     4861 cacgtagcaa agcatgctag aagaagtgtg aatcctccta aagatacatg ggttgctttt
     4921 gcaacaaaca aaagaggcta taaaatgtta cctcatttcc aaattggtat gtttgaagat
     4981 caactgtttg ttatgtttgg aatcatgcat gaagcaaaag ataaagcaac acgtgcaaaa
     5041 gtttttgaaa gaaactttaa agctattcaa caattacctg atgattatcg tgtttgctta
     5101 gatcatatga aacctgataa accatttatt aaagatttaa cggatgatga tttaaaagaa
     5161 gcgatacaaa gagccatcaa tgtgaaaaaa ggtgaattct ttatagcgcg tgcaatcaca
     5221 ccacaagata aaagattaaa aagtgacaaa gcatttattg catttttaga agaaaccttc
     5281 gatcagttct taccatttta ttctgcataa ataactttgt ttaaataata gagcacttaa
     5341 tcacatccat gatttcgtgc tcttttttct taatattaaa tcgaacgttc aacataataa
     5401 ttcatacttt taaaaaaatt aaaataaatt taggttgacc taaacatttt attaggttat
     5461 tatattgtcc ataagaagta gaggtgagtc aaaatgaata ataaacgaca ttcaacaaat
     5521 gaacaattaa gtttagacga aataaacaat acaattaaat tcgatcatcg cagttcaaat
     5581 aaacagaaat ttttatcatt tcttgggcct gggttattag tcgctgttgg ttacatggat
     5641 cccggaaact ggataacatc aatgcaaggt ggcgcgcaat atggctatac tttgctattc
     5701 gtaattctta tttcaagttt atcagcaatg ttacttcaaa gcatgacagt gagattagga
     5761 atagcaacag gtatggactt agctcaaatg acacgacatt atttatcaag acctattgct
     5821 ataatctttt ggatcattgc agaactagca attatcgcta cagatattgc tgaggttatt
     5881 ggtagtgcta ttgctcttaa tctcctattt aacatacctt taatcgtcgg tgcactaata
     5941 actgtacttg atgtattttt actactattt ataatgaaat atggttttag aaaaattgaa
     6001 gctattgttg gtacattaat tttcacagtg ttattcatct ttatatttga agtctatatt
     6061 tcatcaccac agttgaatgc tgtgttaaat ggatttatac cacatagtga aatcattaca
     6121 aataacggca ttctctatat tgcattaggt attattggcg ctacaattat gcctcataat
     6181 ttgtacttac attcatcaat tgtacaatct agaacatact caagacataa caatgaagaa
     6241 aaggcgcaag cgattaaatt tgctacgata gattcgaaca ttcagttatc aatcgcattt
     6301 gtagtcaatt gcttattatt agtgttaggt gcatcactat ttttcaactc aaacgctgac
     6361 gatttaggtg gtttctatga tttatatcac gccttaaaaa ctgaacctgt actaggtgca
     6421 acaatgggtg caatcatgag tacattattt gcagttgcat tattagcgtc aggtcaaaat
     6481 tcaacgatta ctggtacttt agcaggacaa attgtaatgg aaggattttt aagattacac
     6541 ataccaaatt ggttaagacg tttaattaca cgttcccttg ctgtcattcc tgttatcgta
     6601 tgcttaatca tttttaaagg taatgcagct aaaattgagc aactacttgt attctctcaa
     6661 gtatttttaa gtattgctct gccattctgt ttaataccat tacaattggc tactagtaat
     6721 aaagacttga tgggaccttt ctataataaa acatgggtta atatcatttc atggacactt
     6781 attattattc taagtatttt aaatgtctat ttaatcgttc aaacattcca agaactacaa
     6841 agttaaaaat aaaaccatta aaatgctggt tgtatctaat aattaacaac ttaacatttt
     6901 aatggttttt cttttgctat gaacatatta atgataaata tactcatcgt ccttatgtgt
     6961 agtttctttt gattcttttt tagaaatcaa cataatcatc acgataaccc ataagacaat
     7021 tgcaattaaa ttaagagcca tcaaacttac aattatcgcg ataacaaaaa gtataatcga
     7081 taattttcta cgtcctttaa tacaaattgt cgcaaatata ccataaagtg ttgaaattaa
     7141 taaaataatt gctaatgcac ccattgttgt ctgaagcata tctaaagaaa ccttgccacc
     7201 gttctgagat aattgcttcg ctaattcttc acgttgtgat gcattaccac tttttaaaga
     7261 aaaataggat agccccataa ctaataaata aataatacta atactatttg caatccaagc
     7321 catgattaat tcaggttttc tattcatctt aaaatccttt ctttgtttaa tattttaata
     7381 agtatactac gagcctatag atatacgcac attaaattac ttgtatatta acatattttt
     7441 catctaattt ttataataaa catctgaatt cagattgatg ataatttagg tgtatagtaa
     7501 tgagaaatcg tcatttaaaa ttatgacacc taaacacttt aattaaacgt tatcgataat
     7561 taacctcata taaaaaccca gattaataag caaaagacct ctgcttacta atctgggtat
     7621 catcaatatt tattcttgct cttttttgtc cttaacttca cttgaattgc gttcttcaga
     7681 aatatcattg ttgtaaattt gacgtcgctc ttcaaaattc attcgacgtt gactaactgc
     7741 gctaggttga tttttcctac gttctttcat tcgttggtta taagctttac gtttttcttt
     7801 tttcttagct ttaatttcag ctttttctcg cttttgttgt gctttcaatt cttttggatc
     7861 aacttcaggt tctgcataat aaccatcatc ttcaatacct agttcttcag tacttttttg
     7921 tttgtattta gcctgtctgg ataatactga tggttcttcc tcagtgcgct catcatgatt
     7981 atattcactt tcaactgcac gtttaggaaa ttgatcatat ttatctcttt cctgttcttg
     8041 atcatataca gtatttgatt tatccaattc tttttcatat ttaggcacat cataataatc
     8101 atctttcggt tgggggcgct catacacagg ttcacgataa tcataaatcg gtccgttata
     8161 ctcttcataa tattgtggcg caaccatttc aactttatct tttcttataa atagcatcat
     8221 cgcaataata aagaataata tcggaatgat taaagtaacg ataagtaaaa ctaaaggaat
     8281 cgttacaatt gctgatatta agaataagaa acctgacaag attcttatat tcatagatat
     8341 gagagctaaa aatgaaatca acaaacaaac aataaagtaa acgataatag cccatatacc
     8401 attttgtaac catattacaa attgtgttgt attatagcca tttccagcta ataattgctg
     8461 gataaatgca ttattgttta atgtattttc aagattagca atcgatgtgt tattactgaa
     8521 tgaaacaagt gctataaaca tcgtaatgac agtaagcact aataacatca cccaacataa
     8581 ccaacctaga actttttcag ttaatctact tactggacgt ttaatttgag taaattgttc
     8641 tccagtcatt cgttacaact ccttatagta cttatcccgt tattataact aaa
//
LOCUS       DAMZKC010000111         8467 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.112, whole genome shotgun sequence.
ACCESSION   DAMZKC010000111 DAMZKC010000000
VERSION     DAMZKC010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8467
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.112"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            43..834
                     /gene="pgeF"
                     /locus_tag="POB55_001893"
     CDS             43..834
                     /gene="pgeF"
                     /locus_tag="POB55_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HDI6500853.1"
                     /translation="MNDNFKKQPHHLIYEELLQQGITLGITTRGDGLSDYPKNAFNMA
                     RYIDDRPYNITQHQLQLAEEIAFDRKNWVFPIQTHENKVACITKDDIGTNIDTLTDAL
                     HGIDAMYTYDSNVLLTMCYADCVPVYFYSTKHHFIALAHAGWRGTYTEIVKEVLKHVN
                     FDLKDLHVVIGPSTSSSYEINDDIKNKFETLPIDSANYIETRGRDRHGIDLKKANAAL
                     LNYYGVPKENIYTTAYATSEHLELFFSYRLEKGQTGRMLAFIGQQ"
     gene            858..1526
                     /locus_tag="POB55_001894"
     CDS             858..1526
                     /locus_tag="POB55_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HDI6500854.1"
                     /translation="MKDNLQQISTQINDKSEKNNFSTKPNVIAVTKYVTIERAKEAYE
                     AGIRHFGENRLEGFLQKKEALPSDAVIHFIGSLQSRKVKGVINDVDYFHALDRLSLAK
                     EINKRAEHKIKCFLQVNVSGEASKHGIALEDVDQFIDDLKKYDKIEIVGLMTMAPLTD
                     DEAYIRSLFKQLRLKKEEIQRLNLEYAPCDELSMGMSNDYLIAVEEGATFVRIGTKLV
                     GEEE"
     gene            1523..2086
                     /locus_tag="POB55_001895"
     CDS             1523..2086
                     /locus_tag="POB55_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HDI6500855.1"
                     /translation="MSHLALKDLFSGFFVIDDEEEVEVPDKQQQVNEAPAKEQSQQTT
                     KQNAIKSVPQKSASRYTTTSEERNNRMSNYSKNNSRNVVTMNNATPNNASQESSKMCL
                     FEPRVFSDTQDIADELKNRRATLVNLQRIDKVSAKRIIDFLSGTVYAIGGDIQRVGTD
                     IFLCTPDNVEVAGSITDHIENMEHSFD"
     gene            2098..2388
                     /locus_tag="POB55_001896"
     CDS             2098..2388
                     /locus_tag="POB55_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HDI6500856.1"
                     /translation="MDINVLATIFKFILFVVEIYYFGMIIYFFTSWVPSIRETKVGYF
                     LAKIYEPFLQPFRKVIPPIGIIDISSIAAIIVLVLFQKGLLQIFNWILIQLQ"
     gene            2498..3277
                     /locus_tag="POB55_001897"
     CDS             2498..3277
                     /locus_tag="POB55_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001827493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HDI6500857.1"
                     /translation="MINIDIYQHFRQEEYELIDQLTDKCDQAEQHYAPVLTHFLDPRG
                     QYILEVICGSYEDLNVSFYGGPNAERKRAIISPNYYEPKESDFELTLMEIDYPEKFVT
                     LKHQHILGTLMSLGIEREQVGDIIVNERIQFVLTSRLESFIMLELQRIKGASVKLYTI
                     PVTDMIQSNENWKNESATVSSLRLDVVIKEMIRKSRTIAKQLIEKKRVKVNHTIVDSA
                     DFQLQANDLISIQGFGRAHITDLGGKTKKDKTHITYRTLFK"
     gene            3301..3918
                     /locus_tag="POB55_001898"
     CDS             3301..3918
                     /locus_tag="POB55_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HDI6500858.1"
                     /translation="MPFTPNEIKNKEFSRVKNGLEPTEVANFLEQLSTEIERLKEDKK
                     QLEKVIEERDTNIKSYQDVHQSVSDALIQAQKAGEETKQAAEKQAEAIIAKAEAQANQ
                     MVGDAVEKARRLAFQTEDMKRQSKVFRSRFRMLVEAQLDLLKNEDWDYLLNYDLDAEQ
                     VTLENIHHLHENDLKPDEVAANAQNNASNTSDNNQQSNDSETTKK"
     gene            4139..6892
                     /gene="ileS"
                     /locus_tag="POB55_001899"
     CDS             4139..6892
                     /gene="ileS"
                     /locus_tag="POB55_001899"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HDI6500859.1"
                     /translation="MDYKETLLMPKTDFPMRGGLPNKEPQIQEKWDAEDQYHKALEKN
                     KGNETFILHDGPPYANGNLHMGHALNKILKDFIVRYKTMQGFYAPYVPGWDTHGLPIE
                     QALTKKGVDRKKMSTAEFREKCKEFALEQIELQKKDFRRLGVRGDFNDPYITLKPEYE
                     AAQIRIFGEMADKGLIYKGKKPVYWSPSSESSLAEAEIEYHDKRSASIYVAFDVKDDK
                     GVVDADAKFIIWTTTPWTIPSNVAITVHPELKYGQYNVDGEKYIIAEALSDAVAEALD
                     WDKASIKLEKEYTGKELEYVVAQHPFLDRESLVINGDHVTTDAGTGCVHTAPGHGEDD
                     YIVGQKYELPVISPIDDKGVFTEEGGQFEGMFYDKANKAVTDLLTEKGALLKLDFITH
                     SYPHDWRTKKPVIFRATPQWFASISKVRQDILDAIENTNFKVNWGKTRIYNMVRDRGE
                     WVISRQRVWGVPLPVFYAENGEIIMTKETVNHVADLFAEHGSNIWFEREAKDLLPEGF
                     THPGSPNGTFTKETDIMDVWFDSGSSHRGVLETRPELSFPADMYLEGSDQYRGWFNSS
                     ITTSVATRGVSPYKFLLSHGFVMDGEGKKMSKSLGNVIVPDQVVKQKGADIARLWVSS
                     TDYLADVRISDEILKQTSDVYRKIRNTLRFMLGNINDFNPDTDSITESELLEVDRYLL
                     NRLREFTASTINNYENFDYLNIYQEVQNFINVELSNFYLDYGKDILYIEQRDSHIRRS
                     MQTVLYQILVDMTKLLAPILVHTAEEVWSHTPHVKEESVHLADMPKVVEVDQALLDKW
                     RTFMNLRDDVNRALETARNEKVIGKSLEAKVTIASNDKFNASEFLTSFDALHQLFIVS
                     QVKVVDKLDDQATAYEHGDIVIEHADGEKCERCWNYSEDLGAVDELTHLCPRCQQVVK
                     SLV"
     gene            7182..7979
                     /locus_tag="POB55_001900"
     CDS             7182..7979
                     /locus_tag="POB55_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDI6500860.1"
                     /translation="MYHNSNANFVNGITLNVRDKNELKPFYEDILGLNIINETLTSIQ
                     YEVGQNNHVITLVELQNGREPLMSEAGLFHIAIKLPQISDLANLLIHLSEYDIPVNGG
                     IQPASLSLFFEDPEGNGFKFYVDKDEAQWTRQNDLVKIDIRPLNVPRLVSHATKLLWL
                     GIPDDAIIGALHIKTIHLSEVKEYYLDYFGLEQSAYMDDYSIFLASNGYYQHLAMNDW
                     VSATKRVENFDTYGLAIVDFHYPETTHLNLQGPDGIYYRFNHIEVED"
BASE COUNT         3072 a         1217 c         1599 g         2579 t
ORIGIN      
        1 gttagacgaa gataatttaa atgaaatgat ggtgacgatc gagtgaatga taattttaaa
       61 aagcaaccgc atcatttaat atatgaagag ttattacaac aaggtattac tctaggtatt
      121 acaactagag gcgatggttt aagtgactat cctaaaaatg cttttaatat ggcgagatat
      181 attgatgatc gcccatataa tattactcaa catcaattgc aattagctga agaaattgcg
      241 tttgatagaa aaaattgggt gtttcccatt caaacacatg aaaataaagt cgcttgtatt
      301 acaaaggatg atataggcac aaatatagac actttaactg atgcgcttca tggtattgat
      361 gcgatgtaca catatgatag taatgtctta ttaacgatgt gttatgcaga ctgtgtacca
      421 gtatattttt atagtacaaa acatcatttt attgcattgg cgcatgcagg ttggcgtggt
      481 acctatactg aaattgtaaa agaagtgcta aaacatgtga actttgattt gaaagactta
      541 catgtcgtta ttggaccatc tacatcatca agttatgaaa ttaatgatga tattaaaaat
      601 aaatttgaaa cattgccaat tgatagtgcc aactatattg aaactagagg acgagatcgt
      661 catggtattg atttgaaaaa agccaatgct gcattattaa attattatgg tgttcctaaa
      721 gaaaatattt atacgacagc gtatgctaca tctgaacatt tagaattatt tttctcttat
      781 cgattagaaa aaggtcaaac aggacgcatg ttagcattca ttggtcaaca gtaaacaagg
      841 aggagatatg cttgcgtgtg aaagataatt tacaacaaat ctcaacacaa attaatgaca
      901 aaagtgaaaa aaataatttt tcaacaaaac caaacgtgat tgcagttaca aaatatgtta
      961 caatagagcg agctaaagaa gcgtatgagg ctggaataag acattttggt gagaatagat
     1021 tggaaggctt tttacaaaag aaagaagcat taccatcaga tgcggtgatc cattttatag
     1081 gatcattaca atctcgaaaa gttaagggcg ttataaacga cgtagattat ttccatgctt
     1141 tagatcgatt gagcttagcc aaagaaatta acaaacgtgc agaacataaa attaaatgtt
     1201 tcttgcaagt gaacgtttcg ggagaagctt ctaaacatgg tattgcttta gaagatgttg
     1261 atcagtttat agatgatctt aaaaaatatg acaaaatcga aattgtaggt ttaatgacga
     1321 tggcaccatt gacagatgat gaagcatata ttagatcgtt atttaaacag ttacgtttga
     1381 aaaaagaaga aatacaacga ctcaatttag aatatgcgcc ttgtgatgaa ttatcaatgg
     1441 gaatgagtaa tgactatctt attgcagttg aagaaggtgc gacgtttgtt agaattggga
     1501 ctaaacttgt aggagaagag gagtgagcca cttggcttta aaagatttat ttagtggatt
     1561 ttttgtaata gatgatgaag aggaagtaga agtacctgac aaacaacaac aggtcaatga
     1621 agcgccagca aaagagcagt cacaacaaac aacaaaacaa aacgcaatca aatcagtccc
     1681 tcaaaaatct gcatcaagat atacaacaac gtcagaagaa aggaataacc gtatgtctaa
     1741 ttattcaaaa aataattcac gtaatgttgt aactatgaac aatgctacac caaacaatgc
     1801 atcacaagaa agttcaaaaa tgtgtttatt cgaaccacgt gttttttcag atacacaaga
     1861 tattgctgat gagcttaaaa accgccgtgc gacacttgtc aatttacaac gtattgataa
     1921 agtatcagcg aaaagaatta ttgatttttt aagcggtact gtttatgcaa tcggtggaga
     1981 tatccaacgt gtaggtactg atattttctt atgtacgcct gataatgtgg aagtagctgg
     2041 aagcattaca gaccatattg aaaatatgga acattcattc gactaaggag ttaacatatg
     2101 gatataaatg tgctagctac aatatttaaa tttatccttt ttgttgttga aatttattat
     2161 ttcggcatga ttatatattt ctttacatct tgggtaccaa gtattagaga aactaaggta
     2221 ggttattttt tagcgaaaat atatgaacct ttcttacaac catttagaaa agtaattcca
     2281 cctattggaa ttatcgacat atcatcaatc gctgcaatta tcgttttagt attattccaa
     2341 aaagggttac tccaaatctt taattggatt ttaattcaat tacaataaat taagcgacat
     2401 aacgaggcaa gggtacatga tactttagcc tcgtttttga tatgtatttt tctgaatata
     2461 agggcaatag atggtatttt ataatttttt taaggtagtg attaacatag atatttatca
     2521 acactttaga caggaagaat acgaattaat tgatcagcta acggataaat gtgatcaagc
     2581 ggaacagcat tatgcaccag tattaacgca ttttttagat ccaagagggc aatatatatt
     2641 ggaagtgatt tgtggcagtt atgaagattt aaacgtatct ttttatggtg gacctaatgc
     2701 tgaaagaaaa agagcaatca tttcgccgaa ctattatgaa cctaaagaaa gcgactttga
     2761 attaacttta atggaaatag attatcctga aaaattcgtc actttaaaac atcaacatat
     2821 tttagggaca ttaatgtctt taggtatcga acgcgaacaa gttggagata taattgtgaa
     2881 tgaacgaatt caatttgttt tgacaagtag attggaatca tttattatgt tagaattaca
     2941 acgtattaaa ggcgcatcag ttaaacttta tactattcca gtaacagata tgatacaatc
     3001 taatgagaat tggaaaaatg aaagtgcaac agttagttct ttaaggttag atgttgttat
     3061 taaagaaatg atacgtaaat cacgtacgat tgcgaaacaa ctaatcgaaa aaaaacgtgt
     3121 taaagtgaat cacactattg ttgattcagc agattttcaa ttacaagcaa atgatttaat
     3181 atccatccaa ggttttggta gagcacacat tactgactta ggtggtaaaa ctaaaaaaga
     3241 taaaacgcac attacctata gaacattatt caaatagtaa tgatttaagg aggataacaa
     3301 atgcctttta caccaaatga aattaagaat aaagagtttt cacgtgtaaa gaatggttta
     3361 gaacctactg aagttgctaa ttttttggag caactaagca ctgaaattga acgtcttaaa
     3421 gaagataaaa aacaacttga aaaagtaatc gaagagagag atactaatat taagtcttat
     3481 caagacgtgc atcaatctgt aagtgatgct ttgatacaag ctcaaaaagc tggtgaagaa
     3541 actaagcaag ctgcagaaaa acaagctgaa gcgattatag ctaaggcaga agcgcaagct
     3601 aatcaaatgg ttggtgacgc ggtagaaaaa gcacgccgtt tagcattcca gactgaagat
     3661 atgaaacgtc aatcaaaagt atttagatcg cgtttccgta tgttagttga agcgcaatta
     3721 gacttattaa aaaacgaaga ttgggattac ttgttgaatt atgatttaga cgctgaacaa
     3781 gtgacgcttg aaaatattca tcatttgcat gaaaatgatt taaagccaga tgaagttgca
     3841 gcaaatgcac aaaataatgc atcaaataca tcagacaata atcaacaatc caatgattca
     3901 gaaacaacta agaagtaaga attaaataaa gacagacgcg taatatacat ttaacttttc
     3961 acagcgaatt aggtaatggt gagagcctag taaaagcatg tatgttatat cactggcttt
     4021 ttaatattta aataatgtaa tgagagaact ctaagttgag ttaataaggg tggtaccgcg
     4081 agcaatcgtc ccttttaatt taacttagag ttttttaaat ttttaaggag tgaaaaaaat
     4141 ggattacaaa gaaacgttat taatgcctaa aacagatttc ccaatgcgag gtggtttacc
     4201 aaacaaggaa ccgcaaattc aagaaaaatg ggatgcagaa gatcaatacc ataaagcgtt
     4261 agaaaaaaat aaaggtaacg aaacattcat tttacatgat ggcccaccat acgcgaatgg
     4321 taacttacat atgggacatg ccttgaacaa aattttaaaa gactttattg tacgttataa
     4381 aactatgcaa gggttctatg caccatacgt accaggttgg gatacacatg gtttaccaat
     4441 tgaacaagca ttaacgaaaa aaggtgttga ccgaaagaaa atgtcaacag ctgaattccg
     4501 tgagaaatgt aaagaatttg ctttagaaca aattgaatta cagaaaaaag attttagacg
     4561 tttaggtgtt cgtggtgact ttaatgatcc atatattaca ttaaaacctg aatacgaagc
     4621 tgcacaaatt cgtatttttg gagaaatggc agataaaggt ttaatttata aaggtaaaaa
     4681 gccagtttat tggtctcctt caagtgagtc ttcattagca gaagcagaaa ttgaatatca
     4741 cgataaacgt tcagcatcaa tttacgttgc atttgacgtt aaagatgaca aaggtgtcgt
     4801 agatgcagat gctaaattta ttatctggac aacaacgcca tggacaattc catcaaatgt
     4861 tgcgattacc gttcatcctg aattaaaata tggtcaatac aatgtagatg gcgaaaaata
     4921 tattattgca gaagccttat ctgacgctgt agcagaagca ctggattggg ataaagcatc
     4981 aatcaaatta gaaaaagaat acacaggtaa agaattagag tatgttgtag cacaacatcc
     5041 attcttagac agagaatcgt tagtgattaa tggtgatcat gttactacag atgctggtac
     5101 aggttgtgta catacagcac caggtcacgg ggaagatgac tatattgttg gtcaaaaata
     5161 tgaattgcca gtaattagtc caatcgatga taaaggtgta tttactgaag aaggcggcca
     5221 atttgaagga atgttctatg ataaagctaa taaagccgtt actgatttat taacagaaaa
     5281 aggtgcacta ttaaaattag actttattac acatagctat ccacacgact ggagaacaaa
     5341 aaaacctgta atcttccgtg ctacaccaca atggtttgcc tcaatcagta aagtaagaca
     5401 agatatttta gatgcaatcg aaaatacaaa cttcaaagta aattggggta aaacacgtat
     5461 ttacaatatg gttcgtgacc gtggcgaatg ggttatttct cgtcaacgtg tgtggggtgt
     5521 accgttacca gtattttatg ctgaaaatgg cgaaattatc atgacgaaag aaacagtgaa
     5581 tcatgttgct gatttatttg cagaacacgg ttcaaatatt tggtttgaaa gagaagcgaa
     5641 agacttacta ccagaaggat ttacacatcc aggcagccct aacggtacat ttactaaaga
     5701 aacagacatt atggacgttt ggtttgattc tggttcatca caccgtggcg tgttggaaac
     5761 aagaccggaa ttaagtttcc cagcggatat gtatttagaa ggtagtgacc aatatcgtgg
     5821 ttggttcaac tcttctatca caacttcagt tgctacaaga ggagtatcac cttataaatt
     5881 cttactttct catggttttg ttatggacgg tgaaggtaag aaaatgagta aatctttagg
     5941 taatgtgatt gtacctgacc aagtggttaa acaaaaaggt gctgatattg cgagactttg
     6001 ggtaagtagt acggactatt tagctgatgt tagaatttct gatgaaattt taaaacaaac
     6061 atctgatgtt tatcgtaaaa tcagaaatac attaagattt atgttaggta acattaacga
     6121 tttcaatcct gacacagata gcattactga atcagagtta ttagaagtgg atcgttactt
     6181 gctaaatcgt ttacgtgaat ttactgcaag tacgattaac aactatgaaa actttgacta
     6241 cttaaatatt tatcaagaag ttcaaaactt tatcaatgtt gagttaagta atttctattt
     6301 ggattacggt aaagatattt tatatattga acaacgagat tctcatatcc gtcgtagtat
     6361 gcaaacagtg ttatatcaaa ttttagttga tatgacgaag ttgttagcac caatcttagt
     6421 gcatacagct gaagaagttt ggtctcatac accacatgtt aaagaagaaa gtgttcactt
     6481 agcagacatg cctaaagttg tagaagtaga tcaagcttta ttggataaat ggcgtacatt
     6541 tatgaattta cgtgatgatg tgaaccgtgc attagaaact gctcgtaatg aaaaagttat
     6601 tggtaaatca ttagaagcta aagttacgat tgctagtaac gataaattta atgcatctga
     6661 attcttaact tcatttgatg cattacatca attatttatc gtgtcacaag ttaaagttgt
     6721 agataagtta gatgatcagg caacagctta tgaacatggt gatattgtca tcgaacatgc
     6781 agatggtgaa aaatgtgaaa gatgttggaa ctattcagag gatcttggtg ctgttgatga
     6841 attgacgcat ttatgcccac gatgccaaca agttgtaaaa tcacttgtat aattgaaatt
     6901 gtataaagta ctcatacaga tgatataaat taaagctctc ttcataatca tgttgtagtt
     6961 tttgttgaca tgatgaagag agtttttttg tgaataaaaa aatgaccaag ttaccggtca
     7021 tatatgtaaa aaatgtgcga tttactaaaa taaaaattat tcaggaatgg tacaaattct
     7081 ctgaggcata taaatgcgtt atagttgcta ttctcaatta tgttcgcgat aattttaagt
     7141 aaaagtaagc acagatattg aatttgatag gagttaattg aatgtatcat aacagtaacg
     7201 caaactttgt caatggtatc actttaaatg tgagagataa gaatgaatta aagccatttt
     7261 atgaggacat attaggatta aatattataa atgagacatt aacatcgata caatatgaag
     7321 taggtcaaaa taatcatgtc attacacttg ttgaattaca aaatggacgt gaacctttaa
     7381 tgtccgaagc gggactgttt catatcgcaa ttaaactacc tcaaattagt gatttagcta
     7441 atttactaat tcatttaagc gaatatgata ttccagttaa cggaggtata cagcctgctt
     7501 cgttatcatt attttttgaa gacccggaag gaaacggttt taaattttat gttgataaag
     7561 acgaagcgca atggacgagg caaaatgatt tagtaaaaat tgatattaga ccattaaatg
     7621 taccgagatt agtgagtcat gcaacaaaat tgttatggtt aggtattcca gatgacgcta
     7681 ttataggtgc attgcatatt aagacaattc atttatcaga ggtaaaagag tactacctcg
     7741 attattttgg attagagcaa tcggcatata tggatgatta ttcaatattt ttagcatcga
     7801 atggctatta tcaacatttg gccatgaatg attgggtatc agcaacgaaa cgtgtagaaa
     7861 attttgatac gtatggatta gcaattgttg actttcatta tcctgaaaca acacatttaa
     7921 atttacaagg tccggatggt atctattatc gctttaatca tatcgaagtt gaagattagt
     7981 atatactttg aatggacgaa ccatataatg aatcgttttt aatgatcttt ttatacaagc
     8041 tatgaaggag gctgggacat aaatccctaa aaaacagcag taagataatt ttcaattaga
     8101 aaatatctta ctgctgttct ctatttatac aatacttcgt attgaatggc ttcgctttgc
     8161 ccatctggca cattactgta aaattctata aatagaattt ttgatgatgg gtcccttcct
     8221 agggtgccgt ctcagctacc ccaaccggca cattgttgta agctgactat atgtcagctt
     8281 ctgtgttggg gcccctgtct tcgactggca ctgctccctc aggagtctcg ccattaatac
     8341 tacgtattaa catgtaattt tactttgaaa tactttaaag cccaaattaa tcaaaagctt
     8401 tctgaaccag aaacgaaaaa atctatagtt aaagaaaaat tgatgtagag cctgtttttg
     8461 gatttat
//
LOCUS       DAMZKC010000112         8266 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.113, whole genome shotgun sequence.
ACCESSION   DAMZKC010000112 DAMZKC010000000
VERSION     DAMZKC010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8266
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.113"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..285
                     /locus_tag="POB55_001901"
     CDS             <1..285
                     /locus_tag="POB55_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500861.1"
                     /translation="SIKGLSEVGQMLVDSDNYAFAYTLDDGKAYAYLIFVQETWTMLH
                     ENMTKKIIINDELELTEFHQELTYILDNIKGNNNYGKEFVATVEETFDIE"
     gene            302..937
                     /locus_tag="POB55_001902"
     CDS             302..937
                     /locus_tag="POB55_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HDI6500862.1"
                     /translation="MNQWDQFLTPYKQAVDELKVKLKGMRKQYEVGEQASPIEFVTGR
                     VKPIASIIDKANKRQIPFDRLREEMYDIAGLRMMCQFVEDIDVVVNILRQRKDFKVIE
                     ERDYIRNTKESGYRSYHVIIEYPIETLQGQKFILAEIQIRTLAMNFWATIEHTLRYKY
                     DGAYPDEIQHRLERAAEAAYLLDEEMSEIKDEIQEAQKYYTQKRSKKHEND"
     gene            954..1763
                     /locus_tag="POB55_001903"
     CDS             954..1763
                     /locus_tag="POB55_001903"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HDI6500863.1"
                     /translation="MRYTILTKGDSKSNALKHKMMNYMKDFRMIEDSENPEIVISVGG
                     DGTLLQAFHQYSHMLSKVAFVGVHTGHLGFYADWLPHEVEKLIIEINNSEFQVIEYPL
                     LEIIMRYNDNGYETRYLALNEATMKTENGSTLVVDVNLRGKHFERFRGDGLCVSTPSG
                     STAYNKALGGALIHPSLEAMQITEIASINNRVFRTVGSPLVLPKHHTCLISPVNHDTI
                     RMTIDHVSIKHKNVNSIQYSVANEKVRFARFRPFPFWKRVHDSFISSDEER"
     gene            1760..2614
                     /locus_tag="POB55_001904"
     CDS             1760..2614
                     /locus_tag="POB55_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDI6500864.1"
                     /translation="MKFKYHISQQETVKTFLARHDFSKKTVSAIKNNGALIVNDEPVT
                     VRKQLMPNDILEIHLPREIPSVNLIPYARKLEVLYEDAFIIIVTKPNNQNCTPSREHP
                     HESLIEQVLYHCQENGENINPHIVTRLDRNTTGIVIFAKYGYIHHLFSKVNLKKIYTC
                     LVYGKTHTSGIIEANIRRSKDSIITREVASDGKYAKTSYEVINQNDKYSLCKVHLHTG
                     RTHQIRVHFQHIGHPIVGDSLYDGFHDKIHGQVLQCTQIYFVHPINKNNIYITIDYKQ
                     LLKLFNQL"
     gene            2635..4020
                     /gene="mgtE"
                     /locus_tag="POB55_001905"
     CDS             2635..4020
                     /gene="mgtE"
                     /locus_tag="POB55_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HDI6500865.1"
                     /translation="MSMNTDEKERVQEELYDETLLDQYLENDDIDQFRDEFLALHTYE
                     QSEYFEDTTDENRQKIFQYLSPEEVANFFDQLDIDDDEYELLFDKMNATYASHILEEM
                     SYDNAVDILNELTKPKVASLLTLMNKDDANEIKALLHYDEDTAGGIMTTEYLSLKAHT
                     PVKEALLLVKAQAPDAETIYVIFVVDDDGKLVGVLSLRDLIVAENDAYIEDIMNERVI
                     SVNVADDQEDVAQVMRDYDFMAVPVIDYQEHLLGIITIDDILDVMDEEASEDYSRLAG
                     VSDIDSTNDSIIKTALKRLPWLIILTFLGMITATILGRFEKTLENVALLAAFIPIISG
                     MSGNSGTQSLAVSVRNITTGEINEQSKFRIALREAGSGVLSGVVCSTILFTIIVAIYH
                     QPLLALIVAGSLTCAMTVGTFVGSMIPLLMNKLNIDPAVASGPFITTINDIISMLIYF
                     GLATSFMAYLI"
     gene            4030..5874
                     /locus_tag="POB55_001906"
     CDS             4030..5874
                     /locus_tag="POB55_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter family
                     protein"
                     /protein_id="HDI6500866.1"
                     /translation="MEFLSLVIVVLAAFLTPIIVNRLNINFLPVVVAEILMGIVIGNS
                     FLNIVERDSILNILSTLGFIFLMFLSGLEIDFKAFKKDKRARQGQNNDESSIPGHLNL
                     ALTVFAFIMIISILLAYVFKWLGLVDDVLLMVIIISTISLGVVVPTLKEMNIMRTTIG
                     QFILLVAVLADLVTMILLTVYGAINGQGGSTIWLIGILVVFTAISYILGVQFKRMSFL
                     QKLMDGTTQIGIRAVFALIILLVALAEGVGAENILGAFLAGVVVSLLNPDEEMVEKLD
                     SFGYGFFIPIFFIMVGVDLNIPSLIKEPKLLIIIPILIVAFIVSKLIPVMFIRRWFDM
                     KTTIASAFLLTSTLSLVIAAAKISERLNAISAETSGILILSAVITCVFVPIIFKKLFP
                     VPDEFNRKIEVSLIGKNQLTIPIAQNLTSQLYDVTLYYRKDLSDRRQLSDDITMIEIA
                     DYEQDVLERLGLFDRDIVVCATNDDDINRKVAKLAKAHQVERVICRLESTTDDTELVD
                     SGIEIFSSYLSNKILLKGLIETPNMLNLLSNVETSLYEIQMLNYKYENIQLRNFPFGG
                     DIIFVRIIRNNESIVPHGDTQLRYGDRLIVTGAKEYVDELKQELEFYF"
     gene            6152..6922
                     /gene="fabI"
                     /locus_tag="POB55_001907"
     CDS             6152..6922
                     /gene="fabI"
                     /locus_tag="POB55_001907"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HDI6500867.1"
                     /translation="MLNLENKTYVIMGIANKRSIAFGVAKVLDQLGAKLVFTYRKERS
                     RKELEKLLEQLNQPEAHLYQIDVQSDEEVINGFEQIGKDVGNIDGVYHSIAFANMEDL
                     RGRFSETSREGFLLAQDISSYSLTIVAHEAKKLMPEGGSIVATTYLGGEFAVQNYNVM
                     GVAKASLEANVKYLALDLGPDNIRVNAISAGPIRTLSAKGVGGFNTILKEIEERAPLK
                     RNVDQVEVGKTAAYLLSDLSSGVTGENIHVDSGFHAIK"
     gene            complement(7117..8202)
                     /locus_tag="POB55_001908"
     CDS             complement(7117..8202)
                     /locus_tag="POB55_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDI6500868.1"
                     /translation="MLNKVWFRTGIALIMLFILIKLFMEVHEVFTPIATIIGSVFLPF
                     LISGFLFYICLPFQNSLEKVGFPRWASITTIMLALFAIIGLIVAFVAPIIISNINNLI
                     SQTPALQKEAEQIIKFALAQMDKLPEDVTSRITNMVKSMGDGVTNILSNSLQYITSLI
                     STIFLLIMVPFFLIYMLKDHEKFIPAVAKFFKGERKVFFVDLLTDLNFTLKSYIQGQV
                     TVSVILGIFLYIGYSIIDLPYIPLLVLFAGVANLIPFLGSWLSFAPAAILGIIDSPTT
                     FIWVCIITLIAHQLEGNIITPNVMGKSLSIHPLTIIVVILAAGDLGGFTLVLIAVPLY
                     AVLKTVVSNIFKYRQRIIDKANSNVKD"
BASE COUNT         2906 a         1151 c         1519 g         2690 t
ORIGIN      
        1 tctattaaag gattatctga agtaggacaa atgctcgttg atagtgataa ttatgccttt
       61 gcgtatacat tagatgatgg taaagcgtat gcttatctca ttttcgtaca agaaacatgg
      121 acgatgttgc atgaaaacat gactaaaaaa attattatca atgatgaact agaattgact
      181 gaattccacc aagaacttac ttatatttta gacaacataa aagggaataa taattatggt
      241 aaggaatttg ttgcaaccgt tgaagaaaca ttcgacattg aataaagcgg ggtgaagcac
      301 tatgaatcaa tgggatcagt tcttaacacc ttataagcaa gcggttgatg agttgaaagt
      361 gaaacttaaa ggcatgcgca aacaatatga agttggtgaa caagcgtcgc caatagaatt
      421 tgttactggt cgtgttaaac caatcgctag tattatagat aaggcaaaca aacgacaaat
      481 accatttgat aggttaagag aagaaatgta cgatatcgct ggtttaagaa tgatgtgcca
      541 atttgttgaa gatattgatg ttgtcgtcaa tattttaaga caaagaaaag attttaaagt
      601 aattgaagaa cgagattata ttcgtaacac taaagaaagt ggttaccgct cgtatcatgt
      661 cattattgaa tatccaattg aaacattaca aggccaaaaa tttatattgg ctgagattca
      721 gattcgtaca ttagcaatga atttctgggc aacgattgaa catactttac gatataaata
      781 tgatggtgct tatccggatg aaattcaaca tcgtttggaa agagcggcag aagcagcgta
      841 tttacttgat gaagagatgt ctgaaattaa agatgaaatt caggaagctc aaaaatatta
      901 cacgcaaaaa cgttctaaaa aacatgaaaa tgattaacga ggtgttataa atcatgcgtt
      961 atacaatttt aactaaaggt gactccaagt ctaatgcctt aaagcataaa atgatgaact
     1021 atatgaaaga ttttcgcatg attgaggata gtgaaaatcc tgaaattgtt atttcagttg
     1081 gtggtgatgg tacattacta caagcattcc atcagtatag ccacatgtta tcaaaagtgg
     1141 catttgttgg agttcataca ggtcatttag gattttatgc ggattggtta cctcatgaag
     1201 ttgaaaaatt aatcatcgaa attaataatt cagagtttca ggtcattgaa tatccattgc
     1261 ttgaaattat tatgagatac aacgacaacg gctatgaaac aaggtattta gcattaaatg
     1321 aagcaacgat gaaaactgaa aatggctcaa cacttgttgt ggatgttaac ttaagaggga
     1381 aacactttga gcgatttaga ggcgatggat tatgtgtatc aacaccttcg ggttcaacgg
     1441 cttataacaa agcgctaggt ggcgcactga tacatccttc acttgaagca atgcaaatta
     1501 cagaaattgc ctcaataaat aatcgtgtgt ttagaacggt aggatcacca cttgtattac
     1561 caaagcatca tacatgttta atatcaccag ttaatcatga taccattaga atgacgatag
     1621 atcatgttag tatcaaacat aaaaatgtta attcaataca atacagtgta gcaaatgaaa
     1681 aagtgaggtt tgcacgtttt agaccattcc cattctggaa acgtgtacac gattctttca
     1741 tatcaagtga tgaagaacga tgaaatttaa gtatcatata tcacaacagg aaactgttaa
     1801 aactttttta gcacgacatg atttttctaa gaagacagtg agcgccatta aaaataatgg
     1861 cgctttaatt gttaatgatg aaccagtcac agtgcgtaag caattaatgc caaatgatat
     1921 attagaaatt catttaccac gagaaatacc gagtgttaat ttaatacctt atgctcgtaa
     1981 gctagaagta ttgtatgaag atgcttttat catcatagtt actaaaccaa acaatcaaaa
     2041 ttgtacacct tcgagagaac atcctcatga aagtttaatc gaacaagtac tatatcattg
     2101 tcaggaaaat ggtgaaaata ttaacccaca tattgttacg cgtctagatc gtaatacaac
     2161 tggtattgtg atattcgcta aatatggata tatccatcat ttattttcta aagtaaactt
     2221 gaaaaaaata tatacttgcc ttgtatatgg taaaacccat acatctggta ttattgaagc
     2281 taatattaga cggtcaaagg atagtattat aactagagaa gttgcctcgg atggtaaata
     2341 cgctaaaaca tcttatgaag taataaatca gaatgataaa tacagtttat gcaaagttca
     2401 tttgcatacg ggacgtacac atcaaattcg tgtacatttt caacatattg ggcatccaat
     2461 tgtgggagat tctttgtatg atggttttca tgacaaaatt catggtcaag tactgcaatg
     2521 tacgcaaata tattttgttc atccaatcaa taagaacaat atttatatta caattgatta
     2581 taagcaatta cttaaattat tcaatcaact ctaattcaca cagggggtgt aagtatgtca
     2641 atgaacacag atgaaaaaga gcgtgttcaa gaggaattat atgatgagac attattagat
     2701 caatacttag aaaatgatga tattgatcaa tttagagatg aatttctagc attacacaca
     2761 tatgaacaaa gtgagtattt tgaagatact accgatgaaa atagacaaaa gatttttcaa
     2821 tatttatcac ctgaagaggt tgcaaatttc tttgatcaat tagatattga tgacgatgaa
     2881 tatgagttgc tatttgataa aatgaatgcg acatacgcaa gtcacatatt agaagaaatg
     2941 tcatacgata atgcagtaga tattttaaat gagttgacta aaccaaaagt tgctagtctt
     3001 ttaacattga tgaataaaga tgacgcgaat gaaatcaaag cattacttca ctatgatgag
     3061 gatacggccg gcggtattat gacgacggag tatttatcac ttaaagcgca tacgcctgtt
     3121 aaagaagcat tattattggt caaagcgcaa gcaccagacg cagaaacaat atatgttata
     3181 tttgtcgttg atgatgatgg taaattagta ggtgttttat cgctaagaga tttaattgta
     3241 gctgaaaatg atgcttatat tgaagatatt atgaatgaac gtgtcattag tgtgaatgta
     3301 gcagacgacc aagaagatgt tgctcaagtt atgagagact atgatttcat ggctgtacct
     3361 gttatagatt accaagaaca tttgcttggt atcatcacga ttgatgatat tttagacgtt
     3421 atggatgaag aggctagtga agactactct cgtttagccg gggtatcaga tatcgattcg
     3481 actaatgatt caatcattaa aacagcatta aaacgtttac catggttgat tattttaaca
     3541 tttttaggaa tgattactgc gacaatttta gggagattcg aaaaaacatt agaaaatgta
     3601 gcgctactcg cagcgtttat tcctattatt agtggtatgt caggaaattc aggtacacaa
     3661 tctttagccg tttcagttcg taacattacg acaggggaaa ttaatgagca aagtaaattt
     3721 agaattgcat taagagaagc aggaagtggt gtattatcgg gtgttgtatg ttcaacaata
     3781 ttatttacaa ttattgttgc aatatatcat cagccacttt tagcattaat cgttgcagga
     3841 agtttaactt gtgcgatgac ggtggggacg tttgtaggtt cgatgattcc attattgatg
     3901 aataaattaa atatcgatcc agcagtggct agtggaccat ttattacaac aattaatgat
     3961 attattagta tgttgattta ttttggttta gctacatcat ttatggctta cttaatttaa
     4021 ggaggagtta tggagttttt atctttagtt attgttgttt tagcagcgtt tttaactcca
     4081 ataattgtca atcgattaaa tattaatttc ttgccagttg ttgttgcaga aattttgatg
     4141 gggattgtga ttggaaattc atttctaaat atagtagaaa gggattcaat tctaaatatt
     4201 ttatcaacat taggctttat ctttttaatg tttttaagtg gtttagaaat tgattttaaa
     4261 gcttttaaaa aagataaacg cgcacgtcaa ggacaaaata atgatgaatc ctcaattcca
     4321 gggcatctta atctagcgtt aactgtattt gcatttatta tgattatttc gattctttta
     4381 gcgtatgtat ttaaatggct tggattagtg gatgatgtgt tattaatggt cattatcatt
     4441 tcaactattt ccttaggcgt agttgttcca actttaaaag aaatgaatat tatgagaaca
     4501 actatagggc aatttatcct attagtagca gtacttgcgg acttagtaac tatgatttta
     4561 ttaacggtct atggtgcaat caatggtcaa ggcggcagta caatatggtt aataggtata
     4621 ttagttgttt tcacagcaat ttcatatatt ttaggtgttc aatttaaaag aatgtcattt
     4681 ttacaaaaat tgatggatgg tacgacgcaa atcggtattc gtgcggtatt tgcattaata
     4741 atattattag tagccctagc agagggagtt ggcgcagaaa atatattagg tgcattctta
     4801 gcaggtgtcg ttgtttcatt attaaatcca gatgaagaaa tggttgaaaa gttagactca
     4861 tttggttatg ggttctttat tcctattttc tttataatgg ttggtgtaga tttaaacata
     4921 ccttcattaa ttaaagaacc gaaattacta attatcatac cgattttaat cgttgcattt
     4981 atcgtttcaa aattaattcc agtcatgttt attcgacgtt ggtttgatat gaaaacaacg
     5041 attgcatcag catttttatt aacatcaaca ttatcgctcg tgatagctgc agccaaaatt
     5101 tcagaaagat taaatgctat ttcagctgaa acgtcaggta tattaatttt aagcgcagtc
     5161 attacatgtg tattcgttcc gattattttc aaaaaactgt ttccagttcc agatgagttt
     5221 aaccgtaaaa ttgaagttag tttaattggt aaaaatcaat taacgattcc tatagcgcaa
     5281 aatttaacat ctcagttata tgacgtgaca ttatattatc gcaaagactt gagtgatcgt
     5341 cgtcaattgt cggatgatat cacgatgata gaaattgctg attatgaaca agatgtttta
     5401 gaacgactag gtctgtttga ccgagacata gttgtttgtg ccacgaatga cgatgatatt
     5461 aaccgaaaag ttgctaaatt agccaaagca catcaagttg agcgtgtcat ttgcagactt
     5521 gaaagcacaa cggacgatac agagttagtt gattcaggta ttgaaatttt cagtagctac
     5581 ttaagtaata aaatcttatt aaaaggttta attgaaacac ctaacatgtt gaatttatta
     5641 agtaatgttg aaacgtcact atatgaaatt caaatgttaa attataaata tgaaaatatt
     5701 caattacgta atttcccatt cggaggagac atcatcttcg tgcgtattat ccgtaataat
     5761 gagtcgattg ttccgcatgg agatacacaa ttgcgatatg gagatcgctt aattgttacc
     5821 ggtgctaaag aatacgttga tgaattgaag caagagttag aattttattt ttaacaataa
     5881 tgatattaaa atgcagacgt attttagtac gacgtaaaat taatgatatt aaaatgctag
     5941 tatgtatatg attttgataa ataaatgctt tttacgtaaa tcaagtttga tacagaaagg
     6001 actaaatcaa acatttatcg ttgtaatacg tttaaataac tttattaaaa agtcataata
     6061 gtgttaaaat gtattgacga ataaaaagtt agttaaaact gggattagat attctatccg
     6121 ttaaattaat tattataagg agttatctta catgttaaat cttgaaaaca aaacatatgt
     6181 cattatggga atcgctaata agcgtagtat tgcttttggt gtcgctaaag ttttagatca
     6241 attaggtgct aaattagtat ttacttaccg taaagaacgt agccgtaaag agcttgaaaa
     6301 attattagaa caattaaatc aaccagaagc gcacttatat caaattgatg ttcaaagcga
     6361 tgaagaggtt attaatggtt ttgagcaaat tggtaaagat gttggcaata ttgatggtgt
     6421 atatcattca atcgcatttg ctaatatgga agacttacgc ggacgctttt ctgaaacttc
     6481 acgtgaaggt ttcttgttag ctcaagacat tagttcttac tcactaacta tcgttgctca
     6541 tgaagctaaa aaattaatgc cagaaggtgg tagcattgtt gcaacaacat atttaggtgg
     6601 cgaattcgca gttcaaaact ataatgtgat gggtgttgct aaagcgagct tagaagcaaa
     6661 tgttaaatat ttagcattag acttaggtcc agataatatt cgcgttaatg caatttcagc
     6721 tggtccaatc cgtacattaa gtgcaaaagg tgtgggtggt ttcaatacaa ttcttaaaga
     6781 aatcgaagag cgtgcacctt taaaacgtaa tgttgatcaa gtagaagtag gtaaaactgc
     6841 ggcttactta ttaagtgatt tatcaagtgg cgttacaggt gaaaatattc atgtagatag
     6901 cggattccac gcaattaaat aatatcattc aacagctttg ttcacgttat tatatatgtg
     6961 agcaaagctt ttttgctttt ataataatcg gactgatgga aaattatttg atatttcatt
     7021 tgactgattt tttatgcaca aaaaaatcgc ctcaaaagct gtagtttcaa cttttgatgc
     7081 gatagtatct tatattctta aaagacatcc acagaattag tccttaacat tactgtttgc
     7141 tttatcaata atgcgttggc ggtatttgaa aatattacta acaaccgttt taagtacagc
     7201 atataatggc actgcaatca gaactaatgt aaagccacct aaatctcctg ctgctaaaat
     7261 aacaacgata attgttagag gatggatact taaagattta cccattacat ttggcgtaat
     7321 gatattacct tcaagttggt gtgcaattaa tgtaatgata caaacccata taaatgtagt
     7381 aggactatct ataataccga gtattgctgc aggtgcaaat gataaccatg aacctaagaa
     7441 aggaattaag tttgcgacac cagcaaatag tactaataaa ggaatatatg gtaagtcaat
     7501 aattgaataa ccgatatata agaatatacc taaaataaca ctgacagtta cttgaccttg
     7561 aatgtaagat tttaatgtaa agtttaaatc agttaataaa tctacgaaaa atactttacg
     7621 ttcacctttg aaaaatttag caacagctgg gataaatttt tcatggtctt ttaacatata
     7681 aattaagaag aatggaacca taatcaataa gaagatggtt gaaattaatg atgtaatgta
     7741 ctgtaatgaa ttagataaaa tattagtaac gccatcaccc attgatttaa ccatatttgt
     7801 aattctactt gttacatctt caggtaattt atccatttga gctagtgcga atttaataat
     7861 ttgctctgct tctttttgta gggcaggtgt ctgactaatt aaattgttga tatttgaaat
     7921 gatgattggt gcaacaaacg caacaattaa gccgataata gcaaacaaag ctaacatgat
     7981 tgttgttata ctagcccatc ttggaaaacc gactttttca agtgagtttt gaaaaggtag
     8041 acagatataa aataaaaaac cactaattaa aaatggaaga aatacagaac cgatgattgt
     8101 agctattgga gtaaatactt cgtgcacttc cataaatagt ttgatgagaa tgaacagcat
     8161 aatcagagcg atgccagttc ggaaccaaac cttgtttaac ataaacgatg ttcctcctat
     8221 ttttctaaac ttagttatta tttagtatat cctaaatagc agtgct
//
LOCUS       DAMZKC010000113         8054 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.114, whole genome shotgun sequence.
ACCESSION   DAMZKC010000113 DAMZKC010000000
VERSION     DAMZKC010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8054
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.114"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            137..760
                     /locus_tag="POB55_001909"
     CDS             137..760
                     /locus_tag="POB55_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDI6500869.1"
                     /translation="MKEDRRIRKTKSSIKQAFTKLLQEKDLEKITIRDITTRADINRG
                     TFYLHYEDKYMLLADMEDEYISELTTYTQFDLLRGSSIEDIANTFVNNILKNIFQHIH
                     DNLEFYHTILQLERTSQLELKINEHIKNNMQRYISINHSIGGVPEMYFYSYVSGATIS
                     IIKYWVMDKQPISVDELAKHVHNIIFNGPLRIMAENRLHKSNLDSLT"
     gene            806..1750
                     /locus_tag="POB55_001910"
     CDS             806..1750
                     /locus_tag="POB55_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDI6500870.1"
                     /translation="MITSFRHSEDIDKHIIKTPLDHTASWINVVEPDREEIENLMEQY
                     NIPEDFIRDPLDSEESSRIEYDEDTGYSLIIIDLPIVNSTNRSVLSFVTIPLGIIIGN
                     GIIVTVCDAENEFLENLPKRDINLKFHSRFALEILTTIADHYNRNLRLLNKSRIRIEK
                     ELKNNITNKQLFKLMEVEKSLVYFLAALKGNDTIIKKLFRLPAIKRFEEDEELLEDLI
                     IENNQAIETTELHQRILESITTSYASLLSNDMNTIMKTLTLFTVLLTLPTLVFSFFGM
                     NVPLPIDDHSYISWIIVVGISLILVVIVSIFLWRKQKL"
     gene            complement(1835..3277)
                     /locus_tag="POB55_001911"
     CDS             complement(1835..3277)
                     /locus_tag="POB55_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sucrose transporter subunit IIBC"
                     /protein_id="HDI6500871.1"
                     /translation="MNYKQSAEEILNAIGGEENLDAMAHCATRLRLVLNDESLVNEEA
                     LNNMDVVKGTFSTGGQYQIIIGSGTVNKVFSELEKLTGKEASTTSEVKAQSAKNMNPL
                     QRFVKMLSDIFVPIIPAIVAGGLLMGLNNILTAKDLFFSGKSLIDVYSQFAGLAEMIN
                     VFANAPFTLLPILIGFSAAKRFGGNPFLGAALGMILVHPSLMSAYDFPKAVEAGKAIP
                     YWDVFGLHINQVGYQGQVLPMLVAAYILASIEKGLRKVIPTVLDNLLTPLLSIFITAF
                     LTFSFVGPITRQLGYWLSDGLTWLYEFGGAIGGLIFGLLYAPIVITGMHHSFIAVETT
                     LIADATKTGGSFIFPIATMSNVAQGGAAIAAFFIIKQNKKLKGVASAAGISALLGITE
                     PAMFGVNLKLRYPFIGAIVGSGIGSAYIAFFKVKAIALGTAGLPGFISINPVHAGWLH
                     YFVGMTISFIIAITVTLILSKRKANKEVVE"
     gene            3460..3843
                     /locus_tag="POB55_001912"
     CDS             3460..3843
                     /locus_tag="POB55_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HDI6500872.1"
                     /translation="MKERGYIEQLWREEKYHVLLHSQQSYQMIRNALKTDLSLHQVQQ
                     MIDDALLIEPSIGSVCNAFDHMWGYFKKCANEEERQQSKLLKADFINGKIDTQTLLDF
                     LAELANKYDVQYLLQSRVLNTKRKR"
     gene            5331..7436
                     /gene="rsp"
                     /locus_tag="POB55_001913"
     CDS             5331..7436
                     /gene="rsp"
                     /locus_tag="POB55_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator Rsp"
                     /protein_id="HDI6500873.1"
                     /translation="MTCQLKIHHTLSTIPSRNINNIMVLFSLTSKLNITINGETKDVS
                     NYIILINHGDIYNINHGENIIELMIPVFYFYQQDDDFFNGYLDRHLLQSSNYIKSLIA
                     DLISTPTRSSLMGKNIGQSIIDTLLKEAFIRIDHEYLPNIALSNPVFIDCVNYIHDNI
                     DAHLSLKDIAMHCNISESYCSNLFVRYLSMNFKDYFTSIKLVNAINLLLSTKHSITTV
                     SELAGFNSHTNFANQFKNYLHFSPKQFRSLVSKITEPPQIHFQQDNVSQFTELISTID
                     LTAQLATNTTDIHIDDFNPKDRSQRAKVFVRFSNFNELFQFIFNEYYDINFEHLPKPV
                     VFIDDIHDIEISQTNYNLLNRCFEKLFEKNIGLAIAIKSTQQFETMKQLILTFLQGNQ
                     DYKTSKKLVKFMLVFCSNSMTAEEIHLCHLKIKNKNKEIKYSVTVDGFLETYSTVEQV
                     YDVMQRLKFHYYFIDIENSKTATHLITKNQHYHQTDTHFEQYKKFILDSGISSTQFVY
                     NNLSVSGFKYTNDGKNPIQLSDIVYHLIALLRYGGGISYQLLDDHSNYISLYNKYGSP
                     LPLMHLYKMFRPFVNEDIEITNNYVLSRKDNNYHFLLFNKINDRYMSDVKQDFIFHNE
                     LPQDSLMIIKTLNHEHGSIQHLLPISDQLVYIEKEILDELDKTNYPKTELAVQEETGR
                     TFELKLNHDEVKYICFKPS"
     gene            7507..7929
                     /locus_tag="POB55_001914"
     CDS             7507..7929
                     /locus_tag="POB55_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HDI6500874.1"
                     /translation="MSNSQAIQAIENVLATSKVGVLSTAYNNKPNSRYMVFYNDGLTL
                     YTKTNIHSAKVKEIKDNPAAYVLLGYNDTTNRSFVEMEATIEVVTDQKVIDWLWETQD
                     KSFFSSKEDPELCVLKVTPQSVKLMNDKSLDTPIKIDL"
BASE COUNT         2836 a         1248 c         1172 g         2798 t
ORIGIN      
        1 caatgtgttg aatatttcgc atgttgtcta ttataatatg taagtgggaa taaacaatac
       61 acaatgttca tcaatacgtt tattaataga caaatacacc cgcattgttc aataacacga
      121 ggaggttgat ttatttttga aagaagatag gcgaattaga aagacaaagt cctccattaa
      181 acaagcgttc actaaattac tacaagaaaa agatttagaa aagattacca ttcgcgacat
      241 aacaacacgc gctgatatca atagagggac attttactta cattacgagg ataaatatat
      301 gttactcgca gatatggaag atgagtatat ttcagaacta acgacatata ctcaatttga
      361 tttgttacgc ggttcttcaa ttgaagacat tgcgaatact tttgtaaata atatactcaa
      421 aaatattttc caacatattc atgataattt agagttttat catactattt tacaattgga
      481 acgcacgagt caattagaat tgaaaatcaa cgaacatatt aaaaataata tgcaacgtta
      541 tattagtatc aatcattcca tcggaggcgt tccagagatg tatttttaca gttacgtttc
      601 cggagcaaca atttcaatta ttaaatactg ggtaatggac aaacagccca tttcagttga
      661 tgaattagct aaacatgtgc ataatattat ttttaatggt ccattaagaa taatggcaga
      721 aaaccgtttg cataaatcaa accttgattc actaacttaa gtaatactcg cttttcctaa
      781 atcctttaga aaggaactat atatcatgat tacttcattc agacattctg aagatataga
      841 taaacatatt ataaaaacac cattagatca tacagcgtca tggattaatg tagtagaacc
      901 agaccgagaa gaaattgaaa atcttatgga acaatataat atacctgaag actttatacg
      961 tgaccctttg gactcagaag aaagttcccg tattgaatat gacgaagata ctggttactc
     1021 attaatcatt attgatttac ctatcgtcaa ttcaactaat cgtagcgttc tatcttttgt
     1081 aacgattcca ttaggcatta ttattggcaa cggtattatc gtaacagttt gtgacgctga
     1141 aaatgaattt ttagaaaatt tacctaagcg tgatattaat ttaaaatttc acagcagatt
     1201 tgcattagaa attttaacta ctattgccga ccattataat cgtaacttac gattacttaa
     1261 taaaagtaga attcgtattg agaaagaact aaaaaataac attactaaca aacaactttt
     1321 caaattaatg gaagttgaaa aaagtttagt atacttttta gctgccttaa aaggtaacga
     1381 tacaattatt aaaaagttat tccgtttacc tgcaattaaa cgttttgaag aagatgaaga
     1441 attactagaa gatttaatca ttgaaaataa ccaagccatc gaaacaacag aattacatca
     1501 acgtatctta gaaagtatca caacatcata cgcttcctta ttatctaatg atatgaatac
     1561 gattatgaag acattaacac ttttcacggt actattaacg ttaccaacac tcgtatttag
     1621 tttcttcggt atgaatgtgc cgttgccaat tgatgaccat agttatatat cttggattat
     1681 tgtggtaggt atttcattaa ttcttgtcgt aattgttagt attttcttat ggcgtaaaca
     1741 gaaattataa ctaaattaaa aggtatgaca gcttaaagtt tcatcctatt gcatttgcaa
     1801 tgactgatac tttgattact gtcatacctc ttttttattc tacaacttct ttatttgctt
     1861 ttcttttaga taaaattaaa gtaactgtta tagcaatgat gaatgatatt gtcataccaa
     1921 caaagtagtg taaccatcct gcatgtactg gattgattga aataaatcct ggcaatccag
     1981 cagttcctaa tgcgattgct ttaaccttga agaaagcaat atatgctgaa ccaatacctg
     2041 atccaacgat agcgccaata aatggatatc ttagttttaa gttaacacca aacatagccg
     2101 gttctgtaat accaagtaat gctgaaatac ctgcggcaga tgccacacct tttaacttct
     2161 tattttgttt aataataaag aacgctgcaa ttgctgcacc accttgtgca acattagaca
     2221 ttgtcgcaat cgggaatata aatgatccac ccgttttagt ggcatctgca attaatgtcg
     2281 tttctacagc tataaagcta tgatgcatac ctgtaataac aatcggagca tacaataatc
     2341 cgaatattaa tccaccaatt gcaccaccaa attcataaag ccatgttaaa ccatctgata
     2401 accagtaacc taattgtcga gtgattggac ctacaaatga aaatgttaga aatgctgtta
     2461 taaaaataga taacaatggt gttaacaaat tatctaacac cgttggaata actttgcgta
     2521 accctttttc aattgaggct aagatataag ctgctacaag cataggtaac acttgtcctt
     2581 gataacctac ttgattaata tgcaaaccaa aaacatccca atatggaata gccttacctg
     2641 cttcaactgc ttttgggaaa tcgtatgcgc tcattagcga tggatgaact agtatcatac
     2701 ctaatgcagc acctaaaaat ggattgccac caaagcgttt tgctgcacta aatccaatta
     2761 aaattggtaa taatgtaaat ggtgcattcg caaaaacatt tatcatttca gctaatccag
     2821 caaattgact atatacatct atcaatgatt tacctgaaaa gaataaatct ttcgcagtca
     2881 aaatgttatt taaccccatt aataaaccac cagcaacgat ggctggtata atcggaacaa
     2941 agatatctga aagcattttt acaaatcgct gtaacggatt catattttta gcagattgtg
     3001 ctttgacttc cgaagtggtt gatgcttctt ttccagttaa tttttccagt tcactaaata
     3061 ctttattgac tgtaccagac ccaataataa tttggtattg tcccccagta gaaaacgtcc
     3121 ctttaactac atccatattg tttagcgcct cttcatttac taaactttca tcatttaaaa
     3181 ctaatcgtag tctcgttgca caatgtgcca ttgcatctaa attctcttct ccgcctatcg
     3241 cgttcaaaat ttcttctgcg gattgcttat aattcatata tttagcctcc taattagaac
     3301 cggttccatt tattccactt tattgtaacc ggttccaaaa atatttgcaa cactctatta
     3361 tcattttata taaacaccta caccattttt gatagcgcat tcatttttat tgctatacta
     3421 catgcatgta tattaacatt aattatcgga ggtaatttta tgaaagaacg tggctatatc
     3481 gaacagcttt ggcgtgagga aaaatatcat gtattgttac acagtcaaca gtcttaccaa
     3541 atgataagga atgctttaaa aacggattta tcacttcatc aagttcaaca aatgattgat
     3601 gatgctttgt taattgaacc gagtatagga agtgtatgta atgcttttga tcatatgtgg
     3661 ggatatttta aaaaatgtgc gaatgaagaa gaaagacaac aatcaaaact acttaaagct
     3721 gattttatta atggtaaaat cgacacccaa actttattag attttttagc agaacttgct
     3781 aataaatatg atgttcaata tttactacaa agtcgtgttt taaacacaaa aagaaagaga
     3841 tagattgaaa ctttagttgt aacggacaaa tagtagtcat tggagttcag caattcatac
     3901 tttgtactaa agtttactat aatctatctc tcaaggttta aatgtacata tctatattaa
     3961 aatgcgtttc atgatgttag aagataccat acacatttaa ttagttacag cataaatcaa
     4021 tttataccct taattattac attgtttata ttttaaattc gggggagtaa ttttaagtaa
     4081 tatcttgttg ctgctaattc tattgttaat aaattgatat tagatgtaga aattgagtgt
     4141 gaaagttaat aatagatgat ttagctagta acataattaa ttcatatata gtcaaaaagt
     4201 tctgttagat actgttcttg atgaatattc tttgatgtta ggtgttagct attctagatg
     4261 agtttcttag atgtggatgc ctgtcttgat gaagttatct atgatgttaa tataatactt
     4321 gatgttgatt cttagatgtt agtgcgattt caagatgctg attctttgtt gttgcactat
     4381 cttggtgacg tttctagttg tttatagtta tcgcatctct tgatgtaaaa tctaagatgt
     4441 ttatgcattt ctagatgtta attcttcgct gcttaagaat tatcttgatg gtaaatctag
     4501 ttgtgccatg cattttctca tgatgaatct aaggtgttaa ttcattgatg tgtgctgttt
     4561 cttgttgctt cattatcttg atggtgaatt tcgttgtcta atgcactttt tcaaatgatt
     4621 attctaattg ttacggcaat aactagatgg attttcttag atgttcactt ttctagatgt
     4681 ttatacttat cttgatgttt tctaaacaga acttttaaac gctttatttt ttagtagatt
     4741 gtttaaaatt tcttagttga tggttattgt tgttgatttg aataatttgt agcgaatatt
     4801 ttgtaacagg gctactaaga atttttataa caagaagtga tttcaatcgt actaattttc
     4861 agcgtttgat gacaaaagtg tatgtccaaa cttaaataat ggcgataaaa tttaagtcat
     4921 gggtacattt aatgtttttt gattcaaact tccattttgg tcaatttgct ttttttataa
     4981 catagttgaa tatcaataaa agattagcaa gttagttcaa aatggctatg tattgttttt
     5041 tgaaatacat aggtttgaaa tctatattaa ttatgcacca aataatttaa ttagactcta
     5101 tcgaaaattt ccaaattttg cttactatct ttctaatttt acttttatat tgtgcacaat
     5161 gcataaaaac aagcgttttc ctaggagttt tcattaattt cttttatttt tcactattac
     5221 cttttcaaaa atattcaact ataataggta tactatccat gattttaaaa atttccatgt
     5281 tttacattta aatgatagat ttcaatatat attaggcagg agagagaaat atgacatgcc
     5341 aacttaaaat acatcataca ttatcgacaa tacccagtcg taatattaat aatattatgg
     5401 tgctattttc acttacaagt aaactcaata ttacgattaa tggtgaaacc aaagacgtaa
     5461 gtaattatat tatcttaatt aaccatggtg atatttataa tattaaccac ggcgaaaata
     5521 taatcgaatt aatgataccc gttttttatt tttatcaaca agatgatgat ttctttaatg
     5581 gttatttaga tcgtcattta ttacaatcaa gtaattatat taagtcatta attgccgatt
     5641 taatcagtac accaacaaga tcttctttaa tggggaaaaa tataggtcaa agtattatag
     5701 acactttact taaagaagct tttataagaa tagatcatga atatttacca aatattgctt
     5761 taagcaatcc ggtgttcata gactgtgtaa actatattca tgacaatatt gatgcgcact
     5821 tgtctttaaa agatatagcg atgcattgca acatctctga atcatattgt tctaatcttt
     5881 ttgttcgata tttgagtatg aattttaaag attattttac aagtattaaa cttgttaacg
     5941 ccatcaattt attactttct acaaaacatt ctattacaac tgtttctgaa ttggccggtt
     6001 ttaacagtca tacaaatttt gcaaatcaat ttaagaatta cttgcatttt agtcctaaac
     6061 agttccgttc actcgtttcc aagattactg aaccaccaca aatacacttc caacaagata
     6121 acgtatcaca attcactgag ttaatttcaa caattgattt aacagctcaa ctggctacaa
     6181 atacaacaga tattcatata gatgatttta atccgaaaga tcgaagtcag cgtgccaaag
     6241 tatttgttcg atttagtaat ttcaatgaac tatttcaatt tattttcaat gaatattacg
     6301 atattaactt tgagcactta ccaaaacctg tcgttttcat tgacgatatt cacgatattg
     6361 agattagcca aacgaattac aacctgttaa atagatgttt tgaaaagctt ttcgaaaaaa
     6421 atataggttt agcaatcgct attaaatcaa cacaacaatt cgaaacgatg aagcaattaa
     6481 tactcacatt tttacaaggc aatcaagact ataagacaag caagaaatta gttaaattta
     6541 tgctcgtatt ttgctctaat tctatgacag cagaagaaat tcatttatgt catcttaaaa
     6601 ttaaaaacaa aaataaagaa attaaatata gtgtgacagt cgatggcttt ttagaaactt
     6661 attcaaccgt ggaacaagtt tatgatgtca tgcagcgtct taaattccat tattacttta
     6721 ttgatattga aaattcaaaa actgcaacac atcttatcac taaaaaccaa cactatcatc
     6781 aaactgacac tcattttgaa cagtataaga aatttatttt agattcaggt atatcatcaa
     6841 cacaatttgt atataataac ctgtctgtaa gcggatttaa atatactaat gatggtaaga
     6901 atccaattca attatctgac atagtgtatc acttaattgc attattacga tatggcggtg
     6961 gcattagcta tcaattatta gatgaccatt caaattatat ttccttgtac aacaaatatg
     7021 gtagtcccct accattaatg catttatata aaatgtttag accttttgtt aatgaagata
     7081 ttgaaattac aaataattat gtattgagtc gtaaagataa taactaccat ttcttattat
     7141 tcaataaaat taatgatcga tatatgtcag acgtaaaaca agatttcatt ttccataatg
     7201 aattacctca agactctttg atgattatta aaacattgaa tcatgaacat ggttcaattc
     7261 aacatttgct tccaataagc gatcaacttg tttatataga aaaagaaatt ttagatgaat
     7321 tagacaaaac gaattaccct aaaacggagc ttgcagttca agaagaaact ggtcgtacat
     7381 ttgaactcaa gttaaatcac gacgaggtta aatatatttg ctttaaacca agctaaatac
     7441 taacagtcct cttgtgttta gtttcttacg ttaaaggcta tttatatcat aaggagatga
     7501 tatgtaatga gtaactcaca agcaattcaa gcaattgaaa acgtgttagc aacgtcaaaa
     7561 gttggtgtat tatcaactgc atataataat aaacctaata gtagatatat ggtcttttat
     7621 aatgatggtc ttactttata tactaaaacg aatatccatt ctgctaaggt caaagaaatt
     7681 aaagataatc cagcagcata tgttttgtta ggctacaatg atacgacaaa tcgcagtttc
     7741 gttgaaatgg aagccacgat agaagtagtt actgatcaaa aagtaatcga ttggttatgg
     7801 gagactcaag ataaaagctt tttcagttca aaagaagatc cagaactttg tgttttaaag
     7861 gtgacaccac aatctgttaa attgatgaat gacaaatcat tagatacacc tatcaaaatc
     7921 gatttataac acaaagtata tataggaaat aacttttatg aattctagat ataacaatgt
     7981 taaatactta aaataactcg ctataattaa agtgtttaat atgtttacaa ttcaatttgc
     8041 aatgtattgt tttt
//
LOCUS       DAMZKC010000114         7875 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.115, whole genome shotgun sequence.
ACCESSION   DAMZKC010000114 DAMZKC010000000
VERSION     DAMZKC010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7875
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.115"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..580
                     /locus_tag="POB55_001915"
     CDS             <1..580
                     /locus_tag="POB55_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDI6500875.1"
                     /translation="EYVASIVSYIIEYTTITSSKCLVLFTSYKMMHMVQDMLNELPEF
                     EDYVVLTQQQNQNYKIVQQFNNFDKAILLGTSTFFEGFDFQANGIKCVMIAKLPFMNK
                     HNAKYWLMDSEFTSTFKEYVLPDAVTRFRQGLGRLIRSENDRGIIVSFDDRLINSNYK
                     NFFEQTLENYRQKKGDIQQFGKLLRQIQKKKK"
     gene            902..2194
                     /gene="asnS"
                     /locus_tag="POB55_001916"
     CDS             902..2194
                     /gene="asnS"
                     /locus_tag="POB55_001916"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HDI6500876.1"
                     /translation="MKTTIKQAKDHLNQDVTIGAWLTNKRSSGKIAFLQLRDGTGFMQ
                     GVVVKSEVDEEVFKLAKEITQESSLYVTGTITEDNRSDLGYEMQVKSIEVISEAHDYP
                     ITPKNHGTEFLMDHRHLWLRSKKQHAVMKIRNEVIRATYEFFNKDGFTKVDPPILTAS
                     APEGTSELFHTKYFDQDVFLSQSGQLYLEAAAMAHGKVFSFGPTFRAEKSKTRRHLIE
                     FWMIEGEMAFINHAESLEIQEQYVTHVVKSVLENCKLELKILERDTSKLEKVATPFPR
                     ISYDDAIEFLKSEGFDDIEWGEDFGAPHETAIANHYDLPVFITNYPTKIKPFYMQPNP
                     ENEETVLCADLIAPEGYGEIIGGSERVDDLELLEQRVKEHGLDEEAYSYYLDLRRYGS
                     VPHCGFGLGLERTVAWISGVEHVRETAPFPRLLNRLYP"
     gene            2522..3208
                     /locus_tag="POB55_001917"
     CDS             2522..3208
                     /locus_tag="POB55_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HDI6500877.1"
                     /translation="MDKYQLKARPVVIRRELLDHYSDLGLDEQDLVILLKLIYASETS
                     NKQPSIELLQKGSTMQPRDITMVIQNLIQRELLELQVQKDEEGRFTEYMNLDPFFEKL
                     SHILKQQSMETKEQNSKEKFKQLFRLLEDTFARPLSPYEIETLNQWIDVDKHDSAIIQ
                     AALDEANSLNKLSFKYMDRILLNWKKNNVKTIDDSRKIREKFNKPKMTHTVKTVPKFD
                     WLNGENLDGK"
     gene            3198..3857
                     /gene="nth"
                     /locus_tag="POB55_001918"
     CDS             3198..3857
                     /gene="nth"
                     /locus_tag="POB55_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HDI6500878.1"
                     /translation="MVSKKKALEMIDVIANMFPDAECELKHDNPFELTIAVLLSAQCT
                     DVLVNRVTTELFKKYKTPEDYLAVSDEELMNDIRSIGLYRNKAKNIKKLCQSLIDQFN
                     GEIPQTHKELESLAGVGRKTANVVMSVAFDEPSLAVDTHVERVSKRLGINRWKDNVRQ
                     VEDRLCSVIPRDRWNSSHHQLIFFGRYHCLARKPKCDICPLLEDCREGQKRYKASLKE
                     A"
     gene            3862..4203
                     /locus_tag="POB55_001919"
     CDS             3862..4203
                     /locus_tag="POB55_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500879.1"
                     /translation="MTNITKEVFDNLEQEIDVFAKNKTLGSSEAKPYLDEYHSKIIDY
                     FKQVNDITGNIDFDKLNQYPVVPMNFKERYDYMIERKYHFMGYRQMKTFKTELIKMNA
                     SYQTRLKNKQV"
     gene            complement(4767..6950)
                     /locus_tag="POB55_001920"
     CDS             complement(4767..6950)
                     /locus_tag="POB55_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDI6500880.1"
                     /translation="MTENKGSSQPKKNGNNGGKSNSKKNRNVKRTIIKIIGFMIIAFF
                     VVLLLGILLFAYYAWKAPAFTEAKLQDPIPAKIYDKNGELVKTLDNGQRHEHVNLKDV
                     PKSMKDAVLATEDNRFYEHGALDYKRLFGAIGKNLTGGFGSEGASTLTQQVVKDAFLS
                     QHKSIGRKAQEAYLSYRLEQEYSKDDIFQVYLNKIYYSDGVTGIKAAAKYYFNKDLKD
                     LNLAEEAYLAGLPQVPNNYNIYDHPKAAEDRKNTVLYLMHYHKRITDKQWEDAKKIDL
                     KANLVNRTAEERQNIDTNQDSEYNSYVNFVKSELMNNKAFKDENLGNVLQSGIKIYTN
                     MDKDVQKTLQNDVDNGSFYKNKDQQVGATILDSKTGGLVAISGGRDFKDVVNRNQATD
                     PHPTGSSLKPFLAYGPAIENMKWATNHAIQDESSYQVDGSTFRNYDTKSHGTVSIYDA
                     LRQSFNIPALKAWQSVKQNAGNDAPKKFAAKLGLNYEGDIGPSEVLGGSASEFSPTQL
                     ASAFAAIANGGTYNNAHSIQKVVTRDGETIEYDHTSHKAMSDYTAYMLAEMLKGTFKP
                     YGSAYGHGVSGVNMGAKTGTGTYGAETYSQYNLPDNAAKDVWINGFTPQYTMSVWMGF
                     SKVKQYGENSFVGHSQQEYPQFLYENVMSKISSRDGEDFKRPSSVSGSIPSINVSGSQ
                     DNNTTNRSTHGGSDTSANSSGTAQSNNNTRSQQSRNSGGLTGIFN"
     gene            complement(6947..7624)
                     /gene="recU"
                     /locus_tag="POB55_001921"
     CDS             complement(6947..7624)
                     /gene="recU"
                     /locus_tag="POB55_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HDI6500881.1"
                     /translation="MLCIINNVYYQTCVVKFMNYPNGKPYRKNSAIDGGKKTAAFSNI
                     EYGGRGMSLEKDIEHSNTFYLKSDIAVIHKKPTPVQIVNVNYPKRSKAVINEAYFRTP
                     STTDYNGVYQGYYIDFEAKETKNKTSFPLNNIHDHQVEHMKNAYQQKGIVFLMIRFKT
                     LDEVYLLPYSKFEVFWKRYKDNIKKSITVDEIRKNGYHIPYQYQPRLDYLKAVDKLIL
                     DESEDRV"
BASE COUNT         2732 a         1180 c         1342 g         2621 t
ORIGIN      
        1 tgagtatgta gcatcgattg taagctatat tattgaatat acgactataa cgtcatcaaa
       61 atgtttagtc ttatttacga gttataaaat gatgcatatg gtacaagata tgctaaatga
      121 attgccagaa tttgaagatt atgttgtatt aacacaacag cagaaccaaa attataaaat
      181 agtacaacag tttaataatt ttgataaggc catactgctt ggtacgtcaa cattttttga
      241 aggttttgat tttcaagcaa atggtattaa atgtgtgatg atagcgaagt taccgtttat
      301 gaacaagcat aatgcaaaat attggcttat ggattctgaa ttcacttcaa ctttcaagga
      361 atatgtatta cctgatgcag ttacacgttt tagacaagga cttggaagat taattagaag
      421 tgaaaatgat cgcggcataa tagtttcatt tgatgatcga cttataaaca gtaattataa
      481 aaactttttt gaacaaacac ttgaaaatta tcgtcagaaa aaaggcgaca ttcaacagtt
      541 tggaaaatta ttaagacaaa ttcaaaaaaa gaaaaagtga aatacaaagt aaaattcttg
      601 tgacatgaca tgtgctatgt tctgttacaa gaatttttgt ttggtcgtca aaatttgagt
      661 tttataatta aacttgaatg ttagcacaaa aaacaacctt catactaata atattgaggc
      721 tttattatat attttgtctg aaactgatga ttgtattgtc ttaatataaa ttaatgttca
      781 aatctgtgct taaatcatta ccatttacac gttatctttt agtctagttc tagaacttga
      841 aagcggattt tgttaaaatg aatgagtatt gtataacgaa aaaataaata ggagaatggt
      901 tatgaaaaca acgattaaac aagcgaaaga tcatttaaac caagacgtta caattggtgc
      961 ttggctaaca aataaacgtt caagtggtaa aatcgccttt ttacaattac gtgatggaac
     1021 aggctttatg caaggcgtag tagttaaatc agaagttgat gaagaggtat tcaaacttgc
     1081 gaaagaaatt actcaagaat catctctata cgttacaggc acaattacag aagataatcg
     1141 ttctgactta ggatacgaaa tgcaagtgaa atcaattgaa gttatttcag aagcgcatga
     1201 ctatccgatt acacctaaaa atcatggtac agaattctta atggatcacc gtcatttatg
     1261 gttacgttct aaaaaacaac atgctgtaat gaaaattaga aatgaagtta ttcgtgcaac
     1321 gtatgaattt ttcaacaaag atggatttac aaaggttgat ccaccaattt tgacagcaag
     1381 tgcgccagaa ggtacaagtg aattattcca tactaaatac tttgatcaag atgtgttttt
     1441 atctcaaagt ggtcagttat acttagaagc tgcagcaatg gcacacggaa aagtattttc
     1501 atttggtcca actttcagag ctgaaaaatc aaaaacacgt agacacttga tcgagttctg
     1561 gatgattgaa ggggaaatgg ctttcataaa tcatgctgaa agtttagaaa ttcaagaaca
     1621 atatgtaaca catgttgtaa aatcagtttt agaaaattgt aaactagagt tgaaaatttt
     1681 agagcgtgat acatcaaaac ttgaaaaagt tgcgacacca ttccctagaa tttcatatga
     1741 tgatgcaatt gaattcttaa aatcagaagg ctttgatgat attgaatggg gcgaagattt
     1801 tggtgcgcca catgaaacag ccattgctaa tcattatgat ttaccggtgt ttattactaa
     1861 ttatccaact aaaattaagc ctttctatat gcaaccaaat cctgaaaatg aagaaactgt
     1921 attatgtgca gacttaattg cacctgaagg atacggtgaa attattggtg gatctgaacg
     1981 tgtggatgac ttagaattgt tagaacaacg cgttaaagaa catggattag acgaagaagc
     2041 atatagttac tacttagact tacgtcgtta tggtagtgtg ccacactgtg gatttggttt
     2101 aggtttagaa cgtacagtag catggatttc tggtgttgaa cacgttcgtg aaacagcgcc
     2161 attcccaaga ttattaaacc gtttatatcc ataagtttta gggtcgttac ttaaaatata
     2221 tattatgata ataaaaagaa tcgtccagcc tatattaata gctgggcgat ttatgcttgg
     2281 aaaatgaaga ttgataacgg cttatattaa tcatgaaaaa acttttgttt agtaactata
     2341 tttggaaaat aaatagttca tctgattata aataaagttc attgttaaaa tctagtgtta
     2401 aaaaatactg ttatttaaaa tttgtttgct actagttaaa tatattaagc attttagtcg
     2461 aaaattaaag aaaatgattt atactattaa gtaatggtta aatttaaaag ggggcacgaa
     2521 catggataaa tatcaattaa aagcaagacc tgtagtgata cgaagagaat tattagacca
     2581 ttacagtgac ttaggtttag atgagcaaga tttagtcatt ttgcttaaat tgatttatgc
     2641 ttccgaaaca tcaaataaac agccttcaat tgaattactt cagaagggct caactatgca
     2701 accacgcgat attacaatgg ttattcaaaa tttgatacaa cgtgaattgc tagaattaca
     2761 agttcaaaaa gatgaagaag gtagatttac cgaatatatg aatctagatc cttttttcga
     2821 aaaattaagt catatattga agcaacaaag tatggaaacg aaagagcaaa atagtaaaga
     2881 aaaatttaaa caattattta gacttttaga ggatacattt gcaaggccgt tatctccata
     2941 tgaaatagaa acgttaaatc aatggattga tgttgataaa cacgatagtg caattataca
     3001 agcggcatta gacgaggcga atagtttgaa taaacttagc tttaaatata tggatcgtat
     3061 tttacttaat tggaagaaaa acaatgtcaa aacaattgac gattctagaa aaataagaga
     3121 aaaatttaat aaaccgaaaa tgacgcacac agttaaaacg gtacctaaat ttgattggtt
     3181 aaacggggag aaccttgatg gtaagtaaga aaaaagcatt agaaatgatt gatgttatag
     3241 caaatatgtt tccggatgca gaatgtgaat taaaacatga taatccgttc gaattaacta
     3301 ttgctgtatt attgtcagcg caatgtacag acgttctagt aaatagagta acaactgagc
     3361 tgtttaaaaa atacaaaacg cctgaagatt atttggcagt cagtgatgaa gaacttatga
     3421 atgatattcg ttctatcggt ctgtatcgta acaaagctaa gaatattaag aaactttgtc
     3481 aatctttgat tgatcaattt aatggagaaa taccacaaac acataaggaa ttagaaagtt
     3541 tagcaggtgt aggacgtaaa actgctaatg tagtcatgag tgtagcattt gatgaacctt
     3601 cattagctgt tgatacgcat gtagaacgtg tttctaaacg cttaggtatt aatcgttgga
     3661 aagataatgt gagacaggta gaagaccgtt tatgttctgt catacctaga gatagatgga
     3721 atagtagcca tcatcaactc attttctttg gaagatacca ctgtttagcc cgaaaaccta
     3781 aatgcgatat ttgtccacta ttagaagatt gtagagaagg acaaaaacgt tataaagcta
     3841 gtttgaaaga agcgtgataa catgacaaat attacaaaag aggtatttga taatttagaa
     3901 caagagattg atgtatttgc caaaaataag acattaggtt ctagtgaagc aaagccctac
     3961 ttggatgaat accatagtaa aattattgat tattttaagc aggttaatga cataactggc
     4021 aatattgatt ttgataaatt aaatcaatat cctgttgtgc caatgaattt taaagaaaga
     4081 tatgattata tgattgaacg taaatatcat tttatgggct atcgacaaat gaagaccttt
     4141 aaaactgaat tgattaaaat gaatgctagt tatcaaacaa ggttaaagaa taagcaggta
     4201 tagacaagat tgatcatttc ctaatgttga aaatatataa aactaattca tgcacacata
     4261 tttgtattta tgttaggtgt cgtgtcatta caacgtctta atataaaaac tgtccatcta
     4321 catcataatg gatatcttga gatatgcttt aagataccta tttcgaatcg taggtggatt
     4381 tcttatatct atgattgtga aaatatgttg caaacatctt gatatttcac tgattaacgt
     4441 acacagggaa ttgataaaca tttcaattta aaacagcgtc taagatgact atgagaaaaa
     4501 taatcataat tttaaaaatg ggaacaacca cttttgcaat gtcaataatt ttaaatagct
     4561 aaagatgtga atatgaaaaa ctatttagat gtttcaaaat gtatgtgaat gactgatttt
     4621 aaggtttcta ctaatatagc aatagcgtat gaatacttat aaaaaaagca ggaagtttta
     4681 ccttcccacc ataaaagatg aagaacctaa aaaatcctca gctacgccat aaatatggta
     4741 taactgagga ttttatgttg tgtggattag ttgaatatac ctgttaatcc accgctgttt
     4801 ctagattgtt gagatctagt attgttattt gattgtgcag taccactgct gtttgctgat
     4861 gtgtcactac caccgtgtgt actacgattt gtagtgttgt tatcttgact accagaaaca
     4921 ttgattgatg ggatactacc acttactgag ctaggacgtt taaagtcttc gccatctcta
     4981 gatgaaattt ttgacatcac attttcatat aagaactgtg gatattcttg ttggctatgt
     5041 cccacaaatg agttttcacc atattgttta actttactga agcccatcca cactgacata
     5101 gtgtattgag gtgtaaagcc gttaatccac acgtctttcg ctgcattatc aggtaaatta
     5161 tattgtgaat aagtttcagc accgtaagta ccagtacctg tcttagcacc catatttact
     5221 ccagatacac catggccata tgcagaacca tatggtttaa atgtaccttt tagcatctca
     5281 gctaacatgt atgcagtgta atcactcatc gctttatggc tagtatgatc gtattcgatt
     5341 gtttcaccat cacgagtaac tactttttga attgaatgcg cgttgttata agtaccaccg
     5401 ttagcgattg cagcaaatgc tgatgctaat tgtgttggtg agaattctga agcagaacca
     5461 ccaagtactt cagatggacc aatatcgcct tcgtagttta agccaagttt ggcagcgaat
     5521 ttcttaggtg catcattacc agcattttgc ttaactgatt gccaagcttt taaagctggt
     5581 atattgaaac tttgtcgtaa agcatcataa atagatacag taccgtgact cttcgtatca
     5641 tagtttctaa atgtagaacc atcaacttga tatgaagatt catcttgaat cgcatggttt
     5701 gttgcccatt tcatattttc aatggcaggt ccatacgcta agaaaggttt taaagatgaa
     5761 ccagtagggt gaggatctgt tgcttggttt ctgttaacga cgtctttgaa atcacgtcca
     5821 ccagatatag caactaaacc accagtttta ctatcaagaa tcgttgcacc aacttgttgg
     5881 tctttattct tgtagaagct accattatca acatcatttt gtaatgtttt ttgaacatct
     5941 ttatccatgt ttgtataaat tttaatacca ctttgtaata cattacctaa attttcatct
     6001 ttgaatgctt tattattcat taattcagat tttacaaagt taacgtatga attatactca
     6061 gaatcttgat ttgtatcaat gttttgacgt tcttcagcag tacgatttac taagttcgct
     6121 tttaaatcga ttttcttagc atcttcccac tgtttatctg taatgcgttt atgataatgc
     6181 attaagtata aaacagtgtt tttacgatct tcagcagctt ttggatgatc ataaatatta
     6241 tagttgtttg gaacctgagg taaaccggct aaataagctt cttccgctaa gtttaaatct
     6301 tttaaatctt tattaaagta atacttagca gcagctttaa tacctgttac gccatcagaa
     6361 tagtaaattt tatttagata tacttggaag atatcatctt tactatactc ttgttctaaa
     6421 cgatatgata agtatgcttc ttgagcttta cgtccaatag atttatgttg tgataaaaat
     6481 gcatctttaa caacttgttg tgttaatgtt gaggcacctt cagaaccaaa tccaccagtc
     6541 aagttcttac caattgcacc gaataaacgt ttataatcaa gtgcgccatg ttcgtagaaa
     6601 cgattgtctt cagttgcaag tactgcgtct ttcattgatt tcggcacgtc ttttaaattt
     6661 acatgctcat gtctttggcc attatctaat gttttaacaa gttctccgtt cttgtcatat
     6721 atctttgcag gaatcggatc ttgtaattta gcttcggtaa aagcaggtgc tttccaagca
     6781 taataagcaa acaataagat acctagtaaa agaacaacga aaaatgcaat aatcatgaag
     6841 ccaataatct taataatcgt tctcttcaca tttctattct tttttgagtt ggatttccca
     6901 ccattattac catttttctt aggctgagaa gatcctttgt tttccgtcat acgcggtcct
     6961 cactttcatc taatatcaac ttatcaactg cttttagata gtctaatctt ggttgatact
     7021 gataaggaat atggtaacca ttttttcgta tttcatcaac tgttatagac tttttaatat
     7081 tatctttata tctcttccaa aatacttcga attttgaata gggtaaaaga taaacttcat
     7141 ctagcgtttt aaaacgaatc attaaaaaca caataccttt ttgttgatat gcatttttca
     7201 tatgttcgac ttgatggtca tgaatattat ttaaaggaaa ggacgtcttg tttttagttt
     7261 cctttgcttc aaaatcaata taataacctt gataaacgcc gttgtaatca gttgttgaag
     7321 gtgtacgaaa ataagcttcg ttaatcacag ctttactccg cttaggatag ttgacattaa
     7381 ctatttgtac tggcgtaggc tttttgtgaa taactgcaat gtcgctttta agataaaacg
     7441 tatttgaatg ttcgatatct ttttcaagtg acataccacg tccaccatac tcaatattac
     7501 taaaggcagc ggtctttttc cctccgtcta tagcactatt tttacgatat ggtttaccat
     7561 ttggataatt cataaatttc accacacaag tttggtagta aacgttgttt attatacaca
     7621 acattatagt gaaacaccaa acttaaatta gtataaatgc gaaagacaca atacaccaaa
     7681 gcttatttta acgtgatttc agcattaaca caataatatt ataatacatt taccgttaat
     7741 gttggaattt tacacacaat tttaattaat tgaaaagttt tgcttataaa aatgaatact
     7801 cattacaata tttttgaatt ttgttataaa agtcaaatca tgatcagtaa ttattatttt
     7861 tgaggcaagt acaag
//
LOCUS       DAMZKC010000115         7778 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.116, whole genome shotgun sequence.
ACCESSION   DAMZKC010000115 DAMZKC010000000
VERSION     DAMZKC010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7778
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.116"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(115..441)
                     /locus_tag="POB55_001922"
     CDS             complement(115..441)
                     /locus_tag="POB55_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HDI6500882.1"
                     /translation="MILDNVNPNDLFPTEKKGPSVLGIIEYKVQGENEFEGAFIATNE
                     RLIMNVDMNGQFYYRSISYNEIEKIDYDGQTIMFKFNIGNVPMHDIKSDNVEMFVEYV
                     KQHMIV"
     gene            complement(505..1248)
                     /locus_tag="POB55_001923"
     CDS             complement(505..1248)
                     /locus_tag="POB55_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SarA family transcriptional regulator"
                     /protein_id="HDI6500883.1"
                     /translation="MSKFKVKRLTAHILMLKVINDMLKYSFHLTLTQVKLLNLLVKYD
                     NSDSNSASIDSLLKMKEIQSKMALLQMLSYLNNHQWLLKGRDQRDQRKLTISINKMHI
                     DKIEYMNNELSDYIERYFGQYTFDFSCDYVSYLLSSQELFRNLKCYLNMCQLTLEELY
                     VLGILNLHKGQLTVKELQGEFHHPIFAVSPILKCLILKGLVKKERCELDERRVIVTIK
                     REKFSKVTMLIQACYNYLEKGIQVKLNNK"
     gene            complement(1275..3230)
                     /locus_tag="POB55_001924"
     CDS             complement(1275..3230)
                     /locus_tag="POB55_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI6500884.1"
                     /translation="MTDNYSLHIYKSMKMSSVKEANLKIIYWLQGTGVVSINLQQYDV
                     KRNDVTVIMLNDLYQIDGHDDSVCCVVEVSAKTFLKFMNANYMMRGKILTNEVASTFR
                     ILIKYLIYSKIKQQSYNANDKIINYMCVELMSLNTYDGSRHLVAEEVHDYLTNNHHKK
                     INRKDVINQVSITNKALSDMVKATPYSNFIQYLNHIRLEHCLIDILTSKKPIEEIASS
                     HGFNHYSRFIHLFKETYGNTPKLIRKRYSPTSHSINHSQLVEIDKNIIELFDDTNQSS
                     SSMREIDIPFEPTKRSQMYQPKNIYIKGSNFYWMDYYTIKQIIQRLGVNPENLHIIVT
                     IDTSNELSIEQITLLLQKIVNYNVNVVFRIKHEYKELLTSTERGKIEKLVATIFNMTF
                     ESNRCKIAFLINDLNTTAIQKLKRLIDNYIGVFELIYHLEPKELNTRYDKEIDHLIDY
                     FILPLRQIEQVSIACSKIICDVDLINENNDLKEKITMSDFNKVTELLTNYTKFRGLIM
                     HMSIISEASKEINLTRLSIFIFIINILNQLRGIVIYQNDTMIVTKFKHEVQCVICLPA
                     ALLNENSDAIRLSCKGLQQFSHAKTKQIVLSIDKDNHTSENGVLVNDIMQQHSYWKAN
                     FIERNIYDHSLMVSSNSIVHLKYHLEI"
     gene            3840..4649
                     /locus_tag="POB55_001925"
     CDS             3840..4649
                     /locus_tag="POB55_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDI6500885.1"
                     /translation="MKKIATATIATAGFATIAIASGNQAHASEQDNYGYNPNDPTSYS
                     YTYTIDAQGNYHYTWKGNWHPSQLNQDNGYYSYYYYNGYNNYNYNNYNNGYSYNNYSR
                     YNNYSNNNQSYNYNNYNSYNTNSYRTGGLGASYSTSSNNVQVTTTMAPSSNGRSISSG
                     YTSGRNLYTSGQCTYYVFDRVGGKIGSTWGNASNWANAAARAGYTVNNTPKAGAIMQT
                     TQGAYGHVAYVESVNSNGSVRVSEMNYGYGPGVVTSRTISASQAAGYNFIH"
     gene            complement(4666..4908)
                     /locus_tag="POB55_001926"
     CDS             complement(4666..4908)
                     /locus_tag="POB55_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500886.1"
                     /translation="MTKLIENLKASIFVSTLTKNLSMYSRFDMPKFGCVNGCMLFILY
                     YRVNRIGENRNFEIFTNSIFFIEIKKANKALETLLA"
     gene            complement(5082..6482)
                     /gene="nhaC"
                     /locus_tag="POB55_001927"
     CDS             complement(5082..6482)
                     /gene="nhaC"
                     /locus_tag="POB55_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDI6500887.1"
                     /translation="MMTRKPTFLESISTMIVMVIVVVTGFVFFDIPIQVLLIIASAYA
                     TWIAKRVGLTWQDLEKGIAERLNTAMPAILIILAVGIIVGSWMFSGTVPALIYYGLDL
                     LNPSYFLISAFFISAVTSVATGTAWGSASTAGIALISIGNQLGIPPGMAAGAIIAGAV
                     FGDKMSPLSDTTNLAALVTKVNIFKHIHSMMWTTIPASIIGLLVWFIAGFQFKGHSND
                     KQIQTLLSELAQIYQINIWVWVPLIVIIVCLLFKMATVPAMLISSFSAIIVGTFNHHF
                     KMTDGFKATFSGFNDSMIHQSHISSSVKSLLEQGGMMSMTQILVTIFCGYAFAGIVEK
                     AGCLEVLLTTISKGIHSVGSLICITVICCIALVFAAGVASIVIIMVGVLMKDLFEKYQ
                     VSRSVLSRTLEDSSTMVLPLIPWGTSGIYYTNQLHVSVEEFFIWTVPCYLCAIIAIIY
                     GFTGIGIKKSSNSRLT"
     gene            complement(6577..7659)
                     /locus_tag="POB55_001928"
     CDS             complement(6577..7659)
                     /locus_tag="POB55_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HDI6500888.1"
                     /translation="MKIAIVGSGNGAVTAAVDMVSKGHDVKLYCRNQSISKFQNAIEK
                     GGFDFNNEGDERFVKFTDISDDMEYVLKDAEIVQVIIPSSYIEYYADVMAEHVTDNQL
                     IFFNMAAAMGSIRFMNVLEDRHIETKPQLAEANTLTYGTRVDFENAAVDLSLNVRRIF
                     FSTYDRSCLNDCYDKVSSIYDHLVKEESLIKTNLENGNPEVHPGPTLLNVGRIDYAGE
                     FALYKEGITKHTVRLLHAIELERLNLGRRLGFELSTAKESRIERGYLERDKEDEPLNR
                     LFNTSPVFSQIPGPNHVESRYLTEDIAYGLVLWSSLGRVIDVPTPNIDAVIVIASTIL
                     ERDFFEEGLTVEEIGLDKLDLEKYLK"
BASE COUNT         2674 a         1419 c          986 g         2699 t
ORIGIN      
        1 caaaatttat aaaatataaa aaaagaagag ctttaaatca taacgcatgc catttatctt
       61 taaatgcaat gagttatcat tcaaactctt cttatcaacc tacatacatt gacattatac
      121 tatcatatgt tgttttacat attcaacaaa catttcaacg ttatcacttt taatatcatg
      181 cattgggaca ttaccaatat taaatttgaa cattatagtt tgaccatcat aatctatctt
      241 ctctatttca ttataactaa tacttctata ataaaattgg ccattcatat ctacattcat
      301 aattaatctt tcatttgttg ctataaatgc cccttcaaat tcattctcac cttgaacttt
      361 atactcaatt attcctagca ctgacggacc ctttttttca gttggaaata aatcatttgg
      421 attcacatta tctaaaatca taatatccct cccacttaaa actataaact attcttcata
      481 ggtatgtgaa atacaagtga ttaactattt attattaagc ttaacttgta ttcccttttc
      541 aagataatta taacatgctt gaataagcat agtgacttta gaaaactttt ctcgcttgat
      601 tgttacaata acacgccttt catctaattc acatctctct tttttaacta atcccttcaa
      661 aattaaacac tttaaaatcg ggctaaccgc aaatatcggg tgatggaatt caccctgtaa
      721 ttctttaacc gtcaattgac ctttatgcag atttaatatt cctaaaacgt ataactcttc
      781 taatgttaat tgacacatat ttaaataaca ctttagattt cgaaataatt cttgtgacga
      841 caataaatac gatacatagt cacaactaaa atcaaatgta tattgaccaa aataacgttc
      901 aatatagtca ctaagctcat tattcatata ttctatttta tctatatgca ttttattaat
      961 cgaaatagtc aatttacgtt gatccctttg atctcttcct ttcaatagcc attgatgatt
     1021 gtttaaataa cttaacattt gaagaagggc catcttagat tggatttctt tcatttttaa
     1081 taaactatct attgaagcgc tattggaatc agaattatca tatttaacta atagatttaa
     1141 tagtttcact tgagtcaaag tgagatgaaa tgaatatttc aacatatcat taatgacttt
     1201 caacattagt atgtgtgcag ttaaacgctt aactttaaat ttagacatct ttaaaaacct
     1261 ctcttaaacc atgcctatat ctcaagatga tatttcaaat gaacaatact attgcttgag
     1321 accattaatg aatgatcata aatatttctt tctataaaat tagctttcca ataactgtgt
     1381 tgttgcataa tatcattcac aagtacacca ttttcggaag tatgattatc tttatctata
     1441 cttaaaacaa tttgtttagt tttagcatgg ctaaattgtt gaagaccctt acacgataaa
     1501 cgtatagcgt ctgaattctc atttaacaat gcggctggca aacaaatgac acattgtact
     1561 tcatgtttga acttcgttac aatcatcgtg tcattttgat aaataacaat ccctcgtaat
     1621 tgattaagta tatttatgat aaaaatgaat attgataatc tagttaaatt tatttctttg
     1681 ctcgcttcac ttataattga catgtgcata ataagtcctc gaaattttgt ataatttgtc
     1741 aaaagttcag taaccttatt aaaatcagac attgtgattt tttcttttaa gtcattattc
     1801 tcattaatca aatctacatc acatataatt ttagaacatg caatagacac ctgttctatt
     1861 tgtcttagcg gtaagataaa atagtctatt agatgatcaa tttctttatc atagcgcgta
     1921 ttcaactctt ttggttccag atgatatatt aattcaaata ctcctatata attatctatt
     1981 aaacgtttca gtttttgaat tgcagtagta tttaagtcgt taattaaaaa tgcaatctta
     2041 catctattac tctcaaaagt catattaaaa attgtagcta ctagcttttc tatcttgcct
     2101 ctttcagtac tcgttaacaa ttccttatat tcatgtttaa tacgaaatac tacatttaca
     2161 ttgtaattca ctatcttttg aagtaataaa gtgatttgtt caatcgatag ctcattgctt
     2221 gtgtcgattg taacaattat atgcaagttt tcaggattaa cacctaatct ttgaatgatt
     2281 tgtttaatag tataataatc catccaataa aaattacttc ctttaatata aatgttttta
     2341 ggttgataca tttgactcct ttttgtaggc tcaaaaggta tatcaatctc gcgcatactt
     2401 gaagaacttt gattagtatc atcaaataat tcaattatat ttttatcaat ttcaactaat
     2461 tgggaatggt taattgaatg agatgttgga ctatatcttt ttctaattaa tttaggagtg
     2521 ttaccatacg tttctttaaa gaggtgtata aaacgagaat aatgattaaa accatgacta
     2581 cttgcgattt cttcaatagg cttcttagaa gttaaaatat caattaagca atgctccagt
     2641 ctaatatgat ttaaatattg aataaaatta ctataaggtg tcgctttaac catgtcactt
     2701 aaagctttgt ttgtaatact aacttgatta atgacatctt tcctatttat ctttttatgg
     2761 tggttgtttg ttaaataatc gtgcacttct tcggctacta aatgacgact accatcgtat
     2821 gtatttaatg acattaattc aacacacatg taattaataa tcttatcatt agcattataa
     2881 gactgttgtt taattttgct gtagattaaa tacttaatca agattctaaa agtgctagca
     2941 acctcatttg ttaatatctt gccacgcatc atatagtttg cattcataaa cttcaaaaat
     3001 gtctttgcac ttacttcaac aacacaacat acactatcat catgaccatc aatttgatac
     3061 aaatcattca acataataac ggtgacatca tttcttttta catcatattg ttgtagattg
     3121 attgaaacta cacctgtacc ttgtagccaa tatataattt ttaagttcgc ttccttcaca
     3181 ctactcattt tcatactttt ataaatatgt agtgaataat tatctgtcat cttacatcct
     3241 cacatataca aatatattga tttactcctg tttgtctttc tcaaatcaat tgtcaacatc
     3301 taatattcac taccaattat atcgccacat gttttttagc agtaacaaaa cataaatcga
     3361 ttatatcaaa taatactttt attaaaacat ttttttatgc taaattagga tattttatcc
     3421 attaaatata cattttcatc caaataccaa agctttcata atcatagaaa cttgatcgtt
     3481 cgtgtcgaat ttttatgcat tttctgatta aaagtcctat attatgcaca aaatatacat
     3541 ttagaatttt cagtaatatt acaaatttgt aacagactta ttttcgaaac attcagtttt
     3601 cacaaagaat gccgtagtta taagttttgc aaaatgcaaa tttctatact tttaaaattc
     3661 tattgtgtca agatattagc aaaaaattac aaaacgttaa caaagcacac aaggacgcta
     3721 atttctgtta tagtctttct tgtcgtttaa ttacgagaga taacttataa cattaatttt
     3781 gtcacacgga cattacgaaa atattttaga tttaaaattt ttaggaggat atttttaaaa
     3841 tgaagaaaat cgctacagct actatcgcaa ctgcaggatt cgctacaatc gcaattgcat
     3901 caggaaatca agctcatgct tctgagcaag ataactacgg ttataatcca aacgacccaa
     3961 catcatatag ctatacttac actattgatg cacaaggtaa ctaccattac acatggaaag
     4021 gtaactggca tccaagtcaa ttaaaccaag ataatggcta ctacagctat tactactaca
     4081 atggctacaa taactataac tacaacaatt acaacaatgg ttatagctac aataattaca
     4141 gccgttacaa caactactca aataataatc aatcatataa ctacaataac tataatagtt
     4201 acaacacaaa cagctaccgt actggtggtt taggtgcaag ctacagcact tcaagcaaca
     4261 atgttcaagt aactacaact atggctccat catcaaatgg ccgttcaatc tcaagtggtt
     4321 atacttcagg acgtaactta tacacttctg gtcaatgtac atactacgta tttgatcgtg
     4381 taggtggtaa aatcggttca acttggggca atgcaagtaa ctgggctaac gcagctgcaa
     4441 gagctggtta cacagtgaac aatacaccaa aagctggtgc aattatgcaa acaactcaag
     4501 gtgcatacgg tcacgttgca tacgttgaaa gtgttaacag caatggttca gtaagagttt
     4561 cagaaatgaa ctatggttat ggcccaggtg ttgtaacttc acgtacaatc tcagctagcc
     4621 aagctgctgg ttataacttc attcactaat tagtgatggc tatgtttacg ccaacaaggt
     4681 ttcaagagcc ttgttggctt ttttaatttc tatgaaaaaa atcgaattgg taaaaatctc
     4741 aaaatttcga ttttcaccaa tccgattcac tctgtaataa agaataaaca acatacagcc
     4801 attgacacac ccaaatttag gcatgtcaaa tcggctatac attgataaat ttttagttaa
     4861 agtacttaca aatatacttg ccttcaaatt ctcaattaat ttagtcatta caaatcatga
     4921 cataaaactt acattacata tatcaacatg ttccgctaca aaacatattc agtatatgta
     4981 tagaggatat ttattctaag ttaaaacctt ttcaaactat aaccgctaca attataaatg
     5041 attaaagtgt ttcaacatat tatatatatt ccacgctcac attaagttaa acgtgaattc
     5101 gatgactttt taatacctat ccctgtaaaa ccatagataa ttgctataat tgcgcataaa
     5161 taacatggta ctgtccatat gaaaaattct tcaacagaga catgaagttg attcgtatag
     5221 taaatacctg atgttcccca tggtattaaa ggtaagacca tcgtacttga gtcttcaagt
     5281 gttcttgata aaacggaacg agagacttgg tatttttcga acaaatcttt cattaacaca
     5341 ccgaccataa taattacaat cgaagcaaca cctgcagcga atacaagcgc aatacaacaa
     5401 ataacagtaa tacatattaa acttcctaca gaatggatgc ctttagaaat agtagttaat
     5461 aagacttcta aacatcctgc tttttctaca atacctgcaa atgcatatcc gcaaaatatc
     5521 gttactaata tttgggtcat actcatcata ccaccctgtt ctaacaagct tttcacactg
     5581 gatgaaatat gagactgatg tatcattgag tcgttaaaac cactaaatgt tgctttgaaa
     5641 ccatctgtca ttttgaaatg atgattaaaa gtccccacta taatggcaga aaagcttgat
     5701 attagcatag ctggcactgt agccatttta aatagcaaac aaacaatgat cacaattaag
     5761 ggaacccaga cccatatgtt aatttgataa atctgtgcaa gctctgataa caaagtttga
     5821 atctgtttat catttgaatg ccctttaaat tgaaatccag caataaacca tactaataaa
     5881 cctatgattg atgcaggtat cgtcgtccac atcatcgaat gtatatgttt aaatatatta
     5941 actttagtaa caagcgccgc taaattagtt gtatctgata atggtgacat tttatcgcca
     6001 aacacagccc ctgctataat agcacccgct gccatccctg gaggaatccc caattgatta
     6061 ccaatagaaa taagtgcaat ccctgcagtt gatgcagagc cccatgctgt accagttgct
     6121 acagatgtaa cagcacttat aaaaaaggct gatattaaaa aatagcttgg attcaataaa
     6181 tctaagccat aataaatcaa ggctggcact gtgccagaaa acatccaact gcctactata
     6241 attcctaccg ctagtataat taaaattgca ggcattgcag tatttaaacg ttctgcaatg
     6301 cctttttcta aatcttgcca tgttaagcct acacgttttg caatccatgt ggcatatgct
     6361 gaggcaataa ttaataatac ttgaattgga atatcaaaaa acacaaagcc tgttacaaca
     6421 acaataacca ttacaatcat tgtcgaaatt gattctaaaa aggttggttt tcttgtcatc
     6481 atctatcgtc cccttcgctt attgatgaga aacctctttt cttcaattta ttgcttttgc
     6541 ataatgctat atccttttct atcttcaagc catcatttat tttaaatatt tttctaaatc
     6601 aagcttatct aaaccaattt cttcaactgt taagccttcc tcaaagaagt ctctctctaa
     6661 aatggttgat gcaattacaa ttactgcatc tatatttggt gtcggtacat caataacacg
     6721 acctaagctt gaccatagta ctaaaccata tgcaatatct tcagttaaat atctgctttc
     6781 tacatgattt ggtcctggaa tttgtgaaaa tactgggctt gtattaaaca aacgatttaa
     6841 tggttcatct tctttatcac gttctaaata accacgttca atacgtgatt ctttagctgt
     6901 tgataattca aaacctaatc tacggcctaa attcaaacgt tctaattcga ttgcatgaag
     6961 taatctaact gtatgtttag taattccttc tttatataaa gcgaactcgc cagcataatc
     7021 aatgcgaccg acattcaata atgttggtcc tggatgcact tcaggattac cattttcaag
     7081 atttgtttta attaagcttt cctcttttac taaatgatca taaatacttg aaactttgtc
     7141 ataacaatca tttagacagc ttctatcata tgttgaaaag aagatacgac gtacatttag
     7201 agataaatca actgctgcat tttcaaaatc gacacgcgta ccatacgtca acgtattagc
     7261 ttccgctagt tgtggttttg tttcaatatg tctatcttct aaaacattca taaaacgaat
     7321 tgaccccatt gctgcagcca tgttgaagaa tatcaactga ttatcagtta catgctctgc
     7381 cattacatca gcataatact ctatgtatga agatggaata atcacttgaa caatttcagc
     7441 atcttttaaa acatattcca tatcatcact aatatcagtg aattttacga aacgttcatc
     7501 accttcatta ttaaaatcaa atccgccctt ttcgattgcg ttttgaaact tacttataga
     7561 ttgattacga caatataatt taacatcgtg gcctttgctc accatatcta ctgctgccgt
     7621 aactgcgcca tttcctgatc ctacaattgc tattttcata aacaaccctc tttctatctt
     7681 tggttaaaat taaaaacaaa ttatacgtaa gacaatataa aaatgatgcg aattcattac
     7741 gcatcattat aaatgttaag tatatataac cttaaatt
//
LOCUS       DAMZKC010000116         7720 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.117, whole genome shotgun sequence.
ACCESSION   DAMZKC010000116 DAMZKC010000000
VERSION     DAMZKC010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7720
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.117"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(229..921)
                     /locus_tag="POB55_001929"
     CDS             complement(229..921)
                     /locus_tag="POB55_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HDI6500889.1"
                     /translation="MNYSPKQGVRSHGLPHDPFKSSTVPRPIGWISTVSKDGKDNLAP
                     YSQYQNLTWDPPMVMFAANQSVLGDHERKDTVKNAEETGWFVWNMATYDLREAVNLSS
                     KALPPEEDEFNFAGVTKEKCIEAPGYRVKESPVHFECEYVQTIRIPTGDPVSTVDIVI
                     GRVAQVHIDDKVILDNGKLDIKSIKPIARLGYYDYTVVNEIFEMKAPAASKEELAGLE
                     GRNFDNQSDEKI"
     gene            1234..2178
                     /locus_tag="POB55_001930"
     CDS             1234..2178
                     /locus_tag="POB55_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500890.1"
                     /translation="MRKIMRILLCASIIVSGCSFIPKNQTKSIPKTVSVKDYDGKYIG
                     EHKKRNEVFLKKHKDEAIKKYKDYVKDTFGYDCKINLVDAYTNKSGFSEKSKVDGISV
                     VGTVNYDVPFQLQLLFVESGNGITISTFTPGHINETSAAVSAMMYKRYEHEIEQARLK
                     LKSEIEKDSYYAMSEKLQVKQEFNGVTRQYLNFRTDSIDDLDKFKKEFKPVMHLKGDA
                     FNQQLQSLINKYPQIQKNMKSEFIAYYDKEKNRETVKNYAWNLQKSINDIMQSYPSTK
                     FVQFYKDDVSPSMVDGNGRLKSDTNVISIEGGKYDENK"
     gene            2165..2467
                     /locus_tag="POB55_001931"
     CDS             2165..2467
                     /locus_tag="POB55_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500891.1"
                     /translation="MRTNNHITDLDAIELSSGWNYNSKLETGKNALLSDSLLRDIKVL
                     SSNAKGDADILETELRAVINAMSQNEGKSVDALKNSTAPLKNNLTKLKEQLDKTTH"
     gene            2621..2980
                     /locus_tag="POB55_001932"
     CDS             2621..2980
                     /locus_tag="POB55_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500892.1"
                     /translation="MILRYATNLKLAKELYQASKPKLQEDKGMIELFEHTFGLQKELV
                     KYVGIAEATTAALYSASFINKNISRLASLSTIGILSVAAYKHFEAGQGKKGAQHALDL
                     LGLATLSLLDTFSCNKK"
     gene            complement(3065..3676)
                     /locus_tag="POB55_001933"
     CDS             complement(3065..3676)
                     /locus_tag="POB55_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HDI6500893.1"
                     /translation="MYTFDAQSLTARENYKLLIGSIIPRPIAFVTTLNQDASVNAAPF
                     SFFNIVNNHPPMIAIAVQRAGGKRKDTALNIERTGDFVVHITDEDNVQDINETAAPLA
                     YGDSELSRTELSLLTSTTIKTPGIKDAKMRFECKLSQMILLGDVLDGADLIIGEIVTY
                     HIDDSIYEGDFKINPHALQAVSRLAGNDYAKLGGIFTIKRPEK"
     gene            complement(3755..4231)
                     /locus_tag="POB55_001934"
     CDS             complement(3755..4231)
                     /locus_tag="POB55_001934"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HDI6500894.1"
                     /translation="MAVVYVAGGCLWGVEAFFATIPGIIHTEAGRANGRSSKLDGPYD
                     GYAECVKLHFDDRMLTITDIMNYLFEIIDPYSVNQQGNDIGQKYRTGLYSCVDDHLIE
                     ARQFIERRKDRDQIAVEVLPLSNYIKSAEEHQQHLEKYPEDMHMCHISKDLLNKYK"
     gene            complement(4234..4734)
                     /locus_tag="POB55_001935"
     CDS             complement(4234..4734)
                     /locus_tag="POB55_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6500895.1"
                     /translation="MVLRKVIMQDLDQIIALENIGFSPEEAATREALKLRIEQIQETF
                     IVAEKNDEVIGYINGPVIKERYISDDLFKNVSTNNSEGGYISVLGLVVAPNYQGQGIA
                     GRLLNYFETLAKNHHRHGVTLTCRESLISFYEKYGYRNEGVSESCHGGIKWYNLVKNI
                     KKDDEV"
     gene            complement(4969..5736)
                     /locus_tag="POB55_001936"
     CDS             complement(4969..5736)
                     /locus_tag="POB55_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HDI6500896.1"
                     /translation="MIDIHNHILVDIDDGPKTIEKSIALLKQAKDEGVTSIVATPHHL
                     HPRYDNTFQQVLVKLAELRTHPEVQALDIKLFPGQEIRITDSILKGLDNGSIQGINRS
                     KYLLIEFPTGEVPHYTKQLFFEIQSRGYIPIIAHPERNRSIAKNPEILYELVANGALS
                     QLTSSSLVGGFGKNIQKLSLQFIECNLAHFVASDAHSCDQRPFLMQELFHNHKLKKYS
                     NDIEALLRNASSVINDNFVYLDRPTKPGKVKSFLKWF"
     gene            complement(5733..6425)
                     /locus_tag="POB55_001937"
     CDS             complement(5733..6425)
                     /locus_tag="POB55_001937"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HDI6500897.1"
                     /translation="MTNTRRSTSSLIVHEQPKSPISEKFRGIRSNIMFANPDSAVQSI
                     VITSEAPGAGKSTIAANLAVAYAQAGYKTLIVDGDMRKPTQHYIFNLPNNEGLSSLLL
                     NWSTYQDSIISTEIQDLDVLTSGPIPPNPSELITSRAFANLYDTLLMNYNFVIIDTPP
                     VNTVTDAQLFSKFTGNVVYVVNSENNNKDEVKKGKELIEATGAKLLGVVLNRMPKDKS
                     ASYYAYYGTDES"
     gene            complement(6442..7104)
                     /locus_tag="POB55_001938"
     CDS             complement(6442..7104)
                     /locus_tag="POB55_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CapA"
                     /protein_id="HDI6500898.1"
                     /translation="MKEKFDLVKLLNILKKNIKLLLILPAICLVVSAALTFFVMPDKY
                     TASTQILVNMKKSSSDLAFQNVQSSLQSVNTYTEIIKSPRILDKVSREFDGQYSTAEL
                     NSFLKVTNQTNSQIITVSVTTGNKSESDKIVNKISKVFAHDMPKIMSVDNVTILSSAH
                     DNAVKVSPIVSVNLVISIIVGIVLAILIIFLKELLDKRIKTEEDVESQLGLPILGSIQ
                     KF"
BASE COUNT         2590 a         1372 c         1087 g         2671 t
ORIGIN      
        1 aaacgataaa tattatatgc aaaatacatc tttaatatta aagtaatacc aatatttttt
       61 caataaacct agtgtaatat atgtgtaatt ctaaaagatt cttctttaaa aatataaata
      121 ccacgacata ttgctttaac attttcattt ataaagcgaa aaaatgcatc gctactaagt
      181 tgaatgttta gtaaggatgc attgaattca ctaaaatgat taaattactt atatcttttc
      241 atctgattga ttatcgaaat ttcttccttc taaacctgct aactcttctt tagaagctgc
      301 aggtgctttc atttcaaata tctcattcac tactgtgtaa tcgtaatatc ctaatctggc
      361 aataggttta atcgacttaa tgtccaattt accattatca agaataacct tatcgtcaat
      421 atgaacttga gcaactcttc ctataacaat atctacggta gatactggat ctccagttgg
      481 aatacgaatc gtttgaacgt actcacattc aaaatgaact ggcgattctt ttacacgata
      541 tcctggagct tctatacatt tttcctttgt tacacctgca aaattaaatt catcctcttc
      601 tggtggcaat gctttcgatg ataaattaac tgcttctctt aaatcatacg ttgccatatt
      661 ccacacaaac caacctgtct cttcagcatt tttcactgta tctttacgtt cgtgatcacc
      721 aagaacggat tgatttgctg cgaacataac cataggcgga tcccaagtta agttttgata
      781 ctgactataa ggcgctaaat tatctttccc atctttcgat acagtagaga tccaccctat
      841 tggacgtggt actgtactac ttttaaatgg gtcgtgcggt aaaccatgac ttcttacacc
      901 ttgttttggc gaataattca tactatcttc accccttata agtaattaca tttaaggtta
      961 cgccctcttt acataagcgt ctaatataaa taaacaattt atttataagt agaaactata
     1021 tatgacgtgg ttgcttataa tttgcgttct tgatccgaaa atttcagata aggatttatg
     1081 caattaatat ttatgatatc ttttgtaaat ttaattaatt atagttactt caatcatgat
     1141 tagtttataa taatattgaa taacaatttt ttgatttttg tgtaatttat ctatcaactg
     1201 aaaaacagct aatacaaagg agctaatgaa gatatgagaa aaattatgcg aatcttattg
     1261 tgcgcatcaa tcatagtaag cggttgctct ttcataccaa agaatcaaac aaaatctatt
     1321 ccaaaaacgg ttagcgtcaa agattatgat ggtaaatata ttggtgaaca taaaaaaaga
     1381 aacgaagtat ttctgaaaaa acataaagat gaagcaatca aaaaatacaa agactacgtg
     1441 aaagacacct ttggttatga ttgtaaaatt aatttagttg atgcctacac taataaatct
     1501 ggatttagcg agaaaagtaa ggtagatggt atctcagtgg ttggtactgt caattatgac
     1561 gttccttttc aactccaatt gctttttgtt gaatctggaa atggtataac tatttcaaca
     1621 tttacacctg gtcatataaa tgaaacatca gcagccgttt cagccatgat gtacaaacgt
     1681 tatgaacatg aaattgaaca agctcgatta aagcttaaaa gtgaaattga aaaagacagc
     1741 tattatgcga tgagcgaaaa acttcaagta aaacaagaat ttaacggcgt tacaaggcaa
     1801 tacttgaatt tcagaacaga tagtatcgat gatctggata agttcaaaaa agaattcaaa
     1861 cctgttatgc acttaaaagg tgatgcattc aatcaacagt tacaatcttt gattaacaaa
     1921 tatccacaaa tacaaaaaaa tatgaaatca gagttcattg cttattatga taaagaaaaa
     1981 aatagagaaa cagtaaaaaa ctatgcttgg aaccttcaaa aatctataaa tgacattatg
     2041 caatcatatc ctagcacaaa atttgtacag ttttataaag atgatgtttc cccgtcaatg
     2101 gtagatggaa atggccgttt aaaatcggat actaatgtaa tttccatcga gggaggaaag
     2161 tatgatgaga acaaataatc atataaccga tttagatgct attgagttaa gtagcggttg
     2221 gaattacaat tcgaaattgg agactggcaa aaatgcacta ctatcagata gccttttaag
     2281 agatattaaa gtattaagtt ctaatgctaa aggcgacgct gacatactag aaacagaact
     2341 tcgtgctgta ataaacgcaa tgtctcaaaa cgaaggtaaa tctgttgatg cacttaaaaa
     2401 tagtaccgct cctctaaaaa acaatctaac caaattaaaa gagcaactcg acaaaactac
     2461 acattaattt catacatttt gagtgaacag aattatattt ccatagcaaa cattcctaaa
     2521 ctacacatac gttacataat tgattcattt ttatagaaac gggtaaaaat gataaagtaa
     2581 tcatctttca caaaaaatca aatattggga ggaattaaat atgattttgc gttacgcaac
     2641 gaatttaaaa cttgcgaaag agctatacca agccagtaaa cctaaattgc aagaagataa
     2701 gggtatgatt gaactgtttg aacatacttt tggcttgcaa aaagaacttg taaaatacgt
     2761 cggcattgca gaagcaacta ctgctgcttt atactcagct agctttatca ataaaaacat
     2821 ttctcgcctt gcatcactat caacgatagg cattttaagt gtagctgctt acaaacattt
     2881 cgaagctggc caaggtaaaa aaggtgcaca acatgcatta gatttattag gtcttgctac
     2941 tttaagttta ctagatacat tttcttgtaa taaaaaatag tcttcatttt agaagcacat
     3001 actttagcac ctgtgtactt ttgattcaat ccataaacgg tacaccgcta tagcatgtgt
     3061 tttattattt ttctggtctc tttatcgtaa atattccccc taatttagca taatcatttc
     3121 cggctaaacg agaaacagcc tgtaatgcgt gtggattgat tttaaaatca ccctcataaa
     3181 tactgtcatc aatatgataa gttacaattt cacctattat taaatcagcc ccatctaata
     3241 catctccaag caatatcatt tgcgatagtt tacattcgaa tctcattttc gcatctttaa
     3301 ttcctggcgt cttaatcgtt gtagatgtta aaagtgataa ttctgtacga ctcaactcac
     3361 tgtcaccata tgctaacggc gctgcagtct cattaatatc ttgaacatta tcttcgtctg
     3421 taatatgcac aacaaagtct ccagtccgtt ctatatttaa tgcagtatct tttctcttac
     3481 ctcctgcacg ttgaactgca atagcaatca ttggcggatg attattaaca atattaaaaa
     3541 agctaaatgg tgctgcattt actgatgcat cttgatttaa tgttgtaaca aaagctatag
     3601 gtcgtggaat aattgaacca attaataatt tatagttttc tctagcagtt aatgattgtg
     3661 catcaaacgt atacataata cctacctctt ttctaagtat atctaggtat ttctccgatt
     3721 ttggttaatt taaacatcta ttctcctctg aaaatcactt gtatttattt agcaaatctt
     3781 ttgaaatatg acacatatgc atatcttctg gatatttttc taaatgttgc tgatgttctt
     3841 cagcactttt aatgtagtta gacagcggta agacttccac tgcaatttga tctctgtctt
     3901 tacgtcgttc aatgaactga cgcgcttcaa ttaagtggtc atctacacaa ctatataaac
     3961 ccgttcgata cttttgtcca atatcatttc cttgttgatt cacactgtaa ggatcaatga
     4021 tttcaaataa ataattcata atgtctgtaa ttgttaacat acgatcatcg aaatgaagtt
     4081 tgacacattc agcataacca tcatacggac cgtctaattt agaacttctt ccatttgctc
     4141 ttcctgcttc tgtatgtata attccaggta ttgttgcaaa aaatgcttca acaccccata
     4201 aacatcctcc tgctacataa acaactgcca tatttacacc tcatcatcct tttttatatt
     4261 tttaacaagg ttataccatt taataccgcc atgacatgat tctgatacac cttcattacg
     4321 atacccatat ttttcataaa atgaaattaa tgattctcga catgttaacg ttacaccatg
     4381 tcgatgatga ttcttagcaa gagtttcaaa atagtttagt aagcgacctg caataccctg
     4441 accttgataa tttggtgcta caacaagacc taacacacta atatagccac cttcactatt
     4501 atttgtggag acatttttaa ataaatcatc gctaatgtaa cgctctttta tgactggacc
     4561 gttgatgtat ccaattactt catcattctt ttcagctaca ataaaagttt cttgaatttg
     4621 ttcaattctc aatttcaatg cctctcgtgt tgcagcttct tccggtgaaa aaccaatatt
     4681 ttcaagtgca ataatttgat ctaagtcctg catgattact tttcttaata ccataaaaac
     4741 acttccttag ccctatatat caaaagtaat accttcatct ttatattcat tattttcaaa
     4801 ctaccttcac tataaaaaac gagtcttttt attgaaagcc attcgcctta atcaattaga
     4861 caagttgtat tatacgaatt agtaaataat catgattata attcatttta agcatactca
     4921 aaaagactgg tacatgtata ccagtctaat tcgaaaaaat atatttagtt aaaaccattt
     4981 caaaaacgac ttcacttttc ctggcttagt cggtctgtct aaataaacaa aattatcatt
     5041 gattactgaa ctggcatttc gaagtaatgc ttcaatatca ttcgaatatt tcttcaattt
     5101 atgattgtga aataattctt gcatcaaaaa tggtctttgg tcacatgaat gtgcatctga
     5161 agctacaaaa tgagccaaat tacattctat aaattgtaat gataactttt gaatgttttt
     5221 accaaatcca ccaactaaag aactcgatgt taattgactc aatgccccat ttgcaaccaa
     5281 ttcatataat atttccggat ttttggcgat acttctattt ctttcaggat gtgcaatgat
     5341 tggtatgtaa cctctcgatt gtatttcaaa aaacaattgt tttgtataat gtggtacttc
     5401 gcccgttgga aattcaatta ataaatattt cgaacgatta ataccttgaa tactaccatt
     5461 atctaagcct ttcagaatcg aatctgtaat tctaatttct tgcccgggaa ataatttaat
     5521 atccaatgct tgaacttctg gatgcgttct taactccgcc aatttcacaa gcacttgttg
     5581 aaatgtatta tcatatctcg gatgcaaatg atgaggtgtc gctacaatac ttgttacacc
     5641 ttcatcctta gcttgcttta atagtgcaat actcttttca attgttttag gaccatcatc
     5701 tatatcaact aatatatggt tatgaatatc aatcatgatt catcagtccc ataatatgca
     5761 tagtaactag cacttttatc tttaggcatt ctatttaaga ctacacctaa taatttagca
     5821 cctgttgctt caataagttc ttttcctttt ttaacttcat ctttattatt attttccgaa
     5881 ttaactacgt agacaacatt gccggtaaac tttgaaaata attgcgcatc tgtaactgtg
     5941 ttcactggtg gcgtatcgat aattacaaag ttataattca tcaataatgt gtcatacaaa
     6001 tttgcaaatg cccttgatgt aattaactct gacggattcg gtgggattgg cccagacgtc
     6061 aagacgtcta aatcttgaat ttcagttgag ataatactgt cttgataagt tgaccaattt
     6121 agcaataaac ttgataggcc ttcattgttt ggcaaattaa aaatataatg ctgcgtaggt
     6181 ttacgcatat ccccgtctac gattagtgtt ttataacctg cttgcgcata tgcaactgct
     6241 aaatttgctg caattgtaga cttacctgcg cctggtgcct ctgaagtgat tacaatgctt
     6301 tgaactgcac tgtcaggatt tgcaaacata atatttgatc ttatgcctcg aaatttctcg
     6361 ctaataggtg actttggttg ttcatggaca attaaacttg atgtacttct tcgtgtattc
     6421 gtcatggtaa ttcctcgtaa attaaaattt ttgtattgaa cctaaaatag gtaatcctag
     6481 ttgcgattca acatcttctt ctgtcttaat acgcttatct aataattctt ttaagaaaat
     6541 aatcaatatt gctaaaacaa taccaacaat aatgctgata actaagttga cagatactat
     6601 tggagatact tttacagcat tatcatgtgc tgaggaaagt atcgtaacat tatcaacact
     6661 cataatttta ggcatgtcat gagcaaaaac tttagatatt ttattaacaa ttttgtcaga
     6721 ttcagattta ttcccagtgg taactgatac agtaataatt tgagagtttg tttgattggt
     6781 tacttttaaa aatgaattca actcagctgt tgaatactga ccatcaaatt ctctagatac
     6841 tttatctaga attctaggac ttttgataat ttccgtatat gtattaacag actgcaaact
     6901 actttgaaca ttttggaaag ctaaatcact tgaggacttt ttcatgttca ctaatatttg
     6961 agtagaagca gtatatttgt caggcataac aaaaaaggtt aatgccgcac ttactacaag
     7021 acatattgcc ggtaaaataa gcaataattt aatattcttc tttagaatat ttaatagttt
     7081 tactaaatca aacttttctt tcatggtttc ctccacataa tcaatcattg tattcattat
     7141 gtatgtttta taaatcggac aattatatct agtttaacga ccacaaaaca tacacaacta
     7201 cattttctct aattatttat ataaatattt tatcgtttaa aattatatca tgattctcta
     7261 ccattatgta taacttattt atatttttgc acaagatata atattgtcca actttaaata
     7321 tccaaaccta ttaataataa aactagatac catcgtactc tatcatggct ttcttataat
     7381 cgagtagaag catcatcatt acttgattat ttgctcttta caacaccgag cgtgcccgta
     7441 ctcggtaatt caataccttg cgtaacccgt cactgtgagt tgggttaatg ataataaagc
     7501 ccacaccttt taaaaagatg tgggtaattt atataatttt tatttacatt tttaacttat
     7561 aaaaaaaagc gcctatgtca tgatttacca tcacataggc gcttatcaat aaattattac
     7621 ttattacttt ccatttcatc taatttatgc ggatttcctg taattagatg acaacttatt
     7681 cttttcaggg gaacattaca cttttataat atgttcaaag
//
LOCUS       DAMZKC010000117         7659 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.118, whole genome shotgun sequence.
ACCESSION   DAMZKC010000117 DAMZKC010000000
VERSION     DAMZKC010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7659
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.118"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(136..963)
                     /locus_tag="POB55_001939"
     CDS             complement(136..963)
                     /locus_tag="POB55_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDI6500899.1"
                     /translation="MWKWEAENDAKGVVVIAHNILEHTGRYAYVITMLRRNGYHVIMG
                     DLPGQGQTSRANKGQIENFQTYHESLLDWLKIANEYKIPTYVLGVGLGGLILLNLLEK
                     VELPIEGMMLISPMLELQKNGKNRKDKLVSNIGKISKDTRFNVGVEPKDLTRNLEIVE
                     ETVNDGLMLKKATYHWYNTINETMKDTMAHIHDIQPMPTLLMYGTKDLIVDTRAIDEF
                     KEKYQTPELYFKAWQGFYHEVHNEPERDEVMRYILTFLNNSVNTMGFIVEDDEIVEI"
     gene            complement(966..1085)
                     /locus_tag="POB55_001940"
     CDS             complement(966..1085)
                     /locus_tag="POB55_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500900.1"
                     /translation="MMLISFIYNNMDNNNTINRNLHSEFAIIMLLKINEKGAV"
     gene            1197..2198
                     /locus_tag="POB55_001941"
     CDS             1197..2198
                     /locus_tag="POB55_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase"
                     /protein_id="HDI6500901.1"
                     /translation="MALLKNFFIGLSNNSFLNNAAKKVGPRLGANKVVAGNTIPELIN
                     TIEYLNDKNIAVTVDNLGEFVGTVEESNHAKEQILTIMDALHQHGVKAHMSVKLSQLG
                     AEFDLELAYQNLREILLKANTYNNMHINIDTEKYASLQQIVQVLDRLKGEFRNVGTVI
                     QAYLYDSHELVDKYQDLRLRLVKGAYKENESIAFQSKEDVDANYIKIIEQRLLNARNF
                     TSIATHDHRIINHVKQFMKENHIEKDRMEFQMLYGFRSELAEEIANEGYNFTIYVPYG
                     DDWFAYFMRRLAERPQNLSLAVKEFVKPAGLKRVGIIAALGATVMLGLSTIKKLCRK"
     gene            complement(2320..2784)
                     /locus_tag="POB55_001942"
     CDS             complement(2320..2784)
                     /locus_tag="POB55_001942"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HDI6500902.1"
                     /translation="MNFEGKLIGKDLKVAIVVSRFNDFITGRLLEGAKDTLIRHDVNE
                     DNIDVAFVPGAFEIPLVAKKLASSGNYDAIITLGCVIRGATSHYDYVCNEVAKGVSKV
                     NDQTNVPVIFGILTTESIEQAVERAGTKAGNKGAEAAVSAIEMANLLKSIKA"
     gene            complement(2797..3978)
                     /gene="ribB"
                     /locus_tag="POB55_001943"
     CDS             complement(2797..3978)
                     /gene="ribB"
                     /locus_tag="POB55_001943"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HDI6500903.1"
                     /translation="MQFDNIDSALMALKNGETIIVVDDENRENEGDLVAVTEWMNDNT
                     INFMAKEARGLICAPVSKDIAQRLDLVQMVDDNSDIFGTQFTVSIDHVDTTTGISAYE
                     RTLTAKKLIDPSSEAKDFNRPGHLFPLVAQDKGVLARNGHTEAAVDLAKLTGAKPAGV
                     ICEIMNDDGTMAKGQDLQKFKEKHQLKMITIDDLIEYRKKLEPEIEFKAKVKMPTDFG
                     TFDMYGFKATYTDEEIVVLTKGAIRQHENVRLHSACLTGDIFHSQRCDCGAQLESSMK
                     YINEHGGMIIYLPQEGRGIGLLNKLRAYELIEQGYDTVTANLALGFDEDLRDYHIAAQ
                     ILKYFNIEHINLLSNNPSKFEGLKQYGIDIAERIEVIVPETVHNHDYMETKKIKMGHL
                     I"
     gene            complement(3989..4621)
                     /locus_tag="POB55_001944"
     CDS             complement(3989..4621)
                     /locus_tag="POB55_001944"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HDI6500904.1"
                     /translation="MFTGIVEEIGVVKSVQIRQSVRTIEIEAHKITADMHIGDSISVN
                     GACLTVIDFDQTSFTVQVIKGTENKTYLADVKRQSEVNLERAMSGNGRFGGHFVLGHV
                     DELGTVSKINETANAKIITIQCSQHINNQLVKQGSITVDGVSLTVFDKHDNSFDIHLI
                     PETRRSTILSSKKLGDKVHLETDVLFKYVENILNKDKDQLSVDKLRAFGF"
     gene            complement(4628..5671)
                     /gene="ribD"
                     /locus_tag="POB55_001945"
     CDS             complement(4628..5671)
                     /gene="ribD"
                     /locus_tag="POB55_001945"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HDI6500905.1"
                     /translation="MSQFMDYAIQLANMVQGQTGVNPPVGAVVVKEGRIVGIGAHLRK
                     GDKHAEVQALDMAQQNAEGATIYITLEPCSHFGSTPPCVNKIIDCKITKVVYATKDNS
                     LDTHGDETLRAHGIEVECVDDERASQLYQDFFKAKAKQLPQITVKVSASLDGKQANDN
                     GQSQWITNKEVKQDVYKLRHRHDAVLTGRRTVELDDPQYTTRIQDGKNPIKVILSKSG
                     NIHFNQQIYQDESTPIWIYTENPNLTTNQTHIEIIYLKSCDLTTILHNLYKRGVGTLL
                     VEAGPTTTSEFLQSNYIDEFILYYAPKLIGGSGNYQFYQTNDVIEIPDANQFEIVHSE
                     LLNQNVKLTLRKK"
     regulatory      complement(5798..5933)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(6152..7654)
                     /locus_tag="POB55_001946"
     CDS             complement(6152..7654)
                     /locus_tag="POB55_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HDI6500906.1"
                     /translation="MRVAIIGMGTAGVSVLRELVKHPKFNQLDIDLYDDKVNMGQGVP
                     FQNDSSELLINMPSKKMSLNLDDETEFWKWYKQQTDFNFDEPAYLPRFVFGHYMKSYL
                     SMFTKKYPNISTNYNKVQEIYTNSNIDETNLTYYICTTNSEQSWQAYDYVFLTCGTFA
                     YHDPYNLKGKKGYIATPYPTYNTLDEVNELDDIAIIGTGLASLDVVRYVAAHHPKLPI
                     TMTSRSAHLPSVRGTMIDVTFKYLTKDKLNDIKKHHFGNAPLDTLVSLFLKECAEYDI
                     DFKKLVYRRTGNHIADLKYDLARPTEMGIFQSMIEHLKENLNWIWNSLSIEDQHQFNQ
                     KYSKMIQLNSNPMPPRTAELIIELIENKSLILKKDLEDVKHDGKLYYFSYKNQESVDI
                     YNVVINATGAKSHLNELDQDDQLIKNLENRQIVQAHPMGGIQIIPETNQVISPRFGTL
                     TNMIAIGQMTNGVNKLRNGVKMIVEQVAHKVSQLYDALESNEQQQRSDNQ"
BASE COUNT         2425 a         1414 c         1076 g         2744 t
ORIGIN      
        1 atttttcaag atatgatttg tataaatcaa aacatttctt acaaatcaaa tgtgtataaa
       61 atgtgatata cattctcaac cttaaaaaag ttctaaaaaa aagatgagca tctactgtct
      121 catcttttta gaatattaaa tttctacaat ttcgtcatct tcaacaataa agcccattgt
      181 attgacgctg ttatttaaga aagtcagaat ataacgcatt acttcatcgc gttctggctc
      241 attgtgaacc tcgtggtaaa aaccttgcca agctttaaaa tataattcag gtgtttgata
      301 tttttcttta aactcatcaa ttgccctagt atcaacaatt aaatccttcg ttccatacat
      361 taatagcgtt ggcattggtt gaatgtcatg aatatgagcc atcgtatctt tcatcgtctc
      421 attaattgta ttataccaat gatacgttgc tttttttaac attaaaccat cgttaactgt
      481 ttcttcaaca atttctaaat tacgtgttaa atcttttggt tctacaccaa cattaaaacg
      541 tgtgtctttt gaaattttac ctatatttga aacaagttta tctttacgat tttttccatt
      601 cttttgaagt tctagcatag gagaaattaa catcatcccc tcgattggca attctacttt
      661 ttcaagtaaa tttaataaaa tcaaaccgcc aagtcctacc cctaatacat aagtaggaat
      721 tttatattca ttagctatct ttaaccagtc tagcaaactt tcgtgatacg tttgaaagtt
      781 ttcaatttgt cctttattag ctcttgaagt ttgaccttga ccaggcaaat ctcccataat
      841 cacatgatag ccatttcttc ttaacatcgt aataacatat gcatatcttc ccgtatgttc
      901 taatatatta tgagcaataa caacgacgcc tttcgcatca ttttcagctt cccacttcca
      961 cattattata ctgccccttt ttcattaatc ttcaataaca taattatagc aaattcacta
     1021 tgtagatttc tatttatagt attattgttg tccatattat tatatataaa tgaaatcaac
     1081 atcaatgata gtgtaattat acataattat ttttgattgt ttttgatgaa aacgctttct
     1141 cgaatatttt tttcatgcta aacttattgt aaacacaagg gtttggagga gtagcaatgg
     1201 cactattaaa gaattttttt atcggattat ctaataatag ttttttaaac aacgcagcaa
     1261 aaaaagtggg cccacgtttg ggcgccaata aagtcgttgc cggaaataca attccagagt
     1321 taattaatac aatcgaatac ttaaatgaca agaatatcgc tgttacagta gacaatttag
     1381 gggaatttgt cggtacagtt gaagaaagta atcatgctaa agaacaaatt ttaacaatta
     1441 tggacgcgct tcatcaacat ggcgtaaagg cacatatgtc tgttaaattg agtcagttag
     1501 gtgcagaatt cgacttagaa ttagcttacc aaaatttaag agagatttta cttaaagcaa
     1561 atacttacaa caatatgcat ataaatattg atactgaaaa atatgctagc ctgcaacaaa
     1621 ttgttcaagt tttagatcga ttaaaaggcg aatttagaaa tgttggtact gtaattcaag
     1681 catatttata cgatagccat gaattagttg ataagtacca agatttacga ttacgtttgg
     1741 ttaaaggtgc atataaagaa aacgaatcaa ttgcatttca atctaaggaa gacgtagatg
     1801 caaattacat caaaataatt gaacaacgtt tgttaaacgc acgcaatttc acttcaattg
     1861 caacacatga ccatcgcatc attaatcatg taaaacaatt tatgaaagaa aatcacattg
     1921 aaaaagatcg tatggaattc caaatgctct atggttttag atcagagtta gcagaagaaa
     1981 tcgcaaatga aggctataat ttcactattt atgtacctta tggcgatgat tggtttgcgt
     2041 attttatgag aagattagca gaacgcccac aaaacctatc tcttgctgta aaagaatttg
     2101 tgaaacctgc tggcttaaaa cgtgttggca taattgcagc tttaggagct acagttatgt
     2161 taggtttaag tacaattaaa aaattatgcc gtaaatagag caagacataa acaataattt
     2221 aggagtctgg aacaataatc aatgttctag gctcctaaat gttatattgg cagttctgaa
     2281 acatttataa atctttgatt atgaactcac aacagtgctc tatgctttta tagattttaa
     2341 taaattagcc atttcaattg cacttactgc tgcttcggca cctttattac cagctttcgt
     2401 acctgctctt tccacagctt gttcaatact ttcagtcgtt aaaataccaa atatgactgg
     2461 tacattagtt tgatcattta ctttagaaac acctttcgcg acttcattac aaacataatc
     2521 ataatgagac gtagcaccgc gaattacgca tcctaatgta attattgcat cataatttcc
     2581 tgatgaggct aattttttag ctactaaagg aatttcaaac gcacctggca caaatgctac
     2641 atcaatattg tcttcattaa catcatgtcg aatcaaagta tcttttgcac cttcaagtaa
     2701 tcttccagtg ataaaatcat taaatcgact aactacgatt gcaactttca aatcttttcc
     2761 aattaattta ccttcaaaat tcatgttaaa atcctcctat attaaatgac ccatttttat
     2821 ttttttcgtt tccatataat catgattatg taccgtttct ggtacgataa cttcaattct
     2881 ttctgcaata tcaatgccat attgttttaa tccctcaaat ttacttggat tattacttaa
     2941 taaattgata tgttcgatgt taaaatattt taaaatctgt gcagcaatat gataatctcg
     3001 caaatcttca tcaaaaccta atgctaaatt tgcagttact gtatcatatc cttgctcaat
     3061 taattcatat gcgcgtaatt tgtttaacaa tcctatgcca cgaccttctt gaggtagata
     3121 aataatcatg ccaccatgtt cattgatata cttcatagac gattcaagtt gagcaccaca
     3181 atcacaacgt tgactatgga aaatatcgcc tgtaaggcac gcagaatgta agcgtacatt
     3241 ttcatgttgt cgaattgcac cttttgtcag tacaactatc tcttcatctg tgtaagtcgc
     3301 tttaaaacca tacatatcaa atgttccgaa atctgtaggc attttcactt ttgccttaaa
     3361 ttcaatttct ggttctaatt ttttacgata ttcaattaaa tcatcaatcg taatcatctt
     3421 taattgatgt ttttctttaa acttttgtaa atcttgtcct ttcgccatcg tgccgtcatc
     3481 attcataatc tcacaaatga caccagcggg cttggcacca gtaagtttag ctaaatcaac
     3541 agccgcttct gtgtgtccat ttctagctaa tacgccttta tcttgtgcta ctaatggaaa
     3601 taaatgacca ggacgattaa aatctttagc ttcactacta ggatcaatga gctttttggc
     3661 agtcaatgta cgttcataag cactaattcc tgttgttgta tctacatgat caatacttac
     3721 tgtaaattgc gtaccaaaga tgtcggagtt atcatcaacc atttgtacca aatccaaacg
     3781 ttgtgcaata tctttagaca ctggtgcgca tattaatccc cttgcttctt tcgccataaa
     3841 attaatggta ttatcgttca tccattcagt gaccgctact aaatcacctt cattttcacg
     3901 attctcatca tctactacaa taattgtttc tccatttttt aaagccatta aagcactgtc
     3961 aatattatcg aattgcatgc tacccctcct aaaaaccaaa tgctcttaat ttatctacag
     4021 ataattggtc tttatcttta tttaaaatat tttcaacata tttaaacaaa acgtctgttt
     4081 ctaaatgtac tttatctcct aattttttgg atgataaaat cgttgaacgc ctcgtttctg
     4141 gaataagatg aatgtcaaaa ctgttatcat gcttatcaaa taccgttaga cttacaccat
     4201 ccacagtaat agacccttgc ttaactaact gattattaat atgttggcta cattgaatcg
     4261 taataatttt ggcattggct gtttcattta tttttgaaac tgttcctagt tcatctacat
     4321 gaccgaggac aaaatgtcca ccaaacctac cgttaccact catggcacgc tctaaattta
     4381 cttctgattg tcgcttaaca tctgctaaat aggttttatt ttcagtgcct ttaattactt
     4441 gaacagtaaa agatgtctga tcaaaatcaa tcactgttaa acatgcacca ttaacactga
     4501 tggaatcacc aatatgcata tctgccgtaa tcttatgtgc ttcgatttca atcgtcctga
     4561 ctgattgacg aatttgaaca cttttaacga cacctatttc ttcaacgatg ccagtaaaca
     4621 tgcatcatca cttctttcgt aaagttaatt taacattttg atttaataac tcggaatgaa
     4681 caatttcaaa ttggttcgca tctggtatct caatcacatc atttgtttga taaaattgat
     4741 aatttccaga tccgccaatt aatttcgggg cataatagag aataaattca tctatataat
     4801 tagattggag aaattctgaa gtagtggttg gacctgcctc gactagcaaa gttccaactc
     4861 ctcttttata taaattgtga agaattgttg ttaaatcgca agacttcaag taaataattt
     4921 caatatgtgt ttgattggtt gttaaatttg gattttcagt atatatccaa attggtgttg
     4981 attcatcttg ataaatttgc tgattaaaat gaatattccc agacttagac aatattactt
     5041 ttatagggtt ttttccatct tgaatacgtg tagtatattg tggatcatct aattcaactg
     5101 tacgtcttcc agttaacact gcgtcgtgtc gatgtcttaa cttatagaca tcttgtttaa
     5161 cctctttgtt agtaatccat tgactttgtc cattatcatt cgcttgttta ccatctaaac
     5221 ttgcagatac tttcactgta atttgtggca gttgctttgc ttttgcttta aaaaagtctt
     5281 ggtataattg tgatgcccgt tcatcatcaa cgcattcaac ctcaataccg tgagcccgta
     5341 acgtctcatc accatgtgtg tctaacgaat tgtcttttgt tgcgtaaact actttagtta
     5401 tcttacaatc aataattttg ttaacacagg gtggtgttga accaaaatga ctacatggct
     5461 ctaacgtaat ataaatcgtc gcaccttcag cattttgttg tgccatatca agtgcttgaa
     5521 cctccgcatg cttgtcacct tttctcaagt gtgcaccaat accaacaatc ctaccttctt
     5581 taactacaac agcgccaacg ggtggattaa cacctgtttg accttgtacc atatttgcaa
     5641 gttgaatcgc ataatccata aattgactca aatgatcacc tctataaaca aaaatcctca
     5701 catcatgaat taagatgcaa ggagaaaaat ttatcgttaa ataagcctat ttgtacacat
     5761 ttttacaaat acgctacatt atctttgtcg ataattaaca ttctttctcc catccagact
     5821 ttaactgtcg gctctagaat ctcactagat cagccactaa tatgaaacat attagcaggt
     5881 cgcaggcttt atttactgcc ggttgggaat ttcaccctgc cccgaaagaa ttatatatga
     5941 aattgttata gattatttga gtacgtagta tgtcaactac atttaaaatg atactatatg
     6001 ttttctgaaa aaacaattaa tgacggtttt aatttaatat aatctgagta ctataggcat
     6061 ctcattgata tgattcttac taacagacat taaaatcaaa ccttcaattc gtctctatag
     6121 agcgttctct ttattatctt ctagttacaa attattgatt gtcactgcgc tgttgttgct
     6181 cattcgattc taaagcatca tataattgag atactttatg cgcaacttgt tctacaatca
     6241 ttttcacacc atttcgtagt ttattaacac cgtttgtcat ttgacctatc gcaatcatat
     6301 ttgttaatgt tccaaacctt ggactaataa cttgattggt ttccggaatg atttgtatgc
     6361 ctcccattgg gtgtgcttgt acaatttgtc tattttcaag atttttaatt aattgatcat
     6421 cttgatccaa ttcatttaaa tgactttttg cacctgtcgc gttaatgaca acattatata
     6481 tgtctactga ttcttggttt ttgtatgaaa aataatacaa cttgccatca tgtttcacat
     6541 cttctaaatc ttttttcaaa attaaagact tattttctat taattcaata attagttcag
     6601 cagttcttgg aggcattgga tttgaattta attgaatcat ctttgagtat ttttgattaa
     6661 attgatgttg gtcttcaata cttaagctat tccatatcca atttaaattc tctttcaaat
     6721 gttcaatcat actttggaaa atgcccattt ctgttggacg cgctaaatca tacttcaaat
     6781 ctgcaatatg atttcctgta cgtctatata ctaatttttt aaaatcaatg tcatattcag
     6841 cacattcttt taaaaataaa gaaactaaag tatcaagcgg tgcattgccg aaatgatgtt
     6901 ttttaatgtc atttaatttg tctttagtta agtacttgaa tgtcacgtct atcattgtac
     6961 ctcttacact tggtaaatga gcagaacgac tcgtcatagt aattggtaat ttcggatgat
     7021 gagcagcaac ataacggaca acatctaaac tggcaaggcc tgtaccaata atcgcaatat
     7081 cgtccagttc atttacttcg tctaacgtat tatatgttgg ataaggcgta gcgatatatc
     7141 cttttttacc ctttaagtta tatggatcat ggtaggcaaa tgtaccacat gttaaaaata
     7201 cataatcgta cgcttgccat gattgttctg aatttgtagt acatatgtaa taagttaaat
     7261 tcgtttcatc gatattagaa tttgtataaa tctcttgaac tttattataa ttagttgata
     7321 tatttggata ttttttcgtg aacatagata aataagattt catataatgt ccgaatacaa
     7381 atctcggtaa atatgcaggt tcatcaaaat taaaatcagt ttgttgttta taccacttcc
     7441 aaaattcagt ctcatcatct aaatttaaac tcatcttttt cgaaggcata ttaattagca
     7501 gctcagaact atcattttga aatggtacgc cctgtcccat atttacttta tcatcgtata
     7561 aatctatatc taattgatta aacttcgggt gcttaactaa ctctctcaat acacttacac
     7621 cagcagttcc catgcctatt attgctacac gcatatatc
//
LOCUS       DAMZKC010000118         7656 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.119, whole genome shotgun sequence.
ACCESSION   DAMZKC010000118 DAMZKC010000000
VERSION     DAMZKC010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7656
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.119"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..560
                     /locus_tag="POB55_001947"
     CDS             <1..560
                     /locus_tag="POB55_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6500907.1"
                     /translation="MAITLCGFVICYGLIFGLFYLLSGSTADYSIVTIVISAIISIFV
                     IILSIVPVIVLASDLFKERISKGVILIVLAIIALVLCNFVSAILWFVSAISILGRKKL
                     VSTADTTTIQKSKGNANQASHKDTCKKELDSQDMMEHPEFKNPTTKNLEGLNEEIHKD
                     EATTKVDSDNTQPPIESKDHVSKKD"
     gene            772..1548
                     /locus_tag="POB55_001948"
     CDS             772..1548
                     /locus_tag="POB55_001948"
                     /EC_number="1.1.1.304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-acetoin forming diacetyl reductase"
                     /protein_id="HDI6500908.1"
                     /translation="MTNNKVALVTGGAQGIGFKIAERLVEDGFKVAVVDFNEEGAKAA
                     ALKLSSDGTKAIAIKADVSNRDDVFNAVRQTAAQFGDFHVMVNNAGLGPTTPIDTITE
                     EQFKTVYGVNVAGVLWGIQAAHEQFKKFNHGGKIINATSQAGVEGNPGLSLYCSTKFA
                     VRGLTQVAAQDLASEGITVNAFAPGIVQTPMMESIAVATAEEAGKPEAWGWEQFTSQI
                     ALGRVSQPEDVSNVVSFLAGKDSDYITGQTIIVDGGMRFR"
     gene            1692..1814
                     /locus_tag="POB55_001949"
     CDS             1692..1814
                     /locus_tag="POB55_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500909.1"
                     /translation="MHQLKALLVLTHPRYYKTSQKHHYLIYLNKNSQSYLILFL"
     gene            1884..2864
                     /locus_tag="POB55_001950"
     CDS             1884..2864
                     /locus_tag="POB55_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HDI6500910.1"
                     /translation="MEDLERVLITGGAGFIGSHLVDDLQQDYDVYVLDNYRTGKRENI
                     KSLADDHVFELDIREYDAVEQIMKTYQFDYVIHLAALVSVAESVEKPILSQEINVVAT
                     LRLLEIIKKYNSHIKRFIFASSAAVYGDLPDLPKSDQSLILPLSPYAIDKYYGERTTL
                     NYCSLYNIPTAVVKFFNVFGPRQDPKSQYSGVISKMFDSFEHNKPFTFFGDGLQTRDF
                     VYVYDVVQSVRLIMEHKDAIGHGYNIGTGTFTNLLEVYRIIGELYGKSVEHEFKEARK
                     GDIKHSYADISNLKALGFVPKYTVETGLKDYFNFEVDNIEEVTAKEVEMS"
     gene            2827..3519
                     /locus_tag="POB55_001951"
     CDS             2827..3519
                     /locus_tag="POB55_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDI6500911.1"
                     /translation="MKKLQLKKWKCRENDIEAVHNNKGYAYQRKLDKLEEVRKSYYPI
                     KRAIDLILSIVLLFLTLPIMVIFAIAIVIDSPGNPIYSQVRVGKMGKLIKIYKLRSMC
                     KNAEKNGAQWADKDDDRITNVGKFIRKTRIDELPQLINVVKGEMSFIGPRPERPEFVE
                     LFSSEVIGFEQRCLVTPGLTGLAQIQGGYDLTPQQKLKYDMKYIHKGSLMMELYISIR
                     TLMVVITGEGSR"
     gene            3729..4895
                     /locus_tag="POB55_001952"
     CDS             3729..4895
                     /locus_tag="POB55_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDI6500912.1"
                     /translation="MKIIYCITKADNGGAQTHLIQLANHFCVHHDVYVIVGNHGPMIE
                     QLDARVNVIIIEHLVGPIDFKQDILAVKVLAQLFSKIKPDVIHLHSSKAGTVGRIAKF
                     ISKSKDTRIVFTAHGWAFTEGVKPAKKFLYLVIEKLMSLITDSIICVSDFDKQLALKY
                     RFNRLKLTTIHNGIADVPAVKQTLKSQSHNNIGKVVGMLPNKQDLQINAPTKHQFVMI
                     ARFAYPKLPQNLIAAIEILKLHNSNHAHFTFIGDGPTLNDCQQQVAQAGLENDVTFLG
                     NVINASHLLSQYDTFILISKHEGLPISIIEAMATGLPVIASHVGGISELVADNGICMM
                     NNQPETIAKVLEKYLIDSDYIKMSNQSRKRYLECFTEEKMIKEVEDVYNGKSTQ"
     gene            4876..6114
                     /locus_tag="POB55_001953"
     CDS             4876..6114
                     /locus_tag="POB55_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HDI6500913.1"
                     /translation="MENQHNSKLLTLLLIGLAVFIQQSSVIAGVNVSIADFITLLILV
                     YLLFFANHLLKANHFLQFFIILYTYRMIITLCLLFFDDLIFITVKEVLASTVKYAFVV
                     IYFYLGMIIFKLGNSKKVIVTSYIISSVTIGLFCIIAGLNKSPLLMKLLYFDEIRSKG
                     LMNDPNYFAMTQIITLVLAYKYIHNYIFKVLACGILLWSLTTTGSKTAFIILIVLAIY
                     FFIKKLFSRNAVSVVSMLVIMLILLCFTFYNINYYLFQLSDLDALPSLDRMASIFEEG
                     FASLNDSGSERSVVWINAISVIKYTLGFGVGLVDYVHIGSQINGILLVAHNTYLQIFV
                     EWGILFGALFIIFMLYLLFELFRFNISGKNVTAIVVMLTMLIYFLTVSFNNSRYVAFI
                     LGIIVFIVQYEKMERDRNEE"
     gene            6104..7534
                     /locus_tag="POB55_001954"
     CDS             6104..7534
                     /locus_tag="POB55_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HDI6500914.1"
                     /translation="MKSDSLKENIIYQGLYQLIRTMTPLITIPIISRAFGPSGVGIVS
                     FSFNIVQYFLMIASVGVQLYFNRVIAKSVNDKRQLSQQFWDIFVSKLFLALTVFAMYM
                     VVITIFIDDYYLIFLLQGIYIIGAALDISWFYAGTEKFKIPSLSNIVASGIVLSVVVI
                     FVKDQSDLSLYVFTIAIVTVLNQLPLFIYLKRYISFVSVNWIHVWQLFRSSLAYLLPN
                     GQLNLYTSISCVVLGLVGTYQQVGIFSNAFNILTVAIIMINTFDLVMIPRITKMSIQQ
                     SHSLTKTLSNNMNIQLILTIPMVFGLIAIMPSFYLWFFGEEFASTVPLMTILAILVLI
                     IPLNMLISRQYLLIVNKIRLYNASITIGAVMNLVLCLVLIYFYGIYGAAIARLITEFI
                     LLIWRFVDITKINVKLNIVSTIQCVIAAVMMFIVLGVVNHYLPPTMYATLLLIAIGIV
                     VYLLLMMTMKNQYVWQILRLLRHKTI"
BASE COUNT         2550 a         1080 c         1405 g         2621 t
ORIGIN      
        1 tcatggctat aactttatgt gggtttgtaa tttgctatgg actaattttc ggccttttct
       61 atttattatc aggtagcaca gctgattatt caatagtaac aatagttata tcggcaataa
      121 tttctatatt tgtaattata ctttcaatcg tacctgtcat cgtattggca tctgacttat
      181 ttaaagaaag gatttcaaaa ggtgtcatat taattgtatt ggctattatc gctttagtat
      241 tatgcaactt tgtatctgca atactctggt ttgtttcagc catatctatt ttaggtagaa
      301 aaaaattagt atctacagca gatactacca ctattcaaaa aagtaaaggg aacgcaaatc
      361 aagcatcaca taaagacacg tgtaaaaagg aacttgatag tcaagacatg atggaacatc
      421 ctgagtttaa aaatcccacg actaaaaacc tagaaggatt aaacgaagaa atacataaag
      481 atgaagctac aactaaagtt gacagtgata acacgcaacc gccgattgaa tcaaaagacc
      541 atgtctcgaa aaaagattga tgacaaacta atcgagagac ttaaaaaaat aatattcaac
      601 ataagaactt ttaaaacgac atttaaacac attgccaatc actaatggta gtgcgtttaa
      661 ctatacctta aatatctgaa tattttgtta aatggagcta cctttgttgt actattcaaa
      721 tgaagaggag taaaatgtaa ttaaaggaaa gtaatttgag gagtgatctt tatgacaaac
      781 aacaaagtag cgttagtaac tggcggagca caagggattg gttttaaaat tgcagaacgt
      841 ttagtggaag atggtttcaa agtagcagtt gttgatttca atgaagaagg ggcaaaagca
      901 gctgcactta agttatcaag tgatggtaca aaagctattg ctatcaaagc agatgtatca
      961 aaccgtgatg atgtatttaa tgcagtaaga caaactgcag ctcaatttgg cgatttccat
     1021 gtcatggtta acaatgccgg ccttggacca acaacaccaa tcgatacaat tactgaagaa
     1081 cagtttaaaa cagtatatgg cgtgaacgtt gcaggtgtgc tatggggtat tcaagccgca
     1141 catgaacaat ttaaaaaatt caatcatggc ggtaaaatta tcaatgcaac atctcaagca
     1201 ggcgttgagg gtaacccagg cttgtcttta tattgcagta caaaattcgc agtgcgaggt
     1261 ttaacacaag tagccgcaca agatttagcg tctgaaggta ttactgtgaa tgcattcgca
     1321 cctggtatcg ttcagacacc aatgatggaa agtatcgcag tggcaacagc cgaagaagca
     1381 ggtaaacctg aagcatgggg ttgggaacaa tttacaagtc agattgcttt gggcagagtt
     1441 tctcaaccag aagatgtttc aaatgtagtg agcttcttag ctggtaaaga ctctgattac
     1501 attactggac aaacaattat tgtagatggt ggtatgagat tccgttaata atcatgcatt
     1561 agcgataaat aaatccttat tgttaagttt aatcacttag cagtaaggat tttttagtgc
     1621 acttagaagg gagtgtattg gtagaaaatt aataagcgaa gttcttaaat gagttatgat
     1681 gtcactgtct aatgcatcag ttgaaagcat tactagtatt aacacaccca agatattata
     1741 aaacatcaca aaaacaccac tatctaattt atctcaataa aaattcacaa agttatctca
     1801 ttttattttt ataaataaaa aataccgata aaaagcttac aatactttat gtttttatga
     1861 tatattttta atgtataaat gaggtggaag atttggaaag agttttgata actggtgggg
     1921 ctggttttat tgggtcgcat ttagtagatg atttacaaca agattatgat gtttatgttc
     1981 tagataacta tagaacaggt aaacgagaaa atattaaaag tttggctgac gatcatgtgt
     2041 ttgaattaga tattcgtgaa tatgatgcag ttgaacaaat catgaagaca tatcaatttg
     2101 attatgttat tcatttagca gcattagtta gtgttgctga gtcggttgag aaacctatct
     2161 tatctcaaga aataaacgtc gtagcaacat taagattgtt agaaatcatt aaaaaatata
     2221 atagtcatat aaaacgtttt atctttgctt cgtcagcagc tgtttatggt gatcttcctg
     2281 atttgcctaa aagtgatcaa tcattaatct taccattatc accatatgca atagataaat
     2341 attacggcga acggacgaca ttaaattatt gttcgttata taacatacca acagcggttg
     2401 ttaaattttt taatgtattt gggccaagac aggatcctaa gtcacaatat tcaggtgtga
     2461 tttcaaagat gttcgattca tttgagcata acaagccatt tacatttttt ggtgacggac
     2521 tgcaaactag agattttgta tatgtatatg atgttgttca atctgtacgc ttaattatgg
     2581 aacacaaaga tgcaattgga cacggttata acattggtac aggcactttt actaatttat
     2641 tagaggttta tcgtattatt ggtgaattat atggaaaatc agtcgagcat gaatttaaag
     2701 aagcacgaaa aggagatatt aagcattctt atgcagatat ttctaactta aaggcattag
     2761 gatttgttcc taaatataca gtagaaacag gtttaaagga ttactttaat tttgaggtag
     2821 ataatattga agaagttaca gctaaagaag tggaaatgtc gtgaaaatga cattgaagct
     2881 gtccataata ataagggtta tgcctatcaa agaaaattag acaaactaga agaagtgaga
     2941 aaaagctatt acccaattaa acgtgcgatt gacttaattt taagcattgt tttattattt
     3001 ttaactttac cgattatggt tatattcgcc attgctatcg tcatagattc gccaggaaac
     3061 cctatttata gtcaggttag agttgggaag atgggtaaat taattaaaat atacaaatta
     3121 cgttcgatgt gcaaaaacgc agagaaaaac ggtgcgcaat gggctgataa agatgatgat
     3181 cgtataacaa atgtcgggaa gtttattcgt aaaacacgca ttgatgaatt accacaacta
     3241 attaatgttg ttaaagggga aatgagtttt attggaccac gcccggaacg tccggaattt
     3301 gtagaattat ttagttcaga agtgataggt ttcgagcaaa gatgtcttgt tacaccaggg
     3361 ttaacaggac ttgcgcaaat tcaaggtgga tatgacttaa caccgcaaca aaaactgaaa
     3421 tatgacatga aatatataca taaaggtagt ttaatgatgg aactatatat atcaattaga
     3481 acattgatgg ttgttattac aggggaaggc tcaaggtagt cttaatttac ttaataagtt
     3541 caaataaaag ttatatttta aagattgtga ccaattgtta cagtataacg aggaatccct
     3601 tgagacagta tcaaatggca ttaagaaata tgtgccatca ttgatttgca tggctataaa
     3661 tactattcat ctgatgagat agccatgtta agaaattgaa agtatagcat taaaggggtt
     3721 tgtaacagtt gaaaattata tattgtatta ctaaagcaga caatggtggt gcacaaacac
     3781 atctcattca acttgccaac catttttgcg tacaccatga tgtttatgtc attgtaggca
     3841 atcatggacc aatgattgaa caactagatg caagagttaa tgtaattatt atcgaacatt
     3901 tagtaggtcc aattgacttt aaacaagata ttttagctgt caaagtgtta gcgcagttat
     3961 tctcgaaaat taaacctgat gttatccatt tacattcttc caaagctgga acggtcggac
     4021 gaattgcgaa gttcatttcg aaatcgaaag acacacgtat agtttttact gcacatggat
     4081 gggcttttac agagggtgtt aaaccagcta aaaaatttct atatttagtt atcgaaaaat
     4141 taatgtcact tattacagat agcattattt gtgtttcaga tttcgataaa cagttagcgt
     4201 taaaatatcg atttaatcga ttgaaattaa ccacaataca taatggtatt gcagatgttc
     4261 ccgctgttaa gcaaacgcta aaaagccaat cacataacaa tattggcaaa gtagttggaa
     4321 tgttgcctaa taaacaagat ttacagatta atgccccgac aaagcatcaa tttgttatga
     4381 ttgcaagatt tgcttatcca aaattgccac aaaatctaat cgcggcaata gagatattga
     4441 aattacataa cagtaatcat gcgcatttta catttatagg cgatggacct acattaaatg
     4501 attgtcagca acaagttgca caagctgggt tagaaaatga tgtcacattt ttgggcaatg
     4561 tcattaatgc gagtcattta ttatcacaat acgatacgtt tattttaata agtaagcatg
     4621 aaggtttgcc aattagcatt atagaagcta tggctacagg tttgcctgtt atagccagtc
     4681 atgttggcgg tatttcagaa ttagtagctg ataatggtat atgtatgatg aacaatcaac
     4741 cagaaactat tgccaaagtc ttggaaaaat atttaataga cagtgattac atcaaaatga
     4801 gtaatcaatc tagaaaacgt tatttagaat gttttactga ggagaaaatg attaaagaag
     4861 tggaagacgt ttataatgga aaatcaacac aatagtaaat tactaacatt gttacttatc
     4921 ggtttagcgg tttttattca gcaatcttcg gttattgccg gtgtgaatgt ttctatagct
     4981 gactttatca cattactaat attagtttat ttactgtttt tcgctaacca tttattaaag
     5041 gcaaatcatt ttttacagtt tttcattatt ttgtatacat atcgtatgat tattacgctt
     5101 tgtttgctat tttttgatga tttgatattt attacggtta aggaagttct tgcatctaca
     5161 gttaaatatg catttgtagt catttatttc tatttaggga tgatcatctt taagttaggt
     5221 aatagcaaaa aagtgatcgt tacctcttat attataagca gtgtgactat aggtctattt
     5281 tgtattatag ctggtttgaa caagtcccct ttactaatga aattgttata ttttgatgaa
     5341 atacgttcaa aaggattaat gaatgaccct aactatttcg cgatgacaca gattattaca
     5401 ttggtacttg cttacaagta tattcataat tacatattca aggtccttgc atgtggtatt
     5461 ttactatggt ctttaactac aacggggtct aagactgcgt ttatcatatt aatcgtctta
     5521 gccatttatt tctttattaa aaagttattt agcagaaatg cggtaagtgt tgtgagtatg
     5581 ttagtgatta tgctgatatt actttgtttt actttttata atatcaacta ctatttattc
     5641 caattaagcg accttgatgc cttaccgtca ttagatcgaa tggcgtctat ttttgaagag
     5701 gggtttgcat cattaaatga tagtggatct gagcgaagtg ttgtatggat aaatgccatt
     5761 tcagtaatta aatatacatt aggttttggt gttggcttag tggattatgt acatattggc
     5821 tcgcaaatta atggtatttt acttgttgcc cataatacat atttgcaaat ttttgtggaa
     5881 tggggcattt tattcggtgc gttatttatc atatttatgc tttatttact gtttgaatta
     5941 tttagattta acatttctgg gaaaaatgta acagcaattg ttgtaatgtt gacgatgctg
     6001 atttactttt taacagtatc atttaataac tcaagatatg tcgcttttat tttaggaatt
     6061 atcgtcttta ttgttcaata tgaaaagatg gaaagggatc gtaatgaaga gtgattcact
     6121 aaaagaaaat attatttatc aagggctata ccaattgatt agaacgatga caccactgat
     6181 tacaataccc attatttcac gtgcatttgg tcccagtggt gtgggtattg tttcattttc
     6241 tttcaatatc gtgcaatact ttttgatgat tgcaagtgtt ggcgttcagt tatattttaa
     6301 tagagttatc gcgaagtccg ttaacgacaa acggcaattg tcacagcagt tttgggatat
     6361 ctttgtcagt aaattatttt tagcgttaac agtttttgcg atgtatatgg tcgtaattac
     6421 tatatttatt gatgattact atcttatttt cctactacaa ggaatctata ttataggtgc
     6481 agcactcgat atttcatggt tttatgctgg aactgaaaag tttaaaattc ctagcctcag
     6541 taatattgtt gcgtctggta ttgtattaag tgtagttgtt atctttgtca aagatcaatc
     6601 agatttatca ttgtatgtat ttactattgc tattgtgacg gtattaaacc aattaccttt
     6661 gtttatctat ttaaaacgat acattagctt tgtttcggtt aattggatac acgtctggca
     6721 attgtttcgt tcgtcattag catacttatt accaaatgga cagctcaact tatatactag
     6781 tatttcttgc gttgttcttg gtttagtagg cacataccaa caagttggta tcttttctaa
     6841 cgcatttaat attttaacgg tcgcaatcat aatgattaat acatttgatc ttgtaatgat
     6901 tccgcgtatt accaaaatgt ctatccagca atcacatagt ttaactaaaa cgttatctaa
     6961 taatatgaat attcaattga tattaacaat acctatggtc tttggtttaa ttgcaattat
     7021 gccatcattt tatttatggt tctttggtga ggaattcgca tcaactgtcc cattgatgac
     7081 cattttagcg atacttgtat taatcattcc tttaaatatg ttgataagca ggcaatattt
     7141 attaatagtg aataaaataa ggttatataa tgcgtcaatt actattggtg cggtaatgaa
     7201 tttagtatta tgtcttgttt tgatatattt ttatggaatt tacggtgcgg ctattgcacg
     7261 tttaattaca gagtttatct tacttatttg gcgatttgtt gatattacta aaatcaatgt
     7321 gaagttgaat attgtaagta cgattcaatg tgtcattgct gccgttatga tgtttattgt
     7381 gcttggtgtg gtcaatcatt atttgccccc tacaatgtac gctacgctgc tattaattgc
     7441 gattggtata gtagtttatc ttttattaat gatgactatg aaaaatcaat acgtatggca
     7501 aatattgagg cttcttcgac ataaaacaat ttaagtatcg gtaacgctat actttagaaa
     7561 tttaagatta agaagaaaag gcaatttctt attgaaaaat ggaagttgtc ttttttaatt
     7621 ctctttaaaa gcgggaaaca aaagcagtta aatgcc
//
LOCUS       DAMZKC010000119         7621 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.120, whole genome shotgun sequence.
ACCESSION   DAMZKC010000119 DAMZKC010000000
VERSION     DAMZKC010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7621
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.120"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            107..1468
                     /locus_tag="POB55_001955"
     CDS             107..1468
                     /locus_tag="POB55_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000190617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HDI6500915.1"
                     /translation="MATGVLEDDIVKEIYGSSKEWVSVEVKLSQSLDPSTLFHLTDNE
                     AGDRFYMRLNDNRTSYFGYKAIQLFKNNSKNKQSIFKDWEKLKHNITFIHPQSEKHHL
                     RVVGGFQFSSHKSDDEWREFGLNHFVLPEVLISTDNNGTFLTYTVKRESFTVEALNDL
                     MDLFNNISDIDVDEQIGEITRNEDIYKDDWRQLVVEAIESINNEEKIVLARRRLIKFD
                     KDISIPYILKQAYSKEKNSYIFLLESQDSIFFSQTPEQLIKVNNKILSTKAVAGTIKR
                     SQDEDEDTKNVEAFLKDNKNLIEHRFVVDSILHDIKPYITELHYDKTPKILKNDHLYH
                     LYTEIKAPLKDDSYISLIDNLHPTPALGGYPKEFAMDFIEQKEFGTRGLYGAPVGYID
                     IYDDCEFIVAIRSMLIKKAQATLFAGCGIVKDSDPDSELAETNLKFTPMMNALGVDMN
                     GKS"
     gene            1455..3128
                     /gene="menD"
                     /locus_tag="POB55_001956"
     CDS             1455..3128
                     /gene="menD"
                     /locus_tag="POB55_001956"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HDI6500916.1"
                     /translation="MGNHKAALTKQVFTFASELYAYGVREVVISPGSRSTPLALAFEA
                     HPNIKTWIHPDERSAAFFAVGLIKGSERPVAILCTSGTAAANYTPAIAESQISRIPLI
                     VLTSDRPHELRSVGAPQAINQVNMFNNYVSYEFDMPIADDSKETINAIYYQMQIASQY
                     LYGPHKGPIHFNLPFRDPLTPDLNATELLTSEMKILPHYQKSIDASALRHILNKKKGL
                     IIVGDMQHQEVDQILTYSTIYDLPILADPLSHLRKFDHPNVICTYDLLFRSGLDLNVD
                     FVIRVGKPVISKKLNQWLKKTDAFQILVQNNDKIDVFPIAPDISYEISANDFFRSLME
                     DTTINRVSWLEKWQRLEKKGRKEIKCYLEQATDESAFVGELIKKTSEKDALFISNSMP
                     IRDVDNLLLNKNIDVYANRGANGIDGIVSTALGMAVHKRITLLIGDLSFYHDMNGLLM
                     SKLNNIQMNIVLLNNDGGGIFSYLPQKESATDYFERLFGTPTGLDFEYTAKLYQFDFK
                     RFNSVSEFKNATLLSETSTIYELITNREDNFKQHQILYQKLSEMIHGTL"
     gene            3115..3918
                     /gene="menH"
                     /locus_tag="POB55_001957"
     CDS             3115..3918
                     /gene="menH"
                     /locus_tag="POB55_001957"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HDI6500917.1"
                     /translation="MAHYNYYEANVETNQVLVLLHGFLSDSRTYYNHIDKYTDICHVI
                     TIDLPGHGEDQSSMDETWNFDYITTLLDRILDKYKDKSITLFGYSMGGRVALYYAING
                     HIPISNLILESTSPGIKEEANQLERRLVDDARAKVLDIAGIELFVNDWEKLPLFQSQL
                     ELPVEIQHQIRQQRLSQSPHKMAKALRDYGTGQMPNLWPRLKEIKVPTLILAGEYDEK
                     FVQIAKKMANLIPNSKCKLISATGHTIHVEDSDEFDTMILGFLKEEQND"
     gene            3911..4732
                     /gene="menB"
                     /locus_tag="POB55_001958"
     CDS             3911..4732
                     /gene="menB"
                     /locus_tag="POB55_001958"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HDI6500918.1"
                     /translation="MTNRQWETLREYDEIKYEFYEGIAKVTINRPEVRNAFTPKTVSE
                     MIDAFSRARDDQNVSVIVLTGEGDLAFCSGGDQKKRGHGGYVGEDQIPRLNVLDLQRL
                     IRIIPKPVIAMVKGYAVGGGNVLNVVCDLTIAADNAIFGQTGPKVGSFDAGYGSGYLA
                     RIVGHKKAREIWYLCRQYNAQEALDMGLVNTVVPLEKVEDETVQWCKEIMKHSPTALR
                     FLKAAMNADTDGLAGLQQMAGDATLLYYTTDEAKEGRDAFKEKRDPDFDQFPKFP"
     gene            complement(4970..5299)
                     /gene="sspC"
                     /locus_tag="POB55_001959"
     CDS             complement(4970..5299)
                     /gene="sspC"
                     /locus_tag="POB55_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphostatin B"
                     /protein_id="HDI6500919.1"
                     /translation="MYQLQFINLVYDTTKLTHLEQTNINLFIGNWSNHQLQKSICIRH
                     GDDTSHNQYHILFIDTAHQRIKFSSIDNEEIIYILDYDDTQHILMQTSSKQGIGTSRP
                     IVYERLV"
     gene            complement(5337..6518)
                     /gene="sspB"
                     /locus_tag="POB55_001960"
     CDS             complement(5337..6518)
                     /gene="sspB"
                     /locus_tag="POB55_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease staphopain B"
                     /protein_id="HDI6500920.1"
                     /translation="MNSSYKSRVFNIISIIMVSMLILSLGAFANNNKAKADSHSKQLE
                     INVKSDKVPQKVKDLAQQQFAGYAKALDKQSNAKTGKYELGEAFKIYKFNGEEDNSYY
                     YPVIKDGKIVYTLTLSPKNKDDLNKSKEDMNYSVKISNFIAKDLDQIKDKNSNITVLT
                     DEKGFYFEEDGKVRLVKATPLPGNVKEKESAKTVSSKLKQELKNTVTPTKVEENEAIQ
                     EDQVQYENTLKNFKIREQQFDNSWCAGFSMAALLNATKNTDTYNAHDIMRTLYPEVSE
                     QDLPNCSTFPNQMIEYGKSQGRDIHYQEGVPSYEQVDQLTKDNVGIMILAQSVSQNPN
                     DPHLGHALAVVGNAKINDQEKLIYWNPWDTELSIQDADSSLLHLSFNRDYNWYGSMIG
                     Y"
     gene            complement(6600..7601)
                     /gene="sspA"
                     /locus_tag="POB55_001961"
     CDS             complement(6600..7601)
                     /gene="sspA"
                     /locus_tag="POB55_001961"
                     /EC_number="3.4.21.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-specific serine endopeptidase SspA"
                     /protein_id="HDI6500921.1"
                     /translation="MKGKFLKVSSLFVATLTTATLVSSPAANALSSKAMDNHPQQSQS
                     SKQQTPKIQKGGNLKPLEQREHANVILPNNDRHQITDTTNGHYAPVTYIQVEAPTGTF
                     IASGVVVGKDTLLTNKHVVDATHGDPHALKAFPSAINQDNYPNGGFTAEQITKYSGEG
                     DLAIVKFSPNEQNKHIGEVVKPATMSNNAETQVNQNITVTGYPGDKPVATMWESKGKI
                     TYLKGEAMQYDLSTTGGNSGSPVFNEKNEVIGIHWGGVPNEFNGAVFINENVRNFLKQ
                     NIEDIHFANDDQPNNPDNPDNPNNPDNPNNPDNPNNPNNPDNPDNGDNNNSDNPDAA"
BASE COUNT         2521 a         1111 c         1419 g         2570 t
ORIGIN      
        1 acgtgaaaaa ttgcaagata agtttaagca attcttgtaa cacaattatc caaaaatgat
       61 acaatataat aatgagtgaa aaaagacgaa gaaagaagtg aaatggatgg ctacgggcgt
      121 attagaggac gatattgtca aagagatata tggcagctca aaggaatggg tttcagttga
      181 agtgaaatta tcacagtcac tagacccgag cacattattt catctcactg acaatgaggc
      241 aggagatcgc ttttatatgc gtttgaatga taatcgaacg tcatattttg gctacaaagc
      301 aattcaatta ttcaaaaata attctaaaaa taaacaatct atttttaaag actgggaaaa
      361 attaaaacat aacatcacat ttatacatcc gcaatctgag aaacatcatc ttcgagttgt
      421 tggaggattc caattttcga gtcataaatc agatgatgaa tggcgagagt ttggactaaa
      481 tcattttgta ttacctgaag ttttaatttc aactgataat aatgggacat ttttaactta
      541 tacagttaaa agggaaagtt ttactgttga ggcattgaac gatttaatgg atttgttcaa
      601 caatatatcg gacatagatg tggacgagca aattggggaa attactagaa atgaagatat
      661 ttataaagat gactggcgtc aacttgtagt agaagctata gaatctatta ataatgaaga
      721 aaaaattgta ctagcacgta gacggttaat aaagttcgat aaagatatca gtattccata
      781 tattctaaag caagcatatt ctaaagaaaa aaacagttat atattcttgt tagaatcaca
      841 agattctata ttcttttcac aaacacctga acaattaata aaggtcaata ataaaatact
      901 atcgactaaa gctgtagcag gtacaattaa acgttcacaa gatgaggacg aagatacaaa
      961 aaatgttgaa gcatttttaa aagataataa aaacttaatc gaacatcgat ttgttgttga
     1021 cagtatttta catgatatta aaccttatat cactgaatta cattatgata agacgcctaa
     1081 aattctaaaa aatgatcatt tatatcactt gtacactgaa ataaaggcgc cactgaagga
     1141 tgattcgtat attagtttaa ttgataattt acatccaaca cctgctttag gtggctatcc
     1201 aaaagaattt gcgatggatt ttattgaaca gaaagaattt ggtacacgag gattatatgg
     1261 tgcgccggtt ggctatatag atatatatga tgattgtgaa tttattgttg caattcgttc
     1321 gatgcttatt aagaaagcac aagcaacttt atttgctggg tgtggcattg ttaaagattc
     1381 tgatccagat agtgaattgg cagaaacgaa ccttaagttc acacctatga tgaatgcatt
     1441 aggagtcgat atgaatggga aatcataaag cagctttaac gaagcaagtt tttacatttg
     1501 catctgagtt atatgcgtac ggcgtaaggg aagtagttat cagtccggga tcacgctcaa
     1561 cgccacttgc acttgcattt gaagcacatc caaatattaa aacatggata caccccgatg
     1621 agcgaagtgc agcatttttt gcagttgggt taattaaagg tagtgaaaga cctgtcgcta
     1681 tattatgtac gtcaggtaca gcagcagcga attatacgcc tgcaattgct gaaagccaaa
     1741 ttagtagaat tccattaatc gttttaacaa gtgaccgtcc gcatgaatta agaagtgtag
     1801 gcgcaccaca agcgattaat caagtaaata tgtttaataa ttatgtaagt tatgagttcg
     1861 atatgcctat tgcggatgat agtaaagaga ccattaatgc aatttattat caaatgcaaa
     1921 ttgctagtca atatttatat ggaccacata aagggccaat tcattttaac ttgccattta
     1981 gagatccgtt aacacctgat ttgaatgcaa cagaattgtt aacttctgag atgaagattt
     2041 taccgcacta tcaaaaaagt atagatgcat cggcattaag acacatttta aataagaaaa
     2101 aaggtttaat tattgtaggg gatatgcagc accaagaagt tgatcaaata ctaacgtatt
     2161 caacgatata tgatttgcct attttagctg atcctttaag tcatttaaga aaatttgatc
     2221 atccgaatgt tatctgtaca tatgatttgc tgtttagaag cggcttagac ttaaatgtgg
     2281 atttcgtaat tcgtgttggg aaaccagtga tttctaaaaa gttgaatcaa tggttaaaga
     2341 aaactgatgc atttcaaata ttagtgcaaa acaatgataa gattgatgtc tttccgatag
     2401 caccagatat ttcatatgag atttctgcga atgatttctt taggtcatta atggaagaca
     2461 cgaccatcaa tcgcgtaagt tggttagaaa aatggcaacg cttagagaaa aaagggcgta
     2521 aagaaattaa atgttatttg gaacaagcta cagatgagag tgcattcgtt ggtgaattga
     2581 ttaagaaaac atctgaaaaa gatgcattat ttattagtaa tagtatgcct atcagagatg
     2641 tagataactt gttattgaat aaaaatatag atgtctatgc gaatcgtggt gcgaatggta
     2701 ttgatggtat cgtttcaact gcactgggta tggctgtgca taaacgaata acattattga
     2761 taggtgattt atcattttat catgatatga atggactatt aatgtcaaaa ttaaataata
     2821 ttcagatgaa tattgtatta ttgaacaacg atggtggcgg tattttttca tatttaccac
     2881 aaaaagaaag tgcaactgac tattttgaac ggttgtttgg cacaccgacg ggattggatt
     2941 tcgagtatac agctaagtta tatcaattcg attttaaacg ttttaacagt gtttcagaat
     3001 ttaaaaatgc cacattgtta tctgaaactt cgacgattta tgaattgata acgaatcgcg
     3061 aagataactt taaacagcat caaattttat atcagaaatt gagtgaaatg attcatggca
     3121 cattataatt actatgaagc aaacgttgag accaatcaag ttttagtatt actgcatggt
     3181 tttcttagcg acagtcgtac ttattataat cacatcgata aatatactga tatctgtcat
     3241 gtcatcacta tagacttacc aggacatggc gaagatcagt cttcaatgga tgaaacgtgg
     3301 aattttgatt atattacgac gttgttagac cgaattttag ataaatataa agataaatca
     3361 ataacattgt ttggatattc aatgggtgga cgtgttgcat tatattatgc aattaatggt
     3421 cacatcccta tatctaattt gatattagaa agtacgtcac caggtattaa agaagaagca
     3481 aatcaattgg aacgccgtct tgttgatgat gcacgtgcta aagtattaga catagcaggt
     3541 attgaattat ttgttaatga ttgggaaaag ttgccattat ttcaatcgca actagaatta
     3601 ccagttgaaa tacaacatca aataagacaa caacgattgt cccaatcgcc acataaaatg
     3661 gccaaagcat taagagatta tggtacaggt caaatgccaa acttatggcc gcgcctgaaa
     3721 gaaattaaag taccaacatt aatattagct ggagaatatg atgaaaaatt tgtacagatt
     3781 gcgaaaaaaa tggcaaattt aattcctaat agtaaatgta aattaatttc tgctacaggt
     3841 catacaattc atgtggaaga tagtgatgaa tttgatacaa tgatattagg atttttaaag
     3901 gaggagcaaa atgactaaca gacaatggga aacacttaga gaatatgatg aaatcaaata
     3961 tgaattttat gaagggattg ctaaggtaac aataaatcgc cctgaagtac gcaatgcgtt
     4021 tacacctaaa acagtttctg aaatgattga cgcattttca cgtgcacgtg atgatcaaaa
     4081 cgtttcagtt atcgtattaa ctggtgaagg tgatttagca ttctgttctg gtggtgacca
     4141 gaagaaacgt ggacatggtg gttatgtagg tgaagaccaa atccctcgct taaatgtatt
     4201 agatttacag cgtttaattc gtattattcc aaaaccggtt atcgcgatgg taaaaggtta
     4261 tgctgtaggt ggcggtaatg tactaaatgt tgtttgtgac ttaacgattg ctgctgataa
     4321 tgctattttt ggacaaactg gtcctaaagt aggttcattt gatgcgggtt atggttcagg
     4381 atatttagca cgtatcgttg gacataagaa agcacgtgaa atttggtact tatgtcgtca
     4441 atacaatgca caagaagctt tagatatggg tctagtaaat acagtggtac ctttagagaa
     4501 agttgaagat gaaactgtgc aatggtgtaa agagattatg aaacactcac caacagcgtt
     4561 acgattcctt aaagcagcta tgaatgctga cacagatggt ttagctggtt tacaacaaat
     4621 ggctggggat gcaacactgc tttattacac aactgatgaa gcgaaagaag gccgtgatgc
     4681 gtttaaagaa aaacgtgatc ctgacttcga tcaattccct aaattcccat aagttatttt
     4741 gaatatgata ttaagtcact tgcgtcgttt attagcgacg ataggtggct tttttatttt
     4801 ataagaattt aaaaggggct tttaattaat tatcataaat ataagatagt ttgagaaaat
     4861 taatagagaa aaaataaatg ttaaagatta ttattacaat caaaaaacga gctggtgaga
     4921 aattgaattc tcgccaaccc gctttatgaa gtgaaatcat ttaaattagt tatactaagc
     4981 gctcataaac gattgggcgc gaagtgccaa taccttgttt ggatgacgtt tgcatgagga
     5041 tatgctgtgt atcatcataa tcaagaatat aaatgatttc ttcattatca atagatgaaa
     5101 atttaatgcg ttgatgtgcc gtatctataa aaagaatatg atattgattg tgacttgtat
     5161 catcgccatg acgtatacaa attgattttt gtaattgatg attactccaa ttaccaatga
     5221 ataaattgat attggtttgt tctagatgtg tgagtttggt tgtgtcgtaa actaaattta
     5281 taaattgtag ttgatacata gttttacctg cctttaatca atatctatat tactttttag
     5341 taacctatca ttgaaccata ccagttataa tcacgattga atgataaatg taataggctt
     5401 gaatctgcat cttggattga taattctgta tcccaaggat tccagtaaat aagtttttct
     5461 tggtcattaa ttttagcatt accaacaact gctagggcat gtcctaaatg tggatcatta
     5521 gggttttgag atacactttg tgcaagaatc ataattccta cattatcttt tgtaagttga
     5581 tcaacttgtt catatgatgg tacgccttct tgataatgaa tatctctgcc ttgtgattta
     5641 ccgtattcaa tcatttgatt agggaatgtt gagcaattag gaaggtcttg ctcacttact
     5701 tcagggtata atgtacgcat aatatcatgt gcattataag tgtctgtatt tttagttgca
     5761 tttaataatg ctgccatact aaatcctgca caccatgagt tatcgaattg ttgttctcta
     5821 attttgaagt tttttaatgt attttcatat tgaacttgat cttcttgtat cgcttcgttt
     5881 tcttcaactt tagtaggtgt tactgtattt tttaactctt gtttcaattt tgatgaaact
     5941 gttttagcac tttctttttc ttttacatta ccaggtagtg gcgtagcttt tactaatcta
     6001 actttgccat cttcttcaaa ataaaatcct ttttcatcag taagaacagt gatatttgaa
     6061 tttttatctt taatttggtc taaatcttta gcgatgaagt ttgaaatttt aacactgtaa
     6121 ttcatgtctt cttttgattt gtttaaatca tctttatttt tagggctaag tgttaaagta
     6181 taaacaattt taccgtcttt tataactgga taatagtaac tattatcttc ttcaccatta
     6241 aatttataaa ttttaaaagc ttcgccaagt tcatatttac cagtttttgc attactttgt
     6301 ttatctaatg ctttagcata accagcaaat tgttgttgtg ctagatcttt tactttttga
     6361 ggtactttgt cactcttaac attaatttct agctgtttag agtgtgaatc ggctttggct
     6421 ttattgttat tagcaaatgc gcctagtgat aaaataagca ttgaaaccat tatgatgctt
     6481 ataatattga atactctaga tttatatgaa ctattcatat aataccctcc atttaaatca
     6541 aaatattttg cgttgctaag actttgcttc tatttactaa atctaaatta agatgaagtt
     6601 tatgcagcgt cagggttgtc tgaattattg ttatcgccat tgtctggatt atcagggttg
     6661 ttagggttgt tagggttatc aggattgtta gggttatcag gattgttagg gttatcagga
     6721 ttatctgggt tattaggttg gtcatcgttg gcaaaatgga tatcttcaat attttgtttt
     6781 aagaagttgc gtacattttc attaataaat accgcaccat taaattcatt tggtacaccg
     6841 ccccaatgaa ttccgatcac ttcatttttt tcattaaata caggtgaacc tgagttacca
     6901 ccagttgtac ttaaatcata ttgcatagct tcgcctttga ggtaagtgat ttttccttta
     6961 ctttcccaca ttgttgctac aggtttatca ccaggatatc ctgttacagt aatattttgg
     7021 ttaacttgtg tttcagcatt attactcatt gttgctggtt taactacttc accaatatgt
     7081 ttgttttgct cattagggga gaatttaact attgctaaat caccttcgcc tgaatattta
     7141 gtgatttgtt cagcagtgaa accaccattt ggataattgt cttggttaat tgcagaaggg
     7201 aatgctttta aagcatgagg atcaccgtgc gtagcatcta cgacgtgttt atttgttaaa
     7261 agagtatctt tacctacaac tacaccggaa gcaataaatg taccagtagg tgcttcaact
     7321 tgaatataag ttacgggtgc ataatgacca ttcgttgtat ctgtgatttg gtgacgatcg
     7381 ttatttggta atataacatt tgcgtgttca cgttgttcta atggtttaag gttaccgcct
     7441 ttttgaatct taggtgtttg ctgtttgctt gactgtgatt gttgcggatg attgtccata
     7501 gcctttgaag ataacgcgtt tgctgctgga gaactcacaa gtgtcgctgt tgtcaaagtt
     7561 gcaacgaata aagaactaac ttttaaaaat ttacctttca tctaaaaacc tccaaaaaat
     7621 t
//
LOCUS       DAMZKC010000120         7526 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.121, whole genome shotgun sequence.
ACCESSION   DAMZKC010000120 DAMZKC010000000
VERSION     DAMZKC010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7526
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.121"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            5..646
                     /gene="capA"
                     /locus_tag="POB55_001962"
     CDS             5..646
                     /gene="capA"
                     /locus_tag="POB55_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide type 5/8 biosynthesis
                     protein CapA"
                     /protein_id="HDI6500922.1"
                     /translation="MKEVLQKNLKILIILPLLFLIISAIVTFFVLSPKYQANTQILVN
                     QTKGDNPQFMAQEVQSNIQLVNTYKEIVKSPRILDEVSKDLNDKYSPSKLSSMLTITN
                     QENTQLINIQVKSGHKQDSEKIANSFAKVTSKQIPKIMSVDNVSILSKADGTAVKVAP
                     KTVVNLIGAFFLGLVVALIYIFFKVIFDKRIKDEEDVEKELGLPVLGSIQKFN"
     gene            662..1348
                     /gene="cap8B"
                     /locus_tag="POB55_001963"
     CDS             662..1348
                     /gene="cap8B"
                     /locus_tag="POB55_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8B"
                     /protein_id="HDI6500923.1"
                     /translation="MSKKENTTTTLFVYEKPKSTISEKFRGIRSNIMFSKANGEVKRL
                     LVTSEKPGAGKSTVVSNVAITYAQAGYKTLVIDGDMRKPTQNYIFNEQNNNGLSSLII
                     GRTTMSEAITSTEIENLDLLTAGPVPPNPSELIASERFKELVDLFNKRYDIIIVDTPP
                     VNTVTDAQLYARAIKDSLLVIDSEKNDKNEVKKAKALMEKAGSNILGVILNKTKVDKS
                     SSYYHYYGDE"
     gene            1351..2115
                     /gene="cap8C"
                     /locus_tag="POB55_001964"
     CDS             1351..2115
                     /gene="cap8C"
                     /locus_tag="POB55_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8C"
                     /protein_id="HDI6500924.1"
                     /translation="MIDIHNHILPNIDDGPTNETEMLDLLKQATTQGVTEIIVTSHHL
                     HPRYTTPIEKVKSCLNHIESLEEVQALNLKFYYGQEIRITDQILNDIDRKVITGINDS
                     RYLLIEFPSNEVPHYTDQLFFELQSKGFVPIIAHPERNKAISQNLDILYDLINKGALS
                     QVTTASLAGISGRKIRKLAIQMIENNLTHFIGSDAHNTEIRPFLMKDLFNDKKLRDYY
                     EDMNGFISNAKLVVDDKKIPKRMPQQDYKQKRWFGL"
     gene            2135..3958
                     /gene="cap8D"
                     /locus_tag="POB55_001965"
     CDS             2135..3958
                     /gene="cap8D"
                     /locus_tag="POB55_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8D"
                     /protein_id="HDI6500925.1"
                     /translation="MAHLSVKLRLLILALIDSLIVTFSVFVSYYILEPYFKTYSVKLL
                     ILAAISLFISHHISAFIFNMYHRAWEYASVSELILIVKAVTTSIVITMVVVTIVTGNR
                     PFFRLYLITWMMHLILIGGSRLFWRIYRKYLGGKSFNKKPTLVVGAGQAGSMLIRQML
                     KSDEMKLEPVLAVDDDEHKRNITITEGVKVQGKIADIPELVRKYKIKKIIIAIPTIGQ
                     ERLKEINNICHMDGVELLKMPNIEDVMSGELEVNQLKKVEVEDLLGRDPVELDMDMIS
                     NELTNKTILVTGAGGSIGSEICRQVCNFYPERIILLGHGENSIYLINRELRNRFGKNV
                     DIVPIIADVQNRARMFEIMETYKPYAVYHAAAHKHVPLMEENPEEAVRNNILGTKNTA
                     EAAKNAEVKKFVMISTDKAVNPPNVMGASKRIAEMIIQSLNDETHRTNFVAVRFGNVL
                     GSRGSVIPLFKSQIEEGGPVTVTHPEMTRYFMTIPEASRLVLQAGALAEGGEVFVLDM
                     GEPVKIVDLARNLIKLSGKKEDDIRITYTGIRPGEKMFEELMNKDEVHPEQVFEKIYR
                     GKVQHMKCNEVEAIIQDIVNDFSKEKIINYANGKKGDNYVR"
     gene            3948..4976
                     /gene="cap8E"
                     /locus_tag="POB55_001966"
     CDS             3948..4976
                     /gene="cap8E"
                     /locus_tag="POB55_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8E"
                     /protein_id="HDI6500926.1"
                     /translation="MFDDKILLITGGTGSFGNAVMKRFLDSNIKEIRIFSRDEKKQDD
                     IRKKYNNSKLKFYIGDVRDSQSVETAMRDVDYVFHAAALKQVPSCEFFPVEAVKTNII
                     GTENVLQSAIHQNVKKVICLSTDKAAYPINAMGISKAMMEKVFVAKSRNIRSEQTLIC
                     GTRYGNVMASRGSVIPLFIDKIKAGEPLTITDPDMTRFLMSLEDAVELVVHAFKHAET
                     GDIMVQKAPSSTVGDLATALLELFEADNAIEIIGTRHGEKKAETLLTREEYAQCEDMG
                     DYFRVPADSRDLNYSNYVETGNEKITQSYEYNSDNTHILTVEEIKEKLLTLEYVRNEL
                     NDYKASMR"
     gene            4989..6098
                     /gene="cap8F"
                     /locus_tag="POB55_001967"
     CDS             4989..6098
                     /gene="cap8F"
                     /locus_tag="POB55_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8F"
                     /protein_id="HDI6500927.1"
                     /translation="MNIVITGAKGFVGKNLKADLTSTTDHHIFEVHRQTKEEELESAL
                     LKADFVVHLAGVNRPEHDKEFSLGNVSYLDHVLDILTRNTKKPAILLSSSIQATQDNP
                     YGESKLQGEQLLREYAEEYGNTVYIYRWPNLFGKWCKPNYNSVIATFCYKIARNEEIQ
                     VNDRNVELTLNYVDDIVAEIKRAIEGTPTIENGVPTVPNVFKVTLGEIVDLLYKFKQS
                     RLDRTLPKLDNLFEKDLYSTYLSYLPSTDFSYPLLMNVDDRGSFTEFIKTPDRGQVSV
                     NISKPGITKGNHWHHTKNEKFLVVSGKGVIRFRHVNDDEIIEYYVSGDKLEVVDIPVG
                     YTHNIENLGDTDMVTIMWVNEMFDPNQPDTYFLEV"
     gene            6102..7226
                     /gene="cap8G"
                     /locus_tag="POB55_001968"
     CDS             6102..7226
                     /gene="cap8G"
                     /locus_tag="POB55_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8G"
                     /protein_id="HDI6500928.1"
                     /translation="MEKLKLMTIVGTRPEIIRLSSTIKACDQYFNQILVHTGQNYDYT
                     LNQIFFDDLELRQPDHYLEAVGSNLGETMGNIIAKTYDVLLREQPDALLILGDTNSCL
                     AAVSAKRLKIPVFHMEAGNRCFDQNVPEEINRKIVDHVSDVNLPYTEHSRRYLLDEGF
                     NKANIFVTGSPMTEVIEAHRDKINHSDVLNKLGLEPQQYILVSAHREENIDNEKNFKS
                     LMNAINDIAKKYKMPVIYSMHPRSWKKIEESKFEFDPLVKKLKPFGFFDYNALQKDAF
                     VVLSDSGTLSEESSILKFPGVLIRTSTERPEVLDKGTVIVGGITYNNLIQSVELAREM
                     QNNNEPMIDAIDYKDTNVSTKVVKIIQSYKDIINRNTWRK"
     gene            7229..>7526
                     /gene="cap8H"
                     /locus_tag="POB55_001969"
     CDS             7229..>7526
                     /gene="cap8H"
                     /locus_tag="POB55_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8H"
                     /protein_id="HDI6500929.1"
                     /translation="MRIAILGATNIKHMSLLSHYLNHIDLNINEVDIIYTDKYDIEEH
                     IQGINNYYKYKVDIKEDWTFIKKAIAYYRFRPYAMKILKENRYDFVIVWGSYTGH"
BASE COUNT         2758 a         1063 c         1472 g         2233 t
ORIGIN      
        1 aaaaattaaa gaagtattac aaaaaaactt gaagatttta attattttac cgctattatt
       61 tttaattatt agcgctattg ttacattttt cgtcttatca cctaaatatc aagctaatac
      121 tcaaatctta gtgaatcaaa ctaagggtga caatcctcag tttatggcac aagaggttca
      181 aagtaatatt caacttgtaa atacgtataa agaaattgtt aaaagtccta gaattttaga
      241 tgaggtgtca aaggacttaa atgataagta ttcaccatct aaattgtcga gtatgttgac
      301 aattacaaac caagaaaata cgcaacttat caacatccaa gttaaaagtg gtcataaaca
      361 agattcggaa aaaattgcga atagcttcgc taaagttaca agtaaacaaa ttccgaagat
      421 tatgagtgtg gataacgtat caattttatc taaagcagac ggtacagcag ttaaagtcgc
      481 accaaaaact gtagtgaatc taatcggtgc attcttttta ggattagttg tcgcgcttat
      541 atatatcttc ttcaaagtaa ttttcgataa gcgaattaaa gatgaagaag atgtagagaa
      601 agaattagga ttgcctgtat tgggttcaat tcaaaaattt aattaaggat ggttgctact
      661 tatgtcaaaa aaggaaaata cgacaacaac actatttgtg tatgaaaaac caaaatcaac
      721 aattagtgaa aagtttcgag gtatacgttc aaacatcatg ttttcaaaag caaatggtga
      781 agtaaagcgc ttattggtta cttctgaaaa gcctggtgca ggtaaaagta cagttgtatc
      841 gaatgtagcg attacttatg cacaagcagg ctataagaca ttagttattg atggcgatat
      901 gcgtaagcca acacaaaact atatttttaa tgagcaaaat aataatggac tatcaagctt
      961 aatcattggt cgaacgacta tgtcagaagc aattacgtcg acagaaattg aaaatttaga
     1021 tttgctaaca gctggccctg tacctccaaa tccatctgag ttaatagctt cagaaaggtt
     1081 taaagaatta gttgatctgt ttaataaacg ttacgacatt attattgtcg atacaccgcc
     1141 agttaatact gtgactgatg cacaactata tgcgcgtgct attaaagata gtttgttagt
     1201 aattgatagt gaaaaaaatg ataaaaatga agttaaaaaa gcaaaagcac ttatggaaaa
     1261 agcaggcagt aacattctag gtgtcatttt gaacaagaca aaggtcgata aatcttctag
     1321 ttattatcac tattatggag atgaataagt atgattgata ttcataacca tatattgcct
     1381 aatatcgatg acggtccgac aaatgaaaca gagatgctgg atcttttaaa acaagcgaca
     1441 acacaaggtg ttacagaaat cattgtaaca tcacatcact tacatcctcg atataccaca
     1501 cctatagaaa aagtgaaatc atgtttaaac catattgaaa gcttagagga agtacaagca
     1561 ctaaatctaa agttttatta tggtcaggaa ataagaatta ccgatcaaat ccttaatgat
     1621 attgatcgaa aagttattac cggtattaat gattcacgct atttactaat agaatttcca
     1681 tcaaatgaag ttccacacta tactgatcaa ttatttttcg aattacagag taaaggcttt
     1741 gtaccgatta ttgcacatcc agagcggaat aaagcaataa gtcaaaacct tgacatacta
     1801 tacgatttaa ttaacaaagg tgctttaagt caagtgacaa cggcgtcatt agcgggtatt
     1861 tccggtagaa aaattagaaa attagcaatt caaatgattg aaaacaatct gacacatttc
     1921 atcggttcag atgcgcataa cacagaaatc agaccgttct taatgaaaga cttatttaat
     1981 gataagaaat tacgtgatta ttatgaagat atgaacggat ttattagtaa tgcgaagtta
     2041 gttgttgatg ataaaaaaat tcctaaacga atgccacaac aagattataa acagaaaaga
     2101 tggtttgggt tataaacagc aaatgagggg ttttatggca catttatctg tgaaattgcg
     2161 gcttttaata ctagcattaa tcgattcact gatagtgaca ttttcagtat tcgtaagtta
     2221 ttacatttta gaaccgtatt tcaaaacata ttctgtcaaa ttattaatat tggcagctat
     2281 atcactattc atatcgcatc atatttcagc atttattttt aatatgtatc atcgagcgtg
     2341 ggaatatgcc agtgtgagtg aattgatttt aattgttaaa gctgtgacga catctatcgt
     2401 tattacgatg gtggtcgtga caattgttac aggcaataga ccgtttttta gattgtattt
     2461 aattacttgg atgatgcact tgattttaat aggtggctca aggttatttt ggcgtattta
     2521 tcggaaatac cttggaggta agtcatttaa taagaagcca actttagttg ttggtgctgg
     2581 tcaagcaggt tcaatgctga ttagacaaat gttgaaaagt gacgaaatga aacttgaacc
     2641 ggtattagca gtcgatgatg acgaacataa acgcaatatc acaattactg agggtgtaaa
     2701 agtccaaggt aaaattgcgg atattccaga actagtgagg aaatataaga ttaaaaaaat
     2761 catcattgca attccaacta ttggtcaaga gcgtttgaaa gaaattaata atatttgcca
     2821 tatggatggc gttgagttat tgaaaatgcc aaatatagaa gacgtcatgt ctggtgagtt
     2881 agaagtgaac caacttaaaa aagttgaagt agaagattta ctaggcagag atcctgttga
     2941 attagatatg gatatgatat caaatgaatt gacgaataaa actattttag ttacgggtgc
     3001 aggtggttca ataggatcag aaatttgtag acaagtttgt aatttctatc cagaacgtat
     3061 tattctactt ggccatggtg aaaacagtat ttatttaatc aatcgtgaat tgcgaaatcg
     3121 cttcggaaaa aatgttgata tcgttcctat tatagcggat gtgcaaaata gagcgcgtat
     3181 gtttgaaatt atggaaacgt ataaaccata cgcagtttat catgcagcag cacacaagca
     3241 cgtgccgtta atggaagaga accctgaaga agcagtacgt aataatattt taggtacgaa
     3301 aaatactgct gaagctgcta aaaatgcaga ggtgaagaaa ttcgttatga tttctacgga
     3361 taaagccgtt aatccgccta atgtcatggg agcttcaaag cgaattgcag aaatgattat
     3421 tcaaagttta aatgatgaaa cgcatcgaac aaattttgtt gcagtgagat ttggtaatgt
     3481 acttggatcg agaggatctg tgattccact tttcaaaagt caaattgaag aaggtgggcc
     3541 agttactgtg acacatcctg aaatgacacg ttactttatg acaattcctg aagcttctag
     3601 actagttttg caggcagggg cattagcaga aggtggcgaa gtatttgtgc tagatatggg
     3661 agaaccagtg aaaattgtag atttggcacg taatttaatt aagctaagtg gtaaaaaaga
     3721 agacgacata cgcattactt atacagggat tagacccggc gaaaaaatgt ttgaagagct
     3781 tatgaataaa gatgaggttc atcctgaaca agtatttgaa aaaatttatc gtggcaaagt
     3841 acaacatatg aaatgtaatg aagttgaagc gattattcaa gacatcgtca atgactttag
     3901 taaagaaaaa attattaact atgccaatgg caaaaaggga gataattatg ttcgatgaca
     3961 aaattttatt aattactggg ggcacaggat cattcggtaa tgctgttatg aaacggtttt
     4021 tagattctaa tattaaagaa attcgtattt tttcacgcga tgagaaaaaa caagatgaca
     4081 ttcgaaaaaa atataataat tcaaaattaa agttctacat tggtgatgtg cgtgatagtc
     4141 aaagtgtaga aacagcaatg cgagatgttg attatgtatt ccatgcagca gctttaaaac
     4201 aagtgccgtc atgtgaattc tttccagttg aggcagtgaa gacaaatatt attggtacag
     4261 aaaatgtctt acaaagtgct attcatcaaa atgttaaaaa agtcatatgt ttatctacag
     4321 ataaggcagc gtatcctatt aatgctatgg gtatttcaaa agcaatgatg gaaaaagtat
     4381 tcgtagccaa atcaagaaat attcgtagtg aacaaacgct gatttgtggt acaagatacg
     4441 gtaatgtgat ggcttcaaga ggatcagtaa ttcctttgtt tatcgacaaa atcaaagctg
     4501 gagaaccttt aacgattaca gatcctgata tgacaagatt tttaatgagt ttagaagatg
     4561 ccgtagaact agttgttcat gcatttaagc atgcagaaac aggcgatatt atggttcaaa
     4621 aagcaccaag ctcaacggta ggggatcttg cgaccgcatt attagaattg tttgaagctg
     4681 ataatgcaat tgaaatcatt ggtacacgac atggagagaa aaaagcagaa acattgttga
     4741 cgagagaaga atacgcacaa tgtgaagata tgggtgatta ttttagagtg ccggcagact
     4801 ccagagattt aaattatagt aattatgttg aaaccggtaa cgaaaagatt acgcaatctt
     4861 atgaatataa ctccgataat acacatattt taacggtgga agagataaaa gaaaaacttt
     4921 taacactaga atatgttaga aacgaattga atgattataa agcttcaatg agataggaga
     4981 gattgacgtt gaatattgta attacaggag caaaaggttt tgtaggaaaa aacttgaaag
     5041 cagatttaac atcaacgaca gatcatcata ttttcgaagt acatcgacaa actaaagagg
     5101 aagaattaga gtcagcattg ttgaaagcag actttgtcgt gcatttagcg ggtgttaatc
     5161 gacctgaaca tgacaaagaa ttcagcttag gaaacgtgag ttatttagat catgtacttg
     5221 atatattaac tagaaatacg aaaaagccag cgatattatt atcgtcttca atacaagcaa
     5281 cacaagataa tccttatggt gagagtaagt tgcaagggga acagctatta agagagtatg
     5341 ccgaagagta tggcaatacg gtttatattt atcgctggcc aaatttattc ggcaagtggt
     5401 gtaagccgaa ttataactca gtgatagcaa cattttgtta caaaattgca cgtaacgaag
     5461 agattcaagt taatgatcgg aatgttgaac taacgctaaa ctacgtggat gatatcgtcg
     5521 ctgaaataaa gcgtgctatt gaaggaactc caacgattga aaatggtgta cctacagtac
     5581 caaacgtatt taaagtgaca ttgggagaaa ttgtagattt attatacaag ttcaaacagt
     5641 cacgtctcga tcgaacattg ccgaaattag ataacttgtt tgaaaaagat ttgtatagta
     5701 cgtatttaag ctatctacct agtacagact ttagttatcc cttacttatg aatgtggatg
     5761 ataggggttc ttttacagaa tttataaaaa caccggatcg tggtcaagtt tctgtaaata
     5821 tttctaagcc aggtattact aaaggtaatc actggcatca tactaaaaac gaaaaatttc
     5881 tagtcgtatc aggtaaaggg gtaattcgtt ttagacatgt taatgatgat gaaatcattg
     5941 agtattacgt ttctggcgac aaattagaag ttgtagacat accagtagga tacacacata
     6001 atattgaaaa tttaggtgac acagatatgg taactattat gtgggtgaat gaaatgtttg
     6061 atccaaatca gccagatacg tatttcttgg aggtatagcg catggaaaaa ctaaaattaa
     6121 tgacaatagt tggtacaagg cctgaaatca ttcgtttatc atcaacgatt aaagcatgtg
     6181 atcaatattt taatcagata ttagtacaca ctggtcaaaa ttatgattat acattgaatc
     6241 aaattttctt tgatgatttg gaattgagac aaccggacca ctacttagag gcagttggaa
     6301 gtaaccttgg agaaacgatg gggaatatta ttgcgaagac atatgacgtt ttattacgcg
     6361 aacaaccaga tgcactttta attcttggtg atacaaatag ttgtttagca gcagtatctg
     6421 ctaaacgatt aaagattcct gtgttccaca tggaagcggg taatagatgc tttgatcaga
     6481 atgtacctga agaaatcaat cgtaaaattg ttgaccatgt cagtgatgtg aatctacctt
     6541 atacggaaca tagcagacgt tatttattag atgaaggctt caataaagcg aatatctttg
     6601 tgacaggatc accgatgaca gaagtgatag aagcgcatcg agataaaatt aatcacagtg
     6661 acgttttaaa taaactagga ttagaaccgc aacaatacat tttagtatct gcgcatagag
     6721 aagagaatat cgataatgaa aagaatttta aatcattaat gaatgcgata aatgatattg
     6781 ccaaaaagta taaaatgcct gtgatttatt caatgcatcc aagaagttgg aagaaaattg
     6841 aagaaagtaa atttgaattt gatccattag ttaaaaagtt aaagccattt ggtttctttg
     6901 attataatgc attgcaaaaa gatgcatttg ttgtgctatc agatagtgga acattgtcag
     6961 aagagtcgtc tattttgaag ttccctggtg tccttattcg aacttccaca gaaagaccgg
     7021 aagtactaga taaaggtacg gttattgtag gtggtattac ctataacaat ctaatccaat
     7081 ccgttgaact agcaagagag atgcaaaaca ataacgaacc gatgattgat gctattgatt
     7141 ataaagacac taacgtttcg acaaaggtag ttaaaattat tcaaagctat aaagatatta
     7201 tcaatcgaaa tacttggagg aaatgacgat gaggatagcg attcttggcg ctactaacat
     7261 taagcatatg tcattattat cacattattt aaaccacatt gatttgaata tcaatgaggt
     7321 ggacattata tacactgaca aatatgatat cgaagaacat atccaaggca tcaataatta
     7381 ctataaatat aaagtagata ttaaagaaga ttggacattt atcaaaaaag ctattgctta
     7441 ctatcgattt aggccatacg ctatgaaaat tcttaaagaa aatcgttatg attttgtcat
     7501 agtatgggga agttatacag gacact
//
LOCUS       DAMZKC010000121         7526 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.122, whole genome shotgun sequence.
ACCESSION   DAMZKC010000121 DAMZKC010000000
VERSION     DAMZKC010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7526
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.122"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(<1..271)
                     /locus_tag="POB55_001970"
     CDS             complement(<1..271)
                     /locus_tag="POB55_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500930.1"
                     /translation="MLDVHFIYPRKLQNACEYFNLPTQKEGFEVTMHALRPENIHKIY
                     HDMDKTEQKFIETLISNGNPVTIINKRDVNILKLTNHLGFVYQLNE"
     gene            complement(386..1909)
                     /gene="ahpF"
                     /locus_tag="POB55_001971"
     CDS             complement(386..1909)
                     /gene="ahpF"
                     /locus_tag="POB55_001971"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HDI6500931.1"
                     /translation="MLNADLKQQLKQLLELMEGNVEFVASLGSDEKSKELKELLTEIS
                     DMSPRLSLSEKSLKRTPSFSVNRPGEETGVTFAGIPLGHEFNSLVLAILQVSGRAPKE
                     KQSIIDQIKNLEGSFHFETFISLTCQKCPDVVQALNLMSVINPNITHSMIDGAVFREE
                     SENIMAVPAVFLNGEEFGNGRMTIQDILSKLGSTADASEFENKEPYDVLIVGGGPASG
                     SAAIYTARKGLRTGIVADRIGGQVNDTAGIENFITVKETTGSEFSSNLAAHIDQYDID
                     AMTGIRATDIEKTDEAIKVTLENGAVLESKTVIIATGAGWRKLNIPGEEQLINKGVAF
                     CPHCDGPLFENKDVAVIGGGNSGVEAAIDLAGIVNHVTLFEFASELKADNVLQDRLRS
                     LSNVDIKTNAKTTEVVGEDHVTGIRYEDMSTGEEHLLNLDGIFVQIGLLPNTSWLKDA
                     VELNERGEIVIDCNNNTNVPGIFAAGDVTDQKNKQIIISMGAGANAALNAFDYIIRN"
     gene            complement(1925..2494)
                     /gene="ahpC"
                     /locus_tag="POB55_001972"
     CDS             complement(1925..2494)
                     /gene="ahpC"
                     /locus_tag="POB55_001972"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HDI6500932.1"
                     /translation="MSLINKEILPFTAQAFDPKKDQFKEVTQEDLKGSWSVVCFYPAD
                     FSFVCPTELEDLQNQYEELQKLGVNVFSVSTDTHFVHKAWHDHSDAISKITYTMIGDP
                     SQTITRNFDVLDEATGLAQRGTFIIDPDGVVQASEINADGIGRDASTLAHKIKAAQYV
                     RKNPGEVCPAKWEEGAKTLQPGLDLVGKI"
     gene            2986..3741
                     /locus_tag="POB55_001973"
     CDS             2986..3741
                     /locus_tag="POB55_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HDI6500933.1"
                     /translation="MSEHVYNLVKKHHSVRKFKNKPLSEDVVKKLVEAGQSASTSSFL
                     QAYSIIGIDDEKIKENLREVSGQPYVVENGYLFVFVIDYYRHHLVDQHAETDMENAYG
                     STEGLLVGAIDAALVAENIAVTAEDMGYGIVFLGSLRNDVERVREILDLPDYVFPVFG
                     MAVGEPADDENGAAKPRLPFDHVFHHNKYHADKETQYAQMADYDQTISEYYNQRTNGN
                     RKETWSQQIEMFLGNKARLDMLEQLQKSGLIQR"
     gene            complement(3822..5210)
                     /locus_tag="POB55_001974"
     CDS             complement(3822..5210)
                     /locus_tag="POB55_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HDI6500934.1"
                     /translation="MNAFLTLINIIVLVIFIVILHMMARKHISFAKRVFTALGIGIVF
                     GVLLHLVYGTHSNVITSTSDWFNIVGQGYVALLQMIVMPLIFISIVAAFTKIQIGEKF
                     AKIGSLIFIFLIGTVTIAAIVGVVYALVFGLDASTINLGNAEQARGSEIAKQAKDLTA
                     HTLPQQILELLPKNPFLDFTGQRATSTIAVVIFASFIGFAYLRVARKQPDHGELLKRA
                     IDAIYSLVMAIVTFVLRLTPYGVLAIMANTLSTSDFGAIWTLGKFLIASYAALITMYI
                     IHLIILSLLGISPIRYVKKTLEVLIFAFTSRSSAGALPLNVQTQTRRLGVPEGIANFA
                     ATFGLSIGQNGCAGIYPAMLAIMVAPVANVEIDLQFIVTLIAVVIISSFGVAGVGGGA
                     TFASILVLSTLNLPVALAGVLISVEPLIDMGRTALNVNDSMLAGTGTAKLTKHWDKDT
                     FDSNDNAALTSH"
     gene            5295..5393
                     /locus_tag="POB55_001975"
     CDS             5295..5393
                     /locus_tag="POB55_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500935.1"
                     /translation="MQLNVNMMKFDRWHKGICKQIVLNLVEKGEKG"
     gene            complement(6384..7340)
                     /locus_tag="POB55_001976"
     CDS             complement(6384..7340)
                     /locus_tag="POB55_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500936.1"
                     /translation="MLTKEFAQRVELSEKQVRKIVQHLEERGYQLSKTEYRGREATDF
                     KEEDIELFKDIADKVKQTNSYDLAFDELEKEKDFLQVIVKNDDKNLPTNQNVAQLVED
                     LRLEIQKMREERHLLGQMMNQVHQQQQELKELQNQLTSKIDSNSESLKAIQTSQEAIQ
                     EAQASQAKVLAESTNKVEKNAVTEDKADSKDSKVAGVNTSTDAKTDTKADNAGDGTTT
                     KVDKEDQISATEAIEKASVEQSKNGNAAETSNKEATIDAEAQHDAEQQVAEAHAEASK
                     QATSNDSLEAKAENDSTASQSEMSEPKPQEEKKGFFARLFNL"
BASE COUNT         2376 a         1294 c         1390 g         2466 t
ORIGIN      
        1 cttcgttaag ttgataaaca aaacctagat gatttgttag ctttaaaata ttaacatcgc
       61 gtttatttat aattgtgaca ggattaccat tagatataag tgtttctata aatttttgtt
      121 ctgtcttatc catatcatga taaattttat gtatattttc agggcgtaaa gcatgcatcg
      181 tgacttcaaa tccttctttt tgggtcggta aattaaaata ttcgcatgcg ttttgtaatt
      241 ttcttgggta tataaaatga acatcaagca tttttgcaca cctcgttgta tatattaagt
      301 ttatcataac atgattttat gtcgggataa aaaaataaca gcatcttaac aaatgtaaga
      361 tactgtcagt gaaatgaatg aaactttagt ttctgataat atagtcaaag gcatttaatg
      421 ctgcatttgc accagcgccc attgaaataa taatttgttt gttcttctga tctgtgacat
      481 cgccagcagc aaatattcca ggtacgttag tattattgtt acaatcaata acaatttcac
      541 cacgttcgtt taattcaaca gcatctttta accatgatgt gtttggaagt aaaccaattt
      601 gaacaaagat accatctaag ttaagtagat gttcttcgcc ggtgctcatg tcttcgtaac
      661 gtatacctgt aacatggtct tctccgacaa cttcagtagt tttggcattt gttttgatat
      721 caacatttga taaagaacgt aaacgatctt gtaacacgtt gtctgctttt aattcgctag
      781 cgaattcgaa taatgtaaca tgattaacga taccagcaag gtcaattgct gcttcaaccc
      841 cagagttacc gccaccgata actgctacgt ctttattttc aaatagaggt ccgtcacagt
      901 gagggcagaa tgcaacacct ttattaatca attgctcttc acctggaatg tttagcttac
      961 gccaacctgc accagtagca ataatgactg ttttactttc taagacagca ccgttttcta
     1021 acgtaacttt aattgcttcg tcagtctttt cgatatctgt agcacgtata cctgtcattg
     1081 catcaatgtc atattgatca atgtgcgctg ctaagttaga agaaaattca gaaccagttg
     1141 tttctttaac agtaatgaag ttctcaatac cagcagtatc attaacttgg ccaccgatac
     1201 gatcagcaac tataccagta cgtaaacctt tacgtgctgt gtaaatcgct gcactaccac
     1261 tagcaggacc accaccaacg attaagacat cataaggttc tttattttca aactcagatg
     1321 catctgccgt actgcctagt ttcgaaagaa tatcttggat tgtcatacga ccattgccaa
     1381 attcttcgcc atttaaaaag acagcaggga ctgccatgat gttttcagat tcttcacgga
     1441 acactgcacc atcaatcata gaatgcgtga tgttagggtt gatcacactc attaagttaa
     1501 gtgcttgaac gacatcagga catttttgac acgttaaact aatgaatgtt tcaaaatgga
     1561 atgaaccttc taagttttta atttggtcaa tgattgactg tttttcttta ggtgcacgac
     1621 cactaacctg taaaattgct aaaacaagtg agttaaactc gtgacctaat ggaatacctg
     1681 caaatgttac acctgtttct tcgccaggac gattgactga gaaacttggt gtacgcttta
     1741 aagacttttc agaaagtgaa agtctaggtg acatatcaga aatttctgtt aataattctt
     1801 tgagttcctt agatttttca tctgaaccaa ggctggcaac gaattcaacg ttgccctcca
     1861 ttagttctaa taattgttta agttgttgtt ttaaatcagc attaagcatg gttgtaatgc
     1921 ctccttagat tttacctact aaatctaaac caggttgcaa tgttttagcg ccttcttccc
     1981 atttagctgg gcatacttcg ccagggtttt tacgaacata ttgagctgct ttgattttgt
     2041 gagctaatgt actagcgtca cggccaattc cgtcagcgtt aatttcagat gcttgtacaa
     2101 caccgtctgg gtcgataatg aatgtaccac gttgagctaa accagtagct tcatctaata
     2161 catcaaaatt acgagtgatt gtttgtgatg ggtcaccaat catagtgtaa gtgattttgc
     2221 taattgcatc tgaatggtca tgccatgctt tgtgtacgaa gtgagtatca gttgatactg
     2281 agaatacatt tacgcctaat ttttgtaatt cttcatattg gttttgtaag tcttctaatt
     2341 cagttggaca aacgaatgag aagtcagcag gatagaagca tactacgctc caagaacctt
     2401 ttaaatcttc ttgtgtaact tctttaaatt gatctttttt tggatcgaaa gcttgcgctg
     2461 taaatggtaa gatttctttg ttaattaatg acataaatat cttcctccta agaatttaag
     2521 tatgaattag aactatcaat tgattgtgct taattataat aattctaatc tcttagttag
     2581 cattattaca ttttgatcca gaatagtcaa ctggataact ttgtaaagtg aatgattact
     2641 tttaaaataa agaaagataa tataaagtgc tttgataatg gattttgtag ttgatgattt
     2701 aaaaggttgt gtctatattt aatatcttga ttttaatgta aaaaatgtaa aaaaagaaga
     2761 tttgtattct caactaagtc aaccttattg ataatggtgt gagaatattt gttcgagatg
     2821 gatgaaggta atgagtgaga aactggattt ttaaagtatg agacaatatt tttaaaagtt
     2881 caattattaa cttataagca aataattgct ataaaaaagt ttggacgtgt acaattgaaa
     2941 tatgaagatt ttaaattaat tgtaaagtat cgaggagtgg gtaacgtgtc agaacatgta
     3001 tataatcttg tgaaaaagca tcattctgtt agaaaattta agaataaacc tttaagtgaa
     3061 gacgttgtta agaaattggt agaagctgga caaagcgctt cgacgtcaag tttcctgcaa
     3121 gcatactcaa ttattggtat cgacgatgag aagattaaag aaaatttacg agaagtttct
     3181 ggacaacctt atgttgtaga aaatggctat ttattcgtct ttgttattga ttattatcgt
     3241 catcatttag ttgatcaaca tgctgaaact gatatggaaa atgcatatgg ttcaacggaa
     3301 ggtttgctag taggtgcaat cgatgcagca ttagttgccg aaaatattgc ggtaactgct
     3361 gaagatatgg ggtatggcat tgtcttttta ggatcattaa gaaatgatgt tgaacgagtt
     3421 cgagaaattt tagacttacc tgactatgtc ttcccggtat ttggtatggc agtaggggaa
     3481 cccgcagatg acgaaaatgg tgcagccaag ccacgcttac catttgacca tgtcttccat
     3541 cataataagt atcatgctga taaggaaaca cagtatgcac aaatggcaga ttacgaccag
     3601 acaatcagcg agtactataa tcaacgtaca aacgggaatc gcaaagaaac atggtcgcag
     3661 caaattgaga tgttcctagg aaacaaagca agattagata tgttagaaca attgcaaaaa
     3721 tcaggcttaa tacagcgata gcaagatacc aaaataaccc gcccccctct agcttaaaat
     3781 gataagtata gctagagggg gcgggtattt cttgcaatga attagtgtga agttaatgca
     3841 gcattatcat ttgaatcgaa agtatcttta tcccaatgtt tagttaactt ggcggtacct
     3901 gtaccagcta gcattgaatc gttcacgttt aatgctgttc tacccatgtc aatcaatggt
     3961 tcaacggaga tgagcacgcc ggctaaagcg actggcaagt ttaacgttga caacaccaat
     4021 atggatgcaa atgtagcccc gccaccgacg ccagcaacgc cgaatgaact aataatcacg
     4081 acagcgatta acgttacaat aaattgtaaa tcaatttcta cattagcgac gggtgcgacc
     4141 ataattgcaa gcatggcagg gtaaatgcct gcacaaccat tttgtccaat cgacaatcca
     4201 aatgtcgcag cgaaattggc aataccttct ggcacgccta gacgtcttgt ttgtgtttgt
     4261 acattcaatg gtaaggcacc cgcgcttgag cgtgatgtga atgcaaagat taatacttcc
     4321 aaagtctttt taacatagcg aattgggcta atacctaaca ggcttaaaat aattaagtga
     4381 atgatataca tcgtaattaa tgcagcgtac gatgcgatta agaattttcc taaagtccaa
     4441 atggcgccaa agtcacttgt cgataatgtg ttggccataa ttgctaatac accgtatggc
     4501 gttaaacgta agacgaacgt cacaatcgcc attactagtg aatagatagc gtcaatcgca
     4561 cgcttaagca attcaccatg atcaggttgt ttgcgtgcta cgcgtaaata agcaaatcct
     4621 ataaacgaag caaatatcac gacagcaatc gtggaagttg cacgttgtcc agtgaaatct
     4681 aagaatggat ttttaggcaa taattccaaa atttgttgtg gtaacgtatg tgctgttaaa
     4741 tctttcgctt gtttagcaat ttcgcttcca cgcgcttgtt cagcgttacc aaggttaatt
     4801 gttgatgcat ctaaaccaaa caccaaggca taaacaacac caacaattgc agcaatggtg
     4861 acagtgccaa ttaaaaagat aaaaatgaga ctaccaattt tagcaaactt ttctccgatt
     4921 tgaattttag tgaatgcagc gacaatagaa atgaaaatta aaggcatcac aatcatttgc
     4981 aacaatgcaa cgtaaccttg tccgacaatg ttgaaccagt cacttgttga tgtaataaca
     5041 ttcgaatgtg ttccatagac aagatgtaat aacacgccaa atacaatgcc gatacctaaa
     5101 gctgtaaaca cacgtttcgc aaaagatata tgtttgcgag ccatcatgtg caatattacg
     5161 atgaaaatca ccaatacaat aatattaatc agtgtaagaa aagcattcat gaacgtcact
     5221 cctcaaattt ttgaatataa ttccgactag tatgctaaga ataatattaa ttttaaaata
     5281 tttcaagagg gcatttgcaa ttaaatgtaa acatgatgaa attcgaccgt tggcataagg
     5341 gaatttgtaa acaaatcgtt ttgaatttag tagagaaagg tgaaaagggt taatagtgtt
     5401 atgacctagt cgattaaagt tgatatttga aaatgaagca gggaattaaa cgcaataaat
     5461 taaataagtt gtcattacag cgttaataat agaaatgatt ttaacgggaa aaaagtgatg
     5521 aatatttgga aaagatatat atcgtgtact gtcatgagag ataagatatg agaaaacaaa
     5581 tgtatccttg aatcgagatg tggctatggc ggtttaataa aaattgctaa cctcatattt
     5641 attgaataaa aagataataa atgatcaacc ttgaaaagac acttcgaaca aacacgttgt
     5701 aaaaaattct gtcgaatcaa aattagcatc ttttgagggt tcgtgtgtgc aggtggtagt
     5761 tcaaaagcta ccgtaaaaca aatatttttg tttgagcaaa aaggcgtttg tagaaaatgt
     5821 gaatatgagt gtgcccttgt gcgattcgtc agttactaac acagccaaac atttaaattg
     5881 aatgggctta taagatgaat taatttagga atacacagaa gccatcccat ttaaaagtaa
     5941 catactacaa aaatttattc aaaattcaat attaatcata atgaataatt aaaaaacctg
     6001 aaacaaatta gtttgatatg ctctcttcaa agtagatatt gaaaggagta tatcagcttg
     6061 tttcaggttt tatgaattga gtattaaatt tgaaagtaaa ttacagttct atagttttat
     6121 aaaagatcag tttttaaatc aaagctatat attattgagt attacaggct gggacataaa
     6181 tcaatgttct atgctctacg aagttatatt ggcagtagtt gactgaacga aaatgcgctt
     6241 gtaacaagct tttttcaatt ctagtcaacc ttgccggcgg ggccccaaca aagagaaatt
     6301 ggattcccga tttctacaga caatgcaagt tggggtggga cgacgaaata aattttgcga
     6361 aaatatcatt tctgtcccac tccctacaag ttgaataaac gtgcgaagaa accttttttc
     6421 tcttcttgag gttttggttc tgacatttca gactgtgaag ctgtgctgtc attttctgct
     6481 ttcgcttcca atgaatcgtt cgaagtagct tgtttagaag cttctgcatg ggcttctgct
     6541 acttgttgtt cagcatcatg ttgcgcttct gcatctattg tagcttcttt atttgaagtc
     6601 tcagctgcat tgccattttt agactgctca acgctggctt tttcaattgc ttcagttgca
     6661 gaaatttgat cctctttatc tactttagta gtagtgccat cgcctgcatt gtctgcttta
     6721 gtatccgtct tagcgtctgt tgacgtattc actccagcaa ccttcgaatc tttactgtca
     6781 gctttatctt cagtaacagc attcttttca actttatttg ttgattcagc caatacttta
     6841 gcttgagatg cttgtgcttc ttggatagcc tcttgtgatg tttggatagc ttttaaggat
     6901 tcgctatttg aatcgatttt agatgtaagt tgattttgaa gttcttttaa ttcttgttgt
     6961 tgctgatgta cttgattcat catttgacca agtaggtgac gttcttcgcg cattttctgg
     7021 atttctaggc gtaaatcttc tactaattga gcgacatttt gattagtagg taagtttttg
     7081 tcatcgtttt tgacaatgac ttgcaggaag tctttttctt tttctaattc atcaaacgct
     7141 agatcataac tatttgtttg ttttactttg tcggcaatgt ctttgaaaag ctcgatatct
     7201 tcttctttga aatcagttgc ttcacgacca cgatattcag tcttgcttag ttggtaacca
     7261 cgttcttcta aatgttgaac aattttacgg acttgcttct cacttagttc tacgcgttgt
     7321 gcaaattctt tagttaacat actttaaagt cctttctgca tatgctttat gtatactgac
     7381 gtttgtctac gggcagtgaa acgtcagtct tgcttcatac atagtttaaa cgttattgtt
     7441 agtgatttca agtttgttta aaatggttta cgtaaatcca tttcttctaa taaagcataa
     7501 ataatttcac gtgcgatacc atttgc
//
LOCUS       DAMZKC010000122         7333 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.123, whole genome shotgun sequence.
ACCESSION   DAMZKC010000122 DAMZKC010000000
VERSION     DAMZKC010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7333
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.123"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            157..453
                     /locus_tag="POB55_001977"
     CDS             157..453
                     /locus_tag="POB55_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDI6500937.1"
                     /translation="MTYEQEDDFKLLKRRILGIGFVLPEGQVFTFHDLNRRANVQCSK
                     AVQQNVGRWFAYFVKNAPSVPFIIIGKNTNGHLVYMKVGPNPLQDSNPSKGGVR"
     gene            453..2222
                     /locus_tag="POB55_001978"
     CDS             453..2222
                     /locus_tag="POB55_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017176075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HDI6500938.1"
                     /translation="MSHYIIFEAGPYQLDAKAWRKTFPPKKDGDKEKVVPISSCIVVE
                     AKFWHASENTEKIIISNGKDKEHTFESDILTTRNLPSLIKYGHSINESYIRDLSYALQ
                     LMRDELPTSDLYDGSGIIETPHGYIVMVDTVYTSKQYDQLASLEPIVDSAYLLEPKGT
                     FKDWLNMYMNEVKGHLLLELAVIFGISALVTAFLKHKHEVEFAGIIFSFTGQSSTGKS
                     TAAALAVSVAGNPTKGNKTLFRSWNGTRNALEGYLSNNYGIPMVFDELSSATFKDTTG
                     LLYSIAEGQGRQRSNVHGEVKTPKNWGTSVISTSEYSIFTDSAQNDGLRVRTIEINEQ
                     FTTNATNADNIKKAVALNYGHVLPLVAQYLINREEEVIQWFKDEHHWFKAKLKSEANN
                     TGIRMFKRYAVITTSAQILGRVLATNIDIAKIRDYFIDYHTHTVSERSLADKAIEVIT
                     QFVAQNRGKFSDDTALKNMIENYGLIALKDDYIEVKIIATVFKDMLKEQHFQDVNNVV
                     NALRDKGFIESDRDRITTKRTVKDDYGKKQSLVFYHLKLDSEYASIFGLTKDAEPIKA
                     KINLDNKKMLESWKKQNDDLIDF"
     gene            2410..3759
                     /locus_tag="POB55_001979"
     CDS             2410..3759
                     /locus_tag="POB55_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002486190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDI6500939.1"
                     /translation="MKQAIGYLRQSTTKQQSLAAQKQTIKALAEKHNIQHITFYSDKQ
                     SGRTDKRNGYQQITELIQQGQCDVLCCYRLNRLHRNLKNALKLMKLCQTYHVHILSVH
                     DGYFDMDKAFDRLKLNIFISLAELESDNIGEQVKNGIKEKARQGKLITTHAPFGYHYH
                     NGTFTIDTVKSPTVKAVFNYYLQGYGYKKIAQYLEADDNFINRKPYQVRNIILNPNYC
                     GRVINQYGQYENMFPAIVSTTIYEEAQVTRTQKQVKCKPSENQLKQKIKCPYCHSTLT
                     NMTVRKPDHSLRYYVCPQNMNNARFVCEFKGINAQELETSVLATCQDFFQNQQLYSKI
                     NHTIQQRLKRQRDIEIKTTLNHEQLIEKLAQGKIDAETFKEQTHSLHQQSKPISSISA
                     YQIRKAFQNIIQQRFTLNMLYPYIDEINISKNKSLAGIYFKNEPLNIVKQTMKLSIV"
     gene            3781..5406
                     /locus_tag="POB55_001980"
     CDS             3781..5406
                     /locus_tag="POB55_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDI6500940.1"
                     /translation="MDKMKKKLVGGYIRVSTERQVEGYSIEGQITQIEQYCQFNGYEL
                     VDIYADRGISGKSMNRPELQRMLNDAKNGKLDCVMVYKTNRLARNTSDLLTIVEELHR
                     QNVEFFSLSERMEVKNSTGKLMLQILASFSEFERNTILENIYNGQHQRALKGYYQGNL
                     PLGYNNIPDNKKELMINQHEANIVKYIFESYAKGHGYRKIANALNHKGYVTKKGNPFS
                     INSITYILVNPFYIGKIQFAKYKDWNEKRRKGLNDKPIIADGKHPPIISQELWDKVQS
                     RKKQVSQKPQVHGKGTNLLTGIIHCPQCGAPMAASNTTNTLKDGTKKRIRYYSCSNFR
                     NKGSKVCSANSVRADVIEKYVMDQILEIVKSDKVLKQVVERVNQDNQVDVAALNHDIA
                     YKQQQFDEVNIKLKNLVQTIEDNPDLTSALKPTIHQYETKLNDITNQMNQLKQQQNQE
                     KPSYDTKQIAALLQRIFQNIESMDKSQLKALYLTVIDRIDIRKDENHKKQFYVTLKLN
                     NEIIKQLFNNNNLDEVLLSTSSLFLPQTLYFQI"
     gene            5872..6213
                     /locus_tag="POB55_001981"
     CDS             5872..6213
                     /locus_tag="POB55_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500941.1"
                     /translation="MKTTTQELKQYITRLFQLSNNETWECEALEEAAENILPTRFVDH
                     TPLAHLTLETYTYYNDELHELSIYPFLMYANNQLISIGYLDHFDMDFLYLTDTKNTII
                     DERHLLQKGGE"
     gene            6306..6617
                     /locus_tag="POB55_001982"
     CDS             6306..6617
                     /locus_tag="POB55_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500942.1"
                     /translation="MKINRYITKGISKQLSLDLQILLWNMVKDRDNQLNTDYLHIFKL
                     QEDENILSITHEQEQPAYKLEYHYTNYIKNQNALPKKVYVIREDDVDVFYYVMLLPEE
                     Y"
     gene            6633..7136
                     /locus_tag="POB55_001983"
     CDS             6633..7136
                     /locus_tag="POB55_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012086592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HDI6500943.1"
                     /translation="MNTIKSTIHTEAIFSDDKQHRYLLKKTWDEKKPACTVITMYPHL
                     DGVLSLDLTTVLILNQLANSEQYGAVYLVNLFSNIKTPENLKHIKEPYDKHTDIHLMK
                     TISESDTVILAYGAYAKRPVVIERVSQVMEMLKPHKKKVKKLINPATNEIMHPLNPKA
                     RQKWTLK"
     gene            7150..>7333
                     /locus_tag="POB55_001984"
     CDS             7150..>7333
                     /locus_tag="POB55_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500944.1"
                     /translation="MNHETTQSDWRTVPNCLASQNYISIVKGLVHHFTAIEDEEILDK
                     IYDDFMDDDSITTVLNN"
BASE COUNT         2764 a         1304 c         1210 g         2055 t
ORIGIN      
        1 caacgatttt tttatcgttg ggttttttaa ttaacaaaag gtgtaatacc aatgtcctaa
       61 tattttcctc tcaaatttag aactttatat ctcagtgtag atataagata caaaggcatc
      121 atttaaatga tgactaatat acaaaggatg atccaaatga cttacgagca agaagatgat
      181 tttaaactgt tgaaaagaag aattttagga attggattcg ttttaccgga gggacaagta
      241 tttactttcc atgacttgaa ccgtcgtgca aatgtacaat gctcaaaagc tgtacaacaa
      301 aatgttggac gttggttcgc atattttgtt aaaaatgcgc caagtgttcc atttattatt
      361 atcggtaaaa ataccaatgg tcatttagta tatatgaaag tcggacctaa tccactccaa
      421 gatagcaacc cttcgaaagg aggtgttcgc taatgagtca ttacatcatt tttgaagcag
      481 gtccatacca attagatgca aaggcgtggc gtaaaacttt tccacctaaa aaagatggag
      541 ataaggaaaa agtagtgcct atatctagtt gtatcgttgt tgaagctaaa ttttggcatg
      601 cctcagaaaa tacagaaaaa ataattattt ctaacggtaa ggataaagaa catacatttg
      661 aatctgatat tttaacaact cgaaacttac cttccttgat taaatatggt cacagtatca
      721 atgaaagtta tataagagat ttgagctatg cactacagtt aatgcgcgat gaactgccca
      781 cttctgattt atatgatggc tcgggcatca tagaaacacc ccacggttac attgtaatgg
      841 tagataccgt ttacacatct aaacaatatg atcagttagc atctttagag cctattgtcg
      901 atagtgctta tctattagag cctaaaggga catttaaaga ttggttaaat atgtatatga
      961 acgaagtcaa aggtcatctt cttctagagt tagcggtcat atttggaatt tctgccttag
     1021 tgacagcctt tctgaagcat aaacatgagg ttgaatttgc tggtatcatc tttagtttta
     1081 caggccagtc atcaaccggt aaatcaacag cggcagcttt agcggtatcc gtcgctggaa
     1141 atccaaccaa aggtaataaa acgctttttc gaagttggaa tggcacacgt aacgctcttg
     1201 aaggttactt gagtaacaat tatggcatac ctatggtatt tgacgagctc tcctcagcaa
     1261 cttttaaaga tacaacaggg ttattgtact ccatcgctga aggtcaaggg cgtcaacgtt
     1321 caaacgttca tggagaagtt aaaacaccta agaattgggg aacttctgtc atttcaacgt
     1381 cagaatacag tatttttact gattcagctc aaaacgatgg gttacgtgta agaaccattg
     1441 aaattaatga gcaatttact acaaatgcga ccaatgctga caatattaaa aaagctgtgg
     1501 cgctaaacta tggtcatgtt ttaccgttag tagctcaata ccttataaat cgtgaagagg
     1561 aagttattca atggtttaaa gatgaacatc attggtttaa agctaaattg aagagtgaag
     1621 caaacaatac aggtattcgt atgtttaagc gttatgcagt cattacaaca tctgcgcaaa
     1681 tattaggtcg tgttttagct acaaatattg atatcgctaa aataagagac tactttattg
     1741 attatcatac tcatacagtc tctgaacgat cactggcaga taaagcgatt gaagtcatta
     1801 cacaatttgt agcacaaaac cgtggtaagt tttctgatga cacagcgtta aagaatatga
     1861 ttgagaatta tggactaatt gccttaaaag atgattatat cgaggtcaaa atcattgcga
     1921 ctgtatttaa agatatgctg aaagaacaac actttcagga tgtcaacaat gtcgtgaatg
     1981 cactaagaga taaaggattt atcgaatctg accgtgatcg aatcacgaca aaaagaacag
     2041 taaaagatga ctatggcaag aaacagtcac ttgtttttta ccatttaaag ttagattcag
     2101 aatatgcgtc tatttttggt ctaactaaag atgctgaacc tattaaagct aaaattaact
     2161 tggataacaa aaagatgctt gaatcatgga aaaaacagaa tgatgattta attgattttt
     2221 aataacttaa agatgcaatc gtacaggatt agataaaatg gatgaattga ttgaattact
     2281 agctgattta gttgttgaag aaattaatca tgctgatgac taataataac gcccaacacc
     2341 agcaggtgtt gggctcatct ataaattcgt tcataaatat atattatcac tttggaagga
     2401 ggaaatacta tgaaacaagc aataggttac ttacgacaga gcactacaaa gcaacaatcc
     2461 ttagcagcac aaaaacaaac catcaaggca ttagccgaaa aacataatat tcaacacatt
     2521 accttttata gcgataaaca atcaggacgt actgataagc ggaatggtta tcaacaaatt
     2581 actgaactga ttcaacaagg acaatgtgac gtattatgtt gctatcgtct aaatcgactt
     2641 catcgtaatc taaaaaatgc attaaagctc atgaaattat gtcaaacgta tcatgttcat
     2701 atcttaagcg ttcatgatgg ctacttcgac atggataaag cattcgatcg gctcaaactc
     2761 aatattttca tcagcttggc cgaactagaa tctgataata taggcgaaca agtcaaaaat
     2821 ggaatcaaag aaaaagcgag acaaggtaaa ctaattacaa cacatgcgcc ctttgggtat
     2881 cactatcata atggtacttt cacgatagac acagtaaagt caccaacagt aaaagctgtg
     2941 ttcaattatt accttcaagg ttatggttat aaaaaaattg cgcaatactt agaagctgat
     3001 gataacttca ttaatcgcaa gccttatcaa gtgcgtaata ttatcctcaa tcctaattac
     3061 tgtggccgtg ttatcaatca atacggacaa tatgaaaaca tgttcccagc tattgtcagt
     3121 acaacgatat acgaagaagc tcaagttacc cgaactcaga aacaagtaaa atgtaagccg
     3181 tcagaaaatc aactcaaaca aaaaatcaaa tgtccctatt gtcattcgac actcacaaac
     3241 atgactgtcc gaaaaccaga tcattcattg cgttactatg tctgtccgca aaatatgaat
     3301 aacgctcgct ttgtttgtga atttaaagga atcaacgcac aagaattaga aacaagtgtt
     3361 ttagcgactt gtcaggactt ctttcaaaat caacagctct attcaaaaat aaaccatact
     3421 attcaacaac gactcaaaag acaaagagat atagaaatta aaactacact caatcacgag
     3481 cagctgatag aaaaattagc ccaaggcaaa attgatgcag aaacgttcaa agaacaaacg
     3541 cactcattac atcaacaatc aaaacctata tcatcaatca gtgcgtatca aattcgaaaa
     3601 gctttccaaa acatcattca acaacgtttc acgctaaaca tgttgtaccc ctatattgat
     3661 gaaattaata tttctaaaaa taaaagcctc gctggcatct atttcaaaaa cgaaccactg
     3721 aatattgtaa agcagactat gaaattatca attgtataat taaagaaagg atggattata
     3781 atggataaaa tgaagaaaaa gcttgtagga ggctacattc gcgtgtccac agagagacaa
     3841 gtagagggtt atagcatcga gggacaaatt acacaaatag agcaatattg ccaattcaac
     3901 ggctatgaac tcgttgatat atatgcagat cggggtatat caggaaaatc tatgaaccgt
     3961 cctgaattac agcgcatgtt aaatgatgct aaaaatggaa aattagactg tgttatggtt
     4021 tataaaacaa atcgtttggc acgtaatact tccgatttac ttacaatcgt cgaagaactt
     4081 catcgtcaaa atgttgaatt ttttagcttg tctgaacgta tggaagtcaa aaattcaaca
     4141 ggcaagttaa tgctccagat acttgcaagt ttttccgaat ttgaaagaaa tacaatttta
     4201 gagaatattt acaacgggca acatcaaaga gctttaaagg gctattatca aggcaatctt
     4261 ccattaggat ataataacat acctgataat aaaaaagaat taatgattaa tcaacatgaa
     4321 gctaacatcg ttaaatatat ctttgaatct tatgccaaag gtcatggtta tcgtaaaata
     4381 gccaatgcac tcaatcacaa aggctatgtg actaaaaaag gcaatccatt tagtatcaat
     4441 tcaataacct atatcttagt caatccattc tacattggta aaattcaatt tgcgaagtac
     4501 aaagattgga atgaaaagcg tcgtaaagga ctaaatgata aacctattat agctgatggt
     4561 aaacaccctc ctattattag tcaagaatta tgggataaag tccaatcacg caaaaaacag
     4621 gtcagtcaaa aacctcaagt tcatgggaaa ggaaccaatc tattaacagg tattattcac
     4681 tgcccacaat gtggtgcacc aatggcagct agtaacacaa cgaacacatt gaaagatggt
     4741 actaagaagc gaatacgtta ttattcttgt agtaacttcc gaaacaaagg ctcaaaagta
     4801 tgttctgcga atagcgttag agccgatgtc attgagaaat acgttatgga tcaaatactt
     4861 gaaattgtca aaagcgataa agttctcaaa caagttgtcg aacgtgttaa tcaagataat
     4921 caagtcgatg tagctgcact caatcatgat attgcttata aacaacaaca atttgatgaa
     4981 gtaaacatca aacttaaaaa tctcgttcaa accatcgaag acaatccaga cttaacatct
     5041 gcactcaaac caaccattca tcaatatgaa acaaaactga atgatattac aaaccaaatg
     5101 aatcaactca agcagcaaca aaatcaagag aaaccatctt atgatacgaa acaaatcgct
     5161 gccctattac aacgaatatt tcaaaatata gaatccatgg ataaatcaca gctcaaagca
     5221 ttatacctaa cggttattga ccgtattgat attcgtaaag acgagaatca taaaaaacaa
     5281 ttctatgtca ctctcaagct caataatgaa attattaaac aactttttaa taataacaat
     5341 ctagacgaag tgctcctcag cacttcgtct ttgtttttgc ctcaaacact ctactttcaa
     5401 atataatatc tatgtactgt cctttcgtca ttcaataaca ctcatctaac aaatatattc
     5461 aacacgctaa caaatcatgg ctcaatgtga agcttatatt gatatgacga aagaacatcc
     5521 caaaacaaaa tataagtggg atagtaacgg gattggctaa cttcatgtag gagaaggatt
     5581 acgcaagcgt aatcccaaaa tcccgttatt atttataaat aaataatata aaaagaaaaa
     5641 gtaagtcgta ataacaccac caaatccaaa taatatataa atatatacct atatttccca
     5701 acaaatgagc gggataacgg gattttcttg tcatatcaat gtttgtagca gtttttcata
     5761 tgggattttc tcttattccc accgggattc gtaataaatg ataaatcaaa tatatattat
     5821 ctcagtgatg tatatgaaat acatcgctaa cactatctag gaggcaatga tatgaaaaca
     5881 accactcaag aactcaaaca atatataact cgtctattcc aactatctaa caatgaaaca
     5941 tgggaatgtg aagcgttaga agaagcagca gaaaatatac ttcctactcg ctttgtagac
     6001 cataccccac ttgcgcatct tacacttgaa acttatacct actataacga tgaactacat
     6061 gagctaagta tctatccatt tcttatgtac gccaataacc aactcatcag tattggttat
     6121 ctggatcatt ttgatatgga ctttctatac ctcacagata ctaaaaatac aattatcgat
     6181 gaacgtcatc tattacaaaa aggaggagaa taatatgaac tggatcaaga tcgctcaact
     6241 atctgttaca gttattaacg aagtaattga catcatgaaa gaaaagcaaa atggaggaaa
     6301 atagtatgaa aatcaatcga tacatcacaa aaggcattag taaacaacta tctctagacc
     6361 ttcaaatcct actttggaat atggtaaaag atcgagacaa tcaacttaat acagattacc
     6421 tacacatttt taaactacaa gaagatgaaa atatactctc aatcacacat gaacaagaac
     6481 aacccgcata caaattggaa tatcactata caaactatat aaaaaatcaa aatgcattac
     6541 ctaagaaagt ctacgtcatc cgagaagatg atgtagacgt tttttattat gtgatgcttt
     6601 taccagaaga atactaaagg aaatgatgat atatgaatac aattaaaagt acaatacaca
     6661 cagaagccat atttagcgat gacaaacagc accgctattt actcaagaaa acttgggatg
     6721 aaaagaaacc agcttgtaca gtgataacga tgtaccccca tttagatggt gtgttatcac
     6781 tcgatcttac tactgttctt attcttaacc aattagcgaa ttctgaacaa tatggtgctg
     6841 tatatcttgt gaatctattt tcaaatatta aaaccccaga gaaccttaaa catatcaaag
     6901 agccttatga caaacacaca gacattcact tgatgaagac gattagtgaa agtgacacag
     6961 tgattctagc ttatggcgcc tatgctaagc gaccagttgt tatagaacgt gtatcacagg
     7021 tgatggaaat gttaaaacct cataaaaaga aagtcaaaaa gctcataaat ccagcaacaa
     7081 atgaaattat gcatccactc aatcctaaag cgcgtcaaaa atggacattg aaataaagga
     7141 ggcttaccta tgaaccatga aactacacaa tcagactggc gaacggttcc taattgctta
     7201 gcatctcaaa attatatatc tatcgtaaaa ggactagtac atcattttac agcgattgaa
     7261 gatgaagaaa tacttgataa aatctatgac gattttatgg atgatgactc tataacaacg
     7321 gtccttaata atg
//
LOCUS       DAMZKC010000123         7209 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.124, whole genome shotgun sequence.
ACCESSION   DAMZKC010000123 DAMZKC010000000
VERSION     DAMZKC010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7209
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.124"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            50..700
                     /locus_tag="POB55_001985"
     CDS             50..700
                     /locus_tag="POB55_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excalibur calcium-binding domain-containing
                     protein"
                     /protein_id="HDI6500945.1"
                     /translation="MKITYKYRGDLPLNTENNKNQNRSVKNSERRGMLKGCGGCLISF
                     ILLIILLSACSMMFSNNDNSTSNQSSKTQLTQKDENKNEDKPEEKSETATDEDLQSTE
                     EVPANENTENNQHEIDEITTKDQSDDEINTPNVAEDKSQDDLKDDLKEKQQSSNHHQS
                     TQPKTSPSTETNTQQSFANCKQLRQVYPNGVTADHPAYRPHLDRDKDKRACEPDKY"
     gene            781..1776
                     /locus_tag="POB55_001986"
     CDS             781..1776
                     /locus_tag="POB55_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HDI6500946.1"
                     /translation="MSNQFKSEEERRQWEQFQAFQNQQNQQNQQYGQKKSKKGWFWGC
                     GGCLVLFILIIIGISACTAGITGNLGGNSSKETNKTHKIGETVKNGDLEVTVNSVETM
                     KSVGPSIAPTNAKGTFVVADVTIKNKGKEALTIDSSMFKLKSGDKTFEADNTGSMSAN
                     QNDNGSIENSFFLQRINPDSTAQGKIVFDVSENIANAKDKKLEVISSLFSVKKITFDL
                     SDAKKTSKAKKDKQDTEVAAASSNSDNVSYEASATTPATTSSANNDSEENEQSSKDED
                     KQNASKSDKSSVEKSESNEETATVESAPQSKPVTSEAPPNQNNHNEDSMYDASTE"
     gene            1850..2476
                     /locus_tag="POB55_001987"
     CDS             1850..2476
                     /locus_tag="POB55_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500947.1"
                     /translation="MKFKAIVAIALSLSLLTACGANQHKENNSKSNDTNKKTQQTDNT
                     TQSNTDKQMTPQEAEDIVRNDYKARGANENQTLNYKTNLERSNEHEYYVEHLVRDAVG
                     TPLKRCAIVNRHNGTIINIFDDMSEKDKEEFEAFKKRSPKYNPGMSDHDETDGESEDI
                     QHHDNDNNKAIQNDIPDQKVDDKSDKNAVNKEEKHDNGTNNSEETKVK"
     gene            2517..2858
                     /locus_tag="POB55_001988"
     CDS             2517..2858
                     /locus_tag="POB55_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3969 family protein"
                     /protein_id="HDI6500948.1"
                     /translation="MITFENIQQLEKYTLMTMHGLFNQLKLGIISIDNAEHTLFTPYM
                     METLSSLGMKDSIVDLIHKGTELEDFAAFNLSIEDTVTVCLQRTEELLNQYKNVEFND
                     KILINWRIMQK"
     gene            2959..3531
                     /locus_tag="POB55_001989"
     CDS             2959..3531
                     /locus_tag="POB55_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500949.1"
                     /translation="MEKNEYIAKYNEYSQLLDATYSQAVAYLLNKYGAVTDDYYKEKS
                     YTRFLNGEIKSITKGKYTRAGEGLYCHHISEDKFQNLSDLRFISEFKYSYNYQKKENL
                     VYCDLIEHLILHAIITKESNGQFGVAGLCQMIKPTVIDWYISEYNPKPAWMQATKARA
                     YLPRILVEKLLIKIDDMLKEIEIYDFLESR"
     gene            complement(3729..4286)
                     /locus_tag="POB55_001990"
                     /pseudo
     CDS             complement(3729..4286)
                     /locus_tag="POB55_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864138.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
     gene            complement(4660..4836)
                     /locus_tag="POB55_001991"
     CDS             complement(4660..4836)
                     /locus_tag="POB55_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500950.1"
                     /translation="MDRLKYSLKVGILALLLFCTLNYLVPMQSNAFSIIIYSSIFAVL
                     LMLLVYIFLGILKK"
     gene            complement(4847..5230)
                     /locus_tag="POB55_001992"
     CDS             complement(4847..5230)
                     /locus_tag="POB55_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500951.1"
                     /translation="MSNQNLFDELEKKGYKLEDIFTKEEIKKYKAEDQLRAGKTQYVE
                     TGKDTATLYLSSAYTKTIAALGAGAISVISALTGGLVGAGVGGFLGSIAASNIDTSKG
                     VYIKLKTKKNAAGEYVLTGEKWGYQ"
     gene            complement(5834..5977)
                     /locus_tag="POB55_001993"
     CDS             complement(5834..5977)
                     /locus_tag="POB55_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDI6500952.1"
                     /translation="MTRERRSFSSEFKLQMVRLYKNGKPRNEIIREYDFTPSTFVNGG
                     YKM"
     gene            6180..6275
                     /gene="pepA1"
                     /locus_tag="POB55_001994"
     CDS             6180..6275
                     /gene="pepA1"
                     /locus_tag="POB55_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin
                     PepA1"
                     /protein_id="HDI6500953.1"
                     /translation="MMLIFVHIIAPVISGCAIAFFSYWLSRRNTK"
     gene            6398..6499
                     /locus_tag="POB55_001995"
     CDS             6398..6499
                     /locus_tag="POB55_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500954.1"
                     /translation="MQGFKEKHQELKKALCQIGLMRSISEVKQLNIA"
     gene            complement(6477..6653)
                     /locus_tag="POB55_001996"
                     /pseudo
     CDS             complement(6477..6653)
                     /locus_tag="POB55_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066885.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6847..7209)
                     /locus_tag="POB55_001997"
                     /pseudo
     CDS             complement(6847..7209)
                     /locus_tag="POB55_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036448.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
BASE COUNT         2656 a         1169 c         1040 g         2344 t
ORIGIN      
        1 acacttaata actccctctt aaatgcatcc aggttttatg tagtaaatca tgaaaataac
       61 atataaatat agaggagatt tacctttgaa tacagagaac aacaagaatc aaaaccgatc
      121 tgttaaaaat tctgaaagac gtggcatgtt aaaaggatgc ggcggttgcc ttatttcttt
      181 cattttatta ataatcttat tatcagcctg ttcaatgatg tttagtaata atgacaattc
      241 cactagtaat caatcatcaa aaacgcaatt aactcaaaaa gatgaaaata aaaatgaaga
      301 taagcctgag gaaaaatcag aaacagcaac agatgaggat ttacaatcaa ccgaagaagt
      361 acccgcaaat gaaaatactg aaaataatca acatgaaatt gatgaaataa caacaaaaga
      421 tcaatcagat gatgaaatta acacaccaaa cgttgcagaa gataaatcac aagacgactt
      481 gaaagatgat ttaaaagaaa agcaacaatc aagtaaccat catcaatcca cgcaacctaa
      541 gacctcacca tcaactgaaa caaacacgca acaatcattt gctaattgta agcaacttag
      601 acaagtatat ccgaatggtg tcactgccga tcatccagca tatcgaccac atttagatag
      661 agataaagat aaacgtgcat gtgaacctga taaatattaa acaacaagcg aattgaattc
      721 aaattgtatt tagctttatg cactaatcac atagtaaata atgagggaga ttttttaggc
      781 atgagcaatc aattcaaaag cgaagaagag cgaagacaat gggaacaatt ccaagctttc
      841 caaaatcaac aaaaccaaca gaaccagcaa tacggacaaa agaaatctaa aaaaggatgg
      901 ttctggggct gtggtggttg tctagtatta tttattttaa ttatcatcgg tatttcagct
      961 tgtacagctg gtattacagg taaccttggc ggaaatagtt ctaaagaaac gaacaaaacc
     1021 cataaaattg gagagactgt aaaaaatggt gatcttgaag tcacagttaa ttcagttgaa
     1081 actatgaaat cagttggtcc atctatcgcg ccaacaaatg ctaaaggtac atttgtcgtt
     1141 gctgatgtga cgattaaaaa caaaggtaaa gaagcgttaa caattgatag ttcaatgttt
     1201 aaactgaaat ctggtgataa aacatttgaa gcagataata caggctcaat gtctgctaat
     1261 caaaatgaca acggtagtat agaaaattca tttttcttac agcgtataaa tcctgatagc
     1321 actgctcaag gtaaaattgt tttcgatgtg tcagaaaaca tagccaacgc aaaagataaa
     1381 aaattagaag ttatttctag tttatttagc gtcaagaaga ttacatttga tttatccgat
     1441 gctaaaaaaa catcaaaagc taaaaaagac aagcaagata cagaagtagc tgctgcgagt
     1501 tcaaatagcg ataatgtaag ttatgaagct tcggctacta cacctgctac aacttctagt
     1561 gcaaataatg attctgaaga aaatgaacag tctagtaaag atgaagataa gcagaatgcg
     1621 tctaaaagtg ataaatctag tgtagaaaaa agtgaatcta atgaagaaac tgctactgta
     1681 gaatcagcac ctcaaagcaa acctgttaca agtgaagcac cacctaacca aaataatcac
     1741 aacgaagata gcatgtacga cgcttcaaca gaataaaatt tcagggtagc tcggctaccc
     1801 ttcttttacg gaaaaataag tatacatagc aatataagga gataaaaaaa tgaaattcaa
     1861 agctatcgtt gccattgcat tatcattgtc actattaact gcttgcggtg ctaatcaaca
     1921 taaagaaaat aatagtaaat caaatgacac taataaaaag acgcaacaaa ctgacaacac
     1981 tacacagtca aatacagaca agcaaatgac accacaagaa gccgaagata tcgttcgaaa
     2041 cgattacaaa gcaagaggtg ctaacgaaaa tcaaacatta aattataaaa caaatcttga
     2101 acgaagtaat gaacatgaat attatgttga acatctagtc cgcgatgcag ttggcacacc
     2161 tttaaaacgt tgcgctattg ttaatcgaca caatggtacg attattaata tttttgatga
     2221 tatgtcagaa aaagataaag aagaatttga agcatttaaa aagagaagcc ctaaatacaa
     2281 cccaggtatg agtgatcatg atgaaacaga tggtgagtca gaagacattc aacatcatga
     2341 caatgataat aacaaagcaa ttcaaaatga cataccagat caaaaagtcg atgataaaag
     2401 tgataaaaat gctgttaata aagaagaaaa acacgataac ggtacaaata attctgaaga
     2461 aactaaagtt aaataagggt atactttgat taatcgtaat ttttaggaga accgctatga
     2521 taacatttga aaatatacaa caacttgaaa aatacacctt aatgactatg catggtcttt
     2581 ttaatcaact taaactcggt attatttcaa tcgacaatgc agagcatacg ctctttacac
     2641 cttatatgat ggaaacactc tcttccctag gcatgaaaga cagcattgtc gatttaattc
     2701 ataaagggac tgaattagaa gactttgcag catttaattt atcaattgaa gatacagtta
     2761 cagtctgttt acaaagaact gaagaactat taaatcaata caaaaatgtg gaattcaatg
     2821 acaaaatatt aatcaattgg cgtattatgc aaaagtaata gacatataaa agtcgaacgt
     2881 aactaaatgg atattgattt tattctttct aaattacaag tatttcattt tataaagttt
     2941 gaaaagaggt atattgaagt ggagaaaaat gaatatatag ctaaatataa tgaatatagt
     3001 caattattag acgctacata ctcgcaagcc gtagcatacc ttttaaataa atatggcgct
     3061 gtaaccgatg actattataa ggaaaaatca tacacgcgat ttttaaatgg agaaatcaaa
     3121 agtattacaa aaggaaaata cactagagct ggtgaaggat tatattgtca tcacataagc
     3181 gaagacaaat tccaaaactt atctgaccta agattcattt ccgaatttaa gtactcatac
     3241 aactatcaaa agaaagaaaa cttagtttac tgcgatttaa tcgagcattt aattttacat
     3301 gcaattatta caaaagaatc caatggccaa tttggtgtgg ctggattatg tcaaatgatc
     3361 aaaccaacag tcattgattg gtacattagc gaatataatc caaaaccagc atggatgcaa
     3421 gccaccaaag cacgtgccta tttgcctaga atattagtag agaaactact cattaaaatt
     3481 gacgatatgt taaaagaaat agaaatatat gatttccttg agtctagata aatgattaat
     3541 ggagatttat tttttgctgt tgagattttg ttatagatgt ttaaacccat aattaaatat
     3601 attttataaa aatagaccac gcatacctat ctttaaacgg acaatgttta taaatgagtt
     3661 tgcatggtct tgaattgtat tagttttctt ttggttttaa taaatcgact agattttcac
     3721 aatatttatc aaatatgtat tcctaaatta tacagcctta atccagcagc tactttcgaa
     3781 acttccaact tagttgatat aaggttcaat agtttgtttc attctttttc agataaacca
     3841 gaacttaaat tgatattatt gacttcataa aaattataga ctaatgcctc tatttgcttt
     3901 tttggcataa gtaagtcgac tgaaaactga tttacgtcgc tttcataaat catttcatgt
     3961 aaattcttta gactattatc gttgttatct ttaattttgt ataaataacg gccgagttca
     4021 cgagctattg caaatcttgt attattaatc gagtgattat tattgatata aatcgttctt
     4081 ccacttaaat aacccgaagt attaccctcc attttaatat atttaacttt taaattaagt
     4141 tgaaataata gcttgtctat gtcaatagca aagtgttcag aagtaataaa aagttgatcc
     4201 attttgtcct taactaatgg ctgaattaat tgaactattt ctggttctaa aatatcttca
     4261 taatgaactt tctcaataac tttcaattga ttcccctccc taactaaaaa atcgaaccta
     4321 catcatttaa taaatttaaa actataggtg taaacattat taaaataaaa cccctccact
     4381 actaaatgta acggagggac atgatttcaa aataaaatgt cattttttat aaatttatta
     4441 taatattttt catcatactg tacaaccgaa tctaatccaa cgatcccgaa aagaggaaca
     4501 acggtggttc tgcttcataa tcacttttaa ctaagtgttc atttttaatt atttgatcga
     4561 cggttttcca attttttact tcttgctctt ttaaaaaaat ggctatgaaa aattctatcc
     4621 tcatagattt cttcatagcc aatgctaatt tttttcatgt catttcttca aaattcctaa
     4681 aaatatataa actaaaagca taagtaacac agcaaaaatt gatgaatata taattattga
     4741 aaaagcatta ctttgcattg gaactaaata atttaaagta caaaataata ataatgctaa
     4801 aattccaact ttaagtgaat attttaatct atccataaaa tcacccttac tgatatcccc
     4861 atttttctcc tgtcagaacg tattccccgg ctgcattctt tttagttttt aattttatat
     4921 atactccctt actagtatca atattcgatg cagctataga tccaaggaaa ccaccaacac
     4981 ctgcgccaac taaaccacct gttagtgcag aaataacgct aatagccccc gcacctaaag
     5041 cagctattgt ttttgtatat gcagaagaaa gatataatgt tgcagtatct ttacctgttt
     5101 ctacatattg agttttaccc gctcttaatt ggtcttcagc tttatatttt tttatttctt
     5161 cttttgtaaa tatatcttcc agtttataac cttttttctc aagttcatca aataaatttt
     5221 ggttactcaa atatattacc tttgcttgag aatggtctaa cttatcttca gcatgagcta
     5281 catctgaatt ataagataat gaaattggac taacaaataa tacaccagca gctaataata
     5341 aagattttta attcgttttt cattagtttc ttttagatga tttttgtatt tagatttcgt
     5401 ataaacagaa actagatttt ttcatgatcg acctatcttt tgtccagata cagtgagacc
     5461 cttgtcattt aaatgatttt taattcgtct tgtaccaaag acttttctat tagaattaaa
     5521 aatatttatg acggcatgtt ctatgtttga atcatcttta gtgattttat tatcttttct
     5581 ttttatagaa tcataatagg tacttcttag tattatcagg actttacaca ttgctgatac
     5641 tgaatattga tgtgcattct tttgaatgac ttctattttt gccccataat cagcgctact
     5701 tgctttaaaa tatcgttctc catttttaaa tgttgaactt ctttgcgtaa tttaatcagc
     5761 tctttttctt catccgataa gttatcttgg tgattgaatg tacccgtgtt ttgatgttgc
     5821 tttatccatt ttcctacatt ttataaccgc catttacaaa cgtcgaaggt gtgaaatcat
     5881 actcgcgtat aatttcattc ctaggcttac catttttata taatctaacc atttgtaact
     5941 taaactctga actaaatgat cttctttctc ttgtcataat aaaatcgcct actttcttaa
     6001 attaacaata tctattctca tagaatttgt ccaattaagt gtagacgatt caatctatca
     6061 gctagaatca tataacttat cagaagcaag tgactgtgct tgtatatttg ccgatgatat
     6121 aatagtagag tcgcctatct ctcaggcgtc aatttagacg cagagaggag gtgtataagg
     6181 tgatgcttat tttcgttcac atcatagcac cagtcatcag tggctgtgcc attgcgtttt
     6241 tttcttattg gctaagtaga cgcaatacaa aataggtgac atatagccgc accaataaaa
     6301 atcccctcac taccgcaaat agtgagggga ttggtgtata agtaaatact tattttcgtt
     6361 gtcttaatta tactgctaat ttttcttttt gtaaaatatg caaggtttta aagagaaaca
     6421 tcaagaacta aaaaaggctc tatgtcaaat tggactgatg cgttcaatat ccgaagttaa
     6481 gcaactaaac attgcttaac ttccttttta ctttttggag cgtaaagttt tgaacataat
     6541 aatattcgat tgcgcaaatg attgtaactt ccataaccaa aagatgtacg tttaattaat
     6601 tttattttgt tatttatacc ttctaaagga ccatttgata aattgtaata atcaatggtt
     6661 acactattaa aagtgtcaca aattcttatg aatctggcat aaactttgaa ttaactaaat
     6721 aagtaagaaa acctcggcac tttatcattt taatagtgtc gagattttta tagatactac
     6781 aaatatttat aacatagtta aactcatcta atgacttata tttttgtttc atcacaatat
     6841 gaacaattat ttattggacg tattttgctc tttttttatt tcagaaactg acttaggatt
     6901 tttattaaat tttctaccca attcatctgt ataagaaata tcggtatcaa attgaaaatc
     6961 atcaacagat cttataccag ctgtaaatga taacttttta tcattattta tataaccact
     7021 aatagaataa ccatccattg ggttttcttt aaaattagcg aagcttatat ttttataacc
     7081 tttcacattg tacttcatat aaatcgttat tctttctttt tgttcatcat attatttctt
     7141 ttcgtcatat ttcatcttca aaaagattcc gcctagaatc aatattaaaa ttataattac
     7201 aatcataag
//
LOCUS       DAMZKC010000124         7041 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.125, whole genome shotgun sequence.
ACCESSION   DAMZKC010000124 DAMZKC010000000
VERSION     DAMZKC010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7041
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.125"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            356..1036
                     /locus_tag="POB55_001998"
     CDS             356..1036
                     /locus_tag="POB55_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL1"
                     /protein_id="HDI6500955.1"
                     /translation="MKFKAIAKASLALGMLATGVITSNVQSVQAKTEVKQQSEADLKL
                     YYNGPSFEYKKVTGYGFIEGKDRFIDFIYNGQYNKISLVGSDKDKYNEEVNPDIDVFV
                     VREGNGRQADNHSIGGITKTNRGVYYDYIHTPILEIKKGKEEPQSSLYQIYKEDISLK
                     ELDFKLRKQLISQSGLYSNGLKQGQITITMNDGTTHTIDLSQKLEKERMGESIDGRQI
                     QKILVEMK"
     gene            1322..2017
                     /locus_tag="POB55_001999"
     CDS             1322..2017
                     /locus_tag="POB55_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL2"
                     /protein_id="HDI6500956.1"
                     /translation="MKMKSIVKISLLLGILATGVNTTTEKPVHAEKKPIVISENSKKL
                     KAYYTQPSIEYKNVTGYISFIQPSIKFMNIIDGNSVNNIALIGKDKQHYHTGVHRNLN
                     IFYVNEDKRFEGAKYSIGGITSANDKAVDLIAEARVIKADHIGEYDYDFFPFKIDKEA
                     MSLKEIDFKLRKYLIDNYGLYGEMSTGKITVKKKYYGKYTFELDKKLQEDRMSDVINV
                     TDIDRIEIKVRKA"
     gene            2308..3378
                     /locus_tag="POB55_002000"
     CDS             2308..3378
                     /locus_tag="POB55_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL3"
                     /protein_id="HDI6500957.1"
                     /translation="MKMRTITKTSLALGLLTTGAITVTTQSVKAEKIQSTKVDKVPTL
                     KAERLAMINITAGANSATTQAANTRQERTPKLEKAPNTNEEKTSASKIEKISQPKQEE
                     QKTLNISATPAPKQEQSQTTTESTTQQTKMTTPPSTNTPQPMQSTKSDTPQSPTIKQA
                     QTDMTPKYEDLRAYYTKPSFEFEKQFGFLLKPWTTVRFMNVIPNRFIYKIALVGKDEK
                     KYKDGPYDNIDVFIVLEDNKYQLKKYSVGGITKTNSKKVNHKVELSITKKDNQGMISR
                     DVSEYMITKEEISLKELDFKLRKQLIEKHNLYGNMGSGTIVIKMKNGGKYTFELHKKL
                     QEHRMADVIDGTNIDNIEVNIK"
     gene            3497..3634
                     /locus_tag="POB55_002001"
     CDS             3497..3634
                     /locus_tag="POB55_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500958.1"
                     /translation="MFVKVAFLCLKSDETSNVPSVESHQNHFYLTNIMDFLIYLTMIQ
                     I"
     gene            3743..4588
                     /locus_tag="POB55_002002"
     CDS             3743..4588
                     /locus_tag="POB55_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL4"
                     /protein_id="HDI6500959.1"
                     /translation="MKITTIAKTSLALGLLTTGVITTTTQAANATTPPSTKVETPQQV
                     ANATTPSSTKVEAPQQAANATTPPSSNVDTSPPQSPTTKQVPTEINPKFKDLRAYYTK
                     PSLEFKNEIGIILKKWTTIRFMNVVPDYFIYKIALVGKDDKKYGEGVHRNVDVFVVLE
                     ENNYNLEKYSVGGITKSNSKKVDHKAGVRITKEDNKGTISHDVSEFKITKEQISLKEL
                     DFKLRKQLIEKNNLYGNVGSGKIVIKMKNGGKYTFELHKKLQENRMADVIDGTNIDNI
                     EVNIK"
     gene            4952..5656
                     /locus_tag="POB55_002003"
     CDS             4952..5656
                     /locus_tag="POB55_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL5"
                     /protein_id="HDI6500960.1"
                     /translation="MKMAAIAKASLALGILATGTITSLHQTVNASEHEAKYENVTKDI
                     FDLRDYYSGASKELKNVTGYRYSKGGKHYLIFDKHQKFTRIQIFGKDIERFKARKNPG
                     LDIFVVKEAENRNGTVFSYGGVTKKNQDAYYDYINAPRFQIKRDEGDGIATYGRVHYI
                     YKEEISLKELDFKLRQYLIQNFDLYKKFPKDSKIKVIMKDGGYYTFELNKKLQTNRMS
                     DVIDGRNIEKIEANIR"
     gene            6102..6800
                     /locus_tag="POB55_002004"
     CDS             6102..6800
                     /locus_tag="POB55_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL6"
                     /protein_id="HDI6500961.1"
                     /translation="MKLKALAKATLVLGLLATGVITTESQTVKAAESTQGQHNYKSLK
                     YYYSKPSIELINVDGLYRQHLTDKGAYVWKNLKDYYIGLLGEDSKKFKSDVYGDLDAF
                     LVIEEEPVKGRQYSIGGISKTNSKEFKEREVDVKVTRKADRDTTSTKDSKFKITKEEI
                     SLKELDFKLRQKLMKEENLYDAINHRKGKIVVKMEDDKFYTFELTKKLQPHRMGDTID
                     GTKIKEINVELEYK"
BASE COUNT         2869 a          939 c         1246 g         1987 t
ORIGIN      
        1 cgtatatgtt attaatgttg aaaataccgt aatgacgcgt tttagttgat gtgtatcact
       61 aatatctttg gaaattttaa tcaggtacta cgacaatatg atgtctgttt tgtgtctgaa
      121 agttttacag tttttaaaat aaaaatggta taaagtgtga tttgtataaa aaagagtctc
      181 gacggataag aattgattaa taacagttag cattttatta attaccttaa caatgattca
      241 agtttagtta aatgaggttt aatttgaaag gggatagcgc ctcaatataa tgtaggtaga
      301 ttgttcatat tacgtaattg aaaaatcaaa tttaaataga ttggggctaa aaattatgaa
      361 atttaaagcg atagcaaaag caagtttagc attgggaatg ttagcaacag gtgtaattac
      421 atcgaatgta caatcagtac aagcgaaaac agaagttaaa caacaaagtg aggctgattt
      481 aaaactttat tataatggac caagttttga atataaaaaa gtaactggat atggatttat
      541 tgaaggtaaa gatagattta ttgattttat atacaatgga caatataata aaatatcttt
      601 agttggttct gataaagata aatataatga agaagttaac ccagatatag atgtgtttgt
      661 cgttagagaa ggaaacggta gacaagctga taatcattcg attggtggca taacaaaaac
      721 taatagagga gtgtattatg actatataca cacaccaatc cttgaaatca agaaaggtaa
      781 agaagaacca caaagtagtc tataccaaat ttataaagaa gacatctcac taaaagaact
      841 tgattttaaa ttaagaaagc aattaattag tcaaagtggc ttgtattcaa atggtcttaa
      901 acaaggtcaa attacaatta caatgaatga tggcacaaca catacaatcg atttaagtca
      961 aaaacttgaa aaagaacgta tgggcgagtc tatcgatggc agacaaatac aaaaaattct
     1021 agtagaaatg aaataatact ttctaacaac aaagcgctat gttgaatagt gcttgttatg
     1081 gaaatatatg gaagttaagc gacgtactgt tgcttagctt ctttttttga ggggaaaagt
     1141 tacaaaactc acacaaacag tcgcaccacg cattatcttt tgcttaaata gcttaatcat
     1201 attttatgaa tagttaaaaa caggttaatg tgaatatctg aatacagctc ctataatatg
     1261 ggtgtatggt tcaaattacg taataaaaca atctaattat aatagattgg agcatacagc
     1321 tatgaaaatg aaatcaattg taaaaataag tttgttatta ggaatattag caacaggtgt
     1381 aaacactaca acggaaaaac cagttcatgc cgaaaagaaa cctattgtaa taagtgaaaa
     1441 tagcaaaaaa ttaaaagctt attatactca acctagtatt gaatataaaa atgtgacagg
     1501 ttatatcagt ttcattcaac caagtattaa atttatgaat atcatagatg gtaattctgt
     1561 taataatatt gctttaattg gcaaagataa gcaacattat catacgggtg tacatcgtaa
     1621 tcttaatata ttttacgtta atgaggataa gagatttgaa ggtgcaaagt actccattgg
     1681 cggtatcacg agtgcaaacg ataaagctgt cgacctaata gcagaagcaa gagttattaa
     1741 agcagatcat attggtgaat atgattatga ctttttccca tttaaaatag ataaagaagc
     1801 aatgtcattg aaagagattg attttaaatt aagaaaatac cttattgata attatggtct
     1861 ttacggtgaa atgagtacag ggaaaattac cgtcaaaaag aaatactacg gaaagtatac
     1921 atttgaattg gataaaaagt tacaagaaga ccggatgtcc gatgttatca atgtcacaga
     1981 tattgataga attgaaatca aagttagaaa agcataacac acatacttga cgacgaaata
     2041 atttgaaatt gaaatagaga ggttaagtga cgatcaaacg ttgcttaact tctttttaat
     2101 gcttaaaaat catttcaaag gcacatagaa acgctatatt aacctcataa tcactcatta
     2161 ttttttgctt aaattactta ataatacttc aataattgtt aaaaggggtt taatgtgatt
     2221 atcttagaat gccatctata atgatgttgt atgattcaaa ttacgtaaaa agacaatcga
     2281 atataatata gattggagca tacaattatg aaaatgagaa caattactaa aaccagttta
     2341 gcactagggc ttttaacaac aggcgcaatt acagtaacga cgcaatcggt caaagcagaa
     2401 aaaatacaat caactaaagt tgacaaagta ccaacgctta aagcagagcg attagcaatg
     2461 ataaacataa cagcaggtgc aaattcagcg acaacacaag cagctaacac aagacaagaa
     2521 cgcacgccta aactcgaaaa ggcaccaaat actaatgagg aaaaaacctc agcttccaaa
     2581 atagaaaaaa tatcacaacc taaacaagaa gagcagaaaa cgcttaatat atcagcaacg
     2641 ccagcgccta aacaagaaca atcacaaacg acaaccgaat ctacaacgca gcaaactaaa
     2701 atgacaacac ctccatcaac aaacacgcca caaccaatgc aatctactaa atcagacaca
     2761 ccacaatctc caaccataaa acaagcacaa acagatatga ctcctaaata tgaagattta
     2821 agagcgtatt acacgaaacc gagttttgaa tttgaaaagc agtttggatt tttgctcaaa
     2881 ccatggacga cggttaggtt tatgaatgtt attccaaata ggttcatcta taaaatagct
     2941 ttagttggaa aagatgagaa aaaatataaa gatggacctt acgataatat cgatgtattt
     3001 atcgttttag aagacaataa atatcaattg aaaaaatatt ctgtcggtgg catcacgaag
     3061 actaatagta aaaaagttaa tcacaaagta gaattaagca ttactaaaaa agataatcaa
     3121 ggtatgattt cacgcgatgt ttcagaatac atgattacta aggaagagat ttccttgaaa
     3181 gagcttgatt ttaaattgag aaaacaactt attgaaaaac ataatcttta cggtaacatg
     3241 ggttcaggaa caatcgttat taaaatgaaa aacggtggga aatatacgtt tgaattacac
     3301 aaaaaactgc aagagcatcg tatggcagac gtcatagatg gcactaatat tgataacatt
     3361 gaagtgaata taaaataatc atgacattct ctaaatagaa gctgtcatcg gaaaaacaag
     3421 aagttaagtg acaacggttt acatgttgct tagcttcttt tattatgcgt aatgatgtaa
     3481 aaagacgaat attcatttgt ttgtaaaagt ggcatttcta tgtcttaaaa gtgacgaaac
     3541 ttcaaatgtg ccaagtgttg aatcacatca aaatcatttt tatttaacga acattatgga
     3601 tttcttaatt tacttaacga tgattcaaat atagttaaac aaggtttaat gtgaatggag
     3661 caatgcgcca tctataataa agctgtatga ttcaatgaat gtaatcgaac aaatctaata
     3721 attacgaatg gagcatacaa ctatgaaaat aacaacaatt gctaaaacaa gtttagcact
     3781 aggcctttta acaacaggtg taatcacaac gacaacgcaa gcagcaaatg cgacaacacc
     3841 gccttcaact aaagtggaaa caccgcaaca agtagcaaat gcaacaacac catcttcaac
     3901 taaagtggaa gcaccgcaac aagcagcaaa cgcgacaaca ccaccttcgt ctaatgtaga
     3961 cacatcacca ccacaatcgc caaccacaaa acaagtacca acagaaataa atcctaaatt
     4021 taaagattta agagcgtatt atacgaaacc aagtttagaa tttaaaaatg agattggtat
     4081 tattttaaaa aaatggacga caataagatt tatgaatgtt gtcccagatt atttcatata
     4141 taaaattgct ttagttggta aagatgataa aaaatatggt gaaggagtac ataggaatgt
     4201 cgatgtattt gtcgttttag aagaaaataa ttacaatctc gaaaaatatt ctgtcggtgg
     4261 tatcacaaag agtaatagta aaaaagttga tcacaaagca ggagtaagaa ttactaagga
     4321 agataataaa ggtacaatct ctcatgatgt ttcagaattc aagattacta aagaacagat
     4381 ttccttgaaa gaacttgatt ttaaattgag aaaacaactt attgaaaaaa ataatctgta
     4441 cggtaacgtt ggttcaggta aaattgttat taaaatgaaa aacggtggaa agtacacgtt
     4501 tgaattgcac aaaaaattac aagaaaatcg catggcagat gtcatagatg gcactaatat
     4561 tgataacatt gaagtgaata taaaataatc atgacattct ctaaatagaa gctgtcatcg
     4621 gaaaaacaag aagttaagtg acaacggcct acatgttgct tagcttcttt tgttatgttc
     4681 gatgatttga gaacccgaat tttcgatggg tccaaatatg acgtggaaga gtcctgaatt
     4741 tatctgtaaa tccctatcta tcgggtgtgg agcacaacgg aatcagtttt atttaacgaa
     4801 cattatagat tccttaattt acttaataat gattcaatga ttattaaaca tggtttaatg
     4861 tgaaaggtca aatacgccaa ttataataaa gctgtatgat tcaatagacg taagcgaaca
     4921 aatctaataa ttacgaatgg agcatacaac tatgaaaatg gcagcaattg cgaaagcaag
     4981 tttagcatta ggtattttag caacaggaac aataacgtca ttgcatcaaa ctgtaaatgc
     5041 gagtgaacat gaagcaaaat atgaaaatgt gacaaaagat atctttgact taagagatta
     5101 ctatagtggc gcaagtaagg aacttaaaaa tgttactggt tatcgttata gcaaaggtgg
     5161 caagcattac cttatctttg ataaacatca aaagttcact agaatacaaa tttttggtaa
     5221 agatatagaa agatttaaag cacgcaaaaa tccgggatta gacatatttg ttgttaaaga
     5281 agcggaaaac cgtaatggca cagtgttttc atatggtggt gtcactaaga aaaatcaaga
     5341 cgcttattat gattatataa acgcaccaag atttcaaatc aagagagatg aaggtgacgg
     5401 tattgctacg tacggtagag tacactacat ttataaagaa gagatttcac ttaaagaact
     5461 cgactttaaa ttgagacagt atttaattca aaattttgat ctgtataaaa agtttcctaa
     5521 agatagtaag ataaaagtga taatgaaaga tggcggctat tatacgtttg aacttaataa
     5581 aaaattacaa acaaatcgca tgagtgacgt cattgacggt agaaatattg aaaaaataga
     5641 agccaacatt agataatgca atgaaatatg gataatagta aaatatggat agtatagagg
     5701 agttaggcaa cataagttgc ttagcttctt ttttgtgttg gagagatgaa aaagaagcgt
     5761 atcgatgaat aataaaaaca ccaataaaac ttgtgggaat agttgatacc tatagtcgcg
     5821 cgttgtcctt ttcgtgacat gaaacaatgt ggaaaacata attaaattga gggaaagtgt
     5881 gaatagttaa aaaagctgcg ttaagtttaa aaaatagatt aacgctgtta ggattctatt
     5941 aattagctta acattggttc aaaaatagtt aaaaagaggt taattcatag cttagtattc
     6001 cgcttatata atgatagtag attgttcgta ttacgtaatt gaaataatca tataaaaata
     6061 tattaagaca aaatttataa atagattggg agaatagtac tatgaaatta aaagcgttag
     6121 ctaaagcaac attagtattg ggattgttag ctactggtgt aataacaaca gaaagtcaaa
     6181 cagtaaaagc ggcagaatca actcaaggtc aacacaatta taaatcatta aaatactact
     6241 atagcaagcc aagtatagag ttaataaatg tagatggtct gtatagacaa catttaactg
     6301 ataaaggtgc atatgtatgg aaaaatctta aagattatta tattgggcta ctaggtgaag
     6361 atagtaagaa attcaaatca gatgtatacg gggacctaga tgcattttta gtcatagaag
     6421 aagaacctgt taaaggaaga caatattcaa ttggcggtat aagtaagaca aatagtaaag
     6481 aatttaaaga aagagaagtc gatgttaaag taacaagaaa agcagacaga gatactacat
     6541 caactaaaga tagtaaattt aaaattacaa aagaagaaat ctcgttaaaa gagttagatt
     6601 ttaaattaag acaaaaattg atgaaagaag agaatttata cgatgcaatt aaccatagaa
     6661 aaggtaaaat tgtagttaaa atggaagatg ataagtttta tactttcgaa cttacaaaaa
     6721 aattacaacc gcatcgcatg ggtgacacga tagatggtac caaaatcaaa gaaattaatg
     6781 ttgagctaga atataaataa tctttggaca agcagactag taattgtagg gaagttaagc
     6841 gataacatat tgcttagctt cttttttgtt ttgttatgat gaaaaaagga gcgggtttat
     6901 gatcaagttt ttggaaaaac ggttgatact tatagtcgcg cgttgtcctt ttcgtgacat
     6961 gaaacaatgt ggaaaacata attaaattga gggaaagtgt gaatagttaa aaaattagta
     7021 ttgtgttata aaaaataatt a
//
LOCUS       DAMZKC010000125         6992 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.126, whole genome shotgun sequence.
ACCESSION   DAMZKC010000125 DAMZKC010000000
VERSION     DAMZKC010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6992
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.126"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            158..272
                     /locus_tag="POB55_002005"
                     /pseudo
     CDS             158..272
                     /locus_tag="POB55_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499947.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            552..1211
                     /gene="arlR"
                     /locus_tag="POB55_002006"
     CDS             552..1211
                     /gene="arlR"
                     /locus_tag="POB55_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor ArlR"
                     /protein_id="HDI6500962.1"
                     /translation="MTQILIVEDEQNLARFLELELTHENYNVDTEYDGQDGLDKALSH
                     YYDLIILDLMLPSINGLEICRKIRQQQSTPIIIITAKSDTYDKVAGLDYGADDYIVKP
                     FDIEELLARIRAILRRQPQKDIIDVNGITIDKNAFKVTVNGAEIELTKTEYDLLYLLA
                     ENKNHVMQREQILNHVWGYNSEVETNVVDVYIRYLRNKLKPYDRDKMIETVRGVGYVI
                     R"
     gene            1208..2563
                     /gene="arlS"
                     /locus_tag="POB55_002007"
     CDS             1208..2563
                     /gene="arlS"
                     /locus_tag="POB55_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase ArlS"
                     /protein_id="HDI6500963.1"
                     /translation="MTKRKLRNNWIIVTTMITFVTIFLFCLIIIFFLKDTLHNSELDD
                     AERSSSDINNLFHSKPVKDISALDLNASLGNFQEIIIYDEHNNKLFETSNDNTVRVEP
                     GYEHRYFDRVIKKRYKGIEYLIIKEPITTQDFKGYSLLIHSLENYDNIVKSLYIIALA
                     FGVIATIITATISYVFSTQITKPLVSLSNKMIEIRRDGFQNKLQLNTNYEEIDNLANT
                     FNEMMSQIEESFNQQRQFVEDASHELRTPLQIIQGHLNLIQRWGKKDPAVLEESLNIS
                     IEEMNRIIKLVEELLELTKGDVNDISSEAQTVHINDEIRSRIHSLKQLHPDYQFDTDL
                     TSKNLEIKMKPHQFEQLFLIFIDNAIKYDVKNKKIKVKTRLKNKQKIIEITDHGIGIP
                     EEDQDFIFDRFYRVDKSRSRSQGGNGLGLSIAQKIIQLNGGSIKIKSEINKGTTFKII
                     F"
     gene            2847..5645
                     /locus_tag="POB55_002008"
     CDS             2847..5645
                     /locus_tag="POB55_002008"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HDI6500964.1"
                     /translation="MTNERKEVSEAPVNFGANLGLMLDLYDDFLQDPSSVPEDLQVLF
                     STIKNDDSIVPALKSTSSQNSDGTIKRVMRLIDNIRQYGHLKADIYPVNPPKRKHVPK
                     LEIEDFDLDQQTLEGISAGIVSDHFADIYDNAYEAILRMEKRYKGPIAFEYTHINNNT
                     ERGWLKRRIETPYKVTLNNNEKRALFKQLAYVEGFEKYLHKNFVGAKRFSIEGVDALV
                     PMLQRTITIAAKEGIKNIQIGMAHRGRLNVLTHVLEKPYEMMISEFMHTDPMKFLPED
                     GSLQLTAGWTGDVKYHLGGIKTTDSYGTMQRIALANNPSHLEIVAPVVEGRTRAAQDD
                     TQRAGAPTTDHHKAMPIIIHGDAAYPGQGINFETMNLGNLKGYSTGGSLHIITNNRIG
                     FTTEPIDARSTTYSTDVAKGYDVPIFHVNADDVEATIEAIDIAMEFRKEFHKDVVIDL
                     VGYRRFGHNEMDEPSITNPVPYQNIRKHDSVEYVFGKKLVNEGVISEDEMHSFIEQVQ
                     KELRQAHDKINKADKMDNPDMEKPADLALPLQADEQSFTFDHLKEINDALLTYPDGFN
                     ILKKLNKVLEKRHEPFNKEDGLVDWAQAEQLAFATILQDGTPIRLTGQDSERGTFSHR
                     HAVLHDEQTGETYTPLHHVPDQKATFDIHNSPLSEAAVVGFEYGYNVENKKSFNIWEA
                     QYGDFANMSQMIFDNFLFSSRSKWGERSGLTLFLPHAYEGQGPEHSSARLERFLQLAA
                     ENNCTVVNLSSSSNYFHLLRAQAASLDSEQMRPLVVMSPKSLLRNKTVAKPIDEFTSG
                     GFEPILTESYQADKVTKVILATGKMFIDLKEALAKNPDESVLLVAIERLYPFPEEEIE
                     ALLAQLPNLEEVSWVQEEPKNQGAWLYVYPYVKVLVADKYDLSYHGRIQRAAPAEGDG
                     EIHKLVQNKIIENALKNN"
     gene            5659..6927
                     /gene="sucB"
                     /locus_tag="POB55_002009"
     CDS             5659..6927
                     /gene="sucB"
                     /locus_tag="POB55_002009"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HDI6500965.1"
                     /translation="MPEVKVPELAESITEGTIAEWLKNVGDSVEKGEAILELETDKVN
                     VEVVSEEAGVLSEQLASEGDTVEVGQAIAIIGEGSGNASKENSNDNTPQQNEETNNKK
                     EETTNNSVDKAEVNQANDDNQQRINATPSARRYARENGVNLAEVSPKTNDVVRKEDID
                     KKQQAPASTQTTQQASAKEEKKYNQYPTKPVIREKMSRRKKTAAKKLLEVSNNTAMLT
                     TFNEVDMTNVMELRKRKKEQFMKDHDGTKLGFMSFFTKASVAALKKYPEVNAEIDGDD
                     MITKQYYDIGVAVSTDDGLLVPFVRDCDKKNFAEIEAEIANLAVKAREKKLGLDDMVN
                     GSFTITNGGIFGSMMSTPIINGNQAAILGMHSIITRPIAIDQDTIENRPMMYIALSYD
                     HRIIDGKEAVGFLKTIKELIENPEDLLLES"
BASE COUNT         2649 a         1007 c         1269 g         2067 t
ORIGIN      
        1 tttgaatatt ttttaagaat agtaaataaa acgcatgatt acgctatttt agaaaataaa
       61 aaaatttgta tttctcatta gaattagaat atttaaaagt tatgaggttt aaaaattata
      121 ttgtttacat actccttttg aatttataca ttatgaaatg ttacttccaa gttcaaaatc
      181 gcacattgaa atgatgtgtg aaaatgttta aactacggtc attttgtgaa aataaagata
      241 ataactattc attttacatt agtgaaaagt aagtatacga caacaattga tattgcggta
      301 aggccttgtg ttacagtatt ctatatttaa gtactgcaat cagaattaac agaattccat
      361 taactgatta ttaaatattt gagttaataa ataattaatg attgtagctt gaaaaattta
      421 aaacatggtt attgatttgt gataaaattt aaacgtaaac aaactaattt aaaaagcaac
      481 tattgtatag aaaaatacaa aatttaaaat atattacctt attagaaaaa gtcgtaatat
      541 gaggtgtaca aatgacgcaa attttaatag tagaagatga acaaaactta gcaagatttc
      601 ttgaattgga actcacacat gaaaattaca atgtggacac agagtatgat ggacaagacg
      661 gtttagataa agcgcttagc cattactatg atttaatcat attagattta atgttgccgt
      721 caattaatgg cttagaaatt tgtcgcaaaa ttagacaaca acaatctaca cctatcatta
      781 taattacagc gaaaagtgat acgtatgaca aagttgctgg gcttgattac ggtgcagacg
      841 attatatagt taagccattt gatattgaag aacttttagc aagaatacgt gcgattttac
      901 gtcgtcagcc acaaaaagat attatcgatg tcaatggtat tacaattgat aagaatgctt
      961 ttaaagtgac ggtaaatggc gcagaaattg aattaacaaa aacagagtat gatttactat
     1021 atcttcttgc tgaaaataaa aaccatgtta tgcaacggga acaaatttta aatcatgtat
     1081 ggggttataa tagtgaagta gaaacaaatg tcgtagatgt ttatataaga tatttacgaa
     1141 acaaattaaa accatacgat cgtgacaaaa tgattgaaac agttcgtggc gttgggtatg
     1201 tgatacgatg acaaaacgta aattgcgcaa taactggatt attgttacca cgatgattac
     1261 gtttgtcacg atatttttgt tttgtttaat tattattttt ttcttgaaag atacactgca
     1321 taatagtgag cttgatgatg cagaacgaag ctcaagcgat attaataatt tatttcattc
     1381 taagcctgtt aaagatatat ctgcattaga cttgaatgca tctttaggta attttcaaga
     1441 gataattatt tatgatgagc ataataataa attatttgag acatcgaatg ataacacagt
     1501 gagagttgaa ccaggttatg aacaccgtta ttttgaccgc gtaataaaaa aacgctataa
     1561 aggcattgaa tatttaatta ttaaagaacc aattacaacg caagatttca aagggtatag
     1621 cttgttaatt cattcactag aaaattatga taacatcgta aaatcattgt atatcattgc
     1681 gctggcattt ggagtgattg caacaattat aactgccaca atcagttatg tattttcaac
     1741 acaaattact aaaccgcttg tcagtttatc aaataaaatg attgagattc gacgagatgg
     1801 ttttcaaaat aaattgcaat taaatacaaa ttatgaagaa atagataatt tagcaaatac
     1861 gtttaatgag atgatgagcc aaattgaaga atcatttaat caacaaagac aatttgttga
     1921 agatgcgtca catgaattac gaacaccatt acaaattatt caaggtcatt taaatttgat
     1981 tcagcgatgg ggaaaaaaag acccagcagt attagaagaa tcgttaaata tttctattga
     2041 agaaatgaat cgtatcataa aattagtcga agaattactt gaattgacta aaggagatgt
     2101 aaatgacatt tcttctgaag cacagaccgt gcatattaat gatgaaattc gctcgcgaat
     2161 acactcatta aaacaattgc atcctgatta tcaatttgat acggatctga catctaaaaa
     2221 tctagaaatt aaaatgaaac ctcatcaatt cgaacaatta tttttaatct ttattgataa
     2281 tgcaatcaaa tatgatgtga agaataagaa aattaaagtt aagacaaggt taaaaaataa
     2341 gcaaaaaata attgaaatta cagatcatgg aattggtatt ccagaggaag atcaagattt
     2401 catttttgat cgcttttatc gagtggataa atctcgttca agaagtcaag gcggtaatgg
     2461 actcggatta tctattgctc aaaaaatcat tcaattaaac ggaggatcga ttaaaattaa
     2521 aagtgaaatt aacaaaggaa caacgtttaa aatcatattt taatcatgac tgagacgtca
     2581 atcaaagtca taggatcaat tttttaagta cacattagct gtgactaatg tataagaaca
     2641 actataaaac aaataaacag tggttcttta tcatttctgt tgtactccca aaatttacaa
     2701 taaaatacat ctataaacct agaagaatca acgcttttgt tgattcttct ttttagcaga
     2761 taaataggta aatctacttt aacaaataac taaatagtga tattattaca ttgtaagcgt
     2821 ttcaacattt ttgtggaggg tgtaaaatga ctaacgaaag aaaagaagtt tcagaggctc
     2881 ctgtaaactt cggtgcgaat ttaggtctaa tgttagatct atatgatgac tttttacaag
     2941 atccatcatc tgtaccagaa gatttacaag tcttattcag cacaattaag aatgatgact
     3001 caattgtacc agctttaaaa agtacaagta gtcaaaatag cgacggcaca attaagcgtg
     3061 tcatgcgttt aattgataat attcgccaat acgggcatct taaagccgat atttatcctg
     3121 taaatcctcc aaaaaggaaa catgtaccta aattagagat tgaagacttt gatttagatc
     3181 aacagacttt ggaaggtata tcagcaggaa ttgtttcaga tcactttgcc gacatttatg
     3241 ataatgctta tgaagcaatt ttaagaatgg aaaaacgtta caaaggacca attgcatttg
     3301 agtatacaca tattaataac aataccgaac gtggttggtt aaaaagaaga attgaaacgc
     3361 catataaagt aacgttaaat aataacgaaa aaagggcact attcaaacaa ttagcgtatg
     3421 ttgaagggtt tgaaaaatat cttcataaaa acttcgttgg tgcaaagcgt ttttcaattg
     3481 aaggggtaga cgcacttgta ccgatgttac aacgtactat tacgattgct gcgaaagaag
     3541 gtattaaaaa tatacaaata ggcatggctc accgtggacg tttaaacgtt ttaacgcatg
     3601 tcttagaaaa accgtacgaa atgatgattt cagaatttat gcatacagat ccaatgaaat
     3661 tcttacctga agatggtagc ttgcagttaa ctgctggatg gactggtgat gtgaaatatc
     3721 accttggtgg cattaaaact actgattcat acggtacaat gcagcgtatt gcactggcta
     3781 acaatccaag tcacttggaa attgttgcac ctgttgttga ggggcgtacg agagcagcac
     3841 aagatgatac acaacgagct ggggctccga cgactgatca tcataaagca atgccaatta
     3901 ttatacatgg cgatgctgct tatcctggtc aaggaattaa cttcgaaaca atgaacttag
     3961 gaaacttgaa aggctattct acgggtggtt cattgcatat tattactaac aatagaattg
     4021 gatttactac agaaccaatt gatgcacgtt caacaactta ttctacagat gtggccaaag
     4081 gttatgatgt gccaatattc catgtcaatg cagatgacgt tgaagctact attgaagcaa
     4141 ttgatattgc aatggaattt agaaaagagt ttcataaaga cgtcgttatt gatttagtag
     4201 gttatcgtcg tttcggacat aacgaaatgg atgaaccatc aattactaat ccagttcctt
     4261 atcagaatat tcgcaaacat gactctgttg aatatgtgtt tggtaaaaag cttgttaatg
     4321 aaggtgtcat ttcagaagat gaaatgcatt catttataga acaagtccaa aaggaactaa
     4381 gacaagctca tgataaaatt aataaagctg ataaaatgga taatccagat atggaaaagc
     4441 ctgcagatct tgcattaccg ttacaagcag acgaacaatc atttactttt gatcacttga
     4501 aagaaataaa tgatgcattg ttaacatatc cggatggctt taacattttg aaaaagttaa
     4561 acaaagttct tgagaagcgt catgagccgt ttaataaaga agatggttta gttgattggg
     4621 cacaagcaga acaacttgca tttgcgacaa ttttacaaga tggtacaccg attcgcttaa
     4681 ctggtcaaga tagtgaacgt ggtacattca gtcataggca tgccgtgtta catgatgagc
     4741 aaacaggtga aacatataca cctttacatc atgttcctga tcaaaaagcg acatttgata
     4801 tacacaattc tccgctttca gaagcagcag tagttggttt tgaatacggc tataatgtgg
     4861 aaaacaaaaa aagcttcaat atttgggaag cacaatatgg tgattttgca aatatgtcac
     4921 aaatgatttt tgacaacttc ttattcagtt ctcgctcaaa atggggagaa cgttcaggat
     4981 taacattatt cttacctcat gcatatgagg gtcaagggcc tgaacattca tcagcaagat
     5041 tagagcgatt tttacaatta gctgctgaaa ataattgcac agttgtcaac ttatctagtt
     5101 caagtaatta tttccactta ttgcgtgcac aagcggctag tttagattct gaacaaatgc
     5161 gaccattggt tgttatgtca ccaaaaagct tactgagaaa taaaacagtt gcaaaaccaa
     5221 ttgatgaatt tacttctggt ggatttgagc caattttgac agaatcatat caagcggata
     5281 aggttacaaa agttattttg gcaactggta aaatgttcat tgatttaaaa gaagcattag
     5341 ctaaaaatcc agacgaatca gtattactcg ttgcgattga aagattgtat ccattcccag
     5401 aggaagagat tgaagcatta ctagcacaat tgccaaacct tgaagaagtg tcatgggtac
     5461 aagaagaacc taaaaatcaa ggtgcctggt tatatgtcta tccatatgtt aaagtgctag
     5521 ttgcagataa atatgattta agttatcatg gcagaattca aagggctgct ccagctgaag
     5581 gcgatggaga aattcataaa cttgttcaaa ataaaattat agaaaatgca ttaaaaaata
     5641 actaggggga aataagtcat gccagaggtt aaagttccag aattagcaga atctattaca
     5701 gaaggtacca ttgcagaatg gttgaaaaac gtaggggata gcgtagaaaa aggtgaagct
     5761 attcttgaat tagaaactga taaagttaat gtcgaagttg tatctgaaga agcaggtgta
     5821 ttatctgaac aacttgcaag tgaaggcgac actgtagaag ttggacaagc aattgctatc
     5881 atcggcgaag gtagtggcaa tgcttctaaa gaaaatagta acgacaatac tccacaacaa
     5941 aatgaagaaa caaataataa aaaagaagaa acaacaaata attcggtaga taaagctgaa
     6001 gtaaatcaag caaatgatga caatcagcaa cgtattaatg ctacgccttc tgcgcgtcga
     6061 tatgctcgtg aaaatggtgt gaatcttgct gaagtaagtc cgaaaacaaa tgatgtggtt
     6121 cgtaaagaag atattgataa gaaacaacag gcaccggcat caacacaaac aacacaacaa
     6181 gcatctgcaa aagaagagaa aaaatacaat caatatccta caaaaccagt gattcgtgaa
     6241 aaaatgtcac gtagaaagaa aacagctgcc aaaaaattat tagaggtatc taataataca
     6301 gctatgttaa caacatttaa cgaagttgac atgacaaatg ttatggaatt gcgtaaacgt
     6361 aagaaagaac aatttatgaa agatcatgat ggtactaaat taggatttat gtcattcttt
     6421 actaaagctt ctgtagcagc tttgaaaaag tatccagaag ttaatgcaga aatcgacggc
     6481 gacgacatga ttacgaaaca atattatgat attggtgtag ctgtttctac agatgatgga
     6541 ttattagtac catttgtaag agattgtgat aaaaagaatt ttgcagaaat cgaagcagaa
     6601 attgctaatt tagcagttaa agcacgagag aaaaaacttg gcttagatga tatggttaat
     6661 ggttcattta cgattacaaa tggcggtatt tttggatcaa tgatgagtac gccaattatc
     6721 aatggtaatc aagctgcaat cttaggcatg cattcaatta ttacaagacc aattgcgatt
     6781 gatcaagata caatcgaaaa tcgtccaatg atgtatattg cattaagcta tgatcataga
     6841 attattgacg gtaaagaagc agttggattc ttaaaaacaa ttaaagaatt aattgaaaac
     6901 ccagaagact tattattaga atcttaatcc acaacacaaa atagtttact attcctaaaa
     6961 gcgggattaa atcaataaca aacagcagta ag
//
LOCUS       DAMZKC010000126         6932 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.127, whole genome shotgun sequence.
ACCESSION   DAMZKC010000126 DAMZKC010000000
VERSION     DAMZKC010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6932
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.127"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            32..565
                     /locus_tag="POB55_002010"
                     /pseudo
     CDS             32..565
                     /locus_tag="POB55_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(678..2168)
                     /locus_tag="POB55_002011"
     CDS             complement(678..2168)
                     /locus_tag="POB55_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HDI6500966.1"
                     /translation="MDDKQHTSSSDDERAEIATSNQDQETNSSKRVHLKRWQFISILI
                     GTILITAVITVVAYIFINQKISGLNKTDQANLNKIENVYKILNSDYYKKQDSDKLSKA
                     AIDGMVKELKDPYSEYLTKEQTKSFNEGVSGDFVGIGAEMQKKNDQIMVTSPMKGSPA
                     ERAGIRPKDVITKVNGKSIKGKALDEVVKDVRGKENTEVTLTVQRGSEEKDVKIKREK
                     IHVKSVEYKKKGKVGVITINKFQNDTSGELKDAVLKAHKDGLKKIVLDLRNNPGGLLD
                     EAVKMANIFIDKGKTVVKLEKGKDTEAIQTSNDALKEAKDMDISILVNEGSASASEVF
                     TGALKDYNKAKVYGSKTFGKGVVQTTREFKDGSLLKYTEMKWLTPDGHYIHGKGIKPD
                     VTIDTPKYQSLNVIPNTKTFKVGDDDKNIKTIKIGLSALGYKVDNETTQFDQALENQV
                     KAFQQANKLEVTGEFNKETNNKFTELLVEKANKHDDVLDKLINILK"
     gene            complement(2360..2581)
                     /locus_tag="POB55_002012"
     CDS             complement(2360..2581)
                     /locus_tag="POB55_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HDI6500967.1"
                     /translation="MKNYSFYQFVMTVRGRHDDKGRLAEEIFDDLAFPKHDDDFNILS
                     DYIETHGDFTLPMSVFDDLYEEYTEWLKF"
     gene            complement(2581..3081)
                     /locus_tag="POB55_002013"
     CDS             complement(2581..3081)
                     /locus_tag="POB55_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDI6500968.1"
                     /translation="MFKKLFGKGKEVQKDIAIYTPLTGEFVKIEDIPDPVFAQKMMGE
                     GFGINPTEGEVVSPIAGRVDNVFPTKHAIGLKADNGLELLVHIGLDTVQLDGEGFEVL
                     VSSGDEVNVGDPLVRFNLEYINNNAKSVISPIIITNTDQAASINIYDENAVIKGETKV
                     IDVTMN"
     gene            complement(3093..3521)
                     /gene="msrB"
                     /locus_tag="POB55_002014"
     CDS             complement(3093..3521)
                     /gene="msrB"
                     /locus_tag="POB55_002014"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HDI6500969.1"
                     /translation="MLKKDKSELTDIEYIVTQENGTEPPFMNEYWNHFAKGIYVDKIS
                     GKPLFTSEEKFHSECGWPSFSKALDDDEIIELVDKSFGMLRTEVRSEESNSHLGHVFN
                     DGPKESGGLRYCINSAAIQFIPYEKLEELGYGDLISHFDK"
     gene            complement(3514..4047)
                     /gene="msrA"
                     /locus_tag="POB55_002015"
     CDS             complement(3514..4047)
                     /gene="msrA"
                     /locus_tag="POB55_002015"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDI6500970.1"
                     /translation="MTKEYATLAGGCFWCMVKPFTSYPGIKSVVSGYSGGHVDNPTYE
                     QVCTNQTGHVEAVQITFDPEVTSFENILDIYFKTFDPTDDQGQFFDRGESYQPVIFYH
                     DEHQKKAAEFKKQQLNEQGIFKKPVITPIKPYKNFYPAEDYHQDYYKKNPVHYYQYQR
                     GSGRKAFIESHWGNQNA"
     gene            complement(4133..4972)
                     /gene="fakB2"
                     /locus_tag="POB55_002016"
     CDS             complement(4133..4972)
                     /gene="fakB2"
                     /locus_tag="POB55_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase binding subunit FakB2"
                     /protein_id="HDI6500971.1"
                     /translation="MTKQIIVTDSTSDLSKEYLEANNIHVIPLSLTIEGASYVDQVDI
                     TSEEFINHIENDEDVKTSQPAIGEFISAYEELGKDGSEIISIHLSSGLSGTYNTAYQA
                     SQMVDANVTVIDSKSISFGLGYQIQHLVELVKEGVSTSEIVKKLNHLRENIKLFVVIG
                     QLNQLIKGGRISKTKGLIGNLMKIKPIGTLDDGRLELVHNARTQNSSIQYLKKEIAEF
                     IGDHEIKSIGVAHANVIEYVDKLKKVFNEAFHVNNYDINVTTPVISAHTGQGAIGLVV
                     LKK"
     gene            complement(4987..5466)
                     /locus_tag="POB55_002017"
     CDS             complement(4987..5466)
                     /locus_tag="POB55_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDI6500972.1"
                     /translation="MTLSILVAHDLQRVIGFENQLPWHLPNDLKHVKKLSTGHTLVMG
                     RKTFESIGKPLPNRRNVVLTSDTSFNVVGVDVIHSIEDIYQLPGHVFIFGGQILFEEM
                     IDKVDDMYITVIEGKFRGDTFFPPYTFEDWEVASSVEGKLDEKNTIPHTFLHLIRKK"
     gene            complement(5666..6622)
                     /locus_tag="POB55_002018"
     CDS             complement(5666..6622)
                     /locus_tag="POB55_002018"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDI6500973.1"
                     /translation="MLNSFDAAYHSLCEEVLEIGNTRNDRTNTGTISKFGHQLRFDLS
                     KGFPLLTTKKVSFKLVATELLWFIKGDTNIQYLLKYNNNIWNEWAFENYIKSDEYNGP
                     DMTDFGHRALSDPEFNEQYKEQMKQFKQRILEDDTFAKQFGDLGNVYGKQWRDWVDKD
                     GNHFDQLKTVIEQIKHNPDSRRHIVSAWNPTEIDTMALPPCHTMFQFYVQDGKLSCQL
                     YQRSADIFLGVPFNIASYALLTHLIAKECGLEVGEFVHTFGDAHIYSNHIDAIQTQLA
                     RESFNPPTLKINSDKSIFDINYEDLEIVDYESHPAIKAPIAV"
BASE COUNT         2161 a         1214 c          967 g         2590 t
ORIGIN      
        1 tgtaatttta cttttaaata ctttaaaaaa aataagacac tttgcccaac ttacactacc
       61 aatagaaact tctgttagaa ttcctcaaaa tgatatttca cgatatgtta atgaaattgt
      121 tgaaacgata cctgatagcg aattcgatga attcagacat catcgtggcg caacatccta
      181 tcatccaaaa atgatgttaa aaattatctt atatgcatac actcaatctg tattttctgg
      241 tcgtagaata gagaaattac ttcatgacag tattcgaatg atgtggttag ctcaaaatca
      301 aacaccttct tataaaacta ttaatcgttt tagagtaaat cctaatactg atgcattaat
      361 tgaatcttta tttatccagt ttcatagtca atgtttaaag caaaatctta ttgatcataa
      421 ttcaattttt attgatggta caaaagtaga agctaatgcc aataggtata catttgtgtg
      481 gaagaaaagt attccaaatc acgaatcgaa attgaacgaa aattcaaaaa cactatatcg
      541 tgacttagtt gaagaaaagc tgatttctat caaataatta atagaaatca gcttttttac
      601 attgcctaag aacttaatgt tccagcctga gcgtgtcatt aacataataa taatacaatt
      661 ttagcagtgt gtatctctta ttttaaaata ttaatcaatt tatcgagaac atcatcatgt
      721 ttattagctt tttcaactaa taactcagta aatttattat tcgtttcttt attaaattca
      781 ccagttacct cgagtttatt cgcttgttgg aaagctttaa cttgattttc taaagcttga
      841 tcaaattgcg ttgtttcatt atcaacttta taacctaaag ctgacaaacc aattttaata
      901 gttttaatat ttttatcatc gtctccaact ttaaatgttt tcgtattagg aatgacattt
      961 aaagattgat atttaggtgt gtcaatagta acgtctggtt taatgccttt accgtgaata
     1021 taatgaccat ctggcgttaa ccatttcatt tcagtatatt ttaacaatga accatcctta
     1081 aactctcttg tagtttgtac gacacctttg ccgaatgttt ttgacccata aactttagct
     1141 ttattatagt cttttagcgc accagtaaac acttcagaag cgctagctga accttcattc
     1201 actaagatgg atatatccat gtctttcgct tcttttaacg catcattaga agtttgaatt
     1261 gcttcagtat ctttaccttt ttctagttta acaacagttt ttcctttatc gataaaaata
     1321 tttgccattt taacagcttc atctagtagt ccacctggat tatttcttaa atctaaaaca
     1381 atctttttca aaccatcttt gtgagctttt agaactgcat ctttcaattc acctgatgta
     1441 tcattctgga atttattaat agtaataact ccaactttac cttttttctt atactcaaca
     1501 cttttaacat gaattttttc acgtttaatc ttaacgtctt tttcttcact acctcgttga
     1561 acagttaaag tgacttcagt gttttcttta ccacgaacat ctttgacaac ttcatctaat
     1621 gcttttcctt taattgattt tccatttact ttagtaatga catctttagg acgaatgcca
     1681 gcacgttctg ctggagatcc cttcatagga ctagtaacca taatttgatc atttttcttt
     1741 tgcatttctg caccaatacc tacaaaatca cctgaaacac cttcattaaa ggatttcgtt
     1801 tgttcttttg ttaaatattc agaataagga tcttttaatt ctttgaccat gccatcaatt
     1861 gcagctttac ttaacttgtc agagtcctgt tttttgtaat aatcactatt taagatttta
     1921 tacacatttt caattttatt taagtttgct tgatcagttt tgtttaaacc acttattttt
     1981 tgatttataa aaatatatgc aacaactgtg atgacagctg ttattaggat tgtaccaatt
     2041 aatattgata tgaattgcca acgttttaag tgaacgcgtt tcgatgaatt agtttcttgg
     2101 tcttgattgc ttgttgcaat ttcagcgcgt tcatcatcgg atgaagatgt gtgttgctta
     2161 tcatccattg tatcacttcc ttattatgta ttatgtactt ttaaattctg tgtagttaat
     2221 catatcaatt tatgcaagat agcgcaccat atttcactat aattgattaa tttttagtca
     2281 caacttagtt tattttaaat taaaaccagg ataacgaatt gcatcaccct ggttctaact
     2341 attattatta aaaattatat taaaatttta gccattccgt atattcttca tataaatcat
     2401 caaatacaga cattggcaat gtgaaatcac catgtgtctc aatataatca gacagtatgt
     2461 taaaatcatc atcgtgtttt gggaaagcaa gatcgtcaaa tatctcttct gctagacgac
     2521 ctttatcgtc gtgtcgacca cgaactgtca tgacaaattg ataaaacgaa tagtttttca
     2581 ttagttcatt gtcacatcaa tcacttttgt ttcaccttta atcacagcat tttcatcata
     2641 aatattaatt gaagctgctt gatcagtgtt agtaattata attggtgaaa ttacagattt
     2701 agcgttatta ttaatatatt caaggttgaa tcttactaat ggatctccga cgttaacttc
     2761 gtcaccacta gacactaaca cttcaaatcc ttcaccgtct aattgaactg tgtctaaacc
     2821 gatatgaact aataattcta atccgttatc tgcttttaac ccaatcgcat gcttagttgg
     2881 aaagacattg tcaacacgtc ctgcaattgg agacacaact tctccttcag ttggattaat
     2941 accaaaacct tcgcccatca ttttttgtgc gaatacagga tctggaatat cttcaatttt
     3001 cacgaattct ccagttaatg gtgtataaat tgcgatatct ttctgaactt ctttgccttt
     3061 tccgaataat tttttaaaca tactttccac tcctacttat caaaatgtga tattaaatcg
     3121 ccataaccca attcttctaa cttttcatat ggaataaatt gaattgcagc ggaattgata
     3181 cagtatctta agccgccact ttctttaggt ccatcattaa agacatgtcc taaatgacta
     3241 tttgattctt ctgaacgcac ttcagttctc aacataccaa atgatttgtc gactaattct
     3301 ataatttcat cgtcatcaag cgctttggaa aagctaggcc atccacattc agaatgaaac
     3361 ttttcttcag atgtaaataa aggtttacca gaaattttat ctacataaat tcctttagca
     3421 aaatgattcc aatattcatt cataaatggt ggttcagtgc cgttttcttg tgtaacaata
     3481 tattctatat ctgttagttc acttttatct tttttaagca ttttgattcc cccaatgtga
     3541 ttctataaac gcttttctac ctgaaccacg ttgatattgg taataatgta ccgggttctt
     3601 tttgtaataa tcttgatggt agtcttcagc tggatagaaa tttttatatg gtttaatagg
     3661 tgtaatcact ggtttcttga aaataccttg ttcatttaat tgttgctttt taaactcagc
     3721 agcctttttc tgatgttcat catgatagaa aatgactggt tgatagcttt cgcctctatc
     3781 gaaaaattgc ccttgatcat cagttgggtc aaatgttttg aaatatatgt ctaatatatt
     3841 ttcaaaggaa gtaacctctg gatcaaacgt aatttgtact gcttcgacat ggccggtttg
     3901 attcgtacat acctgttcat aagttgggtt gtcaacatga ccgccactat aaccagatac
     3961 gactgacttg atgcctggat atgatgtaaa tggtttaacc atgcaccaga aacatcctcc
     4021 tgctaatgtt gcatattctt ttgtcatatg aattacctcc tctatctatc taattataaa
     4081 tttagtctat aaaggattga aatggaagta attaacactg aaaagattaa atttacttct
     4141 taaggactac gaggccaatc gcaccttgac cagtatgtgc agaaataact ggtgtagtta
     4201 catttatatc gtaattattc acatgaaaag cttcattaaa aactttcttc aatttatcaa
     4261 catattcaat gacgttagca tgtgcgacac caatggattt gatttcatga tctcctataa
     4321 attcagcaat ttcctttttc aagtattgga tactagaatt ttgagttctc gcattgtgca
     4381 caagctctaa gcgaccatca tctagtgtac caattggttt aattttcata agattaccaa
     4441 tcaaaccttt tgttttacta attctgccac ctttaattaa ttgattcaat tgccctataa
     4501 ctacaaataa tttaatgttt tctcttaaat gatttaactt tttaactatt tcagaagttg
     4561 agacaccttc ttttacaagc tctactaggt gttgtatttg ataccctaaa ccaaaagaaa
     4621 tagattttga atcaataaca gttacattag catctaccat ttgacttgct tggtaagcag
     4681 tgttatatgt accacttaat cctgaagaaa gatgaatact tatgatttca gagccatctt
     4741 ttcctagttc ttcataagca gatataaatt cacctatggc tggctgactt gtctttacat
     4801 cttcatcatt ttcaatatga ttaataaatt cttctgatgt aatatctact tggtcaacgt
     4861 atgaagctcc ttcaatagtt aaacttaaag gaattacatg aatgttgttt gcttctaagt
     4921 attctttaga taaatcggat gttgagtctg ttactataat ctgttttgtc atggtcgttt
     4981 tcccccttat tttttacgaa ttaaatgtag aaaggtatgt ggaattgtat ttttctcatc
     5041 tagtttacct tcaactgaag aggcaacttc ccagtcttca aatgtataag gtggaaagaa
     5101 cgtatcacca cggaatttac cttcaataac agtaatatac atgtcgtcca ctttatcaat
     5161 catttcttca aataatattt gccctccaaa tatgaaaaca tggcccggta gttggtaaat
     5221 atcttcaata gagtgaatta catcaacgcc cactacgttg aaacttgtat ctgaagtaag
     5281 tacaacattt cgacgattcg gtagtggttt accaatcgat tcaaatgtct tacgacccat
     5341 tactaaagta tgacctgttg ataatttttt aacatgtttc aaatcatttg gtaggtgcca
     5401 aggtaattga ttttcaaaac caattactcg ttgcaagtca tgtgcaacta gaatggataa
     5461 agtcataatt atcctccttc ttctatcatt tcatttttta ttactaagtt atctttaatt
     5521 taacacaatt tttatcataa agtgtgatag aaataatgat tttgcataat ttatgaaaac
     5581 gtttaacaca aaaaagtact tttttgcact tgaaaatact atgatgtcat tttgatgtct
     5641 atatggttag ctaactatgc aatgactaca ctgctattgg agcttttatt gctggatgtg
     5701 attcatagtc aacaatttcc aaatcttcat aatttatgtc gaaaatagac ttgtcactgt
     5761 taatttttaa tgttggagga ttgaagcttt cacgtgctaa ttgtgtttga atcgcatcaa
     5821 tatgatttga ataaatatgt gcatctccaa atgtatgcac aaattctcct acttcaagtc
     5881 cacattcttt ggcaataagg tgtgtcaata aagcgtagct tgcgatatta aatggcacac
     5941 ctaaaaagat atctgcgcta cgttggtata actggcaact taacttacca tcttggacat
     6001 aaaactggaa catggtatga caaggcggaa gtgccattgt atcaatttct gttggattcc
     6061 atgcagatac gatgtgtcgt cttgaatctg gattatgctt aatttgttca attactgttt
     6121 taagttgatc aaaatgatta ccatctttat caacccaatc tcgccattgt ttaccataaa
     6181 catttcctaa atcaccgaat tgcttcgcaa aagtatcatc ttcaagaata cgttgcttaa
     6241 attgtttcat ttgttcttta tattgttcgt taaattcagg atcactcaat gcacgatgcc
     6301 cgaaatctgt catatctgga ccgttatact cgtctgattt tatataattt tcaaaagccc
     6361 attcgttcca aatattatta ttatatttta ataagtattg gatgtttgta tctcctttaa
     6421 tgaaccataa taattcggtt gctactaatt taaaagaaac tttctttgtc gttaatagtg
     6481 gaaatccttt agataagtca aagcgaagtt gatgaccaaa tttcgaaatc gtacctgtat
     6541 ttgtgcgatc atttcgtgta tttcctattt ctaaaacttc ttcacaaaga ctgtgatatg
     6601 ctgcatcaaa tgaattcaac atatgcgata acacctcatt ttcattattt atagtatgta
     6661 tatttagttt gatataactt aattttatgt agcattttgt tatcactcat tttaggaata
     6721 tgatattaat atcatgaatt ccgttacttt atttataaaa tgctgattaa gtacctacca
     6781 catcgtaacg tgatatatgt ttccaattgg taattgttta cccaaatcta taactttaat
     6841 gctaaaaaat ttaaaaaaag aggttaacac atgatttgaa tattatgttt gatgtcctat
     6901 taaaacagtt aaatttctag aaaatatagt tg
//
LOCUS       DAMZKC010000127         6844 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.128, whole genome shotgun sequence.
ACCESSION   DAMZKC010000127 DAMZKC010000000
VERSION     DAMZKC010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6844
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.128"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(82..642)
                     /gene="icaR"
                     /locus_tag="POB55_002019"
     CDS             complement(82..642)
                     /gene="icaR"
                     /locus_tag="POB55_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ica operon transcriptional regulator IcaR"
                     /protein_id="HDI6500974.1"
                     /translation="MKDKIIDNAITLFSEKGYDGTTLDDIAKSVNIKKASLYYHFDSK
                     KSIYEQSVKCCFDYLNNIIMMNQNKSNYSIDALYQFLFEFIFDIEERYIRMYVQLSNT
                     PEEFSGNIYGQIQDLNQSLSKEIAKFYDESKIKMTKEDFQNLILLFLESWYLKASFSQ
                     KFGAVEESKSQFKDEVYSLLNIFLKK"
     gene            806..2044
                     /gene="pgaC"
                     /locus_tag="POB55_002020"
     CDS             806..2044
                     /gene="pgaC"
                     /locus_tag="POB55_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine synthase"
                     /protein_id="HDI6500975.1"
                     /translation="MQFFNFLLFYPVFMSIYWIVGSIYFYFTREIRYSLNKKPDINVD
                     ELEGITFLLACYNESDTIEDTLSNVLALKYEKKEIIIINDGSSDNTAELIYKIKENND
                     FIFVDLQENRGKANALNQGIKQASYDYVMCLDADTIVDQDAPYYMIENFKHDPKLGAV
                     TGNPRIRNKSSILGKIQTIEYASLIGCIKRSQTLAGAVNTISGVFTLFKKSAVVDVGY
                     WDTDMITEDIAVSWKLHLRGYRIKYEPLAMCWMLVPETLGGLWKQRVRWAQGGHEVLL
                     RDFFSTMKTKRFPLYILMFEQIISILWVYIVLLYLGYLFITANFLDYTFMTYSFSIFL
                     LSSFTMTFINVIQFTVALFIDSRYEKKNMAGLIFVSWYPTVYWIINAAVVLVAFPKAL
                     KRKKGGYATWSSPDRGNTQR"
     gene            2008..2313
                     /gene="icaD"
                     /locus_tag="POB55_002021"
     CDS             2008..2313
                     /gene="icaD"
                     /locus_tag="POB55_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular adhesion protein D"
                     /protein_id="HDI6500976.1"
                     /translation="MVKPRQREYPTLKSSLNIVRETALIAISCVFWIYCLVVLLVYIG
                     TIFEIHDESINTIRVALNIENTEILDIFETMGIFAIIIFVFFTISILIQKWQRGRES"
     gene            2310..3182
                     /gene="icaB"
                     /locus_tag="POB55_002022"
     CDS             2310..3182
                     /gene="icaB"
                     /locus_tag="POB55_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intercellular adhesin biosynthesis
                     polysaccharide N-deacetylase"
                     /protein_id="HDI6500977.1"
                     /translation="MKYRKFIILVLSILIILPVSTLDGHHIANADDDSPKKLKYKENS
                     ALALNYHRVRKANFLNNFIYFFSSSKEIKNYSVSQSQFESQIKWLKSHDAKFLTLKEF
                     LYYKKKGKFPKRSVWINFDDMDETIYENAYPILKKYKIPATGFIITGHVGEENFHNLD
                     MISKKELKEMYKTGLWEFETHTHDLHNLSKNNKSKLIKASEATIIKDLNKSEKYLTKN
                     FKKSQKTIAYPYGLMNDDKLPVIKKAGLKYGFSLEEKAVTPNSNDYYIPRILISDDAF
                     EHLIKRWDGFHEKD"
     gene            3169..4221
                     /gene="icaC"
                     /locus_tag="POB55_002023"
     CDS             3169..4221
                     /gene="icaC"
                     /locus_tag="POB55_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide intercellular adhesin
                     biosynthesis/export protein IcaC"
                     /protein_id="HDI6500978.1"
                     /translation="MKKIRLELVYLRAIICAIIIITHLLTQITLKHENMEGGSLVLQF
                     YIRNIVIFGTPCFIILSQLLTTLNYQKVTYRYLTTRVKYILIPYILMGLFYSYSESLL
                     TDSSFNKQFIENVLLGQWYGYFIVVIMQFFILSYIIFKINYNLFNSKILLLLSFILQQ
                     SFLYYFTNNTAFHDTVLHYYPLSENTIIFGWIFYFFLGAYMGYNYERVLNFLERYLVI
                     MIVLAVATYFVFIALANGDYWNVTSFSYSLTPYNSIMFIVILGICTHFKTMLFNTIQM
                     ISAFSFFIYLLHPIILDSLFAYTNIFEDNTMVFLAISLLFILGLCIGVGMILREFYIF
                     RFIIGKQPYKLNINAY"
     gene            complement(4667..6709)
                     /gene="lip1"
                     /locus_tag="POB55_002024"
     CDS             complement(4667..6709)
                     /gene="lip1"
                     /locus_tag="POB55_002024"
                     /EC_number="3.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK domain-containing triacylglycerol lipase
                     Lip1"
                     /protein_id="HDI6500979.1"
                     /translation="MKSQNKYSIRKFSVGASSILIATLLFLSGGQAQAAEKQVNMGNS
                     QEDTVTAQSIGDQQTRENANYQRENGVDEQQHTENLTKNLHNDKTISEENHRKTDDLN
                     KDQLKDDKKSSLNNKNIQRDTTKNNNANPSDVNQGLEQAINDGKQSKVASQQQSKEAD
                     NSQDSNANNNLPSQSRIKEAPSLNKLDQTSQREIVNETEIEKVQPQQNNQANDKITNY
                     NFNNEQEVKPQKDEKTLSVSDLKNNQKSPVEPTKDNDKKNGLNLLKSSAVATLPNKGT
                     KELTAKAKDDQTNKVAKQGQYKNQDPIVLVHGFNGFTDDINPSVLAHYWGGNKMNIRQ
                     DLEENGYKAYEASISAFGSNYDRAVELYYYIKGGRVDYGAAHAAKYGHERYGKTYEGI
                     YKDWKPGQKVHLVGHSMGGQTIRQLEELLRNGNREEIEYQKKHGGEISPLFKGNHDNM
                     ISSITTLGTPHNGTHASDLAGNEALVRQIVFDIGKMFGNKNSRVDFGLAQWGLKQKPN
                     ESYIDYVKRVKQSNLWKSKDNGFYDLTREGATDLNRKTSLNPNIVYKTYTGEATHKAL
                     NSDRQKADLNMFFPFVITGNLIGKATEKEWRENDGLVSVISSQHPFNQAYTKATDKIQ
                     KGIWQVTPTKHDWDHVDFVGQDSSDTVRTREELQDFWHHLADDLVKTEKLTDTKQA"
BASE COUNT         2133 a         1120 c         1038 g         2553 t
ORIGIN      
        1 caactttata tagatttaaa aaaataatct catgtctttt tttacaaaag taagttaatt
       61 attacaaact agtaacaaaa attatttctt caaaaatata tttagtagcg aatacacttc
      121 atctttgaat tgacttttac tttcttccac tgctccaaat ttttgcgaaa aggatgcttt
      181 caaataccaa ctttcaagaa acagcaatat taaattctga aagtcttctt ttgtcatctt
      241 tatctttgat tcatcataga attttgctat ctctttactt aatgattgat ttaaatcttg
      301 tatttgtccg taaatatttc cagaaaattc ctcaggcgta ttagataatt gaacgtacat
      361 tctaatatac ctttcttcga tgtcgaaaat aaactcaaat aagaattgat ataaagcatc
      421 aattgaatag ttcgatttat tttgattcat cataataata ttattaaggt aatcaaaaca
      481 acatttaaca ctttgttcgt aaatactttt tttcgagtca aaatggtaat ataaactcgc
      541 tttctttata tttacacttt tagctatatc atcaagtgtt gtaccgtcat accccttctc
      601 tgaaaataag gttattgcgt tatcaataat cttatccttc aatttttata accccctact
      661 gaaaattaat cacactatgt tacaggaaaa ttaagttgca attacaaata tttccgttta
      721 attataacaa caatctattg caaattaaaa tactatcaat taccatatgg cttacaacct
      781 aactaacgaa aggtaggtaa agaaattgca attttttaac tttttgcttt tttatcctgt
      841 atttatgtct atttactgga ttgtcggttc aatttatttc tattttacca gagaaattag
      901 atattcattg aacaagaagc ctgacataaa tgtggatgaa ttagaaggca ttacattttt
      961 acttgcctgt tataacgaaa gtgacacgat tgaagatacg ttgtctaatg ttcttgcact
     1021 caaatacgag aagaaagaaa ttattatcat taatgatgga agttcagata atacagcaga
     1081 actcatctat aaaatcaaag aaaataatga ctttattttc gtcgatttac aagaaaacag
     1141 aggtaaagcc aacgcactca atcaaggcat taaacaggct tcatatgatt atgtaatgtg
     1201 cttggatgca gatactatcg ttgatcaaga tgcaccatat tatatgattg agaatttcaa
     1261 acatgatcca aaacttggtg cagttacagg taatcctaga attcgaaata agagttctat
     1321 tttaggtaaa attcaaacga tagaatatgc aagtttaatt ggctgtatta agcgaagtca
     1381 gacacttgct ggcgcagtca atactatttc gggtgtcttc actctattta aaaaaagtgc
     1441 agttgtcgac gttggctact gggatactga tatgattacc gaagatattg cagtttcttg
     1501 gaaattgcat ttacgtggat atcgtattaa gtatgaaccg cttgccatgt gttggatgtt
     1561 ggttccagaa acattgggag gtctttggaa gcaacgcgtg agatgggctc aagggggaca
     1621 cgaagtatta ctacgagact tttttagcac aatgaaaacg aaaaggtttc ctttatatat
     1681 tttgatgttt gagcaaatca tctcgatttt atgggtatat atagtgcttc tatatttagg
     1741 ctatttgttc ataacagcaa acttcttaga ctatacattt atgacatata gtttttcaat
     1801 atttctacta tcatcattta ctatgacttt tataaacgtt attcaattta cagtcgcact
     1861 ctttattgat agtcgctacg agaaaaagaa tatggctgga ctcatatttg taagttggta
     1921 tccgacagta tactggatta ttaacgcagc agtagttctt gtcgcatttc caaaagcatt
     1981 aaaacgtaag aaaggtggtt acgcaacatg gtcaagccca gacagaggga atacccaacg
     2041 ctaaaatcat cgctaaatat tgtaagagaa acagcactta tcgctatatc ttgtgtcttt
     2101 tggatatatt gtttagttgt tctactcgtt tatattggta ctatatttga aattcatgac
     2161 gaaagtatca atacaatacg tgttgcttta aacattgaaa atactgaaat tttagatata
     2221 tttgaaacta tgggcatttt cgcgattatc atttttgtat tttttacaat tagcatattg
     2281 attcaaaaat ggcagagagg aagagaatcg tgaagtatag aaaatttata attttagtgt
     2341 tgagtatctt gatcatattg cctgtaagca cactggatgg tcatcatatt gcaaatgcag
     2401 atgacgattc acctaaaaaa ctgaaatata aagaaaatag tgctctggca ttaaattatc
     2461 accgtgtaag aaaagcgaat tttctgaata attttattta cttcttttct agtagtaaag
     2521 aaattaaaaa ttatagtgtt agtcaatcac aatttgaatc tcaaataaaa tggctaaaat
     2581 cacatgatgc taaattttta accttgaaag aatttttata ttacaagaaa aaaggtaagt
     2641 ttccaaaacg aagtgtatgg attaactttg atgatatgga tgaaactatt tatgaaaatg
     2701 cttatccaat cttaaaaaaa tataaaatac cggcaactgg gtttattatc acaggtcatg
     2761 ttggggaaga aaactttcac aacctcgata tgattagtaa aaaagaacta aaagaaatgt
     2821 ataaaactgg gttatgggaa tttgaaacac atacccacga tttgcataac ttatctaaaa
     2881 ataataagtc aaaattaata aaagcttctg aagctacaat cataaaagat ttaaacaaaa
     2941 gtgaaaaata tctaactaaa aactttaaaa agtcgcagaa aactatagcc tatccttatg
     3001 gcttgatgaa tgacgataaa ttaccggtaa tcaaaaaagc tgggttaaaa tacggttttt
     3061 cattagagga aaaagcagtc actccgaact ccaatgatta ttacatccct agaatattaa
     3121 ttagtgatga tgcttttgag catttaatta agagatggga cggattccat gaaaaagatt
     3181 agacttgaac tcgtatattt acgtgctatt atatgtgcaa ttattattat cacacattta
     3241 cttacacaaa ttactttaaa acatgaaaat atggagggtg gatccttagt gttacaattt
     3301 tacattcgta atattgtgat ttttggtaca ccttgcttta ttatcttgtc acagttactg
     3361 acaaccttga attaccaaaa agtcacctat agatacttaa ctacacgcgt aaaatatata
     3421 cttattcctt acatattaat gggattgttt tacagttata gtgaatcatt attaacagat
     3481 tcaagtttca ataaacaatt cattgaaaat gtcctattag gtcaatggta tggctatttt
     3541 atcgttgtta tcatgcaatt ctttattttg agttatatca tttttaaaat taactataac
     3601 ctattcaaca gtaaaatatt attattgtta tcttttattt tacagcaatc atttttatat
     3661 tactttacga acaacacagc gtttcacgat accgtgctac actattatcc attaagtgaa
     3721 aatactataa tattcggatg gattttttat ttcttcttag gtgcatatat gggttataac
     3781 tacgaacgtg tattaaattt cttagaacgt tatttagtta ttatgattgt attagctgta
     3841 gctacttatt ttgtgtttat tgcgttagca aatggagact attggaacgt taccagcttt
     3901 tcatattcat taacaccata taatagtatt atgtttattg ttatcttggg tatttgcacg
     3961 cattttaaaa caatgttatt taatacgatt caaatgatta gtgctttctc attctttatt
     4021 tatttattac atccaatcat tctagactca ttgtttgcat atacaaatat atttgaggat
     4081 aatacaatgg tctttctagc gatatcacta ctattcattt taggattatg tataggtgtc
     4141 ggcatgatat tgcgtgaatt ctatatcttt aggtttatta ttggaaaaca accatataaa
     4201 ttgaacatta atgcttatta attattaagc tatgttaaaa acacgcggtg ggcgaaatca
     4261 gtttgaattg actgacttcg ttttaccgcg tgtttaatat tgttatacat atattctaat
     4321 tgcacattta aacttcgtaa atgccaatgg gagtgggaca gaaatgatat tttcgcaaaa
     4381 ttatttcgtc gtcccacccc aacttgcaca ttattgtaac ctgactttcc gccagcttct
     4441 atgttggggc cccgccaact tgcacattat tgtaagctga ctttccgcca gcttctttgt
     4501 tggggccccg ccaacttgca ttgtttgtag aatttctttt cgaaattctt tatgttgggg
     4561 cctcgccaat gttttacttg aataattctt ttagaattct aaataatgat ccgattaatt
     4621 gaaagaagtc tgcagtcatt attaattcct ccctttactt tataaattat gcttgcttag
     4681 tatcagtcag cttttcagtt ttcactaaat cgtctgctaa atgatgccaa aaatcttgta
     4741 attcttctct tgtgcgcact gtatcagaac tgtcttgtcc tacaaagtca acatgatccc
     4801 aatcatgttt tgtaggcgtc acttgccaaa tgcctttttg aattttatct gtcgcttttg
     4861 tataagcttg attaaatgga tgttgagaag aaataacgga tactaaacca tcgttttctc
     4921 gccattcttt ttcagtagct ttaccgatta agttaccagt aatcacaaat gggaaaaaca
     4981 tatttaagtc tgctttttgt ctatcgctat ttaatgcttt gtgcgttgct tcaccagtgt
     5041 atgttttata cacaatgtta gggttcaacg acgttttacg atttaaatct gttgcaccct
     5101 cacgcgtcag atcgtaaaat ccattatctt ttgatttcca taaattagat tgtttaacgc
     5161 gtttgacata atcaatatat gattcatttg gcttctgttt tagaccccat tgagccaacc
     5221 cgaagtctac tcttgaattt ttattaccaa acattttacc gatatcaaat acgatttgtc
     5281 tcactaaagc ttcattacca gctaaatctg atgcgtgtgt accattatgt ggtgttccta
     5341 aagtagtaat tgatgaaatc atattgtcat gattaccttt gaatagtgga gaaatttcgc
     5401 caccatgttt cttttgatac tctatttctt cacgattacc attacgcagt aattcttcta
     5461 gttgacgtat cgtttgaccg cccatactat gtccaactag gtgtaccttc tgtcctggtt
     5521 tccagtcttt gtaaattcct tcgtatgttt ttccataacg ttcatgtcca tattttgctg
     5581 catgtgctgc accataatct acacgaccgc ctttgatata ataataaagt tcaactgcgc
     5641 ggtcatagtt acttccaaaa gcacttatac ttgcttcata agctttgtaa ccattttctt
     5701 ctaaatcttg gcgaatgttc attttattac cgccccaata atgagctaac actgaaggat
     5761 taatatcatc tgtaaaccca ttgaaaccat gcactaaaac gataggatcc tgatttttat
     5821 actgcccttg tttggcaact ttattcgttt gatcatcttt cgcttttgca gtaagttcct
     5881 ttgtcccttt gtttggtaac gttgctactg cactactttt taataaattt aatccatttt
     5941 tcttgtcatt gtcctttgtt ggttctactg gtgatttttg attgtttttt aaatctgaaa
     6001 ctgatagtgt tttttcgtct ttttgaggtt tcacttcttg ttcattgtta aaattgtagt
     6061 tagtaatttt atcattcgct tgattatttt gttgtggttg tactttctct atttctgtct
     6121 cattaacaat ttctcgttga cttgtttgat ctaacttatt taatgatggt gcttccttta
     6181 ttcgactttg tgaaggtaga ttgttattag cgtttgaatc ttgactatta tctgcctctt
     6241 ttgactgttg ctgtgacgcc actttacttt gtttaccatc attaatagcc tgttctaacc
     6301 cttgatttac atcgctagga ttagcattgt tattttttgt tgtatcacgt tgaatatttt
     6361 tattattaag cgatgatttt ttatcatcct ttagttgatc tttattcaaa tcatctgttt
     6421 tacgatgatt ttcttctgat attgttttat cattatgcaa gttcttagtt aaattttcag
     6481 tatgttgctg ttcgtcaaca ccgttttcac gttgataatt agcattttcc ctagtttgtt
     6541 gatccccaat agattgtgct gtaactgtat cctcctgtga atttcccata ttcacttgct
     6601 tctcagctgc ttgtgcttgt ccaccactta aaaatagtaa tgtagctatt aaaatggaag
     6661 atgcacctac actaaattta cgaatactat acttattttg acttttcatc attgtcagca
     6721 cctctatttt tttattttag ataaacaaat tgataattat tcgaggaata aactcaaata
     6781 atttacttaa ccaagcactc ataatgacca tcctttctat tgtttaattt gacgagaaag
     6841 cgtt
//
LOCUS       DAMZKC010000128         6834 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.129, whole genome shotgun sequence.
ACCESSION   DAMZKC010000128 DAMZKC010000000
VERSION     DAMZKC010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6834
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.129"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            182..1165
                     /locus_tag="POB55_002025"
     CDS             182..1165
                     /locus_tag="POB55_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HDI6500980.1"
                     /translation="MKKWQFVGTTALGATLLLGACGGGNGGSGNSDLKGEAKGDGSST
                     VAPIVEKLNEKWAQDHSDAKISAGQAGTGAGFQKFIAGDIDFADASRPIKDEEKQKLQ
                     DKNIKYKEFKIAQDGVTVAVNKENDFVDELDKQQLKAIYSGKAKTWKDVNSKWPDKKI
                     NAVSPNSSHGTYDFFENEVMNKEDIKAEKNADTNAIVSSVTKNKEGIGYFGYNFYVQN
                     KDKLKEVKIKDENGKATEPTKKTIQDNSYALSRPLFIYVNEKALKDNKVMSEFIKFVL
                     EDKGKAAEEAGYVAAPEKTYKSQLDDLKAFIDKNQKSDDKKSDDKKSEDKK"
     gene            1356..2282
                     /gene="pstC"
                     /locus_tag="POB55_002026"
     CDS             1356..2282
                     /gene="pstC"
                     /locus_tag="POB55_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HDI6500981.1"
                     /translation="MTSSTNVKALIEKNNNKKGKHNDKIIPVILAAISAISILTTLGI
                     LITLLLETITFFTRIPITEFLFSTTWNPTGSDPKFGIWALIIGTLKITVIATIFAVPV
                     GLGAAIYLSEYASDRARRIIKPILEILAGIPTIVFGFFALTFVTPVLRSFIPGLGEFN
                     AISPGLVVGIMIVPLITSLSEDAMASVPNKIREGAYGLGATKLEVATKVVLPAATSGI
                     VASIVLAISRAIGETMIVSLAAGSSPTASLSLTSSIQTMTGYIVEIATGDATFGSNIY
                     YSIYAVGFTLFIFTLIMNLLSQWISKRFREEY"
     gene            2284..3201
                     /gene="pstA"
                     /locus_tag="POB55_002027"
     CDS             2284..3201
                     /gene="pstA"
                     /locus_tag="POB55_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDI6500982.1"
                     /translation="METTDNNRQSLVDQQLVQKHLSSRTVKNKVFKLIFLACTLLGLV
                     VLIALLTQTLIKGVSHLNLQFFTNFSSSTPSMAGVKGALIGSLWLMLSIIPLSIILGI
                     GTAIYLEEYAKNNKFTQFVKISISNLAGVPSVVFGILGYTLFVGGAGIEALKMGNSIL
                     AAALTMTLLILPIIIVSSQEAIRAVPNSVREASYGLGANKWQTIRRVVLPAALPGILT
                     GFILSLSRALGETAPLVLIGIPTILLATPRSILDQFSALPIQIFTWAKMPQEEFQNVA
                     SAGIIVLLVILILMNGVAIILRNKFSKKF"
     gene            3248..4099
                     /locus_tag="POB55_002028"
     CDS             3248..4099
                     /locus_tag="POB55_002028"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HDI6500983.1"
                     /translation="MAQTLAQTKQISQSHTFDVSQSHHKTPDDTNSHSVIYSTQNLDL
                     WYGENHALQNINLDIYENQITAIIGPSGCGKSTYIKTLNRMVELVPSVKTAGKILYRD
                     QDIFDQKYSKEQLRTNVGMVFQQPNPFPKSIYDNITYGPKIHGIKNKKVLDEIVEKSL
                     RGAAIWDELKDRLHTNAYSLSGGQQQRVCIARCLAIEPEVILMDEPTSALDPISTLRV
                     EELVQELKEKYTIIMVTHNMQQAARVSDKTAFFLNGYVNEYDDTDKIFSNPSNKKTED
                     YISGRFG"
     gene            4106..4747
                     /gene="phoU"
                     /locus_tag="POB55_002029"
     CDS             4106..4747
                     /gene="phoU"
                     /locus_tag="POB55_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HDI6500984.1"
                     /translation="MAIIRQRYQEQLDDLIKELRRLGANVYVSIENGIKSLSIDDRGF
                     ARQTVKNDKHINQLNYDINERVIMLITKQQPIASDLRMMISSLKIASDLERIGDNASS
                     IANIRLRTKITDDYVLTRLKTMGKLAMLMLKDLDQAFKKKDTVLIREIIERDEDIDDL
                     YSHIINATYLIDNDPFVAAQAHLAARHLERIGDHIINIAESVYFYLTGTHYEQ"
     gene            complement(4886..6700)
                     /gene="pepF"
                     /locus_tag="POB55_002030"
     CDS             complement(4886..6700)
                     /gene="pepF"
                     /locus_tag="POB55_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HDI6500985.1"
                     /translation="MSQGLPLREDVPVSETWDLVDLFKDDQQYYESIDALVQQANQFH
                     HTYATTLNSIEQINTALAELENILIALDRLSNYAELRLSVDTSNIEAQVLSAKLSTTY
                     GKIVSQLSFVESEILELPEEILQQLEESCPYQHYIKQLIKQKPFQLSASVEQVLATLS
                     PTLNSPYDLYGTTKMLDITFDSFEHDGTTYPVDYATFENDYEDNKEPEFRRKSFKSFS
                     DGIRKYQHTTAATYNMQVQQEKIEADLRGFESVIDYLLHSQEVTRDMFDRQIDMIMRD
                     LAPVMQKYAKLLQRIHGLDNMRFEDLKISVDPDYEPEISIEDSKNYIFGALSVLGDDY
                     TNMLREAYDQRWIDFAQNKGKDTGAFCASPYFTHSYVFISWTGKMAEAFVLAHELGHA
                     GHFTLAQKHQPYLESEASMYFVEAPSTMNEMLMANYLFNTSDNPRFKRWVIGSILSRT
                     YYHNMVTHLLEAAYQREVYHKVDQGESLNAPTLNEIMLNVYKQFFGDAVDMTEGAELT
                     WMRQPHYYMGLYSYTYSAGLTIGTVVSQKIKNEGQPAVDAWLETLKKGGSVSPVELAN
                     IAGVDITTEQPLKSTIQYISDLVDEVEKLTDEIEQANN"
BASE COUNT         2382 a         1074 c         1164 g         2214 t
ORIGIN      
        1 cattttaatt ttactaatga agcaatattt tttagattaa caaaaattaa tatttacatt
       61 ttcttaacaa atttttatgt aacatttaca gtttctaaaa atgaggttaa taattcaagg
      121 ttaagataaa gatgtaatca atacaaatac tatttgttgt tcatacaggg aggatatttc
      181 aatgaaaaaa tggcaatttg ttggtactac agctttaggt gcaacactat tattaggtgc
      241 ttgtggtggc ggtaatggtg gcagtggtaa tagtgattta aaaggggaag ctaaaggtga
      301 tggctcatca acagtagcac caattgtgga gaaattaaat gaaaaatggg ctcaagatca
      361 ctcggatgct aaaatctcag caggacaagc tggtacaggt gctggtttcc aaaaattcat
      421 tgcaggagat atcgacttcg ctgatgcttc tagaccaatt aaagatgaag agaagcaaaa
      481 attacaagat aagaatatca aatacaaaga atttaaaatt gcgcaagatg gtgtaacggt
      541 tgctgtaaat aaagaaaatg attttgtaga tgaattagac aaacagcaat taaaagcaat
      601 ttattctgga aaagctaaaa catggaaaga tgttaatagt aaatggccag ataaaaaaat
      661 aaatgctgta tcaccaaact caagtcatgg tacttatgac ttctttgaaa atgaagtaat
      721 gaataaagaa gatattaaag cagaaaaaaa tgctgataca aatgctatcg tttcttctgt
      781 aacgaaaaac aaagagggaa tcggatactt tggatataac ttctacgtac aaaataaaga
      841 taaattaaaa gaagttaaaa tcaaagatga aaatggtaaa gcaacagagc ctacgaaaaa
      901 aacaattcaa gataactctt atgcattaag tagaccatta ttcatttatg taaatgaaaa
      961 agcattgaaa gataataaag taatgtcaga atttatcaaa ttcgtcttag aagataaagg
     1021 taaagcagct gaagaagctg gatatgtagc agcaccagag aaaacataca aatcacaatt
     1081 agatgattta aaagcattta ttgataaaaa tcaaaaatca gacgacaaga aatctgatga
     1141 taaaaagtct gaagacaaaa aataataaga cgcaatttca aatgtgtctt gaaacatgat
     1201 tttgatggtg aatcattatt tagagtacaa agcttgattt atcgagacgc tgattttgac
     1261 attcagttag tctacaagct tatcaactta aaatagtggt tcatcattat tttacaaatc
     1321 taattatttt gggagtaata gaaagaggtt tgattatgac ttcatctact aatgttaaag
     1381 ctttaatcga aaaaaataat aataaaaaag ggaagcataa tgacaaaatt ataccagtta
     1441 ttttagccgc aatttcagcg atttccattt taacaacact aggtatatta atcacattgc
     1501 ttttagaaac catcactttt ttcaccagaa ttccaataac tgaatttcta ttttctacta
     1561 cttggaatcc taccggttca gaccctaagt ttggtatctg ggcattgata atagggactt
     1621 taaaaatcac agttattgcg actatatttg cagttccagt cgggcttggt gcagcaattt
     1681 atttaagtga atatgcaagt gaccgtgcac gacgcataat taaaccgata ttagaaattt
     1741 tagcaggaat accaacaatt gtgtttggtt tctttgcatt aacctttgtt acaccagtat
     1801 taagatcttt cataccaggt cttggagagt ttaatgctat aagtcccggc ttagttgtcg
     1861 gtattatgat tgtccctctc atcacaagtt tgagtgagga tgcaatggca tctgtaccaa
     1921 ataaaattcg agaaggtgcc tatggacttg gagcaactaa attagaagta gcaactaaag
     1981 tcgtacttcc cgcagcaaca tcaggtattg tagcttcaat cgttctcgcg atttcaagag
     2041 caattggaga aacgatgatt gtatcattag cggcaggtag ttcgccaaca gcttcattaa
     2101 gtttaacaag ttcgattcaa acaatgactg gatatattgt tgagatagcg acaggtgatg
     2161 caacatttgg atcaaatatt tattacagta tttatgctgt agggttcaca ctatttatct
     2221 ttaccttaat catgaattta ctttctcagt ggatttctaa gcgttttagg gaggagtatt
     2281 aatatggaaa cgacagataa taatagacaa tcactcgtcg atcaacaact tgtccaaaaa
     2341 catttatcat ccagaacggt taaaaataaa gtgttcaaac tcatattttt agcatgtaca
     2401 ttattaggac ttgtcgtact tattgcgttg ttaactcaaa cattgattaa aggggtaagt
     2461 catttaaatt tacagttttt cactaatttt tcttcttcaa caccatctat ggctggcgtt
     2521 aaaggcgcgt taatcggttc actttggtta atgttaagta tcattccatt atcaatcatc
     2581 ctaggaatag gtacagctat atacttagaa gaatatgcga aaaacaacaa atttactcag
     2641 tttgttaaaa tcagtatttc caatttagct ggtgtaccat cagttgtatt tgggatatta
     2701 ggttacactt tgttcgttgg tggtgcgggg attgaagcct tgaaaatggg taacagtata
     2761 ttggcagcag cgctaacaat gaccttactg atattaccaa ttattattgt ttcaagtcag
     2821 gaagcaatta gagctgtacc taactcagta cgcgaagctt cttatggttt aggtgctaat
     2881 aaatggcaaa cgataagacg tgttgtctta ccagcagcgt tacctggtat tttaactgga
     2941 ttcattttgt ctctttcaag agcactggga gaaacagcgc cacttgtgct aatcggtata
     3001 ccgactatat tattggcaac acctagaagt atattggatc aattttcagc attaccaatc
     3061 caaatattta cttgggcgaa aatgcctcaa gaagaattcc agaatgttgc atcggcaggc
     3121 attatcgttt tactagttat cttaatctta atgaatggcg ttgcgattat tttacgtaac
     3181 aaatttagta aaaaattcta atttaaacaa tcaatctcat ttatctatta aaaagggagt
     3241 tttaaatatg gcgcaaacac ttgcacaaac taaacaaata tctcaaagtc atacgtttga
     3301 tgtctcacaa agtcatcata aaacaccaga tgatacaaac tcacattctg ttatatattc
     3361 aacacaaaat ttagacttat ggtatggcga aaatcatgca ttacaaaata ttaatttaga
     3421 tatttatgaa aaccaaatta ctgccattat aggtccatct ggttgtggca aatcaacata
     3481 tattaaaact ttgaatcgaa tggttgagtt agtaccttct gtgaaaacag ctggtaaaat
     3541 attatatcga gatcaagaca tttttgatca aaaatattct aaagaacaat tacgtacaaa
     3601 tgtgggcatg gtctttcaac aacctaatcc atttccaaaa tcaatatacg ataatattac
     3661 ttacggtcca aagattcacg gtattaaaaa taaaaaagtt cttgatgaaa tcgttgagaa
     3721 atcattacgt ggcgctgcaa tttgggatga attaaaggat aggttgcaca caaatgcata
     3781 tagtttatcc ggtgggcaac aacaacgtgt ttgtatcgcg cgttgtttag caattgaacc
     3841 tgaagtcatt ttaatggatg aaccgacatc agcattagat ccaatctcaa cattaagagt
     3901 agaagagttg gttcaagaac taaaagaaaa gtatacaatt attatggtta cacataatat
     3961 gcaacaagca gctcgtgtat cagataaaac tgcatttttc ttaaatggtt atgtcaatga
     4021 atatgatgat actgataaaa ttttctctaa cccatcaaac aagaaaacag aagattatat
     4081 ttcaggaagg tttggttgat atataatggc aataattaga cagcgatatc aggagcaact
     4141 tgatgattta ataaaggaat tacgtcggtt aggtgcaaat gtctatgtga gtattgaaaa
     4201 tggtataaaa tcattaagta ttgacgatag aggctttgca cgacaaacag ttaaaaacga
     4261 taaacatatc aatcaattaa attatgatat taatgagcga gttatcatgt taattacaaa
     4321 gcaacagccc attgcgagtg atttgcgtat gatgatttct tcattaaaaa tcgcctccga
     4381 tttagaaaga ataggagata atgcatcgag tattgccaat attcgattgc gtacaaagat
     4441 tacagatgat tatgtgttaa cccgtttaaa gacaatgggt aaattagcta tgttaatgtt
     4501 aaaggactta gatcaagcat ttaaaaagaa agataccgta ttaataagag aaataattga
     4561 gcgtgatgaa gatatcgatg acttatatag tcatattatt aacgcaacgt atcttattga
     4621 taacgatcca tttgtcgctg cacaagctca tttagcagca agacatttag aacgtattgg
     4681 tgatcatatt attaacatcg ctgaaagtgt ttatttttat ttaacaggta cacattacga
     4741 acaataactt aaagttatta ctataaaatc ccttacgata aatatatatt tctatcattc
     4801 ataaaccctc aaaaaaccaa gattctcaca attagtaatg tgaaaatctt ggtttatatt
     4861 gttctactat aaattgtctc gcatcttagt tatttgcttg ctcaatttca tctgttaatt
     4921 tttcaacttc atcgactaaa tcagaaatat attgaattgt agatttaagt ggctgttctg
     4981 tagtaatgtc tacacctgca atgtttgcaa gttcgacagg tgatacacta ccaccttttt
     5041 tcaatgtttc taaccaagca tcaacagctg gttggccttc atttttaatc ttttgagaaa
     5101 cgacagttcc gattgttaag ccagcagaat acgtatacga atataatccc atatagtaat
     5161 gaggttgacg catccatgtt aattcagcac cctcagtcat gtctactgca tctccaaaaa
     5221 attgtttata aacatttagc attatttcat ttaatgtcgg cgcatttaaa gattcacctt
     5281 gatctacttt gtgatacact tcacgttgat aagcagcttc taataaatgg gtaaccatat
     5341 tatgataata tgttctagat aaaattgagc caataaccca acgcttaaat cttggattat
     5401 cacttgtgtt aaataaataa ttggccatca acatttcatt cattgtagaa ggggcttcaa
     5461 caaagtacat tgatgcttct gattcaagat atggttgatg tttttgagct aatgtaaaat
     5521 gacctgcatg acctaattca tgtgctaaga caaatgcttc agccatttta ccagtccaag
     5581 aaataaacac atatgaatgt gtaaagtatg gacttgcaca aaatgcgcct gtatctttac
     5641 ctttattttg tgcaaaatca atccatcgct gatcgtatgc ttcacgtaac atgtttgtat
     5701 agtcatcacc taaaacactt aacgcaccga aaatataatt ttttgagtct tcaattgaaa
     5761 tctctggttc ataatcaggg tctacagaaa tcttcaagtc ttcaaaacgc atgttatcta
     5821 atccgtgaat acgttgtaaa agtttagcat atttctgcat aactggtgcc aagtcacgca
     5881 taatcatatc gatttgacgg tcaaacatat cacgcgttac ttcttgacta tgtaataaat
     5941 aatcgatgac tgattcaaat ccacgtaaat cagcttcaat tttttcttgt tgtacttgca
     6001 tattatatgt agccgcggta gtatgctgat attttcgaat cccatcgcta aacgatttga
     6061 aacttttacg tctaaactca ggctctttat tatcttcata atcattttca aacgtagcat
     6121 agtcgacagg gtacgttgta ccatcatgtt caaatgaatc gaatgtaata tctagcattt
     6181 ttgtcgtgcc gtataaatcg taaggactgt ttagcgtagg tgataaagtt gctaatactt
     6241 gttctaccga cgcagataat tggaatggct tttgttttat taactgttta atatagtgtt
     6301 gatatggaca tgattcttct aattgttgaa gtatttcttc tggtaattca agtatttctg
     6361 actctacaaa tgataattgg ctaacaattt taccgtatgt agtagataat ttagcgctca
     6421 atacttgtgc ctcgatatta ctagtatcta cacttaaacg tagttctgca taattactta
     6481 agcgatctaa ggcaattaaa atattttcta attcagctaa agcagtatta atttgttcga
     6541 ttgaatttaa tgttgttgca tatgtatgat gaaattgatt tgcttgttgt actagagcgt
     6601 caatactttc ataatattgt tgatcatctt taaataagtc tactaaatcc catgtttctg
     6661 aaacaggaac atcttctctt aaaggtaaac cttgagacat ataatcactc ctttggtata
     6721 atacattcat tatacataat tttgtctcct aaacgataat gttacgattt ccatgtcgca
     6781 aggaacaatg atttttctaa tagtagatag aatattgctt gaaactgact gcta
//
LOCUS       DAMZKC010000129         6818 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.130, whole genome shotgun sequence.
ACCESSION   DAMZKC010000129 DAMZKC010000000
VERSION     DAMZKC010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6818
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.130"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            135..932
                     /locus_tag="POB55_002031"
     CDS             135..932
                     /locus_tag="POB55_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HDI6500986.1"
                     /translation="MRIMFIGDIVGKIGRDAIETYIPQLKQKYKPTVTIVNAENAAHG
                     KGLTEKIYKQLLRNGVDFMTMGNHTYGQREIYDFIDEAKRLVRPANFPDEAPGIGMRF
                     IQINDIKLAVINLQGRAFMPDIDDPFKKADQLVKEAQEQTPFIFVDFHAETTSEKYAM
                     GWHLDGRASAVVGTHTHIQTADERILPKGTGYITDVGMTGFYDGILGINKTEVIERFI
                     TSLPQRHVVPNEGRSVLSGVVIDLDKEGKTKHIERILINDDHPFSTF"
     gene            1072..2832
                     /locus_tag="POB55_002032"
     CDS             1072..2832
                     /locus_tag="POB55_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HDI6500987.1"
                     /translation="MKPQLSWKVGGQQGEGIESTGEIFATAMNRKGYYLYGYRHFSSR
                     IKGGHTNNKIRVSTTPVHAISDDLDILIAFDQETIDVNHHEMREDSIILADAKAKPVK
                     PEGCHAQLIELPFTATAKELGTALMKNMVAIGATSALMNLNTNTFEELITNMFSKKGD
                     KVVEVNIQALNEGYQLMQSRLPEIDGDFELESTDALPHLYMIGNDAIGLGAIAAGSQF
                     MAAYPITPASEVMEYMIANISKVNGAVIQTEDEIAAVTMAIGANYGGVRAFTASAGPG
                     LSLMMEAIGLSGMTETPLVIINTQRGGPSTGLPTKQEQSDLMQMIYGTHGDIPKIVVA
                     PTDAEDAFYLTMEAFNLAEQYQCPVIVLSDLQLSLGKQTVEKLDYNRIEIKRGEIIQS
                     DIEREEDDKGYFKRYALTSNGVSPRPIPGIKGGIHHITGVEHNEEGKPSESASNRQQQ
                     MEKRMRKIEQLLIESPVEANLQHEDADILYIGFISTKGAIQEGSNRLNQQGIKVNTIQ
                     IRQLHPFPTSVIQDAVNKAKKVVVVEHNYQGQLASIIKMNVNIHDKIENYTKYDGTPF
                     LPHEIEVKGKIIATEIKEMV"
     gene            2833..3699
                     /locus_tag="POB55_002033"
     CDS             2833..3699
                     /locus_tag="POB55_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HDI6500988.1"
                     /translation="MATFKDFRNNVKPNWCPGCGDFSVQAAIQKAAANIGLEPEEVAI
                     ITGIGCSGRLSGYINSYGVHSIHGRALPLAQGVKMANKDLTVIASGGDGDGYAIGMGH
                     TIHALRRNMNMTYIVMDNQIYGLTKGQTSPSSAVGFVTKTTPKGNIEKNVAPLELALS
                     SGATFVAQGFSSDIKGLTKLIEDAINHDGFSFVNVFSPCVTYNKINTYDWFKEHLTSV
                     DDIENYDSTDKQLATKTVIEHESLVTGIVYQDKETPSYESQIKELDDTPLAKRDIKIT
                     EDTFNALTEQFI"
     gene            3793..4086
                     /locus_tag="POB55_002034"
     CDS             3793..4086
                     /locus_tag="POB55_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HDI6500989.1"
                     /translation="MKDTLMSIQIIPKTPNNDNVIPYVDEAIKIIDESGMHFRVGPLE
                     TTVQGNMNECLILIQSLNERMVELECPSIISQVKFYHVPDGITIETLTEKYDE"
     gene            4220..5764
                     /gene="miaB"
                     /locus_tag="POB55_002035"
     CDS             4220..5764
                     /gene="miaB"
                     /locus_tag="POB55_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HDI6500990.1"
                     /translation="MNEEQRKASSVDVLAERDKKAEKDYSKYFEHVYQPPNLKEAKKR
                     GKQEVRYNRDFQIDEKYRGMGNERTFLIKTYGCQMNAHDTEVIAGILEALGYQATTDI
                     NTADVILINTCAIRENAENKVFSEIGNLKHLKKERPDILIGVCGCMSQEESVVNKILK
                     SYQNVDMIFGTHNIHHLPEILEEAYLSKAMVVEVWSKEGDVIENLPKVREGNIKAWVN
                     IMYGCDKFCTYCIVPFTRGKERSRRPEDIIDEVRELAREGYKEITLLGQNVNSYGKDL
                     QDIEYDLGDLLQAISKIAIPRVRFTTSHPWDFTDHMIDVISEGGNIVPHIHLPVQSGN
                     NAVLKIMGRKYTRESYLDLVKRIKDRIPNVALTTDIIVGYPNESEEQFEETLTLYDEV
                     GFEHAYTYLYSQRDGTPAAKMKDNVPLNVKKERLQRLNKKVGHYSQIAMSKYEGQTVT
                     VLCEGSSKKDDQVLAGYTDKNKLVNFKAPKEMIGKLVEVRIDEAKQYSLNGSFVKEVE
                     PEMVIQ"
     gene            5765..6130
                     /locus_tag="POB55_002036"
     CDS             5765..6130
                     /locus_tag="POB55_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RicAFT regulatory complex protein RicA family
                     protein"
                     /protein_id="HDI6500991.1"
                     /translation="MYNKDDVLKQADNIANKIKNLDTIKTYQQIEAQIHQNQTIKTKM
                     DMLKKHQKQAVNFQNYGKQNALEQSEHTIQSIEAEINTLPIVEQFQTSQYEANQLLKM
                     FVSTMETRLNDHNKAKHSD"
     gene            6157..6648
                     /gene="thiW"
                     /locus_tag="POB55_002037"
     CDS             6157..6648
                     /gene="thiW"
                     /locus_tag="POB55_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HDI6500992.1"
                     /translation="MKSRKLAITALLIAINVVLSSIIIIPLGPVKAAPVQHFVNVLSA
                     VIVGPWYGLAQALISSILRVLFGTGTAFAFPGSMIGVLLASMFYIYRKHIFMAAVGEV
                     LGTGVIGSLICIPLAYFLGLQDFFIKPLMITFIVSSAIGSIISYFLLITLKKRGILQR
                     FIK"
BASE COUNT         2523 a          944 c         1289 g         2062 t
ORIGIN      
        1 actaacatat tgaagttttc agacaaagta atgtctcttt ataattgaag aaaaataatt
       61 caaagaattt aatatttact ttaaatatta gaacgtatga attaaactag tatttatgag
      121 aggatgaaca aaacatgaga ataatgttta taggggatat cgtaggtaaa attggacgag
      181 acgcaattga aacgtacata cctcaactga agcaaaagta taaaccaaca gttacaattg
      241 taaatgctga aaatgcagca catggtaaag gtttgactga aaaaatatat aaacaattac
      301 taagaaatgg tgtagatttc atgactatgg gtaatcacac atatggtcaa cgtgaaattt
      361 atgattttat agatgaagca aaacgtctag taagaccagc gaattttccg gatgaagcgc
      421 cgggaattgg tatgagattt atacaaatta atgatattaa acttgcagtt attaatctgc
      481 aaggaagagc gtttatgcca gatattgatg atccttttaa aaaggcagat caattagtca
      541 aggaagcaca agaacaaact ccgtttatat ttgttgattt tcatgcagaa acaacttctg
      601 aaaagtatgc aatgggatgg catttagatg gtagagctag cgctgttgtt ggaacgcata
      661 cacacattca aacagcagat gaacgtattt taccaaaggg gacagggtat ataacggatg
      721 ttggtatgac aggtttttat gacggcattt taggaataaa taaaacagag gtaattgagc
      781 gttttatcac tagtttgcca caaagacatg ttgttccaaa tgaaggtaga agtgtattat
      841 ctggtgttgt tattgattta gacaaagaag gtaaaacaaa gcacatcgaa cgtatattga
      901 taaatgatga ccatccattt tcaacatttt aaaattacgt aagtaaacat tcgaattgga
      961 ccctatcgtc cattagtatg aatttaatat agtaccactg tttacatagt aaatcggtgg
     1021 ttctttttgt tatcatttaa tatgaaatat atccatagga ggcatataac tatgaaacca
     1081 caattatcgt ggaaagttgg cggtcaacaa ggcgaaggta ttgaatcaac tggggaaatc
     1141 ttcgctacgg ctatgaatag aaaaggatat tatttatatg gatatagaca tttttcaagt
     1201 cgtatcaaag gtggacatac gaataataaa attagagttt ctacgacgcc tgttcatgca
     1261 attagtgatg atttagatat tttgattgca tttgaccaag aaacaattga tgttaaccat
     1321 catgaaatga gagaagacag tattatttta gctgatgcca aggctaaacc tgtgaaacca
     1381 gaaggatgtc atgcacagct tattgaatta ccttttacag caaccgctaa agaattaggt
     1441 acagcattaa tgaaaaacat ggttgcaata ggtgctacta gcgcattgat gaatttgaat
     1501 acaaatacat ttgaagaact tattactaat atgttttcta aaaaaggtga caaggtagtt
     1561 gaagtcaata tccaagcatt aaacgaaggt tatcaattaa tgcaatcccg cttacctgaa
     1621 atcgacgggg actttgaatt agagtcaaca gatgcactac cacatctata tatgattggt
     1681 aacgatgcca ttggattagg tgcaattgct gcaggttcac aatttatggc ggcatatcct
     1741 attacacctg cgtctgaagt tatggaatat atgattgcca atatatctaa agtaaacgga
     1801 gcggttattc aaacagaaga tgaaattgct gctgtaacta tggctattgg tgcaaattat
     1861 ggtggagtca gagcgtttac ggctagtgct ggtccaggtt tatctttaat gatggaagca
     1921 attggattat ctggtatgac tgaaacgcca ttagtcatta ttaatactca acgaggtgga
     1981 ccttctactg gattacctac gaaacaagaa cagtcagatt taatgcaaat gatttatggt
     2041 acacatggtg atattccaaa aatagttgta gcaccaactg atgcagaaga tgcattttat
     2101 ttaactatgg aagcctttaa tttagcagaa caatatcaat gccctgttat agttttaagt
     2161 gatttgcaat tatctttagg taaacaaact gttgaaaaat tagattataa tcgcattgaa
     2221 attaaacgtg gtgaaatcat tcaatctgat attgaacgtg aagaagatga taaaggttat
     2281 ttcaagcgtt atgcgttaac atccaatggt gtttctccta gacctatccc cggtattaaa
     2341 ggaggtattc atcatataac tggtgtggaa cacaatgaag aaggtaaacc tagtgaatct
     2401 gcatcaaata gacaacaaca aatggaaaaa cgaatgcgta aaattgagca gttactaatt
     2461 gaatcgccag tagaagctaa cttacaacat gaagatgcag atattcttta tatcggtttt
     2521 atttctacaa aaggtgcaat tcaagaaggt agtaaccgtt tgaatcaaca aggcataaaa
     2581 gttaacacta tacaaattag acaattgcat ccattcccaa caagcgttat tcaagatgca
     2641 gttaataaag cgaagaaagt cgttgtagtg gagcacaatt atcaaggaca attggctagt
     2701 attataaaaa tgaatgtcaa tattcatgat aagattgaaa attatacaaa gtatgatggg
     2761 acacctttcc taccacatga aatcgaagta aaaggcaaaa taattgctac tgaaataaag
     2821 gagatggtat agatggcgac atttaaagat tttagaaata atgttaagcc taactggtgc
     2881 cctggatgtg gcgatttctc agtacaagct gcaattcaaa aagcagccgc aaatataggg
     2941 ttagaacctg aagaagtagc tatcatcacc ggtataggat gttctggccg tctttcagga
     3001 tatattaatt cttatggcgt tcattctatt cacggacgtg cattaccttt agctcaaggt
     3061 gtaaaaatgg cgaataaaga tttaactgtt attgcatctg gaggagatgg tgatggttat
     3121 gctataggta tggggcatac aatccatgct ttaagaagaa atatgaacat gacgtatata
     3181 gtcatggata atcaaattta tggtttgaca aagggacaaa catcgccgtc atcagcagta
     3241 ggatttgtta ctaaaacaac gccaaaaggt aatatagaaa aaaatgttgc gcctttagaa
     3301 ttagcattat catctggtgc cacatttgta gcccaaggtt tttcaagcga tattaaagga
     3361 ttaacaaaac taattgaaga tgcaattaat catgatggat tttcattcgt taatgtcttt
     3421 tcaccatgtg tgacttataa taaaattaac acatacgatt ggtttaaaga acatttaaca
     3481 agtgttgatg acattgaaaa ttatgattct acagataaac aattagcgac taaaactgtt
     3541 attgaacatg aatctttagt aactggtatt gtttatcaag ataaagaaac accatcatat
     3601 gaatctcaaa ttaaagagtt agatgataca ccacttgcta aaagagatat caaaattact
     3661 gaagacacct tcaatgcatt aactgaacaa tttatttaat aattaaaact gagtcgtata
     3721 aatgtattta taacagatcc atttatgcta ctcagttttt tactattaca aaaaataaag
     3781 gagtttttaa aaatgaaaga cacattaatg agtatacaaa taattcctaa aacaccaaac
     3841 aatgacaatg ttatacctta cgtagacgag gcgattaaaa taattgacga atctggtatg
     3901 cattttagag taggtccgtt agaaacgaca gtacaaggaa atatgaatga atgtttaatt
     3961 ttaatacaat cattaaatga acgaatggtg gaacttgaat gtccaagtat tattagccaa
     4021 gttaagtttt atcatgtgcc agatggcatc actattgaaa ctttaactga aaaatatgat
     4081 gaataacatt aaaagtgaag taaactggat ttgaattggc ttgttagaga tgacgtataa
     4141 ctttaactgt ttttgcactt tatagttaaa tttaatataa ttattaaatg atacgggcaa
     4201 atagaaagga ttttgtaaag tgaacgaaga acaaagaaaa gcaagttctg tagatgtttt
     4261 agctgagaga gataagaaag cagaaaaaga ttatagtaaa tattttgaac atgtttatca
     4321 gccgcctaat ttaaaagaag caaaaaaaag aggtaaacaa gaagttcgtt ataatagaga
     4381 ttttcaaatt gatgaaaaat atcgcggtat ggggaacgag cgtacatttt taattaaaac
     4441 atatggatgt caaatgaatg cacatgacac tgaggtcatt gctggtatac ttgaagcatt
     4501 aggctatcaa gcaacgactg atattaacac tgcagatgtt attttaatta atacatgtgc
     4561 gattagagaa aatgccgaga acaaagtgtt tagtgaaata ggtaatttga agcatttgaa
     4621 aaaagaacga cctgatattt taatcggtgt ttgtggttgt atgtcacaag aagagtcagt
     4681 agtgaataaa attttaaaat cgtatcaaaa tgtagatatg atatttggta cacataatat
     4741 tcatcattta ccagaaattt tagaagaagc atacttatct aaagcaatgg ttgttgaagt
     4801 atggtctaaa gaaggagacg ttattgaaaa tcttccaaaa gtccgtgaag gcaacattaa
     4861 agcatgggtc aatattatgt atggttgtga taagttttgt acatattgca ttgttccatt
     4921 tacaagaggt aaagaacgaa gccgcagacc tgaagacatt atagatgaag tacgtgaact
     4981 tgctcgtgaa ggttacaaag aaataacgct tttaggtcaa aatgtaaatt cttatggtaa
     5041 agatttacag gatatagaat atgacttagg agatctttta caagcaattt ctaaaatagc
     5101 gattccaaga gttcgtttca caacaagtca tccttgggac tttacagatc acatgattga
     5161 tgttatttca gagggtggta atatcgttcc tcatatccac ttgccagttc aatctggaaa
     5221 taatgcagta ttaaaaataa tgggtagaaa atatacacga gaaagttatt tggatttagt
     5281 aaaacgaatc aaagatagaa ttcctaatgt agcattaact acagatatta ttgtagggta
     5341 tccaaatgaa tcagaggaac aatttgaaga aactttaact ctgtatgatg aagttggttt
     5401 tgaacatgca tatacgtatt tgtattcaca acgtgatggt acgcctgctg ctaaaatgaa
     5461 agataatgta cctttaaatg tcaaaaagga acgattgcaa cgtttgaata aaaaagttgg
     5521 tcattattca caaatagcta tgagtaagta cgaaggacaa actgtaacag tactttgtga
     5581 aggtagtagt aaaaaagatg atcaggttct tgctggctac actgataaaa ataagctagt
     5641 taatttcaaa gcgcctaaag aaatgattgg taaactagtg gaagtacgaa tagatgaagc
     5701 taaacagtat tcattaaatg gcagttttgt aaaggaagta gagccggaaa tggtgattca
     5761 ataaatgtat aataaagatg acgtgttgaa acaagcggat aatattgcaa ataaaattaa
     5821 aaatttggat actatcaaaa catatcaaca aattgaagca cagattcatc agaaccaaac
     5881 gataaagact aaaatggata tgttaaaaaa gcatcaaaaa caagcagtaa actttcaaaa
     5941 ttacgggaaa caaaatgcgc tagaacagtc ggaacatacc attcaaagta tagaagcaga
     6001 aataaataca ttgcccatag ttgaacagtt tcaaacttca caatatgaag cgaatcaatt
     6061 attgaaaatg tttgtatcaa caatggaaac acgtttaaat gaccataata aagccaagca
     6121 tagtgattaa ttacaaacaa aaaggagaac aatcatatga aatcaagaaa actggctata
     6181 actgcacttt taattgcaat aaatgttgta ttaagcagta ttatcatcat tcctctagga
     6241 ccagttaagg cagcaccagt acagcatttt gtaaatgtat taagtgcggt catagtaggt
     6301 ccttggtatg gattagctca agcgcttata tcatcaattt taagagttct ttttggtact
     6361 ggtacagctt ttgcatttcc aggtagtatg attggagttt tattggctag tatgttttac
     6421 atatatcgta aacatatatt catggccgcg gtcggtgaag tacttggaac tggtgtcatc
     6481 ggaagtttaa tttgtatacc attagcatat ttccttgggc ttcaagactt cttcattaaa
     6541 ccgttaatga ttacgttcat agtctcaagt gctatcggat ctattataag ttatttctta
     6601 ttaattactc taaaaaaacg tggtattctt caaaggttta taaaataatt ttatgatata
     6661 tgatttcaaa cagagtcatt gttcatttta atattgaacg cccttactta attaaatgtc
     6721 ttgaatgtta gaattttaat ttaattctaa acatgctatt gacacatttt attaggtttg
     6781 aggcgttttc ttatacattt aagatgcttc gtgattat
//
LOCUS       DAMZKC010000130         6767 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.132, whole genome shotgun sequence.
ACCESSION   DAMZKC010000130 DAMZKC010000000
VERSION     DAMZKC010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6767
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.132"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            129..590
                     /gene="tsaE"
                     /locus_tag="POB55_002038"
     CDS             129..590
                     /gene="tsaE"
                     /locus_tag="POB55_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HDI6500993.1"
                     /translation="MIKINNLDEMNQFAIFLVEQLKSGDLILLNGDLGAGKTTLTQFI
                     GKALGVRRTINSPTFNIIKSYRGKNLKLHHMDCYRLEDSDEDLGFDEFFEDQAITVIE
                     WSQFIKDLLPATHLSINISTISENTRQIELFAQGEHYEQIKEAIIHEFAAH"
     gene            571..1233
                     /gene="tsaB"
                     /locus_tag="POB55_002039"
     CDS             571..1233
                     /gene="tsaB"
                     /locus_tag="POB55_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HDI6500994.1"
                     /translation="MNSLLIDTSNQPLSVALMQNDEVLAEITTDSKQNHSVQLMPAIS
                     QLFEQSKIAKQQLDAIIVAEGPGSYTGLRIGVTVAKILAYALDVKLYGVSSLKALAAT
                     IDHTDKLLVPVFDARRQAVYTGIFQWQNGQLVTILEDQYMSIEALRAFLEDLNQPFIY
                     IGKDTIKLQDELQGEVIAQLPNASVMYQLIDKPSDVHTFTPKYHKLAEAERNWINSQK
                     NN"
     gene            1206..1670
                     /gene="rimI"
                     /locus_tag="POB55_002040"
     CDS             1206..1670
                     /gene="rimI"
                     /locus_tag="POB55_002040"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDI6500995.1"
                     /translation="MDQQSKEQLNIREMTKEDVPQVFDIERRSFNDSSWTIDAFYHEI
                     EQNNFAKYFVLEFEQQIIGYLGLWIVIDQAQITTVAIDDNYRGYGLGQMLLKYGINYA
                     SHTCDVMSLEVRVNNKVAQHVYENLGFQYGGKRKNYYGEGEDAMVMWVNLND"
     gene            1663..2688
                     /gene="tsaD"
                     /locus_tag="POB55_002041"
     CDS             1663..2688
                     /gene="tsaD"
                     /locus_tag="POB55_002041"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HDI6500996.1"
                     /translation="MTKDILILAVETSCDETSVSFIKNGRDILSNTVLSQIESHKRFG
                     GVVPEVASRHHVEGITTTINEALVDADVSIEDIDAIAVTEGPGLIGALLIGVNAAKAL
                     AFAYDKPLIPVHHIAGHIYANHIEEPLTFPLIALIVSGGHTELVYMKDHLSFEVIGET
                     RDDAVGEAYDKVARTIGLNYPGGPQVDRLAAEGEDTYSFPRVWLDKDSYDFSFSGLKS
                     AVINQLHNQRQKNIPIIEANVATSFQNSVVEVLTFKAIQACKEYGVQRLIVAGGVASN
                     KGLRQSLADQCKVNDIQLTIPSPKLCTDNAAMIGVAGHYLYQQGRFADLALNGHSNID
                     LEEYSAE"
     gene            complement(2790..4400)
                     /locus_tag="POB55_002042"
     CDS             complement(2790..4400)
                     /locus_tag="POB55_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MutS family DNA mismatch repair protein"
                     /protein_id="HDI6500997.1"
                     /translation="MSTNQTFLIFVIAIIPLTSVIGIVGRYMSRQRLLKSMETLWQTI
                     SPLEAFIRPNSHFDYEYKLYKEKFESHSLVDDKTWSDLNMNAIFHKMNYNLTAIGEMK
                     LYACLRGMLSITNKSLLSLFNDNAEFRKNVTYHLALIGKTVYPTFPDQITPVKRQNIL
                     FLCPFLPVISFAVIFINSQVGILLFLMSCLFNIILSATLKRTYEDDLKSIFYASNVLK
                     QGYTISKIKHAPQPEVNFKQFRTARHLTSVLAEVNDEDIGAMVIKLVKLIFMLDYVLF
                     HSIQKSYTTHMNELKNCFDYIAELDNHYALAMYRRTLECYTEPQIDDSNDGIVFSELT
                     HPLIADAVANDFSLSQNILLTGSNASGKSTFMKSIAINIILASAIQTVTASKFVYQPG
                     IVFTSMANADDVLSGDSYFMAELKSIKRIVEIPDNQKIYCFIDEIFKGTNTTERIAAS
                     ESVLSFLHEKSNFRVIAATHDIELAELLKQRYENYHFNEVIENNNIHFDYKIKPGKAN
                     TRNAIELLKITSFPAKIYERAKDNVPKI"
     gene            4489..4617
                     /locus_tag="POB55_002043"
     CDS             4489..4617
                     /locus_tag="POB55_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6500998.1"
                     /translation="MIKTMSIENMCTYQQRVNNLWIKGVDNVIYCGYQVGKQKIND"
     gene            complement(4762..6690)
                     /locus_tag="POB55_002044"
     CDS             complement(4762..6690)
                     /locus_tag="POB55_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDI6500999.1"
                     /translation="MILLQLNHISKSFDGEDIFTDVDFEVKTGERIGIVGRNGAGKST
                     LMKIITGVENYDSGNVSKIKNLKLGYLTQQMTLNSNATVFEEMSKPFEHIKRMESLIK
                     EETDWLSKHANDYDSDTYKTHMSRYESLSNQFEQLEGYQYESKIKTVLHGLNFSEEDF
                     NKPINDFSGGQKTRLSLAQMLLNEPDLLLLDEPTNHLDLETTKWLEDYLRYFKGAIVI
                     ISHDRYFLDKIVTQIYDVALGDVKRYVGNYEEFIQQRDLYYQKRMQEYESQQAEIKRL
                     ETFVEKNITRASTSGMAKSRRKILEKMERIDKPMLDAKSANIQFGFDRNTGNDVMHVK
                     NLEIGYQTAITKPISIEVSKGDHIAIIGPNGIGKSTLIKTIANQQKALNGDITFGANL
                     QIGYYDQKQAEFKSSKTILDYVWDQYPLMNEKDIRAVLGRFLFVQDDVKKIINDLSGG
                     EKARLQLALLMLQRDNVLILDEPTNHLDIDSKEMLEQALQHFEGTILFVSHDRYFINQ
                     LANKVFDLTIDGGKMYLGDYQYYIEKVEEAEALKAHQEEQSVNVQAHEKSMEQSSYHN
                     QKEQRREQRKLERQISECENEIETLETTILQIDEQLTQPEVYNNPQKANELAIQKQDS
                     EQKLEHAMSKWEELQQKL"
BASE COUNT         2223 a          992 c         1163 g         2389 t
ORIGIN      
        1 tctgtttcaa agataagaaa tttaagttga aatttgttac aatataaagg ttaaatataa
       61 agttagatac gattgaacaa tggataatca tttttatgaa tgaaaagcat aatataggag
      121 aatcaacatt gataaagata aataatttag atgaaatgaa tcaatttgct atatttttag
      181 ttgagcaatt gaaaagtggt gatttgattt tacttaacgg agatttagga gcaggtaaaa
      241 caacgttaac gcaatttata ggaaaagctc ttggtgtaag acgtacgatt aattccccga
      301 catttaacat cattaaatca tataggggta aaaatttaaa attgcatcat atggattgtt
      361 atcgcttaga agattctgat gaagatttag gatttgatga atttttcgaa gatcaggcaa
      421 ttactgttat tgaatggagt caatttataa aagatttact tccagcgacg catttatcta
      481 ttaatatttc aacaatatct gaaaatacaa gacaaattga gttgttcgcg caaggagaac
      541 attatgaaca aattaaggag gcaattattc atgaattcgc tgctcattga tacatcgaat
      601 caaccattat cagtagcatt gatgcaaaac gatgaagttt tagctgaaat aactacggat
      661 tctaaacaaa atcattcagt gcaattaatg cctgcgatta gtcagctatt tgagcagagc
      721 aaaatagcta aacagcaatt agatgcgatt attgttgctg aaggtcctgg ttcatatact
      781 ggcttacgta tcggtgttac tgttgctaaa atattagcgt atgcgttaga tgttaaatta
      841 tatggtgttt catcattaaa ggcattggca gcaacaatcg accatactga taaattattg
      901 gttcctgtgt ttgatgcaag acgacaagca gtttatacag gcatattcca gtggcaaaat
      961 ggtcaattag tgactatttt agaagatcaa tatatgtcga tagaggcatt gcgagctttt
     1021 ttagaagatt taaaccaacc atttatttat attgggaaag atactataaa attacaagat
     1081 gaattacaag gtgaagtgat tgcgcagcta ccaaatgcat cagtcatgta tcaattgata
     1141 gataaacctt cagacgtaca tacatttact cctaaatacc ataaattagc tgaggcggaa
     1201 cgaaattgga tcaacagtca aaagaacaat taaacattag agagatgact aaagaagatg
     1261 tgccacaagt ctttgatatt gagcgtcgga gtttcaatga cagttcttgg acaattgacg
     1321 cattttatca tgaaatagag caaaataact ttgcgaaata ttttgtctta gaatttgaac
     1381 aacaaattat tggttattta ggtttatgga ttgttatcga tcaagctcaa attacaacag
     1441 ttgcaatcga tgataattat agaggctatg gtttaggtca aatgctttta aaatacggaa
     1501 taaactatgc aagtcacact tgtgatgtga tgagtttaga agtaagagtt aataataaag
     1561 tggcacaaca tgtatatgaa aatttaggat ttcaatatgg tggtaaacgt aaaaattatt
     1621 atggtgaagg tgaggacgca atggttatgt gggtgaattt aaatgactaa agatatatta
     1681 atactagctg ttgaaacaag ttgtgatgaa acaagcgtta gttttataaa aaatggcaga
     1741 gatattttat caaatacagt tttaagtcag attgaaagtc ataaacgatt tggcggtgtc
     1801 gttcccgaag tggcaagtag acatcacgtt gaaggtataa caacaacaat aaacgaggct
     1861 ctagtggatg ccgatgtatc aatagaagat attgatgcca tagcggttac agaaggccct
     1921 ggactaattg gtgcgttact aataggtgtt aatgcagcca aagcattggc atttgcttac
     1981 gataagccac ttattcctgt tcatcatatt gcaggacata tatatgctaa tcacatagaa
     2041 gagccattaa cattcccgct aattgcactt attgtttcag gtggacatac tgaattagtt
     2101 tatatgaagg atcatttatc atttgaagtc attggtgaaa cacgagatga cgcagtaggt
     2161 gaggcttatg ataaagtggc acgaacaatt ggtttaaatt atccaggtgg tccacaagtt
     2221 gatcggttgg ctgctgaagg tgaagatact tattcattcc ctcgtgtttg gttggataaa
     2281 gatagttatg attttagttt tagtgggttg aaaagtgccg taatcaatca acttcacaat
     2341 caacgacaaa aaaatattcc aatcattgaa gctaacgtag caacgagctt tcaaaatagt
     2401 gttgtagagg tgcttacgtt taaagctatt caagcttgta aagaatatgg tgttcagcga
     2461 ttaattgttg ctggtggcgt ggcgagtaat aaaggattac gtcaatcttt agcggatcaa
     2521 tgcaaagtca atgacattca attaactatc ccaagtccta aattatgcac agataatgct
     2581 gcaatgatag gcgttgccgg ccactatttg tatcagcaag gtcgatttgc tgatttagca
     2641 ttaaatgggc acagcaatat agatttagaa gagtattctg cagaataaaa taaacgtcag
     2701 ttattaacat gattacgatg aacattattg aaactgttca tagtcttctg tcatgtgata
     2761 gctgacgttt ttatatttaa agttaaatgc taaattttcg ggacattatc ttttgctcgt
     2821 tcatatattt ttgctggaaa tgaagtgatt tttaataatt cgatggcatt acgtgtattt
     2881 gctttgccag gcttaatttt gtaatcaaaa tgtatgttat tattttctat tacctcattg
     2941 aaatgataat tttcataacg ttgttttaag agttcagcca actcaatatc atgtgttgct
     3001 gcaataactc taaagttaga tttttcatgt aaaaatgata gtactgattc tgaagcggca
     3061 attcgttcag ttgtgttggt acctttaaaa atttcatcta taaagcagta tattttttga
     3121 ttatctggaa tttcaacaat acgcttaata gattttaatt cagccataaa ataactatcg
     3181 cctgataaca catcatcggc atttgccatt gacgtgaaca caattccagg ttgatataca
     3241 aatttgcttg cggtaactgt ttgtatagcc gatgcaagaa taatgtttat tgctatggat
     3301 ttcataaatg ttgatttacc cgaagcatta gacccagtta acagtatatt ttgtgatagt
     3361 gagaaatcat tggctactgc atctgctatg agtggatgtg ttaactcaga gaatactatg
     3421 ccatcatttg aatcatcaat ttgtggttca gtataacatt ctagcgttct acgatacatt
     3481 gctaatgcat aatgattatc taattcagct atgtaatcga aacaattttt aagctcattc
     3541 atatgtgttg tatagctttt ttgaattgaa tgaaataaaa cataatcaag catgaaaata
     3601 agtttaacta gtttaattac catagctccg atatcttcat cattaacttc agctaaaaca
     3661 ctcgttaaat gacgtgctgt tctaaattgc ttaaaattga cttcaggttg tggagcatgt
     3721 ttaatttttg agatagtata gccttgtttt aaaacattgg atgcataaaa gatagatttt
     3781 aagtcatcct catatgtacg ctttaaagtt gctgataaaa tgatattaaa taggcaactc
     3841 ataagaaata ataaaatacc aacttgtgaa ttaataaata taactgcaaa tgagataact
     3901 ggtaaaaacg ggcatagaaa caatatattt tgacgtttta caggtgtgat ttggtctgga
     3961 aatgttggat aaacagtttt accaatcaaa gctaaatgat atgttacgtt ttttctaaat
     4021 tcagcattat cattaaataa actaagtaat gatttgttcg taattgaaag cattccacgt
     4081 aaacaggcat atagtttcat ttcaccaata gctgttaaat tataattcat cttatgaaag
     4141 attgcattca tatttaagtc ggaccaagtt ttatcatcaa ctaatgaatg tgattcaaat
     4201 ttttccttgt agagcttata ctcatagtcg aaatgtgagt tcggtctgat aaaagcttct
     4261 aatggagaaa tcgtttgcca taatgtttcc atagatttta atagacgttg acgactcatg
     4321 tatcgtccaa caattcctat tacagatgta agtggaataa ttgctataac aaatattaaa
     4381 aatgtttgat ttgtgctcat aagtatctcc ttatttattt ttaataataa taaaaatata
     4441 cttcaatatt attattattt cataaagaaa atgtcttaac aatgaatttt gataaaaaca
     4501 atgtcaatag agaacatgtg tacttatcaa caaagagtga ataacttgtg gataaaaggt
     4561 gttgataacg tcatttattg tggatatcag gtgggaaaac agaaaataaa tgattgatta
     4621 gtgttaattt acttgtggat aactaggata actctgtgga tagctataat atcagcgttt
     4681 ttattgtgaa taaaaaaatt taaaatttgt aaaaaaataa ctctaactat agcgatttta
     4741 cagttagagt tatgtatatt tttatagttt ttgttgtaat tcttcccatt tagacatagc
     4801 gtgttctaat ttttgttcgc tatcttgttt ttggatagct aattcgtttg ctttttgtgg
     4861 attgttataa acttctggtt gggttaattg ctcgtctatt tgtaagatag tagtctctaa
     4921 agtttctatt tcattttcac actcggatat ttgtcgttca agtttgcgtt gttcacgtct
     4981 ttgttctttt tggttatgat acgacgattg ttccatagac ttttcatgtg cttgtacgtt
     5041 aactgactgt tcttcttggt gcgctttcaa cgcttccgct tcctcaactt tttcaatgta
     5101 atattgataa tctcctaaat acatctttcc gccatcaatt gttaaatcaa ataccttgtt
     5161 tgctaattgg ttaataaagt aacgatcatg ggaaacaaat agtattgttc cttcaaaatg
     5221 ttggagtgct tgctctaaca tttcttttga atctatatcg aggtggttgg taggttcatc
     5281 taaaataagt acattgtcac gttgcaacat aagtagtgct agttgtaaac gtgctttttc
     5341 accaccagat aaatcattaa ttatcttttt aacatcgtct tgtacaaata agaaacgacc
     5401 aagaactgca cgaatatctt tttcattcat taacggatat tgatcccaca cataatctaa
     5461 aatcgtttta ctagatttaa attctgcttg tttttgatca taataaccaa tttgtaaatt
     5521 tgcgccgaaa gtaatatcgc cattaagcgc tttttgttga ttagcaatag ttttaattaa
     5581 ggtcgatttt ccaataccat ttggcccaat gattgctata tgatcgcctt tagagacctc
     5641 tatactgata ggtttggtaa ttgcagtttg ataaccgatt tctaaatttt ttacatgcat
     5701 gacgtcatta cctgtattcc ggtcaaagcc aaattgaata tttgcacttt tggcatctaa
     5761 cattggttta tcaatgcgtt ccattttttc taaaatctta cgtctacttt ttgccattcc
     5821 acttgttgaa gcacgggtaa tatttttctc aacaaaagtt tctaatcgtt ttatttctgc
     5881 ttgttgactt tcatattctt gcattcgttt ttgataatat aaatcccgtt gctgtataaa
     5941 ttcctcgtaa ttaccaacat agcgtttgac atcacctaaa gccacatcat aaatttgagt
     6001 aactatttta tctaaaaagt aacgatcatg gctgatgatg acgattgcac ctttaaaata
     6061 acgtagataa tcttcaagcc actttgtcgt ttctaaatct aagtggttag taggttcatc
     6121 taaaagtaat aaatcaggtt cgtttaatag catttgagct aaagataaac gtgttttttg
     6181 gccaccgcta aaatcattga taggtttatt gaaatcttct tcactaaaat ttaacccgtg
     6241 aagtactgtt ttaattttac tttcatattg atacccttct aattgttcaa attgattcga
     6301 taaagattca taacgggaca tatgtgtttt atatgtatca ctatcgtaat catttgcatg
     6361 ttttgataac caatctgttt cttctttgat taagctttcc attcgtttaa tatgttcaaa
     6421 tggttttgac atttcttcaa aaactgttgc attagaattt aaagtcattt gttgagttaa
     6481 ataacctagt tttaagtttt taatttttga cacatttcca gaatcatagt tttctacgcc
     6541 agttataatt ttcatcaatg ttgatttacc ggcgccattt cttcctacga taccaattcg
     6601 ttcaccagtt tttacttcaa aatcaacatc agtaaatata tcttcaccat cgaacgattt
     6661 tgatatatga ttgagttgta aaagtatcat tcgatcttca cctttcgtct ttctcaaatt
     6721 ttattaactt atgaaaaaag tcgtaattat tacattaata accattt
//
LOCUS       DAMZKC010000131         6758 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.133, whole genome shotgun sequence.
ACCESSION   DAMZKC010000131 DAMZKC010000000
VERSION     DAMZKC010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6758
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.133"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            95..1288
                     /gene="fmtA"
                     /locus_tag="POB55_002045"
     CDS             95..1288
                     /gene="fmtA"
                     /locus_tag="POB55_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala esterase FmtA"
                     /protein_id="HDI6501000.1"
                     /translation="MKFNKVKLVIHACVLLFIIISIALIFHRLQTKTHSIDPIHKETK
                     LSDNEKYLVDRNKEKVAPSKLKEVYNSKDPKYKKIDKYLQSSLFNGSVAIYENGKLKM
                     SKGYGYQDFEKGIKNTPNTMFLIGSAQKFSTGLLLKQLEEEHKININDPVSKYLPWFK
                     TSKPIPLKDLMLHQSGLYKYKSSKDYKNLDQAVKAIQKRGIDPKKYKKHMYNDGNYLV
                     LAKVIEEVTGKSYAENYYTKIGDPLKLQHTAFYDEQPFKKYLAKGYAYNSTGLSFLRP
                     NILDQYYGAGNLYMTPTDMGKLITQIQQYKLFSPKITNPLLHEFGTKQYPDEYRYGFY
                     AKPTLNRLNGGFFGQVFTVYYNDKYVVVLALNVKGNNEVRIKHIYNDILKQNKPYNTK
                     GVIVQ"
     gene            complement(1825..2103)
                     /gene="qoxD"
                     /locus_tag="POB55_002046"
     CDS             complement(1825..2103)
                     /gene="qoxD"
                     /locus_tag="POB55_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit IV"
                     /protein_id="HDI6501001.1"
                     /translation="MKHTVGFIASIVLTLLAVYVTLYTSLTFHAKLTIIFGFAFVQAG
                     LQLLMFMHLTEGKDGRLQTFKVIFALVITLCFVVGTYWVMQGGHSSHL"
     gene            complement(2112..2717)
                     /gene="qoxC"
                     /locus_tag="POB55_002047"
     CDS             complement(2112..2717)
                     /gene="qoxC"
                     /locus_tag="POB55_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit III"
                     /protein_id="HDI6501002.1"
                     /translation="MSHDTNTIDSRTHEGELNKLGFWIFITAEFALFGTLFATLLTLQ
                     HGGDYAGKMTTELFELPLVLIMTFALLFSSYTCGIAIYYMRQEKQKLMMFWMIITLLL
                     GLVFVGFEIYEFAHYASEGVNPTIGSYWSSFFILLGTHGCHVSLGIVWAICLLIQIQR
                     RGLDKYNAPKLFIVSLYWHFLDVVWVFIFTAVYMIGMVYSG"
     gene            complement(2707..4695)
                     /gene="qoxB"
                     /locus_tag="POB55_002048"
     CDS             complement(2707..4695)
                     /gene="qoxB"
                     /locus_tag="POB55_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit I"
                     /protein_id="HDI6501003.1"
                     /translation="MNFPWDQLLVKGNWMITMAQIGAPFLVIGLIAVITYFKLWKYLY
                     KEWFTSVDHKKIGIMYLICAVLMFVRGGIDALLIRAQLTVPDNKFLESNHYNEIFSTH
                     GVIMIIFMAMPFIFGLWNIVVPLQIGARDVAFPVLNNVSFWLFFAGMILFNLSFIIGG
                     SPAAGWTNYAPLAGEFSPGPGVNYYLIAIQISGLGTLATGINFFVTILRCKTPTMKFM
                     QMPMFTVTTFITTLIVILAFPPLTVALALMTTDRIFDTAFFTVAHGGMPMLWANFFWV
                     WGHPEVYIVILPAFGIYSEIIPTFARKRLFGHQSMVWATAGIAFLSFLVWVHHFFTMG
                     NGALINSFFSISTMLIGIPTGVKLFNWLLTLYKGRITFESPMLFSLAFIPNFLLGGVT
                     GVMLAMASADYQYHNTYFLVAHFHYTLVTGVVFACLAGLIFWYPKMMGYKLNETLNKW
                     CFWFFMIGFNVCFLPQFILGLDGMPRRLYTYMPSDGWFLLNLISTIGALLMAIGFLFL
                     VVSIVYSHFKSPREATGDNWDGLGRTLEWTTASAIPPKYNFAITPDWNDYDTFVDMKE
                     HGRHYLDNHNYKDIHMPNNTPVGFWIGIFMTIGGFFLIFETVIPALICLFGIFGTMIY
                     RSFQIDHGYHIPAAEVAETEARLREARIKEREAVSHES"
     gene            complement(4695..5795)
                     /gene="qoxA"
                     /locus_tag="POB55_002049"
     CDS             complement(4695..5795)
                     /gene="qoxA"
                     /locus_tag="POB55_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit II"
                     /protein_id="HDI6501004.1"
                     /translation="MSKFKSLLLLFGTLILLSGCSNIEIFNAKGPVASSQKFLILYSI
                     VFMLVICFVVLGMFAIFIYKYSYNKNAESGKMHHNAIIETIWFVIPIIIVAALAIPTV
                     KTLYDYEKPPKSEKDPMVVYAVSAGYKWFFAYPDEHIETVNTLTIPKDRPVVFKLQAM
                     DTMTSFWIPQLGGQKYAMTGMTMNWTLEASQTGTFRGRNSNFNGEGFSRQTFKVNAVS
                     QKDYDKWVKEVKGKKTLDQDTFDKQLLPSTPNKALEFNGTHMAFVDPAADPEYIFYAY
                     KRFNFELKDPNFTSEENMFKDVSDKPLIPARKAQITNANYKRHGMKLMILGNDEPYNN
                     EFKKDESKNAKEMKKISKDAQDQDNDDHGGGH"
     gene            6097..6219
                     /locus_tag="POB55_002050"
     CDS             6097..6219
                     /locus_tag="POB55_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501005.1"
                     /translation="MIERKRKHFNKEFAKSNLSLNNEKWKFVCSKSKHFFKKSS"
     gene            complement(6367..6684)
                     /locus_tag="POB55_002051"
     CDS             complement(6367..6684)
                     /locus_tag="POB55_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5011 domain-containing protein"
                     /protein_id="HDI6501006.1"
                     /translation="MNKLLQSLSALGVSATLVTPNLNADATTNTTPQIKGANDIVIKK
                     GQDYNLLNGISAFDKEDGDLTDKIKVDGQIDTSKSGKYQIKYHVTDSDGAIKISTRYI
                     EVK"
BASE COUNT         2402 a         1059 c         1152 g         2145 t
ORIGIN      
        1 agttaatttt tgtaagttgt attattaggc atctaggttc aaatttttaa cgatttaaat
       61 atataaaatg aatacattgt gaggcaatta tcaaatgaaa tttaataaag taaaactagt
      121 tatacatgcg tgtgtactat tatttatcat tatttctata gcgttaattt ttcatcgatt
      181 acagacgaag acacattcta tagacccaat acataaggaa acaaaattat cagacaatga
      241 aaaatattta gtggatcgta ataaggaaaa ggttgcgccg tctaaactaa aagaggtata
      301 taatagcaag gatcctaaat ataagaaaat tgacaagtat ttacaaagtt cattatttaa
      361 cggttcagta gctatatatg aaaatggcaa attgaaaatg agtaaaggtt atggatatca
      421 agattttgaa aaaggtatta aaaacacacc gaatacgatg tttttaatag gttcagctca
      481 aaaattttca acagggttac tgttaaaaca gttagaagaa gaacataaaa taaatatcaa
      541 tgatccagta agtaaatacc ttccatggtt taaaacatct aagcctatcc cattgaaaga
      601 tttaatgttg catcaaagtg gattatataa atataaatcc tcaaaagatt ataaaaattt
      661 agatcaagca gttaaagcga ttcaaaaacg tggtattgat cctaagaaat acaaaaagca
      721 tatgtataac gatgggaatt atttagtact tgcgaaagta attgaagaag ttacaggtaa
      781 atcttatgct gagaattatt atacaaaaat aggagatcct ttaaaacttc agcacacagc
      841 attttatgat gaacaacctt ttaaaaaata tctagcaaaa ggttatgctt ataatagtac
      901 aggactttca ttcttaagac ctaatatttt ggaccaatac tatggtgcag gtaatttata
      961 tatgacacca acagatatgg gtaaattaat tactcaaata caacaatata aattattcag
     1021 tcctaaaata accaatccat tattacatga gtttggtacg aaacagtatc cagatgaata
     1081 tcgatatggt ttctatgcta agccaacatt gaatagactt aacgggggat tctttggaca
     1141 agtctttact gtttactata atgataagta tgtagttgta cttgcattaa atgtaaaagg
     1201 aaacaatgaa gttcgaatca aacatattta taatgatatt ttaaaacaaa ataaacctta
     1261 caatacgaag ggtgttattg ttcaataatt aatatagaag atataacatg tatatggcat
     1321 taaggcatcg accttatctg accagtatac gagttatatc ttctttttta tagtggtaaa
     1381 aagtttaaag tataaggttg aagaaggatg agtttaaaaa tatgtgttaa ctgataaaag
     1441 gggaaatcat ttggtgagtt ggcatcagac taaaatgaat gaagacgaat acgttggtcc
     1501 atgcgtggtg tgaatgtttt ctaaatctaa ctggaaatga taatttaata atgtacactt
     1561 ttgattgttt aaacatgtac aatttttaaa aacctaggga taattttatt gacgtgaaca
     1621 acattgagat tatctttaga cataaatatg gattttgaag ttactatatt tattagttgt
     1681 ggttgttaat gtattaattg aattgattat aatgattgtc ttataagata tagaagggga
     1741 cttaaccatt taaaaattga aggaaaataa aaaccagcta acaaaagtgt tagctggtta
     1801 aactttacat tattaaaatg ttgtttataa gtgtgaagag tgaccgcctt gcataaccca
     1861 atatgttccg acaacgaaac aaagtgtaat tacaagagca aagataactt tgaatgtttg
     1921 taaacgtcca tctttacctt cagttaaatg catgaacatt aataattgaa gtcctgcttg
     1981 gacgaatgca aagccaaaga taattgtcaa cttcgcgtgg aatgttaatg acgtgtatag
     2041 tgttacgtat actgctaaaa gcgttaatac gatagatgcg ataaatccta cagtatgttt
     2101 cattattgta ctcatccgct atacaccatc cctatcatat atacggcagt aaagatgaaa
     2161 acccaaacaa catctaagaa gtgccagtat aaacttacta taaataattt tggcgcatta
     2221 tatttgtcta atccgcgtcg ttggatttgg attaataaac aaatggccca aacgatacct
     2281 agcgatacgt gacaaccatg cgttcctaat aggataaaga aactagacca gtaagaacca
     2341 attgttgggt taacgccttc tgatgcatag tgtgcgaatt cataaatttc gaatccaaca
     2401 aagactaaac ctaaaagtaa cgtaatgatc atccaaaaca tcattaactt ttgtttttct
     2461 tggcgcatgt agtaaatagc aataccacat gtgtaagaac tgaataataa tgcaaacgtc
     2521 attattaaaa caagaggcaa ttcaaataac tcagtagtca ttttacctgc ataatcgcca
     2581 ccatgttgca aagttaatag tgtcgcaaat agggtaccga ataacgcaaa ttcggctgta
     2641 atgaaaatcc aaaagccaag cttatttaat tcgccttcat gtgtgcgtga atcaatagtg
     2701 tttgtatcat gactcatgac ttacagcctc cctttcttta atacgcgctt ctcttaatct
     2761 agcttcagtt tcagcaactt cagcagcagg gatatgatat ccgtgatcga tttggaaact
     2821 gcgataaatc atagtaccaa aaataccgaa taaacaaatt aatgctggaa ttacagtttc
     2881 gaaaattaag aagaaaccgc cgatagtcat aaagatacca atccagaatc caacaggagt
     2941 attgtttggc atatgaatgt ctttgtaatt atggttgtct aagtaatgac gaccatgttc
     3001 tttcatatca acaaatgtgt cgtagtcatt ccaatctggt gtaatggcaa agttgtattt
     3061 aggtggaatt gctgatgcag tagtccactc tagagtacga ccaaggccat cccagttatc
     3121 tccagttgct tcacgtggag atttgaagtg actgtatacg atactaacaa caaggaataa
     3181 gaatccgatt gccattaata atgcaccgat agttgaaatt aagtttaata agaaccaacc
     3241 atctgatggc atataagtgt ataaacgacg tggcatacca tctaatccaa gaatgaattg
     3301 tggtaagaaa caaacgttaa atccgatcat gaagaaccag aagcaccatt tgtttaatgt
     3361 ttcgtttaat ttgtaaccca tcatctttgg ataccagaag attaaaccag ctaagcaggc
     3421 aaatacaaca ccagtaacca atgtatagtg gaagtgagct actaagaagt acgtattgtg
     3481 atattgatag tcagctgatg ccattgctaa cattacaccc gtaacaccac ctaaaaggaa
     3541 gttagggata aatgctaatg agaatagcat tggtgactca aatgtaatac gtcctttata
     3601 taatgttaat aaccagttaa acaatttcac accagttgga ataccaatca gcattgttga
     3661 aattgagaag aatgagttga ttaacgcacc attacccatt gtgaagaaat ggtgaaccca
     3721 aactaagaaa ctaaggaacg cgataccggc agttgcccat accatacttt gatgtccgaa
     3781 taaacgctta cgagcgaatg tcgggataat ttctgagtaa ataccaaatg ctggaaggat
     3841 aacgatataa acttcagggt gcccccatac ccagaagaag ttagcccaaa gcattggcat
     3901 accgccatgt gcaactgtga agaatgctgt gtcaaatatt ctatcagttg tcattaatgc
     3961 taacgctact gttaaaggag ggaaagcaag aataacaatt aatgtagtaa taaatgttgt
     4021 tactgtaaac attggcattt gcataaactt catagttggt gttttacatc ttaaaattgt
     4081 tacaaagaag ttgatacctg tagctaaggt accaagccct gaaatttgta tagctattaa
     4141 gtaatagtta acacccggac caggactgaa ttcacctgct agtggcgcat agtttgtcca
     4201 accagctgct ggtgaaccac caataataaa tgacaggttg aataaaatca tacctgcaaa
     4261 gaatagccag aaacttacgt tgtttaatac tgggaatgca acatcacgtg ctccaatttg
     4321 taatggaaca acgatattcc ataaaccaaa gataaatggc attgccatga agataatcat
     4381 gattacacca tgtgtactaa aaatttcgtt atagtggtta gattctaaaa atttgttatc
     4441 aggtactgtt aattgcgcac gaataagtaa cgcatcaata ccaccacgga cgaacattaa
     4501 tacggcacag attaaataca taataccgat tttcttatgg tctacagatg tgaaccattc
     4561 tttgtaaaga tatttccata atttaaagta agtaattact gcgattaaac caataactaa
     4621 gaatggggca ccaatttgtg ccattgtaat catccagtta cctttaacta gtaattgatc
     4681 ccatggaaaa ttcattaatg tccacctcca tgatcatcat tgtcttgatc ttgcgcatct
     4741 tttgaaattt tcttcatttc tttcgcattt ttcgattcat ctttcttgaa ctcattgtta
     4801 tatggttcgt catttccaag aatcatcaac ttcataccat gtcgtttata gttcgcattt
     4861 gtaatttgag ctttacgagc aggtattaat ggtttgtctg atacatcttt aaacatattt
     4921 tcttcactag tgaagtttgg atctttcaat tcgaaattga aacgtttata tgcatagaag
     4981 atgtattctg gatcggctgc tggatcaaca aacgccatat gtgtaccatt aaattctaaa
     5041 gctttattag gtgtgcttgg taataattgt ttatcaaatg tatcttgatc taacgttttc
     5101 ttacctttaa cttctttcac ccatttgtcg tagtcttttt gactaacggc atttacttta
     5161 aatgtttgac gtgagaatcc ttcaccattg aagttagagt tacgacctct gaacgtacca
     5221 gtttgagatg cttctaacgt ccaattcatt gtcatgccag tcatggcata tttttgacca
     5281 cctaattgtg gaatccagaa acttgtcatt gtatccatag cttgaagctt aaatacaaca
     5341 ggacgatctt tagggattgt taatgtgtta acagtctcta tatgttcatc tggataagca
     5401 aagaaccatt tgtatcctgc acttactgca tatacaacca ttggatcttt ctcactcttc
     5461 ggtggttttt cgtaatcgta taaagtttta actgtaggaa tagctaaagc agcaacgatt
     5521 atgataggta ttacaaacca tattgtttca atgatggcat tatggtgcat cttaccagat
     5581 tcggcattct tattataact atacttgtaa ataaaaatgg cgaacatgcc aagtacaacg
     5641 aaacaaataa caagcatgaa gacgattgaa taaagaatca agaacttctg actacttgct
     5701 actggccctt ttgcgttgaa aatttctata tttgaacaac cactaagtaa aattagtgtg
     5761 ccaaataata gaagcaaaga cttaaatttt gacacttttt tgacctccta atactacaaa
     5821 tgtagggctt aacattaatt ttaagttatt acacaatatt tacaagggct tatgggaaaa
     5881 aaattaataa aattgtatca aaaatgttga taaatcaagg tgtgacgtgg gttcacacat
     5941 ttgttaaaat tatgtgtaca ttttgtgact aatagcgttt ttgaaaaaat tgcttcatat
     6001 ttaaaaggaa agttgagaat agctttcaat taaaggttaa ttgaatgaaa ataaaaaaag
     6061 tttgaaattg tctaataaat tgaaaatgat ggctagttga ttgaaaggaa gcgcaaacat
     6121 tttaataagg aatttgccaa aagtaattta tcgttaaaca atgaaaagtg gaaatttgtt
     6181 tgttcaaaat caaaacattt ttttaaaaag agcagttaag ttttaataat ttgataggta
     6241 gtcctttggt atacagaaaa aatacaatat ttgggttaaa gttgcagatg aagttagaat
     6301 aacaaataaa atggtagcaa aacatgttcg tttgctacca ttttatttgc agtatagtga
     6361 tgagggctat ttaacctcaa tatacctagt ggaaatttta attgcaccat ctgaatcagt
     6421 gacatgatat ttaatttgat atttaccaga tttagatgta tcaatttggc catcgacttt
     6481 aattttatcg gttaaatctc catcttcttt atcaaatgca cttatgccgt ttagaaggtt
     6541 ataatcttga cctttcttaa taacgatatc attagcgcct ttaatttgtg gtgtagtatt
     6601 cgtcgttgca tctgcattta aatttggtgt tactagtgta gcagaaacac cgagggctga
     6661 taatgactgt aatagtttat tcataatgta acctccgaat gataagtcat ttctgagtta
     6721 gctttactat accttaattt atattattta cacatcat
//
LOCUS       DAMZKC010000132         6578 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.134, whole genome shotgun sequence.
ACCESSION   DAMZKC010000132 DAMZKC010000000
VERSION     DAMZKC010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6578
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.134"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            41..433
                     /locus_tag="POB55_002052"
     CDS             41..433
                     /locus_tag="POB55_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HDI6501007.1"
                     /translation="MKKVINVVGAIIFSDNKILCAQRSEEMSLPLMWEFPGGKIEKNE
                     TEKEALIREIREEMKCDLIVGDKVITTEHEYDFGIVKLTTYKCTLNKELPTLTEHKSI
                     EWLSINELDKLNWAPADIPAVNKIMTEG"
     gene            435..3296
                     /locus_tag="POB55_002053"
     CDS             435..3296
                     /locus_tag="POB55_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3427 domain-containing protein"
                     /protein_id="HDI6501008.1"
                     /translation="MSRLLNDFNQSLKKGFIDKDISHKGNYTPKLLVNNKNEKVLSTI
                     IDELQKCETFYFSVAFITESGLASLKAQLLDLSNKGVKGKILTSNYLGFNSPKMYGEL
                     LKLKNVEVRLTDIAGFHAKGYIFEHKDYSSMVIGSSNLTSNALKVNYEHNVLLSTMKN
                     GDLVDSVKNEFELLWQKSTPLTQQWIKSYKESFEYRSLEKLAEVEQTQMLLADKVKKS
                     VEIVPNLMQAEALRSLKAIRDKAKDKALIISATGTGKTILCALDVREVNPNKFLFIVH
                     NEGILNRAKEEFKKVLPIKNDSDFGLLTGKHRDVDAKYLFATIQTLSRDDNFKQFDEN
                     EFDYIVFDEAHRSAASTYQRVFNYFKPKFMLGMTATPERSDELSIFELFDYNIAYEIR
                     LQAALESDILCPFHYFGVTDYVHQGIKEDDVTKLRYLTSDERVNYIIQKTDYYGYSGE
                     ILQGLIFVSSKKEAYDLADKLSSKGIKSVALTGDDSVNYRQIVIEKLKEGKINYIITV
                     DLFNEGIDIPEVNQVVMLRPTESSIIFIQQLGRGLRKSSNKEYVTVIDFIGNYKTNYL
                     IPIALSGDQSQNKDNYKKFLTNNDSINGVSTINFEEVAKKQIYNSLDAVSLNQNKLIL
                     KAYEEVENRLGHMPLLMDFIQQHSIDPSVIFSKFSNYYEFLVRYNKIDTLLTENESKN
                     LVFFSRQIAPGLKRIDSLVLEELLKNELTYDELKNKMLNEVKDITEDDIDTSLRILDF
                     SFYNAGIEKIYGSPIIECNERMIRLSDAFTNALSNQTFKIFLEDLIELSKYNNEKYQK
                     GKNGLILYNKYSREDFSKIFNWNKNGSSVIMGYMIRSQEMPIFITYDKHEDISDSTKY
                     EDEFLSQDELKWFTKSNRTLKSKEVQKILSHRAKGIKMYIFVQKKDDDGIYFYYLGTA
                     GYIEGSEKQDKMPNGSNVVTMDLALDKAVRDDIYRYITN"
     gene            3602..4360
                     /locus_tag="POB55_002054"
     CDS             3602..4360
                     /locus_tag="POB55_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDI6501009.1"
                     /translation="MIHSKRLRLWLYLVLLAVFISACGMKEDKQIKENFNKTLSLYPT
                     KNLDDFYDKEGFRDQEFEKGDKGTWIVDSEMVVELKDKKMESRSMVLYINRNTRTTKG
                     NFIVRELWEDSKGYAQSKDTKYPVKMEHNRIIPTKPIADDKLRKEIENFKFFVQYGDF
                     KDINDYKDGDISYNPNVPSYSAEYQLSNNDYNVKQLRKRYDIPTKKAPKLLIKGDGDL
                     KGSSIGHKNLEFTFVENKEENIYFTDSINFKPTK"
     gene            4472..5293
                     /locus_tag="POB55_002055"
     CDS             4472..5293
                     /locus_tag="POB55_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDI6501010.1"
                     /translation="MMHSKKLTLGICLVLLIILIVGYVIMTKANGQNAQIKDTFNQTL
                     KLYPTKNLDDFYDKEGFRDQEFKKGDKGTWIVNSGMNIQLKGGALKSREMVLYINRNT
                     RTTKGYFIVGEITKDKKGYTHDKDKKGYTHDKDKKYPVKMEHNKIIPTKPIKDEKLKK
                     EIENFKFFVQYGNFKDFKDYKNGDISYNPNVPSYSAKYQLSNDDYNIQQLRKRYDIPT
                     KKAPELLLKGDGDLKGSSIGSKDLEFTFVQNKRENIYFTDSVEFTPSEDTSYESN"
     gene            5407..6222
                     /locus_tag="POB55_002056"
     CDS             5407..6222
                     /locus_tag="POB55_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDI6501011.1"
                     /translation="MIHSKRLKLCLCLIILSVFIGACGMKKEESSKDKQIKENFNKIL
                     SLYPTKNLEDFYDKEGFRDEEFEKGDKGTWIIHSKMIIETNNSNMESRGMVLYINRNT
                     RTTKGNFVVREITEDSKGYSHSKDTKYPVKMEHNRIIPTKPIADDKLRKEIENFKFFV
                     QYGDFKDINDYKDGDISYNPNVPSYSAKYQLSNDDYNVKQLRKRYNIPTNKAPKLLLK
                     GDGDLKGSSVGSKNLEFTFVENKEENIYFTDSVQYTPSEDTSYESNGISNKSW"
     gene            complement(6205..6327)
                     /locus_tag="POB55_002057"
     CDS             complement(6205..6327)
                     /locus_tag="POB55_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501012.1"
                     /translation="MSYIIKFTFKFFEMATYISQLTLDIELVSSELLVILPGFI"
BASE COUNT         2694 a          736 c         1152 g         1996 t
ORIGIN      
        1 aataacaatt aagatgattt taaattgaag ggtgagtctt atgaaaaaag taatcaatgt
       61 agtaggagct attatttttt ctgataacaa aattctttgt gcacagagaa gtgaagaaat
      121 gagtctacct ttaatgtggg aatttcctgg cggtaagatt gaaaagaatg aaactgaaaa
      181 agaagctttg atcagagaaa ttagagaaga aatgaaatgt gatctaattg ttggagacaa
      241 agtcataact acagaacatg aatatgattt tggcattgtt aagttaacaa catacaaatg
      301 tactttaaac aaagagttac caactttaac tgaacataag agtattgaat ggttgtcaat
      361 aaacgaactt gataaattaa attgggcccc agcggatata ccagccgtta ataaaattat
      421 gactgaggga taatatgagt agattactaa atgatttcaa tcaatcttta aaaaaaggtt
      481 ttatagacaa agatatttct cataaaggga attatacacc gaagctttta gtaaataata
      541 aaaatgaaaa agtcctttca acaattatag atgaacttca aaaatgtgaa acgttctatt
      601 tttctgtagc ttttataaca gaaagcggat tagccagctt aaaggctcaa ctattagatt
      661 taagcaataa gggtgttaaa gggaaaatat taacatctaa ttacttagga tttaatagtc
      721 ctaaaatgta tggagaatta cttaaattaa aaaatgtaga ggtaagatta actgatattg
      781 ctggattcca tgccaaagga tatatttttg aacataagga ttatagttct atggtaatag
      841 gaagctctaa tttaacatct aatgcgttaa aggttaatta tgagcataat gttttactgt
      901 ctactatgaa aaatggcgat ttagttgata gtgtaaaaaa tgaatttgaa ttgttatggc
      961 aaaagagtac cccactgact cagcaatgga ttaaatcata taaagaatca tttgagtacc
     1021 gctccttaga aaaattagcg gaggttgaac aaactcaaat gctgctagct gataaggtga
     1081 aaaaatcagt ggaaattgta cccaatttaa tgcaagcaga agcattaagg tcattaaaag
     1141 ctattaggga taaagccaaa gataaggcgt taataatatc tgcaactggt acaggtaaaa
     1201 cgattttatg tgcattagat gttagagaag taaaccctaa taaattttta tttattgttc
     1261 ataatgaggg gattttaaat agagctaagg aagaatttaa aaaagtatta cctataaaaa
     1321 atgatagtga ttttggattg ttgacaggaa aacatagaga tgtcgatgcc aaatatttat
     1381 ttgcaaccat tcaaacatta tctagagatg ataattttaa gcaatttgat gaaaatgaat
     1441 ttgattatat tgtttttgat gaagcacatc gttctgctgc atctacttat caacgagtat
     1501 ttaactactt caaacctaag ttcatgctgg gaatgactgc aacaccagaa agatcagatg
     1561 aattaagtat atttgaactg tttgattata atattgcgta tgaaataagg ttacaagcag
     1621 cattagaaag tgatatttta tgtccatttc attattttgg tgtgactgat tatgtacatc
     1681 aaggtattaa agaagatgat gtaactaaac tgagatattt aacttctgat gaaagagtta
     1741 attatattat tcaaaagaca gattactatg gatattcagg tgaaatttta caaggattaa
     1801 tttttgtaag tagcaaaaaa gaagcttatg atttagcgga taaactaagt tcaaaaggga
     1861 ttaaaagtgt tgctttaaca ggagatgata gtgttaatta tcgccaaatt gtaatagaaa
     1921 aattaaaaga aggtaagatt aactatatta taactgtaga tctttttaac gaaggtattg
     1981 atattccaga agtcaatcaa gttgtaatgt tgagaccaac agagtctagt attatattca
     2041 ttcagcaact tggtagagga ttaagaaaaa gttcaaataa agagtatgtt acagtaattg
     2101 attttattgg taattataag acaaattatt taattccgat tgcgctttct ggggatcaat
     2161 cgcaaaataa agataattat aagaagtttt taacgaataa cgattcgatt aatggagtat
     2221 ctacaattaa ttttgaagaa gttgctaaaa aacagattta caattcattg gatgcagtat
     2281 cattaaatca aaataaatta atattaaaag cttatgaaga agttgaaaat agattgggac
     2341 acatgccgtt actaatggat ttcatacaac aacattctat agatccaagc gttatatttt
     2401 ctaaatttag taattattac gagttcttag tgagatataa caaaatagat acattattga
     2461 cggaaaatga atctaaaaat ctggttttct tttcaagaca aatagcacct ggattgaaaa
     2521 gaatcgatag tttagtttta gaagaacttt taaaaaatga attaacatat gatgaattaa
     2581 aaaataaaat gttgaacgaa gttaaggata taacagaaga tgatatagat acttcattaa
     2641 gaattttaga tttttcattt tacaacgcag gaattgaaaa aatctatgga agtcccatta
     2701 tagaatgtaa tgaaagaatg ataagactaa gcgatgcatt tactaatgct ctaagtaatc
     2761 aaacatttaa aatattttta gaagatctta ttgaattatc aaagtataac aatgaaaaat
     2821 accaaaaagg taaaaatgga ctgattttat ataataaata ttcaagagaa gattttagca
     2881 aaatatttaa ttggaataaa aatggttcgt ctgtaatcat gggatacatg ataagaagcc
     2941 aagaaatgcc aatatttatc acttatgata agcatgagga tataagtgat agcacaaaat
     3001 atgaagatga atttttaagt caagatgaat taaaatggtt tactaaatct aatagaacgc
     3061 taaaatcaaa agaagttcag aaaattttgt cccatagagc aaaaggaatt aaaatgtata
     3121 tttttgtaca gaaaaaagat gatgatggta tatattttta ttatttagga actgctggat
     3181 atatagaagg ttcagagaag caagataaaa tgccgaatgg ttcaaacgta gtaacgatgg
     3241 atttagcact cgataaagct gtaagagatg atatatatcg ctatataaca aattaatgta
     3301 aaccgaaaaa tgaatgttag taaagatgta tttaaacatc aattttaata agtaaagaca
     3361 ttgagtttca gtgttattat tttaaatata agcgcaattt atcttaatta attagttggt
     3421 taggccaata aaaattataa agatatttgc gctttttaaa taatttgtga tgttgatatg
     3481 tgaaaaatac aactaacaat taaaagccaa gaggatttaa tcataaaatt ccctctagta
     3541 ttattaatat tggaatttga acttaacttt gcatcaaaaa taaaaatggg ggtgtgaaat
     3601 gatgattcat tcaaaaaggt tgaggctctg gctgtattta gtattattag ctgtttttat
     3661 tagcgcgtgt ggtatgaaag aagataaaca aattaaagaa aatttcaaca aaacattgag
     3721 tttatatcca accaagaatc tcgatgattt ttatgataaa gaaggatttc gagatcaaga
     3781 gtttgaaaaa ggagataaag ggacttggat tgttgattct gagatggtag ttgagctgaa
     3841 ggataaaaaa atggaatcta gaagtatggt gctctatata aatcgtaata ctagaacaac
     3901 gaaaggtaat tttattgtta gagaattgtg ggaagatagt aaagggtatg cacaaagtaa
     3961 agatacaaaa tatccagtga aaatggaaca taatcgaatt atcccaacca aaccgatagc
     4021 tgatgataaa ttaagaaaag aaatcgaaaa ctttaagttt tttgtacaat acggtgactt
     4081 taaagatatt aatgattata aagatggtga catatcatat aatcccaatg tacctagtta
     4141 ttcggcggaa tatcaattga gtaacaatga ctacaatgtg aagcaactta gaaagaggta
     4201 tgatatacca actaagaaag cacctaaatt attaataaaa ggcgatggtg atttaaaagg
     4261 atcatctata ggacacaaaa atttagagtt tacctttgta gaaaataaag aagaaaacat
     4321 ctattttacg gatagtatta atttcaaacc tactaaatag ggttaataga agtttggaat
     4381 gaaaatttca taaagtgttc aaaatgattt ttcgttttag ttgtgtaaat taaagattga
     4441 gtcagtatta aaaatataag gtggagaaat gatgatgcat tcaaagaaat taacgctcgg
     4501 tatatgcttg gtattactca ttatattgat tgtaggttat gtcattatga caaaagcaaa
     4561 tggtcaaaac gcccaaatta aagacacatt taatcaaaca ttaaaattat atccaaccaa
     4621 aaatctcgat gatttttacg ataaagaagg ctttcgagat caagaattta aaaagggtga
     4681 taaaggtact tggatagtta attctggaat gaacatccaa ttaaaaggag gagccttaaa
     4741 atccagagag atggtacttt acatcaatcg taacactaga acaacgaaag gatattttat
     4801 agtaggtgag ataactaaag ataaaaaagg atatacacat gataaagata aaaaaggata
     4861 tacacatgat aaagataaaa aataccctgt caagatggaa cataataaaa tcataccaac
     4921 aaaacctata aaagacgaga agttaaaaaa agaaattgaa aacttcaagt tttttgtaca
     4981 atatggcaac tttaaagatt ttaaagatta taaaaatggg gatatttcat acaatccaaa
     5041 cgtaccaagt tattcggcaa aatatcaatt aagtaatgat gattataata tccaacaatt
     5101 aagaaaaaga tatgatatac cgactaaaaa agcaccagaa ctattgttga aaggtgatgg
     5161 cgatttaaaa ggttcatcca taggctctaa agacttagaa tttacctttg tacaaaataa
     5221 aagagagaat atctatttta cagattcggt tgaatttact ccaagcgagg atacaagtta
     5281 tgagtcaaac taaatagggt taatagaagt ttggattgaa aatttcataa agtgttaaaa
     5341 aatttttcgc tttaaatgta taaattaaag attgagtcag tattaaaaat ataaggtgga
     5401 gaaatgatga ttcattcaaa aaggttaaaa ttgtgtctat gcctgataat actatctgtt
     5461 tttataggcg cttgcggaat gaaaaaagaa gaaagtagta aagataaaca aattaaagaa
     5521 aatttcaaca aaatattgag tttatatcca accaaaaatc ttgaagactt ctatgataag
     5581 gaaggctttc gtgacgaaga gtttgaaaaa ggtgacaaag gcacttggat aattcactct
     5641 aaaatgatta ttgagacaaa taatagcaat atggaatcta gaggaatggt gctctatata
     5701 aatcgtaata ctagaacaac gaaaggtaat tttgttgtta gagaaataac cgaggatagt
     5761 aaagggtatt cacatagtaa agatacaaaa tatccagtga aaatggaaca taatagaatc
     5821 attccaacca aaccgatagc tgatgataaa ttaagaaaag aaatcgaaaa ctttaaattc
     5881 tttgtacaat acggtgactt taaagatatt aatgattata aagatggtga catttcatat
     5941 aatccgaatg tgccaagtta ttcagcaaag tatcaattaa gtaatgatga ctataatgtg
     6001 aagcaacttc gaaaaagata taatattcca accaacaaag ctccgaaact attattgaaa
     6061 ggtgatggtg atttaaaagg ctcatctgta gggtcaaaaa atctagaatt tacctttgta
     6121 gaaaataaag aagaaaacat ttattttaca gattctgtac aatatactcc aagtgaggat
     6181 acaagttatg agtcaaacgg aatatcaaat aaatcctggt aatataacaa gtaactctga
     6241 agaaacaagt tcgatatcta aagtgagctg tgaaatatag gtagccattt caaaaaattt
     6301 aaaggtgaat ttgataatgt agctcaagga gattggggta aaaaggcgaa gaatgaactg
     6361 gatgatatta gtaagaaatt aaaaaatatt caaagaacgg aagtttaata ttctagccga
     6421 ttcctgaaac taagacagca tgcgttcatt catgccatta ttaatataag caccgcaaca
     6481 aaaaagcttc taatgtgata tcggaacctc ggattccata tcatgttaga agcttttaat
     6541 gtctaaagaa catctacatt ttatcatatt ttcttact
//
LOCUS       DAMZKC010000133         6543 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.135, whole genome shotgun sequence.
ACCESSION   DAMZKC010000133 DAMZKC010000000
VERSION     DAMZKC010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6543
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.135"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            64..1290
                     /gene="ftsW"
                     /locus_tag="POB55_002058"
     CDS             64..1290
                     /gene="ftsW"
                     /locus_tag="POB55_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HDI6501013.1"
                     /translation="MKNFRSILRYIGKTSKFIDYPLLVTYIVLSLIGLVMVYSASMVP
                     ATKGTLTGGIDVPGTYFYNRQLAYVIMSFIIVFFIAFLMNVKLLSNIKVQKGMIITIV
                     SLLLLTLVIGKDINGSKSWINLGFMNLQASELLKIAIILYIPFMISKKMPRVLSKPKL
                     ILSPIVLALGCTFLVFLQKDVGQTLLILIILVAIIFYSGIGVNKVLRFGIPAVLGFLV
                     VFVIALMAGWLPSYLTARFSTLTDPFQFESGTGYHISNSLLAIGNGGVFGKGLGNSAM
                     KLGYLPEPHTDFIFAIICEELGLIGGLLVITLEFFIVYRAFQFANKTSSYFYKLVCVG
                     IATYFGSQTFVNIGGISATIPLTGVPLPFISFGGSSMISLSIAMGLLLIVGKQIKVDQ
                     QRKKQQQKVDIRRQFN"
     gene            1844..5296
                     /locus_tag="POB55_002059"
     CDS             1844..5296
                     /locus_tag="POB55_002059"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HDI6501014.1"
                     /translation="MKQIKKLLVANRGEIAIRIFRAAAELDISTVAIYSNEDKSSLHR
                     YKADESYLVGSDLGPAESYLNIERIIDVAKQANVDAIHPGYGFLSENEQFARRCAEEG
                     IKFIGPHLEHLDMFGDKVKARTTAIKADLPVIPGTDGPIKSYELAKEFAEEAGFPLMI
                     KATSGGGGKGMRIVREESELEDAFHRAKSEAEKSFGNSEVYIERYIDNPKHIEVQVIG
                     DEYGNIVHLFERDCSVQRRHQKVVEVAPSVGLSPTLRQRICDAAIQLMENIKYVNAGT
                     VEFLVSGDEFFFIEVNPRVQVEHTITEMVTGIDIVKTQILVAAGADLFGEEINMPQQK
                     DITTLGYAIQCRITTEDPLNDFMPDTGTIIAYRSSGGFGVRLDAGDGFQGAEISPYYD
                     SLLVKLSTHAISFKQAEEKMVRSLREMRIRGVKTNIPFLINVMKNKKFTSGDYTTKFI
                     EETPELFDIQPSLDRGTKTLEYIGNVTINGFPNVEKRPKPDYELASIPTVSSSKIASF
                     SGTKQLLDEVGPKGVAEWVKKQDDVLLTDTTFRDAHQSLLATRVRTKDMINIASKTAD
                     VFKDGFSLEMWGGATFDVAYNFLKENPWERLERLRKAIPNVLFQMLLRASNAVGYKNY
                     PDNVIHKFVQESAKAGIDVFRIFDSLNWVDQMKVANEAVQEAGKISEGTICYTGDILN
                     PERSNIYTLEYYVKLAKELEREGFHILAIKDMAGLLKPKAAYELIGELKSAVDLPIHL
                     HTHDTSGNGLLTYKQAIDAGVDIIDTAVASMSGLTSQPSANSLYYALNGFPRHLRTDI
                     EGMESLSHYWSTVRTYYSDFESDIKSPNTEIYQHEMPGGQYSNLSQQAKSLGLGERFD
                     EVKDMYRRVNFLFGDIVKVTPSSKVVGDMALYMVQNDLDEQSVITDGYKLDFPESVVS
                     FFKGEIGQPVNGFNKDLQAVILKGQEALTARPGEYLEPVDFEKVRELLEEEQQGPVTE
                     QDIISYVLYPKVYEQYIQTRNQYGNLSLLDTPTFFFGMRNGETVEIEIDKGKRLIIKL
                     ETISEPDENGNRTIYYAMNGQARRIYIKDENVHTNANVKPKADKSNPSHIGAQMPGSV
                     TEVKVSVGESVKANQPLLITEAMKMETTIQAPFDGVIKQVTVNNGDTIATGDLLIEIE
                     KATD"
     gene            complement(5436..6347)
                     /locus_tag="POB55_002060"
     CDS             complement(5436..6347)
                     /locus_tag="POB55_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000467197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme A synthase"
                     /protein_id="HDI6501015.1"
                     /translation="MFGKKNLKWLGVVATLMMTFVQLGGALVTKTGSADGCGSSWPLC
                     HGALIPEFFPIDTIIELSHRAVSALSLLMVLWLVITAWKHIGYIKEIKPLSIISVGFL
                     LLQALIGAAAVIWQQNDYVLALHFGISLISFSSVFLITLIIFSIDQKYEADELYIKKP
                     LRRLTWLMAIIIYCGVYTGALVRHADASLAYGGWPLPFHDLVPHSEQDWVQLTHRIMA
                     FIVFTIIMITYIHAVKNYPNNRTVHYGYTAAFILVILQVITGALSIMTNVNLLIALFH
                     ALFITYLFGMTTYFIMLMLRSVRSDKQ"
BASE COUNT         2314 a          955 c         1223 g         2051 t
ORIGIN      
        1 taaaattaaa gtataaaacg aaatcatttg aagtataaat tgtagaattg gatgactgaa
       61 tatatgaaga attttagaag tattttacgg tatattggta aaacctcaaa gtttattgat
      121 tatccgttat tagttacata tattgtattg agtttaattg gattagtgat ggtatatagt
      181 gcaagtatgg ttccagcaac taaaggcaca ttgactggtg gtatcgatgt tccaggaacg
      241 tatttttaca accgacaatt agcatatgtc ataatgagtt ttataattgt attttttatt
      301 gcatttttaa tgaatgttaa attactgagt aatattaaag tgcaaaaagg tatgattata
      361 actatcgtct cactattatt actgacgtta gtaataggta aagatattaa tggttctaaa
      421 agttggataa acttaggatt tatgaactta caggcatctg agttattaaa aattgcaatc
      481 atattatata tcccatttat gatcagtaaa aaaatgccta gagtattaag taaaccaaaa
      541 ttaattttaa gtcctattgt attagcatta ggttgtacgt ttttagtttt cctacaaaaa
      601 gacgtagggc aaacattact aatattaatt attttagttg cgatcatttt ttattcagga
      661 attggggtaa acaaagtcct aagatttggt ataccagcag tactaggatt cttagtagta
      721 tttgtcattg cattaatggc tggttggtta ccaagttatt taactgccag atttagtacg
      781 ctaacagatc cattccaatt cgaatcagga actggatacc atatttccaa ttcattgctt
      841 gcgataggta acggtggcgt atttggaaaa ggattaggaa atagtgcaat gaaattgggc
      901 tatttaccag aaccacatac agattttatt tttgcaatta tttgcgaaga attaggttta
      961 atcggaggat tgctagttat tactttagag ttctttattg tatatcgtgc cttccagttt
     1021 gcaaataaaa catcatcata tttttataaa cttgtgtgtg ttgggattgc cacatacttt
     1081 ggaagtcaaa cgtttgtaaa cattggcggt atttcggcaa caattccatt aactggtgtg
     1141 ccattgccat ttatcagctt tggtggatca tcaatgatta gtttaagtat tgctatggga
     1201 ttacttctga ttgtaggtaa acaaatcaaa gtagaccagc aacgaaagaa acaacaacaa
     1261 aaagttgata taagaagaca atttaattaa aaaaatacta gccaatattt agtacgagat
     1321 gacttctgtc attaagcaat gctaaacatt ggctatttat atactctttg tcaaattgga
     1381 ctgatgagtt caataacgga agttaggcaa ccaaacaacg cttaacttcc tttttgtttt
     1441 gatattacga gattactcat gattatcaaa ttttataaac aaaacaacgt ataattataa
     1501 ctaaatttta tgactaaaaa ttactaaatg tggtggcgaa attagaatca tgatgataag
     1561 ataaagttat cgctattgat aataaatgtt gatgttttgc aattttattt ttaatttttt
     1621 agagttgtag aaatcgaaaa tgtttcacaa caaaaacgtg aactacctgt tgttgcatag
     1681 tcatttttga aattattatt tctgaagcgg aggttttaat tgtctcaaga atgttaaatt
     1741 ttgaaaattt gaaacataac tatagcaaaa cagagcgctt taagataaaa ttttattatc
     1801 taattagtta gaaaattcta atagtttgag gagtgtatcg ctattgaaac aaataaaaaa
     1861 gttacttgtt gctaaccgtg gagaaattgc aattcgtata ttcagagcgg cggcagaatt
     1921 agacatcagc acagttgcaa tttattcgaa tgaagacaaa agttcattac atagatataa
     1981 agcagatgaa tcctatttag ttggaagtga tttaggtcct gctgaaagtt atttaaatat
     2041 tgagcgtatc attgatgtag caaaacaagc gaatgtggat gcgattcatc ctggctatgg
     2101 atttttaagt gaaaatgaac aatttgcgcg tcgttgtgca gaagaaggaa ttaaatttat
     2161 tggtcctcat ttagaacatt tagatatgtt tggagataaa gttaaagctc gtacaacggc
     2221 tatcaaggca gatttaccag ttattcctgg tacagacggt ccaattaaat catatgaatt
     2281 agcaaaagaa tttgcagaag aagctggttt cccgctaatg attaaggcca caagtggtgg
     2341 cggcggtaaa ggtatgagaa tcgttcgtga agaaagtgaa ttagaagatg ctttccatag
     2401 agcaaaatca gaagctgaaa aatcatttgg taatagtgaa gtttacatag agagatacat
     2461 tgataatcca aagcatattg aagtacaagt tataggtgac gaatatggaa atatcgtaca
     2521 cttatttgaa cgtgattgtt cagtacaacg tcgtcatcaa aaagttgtag aagttgcacc
     2581 atcagttgga ttatcaccaa cattacgtca acgtatttgt gatgctgcaa ttcaattgat
     2641 ggaaaatatt aaatatgtca atgcaggtac tgttgaattt ctagtatctg gtgacgaatt
     2701 cttctttata gaagttaacc ctcgtgtaca agttgagcat acaattacag agatggtaac
     2761 aggaattgat attgttaaga cacaaatttt agttgcagca ggtgccgatt tatttggtga
     2821 agaaattaat atgccgcaac aaaaagatat tacaacattg ggctatgcca tccaatgtcg
     2881 tattacaaca gaagatccgt taaatgattt catgccggat actggaacaa tcattgctta
     2941 tcgttcaagc ggtggctttg gtgtacgtct cgatgctgga gatggtttcc aaggtgctga
     3001 gatatcacct tattatgatt cattactcgt aaaattatct acacacgcga tatcatttaa
     3061 acaagcagaa gaaaaaatgg tacgttcatt acgagaaatg cgtattcgtg gtgttaaaac
     3121 taatattcca ttcttaatta atgtaatgaa gaataaaaag ttcacaagtg gtgattacac
     3181 aactaaattt attgaagaaa caccagaact tttcgacatt cagccgtctc tagatagagg
     3241 tactaaaaca ttagaatata taggtaatgt aacaattaat ggtttcccaa atgttgagaa
     3301 acgtccgaaa ccagactatg aattagcatc aattccaact gtatcttcaa gtaaaatcgc
     3361 ttcatttagt ggtacgaaac aattgcttga tgaagtaggt ccaaaaggtg tagctgaatg
     3421 ggttaaaaag caggatgatg ttttactaac agatacaacc tttagagatg cacaccaatc
     3481 attattagct acacgagtta gaactaagga tatgattaat atcgcatcca aaacagcgga
     3541 cgtatttaaa gatggtttct cacttgaaat gtggggcggt gctacatttg atgtggcata
     3601 taatttcttg aaggaaaacc catgggaacg acttgaacgt ctacgtaaag ctattccaaa
     3661 tgtattattc caaatgttgt tacgtgcttc aaacgcagtt ggttataaaa actatcctga
     3721 taatgttatt cataaattcg tacaagaaag tgctaaagca ggcatagatg tctttagaat
     3781 tttcgattct ttaaactggg tagatcaaat gaaagttgcc aatgaagcag tacaagaagc
     3841 gggcaaaatc tctgaaggta ctatttgtta tacaggtgac attttaaatc ctgagcgatc
     3901 aaacatttat actttagagt attatgtcaa actagctaaa gagttagaac gtgaaggttt
     3961 ccatatttta gcgattaaag atatggcagg cttattaaaa cctaaagccg cttacgaatt
     4021 gattggtgag ttaaaatcag ctgtagattt accaattcat cttcacactc atgatacaag
     4081 tggtaatggt ttattaacat acaaacaagc aatagatgct ggtgtcgata tcattgatac
     4141 tgctgttgct tcaatgagtg gtttaacaag tcagccaagc gccaattcgt tatattatgc
     4201 attaaatggc ttcccacgcc accttagaac tgatattgaa ggtatggagt cacttagtca
     4261 ttattggtca actgtacgta cttattattc agactttgaa agtgatatca aatcaccgaa
     4321 tactgaaatt tatcaacatg aaatgcctgg tggacagtat tcgaatttaa gtcaacaagc
     4381 taaaagttta ggtttaggcg aaagatttga tgaagtcaaa gatatgtatc gcagagtgaa
     4441 tttcttattt ggtgatatcg taaaagtaac tccatcgtct aaagtagttg gtgatatggc
     4501 actttatatg gtacaaaatg atcttgatga acaatccgtg attacagatg gctataaatt
     4561 agatttccca gaatcagtag tgtcgttctt caaaggcgaa ataggacaac ctgtaaatgg
     4621 ttttaataaa gatttacaag cggttatttt aaaaggccaa gaagcactaa cagctcgtcc
     4681 aggtgaatat ctagagccag ttgattttga aaaagtccgt gagttgcttg aagaagagca
     4741 acaaggtcct gttacggagc aagatattat tagttatgta ttatatccaa aagtatatga
     4801 acaatatatt caaactagaa atcaatacgg aaacttatcg ttacttgata cgcctacatt
     4861 cttctttgga atgcgtaatg gtgaaacagt agaaatcgaa atcgataagg gtaaacgatt
     4921 aattattaaa ctagaaacga ttagtgaacc agatgaaaat ggtaatagaa cgatttacta
     4981 tgcgatgaat ggtcaagcga gacgtattta cattaaagat gagaatgtgc atacaaatgc
     5041 gaacgttaag ccaaaagcag ataagagtaa tccaagtcat atcggtgcgc aaatgccagg
     5101 ttcagtaact gaagtcaagg ttagtgtagg tgaatctgtg aaagctaacc agccgttgct
     5161 aattactgaa gctatgaaaa tggaaacaac aattcaagca ccatttgacg gtgtgattaa
     5221 acaagtaact gtaaataatg gtgacacaat agcgacaggc gatttattaa tcgaaattga
     5281 aaaagcaact gactaaaatg attaaaataa aacgagatta cacaacaatt gacgtgtaat
     5341 ctcgttttta catggcttag gtttaatttt aaatattgat ataaaaaacc gtaattttaa
     5401 tgggcgtgcc attaaaatta cggtttgctt ttttgttatt gcttgtcact tcttactgat
     5461 cgtaacatta gcatgataaa gtatgttgtc ataccaaata aataagtgat aaataatgca
     5521 tggaaaagtg cgattagcag gttaacattt gtcataatag ataatgcacc tgtgataact
     5581 tgtaatataa caagtataaa tgcagctgta taaccataat gaacagtacg gttatttgga
     5641 taatttttaa cagcgtgaat ataagtaatc ataataatcg taaacactat aaaagccatg
     5701 atacgatgcg tgagttgaac ccaatcttgt tctgaatgtg gtacaagatc gtggaatggc
     5761 aatggccaac caccatatgc taaacttgca tccgcatgtc tcactagtgc accagtataa
     5821 acaccacaat aaatgatgat tgccattaac catgttaaac gtcttaatgg ctttttgata
     5881 tataattcgt cagcttcata tttttgatct atagagaaaa taatcaatgt tattaaaaat
     5941 acagatgaga aactgattaa tgatatacca aagtgcaatg ctaaaacgta atcgttttgt
     6001 tgccaaataa cagcagcagc tccgattaat gcttgcaata ataagaatcc aacactaatg
     6061 attgataaag gtttaatttc tttaatatag cctatatgtt tccatgcagt gataactaac
     6121 cataagacca ttaataaaga caaagctgaa acggctctat gacttaactc aataatcgta
     6181 tcaataggaa agaattctgg aatcaacgca ccatgacata gtggccaaga agaaccacaa
     6241 ccatcagctg atccggtttt ggtaactaag gctccaccaa gttgtacaaa tgtcatcatt
     6301 aacgttgcta cgacacctaa ccattttaaa ttctttttgc caaacaattt atacacccca
     6361 tcataaaaaa acgcatcact gagaattaga attaatagcc ggatttgaga tgatactata
     6421 aattgacctg attgtaggat atgaaaagat gggatccaac gcaggaaata tgatttaatt
     6481 ttacaagtat tgtatgtgga cgacataaaa agaacttaat cgtaatgatt tttttattgg
     6541 gat
//
LOCUS       DAMZKC010000134         6528 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.136, whole genome shotgun sequence.
ACCESSION   DAMZKC010000134 DAMZKC010000000
VERSION     DAMZKC010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6528
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.136"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            118..1566
                     /locus_tag="POB55_002061"
     CDS             118..1566
                     /locus_tag="POB55_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDI6501016.1"
                     /translation="MGSFFNKIARKEDPAIYQNKDGHLKRTLRVRDFLALGVGTIVST
                     SIFTLPGIVAAEHAGPAVALSFLLAAIVAGLVAFTYAEMAAAMPFAGSAYSWVNVLFG
                     EFFGWVAGWALLAEYFIAVAFVASGFSANLRGLVKPIGIELPAALSNPFGTNGGFIDI
                     IAAIVILLTALLLSRGMSEAARMENILVILKVLAIILFVIVGLTAINVSNYVPFIPEH
                     KVTATGDFGGWQGIYAGVSMIFLAYIGFDSIAANSAEALDPQKTMPRGILGSLSVAIV
                     LFIAVALVLVGMFHYSQYANNAEPVGWALRQSGHGVVAAIVQAISVIGMFTALIGMML
                     AGSRLLYSFGRDGLLPSWLSHLNDKHLPNRALVILTIIGVLIGSMFPFAFLAQLISAG
                     TLVAFMFVSLAMYRLRKREGKDLPIPAFKLPLYPVLPAVTFVLVLLVFWGLGFEAKLY
                     TLIWFIVGIILYLSYGLRHSKKNDVAEYHPPK"
     gene            complement(1646..2983)
                     /locus_tag="POB55_002062"
     CDS             complement(1646..2983)
                     /locus_tag="POB55_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HDI6501017.1"
                     /translation="MSKAHQLIQEDEHYFAKSGRIKYYPLVIDHGYGATLVDIEGKTY
                     IDLLSSASSQNVGHAPREVTEAIKAQVDKFIHYTPAYMYHEPLVRLAKKLCEITPGDF
                     EKRVTFDLTGSDANDGIIKFARAYTGRPYIISFTNAYHGSTFGSLSMSAISLNMRKHY
                     GPLLNGFYHIPFPDKYRGMYEQPQANSVEEYLAPLKEMFAKYVPADEVACIVIETIQG
                     DGGLLEPVPGYFEALEKICREHGILIAVDDIQQGFGRTGTWSSVSHFNFTPDLITFGK
                     SLAGGMPMSAIVGRKEIMNCLEAPAHLFTTGANPVSCEAALATIQMIEDQSLLQASAE
                     KGEYVRKRMDQWVSKYNSVGDVRGKGLSIGIDIVSDKKLKTRDGSAALKICNYCFEHG
                     VVIIAVAGNVLRFQPPLVITYEQLDTALNTIEDALTALEAGNLDQYDISGQGW"
     gene            3241..3657
                     /locus_tag="POB55_002063"
     CDS             3241..3657
                     /locus_tag="POB55_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501018.1"
                     /translation="MRLKVLFHFIAAIFISFMLLWMTMLFDLISNQSHLKALLLNLDF
                     LIPSDNTPFILEIICHLLIGSLIYFVFVLLFHTSKRLYYLCYIPLFFLFIALYPFLVF
                     IAQRPIFQFSVTELIGWIITHIFFMSLMALVIPRIK"
     gene            3780..4670
                     /locus_tag="POB55_002064"
     CDS             3780..4670
                     /locus_tag="POB55_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002478556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose bisphosphate aldolase"
                     /protein_id="HDI6501019.1"
                     /translation="MNKEQLEKMKNGKGFIAALDQSGGSTPKALKEYGVNEDQYSNED
                     EMFQLVHDMRTRVVTSPSFSPDKILGAILFEQTMDREVEGKYTADYLADKGVVPFLKV
                     DKGLAEEQNGVQLMKPIDNLDSLLDRANERHIFGTKMRSNILELNEQGIKDVVEQQFE
                     VAKQIIAKGLVPIIEPEVNINAKDKAEIEKVLKAELKKGLDNLNADQLVMLKLTIPTE
                     PNLYKELAEHPNVVRVVVLSGGYSREKANELLKDNAELIASFSRALASDLRAGQSKEE
                     FDKALGDAVESIYDASVNKN"
     gene            complement(4864..6360)
                     /gene="mqo"
                     /locus_tag="POB55_002065"
     CDS             complement(4864..6360)
                     /gene="mqo"
                     /locus_tag="POB55_002065"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HDI6501020.1"
                     /translation="MAKSNSKDIVLIGAGVLSTTFGSMLKEIEPDWNIHVYERLDRPA
                     IESSNERNNAGTGHAALCELNYTVLQPDGSIDIEKAKVINEEFEISKQFWGHLVKSGS
                     IENPREFINPLPHISYVRGKNNVKFLKDRYEAMKAFPMFDNIEYTEDIEVMKKWIPLM
                     MKGREDNPGIMAASKIDEGTDVNFGELTRKMAKSIEAHPNATVQFNHEVVDFEQLSNG
                     QWEVTVKNRLTGEKFKQVTDYVFIGAGGGAIPLLQKTGIPESKHLGGFPISGQFLACT
                     NPQVIEQHDAKVYGKEPPGTPPMTVPHLDTRYIDGQRTLLFGPFANVGPKFLKNGSNL
                     DLFKSVKTYNITTLLAAAVKNLPLIKYSFDQVIMTKEGCMNHLRTFYPEARNEDWQLY
                     TAGKRVQVIKDTPEHGKGFIQFGTEVVNSQDHTVIALLGESPGASTSVSVALEVLERN
                     FPEYKTEWAPKIKKMIPSYGESLIEDEKLMRKIRKQTSKDLELGYYEN"
BASE COUNT         1985 a         1161 c         1110 g         2272 t
ORIGIN      
        1 aacataaagt tgtgattttt cagaattttt agacaaaata ttgattattt gtgctatgat
       61 ataacatatt aaaaaacacc gtatagaatt gtttatgaat gtttgggagg aaagtttatg
      121 ggaagttttt tcaataaaat agcacgaaaa gaggatccgg ctatctatca aaataaagat
      181 ggtcatttaa agcgtacact tcgggtgcgt gatttcttag ctttaggtgt aggaacaatt
      241 gtatcgacat ctatctttac actacctggc attgttgctg cagaacatgc aggaccggcc
      301 gttgcgttat cattcttact cgctgctatt gttgctggtt tagttgcatt tacttatgca
      361 gaaatggctg ccgctatgcc atttgcaggt tcagcctatt cttgggtcaa tgtattattt
      421 ggtgaatttt ttggatgggt tgccggttgg gctctattag ctgaatattt tatcgccgta
      481 gcctttgttg catcaggatt ctcagcgaat ttacgcggac ttgtgaaacc aattggcatc
      541 gaattaccag cagcattatc aaatccattt ggtacgaatg gcggttttat cgatattatt
      601 gctgctatcg ttattttatt aactgcatta ttactatcac gtggtatgtc ggaagcagca
      661 cgtatggaaa atattttagt tattttaaaa gtattagcta ttattttatt tgtcatcgta
      721 ggtttaacag caataaatgt tagtaactat gtgccattta ttccagaaca caaagtaact
      781 gctacaggtg actttggtgg ttggcaaggc atatatgctg gtgtttcaat gattttctta
      841 gcgtatatcg gtttcgattc tatcgcagca aactcagcag aagcacttga ccctcaaaag
      901 acaatgccta gaggtattct tggttcttta agtgttgcta tcgtattatt tattgctgta
      961 gcacttgtgt tagttggtat gttccattac tcacaatacg caaacaatgc tgaacctgtt
     1021 ggttgggctt tacgtcaaag tggtcatggt gttgtagcag ctattgttca agctatctct
     1081 gttatcggta tgtttacagc attaattggt atgatgttag caggctcacg tttactttat
     1141 tcatttggac gtgacggctt attaccttca tggttaagcc atttaaacga caaacattta
     1201 cctaatcgcg cactagttat acttactatt attggtgttt taattggttc tatgttccca
     1261 ttcgcatttt tagcacaatt aatttcagca ggtacacttg ttgcatttat gttcgtttca
     1321 ttagcaatgt atcgtttgag aaagcgtgaa ggtaaagatc taccaattcc tgcatttaaa
     1381 ttacctttat atcctgtgtt accagcagtt acatttgttt tagtattgct agtattctgg
     1441 ggattaggat tcgaagcgaa attatacact ttaatttggt ttattgttgg tatcattcta
     1501 tatttaagtt atggacttag acattctaag aaaaatgacg tagctgaata ccacccacca
     1561 aaataaaaat caaaaaaatc atttcccatc tgattaacat ccagatgtgg aaatgatttt
     1621 ttattttata ttaagataat cgctattacc aaccttgtcc agatatgtca tattgatcta
     1681 agttacctgc ttccaaagca gtcagtgcat cttctatagt gtttaacgcc gtgtctaatt
     1741 gctcatatgt tattaccaat ggcggttgga atcgcaacac atttcctgct acagctataa
     1801 taactacgcc atgctcaaag cagtaattac aaattttaag tgccgcactg ccatcacgtg
     1861 ttttgagttt tttgtcggaa acaatatcaa taccaatgct caaaccttta cctctaacat
     1921 cgcctacact attgtattta gatacccatt gatccattcg tttcctaaca tattcccctt
     1981 tttccgcact agcctgaaga agcgactgat cttcaatcat ttgaattgtg gctaatgcag
     2041 cttcacaact aactggatta gcacctgttg taaataaatg tgctggcgct tctaaacaat
     2101 tcataatctc tttgcgtcca acaattgctg acataggcat accacctgct aaagattttc
     2161 caaaagtgat taaatcaggc gtaaaattaa aatgcgagac tgaactccat gtacctgttc
     2221 gcccaaaacc ttgttgaata tcatcgacag cgattaaaat accatgttca cgacaaatct
     2281 tttctaacgc ttcaaaatac cctggaactg gttctaaaag tccaccatcg ccttgtatcg
     2341 tttcaataac aatacatgct acttcgtcag caggtacata tttcgcaaac atttctttta
     2401 agggtgctaa atattcttct actgaattag cttgtggctg ctcgtacata ccacgatatt
     2461 tatctggaaa cggaatatga taaaaaccat tcaataacgg accataatgt ttgcgcatat
     2521 ttaaactaat agctgacata gacaatgagc caaaagttga accatgatat gcattagtga
     2581 aactaatgat ataaggacgc cctgtatatg ctctggcaaa tttaatgatg ccatcatttg
     2641 cgtctgatcc ggttaaatcg aaggtcactc ttttttcaaa atctccaggt gtaatctcac
     2701 aaagcttctt agctaaacgt accaacggtt catgatacat atatgctggt gtataatgaa
     2761 taaatttatc aacttgtgcc tttatcgctt ctgttacttc tctaggtgca tgacctacgt
     2821 tttgagaact cgcactcgat aacaaatcga tatatgtctt cccctcaata tcaaccaatg
     2881 ttgctccata tccatgatca atcactaacg gataatattt aatacgtcct gatttcgcaa
     2941 aataatgttc atcctcttga attaattgat gtgctttact catatacatt cccctcccat
     3001 tgacagtatt aatgtgcaag caaagtggcc tctcttaaat cgattataaa atccgattta
     3061 aaatactgct caaataccgt catgcctcta tgaatactat ccaatatact aataaatttc
     3121 agatttgtaa atatattctg ataattaaga attttatatc aatctacttt gtttatgcgc
     3181 ataattttgt tcgattaggg aattgtttac tatattccat ttgactctga ggtgatacga
     3241 atgcgtttaa aagtattgtt ccatttcatt gctgctattt tcatatcatt catgttgcta
     3301 tggatgacca tgttgtttga cttaataagc aatcaatcac atttaaaagc tttattattg
     3361 aatttagatt ttctaattcc ttctgacaat acaccattta ttctagaaat aatatgtcac
     3421 ttactaattg gtagcctcat ttattttgta tttgtgttac tgtttcatac ttctaaaagg
     3481 ctttactatc tatgttatat ccctttattt ttcttattta tagcattgta tcccttttta
     3541 gtatttattg cgcaacgtcc aatttttcaa tttagtgtga cagaattaat cggttggatt
     3601 attacacata ttttctttat gtcattaatg gcgttagtca tacctagaat aaaataatat
     3661 ttttaattaa actgacaatt atttttcaca ttttatacac ttttcaaggt ttatgacttg
     3721 agaaatgttc tcatttttat catactggag gtgtattgaa aagttaagga gtggaaagta
     3781 tgaataaaga gcaattagaa aaaatgaaaa atggaaaagg ctttattgcc gcattagacc
     3841 aaagtggtgg tagtacacca aaagcactta aagaatatgg tgtaaacgaa gatcaatata
     3901 gcaatgaaga cgaaatgttc caacttgttc acgatatgcg tacacgtgtg gtaacttcac
     3961 cttcattctc accagataaa attttgggtg ctattctttt cgaacaaaca atggatcgcg
     4021 aagtagaagg caaatacact gcagattact tagctgataa aggtgttgtt ccgttcttaa
     4081 aagtagacaa aggtcttgct gaagagcaaa atggtgttca attaatgaaa ccaatcgaca
     4141 acttagacag tttattagac cgtgcaaacg aacgtcacat ttttggtaca aaaatgcgtt
     4201 ctaacatttt agaattaaat gagcaaggta tcaaagacgt tgttgaacaa caatttgaag
     4261 ttgctaaaca aattattgct aaaggtttag ttccaattat cgaaccagaa gttaatatta
     4321 atgcaaaaga caaagctgaa attgaaaaag tattaaaagc tgaacttaaa aaaggtttag
     4381 ataacttaaa tgctgatcaa ttagtaatgt tgaaattaac aattcctact gaaccaaact
     4441 tatacaaaga gttagctgaa catcctaatg ttgttcgtgt cgttgtatta tcaggtggtt
     4501 acagcagaga aaaagcaaat gaattactta aagataacgc tgaacttatc gcaagcttct
     4561 cacgtgcatt agctagtgat ttaagagctg gacaatctaa agaagaattc gataaagcat
     4621 taggtgacgc tgtagaatca atctacgacg catctgtaaa caaaaactaa tttatacatt
     4681 aaacatattt tttgatataa actactaaaa ggactcatac atgtgcttta aaggcattgt
     4741 gtatgagtct ttttgattaa agttgatttt gccatatgta ttatagatta cgcgaaaaaa
     4801 tcggacagct gaaaaatcta taagatttct cagttgcccg attttaaata tagctattag
     4861 aatttagttt tcgtagtaac ctaattctaa gtcttttgaa gtttgtttac ggatttttct
     4921 cattaatttt tcgtcttcaa ttaatgattc accgtatgat ggaatcattt tcttgatttt
     4981 aggtgcccat tcagttttgt attcagggaa gttacgttct aatacttcta acgcaactga
     5041 aactgaagtt gaagcccctg gtgattcacc taataatgcg attacagtgt ggtcttgtga
     5101 gttaaccact tctgtaccga attggatgaa tcctttaccg tgttcaggtg tatctttgat
     5161 aacttgtaca cgtttaccag cagtgtataa ttgccaatct tcattacgtg cttctggata
     5221 gaaagtacgt aagtggttca tacaaccttc ttttgtcata attacttggt caaatgagta
     5281 tttaattaaa ggtaagtttt taactgctgc tgctaataaa gttgtaatgt tgtatgtttt
     5341 aacagacttg aataaatcta agttagaacc atttttcaag aatttaggtc caacattagc
     5401 aaatggtcca aataacaatg ttctttggcc gtcaatgtaa cgcgtatcta aatgaggtac
     5461 agtcattggt ggtgtaccag gtggctcttt accataaact ttggcatcgt gttgttcaat
     5521 aacttgtggg tttgtacaag ctaagaattg accactgata gggaatccac ccaaatgttt
     5581 actttcaggg atacctgttt tttgtaataa tggaatcgct ccaccgccag caccgatgaa
     5641 tacatagtca gttacttgtt tgaatttctc accagttagg cgatttttaa cagtaacttc
     5701 ccattgacca tttgataatt gttcaaaatc aacaacttca tggttaaatt gcactgtagc
     5761 atttggatgt gcttcaatgc ttttagccat tttacgtgtt aattcaccga agtttacatc
     5821 tgtaccttcg tcaattttac ttgccgccat gataccaggg ttatcttcac ggcctttcat
     5881 catcaatgga atccattttt tcattacttc gatgtcttca gtatattcga tattatcgaa
     5941 catagggaaa gctttcatcg cttcgtaacg atcttttaag aatttaacat tgtttttacc
     6001 tctaacataa ctgatgtgtg gtaatggatt gataaattct cttgggttct cgatgctacc
     6061 gcttttcact aagtgacccc agaattgttt tgaaatctca aactcttcgt taatcacttt
     6121 cgctttttcg atgtcgatag aaccatcagg ttgtaaaact gtgtagttca actcacataa
     6181 tgctgcatga cccgtaccag cattatttct ttcgtttgaa ctttcgattg caggacgatc
     6241 caagcgttcg taaacgtgga tattccagtc tggctcaatt tcttttaaca ttgaaccaaa
     6301 tgttgtgcta agtacaccgg ctccaattaa aacgatgtct ttactattag acttagccat
     6361 tggtttcacc tctccaaaaa ttgtaaatgt gtatcatcaa tatgaaagtt acataaaact
     6421 gacatatttc tttaaaatat caacgccata gataacttcc tgttttaatt gatacgctgt
     6481 aacaaaatac tatagttagt gcttacatgt atatgttaaa gcaagcag
//
LOCUS       DAMZKC010000135         6496 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.137, whole genome shotgun sequence.
ACCESSION   DAMZKC010000135 DAMZKC010000000
VERSION     DAMZKC010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6496
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.137"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            122..622
                     /gene="ftnA"
                     /locus_tag="POB55_002066"
     CDS             122..622
                     /gene="ftnA"
                     /locus_tag="POB55_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-type ferritin FtnA"
                     /protein_id="HDI6501021.1"
                     /translation="MLSKNLLEALNDQMNHEYFAAHAYMAMAAYCDKESYEGFANFFI
                     QQAKEERFHGQKIYNYINDRGAHAEFRAVSAPKIDFSSILETFKDSLSQEQEVTRRFY
                     NLSEIARQDKDYATISFLNWFLDEQVEEESMFETHINYLTRIGDDSNALYLYEKELGA
                     RTFDEE"
     gene            complement(704..796)
                     /locus_tag="POB55_002067"
     CDS             complement(704..796)
                     /locus_tag="POB55_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501022.1"
                     /translation="MTKNTIISLENEKTQINDSENESSDLRKAK"
     gene            1012..1566
                     /locus_tag="POB55_002068"
     CDS             1012..1566
                     /locus_tag="POB55_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HDI6501023.1"
                     /translation="MIQDAFVALDFETANGKRTSICSVGMVKVIDSQITETFHTLVNP
                     QDYFSQQNIKVHGIQPEDVENAPTFDYVFPYMMQFIADLPVVAHNAAFDMNVLHQSIQ
                     NIGLPTPNLTYFCSYQLAKRTVDSYRYGLKHMMEFYQLDFHGHHDALNDAKACAMITF
                     RLLKNYENLTYVTNIYGKNLKDKG"
     gene            complement(1633..2703)
                     /gene="dinB"
                     /locus_tag="POB55_002069"
     CDS             complement(1633..2703)
                     /gene="dinB"
                     /locus_tag="POB55_002069"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDI6501024.1"
                     /translation="MTERRIIHIDMDYFFAQVEMRDNPKLKGKPVIVGGKASSRGVVS
                     TASYEARKYGVHSAMPMSQAHKLCPNGYFVTSNFGAYRETSAQIMSIFRSYTDKVEPM
                     SLDEAYLDITELVRPDLPASKIAQYIRKDILEQTHLTASAGVSYNKFLAKLASGMNKP
                     DGMTVIDYQNVHDILMTLDIGDFPGVGKASKKVMHDNGIFNGRDLYEKTEFELIRLFG
                     KRGRGLYNKARGIDHSEVKSSRVRKSVGTERTFATDVNDDEEILRKVWELSGKTAERL
                     NKLQKSAKTVTVKIKTYQFETLSKQMSLRDSVSSEEDIYNIAYLLYNDLKDPDVPIRL
                     IGVTVGNLEQSTYKNMTIYDFI"
     gene            complement(2950..3480)
                     /locus_tag="POB55_002070"
     CDS             complement(2950..3480)
                     /locus_tag="POB55_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HDI6501025.1"
                     /translation="MHKIDLTTNNFQMRRFIILQLVIALFVILFTYKWALGVTAVVDQ
                     NIIINLVYGFAGFIILLILHELIHRALFLLFKKDSKPMFNIKKDRILFQTADACFNKW
                     QFSIIMLSPLILLSTGLLILIKVFGYSSLIFMFSMHTAYCFIDILLVALTISSSFKYV
                     QQDEDSIYLYHQKPAQ"
     gene            complement(3650..5011)
                     /gene="rlmD"
                     /locus_tag="POB55_002071"
     CDS             complement(3650..5011)
                     /gene="rlmD"
                     /locus_tag="POB55_002071"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDI6501026.1"
                     /translation="MQAIAKNDIKTGTVVDLTHEGHGVVKIDRFPIFIPQALINEQIE
                     YKIIKVKKNFAIGKLLNINTRSENRVAPPCIYYERCGGCQLQHLSYEAQLEMKKEQVI
                     NLFQRKAHFDNSKINDTVGMTDPWRYRNKSQIPVGKNEQNEVIMGFYRQRSHDIIDME
                     SCLIQDSQHQEVMNEVKSILKDLNVSIYQEQLKKGLMRHLVVRTGYHTDEMMIIFVTN
                     GKKWPQKNAVVEKILDAFPNVTSIKQNINDSHSNVIMGRQSITLYGKDTIIDQLTDST
                     FKISDQSFYQINSEQTEKLYNKAIEYAQLTGNEVVLDTYCGIGTIGLYMAPHAKHVYG
                     VEVVPSAIEDAQQNATINQCNNTTFVCGKAEEVILQWKAQGIKPDVVMVDPPRKGCDE
                     TFIQTLLTLEPKRIVYISCNPATQQRDALLLAEKYQLEEVTPVDMFPQTTHVETVALF
                     NLK"
     gene            complement(5092..6039)
                     /locus_tag="POB55_002072"
     CDS             complement(5092..6039)
                     /locus_tag="POB55_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDI6501027.1"
                     /translation="MRKRARIIYNPTSGKELFKRELPDALIKLEKAGYETSAYATEKI
                     GDATLEAERAMHENYDVLIAAGGDGTLNEVVNGIAEKPNRPKLGVIPMGTVNDFGRAL
                     HIPNDIMGALDVIIEGHSTKVDIGKMNNRYFINLAAGGQLTQVSYETPSKLKSIVGPF
                     AYYIKGFEMLPQMKAVDLRIEYDGNVFQGEALLFFLGLTNSMAGFEKLVPDAKLDDGY
                     FTLIIVEKSNLAELGHIMTLASRGEHTKHPKVIYEKAKAINISSFTDLQLNVDGEYGG
                     KLPANFLNLERHIDVFAPNDIVNEELINNDHVDDNLIEE"
BASE COUNT         2120 a         1158 c          913 g         2305 t
ORIGIN      
        1 caattagagt gctaaaataa ggtgttaaat tataattatt attatttaca attgagaatg
       61 agtttgttat actaagttta attcatagta attttaattt acaaataaga ggtgtatcaa
      121 aatgttaagt aaaaatttat tagaagcatt aaatgatcaa atgaaccatg agtactttgc
      181 agcacacgca tatatggcaa tggcagcata ctgtgataaa gaatcgtacg aaggatttgc
      241 aaacttcttc attcaacaag ctaaagaaga acgtttccat ggacaaaaga tttataacta
      301 tattaacgac agaggtgcac atgcagaatt cagagcagtt tcagcaccaa aaattgactt
      361 ttcaagcata ctagaaactt tcaaagacag cttatctcaa gaacaagaag taacaagacg
      421 tttctataac ttatctgaaa tcgctcgtca agataaagat tatgcaacta tctcattctt
      481 aaactggttc ttagatgaac aagtcgaaga agaatcaatg tttgaaactc acatcaatta
      541 tttaactcgt atcggcgatg acagcaatgc attatatctt tacgaaaaag aacttggcgc
      601 tcgtacattc gacgaagaat aattaaacat cactacaata gacagataaa tatcatacga
      661 catgataggc atttgggtca cttacaataa cccaatgtct atattatttt gctttacgga
      721 gatcactaga ttcattttct gaatcattga tctgcgtttt ttcattttca aggctaatta
      781 ttgtattttt agtcatttat tttttaaact actaatgtta ataactctaa atttgatgtt
      841 gaattaattt gaccgatttt aaagcatatc atcatttact ttttaatcag agttacatcc
      901 aaatgataga tttcacgtta taccttcacg tataatatta tgtatcgttt gtaagcaaat
      961 gactaaaagt ctattaatat atacatttaa ttaattgaaa ggattgacta catgatacaa
     1021 gatgcgtttg ttgcacttga ttttgaaaca gcaaatggta aacgtacaag tatttgttct
     1081 gtcggaatgg ttaaagtcat tgatagtcaa ataacagaaa catttcatac tcttgtgaat
     1141 ccgcaagact atttttcaca acaaaatatt aaagttcatg gcatacaacc agaagatgtt
     1201 gaaaatgcac ctacgtttga ctacgtattt ccatatatga tgcaatttat tgcagattta
     1261 cctgttgtcg cacataacgc ggcatttgat atgaacgtct tacatcaaag cattcaaaat
     1321 attggtttac caacaccaaa tttaacttac ttttgtagtt atcaacttgc taaaagaacc
     1381 gttgattcgt atcgatacgg tttaaaacat atgatggagt tttatcaatt agattttcat
     1441 ggtcatcatg atgcattgaa tgatgccaaa gcatgcgcaa tgattacttt taggctactg
     1501 aaaaattatg aaaatttaac atatgtaact aatatttatg gtaaaaatct aaaagataaa
     1561 ggctaggact aaataaaata ctcccttcaa agtaagcatt gtaaaaatgt aaactttgca
     1621 gggagcttta ttttatataa agtcatatat cgtcatattt ttataagttg attgttctaa
     1681 attacctaca gtgacaccaa taagtcgaat tggtacatca gggtctttta aatcgttata
     1741 aagtaaatat gcaatattat aaatatcttc ttcagaacta accgaatctc ttaaactcat
     1801 ctgtttagat agcgtttcaa attgataagt tttaatttta accgttacag ttttagctga
     1861 cttctgtaat ttatttagac gttcagctgt tttacctgac aattcccata cttttcttaa
     1921 aatctcttca tcatcattca cgtctgttgc aaatgtgcgt tcagtcccta ctgattttct
     1981 tactcttgat gatttcactt cactatggtc aataccgcgt gccttgttat ataaaccccg
     2041 acctcttttt ccaaacaaac gtattaattc aaattccgtt ttctcatata aatctctacc
     2101 gttaaaaata ccattatcat gcattacttt tttggaagct ttacctacgc ctggaaaatc
     2161 tccaatatcc aatgtcatca aaatatcatg gacattttga taatcaatca cagtcatacc
     2221 atcaggttta ttcataccac tcgctaattt agctaaaaat ttgttataag aaacacctgc
     2281 agatgctgtt aaatgtgtct gctctagaat atcttttcta atatactgag caattttcga
     2341 agcaggaagg tctggtctca ctaattctgt aatatctaaa tacgcttcat ccaatgacat
     2401 cggttctacc ttatctgtat aacttcggaa aatagacata atctgcgcag atgtttctcg
     2461 gtaagcacca aaattacttg tgacaaagta tccatttgga cataatttat gcgcttgtga
     2521 cataggcatt gctgaatgga cgccgtattt tcgtgcttca taagatgccg tagagacaac
     2581 acccctactg cttgctttac caccaacaat aactggtttc cctttcaatt tggggttgtc
     2641 tctcatttcg acttgtgcaa aaaaatagtc catatctata tgaataattc gtctctcagt
     2701 caagtgctca cctccctact aatttttact tttataacgc acaaaaatat ctcaacataa
     2761 ttatacgctg tgtacgattt ttttacataa atcttgcact tagcgataac tatattgaga
     2821 taactacaag ttgttataaa atcaattgct atttaagcat gatgatgaag acgattgagt
     2881 aagaaaacat aggtaatctg aaataattca agcaaattca ttttgttggt atcatcatat
     2941 taaaatttat tattgagccg gcttttgatg atacaaataa atactatctt catcttgttg
     3001 aacatattta aaactactag atattgttaa tgcaacaagc agaatatcta taaagcaata
     3061 agcagtatgc atactaaaca taaaaataag tgatgaataa ccaaatacct taattaaaat
     3121 aagcaagcca gtacttaata ggattagtgg tgacagcata ataattgaga attgccattt
     3181 gttgaagcaa gcatctgctg tttggaataa gattctgtct ttttttatat taaacatagg
     3241 tttgctatct tttttaaata aaagaaataa tgctctatgg ataagttcat gtaaaatcaa
     3301 taaaataatg aatccagcaa acccatatac aagattgatg atgatatttt gatcgacaac
     3361 cgctgtgaca cctaacgccc acttatacgt aaataaaatc acgaataacg caataacaag
     3421 ttgcaagata ataaaccttc gcatttgaaa attatttgtc gttaaatcaa ttttatgcat
     3481 taccaaccct cccgatcatg acattcttat tcttctttaa atatagtata caatgtcaca
     3541 tttaatttaa aaagttcata tcaagaaagt aaattggctg taataaaatt ttaatatacg
     3601 acttctttct tcacttatta aggcgaaatt ttatctcaaa tcatgtgcgc tatttcaaat
     3661 tgaataatgc cactgtctca acatgtgttg tttgtggaaa catatctacc ggtgttacct
     3721 cttcaagttg atatttttca gctaataata atgcatcacg ttgctgtgtt gcgggattac
     3781 atgaaatata gacaatacgc ttaggttcta atgtaagcaa agtctgaata aacgtttcgt
     3841 cacagccctt tcttggcgga tcaaccatta caacatctgg tttaatccct tgtgctttcc
     3901 attgtaaaat aacttcttca gctttcccac agacaaaagt tgtattattg cattggttta
     3961 tagtcgcatt ttgttgtgcg tcttcaattg cagaaggtac tacttcaaca ccgtatacat
     4021 gttttgcatg tggtgccata tatagcccta ttgttccaat accacaatag gtatctaata
     4081 caacttcatt acctgtcaat tgcgcatatt caattgcttt attatataat ttctctgttt
     4141 gttcagaatt aatttggtag aatgactgat cacttatttt aaatgtacta tctgttaatt
     4201 gatcaataat tgtatcttta ccatatagcg ttatagattg acgccccata ataacattag
     4261 agtggctatc attaatgttt tgtttaatgc ttgtcacatt aggaaatgca tctaatatct
     4321 tctcaacaac agcatttttt tgtggccact ttttaccatt agttacaaaa ataatcatca
     4381 tttcgtctgt atgatatcct gttcttacaa ccaaatgtct cattaaacct tttttcaatt
     4441 gttcttgata aatacttaca tttaaatctt ttaaaataga tttaacttca ttcatcactt
     4501 cttgatgttg tgaatcttgt attaaacaac tttccatgtc aataatgtca tggcttcttt
     4561 gacgataaaa gcccataata acttcattct gttcattctt accaactgga atctgggact
     4621 tgtttcgata tctccaagga tctgtcatgc caactgtatc gttaatctta gaattatcaa
     4681 aatgcgcttt tcgctgaaac aaattaatca cttgttcctt tttcatttca agttgtgctt
     4741 cgtatgataa gtgttgaagt tggcacccac cacaacgttc ataatatata catggcggtg
     4801 caacacgatt ctcacttctc gtatttatat taagcaactt acctatcgca aagttctttt
     4861 ttactttgat aattttatat tcaatttgtt cattaattaa agcttgtggt atgaaaatag
     4921 gaaagcgatc tatttttacg acaccatggc cttcatgcgt taaatcaaca actgttcccg
     4981 tttttatgtc atttttagct attgcttgca aaattttacc tccaaaatga acaggttagg
     5041 aacaaaatta tgcgcttcct aacctgccat tatatatttc actatttctg tttattcttc
     5101 gattaaattg tcatcaacat gatcattatt tattaactct tcatttacaa tatcattagg
     5161 tgcaaagaca tctatatgac gttctagatt taagaaattc gctggtaatt taccaccata
     5221 ttctccatct acatttagtt gtaagtctgt gaatgatgaa atattaattg cctttgcttt
     5281 ttcataaata actttaggat gcttagtatg ttctcctctt gaagctaaag tcataatatg
     5341 accaagttct gcaaggtttg atttttcaac tataattaac gtaaaatagc cgtcatctaa
     5401 cttagcgtcc ggcactaatt tttcaaatcc tgccattgaa tttgttaaac ctaaaaagaa
     5461 taataatgct tctccttgga aaacattacc atcatattca attcttaaat ctacagcttt
     5521 catttgaggt aacatttcga aacctttgat gtaataagca aatggaccaa caatagattt
     5581 caatttactc ggtgtttcat aagagacttg cgtcaattgt ccgcctgcag ctaaattaat
     5641 aaagtatcga ttattcattt taccaatatc tactttagta gaatgacctt caatgatgac
     5701 atcaagtgcc cccatgatgt cattaggtat atgcaatgca cgtccaaagt cattaacagt
     5761 acccatagga atgacaccta gcttaggacg attaggcttt tctgcgatac cattaactac
     5821 ttcatttaat gttccatcac cacctgcagc gattaataca tcataatttt catgcatagc
     5881 tctttctgct tcaagtgtgg catcacctat tttctcggtt gcatatgcac tcgtttcata
     5941 tcccgctttt tctaatttta ttaaggcatc aggtaattct cttttaaata gctctttacc
     6001 tgatgtcggg ttataaatga ttctagcacg tttcctcata tcttatccct ctacttaaaa
     6061 ttcatatatt ttaacatcat ctttgtttcg tctaataggg agtgggacag aaataatatt
     6121 taacaaaatt tatttcgttc taccccaact tgcacattat tgtaagctga cagaaagtca
     6181 gcttctttgt tggggcccca ctagaattga aaaaagcttg ttacaagcgt attttctttc
     6241 agtcaactac tgccaatata acattgtagt gcctaggaca ttgaatttat gacccaggct
     6301 cagtcttatt tcatcattct taatatcgtt aaagaccaac ttgtatctta aacaaatact
     6361 atctcaatat gtacaaagct tgttatttat tcagcatttt ttgccgttct tcattatata
     6421 gacttcgtca gttatgctat tttaccttta aaatgatgtt gtaaatataa tgttgtcttt
     6481 atacaaaaat gtttta
//
LOCUS       DAMZKC010000136         6456 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.138, whole genome shotgun sequence.
ACCESSION   DAMZKC010000136 DAMZKC010000000
VERSION     DAMZKC010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6456
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.138"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(<1..118)
                     /locus_tag="POB55_002073"
     CDS             complement(<1..118)
                     /locus_tag="POB55_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501028.1"
                     /translation="MFYFTLSKQNTHSEKLNFSLIANINKIDTKLFERLLKLW"
     gene            complement(632..1603)
                     /gene="nrdF"
                     /locus_tag="POB55_002074"
     CDS             complement(632..1603)
                     /gene="nrdF"
                     /locus_tag="POB55_002074"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HDI6501029.1"
                     /translation="MIAVNWNTQEDMTNMFWRQNISQMWVETEFKVSKDIASWKTLSE
                     AEQDTFKKALAGLTGLDTHQADDGMPLVMLHTTDLRKKAVYSFMAMMEQIHAKSYSHI
                     FTTLLPSSETNYLLDEWVLEEPHLKYKSDKIVANYHKLWGKEASIYDQYMARVTSVFL
                     ETFLFFSGFYYPLYLAGQGKMTTSGEIIRKILLDESIHGVFTGLDAQHLRNELSESEK
                     QKADQEMYKLLNDLYLNEESYTKMLYDDLGITEDVLNYVKYNGNKALSNLGFEPYFEE
                     REFNPIIENALDTTTKNHDFFSVKGDGYVLALNVEALQDDDFVFDNK"
     gene            complement(1721..3826)
                     /gene="nrdE"
                     /locus_tag="POB55_002075"
     CDS             complement(1721..3826)
                     /gene="nrdE"
                     /locus_tag="POB55_002075"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000855513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HDI6501030.1"
                     /translation="MKTMEEKKYNHIELNNEVTKRREDGFFSLEKDQEALVAYLEEVK
                     DKTIFFDTEIERLRYLVDNDFYFNVFDIYSEADLIEITDYAKSIPFNFASYMSASKFF
                     KDYALKTNDKSQYLEDYNQHVAIVALYLANGNKAQAKQFISAMVEQRYQPATPTFLNA
                     GRARRGELVSCFLLEVDDSLNSINFIDSTAKQLSKIGGGVAINLSKLRARGEAIKGIK
                     GVAKGVLPIAKSLEGGFSYADQLGQRPGAGAVYLNIFHYDVEEFLDTKKVNADEDLRL
                     STISTGLIVPSKFFDLAKEGKDFYMFAPHTVKEEYGVTLDDIDLEKYYDDMVANPNVE
                     KKKKNAREMLNLIAQTQLQSGYPYLMFKDNANRVHPNSNIGQIKMSNLCTEIFQLQET
                     SIINDYGIEDEIKRDISCNLGSLNIVNVMESGKFRDSVHSGMDALTVVSDVANIQNAP
                     GVRKANSELHSVGLGVMNLHGYLAKNKIGYESEEAKDFANIFFMMMNFYSIERSMEIA
                     KERGIKYQDFEKSDYANGKYFEFYTTQEFEPQFEKVRELFDGMAIPTSEDWKKLQQDV
                     EQYGLYHAYRLAIAPTQSISYVQNATSSVMPIVDQIERRTYGNAETFYPMPFLSPQTM
                     WYYKSAFNTDQMKLIDLIATIQTHIDQGISTILYVNSEISTRELARLYVYAHYKGLKS
                     LYYTRNKLLSVEECTSCSI"
     gene            complement(3789..4187)
                     /gene="nrdI"
                     /locus_tag="POB55_002076"
     CDS             complement(3789..4187)
                     /gene="nrdI"
                     /locus_tag="POB55_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HDI6501031.1"
                     /translation="MKIIYFSFTGNVRRFIKRTELENTLEITAENCMEPVHEPFIIVT
                     GTIGFGEVPEPVQSFLEVNHQYIRGVAASGNRNWGLNFAKAGRTISEEYNVPLLMKFE
                     LHGKNKDVIEFKNKVGNFNENHGREKVQSY"
     gene            4988..5854
                     /locus_tag="POB55_002077"
     CDS             4988..5854
                     /locus_tag="POB55_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001594836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDI6501032.1"
                     /translation="MNPKVKGIIAILISAIGFSFMSVFFRLAGDLPVFQKSLARNLVA
                     MFIPLFFIYKYHQPMFGRLSSQPLLITRSTLGLIGVLLNIYAIDHMVLSDADSLMKLN
                     PFWTILLSIVFLHEKVRKYQITAMIIAILGMLLIVKPEFSSSMIPSLAGLFSGIFAAS
                     AYTCVRALSTREAPYTIVFYFSLFSVIVLIPFTAYTYEPMSQMQILYLLGAGLAAAVG
                     QIGVTLAYSFAAAKDISIFTYASIIFTAILGFILFGESPDFYATLGYVVIIGASYYMF
                     EKARRDAKIIKK"
     gene            5874..6374
                     /gene="queF"
                     /locus_tag="POB55_002078"
     CDS             5874..6374
                     /gene="queF"
                     /locus_tag="POB55_002078"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HDI6501033.1"
                     /translation="MAHGRQQDELQDITLLGNQDNTYNFDYRPDVLESFDNKHQGRDY
                     FVKFNCPEFTSLCPITGQPDFATIYISYIPNVKMVESKSLKLYLFSFRNHGDFHEDCM
                     NIIMNDLIELMDPHYIEVWGKFTPRGGISIDPYTNYGRPNSKYEKMAEHRLMNHDLYP
                     EKIDNR"
BASE COUNT         2044 a         1113 c          961 g         2338 t
ORIGIN      
        1 accataattt caatagcctt tcaaataatt tagtgtcaat tttattgata tttgcaatta
       61 aactgaaatt taatttttcg gaatgtgtat tttgtttgct taaagtaaaa tagaacactt
      121 aagattttga tgtctgggaa tagtggaaat gataaaaact actaatgatt gattatgtag
      181 tggttcttaa cattagcctc agctaatgtg tacttaaaaa taggaataca tgagtaaaac
      241 tcatgtggaa gtgataattt ctaaaggaaa aagtttttct ttatgttggg gccccgcccg
      301 aacttgcaca atattgttag ctgactttcc gcctgctttt atgttggggc cccgccaact
      361 tgcacattat tgttagctga ctttccgtct gcttctatgt tggggccccg ccaacttgca
      421 caatattgtt agctgacttt ccgcctgctt ctgtgttggg gccccgccaa ctactgccaa
      481 tataaataac attgtacatc ttagagcatt gatttcttac tcagactcat aatgagttag
      541 atacacataa aaaaagacct tcaacatgta ggagacaaga cgaaacgaat cgctatttcc
      601 ctttacatgt gaaggtcttt taatttaatt gttatttgtt gtcaaataca aagtcatcat
      661 cttgtaatgc ttctacgttt aatgctaata cataaccatc accttttact gagaagaagt
      721 catggttttt agttgttgta tctaaggcat tctcaatgat tgggttaaat tcacgttcct
      781 caaaataagg ttcaaagcct aagtttgaaa gtgctttgtt tccattatat ttaacatagt
      841 ttagcacatc ttcagtgatt ccaagatcat cgtataacat ttttgtgtat gactcttcat
      901 ttaaatacaa gtcatttagc aatttataca tttcttgatc tgctttttgt ttctcacttt
      961 cagatagttc atttcgtaaa tgctgtgcat ctaaaccggt aaatacacca tgaatagatt
     1021 catctaaaag aattttacga atgatttcac ctgatgtcgt cattttccct tgaccagcaa
     1081 gatatagtgg ataatagaaa cctgagaaga ataagaatgt ttctaaaaat acactcgtaa
     1141 ctctggccat atattggtcg tatatcgaag cttctttacc ccaaagtttg tgataattag
     1201 caacaatttt atcagattta tattttaaat ggggttcctc taaaacccat tcatctaata
     1261 ggtagtttgt ttcactagat ggtaatagtg ttgtgaaaat atgtgaatag cttttcgcgt
     1321 gtatttgctc catcatcgcc ataaatgaat aaactgcttt tttccttaag tcagtcgtat
     1381 gtagcataac taaaggcatg ccatcatctg cttgatgtgt atctaagcct gttaaaccag
     1441 ctaatgcttt tttaaatgtg tcttgttcag cttcagataa agtcttccaa cttgcaatgt
     1501 cttttgatac tttaaattct gtttcaaccc acatttgaga tatattttgt ctccaaaaca
     1561 tattcgtcat atcttcttgt gtgttccaat taacagctat catgaatata atttcctcct
     1621 gtaatgttac tcaataattg acaaatacag acatagcagt ctaatcatct gctaattcag
     1681 accagatgat tagctgtttg tcattttcaa catttaattg ttagatagaa caacttgtac
     1741 attcttctac acttaataat ttatttctag tatagtaaag tgattttaat cctttatagt
     1801 gcgcatatac atataatctt gctaactcac gtgtagaaat ttcagaatta acataaagga
     1861 tcgttgagat accttggtca atatgcgttt gaattgtcgc aattaaatcg attaatttca
     1921 tctgatcagt attgaatgct gatttgtagt accacattgt ttgtggtgat aagaatggca
     1981 tagggtaaaa tgtttccgca ttaccataag tacgacgttc aatttggtca acgattggca
     2041 ttacagaact tgttgcattt tgaacataag aaatactttg tgttggagca attgctaatc
     2101 tatatgcatg atataaacca tattgttcaa catcttgttg tagtttcttc caatcctcag
     2161 aagtaggaat agccatacca tcgaataatt cacgtacttt ttcgaattga ggttcaaatt
     2221 cttgagttgt atagaactcg aaatatttgc cattagcata atcagacttt tcaaagtctt
     2281 gatatttgat accacgctct ttagcgattt ccattgaacg ttcgattgag tagaaattca
     2341 tcatcataaa gaagatattt gcaaaatctt ttgcttcttc tgactcataa ccaattttat
     2401 tttttgctag gtaaccgtgt aaattcatga caccaagacc aactgaatgt aattcactgt
     2461 tagcttttct aactcctggt gcattttgaa tatttgctac atcactcaca acagttaatg
     2521 cgtccatacc agagtgaact gaatctctga attttccgct ttccattaca ttaacaatat
     2581 ttaatgagcc caagttacaa gaaatatcac gtttaatttc gtcttcaata ccatagtcat
     2641 taataattga agtttcttgt agttggaaaa tttccgtaca taagttactc attttaattt
     2701 gtccaatgtt tgaattcgga tgcactctgt tagcattatc tttaaacatt aaatatggat
     2761 aacctgattg taattgtgtt tgcgcaatta aattcaacat ttcacgcgca ttctttttct
     2821 ttttctcaac atttgggttt gcaaccatgt catcataata tttttctaaa tcgatatcgt
     2881 ctaatgtcac accatattct tctttaactg tatgaggtgc aaacatataa aagtccttac
     2941 cttctttagc taaatcgaag aatttagatg gaacaattaa accagttgat attgtagata
     3001 aacgtaaatc ttcatccgca tttacttttt tagtatctaa aaattcttct acatcataat
     3061 ggaagatatt taagtacaca gcaccagcac cagggcgttg accaagttga tctgcatagc
     3121 taaagccacc ttcaagtgac ttagcaatag gtaaaacgcc tttcgctacg cctttaattc
     3181 ctttaattgc ttcaccacgt gcacgcaatt tagataagtt aattgcaacg ccgcccccaa
     3241 ttttacttaa ttgttttgca gttgaatcaa taaagttaat tgaatttaag ctgtcatcca
     3301 cttctaataa gaaacatgac actagctcac cacgacgcgc acggcctgcg tttaaaaatg
     3361 ttggtgtcgc tggttgatat ctttgttcaa ccatagcaga aataaattgt ttagcttgtg
     3421 ctttattacc atttgctagg tataaagcaa caatggcaac gtgttgatta tagtcttcta
     3481 agtattgact tttatcattt gttttcaaag cgtaatcttt gaaaaattta ctagctgaca
     3541 tataacttgc aaaattaaac gggattgatt ttgcataatc agtgatttca attagatccg
     3601 cttcactata aatatcaaac acattgaaat aaaaatcgtt gtctactaaa taacgtaaac
     3661 gctcgatttc agtgtcgaag aagattgttt tgtcttttac ttcttctaaa taagctacta
     3721 aagcttcttg gtctttttct aaactaaaga atccatcttc tcttcgttta gtgacctcat
     3781 tatttaattc aatatgattg tactttttct cttccatggt tttcattaaa attacccacc
     3841 ttgttcttaa attcaataac gtctttgttt tttccatgta actcaaactt cattaataaa
     3901 gggacattat actcttctga tatcgtgcga cccgcttttg cgaaatttag tccccaattt
     3961 cgattaccgc tagctgccac acctctgatg tattgatgat taacttctaa aaaagattga
     4021 acgggttctg gtacttctcc aaatccaata gtgccagtaa cgataataaa cggttcatga
     4081 actggttcca tacaattttc tgctgtaatc tcaagcgtat tttcaagttc tgttctctta
     4141 ataaaacgac ggacatttcc agtaaatgaa aaatatatta ttttcattgg atcaccttct
     4201 ttcttcaacc cttttaaata aagtagtcat tccaattaaa aaatgcttta acaacatggt
     4261 tactatggtt atctcagcat tgtattaact ataatgaagc tacttatttt ctcaaatgta
     4321 aaccaattgc atagatttat atttgagaaa atagtagctt atatgggttg aatgtataaa
     4381 aatcactata tgtagtattt acatcattgg atatcttcca tttgctcagt acattttcta
     4441 aaaatatgac tattaattga taataactat taaatttctg tgttaaatta cttttaaatg
     4501 taatttcttc ataaataaag catttgcata caaaacacaa gatattgtgt ctttttcgtt
     4561 tcaccaacac tatattatgt gtttcattat acataagaca cactatgatt taaagtgcca
     4621 ttttctaatc gaatgttcgt aaaacgttcc aactaggcac caatactaaa ttagaaaaac
     4681 ttgaacaaat ttaaaaaaat tttttagtag atatcacttg atttttttaa atctgtgtca
     4741 atctcaattg tatcaacaga aatcaagaaa aagcttatta gcttgatact gaattgatat
     4801 ttgttttatg taataacaca aacattcata aaagtgtaat gccctattat tccgacaaat
     4861 aaacacagaa catttgttcg gtacagtcta ttatagtctt tttatagtaa atttcaacta
     4921 tttatgcaca acatgttgtt ttatgctaat ataaatacgt taattttata taaaagaggc
     4981 gataaaattg aatcctaaag taaaaggtat cattgccatt ttaatttcgg ctattggttt
     5041 cagttttatg tcagtgtttt tcagacttgc aggtgacctt cctgtttttc aaaaatcatt
     5101 agcacgaaac ttagtagcaa tgtttattcc attatttttt atatataaat accatcagcc
     5161 aatgtttggc agactttcaa gtcaaccctt attaataaca cgatcaacac ttggacttat
     5221 tggtgtattg ttaaatattt atgccattga tcatatggta cttagtgatg ccgattcatt
     5281 aatgaaactc aatcctttct ggacgatttt attaagcatt gttttcttac atgaaaaggt
     5341 aaggaagtat caaattacag caatgataat tgctatttta ggtatgcttt taatagtgaa
     5401 acctgaattt tcatcatcta tgattccatc attagctggc ttattctcag gtatttttgc
     5461 agcatcagca tatacatgtg ttcgagcact tagtacaaga gaagcaccat atactatagt
     5521 attttacttc tccttatttt ctgtcatcgt tttaatacct ttcactgcat atacctatga
     5581 accaatgagt cagatgcaaa tactttatct attaggggct ggcttagctg ccgctgtggg
     5641 tcaaattggt gttacattag catatagttt tgcagcagca aaagatattt ctattttcac
     5701 gtatgcatct ataattttca cagcaatact tggatttatt ctattcggag aatctccaga
     5761 tttctatgca acattaggtt acgtcgttat tatcggtgca agttattaca tgtttgaaaa
     5821 agcacgccga gacgccaaaa taataaaaaa ataaatttaa aggagcgctt tatatggcac
     5881 atggccgtca acaagatgaa ttacaagata taactttatt aggaaatcaa gacaatactt
     5941 acaattttga ctatcgtcct gatgtattag aatcgtttga taataagcat caaggtcgtg
     6001 actatttcgt aaaattcaac tgtccagaat ttacatcatt atgtccaatt actgggcaac
     6061 cagattttgc aactatctat atttcatata ttccaaatgt taaaatggtt gaatcaaaat
     6121 ctttgaaatt atacttattt agtttcagaa atcacggtga ttttcacgaa gattgtatga
     6181 atattattat gaatgatttg atagagctta tggacccaca ttatattgaa gtctggggca
     6241 agttcacacc acgtggtgga atttctattg atccttatac aaactatgga cgtccaaatt
     6301 ctaaatatga aaaaatggct gagcatcgtt tgatgaatca tgatttatat cccgaaaaaa
     6361 tagataatcg ttaaatgtat catttaataa acacaccaat aagttgattt tcctaactta
     6421 ttggtgtgtt tttcatttag catacataat aggtta
//
LOCUS       DAMZKC010000137         6427 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.139, whole genome shotgun sequence.
ACCESSION   DAMZKC010000137 DAMZKC010000000
VERSION     DAMZKC010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6427
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.139"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(15..1991)
                     /gene="sfaD"
                     /locus_tag="POB55_002079"
                     /gene_synonym="sfnaD"
     CDS             complement(15..1991)
                     /gene="sfaD"
                     /locus_tag="POB55_002079"
                     /gene_synonym="sfnaD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ornithine--citrate ligase SfaD"
                     /protein_id="HDI6501034.1"
                     /translation="MNLNLIFKEQTLKFNKEEQETYLFLQQHNSDWANIFKEMILQGR
                     DKVTQRLVTSMHRENLVKARTQSKKILSRDLIMLDISTTHILEIQFPQAKQTLYAPIT
                     GEHAFDRIDVEGPFYIKDDITNTITRVHHPNEILECILIEAPDLKNAASDQFQQDLIN
                     SATNMTFAISYQALSMQHDSAPLFNIIENSEDSYLRSEQAVIEGHPLHPGAKLRKGLN
                     ALQTFLYSSEFNQPIKLKIVLIHSKLGRTMSLSKDYDTTVHQLFPELIKQLENEFTPK
                     FNFNDYHIMIVHPWQLDDVLHSDYQAEVDKELIIEAKHTLDYYAGLSFRTLVPKYPAM
                     SPHIKLSTNVHITGEIRTLSEQTTHNGPLMTRILNDILEKDVIFKSYASTIIDEVTGI
                     HFYNEQDEVDYQTERSEQLGTLFRKNIYQMIPQEVTPMIPSSLVATYPFNNESPIVTL
                     IKRYQSAASLSNFESSAKSWIETYSKALLGLVIPLVTKYGIALEAHLQNAIATFRKDG
                     LLDTMYIRDFEGLRIDKVQLNEMGYSTSHFHEKSRILTDSKTSVFNKAFYSTVQNHLG
                     ELILTISKASNDSNLERHMWYIVRDVLDNIFDQLVLSTHKSNQVNENRINEIKDTMFA
                     PFIDYKCVTTMRLEDEAHHYTYIKVNNPLYRENN"
     gene            2093..3286
                     /gene="sfaA"
                     /locus_tag="POB55_002080"
     CDS             2093..3286
                     /gene="sfaA"
                     /locus_tag="POB55_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin A export MFS transporter"
                     /protein_id="HDI6501035.1"
                     /translation="MTKYFFSSSFLLFLGNWIGQIGLNWFVLTTYHNAVYLGIVNFCR
                     LVPILLLSVWAGAIADKYDKGRLLRITISSSFLVTAILCVLTYSFTAIPISVIIIYAT
                     LRGILSAVETPLRQAILPDLSDKISTTQAVSFHSFIINICRSIGPAIAGVILAVYHAP
                     TTFLAQAICYFIAVLLCLPLHFKVTKIPEDATRYMPLKVIIDYFKLHMEGRQIFITSL
                     LIMATGFSYTTLLPVLTNKVFPGKSEIFGIAMTMCAIGGIIATLVLPRVLKYIGMVNM
                     YYLSSLLFGIALLGVVFHNIVIMFICITLIGLFSQWARTTNRVYFQNNVKDYERGKVL
                     SIIMMDRGMIPLGSLLMSICADVFGIVRTFSIMGISTICITMVFYFINRKLKLKLEES
                     NHGIS"
     gene            3273..5030
                     /gene="sfaB"
                     /locus_tag="POB55_002081"
     CDS             3273..5030
                     /gene="sfaB"
                     /locus_tag="POB55_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin A synthetase SfaB"
                     /protein_id="HDI6501036.1"
                     /translation="MVYLEWAKADRNIQYRVINAIIKERIYPEQTFISQKGSLIEIQY
                     HMHVLTIEIVRKSALERYEFTGDITYLNKGETSLIITLEGLLDVLNHDFDIPISERLR
                     EELIHSRDSLVETYKQMSHRQTLISQSFKFSRLPQDINFFSWLQHVKDSDKTDDLTYS
                     ESLVPEGHPTHPLTKTKLPLTMEEVRAYAPEFEKEIPLQIMMIEKDHVVCTAMDGNDQ
                     FIIDEIIPEYYNQIRVFLKSLGLKSEDYRAILVHPWQYDHTIGKYFEAWIAKKILIPT
                     PFTILSKATLSFRTMSLIDKPYHVKLPVDAQATSAVRTVSTVTTVDGPKLSYALQNML
                     NQYPGFKVAMEPFGEYANVDKDRARQLACIIRQKPEIDGKGATVVSASLVNKNPIDQK
                     VIVDSYLEWLNQGITKESITTFIERYSQALIPPLIAFIQNYGIALEAHMQNTVVNLGP
                     HFDIQFLVRDLGGSRIDLETLQHRVSDIKITNDSLIADSIDAVIAKFQHAVIQNQMAE
                     LIHHFNQYDCVEEAELFNIVQQVVAHAINPTLPHANELKDILFGPTITVKALLNMRME
                     NKVKQYLNIELDNPIKKEV"
     gene            5037..6107
                     /gene="sfaC"
                     /locus_tag="POB55_002082"
     CDS             5037..6107
                     /gene="sfaC"
                     /locus_tag="POB55_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin A biosynthesis protein SfaC"
                     /protein_id="HDI6501037.1"
                     /translation="MAHVNINISKIKYNAKVLQTVFQSKNMQFTPVIKCIAGDRTIVE
                     SLKALGINHVAESRLDNIISIADQDLTYTLLRTPAKKEISDMIEKVDMSIQTELSTIH
                     QINEVAEVLGKKHKILLMVDWKDGREGVLTYDVLDYIKEIIHLKNIHFVGLAFNFMCF
                     KSDAPSDDDIFMINRFVSAVEREIGYRLKIISGGNSSMLPQLLYNDLGKINELRIGET
                     LFRGVDTTTNQAIAMLYQDAITLEAEILEIKPRVNTQTHESFLQAIVDIGYLDTKVDN
                     ISPMDQHINILGASSDHLMLDLNGQGHYQVGDHISFSLNYEALSHSMYMKNLHKVYID
                     DSKIDTLLQNFDVKSPAMVNQY"
     gene            complement(6208..6333)
                     /locus_tag="POB55_002083"
     CDS             complement(6208..6333)
                     /locus_tag="POB55_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501038.1"
                     /translation="MYHCYIIFALIQLTPFSMTVIPVIQLILSVAILCDELLHLI"
BASE COUNT         2175 a          912 c         1127 g         2213 t
ORIGIN      
        1 aaaattctat aaccttaatt attttctcga tacaaagggt tattaacttt aatataagta
       61 taatgatgtg cctcatcttc aagacgcatt gttgtaacac acttataatc tataaatggc
      121 gcgaacatgg tatctttaat ttcatttatg cgattctcat ttacttgatt agatttgtgt
      181 gtcgaaagta caagttgatc aaaaatgtta tctagtacat cacgaacgat ataccacata
      241 tgtctttcta agtttgaatc atttgatgct ttagagattg taagaattaa ttcgcctaaa
      301 tggttttgaa cggtagaata aaaggctttg ttaaacacag acgtttttga atcagtaaga
      361 attcttgatt tttcatggaa atgagatgta ctgtatccca tttcatttag ttgtacttta
      421 tcaattctta aaccttcaaa gtctctaata tacatcgtgt ccaatagacc atctttccga
      481 aatgttgcaa tagcattttg caagtgagcc tctaatgcaa tgccatattt agtaactagt
      541 gggattacga gaccaagcaa tgctttacta taagtttcaa tccacgattt cgccgatgat
      601 tcaaaattag acaatgatgc agctgattga taacgtttaa tcaatgtcac gataggtgat
      661 tcattgttaa atgggtaggt tgcaactaag cttgaaggaa tcattggtgt gacttcttgt
      721 ggaatcattt ggtatatgtt ttttctaaat aatgtaccta attgttcact tcgttcagtt
      781 tgatagtcta cttcgtcttg ctcattataa aaatgaatac cagtaacttc atcaataatt
      841 gttgatgcat aggacttaaa tatgacatct ttctccaaaa tatcatttaa aatacgtgtc
      901 attagtggac cattgtgcgt cgtttgttct gataatgtac gaatttcacc tgtaatatga
      961 acgtttgtcg acaatttgat gtgtggcgac atagctgggt atttaggaac taatgttctg
     1021 aaagataaac cggcataata atccaacgta tgttttgctt caatgattaa ttctttatct
     1081 acttctgctt gataatcaga atgtaatacg tcatctaatt gccatggatg aacaatcata
     1141 atgtgataat cattaaagtt aaactttggc gtaaattcat tttctaattg tttaattaat
     1201 tccggaaata gttgatgaac agttgtatca taatctttag acagtgacat agtccggcct
     1261 aatttactgt gaatcagtac tattttcaac ttaataggtt gattgaattc tgaagaatat
     1321 aggaatgttt gtaatgcatt taaaccttta cgtaatttag ccccaggatg tagcggatga
     1381 ccttcaataa cggcttgctc tgaacgcaag taactatctt cgctattttc gataatatta
     1441 aataaaggtg cagaatcatg ttgcattgac agtgcttgat agctaattgc aaatgtcata
     1501 ttagttgcac tgtttattaa atcttgctga aattgatcac tagcagcatt ttttaaatct
     1561 ggtgcttcaa ttaaaataca ctcaagaatt tcatttggat ggtgtactcg tgtaatcgta
     1621 tttgtaatgt catctttaat gtagaaaggg ccttcaacat caattcgatc aaaggcgtgt
     1681 tctccagtga taggagcata taatgtttgc ttagcttgtg ggaactggat ttctagtata
     1741 tgagtcgttg agatatctaa cataatcaaa tcacgactca atattttctt actttgagtg
     1801 cgcgctttaa ctaagttttc gcgatgcatt gatgtgacta atctctgagt gactttatct
     1861 cttccttgta aaatcatctc tttaaaaata ttagcccaat cgctattatg ttgttgtaaa
     1921 aataaatatg tttcttgttc ttctttgtta aattttaatg tctgttcttt aaaaattaag
     1981 ttcaagttca taattcacct ctatgaaata ttttacaaaa gcaagataga tttgtataat
     2041 ccatattaat gataatgatt cttattatca acagaatgcg ggtgtaagtt ttatgacaaa
     2101 atattttttt agcagttctt ttctactatt tctaggtaat tggattggac aaatagggct
     2161 aaattggttt gtacttacca cttatcataa cgcagtttat ctggggattg tcaatttttg
     2221 cagacttgta ccaatattat tactaagtgt gtgggcaggg gcaattgccg ataaatatga
     2281 taaagggcga ttgctgagaa ttacaatttc atcatcattt ttagtaactg caattttatg
     2341 tgtgctcacg tatagtttca ctgcaattcc aattagcgtc attattatat atgcgacatt
     2401 aagaggaatt ttaagtgctg ttgaaacacc tttaagacaa gcaatcttac cagatttatc
     2461 agataaaata tctactacac aagctgtatc atttcattca ttcatcatta atatttgtcg
     2521 ttcaataggg cctgccattg ctggtgtcat attagcagtc tatcatgcgc caacaacatt
     2581 tcttgcacag gcaatttgtt attttatcgc agttttatta tgtttaccat tacattttaa
     2641 agtaactaaa atacctgaag atgcaacaag atacatgccg ttaaaagtta ttatagatta
     2701 cttcaaatta catatggaag gtcgacaaat atttataaca tcattattga ttatggcgac
     2761 aggtttttca tatacgacac ttttaccagt tttgacaaac aaagtatttc cggggaaatc
     2821 tgaaatattt ggtatcgcta tgacgatgtg tgccattggt ggtattattg caacgctagt
     2881 tttacctaga gtacttaaat atattggtat ggtaaatatg tattatttaa gttcactttt
     2941 atttggcatt gctttgttag gtgtggtatt tcacaatatt gtcatcatgt tcatttgtat
     3001 tacattgatt gggttattta gtcaatgggc acgtacgaca aatcgcgttt attttcaaaa
     3061 taatgttaaa gattatgaac gtggtaaagt actgagtatt attatgatgg atagaggtat
     3121 gattccattg ggaagtctgt taatgagtat atgtgcagat gtgtttggca ttgttagaac
     3181 tttttcaata atgggaataa gtactatatg cattacaatg gtattctatt ttataaatag
     3241 aaagttgaag ttaaagttgg aggaaagtaa tcatggtata tcttgaatgg gcaaaggcag
     3301 atagaaatat tcaatatcgt gtaattaacg ccattattaa agaacgtatt taccccgagc
     3361 aaacatttat ttcgcaaaaa ggatctttaa ttgaaattca gtatcatatg catgtgttga
     3421 ctattgaaat tgttagaaaa agtgcattag aacgctacga gtttacaggt gatattactt
     3481 atttaaataa aggtgaaacg tcattaatta taactttaga aggtttatta gatgtgttga
     3541 atcatgactt tgatatccct atttcagagc gactacgcga agagttaata cacagtcgag
     3601 atagtttagt tgaaacatat aagcaaatgt ctcacagaca aacgttaata agtcaaagtt
     3661 ttaaattttc aagattacca caagatatta actttttttc atggttacaa catgtaaaag
     3721 atagtgataa gacagatgat ttaacttatt ctgagagttt ggtaccagag gggcatccaa
     3781 cacacccttt aaccaaaacg aaattgccct taactatgga agaagtacga gcatatgcac
     3841 ctgagtttga aaaagaaatc cctttgcaaa ttatgatgat tgaaaaagac catgttgtgt
     3901 gcacagctat ggatggtaat gatcaattta ttattgatga aataattcct gaatactaca
     3961 atcagattcg tgtgtttttg aagagtttag gtttgaaaag tgaagactat agagcgattt
     4021 tagtacatcc ttggcaatat gatcatacga tagggaaata ttttgaagca tggattgcta
     4081 aaaaaatatt aattccaacg ccgtttacaa tactttcaaa agcaacttta tcattcagga
     4141 cgatgtcttt aattgataaa ccatatcatg ttaagttgcc cgtcgatgca caagcaacaa
     4201 gtgccgttag aacagtctca actgtgacta ctgtagatgg accaaagtta agttatgctt
     4261 tacaaaacat gttgaatcaa tatccaggat ttaaagttgc tatggaaccg ttcggtgaat
     4321 atgcaaatgt tgataaagat agggcacgtc agcttgcttg tatcataaga caaaagcctg
     4381 aaattgatgg aaaaggtgca acagtagtta gtgcaagtct agttaataaa aatccaatag
     4441 atcaaaaagt tatcgtggat agttacttag agtggttaaa tcaaggaatt actaaagaaa
     4501 gtattacgac atttattgaa cgatactctc aagcattaat cccgccttta attgctttta
     4561 ttcaaaatta tggaattgca ttagaagcac acatgcaaaa tacagtagtg aacttggggc
     4621 cacattttga cattcaattt ttagtgagag atttaggtgg ttctagaatt gatttagaaa
     4681 cattacaaca tcgtgtatca gatattaaaa ttacaaatga tagtttaata gctgattcta
     4741 tagatgcagt gattgcaaaa ttccaacatg ctgttattca aaatcaaatg gcagaattaa
     4801 tccatcattt taatcagtat gattgtgttg aagaagccga attatttaac atagtacagc
     4861 aagtagtagc gcatgccatt aacccaacac taccacatgc aaatgagtta aaagatattt
     4921 tgtttggacc aacaattact gtcaaagcgt tgttaaatat gagaatggaa aataaagtaa
     4981 agcaatattt aaatattgag ttagataatc cgataaaaaa agaggtgtag tactacatgg
     5041 cacacgttaa cataaatata tcgaagatta aatataacgc caaagtactt caaacagttt
     5101 ttcaaagtaa aaatatgcaa ttcacaccag taattaagtg catagctggt gaccgtacaa
     5161 ttgtagaaag cttaaaagcg ttaggtatca atcatgttgc agaatccaga ttggataaca
     5221 taattagtat tgcagatcag gatttaacat atacgttatt gcgtacgcct gccaaaaaag
     5281 agatttcaga tatgatagaa aaagttgata tgagtatcca aacagaatta tctaccatac
     5341 atcaaataaa tgaagtagca gaagttttag gaaaaaaaca taagatttta ttaatggtag
     5401 attggaaaga tggccgtgaa ggtgttctga cttatgatgt tttagattat attaaagaga
     5461 ttattcactt gaaaaatatc cattttgtag gtttagcatt caattttatg tgttttaaat
     5521 ctgatgcacc aagtgatgat gatatcttta tgattaatcg attcgtctca gctgttgaaa
     5581 gagaaatagg atatcgattg aaaattattt caggtggtaa ttcgagcatg ttaccacaac
     5641 ttttatataa tgatttgggt aaaataaatg aattgcgaat aggtgaaacg ttgtttagag
     5701 gtgttgatac aacgacaaat caagcgatag ctatgttata tcaagatgcg attacattgg
     5761 aggctgagat tttagaaatt aaacctcgag ttaatacaca aacgcatgaa tcattcttac
     5821 aagctattgt agatattggt tatttagata caaaagtcga taatatttca cctatggatc
     5881 agcatatcaa tattttaggc gcttcgagtg atcatttaat gcttgattta aatggtcaag
     5941 gacactatca agttggagat catatatctt tttcattaaa ttatgaagca ctttctcatt
     6001 cgatgtatat gaaaaattta cataaggtat atattgatga ttcaaagatt gatacgctac
     6061 ttcaaaattt tgatgtgaaa tctcctgcta tggtaaacca atattaatat atttatcaat
     6121 aagtctaagt tgacaagtta tactacatat cgtttgcacg gttgtatcat aattgttcaa
     6181 cttagatttt ttgtatttgt tgatttatca aattaagtgc aacagttcgt cacataaaat
     6241 tgcaacagat aatatcagct gaattacagg gataacggtc atactaaatg gtgtcaattg
     6301 tattaatgca aagatgatat agcaatgata cattatcaat attttttcta aggaaatgtg
     6361 ctaattgtag tcataattac tagaggaaat atatagtcat acattttagt aatataaaaa
     6421 agattga
//
LOCUS       DAMZKC010000138         6287 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.140, whole genome shotgun sequence.
ACCESSION   DAMZKC010000138 DAMZKC010000000
VERSION     DAMZKC010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6287
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.140"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            96..812
                     /locus_tag="POB55_002084"
     CDS             96..812
                     /locus_tag="POB55_002084"
                     /EC_number="2.7.7.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribitol-5-phosphate cytidylyltransferase"
                     /protein_id="HDI6501039.1"
                     /translation="MKYAGILAGGIGSRMGNVPLPKQFLDLDNKPILIHTLEKFILIN
                     DFEKIIIATPQQWMTHTKDTLRKFKISDERIEVIQGGSDRNDTIMNIVKHIESTNGIN
                     DDDVIVTHDAVRPFLTHRIIKENIQAALEYGAVDTVIDAIDTIVTSKDNQTIDAIPVR
                     NEMYQGQTPQSFNINLLKESYAQLSDEQKSILSDACKIIVETNKPVRLVKGELYNIKV
                     TTPYDLKVANAIIRGGIADD"
     gene            805..1830
                     /locus_tag="POB55_002085"
     CDS             805..1830
                     /locus_tag="POB55_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HDI6501040.1"
                     /translation="MINQVYQLVAPRQFEVTYNNVDIYSDYVIVRPLYMSICAADQRY
                     YTGSRDENVLSQKLPMSLIHEGVGEVVFDSKGVFNKGTKVVMVPNTPTEKDDVIAENY
                     LKSSYFRSSGHDGFMQDFVLLNHDRAVPLPDDIDLSIISYTELVTVSLHAIRRFEKKS
                     ISNKNTFGIWGDGNLGYITAILLRKLYPESKIYVFGKTDYKLSHFSFVDDVFFINKIP
                     EGLTFDHAFECVGGRGSQSAINQMIDYISPEGSIALLGVSEFPVEVNTRLVLEKGLTL
                     IGSSRSGSKDFQDVVDLYIQYPDIVDKLALLKGQEFEIATINDLTEAFEADLSTSWGK
                     TVLKWIM"
     gene            1852..3540
                     /locus_tag="POB55_002086"
     CDS             1852..3540
                     /locus_tag="POB55_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HDI6501041.1"
                     /translation="MVKSKIYIDKIYWERVQLFVEGHSENLDLEDSNFVLRNLTETRT
                     MKANDVKIDGNQFVCRFNVAILDNGYYLPEDKYLLVNEQELDYIAQLNPDVINDAYQN
                     LKPEQEEEYNELETQNGKINFLLQTYLKEFRKGGISKKTVYTVTPEISSDVNEFVLDV
                     VVTTPEVKSIYIVRKYKELRKYFRKQSFNTRQFIFKAIFNTTKFFHLKKGNTVLFTSD
                     SRPTMSGNFEYIYNEMLRQNLDKKYDIHTVFKANITDRRGIIDKFRLPYLLGKADYIF
                     VDDFHPLIYTVRFRRSQEVIQVWHAVGAFKTVGFSRTGKKGGPFIDSLNHRSYTKAYV
                     SSETDIPFYAEAFGIKEKNVVPTGVPRTDVLFDEAYATQIKQEMEDELPIIKGKKVIL
                     FAPTFRGSGHGTAHYPFFKIDFERLARYCEKNNAVVLFKMHPFVKNRLNIADKHKQYF
                     VDVSDFREVNDILFITDLLISDYSSLIYEYAVFKKPMIFYAFDLEDYITTRDFYEPYE
                     SFVPGKIVQSFDALMDALDNEDYEGEKVIPFLDKHFKYQDGRSSERLVRNLFGS"
     gene            3573..5294
                     /gene="tarS"
                     /locus_tag="POB55_002087"
     CDS             3573..5294
                     /gene="tarS"
                     /locus_tag="POB55_002087"
                     /EC_number="2.4.1.355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly(ribitol-phosphate)
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDI6501042.1"
                     /translation="MMKFSVIVPTYNSEKYITELLNSLAKQDFPKTEFEVVVVDDCST
                     DQTLQIVEKYRNKLNLKVSQLETNSGGPGKPRNVALKQAEGEFVLFVDSDDYINKETL
                     KDAAAFIDEHHSDVLLIKMKGVNGRGVPQSMFKETAPEVTLLNSRIIYTLSPTKIYRT
                     TLLKDNDIYFPEELKSAEDQLFTMKAYLNANRISVLSDKAYYYATKREGEHMSSAYVS
                     PEDFYEVMRLIAVEILNADLEEAHKDQILAEFLNRHFSFSRTNGFSLKVKLEDQPQWI
                     NALGDFIQAVPERVDALVMSKLRPLLHYARAKDIDNYRTVEESYRQGQYYRFDIVDGK
                     LNIQFNEGEPYFEGIDIAKPKVKMTAFKFDNHKIVTELTLNEFMIGEGHYDVRLKLHS
                     RNKKHTMYVPLSVNANKQYRFNIMLEDIKAYLPKEKIWDVFLEVQIGTEVFEVRVGNQ
                     RNKYAYTAETSALIHLNNDFYRLTPYFTKDFNNISLYFTAITLTDSISLKLKGKNKII
                     LTGLDRGYVFEEGMASVVLKDDMIMGMLSQTSENEVEILLSKDIKKRDFKNIVKLNTA
                     HMTYSLK"
     gene            5438..6112
                     /gene="scdA"
                     /locus_tag="POB55_002088"
     CDS             5438..6112
                     /gene="scdA"
                     /locus_tag="POB55_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein ScdA"
                     /protein_id="HDI6501043.1"
                     /translation="MINKNDIVADVVTDYPKAADIFRSVGIDFCCGGQVSIEAASLEK
                     KNVDLNELLQRLNDVEQTNTPGSLNPKFLNVSSLIQYIQAAYHEPLREEFKNLTPYVT
                     KLSKVHGPNHPYLVELKETYDTFKNGMLEHMQKEDDVDFPKLIKYEQGEVVDDINTVI
                     DDLVSDHIATGQLLVKMSDLTSSYEPPIEACGTWRLVYQRLKALEVLTHEHVHLENHV
                     LFKKVS"
BASE COUNT         2302 a          846 c         1160 g         1979 t
ORIGIN      
        1 tttagagttt tgattttaaa atgagttttc aattgaaaaa tgcaacgaaa tttaaataat
       61 taaatataga tatagttgaa tggaggaagt attttatgaa atacgctggt attctagctg
      121 gaggtatagg ctcaagaatg ggtaacgtac ctttacctaa acaattttta gatttagaca
      181 acaaaccgat tttaatccat acattagaaa aatttatttt aattaatgat tttgaaaaaa
      241 ttattatcgc gacgccacaa caatggatga cgcatacgaa agatacactt agaaaattca
      301 aaatttctga tgaaagaatt gaagtcattc aaggtggtag cgatcgtaac gatacaatta
      361 tgaatatcgt taaacatatt gaatcaacaa atggtattaa cgatgacgat gtcattgtga
      421 cacatgatgc agttagacca tttttaacgc atcgtattat taaagaaaat attcaagctg
      481 ctttagagta cggtgcagta gatacagtga ttgatgctat agatacgatt gttacatcta
      541 aagataatca aacgattgat gcaattccag tgcgtaatga aatgtaccaa ggtcaaacac
      601 ctcaatcgtt taatattaat ttattaaaag aaagctatgc acagttgagt gatgagcaaa
      661 agagtatttt atctgatgct tgtaagatta ttgtagaaac aaacaaaccg gttcgacttg
      721 taaaaggtga gttatataac attaaagtaa caacacctta cgatttaaaa gtagcgaatg
      781 ctattattcg aggtggtatt gccgatgatt aatcaagtat atcaattagt tgcacctaga
      841 caatttgaag ttacgtataa caacgtagat atttacagtg actatgtcat tgtacgtcct
      901 ttatatatgt caatttgtgc tgccgatcaa agatattata ctggtagccg tgatgagaat
      961 gtcttatctc agaaattgcc aatgtcttta attcatgaag gtgttggtga ggtcgtattt
     1021 gacagtaaag gtgtgtttaa taaaggtaca aaagtagtta tggtaccgaa tacgccgaca
     1081 gaaaaagacg atgtcattgc tgaaaactat ttaaaatcga gctacttcag atcaagtgga
     1141 catgatgggt ttatgcaaga ttttgtgttg ctaaatcatg atagagctgt accactacct
     1201 gatgatattg atttaagtat tatttcatat acagagcttg taacagtaag tttgcatgct
     1261 attcgtcgtt ttgaaaagaa atctatttca aataaaaata catttggtat ttggggtgat
     1321 ggtaacttag gttacattac agccatttta ttacgtaaat tatatccaga gtctaaaata
     1381 tatgtctttg gtaaaacaga ttataaattg agtcacttct catttgttga tgatgtcttc
     1441 tttattaata aaatacctga aggcttaaca tttgatcatg catttgagtg tgtgggtggt
     1501 cgcggtagtc aatcagccat aaatcaaatg atcgattaca tttcaccaga aggaagcatt
     1561 gcactgttag gtgtaagtga gttcccagta gaagttaata cacgtctagt attggaaaaa
     1621 ggactaacgt tgattggtag tagtcgaagt ggttcaaaag atttccaaga tgttgtagac
     1681 ttatacattc aatacccaga tattgtagat aaattagcgt tgttaaaagg tcaagaattt
     1741 gaaattgcaa caattaatga tcttacagaa gcttttgaag cagacctgtc tacatcttgg
     1801 ggtaaaacag tattaaaatg gattatgtaa atagaaaagg atgaattaac gttggttaaa
     1861 agtaagatat atatagataa aatctattgg gaacgtgttc agttattcgt tgaaggacat
     1921 agtgaaaacc tagatttaga agatagtaat tttgtattaa gaaatttaac tgagacacgt
     1981 acaatgaagg cgaatgatgt caaaatagat gggaatcaat tcgtttgtcg tttcaatgta
     2041 gctatcttag ataatggtta ttacttacct gaagataagt acttattagt gaatgagcaa
     2101 gaacttgatt atattgcaca gttaaaccca gatgtgatta atgatgcata tcaaaatcta
     2161 aagccagaac aagaagaaga atacaacgaa ttagaaacac aaaatggtaa aatcaatttc
     2221 ttattgcaga cttacctaaa agaatttaga aaaggcggca tttcgaagaa aacggtttat
     2281 actgttacac ctgaaatttc tagcgatgtt aatgaatttg tccttgatgt tgttgtaacg
     2341 actccggaag ttaaaagtat ttatatcgtt cgtaaatata aagaattacg taagtatttc
     2401 cgcaaacaat catttaatac aagacaattt atttttaaag cgatatttaa tacgacgaaa
     2461 tttttccact tgaaaaaagg gaatacggtg ttgtttacat cagactctag accaacgatg
     2521 tctggaaact ttgaatacat ctataacgaa atgttacgtc aaaatttaga taaaaagtat
     2581 gatattcaca ctgtttttaa agcgaatatt acagatagac gtggcatcat cgacaagttt
     2641 agattgccat atttacttgg gaaggcagac tacatctttg ttgatgactt tcacccattg
     2701 atttatacag tgcgttttag acgttctcaa gaagttattc aagtatggca tgccgttggt
     2761 gcctttaaaa cagttggctt tagtcgtact ggtaaaaagg gtggaccatt tattgattca
     2821 ttaaatcatc gtagctatac aaaagcttat gtatcatctg aaaccgatat tccattctac
     2881 gctgaagcat ttggtattaa agagaaaaat gtagtgccta caggtgttcc acgtactgat
     2941 gtactatttg atgaagctta tgcgacacag atcaaacaag agatggaaga tgaattacca
     3001 attattaaag gtaagaaagt cattcttttc gcaccaacat ttagaggtag tggtcatggt
     3061 acagcacatt acccattttt caaaattgat tttgaacgtt tagcaagata ttgcgaaaaa
     3121 aataacgcgg ttgtattatt taaaatgcat ccatttgtga aaaatagact taatattgca
     3181 gacaaacata aacaatattt tgttgacgtt tctgacttta gagaagttaa tgatatactg
     3241 ttcataacag atttattaat tagtgactat tcatctttaa tatatgaata tgcagtattt
     3301 aaaaagccaa tgattttcta tgcatttgat ttagaagatt atattacgac gcgtgatttt
     3361 tatgaaccat atgaatcatt tgttccaggt aaaattgtgc aatcatttga cgcattaatg
     3421 gacgccttgg acaatgaaga ttatgaagga gaaaaagtca ttccattctt agataaacat
     3481 tttaaatatc aagatggccg atcaagtgag cgtttagtca gaaatttatt tggtagctaa
     3541 gtttatatag tagtcaaagt gggagaggta taatgatgaa attttcagta atagttccaa
     3601 catacaattc agaaaagtat ataacagaat tacttaatag ccttgcgaaa caagattttc
     3661 cgaaaactga atttgaagtg gttgtagttg atgactgttc aacagatcaa acgttacaaa
     3721 tagttgaaaa gtatcgcaat aaattgaact tgaaagtaag tcaactcgaa acaaattctg
     3781 gtggtccagg taaacctaga aatgtggcgt taaaacaagc agaaggtgaa tttgtattat
     3841 ttgtggactc cgatgactat ataaacaaag agactttaaa agatgcagca gcatttattg
     3901 atgaacatca ctccgatgtc ttattgatta aaatgaaagg tgttaatggt cgtggtgtac
     3961 cacaatctat gtttaaagaa acagcacctg aagttacttt gttaaattca agaattatct
     4021 atactttaag cccgactaaa atctatagaa caacattact aaaagataat gacatttatt
     4081 ttccagaaga attaaagagt gcagaagatc aattatttac aatgaaagca tatttgaatg
     4141 caaatcgaat cagtgtgtta agtgataaag cgtattatta tgctacaaag cgtgaaggtg
     4201 aacatatgag tagtgcgtat gtttcacctg aagactttta cgaagtcatg agattgattg
     4261 ctgtagaaat attaaatgca gatttagaag aagctcataa agatcaaatc ttagcagaat
     4321 ttttaaatcg tcattttagt ttttctcgta cgaatggctt ctcacttaaa gttaaactag
     4381 aagatcaacc gcaatggatt aatgctctag gagactttat acaagcagtt ccagaacgtg
     4441 tagatgcatt ggtgatgagt aaattacgac cattgttgca ctacgcgaga gcgaaagata
     4501 tagacaacta tagaactgta gaagaaagtt accgtcaagg tcaatactac cgttttgata
     4561 ttgtagatgg taaattaaac attcaattca atgaaggcga accatacttt gaaggcattg
     4621 atatcgctaa gccaaaagtg aaaatgacag catttaaatt tgataatcat aaaattgtta
     4681 cagagctaac gttaaatgaa tttatgattg gcgaaggaca ttatgatgtc agacttaaat
     4741 tacattcacg aaacaagaag cacacaatgt atgtaccttt aagtgtcaat gcgaataaac
     4801 aatatcgttt taacattatg ttagaagata ttaaagcgta tttacctaaa gaaaaaattt
     4861 gggatgtttt cttagaagtc caaataggta cggaagtatt tgaagtgcgt gttggtaatc
     4921 aacgtaataa atatgcatat actgcagaaa caagtgcatt aattcatttg aataatgatt
     4981 tttatagatt aacaccgtat ttcacaaaag actttaataa catttcgtta tactttacag
     5041 ctattacatt aacggattca atctcattga agttaaaagg taaaaacaaa atcattttaa
     5101 ctggtctgga tcgtggttat gtatttgaag aaggtatggc tagtgtcgtg ctaaaagacg
     5161 acatgattat gggaatgtta agccaaacgt cagaaaacga agtggaaatc ttacttagta
     5221 aagatattaa gaagcgagac ttcaaaaata ttgttaagtt aaacactgca catatgactt
     5281 actcgctaaa ataaataata aatgccctca aatctatgtg aactaacatg atttgagggt
     5341 tttattttac tatttatgaa atgattatga catttccctg attttcattt tcatatacat
     5401 taaattgtat acactgaaaa tgaggaggtc gtctataatg ataaataaaa atgacatagt
     5461 agcagatgta gtaacagatt atccgaaagc agcagatatt tttagaagcg taggtataga
     5521 cttttgttgc ggtggacaag taagtataga agcagcatcc ttagaaaaga aaaatgtaga
     5581 tttgaacgaa ttattacagc gtctcaatga cgttgaacaa acgaatacac caggttcgct
     5641 taaccctaaa tttttaaatg tttcgtcact tattcaatat attcaagcag catatcatga
     5701 acctcttaga gaagaattta aaaatttaac accttatgtg acgaaattat cgaaagtaca
     5761 tggacctaac catccatatt tagtcgagtt aaaagaaaca tatgatacat ttaaaaatgg
     5821 catgttagag catatgcaaa aagaagatga tgttgatttt ccaaaactaa ttaaatatga
     5881 acaaggtgaa gtagtagacg atattaatac agtgattgat gatttagtat ctgatcacat
     5941 tgcaacggga caattgttag tgaaaatgag cgatttaaca tctagctatg aaccaccgat
     6001 agaggcatgt ggtacgtggc gactcgttta tcagagatta aaagcacttg aagtgttaac
     6061 acatgagcat gttcatttag agaatcatgt tttatttaaa aaagtatcat aaataacgcg
     6121 attagaaact gttggcaaag taatgtcagc agtttttcgc tatacttaac agaaatttag
     6181 tgatatgaac agcattattt gaaaagaaaa atggtcaact tagcataaaa attgatatga
     6241 aaaattaatg gtatagataa ttaaatagta gcgtgttttt taataat
//
LOCUS       DAMZKC010000139         6114 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.141, whole genome shotgun sequence.
ACCESSION   DAMZKC010000139 DAMZKC010000000
VERSION     DAMZKC010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6114
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.141"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            123..1289
                     /gene="aroC"
                     /locus_tag="POB55_002089"
     CDS             123..1289
                     /gene="aroC"
                     /locus_tag="POB55_002089"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HDI6501044.1"
                     /translation="MRYLTSGESHGPQLTVIVEGIPANLEIKVEDINKEMFKRQGGYG
                     RGRRMQIEKDTVEIVSGVRNGYTLGSPITMVVTNDDFTHWRKIMGAAPISEEERENMK
                     RTITKPRPGHADLVGGMKYNHRDLRNVLERSSARETAARVAVGALCKVLLQQLDIDIY
                     SRVVEIGGIKDKDFYDSKTFKANLDRNDVRVIDDSIAQAMRDKIDEAKNDGDSIGGVV
                     QVVVENMPVGVGSYVHYDRKLDGRIAQGVVSINAFKGVSFGEGFKAAEKPGSEIQDEI
                     LYNTELGYYRGSNHLGGLEGGMSNGMPIIVNGVMKPIPTLYKPLNSVDINTKEDFKAT
                     IERSDSCAVPAASIVCEHVVAFEIAKALLEEFQSNHIEQLKQQIIERRQLNIEF"
     gene            1315..2379
                     /locus_tag="POB55_002090"
     CDS             1315..2379
                     /locus_tag="POB55_002090"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HDI6501045.1"
                     /translation="MKLQTTYPSNNYPIYVERGAIDHISTYIDQFDQSFILIDEYVNQ
                     YFVNKFDDILSYENVHKVIIPAGEKTKTFHQYQETLEYILSHHVTRNTAIIAVGGGAT
                     GDFAGFVAATLLRGVHFIQVPTTILAHDSSVGGKVGINSKQGKNLIGAFYRPTAVIYD
                     LDFLKTLPFEQILSGYAEVYKHALLNGESTTQEIEQHFKDREILQSLNGMDKYIAKGI
                     ETKLDIVVADEKEQGVRKFLNLGHTFGHAVEYYHKIPHGHAVMVGIIYQFIVANALFD
                     SKHEINHYIQYLIQLGYPLDMITDLDFETLYEYMLSDKKNDKQGVQMVLIRQFGDIVV
                     QHVDQLTLQHACEQLKTYFK"
     gene            2389..3687
                     /gene="aroA"
                     /locus_tag="POB55_002091"
     CDS             2389..3687
                     /gene="aroA"
                     /locus_tag="POB55_002091"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDI6501046.1"
                     /translation="MVNEQIIDISGPLKGEIEVPGDKSMTHRAIMLASLAEGVSTIYK
                     PLLGEDCRRTMDIFRLLGVEIKEDDEKLVVTSPGYQSFNTPHQVLYTGNSGTTTRLLA
                     GLLSGLGIESVLSGDVSIGKRPMDRVLRPLKLMDANIEGIEDNYTPLIIKPSVIKGIN
                     YQMEVASAQVKSAILFASLFSKEPTIIKELDVSRNHTETMFKHFNIPIEAEGLSINTT
                     PEAIRYIKPADFHVPGDISSAAFFIVAALITPGSDVTIHNVGINPTRSGIIDIVEKMG
                     GNIQLFNQTTGAEPTASIRIQYTPMLQPITIEGELVPKAIDELPVIALLCTQAVGTST
                     IKDAEELKVKETNRIDTTADMLNLLGFELQPTNDGLIIHPSEFKTNATVDSLTDHRIG
                     MMLAVASLLSSEPVKIKQFDAVNVSFPGFLPKLKLLENEG"
     gene            3694..4938
                     /locus_tag="POB55_002092"
     CDS             3694..4938
                     /locus_tag="POB55_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501047.1"
                     /translation="MEDIYKLIDDINLQKLENLDSRVNEAITTDNDDALFILGETLYN
                     FGLMPQGLEVFRVLYHKYPDESELLIYFIEGLMSENQTDEALEYLSYVEPSPEKLMLE
                     ADLYQQINMMEVAIAKLQEALELEPNDPIIHFALAEMLYYDGQYLRATSEYETVLETG
                     EYQVNGVNLFSRMADCSLQSGNYSDAIRLYDEINEDEMTSEDYLKKAISYDKNDITQE
                     AIKIMTTLLSKDPDYIQGYLYLQSLYENEKNYPDAIETGKEGLRLSQFYKELMYTTGC
                     LEIEHGDANEGVLLLRQALEVDNAYQEPLLILSDLYRNEEDYEAIIELLTYVDEEDLD
                     PTFMWHLAFAYGQEERDKEAQHFFELAYPTMKTNIDFMSDYYFYLTEIGQKEQAITIL
                     NQLLELEPSNENWHDELSRLQS"
     gene            4952..5527
                     /locus_tag="POB55_002093"
     CDS             4952..5527
                     /locus_tag="POB55_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HDI6501048.1"
                     /translation="MSETLNQIKESFIEYLLFQYRFKSRIAVWVLNYIKVNEAKLANI
                     HFVDTKINHHETLEIAEVGSHASAIQFTKRNIKLMNTNEIFDYIANHNCAFDIQIHFA
                     NVSKREQRLDDLIVAQLTESPSYQTYLHDLNSMAIDRHKHALLIDYLLHNIDLSLQMN
                     EKQRFYQLTQILNTLKLVNKHNQFEDLADDD"
     gene            5517..6104
                     /locus_tag="POB55_002094"
     CDS             5517..6104
                     /locus_tag="POB55_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1405 domain-containing protein"
                     /protein_id="HDI6501049.1"
                     /translation="MTINTFWQYTLYQRSWLMMLLICNILGMIYGYIWYGEQLSHTPW
                     HFKIFVPDSPTAILFLVISISMILIQKQNSIIDALAFVTLFKYGIWAVIMNILFIIEQ
                     GDITVNGLILMFSHSIMAVQAIYFYPRFKRSMIGISVAMTWVFLNDYIDYFHLQFPYY
                     DFITTHVWQIGVLSCCLSVFGLLLYIELNKLLKCK"
BASE COUNT         2170 a          849 c         1151 g         1944 t
ORIGIN      
        1 tgagatgtca tccattttct taattaagct tgaaaacaaa catttatgaa tccacaatga
       61 atgtgataag attaacaaca tattataatg ttattgcgga agtatgaaag gagcgagtgt
      121 gtatgagata tctaacttct ggagaatcac atggacctca attaacagtt attgtcgaag
      181 gtataccagc aaatttagaa attaaagttg aagatattaa taaagaaatg ttcaagcgtc
      241 aaggtggtta tggtcgtggc cgacgcatgc aaattgagaa agatacagta gaaatagtat
      301 caggcgttag aaatggttat acattaggta gtccaattac tatggttgta accaatgatg
      361 actttacgca ttggagaaaa attatgggag cagctccaat aagtgaagaa gaacgtgaaa
      421 atatgaaacg tactattaca aaaccaagac ctggtcatgc agatttggtt ggaggtatga
      481 aatataatca tcgtgattta cgaaatgtgc tagagcgatc atctgctaga gaaacagcag
      541 ctcgagttgc agtcggtgcc ttatgtaaag tgttattaca acagttagat atcgatatat
      601 acagtcgtgt tgttgaaata ggtggaatta aagataaaga tttttatgat tcaaaaacat
      661 ttaaagcaaa tcttgatcgt aatgatgttc gtgtaattga tgacagtatc gcacaagcaa
      721 tgcgagataa aattgacgaa gctaaaaacg atggtgattc aataggggga gtagttcaag
      781 ttgtagttga aaatatgcct gttggtgtag gtagttatgt acattatgat cgtaaattag
      841 atggaagaat agcacagggt gtcgttagta ttaatgcatt taaaggtgta agttttggag
      901 aaggatttaa agcagctgaa aagcctggta gcgaaattca agacgaaatt ctctacaata
      961 ctgaattggg ctattatcgt gggtcaaatc acttaggtgg tttagaaggc ggtatgtcaa
     1021 atggaatgcc aattatcgtt aatggtgtaa tgaaaccaat tccaacgtta tataaaccat
     1081 taaattcagt agacattaat actaaagaag actttaaagc aacaattgaa cgttctgata
     1141 gttgtgctgt tcctgcagca agtatcgtct gcgaacatgt cgtagcattt gaaattgcga
     1201 aagcattatt ggaagaattc caatcaaatc atattgagca acttaaacaa caaattattg
     1261 agcgcagaca attaaatatt gagttttaac aacaagaaca attgaggtgt aatcatgaaa
     1321 ttacaaacaa catacccttc aaataattat ccaatatatg ttgaacgcgg tgcaattgac
     1381 catattagca cgtatattga tcagtttgat caaagtttta tattaattga tgaatatgta
     1441 aatcaatatt ttgtgaataa atttgatgat attttatcat atgaaaatgt gcataaagtc
     1501 attattccag caggtgaaaa gacaaaaaca tttcatcaat atcaagaaac attagagtat
     1561 attttatccc atcatgtgac tcgtaataca gcaattattg ctgttggtgg tggtgcaact
     1621 ggtgattttg caggatttgt agcagcaaca ctattaagag gtgtccattt tatacaagtt
     1681 cctacaacga ttttggcgca tgattctagt gttggcggta aagtgggtat taactcaaaa
     1741 caaggtaaaa accttatcgg tgcattttat cgtccaactg ctgtgattta tgatttagac
     1801 tttttaaaga cgttaccatt tgagcaaata ttaagtggct atgcagaagt ttataagcat
     1861 gcgttattga atggtgaatc aacgacgcaa gaaatcgaac agcactttaa agatagagag
     1921 atattacagt cattaaatgg tatggataaa tatattgcta aaggtattga aacgaagctg
     1981 gatattgttg ttgcagatga aaaagaacaa ggtgtacgta aatttttaaa tttaggtcat
     2041 acatttgggc atgctgttga atactatcat aaaatacctc atggtcatgc agtgatggtt
     2101 ggcattatct atcaatttat agttgcgaat gctttgtttg attctaagca tgaaattaat
     2161 cattatattc aatatttaat acaactcggt tatcctttag acatgataac tgacttggat
     2221 tttgaaacgt tatacgaata tatgctaagt gataaaaaga atgataagca aggtgtacaa
     2281 atggtcttga ttagacaatt tggagatatc gttgtacaac atgttgatca actaacatta
     2341 caacatgcat gtgaacaatt aaaaacatat tttaagtagg tgaatgaaat ggtaaatgaa
     2401 caaatcattg atatttcagg tccgttaaag ggcgaaatag aagtgccggg cgataagtca
     2461 atgacacacc gtgcaatcat gttggcgtcg ctagctgaag gtgtatctac tatatataag
     2521 ccactacttg gcgaagattg tcgtcgtacg atggacattt tccgactgtt aggtgtagaa
     2581 atcaaagaag atgatgaaaa attagttgtg acttccccag gatatcaatc ttttaacacg
     2641 ccacatcaag tattgtatac aggtaattct ggtacgacaa cacgattatt ggcaggtttg
     2701 ttaagtggtt taggtattga aagtgttttg tctggcgatg tttcaattgg taaaaggcca
     2761 atggatcgtg tcttgagacc attgaaactt atggatgcga atattgaagg tattgaagat
     2821 aattatacac cattaattat taagccatct gtcataaaag gtataaatta tcaaatggaa
     2881 gttgcaagtg cacaagtaaa aagtgccatt ttatttgcaa gtttgttttc taaggaaccg
     2941 accatcatta aagaattaga tgtaagtcga aatcatactg agacgatgtt caaacatttt
     3001 aatattccaa ttgaagcaga agggttatca attaatacaa cccctgaagc aattcgatac
     3061 attaaacctg cagattttca tgttcctggc gatatttcat ctgcagcgtt ctttattgtt
     3121 gcagcactta tcacaccagg aagtgatgta acaattcata atgttggaat caatccaaca
     3181 cgttcaggta ttattgatat tgttgaaaaa atgggcggta atatccaact tttcaatcaa
     3241 acaactggtg ctgaacctac tgcttctatt cgtattcaat acacaccaat gcttcaacca
     3301 ataacaatcg aaggagaatt agttccaaaa gcaattgatg aactgcctgt aatagcatta
     3361 ctttgtacac aagcagttgg cacgagtaca attaaagatg ccgaggaatt aaaagtaaaa
     3421 gaaacaaata gaattgatac aacggctgat atgttaaact tgttagggtt tgaattacaa
     3481 ccaactaatg atggattgat tattcatccg tcagaattta aaacaaatgc aacagttgat
     3541 agtttaactg atcatcgaat aggaatgatg cttgcagttg cttctctact ttcaagcgag
     3601 cctgtcaaaa tcaaacaatt tgatgctgta aatgtatcat ttccaggatt tttaccaaaa
     3661 ctaaagcttt tagaaaatga gggataatat aaaatggaag atatctataa attaatagac
     3721 gatatcaatc tacaaaaact agaaaattta gactctcgtg ttaatgaagc aataactact
     3781 gacaacgatg acgcattatt tattctagga gagacacttt acaattttgg attaatgcca
     3841 caaggtttgg aagtatttcg cgtgttatat cacaaatatc cagacgaaag tgaattgctg
     3901 atttatttta ttgaaggttt aatgtctgaa aatcaaactg acgaagcgtt agaatattta
     3961 agttatgttg aaccatcacc tgaaaagttg atgttagaag cagatttata tcaacaaatt
     4021 aatatgatgg aagttgctat tgctaaatta caagaagcac ttgaactaga gccaaatgat
     4081 ccaataatcc attttgcatt ggctgaaatg ttatattatg atggtcaata tttacgtgct
     4141 acctctgaat acgaaaccgt tttagaaact ggtgaatatc aagttaatgg tgtaaactta
     4201 ttctctcgta tggcagattg tagtttacaa agtggtaact atagtgatgc gattcgctta
     4261 tacgatgaaa ttaatgaaga tgaaatgact tcagaagatt atctcaaaaa agccatttct
     4321 tacgataaaa atgacatcac tcaagaagca attaaaataa tgactacatt actttctaaa
     4381 gatcctgatt atattcaagg ctacttgtat ttacaatcat tatatgaaaa tgaaaaaaat
     4441 tatccagatg ccattgagac gggtaaagaa ggattacgac taagtcaatt ttataaagag
     4501 ttaatgtata caactggatg tttggaaata gaacacggcg atgctaatga aggtgtatta
     4561 ttgttaagac aagcattaga ggttgataat gcttaccaag aacctttatt gattttaagc
     4621 gatttatatc gtaatgaaga agattatgaa gcaatcattg aattattaac atatgtagat
     4681 gaagaagatt tggatcctac attcatgtgg catttagcgt tcgcctatgg tcaagaagag
     4741 cgagataaag aagctcaaca tttctttgaa ttagcatatc caacgatgaa aacaaatatt
     4801 gattttatga gtgattatta tttctattta actgaaattg gtcaaaagga acaagcaatt
     4861 actattttaa atcaattgtt ggaactagag ccaagtaacg aaaattggca tgacgaatta
     4921 tcaagattgc aatcttaaaa tggggtattt gatgtcagag acactcaatc aaattaaaga
     4981 aagttttatt gaatatttac tttttcaata tcgatttaag tcgcgtattg cagtttgggt
     5041 gcttaactat ataaaagtga atgaggcgaa attggcaaac attcattttg tagatacaaa
     5101 aattaatcat catgaaacat tagaaattgc agaagtggga agtcacgctt ctgcaattca
     5161 atttactaag cgtaatataa agttaatgaa tacaaatgaa atatttgatt atatcgctaa
     5221 tcataactgt gcttttgata ttcaaattca cttcgcaaac gtgtcaaagc gtgagcaaag
     5281 gctagatgat ttaatagttg ctcaactaac ggaatcgccc agttatcaaa cttatcttca
     5341 tgacttaaat agtatggcta tagatcggca taaacatgcg ttattaatag attacttgtt
     5401 acacaatatt gatttaagtt tacagatgaa tgaaaagcaa cgtttttatc aactaacgca
     5461 aattttaaat acactcaagc tagtaaataa acataatcaa tttgaggact tagcagatga
     5521 cgattaatac attttggcaa tatacacttt atcaacgttc ttggttaatg atgctactca
     5581 tttgtaatat attagggatg atttatggtt atatatggta tggtgaacaa ttaagtcata
     5641 cgccgtggca ttttaagatt tttgttccag atagcccaac tgcaatatta ttcttagtta
     5701 tttctatctc aatgatatta atccaaaaac aaaatagtat tatcgatgca ctggcatttg
     5761 taacattatt caaatatggg atttgggcag ttattatgaa tatattattt attattgaac
     5821 aaggtgatat tacagtaaat ggcctgatat taatgttttc ccacagtata atggccgttc
     5881 aggcaattta cttctatcct aggtttaaac gttctatgat tggaatcagt gtagcgatga
     5941 cttgggtatt tttgaatgat tatattgact attttcacct tcaatttcca tattatgatt
     6001 ttataactac acatgtgtgg caaattggtg tattatcatg ttgtctgagt gtttttggac
     6061 ttttgcttta tattgaatta aacaaattat taaagtgtaa atgatttgat aaaa
//
LOCUS       DAMZKC010000140         6067 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.142, whole genome shotgun sequence.
ACCESSION   DAMZKC010000140 DAMZKC010000000
VERSION     DAMZKC010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6067
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.142"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            337..1737
                     /locus_tag="POB55_002095"
     CDS             337..1737
                     /locus_tag="POB55_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6501050.1"
                     /translation="MDFNKENINMVDAKKAKKTVVATGIGNAMEWFDFGVYAYTTAYI
                     GANFFSPVENADIRQMLTFAALAIAFLLRPIGGVVFGIIGDKYGRKVVLTSTIILMAF
                     STLTIGLLPSYDQIGLWAPILLLLARVLQGFSTGGEYAGAMTYVAESSPDKRRNSLGS
                     GLEIGTLSGYIAASIMIAVLTFFLTDEQMASFGWRIPFLLGLFLGLFGLYLRRKLEES
                     PVFENDVATQPERDNINFLQIIRFYYIDIFVCFVAVVFFNVTNYMVTAYLPTYLEQVI
                     KLDATTTSVLITCVMAIMIPLALMFGKLADKIGEKKVFLIGTGGLTLFSIIAFMLLHS
                     QSFVVIVIGIFILGFFLSTYEATMPGSLPTMFYSHIRYRTLSVTFNISVSIFGGTTPL
                     VATWLVTKTGDPLAPAYYLTAISVIGFLVITFLHLSTAGKSLKGSYPNVDNEQDRAYY
                     AEHPKEALWWVKERKN"
     gene            2281..3657
                     /locus_tag="POB55_002096"
     CDS             2281..3657
                     /locus_tag="POB55_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HDI6501051.1"
                     /translation="MNVILEQLKTHTQNKPNDIALHIDDETITYSQLNARITSAVESL
                     QKYSLNPVVAINMKSPVQSIICYLALHRLHKVPMMMEGKWQSTIHRQLIEKYGIKDVI
                     GDTGLMQNIDSPMFIDSTQLQHYPNLLHIGFTSGTTGLPKAYYRDEDSWLASFEVNEM
                     LMLKNENAIAAPGPLSHSLTLYALLFALSSGRTFIGQTTFHPEKLLNQCHKISSYKVA
                     MFLVPTMIKSLLLVYNNEHTIQSFFSSGDKLHSSIFKKIKNQANDINLIEFFGTSETS
                     FISYNLNQQAPVESVGVLFPNVELKTTNHDHNGIGTICIKSNMMFSGYVSEQCINNDE
                     WFVTNDNGYVKEQYLYLTGRQQDMLIIGGQNIYPAHVERLLTQSSSIDEAIIIGIPNE
                     RFGQIGVLLYSGDVTLTHKNVKQFLKKKVKRYEIPSMIHHVEKMYYTASGKIAREKMM
                     SMYLRGEL"
     gene            3659..4798
                     /locus_tag="POB55_002097"
     CDS             3659..4798
                     /locus_tag="POB55_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HDI6501052.1"
                     /translation="MNQAVIVAAKRTAFGKYGGTLKHLEPEQLLKPLFQHFKEKYPEV
                     ISKIDDVVLGNVVGNGGNIARKALLEAGLKDSIPGVTIDRQCGSGLESVQYACRMIQA
                     GAGKVYIAGGVESTSRAPWKIKRPHSVYETALPEFYERASFAPEMSDPSMIQGAENVA
                     KMYDVSRELQDEFAYRSHQLTAENVKNGNISQEILPITVKGEIFNTDESLKSHIPKDN
                     FGRFKPVIKGGTVTAANSCMKNDGAVLLLIMEKDMAYELGFEHGLLFKDGVTVGVDSN
                     FPGIGPVPAISNLLKRNQLTIENIEVIEINEAFSAQVVACQQALNISNTQLNIWGGAL
                     ASGHPYGASGAQLVTRLFYMFDKETMIASMGIGGGLGNAALFTRF"
     gene            4773..5138
                     /locus_tag="POB55_002098"
     CDS             4773..5138
                     /locus_tag="POB55_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein VraC"
                     /protein_id="HDI6501053.1"
                     /translation="MQHYLLDSNQRLNVSFSKDSVAAYYQCFNQPYRKEVPPLMCASL
                     WPKFDLFKKYANSELILTKSAINQTQKIEVDTIYVGHLEDIECRQTRNITRYTMALTL
                     TKNDQHVITVTQTFIKAMK"
     gene            5141..5410
                     /locus_tag="POB55_002099"
     CDS             5141..5410
                     /locus_tag="POB55_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501054.1"
                     /translation="MKFNEIWINEYLALVNDDNPIHNEIVPGQLVSQMMLMAMSLETN
                     QCQINYVKPILINENIEFIEQHEHEIIAINDDGEIKIKISLSTKK"
     gene            complement(5611..5778)
                     /locus_tag="POB55_002100"
     CDS             complement(5611..5778)
                     /locus_tag="POB55_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein VraX"
                     /protein_id="HDI6501055.1"
                     /translation="MIIYRQYHHEGAPVYEIITKTFQHVSIKCDDSFSDTEIFKLLSL
                     LQDDIDHMKVS"
BASE COUNT         2121 a          863 c         1117 g         1966 t
ORIGIN      
        1 aataaattaa agtaatgaca aagttattga agtaaattga gtataaacat ttaaatacga
       61 tgtcgaaaat ggcgatagca tatgacttac atgaagttgt gtgctatcgc tatttttagt
      121 tataattcca aaaagttaat cgttcgatga tttaagaatt attattgttt aattcaaatg
      181 tatgagggta taaaatcatt gaatttaatt cgataaagcg aaatttttga acaaacatac
      241 ttttgtattt atataaaagt tttaattctt ataaatttga caaaactaat taactccgta
      301 taattatgaa acatacaaga gggagtgtat gaattcatgg attttaataa agagaatatt
      361 aacatggtgg atgcaaagaa agctaaaaaa accgttgttg caaccggtat cggtaatgca
      421 atggaatggt tcgattttgg tgtctatgca tatacaactg cgtacattgg agcgaacttc
      481 ttctctccag tagagaatgc agacattcga caaatgttga ctttcgcagc attagccatt
      541 gcgtttttat taagaccaat tggtggtgtc gtatttggta ttattggtga caaatatgga
      601 cgtaaagttg tattaacatc tacaattatt ttaatggcat tttcaacatt aaccattgga
      661 ttattgccaa gctatgatca aattggactt tgggcaccaa tactattatt gcttgcaaga
      721 gtactacaag ggttttcaac aggtggagag tatgcggggg caatgacata tgttgccgaa
      781 tcatctccag ataagcgtcg taactcatta ggtagtggac tagaaattgg gacattatca
      841 ggttacatag ctgcttcaat tatgattgct gtattaacat tctttttaac agatgaacaa
      901 atggcatcat ttggttggag aatcccattc ttactcggtt tattcctagg attattcggc
      961 ttatatttac gtcgtaagct ggaagaatca ccagttttcg aaaatgatgt tgcaacacaa
     1021 ccagaaagag ataacattaa ctttttacaa atcatcagat tttattacat agatatattt
     1081 gtatgttttg tagctgttgt attcttcaat gttacaaact atatggtaac tgcatattta
     1141 ccaacctatt tagaacaagt tattaaatta gatgcaacga caacaagtgt attaattact
     1201 tgtgtcatgg caataatgat tccattagca ttaatgtttg gtaagttagc ggataaaata
     1261 ggtgaaaaga aagtatttct aattggtact ggtgggctaa cattattcag tatcatcgca
     1321 tttatgttat tacattcaca atcatttgtt gtaatagtaa tcggtatatt tatattagga
     1381 tttttcttat caacttacga agcgacaatg ccagggtcgt taccaacgat gttttacagt
     1441 catataagat atcgaacttt atcagtaaca tttaatatct ctgtttcgat atttggtggt
     1501 acgacgccat tagttgcaac atggttagtt acgaaaactg gagatccatt agcacctgcg
     1561 tattatttaa cagcaatcag tgttattggc tttttagtta ttacattctt acatttaagt
     1621 acagcaggaa aatctctaaa aggttcgtat ccaaatgtag ataacgagca agatagagct
     1681 tattatgcag aacatccaaa agaagcatta tggtgggtta aagaacgtaa gaattagaga
     1741 ttttaataaa aagtataaat caatcgtata taagcacttt aaagctagta ggttctgcta
     1801 actttaaagt gctttttaaa ttgagaactg taattagccg taataaagtt tttgtatata
     1861 cataaacccc cactgcaatg attatcgcaa tgggggaaag aggggactta aagcatatgt
     1921 ttagctttga atacttaaaa ttctcttgct attgaaatgt taggatgtaa atatgtctta
     1981 gagtattttg tccaacgcaa ttaatattga gactctaacc ttcaatatta ttatagagaa
     2041 cacaaactta aatagattgg gtgacttatt tgtgtcagtt attgcgattg cgataacttc
     2101 ttttctctat atacatatag taacgtctta tctaataaaa aacatggtac tacagtatca
     2161 aatttatcta gggcttaagt ttgattttta taataggcag gtttacctga taaaaatact
     2221 tattcattat ataatgttaa caatatgtat tttaaagttt acattgagtg agggatattg
     2281 atgaacgtaa ttttagaaca gttgaaaaca catactcaaa ataaacctaa tgacatagca
     2341 ttacatatcg atgatgaaac aattacatat agtcaactaa atgcccgcat cactagcgca
     2401 gttgaatctt tgcagaaata ttcacttaac cctgtcgttg ctattaatat gaaatcaccg
     2461 gtgcaaagta ttatttgtta tttagctttg catcgtttac ataaagtgcc tatgatgatg
     2521 gaaggtaaat ggcaaagtac tatacatcgt caattgattg aaaaatatgg tattaaagat
     2581 gtaattggag atacaggtct catgcagaat atagactcac cgatgtttat tgattcaacg
     2641 caattacagc actaccccaa tttattacat attggtttta cttcagggac aactggactg
     2701 ccaaaagcat attatcgtga tgaagattca tggttggctt cttttgaagt taatgaaatg
     2761 ttgatgttaa aaaatgaaaa tgcaatagca gcccctggac cactatcgca ctcgttaaca
     2821 ttatatgcgt tattgtttgc tttaagttcc ggtcgtactt ttataggaca gaccactttt
     2881 catcctgaaa agttacttaa tcaatgtcat aaaatatcat catacaaagt tgctatgttt
     2941 cttgttccaa cgatgattaa atcattattg ttagtttaca acaatgaaca tacaatccaa
     3001 tcatttttta gcagtggaga taagctgcat tcttctattt ttaaaaagat aaaaaatcaa
     3061 gcaaatgaca taaatttgat tgaatttttt ggtacatcgg aaaccagttt tatcagctat
     3121 aacttgaatc agcaagcacc agttgaatca gtaggtgtgc tatttccaaa tgtggaattg
     3181 aaaacaacga atcacgatca caatggtata ggaactattt gtataaaaag taatatgatg
     3241 tttagtggct atgtaagtga acaatgtata aataatgatg aatggtttgt tactaatgat
     3301 aatggctatg taaaagagca gtatttatat ttaacgggac gtcaacagga tatgttaatt
     3361 attggtggtc aaaatatata tccagcacat gttgaacgcc ttttaacgca atcttcgagc
     3421 attgatgaag caattatcat cggtattcca aatgagcgtt ttggtcaaat aggcgtattg
     3481 ctttattctg gtgatgtgac acttacacat aaaaatgtaa aacaattttt aaaaaagaaa
     3541 gtgaaacgct atgaaattcc atcgatgatt catcatgtag aaaagatgta ttacactgca
     3601 agtggtaaaa ttgctagaga aaaaatgatg tcgatgtatt tgagaggtga attataatat
     3661 gaatcaagca gtcatagttg cagctaaacg aactgcattt gggaaatatg gtggcacttt
     3721 aaaacattta gagccagaac aattgcttaa acctttattc caacatttta aagagaagta
     3781 tccagaggta atatctaaaa tagatgatgt agttttaggt aatgttgttg ggaatggtgg
     3841 caatattgca agaaaagcat tgcttgaagc ggggcttaaa gattcaatac ctggcgtcac
     3901 aatcgatcgg caatgtgggt ctggacttga aagtgttcaa tatgcatgtc gcatgatcca
     3961 agccggagct ggcaaggtat atattgcagg tggtgttgaa agtacaagtc gagcaccttg
     4021 gaaaatcaaa cgaccgcatt ctgtgtacga aacagcatta cctgagtttt atgagcgtgc
     4081 atcatttgca cctgaaatga gcgacccatc aatgattcaa ggtgctgaaa atgtggccaa
     4141 gatgtatgat gtttcaagag aattacaaga tgaatttgct tatcgaagtc atcaattgac
     4201 agcggaaaat gtaaagaatg gaaatatttc tcaggaaata ttacctataa ccgttaaagg
     4261 agaaatattc aacactgatg aaagtctaaa atcacatatt ccgaaagata actttggccg
     4321 atttaagccc gtgatcaaag gtgggaccgt taccgctgcg aatagttgta tgaaaaatga
     4381 tggtgcagtt ttattgctta ttatggaaaa agatatggca tacgaattag gtttcgagca
     4441 tggtttatta tttaaagatg gtgttacggt aggtgttgat tctaattttc ctggcattgg
     4501 tccagtacca gccatttcca acttactaaa aagaaatcaa ttaacgatag aaaatattga
     4561 agtcattgaa attaacgaag cgttcagtgc acaggtagtt gcctgccaac aagctttaaa
     4621 tatttcaaat acgcaattaa atatatgggg tggtgcatta gcatcaggtc atccatacgg
     4681 tgcaagcggt gcccaattag tgactcgatt attttatatg tttgacaaag agactatgat
     4741 tgcatctatg gggatagggg gaggtctagg aaatgcagca ttatttactc gattctaacc
     4801 agcgattaaa tgtgtcattt tctaaggata gtgtggctgc atattatcag tgttttaacc
     4861 aaccttatag aaaagaagta ccaccattaa tgtgtgcgtc attatggcca aaatttgatt
     4921 tatttaaaaa atatgcaaat agcgaactga ttttaacaaa atcagcaatt aatcaaactc
     4981 aaaagataga agtagacaca atatatgtag ggcatttaga agatattgaa tgccgacaga
     5041 ctcgcaatat cacacgttat acaatggctt taacattaac taaaaatgat caacatgtca
     5101 taacggttac acaaactttt attaaggcga tgaagtagag atgaagttta atgagatatg
     5161 gataaatgaa tatttggcgc tcgtaaatga tgataatcca atacataatg agattgtgcc
     5221 aggacaatta gtgagtcaaa tgatgctgat ggctatgtca ttagagacaa accagtgtca
     5281 aattaactac gttaaaccta ttttaataaa tgaaaatatc gaattcattg aacaacacga
     5341 acacgaaatt atagcaatta atgacgatgg agagattaaa ataaaaattt ctttgagcac
     5401 aaaaaaataa ccgatattag ctgcatgaac gcatattaat taggagatga aaggacagct
     5461 aatatcagtt atgtattgtt attattattg ggaacagaga tgaatatagg ttacgtttct
     5521 ttctttgcac ggggatgcat taatctaaaa taataataac aactatatca atgtttaata
     5581 aattctggat tattggaacg attagtcaat ttaactaact ttcatatgat ctatatcgtc
     5641 ttgtaataaa gagagcaatt tgaatatttc agtatcacta aatgaatcgt cacatttaat
     5701 tgaaacatgc tgaaacgttt tggttataat ttcataaact ggtgcgcctt catggtgata
     5761 ctgtcgataa ataatcataa cctatattac ctcctttgct actctatggt tatattataa
     5821 ataacatttt tatgtgtgac atcaacctta agtatcaact ttttatcaga catagaacgt
     5881 atgatttact aagactattt atgtataaaa gttctaaata aatatatatt tatagagtcg
     5941 cctggcagtc atttgggaaa tataacatat atgattagag aggcatctat cgcaaaagaa
     6001 tgataatgat agaggtattg agcatataga tgagtttaag ttcatcttga aaataaaggg
     6061 ttattta
//
LOCUS       DAMZKC010000141         6056 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.143, whole genome shotgun sequence.
ACCESSION   DAMZKC010000141 DAMZKC010000000
VERSION     DAMZKC010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6056
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.143"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(<1..72)
                     /locus_tag="POB55_002101"
     CDS             complement(<1..72)
                     /locus_tag="POB55_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4865 family protein"
                     /protein_id="HDI6501056.1"
                     /translation="MHAMQYHVKLPSDYNMEIIRDRVR"
     gene            163..906
                     /locus_tag="POB55_002102"
     CDS             163..906
                     /locus_tag="POB55_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI6501057.1"
                     /translation="MIKLNFNDLEIFITVCEEASINKAAIKLRYAQSNISQRISKLEN
                     ELGVVLLFRNQKGAKATKAGEEFLAYSKKVLRDTETIKNKMKNNTMSILCSELLFNYL
                     SESEEIMMSNNSINFISSGNIRKAIEKNNYDKVISFIKINDSNYRLSNVDTMKVTLYS
                     NGSNYDKEALLINKDEFCPLRKITLDNKLDSQRVMEIDSLAAIINLVKQGKGKALLPM
                     TFENKRDIVQDISKIFEVNYYTYNHIMHH"
     gene            1340..1942
                     /locus_tag="POB55_002103"
     CDS             1340..1942
                     /locus_tag="POB55_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501058.1"
                     /translation="MLISLVIPVFALLVIGGIIWMIIEGIVHISKKNKAIDNFFNQVN
                     KVSETYKFATTFLFLILATAGISQFYLYYIVSALLFWLVILTFGIAGIIFLMPYGLCF
                     LPFYKQKKKKQTFKKYMVYTTIGLSICLGLSLVLVHTTKIYMDEGGVRYYYGSFVMKQ
                     AGGYAYLALAVLSTLLIVAKKATNKNKEIETVDNTNIMER"
     gene            2209..5361
                     /locus_tag="POB55_002104"
     CDS             2209..5361
                     /locus_tag="POB55_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA2/NAM7 family helicase"
                     /protein_id="HDI6501059.1"
                     /translation="MSQLLNDTLSAWLLIESLSPGEVNFTAEDILSAEHFKNGAKQAQ
                     LQSFDEYFEIWNAERFIISEEKSETGELIFKFYRHCFRYNEINLKIQDIFDDYSEIHN
                     PNGTHCYGYTFNTDKHGKVIVDSIHIPMIMSALKEIEKNKNANIEEKFNDSVEKFVQK
                     VKEILADEPINEFKLKKMDKAYDEYFSVLNSKKDGLFGHYVAIEYVKDSDLPQPEFNS
                     FFISDIEKARKSPNQTLIDYIEGVEESQRIEVDENKEMFDKFLHPSRLPDGRWPSQTE
                     FRLSLMQQLAVNQITSGNERISSVNGPPGTGKTTLLKDIFAHLVVERGKELAKLNNPK
                     DAFVKTKIHETDDKYVYLLKESIAKYKMVVASSNNGAVENISKDLPKIEEIIRNPEKC
                     KFPKYEQNYANLAHELKDFAEIAEDLIGESAWGLFSGVFGKSTNINQVLSHMLKQDAN
                     DIGFAKLLQNENNRMSYNELMSEWQSHQRAFLEELRHVEMLKEESIRAYDVYKNCESF
                     SKIEQVINSEKTSIEEQVYHLDNETLRDNKEIEDLDNRINYIVKQIETLNELIKSIKE
                     SNKGFINKLKAMFNSEEDESYKDHNKEKQQLLTQQLELEKCKKNKHEDLVSKLKEKEK
                     LIKQLTKVQLQLDELNSQLQELEAYRIESKITIPEKDFWSDNNYDERQVTNLWTSDEL
                     QYRRAMLFLRAMILHKLLLIANNTTIYYAINDFKDRRKLIDANPDKVHNAWNVMHLIF
                     PVVSTTFASFKSMYGGIPKDFIDYLFIDEAGQAIPQAAVGALYRSKKVVAVGDPIQIE
                     PVVTLESHLIDNIRKNYHVPEYLVSKEASVQSVADNANQYGFWKSDATDSNQKTWIGI
                     PLWVHRRCLKPMFTIANQIAYNNKMVLPSNITKVGKTGWYDVKGNAVQKQFVKEHGEK
                     VVGLLADDWIEAIKEGKNEPSSFVISPFSAVQQQIKRMLKQQLPTRIDIERTKINQWV
                     DKSIGTVHTFQGKEAQKVYFVIGTDNTQDGAVNWSCEKPNLLNVAVTRAKKEFYVIGD
                     MQRIQMKPFYETIFKERNVK"
     gene            5418..5900
                     /locus_tag="POB55_002105"
     CDS             5418..5900
                     /locus_tag="POB55_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501060.1"
                     /translation="MEKFNNWILNAISGSQTDKNETTEELKGAKFIILYAYSMLVLLA
                     LVISNIFIHILEPKLSITTQIIIVLILIEALIGLRFLKAYDVKRGKDKENKKNSKDFV
                     KLKSILVAILFTSLALTAGTVADIYGFTDLGNTRSDLIVWSIGGIIFGLVCYTMEDKR
                     "
BASE COUNT         2337 a          737 c         1061 g         1921 t
ORIGIN      
        1 tcgaactcta tctctaatta tttccatatt gtaatcacta ggcaacttaa catgatattg
       61 cattgcatgc atttagcata ccccctttta taaaaaggat agcaataata agtaaaatct
      121 catattatcc aattgtgata tagttatcat aaaaagtgat aggtgattaa attgaacttt
      181 aatgatttgg aaatttttat aactgtatgt gaagaagcat ctatcaataa agctgcaatt
      241 aaacttagat atgcacaatc taatatatct caaagaatta gcaagcttga aaatgaatta
      301 ggtgtagttt tgctttttag aaatcaaaaa ggtgctaagg caactaaagc aggcgaagaa
      361 ttcttagcgt atagcaaaaa agtattaaga gatacagaga ctataaaaaa taaaatgaaa
      421 aataatacta tgtctatttt atgctcagaa ctgttattta attatttatc tgagagcgaa
      481 gaaattatga tgtcgaataa ctcaattaat tttatttcta gtggaaatat tagaaaagct
      541 atagaaaaaa ataattatga taaggttatt tcattcataa aaattaacga ctcaaattat
      601 agacttagta atgttgatac tatgaaagta acgctttaca gtaatggaag taattatgat
      661 aaagaggctt tactaataaa taaagatgag ttttgtcctt taaggaaaat aactttagat
      721 aataagcttg attcacaacg ggtaatggaa attgattcat tggcagcaat aataaactta
      781 gttaaacaag gaaaaggaaa ggctttatta cctatgactt ttgagaataa aagagatata
      841 gtacaggaca tttctaagat atttgaagtt aactactata cttataatca tattatgcat
      901 cattaattga aagttgattg agtttaaatt acaggaacga ttttaaaagt ctagtagtta
      961 gaatgagcga tgtgacagaa aagaaattat gccttttatt ttgtaaattc gttgttttaa
     1021 agtaaaggtc cttgtagtat aggaaaaagt ggatgacttc tccgccttta ccaccctttc
     1081 aagcataatg ataactgcta aatattgatt atagcaccat tgcaaaacta atcattaatt
     1141 gtcatattca actattttga aaagtatcaa tttcacatta tcaaattatt gagtatctca
     1201 atttattgtt atacacaaac agtatttttg ttatcataga gttaataaat ataataaatt
     1261 aaacaagtaa ctttacaaaa attagaatga ttcaattcaa tcataattgt ttgaaaatag
     1321 aggtgagcag gtgaacgata tgttaattag tcttgtaatc ccagtttttg ctttgttagt
     1381 tattggtggt attatttgga tgattataga aggtatagta catatttcaa aaaagaataa
     1441 agcaattgat aactttttta accaagttaa taaagtaagt gagacatata aattcgctac
     1501 tactttttta tttctaatct tggctacggc gggtatttct caattttatc tatattatat
     1561 agtatcagcg ttactttttt ggcttgtcat acttaccttt ggtattgcag gtatcatttt
     1621 tttaatgcca tatggattat gttttctacc gttttataag caaaaaaaga aaaaacagac
     1681 atttaaaaaa tacatggttt acactacgat tggtttgtca atttgtctag gcttatctct
     1741 agttttggtt cacactacga aaatttatat ggacgaaggt ggcgtaagat actattacgg
     1801 tagttttgta atgaaacaag cgggcggtta tgcttattta gctttagcag tactttcaac
     1861 gttgttaatt gttgcgaaaa aagctacaaa taaaaataaa gaaatcgaaa ccgtcgacaa
     1921 tacaaatata atggaaagat aattaaggga gtgctcattc aggagtgctc ttttttgatg
     1981 ttcaaattta gttggaaatg aaggcataga agaaattttt atagtcattt attggataat
     2041 gaattatggt catatcgatt aattttatta gtgaggattt tacaaacatt tgttgaataa
     2101 atatggtaat gataatatat gaatattttt gaaaaaacac taatatgatt aaattatttg
     2161 ttaaaatggc attaagtaaa aaacaaacag ggaacaaggt gggatttcat gagtcaattg
     2221 ctaaatgata cgttatcggc ttggttgtta attgaatctt taagtccagg agaagtaaat
     2281 tttacagcgg aagatatact ctcagctgaa cattttaaaa atggtgcaaa gcaagcacaa
     2341 cttcaaagtt ttgatgaata ttttgaaata tggaatgctg aacgctttat tatatcagaa
     2401 gaaaaatcag agactgggga acttatattt aaattttata gacattgctt ccgctataat
     2461 gaaattaatt tgaaaattca ggatattttt gatgattatt ctgagattca taatccaaat
     2521 gggacacact gttatggtta cacatttaac acagataaac acggcaaagt gatagttgat
     2581 tctatacata ttccgatgat tatgagtgca ttaaaagaaa ttgaaaagaa caaaaatgcc
     2641 aatatagaag aaaaatttaa tgattctgtt gaaaaatttg ttcaaaaagt aaaagaaatt
     2701 ttagcagatg aaccaattaa tgaatttaaa ttgaagaaga tggacaaagc ttatgatgag
     2761 tacttttctg tattaaattc aaagaaagat ggattatttg gacattatgt agcaatagaa
     2821 tatgtgaaag atagtgattt accacagccg gaatttaaca gcttcttcat aagtgatatt
     2881 gagaaagcaa gaaaatctcc caaccaaact ttaattgatt acattgaagg tgtagaagaa
     2941 agtcagcgca tagaagtaga tgaaaataaa gaaatgtttg acaaattttt acatccttca
     3001 cgtttgcctg atggacgatg gccatcacag actgagttta gattgtcttt aatgcaacaa
     3061 cttgctgtaa accaaattac gagtggtaat gaaagaataa gttcagttaa tgggccacca
     3121 ggtacaggta agactacttt attaaaagat atatttgctc atctagtagt tgaaagaggt
     3181 aaagagttag ctaaactaaa taatcctaaa gatgcatttg tcaaaacaaa aattcatgaa
     3241 acggatgata aatacgtata cttactaaag gaatctattg ccaaatataa gatggtagtc
     3301 gcatctagta ataatggagc tgttgaaaat atatctaaag atttaccgaa aattgaagaa
     3361 attataagaa atcccgaaaa atgtaaattc cctaaatatg aacagaatta tgcaaattta
     3421 gcacatgaat taaaagattt tgctgaaata gctgaagatt tgattggtga aagtgcctgg
     3481 ggcttatttt ctggagtttt tggtaaaagt actaatatta accaagtatt gagtcatatg
     3541 ttaaaacaag atgcgaatga tattggcttt gctaaattac tacaaaatga gaataatcgt
     3601 atgagttata acgagttaat gagtgaatgg caatcacatc aacgtgcatt tttagaagag
     3661 ttgaggcatg ttgaaatgtt aaaagaagaa tctattagag catatgatgt ttataaaaat
     3721 tgtgagtctt tctctaagat tgaacaggtt attaatagtg aaaaaacaag tattgaagaa
     3781 caggtatatc atttagataa tgaaacgtta cgagacaata aagaaataga agatttggat
     3841 aatcgaatta attatattgt taagcaaata gaaactttaa atgagttaat taaatccatc
     3901 aaagaaagca acaaaggttt tattaacaaa ctgaaagcga tgtttaattc agaagaagat
     3961 gaaagctata aagatcataa taaagagaag caacaattat taacacaaca gttagagtta
     4021 gagaaatgta aaaaaaacaa acatgaagac cttgttagca aactaaaaga aaaagagaaa
     4081 ttaattaaac aattaactaa agtacagttg caattagacg agttaaattc acagttacaa
     4141 gagttagaag catatcgtat tgagtcaaaa attacaattc cagaaaaaga tttttggagt
     4201 gacaacaatt atgatgagcg ccaagttact aatctgtgga cgagtgacga acttcaatac
     4261 agacgtgcca tgctcttttt aagagcaatg atattgcata aattattatt gattgctaat
     4321 aatacaacta tttattatgc gattaatgat tttaaagata gaaggaaatt aattgatgca
     4381 aatccagata aagtacacaa cgcatggaat gtgatgcatt taatatttcc agtagttagt
     4441 acgacgtttg caagctttaa atctatgtat gggggcatac caaaagattt catagactac
     4501 ttatttattg atgaagcagg acaagcaata cctcaagcag ctgtgggagc attatatcgt
     4561 tcaaaaaaag ttgtagctgt aggtgatccg attcaaatag aaccggttgt gactttagaa
     4621 agtcatttaa ttgataacat tcgtaaaaat tatcatgttc cggaatatct agtttctaaa
     4681 gaagcttctg tgcagtctgt tgcagacaac gccaatcaat atggtttttg gaaatctgat
     4741 gctactgata gtaatcaaaa aacctggata ggcatacctt tatgggtgca cagacgatgt
     4801 ttaaaaccta tgttcacgat agctaaccaa atcgcttata ataataaaat ggtgttgcca
     4861 agtaatatta caaaagtagg taaaacaggt tggtatgacg ttaaaggaaa cgcagttcaa
     4921 aaacaatttg tgaaagagca tggtgaaaaa gtagtgggat tattagctga tgattggatt
     4981 gaagcaatta aggaaggtaa aaatgaaccg agctcatttg taatatcgcc tttttcagca
     5041 gtacagcaac agattaaacg tatgttaaag caacaactac cgactagaat tgatattgaa
     5101 cgtacaaaaa ttaatcaatg ggtcgataaa tccattggta ctgttcatac ttttcaaggt
     5161 aaagaggctc agaaggtgta ttttgtaata ggtactgata atacccaaga tggtgctgtg
     5221 aactggtcat gcgaaaaacc aaacttgtta aacgttgcag tgacaagagc taagaaagag
     5281 ttttatgtaa ttggcgacat gcaaagaata cagatgaaac cattttatga gacgattttt
     5341 aaagaaagaa atgtaaaata acatacaaaa aagtatatag aggaagttat acattttaaa
     5401 aggagcaaaa ttgaataatg gagaaattta acaactggat attaaatgca ataagtggat
     5461 ctcaaacaga caagaatgaa acaactgaag aattaaaagg ggcaaaattt atcattttat
     5521 atgcatattc aatgctcgtt ttgcttgcgt tagtaatttc taacatattc attcacattt
     5581 tggagcctaa actatcaatc accactcaaa tcatcatcgt tttgatttta attgaagcac
     5641 taattggact gcgtttcttg aaagcgtacg atgttaagcg tggcaaagat aaagaaaata
     5701 agaaaaatag taaggatttc gttaaactaa aatcaatttt agtagcaatt ttatttacat
     5761 cattggcgct gacagcaggt actgtagctg atatatacgg tttcactgac ttaggaaata
     5821 ctagaagtga tttaatcgtt tggagcatag gtggtattat atttggcctc gtatgttaca
     5881 caatggaaga taaaagataa cgataaggag ctggcgatta taaagctagc tcctttttta
     5941 acttatatat gtaaagaact atcctaaggg tttttaatca tatgtcaata atttctataa
     6001 tacattatta aaacatcaat taaataagtt ttaaaatttt acacatattt ttattt
//
LOCUS       DAMZKC010000142         5911 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.144, whole genome shotgun sequence.
ACCESSION   DAMZKC010000142 DAMZKC010000000
VERSION     DAMZKC010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5911
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.144"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..905
                     /locus_tag="POB55_002106"
     CDS             <1..905
                     /locus_tag="POB55_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDI6501061.1"
                     /translation="YILLIIHYFIICYFVFSVHPMLSLFFFYSAFAVPFTFKNNVKKT
                     ATNLFILTMIICTIITYLLYNNYFVAMMVYYVVISLIMLDNFKKMKNREYQKEIAEKN
                     RHINTLIAEQERHRIGQDLHDTLGHVFASLSLKSELAYKLIDADVEKVKAELLAINKL
                     SRESLNKVREIIDDVKLPSFIEEIDSIRKVLKDADIDFTFENKELAQVLSPTKQSMLV
                     MITREAINNVIKHANASKVHGKLKTVNNHKLLLMIEDDGKGIDSDCEVKSISQRVQHL
                     NGTLAVDSTNGTKIIIEISTGGIA"
     gene            902..1504
                     /locus_tag="POB55_002107"
     CDS             902..1504
                     /locus_tag="POB55_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDI6501062.1"
                     /translation="MTSLIIAEDQNMLRQAMVQLIKLHGDFEILADTDNGLDAMKLIE
                     EYNPNVVILDIEMPGMTGLEVLAEIRKKHLNIKVIIVTTFKRPGYFEKAVVNDVDAYV
                     LKERSIEELVETINKVNNGEKEYSATLMTSFFVDKNPLTPKEQIVLREIGNGLSSKEI
                     SEKLFLTDGTVRNYTSVIIDKLFADNRFDAWKKANEKGWI"
     gene            complement(1617..1805)
                     /locus_tag="POB55_002108"
     CDS             complement(1617..1805)
                     /locus_tag="POB55_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HDI6501063.1"
                     /translation="MNISNENTVTLIAVIIAIIIGIFLQIFFKLPLIVTAVLSILLGI
                     FVGFIVYLIVSFYNKRKN"
     gene            1944..2477
                     /locus_tag="POB55_002109"
     CDS             1944..2477
                     /locus_tag="POB55_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HDI6501064.1"
                     /translation="MKSNKSLAMIVVAIIIVGVLAFQFMNHTGPFKKGTNHETVQDLN
                     GKDKVHVQRVVDGDTFIANQNGKEIKVRLIGVDTPETVKPNTPVQPFGKEASNYSKKT
                     LTNQDVYLEYDKEKQDRYGRTLAYVWISKDRMYNKELVEKGLAREKYFSPNGKYRNVF
                     IEAQNKAKQQKLNIWSK"
     gene            complement(2620..3474)
                     /locus_tag="POB55_002110"
     CDS             complement(2620..3474)
                     /locus_tag="POB55_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501065.1"
                     /translation="MSFYVVLIIIIVALIGILVLNQRYSNSKIDTEVYARKQLIKKNK
                     ALSAENAELRSQMLSSNNDVGHHAYKNAKRELRKILDSYLENGKLKYYDIIVTSNLAT
                     KHPFFEYARSFDFIIVSDIGLINVDVKSWGEKTFYHFDVPDEHDTEISNSNIEKVVGH
                     YISQQYHDQFNSSRKSIYTFTETVQPNRVIYDFYDYDPYQLAANNAKALKDHIEQNFN
                     FKVQSTGVIYFSDGTVNIIQGSEERDKYVDTVSTKSSLRRIISEAIELSKHPLNKEQV
                     DQITAIFK"
     gene            3805..4380
                     /locus_tag="POB55_002111"
     CDS             3805..4380
                     /locus_tag="POB55_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501066.1"
                     /translation="MNAKEQLVDNLMKTSSQLFKFHGEVAMQLFLNDELKLPSIVEIC
                     VERKRLSDIVKVIPQSYALLYIDKQDQAIAKEDLSLSKIAKVYVQYDDTTIMSIFVYD
                     VVNDEWIFRLDPNIRIPKSNIYFHSLNWDVDYIKPEIVLMYDLMQHHQYHHYSNYKRV
                     IDALSYYQFFILKFVVGEQRIKDAIQRTINN"
     gene            complement(4434..5816)
                     /locus_tag="POB55_002112"
     CDS             complement(4434..5816)
                     /locus_tag="POB55_002112"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HDI6501067.1"
                     /translation="MKVSKFGGSSVSNATQIKKVLNIVNSDPERKIVIVSAPGKRHDN
                     DIKTTDLLIRLYEKVINHLDYHDKKREIIQRYDDIVKELQMDESILRTIDVTLEHYIN
                     QLKNEPKRLLDALLSCGEDFNAQLIALYNNSQGIPTKYISPKEAGIFVTDVPQEAQIL
                     DSAYSEIKKLRDYDEKLIIPGFFGVSHEGYIVTFPRGGSDITGAIISSGVRATIYENF
                     TDVSGIYKANPNIIKDPELIEEITYREMRELSYAGFSVFHDEALQPLYKDRIPVVIKN
                     TNRPQDKGTFIVHDREINAKNVISGISCDKDFTVINIKKYLMNRQVGFTRKILGVLED
                     NNISFDHMPSGIDTISIVMRSKQIQNKETKVLNEIRQKCDVDELSIEHDLAILMIVGE
                     GMNKVIGTANKITHALAESKINLKMINQGASEISMMFGIHVDDAEKAVLSTYEFCYHG
                     VALKNLCDKN"
BASE COUNT         2122 a          807 c          919 g         2063 t
ORIGIN      
        1 tttacatttt gttgattatt cattatttta ttatttgtta ttttgttttc agtgtacatc
       61 caatgctaag tttgtttttc ttttatagtg cttttgccgt tccatttact tttaaaaata
      121 atgttaaaaa aacggcaact aatcttttca tactaacaat gattatatgt acaataataa
      181 cgtacttatt gtataacaac tattttgttg caatgatggt ttattatgtc gttatatcgt
      241 taataatgct agataatttt aaaaaaatga aaaaccgtga atatcaaaaa gaaatagcag
      301 aaaaaaatag acatattaat acattaattg ctgaacaaga gcgacataga attggtcaag
      361 acttacatga tacgttaggg catgtgtttg caagtttatc attaaaatca gaattagctt
      421 ataaactaat agatgctgat gtagaaaaag taaaagctga attattagca attaataaat
      481 tatctcgtga atcattgaac aaagttcgag aaattattga tgatgtaaaa ttaccatcat
      541 ttattgaaga gattgatagt atacgtaaag ttttaaaaga tgctgatatt gattttacat
      601 ttgaaaataa agaattagcg caagtattaa gtcctactaa acaatctatg ttagttatga
      661 ttacgcgtga agcgataaat aatgttatta aacatgcaaa tgcttcaaaa gttcatggta
      721 aattaaaaac tgtaaacaat cataaattac tgcttatgat tgaagatgat ggcaaaggta
      781 tcgatagtga ttgtgaggtg aaaagtattt cacagcgtgt acaacattta aatggaactt
      841 tagcagtcga ctcaacaaat ggaactaaaa taatcattga aatctcaaca ggaggaatag
      901 catgacatct ttaattattg cagaagatca aaatatgtta cgacaggcaa tggttcaatt
      961 aattaaacta catggtgatt ttgaaatttt agcagatact gataatggtc tcgatgcaat
     1021 gaaacttatt gaagaatata atcctaacgt tgttatttta gatatagaaa tgccaggcat
     1081 gactggactt gaagttttag cggaaattag aaaaaagcat ttgaatatta aagtgattat
     1141 tgtaacaact tttaaaagac cgggatactt tgaaaaagca gttgtgaatg atgtggatgc
     1201 atatgtttta aaagaacgtt ctatagaaga attggtggaa accattaata aagtaaataa
     1261 cggagagaaa gaatatagcg ccacattgat gacttcattt tttgtagata aaaacccatt
     1321 aacgcccaaa gaacaaattg tattaaggga aattggcaat ggtttaagta gtaaagaaat
     1381 aagtgaaaaa ttatttttga cagatggaac agttagaaat tatacatctg ttataattga
     1441 taaattattt gcagataatc gttttgatgc ttggaaaaag gcaaatgaaa aaggctggat
     1501 ctaaatacaa acaaaaaagt attgagtgtt ttgatttaag agacttggaa agtgagtcaa
     1561 taatcaaaat cttactcaat acttttattt tggtactcgc aatttagatt tgttaactaa
     1621 ttttttcgct tattataaaa cgaaacaatc aagtaaacga taaagcctac aaagataccc
     1681 aataaaatag atagtactgc cgtcactatt aatggtaatt tgaaaaatat ttgtaggaaa
     1741 ataccaatga taattgcgat aattactgca attaatgtga ctgtattttc atttgaaatg
     1801 ttcatttatt ttcactcctt aacaataaca ttatatcatg ctatagcttt ccaaaatatt
     1861 gaaatatgta gatatggcta ttgacgatat ttcttaactt ttatatgatt aatcggaatg
     1921 aaaaaagaga agtaggtggc aatatgaagt caaataaatc gcttgctatg attgtggtag
     1981 ccatcattat tgtaggtgta ttagcatttc aatttatgaa tcatacgggt cctttcaaaa
     2041 aggggacgaa tcatgaaact gtacaagatt taaatggtaa agataaagta catgttcaaa
     2101 gagttgtgga tggtgataca tttattgcaa atcaaaatgg taaagaaatt aaagttaggc
     2161 ttataggggt tgatacgcca gaaacggtga aaccgaatac gcctgtacaa ccatttggca
     2221 aagaagcatc aaattatagt aagaagacat taacaaatca agatgtttat ttagaatatg
     2281 ataaagaaaa acaagatcgc tatggtagaa cattggcgta tgtatggata agtaaagatc
     2341 gtatgtacaa taaggaatta gtggaaaagg gacttgctag agagaagtat ttttcaccaa
     2401 atggcaaata tagaaatgta tttatagaag cacaaaataa agctaaacaa cagaaattaa
     2461 atatttggag taaataatta aataggtgat tatatatatt aaagtaaaat atacttgaag
     2521 catcgattta ttattaccat aaatttatat atagaaataa tttaaaggca taataaccat
     2581 agtgcgtatc gagcgtaatg gattattatg cctttgattt tacttgaata tagctgtgat
     2641 ttgatcaacc tgttctttat ttaaagggtg tttagatagc tctatggctt cgctaataat
     2701 acgtcgtaac gaagattttg ttgaaactgt atcaacatat ttatctcttt cttcagaacc
     2761 ttgaatgata ttaactgtac catcactaaa gtaaataaca ccggtacttt ggactttaaa
     2821 gttaaagttt tgttcaatat gatcctttaa tgctttcgca ttatttgctg ctaattgata
     2881 cggatcataa tcataaaaat cataaataac acgatttggt tgaactgttt ctgtaaaagt
     2941 atagatagac tttcttgatg aattaaactg atcatgatat tgctggctaa tgtaatgacc
     3001 gacaactttt tcaatattac tattgcttat ttctgtatca tgctcatcag gtacatcaaa
     3061 gtgataaaat gttttttcac cccagctttt tacatcaacg tttattaaac cgatatctga
     3121 aacaatgata aaatcaaatg aacgtgcata ctcgaaaaac ggatgtttag tagctaaatt
     3181 gcttgtaacg ataatatcgt aatattttaa tttaccattt tcgagatagc tatctaaaat
     3241 cttgcgtaat tctcttttag catttttgta tgcatggtgg ccaacatcat tattagaact
     3301 cagcatttga cttcttaatt cagcattttc tgcacttaat gctttgttct ttttaataag
     3361 ttgctttctt gcataaactt cggtatctat tttactatta ctataccttt gatttaaaac
     3421 taatatacca attaatgcta caatgataat gataagtaca acataaaaag acattttttc
     3481 accaatcctt tttgacttct ttaactttgt atacaataat aattaataaa gattaattgt
     3541 tattcaattt cccacatttt tattagttga ttttagttca tcattgttat aatcaaatta
     3601 taaactgaca gatattgatg ttcaatgaat atgacgtgaa agattcgtga attcaagttt
     3661 atgtcgaatt tatgttataa cggtcattta aatgacagaa ttaggtcact catagtattt
     3721 tgaagattga attcattaat tttaaaatgt ataatgatat ttgtgaaagc gcttgcttag
     3781 gaggtgtatt tgagagtgaa tgaaatgaat gctaaagaac aattagtgga caatttaatg
     3841 aaaacatcat cgcaattatt taaatttcac ggtgaagttg ccatgcagct tttcttaaat
     3901 gatgaattaa aattaccttc tattgttgaa atatgcgtgg aacgtaagcg tttaagtgat
     3961 attgtgaaag ttattccgca atcatatgcg ttactataca tagataagca agatcaagca
     4021 atagctaaag aagatttatc actttcaaaa attgcaaaag tttatgtgca atatgatgat
     4081 acaacaataa tgagtatttt cgtttatgat gtagtaaacg atgaatggat ttttagattg
     4141 gatccgaata tacgtatacc taagagtaac atatacttcc atagtttaaa ttgggatgtg
     4201 gattatatta aaccggagat tgttctaatg tatgatctaa tgcaacacca tcagtatcat
     4261 cattattcca attataaacg agtcatagat gcattaagct actatcaatt ttttatttta
     4321 aaatttgtag taggtgagca acgtattaag gatgcaatcc agagaacaat aaataattaa
     4381 gaaaaagcaa ttcataacgc agttgaatac atgtgttacg aattgctttt atattagttt
     4441 ttatcacaca agttttttaa tgcaaccccg tgatagcaaa actcatatgt agataataca
     4501 gctttttcag catcatctac atgaattcca aacatcatcg agatttccga agcaccttgg
     4561 ttaatcattt ttaagttaat ttttgattca gctaaggcat gtgtaatttt atttgcagta
     4621 ccgatgactt tattcatacc ttcgccgaca atcattaata ttgctaaatc atgctcaata
     4681 cttagctcat caacatcaca tttttgacga atttcattta atacttttgt ttctttattt
     4741 tgaatttgtt ttgaacgcat aacgatactg atagtatcaa tacctgaagg catatgatca
     4801 aatgaaatat tattatcctc taagacacct aatatctttc tagtaaaccc gacttgtcta
     4861 ttcattaaat actttttgat attaataacg gtaaaatctt tatcacaact tataccgcta
     4921 atcacatttt tcgcatttat ttctctatca tgcactataa atgtaccttt atcttgaggg
     4981 cggttcgtat ttttaatcac cacagggatg cgatctttat aaagtggttg taaggcttca
     5041 tcatggaaaa cactaaaacc agcataagat aattcacgca tttctcgata agtgatttct
     5101 tcgattaatt caggatcttt gatgatattt ggattagctt tataaatacc agacacatcg
     5161 gtgaaatttt cataaattgt agctctaaca ccacttgata tgatggcgcc agttatatct
     5221 gatccgccac gtggaaatgt aactatatat ccttcatgag atacgccgaa aaatcctggg
     5281 ataattagtt tctcatcata atctcttaat tttttaattt cagagtaagc actatctaat
     5341 atttgtgctt cttgtgggac gtcagtaaca aaaatacccg cttccttcgg tgatatatat
     5401 tttgttggta taccttgact attattatat aaagctatca attgcgcatt aaaatcttca
     5461 ccacaagaaa gtaatgcatc taatagtctc tttggttcat tttttaattg attaatataa
     5521 tgttccaaag tcacatctat cgtccgtaaa atactttcat ccatttgcaa ttcttttaca
     5581 atatcatcat aacgctgaat aatttctctt tttttatcat gataatcaag atgattaatg
     5641 accttttcat ataatctgat taacaaatca gttgttttaa tatcattatc atgtctttta
     5701 cctggagcag aaacgataac aatctttcgc tctggatcag aattaacaat atttaaaacc
     5761 tttttaattt gagtagcatt ggagacggag ctaccaccga atttggaaac tttcatagtg
     5821 catgccaacc tttctaaaaa atcagaaaat tgtattcagt tttgtaagta agaatgatat
     5881 tatgtattta tattttgaat attacgaact t
//
LOCUS       DAMZKC010000143         5871 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.145, whole genome shotgun sequence.
ACCESSION   DAMZKC010000143 DAMZKC010000000
VERSION     DAMZKC010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5871
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.145"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(40..888)
                     /gene="dat"
                     /locus_tag="POB55_002113"
     CDS             complement(40..888)
                     /gene="dat"
                     /locus_tag="POB55_002113"
                     /EC_number="2.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino-acid transaminase"
                     /protein_id="HDI6501068.1"
                     /translation="MEKIFLNGEFVSPSEAKVSYNDRGYVFGDGIYEYIRVYNGKLFT
                     VTEHYERFLRSANEIGLDLNYSVEELIELSRKLVDMNQIETGVIYIQATRGVAERNHS
                     FPTPEVEPAIVAYTKSYDRPYDHLENGVNGVTVEDIRWLRCDIKSLNLLGNVLAKEYA
                     VKYNAVEAIQHRGETVTEGSSSNAYAIKDGVIYTHPINNYILNGITRIVIKKIAEDYN
                     IPFKEETFTVDFLRNADEVIVSSTSAEVTPVIKLDGEPVNDGKVGPITRQLQEGFEKY
                     IESHSI"
     gene            complement(892..2301)
                     /gene="sapep"
                     /locus_tag="POB55_002114"
     CDS             complement(892..2301)
                     /gene="sapep"
                     /locus_tag="POB55_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mn(2+)-dependent dipeptidase Sapep"
                     /protein_id="HDI6501069.1"
                     /translation="MWKEKVQQYEDQIINDLKGLLAIESVRDDAKASEDAPVGPGPRK
                     ALDYMYEIAHRDGFTTHDVDHIAGRIEAGKGNDVLGILCHVDVVPAGDGWDSNPFEPV
                     VTEDAIIARGTLDDKGPTIAAYYAIKILEDMNVDWKKRIHMIIGTDEESDWKCTDRYF
                     KTEEMPTLGFAPDAEFPCIHGEKGITTFDLVQNKLAEDQDEPDYELITFKSGERYNMV
                     PDHAEARVLVKENMTDVIQDFEYFLEQNHLQGDSTVDSGILVLTVEGKAVHGMDPSIG
                     VNAGLYLLKFLASLNLDNNAQAFVAFSNRYLFDSDFGEKMGMKFHTDVMGDVTNNIGV
                     ITYDNENAGLFGINLRYPEGFEFEKAMDRFANEIQQYGFEVKLGKVQPPHYVDKNDPF
                     VQKLVTAYRNQTNDMTEPYTIGGGTYARNLDKGVAFGAMFSDSEDLMHQKNEYITKKQ
                     LFNATSIYLEAIYSLCVEE"
     gene            complement(2994..3416)
                     /locus_tag="POB55_002115"
     CDS             complement(2994..3416)
                     /locus_tag="POB55_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDI6501070.1"
                     /translation="MAKGNLFKAILGIGGAVAAVLVTRKDSRDKLKAEYNKYKQDPQS
                     YKDNAKDKATQLGTIANETIKEVKTNPKEYANRLKNNPKAFFEEEKSKFTEYDNKTDE
                     SIEKGKFDDEGGAAPNNNLRIVTEEDLKKNKNALSDKE"
     gene            complement(3433..4128)
                     /locus_tag="POB55_002116"
     CDS             complement(3433..4128)
                     /locus_tag="POB55_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDI6501071.1"
                     /translation="MRIDKFLANMGVGTRNEVKQLLKKGLVNVNEQVIKSPKTHIEPE
                     NDKITVRGELIEYIENVYIMLNKPKDYISATEDHHSKTVIDLIPEYQHLNIFTVGRLD
                     KDTEGLLLITNDGDFNHELMSPNKHVSKKYEVISANPITEDDIQAFKEGVTLSDGKVK
                     PAILTYIDNQTSHVTIYEGKYHQVKRMFHSIQNEVLHLRRIKIADLELDSNLDSGEYR
                     LLTENDFDKLNYK"
     gene            complement(4125..5786)
                     /locus_tag="POB55_002117"
     CDS             complement(4125..5786)
                     /locus_tag="POB55_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDI6501072.1"
                     /translation="MSESKEMVRGTFLITISILITKVLGVLFIIPFNYLIGGQENMAP
                     FTYAYAPYNIAIAVATAGVPLAASKYVAKYNAIGAYKVSQKFYKSSFIVMSITGVLGF
                     LVLYFLAPYISELTLARNVHDKNGWSVDDITWIIRIISMVVIFIPVLATWRGIFQGYK
                     SMGPTAVSEVTEQIARVIFILIGSYLVLNVFDGSILLANGIATFAAAVGAIIGIFTLW
                     YYWRKRKHNIDRMVESDYTDIDVSYGKMYKEIIAYSIPFVIVSLNYPLFNLVDQFTHN
                     GALSLVGIPSQLQDIFFNMLNMSTNKIVMIPTSLSAGFAVSLIPFITKTFAEGRLHEM
                     HHQIRTSIGVLMFITVPASIGIMALAQPLFTVFYGYDPIVLGHDPNHDGSRLLFYYAP
                     VAILISLLSVTASMLQGIDKQKLTVYVILASVVIKLALNYPLIMLFHTPGAILSTSIA
                     LLFAIGCNFYILKKYAKFKFSYSWIHFAKIFLYSFIMMLGVELVFFLANLFLEPTKLG
                     YLIIIILGVTVGILIYGTITIKTRLADEFLGEIPEKLRRRVRFLR"
BASE COUNT         1909 a         1102 c          807 g         2053 t
ORIGIN      
        1 aatttaagta atttaatcta aaaatatgat gaaagaattt taaatactgt gtgactctat
       61 atacttttca aatccttctt gtagttgacg tgtaattggg ccaactttac catcattaac
      121 tggttcacca tctaatttaa taacaggtgt aacctcagct gaagtacttg aaacaataac
      181 ttcatctgcg tttctcaaga aatctacagt aaacgtttct tctttaaatg ggatattata
      241 gtcttcggca atttttttaa ttacaattcg tgtaatacca ttaagaatat agttgttaat
      301 cggatgtgta taaatcacac cgtctttaat tgcataagca ttacttgaag atccttcagt
      361 gacagtttcg cctcgatgtt ggattgcttc aacggcattg tattttacag catattcttt
      421 tgctaataca tttcctaata aattcaagct tttaatgtcg caacgtaacc atcggatatc
      481 ttcaacggta acaccattca caccattttc taaatgatca taaggacgat cataactctt
      541 tgtataagca acaattgctg gttctacttc aggtgttggg aagctatgat ttctttcagc
      601 tacacctcgc gttgcttgaa tataaattac cccagtttca atttgattca tatcaactaa
      661 tttacgagat agttcaatta attcttctac agaataattt aaatctaaac caatctcatt
      721 ggcactacgt aaaaatcttt cataatgttc tgttactgta aataacttac cattatatac
      781 tcgaatgtat tcataaatac catcgccaaa tacgtatcct ctgtcgttgt atgaaacctt
      841 tgcttcactt ggacttacaa actcaccatt taaaaaaatt ttttccatat attattcctc
      901 cacgcataat gaataaattg cttctaagta aatactagtt gcgttaaata actgtttttt
      961 agtgatatat tcatttttct gatgcattaa atcttcagaa tcactaaaca ttgcgccaaa
     1021 tgctacaccc ttgtctaagt ttctcgcata agtaccgcca cctatagtat aaggttcagt
     1081 catatcattt gtttgatttc tatatgcagt aactaacttt tgtacaaaag gatcattttt
     1141 atcaacataa tgtggtggtt ggactttacc taatttcact tcaaagccat attgttgaat
     1201 ctcatttgca aaacgatcca tagctttttc aaattcaaat ccttctggat agcgcaagtt
     1261 gataccgaaa agacccgcat tttcattatc atatgtaata acaccaatgt tatttgtcac
     1321 gtcacccatg acatctgtat ggaatttcat tcccatcttt tcaccaaaat ctgaatcaaa
     1381 taagtagcga ttactaaatg ctacaaacgc ttgtgcatta ttatcaagat ttaatgatgc
     1441 caagaatttt agtaagtaaa gacccgcatt cacaccgata gatggatcca taccatgaac
     1501 cgctttacct tcaactgtta aaactagaat gccactatca acagtactat caccttgtaa
     1561 atgattttgt tctaaaaagt actcaaagtc ttgaataaca tctgtcatat tttctttaac
     1621 aagcactctt gcttctgcat gatcaggcac catgttgtaa cgttcaccag atttaaaagt
     1681 tattaattca taatcaggtt catcttgatc ttcagcaagc ttattttgaa ctaagtcaaa
     1741 tgttgtaatg cctttttcac catgaataca cggaaattct gcatctggtg caaaacctaa
     1801 tgttggcatt tcttctgttt taaaatagcg atccgtacat ttccaatcag attcttcatc
     1861 cgtaccaata atcatatgaa tacgtttctt ccaatccaca ttcatatctt ctaatatctt
     1921 aattgcataa taagcagcaa ttgttggacc tttgtcatca agtgtacctc tagctatgat
     1981 agcatcttct gttacaaccg gctcgaacgg attactatcc catccatcac cagcaggaac
     2041 aacgtcaaca tgacataaga tacctaatac gtcatttcct ttacctgcct caattcttcc
     2101 tgcaatatga tccacatcat gtgttgtaaa tccatctcta tgtgcaattt catacatgta
     2161 gtctaatgcc ttacgaggac ctggaccaac tggtgcgtct tctgatgctt ttgcatcatc
     2221 tctcacactt tcaattgcta ataatccttt taagtcatta atgatttgat cttcgtattg
     2281 ttgaactttt tctttccaca ttcgaaatcg acttcctttt ttctataagt taaattctat
     2341 tttacatgaa atgatataaa aactacaata agatgtcaga aaataataaa aaggaacaaa
     2401 acgatgctat cgatatgaca caaatcataa atagctgctt tgttcctttt ttaatttata
     2461 tatttaaaat acacatattc aagaactcga gatataagtc aatgtactag gcacacaatt
     2521 taatattgac agtaattaac cgaacgaaaa tgcgccccgg ggcccccaac atagagaatt
     2581 tcgaaaagaa attctacaga caatgcaagt tggcgaggcc ccaacacaga aactggccaa
     2641 cagtcagctt tcaataatgt gcaagttggg gtaagggccc caacatagag aatttcgaaa
     2701 agataaagaa atacgttttc tttagatatt agtatttttt atgaatgagt ttcacgcatg
     2761 tattcttctt tctatatgca tattagctat gactaacgat aaagaacctg aaacactaat
     2821 aaatgtccta tagtttacaa tattatattg gcagtagttg actgaatgaa aatacgcttg
     2881 taacaagctt tttttcaatt ctagtcaacc ttgccggggt gggacgacga aataaatttt
     2941 gctaaaatat gatttctgtc ccactccctt atcatttctg tcctactcac atcttattct
     3001 ttatcagata gtgcattttt attctttttt aagtcttctt cagtgacgat acgtaaatta
     3061 ttatttggtg cggcgccacc ttcatcatca aatttacctt tttcaatact ttcgtcagtc
     3121 ttattgtcat attcggtaaa ttttgatttt tcttcttcga aaaatgcttt tggattattt
     3181 tttaatctat tagcatattc tttcggattt gtttttactt ctttaattgt ttcattagca
     3241 attgttccta attgcgtcgc tttatcctta gcattatctt tatagctttg aggatcttgt
     3301 ttatatttat tatattctgc tttcagcttg tcacgactat ctttacgtgt aacaagtaca
     3361 gctgctacag cgccacctat acccaaaatc gctttaaata aattaccttt tgccatatca
     3421 atcgtctccc ttttatttat aatttaattt gtcaaaatca ttttcagtta ataaacgata
     3481 ttctcctgaa tctagattgc tgtccaattc taaatcagca attttgatac gtcttaaatg
     3541 taatacctca ttttgaatgc tatgaaacat tcgtttaact tgatgatatt ttccttcata
     3601 aattgttacg tgtgacgttt gattatcaat ataagttaaa attgcaggct taaccttgcc
     3661 atcagacagt gttacaccct ctttaaaagc ttgaatgtcg tcttcagtga taggatttgc
     3721 tgaaataact tcatattttt tagaaacatg tttgtttgga ctcattaatt catgattaaa
     3781 atcaccatca ttcgttatca ataaaagccc ttctgtatct ttatcaagac gaccaaccgt
     3841 aaaaatattt agatgttggt attcaggtat taaatcaata acggtttttg aatgatgatc
     3901 ttcagttgct gatatataat cttttggctt atttaacata atatagacat tttcaatgta
     3961 ttctattaat tctccacgaa ctgttatctt atcgttttct ggttctatat gtgtttttgg
     4021 tgatttaatt acttgttcgt tgacatttac aaggcctttt ttaagtaact gtttgacctc
     4081 attacgtgta ccgacgccca tatttgctaa aaatttatct attctcatcg taaaaaccta
     4141 actctacgtc ttaatttttc aggaatttca cctaagaatt cgtccgcaag acgcgtttta
     4201 attgtgattg taccgtaaat tagaatacct actgtaacac ctaaaataat aatgattaag
     4261 taaccaagtt tagtaggttc taagaataga tttgcaagga agaatactaa ttctacacct
     4321 agcatcataa taaatgaata caagaatatt tttgcaaaat gaatccaact atagctgaat
     4381 ttaaacttcg catatttttt aagaatatag aaattacatc caattgcaaa taataatgcg
     4441 atactagtac ttaaaattgc accaggtgta tggaataaca taattaatgg atagtttaac
     4501 gctaacttga taactacaga agctaaaata acataaactg ttaatttctg tttatctata
     4561 ccttgtaaca ttgatgccgt tacacttaat agtgaaatta gtattgctac aggcgcataa
     4621 tagaataata agcgactacc atcatggtta gggtcatgac ctaaaacaat tggatcgtaa
     4681 ccatagaaaa ctgtgaataa tggttgtgcc aaggccataa ttccaatact agctggaaca
     4741 gttataaaca ttaatacacc aatagatgtt ctaatttgat gatgcatttc atgtaagcga
     4801 ccttctgcaa atgtttttgt aataaaagga attaaactca ctgcaaaacc agcacttaat
     4861 gatgtcggaa tcattacaat tttattagtt gacatattta gcatattaaa gaatatatct
     4921 tgtaactgtg aaggtatacc aactaaagat aaagcaccgt tatgtgtaaa ttgatctact
     4981 aagttaaata atggataatt caaacttaca ataacgaacg gaatactata agcaataatt
     5041 tctttataca tcttgccata tgacacatct atatctgtgt aatcagattc gaccatacga
     5101 tcaatattat gcttacgctt tctccagtaa taccagagtg tgaatatacc aataatcgca
     5161 ccaactgctg ctgcaaaagt agcaatacca ttggctaata aaatagagcc atcaaagaca
     5221 tttagtacta aataacttcc gattaatatg aaaatcacgc gtgcaatttg ctcagttact
     5281 tctgacactg ctgttggccc catagattta taaccttgga atatacctct ccatgtcgct
     5341 aatacaggaa taaagataac aaccatacta atgattctta taatccaagt aatatcatcg
     5401 actgaccaac cgtttttatc atgaacgttt ctagctaatg ttaattcaga aatataaggt
     5461 gctaggaaat acagtaccaa gaaacctaaa acaccggtaa tactcattac aataaaactc
     5521 gatttataaa atttctgact gactttatat gccccaatag cattatattt cgcaacatat
     5581 ttcgaagctg ctaatggtac acctgctgtc gcaactgcaa ttgcaatatt atatggtgca
     5641 taagcgtatg tgaacggcgc catattttct tgtccaccaa ttaaatagtt gaatggaatg
     5701 ataaaaagta cgcccaatac cttggtaatt aatatactaa tggtaattaa aaaggttcca
     5761 cgcaccattt ctttactttc actcattacg aatctcccta tctcatgttt attaaagttt
     5821 tgtaaactaa aagctatttc tctgtaaaat catttttcat tattatgaat a
//
LOCUS       DAMZKC010000144         5842 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.146, whole genome shotgun sequence.
ACCESSION   DAMZKC010000144 DAMZKC010000000
VERSION     DAMZKC010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5842
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.146"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            29..1105
                     /locus_tag="POB55_002118"
     CDS             29..1105
                     /locus_tag="POB55_002118"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HDI6501073.1"
                     /translation="MSQAVKVERRETLKQKPNTSQLGFGKYFTDYMLSYDYDADKGWH
                     DLKIVPYGPIEISPAAQGVHYGQSVFEGLKAYKRDGEVALFRPEENFKRLNNSLARLE
                     MPQVDEAELLEGLKQLVDIERDWIPEGEGQSLYIRPFVFATEGALGVGASHQYKLLII
                     LSPSGAYYGGETLKPTKIYVEDEYVRAVRGGVGFAKVAGNYAASLLAQTNANKLGYDQ
                     VLWLDGVEQKYIEEVGSMNIFFVENGKVITPELNGSILPGITRKSIIELAKNLGYEVE
                     ERRVSIDELFESYDKGELTEVFGSGTAAVISPVGTLRYEDREIVINNNETGEITQKLY
                     DVYTGIQNGTLEDKNGWRVVVPKY"
     gene            1356..2039
                     /locus_tag="POB55_002119"
     CDS             1356..2039
                     /locus_tag="POB55_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDI6501074.1"
                     /translation="MEWILFDKDGTLIEFDRSWEKIGVRFVQSLLETFPVHNKEAALR
                     QLGVIKESIDPKSVMGSGSLQQIIQAFNDVTGQDTTDWSKSTSQKLVDERIPEINWVE
                     GVKEALIDLKAKGYQLGIVTSDTKKGVEQFLAHTNATSLFDLIISTEADAYEKPNPKV
                     LSPLFEQYNVDPQKVAIVGDTANDMKTASNANLGMAIGVLTGIATKEELHEADIILNS
                     AADILEALN"
     gene            complement(2155..2817)
                     /locus_tag="POB55_002120"
     CDS             complement(2155..2817)
                     /locus_tag="POB55_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDI6501075.1"
                     /translation="MNNYGIPQNAIITIAGTVGVGKSTLTQALADKLNFKTSFENVEH
                     NPYLDKFYSDFERWSFHLQIYFLAERFKEQKRMFEYGGGFVQDRSIYEDVDIFAKMHE
                     EEGTMSKEDFKTYSDLFNAMVMTPYFPKPDVMIYLECNYDEVIDRIIERGREMEINTD
                     PEYWKKLFKRYDDWINSFNACPVVRININEYDIHKDPESLNPMIDKIARIIQTYRQVD
                     TR"
     gene            complement(2810..3427)
                     /locus_tag="POB55_002121"
     CDS             complement(2810..3427)
                     /locus_tag="POB55_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDI6501076.1"
                     /translation="MNKPFIAIEGPIGVGKSSLAHKLSQTLDFYEEKEIITENPFLSD
                     FYEDISKWSFQTEMFFLCNRYKQFQDVTQLNQGVVSDYHIHKNKIFAKNTLSSVEFQK
                     FSKIYDILTEDMIMPNMIIFLDADLDVLKSRIAKRNRSFEHQIEDEYLLKLKKDYREY
                     YESLQSNGSNVVLIDTTSIDFLKNEQDYEDILHIILPMIGDITNE"
     gene            3494..3964
                     /locus_tag="POB55_002122"
     CDS             3494..3964
                     /locus_tag="POB55_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HDI6501077.1"
                     /translation="MTNDIYFMTLAIEEAKKAAQLGEVPIGAIITKDDEVIARAHNLR
                     ETLQQPTAHAEHIAIERAAKVLGSWRLEGCTLYVTLEPCVMCAGTIVMSRIPRVVYGA
                     DDPKGGCSGSLMNLLQQSNFNHRAIVDKGVLKEACSTLLTTFFKNLRANKKSTN"
     gene            4111..4980
                     /locus_tag="POB55_002123"
     CDS             4111..4980
                     /locus_tag="POB55_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDI6501078.1"
                     /translation="MIKLIATDMDGTLLNAAHEISQPNIDAIKYAQEQGITVVIATGR
                     AFYEAQAPVADTDLTVPYICLNGAEVRDETFNVMSTSHLNKSLVHKITNVLKDAGIYY
                     QVYTSRAIYTEDPQRDLDIYIDIAERAGQHANVERIKNGIQRRIDNGTLKVVDNYDAI
                     ENIPGELIMKILAFDGNLEKIDKASKILAESPNLAISSSSRGNIEITHSDAQKGIALE
                     TIAERLGIEMKEVMAIGDNLNDLSMLEKVGYPVAMENGAEEVKKIAKYVTDTNENSGV
                     GKAIMKLLREQQV"
     gene            5001..5567
                     /locus_tag="POB55_002124"
     CDS             5001..5567
                     /locus_tag="POB55_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDI6501079.1"
                     /translation="MKGLIIIGSAQVNSHTSALARYLTEHFKTHDIEAEIFDLAEKPL
                     NQLDFSGTTPSIDEIKQNMKDLKEKAMAADFLILGTPNYHGSYSGILKNALDHLNMDY
                     FKMKPVGLIGNSGGIVSSEPLSHLRVIVRSLLGIAVPTQIATHDSDFAKNEDGSYYLN
                     DSEFQLRARLFVDQIVSFVNNSPYEHLK"
BASE COUNT         2139 a          802 c         1049 g         1852 t
ORIGIN      
        1 tatacaatat tattaggagg caatcaccat gtcacaagca gttaaagttg aacgacgaga
       61 aacattaaaa caaaaaccaa atacatctca actaggtttt ggtaaatatt ttactgatta
      121 tatgttgagt tatgattatg atgcagataa aggatggcat gatttgaaga tagtacctta
      181 tggtcctatt gaaatttcac ctgctgcaca aggtgttcat tatggtcaat cggtattcga
      241 aggattaaaa gcatataaaa gagatgggga agttgcactt ttccgtcctg aagaaaattt
      301 taagcgtctt aataactcgt tagcacgatt agaaatgcct caagtagacg aagcagaatt
      361 gttagagggg ctaaaacaat tagttgatat agaaagagat tggattcctg aaggggaagg
      421 tcaatcatta tatattcgtc catttgtttt tgcaacagaa ggggcacttg gcgttggtgc
      481 atcacatcag tataaattat taattatttt atctccttca ggtgcatatt atggtggtga
      541 gactttaaaa ccaactaaaa tctatgtaga agatgaatat gtgcgtgctg ttcgtggcgg
      601 tgtaggcttt gcaaaagttg caggtaacta tgcggcaagt ttattagcac aaacaaatgc
      661 aaataaatta ggttatgacc aagtattatg gcttgatggt gttgaacaga aatatatcga
      721 agaagttggt agcatgaata ttttcttcgt tgaaaatgga aaagtaatta caccagagtt
      781 gaatggcagt attttacctg gtattacacg taaatctatt atcgaattag ctaaaaactt
      841 aggatatgaa gtcgaagagc gccgcgtttc aatcgatgaa ttattcgaat catatgataa
      901 aggtgagtta acagaagtat ttggtagtgg tactgcagca gttatttcac ctgtgggtac
      961 attgagatac gaagatcgtg aaatcgttat taataataat gagactggtg aaattactca
     1021 aaaattatac gacgtctata ctggtattca aaatggtact ttagaagata aaaatggttg
     1081 gagagtcgtt gtaccaaaat attaataaaa attgaatata atcatgaaaa caatatgtaa
     1141 aagttaatca tgactaaaat ccctttcaat gaagcgaagc atggtaataa attaagttta
     1201 caatgtttat tgttaagtat tgaaggggat ttcacttatt attatattta attcaatatt
     1261 taaaaagaac aatactacga gttcattttc agggaaataa cgaacatcat agaatcagca
     1321 taatacgaaa taaataaata ggagtattga tattaatgga atggatatta tttgataaag
     1381 atggcacgtt aattgaattt gatagaagtt gggaaaaaat aggggtacga tttgtacaat
     1441 cattgcttga gactttccca gtacataata aagaagctgc tttaagacaa ctcggtgtca
     1501 ttaaagaatc tattgatcca aaatcagtga tgggttcagg atctttacaa caaattatcc
     1561 aggcatttaa tgatgtgacg ggacaagata caaccgactg gtccaagtca acaagtcaaa
     1621 agctggtaga tgaacgtatt cctgaaatta attgggtaga aggtgttaaa gaagcactta
     1681 tcgatttgaa agcaaaaggc tatcaacttg gtattgttac gagtgatact aaaaaaggtg
     1741 tagaacaatt tttagcacat accaatgcta cctcgttgtt cgatttgatc atttctaccg
     1801 aagcggatgc ctatgagaag ccaaatccta aagtattatc gcctttattt gagcaatata
     1861 atgtagatcc tcagaaagta gctatagtag gagacactgc taatgatatg aagacagcaa
     1921 gtaatgcaaa tttaggtatg gcaataggtg tattaacagg tattgcaaca aaagaagaat
     1981 tacatgaagc tgatattatt ttaaatagtg cggcagatat tttagaagct ttaaattaaa
     2041 aagaaagaca tagtgtatat gtgaattaat gtattcgata gaattgataa tgtaatcgat
     2101 gatatctatg gaatatacat tgaatataaa catatacgct atgtctttag tcttttatcg
     2161 tgtatctact tgtcgatatg tttgaataat tcgagcaatt ttgtctatca taggatttaa
     2221 agattcgggg tccttatgga tatcatattc attaatattg atacgtacaa ctggacatgc
     2281 attaaagcta ttaatccaat cgtcatagcg tttaaatagc tttttccagt attcagggtc
     2341 tgtattaatt tccatttcgc gaccacgttc aataatacga tcaatgacct catcatagtt
     2401 acattctaaa taaatcatta catcaggttt aggaaaataa ggtgtcatga ccatggcatt
     2461 aaataagtct gaatatgttt tgaaatcttc tttactcatt gtgccttctt cttcatgcat
     2521 ttttgcaaaa atatcaacat cttcataaat tgatcgatct tggacaaagc caccaccata
     2581 ttcaaacata cgcttttgtt ctttaaaacg ttcagctaag aagtaaattt gcaaatggaa
     2641 actccatcgt tcaaaatcgc tgtaaaattt atctaaatat ggattatgtt cgacattttc
     2701 aaaagacgtt ttaaagttta atttatctgc aagtgcttgc gttagtgttg attttccaac
     2761 accaactgta cctgcaatgg ttataatggc attttgtgga ataccgtaat tattcattgg
     2821 taatatctcc tatcataggt aatataatat gtaatatatc ttcgtaatct tgttcatttt
     2881 taagaaaatc aatagaagtt gtatcgatta aaactacatt tgaaccatta ctttgtaagg
     2941 actcataata ctcacgataa tcttttttta actttaacag atattcatct tctatttgat
     3001 gctcaaaact acggttacgt ttagcaattc tagattttaa cacatcaagg tctgcatcta
     3061 aaaagataat catattcggc ataatcatat cttcagttaa aatatcataa attttactga
     3121 atttctgaaa ttcaacagaa ctcaaagtat ttttagcaaa tatcttattt ttatgtatat
     3181 gataatcact aactacacct tgatttagtt gtgttacatc ttgaaattgc ttatatctat
     3241 tgcataaaaa gaacatttca gtttgaaaac tccatttaga gatatcttca taaaagtctg
     3301 ataaaaatgg attttctgtg atgatttctt tttcttcata aaaatctaaa gtttgactta
     3361 atttgtgtgc aagtgaagat ttacctacgc caataggacc ttcaattgct ataaaaggtt
     3421 tgttcatatt cacatctcca aatgttaaaa tagacattga gtattgtacc ataagtgagg
     3481 tatctggaca taaatgacaa atgatatata ttttatgaca ttagcgattg aagaagctaa
     3541 aaaagcagct caactaggcg aagtacctat aggtgctatc atcactaaag atgatgaagt
     3601 tatcgctaga gcacataatt taagagaaac actacaacaa ccaacggcgc atgctgaaca
     3661 tattgcaatt gaacgtgcag ccaaagtgtt aggtagttgg cgtttagaag gttgcacatt
     3721 atatgtaacc ttagaaccat gtgtcatgtg cgcaggaaca attgtaatga gtcgcattcc
     3781 aagagtcgtc tatggcgcag atgatcctaa aggtggttgt agtggcagtt taatgaattt
     3841 attgcaacaa tctaatttta atcatcgtgc aattgttgat aaaggtgtac ttaaagaagc
     3901 atgtagcaca ttattaacaa cattttttaa aaacttaaga gccaataaga aatccaccaa
     3961 ttagacgata gtacatacta attaaaattt gttagaatta cattatatga taaattaatg
     4021 acaatataaa tgttaataga aaagtatgtg cgctgagtat atattcttat ttaagaacaa
     4081 gattttaaat ttacgaaacg aggtaacata atgataaaac taatagccac tgatatggat
     4141 ggcacgctac ttaatgcagc acatgaaatt tctcaaccta atattgatgc gattaaatac
     4201 gctcaagaac aagggataac ggttgttatc gcgacaggtc gagcatttta tgaagcacaa
     4261 gcaccagttg ctgacacaga tttaacagta ccatatattt gtttgaatgg tgctgaagta
     4321 cgtgatgaaa ctttcaatgt aatgagcact tcacacctta ataaatcgct agtacacaaa
     4381 attacaaatg ttttaaaaga tgcaggtatt tattatcaag tatacacgag tcgtgcgatt
     4441 tatactgaag atccacaaag agatttagac atttacatag atattgctga gcgtgcaggt
     4501 caacatgcaa acgttgagcg tattaaaaat ggtattcaaa gacgcataga taatggtacg
     4561 ttgaaagttg ttgataatta tgatgctatt gaaaacatac ctggtgaatt aattatgaaa
     4621 atattagcat ttgatggaaa tttagaaaaa attgacaaag ctagtaaaat tttagctgaa
     4681 tctccgaatt tagctatatc atcatcttcg agaggaaata tagaaataac gcattcagat
     4741 gcacaaaaag gtattgcgct agaaacaatt gccgaaagat tagggattga aatgaaagaa
     4801 gtcatggcaa taggtgacaa tttaaatgac ttatcaatgt tagagaaagt tggctatcca
     4861 gttgcgatgg aaaatggtgc agaagaagtt aaaaaaatag cgaaatatgt cacagatacg
     4921 aatgaaaata gtggtgttgg aaaagctatt atgaaattat tacgtgaaca acaagtttaa
     4981 taaaaaaaga ggggtcaaat atgaaaggat taattattat tggcagtgca caagtgaatt
     5041 cacatacaag tgcactagca agatacttaa ctgagcattt taaaacacat gatattgaag
     5101 cggaaatatt cgatttagca gaaaaaccgt taaatcaatt agatttttca ggaacaacac
     5161 cgtctattga tgaaatcaaa caaaatatga aagatttaaa agagaaagca atggcggcgg
     5221 actttttaat attaggaacg ccaaactatc atggttcata ttctggaata ttgaaaaatg
     5281 cattagatca tctaaatatg gattatttta aaatgaaacc tgtaggctta ataggaaata
     5341 gtggtggtat tgttagttca gagccattgt cacatttaag agtaatcgtc agaagtttac
     5401 taggcattgc tgtaccaact caaatagcaa cacatgattc tgattttgct aaaaatgaag
     5461 atggttcata ttacttaaat gatagtgaat tccaattacg agcaagatta tttgtcgatc
     5521 aaattgtatc ttttgtgaat aatagtccat atgaacattt aaaataatat taaaaaatat
     5581 gtaaatatac attaaaaata ggtatgtagc ttaaggtatt tcattgagaa tgcttttaag
     5641 tcacatgcct attttttgtt ctaatgcacc aatagtaagc ctttgatatt actgttgtga
     5701 gcaatgttat taattaaaat aagatgtaat agcgaattga aaacaagctt agattaaaag
     5761 tgagttttaa aaatatagat aaataattta aagcaattaa agaaaaaaag tattaaaaat
     5821 tgcaattctt aaactgtgat tc
//
LOCUS       DAMZKC010000145         5835 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.147, whole genome shotgun sequence.
ACCESSION   DAMZKC010000145 DAMZKC010000000
VERSION     DAMZKC010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5835
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.147"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(<1..847)
                     /locus_tag="POB55_002125"
     CDS             complement(<1..847)
                     /locus_tag="POB55_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease"
                     /protein_id="HDI6501080.1"
                     /translation="MPNKILLVDGMALLFRHFYATSLHKQFMYNSQGVPTNGIQGFVR
                     HIFSAIHEIRPTHVAVCWDMGQSTFRNDMFDGYKQNRSAPPEELIPQFDYVKEISEQF
                     GFVNIGVKNYEADDVIGTLAQQYSTDNDVYIITGDKDLLQCINDNVEVWLIKKGFNIY
                     NRYTLHRFNEEYALEPQQLIDIKAFMGDTADGYAGVKGIGEKTAIKLIQQYQSVENVV
                     ENIDALSAGQRNKINDNLDELYLSKRLAEIHTQVPIDSESLFEKMSFATTLNHILSIC
                     NEHELH"
     gene            complement(867..4307)
                     /locus_tag="POB55_002126"
     CDS             complement(867..4307)
                     /locus_tag="POB55_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HDI6501081.1"
                     /translation="MINKEQLDLLYKLKKEVEKSRNEALLHTINQVIKKVYLQQYTCS
                     FVGHFSAGKSTMINLLIEQDILPSSPVPTTSNTAIVSVSDNHDIIANLPNQTYAKLSN
                     YDEVREMNRQNVDVESVEINFQSAKFENGFTLQDTPGVDSNVASHQSITEQYMYTSNM
                     IFYTVDYNHVQSELNFKFMKHINDVGIPVVFIINQIDKHQDDELSFSTFKSRVEKSIA
                     DWGIKLERTFYVSKFDHPENELEVLSSYLISLDQHRETIEDYTSRTVEYITEAQLDYI
                     QSEIQEVLEDLGIEEAEFEQAFLNSQQHQAISEEAQLLNNPDELMAFLKNKRKNILEN
                     AYIMPHNMREMLRSYLESMSQDFNVGGFFNKKKKKLQIQQQRLLTATDALQEHVNQQI
                     RQPMREDMSFVTRFINKKEASDKVLNQHYDVKPEMIEDLYQPQTSISNTYVLTFSDEV
                     VKAIKKYVEQQSTPIFKEIIENVQADELPTEESDDLKEYQRYTELNELRQSLTTKNYR
                     HYYIHLDESLDKLIGRQETTYQVATDNTQDNRDNQQLNQNTATTNMSIDIQKALDIIS
                     DVPLFKRTKQDIHETLTRIDNKLIKIGVFGTFSAGKSSLINALLGEHILVSSPNPTTA
                     ATTEISYGDESYITLKSQSQLLDEINAVVEYQDMSYSTIEDFINSDLEKLKSHLNKNQ
                     LAFIQAVEKHYKLYVNMLENGEKHAINQQELKKWSAEDEYATFVKTVHIALMHDWLKG
                     KIIVDSLGLHSNNQRHTNETEQILTSSDLILYVSYFNHSFTDNDKAFIEHMKDMNQLN
                     ENQAFKMVINAADLAESQDDLEAVETYVSDALRQVHLQSDIFAVSSRNALQAEDKGID
                     QLKQSIQQFVDVESKSILEQQMIHQLKQMDRSYVEMITEFETNKADISRRQQRLTVYK
                     DKQRLQHQLIDATLQHTDNEVEEQVYHLNARLKLQLLDDVKSVFNSQMTQNSDFNEEK
                     KVSTKVYLDQIHQRLFLEQSLITERIKKYFNKQLTEQIAPIVQQLADLHVIVNPQFNF
                     ESANIEQPLLHIDFNDMLNALPKQLTKRKILNPNGQRDIHESICQSTLGLLQPQMGLL
                     RQQLELYVKQMAVEAESQFESFEANIQTQINDLLAFDLDTTLINQLKDKHQQLKTILY
                     "
     gene            4610..>5835
                     /locus_tag="POB55_002127"
     CDS             4610..>5835
                     /locus_tag="POB55_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDI6501082.1"
                     /translation="MKNTFNPLQRLHFYAAIFIAPLLITLTISGIGYLFFPEVENNIY
                     KNEFFGDSDVKTHQTLNDAVHQVEQQYEGFFVSKVSILDEPYNKRITLSDMAGNQRYV
                     FLDHNNQFVADQNAKHTYSNVMRSIHSSLFTENTIINYLVELTACWMIFMILSGTYLL
                     IKKHLISNKSKALRWQKWHAMIGVIIAIPVFVLVLTGLPWSGFMGSKIAGMMDTNGDL
                     GQGELAINPPKSDLNELPWATRKNKQPASSEKGSSGHHGNAAMPQTKLDYQISIDKVV
                     EQAQKAGIKKPFSIVYPSDKNGTFIVSNTSNSGVTGLDVSPYKEQTLYFDQYSGKKLG
                     TIKYDDYGIIAKWFTWGIPLHEGHLFGILNKIINLFVCIALLVAIGMGFVSWIKRTKN
                     TAVKVPHRVKKPASISL"
BASE COUNT         1816 a          955 c          878 g         2186 t
ORIGIN      
        1 catgtaattc atgttcgtta caaattgata aaatatgatt aagtgttgtt gcaaatgaca
       61 ttttctcaaa aagcgattca ctatcaattg gtacttgtgt gtgaatttca gcaagtcgct
      121 tcgataaata tagttcatct aaattgtcat taatcttatt acgttgccca gctgataatg
      181 catcaatgtt ttcaaccaca ttttcaacgc tttgatattg ctgaattaac ttaatagccg
      241 ttttctcacc aattccttta acacctgcat atccatctgc tgtatcaccc ataaacgctt
      301 taatatcgat tagttgttgt ggttcaaggg catattcttc gttaaaacga tgtaatgtat
      361 atctattata aatgttaaaa ccttttttaa ttagccagac ttcaacattg tcattaatac
      421 attgcagtaa atctttgtcg cccgtaataa tatagacatc gttatcagtt gaatattgtt
      481 gtgctaatgt acctataaca tcatccgctt catagttttt aacgccaata tttacaaagc
      541 caaattgctc tgaaatttct ttaacataat caaattgtgg tatcaattct tctggtggtg
      601 cagaacgatt ttgcttataa ccatcaaaca tatcatttct aaaagttgat tgtcccatat
      661 cccaacatac agctacatgt gtagggcgta tttcatgtat tgccgaaaag atatgacgca
      721 caaacccttg tattccattt gtaggtacac cttgtgaatt gtacataaat tgtttatgaa
      781 gacttgtagc gtagaaatgt ctaaataata gtgccatacc atctacaagt aatattttat
      841 taggcattta aaacgttcct tctttcttaa tataaaatag ttttcagttg ttgatgttta
      901 tctttcaatt gattgataag tgttgtatct aaatcaaatg ctaataaatc gtttatttgc
      961 gtttgaatat tagcttcaaa actttcaaat tgcgattcag cttctacagc catttgctta
     1021 acatataatt caagttgttg cctcaataat cccatttgtg gttgtaataa tcctaatgta
     1081 ctttgacaaa ttgattcatg tatatctctt tgcccatttg gattcaaaat tttacgtttt
     1141 gttaattgtt taggcaatgc atttagcata tcattgaaat cgatgtgcaa taatggttgc
     1201 tctatattag ctgattcaaa gttaaactga ggattaacaa tgacatgtaa atctgctaat
     1261 tgttgaacga ttggtgcaat ttgctcagtg agttgcttat taaagtattt ttttatacgt
     1321 tctgtaatta aagattgctc taaaaacaat cgttgatgaa tttgatctaa gtatactttc
     1381 gtagacactt tcttttcttc attaaaatca ctattttgcg tcatttgaga attaaacact
     1441 gatttaacat cgtcaagtag ttgtaatttt aaacgtgcat ttaaatgata aacttgttct
     1501 tcaacttcgt tgtctgtatg ttgtaacgtc gcatcaatta attgatgttg taaacgctgt
     1561 ttatctttat agactgttaa cctttgttgg cgacgtgaaa tatcagcttt atttgtttca
     1621 aattctgtaa tcatctctac ataagaacga tccatttgtt taagctgatg aatcatttgt
     1681 tgttctaaaa ttgatttaga ttcaacatca acaaattgtt gtatgctttg ttttaattga
     1741 tcaatgccct tatcttcagc ttgtaatgca tttcgacttg atacagcaaa aatgtctgat
     1801 tgtaagtgta cttgtcttaa tgcatctgat acatatgttt caaccgcttc aagatcatct
     1861 tgactttctg ctaaatcagc agcattaatt accattttaa atgcttggtt ttcattcaac
     1921 tggttcatat ctttcatgtg ttctataaac gccttgtcat tatcagtaaa tgaatgatta
     1981 aaataactta catacaatat taagtctgaa gaagttaaaa tttgctcggt ttcatttgta
     2041 tgcctttggt tatttgagtg tagccctaat gaatcaacaa ttattttacc ttttaaccaa
     2101 tcatgcatta atgcaatgtg tactgtctta acaaatgttg catattcatc ttctgcactc
     2161 cactttttca attcctgttg attaatggca tgtttttctc cattttccaa catattgaca
     2221 tacaatttat aatgtttttc aactgcctga ataaaagcga gttgattttt atttaaatgt
     2281 gattttaact tttctaaatc tgaattaata aagtcttcta tagtcgaata agacatatct
     2341 tggtattcaa ctactgcatt aatttcatct aataattgcg attgtgattt tagcgttata
     2401 taactctcgt ctccataaga tatttctgta gtagctgctg tagtaggatt tggagaactg
     2461 actaaaatat gctcgcctaa taatgcattt atcaaactac ttttaccagc actaaatgtt
     2521 ccaaatacac ctatttttat taatttatta tctatacgtg ttaatgtttc gtggatatct
     2581 tgctttgtac gcttgaacaa aggcacatcc gaaattatat caagcgcttt ttgaatatct
     2641 atagacatat ttgttgtagc tgtattttga tttagctgct gattatcacg attatcctga
     2701 gtattatcag tagccacttg atatgttgtc tcttgtcgac ctattaattt atctagagat
     2761 tcatctaaat gaatatagta gtgacgataa ttcttagtcg tcaatgactg acgcagctca
     2821 tttaattctg tataacgttg atattctttt aaatcatcac tttcttctgt tggtaattca
     2881 tctgcctgta cattttctat tatttcttta aaaattggtg ttgattgttg ttcaacatat
     2941 ttcttaatgg ctttaaccac ttcgtctgaa aatgtaagta cataagtatt gctgattgat
     3001 gtttgtggtt gatataaatc ttcaatcatt tccggcttaa cgtcataatg ctgatttaat
     3061 actttatctg aagcttcttt tttattgata aaacgcgtta caaatgacat atcttctcgc
     3121 attggttgac gaatttgttg attaacatgt tcttgtaacg catctgtcgc tgttaataat
     3181 cgctgttgtt gaatttgtag ctttttcttc tttttattaa aaaacccacc aacattaaag
     3241 tcttgagaca tactttccaa ataacttcgt aacatttctc tcatattatg cggcataatg
     3301 tatgcatttt ctaaaatatt tttacgctta ttctttaaaa atgccattaa ttcatctgga
     3361 ttatttaaaa gttgtgcctc ttcactaatt gcttgatgtt gttgactatt taaaaatgct
     3421 tgttcaaact ccgcttcttc gatacctaaa tcttctagta cttcctgaat ttcagactga
     3481 atgtaatcta gctgagcttc ggtaatgtat tcaaccgttc ttgatgtata atcctctatt
     3541 gtctctctat gttgatctaa tgaaattaga taacttgata atacttcaag ttcattttca
     3601 gggtgatcaa atttagatac ataaaaggtg cgttctaatt taatacccca atctgcaatt
     3661 gatttttcaa ctcgagattt aaacgtagag aatgacaatt catcgtcttg atgcttgtca
     3721 atttgattaa tgataaacac aacaggtatt ccaacatcat ttatatgctt cataaactta
     3781 aagttaagtt cagattgaac gtggttatag tcaaccgtat aaaatatcat attacttgta
     3841 tacatatatt gttctgttat tgactgatgt gatgcaacat ttgaatcaac acctggtgta
     3901 tcttgcaacg taaacccatt ttcaaattta gctgattgaa aattaatttc tacagattca
     3961 acgtcgacat tttggcgatt catttccctt acttcatcat aattagataa tttggcatac
     4021 gtttgattcg gcaaattagc aataatatcg tgattgtctg aaactgacac aatagcagta
     4081 ttacttgtcg ttggtacagg agaacttggt aagatatctt gttcaattaa taaatttatc
     4141 attgtcgatt tacctgcaga aaaatgtcca acgaacgaac atgtatattg ctgcaaatat
     4201 actttcttaa ttacttggtt aattgtatgt aaaagtgctt catttcgcga cttttcaact
     4261 tcttttttta atttatataa aagatctaat tgttctttat taatcatgtt actttcccct
     4321 ttggttcgtt cacatagaaa tgcttgcttg ttacaacgaa ttcatacaat atcgatgtcc
     4381 atttaaacat agatcgttaa atggctcgca ttgtattatg tttaaatttt atcatagctg
     4441 cttgataaaa catacgaata atcaattttc ttcaaacatt ccaacttaaa agttcatttt
     4501 ttaaaaatca caaatcatcg ttaaattttt ctagaaaata aatattgtca cagtttgttc
     4561 acacacgtca cagaattgac accgtataat agccaattgg gagggaaata tgaaaaatac
     4621 atttaatcca ttacaaagat tacatttcta tgcagcaata tttattgctc cactgttaat
     4681 cactttaacc atttcgggca ttggttactt gttctttcca gaagttgaaa ataatattta
     4741 taagaatgag ttttttggtg acagtgatgt aaaaacgcat caaacattaa atgatgcagt
     4801 acatcaagtt gaacaacaat atgaaggatt ctttgtaagt aaagttagca tacttgatga
     4861 accatataac aaacgaatta cactgagtga tatggcagga aatcaacgtt acgtctttct
     4921 agatcataac aatcaatttg ttgcagatca aaatgcgaaa catacgtatt ctaatgtgat
     4981 gcgaagtata catagttctt tgtttactga aaatactatt attaattatt tagtagagtt
     5041 aaccgcatgt tggatgatat tcatgatttt atctggtact tatttactca ttaagaagca
     5101 tttaatttct aacaaaagta aggcacttcg ttggcaaaag tggcacgcaa tgattggagt
     5161 tatcattgca attccagtat ttgtattagt cttaactgga ttgccatggt ctggttttat
     5221 gggcagtaaa attgccggta tgatggacac aaacggtgac cttggtcaag gtgaattagc
     5281 gattaatcca cctaaatcag atttgaacga attaccttgg gctacacgta aaaataaaca
     5341 gccagcttca tccgaaaaag gttcaagtgg tcatcatggt aatgcagcaa tgcctcaaac
     5401 caaattagat tatcaaatat ctattgataa ggtcgttgaa caggcgcaaa aagctggtat
     5461 taaaaagccg ttttcaatcg tatatccaag tgataaaaat ggtaccttta ttgtatctaa
     5521 tactagtaat tcaggtgtta ctgggctaga tgtatcacca tacaaggaac aaacacttta
     5581 tttcgatcaa tatagcggta aaaagctagg tacgattaaa tatgatgact acggtattat
     5641 tgctaaatgg tttacatggg gcattccgct tcacgaaggt catttattcg gcattttaaa
     5701 taaaatcatt aatttatttg tatgtatcgc tttattagta gccattggca tggggtttgt
     5761 ctcttggata aagcgtacaa aaaatactgc agtaaaagta ccacatcgcg taaaaaaacc
     5821 agcatctata tcact
//
LOCUS       DAMZKC010000146         5814 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.148, whole genome shotgun sequence.
ACCESSION   DAMZKC010000146 DAMZKC010000000
VERSION     DAMZKC010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5814
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.148"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            52..1620
                     /locus_tag="POB55_002128"
     CDS             52..1620
                     /locus_tag="POB55_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HDI6501083.1"
                     /translation="MIEKLVTFLNEVVWSKPLVYGLLITGVLFTLRMRFFQVRHFKEM
                     IRLMFQGEKSPNGISSFQAIAMSLAGRVGTGNIVGVSTAIFIGGPGAVFWMWITAFLG
                     ASSAFIESTLGQIFKRVENNEYRGGPAYYIEYGIGGKFGKIYGIIFAIVTIISVGLLL
                     PGVQSNAIASSMHNAIHVPQWLMGAIVVVILGLIIFGGVRSIANVATAVVPFMAIIYI
                     LMAVIIICINIQEVPALFALIFKSAFGLQSAFGGIVGAMIEIGVKRGLYSNEAGQGTG
                     PHAAAAAEVSHPSKQGLVQAFSVYIDTLFVCTATALIILISGTYNVTDGTVNANGTPH
                     LIKDGGIYVENATGKDYSGTAMYAQAGIDKAFHGSGYQFDPTFSGVGSYFIAFALFFF
                     AFTTILSYYYITETNVAYLTRNQNNQVSSIFINIARVIILFATFYGAVKTADVAWAFG
                     DLGVGLMAWLNIIAIWILHKPAVNALKDYEIQKKRLGNGYNAVYQPDPNKLPNAVFWL
                     KTYPERLKQARAKK"
     gene            1765..2523
                     /locus_tag="POB55_002129"
     CDS             1765..2523
                     /locus_tag="POB55_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDI6501084.1"
                     /translation="MSEFKTGKINKHVLYSNILNRDVTISIYLPESYNQLVKYNVILC
                     FDGLDFLRFGRIQRTYESLIKEARIDDAIIVGFHYEDVDKRREEFHPQGSRSHLTIQS
                     VGKEILPFIDSTFSTLKVGNARLLVGDSLAGSIALLTALTYPTIFSRVAMLSPHSDDK
                     VLDKLNQCANKEQLTIWHVIGLDEKDFTLPTNGKRADFLTPNRELAEQIKKYNITYYY
                     DEFEGGHQWKDWKPLLSDILLYFLRKNTDDQHYE"
     gene            2717..3226
                     /locus_tag="POB55_002130"
     CDS             2717..3226
                     /locus_tag="POB55_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcG family protein"
                     /protein_id="HDI6501085.1"
                     /translation="MILGLALIPSKSFQEAVDSYRKRYDKQYSRIKPHVTIKAPFEIE
                     DGDLDSVIEQVRARINGIPAVEVHATKASSFKPTNNVIYFKVAKTDDLEELFNRFNGE
                     DFYGEAEHVFVPHFTIAQGLSSQEFEDIFGQVALAGVDHKEIIDELTLLRFDDDEDKW
                     KVIETFKLA"
     gene            complement(3339..4529)
                     /locus_tag="POB55_002131"
     CDS             complement(3339..4529)
                     /locus_tag="POB55_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6501086.1"
                     /translation="MQDSSLNNYANHKNFILMLIILFLMEFARGMYILSYINFLPTVT
                     SIAVAITSLAFSIHFIADASTNFVIGFLLKKFGTKIVLTTGFILAFTSLFLVIWFPAS
                     PFVIIFSAMMLGIAVSPIWVIMLSSVEEDKRGKQMGYVYFSWLLGLLVGMVFMNLLIK
                     VHPTRFAFMMSLVVLIAWILYYFVDVKLTNYNTRPVKAQLRQIVDVTKRHLLLFPGIL
                     LQGAAIAALVPILPTYATKVINVSTIEYTVAIIIGGIGCAVSMLFLSKLIDNRSRNFM
                     YGVILSGFILYMILIFTLSMIVNIHIVWIIALAIGLMYGILLPAWNTFMARFIKSDEQ
                     EETWGVFNSIQGFGSMIGPLFGGLITQFTNNLNNTFYFSALIFLVLAVFYGSYFIANR
                     EKAK"
     gene            complement(4507..5682)
                     /locus_tag="POB55_002132"
     CDS             complement(4507..5682)
                     /locus_tag="POB55_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diglucosyl diacylglycerol synthase"
                     /protein_id="HDI6501087.1"
                     /translation="MVTQNKKILIITGSFGNGHMQVTQSIVNQLNDMNLDHLSVIEHD
                     LFMEAHPILTSICKKWYINSFKYFRNMYKGFYYSRPDKLDKCFYKYYGLNKLINLLIK
                     EKPDLILLTFPTPVMSVLTEQFNINIPVATVMTDYRLHKNWITPYSTRYYVATKETKQ
                     DFIDVGIDPSTVKVTGIPIDNKFETPINQKQWLIDNNLDPDKQTILMSAGAFGVSKGF
                     DTMITDILAKSANAQVVMICGKSKELKRSLTAKFKSNENVLILGYTKHMNEWMASSQL
                     MITKPGGITITEGFARCIPMIFLNPAPGQELENALYFEEKGFGKIADTPEEAIKIVAS
                     LTNGNEQLTNMISTMEQDKIKYATQTICRDLLDLIGHSSQPQEIYGKVPLYARFFVK"
BASE COUNT         1938 a          863 c         1032 g         1981 t
ORIGIN      
        1 ctcgtgctta atcgtataat atttttatgt ttaaacaata gggggaatct tatgattgaa
       61 aaattagtaa cctttttaaa tgaggttgta tggagtaagc cattagttta tggtttgcta
      121 attactggtg tgctatttac attgcgtatg cgattttttc aagttagaca ttttaaagaa
      181 atgattcgat taatgtttca aggagagaag tctcctaatg gtatttcaag ttttcaagcg
      241 atagccatgt ctttagcagg cagggttggt acaggtaata ttgtcggtgt atctactgca
      301 atatttatag gaggacctgg tgcagtattt tggatgtgga ttactgcgtt tttaggtgca
      361 agtagtgctt ttattgaatc tacacttggt caaatattca agagagttga aaataatgaa
      421 taccgtggtg gaccagcgta ttatattgaa tatggtattg gtggtaaatt tggtaaaatt
      481 tacggaatta tctttgctat tgttacgatt atctcagtag gtctattgct tcctggtgtg
      541 caatctaacg ctatagcaag ttctatgcat aatgcgattc atgttccaca atggttaatg
      601 ggtgctattg ttgtagttat tttgggatta attatttttg gtggtgtacg tagtattgcc
      661 aatgttgcaa cagccgttgt accatttatg gcaattattt acatactgat ggctgtcatt
      721 atcatttgta tcaatataca agaagtgcca gcgttatttg cattaatttt caaatcagca
      781 tttggattac aatctgcttt tggtggtatc gttggcgcaa tgatagagat tggtgttaaa
      841 cgtggattat actcaaatga ggctggtcaa ggtacaggtc cacacgcagc agcggcagca
      901 gaagtatcac atccaagtaa acaaggtcta gtacaagcat tttcagttta tattgataca
      961 ttatttgtat gtactgcaac tgctctgatt atacttattt ctggtacata taatgtgact
     1021 gatggtacgg ttaatgcgaa tggcacaccg catttaatta aagatggcgg tatttatgtt
     1081 gaaaatgcaa caggtaaaga ttattcaggt actgcgatgt atgcacaagc cggcattgat
     1141 aaagcgttcc atggcagtgg ttatcaattt gatcctactt tctctggcgt aggttcgtac
     1201 tttattgcat ttgctttatt cttctttgca tttactacaa ttttgtcgta ctactacatt
     1261 acagaaacaa atgttgctta tttaacgcgt aatcaaaata atcaagtttc atcgatattt
     1321 attaatattg ctcgtgtgat tattttgttc gctacatttt acggtgcagt taaaacagct
     1381 gatgtagcat gggcattcgg tgatttaggt gtaggtctaa tggcttggtt aaatatcatt
     1441 gcgatttgga ttttacataa gcctgccgta aatgctttaa aagattatga aattcaaaag
     1501 aaacgtttag gaaacggtta taatgcagtt tatcaacctg atccgaataa attacctaat
     1561 gctgtctttt ggttgaagac atatccagaa cgtttaaaac aagcacgtgc caaaaagtaa
     1621 tctacttttg tttatagtat atgtagtgat catttgataa aaaaagaaac agcattgata
     1681 attttaggtg ctcagaaatt tgaattttaa aaatatagtg tctcttggta caataacaat
     1741 acaactacta gggggcactt ttttatgtca gaatttaaaa ctggtaagat taataaacat
     1801 gttttatata gtaatatttt aaatagagat gtcacgataa gtatttattt accagaatct
     1861 tataatcaac ttgttaaata taatgtcatt ctttgctttg acggattaga ttttttacgt
     1921 ttcgggagaa tacaacgtac atatgaatcg ttaatcaaag aagcgcgtat tgatgatgcg
     1981 atcattgttg gattccatta tgaagacgtt gataagcgta gagaggaatt tcatccacaa
     2041 ggaagtcgtt ctcatttaac tattcaatca gtcggtaaag aaatattgcc atttattgac
     2101 tcgacgtttt ctacactgaa agtaggtaat gcaaggttat tagtagggga tagtttagcg
     2161 ggtagtattg ccttattaac ggcgttgacc tatccaacga tttttagtcg tgtagcaatg
     2221 ttaagtccac attcagatga caaagtatta gataagctaa atcaatgtgc taataaagaa
     2281 caattgacaa tttggcatgt cattggtcta gatgaaaaag attttacttt accaacaaat
     2341 ggtaagcgtg ccgatttctt aacaccgaat agagaattag ctgaacaaat taagaaatat
     2401 aatataactt attattacga tgaatttgaa ggtggtcacc aatggaaaga ttggaaacca
     2461 ttgctgtcag atatattatt gtatttttta cgtaaaaaca cagatgatca acattatgag
     2521 taatttacat tagtagattt agtatgaatt gtcttcatat agtttggtct ataatataat
     2581 ttataaaaga ttttactgtt tgatttaatt taaatttgtc gaaattgcaa aagatgtata
     2641 atgaattatt tttaatgtaa cggttttcaa agaaatttga tataatagca ataggttaaa
     2701 caaaggagga attcagatga ttttaggatt agcattaatt ccatcaaagt catttcaaga
     2761 agcggtggat tcttaccgta aaagatatga taaacagtat tcacgaatta aaccacatgt
     2821 gacaattaaa gcgccatttg aaattgaaga tggtgattta gattctgtca ttgaacaggt
     2881 tagagctcgt attaatggta taccagcagt agaagttcat gctacaaaag cttctagctt
     2941 caaaccaacg aacaatgtga tttactttaa agttgcgaag acggacgact tagaagaatt
     3001 gtttaatcgc tttaatggag aagatttcta tggagaagct gaacatgttt ttgtgccaca
     3061 ctttacaata gcacaaggac tatctagcca agaattcgaa gatatttttg gtcaagtagc
     3121 attagctggg gtagaccata aagaaattat cgatgaatta actttgttac gttttgacga
     3181 tgacgaagat aaatggaaag ttattgaaac gtttaaatta gcttaagtaa cataatagta
     3241 ttgttaatcg tagtatgttt gaattaataa gaaaatggtc atttttattg aatgtaataa
     3301 aaatgaccat tttctttatt ttaaaatacg ttttaacctt acttagcttt ttctctattt
     3361 gctataaagt agcttccata aaatacagct aagactaaaa agattaatgc cgagaaataa
     3421 aatgtattgt ttaaattgtt ggtaaattgt gtaattaatc cgccaaataa tggccctatc
     3481 attgagccga atccttggat actattaaaa acaccccaag tttcttcttg ttcatctgat
     3541 ttgataaatc gtgccataaa ggtattccat gctggtaata agatgccata cattagaccg
     3601 atagctaaag cgataatcca cacgatgtga atattaacaa tcatagatag agtgaaaatt
     3661 aatatcatgt ataaaataaa tccgcttaga ataacaccat acataaagtt tctgctgcgg
     3721 ttatctatta gtttcgataa aaatagcatc gaaactgcac agccgatacc accaataatg
     3781 attgcaacag tatattcaat tgtacttacg ttaataacct tagtagcata tgttggtaat
     3841 ataggaacta gggcagcaat tgcggcacct tgtaaaagaa taccagggaa caacaataaa
     3901 tggcgctttg tcacatcaac aatttgtctc aattgagctt taactggacg agtattataa
     3961 tttgttaact ttacatcgac aaaataatat aatatccatg caattaaaac gactaaagac
     4021 atcatgaagg caaagcgtgt tgggtgcact ttgataagta gattcataaa aaccatacct
     4081 accaataggc ctaacaacca tgaaaaataa acatagccca tttgtttgcc acgtttatct
     4141 tcttcaacac tggataacat aatgacccaa ataggactaa ctgcaatacc gagcatcata
     4201 gcactaaata tgattacaaa aggtgatgct ggaaaccaaa taactaaaaa taaacttgta
     4261 aatgctaaaa taaatccagt cgttaaaacg atttttgtgc cgaatttttt cagtaaaaat
     4321 cctataacaa agtttgtaga tgcatcagca ataaaatgta ttgaaaatgc tagagacgtt
     4381 attgctacag caatggatgt aactgttggc aagaaattaa tatagcttag gatatacatg
     4441 cctctcgcaa attccattaa aaataagata ataagcatta aaatgaaatt tttatgatta
     4501 gcgtaattat ttaacgaaga atcttgcata taaaggaacc tttccataaa tctcttgtgg
     4561 ttgtgatgaa tgaccgatta aatcaagtaa gtctcgacat attgtctgtg tagcatactt
     4621 aattttatct tgttccattg tactaatcat attagttaat tgctcattac cgttcgttaa
     4681 acttgctaca atttttattg cttcttctgg agtatcagcg attttaccaa aacctttttc
     4741 ttcaaagtaa agggcatttt caagctcttg accaggtgca ggatttagga aaatcattgg
     4801 aatacaacgg gcgaaacctt cagttattgt gataccacca ggtttcgtaa tcataagttg
     4861 acttgatgcc atccattcat tcatgtgttt ggtataacct agaatcaata cattctcgtt
     4921 agatttaaac ttagctgtta aagaacgctt tagctctttg ctcttaccac aaatcataac
     4981 tacttgtgca tttgcacttt tcgctaatat atcagtgatc atcgtgtcaa aacctttaga
     5041 tacaccaaat gcaccagctg acattaaaat agtttgctta tctggatcta agttgttgtc
     5101 tattaaccac tgcttttgat taataggcgt ttcaaatttg ttatcaatag gaatacctgt
     5161 cactttaact gttgaaggat caatacctac gtctatgaag tcttgtttcg tttcttttgt
     5221 tgccacataa tatcttgttg aatacggcgt aatccagttt ttatgtaagc gatagtctgt
     5281 catcactgta gcaactggaa tattaatgtt aaattgctca gtgagtaccg acataactgg
     5341 tgtaggaaac gttaataata ttaaatctgg cttttctttt atcaataaat tgattaactt
     5401 attaagtcca tagtatttgt aaaaacattt gtctagttta tctgggcggc tgtaataaaa
     5461 ccctttgtac atatttctaa aatatttaaa gctattgata taccattttt tacaaataga
     5521 agtcaaaatt ggatgagctt ccataaataa atcgtgctca atgacgctta aatggtctag
     5581 attcatatca ttaagttgat taacgatact ctgtgtaact tgcatatgac cgttaccgaa
     5641 tgagccagta ataatcaata tctttttatt ttgagtaacc attaatagcc accctccatt
     5701 agtttgaaaa ttttatttaa gtgtaactta ttttacggca ttataaaaga aataaagacg
     5761 caaagtcgtt acatttatag caattttaat caatagatga attgatacaa aata
//
LOCUS       DAMZKC010000147         5785 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.149, whole genome shotgun sequence.
ACCESSION   DAMZKC010000147 DAMZKC010000000
VERSION     DAMZKC010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5785
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.149"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            41..736
                     /locus_tag="POB55_002133"
     CDS             41..736
                     /locus_tag="POB55_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL7"
                     /protein_id="HDI6501088.1"
                     /translation="MKLKTLAKATLALGLLTTGVITSEGQAVQAKEKQERVQHLYDIK
                     DLHRYYSSESFEFSNISGKVENYNGSNVVRFNQENQNHQLFLSGKDKDKYKEGLEGQN
                     VFVVKELIDPNGRLSTVGGVTKKNNQSSETNTPLFIKKVYGGNLDASIESFLINKEEV
                     SLKELDFKIRQHLVKNYGLYKGTTKYGKITFNLKDGEKQEIDLGDKLQFEHMGDVLNS
                     KDIQNIAVTINQI"
     gene            1075..1773
                     /locus_tag="POB55_002134"
     CDS             1075..1773
                     /locus_tag="POB55_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL8"
                     /protein_id="HDI6501089.1"
                     /translation="MKFTAIAKAIFVLGILTTSVMITENQSVNAKGKYEKMNRLYDTN
                     KLHQYYSGPSYELTNVSGQSQGYYDSNVLLFNQQNQKFQVFLLGKDENKYKEKTHGLD
                     VFAVPELVDLDGRIFSVSGVTKKNVKSIFESLRTPNLLVKKIDDKDGFSYDEFFFIQK
                     EEVSLKELDFKIRKLLIKKYKLYEGAADKGRIVINMKDENKYEIDLSDKLGFERMADV
                     INSEQIKNIEVNLK"
     gene            2152..2850
                     /locus_tag="POB55_002135"
     CDS             2152..2850
                     /locus_tag="POB55_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL9"
                     /protein_id="HDI6501090.1"
                     /translation="MKFTALAKATLALGILTTGVFTTESKAVHAKVELDETQRKYYIN
                     MLHQYYSEESFEPTNISVKSEDYYGSNVLNFKQRNKAFKVFLLGDDKNKYKEKTHGLD
                     VFAVPELIDIKGGIYSVGGITKKNVRSVFGFVSNPSLQVKKIDPKHGFSINELFFIQK
                     EEVSLKELDFKIRKMLVEKYRLYKGASDKGRIVINMKDEKKYVIDLSEKLSFDRMFDV
                     MDSKQIKNIEVNLN"
     gene            3209..3892
                     /locus_tag="POB55_002136"
     CDS             3209..3892
                     /locus_tag="POB55_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL10"
                     /protein_id="HDI6501091.1"
                     /translation="MKLTAIAKAALALGILTTGTLTTEVHSGHAKQNQKSVNKHDKEA
                     LYRYYTGKTMEMKNISALKHGKNNLRFKFRGIKIQVLLPGNDKSKFQQRSYEGLDVFF
                     VQEKRDKHDIFYTVGGVIQNNKTSGVVSAPILNISKEKGEDAFVKGYPYYIKKEKITL
                     KELDYKLRKHLIEKYGLYKTISKDGRVKISLKDGSFYNLDLRSKLKFKYMGEVIESKQ
                     IKDIEVNLK"
     gene            4154..5710
                     /locus_tag="POB55_002137"
     CDS             4154..5710
                     /locus_tag="POB55_002137"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HDI6501092.1"
                     /translation="MSITEKQRQQQAELHKKLWSIANDLRGNMDASEFRNYILGLIFY
                     RFLSEKAEQEYADALAGEDITYQETWADEEYREDLKAELIDQVGYFIEPQDLFSAMIR
                     EIETQDFDIEHLATAIRKVETSTLGEESENDFIGLFSDMDLSSTRLGNNVKERTALIS
                     KVMVNLDDLPFVHSDMEIDMLGDAYEFLIGRFAATAGKKAGEFYTPQQVSKILAKIVT
                     DGKDKLRHVYDPTCGSGSLLLRVGKETQVYRYFGQERNNTTYNLARMNMLLHDVRYEN
                     FDIRNDDTLENPAFLGHTFDAVIANPPYSAKWTADSKFENDERFSGYGKLAPKSKADF
                     AFIQHMVHYLDDEGTMAVVLPHGVLFRGAAEGVIRRYLIEEKNYLEAVIGLPANIFYG
                     TSIPTCILVFKKCRQQEDYVLFIDASNDFEKGKNQNHLTDAQVERIINTYKRKETIDK
                     YSYSATLQEIADNDYNLNIPRYVDTFEEEAPIDLDQVQQDLKNIDKEIAEVEQEINAY
                     LKELGVLKDE"
BASE COUNT         2271 a          766 c         1055 g         1693 t
ORIGIN      
        1 attaagacaa aatttataaa tagattggga gaatagtact gtgaaattaa aaacgttagc
       61 taaagcaaca ttggcattag gcttattaac tactggtgtg attacatcag aaggccaagc
      121 agtgcaagca aaagaaaagc aagagagagt acaacattta tatgatatta aagacttaca
      181 tcgatactac tcatcagaaa gttttgaatt cagtaatatt agtggtaagg ttgaaaatta
      241 taacggttct aacgttgtac gctttaacca agaaaatcaa aatcaccaat tattcttatc
      301 aggaaaagat aaagataaat ataaagaagg ccttgaaggc cagaatgtct ttgtggtaaa
      361 agaattaatt gatccaaacg gtagactatc tactgttggt ggtgtaacga agaaaaataa
      421 ccaatcttct gaaactaata cacctttatt tataaaaaaa gtgtatggcg gaaatttaga
      481 tgcatcaatt gaatcatttt taattaataa agaagaagtt tcactgaaag aacttgattt
      541 caaaattaga caacatttag ttaaaaatta tggtttatat aaaggtacga ctaaatacgg
      601 taagatcact ttcaatttga aagatggaga aaagcaagaa attgatttag gtgataaatt
      661 gcaattcgag cacatgggcg atgtgttgaa tagtaaggat attcaaaata tagcagtgac
      721 tattaatcaa atttaaagta agtaatcaat gactctaaag taataaattt gaagcagctt
      781 agcgatgaaa tgttgaatag atacgtacac cttacataaa ggagcgtatt taaaacaacc
      841 ttgtcgttag gcttttttta cgttttataa cgcagggtat gagcgtacta aaaattcaca
      901 ttacttctga aagtgatgtc cattgaatat taattagttc ttcattaacc atgatttaat
      961 tttaattaaa cgagtgttaa tgtcagtctg tctcaatgcc ctttataata aatgtgtatt
     1021 attcaaatta cgtaataaaa gcaatccaat atattaagat tggagcatat gaatatgaaa
     1081 tttacagcga tagctaaagc gatatttgta ttaggaatat taacaacaag tgtaatgata
     1141 acagaaaatc aatcggttaa tgcaaaagga aagtatgaaa aaatgaaccg tttatatgat
     1201 acaaacaagt tacatcaata ctattcagga cctagttatg agttaacaaa tgttagtggc
     1261 caaagtcaag gttattatga ctctaacgtt ttgcttttta accaacaaaa tcaaaagttc
     1321 caagtgtttt tattgggaaa agatgaaaat aaatacaaag aaaaaacaca tggtttagat
     1381 gtctttgcgg taccggaatt agtagattta gatggaagaa tatttagtgt tagtggtgta
     1441 acaaagaaaa atgtaaaatc aatatttgag tctctaagaa cgccgaactt actagttaaa
     1501 aaaatagacg ataaagacgg tttttcgtat gatgaatttt tctttattca aaaggaagaa
     1561 gtatcattga aggaacttga tttcaaaata agaaaactgt taattaaaaa atacaaattg
     1621 tatgaagggg cagctgataa aggtagaatt gttattaata tgaaagatga aaataagtat
     1681 gaaattgatt taagtgataa attaggtttc gagcgtatgg cagatgtcat taatagtgaa
     1741 caaattaaaa acatcgaagt gaatttgaaa taatcaatga tatatataga atgaaagctt
     1801 aagaagcggt ttaataatcc catgtttaat gattttgata cgtgttttaa taataaaaac
     1861 atatcgaaca ttgactacgt tattaagctg cttttttgta cactttgtat cgaataactt
     1921 aagatctaaa actaatcgga aagaacaatg attcccctaa aaaaatttat gttgctatta
     1981 aaaatcagtt aatacgaatg ttaacatacg tttgattttc attaataatg attcaagttt
     2041 atttaaatga gcgttaatgt cagtctgttt tgatgcacct tataataaag acagatagtt
     2101 caaattacgt aataataaca atccaatata tcaagattgg agcaaataaa tatgaaattt
     2161 acggcattag caaaagcaac attagcatta ggaatattaa ctacaggtgt gtttacaaca
     2221 gaaagtaaag ctgttcacgc gaaagtagaa cttgatgaga cacaacgcaa atattatatc
     2281 aatatgctac atcaatacta ttctgaagaa agttttgaac caacaaatat tagtgttaaa
     2341 agcgaagatt actatggctc taacgtttta aactttaaac aacgaaataa agcttttaaa
     2401 gtatttttac ttggtgacga taaaaataaa tataaagaaa aaacacatgg ccttgatgtc
     2461 tttgcagtac ctgaattaat agatataaaa ggtggcatat atagcgttgg cggtataaca
     2521 aagaaaaatg tgagatcagt gtttggattt gtaagtaatc caagtctaca agttaaaaaa
     2581 atcgatccta aacatggctt ttcgataaat gagttgttct ttattcaaaa ggaagaagta
     2641 tcgttgaagg aactggattt taaaataaga aaaatgttag tcgaaaaata tagattgtat
     2701 aaaggcgcgt cagataaagg tagaatcgtt attaatatga aagacgaaaa gaaatatgta
     2761 attgatttaa gtgaaaaatt aagttttgat cgtatgtttg atgtaatgga tagtaagcaa
     2821 attaaaaata ttgaagtgaa tttgaattaa ttaagtataa taacttaaga agcgacttaa
     2881 cgacaaaatg tgaattgaca tgcatgtcct taaataagga actgtgttaa atacattact
     2941 gttgttaagt tgttttttgc gtttcaaaga gcagaacaga gtaacatcat cagttgtagt
     3001 aaacgataat ctagtaaaac aactaaatga aataatgaaa ttcatttaac ctgaacatta
     3061 aaatatattt gtttttcatt aagaataatt caagtatatt taaatcgagg ttaattatcg
     3121 tatgaaacga tgcacgttat aataaaaatg tatgattcaa attacgtaat gaaaacaatc
     3181 caatatatta agattggagc aaaacaatat gaaattaaca gcgatagcta aagctgcatt
     3241 agctttagga attttaacaa caggaacttt aacaacagaa gttcattcag gtcatgcaaa
     3301 acaaaatcaa aagtcagtaa ataaacatga caaggaagca ttataccgat actacactgg
     3361 aaagactatg gaaatgaaaa atattagtgc tttgaaacat ggtaaaaata acttgcgttt
     3421 taagtttaga ggtattaaga ttcaagtttt actgcctgga aatgataaaa gtaaatttca
     3481 acagcgtagt tatgaggggt tagatgtgtt ttttgttcaa gaaaaaagag ataagcacga
     3541 tatattttat actgttggtg gtgtaataca gaataataaa acatctggag ttgtcagtgc
     3601 accaatatta aatatttcaa aagaaaaggg tgaagatgct tttgtgaaag gttaccctta
     3661 ttacattaaa aaagaaaaaa taacactaaa agagctggat tataagttga gaaagcatct
     3721 aatcgaaaaa tatggacttt ataaaacaat ctcaaaagat ggtagggtca aaattagctt
     3781 gaaagatggc agtttttata accttgattt aagatctaaa ttaaaattca aatatatggg
     3841 ggaagtcata gaaagcaaac aaattaaaga tattgaagtt aacttaaagt aaataattac
     3901 gaataataaa aagtaattga agcggcttaa cgatgaaaag taaattgatg cgcatacctt
     3961 accaaaagga tgcatcaatc gatatcgtcg ttaagctgtt ttggtttacg tttcatggat
     4021 tctaccccaa ttttcataaa tataaaaatt ccaccaccaa catcaaaatt ctcaacatcg
     4081 caacataccc aaatgttata ataaatctat tacacaaaga gataaattac ttattcaaag
     4141 gcggaggaat cacatgtcta ttactgaaaa acaacgtcag caacaagctg aattacataa
     4201 aaaattatgg tcgattgcga atgatttaag agggaacatg gatgcgagtg aattccgtaa
     4261 ttacatttta ggcttgattt tctatcgctt cttatccgaa aaagcagaac aagaatatgc
     4321 agatgcgttg gcaggtgaag atatcacgta tcaagagaca tgggcagatg aagaatatcg
     4381 tgaagactta aaagctgaat taattgatca agtcggttac ttcattgaac cacaagattt
     4441 attcagtgcg atgattcgtg aaattgaaac gcaagatttc gatatcgaac atctggcgac
     4501 ggcaattcgt aaagttgaaa catcaacatt aggtgaagaa agtgaaaatg actttatcgg
     4561 actgttcagc gatatggact taagttcaac gcgactaggt aacaatgtca aagaacgtac
     4621 tgctttaatc tctaaagtca tggttaatct tgacgattta ccatttgttc acagtgatat
     4681 ggaaattgat atgttaggtg atgcatatga attcctaatt gggcgctttg cggcgacagc
     4741 aggtaaaaaa gcaggcgagt tctatacacc acaacaagta tctaagatac tggcgaagat
     4801 tgtcacagac ggtaaagata aattacgtca tgtgtacgac ccaacatgtg gttccggttc
     4861 attattgtta cgtgttggta aagaaacgca agtgtatcgt tatttcggtc aagaacgtaa
     4921 caataccact tacaacttag cacgcatgaa catgttatta catgatgtac gttatgaaaa
     4981 tttcgatatc cgtaatgatg acacgttgga aaatccagcc tttttaggac atacatttga
     5041 tgcggttatt gcgaacccac catacagtgc gaaatggaca gcagattcaa aatttgaaaa
     5101 tgacgaacga ttcagcggat acggcaaact tgcgccaaag tccaaagcag actttgcctt
     5161 tattcaacac atggtacatt acttagacga tgaaggtacc atggccgttg tactcccaca
     5221 tggtgtctta ttccgtggtg ctgcagaagg tgtcattcgt cgttatttaa ttgaagaaaa
     5281 gaactactta gaagccgtga ttggcttacc agccaatatt ttctatggga caagtattcc
     5341 aacatgtatt ttagtattta aaaaatgtcg ccaacaagaa gactatgtat tatttatcga
     5401 tgcatccaat gattttgaaa aaggaaaaaa tcaaaaccat ttaaccgatg cccaagtcga
     5461 acgcattatt aacacatata agcgtaagga aacaattgat aaatatagct acagtgcgac
     5521 attacaagag atcgccgata acgattacaa cttaaacata ccgagatatg tcgatacatt
     5581 cgaagaagaa gcaccgattg atttagatca agtccaacaa gatttgaaaa atatcgacaa
     5641 agaaatcgca gaagttgaac aagaaatcaa tgcatacctg aaagaacttg gggtgttgaa
     5701 agatgagtaa tacacaaaag aaaaatgtgc cagagttgag gttcccaggg tttgaaggcg
     5761 aatgggaaga gaagaagtta gggga
//
LOCUS       DAMZKC010000148         5722 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.150, whole genome shotgun sequence.
ACCESSION   DAMZKC010000148 DAMZKC010000000
VERSION     DAMZKC010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5722
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.150"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            108..1028
                     /gene="mvk"
                     /locus_tag="POB55_002138"
     CDS             108..1028
                     /gene="mvk"
                     /locus_tag="POB55_002138"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HDI6501093.1"
                     /translation="MTRKGYGESTGKIILIGEHAVTFGEPAIAVPFNAGKIKVLIEAL
                     ESGNYSSIKSDVYDGMLYDAPDHLKSLVNRFVELNNITEPLAVTIQTNLPPSRGLGSS
                     AAVAVAFVRASYDFLGKSLTKEELIEKANWAEQIAHGKPSGIDTQTIVSGKPVWFQKG
                     HAETLKTLSLDGYMVVIDTGVKGSTRQAVEDVHKLCEDPQYMSHVKHIGKLVLRASDV
                     IEHHNFEALADIFNECHADLKALTVSHDKIEQLMKIGKENGAIAGKLTGAGRGGSMLL
                     LAKDLPTAKNIVKAVEKAGAAHTWIENLGG"
     gene            1033..2016
                     /gene="mvaD"
                     /locus_tag="POB55_002139"
     CDS             1033..2016
                     /gene="mvaD"
                     /locus_tag="POB55_002139"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HDI6501094.1"
                     /translation="MIKSGKARAHTNIALIKYWGKKDEALIIPMNNSISVTLEKFYTE
                     TKVTFNDQLTQDQFWLNGEKVSGKELEKISKYMDIVRNRAGIDWYAEIESDNFVPTAA
                     GLASSASAYAALAAACNQALDMQLSDKDLSRLARIGSGSASRSIYGGFAEWEKGYSDE
                     TSYAVPLESNHFEDDLAMIFVVINQHSKKVPSRYGMSLTRNTSRFYQYWLDHIDEDLA
                     EAKAAIQDKDFKRLGEVIEENGLRMHATNLGSTPPFTYLVQESYDVMALVHECREAGY
                     PCYFTMDAGPNVKILVEKKNKQQIIDKLLTQFDNNQIIDSDIIATGIEIIE"
     gene            2029..3105
                     /locus_tag="POB55_002140"
     CDS             2029..3105
                     /locus_tag="POB55_002140"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HDI6501095.1"
                     /translation="MIQVKAPGKLYIAGEYAVTEPGYKSVLIALDRFVTATIEEADQY
                     KGTIHSKALHHNPVTFSRDEDSIVISDPHAAKQLNYVVTAIEIFEQYAKSCDIAMKHF
                     HLTIDSNLDDSNGHKYGLGSSAAVLVSVIKVLNEFYDMKLSNLYIYKLAVIANMKLQS
                     LSSCGDIAVSVYSGWLAYSTFDHEWVKHQIEDTTVEEVLIKNWPGLHIEPLQAPENME
                     VLIGWTGSPASSPHFVSEVKRLKSDPSFYGDFLEDSHHCVEKLIHAFKTNNIKGVQKM
                     VRQNRTIIQRMDKEATVDIETEKLKYLCDIAEKYHGASKTSGAGGGDCGITIINKDVD
                     KEKIYDEWTKHGIKPLKFNIYHGQ"
     gene            3282..3623
                     /locus_tag="POB55_002141"
     CDS             3282..3623
                     /locus_tag="POB55_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1450 domain-containing protein"
                     /protein_id="HDI6501096.1"
                     /translation="MKNTFLICDECQAVNIRTLQKKLEKLDPDAEIVIGCQSYCGPGR
                     RKTFTFVNNRPLAALTEEELIEKVSQQLKKPRDPEEEERLRKRHEERKRRKEEQDRKL
                     KEKLEKRKAQQ"
     gene            complement(3675..4997)
                     /locus_tag="POB55_002142"
     CDS             complement(3675..4997)
                     /locus_tag="POB55_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HDI6501097.1"
                     /translation="MKTYDLIVIGFGEAGKTLAKYAASTGQHVAVIEQSPKMYGGTCI
                     NIGCIASKTLVHDGLEGKSFEASYNRKNDVVNALNNKNYHLLADDNNIDVLDFKAQFK
                     SNTEVNLLDQHDDIADSITAPHIIINTGATSVIPNIKGLDQAKHVFDSTGLLNINYQP
                     KHLVIVGGGYIALEFASMFANLGSKVTVLERGESFMPREDQDVVAHAITDLENKGITL
                     HTNVETTELSSDNHHTTVHTNVGNFEADAVLLAIGRKPNTDLALENTDIELGDRGEIK
                     VNAHLQTTVPHIYAAGDVKGGLQFTYISLDDYRIIKSALYGNQSRTTDNRGGVPYTVF
                     IDPPLSRVGLTSKEAAAQHYDYTEHQLLVSAIPRHKINNDPRGLFKVVINNENNMILG
                     ATLYGKQSEELINIIKLAIDQNIPYTVLRDNIYTHPTMAESFNDLFNF"
     gene            complement(4984..5424)
                     /locus_tag="POB55_002143"
     CDS             complement(4984..5424)
                     /locus_tag="POB55_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDI6501098.1"
                     /translation="MNLEFNIAVHVLAFLTKHHSEKFNSSSLAELTCLNPVQLRRVTT
                     QLVDLKMINTIRGKDGGYLSNDRSADVSLATLYKHFVLEKEHHTRLFTGDEGSHCQIA
                     RNIATTMSHYEQDEQNIIINFYKGKTIKDVIEDIQKEDLCHENI"
     gene            5482..5583
                     /locus_tag="POB55_002144"
     CDS             5482..5583
                     /locus_tag="POB55_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501099.1"
                     /translation="MCQYLCLRTIELGVFIVMIYCLKVVALTVRLHN"
BASE COUNT         1962 a          852 c         1132 g         1776 t
ORIGIN      
        1 caatgaaatt taacgcgaat gcgtatgcaa cttagaatat gatgtctttt actaataaat
       61 aaaaataatg aatcaagtat aggataaata taaaaggtga tattgacatg acaagaaaag
      121 gatatgggga atcgacaggt aagattattt taataggaga acatgctgtt acatttggag
      181 agcctgctat tgcagtaccg tttaacgcag gtaaaatcaa agttttaata gaagccttag
      241 agagcgggaa ctattcgtct attaaaagcg atgtttacga tggtatgtta tatgatgcgc
      301 ctgaccatct taagtctttg gtgaaccgtt ttgtagaatt aaataatatt acagagccgc
      361 tagcagtaac gatccaaacg aatttaccac catcacgtgg attaggatcg agtgcagctg
      421 tcgcggttgc ttttgttcgt gcaagttatg attttttagg gaaatcatta acgaaagaag
      481 aactcattga aaaggctaat tgggcagagc aaattgcaca tggtaaacca agtggtattg
      541 atacgcaaac gattgtatca ggcaaaccag tttggttcca aaaaggtcat gctgaaacgt
      601 tgaaaacgtt aagtttagac ggctatatgg ttgttataga tactggtgtg aaaggttcaa
      661 caagacaagc agtagaagat gttcataaac tttgtgagga ccctcagtac atgtcacatg
      721 taaaacatat cggtaagtta gttttacgtg cgagtgatgt gattgaacat cataactttg
      781 aagccttagc ggatattttt aatgaatgtc atgcggattt aaaggcgttg acagttagtc
      841 atgataaaat agaacaatta atgaaaattg gtaaagaaaa tggtgcgatt gctggaaaac
      901 ttactggcgc tggtcgtggt ggaagtatgt tattgcttgc caaagattta ccaacagcga
      961 aaaatattgt aaaagctgta gaaaaagctg gtgcagcaca tacttggatt gagaatttag
     1021 gaggttaatg cgttgattaa aagtggcaaa gcacgtgcac atacgaatat tgcacttata
     1081 aaatattggg gtaaaaaaga tgaagcacta atcattccaa tgaataatag catatctgtt
     1141 acattagaaa aattttacac tgaaacgaaa gtcactttta acgaccagtt aacacaggat
     1201 caattttggt tgaatggtga aaaggttagt ggcaaagaat tagagaaaat ttcaaaatat
     1261 atggatattg tcagaaatag agctggcatc gattggtatg cagaaattga aagcgacaat
     1321 tttgtaccaa cagcagcagg gttggcttca tcggcaagcg catatgcagc tttagcagca
     1381 gcttgtaatc aagcgctaga catgcagctg tcagataagg atttatcgag attggcgcga
     1441 attggttcgg gttctgcgtc gcgtagtatt tatggtggat ttgcagaatg ggaaaaaggg
     1501 tatagtgatg agacgtcata tgccgttcca cttgaatcga atcattttga agatgacctt
     1561 gccatgatat ttgttgtgat taatcaacat tctaaaaagg tacctagtcg atatggtatg
     1621 tcattgacac gaaacacatc aaggttttat caatattggt tagatcatat tgatgaagat
     1681 ttagctgaag caaaagcagc gattcaagac aaagatttta aacgccttgg tgaagtaatt
     1741 gaagaaaatg gtttgcgtat gcatgccacg aatctaggat caacaccgcc gttcacatat
     1801 cttgtgcaag aaagttatga tgtcatggcg cttgttcacg aatgccgaga agcggggtat
     1861 ccgtgttatt ttacaatgga tgcgggacct aatgtgaaaa tacttgtaga aaagaaaaac
     1921 aagcaacaga ttatagataa attattaaca cagtttgata ataaccaaat tattgatagt
     1981 gacattattg ccacaggaat tgaaataatt gagtaaggaa gagataaaat gattcaggtc
     2041 aaagcacccg gaaaacttta tattgctgga gaatatgctg taacagaacc aggatataaa
     2101 tctgtactta ttgcgttaga tcgttttgta actgctacta ttgaagaagc agaccaatat
     2161 aaaggtacca ttcattcaaa agcattacat cataacccag ttacatttag tagagatgaa
     2221 gatagtattg tcatttcaga tccacatgca gcaaaacaat taaattatgt ggtcacagct
     2281 attgaaatat ttgaacaata cgcgaaaagt tgcgatatag cgatgaagca ttttcatctg
     2341 actattgata gtaatttaga tgattcaaat ggtcataaat atggattagg ttcaagtgca
     2401 gcagtacttg tgtcagttat aaaagtatta aatgaatttt atgatatgaa gttatctaat
     2461 ttatacattt ataaactagc agtgattgca aatatgaagt tacaaagttt aagttcatgc
     2521 ggagatattg ctgtgagtgt atatagtgga tggttagcgt atagtacttt tgatcatgaa
     2581 tgggttaagc atcaaattga agatactacg gttgaagaag ttttaatcaa aaactggcct
     2641 ggattgcaca tcgaaccatt gcaagcacct gaaaatatgg aagtacttat cggttggact
     2701 ggctcaccgg cgtcatcacc acactttgtt agcgaagtga aacgtttgaa atcagatcct
     2761 tcattttacg gtgacttctt agaagattca catcattgtg ttgaaaaact tattcatgct
     2821 tttaaaacaa ataacattaa aggtgtgcaa aagatggtgc gtcagaatcg tacaattatt
     2881 caacgtatgg ataaagaagc tacagttgat atagaaactg aaaagctaaa atatttgtgt
     2941 gatattgctg aaaagtatca cggcgcatct aaaacatcag gcgctggtgg tggagactgt
     3001 ggtattacaa ttatcaataa agatgtagat aaagaaaaaa tttatgatga atggacaaaa
     3061 catggtatta aaccattaaa atttaatatt tatcatgggc aataaatgat ttgatagggg
     3121 agcaatcatc taatttaatt agaagataaa tgctccattt tttattgcca gccgtgttac
     3181 gtatcttgaa aaaaatatta aaaagagtaa aatagataga gttaacgaaa aaattaatga
     3241 aatcgacgat atagatatga taaagaaagg tgggtagcaa tatgaaaaat acattcctta
     3301 tttgtgatga atgtcaggca gtcaatataa gaacgttaca aaagaagttg gaaaaattag
     3361 atcccgatgc tgaaatcgtg ataggttgtc aatcttattg tggacctgga cgccgaaaaa
     3421 cattcacttt tgttaataac cgcccactgg ctgcgcttac tgaagaagaa ttaatcgaaa
     3481 aagtttctca acaattaaag aaaccacgtg atcctgaaga agaagagcgt ttaagaaaac
     3541 gacatgaaga acgtaaacgt cgtaaagaag aacaagatag aaagcttaaa gaaaaattag
     3601 aaaagcgaaa agcacaacaa taaagcctga tggcagcatc attcaatgcg tgccaccagg
     3661 tttttatgtt ttgtctagaa attaaataaa tcattaaatg attcggccat cgtaggatgc
     3721 gtataaatat tatctcgtaa tacggtatat ggaatgtttt gatcaatcgc aagtttaatt
     3781 atattaatta attcttcaga ttgcttacca tataatgtag cacctaaaat catattattt
     3841 tcattattaa tgactacttt aaataaacct cttggatcat tgttaatttt gtgacgaggt
     3901 atagcactta ctaaaagttg atgttcagtg taatcataat gttgagcggc agcttcttta
     3961 ctagttaatc caacacgtga taatggtgga tctataaata ctgtataagg cacgccacct
     4021 ctattgtcag tcgtacgtga ctgattacca tataacgctg atttgataat tcgataatcg
     4081 tctaaagata tatacgtaaa ttgaagtccg cctttaacat cacctgcagc ataaatatgc
     4141 ggtacagttg tttgaagatg agcattgact ttaatttcgc ctctgtcgcc taattcgata
     4201 tcagtatttt ctaaagctaa atccgtattc ggtttgcgcc cgatagccaa aagtactgca
     4261 tcagcctcaa agttaccaac gttggtatgg actgttgtat gatgattgtc agatgacaat
     4321 tcagtcgttt caacatttgt atgcaatgta atgcctttat tttctaagtc agtaatagca
     4381 tgtgcaacga catcttgatc ttcacgtggc ataaatgatt caccacgttc taatactgta
     4441 accttactac ctaaattcgc aaacattgaa gcaaattcta aggcgatata accgccacct
     4501 acaataacga ggtgcttagg ttgatagtta atgtttaata aacctgttga atcgaagacg
     4561 tgtttagctt gatcaaggcc tttaatgtta ggaataacag aggtagcacc agtattaata
     4621 atgatatgag gtgcagtaat actatcggcg atatcgtcat gctgatctaa taaattcact
     4681 tcagtattag atttaaactg cgctttaaaa tccagtacat caatgttgtt atcgtctgct
     4741 aataaatggt aatttttatt gtttagcgca ttgacaacat cgtttttacg gttataactt
     4801 gcttcaaaag atttgccttc taatccatca tgtacaagtg tctttgaagc aatacatcct
     4861 atgtttatac aagtgcctcc atacattttc ggagattgtt cgataactgc gacgtgttga
     4921 cctgttgatg cagcatattt ggctaaagtt ttaccagctt ccccaaatcc tattacaatt
     4981 aaatcatatg ttttcatgac ataaatcctc cttttgaatg tcttcaatga catctttgat
     5041 tgtttttcct ttataaaaat taataatgat attctgttcg tcttgctcat aatgtgacat
     5101 agtagttgca atattacgag caatttgaca gtgactgcct tcgtcgccag taaatagtcg
     5161 tgtgtgatgt tctttctcta agacaaaatg tttatataat gttgctagag agacatcagc
     5221 gctacgatca tttgataaat aaccaccatc tttaccccgt attgtgttaa tcatttttaa
     5281 atcgacaagt tgagtcgtca cgcgtcgtaa ttgaacagga tttaaacaag ttaattctgc
     5341 taatgaacta ctattgaatt tttctgaatg atgcttagtt aaaaaagcta atacatgcac
     5401 ggcaatgtta aattctaaat tcaatgtttc tcacctcata attgtaacta tattagttac
     5461 aattaaaaat cattttgctc aatgtgtcaa tacttatgtt tgagaactat tgaattaggt
     5521 gtgtttattg tgatgattta ttgtttgaaa gttgttgcat taacggtgag attgcacaat
     5581 tgaaatttta gttatgtatg aactgataga tatgaaatga atattgaaaa taagggcaat
     5641 aaaattttga atgatgatgt tgtgtgattg tataaactta ttcgtatttt agatacgttt
     5701 tgatatttcg catgatgagg aa
//
LOCUS       DAMZKC010000149         5698 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.151, whole genome shotgun sequence.
ACCESSION   DAMZKC010000149 DAMZKC010000000
VERSION     DAMZKC010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5698
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.151"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(89..177)
                     /locus_tag="POB55_002145"
     tRNA            complement(89..177)
                     /locus_tag="POB55_002145"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(140..142),aa:Ser,seq:gga)
     gene            complement(232..303)
                     /locus_tag="POB55_002146"
     tRNA            complement(232..303)
                     /locus_tag="POB55_002146"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(268..270),aa:Glu,seq:ttc)
     gene            complement(305..379)
                     /locus_tag="POB55_002147"
     tRNA            complement(305..379)
                     /locus_tag="POB55_002147"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(345..347),aa:Asn,seq:gtt)
     gene            complement(394..467)
                     /locus_tag="POB55_002148"
     tRNA            complement(394..467)
                     /locus_tag="POB55_002148"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(433..435),aa:Gly,seq:tcc)
     gene            complement(483..558)
                     /locus_tag="POB55_002149"
     tRNA            complement(483..558)
                     /locus_tag="POB55_002149"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(523..525),aa:His,seq:gtg)
     gene            complement(572..644)
                     /locus_tag="POB55_002150"
     tRNA            complement(572..644)
                     /locus_tag="POB55_002150"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(609..611),aa:Phe,seq:gaa)
     gene            complement(661..736)
                     /locus_tag="POB55_002151"
     tRNA            complement(661..736)
                     /locus_tag="POB55_002151"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(701..703),aa:Asp,seq:gtc)
     gene            complement(758..831)
                     /locus_tag="POB55_002152"
     tRNA            complement(758..831)
                     /locus_tag="POB55_002152"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(795..797),aa:Met,seq:cat)
     gene            complement(1301..1855)
                     /locus_tag="POB55_002153"
     CDS             complement(1301..1855)
                     /locus_tag="POB55_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase family protein"
                     /protein_id="HDI6501100.1"
                     /translation="MTDVIIVHSMHGNSRNHWYQWLEHNLTLEGYDVTLFNFESPEAK
                     TVDQWIEAMTKQINVRKKDTYFVTHGLGSITALKYIEMIDQPIEGFFSIAGFKEDAEN
                     IDLDIDLSNVTIDYDNIKKKVDNFYGLSSKNDKYVSYKETQRLMNALEGNLRIVEDGG
                     HFLEEDGFETFTALQDRMQDYMTR"
     gene            complement(2177..3577)
                     /locus_tag="POB55_002154"
     CDS             complement(2177..3577)
                     /locus_tag="POB55_002154"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HDI6501101.1"
                     /translation="MTKSVAIIGAGITGLSSAYFLKQQDPNIDVTIFEASNRPGGKIQ
                     SYRKDGYMIELGPESYLGRKTIMTELAKDIGLEQDIVTNTTGQSYIFAKNKLYPIPGG
                     SIMGIPTDIKPFVTTKLISPLGKLRAGLDLIKKPIQMQDGDISVGAFFRARLGNEVLE
                     NLIEPLMGGIYGTDIDKLSLMSTFPNFKEKEEAFGSLIKGMKDEKNKRLKQRQLYPGA
                     PKGQFKQFKHGLSSFIEALEQDVKNKGVTIRYNTSVDDIITSQKQYKIVYSNQQEDVF
                     DGVLVTTPHQVFLNWFGQDPAFDYFKTMDSTTVATVVLAFDEKDIENTYDGTGFVIAR
                     TSDTDITACTWTSKKWPFTTPEGKVLIRAYVGKPGDTVVDDHTDNELVSIVRRDLSQM
                     MTFKGDPEFTIVNRLPKSMPQYHVGHIQQIRQIQAHIKQTYPRLRVTGASFEAVGLPD
                     CITQGKVAAEEVIAEL"
     gene            complement(3601..4524)
                     /gene="hemH"
                     /locus_tag="POB55_002155"
     CDS             complement(3601..4524)
                     /gene="hemH"
                     /locus_tag="POB55_002155"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HDI6501102.1"
                     /translation="MTKKMGLLVMAYGTPYKESDIEPYYTDIRHGKRPSEEELQDLKD
                     RYEFIGGLSPLAGTTDDQADALVSALNKAYADVEFKLYLGLKHISPFIEDAVEQMHND
                     GITEAITVVLAPHYSSFSVGSYDKRADEEAAKYGIQLTHVKHYYEQPKFIEYWTNKVN
                     ETLAQIPEEEHKDTVLVVSAHSLPKGLIEKNNDPYPQELEHTALLIKEQSNIEHIAIG
                     WQSEGNTGTPWLGPDVQDLTRDLYEKHQYKNFIYTPVGFVCEHLEVLYDNDYECKVVC
                     DDIGANYYRPKMPNTHPLFIGAIVDEIKSIF"
     gene            complement(4582..5619)
                     /gene="hemE"
                     /locus_tag="POB55_002156"
     CDS             complement(4582..5619)
                     /gene="hemE"
                     /locus_tag="POB55_002156"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HDI6501103.1"
                     /translation="MVHNKNNTILKMIKGEETSHTPVWFMRQAGRSQPEYRKLKEKYS
                     LFDITHQPELCAYVTHLPVDNYHTDAAILYKDIMTPLKPIGVDVEIKSGIGPVIHNPI
                     KTIQDVEKLSQIDPERDVPYVLDTIKLLTEEKLNVPLIGFTGAPFTLASYMIEGGPSK
                     NYNFTKAMMYRDEATWFALMNHLVDVSVKYVTAQVEAGAELIQIFDSWVGALNVEDYR
                     RYIKPHMIRLISEVKEKHDVPVILFGVGASHLINEWNDLPIDVLGLDWRTSINQAQQL
                     GVTKTLQGNLDPSILLAPWNVIEERLKPILDQGMENGKHIFNLGHGVFPEVQPETLRK
                     VSEFVHTYTQR"
     gene            complement(5609..>5698)
                     /locus_tag="POB55_002157"
     CDS             complement(5609..>5698)
                     /locus_tag="POB55_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501104.1"
                     /translation="DIYLLKIRTNERYDNIDKNDFNLGGLYGA"
BASE COUNT         1732 a         1172 c          847 g         1947 t
ORIGIN      
        1 ataatagctc aaatgcagat atatcaaggg tttgagtcta aaataaaaat agcgcttaaa
       61 atgggtaaaa ccaatttaag cgcctttacg gaaggtaagg gattcgaacc cttgagacgc
      121 ttgtgacgcc tacacacttt ccaggcgtgc tccttcggcc agctcggaca accttcctca
      181 acaataaata tgtaataaaa aaacagaagc gatatttcac ttctgtttgt atgactccta
      241 cgggactcga acccgtgtta ccgccgtgaa agggcggtgt cttaaccgct tgaccaagga
      301 gccatggctc cacaggtagg actcgaacct acgaccgatc ggttaacagc cgatagctct
      361 accactgagc tactgtggaa taatagaaaa taatggagcg ggtgatggga atcgaaccca
      421 caacatcagc ttggaaggct gaggttttgc cattaaacta cacccgctaa tgtaaacgat
      481 tatggggcgg ctgaagggga tcgaaccctc gaatgtcgga accacaatcc gatgtgttaa
      541 ccacttcacc acaaccgcca tgaattaaga atggttcagg acagagtcga actgccgaca
      601 catggagctt caatccattg ctctaccaac tgagctactg aaccttaatg gtaataataa
      661 tggcggtctc gacgggaatc gaacccgcga tctcctgcgt gacaggcagg cgtgttaacc
      721 gctacactac gagacctatt aaattaaaaa ctatgtattg cgggaggcgg atttgaacca
      781 ccgaccttcg ggttatgagc ccgacgagct accgaactgc tccatcccgc gctaatatta
      841 ttttgaatta cctaattaat atatcataat caaaaaccta aagtcaagaa ctttttgaat
      901 ttaatttaaa tgttatctct taaataatta cttaaatatc gtagcaacat gttctctgtt
      961 gaacacaaat attagtatat tcatttttgt agtatacgtc aacgacattt ttaaagtttt
     1021 ttgtgtaaaa aaacgcttct tattcccttt tatcatataa gtgtctaata gttgtcataa
     1081 atagtgttaa agcatttaaa aaggtatagg agttataaag tttacaacgc ctataccttc
     1141 tgaaaaagaa attataaaag cttgttacac cgcatattct ttcagtcagc gactaccaat
     1201 ataacattgt agccctaaga cattgcttga cgcctcaatt accacaattt tttcaaaatc
     1261 agcagctacc tactgacaca acatagcaac caaacattac ctatcgtgtc atgtaatctt
     1321 gcatccgatc ttgcaacgct gtaaatgttt cgaagccatc ctcttctaag aagtgccctc
     1381 catcttccac gattcgcaag ttcccctcta atgcattcat taaacgctgg gtttctttat
     1441 atgaaacgta tttgtcattt ttagaactca atccgtaaaa attgtcaact ttctttttaa
     1501 tattatcgta atcaatggtt acattactta aatcaatatc taaatctata ttttctgcat
     1561 cttctttaaa gcccgctata ctaaaaaagc cttcaatcgg ctgatcaatc atttcaatat
     1621 attttaaagc tgtgattgaa cctaaaccat gtgttacaaa atatgtatcc tttttgcgta
     1681 cattaatttg tttcgtcata gcttcaatcc actgatccac tgtcttcgct tcaggggatt
     1741 caaaattaaa taatgttacg tcatatcctt ctaaagttaa gttatgctcc aaccactgat
     1801 accaatgatt tctactattt ccatgcatag aatgtacaat aattacatct gtcatctcat
     1861 tctctccttt caacttacta cttcttttct atttttaaaa aaatgactga ttacctataa
     1921 ttgtaaaata aaaacacctt aattagaaat gttatatcgc aaagtgacat ttctaattaa
     1981 agtgtattgt catcatttca atatcattca aaaacagcta aacctttgtc tctgcttcaa
     2041 attcacaaaa ataattcccg ttgaaagtat ctatatttac acattacttc caccattata
     2101 taacttaaaa atgactatat ttcatcaaac attatctaaa ggcgtcgcac ctacaccaca
     2161 ccatccaaca attaacttac aactctgcga ttacttcttc agcagcaact ttaccttgcg
     2221 taatacaatc aggtagtcca accgcttcaa aagatgcacc agttactcta agtcgtggat
     2281 atgtttgttt aatatgtgct tgaatctgtc taatttgttg aatgtgacca acatggtact
     2341 gtggcatact tttcggcaaa cgattgacaa ttgtaaattc aggatcgcct ttaaatgtca
     2401 tcatttgact taaatctcta cgtacaatcg atactaattc attatctgta tgatcatcaa
     2461 ccacagtatc acctggttta cctacatacg cacgaatcaa aaccttacct tctggtgtag
     2521 taaatggcca ttttttcgat gtccaagtac atgcggtaat gtctgtatca ctcgttctcg
     2581 caatcacgaa gccagtacca tcataagtat tttcaatatc tttttcatca aatgccaata
     2641 caacagttgc aacagtcgta ctatccatcg ttttaaagta atcaaatgct ggatcttgtc
     2701 cgaaccaatt caaaaagact tgatgcggtg ttgtcactaa taccccatcg aatacatctt
     2761 cttgttgatt actgtaaaca attttatatt gcttttgaga tgtaataata tcatccactg
     2821 acgtattgta gcgtattgtc acacctttat ttttcacatc ttgttctaat gcttcaataa
     2881 atgaacttaa accatgctta aattgtttga attgtccttt tggtgcgcca ggatataatt
     2941 gtctttgttt cagacgctta tttttctcat ccttcatacc ttttatcaga cttccgaatg
     3001 cctcttcttt ttctttaaaa ttaggaaacg tactcatcaa acttaattta tcaatatcgg
     3061 taccataaat accacccatt aaaggctcta ttaaattctc aagtacctca ttacctaatc
     3121 ttgctctgaa aaatgcacca acagaaatgt caccatcttg catttgtata ggctttttga
     3181 ttaaatctaa tcctgctctt aatttaccaa gtggtgaaat taatttagtt gtcacaaacg
     3241 gtttgatatc tgtcggaatt cccataattg atccacctgg aataggatac aatttgtttt
     3301 tcgcaaaaat atatgattgt ccagtcgtat ttgtaacaat atcttgttct aatccaatat
     3361 ctttcgctaa ttctgtcata atcgtttttc tacctaaata agattcaggc cctagttcaa
     3421 tcatataacc atctttacga tacgattgaa tctttccccc cggacgattc gatgcttcaa
     3481 agatggttac atcaatatta ggatcttgct gttttaaaaa atatgcactt gataaacctg
     3541 ttatccccgc tcctataata gccactgatt tagtcacaac gtttcacgct tctttcgtca
     3601 ttaaaatata gacttgattt catcaacaat tgcaccgata aataatggat gtgtattcgg
     3661 catttttgga cgataataat tcgcaccaat atcatcgcaa actactttac attcataatc
     3721 attgtcataa agcacctcta aatgctcaca tacaaaacct actggcgtat atataaagtt
     3781 tttatactga tgtttttcat ataaatcacg tgttaaatct tgtacatctg gccctaacca
     3841 aggtgtacct gtattacctt cagattgcca accaatcgcg atatgttcaa tattagattg
     3901 ttctttaatt aaaagcgcag tatgttctag ttcttgtgga tatggatcat tattcttttc
     3961 gattaaacct tttggcaaac tatgtgccga aacaactaat accgtgtctt tatgttcctc
     4021 ttccggtatt tgagctaatg tttcgttgac tttattcgtc caatattcaa taaatttagg
     4081 ttgttcataa taatgtttca catgtgtaag ttgaatacca tattttgcag cttcttcatc
     4141 agcacgtttg tcatatgatc ctactgaaaa tgaagaataa tgtggtgcta gtactaccgt
     4201 gattgcttca gtaatgccat cattgtgcat ttgttcaacc gcatcttcga taaatggtga
     4261 aatgtgtttt aatcctaagt atagtttaaa ttcaacatct gcatatgctt tatttaatgc
     4321 tgaaactagc gcatcagcct ggtcatctgt tgtacctgct aatggtgata aaccacctat
     4381 aaattcatat ctatctttca aatcttgaag ttcttcttca gatggacgtt taccatgtct
     4441 aatatctgta taatatggct ctatgtcact ttctttataa ggtgtgccat aagccataac
     4501 taataatccc atttttttag tcattgataa taccttcctt taaatgaatt atctttcatg
     4561 tgcttcaatg taaaactatg attatctttg tgtatatgtg tgtacgaatt cgcttacttt
     4621 acgtaacgtc tctggttgca cttctgggaa tacaccgtgt cctaaattaa agatgtgttt
     4681 accgttctcc ataccttgat ctaatattgg tttcaatctc tcttcaatga cattccatgg
     4741 tgctaataaa attgatggat ctaaattccc ttgtaatgtt ttagtaacgc ctaattgttg
     4801 agcctgatta atagacgttc tccaatctag gcctaataca tcaatcggta aatcattcca
     4861 ttcattgatt aaatgactgg cacctacacc gaataaaatt accggcacat catgtttttc
     4921 tttaacctca ctgattaatc gaatcatatg tggtttaatg taacgtctgt aatcctcgac
     4981 atttaatgca cctacccatg aatcgaaaat ttgaatcaat tcggcacctg cttcgacttg
     5041 agctgttaca tatttaacag atacatcaac taaatgattc attaaagcaa accatgttgc
     5101 ttcatctcta tacatcatcg cttttgtaaa attgtaattt ttcgatggtc cgccttcaat
     5161 catatatgac gctaatgtaa atggtgcccc agtaaatcct attagcggca catttaactt
     5221 ttcttctgtt aaaagtttaa ttgtatctaa tacatatggt acatctcgtt cggggtctat
     5281 ttgagaaagt ttctcaacat cttgaattgt tttgattgga ttatgaatca ctggaccaat
     5341 acccgattta atttctacat cgacaccaat tggctttaat ggtgtcataa tatctttgta
     5401 taaaattgct gcatctgtat gataattatc aactggtaaa tgtgttacat aagcgcacaa
     5461 ctccggctga tgtgtaatat cgaatagtga atatttttct ttcaattttc gatattctgg
     5521 ttgcgaacgg ccagcttgtc gcataaacca aacaggtgta tgtgatgttt cttcaccttt
     5581 gatcattttt aaaattgtat tgtttttatt atgcaccata aaggcctcct aaattaaaat
     5641 cattcttatc tatattatca tatcgctcat tcgttcgtat tttcaataaa taaatgtc
//
LOCUS       DAMZKC010000150         5509 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.152, whole genome shotgun sequence.
ACCESSION   DAMZKC010000150 DAMZKC010000000
VERSION     DAMZKC010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5509
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.152"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            109..1251
                     /gene="bshA"
                     /locus_tag="POB55_002158"
     CDS             109..1251
                     /gene="bshA"
                     /locus_tag="POB55_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HDI6501105.1"
                     /translation="MKIGITCYPSMGGSGIIATELGIKLAERGHEVHFITSNIPFRIR
                     KPLPNMIFHQVEVNQYAVFQYPPYDITLSTKIAEVIKEYGLDLLHMHYAVPHAICGIL
                     AREMSGKDIKIMTTLHGTDITVLGYDHSLQGAIKFGIEKSDIVTSVSKSLAQETHEII
                     ETNKEIIPIYNFVRENEFPTKHNTALKSQFGIAPDEKVLIHVSNFRQVKRIDTIIETF
                     AKVREKIPSKLILLGDGPELVPMRQLTKELNVEEDVLFLGKQDCVSEFYQLSDLVLLL
                     SEKESFGLTLLEAMKTGVVPIGSNAGGIKEVIKHGETGFVVDVGDCDSASDYAIRLLE
                     DKVLYNKLQKNMLADIAERFGSELITDQYEYYYQKMLNEHNKSKGE"
     gene            1256..2458
                     /locus_tag="POB55_002159"
     CDS             1256..2458
                     /locus_tag="POB55_002159"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HDI6501106.1"
                     /translation="MDKSLFEQARPILEQIQDNGFEAYYVGGSVRDYVMGRNIHDIDI
                     TTSATPDEIESIFSHTIPVGKEHGTINVVFNDENYEVTTFRAEEDYVDHRRPSGVTFV
                     RDLYEDLQRRDFTMNAIAMDTAYKLYDYFDGQQDINNRIIRTVGIAEERFQEDALRMI
                     RCLRFQSQLSFDIATETFEAMRIQMADIKFLSIERIVIELTKLMRGINVEKSFNHLKS
                     LKAFNYMPYFEHLDMNQINVTEAIDLELLIAIVSVKFDINYSLKPLKLSNRQVKDINQ
                     YIQIMNALPSIITKEQLKMFVYDYDTHLIKNVMVAADVIKANDIQGHEPLIVNLQTID
                     ETLHRLPMHNRKDMMVNGGVLMAHLNAKSGPWLKDVLRQIEIAIVTGKVSNEETEILK
                     WVDNHVKI"
     gene            2445..3416
                     /locus_tag="POB55_002160"
     CDS             2445..3416
                     /locus_tag="POB55_002160"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HDI6501107.1"
                     /translation="MSKYSQDVLQLLYKNKPNYISGQSIAESLNISRTAVKKVIDQLK
                     LEGCKIDSVNHKGHLLQQLPDIWYQGIIDQYTKSSALFDFSEVYDSIDSTQLAAKKSL
                     VGNQSSFFILSDEQTKGRGRFNRHWSSSKGQGLWMSVVLRPNVAFSMISKFNLFIALG
                     IRDAIQHFSQDEVKVKWPNDIYIDNGKVCGFLTEMVANNDGIEAIICGIGINLTQQLE
                     NFDESIRHRATSVQLHDKNKLDRYQFLERLLQEIEKRYNQFLTLPFSEIREEYIAASN
                     IWNRTLLFTENDKQFKGQAIDLDYDGYLIVRDEAGESHRLISADIDF"
     gene            3440..>5509
                     /locus_tag="POB55_002161"
     CDS             3440..>5509
                     /locus_tag="POB55_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDI6501108.1"
                     /translation="MGMATYAVVDLETTGNQLDFDDIIQIGITFVRNNQIIDTYHSMI
                     RTNLEIPPFIQALTSIEENMLQQAPYFNQVAQEIYDKIKDCIFVAHNVDFDLNFIKKA
                     FKDCNIQYRPKKVIDTLEIFKIAFPTDKSYQLSELAEAHGITLANAHRADEDAATTAK
                     LMILAFEKFEKLPLDTLKQLYYLSKQLKYDLYDIFFEMVRQYDAKPLDKSYEKFEQII
                     YRKQVDFKKPTTNYNGSLKSLYSKAVDQLGLTYRPQQLYLAETILDQLMHSEKAMIEA
                     SLGSGKSLAYLLAALMYNIETGKHVMISTNTKLLQSQLLEKDIPAMNEALNFKINALL
                     IKSKSDYISLGLISQILKDDTSNYEVNILKMQLLIWITETPSGDIQELNLKGGQKMYF
                     DQKIETYVPARHDVHYYNFIKRNAQNIQIGITNHAHLIHSDVENSIYQLFDDCIVDEA
                     HRLPDYALNQVTNELSYADIKYQLGLIGKNENEKLLKAIDQLEKQRILEKLDIAPIDI
                     FGLKASMNEIHELNEQLFSTIFTIINDSDVYDDDIHRFHNVFTFETKDILKDLHAIID
                     KLNKTLEIFNGISHKTVKSLRKQLLYLKDKFKNIEQSLKAGHTSFISIKNLSQKSTIR
                     LYVKDYAVKDVLTKQVLEKFKSLIFISGTLKFNHSFEAFKQLFNKDVHFNTFEVNTSL
                     QSAKNTSV"
BASE COUNT         2066 a          719 c         1005 g         1719 t
ORIGIN      
        1 ttagatattt gaaattataa actcaacatt actataaact taaatttaaa caattatcta
       61 cgcatcgaga ataatagaga gaaatagaaa gaaaggatgg gcaagcgaat gaagataggt
      121 ataacatgtt acccgtccat gggtggttct ggaattattg ccacagaatt aggaattaaa
      181 ttagcagagc gaggtcacga agtacatttt atcacttcaa atatcccgtt tagaataaga
      241 aaaccattac cgaatatgat ttttcatcaa gttgaagtga atcaatatgc agtatttcag
      301 tatccaccgt atgatattac gttaagtact aaaatcgctg aagttattaa agaatatggt
      361 cttgatttgt tgcatatgca ctatgctgta cctcatgcaa tttgtgggat tttagctcgt
      421 gagatgtcag gtaaagatat aaaaattatg acaacgctac acggtactga tattactgtt
      481 ttgggctatg atcattcact ccaaggtgca attaaatttg gcattgaaaa aagtgatatt
      541 gtgacaagtg tgagtaaatc tttagcacaa gaaacacatg aaattattga aactaacaaa
      601 gaaattatac cgatttataa ttttgtacgt gaaaatgagt ttccaactaa acataacaca
      661 gcattaaaat cacaatttgg tatagcacca gatgaaaaag tgttaataca tgtatcgaat
      721 tttagacaag taaaacgcat agatactatt atagaaacat ttgcaaaagt acgtgaaaag
      781 atacctagta aattaatttt acttggagat ggtcctgaat tagtgccaat gcgtcaattg
      841 acgaaagagt taaacgtaga agaggatgtt ttatttttag ggaaacaaga ttgcgtaagt
      901 gaattttacc aattatctga cttagtatta ttattaagtg aaaaagaaag ctttggacta
      961 actttacttg aggctatgaa aacaggcgtc gttccaattg gttcaaatgc cggtggaatt
     1021 aaagaggtta ttaaacatgg tgaaactgga tttgtcgtag atgtgggtga ttgtgattca
     1081 gcgagtgact atgctatccg attacttgaa gataaggtgt tatacaacaa acttcagaaa
     1141 aacatgctag cagatattgc tgaacgtttt ggatcagaac ttattacaga tcaatatgag
     1201 tattattatc aaaaaatgct aaatgagcat aacaaaagta aaggcgagta ataatatgga
     1261 taaatcatta tttgaacagg caaggcctat attagaacaa attcaagaca atggttttga
     1321 agcatattat gtaggtggct ctgtaagaga ttatgtcatg ggaagaaata ttcatgatat
     1381 agatatcaca acaagtgcaa cgccggatga aatagaatct atctttagtc atacgatacc
     1441 tgtaggtaaa gaacatggca cgataaatgt agtttttaat gatgaaaatt atgaagtgac
     1501 aacattccgg gctgaagaag attatgtcga tcaccgtaga ccaagtggtg ttacatttgt
     1561 tcgtgattta tacgaagatt tgcaacgacg agatttcacg atgaatgcga tagcaatgga
     1621 tacagcatac aaattgtatg attattttga tggtcaacaa gatattaata atcgaataat
     1681 aagaactgta ggtatagctg aggaacgatt ccaagaagat gctttacgta tgattcgatg
     1741 tttaaggttc cagtcacaat tatcatttga tattgcaacg gaaacattcg aagcgatgcg
     1801 tatacaaatg gcagatatta aatttttatc aattgagcgt atagtgattg aactaactaa
     1861 attaatgcga ggtattaatg ttgaaaagag ttttaatcat ttaaaatcgc tgaaagcatt
     1921 taattatatg ccgtatttcg aacatcttga tatgaatcaa attaatgtaa ctgaagcaat
     1981 tgatttagaa ttgttgattg ctatagtatc agttaaattt gatattaatt actcattgaa
     2041 gcctttaaag ctaagtaacc gacaagtaaa agatatcaat caatatattc aaattatgaa
     2101 tgcattacca agtattatta caaaagaaca attaaaaatg tttgtttatg attatgatac
     2161 gcatctcatt aaaaatgtaa tggttgcagc agacgtgata aaggcaaatg atattcaagg
     2221 acatgaacca ttaatcgtta accttcaaac gattgatgaa acattacatc gtttacctat
     2281 gcataataga aaagacatga tggttaatgg cggtgtactt atggcacatt taaatgccaa
     2341 aagtggtccg tggttaaaag atgtgctaag acaaattgag atagcgattg taacaggtaa
     2401 agtaagcaac gaagaaactg aaattttgaa atgggtggat aatcatgtca aaatatagtc
     2461 aagatgtact tcaattactc tataaaaata aaccgaatta tatatctgga caaagcattg
     2521 cggaatcact taatatatcg cgcactgcag taaaaaaagt gattgaccaa ttaaagttag
     2581 agggatgtaa aatagattca gtaaatcata aagggcattt attacaacag ctcccagata
     2641 tttggtatca aggtataata gaccaatata caaaaagttc tgctttgttt gattttagtg
     2701 aagtatacga ttcaatagat tctacacaac ttgctgcgaa aaagtcactt gttggaaatc
     2761 aatcttcatt ttttatcttg agtgatgaac aaacgaaagg tcgtgggcga tttaatagac
     2821 attggagttc ttcaaaaggg caaggacttt ggatgtctgt cgtgttaaga cctaacgttg
     2881 cattctcaat gatatctaaa tttaatttat ttattgcatt agggataaga gatgcgattc
     2941 aacattttag tcaagatgaa gtcaaagtga aatggccgaa tgatatatat attgataatg
     3001 gtaaagtgtg tggtttctta actgaaatgg ttgctaataa tgatggtata gaagcaataa
     3061 tatgtggtat aggtattaat ttgacgcaac aactagaaaa ctttgatgaa agtattagac
     3121 atagagcaac aagtgtacaa ttacatgata aaaataaatt agatagatat caatttttag
     3181 agagattact tcaagaaatt gaaaaaagat ataatcaatt tttaacgtta cctttttctg
     3241 aaattcgtga agaatatatt gcagcttcta atatttggaa tagaacgttg ctatttacag
     3301 aaaatgataa acagtttaaa ggacaagcaa ttgatttaga ttacgatggc tatctaattg
     3361 ttagagatga agcgggtgaa tcacaccgtt taattagtgc agatatagat ttttaacact
     3421 aaagcaagga gagatagcta tgggtatggc aacctatgcc gttgtggatt tggaaacaac
     3481 aggcaaccaa ttagattttg acgatatcat tcaaattggt ataacgtttg tgcgcaataa
     3541 ccaaattatt gatacatatc attctatgat tagaacaaat ttagagattc cgccgttcat
     3601 tcaagcgtta acatccattg aagaaaatat gctacaacaa gcgccatatt ttaatcaagt
     3661 tgcacaagaa atctacgata agataaaaga ttgcattttt gtagctcata atgtagattt
     3721 tgatttgaat tttattaaaa aggcatttaa agattgtaat attcaatatc gtcccaaaaa
     3781 agttatagat acactagaaa tctttaaaat agcttttcct acagataaaa gctatcaatt
     3841 aagtgaatta gcagaggcac atggtattac cttagcaaat gcccatcgag ctgacgaaga
     3901 tgccgctact actgctaaat tgatgatatt agcttttgaa aaatttgaaa agttaccgtt
     3961 agatactttg aagcaattat attatttaag taaacagttg aaatacgatt tgtatgatat
     4021 tttctttgag atggttaggc aatatgatgc aaagccattg gataaatcgt acgaaaagtt
     4081 cgaacagatt atctatcgta agcaagttga ttttaaaaag ccgacaacaa attataatgg
     4141 cagcttgaag tctttatata gcaaagctgt agatcaactt ggcttaacat atcgaccgca
     4201 acaattatat ttagcggaaa ctatattaga tcagctcatg catagtgaaa aagcaatgat
     4261 tgaagcatca ctaggcagtg gtaaatcatt agcatattta cttgcggcct tgatgtataa
     4321 tattgaaact gggaagcatg tcatgatttc taccaatact aaattactgc aaagtcaatt
     4381 gttagaaaaa gatattccag caatgaatga agcgttgaat tttaaaatta atgcactatt
     4441 gattaaaagt aaaagtgatt acatttcatt ggggcttatt agtcaaattt taaaagatga
     4501 cacaagtaat tacgaagtga atatcttgaa aatgcaattg ctgatttgga ttacagagac
     4561 gccgtccgga gatatacagg aattaaattt aaaaggtggt caaaagatgt attttgacca
     4621 gaaaattgaa acatatgtac cggcacgtca cgatgttcat tattataatt ttattaagcg
     4681 aaatgcacag aacattcaaa ttggtattac aaatcatgca catttaattc attctgatgt
     4741 tgaaaattca atatatcaat tgtttgatga ttgtatagtg gatgaagcac atcgcttacc
     4801 agactatgcc ttaaatcaag tgacaaatga gttgagttat gctgatatta aatatcagct
     4861 tggtttgatt ggtaaaaacg aaaatgaaaa attgttaaag gcaattgatc aactggaaaa
     4921 gcagcgaatt ttggaaaaat tagatattgc accaattgat atttttggct tgaaggctag
     4981 tatgaatgag attcatgagt taaatgagca attgttctca acgatattta cgattatcaa
     5041 tgattctgat gtttatgatg atgatattca tcgcttccat aatgtattta cttttgaaac
     5101 aaaagacatt ttgaaggatt tacatgcgat tatcgataag ttaaataaaa cgctagaaat
     5161 attcaatggc attagtcata aaacagttaa atcattacgg aaacagttgt tgtatttaaa
     5221 agataaattt aaaaatatcg aacaaagttt aaaagctgga catactagtt tcatttcgat
     5281 taaaaattta tctcaaaaat caacaatacg tttatatgtg aaagattacg ctgtaaaaga
     5341 tgtattaacg aaacaagtat tggaaaaatt taaatcactc atttttatat ctggtacttt
     5401 aaaatttaat cattcgtttg aagcttttaa acagttattc aacaaagatg ttcactttaa
     5461 tacatttgaa gttaacacgt cgttacaaag tgcaaaaaat acaagtgta
//
LOCUS       DAMZKC010000151         5480 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.153, whole genome shotgun sequence.
ACCESSION   DAMZKC010000151 DAMZKC010000000
VERSION     DAMZKC010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5480
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.153"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            80..640
                     /locus_tag="POB55_002162"
     CDS             80..640
                     /locus_tag="POB55_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDI6501109.1"
                     /translation="MNKVEAIKFNDDIVKMYEALKIKSERDYLFFKLAIHSGLKVSEL
                     LTITVSQVKRLIEKCTLSEMCKAHFHSLIKIRLPETLSKELLQYIEDRSLSNEDVLFQ
                     SLRTNQVLSRQQAYRIIHQASIEAGIDNVGLTTLRKTFAYHAYQKGIPIPVIQKYLGH
                     QSAIETLNFIGLENECEHSIYISLQL"
     gene            659..3061
                     /gene="mnhA2"
                     /locus_tag="POB55_002163"
     CDS             659..3061
                     /gene="mnhA2"
                     /locus_tag="POB55_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit A"
                     /protein_id="HDI6501110.1"
                     /translation="MSLVYLLIAILVIMAMILLMSKRRALAKYAGYIALVAPVISSIY
                     FLIQIPSVAKLQYLSTSIPWIKTLDINLDLRLDGLSLMFSLIISLIGIAVFFYATQYL
                     SSRKDNLPRFYFYLTLFMFSMIGIVLSDNTILMYIFWELTSVSSFLLISYWYNNGDSQ
                     FGAMQSFMITVFGGLALLVGFIMLYIMTGTNNITEILGQADHIKNHGLFIPMIFMFLL
                     GAFTKSAQFPFHIWLPRAMAAPTPVSAYLHSATMVKAGIFLLLRFTPLLGLSNMYVYI
                     VTFVGLITMLFGSITALKQWDLKGILAYSTISQLGMIMAMVGIGGGYAQHQQDAIASI
                     YVFVLFGALFHLMNHAIFKCALFMGVGILDHEAGSRDIRILSGMRQLFPKMNLVMTIA
                     ALSMAGVPFLNGFLSKEMFLDALTQTGQLSQFSLISMIAIVFVGVIASVFTFTYALYM
                     VKEVFWTKYDSKVFTKKNIHEPWLFSLPSLILMVLVPVIFFVPNIFGKGIIVLALRAV
                     SGGNHQIDQLAPHVSQWHGFNIPLLLTIIIILLGSVLAIKVDWKKVFTGKIRQISVSK
                     SYEMVYRHFEKFATKRFKRVMQDRLNQYIIMTLGIFMIIIGYGYIRIGLPKVHQLHVS
                     EFGALEIILAIVTVTIGISLIFIRQRLTMVILNGVIGFVVTLFFIAMKAPDLALTQLV
                     VETITTILFIVSFSRLPNVPRSNANKKREIIKISVSLLMALIVVSLIFITQQTDGLSS
                     ISDFYLKADKLTGGKNIVNAILGDFRALDTLFEGLVLIITGLGIYTLLNYQDRRGQDE
                     RE"
     gene            3048..3473
                     /gene="mnhB2"
                     /locus_tag="POB55_002164"
     CDS             3048..3473
                     /gene="mnhB2"
                     /locus_tag="POB55_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit B"
                     /protein_id="HDI6501111.1"
                     /translation="MKENDVVLRTVTKLVVFILLTFGFYVFFAGHNNPGGGFIGGLIF
                     SSAFILMFLAFNVEEVLESLPIDFRILMIIGALVSSITAIIPMFFGKPFLSQYETTWI
                     LPILGQIHVSTITLFELGILFSVVGVIVTVMLSLSGGRS"
     gene            3470..3814
                     /gene="mnhC2"
                     /locus_tag="POB55_002165"
     CDS             3470..3814
                     /gene="mnhC2"
                     /locus_tag="POB55_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit C"
                     /protein_id="HDI6501112.1"
                     /translation="MNLILLLVIGFLVFIGTYMILSINLIRIVIGISIYTHAGNLIIM
                     SMGTYGSSRSEPLITGGNQLFVDPLLQAIVLTAIVIGFGMTAFLLVLVYRTYKVTKED
                     EIEGLRGEDDAK"
     gene            3804..5300
                     /gene="mnhD2"
                     /locus_tag="POB55_002166"
     CDS             3804..5300
                     /gene="mnhD2"
                     /locus_tag="POB55_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit D"
                     /protein_id="HDI6501113.1"
                     /translation="MLSNLLILPMLLPFLCALILVFLKNNDRISKYLYLGTMTITTII
                     SLMLLIYVQRHRPITLDFGGWSAPFGIQFLGDSLSLIMVTTASFVITLIMAYGFGRGE
                     HKANRYHLPSFILFLSVGVIGSFLTSDLFNLYVMFEIMLLASFVLITLGQSVEQLRAA
                     IIYVVLNIIGSWLFLLGIGLLYKTVGTLNFSHIAMRLNDMGDNRTVTMISLIFLVAFS
                     AKAALVLFMWLPKAYAVLNTELAALFAALMTKVGAYALIRFFTLLFDQHNDLIHPLLA
                     TMAAITMVIGAIGVIAYKDIKKIAAYQVIISIGFIILGLGTNTFAGINGAIFYLVNDI
                     VVKTLLFFIIGSLVYITGYRQYQYLNGLAKKEPLFGVAFIIMIFAIGGVPPFSGFPGK
                     VLIFQGALQNGNYIGLALMIITSLIAMYSLFRILFYMYFGDKDGEEVNFKKIPLYRKR
                     ILSILVVVVIAIGIAAPVVLNVTSDATELNTSDQLYQKLVNPHLKGED"
     gene            5301..>5480
                     /gene="mnhE2"
                     /locus_tag="POB55_002167"
     CDS             5301..>5480
                     /gene="mnhE2"
                     /locus_tag="POB55_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit E"
                     /protein_id="HDI6501114.1"
                     /translation="MNQIVLNIIIAFLWVLFQDEDHFKFSTFFSGYLIGLIVIYILHR
                     FFSDDFYVRKIWVAIK"
BASE COUNT         1722 a          758 c          987 g         2013 t
ORIGIN      
        1 taaaaaaggc aatagatata tgtaactaaa atataaaata gctatgatta agacacttaa
       61 agcaagggga ggttgagtaa tgaataaagt agaagcgatt aaatttaatg atgatattgt
      121 taaaatgtat gaagcgctca agataaaatc tgaacgtgac tatttattct ttaagttagc
      181 tatacatagt ggattgaaag tatcagaatt attaacaatt acagtctctc aagttaagag
      241 actaattgaa aagtgtacgt tatcagaaat gtgtaaagca cattttcatt cgttgattaa
      301 aattaggtta ccagaaacat tatcgaaaga actacttcaa tatatagagg acaggagtct
      361 ttcgaatgaa gacgttcttt ttcaatcact acgaacaaat caagtattat ctagacagca
      421 agcatatcga ataattcacc aagcatcaat tgaagctggt atagataatg taggactaac
      481 gacattgcgt aagacatttg catatcatgc ttatcaaaaa ggtataccta taccagtcat
      541 tcaaaagtat ttagggcatc aatctgctat tgaaacacta aattttatcg gtttagaaaa
      601 tgagtgtgaa catagtattt atatttcatt acaattatag aaacaaagga ggctaataat
      661 gagtttggtt tatttattaa ttgctatact tgtgattatg gcgatgatac ttctaatgtc
      721 taaacgtaga gcattggcta aatatgccgg gtacatagcg ttggttgcac ctgtaatttc
      781 atctatctat tttttgattc aaataccatc agtagctaaa ctgcaatatc tttctacctc
      841 tattccatgg attaagacat tagatattaa tttagattta cgtttagatg gtttaagttt
      901 aatgttttct cttattattt cacttattgg aattgcagta ttcttctatg caactcaata
      961 tttatcctct cgaaaagaca atttaccaag gttttatttt tatttaacgt tatttatgtt
     1021 cagtatgatt ggtattgtat tatcagacaa tacgatattg atgtacattt tttgggaatt
     1081 aacgagtgta tcatcatttt tattgatttc atattggtat aacaacggag atagtcaatt
     1141 tggtgcgatg caatcattta tgattacagt atttggtggt ttggcattat tagttggttt
     1201 tatcatgttg tatattatga caggaacgaa taacatcaca gagatattag gacaagcaga
     1261 tcatattaag aatcatggat tgtttatccc tatgattttt atgtttttat taggtgcatt
     1321 tacaaaatca gcacaatttc catttcatat ttggctacct agagcaatgg ctgcacctac
     1381 acctgtaagt gcttatttac attcagccac gatggtaaaa gctggtatct ttttattact
     1441 tcgatttaca ccattattag gtcttagcaa tatgtacgta tatatcgtta cgtttgttgg
     1501 tttaataaca atgttatttg gttcaattac agctttaaaa caatgggatt taaaaggtat
     1561 cctagcgtac tctacaatca gtcaacttgg gatgattatg gctatggtgg gtataggtgg
     1621 cggatatgct caacaccaac aagacgcaat agcatctatt tatgtatttg tattatttgg
     1681 tgcgctattt catctaatga atcatgccat ctttaaatgt gcgcttttca tgggagtagg
     1741 tattttagat catgaagcag gttcaaggga tatacgaatt ttaagtggaa tgcgtcaact
     1801 atttcctaaa atgaatctag tcatgacgat agcggctcta tctatggctg gagtaccatt
     1861 tttaaatgga tttttaagta aagaaatgtt tttagatgca ttaacacaaa ctggacaatt
     1921 atcccaattt agtttgattt caatgatagc tatcgtgttt gttggtgtta ttgcgagtgt
     1981 ttttacattc acatatgcac tatacatggt aaaagaagta ttttggacaa aatatgattc
     2041 taaggttttt actaaaaaaa atatccacga accatggttg tttagtttac catctcttat
     2101 attaatggtg ctagtacctg taatcttttt tgtaccaaat atatttggga aggggattat
     2161 cgttctagca ttaagagctg tatcaggtgg taatcatcaa attgatcaat tggcaccaca
     2221 tgtttcgcaa tggcatggat ttaacatacc gcttctttta accatcatca ttattttatt
     2281 gggtagtgta ctagcaatca aagtagattg gaaaaaagtg ttcacaggta aaattagaca
     2341 gatttcagtt tcaaaaagct atgagatggt atatcgacat tttgaaaagt ttgctacgaa
     2401 gcgatttaaa cgtgttatgc aagatcgttt aaaccaatac attattatga ccttaggcat
     2461 atttatgatt atcattggat atggttatat tcgaattgga cttcctaaag tacatcagtt
     2521 acatgtttct gaatttgggg cattagaaat tatattagca atcgtaactg tcacaattgg
     2581 tatttcttta atttttatac gtcaacgact gacaatggtc attttaaatg gagtcatcgg
     2641 atttgttgtg accttattct ttatagcaat gaaagcccct gatctagcat tgactcagct
     2701 agtagttgaa acaataacga cgatactatt tattgtcagt ttttcaagat taccaaacgt
     2761 gccaagatct aacgctaaca aaaaaagaga aataattaaa atttctgtat cactcttgat
     2821 ggcacttatt gttgtatcat taatttttat tacacaacaa acagatggtt tatcatcaat
     2881 atcagacttt tatttaaaag ctgacaaact aacaggtggt aaaaatattg taaatgcgat
     2941 acttggtgac tttagagcat tagatacatt atttgaagga ttagtgttaa ttattactgg
     3001 gctaggtatt tacacattat taaattatca agatcggagg ggacaagatg aaagagaatg
     3061 atgtcgtgtt aagaacggtc acgaaacttg ttgtatttat tttattgact ttcggattct
     3121 atgtcttctt cgcaggtcat aataatcctg gtggtgggtt tattggtggt ttaatattta
     3181 gttcagcgtt tattttaatg tttctggctt ttaatgttga agaggtttta gaaagtttac
     3241 cgattgattt tagaatttta atgattattg gagcattggt atcatctatt actgcgataa
     3301 tacctatgtt ttttggaaaa ccatttttgt ctcaatatga aacaacttgg atacttccaa
     3361 ttttaggaca aattcatgta agtacaataa cactttttga attaggtatt ttattctcag
     3421 ttgttggtgt tattgtcaca gtgatgttgt cgcttagcgg aggtcgatca tgaatttaat
     3481 attattacta gttataggat ttttagtgtt tataggaaca tatatgattt tatcaatcaa
     3541 tttaattcgt attgtaatcg gaatttcaat atatactcat gctggtaatc tcattattat
     3601 gagtatggga acgtatggtt ctagtagatc agaaccacta ataactggtg gaaaccaatt
     3661 gtttgttgat cccttgttac aagctattgt actaactgca atagttatag ggtttgggat
     3721 gactgcgttt ttacttgtac ttgtttatag aacttataaa gtaacaaaag aagatgaaat
     3781 tgaaggccta aggggggaag atgatgctaa gtaacttatt gattttacca atgttattac
     3841 cattcctttg tgccttaatc cttgtatttt taaaaaataa tgatcgtatt tctaaatatt
     3901 tatacttagg tacaatgact atcaccacaa ttatttcatt aatgctatta atttatgttc
     3961 agcgtcaccg tccaattacg ctagactttg gaggatggtc agcgcccttt ggtatacagt
     4021 ttttaggaga ttctttaagt ttaattatgg ttacaaccgc ttcgtttgtg attactttaa
     4081 ttatggcata cggatttggg cgtggcgaac ataaagcaaa tcgttatcac ttgccatcgt
     4141 tcatattatt tttaagtgtt ggcgtgatag gctcttttct aacatcagat ttatttaatt
     4201 tatacgtcat gtttgaaatt atgttactag cgtcatttgt actcattaca cttggacaat
     4261 ctgtagaaca attacgtgct gcaattattt atgttgtctt gaatattatt ggttcatggc
     4321 tattcttatt aggtataggt ttactttata aaacagtagg tacattaaac ttttcacata
     4381 ttgcaatgcg tttgaatgac atgggagata atcgcactgt tacaatgatt tcattaatct
     4441 tcttagtcgc atttagtgcg aaagcagcgc tggtcctttt tatgtggcta cccaaagcct
     4501 acgctgtgtt aaatactgag cttgcagcat tatttgcagc gttaatgacc aaagtagggg
     4561 cctatgcatt aattcgattc ttcactttac tatttgatca acataatgat ctcatacatc
     4621 cattgctagc aactatggct gctataacta tggtcatcgg cgctataggt gtcattgctt
     4681 ataaagatat taaaaagatt gcagcttacc aagtcataat ctcaatagga tttatcattt
     4741 taggtttagg aacaaacacg tttgcaggta ttaatggtgc aatattttat ttggtaaatg
     4801 acattgttgt aaaaacattg ctatttttta ttattggtag tttagtttac attacaggct
     4861 atcgacaata tcaatatttg aatggcttag ctaaaaaaga acctttattt ggagttgcgt
     4921 ttattataat gatttttgct attggcggcg tgcctccatt tagtggcttt ccggggaaag
     4981 tacttatttt ccaaggtgca ttgcaaaatg gcaattatat tggactagcg ttaatgatta
     5041 ttactagtct aattgcaatg tacagtttat ttaggatact tttttatatg tattttggag
     5101 ataaagatgg ggaggaagtt aattttaaga aaatcccgct atatcgaaaa agaattttaa
     5161 gtattttagt agttgtggtt atcgcaatcg gaattgctgc acctgttgtg ttaaatgtta
     5221 caagtgatgc aactgagttg aacacgagtg atcaattata tcaaaaactt gtaaatccgc
     5281 atttgaaagg agaggactaa atgaatcaaa tagttttaaa tattatcatt gcattcttat
     5341 gggtattatt tcaagatgaa gatcatttta aattctcgac tttcttttct ggatatctaa
     5401 ttggtttaat tgtcatttat atattacaca ggtttttcag cgatgatttt tatgttagaa
     5461 aaatatgggt agctattaaa
//
LOCUS       DAMZKC010000152         5438 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.154, whole genome shotgun sequence.
ACCESSION   DAMZKC010000152 DAMZKC010000000
VERSION     DAMZKC010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5438
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.154"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(54..1772)
                     /gene="ptsP"
                     /locus_tag="POB55_002168"
     CDS             complement(54..1772)
                     /gene="ptsP"
                     /locus_tag="POB55_002168"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDI6501115.1"
                     /translation="MSKLIKGIAASDGVAIAKAYLLVEPDLTFDKNEKVTDVEGEVAK
                     FNSAIEASKVELTKIRNNAEVQLGADKAAIFDAHLLVLDDPELIQPIQDKIKNENANA
                     ATALTDVTTQFVTIFESMDNEYMKERAADIRDVSKRVLSHILGVELPNPSMIDESVVI
                     VGNDLTPSDTAQLNKEFVQGFATNIGGRTSHSAIMSRSLEIPAIVGTKSITQEVKQGD
                     MIIVDGLNGDVIVNPTEDELIAYQDKRERYFADKKELQKLRDADTVTVDGVHAELAAN
                     IGTPNDLPGVIENGAQGIGLYRTEFLYMGRDQMPTEEEQFEAYKEVLEAMDGKRVVVR
                     TLDIGGDKELSYLNLPEEMNPFLGYRAIRLCLAQQDIFRPQLRALLRASVYGKLNIMF
                     PMVATINEFREAKAILLEEKENLKNEGHDISDDIELGIMVEIPATAALADVFAKEVDF
                     FSIGTNDLIQYTLAADRMSERVSYLYQPYNPSILRLVKQVIEASHKEGKWTGMCGEMA
                     GDETAIPLLLGLGLDEFSMSATSILKARRQINGLSKNEMTELANRAVDCATQEEVIEL
                     VNNYVK"
     gene            complement(1775..2041)
                     /locus_tag="POB55_002169"
     CDS             complement(1775..2041)
                     /locus_tag="POB55_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HDI6501116.1"
                     /translation="MEQNSYVIIDETGIHARPATMLVQTASKFDSDIQLEYNGKKVNL
                     KSIMGVMSLGVGKDAEITIYADGSDESDAIQAISDVLSKEGLTK"
     gene            complement(2195..2737)
                     /locus_tag="POB55_002170"
     CDS             complement(2195..2737)
                     /locus_tag="POB55_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF697 domain-containing protein"
                     /protein_id="HDI6501117.1"
                     /translation="MGFKNNLTSNLTNKIGNSVFKIENVDGKGAMPTTIQELRERRQR
                     AEAIVKRKSLMSSTMSVVPIPGLDFGVDLKLMKDIIEDVNKIYGLDHKQVNSLGDDVK
                     ERIMSAAAIQGSQFIGKRISSAFLKIVIRDVAKRTAAKQTKWFPVVGQAVSASISYYF
                     MNKIGKDHIQKCENVIKNVM"
     gene            complement(2791..3963)
                     /locus_tag="POB55_002171"
     CDS             complement(2791..3963)
                     /locus_tag="POB55_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HDI6501118.1"
                     /translation="MKIATLNKGKETKYFNGYPLIEEEDIYSQDHLKEGDIFQIVTDK
                     SQYVATAYVGRQHKGLGWVLTYDKAQEINTAFFVKLFNTALAERDYYFNIDGTNAFRL
                     FNAEGDGVGGLTIDNYDGHLLIQWYSKGIYKFKYAILEAVRKVFDYKSIYEKVRFKDS
                     EYSGGFVEGDAPEFPIVIEENFTFYNVDLEDGLMTGIFLDQKEVRKKLRDQYAKERHV
                     LNLFSYTGAFSVIAASEASSTTSVDLANRSRSLTEENFGLNAIDPKSQYIYVMDTFDF
                     YKYAARHGHSYDTIVIDPPSFARNKKRTFSVQKDYDKLINGALNILSSEGTLLLCTNA
                     SVYPLKQFKNTIKKTLEESGVDYELTEVMGLPKDFKTHPHYKPSKYLKAVFVNIRH"
     gene            complement(4388..>5438)
                     /locus_tag="POB55_002172"
     CDS             complement(4388..>5438)
                     /locus_tag="POB55_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501119.1"
                     /translation="FGILVMLIGHTLLRTIKYDYDISIFFLVIGIMYFIITLILMTQV
                     YGFFAVIFIIPFTIHIGYIVYKDELNQENVKNHFMWIIVSYGISYLITQIALYGRIDA
                     NEIESIDILSVNAFFIIMWLLGQMAIWNFLFLRRALPLTKQELGEEEPELSRTSKGNV
                     TNQTKIHLKQLQDKTTEYARKTRRSVDLDKIRAKRDKFKKKVNDIIDIQEDDIPDWMR
                     KPKWVKPMYVELFCGVVIFLFTFLEFNNRNALFVSGDWKLSQTQYVIEWVTLLILLFI
                     IIAYIATTLTFHLKGKFYYLQLFMGSILFFKLLTEFINIMIHGLLLSVFITPTLLLML
                     LAIIISYSLQLRERP"
BASE COUNT         1750 a          999 c          716 g         1973 t
ORIGIN      
        1 ctcacaatga atacttcggg tttatttata attctgtctt tcgaaagaat taattatttt
       61 acgtagttgt taactaattc aataacttct tcttgcgttg cacagtcgac tgcacggtta
      121 gcaagttcag tcatttcatt tttacttaaa ccattaattt gtcttcttgc tttcagaata
      181 gacgttgcac tcatagagaa ctcatctaaa cctaaaccaa gcaataatgg aatagctgtt
      241 tcatctccag ccatttcacc acacatacct gtccatttac cttctttatg tgacgcttca
      301 ataacttgtt taactaaacg taagattgaa gggttatatg gttggtatag atatgataca
      361 cgctctgaca tacggtcagc agctaatgtg tattgaatta aatcatttgt accgatactg
      421 aagaaatcta cttctttagc aaagacatca gctaatgctg ctgttgcagg tatctctacc
      481 atgattccta attctatatc atccgaaatg tcatgacctt catttttaag gttttctttt
      541 tcttctaata atatagcttt agcttctcta aattcgttaa ttgttgcaac cattgggaac
      601 atgatattta acttaccata aactgatgca cgtaataatg cacgtagctg tggtctgaaa
      661 atatcttgtt gcgcaaggca taaacgaatc gcacggtaac ctaagaatgg attcatttct
      721 tcaggcaagt ttaagtatga taattcttta tctccaccta tatctaaagt acgtacaaca
      781 acacgtttac cgtccattgc ttctaatact tctttataag cttcaaattg ttcttcttct
      841 gtaggcattt ggtcacgacc catatataaa aactcagttc tatataagcc gataccttgt
      901 gcaccatttt caataacacc tggcaaatca ttaggtgtac caatatttgc agcaagctct
      961 gcgtgaacac catcaactgt aacagtatca gcatcacgta gtttttgtaa ttctttcttg
     1021 tcagcaaaat aacgctcacg tttatcttga taagcgatta actcatcttc agttggatta
     1081 acgattacat caccatttaa tccatctacg ataatcatgt cgccttgttt aacttcttga
     1141 gtaattgatt ttgtaccaac aattgctgga atttctaaag aacgactcat aattgcagag
     1201 tgacttgttc ttccgccaat gtttgtagca aaaccttgta cgaattcttt atttaattga
     1261 gcagtatcag atggcgttaa gtcattccct acaataacaa cgctttcatc aatcatactc
     1321 ggattcggta attctacacc taaaatatgt gataacacac gtttagaaac gtcgcgaata
     1381 tcagccgcac gttctttcat gtattcgtta tccatagatt caaaaattgt aacaaattgt
     1441 gttgttacat ccgttaatgc tgtagcagca ttagcgtttt catttttaat cttatcttgg
     1501 attggttgaa ttaattcagg gtcatctaaa actaataaat gtgcatcaaa gatagcagct
     1561 ttatcagcac ctagttgaac ctctgcatta tttctaattt tagttaactc aactttagaa
     1621 gcttcgatag cgctattgaa ctttgcaact tctccttcaa catcagtgac tttttcattt
     1681 ttgtcgaatg ttaagtctgg ctcaactaat aaataagctt tagcaattgc gacaccatca
     1741 gatgcggcaa tacctttaat taatttagac atgattattt agtcaatcct tcttttgata
     1801 agacgtcact gattgcttga atggcgtcag attcatcact accgtcagca taaattgtaa
     1861 tttcagcatc tttaccaaca ccaaggctca taacacccat gattgatttt aagtttactt
     1921 tcttaccgtt atattctaat tgaatatcag aatcgaattt tgaagctgtt tgtactaaca
     1981 ttgttgctgg tctagcgtga ataccagtct cgtcgatgat tacatatgaa ttttgttcca
     2041 taattacatt tctccttcgt atagttagaa tcttcaatat tattacaata ctaacttagt
     2101 tttatacatt aactttacca aaaccatgct ttaaattcaa ttcgtttaaa accggtttca
     2161 gtaaaatttg caaattgcaa aactattata gcacctacat gacattttta ataacatttt
     2221 cgcatttttg aatgtgatct tttccaattt tattcataaa atagtaacta atagatgcag
     2281 acacagcttg tcctacaaca ggaaaccatt ttgtttgttt tgcagcagta cgtttagcta
     2341 catctctaat aactattttt aaaaatgcac ttgaaattct tttaccaata aattgactac
     2401 cttgaattgc tgctgcagac ataattcttt ctttcacatc atccccaagg ctattaactt
     2461 gcttatgatc taatccgtat attttattta catcttcgat aatatctttc attaatttta
     2521 aatcaacacc aaaatctaaa cccggaattg gaacaacgct cattgttgat gacattaaag
     2581 attttctctt tacaattgct tcagcacgtt gtcgtctttc tctcaattct tgaatcgtcg
     2641 ttggcattgc accttttccg tcaacatttt ctattttaaa gactgaatta ccaattttat
     2701 ttgttaaatt tgatgttaaa ttgtttttga atcccataat attatcaccc ttctttttct
     2761 caataagtta ctatacccct ttttatcaaa ttaatgtcta atatttacaa aaacagcttt
     2821 taaatattta gatggcttat aatgtggatg cgttttaaaa tcttttggta atcccataac
     2881 ttcagttaat tcataatcaa cgccactctc ttcaagcgtc tttttaatag tatttttaaa
     2941 ttgctttaat ggatatacac ttgcgtttgt acataacaat aatgttcctt cagatgataa
     3001 gatatttaag gcgccattaa ttaatttgtc ataatctttt tgcactgaaa atgtacgttt
     3061 tttgttacgc gcaaagctag gtggatcaat cacgatcgtg tcataactat gtccatgtcg
     3121 tgcagcatat ttatagaaat caaaagtgtc catgacataa atatattggg atttaggatc
     3181 aatagcattt aatccaaaat tttcttcagt taaactacga gaacgattag ccaaatctac
     3241 acttgttgtt gaagatgcct cacttgctgc tattacagaa aaagcacctg tataactaaa
     3301 taagtttaaa acatggcgtt ctttggcata ttgatccctt aatttcttgc gcacttcttt
     3361 ttgatctaaa aagatacctg tcatcaaacc atcttcaagg tctacattat aaaatgtgaa
     3421 gttttcttcg ataacaattg gaaactcagg tgcatctcct tcaacaaaac caccactata
     3481 ttcgctgtct ttaaatctta ctttttcgta aatagattta taatcaaata cttttctaac
     3541 cgcttcaaga atggcatatt taaatttata aatacctttt gagtaccatt gaatcaacaa
     3601 atgaccatcg taattgtcga ttgttaatcc cccaacacca tcaccttcag cattaaataa
     3661 tctaaaagca tttgttccat ctatattaaa ataataatca cgttctgcta atgcagtatt
     3721 aaacaatttc acaaagaaag ctgtgttgat ttcttgagct ttatcgtatg ttagaaccca
     3781 acctaatcct ttatgttggc gaccaacata agccgttgca acatattgtg atttatcagt
     3841 cacaatttga aaaatatctc cttcttttaa atgatcttgt gaatagatat cctcttcttc
     3901 aattaaagga tatccattaa aatattttgt ttctttgcct ttgttcagag ttgctatttt
     3961 catctgacaa cttcctttct gtgtttcgtt gtagttttat atagattaaa ttgtttgcca
     4021 aatttcaata tcatctactt ctaatcttaa tgttgatttt aacaaattag taattcgaat
     4081 gatatatgtt aaatttctac aaaggaaata ctatatatcg tttcgcaatt tccaaatgtc
     4141 gattcaacta tttaatagtt ttcttgattt tacaaatgga taacttttca actattttat
     4201 ttgcactaaa actttgtata caattttcaa tacttaattc acatcacctg aatcaatcaa
     4261 atctagttac aaaaaaagta ctatccataa acttcattga cctgtatcta aataagttag
     4321 agatatatct aacttatagc gtacacatca aaatgtttaa tagatagtac ttttataatg
     4381 cttttaatta tggtcgctct cgtaattgta acgaataaga aatgatgatt gccaataaca
     4441 ttaatagtaa tgttggtgta atgaacactg aaagtagtag tccatgaatc attatattta
     4501 tgaattccgt taacaattta aagaataaaa tgctccccat aaatagttgt aaataataaa
     4561 acttaccttt caagtggaaa gttaacgttg tagcgatata tgcgataata atgaataaca
     4621 gaattagtaa tgtaacccat tcaataacat attgtgtctg tgataatttc caatcaccag
     4681 atacaaataa tgcattacga ttattaaatt ctaaaaatgt gaataaaaag atgacgacac
     4741 cacaaaatag ttcgacatac attggtttaa cccatttcgg ttttctcatc caatcaggaa
     4801 tatcgtcttc ttgaatatcg ataatatcat taactttctt tttgaattta tctcttttag
     4861 ctctaatttt atctaaatcg acacttcttc ttgtcttacg tgcatattct gtagtcttat
     4921 cttggagttg tttcaagtga attttagttt gattcgtgac attcccttta cttgttcttg
     4981 ataattctgg ctcctcttca cctaattctt gctttgttaa aggtaaagct cggcgcaaga
     5041 acaagaaatt ccaaatagcc atttgaccaa gtaaccacat aattataaag aaagcattga
     5101 cacttaagat atcaattgac tctatttcat tagcatcaat tctgccatac aatgcaattt
     5161 gtgtaattaa gtaacttata ccataactca caattatcca catgaaatga ttttttacat
     5221 tttcctgatt caattcatct ttatagacga tatatcctat atgaattgta aatggaataa
     5281 tgaaaatcac tgcaaagaat ccataaactt gagtcattaa tataagagta ataataaagt
     5341 acatgatacc gataactaag aaaaagatag aaatatcata gtcatactta atagttctaa
     5401 gtaatgtatg accaatcaac ataactaaaa taccgaat
//
LOCUS       DAMZKC010000153         5418 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.155, whole genome shotgun sequence.
ACCESSION   DAMZKC010000153 DAMZKC010000000
VERSION     DAMZKC010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5418
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.155"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            252..638
                     /locus_tag="POB55_002173"
     CDS             252..638
                     /locus_tag="POB55_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HDI6501120.1"
                     /translation="MAIVNKVIIVEGKSDKKRVQQVIAEPVNIICTHGTMSIDKLDDM
                     IESLYDKQVFVLADSDDEGDRIRNWFKRYLSESEHIFIDKTYCQVANCPKQYLAHVLS
                     KHGFTCKKETPLLPNINNERLVLVNE"
     gene            631..927
                     /locus_tag="POB55_002174"
     CDS             631..927
                     /locus_tag="POB55_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDI6501121.1"
                     /translation="MNNSLDIKDVTTFYEEDKHLIFGYTPTCGTCKVSERMLDIANEI
                     LQLPLLKIDLNFYPQFCKDMQIMSTPILLLMNKDKEVKRIYAFKSVTDLLENLK"
     regulatory      985..1088
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            1177..2202
                     /locus_tag="POB55_002175"
     CDS             1177..2202
                     /locus_tag="POB55_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HDI6501122.1"
                     /translation="MIELKEVVKEYRTKNKEVLAVDHVNLSIRAGSIYGVIGFSGAGK
                     STLIRMFNHLEAPTSGEVIIDGDHIGQLSKNGLRAKRQKVSMIFQHFNLLWSRTVLKN
                     IMFPLEIAGVPRRRAKQKALELVELVGLKGREKAYPSELSGGQKQRVGIARALANDPT
                     VLLCDEATSALDPQTTDEILDLLLKIREQQNLTIVLITHEMHVIRRICDEVAVMESGK
                     VIEQGPVTQVFENPQHTVTKRFVKDDLNDDFETSLTELEPLEKDAYIVRLVFAGSTTT
                     EPIVSSLSTAYDIKINILEANIKNTKNGTVGFLVLHIPYISSVDFGKFEKELIERQVK
                     MEVLRHG"
     gene            2195..2890
                     /locus_tag="POB55_002176"
     CDS             2195..2890
                     /locus_tag="POB55_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6501123.1"
                     /translation="MGKSFSEIINEMITMPNIQWPEVWTAIVETLYMTVVSTIFAFIL
                     GLILGVLLFLSAKGKSIGARLFYSIVSFIVNLFRAIPFIILILLLIPFTSLILGTISG
                     PTGALPALIIGAAPFYARLVEIAFKEIDKGVIEAAWSMGANTWTVIRKVLLPEAMPAL
                     VSGITVTAIALVGSTAVAGVIGAGGLGNLAYLTGFTRNQNDVILVSTVFILIIVFIIQ
                     FIGDWLTNKLDKR"
     gene            2908..3729
                     /locus_tag="POB55_002177"
     CDS             2908..3729
                     /locus_tag="POB55_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDI6501124.1"
                     /translation="MKKLFGLILVLTFAVVLAACGNGNKSGSDDKKITVGASPAPHAE
                     ILEKAKPLLEKKGYELDIKTINDYTTPNKLLDKGEIDANYFQHTPYLNTEKKDKGYKI
                     VSAGDVHLEPMAVYSKKYKSLKELPKGATVYVSNNPAEQGRFLKFFVDAGLIKVKKGV
                     KIEDAKFSDITENKKDIKFNNKQSAEFLPKIYQNEDADAVIINSNFAIEQKLNPKKDS
                     IAVESAKDNPYANLIAVKEGHQDDKKIKALIEVLQSKDIQDFINEKYNGAVIPAK"
     gene            4083..4277
                     /locus_tag="POB55_002178"
     CDS             4083..4277
                     /locus_tag="POB55_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDI6501125.1"
                     /translation="MADESKFEQAKGNVKETVGNVTDNKNLENEGKEDKASGKAKEFV
                     ENAKEKATDFIDKVKGNKGE"
     gene            complement(4510..5361)
                     /locus_tag="POB55_002179"
     CDS             complement(4510..5361)
                     /locus_tag="POB55_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HDI6501126.1"
                     /translation="MQQFKWINILKGFAMGTSDLVPGVSGGTIALLLGIYNQFIASIS
                     GIFSRRFWPSFTFLIPIIIGMLLAMGSLSNLFNYLLSQHHIPTMFFFGGLIIGIVPYL
                     LKISNYKTSFTTKHYMMVIAGIAILIVITLMNNGDKHAGETLTLSTSLIIKYFIAGMC
                     ASSAMLLPGISGSFMLLVFGFYGTVMLAISEVVKLNFAGLPILLAVGFGVLAGFIISS
                     KIIQYFLTHHKLMTFALIIGFVVGSLFAVFPGLPTNIVMWFVSLVVFIIGFIVSLTLG
                     RITAENE"
BASE COUNT         1992 a          753 c          960 g         1713 t
ORIGIN      
        1 ataattgaaa agtttgttat aggtgtattt tcttttggtt aactattgtt aatataacat
       61 tgtagatttt aggatgttga tttttgactt agcctttgtc tgcttatgtt aaatgttcca
      121 gctagtcaaa cattgagttt gtaattatta tattaagcta gtttaaattt tttgtgattt
      181 atgttttatt cataccaatt ttgtgaaatg atacagtgac agatgtttat tgattttaaa
      241 gggtgatgat gatggctatt gtaaataaag tgataattgt tgaaggaaaa tctgataaaa
      301 aaagggtgca acaggttatt gcagaaccag tcaatattat ttgtactcat ggaacaatga
      361 gtatagataa gcttgatgat atgatagaat cactgtatga taaacaagtt tttgtattag
      421 ccgattctga tgacgaagga gatcgaatta gaaattggtt taaacgttat ttgagtgaaa
      481 gtgaacatat atttattgat aaaacttact gtcaagttgc gaattgcccc aaacaatatt
      541 tggcgcatgt actttcaaaa catggcttta cttgtaagaa agaaacacct cttttaccga
      601 atataaataa tgaaaggtta gttttagtaa atgaataatt cattagacat caaagatgta
      661 actacatttt atgaggaaga caaacattta atctttggtt atacaccaac gtgtggtact
      721 tgtaaggttt cagaaagaat gttagacatt gctaatgaaa tattgcagtt accattattg
      781 aaaatagatt taaactttta tcctcagttt tgtaaagata tgcaaatcat gtctacgccg
      841 attttattgt tgatgaataa agataaagaa gtaaaacgaa tttatgcatt taaatcggtg
      901 actgatttgt tagaaaattt aaaatagtta ttgacgaaat tttactaagg tgttaggatt
      961 aatacaaatt aaatgatgcg taaactctta tcgagagtgg tggagggatg tgccctacga
     1021 agcccggcaa ccgtcttata tagaaatggt gccaattcac ataaagtttt aacttttgaa
     1081 gatgagagaa acaatactac tattgctttc tcaatttttc tatcgatatt gagaaagcat
     1141 tttttatttt attaagcaac acagggagga atcaacgtga ttgaattaaa agaagttgtt
     1201 aaagaatatc ggactaaaaa taaagaagtc cttgctgtag atcacgttaa tttatcgatt
     1261 cgagcaggat cgatttatgg cgtcattggt ttttctggag caggaaaaag tactttgatt
     1321 cgaatgttta atcatttaga agcgcctaca tcaggtgaag ttattataga tggagaccat
     1381 ataggtcaat tgtccaaaaa tggattaaga gcaaaaagac aaaaagtaag tatgatcttc
     1441 caacatttta atttgttatg gtcaaggact gtgttaaaaa atattatgtt tccgcttgaa
     1501 attgcaggtg tccctagaag gagagctaag caaaaagcat tagaacttgt cgaactcgtc
     1561 ggtttaaaag gtagagaaaa ggcttatcca tcagagttat caggtggaca aaagcaacgt
     1621 gttgggattg cacgagcgtt agctaatgat ccaacggtct tgctttgtga tgaggcaaca
     1681 agtgcacttg atccgcaaac aacagatgaa attttagatc tactactaaa aattagagaa
     1741 caacaaaatt taacaattgt actaattacg catgaaatgc atgtcattcg tcgtatttgt
     1801 gatgaagttg cagttatgga aagtggtaaa gtgatagaac aaggaccggt gacacaggtt
     1861 tttgaaaatc cgcaacacac tgtgacaaaa cgatttgtga aagacgattt aaatgatgat
     1921 ttcgaaacat ctttaacaga attagagcca ttagaaaaag atgcatatat cgttagatta
     1981 gttttcgctg gttcaacaac aaccgagcct attgtatcga gtctatcaac tgcctatgat
     2041 attaaaatta atattttaga agcaaatatt aaaaatacaa aaaatggaac agtcggcttt
     2101 ttagttctgc atattccata tatttcaagt gtagatttcg gaaaattcga aaaagagtta
     2161 attgagcgac aagttaaaat ggaggtgtta agacatgggt aaatcattta gtgaaattat
     2221 aaatgaaatg attacaatgc ctaatattca gtggccagaa gtttggactg caatagtcga
     2281 aacactatac atgacagtcg tctcaactat atttgcattt atacttggtc ttattttagg
     2341 tgtgttatta ttcttgtctg ctaaaggtaa gtctatcggt gcaaggttat tttattctat
     2401 cgtttctttc attgttaact tatttagagc gataccattt attattttaa ttttattatt
     2461 aattccattt acaagtttga tacttggaac gataagtggt ccgacaggtg cgttaccagc
     2521 cttgatcatt ggcgcagcac cgttttatgc aaggctcgta gaaattgctt ttaaagaaat
     2581 tgataaaggt gtcatcgaag cggcttggtc aatgggcgct aatacttgga cagtaattcg
     2641 taaagtcctt ttacctgaag ctatgccagc gctagtgtct ggcattacag ttacagcaat
     2701 cgctttagtt ggttcaacag cagttgcagg tgtaattggt gccggtggtt taggaaattt
     2761 agcatactta acaggtttca ctcgaaatca aaatgatgtc attttagtat caacagtttt
     2821 tattttaatt attgtattta taatccaatt cattggggat tggcttacaa ataaacttga
     2881 taaacgataa attgggggtt tcattttatg aaaaaattat ttggtcttat tttagtatta
     2941 acatttgcag ttgtattagc agcttgcggt aatggaaaca aaagtggcag tgacgacaag
     3001 aaaataacag taggtgcttc accagcacca catgctgaaa ttttagaaaa agcaaaacca
     3061 ttattagaga aaaaaggtta tgaactagat attaaaacaa ttaacgatta cactacacct
     3121 aataaattac tagacaaagg tgaaattgac gcaaactatt tccaacatac accatattta
     3181 aacacagaga aaaaggataa aggttacaaa atcgtaagtg ccggtgatgt tcacttagaa
     3241 cctatggctg tatactctaa aaagtataaa agtttaaaag aattaccaaa aggtgcaaca
     3301 gtctatgtgt ctaataatcc agctgaacaa ggacgtttct taaaattctt cgttgatgca
     3361 ggtttaatta aagtcaaaaa aggcgtaaaa attgaagatg ctaagtttag tgatattaca
     3421 gagaataaaa aagatattaa gtttaataat aaacaatcag cagaattctt acctaaaatt
     3481 tatcaaaatg aagacgctga tgctgttatc attaattcga actttgcaat cgaacaaaaa
     3541 ctaaatccta aaaaagattc tattgctgta gaaagtgcga aagataatcc ttatgcaaac
     3601 ttaattgctg ttaaagaagg acatcaagat gataagaaaa tcaaagcatt aattgaagta
     3661 ttacaatcta aagatattca agacttcatt aatgaaaaat acaatggtgc agttattcct
     3721 gctaaataaa tcatattaat ggctcagtaa cgtgatgttg ctgggctttt taatttaaac
     3781 aggtatttat atgatattta ggaatgagat gctattacgg ataaagtaaa atcctatatg
     3841 catgcgtgct actcatttat tcttattttt aatttcacat ggctagtgac taatacatca
     3901 taaatcatta gtgattcttt attatttcag tcccactccc tcaagttaat gatgaatcta
     3961 tttcgctagt tataaaatga cgtcaaatca atatcaaaaa ttatttaagt aaaatgttta
     4021 gataattttt cagtgggtaa gtattatata taacatttaa ttattccgag gaggcattta
     4081 ttatggcaga cgaaagtaaa tttgaacaag caaaaggtaa tgttaaagaa acagtaggta
     4141 atgttactga taataaaaat ttagaaaacg aaggtaaaga agataaagct tctggtaaag
     4201 cgaaagaatt cgttgaaaat gcaaaagaaa aagcaactga ttttattgat aaagtaaaag
     4261 gtaacaaagg cgagtaattt tactttttat tcctaataca ctgcacatgc cacttagtcg
     4321 gtataaccat cggctaggtg gtttttgtat taaaaaagtg gataccaaaa tttattaata
     4381 attattttaa tgttagaaaa aaactaaata aaaactcgct aatgatatcc aataatatgt
     4441 atacaaaacg agacatatat tgcatatgat taacgagata ctgaaaatat tttatcaccc
     4501 ctaaaatgat tattcatttt cagcggtaat tcgacctaaa gtcaaactta caataaaacc
     4561 gatgataaat actactaatg aaacgaacca catcacgata ttagttggta aacctggaaa
     4621 tactgcaaag agggagccaa caacaaaacc aatgattaat gcaaaagtca ttagtttatg
     4681 atgtgttagg aaatactgga taattttgct tgaaataatg aatccagcaa gcacgccaaa
     4741 tccgactgca agtaatatag gaagacctgc aaagttaagt ttaacaactt cagatattgc
     4801 tagcatgacc gtaccataga agccaaatac taataacata aatgaccctg aaatacctgg
     4861 gagtaacata gcactagatg cacacatacc tgcaataaaa tatttaataa taagactagt
     4921 tgatagagta agtgtttctc cagcatgttt atcaccatta ttcattaatg taataacaat
     4981 taagatagcg ataccagcta taaccatcat gtaatgttta gttgtaaatg acgttttata
     5041 gttagaaatt ttcaataaat atggaacgat accaatgatt aatccaccaa agaaaaacat
     5101 agttggaata tggtgttggc ttaataaata attaaaaaga ttacttagtg atcccattgc
     5161 cagtaacatt ccaattataa tggggattaa aaatgtaaaa cttggccaaa aacgtcgtga
     5221 gaatatgccg ctaattgaag cgataaattg attgtaaata cctaacaata atgcgatagt
     5281 cccaccgcta acaccaggta ccaagtcact cgttcccata gcaaaacctt ttagaatatt
     5341 aatccattta aattgttgca tgaataactc ctttcaaacg attggaataa aatcataaat
     5401 agcatcatac catattac
//
LOCUS       DAMZKC010000154         5414 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.156, whole genome shotgun sequence.
ACCESSION   DAMZKC010000154 DAMZKC010000000
VERSION     DAMZKC010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5414
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.156"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            27..1427
                     /locus_tag="POB55_002180"
     CDS             27..1427
                     /locus_tag="POB55_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6501127.1"
                     /translation="MDTSKQFRGDNRLLLGIVLGVITFWLFAQSLVNLVVPLQSTYSS
                     DVGTINIAVSLSALFAGLFIVGAGDVADKFGRVKITYVGLILNVVGSLLIIITPLPAF
                     LIIGRIIQGLSAACIMPSTLAIINEYYIGTRRQRALSYWSIGSWGGSGICTLFGGLMA
                     TYIGWRSIFVVSILLTLLAMYLIKHAPETKAEPIKGMKAEAKKFDVIGLVILVVTMLS
                     LNVIITQTSHFGLVSPLILGLIVVFICSLVGFVYYENKIKHPLVDFSIFKNRGYSGAT
                     ISNFLLNGVAGGALIVINTYYQQQLGFNSSQTGYISLTYLIAVLSMIRVGEKILSQHG
                     PKRPLLLGSGFTVIGLILLSLTFLPEMWYIVSSIVGYLLFGTGLGLYATPSTDTAVAS
                     APDDKSGVASGVYKMASSLGNAFGVAVSGTVYTVLAANLNLNLGGFTGMMFNTLLAIV
                     AFLVILLLVPKNQTNL"
     gene            1653..2588
                     /locus_tag="POB55_002181"
     CDS             1653..2588
                     /locus_tag="POB55_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDI6501128.1"
                     /translation="MKIAIAGSGALGSGFGAKLFQAGYDVTLIDGYTSHVEAVKQHGL
                     NITINGEAFELNIPMYHFNDQPDESIYDVVFLFPKSMQLKEVMEDMKPHIDNETIVVC
                     TMNGLKHEEVIAQYVAQSQIVRGVTTWTAGLESPGHSHLLGSGPVEIGELVDEGKENV
                     IKVADLLNEAELNGVISKDLYQSIWKKICVNGTANALSTVLECNMASLNESSYAKCLI
                     YKLTQEIVHVATIDNVHLNVDEVFEYLVDLNEKVGAHYPSMYQDLIVNNRKTEIDYIN
                     DAVATLGKQRHIEAPVNRFITDLIHTKESQRHAQD"
     gene            2910..3761
                     /locus_tag="POB55_002182"
     CDS             2910..3761
                     /locus_tag="POB55_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDI6501129.1"
                     /translation="MSKIFVTGATGLIGIKLVQRLKEEGHEVAGFTTSENGQQKLAAV
                     NVKAYIGDILKADTIDQALADFKPEIIINQITDLKNVDMAANTKVRIEGSKNLIDAAK
                     KHDVKKVIAQSIAFMYEPGEGLANEETSLDFNSTGDRKVTVDGVVGLEEETARMDEYV
                     VLRFGWLYGPGTWYGKDGMIYNQFMDGQVTLSDGVTSFVHLDDAVETSIQAIHFENGI
                     YNVADDAPVKGSEFAEWYKEQLGVEPNIDIQPAQPFERGVSNEKFKAQGGTLIYKTWK
                     DGMNPIK"
     gene            complement(3853..5349)
                     /locus_tag="POB55_002183"
     CDS             complement(3853..5349)
                     /locus_tag="POB55_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HDI6501130.1"
                     /translation="MTTKKLYFLSISIIILVAISIAIHITLNSNTKTRLTNDSQQQID
                     TIIEHDLQKGHIPGASILIVKNGKVFLNKGYGYQDVDKKVKASPTTKYEIASNTKAFT
                     GLAILKLAQEGRLNLNDAVSKHVPHFKMNYNGQNETITIKQLLAQTSGIPSDITSEDA
                     VTNKNNRLNDVTRAIMGDELHHKPGEEFEYSNMNYDLLGLIIQNVTKQSYTQYITDHW
                     LKPLQMKHTTFKQTNYKSKHDAIGYELQGSTPVVSKPEFNLWDTPSAYMMTSTEDLEH
                     WIKFQLNPPDKYKSLVQQSHKNLSSTIGEPNANAYASGWFTNNDEHLVFHSGTLDNFS
                     SFILLNPKQNYGIVVLANLNSEYVPKLVEHLNTQIVNHKRYSTVASILNQYKDQFNIV
                     TVLMTTLILLAFIFSAYRAWQMRHGQILLRRSKRIAVLSWLTLCLCIAIALILYALPY
                     LILGSNNWSFVLTWLPIEIKLALITTLIALFSTLIVILLFLHTKITKT"
BASE COUNT         1735 a          736 c         1076 g         1867 t
ORIGIN      
        1 ttaaacatag gaggcttatc taattcatgg acacatcaaa acaatttaga ggtgacaacc
       61 gattgctttt gggtatcgtt ttaggggtta ttaccttttg gctattcgcg cagtcacttg
      121 ttaatcttgt tgtcccatta caatcaacat atagtagtga cgttggaacg ataaatatcg
      181 ctgttagctt atctgcctta tttgctggtt tgtttatcgt aggtgctggt gatgttgctg
      241 ataaatttgg tcgcgtcaaa attacttatg taggattgat attaaatgtt gtaggttcat
      301 tactcatcat cattacacct ttgccagcat ttttaattat aggtagaata attcaaggtt
      361 tgtctgcagc atgtattatg ccatcaacac ttgctattat taacgaatat tatattggta
      421 caagaagaca acgtgcctta agctattggt ctattggttc ttggggtggt agtggtattt
      481 gtacgttgtt tggtggctta atggctacat atataggttg gcgttcaata tttgttgttt
      541 caattctatt aacattatta gcaatgtact taatcaaaca tgcacctgag actaaagcag
      601 aaccaatcaa aggtatgaaa gcagaagcta aaaagtttga cgttattggt ttagtcattt
      661 tagtagtgac gatgttaagt ttaaatgtaa tcatcacaca gacgtctcat tttggtttag
      721 tttcaccgtt aattctaggt ttaattgttg tgtttatctg ttcattagtt ggttttgtat
      781 attacgaaaa taaaataaag catccacttg ttgatttttc aatttttaaa aatagaggat
      841 acagtggtgc aactatttca aacttcttat taaatggtgt agcaggtggt gcacttatcg
      901 tcattaacac gtattatcaa caacaattag gatttaattc ttcgcaaacg ggttatattt
      961 cattaacgta tttaatagca gtgttgtcaa tgattcgtgt aggtgaaaag attttatctc
     1021 aacatggtcc gaagcgccca ctattactag gaagtggctt tacagtgatt gggttaatct
     1081 tattgtcgtt aacattttta ccagaaatgt ggtatatcgt atctagtata gttggatatt
     1141 tattgtttgg tactggttta ggattatatg ctacaccatc aactgataca gcagttgcta
     1201 gtgcgccaga tgataagtcg ggtgttgctt caggtgtgta taaaatggcg tcatcattag
     1261 gaaatgcatt tggagtagca gtatctggta cggtttatac tgtgttagca gctaatttaa
     1321 atttgaactt aggtggtttc acaggtatga tgtttaatac cttgctagca attgttgcat
     1381 ttttagtcat tttactatta gttcctaaaa atcaaacgaa tttgtaaaac tgaaatgaaa
     1441 gcaagttatt atgtacggat tttaaaggaa attttgtgaa agtaagttta tcatacacac
     1501 ttaatgttac gtattgacgt ttaatgttag gtgtgttctt ttatagacga taaaagctgt
     1561 gtgcatatta agcgaatgat tttcaaattg acgctaatat gcgaaagtag tatttttaaa
     1621 atgaacaaca acgatgaaga ggggtttata ggatgaaaat tgcaattgct ggatcgggtg
     1681 cattaggtag tggctttggt gccaaactat ttcaagcagg atatgatgtc acacttattg
     1741 acggatatac atctcatgtt gaagcggtta agcaacatgg attaaatata acgattaatg
     1801 gagaggcatt cgagttaaac attccgatgt atcattttaa tgatcaaccg gacgaaagca
     1861 tttacgatgt tgtctttcta tttccaaagt ctatgcaatt aaaagaagtg atggaagata
     1921 tgaagccaca tattgataat gaaacgatcg tcgtatgtac gatgaatggt ctgaagcatg
     1981 aagaagtcat tgcgcagtat gttgctcaat cacaaattgt cagaggtgtt acgacttgga
     2041 cggcaggtct tgaaagccct ggacacagtc atttacttgg tagtggacca gttgaaatag
     2101 gtgaactagt ggatgaaggt aaagaaaatg ttataaaagt tgctgattta cttaacgaag
     2161 cggaattgaa tggtgtcatt agtaaagatt tataccaatc gatttggaaa aagatttgtg
     2221 ttaatggtac ggcaaatgca ttaagcacag tgttggagtg taatatggca tcgctgaatg
     2281 aaagtagtta tgcgaagtgt ttgatttata aattaacgca agaaatagtg catgtagcga
     2341 cgattgataa tgttcattta aatgttgatg aagtatttga atatttagtt gatttaaatg
     2401 aaaaagttgg tgcgcattat ccatccatgt atcaagattt aattgttaat aatagaaaaa
     2461 ctgaaattga ttatattaat gacgcagttg caacattggg taaacaacgt catattgaag
     2521 cgccagtcaa tcgctttatt actgatttaa ttcatactaa agaaagtcag cgacacgcac
     2581 aggattaatt ggtgttttag gtatgattac aaggaaatga caatcacgtg atattacggt
     2641 cattattaag attgaaatgt aataaataaa gaacagcagt aaggtacttt caaattgaaa
     2701 tgatcttggt gctgtttttc ttgattgatt ttcgtcataa ttcagatttg tcataggtct
     2761 acgacatact attagtattt actagacagt ttttacgacg acactttgaa aaattttgag
     2821 gcaaatcatt tggaagtctc acgtgaattt tgtaaactca tcaagcaagt aattatatta
     2881 aaaagacaaa tagagaaaag gtgtttataa tgagtaaaat ttttgtaact ggtgcaacgg
     2941 gccttattgg cattaaatta gttcaaagac taaaagaaga ggggcatgag gttgctggtt
     3001 ttactacatc tgagaatggt caacaaaagc tagctgctgt taatgtaaaa gcatatattg
     3061 gtgatatatt aaaagctgat actattgatc aagcgttagc agattttaaa ccagaaatca
     3121 ttatcaacca aattacggat ttaaaaaatg ttgatatggc agcaaatacg aaagtacgta
     3181 ttgaaggttc taaaaactta attgatgcgg cgaaaaagca tgacgttaag aaagtaattg
     3241 cccaaagtat tgcctttatg tatgaacctg gcgaaggatt agcaaatgag gaaacttcac
     3301 ttgattttaa ctcaactggt gatagaaaag taacggttga tggtgtggtt ggtctagaag
     3361 aagaaacggc tcgtatggat gaatacgttg ttttacgttt tggttggtta tatggcccag
     3421 gtacttggta cggaaaagat ggcatgattt ataatcaatt tatggatggt caagtgacac
     3481 tttcagatgg cgtaacatca tttgtgcatc ttgatgatgc agttgaaaca tctattcaag
     3541 ctattcattt tgaaaatggt atctataatg tagcagatga tgcacctgtt aaaggttctg
     3601 aatttgcaga atggtataaa gaacaacttg gtgttgaacc aaatattgat attcaacctg
     3661 cgcaaccatt tgaacgtggc gtaagcaatg agaagtttaa agcgcaaggt ggtactctga
     3721 tttataaaac ttggaaagac ggcatgaatc caattaaata ataatttatc cgtttaatat
     3781 acaaagaata aagacttggt cgaatcgtgg atgatatatt atcaaacgca cggctcgaac
     3841 aagtcttttt tattatgtct tcgttatctt tgtatgaagg aataacagaa ttacaattaa
     3901 tgtactgaat aatgcaatta atgttgtgat tagtgctaat ttaatttcta ttggtagcca
     3961 agtcagtaca aaagaccaat tattgctacc gagaatgaga tatggtaatg catataatat
     4021 gagcgctata gcgatacata aacataaagt tagccaactc aatacagcaa tccgttttga
     4081 tctacgcaaa agaatttgac catggcgcat ttgccaagca cgataagctg agaatataaa
     4141 tgctaataaa ataagtgttg tcatcaaaac ggtaacaata ttaaattggt ctttatattg
     4201 attgagtata gacgcaaccg tcgaatatcg cttgtgattt acaatttgtg tattaagatg
     4261 ctcaactaat ttgggtacat attccgaatt tagatttgca agcacaacaa ttccataatt
     4321 ttgttttgga tttagtaaaa taaatgatga aaagttatcg agcgttcctg aatgaaacac
     4381 taaatgttca tcattattgg taaaccagcc cgaagcatat gcattggcat taggttcacc
     4441 aattgttgat gataaatttt tatgtgattg ttgaactaat gatttgtatt tatcaggtgg
     4501 attaagttgg aattttatcc aatgttccaa atcttcagtt gatgtcatca tatatgctga
     4561 tggtgtatcc caaaggttaa attcaggttt agagacgaca ggtgtcgaac cttgtaattc
     4621 gtagccaata gcatcatgtt ttgatttgta atttgtttgt ttaaatgtcg tgtgtttcat
     4681 ttgaagtggt ttaagccaat ggtctgttat atattgcgta tatgattgct ttgtgacatt
     4741 ttggataatt aaacctaata aatcatagtt catatttgag tattcaaatt cttctccggg
     4801 cttatgatgt aattcatcac ccataattgc acgggttaca tcatttaaac gattattttt
     4861 atttgttacg gcgtcttcgc ttgtaatatc actaggtata ccacttgttt gagccaaaag
     4921 ttgcttaatc gtaatagttt cattttgacc attatagttc attttaaaat gaggcacatg
     4981 tttggatacg gcatcattta agtttaatcg accttcttga gctaatttta aaattgcaag
     5041 acctgtgaaa gctttcgtat tagaagcaat ttcatacttt gttgtgggag aagctttgac
     5101 ttttttatca acatcttgat aaccataacc tttatttaaa aaaactttgc cattttttac
     5161 tattaaaatt gatgctccag gaatgtgtcc cttttgtaaa tcatgctcga taattgtatc
     5221 tatttgttgt tgcgaatcat tggttaaccg tgtcttcgta ttgctattta atgttatatg
     5281 tatagcaatt gaaatggcga ctaaaatgat aatagaaatg gatagaaaat acagtttttt
     5341 agtagtcata aacgttcctt tttatatgtt aaatttactt aagtgcctaa tgagatttat
     5401 tttattatac cagt
//
LOCUS       DAMZKC010000155         5322 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.157, whole genome shotgun sequence.
ACCESSION   DAMZKC010000155 DAMZKC010000000
VERSION     DAMZKC010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5322
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.157"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            33..2651
                     /gene="mutS"
                     /locus_tag="POB55_002184"
     CDS             33..2651
                     /gene="mutS"
                     /locus_tag="POB55_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDI6501131.1"
                     /translation="MSNVTPMMQQYLKIKSEYQDCLLFFRLGDFYEMFYEDAKEASRV
                     LEITLTKRDAKKENPIPMCGVPYHSADSYIDTLVNNGYKVAICEQMEDPKQTKGMVRR
                     EVVRIVTPGTVMEQGGVDDKQNNYILSFVMNQPEIALSYCDVSTGELKVTHFNDEATL
                     LNEITTINPNEVVINDNISDNLKRQINMVTETITVRETLSSEIYSVNQTEHKLMYQAT
                     QLLLDYIHHTQKRDLSHIEDVVQYAAIDYMKMDFYAKRNLELTESIRLKSKKGTLLWL
                     MDETKTPMGARRLKQWIDRPLISKEQIEARLDIVDEFSAHFIERDTLRTYLNQVYDIE
                     RLVGRVSYGNVNARDLIQLKHSISEIPNIKALLNSMNQNTLVQVNQLEPLDDLLDILE
                     QSLVEEPPISVKDGGLFKVGFNTQLDEYLEASKNGKTWLAELQAKERQRTGIKSLKIS
                     FNKVFGYFIEITRANLQNFEPSEFGYMRKQTLSNAERFITDELKEKEDIILGAEDKAI
                     ELEYQLFVQLREEVKKYTERLQQQAKIISELDCLQSFAEIAQKYNYTRPSFSENKTLE
                     LVESRHPVVERVMDYNDYVPNNCRLDNETFIYLITGPNMSGKSTYMRQVAIISIMAQM
                     GAYVPCKEAVLPIFDQIFTRIGAADDLVSGKSTFMVEMLEAQKALTYATEDSLIIFDE
                     IGRGTSTYDGLALAQAMIEYVAETSHAKTLFSTHYHELTTLDQALPSLKNVHVAANEY
                     KGELIFLHKVKDGAVDDSYGIQVAKLADLPEKVISRAQVILSEFEASAGKKSSISNLK
                     MVENEPEINQENLNLSVEETTDTLSQKDFEQASFDLFENDQESEIELQIKNLNLSNMT
                     PIEALVKLSELQNQLK"
     gene            2664..4673
                     /gene="mutL"
                     /locus_tag="POB55_002185"
     CDS             2664..4673
                     /gene="mutL"
                     /locus_tag="POB55_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HDI6501132.1"
                     /translation="MGKIKELQTSLANKIAAGEVVERPSSVVKELLENAIDAGATEIS
                     IEVEESGVQSIRVVDNGSGIEAEDLGLVFHRHATSKLDQDEDLFHIRTLGFRGEALAS
                     ISSVAKVTLKTCTDNANGNEIYVENGEILNHKPAKAKKGTDILVESLFYNTPARLKYI
                     KSLYTELGKITDIVNRMAMSHPDIRIALISDGKTMLSTNGSGRTNEVMAEIYGMKVAR
                     DLVHISGDTSDYHIEGFVAKPEHSRSNKHYISIFINGRYIKNFMLNKAILEGYHTLLT
                     IGRFPICYINIEMDPILVDVNVHPTKLEVRLSKEEQLYQLIVSKIQEAFKDRILIPKN
                     NLDYVPKKNKVLHSFEQQKIEFEQRQNTENNQEKTFSSEESNSKPFMVENQNDEIVIR
                     EDSYNPFVTKTSESLIADDESSGYNNTREKDEDYFKKQQEILQEMDQTFDSNDDTSVQ
                     NYENKASDDYYDVNDIKGTKSKDPKRRIPYMEIVGQVHGTYIIAQNEFGMYMIDQHAA
                     QERIKYEYFRDKIGEVTNEVQDLLIPLTFHFSKDEQLVIDQYKNELQQVGIMLEHFGG
                     HDYIVSSYPVWFPKDEVEEIIKDMIELILEEKKVDIKKLREDVAIMMSCKKSIKANHY
                     LQKHEMSDLIDQLREAEDPFTCPHGRPIIINFSKYELEKLFKRVM"
     gene            4679..5221
                     /locus_tag="POB55_002186"
     CDS             4679..5221
                     /locus_tag="POB55_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HDI6501133.1"
                     /translation="MNQVNNNILPAIRNIKDLEKLIKTDYKMCVLLDMHIGHIKSIME
                     LLKQNHIECFIHIDLIKGLSHDEFASEFIIQQYKPKGIVSTKSKVIKKAKSLNTLTIF
                     RVFIIDSQALKRSIDLIKKVEPDFVEVLPGVASKAIHHIQKETNTQVIAGGLINTIDE
                     VNEAVKNGAKYVTTSYDKLW"
BASE COUNT         2098 a          694 c          983 g         1547 t
ORIGIN      
        1 ttaaaaagag acaagggttg tgaagaaata atatgtctaa tgttacacca atgatgcagc
       61 aatatttaaa aataaaatca gaataccaag attgcttatt attttttaga ctaggtgatt
      121 tctatgaaat gttttatgaa gatgccaagg aggcatcacg tgtacttgaa attactttaa
      181 ctaaaagaga tgctaaaaaa gaaaatccaa ttccgatgtg tggtgttccg tatcattctg
      241 cagatagtta tatagataca cttgttaata atggatataa agtagctatt tgtgaacaga
      301 tggaagatcc gaaacaaacg aaaggtatgg ttagacgtga ggtagtaaga attgtgactc
      361 caggaactgt gatggagcaa ggtggtgtag atgataaaca aaataactat attttaagtt
      421 ttgttatgaa tcaacctgaa attgcgctta gttactgtga tgtttctact ggcgaattaa
      481 aggttacaca ttttaatgat gaagcgactt tattaaatga aattacgacg ataaacccta
      541 acgaagttgt tatcaatgac aatatttccg ataatttaaa aagacaaatt aatatggtga
      601 cagaaacaat aacagtcagg gaaacgttat catcagaaat ctatagtgtg aatcaaactg
      661 aacataaatt aatgtatcaa gcgacacaat tattgctaga ttatattcat catacacaaa
      721 aacgtgattt atcgcatatc gaggatgttg ttcaatatgc agctatagat tatatgaaaa
      781 tggattttta tgctaagaga aaccttgagt taacggaaag cattcgatta aaatcaaaaa
      841 aaggaacgct actttggcta atggacgaaa cgaaaacacc aatgggagca cgccgcttaa
      901 aacaatggat agatagacca ctaataagta aagaacaaat tgaagcacga ttagatatcg
      961 ttgatgaatt tagtgctcat ttcatagaaa gagacacctt aagaacatat cttaatcaag
     1021 tgtatgatat tgaacgtctt gttgggcgtg ttagttacgg aaatgttaat gcgagagatt
     1081 taattcaact taaacattcc atttctgaaa taccgaatat taaagcatta ctaaattcta
     1141 tgaatcagaa tacgcttgta caagttaatc aactagaacc ccttgatgat ttacttgata
     1201 tattagaaca gagtttagta gaagaaccac caatttcagt taaagatggc ggactattca
     1261 aagttggttt taatacgcaa ttagatgaat atcttgaagc ttcaaaaaac ggaaaaacat
     1321 ggttagcaga attacaagcc aaagaaagac aacgtacagg aataaaatca ttgaaaataa
     1381 gctttaataa agtgtttggt tattttatag aaataacacg tgccaacttg caaaattttg
     1441 aaccaagtga atttggttat atgaggaagc aaacgttatc gaatgctgaa cgttttataa
     1501 ctgatgaact taaagaaaaa gaagatatca ttttaggtgc ggaagacaaa gccatcgaat
     1561 tagaatatca attatttgtt cagctacgtg aagaagttaa aaaatatact gaacgtttac
     1621 aacaacaagc taaaattatt tcagagctag attgtttaca gagctttgca gaaattgctc
     1681 aaaaatataa ttacactagg ccttcattta gtgaaaataa aacattagaa ttagtggaat
     1741 ctaggcaccc agtagtggaa agagtaatgg attataatga ctatgtgcct aataattgtc
     1801 gattagataa tgaaacattt atatatttaa ttacaggtcc gaatatgtct ggtaaatcga
     1861 catatatgag acaagttgcc ataattagta taatggccca aatgggagct tatgtccctt
     1921 gtaaagaggc agtgttacct atatttgatc aaatattcac tagaataggt gcggcagatg
     1981 atttggtttc aggtaagagt acgtttatgg tagaaatgct agaagcacaa aaggcattaa
     2041 cttatgcaac agaggatagt ttgattattt tcgatgaaat tggacgtggt acttcaacgt
     2101 atgacggttt agctttagcg caggcaatga tagagtatgt agctgaaaca tcacatgcta
     2161 aaacgttatt ttcaacacat tatcatgaat tgacaacatt agatcaagca ttaccaagtc
     2221 taaaaaatgt tcacgtcgct gctaatgaat ataaaggtga acttatattc ttgcataaag
     2281 tcaaagatgg tgcagttgac gatagttatg gtattcaagt tgcgaaatta gctgatttac
     2341 ctgaaaaagt tattagcaga gcacaagtga ttctaagcga gtttgaagcg tctgctggta
     2401 aaaaatcatc gatatcaaat ttaaaaatgg tcgaaaatga acctgaaatt aatcaagaaa
     2461 atttaaactt aagtgttgaa gaaacaactg atactttatc tcaaaaagac tttgaacaag
     2521 catcatttga tttgtttgaa aatgatcaag aaagcgagat tgaactacaa attaaaaatt
     2581 tgaatttatc taatatgaca ccaattgagg cattggtgaa gttaagtgaa ttacaaaatc
     2641 aattaaaata gaggtgttgc aaaatgggga aaattaaaga actccaaacc tcattagcaa
     2701 ataaaatcgc agcaggtgaa gtagttgaaa gaccgagttc tgttgtgaaa gaactgttgg
     2761 aaaatgctat agatgcaggc gctacagaaa taagcattga agtagaggaa tctggcgtcc
     2821 aatctattcg cgtagtcgat aatggaagcg gaattgaagc ggaagactta ggattagtat
     2881 ttcatagaca tgcgactagt aaattagatc aagatgaaga tttatttcat attaggacat
     2941 taggattccg tggtgaagca ctagccagta tttcatcagt tgctaaagta acattgaaga
     3001 cttgcacgga taatgctaat ggaaatgaaa tatatgtaga aaatggtgaa atattaaatc
     3061 ataagcctgc aaaagcgaaa aaaggaacag atatacttgt agaatcatta ttttataata
     3121 caccagcacg tttaaaatat attaaaagtt tatacactga actaggtaaa ataacagata
     3181 ttgtcaacag aatggcaatg agccatccgg acattcgaat agcactcatt tcagatggca
     3241 aaacaatgtt aagtacaaat ggttcaggac gaactaatga agtgatggca gagatttatg
     3301 ggatgaaagt tgcacgagat ttagtacata tatctggaga tacaagtgat tatcacattg
     3361 aaggttttgt tgcaaagcct gaacattcta gaagtaataa gcactatatt tctattttta
     3421 ttaatggacg atacattaaa aactttatgc taaataaagc gattttagaa ggctatcata
     3481 cactcttaac aataggtagg ttcccgattt gttatattaa tattgaaatg gatccaatct
     3541 tagtagacgt aaatgttcat ccaacaaaac tagaagtgcg tttatcaaaa gaagagcaac
     3601 tatatcaatt gatagtgagc aaaatacaag aagcatttaa agaccgtata ttaattccta
     3661 aaaataactt ggattatgtg ccgaaaaaaa ataaagtgtt acattcattc gaacaacaaa
     3721 aaatcgaatt tgaacaaaga caaaacacag agaataatca agagaagacg ttttcatctg
     3781 aagaaagtaa cagtaagcca tttatggtag aaaatcaaaa cgatgaaata gttataagag
     3841 aagattcata taatccattc gtaacgaaaa cgtctgaaag tttaatagct gatgatgaat
     3901 cttccggtta taataataca cgtgaaaaag atgaagacta cttcaaaaag caacaagaaa
     3961 ttctacaaga aatggatcaa acatttgatt cgaatgacga tacatctgtg caaaattatg
     4021 agaataaagc gtctgatgat tattatgatg taaacgatat taaaggaaca aaaagtaaag
     4081 accctaaacg aagaattcca tatatggaaa ttgttggcca agtacatgga acgtatatta
     4141 ttgctcaaaa tgaatttggc atgtacatga ttgaccagca tgcagctcaa gaaagaataa
     4201 aatatgaata ttttcgagat aaaataggtg aagttaccaa tgaagtacaa gatttattaa
     4261 tcccgttaac atttcatttt tcaaaagatg aacaattagt cattgatcaa tataaaaatg
     4321 agcttcaaca agtaggtatc atgttagaac attttggtgg tcatgattat attgtaagta
     4381 gctatccagt ttggttccct aaagatgaag tagaagaaat tattaaagat atgattgagc
     4441 taattttgga agagaaaaaa gtagatatca aaaaattacg tgaagatgta gcaatcatga
     4501 tgtcatgtaa aaaatctatt aaagcgaatc attatttaca aaaacatgaa atgtctgatt
     4561 taattgatca attaagagaa gcggaagatc catttacatg tccacatggt cgtccaatta
     4621 tcattaattt ttcaaaatac gaattagaaa aattatttaa gcgtgtgatg tagagaggat
     4681 gaatcaagtg aataacaaca tattgcctgc cataagaaac attaaagatt tagagaaact
     4741 gattaaaaca gattataaaa tgtgtgtgct tctagatatg catataggac atataaaaag
     4801 tattatggaa ttgctgaagc aaaatcatat agagtgtttt attcatatag atttgataaa
     4861 aggtttaagc cacgatgaat ttgcaagtga atttattatt cagcaataca agccaaaagg
     4921 tatcgtatcg actaaatcta aagtaataaa aaaagctaaa tcattaaata ctttaacgat
     4981 ttttagagta tttattattg atagtcaagc attgaaacgc agtatagatt tgataaaaaa
     5041 agttgaacct gattttgttg aagtacttcc aggtgttgcg agtaaagcga ttcatcatat
     5101 tcagaaagaa acaaacacac aagtcattgc aggtggccta attaatacaa tagatgaagt
     5161 caatgaagct gttaaaaatg gagcgaaata tgtaacaact agttatgata aactttggta
     5221 aatcataagt ctgcaaaaat ttcatttttc acgagattta gtcatataac atattaaaaa
     5281 agccgttttc atatgggata tgtaataagg tatttaaggt at
//
LOCUS       DAMZKC010000156         5237 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.158, whole genome shotgun sequence.
ACCESSION   DAMZKC010000156 DAMZKC010000000
VERSION     DAMZKC010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5237
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.158"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(116..763)
                     /locus_tag="POB55_002187"
     CDS             complement(116..763)
                     /locus_tag="POB55_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDI6501134.1"
                     /translation="MKFDNYIFDFDGTLADTKKCGEVATQSAFKACGLTEPSSKEITH
                     YMGIPIEESFLKLADRPLDEAALAKLIDTFRHTYQSIEKDYIYEFAGITEAITSLYNQ
                     GKKLFVVSSKKSDVLERNLSAIGLNHLITEAVGSDQVSAYKPNPEGIHTIVQRYNLNS
                     QQTVYIGDSTFDVEMAQHAGVPSAAVTWGAHDARSLLHSNPDFIINDPSEINTVL"
     gene            complement(876..1424)
                     /gene="hxlB"
                     /locus_tag="POB55_002188"
     CDS             complement(876..1424)
                     /gene="hxlB"
                     /locus_tag="POB55_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-3-hexuloisomerase"
                     /protein_id="HDI6501135.1"
                     /translation="MAEFSDYQLILDELKMTLSHVEADEFSTFASKILHAEHIFVAGK
                     GRSGFVANSFAMRLNQLGKQAHVVGESTTPAIKSNDVFVIISGSGSTEHLRLLADKAK
                     SVGADIVLITTNKDSAIGNLAGTNIVLPAGTKYDEQGSAQPLGSLFEQASQLFLDSVV
                     MGLMTEMNVTEQTMQQNHANLE"
     gene            complement(1426..2058)
                     /gene="hxlA"
                     /locus_tag="POB55_002189"
     CDS             complement(1426..2058)
                     /gene="hxlA"
                     /locus_tag="POB55_002189"
                     /EC_number="4.1.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hexulose-6-phosphate synthase"
                     /protein_id="HDI6501136.1"
                     /translation="MELQLAIDLLNKEDAAELANKVKDYVDIVEIGTPIIYNEGLPAV
                     KHMADNISNVKVLADMKIMDAADYEVSQAIKFGADVITILGVAEDASIKAAIEEAHKN
                     NKQLLVDMIAVQDLEKRAKELDEMGADYIAVHTGYDLQAEGQSPLESLRTVKSVIKNS
                     KVAVAGGIKPDTIKEIVAESPDLVIVGGGIANADDPVEAAKQCRAAIEGK"
     gene            complement(2135..2893)
                     /gene="nagB"
                     /locus_tag="POB55_002190"
     CDS             complement(2135..2893)
                     /gene="nagB"
                     /locus_tag="POB55_002190"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDI6501137.1"
                     /translation="MKVLNLGSKKQASFYVACELYKEMAFNQHCKLGLATGGTMTDLY
                     EQLVKLLNKNQLNVDNVSTFNLDEYVGLTASHPQSYHYYMDDMLFKQYPYFNRKNIHI
                     PNGDADDMNAEASKYNDVLEQQGQRDIQILGIGENGHIGFNEPGTPFDSVTHIVDLTE
                     STIKANSRYFKNEDDVPKQAISMGLANILQAKRIILLAFGEKKRAAITHLLNQEISVD
                     VPATLLHKHPNVEIYLDDEACPKNVAKIHVDEMD"
     gene            3169..3531
                     /locus_tag="POB55_002191"
     CDS             3169..3531
                     /locus_tag="POB55_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YojF family protein"
                     /protein_id="HDI6501138.1"
                     /translation="MLEPIKEQEVIDLLASFEHKPVYLHVETTNGAYANHFDQRVFNA
                     GTFLRNIQVTYTHAQLKGGNKEPYRIGLKLSNGGWVYVQGLTHFEVNEHDEFLIAGFN
                     YEGQLAAALQISERPFNL"
     gene            3545..4210
                     /gene="bshB2"
                     /locus_tag="POB55_002192"
     CDS             3545..4210
                     /gene="bshB2"
                     /locus_tag="POB55_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB2"
                     /protein_id="HDI6501139.1"
                     /translation="MTDERHVLVIFPHPDDETFSSAGTLASYIQKGIPVTYACLTLGQ
                     MGRNLGNPPFATRESLPSIRERELEEACKVIGITDLRKMGLRDKTVEFEPYEHIDGMI
                     KSLIDDTNPSLIISFYPGYAVHPDHEATADAVIRTVERMPKEERPRLTLVAFSNDATE
                     ALGEPDIQNDITDFKELKIKAFEAHASQTGPFLKQLASPEIDGKQHSFLTVEPYWTYH
                     FKS"
     gene            4223..5101
                     /locus_tag="POB55_002193"
     CDS             4223..5101
                     /locus_tag="POB55_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE2"
                     /protein_id="HDI6501140.1"
                     /translation="MTEFDLSTREGRWKHFGSVDPIEGTKPTTKNEMTDLQSTHKDFL
                     FEIEEVGIKNLVYPVLVDQYQTAGTFSFSTSLTKDEKGINMSRIIESVEKHYDNGIEL
                     EFNTLYQVLRTLQTNMKQNAAGVDVSGKWFFDRYSPTTNIKAVGNADVTYGLAIDGDK
                     VTRKELTIEATVTTLCPCSKEISEYSAHNQRGVVTVKTYINKDQDIVDDYKNKILDAM
                     EANASSILYPILKRPDEKRVTERAYENPRFVEDLIRLIAADLVEFDWLDGFDIECRNE
                     ESIHQHDAFAKLKYRK"
BASE COUNT         1685 a          946 c          820 g         1786 t
ORIGIN      
        1 taatgtttaa taaatattta ttttaaattc ctaatctaaa gcgaaatatt tctatgtata
       61 atctaatatt tatgaatatg cattttaacc atggtattct ctgttttaac aaattttata
      121 atactgtatt aatttctgat ggatcattga ttataaaatc cggatttgaa tgaagtaatg
      181 accttgcatc atgtgctccc catgttactg ctgcagatgg tacaccagca tgttgcgcca
      241 tctcaacatc aaatgttgaa tcaccaatat aaaccgtttg ttggctattt aaattgtagc
      301 gttgcacaat tgtgtgtatg ccttcaggat ttggtttata tgcacttact tgatcggatc
      361 caacagcttc ggtaatcaag tgatttaatc caatagccga taaatttctt tctaatacat
      421 cactcttctt actagacacc acgaaaagtt ttttcccttg gttatacaaa cttgtaatgg
      481 cttcagttat acccgcaaat tcataaatat agtccttttc aatagattga tatgtatgtc
      541 taaatgtatc gattaacttt gctaatgctg cttcatctaa tggtcggtct gctaatttta
      601 aaaatgattc ttcaataggt attcccatat aatgcgttat ttctttagat gatggttccg
      661 ttaagccaca tgctttaaat gcactttgtg ttgctacttc accacatttt ttcgtgtctg
      721 ccaacgtacc atcaaaatca aaaatataat tgtcaaactt caatattaac acccctattc
      781 gtttattatt gatattatac tataatttat tatttaaata aaaaaataat cgataatatt
      841 tctgcctagg catcatatta tcgactatct ttattttatt ctaaattagc atgattttgt
      901 tgcatcgttt gttccgtaac attcatttca gtcatcaatc ccattacaac actatctaaa
      961 aataattgag atgcttgttc aaacaaactt cctaatggtt gtgccgagcc ttgttcatca
     1021 tattttgtac ctgcaggcaa aacgatgttc gtcccagcta gattgcctat tgcagaatct
     1081 ttatttgtag taattaatac gatgtcagca cctactgatt ttgctttgtc tgctaataat
     1141 cttaaatgtt ccgtggaacc tgaaccagag ataattacaa atacatcatt cgacttaatc
     1201 gcaggtgtcg ttgattctcc aacaacatgt gcctgtttgc cgagctgatt taagcgcatt
     1261 gcaaaactat tcgccacgaa tcctgaacgt cctttaccag ctacaaatat atgttcagca
     1321 tgtagtattt tggatgcaaa cgttgaaaac tcatccgctt caacatgtga caaagtcatc
     1381 tttaattcat ctagaattaa ttgatagtca ctaaattcag ccatattact taccttcgat
     1441 tgcagcgcga cattgttttg cagcttctac tggatcatct gcatttgcga ttccgccacc
     1501 aacaataaca agatcaggac tttcagcgac aatttcttta attgtatctg gtttaattcc
     1561 acctgctact gcaactttag aatttttaat aacagattta acggttctta aactttctaa
     1621 tggtgattgc ccttctgctt gtaaatcata accagtgtgt actgcaatat aatccgcacc
     1681 catttcatct agttcttttg cacgtttttc taaatcttga acagcaatca tatcaactag
     1741 taattgttta ttatttttat gagcttcttc aatagctgct ttaattgatg catcttctgc
     1801 aacacctagt attgtaatta catccgcgcc aaatttaatt gcttggctaa cttcataatc
     1861 agctgcatcc ataattttca tgtctgctaa tacttttaca ttactaatgt tgtctgccat
     1921 atgtttaact gctggtaaac cttcgttgta aatgattggc gtaccgattt ctacgatatc
     1981 tacataatct tttactttat ttgctaactc agccgcgtct tctttgttta ataaatcaat
     2041 tgctaattgt aattccacta aaaatcatcc ttccaatgct ttttatcttt ctattggaac
     2101 ctttcccgag gcatttctta attaaacatt gcaatcaatc catttcatcg acatgaattt
     2161 tcgcaacatt tttcgggcaa gcttcgtcgt ctaaatatat ctcaacattc gggtgtttgt
     2221 gaagtaatgt ggctggaaca tcaacagaaa tttcctgatt taataaatgt gtaatagcag
     2281 cacgtttctt ttcaccaaat gcgagtaaaa tgatacgttt ggcttgaaga atattagcaa
     2341 gtcccatcga aatggcttgc tttggaacat catcttcgtt tttaaaatat cgactattag
     2401 ccttaatagt actttcagtc aaatcaacga tatgagtaac gctgtcaaac ggcgtaccag
     2461 gttcattaaa tccaatatga ccattttctc caatacctaa aatttgaata tcacgttgac
     2521 cttgttgttc taaaacgtca ttatattttg acgcttccgc attcatatca tcggcatctc
     2581 catttggaat atgaatgttc tttctattaa aataaggata ttgtttgaaa agcatgtcat
     2641 ccatataata gtgataactt tgcggatgtg atgcggttaa acctacatat tcgtctaaat
     2701 taaacgtgga tacattgtct acgtttaact gatttttatt taataactta acaagttgct
     2761 catacaaatc tgtcattgta ccaccagttg ctaaaccaag tttacagtgc tgattaaatg
     2821 ccatctcttt atataactca catgcaacat agaatgatgc ttgttttttc gatcctaagt
     2881 ttaatacttt cattgtttac atgcctccac aaatttaata cattttaaaa acttatgaat
     2941 tcattcatac accattaata tagcatcatt tttaatgttt aacacttttc aaattccaac
     3001 aaatttttga tttttaaaag taaaaaacta atgctgtatg attgcgtaat cctgagtaat
     3061 tacccttcat gaaaattaat ttataaataa ttgagcaatc gtatacaatt attgaatagt
     3121 ttttagtaaa atagaaagat aaacacattg atagtgtaag gagtgaacgt gttggaacca
     3181 attaaagaac aagaagttat agatttactt gcttcttttg aacataaacc tgtgtatctg
     3241 catgttgaaa cgacaaatgg ggcctatgcg aaccatttcg atcaacgtgt ttttaatgct
     3301 ggtacatttt taagaaacat acaagtaaca tatacgcatg cacaactaaa aggtggtaat
     3361 aaagaacctt atcgcattgg tcttaaacta tcaaatggtg gttgggttta tgtacaaggt
     3421 ctaacgcatt ttgaggtcaa tgaacatgat gaatttttaa ttgcaggttt taattacgaa
     3481 ggtcaactcg ctgcagcact tcaaattagt gagcgaccat ttaatttata gaggaggcat
     3541 caacatgact gacgaaagac atgtattagt aatatttcca catcctgatg atgaaacctt
     3601 ctcatctgca ggcacattag caagttatat ccaaaaaggc atacctgtaa catatgcatg
     3661 tttaacccta ggacaaatgg gcagaaattt aggtaatccc ccatttgcaa cacgcgaatc
     3721 attaccaagt attcgtgagc gtgaactaga agaagcttgt aaagttattg gtattactga
     3781 tttaagaaaa atgggtttac gtgacaaaac tgtcgaattt gaaccctacg aacatataga
     3841 tggtatgatt aaatctttaa tcgatgatac aaatccatca ttgattattt cattttatcc
     3901 tggatatgca gtacatcctg atcacgaagc aacagcagac gctgtaattc gaacggttga
     3961 acgcatgcct aaagaagaac gccctcgttt aactttagtt gcgttcagta atgatgcaac
     4021 cgaagctctt ggagaacctg atattcaaaa tgatattaca gattttaaag aacttaaaat
     4081 taaggcattt gaagcacatg cttctcaaac aggtccattt ttaaaacaat tagcaagtcc
     4141 tgaaatcgat ggtaaacaac acagcttttt aacagtcgaa ccatattgga catatcactt
     4201 taaatcttaa cggaggaaaa aaatgactga atttgattta tcgacacgag aaggtcgttg
     4261 gaaacacttt ggttccgtag atcctattga aggcacgaaa cctactacta agaacgaaat
     4321 gacagactta caaagtacac ataaagattt tttatttgaa atagaagaag ttggtattaa
     4381 aaacttagtg taccctgtac ttgttgacca gtatcaaaca gctggcacat ttagtttttc
     4441 aacaagttta acaaaagacg aaaaaggtat taatatgagc cgtataattg aaagtgttga
     4501 aaagcactat gataatggta ttgaacttga atttaataca ctttatcagg tgttacgcac
     4561 tttacaaacg aatatgaaac aaaatgcggc aggcgttgat gtttctggga aatggttctt
     4621 tgatcgctat agtccaacca caaatattaa agcagttggt aatgccgatg tcacttatgg
     4681 attagcaatt gacggtgata aagttacacg taaagaactt actattgaag caactgtcac
     4741 aacgctatgc ccttgttcaa aagaaataag tgagtattca gcgcacaacc aacgtggtgt
     4801 tgtcactgtt aaaacatata ttaataaaga tcaagacata gttgatgact ataaaaataa
     4861 aattttagat gcaatggaag ctaatgcaag ttcaattcta taccctattt taaaacgtcc
     4921 tgatgaaaaa cgtgtgacag agcgtgcata tgaaaatcca cgttttgtcg aagacttaat
     4981 tcgtttaatt gccgcggact tagttgaatt tgattggtta gacggtttcg atatcgaatg
     5041 tcgtaatgaa gagtctatcc atcaacatga tgctttcgct aaattaaagt atcgcaaata
     5101 atatcatatg attaattcaa acgcgtaaca gtagttgcgc gtttttatta taccttctag
     5161 agttgtatat ctcttttata aatcttctca tttgatgtgc ctgagtcata aatcaatttc
     5221 ctaaactcta caatgat
//
LOCUS       DAMZKC010000157         5215 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.159, whole genome shotgun sequence.
ACCESSION   DAMZKC010000157 DAMZKC010000000
VERSION     DAMZKC010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5215
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.159"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..148
                     /locus_tag="POB55_002194"
     CDS             <1..148
                     /locus_tag="POB55_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501141.1"
                     /translation="VIYEVLTSGNQPFTYELPKDLSSHNARKYLEFISQKLDGDKLTK
                     EDSL"
     gene            248..712
                     /locus_tag="POB55_002195"
     CDS             248..712
                     /locus_tag="POB55_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501142.1"
                     /translation="MKRLLGLLLVSTLVLSACGNDENQEESKKEVKSKEKKIEKEKEN
                     KSKKDKEKEVATQQQPDNQTVEQPQSQEQSVQQPQQQIPQNSVPQQNVQVQQNKKQKV
                     DLNNMPPTDFSTEGMSEQAQKQIEELSMQKDYHGLSQREYNDRVSEIINNDN"
     gene            771..1808
                     /locus_tag="POB55_002196"
     CDS             771..1808
                     /locus_tag="POB55_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDI6501143.1"
                     /translation="MKTRCYDGKKWQYEFKHEGKRYRKKGFRTKREANSAGLDKLNEL
                     RSGFNIDNYITLEEYFENWIKTYKQPVVKENTYRHYRNALQHIQKHKIGKMELSKINR
                     QVYQKFINDYSKEHAKETIRKTNGAIRSALDDALYDGLIFKNPAYKVNYKAGKPTKSE
                     QEKFISVTEYEILKDHVRKKRTRSSLALFIMICTGCRVSGARNIKIEHINQVKNTIFI
                     DERKTDTSPRYISIAKSDMKHIMDVISTFAISYDGYIFKEAGSIINLQAINNALKSAC
                     RVNNIPIITSHALRHTHCSYLLAKGVSIHYISKRLGHKNIAITTSVYSHLLEEKFNEE
                     DKKTTKILESM"
     gene            1859..2689
                     /gene="sph"
                     /locus_tag="POB55_002197"
                     /pseudo
     CDS             1859..2689
                     /gene="sph"
                     /locus_tag="POB55_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239616.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sphingomyelin phosphodiesterase"
     gene            complement(2927..3943)
                     /gene="lukG"
                     /locus_tag="POB55_002198"
     CDS             complement(2927..3943)
                     /gene="lukG"
                     /locus_tag="POB55_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukGH subunit G"
                     /protein_id="HDI6501144.1"
                     /translation="MIKQLYKNITICTLALSTTFTVLPATSYAKINSEIKAVSEKNLD
                     GDTKMYTRTATTSDSQKNITQSLQFNFLTEPNYDKETVFIKAKGTIGSGLRILDPNGY
                     WNSTLRWPGSYSVSIQNVDDNNNTNVTDFAPKNQDESREVKYTYGYKTGGDFSINRGG
                     LTGNITKESNYSETISYQQPSYRTLLDQSTSHKGVGWKVEAHLINNMGHDHTRQLTND
                     SDNRTKSKIFSLTRNGNLWAKDNFTPKNKMPVTVSEGFNPEFLAVMSHDKKDEGKSKF
                     VVHYKRSMDEFKIDWNRHGFWGYWSGENHVDKKEEKLSALYEVDWKTHNVKFVKVLND
                     NEKK"
     gene            complement(3965..5017)
                     /gene="lukH"
                     /locus_tag="POB55_002199"
     CDS             complement(3965..5017)
                     /gene="lukH"
                     /locus_tag="POB55_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukGH subunit H"
                     /protein_id="HDI6501145.1"
                     /translation="MKNKKRVLIASSLSCAILLLSAATTQANSAHKDSQDQNKKEHVD
                     KSQQKEKRNVTNKDKNSTVPDDIGKNGKITKRTETVYDEKTNILQNLQFDFIDDPTYD
                     KNVLLVKKQGSIHSNLKFESHKEEKNSNWLKYPSEYHVDFQVKRNPKTEILDQLPKNK
                     ISTAKVDSTFSYSSGGKFDSTKGIGRTSSNSYSKTISYNQQNYDTIASGKNNNWHVHW
                     SVIANDLKYGGEVKNRNDELLFYRNTRIATVENPELSFASKYRYPALVRSGFNPEFLT
                     YLSNEKSNEKTQFEVTYTRNQDILKNRPGIHYAPPILEKNKEGQRLIVTYEVDWKNKT
                     VKVVDKYSDNKSFREG"
BASE COUNT         1846 a          823 c          782 g         1764 t
ORIGIN      
        1 tgtaatatac gaagttttaa ctagtggtaa tcaaccattc acttatgagt tacctaaaga
       61 tttatcgtca cataatgcgc gtaaatactt ggaatttatt tcacaaaaat tagatggcga
      121 taagttaacc aaagaagatt cattatgatt ttactaaaca aaaaaacgcc tactagtgtg
      181 aaaacgtatt gattaatagc gcctatatgg agttttaata taaaaagtaa gcaaaggaga
      241 aatgagaatg aaaagattgt taggtttatt attagtgagc acgttagtgt taagtgcatg
      301 tggaaatgat gagaatcagg aagaatctaa aaaagaagtt aaatcaaaag aaaagaaaat
      361 tgagaaggaa aaggaaaata aatcgaaaaa agataaggaa aaagaagttg caacacaaca
      421 acaaccagac aatcaaaccg ttgaacaacc ccaatcacaa gagcaatcgg ttcaacaacc
      481 gcaacaacag ataccacaaa atagtgttcc tcagcaaaat gtccaagttc aacaaaacaa
      541 aaagcaaaaa gttgatttaa ataatatgcc tcccactgat ttttctacag agggtatgtc
      601 tgagcaggct caaaaacaaa ttgaagagct ttcaatgcaa aaagactatc atggtctgtc
      661 acaaagagaa tacaatgata gagtttctga aattataaat aatgataatt gattccacag
      721 ggtagcccgc ctacccttat tattttttgc caattttgag gagggaacgc atgaaaacac
      781 gttgttacga tggtaaaaaa tggcaatatg aatttaagca tgaaggaaaa agataccgta
      841 agaaaggttt tagaacaaag cgtgaagcta attctgctgg actagacaag ttaaatgagt
      901 taagaagtgg ttttaatata gataactata taactcttga agaatacttc gaaaattgga
      961 ttaaaacata taaacaacct gttgttaaag aaaataccta ccgtcattat agaaatgcat
     1021 tacaacatat acaaaaacat aaaataggta aaatggagtt atcaaagata aatagacaag
     1081 tttatcagaa attcataaac gactattcaa aagaacacgc aaaagaaact ataagaaaaa
     1141 caaacggtgc tattcggtca gctttagatg acgcattata tgatggactt atttttaaaa
     1201 accccgctta taaagttaat tataaagccg gaaaacctac gaagtcagaa caagaaaaat
     1261 tcatctcggt aactgaatat gaaatactaa aagatcacgt cagaaagaag agaactcgtt
     1321 catcattagc gctattcata atgatttgta cgggttgtcg tgtcagtggt gcaagaaata
     1381 taaagattga gcatatcaac caagtgaaaa acactatatt tattgacgag cgaaaaaccg
     1441 atacttcccc tagatatatc agtatcgcta aatctgatat gaaacacatt atggacgtca
     1501 taagtacatt tgcaattagc tatgatggtt acattttcaa agaagccgga tctataatta
     1561 accttcaggc tatcaataat gctttgaaat cagcctgtag agtcaataat ataccaatta
     1621 ttacatcgca cgcattaaga cacactcatt gttcttattt actagcaaaa ggtgtatcta
     1681 tacattacat ttctaaaaga ttaggtcata aaaatatagc aataactaca tccgtgtatt
     1741 ctcatttgtt agaagaaaaa tttaatgaag aggacaaaaa aacaactaaa attttagaaa
     1801 gtatgtaatt tagggaccca ttagggactc caaacccaat aaatactgtt gttacaaggt
     1861 ttctatgtat ccaaactggg ggcaatataa acgcgctgat ttaatcggac aatcttctta
     1921 tattaaaaat aatgatgtcg taatattcaa tgaagcattt gataatggtg catcagacaa
     1981 attattaagt aatgtgaaaa aagaatatcc ttatcaaaca cctgtactcg gccgttctca
     2041 atcagggtgg gacaaaactg aaggtagcta ctcatcaact gttgcagaag atggtggcgt
     2101 agcgattgta agtaaatatc ctattaaaga aaaaatccag catgttttca aaagcggttg
     2161 tggattcgat aatgatagca acaaaggctt tgtttataca aaaatagaga aaaatggtaa
     2221 gaacgttcac gttatcggta cacatacaca atctgaagat tcacgttgtg gtgctggaca
     2281 tgatcgaaaa attagagctg aacaaatgaa agaaatcagt gactttgtta aaaagaaaaa
     2341 tatccctaaa gatgaaacgg tatatatagg tggtgacctt aatgttaata aaggcactcc
     2401 agagttcaaa gatatgctta aaaacttgaa tgtaaatgat gttctatatg caggtcataa
     2461 tagcacatgg gaccctcaat caaattcaat tgcgaaatat aattacccta atggtaaacc
     2521 agaacattta gactatatat ttacagataa agatcataaa caaccaaaac aattagtcaa
     2581 tgaagttgtg actgaaaaac ctaagccatg ggatgtatat gcgttcccat attactacgt
     2641 ttacaatgat ttttcagatc attatccaat caaagcctat agtaaatagt gctcaactaa
     2701 ctaataactc gcttcgttct aaaaggtcga agcgagttat attgttaaaa tttgaattga
     2761 cttacatttt aataaaatca tcttaacaat tttaattttt cattaataca agtctttact
     2821 ctacactcaa acaagattca tacactgcac atcataataa atctatctat tcaaatataa
     2881 ataaaagtta cctactacat tctatgtagc aggcaacttt tattacttat ttcttttcat
     2941 tatcattaag tacttttaca aacttcacat tatgtgtctt ccaatcaact tcatataatg
     3001 ctgataattt ttcttctttt ttatctacat ggttttcacc agaccaatag ccccagaaac
     3061 catgacgatt ccaatctatt ttaaactcat ccattgatct tttataatga acaacaaatt
     3121 ttgatttacc ttcatctttt ttatcatgtg acataacagc taaaaattct ggattaaacc
     3181 cttcagaaac agttacaggc atcttattct taggagtaaa attatctttc gcccataaat
     3241 ttccatttcg tgttaaagaa aagattttac ttttagttct attatcacta tcattagtta
     3301 attgtctcgt atggtcatgt cccatattat ttatcaaatg tgcttctact ttccaaccta
     3361 cacctttatg tgacgtagat tgatcaagta atgtacgata cgatggttgt tgataactaa
     3421 tcgtctctga ataattactc tcttttgtaa tatttccagt taaccctcca cgattaatcg
     3481 aaaaatctcc acctgtttta taaccatacg tatatttaac ttctcttgat tcatcctgat
     3541 tttttggtgc aaagtcagtc acatttgtat tgttgttgtc atcaacattt tgaattgaaa
     3601 ctgaataaga tccaggccat cttaatgtac tattccaata accatttggg tctagaattc
     3661 ttaaaccact acctattgtt cctttcgctt taataaatac tgtttcttta tcataattag
     3721 gttcagttaa gaagttaaat tgtaagcttt gagtaatatt tttttgacta tcacttgttg
     3781 tagctgtacg tgtatacatt ttagtatcac catcaagatt cttctcagaa acagctttaa
     3841 tttcagaatt aatttttgca taagaagtag ctggtaatac agtgaaagta gtcgatagtg
     3901 ctaacgtaca aattgtgata tttttatata gttgtttaat catttgtaat ccttcccttc
     3961 gattttatcc ttctctaaat gatttattat cagagtattt atcaacgact ttaactgttt
     4021 tatttttcca atcaacttca taagtgacaa ttaatctttg accttcttta tttttttcta
     4081 aaattggagg tgcataatgt attccaggtc tgtttttcaa aatatcttga tttcgtgtgt
     4141 atgttacttc aaattgcgtt ttctcatttg acttttcatt agataaataa gttaaaaatt
     4201 ctggattaaa gccacttctt actaatgctg ggtatctata ttttgaagca aagcttagtt
     4261 cagggttttc aacagtagca attctcgtat ttctatagaa taataattca tcatttctat
     4321 ttttcacttc tccaccatac ttcaagtcat tcgcaataac tgaccagtgt acatgccagt
     4381 tattattttt accgctggca attgtgtcat aattttgctg attataacta atcgttttgg
     4441 agtagctatt tgatgaagtt cgtccaatac ctttcgttga atcgaattta ccacctgagc
     4501 tatatgaaaa tgtactgtct actttcgcag ttgaaatttt atttttcggc aattggtcta
     4561 atatttcagt tttaggattt ctttttactt gaaaatctac atggtactca cttggatact
     4621 ttaaccaatt tgaatttttt tcttctttat gagattcaaa ctttaaattt gaatgaattg
     4681 agccttgttt tttaacaagt aatacattct tgtcataagt tggatcatcg ataaagtcga
     4741 attgtaaatt ttggagtata tttgttttct catcatatac agtttcagtt cgttttgtga
     4801 ttttaccgtt tttcccaata tcatcaggta ctgttgaatt tttatcttta ttagtaacat
     4861 tacgtttctc tttttgttga gacttatcaa catgttcttt cttattttgg tcttgcgagt
     4921 ctttatgagc tgaatttgct tgagtcgttg ctgctgataa caataaaatt gcacatgata
     4981 atgatgacgc tattaaaaca cgttttttat ttttcattgt tataaccttc tttcgtatga
     5041 ttgatatttg ttgatatgta tcgacatgtg aataatatca caaaaataga gaatatatat
     5101 ttaactattt attaaatgat tttgttaata ttattaaata ctttatcctc tttaaaaata
     5161 atgtgtgtac aaagtcatta atttagtaaa tatttttatt tagtagttaa taacc
//
LOCUS       DAMZKC010000158         5126 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.160, whole genome shotgun sequence.
ACCESSION   DAMZKC010000158 DAMZKC010000000
VERSION     DAMZKC010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5126
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.160"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            121..1500
                     /gene="uhpT"
                     /locus_tag="POB55_002200"
     CDS             121..1500
                     /gene="uhpT"
                     /locus_tag="POB55_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HDI6501146.1"
                     /translation="MNFFDIHKIPNKGIPLSVQRKLWLRNFMQAFFVVFFVYMAMYLI
                     RNNFKAAQPFLKEEIGLSTLELGYIGLAFSITYGLGKTLLGYFVDGRNTKRIISFLLI
                     LSAITVLIMGFVLSYFGSVMGLLIVLWGLNGVFQSVGGPASYSTISRWAPRTKRGRYL
                     GFWNTSHNIGGAIAGGVALWGANVFFHGNVIGMFIFPSVIALLIGIATLFIGKDDPEE
                     LGWNRAEEIWEEPVDKENIDSQGMTKWEIFKKYILGNPVIWILCVSNVFVYIVRIGID
                     NWAPLYVSEHLHFSKGDAVNTIFYFEIGALVASLLWGYVSDLLKGRRAIVAIGCMFMI
                     TFVVLFYTNATSVMMVNISLFALGALIFGPQLLIGVSLTGFVPKNAISVANGMTGSFA
                     YLFGDSMAKVGLAAIADPTRNGLNIFGYTLSGWTDVFIVFYVALFLGMILLGIVAFYE
                     EKKIRSLKI"
     gene            complement(1859..2617)
                     /locus_tag="POB55_002201"
     CDS             complement(1859..2617)
                     /locus_tag="POB55_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDI6501147.1"
                     /translation="MFKVVICDDERIIREGLKQIIPWGDYHFNTIYTAKDGVEALSLI
                     QQHQPELVITDIRMPRKNGVDLLNDIAHLDCNVIILSSYDDFEYMKAGIQHHVLDYLL
                     KPVDHAQLEVILGRLVRTLLEQQSQNGRSLASCHDAFQPLLKVEYDDYYVNQIVDQIK
                     QSYQTKVTVSDLIQHIDVSESYAMRTFKDHVGITIVDYLNRYRILQSLQLLDRHYKHY
                     EIADKVGFSEYKMFSYHFKKYLQMSPSDYCKQAK"
     gene            complement(2610..4166)
                     /locus_tag="POB55_002202"
     CDS             complement(2610..4166)
                     /locus_tag="POB55_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDI6501148.1"
                     /translation="MTAYKPYRHQLRRSLFASTIFPVFLVIIIGLVSFYAIYIWIEHR
                     TIHQHVDESQSSLHHTEKQIQTFITQHNNSFQELDLTNHHDVTATKRGLLKLIHQQPA
                     TLYYELSGPNQFITNNYEHLNTKNMYLFSTHQLKFKNSTYMLKIYMANTPRLSEIKKD
                     SRQFALIVDQYDNILYANDDRFTIGEKYRPQQFGFMNESVKLNHADHRLIIYKDIHEN
                     IEDGITLLIVMAVVLVLLVIFGFISADNMAKRQTKDIETIIQKIYYAKNRHLGTYTPL
                     KNNSELEEINNYIYDLFESNEQLIHSIEHTERRLRDIQLKEIERQFQPHFLFNTMQTI
                     QYLITLSPKLAQTVVQQLSQMLRYSLRTNSHTVELNEELNYIEQYVAIQNIRFDDMIK
                     LHIESSEEARHQTIGKMMLQPLIENAIKHGRDTESLDITIRLTLARQNLHVLVCDNGI
                     GMSSSRLQYVRQSLNNDVFDTKHLGLNHLHNKAMIQYGSHARLHIFSKRNQGTLICYK
                     IPLSRGNVDV"
     gene            complement(4163..>5126)
                     /locus_tag="POB55_002203"
     CDS             complement(4163..>5126)
                     /locus_tag="POB55_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDI6501149.1"
                     /translation="SKIYILLLFLIFLSACANTRHSESDKNVLTVYSPYQSNLIRPIL
                     NEFEKQEHVKIEIKHGSTQVLLSNLHNEDFSERGDVFMGGVLSETIDHPEDFVPYQDT
                     SVTQQLEDYRSNNKYVTSFLLMPTVIVVNSDLQGDIKIRGYQDLLQPILKGKIAYSNP
                     NTTTTGYQHMRAIYSMHHRVSDVHQFQNHAMQLSKTSKVIEDVAKGKYYAGLSYEQDA
                     RTWKNKGYPVSIVYPIEGTMLNVDGIALVKNAHPHPKRKKLVQYLTSRSVQQRLVAEF
                     DAKSIRKDVSEQSDQSIENLKNIPLIPKSKLPDIPHHKFLEMIQ"
BASE COUNT         1563 a          804 c          948 g         1811 t
ORIGIN      
        1 atatgtttct aagtatgtgt ttatgttcag tattttggat aatttaataa ttttaaggat
       61 attaagcgct tacaccgacg tgatatattt ggcttaacga aaatgattga ggtgacagag
      121 atgaactttt ttgatatcca taagattccg aacaaaggca ttccattatc ggtacaacgt
      181 aaattatggc ttagaaactt catgcaagct ttctttgtag tgttctttgt ttatatggct
      241 atgtatttaa ttcgaaacaa ctttaaggcg gcccaaccgt ttttaaaaga ggaaattgga
      301 ttatctacat tagaacttgg ttatatcgga ttagcattta gtatcacgta cggtttaggg
      361 aaaacattac ttggatattt tgtcgatgga cgtaacacaa aacgtattat ctcgttctta
      421 cttatcttat ctgcgattac agttttaatt atgggatttg ttttaagtta ctttggttct
      481 gtaatgggat tattaattgt actttgggga cttaacgggg tgttccaatc agttggtgga
      541 cctgcaagtt attcaacgat ttcaagatgg gcgccaagaa cgaaacgtgg ccgatactta
      601 ggattttgga atacatcaca taatatcggt ggtgccattg caggtggtgt tgcactttgg
      661 ggtgctaatg tattcttcca tggaaatgtt atagggatgt tcattttccc atcggtgatt
      721 gcattactta ttggtatcgc aacattattt atcggaaaag atgatccaga agaattagga
      781 tggaatcgtg ctgaagaaat ttgggaagag ccggtcgata aagaaaatat tgattctcaa
      841 ggtatgacga aatgggagat ctttaaaaaa tatatcctgg gaaatcctgt tatatggatt
      901 ctatgtgttt caaacgtctt tgtatacatt gtacgaatcg gtattgataa ctgggcaccg
      961 ttatatgtgt cagagcattt acactttagt aaaggcgatg cagttaatac gatattctac
     1021 tttgaaattg gtgcattagt tgcaagttta ttatggggct acgtatcaga cttattaaaa
     1081 ggtcgtcgtg caattgtagc tattggctgt atgtttatga ttacatttgt tgtcttattc
     1141 tacacaaatg ctacaagtgt catgatggtt aacatttcat tgtttgcatt aggtgcgtta
     1201 atctttggtc cgcaattatt aattggtgta tcattgactg gttttgttcc taaaaatgcc
     1261 atcagtgtag caaacggaat gacaggttca ttcgcgtatc tattcggtga ctcaatggcg
     1321 aaagttggtt tggcggctat tgctgatcca acacgtaacg gtttaaacat ctttggatat
     1381 acattaagtg gatggacaga tgttttcatc gtcttctatg ttgcattatt cctaggcatg
     1441 attctattag gaatcgttgc tttctatgaa gaaaagaaaa ttagaagttt aaaaatttaa
     1501 tataaatcgg attaaaagta tcgccaatct attgcaatat agttggcaat cctgccccga
     1561 cggcatgtgc gtgaagagat gaaagatact gcttctaccc ttgcaaatat atcatctcta
     1621 tgtctcgggg cagatcataa ttccctgtta tgaagtatcc ttatttgccc gacttagggt
     1681 gactcaatga atttactcct tacaataaag acatatagcg gtgtcaatat tgtagggagt
     1741 attgttttat atttaaactc tctaaaaagc ggactgaaag aaaagtgaaa acttctctat
     1801 cagtccgctt tttcatagaa caaaatgtag gcgccataat cattagttat gtgctaatct
     1861 attttgcttg cttacaataa tcacttggcg acatttgtaa atatttttta aaatgatagc
     1921 taaacatttt atactctgaa aagcctactt tgtctgcaat ttcatagtgt ttgtaatgtc
     1981 gatctaacaa ttgcagagat tgtaaaatac gatagcgatt taaataatcg acaattgtaa
     2041 taccaacatg atctttaaat gttcgcatcg catacgattc actaacatcg atatgttgaa
     2101 ttaaatctga aacagtcact ttcgtttgat aagattgctt aatttgatca acaatttgat
     2161 ttacataata atcatcgtat tctactttta ataatggttg gaaggcatca tgacaagatg
     2221 ctaagctacg gccgttctgt gattgttgct ctaataaggt acggacaagt cttcctaaaa
     2281 taacttctaa ttgtgcatgg tccactggtt ttaataaata atcaagaaca tgatgttgaa
     2341 taccggcttt catatattca aagtcatcgt aactcgataa tatgatgaca ttacaatcta
     2401 gatgcgcaat atcattgagt aaatcgacgc catttttacg tggcatacga atatcagtaa
     2461 ttactaattc tggctgatgt tgttgaatta gtgataatgc ttcaacacca tctttagcag
     2521 tgtatattgt attgaaatga tagtctcccc aaggaatgat ttgctttaaa ccttctcgaa
     2581 taattcgttc atcatcacaa ataactacct taaacatcta cattccccct tgaaagtggt
     2641 attttataac aaattaacgt accttgatta cgctttgaaa aaatatggag tcgtgcatgt
     2701 gaaccatatt gaatcattgc tttattgtgt aaatgattta atcccaaatg cttagtatca
     2761 aatacatcat tattaagaga ttggcgtaca tattgcaggc gagatgacga catcccaata
     2821 ccattgtcgc aaactaaaac atgtaaattc tgacgtgcca atgtcaggcg tatagtaata
     2881 tccaatgact cagtatctct accatgttta atagcatttt ctatgagtgg ttgaagcatc
     2941 attttaccaa ttgtctggtg acgcgcttct tcagaacttt cgatatggag cttaatcatg
     3001 tcatcaaaac ggatgttttg tatcgcaaca tactgttcaa tgtagttcaa ctcttcgttt
     3061 aattccactg tatgtgagtt tgtacgtaat gagtaacgta acatttgcga taattgttgg
     3121 accacagttt gtgctaattt cggagataac gtaattaaat attgtattgt ttgcatcgta
     3181 ttgaatagga aatgaggttg gaattggcgt tctatttcct ttaactgaat atcacgcaag
     3241 cgacgttctg tatgctcgat agaatggatc agttgctcat ttgattcaaa taaatcgtaa
     3301 atataattat taatttcttc tagttcactg ttgtttttta aaggtgtata tgtacctaga
     3361 tgacgatttt tggcatagta aattttttga ataatcgttt cgatatcttt tgtttgtcgt
     3421 ttagccatat tatctgcgct aatgaaacca aatattacta gtaaaacaag aactacggcc
     3481 ataacaatta acaacgtgat accatcttca atgttttcat gtatatcttt ataaataatg
     3541 agacgatggt cagcatggtt taattttaca gattcattca taaatccgaa ttgttgtggt
     3601 ctatactttt cacctatagt aaaacggtca tcgttggcgt ataaaatatt gtcatattga
     3661 tcaacgataa gtgcgaattg tcggctatct ttcttaattt cacttaaacg tggggtgtta
     3721 gccatataaa ttttaagcat atatgtacta tttttgaatt taagctgatg cgttgaaaat
     3781 aaatacatat ttttagtgtt taaatgttca taattattgg ttataaactg atttggtcca
     3841 gataattcat aataaagtgt tgcgggctgt tggtgtatta attttaataa tccacgtttt
     3901 gtagcggtaa catcatgatg atttgttaaa tcgagctctt gaaacgaatt attatgctgt
     3961 gtaataaatg tctgaatctg cttttcagtg tgatgtaaag atgactgact ttcatcaaca
     4021 tgttgatgaa tcgtacgatg ctcaatccaa atatagatgg catagaagct tactagtcca
     4081 ataataatga ctaaaaatac cggaaaaata gtagacgcaa ataacgatcg tctcaattga
     4141 tgtctataag gtttgtatgc cgtcattgaa tcatctccaa aaatttatga tgtggaatgt
     4201 ccggtaattt agatttcggt attaaaggta tgttcttaag attttcgata gactgatcgc
     4261 tttgttcact aacatccttt cgaattgact tggcatcgaa ctctgcaact aatcgttgtt
     4321 gtactgagcg gcttgttaaa tattgcacta actttttacg cttaggatga gggtgtgcat
     4381 ttttaactaa agcaatacca tcaacattta acattgttcc ttcaattgga taaacgattg
     4441 atacaggata acctttgttt ttccatgtgc gtgcatcttg ttcgtagctt agacctgcgt
     4501 aatatttacc ttttgcaaca tcttcaatga ctttagacgt ctttgacagt tgcatcgcat
     4561 ggttttggaa ttgatgcaca tcacttactc gatgatgcat gctataaata gcgcgcatat
     4621 gttgatagcc tgtcgttgtt gtatttggat ttgagtacgc aattttacct ttaagtatag
     4681 gttgtaataa atcttgataa cctcgaatct taatatctcc ttgtaaatct gaattcacta
     4741 ctataactgt tggcattaat agaaaactag taacatattt attgttcgag cgataatcct
     4801 ctaattgctg tgttacagat gtatcttgat agggaacaaa atcttctgga tgatcaattg
     4861 tttctgataa cacaccaccc ataaagacgt caccacgctc cgaaaaatct tcgttatgca
     4921 agtttgaaag cagtacttga gtagatccat gtttgatttc aattttgaca tgctcttgtt
     4981 tttcaaattc atttaaaatt ggacgaatca agtttgattg atacggagaa taaactgtta
     5041 atacattttt atctgattca gagtgacgcg tattagcgca tgctgataaa aaaatgagaa
     5101 ataatagcaa gatataaatt tttgat
//
LOCUS       DAMZKC010000159         5073 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.161, whole genome shotgun sequence.
ACCESSION   DAMZKC010000159 DAMZKC010000000
VERSION     DAMZKC010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5073
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.161"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            197..1654
                     /locus_tag="POB55_002204"
     CDS             197..1654
                     /locus_tag="POB55_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500488.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDI6501150.1"
                     /translation="MKCLIRFILVLGLLISSAMVYINPTAHAEQDQTWEKIKERGELR
                     VGLSADYAPMEFEHTVNGKTEYAGVDIDLAKKIAKDNNLKLKIVNMSFDSLLGALKTG
                     KIDIIISGMTSTPERKKQVDFSDSYMMTKNIMLVKKDKVNEYKDIKDFNNKKVGAQKG
                     TEQEKIAQTEIENASITSLSRLPDVILALKSGKVEGAVVEKPVAEAYLKQNPKLGISN
                     VKFNEEEKDTVIAVPKDSPKLLSQINKTIKEVKDKGLIDKYMTNAANAMNDDSGFISK
                     YGSFFLKGIKITILISLIGVALGSILGAFVALMKLSKIKIISWIASIYIEILRGTPML
                     VQVFIVFFGITAALGLDISALVCGTIALVINSSAYIAEIIRAGINAVDKGQMEAARSL
                     GLNYRQTMKSVIMPQAIKNILPALGNEFVTLIKESSIVSTIGVGEIMFNAQVVQGISF
                     DPFTPLIVAAALYFVLTFVLTRIMNMIEGRLNASD"
     gene            1647..2369
                     /locus_tag="POB55_002205"
     CDS             1647..2369
                     /locus_tag="POB55_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDI6501151.1"
                     /translation="MIKINNLNKVFGDNEVLKDINLEINQGEVVAIIGPSGSGKSTLL
                     RCMNLLEVPTKGQVIFEGNDLTEKGTQVDKLRQKMGMVFQNFNLFPHKKVVDNIILAP
                     KLLKKDNNDELHKEALSLLDKVGLKEKADVYPNQLSGGQKQRVAIARALAMHPDVILF
                     DEPTSALDPEVVGDVLKVMKDLAKEGMTMVVVTHEMGFAKDVSDKVIFMADGVVVESG
                     TPVEIFEQPQHERTQNFLARVL"
     gene            2520..3647
                     /gene="queG"
                     /locus_tag="POB55_002206"
     CDS             2520..3647
                     /gene="queG"
                     /locus_tag="POB55_002206"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HDI6501152.1"
                     /translation="MDTKQLKQDIIDYAYTIGIDSIGFTTADPFDELKQKLEAYHANG
                     YASGFEESDIALRTEPKLSLPTARSIIAIAVGYPNKLKGAPKSVRGDRRGLFARASWG
                     QDYHTIMRKRLDMLASFIESKVPDVEIKSMVDTGVLSDRAVAERAGLGFVGRNGFVIN
                     PKLGTWTYLGEMLVSIPFEPDDPLLDSCGDCTICVDRCPTSALVGNGQLNSQKCISFL
                     TQTKGYMPDQYRYKIGNRLYGCDTCQQVCPKNRGINTEQDDIILEPEILKPRLVPLLR
                     MSNKEFKQTYGHLAGAWRGKKPIQRNAILGLAHFNEVDAIPELKKVATTDERPMIRAT
                     AYWAIGQILGEEARDFINANYDQEDAEVQNEMIKGLDTRRE"
     gene            3652..4122
                     /gene="trmL"
                     /locus_tag="POB55_002207"
     CDS             3652..4122
                     /gene="trmL"
                     /locus_tag="POB55_002207"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HDI6501153.1"
                     /translation="MTNHIVLYQPEIPANTGNIARTCAGTNTHLHLIKPLGFRTDDKM
                     LKRAGLDYWEFVNITYHDSIEAFFEATNGEYYLLTKFGKKTYSDFDFSNHDKDFYFIF
                     GKETTGLPDWVKEKYQDTALRIPMSEHIRSLNLSNTAALLIYEALRQQDFPGLN"
     gene            4281..4880
                     /locus_tag="POB55_002208"
     CDS             4281..4880
                     /locus_tag="POB55_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate isomerase"
                     /protein_id="HDI6501154.1"
                     /translation="MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAP
                     VLDELKKNVEKHAVDFSQINILDYDDKKSYFEALGVPAGQVYPIAYEKDAIELIADKI
                     KTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
                     TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA"
     gene            4905..5057
                     /locus_tag="POB55_002209"
     CDS             4905..5057
                     /locus_tag="POB55_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501155.1"
                     /translation="MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKN
                     SEEDSQ"
BASE COUNT         1889 a          652 c          976 g         1556 t
ORIGIN      
        1 aagatattga tagtagcgct tttttgattg attttaaaat gtgagatttg ttcacgtata
       61 atagcggatt ttttatgtac agtcttttaa aaagaagatt ttctttatat taataaaaat
      121 acatttataa aacactattg catcaaaatg tattaatatg tataattatg aatattaatt
      181 agggagggct gtataaatga agtgtttaat taggtttata ttagtattag ggttgttaat
      241 tagcagtgct atggtgtata tcaatcccac agcacatgct gaacaagatc aaacatggga
      301 gaagattaaa gaacgcggtg aacttagagt gggtctttct gcagattatg caccaatgga
      361 atttgagcat acagttaatg gtaagactga gtatgcaggt gtagatattg atttagctaa
      421 aaaaattgcg aaagataata atttaaaatt aaaaatcgtc aatatgtcat ttgatagttt
      481 gttaggagct cttaaaactg gaaaaattga tattattatt tccggaatga cttcaacgcc
      541 tgaacgtaag aagcaagttg atttttcaga ttcatatatg atgactaaaa atatcatgct
      601 tgtaaagaaa gataaagtta atgaatataa agatatcaaa gactttaata ataaaaaagt
      661 aggggcacaa aagggaactg aacaagaaaa aatcgctcaa accgaaattg aaaatgcatc
      721 tattacttca ttaagccgtt taccagacgt tatattagca cttaaaagtg gaaaggttga
      781 aggtgctgta gttgaaaaac ctgtagcaga agcatattta aaacaaaatc ctaaattagg
      841 aatttccaat gtgaaattta atgaagaaga aaaagataca gtgatagcag tgccaaaaga
      901 ttcaccaaaa ttattgtcac aaattaataa aacgattaag gaggttaaag ataaaggatt
      961 aatcgataaa tatatgacta atgctgcaaa tgcgatgaat gatgacagtg gttttatttc
     1021 taagtatggt agttttttct tgaaaggaat taagattaca atattaattt cacttatcgg
     1081 tgttgcatta ggttctattt taggtgcatt cgttgcgtta atgaaattaa gtaaaataaa
     1141 aattatttca tggattgctt ctatctatat cgaaatatta agaggaacac caatgttagt
     1201 tcaagtattt atcgtattct ttggtataac tgctgcatta ggactagaca tttcagctct
     1261 tgtttgtgga acaattgcat tagttattaa ttcctcagct tacattgctg aaattattcg
     1321 tgcaggtata aatgctgttg ataaaggcca aatggaagcc gcacgtagtc taggtttgaa
     1381 ttatagacaa acgatgaaaa gtgtaattat gccacaagca attaaaaata ttttaccagc
     1441 tttaggtaat gaatttgtca ctttaattaa agaatcatct attgtgtcaa caattggagt
     1501 tggcgaaatt atgtttaatg cacaagtggt tcaaggtatt tcatttgacc cattcacacc
     1561 attaatagtg gcagcagcat tatactttgt cttaacattt gtacttacac gcattatgaa
     1621 catgattgaa gggagattga atgccagtga ttaaaataaa caatcttaat aaagtttttg
     1681 gagataatga agttttaaaa gatatcaatc ttgaaatcaa tcaaggggaa gtagtagcaa
     1741 taataggtcc atctggtagt ggtaaaagta cattgttaag atgtatgaat ttattagaag
     1801 tacccactaa aggtcaagtg atttttgaag gcaatgactt aacggaaaaa gggacacaag
     1861 tagataaact acgtcaaaaa atgggtatgg tatttcaaaa cttcaaccta tttccacata
     1921 aaaaagttgt cgataatatt attttagctc ctaaattatt aaagaaagat aataacgatg
     1981 aattacataa ggaagcattg tcgttattag ataaagtggg attaaaagaa aaagcagatg
     2041 tatatccgaa tcaattatca ggtggtcaaa agcaaagggt agcaattgca agagctttag
     2101 caatgcatcc agatgttatt ttattcgatg aaccaacttc agcattagat cctgaggtag
     2161 ttggtgatgt attaaaagta atgaaagacc tagccaaaga aggtatgacc atggtggttg
     2221 tgacacatga aatgggattt gccaaagatg taagtgacaa agtcatattt atggcagatg
     2281 gcgttgtcgt agagtcaggc acaccagtcg aaatatttga acaaccgcaa catgaaagaa
     2341 cacaaaattt cttagcaaga gtattataac aacctaacga agcttgaata tatgatacgc
     2401 accacaaagt tatatcatat attcaagctt tttattatta aaattatgtg aaatgaaacc
     2461 tcaatatgaa taatggttgt gttaaaataa catagtatta tttaatgggg ggcatacgat
     2521 tggatacaaa gcagttaaag caagatatca ttgactatgc atatacaatt ggtatcgaca
     2581 gtattggatt tacaactgcc gatccctttg atgaattgaa gcaaaagcta gaagcatatc
     2641 atgcaaatgg ttatgcctca ggatttgaag aatctgatat tgctttacga acggagccta
     2701 aattatcctt accaacagca aggtcaatca tagcaattgc agttggttat cctaacaaac
     2761 tgaaaggtgc acctaagagt gttagaggtg atcgcagagg cttatttgct agagcatcgt
     2821 ggggtcaaga ttatcataca attatgcgta aacgattaga catgttagct tcatttattg
     2881 aatctaaagt tccagatgtt gaaataaaat ctatggtaga tacgggtgta ttatcagata
     2941 gggcagtagc agaacgtgca ggtttaggat ttgttggtcg taatggcttt gtcatcaatc
     3001 ctaaactagg aacatggaca tacctcggtg aaatgttagt cagtatacct tttgaacctg
     3061 atgatccatt attagatagc tgtggtgatt gtacaatttg tgttgatcgt tgtccaacaa
     3121 gtgcattagt tggaaatggc caattaaata gtcagaaatg cattagtttt ttaacgcaaa
     3181 caaaaggcta tatgcctgac caatatcgtt ataaaattgg gaatagatta tatggttgcg
     3241 atacgtgtca acaagtttgt ccgaaaaata gaggtattaa taccgaacaa gatgacatca
     3301 ttttggaacc agaaatttta aagccaagat tagtaccttt actacgtatg tctaataaag
     3361 aattcaaaca aacatatggt caccttgcag gtgcttggcg tggtaaaaaa ccgatacaac
     3421 gaaatgctat tttaggattg gcgcatttta atgaagtgga tgcaattcca gaattgaaaa
     3481 aagttgcaac aacagatgaa agaccgatga ttcgagcaac tgcttattgg gccattggtc
     3541 aaattcttgg ggaagaagca agagatttta tcaatgcaaa ttatgatcaa gaggatgcag
     3601 aagttcaaaa tgaaatgata aaaggattag acacaaggag agaatagtaa tatgacaaat
     3661 catatcgttt tatatcaacc agaaatccca gctaatactg gtaacattgc acgtacgtgt
     3721 gcaggtacaa acacacattt acatttaatt aaacctcttg gatttagaac tgatgataaa
     3781 atgttaaaaa gagccggttt agattattgg gaatttgtga atattacgta tcatgatagt
     3841 attgaagcat tttttgaagc cacaaatggc gagtattact tattaacaaa atttggtaaa
     3901 aagacttata gtgattttga tttttcaaat catgacaaag atttttactt catttttgga
     3961 aaagagacga caggattacc agactgggtg aaagagaaat atcaagacac agcattaaga
     4021 attcctatga gtgaacatat tcgttcatta aatttatcaa atactgctgc gttgttaatt
     4081 tatgaagcgt tacgtcaaca agattttccg ggattgaatt aaaatttact cattttcatt
     4141 ataaaaatga tgcttaagtg ctatatttag agtataagca tgttaaagtt aaatggtttg
     4201 atatattgaa agttagggta taataattat taatatcgcc gtttttatgt aaaatgcaaa
     4261 ctaagaggag tgtagaaatc atggcaatga actttaaagt ctttgacaat agtcaacttg
     4321 tagcagaata tgctgctgat attattagaa agcaatttaa caataatcct actacaattg
     4381 caggttttca tttagataca gatcaagcgc cagttctaga tgaattaaag aaaaatgttg
     4441 aaaaacatgc tgttgatttt agccaaataa atattttaga ttatgacgat aaaaaatcat
     4501 atttcgaagc gttaggtgta ccagcaggtc aagtttatcc aattgcttat gaaaaagatg
     4561 caatcgaatt aatcgctgat aagattaaaa ctaaagaaaa taaagggaaa ttaacattac
     4621 aagttgtttc tatcgatgag caaggtaagt taaatgttag tattcgtcaa ggactaatgg
     4681 aagcaagaga aattttctta gtagtgacag gtgctaataa acgagatgta gttgaaaaat
     4741 tatatcaaga aaatggtaaa acaagcttcg aaccagccga tttaaaagca catagaatgg
     4801 taaatgttat tcttgataaa gaagcggctg caggtttacc tgaagatgtt aaagcttact
     4861 ttacgtcacg ctttgcttaa ttaaaaatct gtgaggtatt gattatgtct aaagacaaag
     4921 atccaaaatt aaattatcat gaagaagaaa acagtatggt aacggatttt gaagatttaa
     4981 aagaattagg taaagaaatg gaacaaatct ctgatcaaaa tgatcaagaa aagaattctg
     5041 aagaagacag tcagtaaagt attataattg tgt
//
LOCUS       DAMZKC010000160         5071 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.162, whole genome shotgun sequence.
ACCESSION   DAMZKC010000160 DAMZKC010000000
VERSION     DAMZKC010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5071
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.162"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(<1..948)
                     /locus_tag="POB55_002210"
     CDS             complement(<1..948)
                     /locus_tag="POB55_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501156.1"
                     /translation="MIKNKWKIEMWFRNFSYFIHKIDSEFILEEYLEDYLPKEYRPNF
                     ICGKAQPSETIYVSYFINQDEQTVEQHTVECDKTGFFIDEIPKHFIKNKMHYFIDSQS
                     KIDIKDLLNEFIDQGTAILNILDLNDLETYFNDYLETINHTTEFAKKIANKAMKEIRK
                     NIELEQIKQQVSYMKNADQVSSLESFKDLKHTILKQHRDLSLKNVDDIIISDFLTRYL
                     IYKEKQDSYVKKDISSINKSQYFKKLVIEGLIRERKSNSQYFEIANSIKPSSHNNNSL
                     NQSDEIDQFRQAVIGISLNLYDHNDTYFKFIRSIMKKSSR"
     gene            1093..1797
                     /locus_tag="POB55_002211"
     CDS             1093..1797
                     /locus_tag="POB55_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016170663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FRG domain-containing protein"
                     /protein_id="HDI6501157.1"
                     /translation="MKRSVESFSELHQVLKNYRDNSLWLFRGHSNLNWSLVPKAGRAP
                     YDKYDDEETFYAWKRQAMIFLKETYSDWDMLTIAQHHGLATRLLDWSFNPLVAAFFAV
                     QTYEDCDAVIYAYLKKSDMNMKGATPFKSKWIKKVKPNGLASRVVRQSGIFTIHEPPT
                     NSIEANLSEKEELHKIIIKKHYRKKLREELSYYGINELSLFPDLDGLSSHVNWIMEIN
                     PYASNNITQELNSINE"
     gene            1821..2663
                     /locus_tag="POB55_002212"
     CDS             1821..2663
                     /locus_tag="POB55_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HDI6501158.1"
                     /translation="MSVLETKLKSQMSKSAKIARNMNKLPDEIDRLRKRIERINKKRK
                     PTSSNIRDLEKSNKQLVTKQQKLADLQVEYTKIEKKINETKINLQKEQSRNQKKLSSM
                     LDKNTKGNEEIMEKLLTNSDQINEISNQIKKAVNQKEIIEYDVFLSHSSLDKEDYVSK
                     ISEKLIEKGLKVFEDVKVFEIGKSQTETMNMGILNSRFVVVFLSPNFIESGWSRYEFL
                     SFLNREINEEHVIILPIWHKVSVEDVRAYNPYLVDKYALNTSDFSIEEIVEKIYQVIV
                     NSKN"
     gene            3137..3415
                     /locus_tag="POB55_002213"
     CDS             3137..3415
                     /locus_tag="POB55_002213"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501159.1"
                     /translation="MKTVYPVGTELSIDEYEIVKNKIMVLGKEKWTNLLNEPHYYYLI
                     EDFIETDYKKTSKGGLMGVKYFNVNEILNRDCLTTEQIAKELCNKDWE"
     gene            3525..3683
                     /locus_tag="POB55_002214"
     CDS             3525..3683
                     /locus_tag="POB55_002214"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501160.1"
                     /translation="MYLKGVEGDTPKYAVPILETDFSDLPSTHTTVGSLDRFRDKTMS
                     INRSGSNC"
     gene            4001..4639
                     /locus_tag="POB55_002215"
     CDS             4001..4639
                     /locus_tag="POB55_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fusidic acid resistance EF-G-binding protein
                     FusC"
                     /protein_id="HDI6501161.1"
                     /translation="MNKIEVYKFVKVKQLVYQLIKLYRTNDMNSHKTQKDFLLNEIND
                     IFKEKDIDISDFITSIDDVKLTKKKAEHLLNELKVYIQDFEIPSSSQLEKIFRKVKKL
                     KRPDINLIDTKEISYLGWNDNSSNRKYIVYKNLDDKFEGIYGEISPNKVKGFCKICNQ
                     ESDTSLFLNKTKHNKSSGTYTKKGDYICYDSFKCNQNLDDINNLYEFIVKIK"
BASE COUNT         1982 a          597 c          820 g         1672 t
ORIGIN      
        1 acgtgaactt tttttcataa tactacggat aaatttgaag taagtatcat tatgatcgta
       61 tagattaaga gaaataccaa ttacagcctg tctaaattga tctatttcat cagattgatt
      121 taagctatta ttattgtgag atgaaggttt gatactatta gctatttcaa agtattgaga
      181 gttactcttt ctttctctaa ttaaaccttc gattactaac tttttaaaat actgagattt
      241 atttatactt gagatatctt tttttacata cgaatcttgt ttttctttat aaatgaggta
      301 tcttgttaaa aagtcgctta ttataatatc atcaacattt tttaacgata aatctcggtg
      361 ttgttttaat atcgtatgct ttaaatcctt aaagctttct aaagaagaga cttgatctgc
      421 atttttcata taacttactt gctgtttaat ttgctctaac tcaatatttt ttcgaatttc
      481 tttcatagct ttgttagcta tttttttagc gaattctgtt gtatggttga ttgtttctaa
      541 gtaatcatta aaatatgttt ctaggtcatt taaatctaat atattcaaaa tggctgtacc
      601 ttgatcaata aactcattta ataagtcttt aatatcaatt ttactttgac tatcaataaa
      661 ataatgcatt ttatttttta taaaatgttt agggatttca tctataaaaa aaccagtttt
      721 gtcacattcc acagtatgtt gctccacagt ttgttcatcc tgatttataa aataacttac
      781 atatatagtt tcgctaggtt gggcttttcc acatataaaa tttggtcggt attctttagg
      841 taaataatcc tctaagtatt cttctaatat aaattctgaa tcaattttat gtataaaata
      901 actaaaattc cgaaaccaca tttctatttt ccatttattt ttaatcatag tattactcac
      961 tttcaataat taaatgtata gacataatgc tagttttatt tttaagatat ttcaataatg
     1021 tttactaatt tttccctata agataagata tacaatataa aagggaacaa acgttcctat
     1081 tggaggggtc ctatgaaaag gtcagtcgag tctttcagtg agttacatca agttttaaag
     1141 aattatagag ataatagctt atggttattt agaggtcata gtaatttgaa ttggagttta
     1201 gttccgaaag ctggtcgagc accttatgat aaatatgatg atgaagaaac attttatgct
     1261 tggaaacgtc aagcaatgat atttttaaaa gagacttata gtgattggga tatgctaact
     1321 atagcgcaac atcacggatt agctactaga ctattggatt ggagttttaa tcctttagtt
     1381 gctgcctttt ttgcggtgca aacatatgag gattgtgatg ctgttattta tgcctattta
     1441 aaaaaatcgg atatgaatat gaaaggggct acgcctttta agagtaaatg gataaaaaaa
     1501 gtaaagccta acggtctagc ttcgagagtg gttagacaaa gtggaatttt tactattcat
     1561 gagcctccta caaattctat agaagctaat ctttcagaga aagaagaact acacaaaatc
     1621 atcataaaga aacattatcg caaaaaatta agagaagagt tatcttatta tggtatcaac
     1681 gaattatcat tatttcctga cttagatggt ttatctagcc atgttaattg gataatggag
     1741 ataaatccat acgcttcaaa taatataacg caagaattaa attcaattaa tgaataagat
     1801 tataaaaagc gaggtattat atgtcagtat tagaaactaa attaaaaagt caaatgtcaa
     1861 aatctgcaaa aatagctaga aatatgaata agttaccaga tgaaattgat agattaagaa
     1921 aaaggataga aagaataaat aaaaaaagaa aacctacttc cagtaatatt agagatttgg
     1981 aaaaatctaa taagcaatta gttacaaagc agcaaaaact tgcagattta caagttgagt
     2041 atactaagat tgagaaaaaa attaatgaaa caaaaattaa tcttcaaaaa gagcagtcta
     2101 ggaatcaaaa gaaattatcc agtatgttag ataaaaatac aaaaggaaat gaagagatta
     2161 tggaaaaatt attaactaat tcagatcaaa taaatgagat atcaaatcag ataaaaaaag
     2221 cagtgaatca aaaagagata attgaatatg atgtctttct atcccattct agtttagata
     2281 aagaagatta tgtatctaag ataagtgaaa aactaataga aaaaggacta aaagtctttg
     2341 aagatgttaa agtttttgaa atcggaaaaa gtcaaacaga aactatgaat atgggtatac
     2401 taaactctcg gtttgttgta gtatttttat cccctaattt tattgaaagt ggttggagca
     2461 ggtacgaatt tttatccttt ctaaacagag aaataaatga agagcatgtt attatattac
     2521 ctatttggca taaagtgagt gttgaagatg tacgtgcata caacccatat ttagtagata
     2581 agtatgcatt gaatacaagt gattttagta tagaagaaat agtagaaaaa atttatcaag
     2641 taatcgttaa ttctaagaat tagtaatagt attggttatc tactttaaaa gcaaagaggg
     2701 aattaaatga aaaaagcagt gtttttatta ctaaatgagt atgcagaatg gtaagcaagc
     2761 tactgttcct ctattttaaa ccaaagcagt gaatggaaaa tagaaacaac ttcaatttat
     2821 gaatatttta cctcgattgg tggatttaca attaaagtag taatagttat ccaatatttt
     2881 ctagttgatt ctcttataat atgtccaatt aagtgtagat gattcaagat gatattgagg
     2941 gaaggatgag aatgtgtctt tcaggtagct catttgatga taaccttaaa cataatattt
     3001 actttcatcc tgctgaaaaa gaggttacag tttcaagccg atagaataca ttgggttata
     3061 taaagacaaa gcaataaaag ctatcggaaa agtagacaaa gtaatagtga cggaaataaa
     3121 tgagagctca ttatccttga agacagttta tcctgtaggt actgagttat cgattgatga
     3181 gtacgaaata gttaaaaata aaataatggt gcttgggaaa gaaaagtgga ctaatctatt
     3241 gaacgaaccc cattattatt acttaattga agattttata gagacagact acaagaaaac
     3301 atctaaaggt ggattgatgg gagttaagta ttttaatgtg aatgaaattt tgaataggga
     3361 ttgcttaacc accgaacaaa tcgctaaaga attatgtaat aaagattggg aatgataaat
     3421 agtgaatgaa gctataatgc aataatgtat gtataacata atcaagtaat gaatttaatg
     3481 ataagagagt atggagtaaa gaattgaatt agtttgcatg gtctatgtat cttaaaggtg
     3541 ttgaaggaga tacaccgaag tatgcagtac caattcttga aacagacttt agtgatttac
     3601 catctacaca tacgacagtc ggatcacttg atcggtttcg agataagaca atgagcatta
     3661 acagaagcgg gagtaactgt tgaattccat ttatatccag ttagatacta gagtttgaag
     3721 tgataaatct tgattcggat gctgaaataa gtaaaagagc agttactgaa tataatataa
     3781 gtataaatat gccactagat attttaagga gtatttattc acttttataa aatcatctgc
     3841 taagatagta tggaagattt ttataggagc tgattataat gcgacatata aatatgtcat
     3901 acatcatacg ctatagtact ctaactcaaa gataagaagt acttagttat gaatagcatt
     3961 catttcacaa agtacttcaa caaaaatgga ggaatatgaa atgaataaaa tagaagtgta
     4021 taagtttgtt aaagtaaagc agttagtata tcaattgatt aagttatatc gtacaaacga
     4081 tatgaattcc cataaaacac aaaaagattt tttactaaat gaaattaatg atatctttaa
     4141 agaaaaagat attgatatct cggactttat tacatcgatt gacgatgtaa aattaactaa
     4201 gaaaaaagca gaacatcttt taaatgaatt aaaagtgtac atccaagatt ttgaaatacc
     4261 ttcatcaagt caactggaga aaatttttcg taaagtaaaa aaattaaaga gaccagatat
     4321 aaatttaatt gatacaaaag aaatttcata tttaggatgg aatgataatt cttctaaccg
     4381 aaaatatatc gtttataaaa atttagatga taaattcgaa ggtatatatg gcgaaatttc
     4441 accaaataaa gtaaaaggat tctgtaaaat ttgtaatcag gaatctgata catcactctt
     4501 tctcaataaa actaaacata ataagagtag tggaacatat actaaaaaag gagattacat
     4561 ttgttatgac agttttaaat gtaatcagaa cctagatgat ataaataatc tttacgaatt
     4621 tattgttaaa ataaaataga tccaaacagc cctgatcttt agaactaatg atcagggttg
     4681 ttatattttg tacctcactc ttaatcgata cgctgaaagt ccctataaat agaacgtacg
     4741 ttccgtttgt agtataatga caaaaatatt aaattagagg attaaaaatg ggattataat
     4801 tttttaatgt accaaactga aaaaggctaa ataaagataa atataagatt agaaaaaact
     4861 gtatggatta ggcaatattt atatataatg aaatgaatta acagaaaatc gaattatccg
     4921 gaaaaaccgt actgttcgat tggaagggaa tagagaaggt gaaaagaatt aacttcaatt
     4981 atcatcttat ttctgaaaaa agatatgaaa aacggacgca agacacttta aatattagta
     5041 acaacgtaaa gtgaataaaa tctatattaa g
//
LOCUS       DAMZKC010000161         4968 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.163, whole genome shotgun sequence.
ACCESSION   DAMZKC010000161 DAMZKC010000000
VERSION     DAMZKC010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4968
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.163"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            43..1503
                     /gene="ebpS"
                     /locus_tag="POB55_002216"
     CDS             43..1503
                     /gene="ebpS"
                     /locus_tag="POB55_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elastin-binding protein EbpS"
                     /protein_id="HDI6501162.1"
                     /translation="MSNNFKDDFEKNRQSIDTNSHQDHTEDVEKDQSELEHQDTIENT
                     EQQFPPRNAQRRKRRRDLATNHNKQVHNESQTSEDNVQNEAGTIDDRQVESSHSTESQ
                     EPSHQDSTPQHEEEYYNKNAFAMDKSHPEPIEDNDKHETIKEAENNTEHSTVSDKSEA
                     EQSQQPKPYFATGANQANTSKDKHDDVTVKQDKDESKDHHSGKKGAAIGAGTAGVAGA
                     AGAMGVSKAKKHSNDAQNKSNSDKSNNSTEDKVSQDKSKDHHNGKKGAAIGAGTAGLA
                     GGAASKSASAASKPHASNNASQNHDEHDNHDRDKERKKGGMAKVLLPLIAAVLIIGAL
                     AIFGGMALNNHNNGTKENKIANTNKNNADESKDKDTSKDASKDKSKSTDSDKSKEDQD
                     KATKDESDNDQNNANQANNQAQNNQNQQQANQNQQQQQQRQGGGQRHTVNGQENLYRI
                     AIQYYGSGSPENVEKIRRANGLSGNNIRNGQQIVIP"
     gene            1628..1744
                     /locus_tag="POB55_002217"
     CDS             1628..1744
                     /locus_tag="POB55_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501163.1"
                     /translation="MLFNKVLSRILAYQLNFLHSHQKDKTPQSTNLTRKNKK"
     gene            1905..2891
                     /locus_tag="POB55_002218"
     CDS             1905..2891
                     /locus_tag="POB55_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpdA family putative bacillithiol disulfide
                     reductase"
                     /protein_id="HDI6501164.1"
                     /translation="MQKVESIIIGGGPCGLSAAIEQKRKGIDTLIIEKGNVVESIYNY
                     PTHQTFFSSSDKLSIGDVPFIVEESKPRRNQALVYYREVVKHHQLKVNAFEEVLTVKK
                     MNNKFTITTTKDVYECRFLTIATGYYGQHNTLEVEGADLPKVFHYFKEAHPYFDQDVV
                     IIGGKNSAIDAALELEKAGANVTVLYRGGDYSPSIKPWILPNFTALVNHEKIDMEFNA
                     NVTQITEDTVTYEVNGESKTIHNDYVFAMIGYHPDYEFLKSVGIQINTNEFGTAPMYN
                     KETYETNIENCYIAGVIAAGNDANTIFIENGKFHGGIIAQSMLAKKQTPLES"
     gene            complement(3006..3974)
                     /locus_tag="POB55_002219"
     CDS             complement(3006..3974)
                     /locus_tag="POB55_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HDI6501165.1"
                     /translation="MKHLLVIHTGGTISMSQDQSNKVVTNDTNPISMHQDVINQYAQI
                     DELNPFNVPSPHMTIQHVKQLKDIILEAVTNKYYDGFVITHGTDTLEETAFLLDLILG
                     IEQPVVITGAMRSSNEIGSDGLYNYISAIRVASDEKARHKGVMVVFNDEIHTARNVTK
                     THTSNTNTFQSPNHGPLGVLTKDRVQFHHMPYRQQALENVNDKLNVPLVKAYMGMPGD
                     IFSFYSREGIDGMVIEALGQGNIPPSALEGIQQLVSLNIPIVLVSRSFNGIVSPTYAY
                     DGGGYQLAQQGFIFSNGLNGPKARLKLLVALSNNLDKAEIKSYFEL"
     gene            4051..4710
                     /locus_tag="POB55_002220"
     CDS             4051..4710
                     /locus_tag="POB55_002220"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HDI6501166.1"
                     /translation="MKAINIALDGPAAAGKSTIAKRVASELSMIYVDTGAMYRALTYK
                     YLKLNKTEDFAKLVDQTTLDLTYKADKGQCVILDNEDVTDFLRNNDVTQHVSYVASKE
                     PVRSFAVKKQKELAAEKGIVMDGRDIGTVVLPDADLKVYMIASVEERAERRYKDNQLR
                     GIESNFEDLKRDIEARDQYDMNREISPLRKADDAVTLDTTGKSIEEVTDEILAMVSQI
                     K"
BASE COUNT         1899 a          771 c          893 g         1405 t
ORIGIN      
        1 tttatgtaaa atcattgtaa tagaatagaa aggaagatgg ctatgtctaa taattttaaa
       61 gatgactttg aaaaaaatcg tcaatcgata gacacaaatt cacatcaaga ccatacggaa
      121 gatgttgaaa aagaccaatc agaattagaa catcaggata caatagagaa tacggagcaa
      181 cagtttccgc caagaaatgc ccaaagaaga aaaagacgcc gtgatttagc aacgaatcat
      241 aataaacaag ttcacaatga atcacaaaca tctgaagaca atgttcaaaa tgaggctggc
      301 acaatagatg atcgtcaagt cgaatcatca cacagtactg aaagtcaaga acctagccat
      361 caagacagta cacctcaaca tgaagaggaa tattataata agaatgcttt tgcaatggat
      421 aaatcacatc cagaaccaat cgaagacaat gataaacacg agactattaa agaagcagaa
      481 aataacactg agcattcaac agtttctgat aagagtgaag ctgaacaatc tcagcaacct
      541 aaaccatatt ttgcaacagg tgctaaccaa gcaaatacat caaaagataa acatgatgat
      601 gtaactgtta agcaagacaa agatgaatct aaagatcatc atagtggtaa aaaaggcgca
      661 gcaattggtg ctggaacagc gggtgttgca ggtgcagctg gtgcaatggg tgtttctaaa
      721 gctaagaaac attcaaatga cgctcaaaac aaaagtaatt ctgacaagtc gaataactcg
      781 actgaggata aagtgtctca agataagtct aaagatcatc ataatggcaa aaaaggtgca
      841 gcgatcggtg ctggaacagc aggtttggct ggaggcgcag caagtaaaag tgcttctgcc
      901 gcttcaaaac cacatgcctc taataatgca agccaaaacc atgatgaaca tgacaatcat
      961 gacagagata aagaacgtaa aaaaggtggc atggccaaag tattgttacc attaattgca
     1021 gctgtactaa ttatcggtgc attagcgata tttggaggca tggcattaaa caatcataat
     1081 aatggtacaa aagaaaataa aatcgcgaat acaaataaaa ataatgctga tgaaagtaaa
     1141 gacaaagaca catctaaaga cgcttctaaa gataaatcaa aatctacaga cagtgataaa
     1201 tcaaaagagg atcaagacaa agcgactaaa gatgaatctg ataatgatca aaacaacgct
     1261 aatcaagcga acaatcaagc acaaaataat caaaatcaac aacaagctaa tcaaaatcaa
     1321 caacagcaac aacaacgtca aggtggtggc caaagacata cagtgaatgg tcaagaaaac
     1381 ttataccgta tcgcaattca atactacggt tcaggttcac cggaaaatgt tgaaaaaatt
     1441 agacgtgcca atggtttaag tggtaacaat attagaaacg gtcaacaaat cgttattcca
     1501 taatataact atataaattg taactgaact gctatataca aacgtgcata ttaaaagtat
     1561 ctctaaatat tttggagaac ttaaattgcg cttataagta tgtagcggtt ttttcatttt
     1621 tcaaagtttg ttatttaaca aggtgttgtc tcgaatattg gcatatcaat taaatttttt
     1681 acatagtcat caaaaagata aaacaccaca atcaacaaat ttaacgagga agaataaaaa
     1741 ataaatcaac atattaaatt gtagtgttat tcaactccgt agctaacaat tctctatgca
     1801 tattaaacaa attgtcgaaa atatatcata aatcttcaag cacagactta gcgcatcaat
     1861 cactgaactg ttataatagt ttgggattaa aggaggccga aacaatgcaa aaagttgaaa
     1921 gtatcataat tggtggaggg ccatgcggat taagtgcggc tattgaacaa aaaagaaaag
     1981 gtattgatac cttaattatt gaaaagggta atgtcgttga atcaatatat aattatccta
     2041 ctcaccaaac atttttctca tcaagtgata aattaagtat tggggacgta ccgtttatcg
     2101 ttgaagaaag taaaccaaga cgtaatcaag cgctagttta ttaccgagaa gttgtaaaac
     2161 atcatcaatt aaaagtaaat gcatttgaag aagtattaac tgttaaaaaa atgaataata
     2221 aatttacaat tactacgacg aaagatgttt atgaatgtcg atttttaaca atcgcgacag
     2281 gctattatgg tcagcataat acattagagg ttgaaggtgc ggatttacct aaagtgttcc
     2341 attattttaa agaggcacat ccgtattttg atcaagatgt tgttattatt ggtggtaaga
     2401 attcggctat cgatgctgct ttggagttgg aaaaagctgg tgctaacgtg acggttctat
     2461 atcgtggtgg agattattcg ccttcaatta aaccgtggat acttccaaat ttcacagcat
     2521 tagtaaatca tgaaaaaatt gacatggaat ttaatgctaa tgttacccaa ataactgaag
     2581 atactgtgac ttatgaagta aatggtgaaa gtaaaacgat acacaatgat tatgtttttg
     2641 cgatgattgg ttatcatccc gattatgaat ttttaaaatc tgtaggtatt caaattaata
     2701 caaatgaatt tggaacagcg cctatgtata ataaagagac atacgaaaca aatatcgaaa
     2761 attgctacat tgcaggtgtg attgctgcag ggaatgatgc gaataccatt tttattgaaa
     2821 atggtaaatt ccacggtggc attattgctc aaagcatgct agctaagaaa caaacgccgt
     2881 tagaatcata aaaataaagg tctatgtact aaatatctta gttatacaac gactgacatt
     2941 catgatatgt caaatgaggt tgatgactaa tgattgtaca tagacctttt tatgttacgt
     3001 aatcattata attcaaaata tgatttgatt tcagctttat ctaaattgtt gcttaacgca
     3061 actaataatt ttaatcttgc ttttggacca ttcaaaccgt tagaaaaaat aaaaccttgt
     3121 tgtgcgagtt ggtaaccacc accatcgtat gcgtaagttg gactcacaat accattaaag
     3181 gaacgtgaaa ctagcacaat aggtatattt aaagatacta attgttgaat gccttctaat
     3241 gcgcttggag gtatgttgcc ttgtcctaac gcttcaataa ccataccatc gataccttct
     3301 cgactataaa aactaaaaat gtcacctggc atacccatat atgcttttac taatggtaca
     3361 tttagtttgt cattgacatt ttccaatgct tgttggcgat atggcatatg atggaattgc
     3421 acacgatcct ttgtcaatac acctagcgga ccatgatttg gactttgaaa tgtgtttgta
     3481 ttagacgtat gtgttttggt aacattacgc gccgtatgaa tttcatcatt aaatacaacc
     3541 atcacgcctt tatgacgggc cttttcatca gaggcaacgc gaatagcgga aatataatta
     3601 tataatccgt cagaaccaat ttcattagac gagcgcattg cgccagtaat aacaacaggt
     3661 tgctcgatac ctaatattaa atcaagtaaa aaggcagttt cttctaacgt atcggtacca
     3721 tgcgtgataa cgaaaccatc ataatattta tttgttactg cttctaaaat aatatccttt
     3781 aattgtttaa catgttggat tgtcatatga ggtgatggta cattaaaagg atttaattca
     3841 tctatttgtg catattgatt tatgacatct tgatgcattg aaatagggtt ggtatcattt
     3901 gttactactt tattagattg gtcttgtgac atactaatgg tgccaccagt atgaataaca
     3961 agtagatgtt tcatatattt cctcctatat ttaatttacc taattatgat aaaatattat
     4021 tcataaaacg acaaggaagg gaaatgacgc atgaaagcca ttaatattgc attagatggt
     4081 ccagctgctg ccggaaaaag tacaattgcg aaacgtgtag ccagcgaact atcaatgatt
     4141 tatgtcgata caggagcaat gtatcgtgca ttaacataca aatatttaaa attaaacaaa
     4201 actgaggact ttgcaaaact agttgaccaa acaacattag atttaactta taaagcagat
     4261 aaaggtcaat gtgtcatttt agataacgaa gatgtaacag actttttaag aaataatgat
     4321 gtgacgcaac atgtttcata cgttgcatct aaagagccag tacgttcatt cgccgttaaa
     4381 aaacaaaaag agttagctgc agaaaaaggt atcgtaatgg atggtcgcga tatcggaact
     4441 gtagtgctac cagatgcaga tttaaaagta tatatgattg catcagttga agagcgagca
     4501 gaaagaagat ataaagataa tcaattaaga ggtatcgaat caaattttga agatttaaaa
     4561 cgtgatattg aagctcgtga tcaatatgac atgaaccgtg aaatatcacc attaagaaaa
     4621 gcagatgatg cagtgacatt agatacgaca ggcaagtcga ttgaagaagt tactgacgaa
     4681 attttagcga tggtgagtca aattaaataa ttaagaagct aacattaata aaatttataa
     4741 ttacttgatg aacggaaaag gctgttttta ttctaatcga atagaaatgg tcttttgtta
     4801 atttataaac gaataacgat aatactagaa ataaaagtgc gtaattaaac caagcatatt
     4861 agtaattcaa ctgatttata aacaacctat gaattgtatt aacaaaaaag ctaaaatagc
     4921 atgttattta aatttacttt tttaaaatta aagagatgct ttaaaata
//
LOCUS       DAMZKC010000162         4914 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.164, whole genome shotgun sequence.
ACCESSION   DAMZKC010000162 DAMZKC010000000
VERSION     DAMZKC010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4914
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.164"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            41..367
                     /locus_tag="POB55_002221"
     CDS             41..367
                     /locus_tag="POB55_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019468012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor antagonist"
                     /protein_id="HDI6501167.1"
                     /translation="MNLNIETTTQDKFYEVKVGGELDVYTVPELEEVLTPMRQDGTRD
                     IYVNLENVSYMDSTGLGLFVGTLKALNQNDKELYILGVSDRIGRLFEITGLKDLMHVN
                     EGTEVE"
     gene            369..848
                     /gene="rsbW"
                     /locus_tag="POB55_002222"
     CDS             369..848
                     /gene="rsbW"
                     /locus_tag="POB55_002222"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001190828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma B factor RsbW"
                     /protein_id="HDI6501168.1"
                     /translation="MQSKEDFIEMRVPASAEYVSLIRLTLSGVFSRAGATYDDIEDAK
                     IAVSEAVTNAVKHAYKENNNVGIINIYFEILEDKIKIVISDKGDSFDYETTKSKIGPY
                     DKNENIDFLREGGLGLFLIESLMDEVTVYKESGVTISMTKYIKKEQVRNNGERVEIS"
     gene            823..1593
                     /gene="sigB"
                     /locus_tag="POB55_002223"
     CDS             823..1593
                     /gene="sigB"
                     /locus_tag="POB55_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigB"
                     /protein_id="HDI6501169.1"
                     /translation="MAKESKSANEVSPEQINQWIKEHQENKNTDAQDKLVKHYQKLIE
                     SLAYKYSKGQSHHEDLVQVGMVGLIGAINRFDMSFERKFEAFLVPTVIGEIKRYLRDK
                     TWSVHVPRRIKEIGPRIKKVSDELTAELERSPSISEIADRLEVSEEEVLEAMEMGQSY
                     NALSVDHSIEADKDGSTVTLLDIMGQQDDHYDLTEKRMILEKILPILSDREREIIQCT
                     FIEGLSQKETGERIGLSQMHVSRLQRTAIKKLQEAAHQ"
     gene            2026..4176
                     /locus_tag="POB55_002224"
     CDS             2026..4176
                     /locus_tag="POB55_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HDI6501170.1"
                     /translation="MDNQLINSIIEKYQFSKKQIEAVLTLLEEKNTVPFIARYRKEQT
                     GGLDEVQIKQIDDEYQYMVNLQKRKEEVIKNIEQQGLLTEELKNDILKQNKLQRVEDL
                     YRPFKQKKKTRATEAKRKGLEPLAIWMKARKHEVSIEEKAQQFINEEVQSVEDAIKGA
                     QDIIAEQISDNPKYRTKILKDMYHQGVLTTSKKKNAEDEKGIFEMYYAYSEPIKRIAN
                     HRVLAVNRGEKEKVLSVKFEFDTTSVEDFIARQEINHNNVNRSYILEAIKDSLKRLIV
                     PSIEREIHADLTEKAENHAIDVFSENLRNLLLQPPMKGKQILGVDPAFRTGCKLAVIN
                     PFGTFIAKGVIYPHPPVSKKEAAEKDFVQMVKAYDVQLIAIGNGTASRETEQFVADLI
                     KKHQLPVQFIIVNEAGASVYSASEIARDEFPDFQVEERSAVSIGRRVQDPLSELVKID
                     PKSIGVGQYQHDVNQKALENALTFVVETAVNQVGVDVNTASSSLLQYVSGLSSQIAKN
                     IIAYREENGAIKHNKELSKIKRLGAKTFEQSIGFLRIVDGSEPLDNTSIHPESYKVTY
                     QLLDKLGFGGNDLGSDALKAKLNSLDMDELAIELQVGVPTLEDIIKSLKAPNRDPRDE
                     FETPILKSDVLSIEDLQEGMKLSGTVRNVVDFGAFVDIGVKQDGLVHVSKLSKKFVKN
                     PMDIVSVGDIVDVWIYSIDKNKDKVSLTMIDPHE"
     gene            4169..4624
                     /locus_tag="POB55_002225"
     CDS             4169..4624
                     /locus_tag="POB55_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HDI6501171.1"
                     /translation="MNNDKLQRMVENLSEEKFGRTFRHCAYFNKRLRTTGGRYLLKSH
                     DIEINPKQYEHYGEDAVVKIILHELCHYHLHIAGKGYQHKDQDFKRLSQQVGAPRFCN
                     SIESYQQRANYEYYCTKCHAKYIRIRKVDTNRMRCGHCNGKLRMKRQLK"
BASE COUNT         1877 a          610 c          960 g         1467 t
ORIGIN      
        1 aatgaagata aatatttata ataagtagga gtgtaatgaa atgaatctta atatagaaac
       61 aaccactcaa gataaatttt acgaagttaa agtcggtgga gaattagatg tttatactgt
      121 gcctgaatta gaagaggttt taacacctat gagacaagat ggaactcgtg atatttatgt
      181 taatttagaa aatgtgagtt atatggattc gacaggttta ggtttattcg taggtacatt
      241 aaaagcatta aaccaaaatg ataaagaact atacatttta ggtgtgtcag atcgtatcgg
      301 tagactattt gaaattactg gtcttaagga tttaatgcat gttaatgaag gaacggaggt
      361 cgaataacat gcaatctaaa gaagatttta tcgaaatgcg cgtgccagca tcggcagagt
      421 atgtaagttt aattcgttta acactttctg gcgttttttc gagagctggt gctacatatg
      481 atgatattga agatgccaag attgcagtta gtgaagctgt gacaaatgca gttaaacatg
      541 catacaaaga aaataacaat gtaggcatta ttaacatata ttttgaaatt ttagaagata
      601 aaattaaaat tgttatttct gataaaggtg acagttttga ttatgaaaca actaaatcaa
      661 aaataggtcc ttacgataaa aacgaaaata tagacttttt acgcgaaggt ggcctaggtt
      721 tatttttaat cgaatcttta atggatgaag tgacagtata taaagaatct ggtgtgacaa
      781 tcagtatgac taagtatata aaaaaagagc aggtgcgaaa taatggcgaa agagtcgaaa
      841 tcagctaatg aagtttcacc tgagcaaatt aaccaatgga ttaaagaaca ccaagaaaat
      901 aagaatacag atgcacagga taagttagtt aaacattacc aaaaactaat tgagtcattg
      961 gcatataaat attctaaagg acaatcacat cacgaagatt tagttcaagt tggtatggtt
     1021 ggtttaatag gtgccataaa tagattcgat atgtcctttg aacggaagtt tgaagccttt
     1081 ttagtaccta ctgtaatcgg tgaaatcaaa agatatctac gagataaaac ttggagtgta
     1141 catgttccga gacgtattaa agaaattggg ccaagaatca aaaaagtgag cgatgaacta
     1201 accgctgaat tagagcgttc accttctatc agtgaaatag ctgatcgatt agaagtctca
     1261 gaagaagaag tgttagaagc aatggaaatg ggacaaagtt ataatgcgtt aagtgttgat
     1321 cattccattg aagctgataa agatggttca actgttacgc tattagatat tatggggcaa
     1381 caagatgacc attatgactt aactgaaaaa cgtatgattt tagaaaaaat attacctata
     1441 ttatctgatc gcgaacgaga aatcatacaa tgtacgttta ttgaaggatt gagtcaaaaa
     1501 gagacaggtg agcgtatcgg tttaagtcaa atgcatgtat cacgacttca gagaacggca
     1561 attaagaaat tacaagaagc agcacatcaa tagaatttgt ttattaatga tacgttttat
     1621 aatgaaaaat ccatataatt atcccttgat tattaaattg aaatcgaggg gtatttttaa
     1681 tttaattaag attttcgaat taatacatta ttagtgtagt ttaatgtgta tccacataaa
     1741 tgtcgcgata tagtattaat aatttaagtg aagaagatat ctaattgtcg ttttaaatag
     1801 gtgggttgct attagaataa aaaagtagtc ttagattatg aaatttagaa atgatggtgt
     1861 gtcattttca ataatcttag tgcgttttaa aatatagtat gacctaatca ttcgttttaa
     1921 atgttttgga agtgaaaatt acattaagta tcatacctta atagaagtat tttagaatat
     1981 gttaaaataa atgagtaaat ttaagaaaaa gtgtgggtta agtaaatgga caatcaattg
     2041 attaattcaa tcatagagaa atatcaattt agtaaaaaac aaattgaagc agtattaaca
     2101 ctgctagaag aaaaaaatac agtaccattt attgcgaggt atcgaaaaga gcaaactggt
     2161 ggactagatg aagttcaaat aaagcaaatt gatgacgaat accaatatat ggtcaattta
     2221 caaaaacgta aagaagaagt tatcaaaaat atagaacagc aaggattact tactgaggaa
     2281 ttaaagaacg atattttaaa acagaacaaa ttacaacgtg ttgaagacct atataggcct
     2341 tttaaacaaa agaaaaagac aagggcaact gaggcgaaac gtaaagggtt agagccatta
     2401 gcgatatgga tgaaggcacg taaacatgaa gtctcaattg aagaaaaagc acaacaattt
     2461 ataaatgaag aagtgcaatc ggttgaagat gctatcaaag gtgcacaaga tattattgcg
     2521 gaacaaattt cagataatcc taaatataga acaaaaattt taaaagatat gtatcatcaa
     2581 ggtgtgttaa ctacatctaa aaagaaaaat gctgaagatg aaaaaggtat ttttgaaatg
     2641 tactatgcat atagtgagcc aattaaacgc attgctaatc atagagtttt agctgttaat
     2701 cgtggtgaaa aagagaaagt attatctgta aagtttgaat tcgatacgac atcagtagag
     2761 gatttcattg cacgtcaaga aatcaatcat aataatgtaa atcgcagtta tattttagag
     2821 gcgattaaag atagcttgaa acgcttaatt gtcccttcga tagagcgtga aatccatgct
     2881 gatttaactg aaaaagctga aaatcatgca atagatgttt ttagtgaaaa cttaagaaat
     2941 ctattactgc aacctccaat gaaaggtaaa caaatattag gcgtagatcc agcatttaga
     3001 acaggttgta aattagcagt cattaaccca ttcggtactt ttatagcaaa aggtgtgatt
     3061 tatccgcatc caccagtttc taaaaaagag gcagcagaga aggattttgt acaaatggtt
     3121 aaagcgtatg atgtgcaatt aattgcaatt ggcaatggta ctgcaagtcg tgaaacagaa
     3181 caatttgttg cagatttaat taaaaagcat cagttgccag tacaattcat cattgtcaat
     3241 gaagcgggcg cttcagtata ctcagcatca gaaattgcta gagatgaatt tcctgatttt
     3301 caagtggaag aaagaagtgc cgtatcaatt ggacgacgcg ttcaagatcc attaagtgaa
     3361 ttagttaaaa tcgatcctaa atctattggt gtcggacaat accagcatga tgttaatcaa
     3421 aaagcactag aaaatgcatt aactttcgtt gttgaaacag cagttaacca agtaggtgtt
     3481 gatgtcaata cagcatcatc atcattatta caatatgtgt ctggtctaag ttcgcaaatt
     3541 gctaaaaata taattgcata tagagaagaa aatggcgcaa taaagcataa taaagagtta
     3601 agcaagatta agcgtttggg tgctaaaact tttgagcaga gtataggatt tttaagaata
     3661 gtagacggtt cagagccatt ggataataca tcgattcatc ccgaaagcta taaagtaacg
     3721 tatcagttat tagataaact cggatttggt ggcaatgatt taggtagtga tgcattaaaa
     3781 gctaaattaa attcattgga tatggatgaa ctggcgattg aattacaagt cggtgtacct
     3841 acattagagg atattattaa atccttgaaa gcacctaatc gagatccaag ggacgagttt
     3901 gaaacaccaa tccttaaatc cgatgtgcta tcaattgaag atttacaaga aggaatgaaa
     3961 ttgagtggta cagttagaaa tgtagtagat tttggagcat ttgtagatat aggtgttaaa
     4021 caagatggac ttgtccatgt ttcgaaactt tctaagaagt tcgtaaaaaa tccaatggat
     4081 attgtcagtg ttggtgatat cgttgacgtt tggatttaca gtattgacaa aaataaagac
     4141 aaagtatcat taacgatgat tgatccacat gaataatgac aaacttcagc gaatggttga
     4201 gaatctttcg gaagaaaaat ttggacgtac gtttcgacat tgtgcatatt ttaataaaag
     4261 gttacgtaca acaggcggac gttatctttt aaagtcccat gatattgaaa ttaatccaaa
     4321 acaatatgag cattatggag aagatgctgt tgtaaaaatt atactgcacg aattatgtca
     4381 ttatcacttg catattgctg gtaaaggtta tcaacataaa gatcaagatt ttaaacgatt
     4441 gagccaacag gttggagcgc ctagattttg taacagcatt gaaagctatc aacaacgggc
     4501 aaattatgaa tattattgca ctaaatgtca tgcaaaatat atacgtatcc gtaaagttga
     4561 tacgaatcgt atgcgttgcg gacattgcaa tggaaaatta agaatgaaaa gacaattgaa
     4621 ataagtacaa tacaaataga gctaattcgt gtatatgtaa gattatttat gagttgtatg
     4681 gaatttatat agaatttatc agtgagttat tttgattggt aaacatagta attgaataag
     4741 aagaaattgt gtttataaaa tgtttaaagc cgagattgtc gtaatttaat acttcgcaat
     4801 ctcggctttt ctacttatga tataggtaat ggcataaatt gtgacttgat aggatgaaat
     4861 taaattgatt agattaaaaa tcttgacgta ttttagtaat aaataatata taaa
//
LOCUS       DAMZKC010000163         4864 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.165, whole genome shotgun sequence.
ACCESSION   DAMZKC010000163 DAMZKC010000000
VERSION     DAMZKC010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4864
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.165"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            47..526
                     /locus_tag="POB55_002226"
     CDS             47..526
                     /locus_tag="POB55_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501172.1"
                     /translation="MSDYTKLSKSPKSALLYYYITNGLEFILSVAVYVIFYFIWLRFE
                     WPHYLLYILLVLGALSTLKLCIKPLWQYHCRFYKVDQLSVQYRTSFLIYKEETSRIER
                     LQYLSIKSNPISKVLNLYKVGFMTAGHTIYLPMMSHDDVKIIEARTMSNLRGVESDV"
     gene            519..2102
                     /locus_tag="POB55_002227"
     CDS             519..2102
                     /locus_tag="POB55_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HDI6501173.1"
                     /translation="MSEPQKLHPISYFNGIINAIKQNIVVFFIFIIFQLKDFDYTNPE
                     SYLWIGIVFVFFLISYISQIVGIMNTRYWIEDNYFILTTGIFNKKRKELNIKRIQSVD
                     MTQGVVNQIIGGVDLQIKTPSDGIVLSVISKKQGEYLERYIDQLQTELKTEVAQKSSA
                     EKNDNCNHDIEGNVEDIAEGRLDEVQNTDSMLNQKNKRIPIYQMNFKESLFMAMTSGA
                     IGVTLAALVPIYGAVRELIPWSKLNHEISQWVQIVSISVLIIILLVLIVAYIIGTIIT
                     MIRFYGFSVMLENEQLKIEYGLFNKKKVTVPTKRLQAVVERQSYIRRLFGYTAIHFII
                     TSDFENKKIEDDDNDSGSVPILPFIKRDKAYQIIQNLVPDLSYQQVNEGMPLSGFHRH
                     FLIPSIIVIAMAGIGCYFWNQLLLLFTIITIVIIGLFVIKGYLYVKHAGFKMTEEQIT
                     IQNFELLKRNIYYFKAHHIIGFDTWQHTLLKRNNLNNFSFIVAKGVGAQSINLKYARS
                     EDANKLKSWYLRGENDEGL"
     gene            2089..2580
                     /locus_tag="POB55_002228"
     CDS             2089..2580
                     /locus_tag="POB55_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HDI6501174.1"
                     /translation="MRDYNFMSPHAKKVMRLSAVLFWIPIALVLLIAFNILNWLFWEI
                     LDNNISIVSSVIIILLVAVFTLVIAPVYRYKHCRYAFEKHHLRVRNGILFLDEKVIPY
                     FRIQNIDIDVGPIMRRYRLATLTLYTAGGQAEIELIDRSEARRLKRWLNNERHLENNK
                     NEE"
     gene            2584..2943
                     /locus_tag="POB55_002229"
     CDS             2584..2943
                     /locus_tag="POB55_002229"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HDI6501175.1"
                     /translation="MIHGIGVDLIEIDRIKVLYSKQPKLVERILTKNEQHKFNNFTHE
                     QRKIEFLAGRFATKEAFSKALGTGLGKHVAFNDIDCYNDELGKPKIDYEGFIVHVSIS
                     HTEHYAMSQVVLEKSAF"
     gene            3009..4157
                     /gene="alr"
                     /locus_tag="POB55_002230"
     CDS             3009..4157
                     /gene="alr"
                     /locus_tag="POB55_002230"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDI6501176.1"
                     /translation="MSDKYYRSAYMNVDLNAVASNFKVFSTLHPNKTVMAVVKANAYG
                     LGSVKVARHLMENGATFFAVATLDEAIELRMHGITAKILVLGVLPAKDIDKAIQHRVA
                     LTVPSKQWLKEAIKNISGEQEKKLWLHIKLDTGMGRLGIKDTNTYQEVIEIIQQYEQL
                     VFEGVFTHFACADEPGDMTTEQYQRFKDMVNEAIKPEYIHCQNSAGSLLMDCQFCNAI
                     RPGISLYGYYPSEYVQQKVKVHLKPSVQLIANVVQTKTLQAGESVSYGATYTATDPTT
                     IALLPIGYADGYLRIMQGSFVNVNGHQCEVIGRVCMDQTIVKVPDQVKAGDSVILIDN
                     HRESPQSVEVVAEKQHTINYEVLCNLSRRLPRIYHDGDQRFVTNELLK"
     gene            4242..4412
                     /gene="mazE"
                     /locus_tag="POB55_002231"
     CDS             4242..4412
                     /gene="mazE"
                     /locus_tag="POB55_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HDI6501177.1"
                     /translation="MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDC
                     NETYLSSNSTNE"
     gene            4409..4771
                     /locus_tag="POB55_002232"
     CDS             4409..4771
                     /locus_tag="POB55_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDI6501178.1"
                     /translation="MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVA
                     AITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIRTLDKKRLKEKLTYLSDDKMKEV
                     DNALMISLGLNAVAHQKN"
BASE COUNT         1715 a          664 c          934 g         1551 t
ORIGIN      
        1 aatagattat aaatccgtat aattttttat ttggggagag gggtatatgt cagattatac
       61 taagttgtct aagagtccta agtcagcatt gttgtattat tacattacga atggcttaga
      121 atttatattg agtgtagctg tgtatgtcat tttttatttt atatggttaa gatttgaatg
      181 gccacattac ttactttata ttttgttggt tttaggtgct ctgagtacat taaaattatg
      241 tattaagcct ttatggcaat atcattgtcg cttttataaa gttgatcaac taagtgtaca
      301 atatcgtaca tcatttttaa tatataaaga agaaacgagt cgaattgaac gtttacaata
      361 cttgtcgatt aaatctaatc caattagtaa agtgcttaat ttgtataaag ttggatttat
      421 gactgcaggt catactattt atttaccgat gatgtctcat gacgatgtta aaataatcga
      481 agcgagaact atgtcgaatt tgagaggggt tgaaagtgat gtctgaacct caaaaattac
      541 atccgatttc atattttaat ggcataatca atgctatcaa acaaaacata gtcgtctttt
      601 ttattttcat tatctttcaa ttgaaagatt ttgattatac gaatcccgaa tcatatttat
      661 ggataggtat tgtatttgta ttctttttaa tatcatacat ttctcaaatt gtcggaatta
      721 tgaacacgcg atattggatt gaggataatt atttcatttt aacaacaggt atttttaata
      781 aaaagcgtaa agaattaaat ataaaacgta ttcagtccgt tgatatgaca caaggggtcg
      841 tcaatcaaat tattggtggt gtggatttac aaatcaaaac accaagtgat ggtattgtct
      901 taagtgttat ttcgaaaaaa caaggtgaat atcttgaacg ctatattgat caattgcaaa
      961 cagagttgaa gacagaagtg gcgcaaaagt ctagtgctga gaagaatgac aactgtaatc
     1021 atgacattga aggtaatgtg gaagacatcg cagaaggtcg cttagatgaa gtgcagaaca
     1081 ctgatagtat gcttaatcag aaaaataaac gcattcctat atatcagatg aattttaaag
     1141 aatcactatt tatggcaatg accagtggcg ccattggtgt aacgttagca gctttagtac
     1201 cgatttacgg ggctgtaaga gagttgatcc catggtcgaa gctaaatcat gaaattagtc
     1261 aatgggttca aattgtaagt atttcagtat taatcatcat tttactagta ttaatagtgg
     1321 catatattat tggaacgatt ataacgatga taagatttta tggcttttcg gtgatgcttg
     1381 aaaatgagca acttaaaatt gaatatggtc tctttaacaa gaagaaagta actgtaccaa
     1441 cgaaacgatt gcaagctgtt gttgaacgtc agtcatatat acgaagatta tttggctaca
     1501 cagcaataca ttttatcatc acaagtgatt ttgaaaataa aaaaatcgaa gatgatgaca
     1561 atgattcagg tagtgtgccg atattgccgt ttattaaacg agacaaagca tatcaaatta
     1621 tccagaattt agtaccagat ttatcttatc aacaagttaa tgaaggtatg cctttgtcag
     1681 gtttccacag acacttttta atacctagta ttattgtcat agctatggca ggtattggct
     1741 gttatttttg gaatcaatta ttattgttat ttacgattat aacaatagtc attattggtt
     1801 tatttgtgat caaaggatat ctttatgtca aacatgcggg ttttaaaatg acagaagaac
     1861 aaattactat ccagaatttc gagctattga aacgaaatat ttattatttt aaagcgcatc
     1921 acattatagg atttgacact tggcaacata cattacttaa acgcaacaat ttaaataatt
     1981 ttagctttat tgttgctaaa ggtgtgggag cccaatcgat taacctaaaa tacgcaagaa
     2041 gtgaagatgc aaataaattg aaatcatggt atttaagagg tgaaaatgat gagggattat
     2101 aattttatgt caccacacgc taaaaaagtg atgaggttaa gtgctgtgct gttttggata
     2161 cccatagcgc ttgtactttt aatcgcattt aatattttga actggctatt ttgggagata
     2221 ttagataaca atatatccat tgttagtagt gtgattataa tattacttgt tgctgttttc
     2281 acgcttgtca tagcgccagt atatcgatat aagcactgca gatatgcatt tgaaaaacac
     2341 catttgcgtg tacgaaacgg cattttattt ttggatgaaa aagtaatacc atattttcgt
     2401 attcaaaata ttgatatcga tgttggtcct ataatgagac gctatcgatt agcaactttg
     2461 accctttata cagcgggtgg tcaagctgaa attgaattaa tagatagaag tgaagcacgt
     2521 agattgaaac gatggttaaa taacgagaga catttagaaa acaataaaaa tgaggaataa
     2581 tcaatgatac atggaattgg tgtagattta atcgaaatcg atcgaataaa agtgttatat
     2641 agtaagcagc caaaattggt tgagcggatt ttaactaaaa atgaacagca caaattcaac
     2701 aatttcacac atgagcaacg taaaattgaa tttttagctg gcaggtttgc tacaaaagaa
     2761 gcgttcagta aagcattagg cacaggctta ggaaaacatg tagcttttaa cgatatagac
     2821 tgttacaacg acgaacttgg caaaccaaag attgattacg aagggttcat cgtacatgtt
     2881 agtatctcac acactgagca ttatgcgatg agccaagttg ttttagaaaa gtcagcattt
     2941 taaatttgtt atacaatgat aattaagatc tagttttaaa acgtgtggga attacaggag
     3001 gaagtaatat gtcggataaa tattatagat ctgcgtatat gaatgtagat ttaaacgctg
     3061 ttgcatcaaa tttcaaagta ttcagtacat tgcatccaaa taaaacagtg atggctgtcg
     3121 ttaaagccaa tgcctatgga ctaggtagtg ttaaagtagc acgtcattta atggaaaatg
     3181 gcgccacatt ttttgctgta gcaacgttag atgaagcgat agaacttaga atgcatggga
     3241 ttactgctaa aattttagtc ttaggtgtgt taccagctaa agatattgat aaagcgatac
     3301 aacaccgagt tgccttaacg gttccgtcta aacagtggtt gaaagaagca attaaaaaca
     3361 tttctggtga gcaagagaaa aagttatggt tgcacattaa attagataca ggaatgggac
     3421 gtttaggtat taaagatact aatacgtatc aagaagtgat tgaaatcatt caacaatatg
     3481 agcaacttgt atttgaaggc gtgtttacac actttgcctg tgctgacgaa ccaggagata
     3541 tgacaactga acaatatcaa cgttttaaag atatggtcaa tgaagcaatt aaacctgaat
     3601 atatacattg tcagaactca gcaggttctc tactaatgga ttgccaattc tgtaatgcaa
     3661 taagaccagg aatttccctt tatggatatt atccatcaga gtatgtacag caaaaagtta
     3721 aagtacacct taaaccaagt gtgcaattaa ttgctaatgt agttcaaaca aagacgctac
     3781 aagcgggtga gtctgtaagt tatggtgcaa cttatacagc tactgaccca actacaatag
     3841 cattgttacc tattggatat gcagatggct atttacgcat aatgcaaggt agctttgtaa
     3901 atgtaaatgg tcatcaatgc gaagttattg gtcgcgtatg tatggatcag acaattgtta
     3961 aagtgccaga tcaagttaaa gctggagatt cggtgatttt aatagataat catagagaaa
     4021 gtccacagtc ggtagaggtg gtagctgaaa agcaacatac tattaattat gaagtgcttt
     4081 gtaacttgtc gagacgtttg ccgcgaatct atcatgatgg tgatcaacgt tttgtaacaa
     4141 atgaattgtt aaaataatat ggtcagtgca tataaaattt gttatcatta gagtaattaa
     4201 aggtcattta ataacttttg gaatcaattg gaggttctca tatgttatct tttagtcaaa
     4261 atagaagtca tagcttagaa caatctttaa aagaaggata ttcacaaatg gctgatttaa
     4321 atctctccct agcgaacgaa gcttttccga tagagtgtga agcatgcgat tgcaacgaaa
     4381 catatttatc ttctaattca acgaatgaat gattagacga ggagatgttt atttagcaga
     4441 tttatcacca gtacagggat ctgaacaagg gggagtcaga cctgtagtca taattcaaaa
     4501 tgatactggt aataaatata gtcctacagt tattgttgcg gcaataactg gtaggattaa
     4561 taaagcgaaa ataccgacac atgtagagat tgaaaagaaa aagtataagt tggataaaga
     4621 ctcagttata ttattagaac aaattcgtac acttgataaa aaacgattga aagaaaaact
     4681 gacgtactta tccgatgata aaatgaaaga agtagataat gcactaatga ttagtttagg
     4741 gctgaatgca gtagctcacc agaaaaatta ggcgtctatt atatgtattt ttcagagata
     4801 aataaaatat tgatataaaa gacaataact ttataataat tataactatt tctaaattct
     4861 gtac
//
LOCUS       DAMZKC010000164         4859 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.166, whole genome shotgun sequence.
ACCESSION   DAMZKC010000164 DAMZKC010000000
VERSION     DAMZKC010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4859
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.166"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            48..1328
                     /locus_tag="POB55_002233"
     CDS             48..1328
                     /locus_tag="POB55_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HDI6501179.1"
                     /translation="MKKLNIALLGLGTVGSGVVKIIEENRQQIQDTLNKDIVIKHILV
                     RDKSKKRPLNISQYHLTEDVNEILNDDSLDIIVEVMGGIEPTVDWLRTALKNKKHVIT
                     ANKDLLAVHLKLLEDLAEENGVALKFEASVAGGIPIVNAINNGLNANNISKFMGILNG
                     TSNFILSKMTKEQTTFEEALDEAKRLGFAEADPTDDVEGVDAARKVVITSYLSFNQVI
                     KLNDVKRRGISGVTLTDINVADQLGYKIKLIGKGIYENGKVNASVEPTLIDKKHQLAA
                     VEDEYNAIYVIGDAVGDTMFYGKGAGSLATGSAVVSDLLNVALFFESDLHTLPPHFEI
                     KTDKTREMMDSDAEINIKEKSNFFVVVNHVKGSIENFENELKAILPFHRSLRVANYDN
                     QSYAAVIVGLESSPEELITKHGYEVDKVYPVEGV"
     gene            1334..2395
                     /locus_tag="POB55_002234"
     CDS             1334..2395
                     /locus_tag="POB55_002234"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HDI6501180.1"
                     /translation="MRRWQGLVEEFKAHLPVNENTPKLTLNEGNTPLIHCENMSKILG
                     IDLYVKYEGANPTGSFKDRGMVMAVTKAKEQGKKIVICASTGNTSASAAAYAARAGLK
                     AIVVIPEGKIALGKLSQAVMYGAEIVSIEGNFDEALEIVKEIAKSGEIELVNSVNPFR
                     IEGQKTGSFEIVQQLDGEAPDILAIPVGNAGNITAYWKGFKEYHEAKGSQLPKMFGFQ
                     AEGASPIVQNKVIKNPETIATAIRIGNPASWDKATNALKESNGLIDSVTDDEILEAYQ
                     LMTTKEGVFSEPASNASIAGLIKLHRQGKLPQGKKVVAILTGNGLKDPDTAISLLDNP
                     IKPLPNDKDSIIDYIKGAL"
     gene            2397..3311
                     /locus_tag="POB55_002235"
     CDS             2397..3311
                     /locus_tag="POB55_002235"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HDI6501181.1"
                     /translation="MSNVLELTIPASTANLGVGFDSIGMALDKFLHLSVKETSGTKWE
                     YIFHDDASKQLPTDETNFIYHVAQQVASKYSVDLPNLCIEMRSDIPLARGLGSSASAL
                     VGAIYIANYFGDIQLSKHEVLQLATEIEGHPDNVAPTIYGGLIAGYYNDVSKETSVAH
                     IDIPDVDVIVTIPTYELKTEASRRALPQKLTHSEAVKSSAISNTMICALAQHNYELAG
                     KLMQQDGFHEPYRQHLIAEFDEVKTIAIQHNAYATVISGAGPTILIFSRKENSGELVR
                     SLNSQVVSCHSELVDINISGVKERIVYQ"
     gene            3369..4172
                     /locus_tag="POB55_002236"
     CDS             3369..4172
                     /locus_tag="POB55_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDI6501182.1"
                     /translation="MTNYKVVVLDMDDTLLNSDNVISEETANYLTAIQDEGYYVVLAS
                     GRPTEGMIPTARDLKLPEHHSYIISYNGSKTINMTNEEVEVSKSIGKQDFDEIVDYCR
                     DRGFFVLTYHDGQIIYDSEHEYMNIEAELTGLPMKRVDDIKAYIQGDVPKVMGVDYVA
                     NITEARIDLNGVFNDNVDATTSKPFFLEFMAKDVSKGNAIKALCHKLGYSVDQVIAFG
                     DSMNDKSMFEVAGLAIAMGNASDELKQYANEVTLDHNENGIPHALKKLL"
     gene            complement(4465..4779)
                     /locus_tag="POB55_002237"
     CDS             complement(4465..4779)
                     /locus_tag="POB55_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501183.1"
                     /translation="MIGQTNLFDDVLKQDERFVIVVQALEEKNGQLLKRTLREYPGLN
                     HKQMNDLFMHLKELFSEESFAENQSAFSITVYTNLDYTADQIYAHVKRFRGKHDWTQT
                     AK"
     gene            complement(4754..4858)
                     /locus_tag="POB55_002238"
     CDS             complement(4754..4858)
                     /locus_tag="POB55_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501184.1"
                     /translation="MLPFFILDILHIVYNIDNNNFSGVKFNDWTNKFI"
BASE COUNT         1751 a          662 c          945 g         1501 t
ORIGIN      
        1 caataataca tttgtttttc tcaaaagcac aaacggaggt acataaaatg aaaaaattaa
       61 atatagcatt attaggatta ggtactgtcg gatctggtgt tgttaaaatc atcgaagaga
      121 accgacagca aattcaagat acattaaata aagatattgt cataaagcat attcttgttc
      181 gagataaatc taaaaagaga ccgctaaata ttagccaata tcatttaact gaagatgtta
      241 atgaaatttt aaatgatgat tcattagata ttatcgttga agtcatggga ggaattgaac
      301 caactgtaga ttggttaaga acagcactta aaaataaaaa acatgttatt accgcaaata
      361 aagatttatt agcagtacat cttaaacttt tagaagattt agcagaagaa aatggtgtag
      421 ctttaaagtt tgaagcgagt gtagcaggtg gtattccgat cgtaaatgcc ataaataatg
      481 gtttgaatgc gaataatatt tcaaaattta tgggaatttt aaatggtacc tctaatttta
      541 ttttatctaa aatgactaaa gagcaaacga catttgagga agcacttgat gaagcgaaaa
      601 gacttggttt tgctgaagcg gatccaactg atgatgtaga aggggtagat gcagcgcgta
      661 aagttgtcat tacatcatat ttatcattta accaagtcat taaattaaac gacgttaaac
      721 gaagaggaat tagtggcgta actttaactg atattaatgt agccgatcaa ctggggtata
      781 aaattaaatt gattggtaag ggaatatatg aaaatggcaa agttaatgca tcggtagaac
      841 caacgttaat tgataaaaag catcaattag cagctgtaga ggatgaatat aacgcgattt
      901 atgtcattgg tgatgccgtt ggtgacacga tgttttatgg aaaaggagca ggcagtttag
      961 caacaggtag tgccgttgtc agtgatttat tgaatgtagc attattcttt gaatcagatt
     1021 tacacacatt gccaccacat tttgaaataa agacagataa aacacgggaa atgatggatt
     1081 cagatgcaga aattaatatt aaagaaaaat ccaatttctt tgtagtagtg aatcatgtca
     1141 aaggttcaat tgaaaatttt gaaaatgagt taaaggcaat attaccattt caccgatcat
     1201 taagagttgc aaattacgat aatcaatcat atgccgctgt tatagttgga ttggaatcat
     1261 caccggaaga attaatcact aagcatggat acgaagttga caaagtatac ccagtagaag
     1321 gagtttaatt ataatgagaa gatggcaagg attagtagaa gagtttaaag cacatttacc
     1381 agtaaatgaa aatacaccaa aattaacatt gaacgaggga aatacaccac tcattcattg
     1441 tgaaaatatg tctaaaatac taggtataga tttatatgtg aagtatgaag gtgccaatcc
     1501 gacaggttca tttaaagatc gcggtatggt aatggctgtg acaaaagcaa aagagcaagg
     1561 taagaaaatt gtaatatgcg cttcgactgg aaatacatca gcgtctgcag cagcatatgc
     1621 agcgagagca ggtttaaaag ctatcgtcgt aataccagaa ggtaaaattg cattaggtaa
     1681 attgtcgcaa gcagtaatgt atggtgcaga aatcgtttct attgaaggaa actttgatga
     1741 agctttagaa attgtaaaag aaattgcaaa aagtggcgaa atagagcttg taaactctgt
     1801 caatccattt agaatcgaag ggcaaaagac aggctcattt gaaattgtac aacaattaga
     1861 cggtgaagca cctgatattt tagcgattcc tgtaggtaat gcaggtaata ttactgcata
     1921 ttggaaaggc tttaaagaat atcatgaagc taaaggttca caattgccga aaatgtttgg
     1981 cttccaagct gaaggcgcat caccaattgt tcaaaataaa gtcattaaaa atcctgaaac
     2041 gattgcaact gctattcgaa ttggtaatcc tgctagttgg gataaggcga ctaatgctct
     2101 taaagaatca aatggattaa tagatagtgt tactgatgat gaaattctag aagcatatca
     2161 gttaatgaca actaaagaag gtgtctttag tgaaccagcg agtaatgctt ctattgcagg
     2221 tttaattaaa ttgcatagac aaggtaaatt acctcaaggt aaaaaagtag ttgctatttt
     2281 aactggtaat ggattaaaag atcctgatac tgctatttca ctactagata atccgataaa
     2341 gccattgcca aatgataaag atagcattat cgattatatt aaaggagctt tataacatgt
     2401 cgaatgtttt ggagttaaca attcctgcat caacagccaa ccttggagtt ggctttgatt
     2461 ctataggtat ggctttagat aaatttttgc atctgtctgt aaaggaaaca tcagggacaa
     2521 aatgggaata tattttccat gatgatgcat ctaagcaatt gcctactgac gaaacaaact
     2581 ttatttatca tgtagcacaa caagttgctt ctaaatatag tgttgacttg cctaatttat
     2641 gtatcgaaat gagaagtgat attccattgg caagagggtt aggttcgtca gcttctgctt
     2701 tagtaggagc tatatatatc gcaaattatt ttggtgatat ccaactgtct aaacatgagg
     2761 tattacaatt agcgactgaa atcgaaggac atcctgataa tgttgcgccg accatttatg
     2821 gtggtttaat cgctggatat tataatgatg tctcgaaaga aacgtcagtt gcacatatcg
     2881 acataccaga cgtggatgtg attgtaacga taccaactta tgaactaaaa acagaagcat
     2941 caagacgtgc tttaccacaa aaattaacac atagtgaagc ggttaaaagt agtgcaatta
     3001 gtaatacaat gatttgtgca ttagcacagc acaattatga attagcaggt aaactcatgc
     3061 aacaagatgg ctttcatgaa ccgtatcgtc agcatttaat tgctgaattt gatgaagtga
     3121 aaacaattgc tattcaacat aatgcctatg caactgtaat tagtggtgct ggaccaacta
     3181 ttttaatatt tagtcgtaaa gaaaatagtg gggaattggt tcgctcttta aatagtcagg
     3241 tagtatcatg ccattctgaa ttggtcgata ttaatatcag tggtgttaaa gaacgaattg
     3301 tataccaata gacgctttat attgtaaaat agtattaaat gcagaataga gaggaggttt
     3361 aatgcgatat gacaaattat aaagttgtcg ttttagatat ggatgacaca ttgctaaatt
     3421 cagataatgt gatatcagaa gaaactgcaa attatttaac agcaattcaa gatgaaggtt
     3481 attatgttgt tttagcatct ggtagaccta ctgaaggtat gattccaact gctagagatt
     3541 taaaattacc tgaacatcat agctatatta ttagttataa cggtagtaaa acgattaaca
     3601 tgactaatga agaagtagaa gtaagtaaat cgattggtaa gcaagatttc gatgaaattg
     3661 tagattattg tcgagataga ggctttttcg ttcttacata tcatgatggt caaattattt
     3721 acgacagcga acatgagtat atgaatattg aagcagaatt aacaggttta ccgatgaaac
     3781 gtgttgatga tatcaaagcg tatattcaag gcgatgtacc caaggtcatg ggtgtagatt
     3841 atgtagcgaa tattacagaa gctagaattg atttgaatgg tgtgttcaat gataatgtag
     3901 atgctacgac aagtaagcca ttcttcttag aatttatggc caaagacgtt tcaaaaggta
     3961 atgcaattaa agcgttatgt cacaaattgg gatattcggt ggatcaagtc attgcttttg
     4021 gtgatagtat gaatgataaa tcaatgtttg aagtcgcagg tctagctatt gctatgggga
     4081 atgcatcaga tgaacttaag caatatgcaa atgaagttac gttggatcat aatgaaaatg
     4141 gtattccaca tgcgctcaaa aaattgttat aaattttaaa ataagcctta acacatgata
     4201 tttgaataag atatcttgtg gttaaggctt tttatttttg tgaaaatgac ttcagttata
     4261 ctatggagga tttgaaatac atattttaga ttagtaatga tatcaaacga atagagtaaa
     4321 tgtatatttt ttgaataaat caagtattaa ctagtcactg aaggtagata aataaatttg
     4381 atcattctga ataataacga atgcattaaa aaatgcagtg acaatctaac attaattgtt
     4441 aaaatcactg caagcatctt gattttattt agctgtttgt gtccagtcat gcttacctct
     4501 gaaacgtttt acatgagcat atatttggtc agcagtataa tctaaatttg tataaactgt
     4561 aatactgaac gctgattggt tttcagcaaa tgattcttcg gaaaataatt cctttaagtg
     4621 cataaataaa tcattcattt gtttatggtt taaaccggga tattccctta aagttctctt
     4681 taataattgt ccgttctttt cttctaatgc ttgaactaca ataacaaatc tttcatcttg
     4741 ctttagtaca tcatcaaata aatttgtttg tccaatcatt gaatttcacc cctgaaaagt
     4801 tattgttatc tatattatac acgatatgca aaatatctaa aataaagaat ggtaacatg
//
LOCUS       DAMZKC010000165         4834 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.167, whole genome shotgun sequence.
ACCESSION   DAMZKC010000165 DAMZKC010000000
VERSION     DAMZKC010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4834
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.167"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            26..496
                     /gene="ssb"
                     /locus_tag="POB55_002239"
     CDS             26..496
                     /gene="ssb"
                     /locus_tag="POB55_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDI6501185.1"
                     /translation="MLNRTILVGRLTRDPELRTTQSGVNVASFTLAVNRTFTNAQGER
                     EADFINIIVFKKQAENVNKYLSKGSLAGVDGRLQTRNYENKEGQRVYVTEVVADSIQF
                     LEPKNSNDTQQDLYQQQVQQTRGQSQYSNNKPVKDNPFANANGPIELNDDDLPF"
     gene            526..1410
                     /locus_tag="POB55_002240"
     CDS             526..1410
                     /locus_tag="POB55_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HDI6501186.1"
                     /translation="MTGWIKLHRKLLDSPIFQNEKLFKVFAYCLMKASHKDHTQLVGR
                     RVVELEKGQFVFGRKRASEELRLKESTVRDYIKLLENLGTIVVKSDNKFSVITVVNWA
                     IYQSMEENSDSKNDNKSTTNQQQMDNKWTTNQQQINTNKNVKNGDNVKNGENEKKKVT
                     AFDFFQDNGFGFITPYNLDDLNYYLDSFENDSDQIVTASLKIAKDRNKVTWGYAKSIL
                     NTWLNANLKSIEQVRAFEKQQLESKKQNYKPFVKQSKEKTPKWLTDSTRETKTPEVDE
                     NLEKDREAFIKRLNSKWE"
     gene            1417..1635
                     /locus_tag="POB55_002241"
     CDS             1417..1635
                     /locus_tag="POB55_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501187.1"
                     /translation="MDAFDKYYLFDHDGNKMFSVTPHFKDGRHLVVGLKHTKFNGRRW
                     YLDDYELKTLIDNEQMELGHQTSLFEYI"
     gene            1644..2048
                     /locus_tag="POB55_002242"
     CDS             1644..2048
                     /locus_tag="POB55_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000401967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HDI6501188.1"
                     /translation="MEIEIKFNETFEAPMGSPRPRFSTKGRYAHTYMPTKYTEHKKYL
                     QNQMPKLNLENALKIELEFYFTPPKSWSKKKKTQAIGQLKVTKPDIDNLMKTVLDACN
                     NYLWKDDNQIAEITSSKRYGIEPKIIIRIEEI"
     gene            2061..2429
                     /locus_tag="POB55_002243"
     CDS             2061..2429
                     /locus_tag="POB55_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501189.1"
                     /translation="MARKARIVTINDKPYRFSKFEMELIESHGITAGMVSKRVKDGWE
                     LNEAMDAPEGTRLSEYREKKTIERLEQARLERKLERKRKREAELRRKKPHLFNVPQKH
                     PRGRYACYLLENDIFVKVKK"
     gene            2433..2675
                     /locus_tag="POB55_002244"
     CDS             2433..2675
                     /locus_tag="POB55_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501190.1"
                     /translation="MTDNARKEYLNQFFGFKRYLYQDNERVAHIHVVNGTYYFHGHIV
                     PGWQSVKKTFDTAEELEIYIKQHGLEYEEQKQLTLF"
     gene            2690..2854
                     /locus_tag="POB55_002245"
     CDS             2690..2854
                     /locus_tag="POB55_002245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501191.1"
                     /translation="MNAEKHMQMMQMLQNCVIDKYVSHDEYEELIAIDKHGNKMFIKF
                     YPNTEDDTNE"
     gene            2847..3098
                     /locus_tag="POB55_002246"
     CDS             2847..3098
                     /locus_tag="POB55_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1024 family protein"
                     /protein_id="HDI6501192.1"
                     /translation="MNNREQIEQSVISASAYNGNDTEGLLKEIEDVYKKAQAFDEILE
                     GMTNAIQHSVKEGVELDEAVGIMAGQVVYKYEEEQENEH"
     gene            3088..3270
                     /locus_tag="POB55_002247"
     CDS             3088..3270
                     /locus_tag="POB55_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501193.1"
                     /translation="MSISVGDKVFNPETNSTLEIVQLVGDIRDTHYKLSDGSIISLID
                     FVVKPIHLIKEEQEND"
     gene            3263..3805
                     /locus_tag="POB55_002248"
     CDS             3263..3805
                     /locus_tag="POB55_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP pyrophosphatase"
                     /protein_id="HDI6501194.1"
                     /translation="MTNTLQVRLLSENARMPERNHKTDAGYDIFSAETVVLEPQEKAV
                     IKTDVAVSIPEGYVGLLTSRSGVSSKTHLVIETGKIDAGYHGNLGINIKNDEERDGIP
                     FLYDDIDAELEDGLISILDIKGNYVQDGRGIRRVYQINKGDKLAQLVIVPIWTPELKQ
                     VEEFESVSERGAKGFGSSGV"
     gene            3842..4048
                     /locus_tag="POB55_002249"
     CDS             3842..4048
                     /locus_tag="POB55_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1381 domain-containing protein"
                     /protein_id="HDI6501195.1"
                     /translation="MTQYLVTTFKDSTGRKHTHITKAKSNQRFTVVEAESKEEAKEKY
                     EKQVKRDAVIKVGQLFENIRECGK"
     gene            4045..4431
                     /locus_tag="POB55_002250"
     CDS             4045..4431
                     /locus_tag="POB55_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501196.1"
                     /translation="MIKKLKNMDGFDIFIVGILSLFGITALLLVVALPIYTVASYQNK
                     EVHQGTITDKYNKRQDKEDKFYIVLDDKQVIENSDLFFKGKFDSADIQARLKVGDKVK
                     VKTIGYRIHFLNLYPVLYEVKKVDKK"
     gene            4428..4577
                     /locus_tag="POB55_002251"
     CDS             4428..4577
                     /locus_tag="POB55_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDI6501197.1"
                     /translation="MIKQILRLLFLLAMYELGKYVTEQVYIMMTANDDVEAPSDYVFR
                     AEVSE"
BASE COUNT         1885 a          683 c         1005 g         1261 t
ORIGIN      
        1 gcccgtttat gttggaggtt tataaatgct aaacagaaca atattagttg gtcgtttaac
       61 tagagaccca gaattaagaa ccactcaaag tggtgtaaat gtagcatcat tcacattagc
      121 agttaaccgc acatttacga atgcacaagg agagcgcgag gcagacttta ttaatatcat
      181 cgtatttaaa aaacaagcag agaacgttaa taaataccta tctaaaggat cgttggcggg
      241 cgtagatggt aggttacaaa cgcggaacta tgaaaataag gaaggtcaac gtgtatacgt
      301 tacggaagtt gttgccgata gtattcaatt tttagaaccg aagaactcaa atgacactca
      361 acaagattta tatcaacaac aagtacaaca aacacgtgga caatcgcaat attcaaataa
      421 caaaccagta aaagataatc cgtttgcgaa tgcaaatggt ccgattgaac taaatgatga
      481 tgatttacca ttctgattta accggtttga aagtgaggtg tgtatatgac tggttggata
      541 aaacttcata gaaaactatt agattcgcct atttttcaga acgaaaagtt attcaaagta
      601 tttgcatatt gtcttatgaa ggctagtcat aaggatcata cacagcttgt tggcagacga
      661 gttgttgaat tagaaaaagg tcaatttgtg ttcgggagaa agcgagcaag cgaagagtta
      721 cgtctcaaag aatccacagt aagagactac ataaagcttt tagaaaacct tggaactatc
      781 gtcgtaaagt ccgacaacaa attttctgtt ataaccgttg tcaattgggc gatttatcaa
      841 agtatggaag aaaattccga cagcaaaaac gacaacaaat caacaacaaa tcaacaacaa
      901 atggacaaca aatggacaac aaatcaacaa caaatcaaca caaacaagaa tgtaaagaat
      961 ggggataatg taaagaatgg tgagaatgag aagaagaagg taaccgcctt cgacttcttc
     1021 caagataacg gattcggttt cataactcct tacaatttag acgatttaaa ttattatctt
     1081 gattcatttg aaaatgattc agatcaaata gttaccgcat cacttaaaat cgctaaagac
     1141 agaaataaag ttacttgggg atatgctaaa agcattttga atacatggct taatgcaaac
     1201 ttgaaatcta ttgaacaagt acgtgcattt gaaaagcaac aacttgaaag caaaaaacaa
     1261 aattataaac ctttcgttaa acaatcaaaa gaaaaaacac ccaaatggct cacagacagc
     1321 acgagagaaa cgaaaacgcc ggaagtagat gaaaaccttg agaaagacag agaagctttt
     1381 attaagcgtc taaatagcaa atgggagtga ttgaaaatgg atgcatttga taaatactat
     1441 ctatttgatc atgacggcaa caaaatgttt tcagttacac cacattttaa agatggacgg
     1501 catttagttg ttggattaaa acacacaaaa ttcaatggtc gacgttggta cttagatgat
     1561 tatgaattaa aaacacttat tgataatgaa caaatggagt taggacacca aacaagctta
     1621 tttgaatata tatgagggat tacatggaga tagaaattaa atttaacgaa actttcgagg
     1681 cacctatggg ctcgcctcga ccgcgtttta gcacaaaagg tagatatgca cacacatata
     1741 tgcctacaaa atatacagaa cataaaaaat atttacaaaa tcaaatgcca aagctaaatc
     1801 tagaaaatgc attaaaaatt gaattagagt tttactttac tccacctaaa tcatggtcga
     1861 agaaaaagaa aactcaagcg attggacaat taaaagtaac taaaccagat atcgataatt
     1921 tgatgaagac agttctagac gcttgtaaca attatttgtg gaaagatgac aatcaaattg
     1981 cagaaataac tagctcaaag cgttatggaa ttgagcccaa aataatcata cgaatagaag
     2041 aaatataaga ggtggaataa atggcgagaa aagcaagaat tgtaacaata aacgataaac
     2101 cttataggtt cagtaaattt gaaatggaat taatagaaag tcacggtata accgctggaa
     2161 tggtttctaa gagagtaaaa gacggttggg aactaaatga agcaatggac gcaccagaag
     2221 gtacgcgttt aagcgagtac agagaaaaga aaacaataga aagactggaa caagctagac
     2281 tcgaacgcaa attggaaaga aagcgaaaga gggaggctga gctaagaaga aagaagccac
     2341 acttgtttaa tgtacctcag aaacatccaa gaggacgtta tgcgtgctac ctgttggaaa
     2401 acgacatatt cgtgaaagtt aagaagtaga tcatgacaga taacgcacgc aaagaatacc
     2461 taaatcaatt ctttggattt aagagatatc tgtatcagga taacgaacga gtggcacata
     2521 ttcatgtagt aaacggcact tattactttc atgggcatat cgtgccaggt tggcaaagcg
     2581 ttaaaaagac atttgatact gctgaagagc tcgaaatata tataaagcaa catggtttgg
     2641 aatacgagga acagaagcaa ctaactttat tttagaggag atggaaatga tgaatgctga
     2701 aaagcatatg caaatgatgc aaatgttaca aaattgtgtg attgataagt atgtatcaca
     2761 cgacgaatac gaagagttaa ttgccataga taagcatggt aataaaatgt ttattaaatt
     2821 ttatccgaat acggaggatg acactaatga ataatcgcga acaaattgaa caatcagtta
     2881 taagtgctag tgcgtataac ggtaatgaca cagagggatt actaaaagag attgaggacg
     2941 tttataagaa agcgcaagcg tttgatgaaa tacttgaggg aatgacaaat gctattcaac
     3001 attcagttaa agaaggtgtt gaacttgatg aagcagtagg gattatggca ggtcaagttg
     3061 tctataaata tgaggaggaa caggaaaatg agcattagtg taggagacaa ggtttttaat
     3121 ccagaaacaa attcaacttt agaaattgta caacttgttg gcgatattag agacacgcat
     3181 tacaagttat ctgacggatc tattattagt cttatagact ttgttgttaa accaattcat
     3241 ttaatcaagg aggagcagga aaatgactaa cacattacaa gtaaggctat tatcagaaaa
     3301 tgctagaatg cccgaacgaa atcataagac ggatgcaggt tatgacatat tctcagctga
     3361 aactgtcgta cttgagccac aagaaaaggc agtgattaaa acagatgtag ctgtaagcat
     3421 accagagggc tatgtcgggc tattaactag ccgtagtggt gtaagtagta aaacacattt
     3481 agtgattgaa acaggcaaga tagacgccgg atatcacggc aatttaggga ttaatattaa
     3541 gaatgatgaa gaacgtgatg gaataccctt tttatatgat gatatagacg ctgaattaga
     3601 agatggatta ataagcattt tagatataaa aggtaactat gtacaagatg gaagaggcat
     3661 aagaagagtt taccaaatca acaaaggcga caaactagca caactggtta tcgtgcctat
     3721 atggacacct gaactaaagc aagtggagga attcgagagt gtttcagaac gtggagcaaa
     3781 aggcttcgga agtagcggag tgtaaagaca tcttagatcg agtcaaggag gttttgggga
     3841 agtgacacaa tacttagtca caacattcaa agattcaaca ggacgtaaac atacacacat
     3901 aactaaagct aagagtaatc aaaggtttac agttgttgag gcagagagta aagaagaagc
     3961 gaaagagaag tacgagaaac aagttaaaag ggatgcagtt attaaagtgg gtcagttgtt
     4021 tgaaaatata agggagtgtg ggaaatgatt aaaaaactta aaaatatgga tgggttcgac
     4081 atctttattg ttggaatact gtcattattc ggtataaccg cattgctact tgttgtcgca
     4141 ttgcctatct atacagtggc tagttaccaa aacaaagaag tacatcaagg gacaattaca
     4201 gataaatata acaaaagaca agataaagag gacaaattct atattgtatt agatgataaa
     4261 caagtcatcg aaaactcaga cttattcttc aaaggaaagt ttgatagcgc agacatacaa
     4321 gctaggttaa aagtaggtga taaagtaaaa gttaagacga ttggatatag aatacacttt
     4381 ttaaatttat atccggtctt atacgaagta aagaaggtag ataaaaaatg attaagcaaa
     4441 tactaagatt attattctta ctagcgatgt atgagttagg taagtatgta actgagcaag
     4501 tatatattat gatgacggct aatgatgatg tagaggcgcc gagtgattac gtctttcgag
     4561 cggaggtgag tgaataatga gaatatttat ttatgatttg atcgttttgc tgtttgcttt
     4621 cttaatatcc atatatatta ttgatgatgg agtgataata aatgcattag gaatttttgg
     4681 tatgtataaa attatagatt ccttttcaga aaatattata aagaggtaga taaaaatgaa
     4741 cgagcaaata ataggaagca tatatacttt agcaggaggt gttgtgcttt attcagttaa
     4801 agagattttt aggtatttta cagattctaa ctta
//
LOCUS       DAMZKC010000166         4787 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.168, whole genome shotgun sequence.
ACCESSION   DAMZKC010000166 DAMZKC010000000
VERSION     DAMZKC010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4787
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.168"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(<1..482)
                     /locus_tag="POB55_002252"
     CDS             complement(<1..482)
                     /locus_tag="POB55_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein"
                     /protein_id="HDI6501198.1"
                     /translation="MKKNIMNKLVLSTALLLLGTTSTQLPKTPISFSSEAKAYNISEN
                     ETNINELIKYYTQPHLSLSNKWLWQKPNGSIHATLQTWVWYSHIQVFGSESWGNINQL
                     RNKYVDIFGTKDEDTVEGYWTYDETFTGGVTPAATSSDKPYRLFLKYSDKQQTIIGGH
                     "
     gene            920..1921
                     /gene="argF"
                     /locus_tag="POB55_002253"
     CDS             920..1921
                     /gene="argF"
                     /locus_tag="POB55_002253"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDI6501199.1"
                     /translation="MKNLRNRSFLTLLDFSRQEVEFLLTLSEDLKRAKYIGTEKPMLK
                     NKNIALLFEKDSTRTRCAFEVAAHDQGANVTYLGPTGSQMGKKETTKDTARVLGGMYD
                     GIEYRGFSQRTVETLAEYSGVPVWNGLTDEDHPTQVLADFLTAKEVLKKDYADINFTY
                     VGDGRNNVANALMQGAAIMGMNFHLVCPKELNPTDELLNRCKNIAAENGGNILITDNI
                     DQGVKGSDVIYTDVWVSMGEPDEVWKERLELLKPYQVNKEMMDKTGNPNVIFEHCLPS
                     FHNADTKIGQQIFEKYGIREMEVTDEVFESKASVVFQEAENRMHTIKAVMVATLGEF"
     gene            1944..2876
                     /gene="arcC"
                     /locus_tag="POB55_002254"
     CDS             1944..2876
                     /gene="arcC"
                     /locus_tag="POB55_002254"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDI6501200.1"
                     /translation="MAKIVVALGGNALGKSPQEQLELVKNTAKSLVGLITKGHEIVIS
                     HGNGPQVGSINLGLNYAAEHNQGPAFPFAECGAMSQAYIGYQLQESLQNELHSIGMDK
                     QVVTLVTQVEVDENDPAFNNPSKPIGLFYNKEEAEQIQKEKGFIFVEDAGRGYRRVVP
                     SPQPISIIELESIKTLIKNDTLVIAAGGGGIPVIREQHDGFKGIDAVIDKDKTSALLG
                     ANIQCDQLIILTAIDYVYINFNTENQQPLKTTNVDELKRYIDENQFAKGSMLPKIEAA
                     ISFIENNPKGSVLITSLNELDAALEGKVGTVIKK"
     gene            3048..4604
                     /locus_tag="POB55_002255"
     CDS             3048..4604
                     /locus_tag="POB55_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HDI6501201.1"
                     /translation="MENTINESEKKKRFKLKMPGAFMILFILTVVAVIATWVIPAGAY
                     SKLSYEPSSQELKIVNPHNQVKKVPGTQQELDKMGVKIKIEQFKSGAINKPVSIPNTY
                     ERLKQHPAGPEQITSSMVEGTIEAVDIMVFILVLGGLIGVVQASGSFESGLLALTKKT
                     KGHEFMLIVFVSILMIIGGTLCGIEEEAVAFYPILVPIFIALGYDSIVSVGAIFLASS
                     VGSTFSTINPFSVVIASNAAGTTFTDGLYWRIGACIVGAIFVISYLYWYCKKIKNDPK
                     ASYSYEDKDAFEQQWSVLKDDDSAHFTLRKKIILTLFVLPFPIMVWGVMTQGWWFPVM
                     ASAFLIFTIIIMFIAGTGKSGLGEKGTVDAFVNGASSLVGVSLIIGLARGINLVLNEG
                     MISDTILHFSSSLVQHMSGPLFIIVLLFIFFCLGFIVPSSSGLAVLSMPIFAPLADTV
                     GIPRFVIVTTYQFGQYAMLFLAPTGLVMATLQMLNMRYSHWFRFVWPVVAFVLIFGGG
                     LLITQVLIYS"
BASE COUNT         1630 a          684 c          891 g         1582 t
ORIGIN      
        1 tcatgtccac cgatgatagt ttgttgttta tcactatatt ttaaaaatag tctataaggc
       61 ttatcagatg aagtagctgc tggcgtaaca ccaccagtaa atgtttcatc ataagtccag
      121 taaccttcaa ctgtgtcctc atctttagtt ccaaatatat caacgtattt atttcttaac
      181 tgattaatgt ttccccaact ctcggatcca aacacttgaa tatgactata ccaaacccac
      241 gtttgcaatg ttgcatgaat gctaccattg ggcttttgcc ataaccattt atttgatagt
      301 gataaatgag gctgtgtata atactttatt aactcattga tattagtctc gttttcactg
      361 atattatagg cttttgcttc agatgaaaaa ctgattggtg ttttaggaag ttgtgttgat
      421 gtagttccta aaagtaacaa tgctgttgat aaaactaatt tattcatgat gttctttttc
      481 atatgaaaat ctcctttgcg tgaattaccc aaagtatata agctattaca ccgattcgga
      541 attaaataaa agctaaaact atgttaaata aacttaaaca gttagtagtg ttatttaagc
      601 aaaacttatc atttttaagt tttggacaga aacagtactt aataaagtag gcgggagtta
      661 tatattaaaa acgacacgtt acaattattc tctaatcaat tgcattaaat tgtttgataa
      721 ttgaattttc taactacttg aaaaatagtt atactttaaa tgtagtactt attttaatta
      781 tttcctacta cttaaattta atattaataa aatgttcatt taattattga taaaatatta
      841 caaattttaa tagtaggttg tgtttatttt gtatgcgctt acaatttagg cgtaactaaa
      901 ataaaaggag ttgttattaa tgaaaaattt acgaaacaga agttttttaa ctttattaga
      961 cttttcacga caagaggtag aattcttatt aacactctcc gaagatttaa aacgtgctaa
     1021 atatattggc actgaaaagc ctatgttaaa aaataaaaat attgcactgt tatttgaaaa
     1081 agattctaca agaacgcgat gtgcatttga agttgcagcg catgatcaag gtgcaaatgt
     1141 aacttattta ggcccaactg gatcacaaat gggtaaaaaa gaaacaacta aagatactgc
     1201 acgtgtgctt ggtggaatgt atgatggcat tgaataccgt ggtttttcac aaagaacagt
     1261 agaaacttta gctgagtatt caggcgtacc agtgtggaat ggtttaactg atgaagatca
     1321 tcctactcaa gttcttgctg atttcttaac agcaaaagaa gtcttaaaaa aagattatgc
     1381 agatattaac tttacatatg ttggagatgg tcgtaataac gttgcaaatg cattaatgca
     1441 aggtgctgcc attatgggta tgaacttcca tttagtttgt ccaaaagaat taaatccaac
     1501 agatgaatta ttaaatcgct gtaaaaatat tgccgctgaa aatggtggca acatattaat
     1561 cacagataat attgaccaag gtgtaaaagg ttcggatgta atttacactg acgtttgggt
     1621 atcaatgggt gaacctgatg aagtatggaa agaacgactt gaattattga aaccatatca
     1681 agtaaataaa gaaatgatgg ataaaactgg taatccaaat gttatttttg agcattgctt
     1741 accatctttc cataatgctg atacgaaaat tggtcaacaa atttttgaaa aatatggaat
     1801 tcgagaaatg gaagttacag atgaagtatt cgaaagtaaa gcttcagttg tattccaaga
     1861 agctgagaac agaatgcata caatcaaagc agtgatggtt gctacattgg gtgaatttta
     1921 aatgatataa ggaagtgaat atgatggcga aaatagtagt agcattaggt ggtaatgctt
     1981 taggaaaatc acctcaagaa caactcgagc ttgttaaaaa tactgcgaaa tcattagtag
     2041 gattaataac aaaaggacat gagattgtta ttagtcatgg taatggacca caggttggaa
     2101 gcattaattt gggacttaac tatgctgcag aacataacca aggtccggca tttccatttg
     2161 ctgaatgtgg cgcaatgagt caagcttaca tcggctatca attacaagaa agcttacaaa
     2221 atgaattgca ttctattgga atggataaac aagtggtaac actagtgaca caagttgaag
     2281 ttgatgaaaa tgatccggca tttaacaatc cttcaaaacc aattgggtta ttttacaaca
     2341 aagaagaagc tgaacaaatt caaaaagaaa aaggatttat atttgttgaa gatgctggaa
     2401 gaggatatag gcgcgttgtt ccttcaccac aacccatctc tattattgaa ttagagagta
     2461 ttaaaacact tattaaaaat gatacactcg ttattgctgc tggcggtgga ggtataccag
     2521 taattagaga gcaacatgat ggttttaaag gtattgatgc agttatagac aaagataaaa
     2581 caagtgcatt gttgggtgct aatattcaat gcgatcaatt gattatttta acagcaattg
     2641 attatgtata tattaatttt aacactgaaa accaacagcc tttgaaaaca acaaatgttg
     2701 atgaattaaa acgatatata gacgaaaatc aatttgcaaa aggaagtatg ttaccaaaaa
     2761 ttgaagcagc catatcattt attgaaaaca atccaaaagg aagcgtgctt ataacatcat
     2821 taaatgaatt agatgctgcc ttagagggta aagtaggtac tgtgattaaa aagtaattga
     2881 attgaaacgc ttttcaatta ctatatgtca aatgcatgat ttttattatt tatgtgcacc
     2941 ccctgaaaat aatgcctcta ttttgatgcg gggtgcactt tcttaattta tatttataaa
     3001 atctttaagg tagaaatgct aggttaaatg tcgaaggaga tgaaaccgtg gaaaatacaa
     3061 ttaatgaaag tgaaaagaaa aaacgattta aattaaaaat gccaggtgca tttatgattt
     3121 tattcatttt aacggttgtt gcagttatag caacatgggt tattcctgct ggtgcatatt
     3181 ctaaactttc ttacgaacct tcatcccaag aactaaagat agttaaccct cataaccaag
     3241 tgaaaaaggt tccgggtacg caacaggaac tagacaaaat gggagttaaa attaagattg
     3301 aacaatttaa atcaggtgca attaataagc cggtatcaat tccgaatact tatgaaagat
     3361 taaagcaaca tccagctgga ccagaacaaa taacaagtag catggttgaa ggtacgatag
     3421 aagcggtcga tatcatggta ttcattcttg tactaggggg acttattggc gtagttcaag
     3481 ccagtggttc ttttgaatcg ggattgttag ctttaacgaa gaaaacaaaa gggcatgaat
     3541 ttatgctaat tgtgtttgta tcaatactaa tgattatcgg cgggacgtta tgtggtattg
     3601 aagaagaagc tgtagcattc tatccgattt tagtccctat atttatagcg ttaggatacg
     3661 attctatcgt ttcagttggt gccatattcc ttgccagctc tgtcggtagt acattttcaa
     3721 ctattaaccc gttctcggtt gtaattgcct ctaatgccgc tggtacaact tttacggatg
     3781 gcttgtattg gagaataggt gcttgtattg tcggtgcgat ttttgttatt agttatttat
     3841 attggtactg taaaaaaatt aaaaacgatc ctaaagcgtc atattcttat gaagacaaag
     3901 atgcttttga acagcaatgg tctgtattaa aagatgacga tagtgcccat tttactttgc
     3961 gtaagaagat aatccttaca ttatttgtac taccatttcc aattatggta tggggagtta
     4021 tgacgcaagg ttggtggttc ccagttatgg cttcagcatt tttaatattt acaattataa
     4081 taatgtttat tgctgggaca ggtaaatctg gattgggaga aaaaggaact gtagatgcat
     4141 ttgtcaatgg cgcatcaagt ttagtaggtg tatctttaat tattggttta gctcgaggta
     4201 ttaatttagt gttgaatgaa ggtatgattt cagatacaat cttacacttt tcatcatctt
     4261 tagttcaaca tatgagtgga ccattattta tcatcgtatt actatttatt ttcttctgtt
     4321 taggttttat cgtgccatca tcttctggat tagcggtatt atcaatgcct atctttgcac
     4381 cactagctga tacagtaggt ataccaagat tcgtcatcgt tacgacatat caatttggtc
     4441 aatatgcgat gttattctta gcgccgactg gacttgttat ggccacacta caaatgttaa
     4501 acatgcgata ttcacattgg ttccgatttg tatggccggt agttgctttt gtattgattt
     4561 tcggtggcgg actactaatt acgcaagtac taatttattc ataatttgaa atgctatatt
     4621 ataaaaatac taaatgggtt ttatgcatct cgtaggtttg tagaaatact aaactaagcg
     4681 aggtgcatta ttatttttga ttaagaaaat aatgacggta atcataacac tagtaagtaa
     4741 ttgatacgat actttattta ataatgatat ttttaaaatt tgttttt
//
LOCUS       DAMZKC010000167         4776 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.169, whole genome shotgun sequence.
ACCESSION   DAMZKC010000167 DAMZKC010000000
VERSION     DAMZKC010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4776
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.169"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            253..672
                     /locus_tag="POB55_002256"
     CDS             253..672
                     /locus_tag="POB55_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDI6501202.1"
                     /translation="MYKQLEKLITLTNNDLNLVNRRFGQRTDITSEQLELLRILFNYD
                     RLSQYDLTMKISREQSIVSRWIKKLVLKGYITSQQSSEDLRCKELILTDQARTLISQI
                     NNARCELIEARCQCLSEVELDNLNQLLDKLNQRRISL"
     gene            699..809
                     /locus_tag="POB55_002257"
     CDS             699..809
                     /locus_tag="POB55_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501203.1"
                     /translation="MSLCTQQKLNHELQAKVAVIVKIDGKQSPLFLLVYV"
     gene            complement(879..4631)
                     /locus_tag="POB55_002258"
     CDS             complement(879..4631)
                     /locus_tag="POB55_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDI6501204.1"
                     /translation="MAKKFNYKLPSMVALTLVGSAVTAHQVQAAETTQDQTTNKNVLD
                     SNKVKATTEQAKAEVKNPTQNISGTQVYQDPAIVQPKTANNKTGNAQVSQKVDTAQVN
                     GDTRANQSATTNNTQPVAKSTSTTAPKTNTNVTNAGYSLVDDEDDNSENQINPELIKS
                     AAKPAALETQYKAAAPKAATTSAPKAKTEATPKVTTFSASAQPRSVAATPKTSLPKYK
                     PQVNSSINDYIRKNNLKAPKIEEDYTSYFPKYAYRNGVGRPEGIVVHDTANDRSTING
                     EISYMKNNYQNAFVHAFVDGDRIIETAPTDYLSWGVGAVGNPRFINVEIVHTHDYASF
                     ARSMNNYADYAATQLQYYGLKPDSAEYDGNGTVWTHYAVSKYLGGTDHADPHGYLRSH
                     NYSYDQLYDLINEKYLIKMGKVAPWGTQSTTTPTTPSKPSTGKLTVAANNGVAQIKPT
                     NSGLYTTVYDKTGKATNEVQKTFAVSKTATLGNQKFYLVQDYNSGNKFGWVKEGDVVY
                     NTAKSPVNVNQSYSIKPGTKLYTVPWGTSKQVAGSVSGSGNQTFKASKQQQIDKSIYL
                     YGSVNGKSGWVSKAYLVDTAKPTPTPTPKPSTPTTNNKLTVSSLNGVAQINAKNNGLF
                     TTVYDKTGKPTKEVQKTFAVTKEASLGGNKFYLVKDYNSPTLIGWVKQGDVIYNNAKS
                     PVNVMQTYTVKPGTKLYSVPWGTYKQEAGAVSGTGNQTFKATKQQQIDKSIYLFGTVN
                     GKSGWVSKAYLAVPAAPKKAVAQPKTAVKAYTVTKPQTTQTVSKIAQVKPNNTGIRAS
                     VYEKTAKNGAKYADRTFYVTKERAHGNETYVLLNNTSHNIPLGWFNVKDLNVQNLGKE
                     VKTTQKYTVNKSNNGLSMVPWGTKNQVILTGNNIAQGTFNATKQVSVGKDVYLYGTIN
                     NRTGWVNAKDLTAPTAVKPTTSAAKDYNYTYVIKNGNGYYYVTPNSDTAKYSLKAFNE
                     QPFAVVKEQVINGQTWYYGKLSNGKLAWIKSTDLAKELIKYNQTGMTLNQVAQIQAGL
                     QYKPQVQRVPGKWTDANFNDVKHAMDTKRLAQDPALKYQFLRLDQPQNISIDKINQFL
                     KGKGVLENQGAAFNKAAQMYGINEVYLISHALLETGNGTSQLAKGADVVNNKVVTNSN
                     TKYHNVFGIAAYDNDPLREGIKYAKQAGWDTVSKAIVGGAKFIGNSYVKAGQNTLYKM
                     RWNPAHPGTHQYATDIDWANINAKIIKGYYDKIGEVGKYFDIPQYK"
BASE COUNT         1373 a          772 c          842 g         1789 t
ORIGIN      
        1 cactagtgta gggttgtaac atatgtccaa atacgactaa taatattaaa attgctcttg
       61 cgttgtcaaa aaaataatct ctctctttta atgaagtcat attttatctc ctttatgtcc
      121 ataagactct tactaattta ttatattttg tattagttat gcaacaaatt tgtctattgt
      181 gtcgaatagt attgtaaaaa ttataaaaaa agcatataat attataaata atatgtagtg
      241 gagtagaaca acatgtataa acaacttgaa aaacttatta cactgactaa caatgactta
      301 aacttagtga atagaagatt tggacaacgc acggatatca catctgaaca gctagaactt
      361 ctccgtattt tatttaatta cgatcgctta tcacagtatg atttgacgat gaagattagc
      421 agggaacaat ctatagtttc aaggtggatt aagaaattag ttttgaaagg gtacatcaca
      481 agtcaacaat ctagcgaaga tttaagatgt aaagaattaa ttttgactga tcaagcacgt
      541 acattaattt cacaaataaa taatgcacgt tgcgaattga ttgaagcaag atgtcaatgt
      601 ttatcggaag tcgaattaga caatttaaat caattacttg ataagttaaa tcaacgacgc
      661 atatcgttgt aacacgacaa tagcaaacac ataatttagt gtcgttgtgc acacaacaaa
      721 aattgaatca tgaattacaa gcaaaagtag cggtgattgt taaaattgat ggtaaacaat
      781 caccgctatt tttgcttgtg tatgtataaa aaagggatca aaggtcatcc cccatgattg
      841 atagtggggg atgacttttg atcctatgtt catgttgctt atttatattg tgggatgtcg
      901 aagtatttgc cgacttcgcc aattttatca tagtagcctt tgatgatttt agcattgatg
      961 ttagcccaat ctatatctgt agcatattgg tgtgttcctg gatgtgcagg attccatctc
     1021 attttgtaaa gtgtattttg accagctttt acatatgagt tgccgatgaa tttagcacca
     1081 ccaacgattg cttttgatac tgtgtcccaa ccagcttgtt tagcatattt aataccttca
     1141 cgtaaaggat cgttatcata tgcagcaata ccaaatacgt tatggtattt cgtgtttgag
     1201 ttagttacaa ctttgttgtt cactacatct gcacctttcg ctaattgaga agtaccgtta
     1261 cctgtttcta atagggcatg tgagataaga taaacttcat taatgccata catttgagca
     1321 gctttgttaa atgcagcacc ttggttttct aatacacctt taccttttaa gaattgattg
     1381 attttatcaa tagaaatatt ttgtggttgg tctaagcgta agaattgata ttttaatgct
     1441 ggatcttgag ctaaacgctt cgtatccatt gcatgcttaa catcattaaa gttagcatct
     1501 gtccacttac ctggtacacg ttgtacttgt ggtttatatt gtaaaccagc ttgtatttga
     1561 gcaacttggt ttaatgtcat acctgtttga ttatacttaa ttaattcttt agctaaatca
     1621 gttgatttaa tccatgctaa tttaccgtta gataatttac catagtacca agtttgtcca
     1681 ttaatgactt gttctttaac aactgcgaat ggttgttcat taaatgcttt taatgagtat
     1741 ttagctgtat cagaatttgg tgttacatag taataaccat taccattttt aattacataa
     1801 gtgtagttat aatctttggc agctgatgta gttggtttaa cagcagttgg tgcagttaaa
     1861 tcttttgcat ttacccaacc agtgcggtta ttaatagtac cgtataaata aacatctttg
     1921 cctacagata cttgtttcgt tgcattaaat gtaccttgag caatgttatt gcctgttaaa
     1981 atgacttggt ttttagtacc ccaaggaacc attgataagc cgttatttga tttattaaca
     2041 gtatattttt gagtcgtttt aacttctttg cctaagtttt gaacatttaa gtcttttaca
     2101 ttgaaccaac ctaatgggat gttatggctt gtattgttta ataatacata cgtttcatta
     2161 ccatgagcac gctcttttgt tacatagaac gtacggtctg catatttcgc accgtttttc
     2221 gctgtttttt cataaacaga agcacgaata ccagtgttgt ttggtttaac ttgagcaatc
     2281 ttgctaactg tttgagtcgt ttgtggttta gtaacagtat aagcttttac agctgttttt
     2341 ggttgtgcta ctgctttttt aggtgcagca ggtacagcta aatatgcttt acttacccaa
     2401 ccagatttac catttacagt tccaaataaa tagatagatt tatcaatttg ttgttgctta
     2461 gtcgctttaa aagtttggtt acctgtacca gaaactgcac cagcttcttg tttataagtg
     2521 ccccaaggta ctgaatataa tttagtgcct ggttttactg tatatgtttg cattacattt
     2581 acaggtgatt ttgcattgtt ataaataacg tcaccttgtt taacccaacc aattaaagtt
     2641 ggactattgt aatctttaac taagtagaat ttgtttccac ctaaacttgc ttcttttgtt
     2701 acagcaaatg ttttttgaac ttctttcgtt ggcttaccag ttttgtcata aactgtagtg
     2761 aataagccat tgtttttagc attaatttga gcaacaccgt ttaatgatga aactgttaat
     2821 ttattatttg ttgtaggtgt tgatggctta ggtgttggtg taggcgtagg tttagcagta
     2881 tcaactaaat atgctttact tacccaacca gatttaccat tcacagagcc atataaataa
     2941 attgatttat caatttgttg ttgctttgaa gccttaaatg tttggtttcc agagccagac
     3001 acactaccag caacttgttt agatgtaccc caaggtactg tataaagttt cgtaccaggt
     3061 ttgattgaat atgattgatt tacatttaca ggtgatttag ctgtgttgta aaccacatcg
     3121 ccttctttaa cccaaccaaa tttattacca gaattgtaat cttgaacaag atagaatttt
     3181 tgattaccta atgtagctgt tttagataca gcaaatgttt tttgaacttc attagttgct
     3241 ttaccagttt tgtcgtatac agtagtatat aaaccactat ttgttggttt gatttgtgcg
     3301 acaccattgt ttgcagcaac tgttaattta ccagttgatg gtttcgacgg tgtagtaggg
     3361 gtagttgtag attgcgtacc ccatggcgcc actttaccca tttttattaa atatttttca
     3421 ttaattaagt catataattg atcataacta taattatgac ttcttaaata tccatgtgga
     3481 tcggcatggt cagtaccacc taaatattta cttacagcgt agtgagtcca tactgtacca
     3541 tttccatcat actcagcact gtctggtttt aaaccataat attgtaattg tgtagctgca
     3601 tagtcagcat agttattcat tgaacgtgca aatgaagcat agtcgtgtgt gtgtacgatt
     3661 tcaacattga tgaatctagg gttaccgact gcaccgacac cccaagataa gtaatccgtt
     3721 ggtgctgttt cgattatacg atccccatca acaaatgcat gtacgaatgc gttttgatag
     3781 ttatttttca tataactaat ttcaccattt atcgtcgaac gatcattagc tgtatcatga
     3841 actacgatac cttcaggacg acctacgccg ttacggtatg cgtatttagg gaagtaagat
     3901 gtataatctt cttcaatttt aggtgctttt aagttatttt tacgaatgta atcgttaatt
     3961 gaagagttta cttgtggttt atattttggc aaactcgttt ttggtgttgc agcaactgat
     4021 cttggttgtg ctgaagcgct aaaagtagtt actttaggtg tcgcttcagt tttagcttta
     4081 ggtgctgatg tagttgcagc tttaggtgct gcggctttat attgcgtttc aagagctgca
     4141 ggtttagcag ctgatttaat taattctgga ttaatttgat tttctgaatt atcatcttca
     4201 tcatcaacta aactataacc agcatttgta acattagtgt tagttttagg tgctgtagtg
     4261 cttgttgact ttgcaacagg ctgcgtatta tttgtagtcg ctgattgatt agcacgagtg
     4321 tcaccattta cttgtgcagt atcaactttt tgacttactt gagcattgcc tgttttgtta
     4381 tttgctgttt ttggttggac aatagcaggg tcttgatata cttgagtgcc agaaatgttt
     4441 tgcgttggat tttttacctc agcttttgct tgttcagtag ttgctttaac tttattacta
     4501 tctaaaacgt ttttattagt agtttgatct tgtgtcgtct cagctgcttg aacttgatgt
     4561 gcagtgactg ctgaacctac aagcgttaat gcaaccattg atggtagttt gtaattgaat
     4621 tttttcgcca ttctatttat tactcctaac atttattaat tattactaac attatagtac
     4681 ctgttttata tacctgtgcg tatttaacca atttgtaata tactcttaat ttacattttc
     4741 actgtaaaaa aatgaaaaaa attttgaatc tcaatg
//
LOCUS       DAMZKC010000168         4711 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.170, whole genome shotgun sequence.
ACCESSION   DAMZKC010000168 DAMZKC010000000
VERSION     DAMZKC010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4711
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.170"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(211..732)
                     /locus_tag="POB55_002259"
     CDS             complement(211..732)
                     /locus_tag="POB55_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwhD family protein"
                     /protein_id="HDI6501205.1"
                     /translation="MEVISLSEKKGFNFNIIKNDPLDGHKGTNIGSISLDNIAPVFID
                     VANKEAFIDIGGMHARAKVEKGVKWITDKAAVEGDEAKEYWLCWVTTERNEQGPYYAG
                     LTACYLLVNKAIRRGYKSMPEHVNMMDKSMKHHIIIDQIGDENKAILKDFLMNHDEGM
                     WKHSSDALHQAFN"
     gene            complement(760..2055)
                     /locus_tag="POB55_002260"
     CDS             complement(760..2055)
                     /locus_tag="POB55_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDI6501206.1"
                     /translation="MTNAYVDLKLVEEKVFKDPIHRYIHVEDQLIWDLIKTKEFQRLR
                     RIRQLGTLYLSFHTAEHSRFGHSLGVYEIVRRLIDESFIGHDAWDNKDRPLALCAALL
                     HDLGHGPFSHSFEKIFNTDHEAYTQAIITGDTEVNAVLRKVAPEFPREVAEVINKTHH
                     NKLVISMISSQIDADRMDYLQRDAYFTGVSYGAFDMERILRLMRPSKDEVLIKESGMH
                     AVENFIMSRYQMYWQIYFHPVSRGGEVLLNNCLKRAKQLYNEGYEFKLHPHDFIPFFE
                     ETVTIEQYVELDEAVVTYYLEKWTKEDDAILSDLASRFINRDLFKYIPFDGSIITISE
                     LQELFEAGGINPDYYFVSEAFSDLPYDYDRPGSNRKPIHLLRQDGTIREISNQSLVIH
                     SITGINRQDYKLYYPREMVAKIKDKTIREAIENLINELN"
     gene            complement(2095..2571)
                     /locus_tag="POB55_002261"
     CDS             complement(2095..2571)
                     /locus_tag="POB55_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6501207.1"
                     /translation="MFKVRQATEKDVVQIRDVATKAWFNTYLNIYAATTVNHLLEASY
                     NEHHLKKRLQEQLFLVVEEGNDIVGFANFIYGEELYLSAHYVKPESQHTGYGTALLNE
                     GLSRFEDKFEGVYLEVDNKNEEAVAYYKEQGFTILRSYEPEMYGEKLDLALMYKAF"
     gene            complement(2740..3276)
                     /locus_tag="POB55_002262"
     CDS             complement(2740..3276)
                     /locus_tag="POB55_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDI6501208.1"
                     /translation="MITVLFGGSRPNGNTAQLTKFALQDLEYQWIDVTQHQFKPIRDV
                     RHTAETITSYDDDYLPILDKILASDTIIFASPVYWYSISAPLKAFIEHWSETLQDKRY
                     PNFKAQMAEKDFRVILVGGDCPKIKAKPAITQMKYSLDFLGATLNGYIIGTAEKPGDI
                     MKDNYALARATEWNSILQ"
     gene            complement(3487..4425)
                     /locus_tag="POB55_002263"
     CDS             complement(3487..4425)
                     /locus_tag="POB55_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDI6501209.1"
                     /translation="MKQFVNLGKSDVEVFPIALGTNAVGGHNLYPNLDEEQGKDVVRQ
                     AINHGINLLDTAYIYGPERSEELVGEVVKEYPREQIKIATKGSHEFDENQEVHQNNQP
                     EYLKQQVENSLKRLQTDYIDLYYIHFPDNNTPKDQAVAALQELKEQGKIKAIGVSNFT
                     LDQLKEANKDGYVDVVQLEYNLLHRENEAVLQYCVDHQITFIPYFPLASGILAGKYDE
                     NTKFSDHRTTRRDFKPGVFEENVRRVKALESIAAAHQTSIANIVLAFYLTRPAIDVII
                     PGAKRAEQVVENIKAADIVLSDDEIQYIDELFPIED"
BASE COUNT         1499 a          887 c          632 g         1693 t
ORIGIN      
        1 atgtgaatta attcacaaag tatatagcta tgatcaaaaa aatgtgtcag ttattcaaat
       61 tttttattaa ctacagatta atttaatttc aaaaaaatta aaaaaactcc caatcaatct
      121 aagactttat tttcacctca agatttacgt cgtcattgag aaatgaatca tatctttata
      181 gattaattgg gaaattttag tttctaatat ttaattaaat gcttgatgta aagcatcaga
      241 agaatgcttc cacatacctt catcatggtt cattaaaaag tcttttaaaa tagctttatt
      301 ctcgtcacca atttgatcaa tgataatatg atgtttcatt gatttatcca tcatattaac
      361 atgttcaggc atacttttat aaccacgacg aattgcttta ttcactaata aatagcacgc
      421 tgttaaacca gcgtaatatg gtccttgttc attacgttct gttgttaccc aacacaacca
      481 atattcttta gcttcatcgc cttcaacagc agctttatca gtaatccatt tcacaccttt
      541 ttcaactttg gcacgagcat gcatgcctcc aatatcaata aatgcttctt tgttagcaac
      601 atcgataaaa actggtgcaa tattgtctaa gctaattgaa ccaatatttg tacctttatg
      661 accatctaga gggtcatttt ttatgatatt aaaattaaag ccttttttct cagacaatga
      721 tataacctcc atatttaacg ataacaattt tagccctgtt taattaagct cattaatcaa
      781 attttcaata gcttctctaa ttgtcttatc tttaatcttt gcaaccattt ctctaggata
      841 atataattta tagtcttggc gattaatgcc tgtaatacta tgaatgacta atgattgatt
      901 gcttatttct ctaatcgtac catcttgtct taataaatga atcggtttgc gatttgaccc
      961 cggtcgatca tagtcatatg gcaaatccga aaatgcttca ctcacaaaat aataatctgg
     1021 attaatacca cctgcttcaa acagttcttg cagttctgat attgtaataa ttgagccatc
     1081 aaatggaata tatttaaata agtctcgatt aataaatcga cttgctaaat cacttaaaat
     1141 ggcatcatct tcttttgtcc atttttccaa ataatacgta actaccgctt catcgagttc
     1201 aacatattgt tcaatcgtaa ctgtctcttc aaaaaatgga ataaaatcat gtggatgcaa
     1261 cttaaattca tagccttcat tataaagctg ttttgcgcgt ttcaaacaat tattaagcag
     1321 cacttctcca ccacgactaa ctgggtggaa gtaaatttgc caatacattt gataacgact
     1381 cataataaag ttttcaactg catgcatacc actttctttg attagtactt catctttaga
     1441 aggtcgcatt aatcttaaaa tacgctccat atcaaaagca ccatatgata cacctgtgaa
     1501 atacgcatca cgttgtaaat aatccattct atccgcatcg atttgtgacg aaatcatcga
     1561 aatgaccaat ttattatgat gcgttttatt aattacttcc gcaacttctc ttggaaactc
     1621 aggcgccact ttacgtaata cagcattcac ctcagtatct ccagtaataa tcgcttgtgt
     1681 gtatgcttca tggtctgtat taaatatttt ttcaaaacta tgtgaaaatg gaccatgtcc
     1741 taaatcatgt aataatgcag cacataatgc caacggtcta tctttattgt cccatgcatc
     1801 atgaccaata aatgactcat caattaatcg tctaactatt tcatacacac ctaaagaatg
     1861 tccaaagcga ctatgttctg ctgtgtgaaa agataggtac agtgttccta gttgtctaat
     1921 tcgacgtaac ctttggaatt ccttagtttt aattaaatcc catatcaatt gatcttcaac
     1981 atgaatatat cgatgtatcg ggtctttaaa aactttttct tctactaatt ttaaatctac
     2041 atatgcgtta gtcattattc ccctcctttt cgtttaatat aatatttaat ttacttaaaa
     2101 tgctttgtac ataagtgcta agtctaactt ttcgccatac atttctggct cataagagcg
     2161 taagattgta aaaccttgct ctttatagta agctactgct tcttcatttt tattatctac
     2221 ttctaagtaa acaccttcaa atttatcttc aaaacgtgat aatccttcat ttaacaatgc
     2281 tgtaccataa cctgtatgtt gcgattctgg tttaacataa tgagctgata aatataattc
     2341 ttcaccgtaa ataaagttag caaagccaac gatgtcatta ccttcttcaa cgactaagaa
     2401 taattgttct tgaagtcttt tctttaaatg atgttcatta tatgaagctt ctaacaagtg
     2461 attaactgtt gtcgcagcgt atatatttaa gtatgtatta aaccaagctt tagttgcgac
     2521 atctctaatt tgaacaacat ctttttcagt tgcttgtctt accttgaaca tgactttctc
     2581 cccttattaa caagttttaa taacggcatt ataccacaac ttgctcaata cttaataaac
     2641 aatgattgtc tattcaattt atatatttat attttccgtt aaaattaaaa ataaaaaata
     2701 acgaagcaaa aaatcacttc gtttagtatg aggtatgtct tattgcaata tactattcca
     2761 ctcagttgca cgtgctaagg catagttgtc tttcatgatg tcaccaggct tttcagcagt
     2821 tccaataata taaccattta aagtggcacc taaaaagtct aaactatatt tcatttgcgt
     2881 aattgctggc ttcgctttta tttttggaca atctccacca actaaaataa ctctaaaatc
     2941 cttttcggcc atttgtgcct taaaattagg atatcgttta tcttgtaatg tttctgacca
     3001 atgttcgata aatgctttca atggtgctga aatgctatac caatacactg gtgatgcaaa
     3061 aataattgta tcactagcca atattttatc tagaatcggc aaatagtcat cgtcatatga
     3121 agtaatagtc tctgctgtat gtctcacgtc acgtatcggt ttaaactgat gttgtgtcac
     3181 gtcaatccat tgatactcta aatcttgcaa agcgaatttt gttaattgtg cagtattacc
     3241 gtttggtcta ctcccaccaa acaaaacagt aatcatttta gcctaacctc acttttgatt
     3301 aataaatatc tgtgtttttc gttacctaat tatactatca taagctttgc ctaccgaata
     3361 gtaaaacgct tacaactttt atataaattt gacgaaattt cgtcatgcct tatataacgt
     3421 cgtttgtgat acggggctaa ttcatgatga aattagatac atatatcacc attaaataca
     3481 attcatttag tcttcaatcg gaaacagttc atcgatatat tgaatctcat catctgataa
     3541 aacgatatct gcagctttaa tattttcaac gacttgttct gcacgttttg caccaggaat
     3601 aatcacatcg atagctggtc tcgttaaata aaatgctaat acaatgttcg caattgaagt
     3661 ttgatgtgct gcagctatgc tttccaaagc ttttacgcga cgcacatttt cttcaaatac
     3721 acctggttta aaatcacgac gtgtagtacg atggtcacta aatttagtgt tctcatcata
     3781 ttttccagct aaaataccgg atgctaatgg gaaatatgga ataaatgtga tttggtgatc
     3841 aacacaatat tgtaatactg cctcattttc gcgatgcaat aaattatatt ctaactgtac
     3901 aacatcaacg taaccatctt tatttgcttc tttaagttga tctaatgtga aatttgatac
     3961 accaattgct ttaatcttcc cttgttcctt aagctcttgt aatgctgcaa ctgcttgatc
     4021 tttcggagtg ttgttatccg gaaaatgaat ataatataaa tcgatataat cagtttgtag
     4081 acgtttcaaa ctattctcaa cttgttgttt taaatattcc ggttgattgt tctgatgtac
     4141 ttcttgattt tcatcaaatt catgagaccc tttcgtagca attttaattt gctctcgcgg
     4201 atattcttta acaacttctc caaccaattc ttctgatcgt tctggcccat aaatatatgc
     4261 cgtatctaat aaattaatac catgattaat ggcttgacga acaacatctt ttccttgttc
     4321 ttcatctaag ttcggatata aattatgccc acctactgcg ttcgtcccaa gtgcgattgg
     4381 aaacacttca acatcagatt tacctaagtt tacaaattgc ttcattagac ccagctcctt
     4441 atctttataa cttttccact atcacactat catgatttaa tacaacttcc aaatgttaga
     4501 attagagctt gaatttaagc ggtgtaatag gaaaacgtta tataaaactc agttaaaata
     4561 agtattgctt aaattgatat gcatatagcg cgattgctcg ttgaccttta aattacatgt
     4621 acttttgaat cagaaattga aaaacattta agaacgacaa tattaaagcc attgcaaatt
     4681 taatttggta atttctattg gtttttaagt a
//
LOCUS       DAMZKC010000169         4694 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.171, whole genome shotgun sequence.
ACCESSION   DAMZKC010000169 DAMZKC010000000
VERSION     DAMZKC010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4694
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.171"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            3..1292
                     /locus_tag="POB55_002264"
     CDS             3..1292
                     /locus_tag="POB55_002264"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HDI6501210.1"
                     /translation="MNFSESERLQQLSNEYILGGVNSPSRSYKAVGGGAPVVMKEGHG
                     AYLYDVDGNKFIDYLQAYGPIITGHAHPHITKAIQEQAAKGVLFGTPTELEIEFSKKL
                     RDAIPSLEKIRFVNSGTEAVMTTIRVARAYTKRNKIIKFAGSYHGHSDLVLVAAGSGP
                     SQLGSPDSAGVPESVAREVITVPFNDINAYKEAIEFWGDEIAAVLVEPIVGNFGMVMP
                     QPGFLEEVNEISHNNGTLVIYDEVITAFRFHYGAAQDLLGVIPDLTAFGKIVGGGLPI
                     GGYGGRQDIMEQVAPLGPAYQAGTMAGNPLSMKAGIALLEVLEQDGVYEKLDSLGQQL
                     EEGLLKLIEKHNITATINRIYGSLTLYFTDEKVTHYDQVEHSDGEAFGKFFKLMLNQG
                     INLAPSKFEAWFLTTEHTEEDIQQTLKAADYAFSQMK"
     gene            1585..2679
                     /locus_tag="POB55_002265"
     CDS             1585..2679
                     /locus_tag="POB55_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014667727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HDI6501211.1"
                     /translation="MRLGARIFKTGIAIILAMSIASLLPDDVGLKALAGVSAVVAMQP
                     SIYRSFKTVSDQALGNIIGAILSVTMVTIFSDNFIIMGVTVIVLIAILFKFNLAHVAT
                     LASVTALIIMGQHTGSFYITAFYRFVLVMIGVISSSLVNFVFLPPKFETKIYYNSLNI
                     SSDIFMWFKLVLNDTTEFNNIKQDSHNLKQRVEKLEKIYDYYSEERPITKKHIHQQNR
                     KKILFREVVQTTRQAYEVLNKLSRYQNDLYLLNNNFLLQIKLDLDSLTAFHEQILASL
                     SKKARYNVTHVDYELDNPQKKDLLSTFQHELINHPYQTEYSFANVMQIVAAIEEYRHH
                     LEHLDRIRISFFTYHRSDADIEIVEEDFDL"
     gene            complement(2769..2862)
                     /locus_tag="POB55_002266"
                     /pseudo
     CDS             complement(2769..2862)
                     /locus_tag="POB55_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500499.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2871..4607)
                     /locus_tag="POB55_002267"
     CDS             complement(2871..4607)
                     /locus_tag="POB55_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAV1866 family putative multidrug efflux ABC
                     transporter"
                     /protein_id="HDI6501212.1"
                     /translation="MIKRYLQFVKPYKYRIFATIIVGIIKFGIPMLIPLLIKYAIDGV
                     INNHALTTDEKVHHLTIAIGIALFIFVIVRPPIEFIRQYLAQWTSNKILYDIRKKLYN
                     HLQALSARFYANNQVGQVISRVINDVEQTKDFILTGLMNIWLDCITIIIALSIMFFLD
                     VKLTLAALFIFPFYILTVYVFFGRLRKLTRERSQALAEVQGFLHERVQGISVVKSFAI
                     EDNEAKNFDKKNTNFLTRALKHTRWNAYSFAAINTVTDIGPIIVIGVGAYLAISGSIT
                     VGTLAAFVGYLELLFGPLRRLVASFTTLTQSFASMDRVFQLIDEDYDIKNGVGAQPIE
                     IKQGRIDIDHVSFQYNDNEAPILKDINLSIEKGETVAFVGMSGGGKSTLINLIPRFYD
                     VTSGQILIDGHNIKDFLTGSLRNQIGLVQQDNILFSDTVKENILLGRPTATDEEVVEA
                     AKMANAHDFIMNLPQGYDTEVGERGVKLSGGQKQRLSIARIFLNNPPILILDEATSAL
                     DLESESIIQEALDVLSKDRTTLIVAHRLSTITHADKIVVIENGHIVETGTHRELIAKQ
                     GAYEHLYSIQNL"
BASE COUNT         1576 a          792 c          740 g         1586 t
ORIGIN      
        1 aaatgaattt tagtgaaagt gaacgtttac aacaactttc aaacgaatat attctaggcg
       61 gtgtcaattc cccttctcgt tcttataaag ctgtaggagg cggtgcacct gttgttatga
      121 aagaaggaca cggtgcatat ttatatgatg tcgatggcaa taaatttatt gattaccttc
      181 aagcatacgg tccaattatt acggggcatg cacatcctca tattactaaa gcaattcaag
      241 aacaagctgc taaaggtgtt ttatttggta caccgactga attagaaatt gaattcagca
      301 aaaaattacg tgatgcaatt ccatctcttg agaaaattcg ctttgtaaat tctggaacag
      361 aagcagtcat gacaacaatt cgtgttgcac gtgcatatac taaaagaaat aaaattataa
      421 aatttgctgg atcttatcat ggccattctg atttagtatt ggttgcagca ggtagcggcc
      481 catctcagct cggttctcca gactcagctg gtgttccaga aagcgtcgca cgtgaagtca
      541 ttactgtacc tttcaatgat attaacgcct ataaagaagc aattgaattt tggggtgatg
      601 aaattgccgc agtattagta gaaccaattg ttggtaactt tggaatggta atgcctcaac
      661 ctggattttt agaagaggtt aatgaaattt cacataacaa tgggacccta gtgatttatg
      721 atgaagtaat tactgcattc cgtttccatt acggtgccgc tcaagattta ttaggtgtta
      781 tccctgattt aactgcattt ggtaaaattg ttggcggtgg tttaccaatt ggaggctatg
      841 gtggacgtca agatattatg gaacaagtag cacctctagg acctgcatat caagctggta
      901 caatggctgg taacccgtta tctatgaaag caggtattgc attactcgaa gtactagaac
      961 aagacggtgt ttatgaaaaa ttagacagct taggccaaca actagaagaa ggtttactta
     1021 aattaatcga aaaacataat atcacagcta caattaatcg tatttatgga tctttaacat
     1081 tgtactttac agatgaaaaa gtcacacatt atgatcaagt tgaacattct gacggcgaag
     1141 cgttcggtaa atttttcaaa ttaatgttaa atcaaggtat caatttagca ccttctaagt
     1201 ttgaagcttg gttcttaaca actgaacata cagaagaaga tattcaacaa actttaaaag
     1261 ctgcagacta tgcttttagt caaatgaaat aacttttaag gaagtatatt tttaattgaa
     1321 ttaaaataaa acaaatgctc acatagtaga ttcattttat atcgttaact attaacgttt
     1381 aacttgatat gtcactatgt gagtttttat tttacaaatt tatactcttt taatctctga
     1441 ctaaagtttt acatcgctat cagtattatg tatgatttat tttaacaact ataaataagc
     1501 ttgaattttg taactagtaa gtgtataaca gtaatgaata cgaatattag atattcaata
     1561 aacttaaaag gggcgtcatt atttttgaga ctaggagctc ggatttttaa aactggtata
     1621 gccattattt tagctatgtc tatcgcttct ttactaccgg atgatgttgg tctgaaagcc
     1681 ttagctggtg tcagtgctgt tgttgcaatg caacctagta tttatcgttc atttaaaaca
     1741 gtttctgatc aggcattagg taacattata ggtgccatac tttctgttac aatggtaaca
     1801 atttttagtg ataattttat cattatgggt gttaccgtca tagtacttat tgctatttta
     1861 tttaaattta atttagcaca tgttgctact ttggcgagtg taacagcatt aatcattatg
     1921 ggacaacata ccggttcttt ctatattacc gccttttaca ggtttgtact tgtaatgatt
     1981 ggtgtcataa gttcatctct agttaacttt gtgtttctac cacctaaatt tgaaacaaaa
     2041 atttactata attcattaaa tatatcctca gatattttta tgtggtttaa attagtatta
     2101 aatgatacga cggagtttaa taatattaaa caagatagtc ataatttaaa acaacgtgtc
     2161 gaaaagttag agaaaattta tgattactat agcgaagaac gtcctattac aaaaaaacat
     2221 attcaccaac agaatagaaa gaaaatactt ttcagagaag tagttcagac gactagacaa
     2281 gcttatgaag tattaaacaa attgtcgcgt tatcaaaacg acttatattt acttaataac
     2341 aatttcctat tacaaatcaa attagattta gattcattaa ctgcttttca cgagcaaatt
     2401 ttagctagcc tttctaaaaa agcacgttat aatgtgacac atgttgatta tgagttagat
     2461 aatccgcaga aaaaagattt attgtctaca tttcagcatg agttaataaa tcatccttat
     2521 caaacggaat attcgtttgc caatgttatg caaattgttg cagccatcga agagtatcga
     2581 caccatttag agcatttaga ccgcataaga attagctttt tcacatatca tagatctgat
     2641 gcagacattg aaattgttga agaagacttt gatttataac attaaaaaaa ttcctataat
     2701 taattccaca atgagataaa gtaatattga atctcgaaat taaatttaaa aaagcagtaa
     2761 gattattttc aattaagaaa atctcttact gctttttctt atgttatgac cccaccatcc
     2821 tgttacaaat tttctcttca ggattgtact gaagtcaaac aactaatact ttataagttt
     2881 tgaatgctat ataaatgctc gtaagcacct tgttttgcaa tcaattcacg atgcgtacct
     2941 gtttcaacaa tatgtccatt ttcaattacg acaattttgt cagcatgtgt aatagtggac
     3001 aagcgatgcg ctacgataag tgtcgttcga tctttactca acacatctaa tgcttcttga
     3061 ataatggatt cactttctaa atcaagtgca cttgttgctt catccaagat aagaattggc
     3121 ggattattta aaaatattct agcaatcgat aatctttgtt tttgaccacc tgataattta
     3181 acacctcgtt cacctacttc agtgtcatat ccctgtggca agttcataat aaagtcatgt
     3241 gcattagcca ttttcgccgc ttcaactact tcttcatctg ttgctgttgg acgaccaagt
     3301 aaaatatttt ccttaactgt atcagagaat aaaatattat cctgttgcac caatcctatt
     3361 tgatttctta aacttcccgt taaaaaatct ttaatgttgt gaccatctat taaaatttgc
     3421 ccagaagtta catcgtaaaa tctcggtatt aagttaatta atgttgattt accaccacca
     3481 ctcataccta cgaaagcaac tgtttctcct ttttcaatac tcaaattaat atcttttaaa
     3541 attggagctt cgttatcgtt atattgaaaa ctaacatgat caatatcaat acgaccttgt
     3601 ttaatttcaa taggttgagc accaacacca tttttgatgt cataatcttc atcaattaat
     3661 tggaatacac ggtccattga agcaaaactt tgcgttaaag ttgtaaatga tgcgactaaa
     3721 cgacgtaaag gcccgaacaa taactccaag tatccaacaa atgctgcaag tgtacctact
     3781 gtgattgatc cagaaatagc aagatatgca ccaacaccga tgacaattat tggtccaata
     3841 tctgtaactg tattaattgc ggcaaaggaa taggcattcc atcttgtatg tttcaaagca
     3901 cgtgttaaga aattagtgtt ctttttatca aagtttttcg cttcattgtc ttcaatcgca
     3961 aaacttttaa cgactgaaat accttgaaca cgttcatgca agaatccttg aacctcagct
     4021 aatgcttgcg atctttcacg cgtcaatttt cttaatcttc caaagaaaac gtacaccgtt
     4081 aaaatgtaaa atgggaagat aaacagtgct gctaaagtca atttcacatc taaaaagaac
     4141 attatggata gtgcaataat aattgttata caatctaacc aaatattcat taacccggtt
     4201 aaaatgaaat cttttgtttg ttcaacatca ttaatcactc tagatattac ttgacctact
     4261 tgattattag cataaaatct cgcacttaaa gcttgtaaat ggttgtataa ctttttacgt
     4321 atatcataca atattttatt acttgtccat tgcgccaaat attgacgtat aaattcaatt
     4381 ggtggtctca ctattacaaa aataaataat gcgataccaa tggcaatagt taaatgatga
     4441 actttttcat cagtcgttag tgcgtggtta ttaatcacgc catctattgc atatttaatt
     4501 aaaagtggta taagcattgg tataccaaac ttaattatcc caacaataat cgttgcaaaa
     4561 atacgatatt tgtatggctt aacaaattgc aaatatcgtt taatcataca atccccccta
     4621 atctattgcc ctatcctatt cataagcata aaaatgaata gaggttggat acataatttg
     4681 tagatgtaaa ttct
//
LOCUS       DAMZKC010000170         4679 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.172, whole genome shotgun sequence.
ACCESSION   DAMZKC010000170 DAMZKC010000000
VERSION     DAMZKC010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4679
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.172"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            96..728
                     /locus_tag="POB55_002268"
     CDS             96..728
                     /locus_tag="POB55_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6501213.1"
                     /translation="MNHLKTYQSEDYYIHDKQFVIEGPLTYEDLKALTFDAHLTAFRD
                     AEDQYEALLEITTLPEGRIYVARQDQLIVGYVTFHYPDEIERWSTGNLPYLIELGAIE
                     VSINFRQLHLAEKLIQLSLSTPEFEDYIVITTEYYWHWDLKNSKLDVFDYKKLMQRLM
                     ATGGLEIFATDDPEITSHPANCLMARIGKNITLEQQQAFDDIRYMNRFFF"
     gene            753..1922
                     /locus_tag="POB55_002269"
     CDS             753..1922
                     /locus_tag="POB55_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HDI6501214.1"
                     /translation="MQQHSSKTAYVYSDKLLQYRFHDQHPFNQMRLKLTTELLLNANL
                     LSPEQIVQPRIATDDELMLIHKYDYVEAIKHASHGIISEDEAKKYGLNDEENSQFKHM
                     HRHSATIVGGALTLADLIMSGKVLNGCHLGGGLHHAQPGRASGFCIYNDIAITAQYLA
                     KEYNQRVLIIDTDAHHGDGTQWSFYADNHVTTYSIHETGKFLFPGSGHYTERGEDIGY
                     GHTVNVPLEPYTEDASFLECFKLTVEPVVKSFKPDIILSVNGVDIHYRDPLTHLNCTL
                     HSLYEIPYFVKYLADSYTNGKVIMFGGGGYNIWRVVPRAWSHVFLSLIDQPIQSGYLP
                     LEWINKWKHYSSELLPKRWEDRLNDYTYVPRTKEISEKNKKLALHIASWYESTRQ"
     gene            complement(2020..3009)
                     /gene="ccpA"
                     /locus_tag="POB55_002270"
     CDS             complement(2020..3009)
                     /gene="ccpA"
                     /locus_tag="POB55_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HDI6501215.1"
                     /translation="MTVTIYDVAREARVSMATVSRVVNGNQNVKAETKNKVNEVIKRL
                     NYRPNAVARGLASKKTTTVGVIIPDISNIYYSQLARGLEDIATMYKYHSIISNSDNDP
                     EKEKEIFNNLLSKQVDGIIFLGGTITEEMKELINQSSVPVVVSGTNGKDAHIASVNID
                     FTEAAKEITGELIEKGAKSFALVGGEHSKKAQEDVLEGLTEVLNKNGLQLGDTLNCSG
                     AESYKEGVKAFAKMKGNLPDAILCISDEEAIGIMHSAMDAGIKVPEELQIISFNNTRL
                     VEMVRPQLSSVIQPLYDIGAVGMRLLTKYMNDEKIEEPNVVLPHRIEYRGTTK"
     gene            complement(3082..3195)
                     /locus_tag="POB55_002271"
     CDS             complement(3082..3195)
                     /locus_tag="POB55_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501216.1"
                     /translation="MKKVYIKLKYIVRYHLAFVFISSFSLNFSKQKYVTVN"
     gene            complement(3549..4640)
                     /locus_tag="POB55_002272"
     CDS             complement(3549..4640)
                     /locus_tag="POB55_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase"
                     /protein_id="HDI6501217.1"
                     /translation="MSNKLESYRSEIVSLNHQILDLLSKRGELAQKIGEEKLKQGTRI
                     YDPQREKEMLNDLIDSNKGPFNDNTIKQLFKEIFKASTDLQKSENEKHLYVSRKLKPE
                     DTIVTFDNGGIIGDGNKSFVFGPCSVESFEQVEAVAKNLHAKGEKFIRGGAFKPRTSP
                     YDFQGLGVEGLKILKQIKDKYDLNVVSEIVNPNDFEVADEYLDVFQIGARNMQNFELL
                     KEAGRTKKPILLKRGLSATIEEFVYAAEYIASQGNQNIILCERGIRTYEKATRNTLDI
                     SAVPILKQGTHLPVMVDVTHSTGRKDIMLPTAKAALAVGADGVMAEVHPDPSVALSDA
                     GQQMDLDEFQAFYDELKPLADLYNAKKLK"
BASE COUNT         1505 a          806 c          713 g         1655 t
ORIGIN      
        1 atttttactt ttatcctttt tctagtatta attattgtaa attttctata taatgaattt
       61 aatacaatca cgacgtcgat tgaggagttg aaaatatgaa tcatttaaag acgtatcaat
      121 ccgaagatta ttacattcat gacaagcaat ttgttattga aggtccttta acatacgaag
      181 atttgaaagc gcttactttc gatgcgcatt taaccgcatt tagagatgct gaagatcagt
      241 atgaagcttt gttagaaatt acaacattac cagaaggtag aatttatgtt gctcgccaag
      301 atcaactcat tgtgggttat gtcactttcc actatcctga tgaaattgag cgctggtcta
      361 caggtaacct tccatattta atcgaattgg gggcaattga agtcagcatc aattttaggc
      421 aattacatct tgcagaaaag ctaatacaac ttagcctttc tacaccagaa ttcgaggatt
      481 atatcgttat aactactgaa tattactggc attgggattt aaaaaattca aagttagatg
      541 tatttgacta taaaaaatta atgcagcggt taatggcaac tggtggactt gaaatattcg
      601 ctacagatga tccagaaata acaagtcatc cagctaattg tttaatggca agaattggca
      661 aaaatattac attagaacag caacaagcgt ttgatgatat tcgttatatg aatcggtttt
      721 tcttttaatt tttggaatat cggagggatt atatgcaaca acattcatca aaaactgcat
      781 atgtttattc agataagtta ttacaatatc gatttcatga ccaacatccc ttcaatcaaa
      841 tgcgtttaaa attaacaaca gagctacttt tgaatgcaaa tttattgtct ccagaacaaa
      901 tagtacaacc tagaattgca acagatgacg aattaatgtt aattcataaa tatgattacg
      961 tcgaagcaat taagcatgct tcacatggca ttatcagtga agatgaggct aagaaatatg
     1021 gattaaatga tgaagagaat agtcaattta agcatatgca ccgccatagt gccacaattg
     1081 ttggaggcgc tttaacttta gcagatctta ttatgtcagg caaagtatta aatggttgtc
     1141 acttaggtgg tggattgcat cacgctcaac ctggtcgagc tagtggtttt tgtatataca
     1201 atgatattgc aattaccgca caatacttag ctaaagaata caatcaacgc gttttaatca
     1261 tagataccga tgcacatcat ggagatggta cacaatggag tttctatgcc gataaccatg
     1321 ttactactta ttctatccat gaaaccggaa aatttctttt cccaggctct ggtcactata
     1381 ctgagcgcgg tgaagatatc ggctatggac acactgtaaa tgtcccactt gaaccgtata
     1441 cagaagatgc atcatttttg gagtgtttta aattaacagt tgagcctgtc gtaaagagtt
     1501 ttaaacctga tattattcta agcgtaaatg gtgtcgatat acattatcgt gatccactaa
     1561 ctcatctaaa ttgtacgtta cattcattat atgaaattcc atattttgta aaatatttag
     1621 ctgattctta tacgaatgga aaggtaatta tgtttggtgg cggaggctac aatatttgga
     1681 gagtcgtacc acgtgcatgg agtcatgtat tcttaagttt aattgatcaa ccaattcaaa
     1741 gtggttattt accgttagaa tggattaata aatggaaaca ttattcatct gaattattac
     1801 ctaaaagatg ggaagatcgt ttaaatgatt atacctatgt cccccgcaca aaagaaatta
     1861 gtgaaaaaaa taaaaaatta gctttacata tagcgagttg gtacgaatct actcgtcaat
     1921 aagtaaccta atctgcaacg attttaaaag ataaataaaa ggcattcttc caacacccat
     1981 ataatttggg tcgttagatg aatgcctaat tttgtgaatt tattttgtag ttcctcggta
     2041 ttcaattctg tgaggtaaaa ctacatttgg ttcttctatc ttttcatcgt tcatatattt
     2101 tgttaataag cgcatcccta ctgcaccgat atcatataat ggttgaataa cactagaaag
     2161 ttgtggtcta accatctcaa ctaatcgtgt attattgaaa ctaataattt gtaattcctc
     2221 tggaacttta ataccagcat ccattgcact atgcataata ccaattgctt cttcgtcgct
     2281 gatacataaa atggcatctg gcaaattgcc tttcattttg gcaaaagctt ttacgccttc
     2341 tttataactt tcagcaccag aacaattcaa tgtatcacct aattgaaggc catttttatt
     2401 taacacttca gttaaacctt ctaaaacatc ttcttgagct tttttagaat gttctccacc
     2461 tactaaagca aatgatttag cgcctttttc aattaattct cccgtaattt ctttcgcagc
     2521 ttcagtaaaa tcaatattaa ctgatgctat atgtgcatcc ttaccatttg ttcctgatac
     2581 tactacaggt acagatgatt gatttatcaa ttctttcatt tcttcagtaa ttgtaccacc
     2641 aaggaaaata ataccatcaa cctgtttact taataagtta ttaaaaattt ctttttcctt
     2701 ttcaggatcg ttatctgaat ttgaaataat tgagtgatat ttatacattg ttgcaatatc
     2761 ttcaagtcca cgagcaagtt gtgaataata gatattagat atatctggaa tgatcacacc
     2821 tactgttgtt gtctttttac tagctaaacc tctagcaaca gcatttggac gataattcaa
     2881 acgcttaatg acttcgttaa ctttattttt agtttctgct ttaacatttt ggttcccatt
     2941 aacaacacgc gacactgtgg ccatagagac acgcgcttct cttgctacat catatatagt
     3001 aactgtcata atttcctcct tgtaaacgtt ttattcatta taataacatg ttttcatttc
     3061 attttcatag tttaccatat tttaatttac agtaacatat ttttgtttgg aaaaatttag
     3121 cgaaaagctt gaaataaaaa caaacgctaa atgataacga acgatatatt ttaattttat
     3181 ataaactttt ttcattttta attgtaatgt tttgagaaag ccttttcaaa tatcaattgc
     3241 atttgttgaa aaatgttcac atttcagcaa caaatgtgtg aatctattat attttacaga
     3301 taattcatag ttataatttt aattactgct atcagtcata atctttaata attgtgaaat
     3361 tgaacaagtg tcgaagtatt ataacaatcg ttaagtgcag tataccttta tttctataca
     3421 aaaaataata gccactctaa aagtaatata ctcttagaat ggctgtaagt ttaaaattgt
     3481 cgattcaaag ataatattct acttcaacat gacatattag ttaagtagtc tatagtttcc
     3541 ttggaatatt attttaactt tttagcgtta tataaatcag ctaaaggctt taattcatca
     3601 taaaatgctt ggaattcatc taaatccatt tgttgacccg catcactaag tgcaacagat
     3661 ggatctggat gcacctcagc cataactcca tcagcaccaa ctgctaatgc tgctttcgca
     3721 gttggtaaca tgatatcttt acgacctgta ctatgcgtta catctaccat gactggtaag
     3781 tgtgtacctt gttttaaaat tggtactgct gaaatatcta aagtgttacg tgtcgccttt
     3841 tcataagttc ggattccacg ttcacataaa ataatgtttt gattaccttg tgaagcaatg
     3901 tattcagctg cataaacaaa ctcttcgatt gtagcagata aaccacgttt taatagaata
     3961 ggctttttcg tacggccagc ttcttttaat aactcgaagt tttgcatatt acgtgcacca
     4021 atttggaata cgtctaaata ctcatcagcc acttcaaaat catttggatt tacgatttcg
     4081 ctgacaacat ttaaatcata tttatcttta atctgtttaa gtattttaag tccttcaaca
     4141 cctaggcctt ggaaatcata tggtgatgta cgtggtttaa atgcaccgcc acgaataaat
     4201 ttttcacctt tagcatgtaa gtttttagca acagcttcaa cttgttcaaa tgattcaact
     4261 gaacatggcc caaatacaaa tgatttattg ccgtctccaa taatgccccc attatcaaat
     4321 gttacaatcg tatcttcagg tttcaactta cgtgatacat ataaatgttt ttcattttca
     4381 gatttttgta aatctgtaga ggctttgaaa atttctttaa ataattgctt aatagtatta
     4441 tcgttgaatg gtcctttgtt actatcgatt aagtcgttaa gcatttcttt ttcacgttgt
     4501 ggatcataga tacgtgtacc ttgttttaat ttttcttccc caattttttg tgctagttca
     4561 ccacgtttag ataataagtc taaaatttga tgattcagtg atacaatctc acttctgtat
     4621 gattctaatt tattactcat ccttactcac ctctactaat tatttcaagt atattccac
//
LOCUS       DAMZKC010000171         4625 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.173, whole genome shotgun sequence.
ACCESSION   DAMZKC010000171 DAMZKC010000000
VERSION     DAMZKC010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4625
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.173"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..837
                     /locus_tag="POB55_002273"
     CDS             <1..837
                     /locus_tag="POB55_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDI6501218.1"
                     /translation="VKNSPQYLEKLINSIDKIANNTFFSSYYSQINDWLNSLPKKIPS
                     MLSEFTDGFGSKIATFAETIANIGVVIVTTPFVLFFMLKDGHHFKEFSTNIMPPKFRK
                     DFHDLLEKMSVQVGSYIQGQIIVSFCIGILLFIGYSVIGLKYSLVLASIAAVTSVVPY
                     LGPTIAISPAIVIAAITSPWMLLKLAVVWTLVQFVEGHFISPNIMGKTLKIHPLTIIF
                     ILLCAGKLLGIVGVILGIPGYAILKVLVTHLFQLFKRRYNRFYGNDVGEYDIKESNKI
                     VE"
     gene            1318..3840
                     /gene="mprF"
                     /locus_tag="POB55_002274"
     CDS             1318..3840
                     /gene="mprF"
                     /locus_tag="POB55_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HDI6501219.1"
                     /translation="MNQEVKNKIFSILKITFATALFIFVVITLYRELSGINFKDTLVE
                     FSKINRMSLVLLFIGGGASLVILSMYDVILSRALKMDISLGKVLRVSYIINALNAIVG
                     FGGFIGAGVRAMVYKNYTHDKKKLVHFISLILISMLTGLSLLSLLIVFHVFDASLILD
                     KITWVRWVLYVVSFFLPLFIIYSMVRPPDKNNRFVGLYCTLVSCVEWLAAAVVLYFCG
                     VIVDAHVSFMSFIAIFIIAALSGLVSFIPGGFGAFDLVVLLGFKTLGVPEEKVLLMLL
                     LYRFAYYFVPVIIALILSSFEFGTSAKKYIEGSKYFIPAKDVTSFLMSYQKDIIAKIP
                     SLSLAILVFFTSMIFFVNNLTIVYDALYDGNHLTYYILLAIHTSACLLLLLNVVGIYK
                     QSRRAIIFAMISILLITVATFFTYASYILITWLAIIFVLLIVAFRRARRLKRPVRMRN
                     IVAMLLFSLFILYVNHIFIAGTLYALDIYTIEMHTSVLRYYFWLTILIIAIIIGMIAW
                     LFDYQFSKVRISSKIEDCEEIINQYGGNYLSHLIYSGDKQFFTNENKTAFLMYRYKAS
                     SLVVLGDPLGDENAFDELLEAFYNYAEYLGYDVIFYQVTDQHMPLYHNFGNQFFKLGE
                     EAIIDLTQFSTSGKKRRGFRATLNKFDELNISFEIIEPPFSTEFINELQHVSDLWLDN
                     RQEMHFSVGQFNEEYLSKAPIGVMRNEENEVIAFCSLMPTYFNDAISVDLIRWLPELD
                     LPLMDGLYLHMLLWSKEQGYTKFNMGMATLSNVGQLHYSYLRERLAGRVFEHFNGLYR
                     FQGLRRYKSKYNPNWEPRFLVYRKDNSLWESLSKVMRVIRHK"
     gene            complement(4043..4552)
                     /gene="msrA"
                     /locus_tag="POB55_002275"
     CDS             complement(4043..4552)
                     /gene="msrA"
                     /locus_tag="POB55_002275"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDI6501220.1"
                     /translation="MNINTAYFAGGCFWCMTKPFDTFDGIEKVTSGYMGGHIENPTYE
                     QVKSGTSGHLETVEIQYDVALFSYNKLLEIFFSVIDPLDTGGQYQDRGPQYQTAIFYT
                     NDHQKELAETYIEQLKNTINADKAIATKILPASQFYKAEDYHQDFYKKNPERYAEEQK
                     IRQEYKNKQ"
BASE COUNT         1436 a          633 c          785 g         1771 t
ORIGIN      
        1 gttaaaaatt caccgcaata tctagaaaaa ttaattaatt ctattgataa aatagcaaat
       61 aatacgtttt tctcttcgta ttatagtcaa attaatgatt ggttaaattc tttacctaag
      121 aaaataccat ctatgttaag tgaatttaca gatggctttg ggtctaaaat tgcaacgttt
      181 gcagaaacga ttgctaatat tggcgttgtg attgtcacaa caccatttgt actattcttt
      241 atgcttaaag atggacatca cttcaaagaa ttttcaacga atattatgcc accgaaattc
      301 cgaaaagatt ttcatgatct acttgaaaaa atgagtgttc aagttggttc atacattcaa
      361 ggacaaatta tcgtttcatt ctgtatcggt atactgttgt ttatcggtta ttcggttatc
      421 gggttgaaat atagcttagt attagctagt attgcggcag ttacaagtgt tgtaccatat
      481 ttagggccta ctatagcgat ttctccagct attgtaatag ctgctataac atcgccgtgg
      541 atgctcttaa aattagcagt agtatggact ttagtacaat ttgttgaagg gcacttcatt
      601 tcaccaaata tcatgggtaa aacacttaag attcatccac ttacaatcat tttcatttta
      661 ctgtgtgcag gcaaattgct tggtattgta ggcgttattt taggtattcc gggatatgct
      721 attttaaaag tattagttac tcatttattc caattattta aacgtcgata caatcgtttc
      781 tatggtaatg atgtaggtga atatgatatt aaagaaagta ataaaatagt tgaataagta
      841 ataaaaaata ccaatgacta tatgtttgtt ggtatttttt atggtgctta taatgttggg
      901 cagttacatt tatgattatc agcacatgtt tagcttataa atttttacga aagtttagcc
      961 gtttataaag cacatgcata atgaaacgag tatttgccac ttgattagta cttcattatt
     1021 atgtcgaaaa taaaaacaag tggtattttt aatatattaa gaagcactca taatcggctg
     1081 ttaattaata atatttttca taagtattga ttcatcattt tctttatgtt aaatatgaat
     1141 cagtatagat aaccatattg ttctgtttga gatagaaata ttttctattt acttcttaat
     1201 acagatatca atatgacaaa agtgttatat aaatcaaagg taaatgatat gctattttta
     1261 tggtttatat gatatatttt gatttataac agaaataatt agaattgatg tgaaaaaatg
     1321 aatcaggaag ttaaaaacaa aatattttca atcttaaaaa ttacgtttgc tacagcttta
     1381 tttatttttg tagtaatcac attgtatcgg gagttatctg gtattaactt taaagatacg
     1441 ttggttgaat ttagtaagat taaccgtatg tccttagttt tactatttat tggtggtggg
     1501 gcatcgcttg ttattctatc aatgtatgat gtgattttat ctagagcttt aaaaatggat
     1561 atatccttag gcaaagtttt aagagtaagt tatatcatca atgcattgaa tgcgattgta
     1621 ggtttcggtg gctttattgg tgcaggcgtt agagctatgg tttataaaaa ctatacgcat
     1681 gataaaaaga aattagttca ctttatatcc ttaatactta tttcaatgtt gacaggttta
     1741 agcttattat cattgttaat tgtattccat gttttcgatg catctttaat tttagataag
     1801 attacatggg taagatgggt attatatgta gtgtcatttt tcttaccatt attcattatt
     1861 tattcaatgg ttagaccacc tgataaaaac aatcgttttg taggattgta ctgcacttta
     1921 gtgtcgtgtg ttgaatggtt agcagctgca gttgtattat atttctgtgg tgtaattgtt
     1981 gacgctcatg tatcattcat gtcctttatt gcaatattta tcattgctgc attatcaggt
     2041 ttagtcagct ttattcctgg tggtttcggc gctttcgatt tagttgtatt actaggattt
     2101 aaaactttag gtgtccctga ggaaaaagta ttattaatgc tacttctata tcgttttgcg
     2161 tactattttg taccggtaat tattgcatta attttatcat catttgaatt tggtacatca
     2221 gctaagaagt acattgaggg atctaaatac tttattcctg ctaaagatgt tacgtcattt
     2281 ttaatgtctt atcaaaagga tattattgct aaaattccat cattatcatt agcaatttta
     2341 gtattcttta caagtatgat cttttttgta aataacttaa cgattgttta cgatgcttta
     2401 tatgatggaa atcacttaac gtattatatt ctattggcaa ttcatactag tgcttgttta
     2461 ttacttttac tgaatgtagt tggtatttat aagcaaagta gacgtgccat catctttgct
     2521 atgatttcaa ttttattaat cacagtggcg acattcttca cttacgcttc atatatttta
     2581 ataacatggt tagctattat ttttgttctg cttattgtag ctttccgtag agcacgtagg
     2641 ttgaaacgcc cagtaagaat gagaaatata gtagcaatgc ttttattcag tttatttatt
     2701 ttatatgtta accatatatt tattgctgga acgttatatg cattagatat ttatacgatt
     2761 gaaatgcata catctgtatt gcgctattac ttctggctta cgattttaat catcgctatc
     2821 atcataggta tgattgcatg gttgtttgat tatcaattta gcaaagtacg catttcttct
     2881 aaaattgaag attgcgagga gattattaat cagtatggcg gtaattattt gagtcacttg
     2941 atatatagtg gtgacaagca gtttttcact aatgaaaata aaacagcatt tttaatgtat
     3001 cgttataaag caagttcatt agtggttctt ggagatccgt taggtgatga aaatgccttt
     3061 gatgaattgt tagaagcatt ctataattac gctgagtatt taggctatga tgttatattc
     3121 tatcaagtta cagatcaaca catgccttta tatcataatt tcggtaacca atttttcaaa
     3181 ttaggtgaag aagcaattat tgatttaacg caattttcaa cttcaggtaa aaaacgccgt
     3241 ggatttagag cgactttaaa caaattcgat gaacttaata tttcattcga aattattgaa
     3301 ccaccgtttt caactgaatt tataaatgaa cttcaacatg taagtgattt atggctagat
     3361 aatcgtcagg aaatgcattt ctctgtgggt caatttaatg aagaatactt atctaaagcg
     3421 ccaattggtg taatgcgaaa tgaagaaaat gaagtaattg cattttgtag tttaatgcca
     3481 acatacttta atgatgccat ttcagtcgat ttaattagat ggttgccaga gttagattta
     3541 ccattaatgg atggtctata cttgcatatg ttactttgga gtaaagaaca aggttataca
     3601 aaatttaata tgggtatggc aacgttatcg aacgttggtc aattgcatta ttcatattta
     3661 agagaacgac ttgcaggccg tgtctttgaa catttcaacg gtctatatcg tttccaagga
     3721 ttacgtcgtt ataaatctaa atataatccg aattgggaac cacgcttttt agtttatcgt
     3781 aaagataatt cgctttggga atcactttct aaagtaatgc gtgtaatacg tcacaaataa
     3841 ttaaaatcca agtgctaaga ggtatacagt tatgcctgaa gcgcttggat tttttggttt
     3901 ctataaagaa tgatttataa aaaacggtac agataagaat atttaagttt cctatctgta
     3961 ccgtatttta atattgaaat gagcttaagt taagtcgcct aacttttatt atcagtccaa
     4021 tcagtttcat caagtataaa tgttattgct tatttttgta ttcttggcgt attttttgtt
     4081 cttctgcata gcgctctgga tttttcttat aaaaatcttg gtgatagtct tcggctttgt
     4141 aaaattgtga cgcgggtaat atttttgttg caattgcctt atcagcatta atcgtatttt
     4201 taagctgctc gatataagtc tcagcgagtt ctttttgatg atcattagtg tagaaaatag
     4261 ctgtttgata ttgaggacca cggtcttgat attgaccccc tgtatctaat gggtcaatga
     4321 ctgagaaaaa tatttctaat aacttattgt atgagaataa tgcaacatca tattgaattt
     4381 caacagtttc taaatgacca ctcgtacctg attttacttg ttcgtaagta ggattttcaa
     4441 tatgtccgcc catatatcca gaagttactt tttctatgcc gtcaaaggtg tcaaatggtt
     4501 tcgtcataca ccaaaagcaa cctccggcaa aataagctgt attaatattc atttttgaca
     4561 tcctttcatt agaccttagt acgatttatt aagaaatcac ttgctttttg aattgttttt
     4621 atata
//
LOCUS       DAMZKC010000172         4610 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.174, whole genome shotgun sequence.
ACCESSION   DAMZKC010000172 DAMZKC010000000
VERSION     DAMZKC010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4610
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.174"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            13..453
                     /locus_tag="POB55_002276"
     CDS             13..453
                     /locus_tag="POB55_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDI6501221.1"
                     /translation="MSEIKRLEINYKTDELFENFRAFGNKDLYMVNELNGQMIDASSD
                     SPFYGIFVGDQLGARMALLKKGDVEEIYFPDFEDYILLWKLEVLPKYQNRGYASELID
                     FAKSFNMPIKAIGRNDSKDFFLHHGFTDVEAKNIEGHDVLLWKP"
     gene            complement(562..1257)
                     /locus_tag="POB55_002277"
     CDS             complement(562..1257)
                     /locus_tag="POB55_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HDI6501222.1"
                     /translation="MGKLGYKNILIDFDDTIVDFYDAEEWAFHYMANVFNHKATKDDF
                     LTFKKINHQHWEAFQQNKLTKSEVLSERFVNYFKHHQMEVDGHRADVLFRNGLAEAKV
                     KYFDQTLETIVELSKRHDLYIVTNGVTETQKRRLNQTPLHKYIKKIFISEETGYQKPN
                     PDFFNYVFNDIGEDERQHSIIVGDSLTSDILGGINAGIATCWFNFRGFDHNPGIIPDY
                     EINSWKQLNDIVR"
     gene            complement(1381..1515)
                     /locus_tag="POB55_002278"
     CDS             complement(1381..1515)
                     /locus_tag="POB55_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class phenol-soluble modulin"
                     /protein_id="HDI6501223.1"
                     /translation="MTGLAEAIANTVQAAQQHDSVKLGTSIVDIVANGVGLLGKLFGF
                     "
     gene            complement(1572..1706)
                     /locus_tag="POB55_002279"
     CDS             complement(1572..1706)
                     /locus_tag="POB55_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class phenol-soluble modulin"
                     /protein_id="HDI6501224.1"
                     /translation="MEGLFNAIKDTVTAAINNDGAKLGTSIVSIVENGVGLLGKLFGF
                     "
     gene            2070..2143
                     /locus_tag="POB55_002280"
     tRNA            2070..2143
                     /locus_tag="POB55_002280"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2104..2106,aa:Arg,seq:tct)
     gene            complement(2618..2806)
                     /locus_tag="POB55_002281"
     CDS             complement(2618..2806)
                     /locus_tag="POB55_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDI6501225.1"
                     /translation="MRNQIQKLLDSDLSSLHISKQTGVPQSTIHRMRKNERSLDNMSL
                     KNAELLYKFANSIFSNEN"
     gene            3055..4002
                     /locus_tag="POB55_002282"
     CDS             3055..4002
                     /locus_tag="POB55_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HDI6501226.1"
                     /translation="MRLQKAPLVTSGLVLGLLGLGNLLKDLSLTLNAVCGIFAFLIWI
                     HLLCTMIKYFNNVKEQLNSPLVSSVFTTFFMSGFLGTTYLNTFFSNITFINSLITPIW
                     ILCLVGIMTHMIIFSIKYLKDFSLENVYPSWTVLFIGIAIAGLTAPVSGYFFIGQLTV
                     IYGFVATSIVLPIVFKRLKAFPLQTSIKPNTSTICAPFSLVAAAYVIAFPKANAFIVI
                     IFLILAQIFYFYIIIQLPKLLKEPFSPVFSAFTFPLVISATALKNSLPVLMFPDIWKG
                     LLFIEVLLATVIVLRVFIGYLHFFLKKEKQDKFLRNASQ"
     gene            complement(4308..4535)
                     /locus_tag="POB55_002283"
     CDS             complement(4308..4535)
                     /locus_tag="POB55_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501227.1"
                     /translation="MTHLTKVLDTLTGICVVLLFSKYFVAYANMVFDWNLRWYLLENI
                     PHLPIILFILMFIFGVPSEMIKDRQRKNNGV"
BASE COUNT         1514 a          789 c          621 g         1686 t
ORIGIN      
        1 gggtagataa atatgagtga aatcaaacgt cttgaaatta attacaaaac tgacgaatta
       61 ttcgaaaact ttagagcgtt tggcaacaaa gacttataca tggtcaatga gttaaacggt
      121 caaatgattg atgcaagttc agattcacca ttttatggca tatttgtcgg agatcaatta
      181 ggagctagaa tggcattact aaaaaaaggt gatgtcgaag aaatctactt cccagatttt
      241 gaagattata tattattatg gaagttagaa gtattaccaa aatatcaaaa cagagggtac
      301 gcttcagaat tgattgattt tgcaaagagt ttcaatatgc caattaaagc cattggcaga
      361 aatgattcta aggatttctt tttacatcat ggatttacag atgtggaagc taaaaatata
      421 gagggacatg atgtcttatt gtggaaacca taagataata atattcgaca ctacgagcat
      481 gaaaatgcat cttttcgtag tgtctttttt acaattactt tcttaagcta atataagtaa
      541 atcattttca aattatttgt cttaacgtac aatatcattt agttgtttcc atgaattaat
      601 ttcataatca ggtataattc ctggattatg atcaaatcct ctaaaattaa accagcaagt
      661 agctataccc gcattgattc cacctagaat gtcagatgtt aaagaatctc caactataat
      721 cgagtgctgt ctttcatcct caccaatatc attaaaaaca taattaaaaa aatccggatt
      781 aggtttttga tatcctgttt cttcagatat aaatatcttt ttaatatatt tatgcaacgg
      841 cgtctgattt aaccttctct tttgcgtttc ggttacacca ttagtaacaa tatataaatc
      901 atgtcttttc gataattcga caattgtttc taatgtttga tcaaagtatt taactttagc
      961 ttctgctaat ccatttctaa ataacacatc tgcacgatgc ccatcaactt ccatttgatg
     1021 atgtttgaag taattcacaa atcgttctga taatacttca gacttcgtta atttattttg
     1081 ttgaaaagct tcccaatgtt ggtgattgat ttttttaaat gttaaaaaat catcctttgt
     1141 tgctttatga ttaaaaacat tcgccatata gtgaaacgcc cattcttctg catcataaaa
     1201 atcaacaatt gtatcatcaa agtctatcaa aatattttta tatcccaatt tccccatctc
     1261 ctatattgtc tatgtatcta aatcttaaca gaggctcaaa tttctgcaaa taaaataaac
     1321 tgagtgcata acattaaagt atgctcaccc agtttatttt aaagaatatt agttattata
     1381 ttagaatcca aataatttac ctagtaaacc cacaccgtta gcaacgatgt ctacgatact
     1441 tgtgcctaat ttcacactat catgttgttg tgcagcttgc acagtatttg cgattgcttc
     1501 tgctagtcca gtcatttaaa tctctccctc acctttgaaa taatactgat tacttacata
     1561 acatattgaa attagaatcc gaataattta cctaataaac ctacgccatt ttcaacgatg
     1621 ctcacaatgc ttgtgcctaa ttttgcgcca tcattattaa ttgctgcagt tacggtatct
     1681 ttaattgcgt taaataaacc ttccattgaa aacactcctt aaaatttaaa tttgaagata
     1741 acaaaaacgt gcgtagcttt taaatcaccg aaatgttatt cgcttaacgt tttgttgttg
     1801 ttattttaaa ataaatttga tgcaattagt ttgtttatcc gcacaacatc ttataatgta
     1861 cttaactgta ttttaaagag aaaagaaata cagttaggca ttcaaaactg tatttaacac
     1921 aattaagttg cctgaattcg tatttaagtc ttattgaacc tttttagata aatagctcta
     1981 taatagtgaa aaatataaac attttttatt tacaaggtat tgctaattta agttcattta
     2041 gatataataa ttcttgtgtt gttaaacgtg tcctggtagc tcagctggat agagcaatgg
     2101 ccttctaagc catcggtcgg gggttcgaat ccctcccagg acgtttatag gtatttttat
     2161 acgcattacc aaacaaaaga gttccgtgat tacggggctc tttttgtttt gaatttcagt
     2221 aatatagtat gatgcgtcac caaaacgtcc cccgcataag ccccgaaaat acagtaatta
     2281 aaacaagcat gcttattcgt tatagaattt tttgacacac aattgacacg cgtctgacac
     2341 ttgtttatac atttttaatt aagtaatttt gtgctcaaat ttcatctata ctgcacctga
     2401 actacaccaa cactacacca agatttttaa cactcaccat ttgcatgcgt agagattttt
     2461 attattatat tattcctata gattttgata ctattcaaaa ttttagggac ttttcagggg
     2521 cccgaaatcc tataattata attatataca tctaaaaaaa ataaccacgt ccatcgagac
     2581 gtggtttttt aattatttaa ctcactaatt attcttctta attttcattg ctaaatatac
     2641 tattggcaaa tttataaagt agttcagcgt ttttcaatga catattgtct aatgatcttt
     2701 cattttttct cattctgtgt attgtgcttt gtggaactcc tgtttgtttc gatatatgta
     2761 aactgctcaa atcactgtct aatagttttt gaatttgatt tctcattttg tacaccccct
     2821 tgtaaatctt caatcatcat ttaaattaaa taattacttt tccacatatt ccaatattta
     2881 ggttgcaaag catacctcaa atatcattaa ttttgagatt taaatgtcaa ttatgtgtct
     2941 atcaatccaa tatacatact ctaataacgt aatagtacac actcttctta ttaattggcc
     3001 atagctatca tgatataatt agtgaagaaa atcacataag aaaggttgta aatcatgaga
     3061 cttcaaaaag cacctctagt aacgtcagga ctagtcttag gattattagg cctgggtaat
     3121 ctattaaaag acttatctct tactttaaac gctgtttgcg gaatctttgc tttcttgatt
     3181 tggattcacc ttttatgtac tatgatcaaa tattttaata atgtgaaaga acaattaaac
     3241 agtcctctag tttcatcagt gttcacaaca tttttcatgt ctggcttttt aggtactact
     3301 tatttaaata cattttttag taacataact tttatcaata gcttaataac gcctatttgg
     3361 attttatgcc ttgtgggaat tatgacgcat atgattattt tttcaataaa atatttaaaa
     3421 gatttttcac ttgaaaatgt ttatccttcg tggactgtac tttttattgg tattgctatc
     3481 gcaggattga cggcacccgt tagcggatat tttttcatag gtcaattaac agtaatatat
     3541 ggctttgtag ctacttctat tgtcttacct atagttttca agcgattaaa agcatttcca
     3601 ttgcagacgt caatcaaacc gaacacatcg acaatttgtg caccattttc tttagtcgct
     3661 gcagcatatg ttatagcctt tcctaaggcg aatgctttta tcgtaattat atttttaata
     3721 ttagctcaaa tattttattt ttatatcatt atacaattgc ctaaattact aaaagaacct
     3781 ttttcgcccg tattttcagc tttcacattc cctttagtaa tctcagcaac tgctttaaag
     3841 aacagtttgc ctgtacttat gtttccagac atttggaaag gtcttttgtt tatcgaagtg
     3901 ttattagcca ctgtaatagt acttagagtc tttataggat accttcactt ctttttaaaa
     3961 aaggaaaaac aagataaatt tcttcgtaat gcgtctcagt aacactatta ccaagaatta
     4021 acacgcatat ttaataacta aaaaagaggc aggtaatctt gcctcttttt agtgtatatc
     4081 aatgcctctt tgatttaata tgatcttatc aatgatacct tctgactaaa gctgtagtaa
     4141 ctatactaga acgtctagtt cataaacacg tttcactcta acctttattt aaataatata
     4201 taagcagcaa acgccaaggc taatatacca ataaaataat taataaagat ttttatttta
     4261 gtctttgtaa tagttgacaa attttatcac ctctaaatat cgataaatta aacgccgtta
     4321 tttttccttt gcctatcttt tatcatttca gaaggtactc cgaaaataaa catcagaata
     4381 aataatataa ttggcaaatg tggtatgttt tctagcaaat accatcttaa attccaatca
     4441 aacaccatat ttgcatacgc cacaaaatat ttactaaata ataatactac gcatattcca
     4501 gttagtgtat ctaaaacctt tgtcaaatgt gtcatttata gccatccttt atatatgcta
     4561 ttttcacaac catatgtatc atgtgttaca aaaactgttt acagcaaata
//
LOCUS       DAMZKC010000173         4603 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.175, whole genome shotgun sequence.
ACCESSION   DAMZKC010000173 DAMZKC010000000
VERSION     DAMZKC010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4603
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.175"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            226..1401
                     /gene="rpsA"
                     /locus_tag="POB55_002284"
     CDS             226..1401
                     /gene="rpsA"
                     /locus_tag="POB55_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HDI6501228.1"
                     /translation="MTEEFNESMINDIKEGDKVTGEVQQVEDKQVVVHINGGKFNGII
                     PISQLSTHHIDSPSEVVKEGDEVEAYVTKVEFDEENETGAYILSRRQLETEKSYSYLQ
                     EKLDNNEIIEAKVTEVVKGGLVVDVGQRGFVPASLISTDFIEDFSVFDGQTIRIKVEE
                     LDPENNRVILSRKAVEQEENDAKKDQLLQSLNEGDVIDGKVARLTQFGAFIDIGGVDG
                     LVHVSELSHEHVQTPEEVVSIGQDVKVKIKSIDRDTERISLSIKDTLPTPFENIKGQF
                     HENDVIEGVVVRLANFGAFVEIAPGVQGLVHISEIAHKHIGTPGEVLEPGQQVNVKIL
                     GIDEENERVSLSIKATLPNEDVVESDPSTTKAYLENEEEDNPTIGDMIGDKLKNLKL"
     gene            1623..2933
                     /gene="der"
                     /locus_tag="POB55_002285"
     CDS             1623..2933
                     /gene="der"
                     /locus_tag="POB55_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019167598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HDI6501229.1"
                     /translation="MTKPIVAIVGRPNVGKSTIFNRIVGERVSIVEDTPGVTRDRIYS
                     SGEWLTHDFNIIDTGGIEIGDAPFQTQIRAQAEIAIDEADVIIFMVNVREGLTQSDEM
                     VAQILYKSKKPVVLAVNKVDNMEMRTDVYDFYSLGFGEPYPISGSHGLGLGDLLDAVV
                     SHFGEEEEDPYDEDTIRLSIIGRPNVGKSSLVNAILGEDRVIVSNVAGTTRDAIDTEY
                     SYDGQDYVLIDTAGMRKKGKVYESTEKYSVLRALKAIERSNVVLVVIDAEQGIIEQDK
                     RVAGYAHEQGKAVVIVVNKWDTVEKDSKTMKKFEDEVRKEFQFLDYAQIAFVSAKERT
                     RLRTLFPYINEASENHKKRVQSSTLNEVVTDAISMNPTPTDKGRRLNVFYATQVAIEP
                     PTFVVFVNDVELMHFSYKRYLENQIRAAFGFEGTPIHIIARKRN"
     gene            2950..3948
                     /locus_tag="POB55_002286"
     CDS             2950..3948
                     /locus_tag="POB55_002286"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDI6501230.1"
                     /translation="MTKITVFGMGSFGTALANVLAENGHDVLMWGKNQDAVDELNTCH
                     TNKKYLKYAKLDVNIIATSDMTKAIQFADIYLMALPTKAMREVASQINDKLTSKKTFI
                     HVAKGIENGTFKRVSEMIEDSISPEYNAGIGVLSGPSHAEEVVVKQPTTVAASSKDKS
                     VSKLTQDLFMNDYLRVYTNDDLIGVELGGALKNIIAVASGIVAGIGYGDNAKAALMTR
                     GLAEISRLGEKLGADPMTFLGLGGIGDLIVTCTSTHSRNFTLGYKLGQGESMDQALSE
                     MNMVVEGIYTTKSVYHLAKEKNVDMPITNALYRVLFENISVKECVKDLMERDKKSE"
     gene            4119..4391
                     /locus_tag="POB55_002287"
     CDS             4119..4391
                     /locus_tag="POB55_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HDI6501231.1"
                     /translation="MNKTDLINAVAEQADLTKKEAGSAVDAVFESIQNSLAKGEKVQL
                     IGFGNFEVRERAARKGRNPQTGKEIDIPASKVPAFKAGKALKDAVK"
BASE COUNT         1660 a          613 c          911 g         1419 t
ORIGIN      
        1 tatgctaatt tttatacaaa aaatatgtat aaacatagca aactgtatta ctttgataca
       61 aaaatggttg taataaatat ttatcgatat gacgacttga atatgataaa gtgacatatt
      121 tatgtatatg actatttcgc aaaatgtaat cgaggtagaa tttcttgaca attctgtcag
      181 tttataagat gttataaata tgtagtgtat aaggaggcaa acaagatgac tgaagaattc
      241 aatgaatcaa tgattaacga tattaaagaa ggtgacaaag tcactggcga ggtacaacaa
      301 gttgaagaca agcaagttgt tgttcatatc aacggtggta aatttaatgg gattattcct
      361 attagtcaac tatctacgca tcatattgat agcccaagtg aagttgtaaa agagggcgac
      421 gaagttgaag catatgtcac taaagttgag tttgatgaag aaaatgaaac tggagcttac
      481 atcttatcta gaagacaact tgaaactgag aagtcttata gttatttaca agaaaaatta
      541 gataataatg aaatcatcga agcgaaagta acagaagtag ttaaaggtgg tttggttgtt
      601 gatgtaggac aaagaggttt tgttccggct tcactaattt caacagactt cattgaggat
      661 ttctctgtgt ttgatggaca aacaattcgt attaaagttg aagaattgga tcctgaaaat
      721 aatagagtca ttttaagccg taaagcagtt gaacaagaag aaaacgatgc taaaaaagat
      781 caattattac aatctttaaa tgaaggcgat gttattgatg gtaaagtagc gcgtttaact
      841 caatttggtg catttataga cattggcggt gttgatggtt tagtgcatgt atctgaactt
      901 tctcacgaac atgttcaaac accagaagaa gtagtttcaa ttggtcaaga tgttaaagtt
      961 aaaattaaat ctattgatag agatacagaa cgtatttcat tatcaatcaa agatacgtta
     1021 ccaacacctt tcgaaaatat taaaggtcaa ttccacgaaa atgatgtcat tgaaggtgtc
     1081 gtagtaagat tggcaaactt tggtgcattt gttgaaattg caccaggtgt acaaggactt
     1141 gtacatattt ctgaaattgc acacaaacac attggtacgc caggtgaagt gttagaacct
     1201 ggtcaacaag taaatgttaa aatattaggt attgatgaag agaatgaaag agtatcacta
     1261 tctattaaag caacattacc aaacgaagat gttgttgaaa gtgatccttc tacgactaag
     1321 gcgtacttag aaaacgaaga agaagataat ccaacaattg gcgatatgat tggtgataaa
     1381 cttaaaaatc ttaaactata atttaatatt taatagtcaa ctccacatgt ttatgattgc
     1441 atgtggagta tttttatgta acaaaatata ctcggaatga taacgtggac aaatttaact
     1501 aagtgtttaa aaagataaag ttttaagtgc tgatttttat cattacagta ataaactcat
     1561 tttgaataca cagtctcatg tgatattatt aaaaagatat aagaaagaga ggaagttagc
     1621 ttatgactaa acctatagta gctattgtag gtaggcctaa tgtaggtaaa tctacaattt
     1681 ttaatagaat agttggagaa cgtgtttcga ttgtggaaga cacgccaggt gtaacacgag
     1741 atcgtattta ttcttcaggt gaatggttaa cacatgattt caatattatt gatacaggtg
     1801 gtattgaaat tggtgatgca ccattccaaa cacaaattag agcgcaggca gaaatcgcca
     1861 tagatgaagc ggatgttatt atttttatgg ttaacgtgcg tgaaggattg acacaaagcg
     1921 atgaaatggt cgctcaaatt ttatacaaat ctaaaaaacc ggtcgtatta gcggttaaca
     1981 aagtagataa tatggaaatg cgtacagacg tgtatgattt ctattcatta ggatttggtg
     2041 aaccgtatcc gatatcaggg tcacatggtt taggtcttgg tgacttgtta gatgcagttg
     2101 tttctcattt tggtgaagag gaagaagatc cttatgatga agatacaatt cgactatcca
     2161 ttattggacg accaaacgta ggtaaatcaa gtttagtaaa tgctatttta ggtgaagatc
     2221 gcgttatcgt ttctaatgtt gcagggacaa cgagagacgc tattgataca gagtatagtt
     2281 atgatggaca agattatgtt ttaatcgata ctgctggtat gcgtaaaaaa ggaaaagtat
     2341 atgaatcaac tgagaaatat tcagtattaa gagctttaaa agcgattgaa cgttcaaatg
     2401 ttgttttagt ggtcatagat gcagaacaag gcatcattga acaagataaa cgtgttgcag
     2461 gatatgcaca tgaacaaggt aaagcagtcg tgattgtcgt aaataaatgg gatactgtgg
     2521 aaaaagatag taaaacgatg aagaaatttg aagatgaagt acgtaaagaa ttccaatttt
     2581 tagattatgc acaaattgct tttgtgtctg ctaaagaacg cacaagatta cgtacattat
     2641 tcccttacat caatgaagca agtgaaaacc ataaaaaacg tgttcaaagt tcaactttaa
     2701 atgaagttgt tactgatgca atttccatga accctacacc aacagacaaa ggtagacgtt
     2761 tgaatgtctt ttatgcaaca caagttgcta tagaaccacc gacatttgtt gtatttgtta
     2821 atgatgtaga attaatgcat ttttcttata aacgctattt agagaatcaa atccgtgccg
     2881 cttttggttt tgaaggtaca ccaattcata ttatagctcg aaagagaaat taacgattgg
     2941 gggataacaa tgactaaaat taccgttttt ggtatgggaa gttttgggac agcccttgcc
     3001 aatgttcttg cagaaaatgg acatgatgtt ttgatgtggg gtaaaaatca agatgctgtt
     3061 gatgaattaa atacatgtca tacaaataaa aagtatttaa aatacgcgaa attagatgtt
     3121 aacatcatcg ctacttcaga tatgaccaaa gcaattcaat ttgcagatat ttacttaatg
     3181 gctttaccta ctaaagcaat gcgagaagtt gcttctcaaa ttaatgataa gctgacctct
     3241 aaaaagactt ttatacatgt tgctaaaggt attgaaaatg ggacgtttaa acgtgtgtca
     3301 gaaatgattg aagattctat ttcacctgaa tataatgcag gtattggcgt gttgtcaggg
     3361 ccaagtcatg cggaagaagt tgtagtcaag caaccaacta cagttgctgc ttcatcaaaa
     3421 gataaaagtg taagtaaatt aacgcaagat ttatttatga atgattattt gcgtgtgtac
     3481 acgaatgatg acttgattgg tgttgaactt ggtggtgcat tgaaaaatat catcgcagta
     3541 gcaagtggta tcgtagctgg aattggctac ggtgataatg caaaagctgc attaatgact
     3601 cgtggtttag cggaaattag tagattaggt gaaaagttag gtgccgatcc tatgacattt
     3661 ctaggtttag gtggtatcgg tgacttaatc gttacttgca catcaacaca ttctcgaaat
     3721 ttcacattag gatataaact tggacaaggt gaatcaatgg atcaagcatt atctgaaatg
     3781 aatatggtcg ttgaaggtat ttatacaact aaatcagttt atcatttagc taaagaaaaa
     3841 aatgtggata tgccaattac aaatgcatta tatagagtat tatttgaaaa tatctcagta
     3901 aaagaatgcg taaaagattt aatggagcgc gataaaaaat ctgaataaaa tgtgtattat
     3961 tacacatata cggctaataa ttacgtaaaa tgaatagaaa tggcttaaat tcaacgtttt
     4021 tgccacataa ataattgcga tttatgtaat gcttgtgtta aagttttacc ataatgatat
     4081 aaacatatca ttatcaaacc tcaggaggtg aatgtctaat gaacaaaaca gatttaatca
     4141 atgcagttgc agagcaagct gatttaacta aaaaagaagc tggttcagca gtagatgctg
     4201 tattcgaatc aatccaaaac tcacttgcta aaggtgaaaa agtacaatta attggtttcg
     4261 gtaactttga ggtacgtgaa cgtgctgcac gtaaaggtcg taaccctcaa actggtaaag
     4321 aaattgatat cccagcaagt aaagttccag cattcaaagc tggtaaagca ttaaaagatg
     4381 ctgtaaaata attttactta aaaagccctg aataagggct ttttattttg cttttaatac
     4441 ttacaacctg tacataaatt gtaatgttct tctaagtttt taatctttgg aacattcatt
     4501 gtttaagcgt tcaaaatttt acatgacata atagttttga taaaaatagc aactacaaaa
     4561 tatacatatg aataatgaga ttaattgtta gcgttgaatt tac
//
LOCUS       DAMZKC010000174         4600 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.176, whole genome shotgun sequence.
ACCESSION   DAMZKC010000174 DAMZKC010000000
VERSION     DAMZKC010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4600
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.176"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..557
                     /locus_tag="POB55_002288"
     CDS             <1..557
                     /locus_tag="POB55_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDI6501232.1"
                     /translation="DSDSNSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSASDS
                     DSDSDSDSDSDSDSDSDSDSDSDSESDSDSDSDSDSDSDSDSDSASDSDSGSDSDSSS
                     DSDSESDSNSDSESGSNNNVVPPNSPKNGTNASNKNEAKDSKEPLPDTGSEDEANTSL
                     IWGLLASIGSLLLFRRKKENKDKK"
     gene            778..2304
                     /gene="vwb"
                     /locus_tag="POB55_002289"
     CDS             778..2304
                     /gene="vwb"
                     /locus_tag="POB55_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000791729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor binding protein Vwb"
                     /protein_id="HDI6501233.1"
                     /translation="MKNKLLVLSLGALCVSQIWESNRASAVVSGEKNPYVSESLKLTN
                     NKNKSRTVEEYKKSLDDLIWSFPNLDNERFDNPEYKEAMKKYQQRFMAEDEALKKFFS
                     EEKKIKNGNTDNLDYLGLSHERYESVFNTLKKQSEEFLKEIEDIKKDNPELKDFNEEE
                     QLKCDLELNKLENQILMLGKTFYQNYRDDVESLYSKLDLIMGYKDEERANKKAVNKRM
                     LENKKEDLETIIDEFFSDIDKTRPNNIPVLEDEKQEEKNHKNMAQLKSDTEAAKSDES
                     KRSKRSKRSLNTQNHKPASQEVSEQQKAEYDKRAEERKARFLDNQKIKKTPVVSLEYD
                     FEHKQRIDNENDKKLVVSAPTKKPTSPTTYTETTTQVPMPTVERQTQQQIIYNAPKQL
                     AGLNGESHDFTTTHQSPTTSNHTHNNVVEFEETSALPGRKSGSLVGISQIDSSHLTER
                     EKRVIKREHVREAQKLVDNYKDTHSYKDRLNAQQKVNTLSEGHQKRFNKQINKVYNGK
                     "
     gene            2655..3677
                     /gene="emp"
                     /locus_tag="POB55_002290"
     CDS             2655..3677
                     /gene="emp"
                     /locus_tag="POB55_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular matrix protein-binding adhesin
                     Emp"
                     /protein_id="HDI6501234.1"
                     /translation="MKKKLLVLTMSTLFATQIMNSNHAKASVTESVDKKFVVPESGIN
                     KIIPTYNEFKKAPKVNVGNLADNKNFVASEDKLNKIVDSSAASKIVDKNFAVPESKLG
                     NIVPEYKEINNRVNVATNNPASQQVDKHFVAKGPEVNRFITQNKVNHHFITTQTHYKK
                     VITSYKSTHVHKHVNHAKDSINKHFIVKPSESPRYTHPSQSLIIKHHFAVPGYHAHKF
                     VTPGHASIKINHFCVVPQINSFKVIPPYGHNSHRMHVPSFQNNTTATHQNAKVNKAYD
                     YKYFYSYKVVKGVKKYFSFSQSNGYKIGKPSLNIKNVNYQYAVPSYSPTHYVPEFKGS
                     LPAPRV"
     gene            4012..4533
                     /locus_tag="POB55_002291"
     CDS             4012..4533
                     /locus_tag="POB55_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001627793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501235.1"
                     /translation="MKRKVLVLTMGVICATQLWHSNHANALVSESVETNFVVKDSDNK
                     NILQTHTEITTEEKFSVVEKSQLNTLKSLSNDNYIEYDLHTNQTGIKKGWLYGYSEID
                     SSHFTDRDKRVIRRDHVKEAQSLINDYKYTQKYEDLAKATAKVSTLSQSHQNYLNKQI
                     DKVNNKIEKTEKR"
BASE COUNT         1888 a          651 c          752 g         1309 t
ORIGIN      
        1 gcgattcaga ttccaacagt gactcagatt ccgacagtga ctcggattca gatagcgatt
       61 cagattccga cagtgactca gattccgaca gtgactcaga ctcagacagt gattcggatt
      121 cagcgagtga ttcggattca gatagcgact cagactcgga tagcgactcg gattcagata
      181 gcgattcgga ctcagatagc gattcagaat cagacagcga ttcagattca gacagcgact
      241 cagattcaga tagtgactcg gattcagcga gtgattcaga ctcaggtagt gactccgatt
      301 catcaagtga ttccgactca gaaagtgatt caaatagcga ttccgagtca ggttctaaca
      361 ataatgtagt tccgcctaat tcacctaaaa atggtactaa tgcttctaat aaaaatgagg
      421 ctaaagatag taaagaacca ttaccagata caggttctga agatgaagca aatacgtcac
      481 taatttgggg attattagca tcaataggtt cattactact tttcagaaga aaaaaagaaa
      541 ataaagataa gaaataagta ataatgatat taaattaatc atatgattca tgaagaagcc
      601 accttaaaag gtggcttttt tacttggatt ttccaaatat attgtttgaa tataattaat
      661 aattaattca tcaacagtta attattttaa aaaggtagat gttatataat ttggcttggc
      721 gaaaaaatag ggtgtaaggt aggttgttaa ttagggaaaa ttaaggagaa aatacagttg
      781 aaaaataaat tgctagtttt atcattggga gcattatgtg tatcacaaat ttgggaaagt
      841 aatcgtgcga gtgcagtggt ttctggggag aagaatccat atgtatctga gtcgttgaaa
      901 ctgactaata ataaaaataa atctagaaca gtagaagagt ataagaaaag cttggatgat
      961 ttaatatggt cctttccaaa cttagataat gaaagatttg ataatcctga atataaagaa
     1021 gctatgaaaa aatatcaaca gagatttatg gctgaagatg aggctttgaa gaaatttttt
     1081 agtgaagaga aaaaaataaa aaatggaaat actgataatt tagattatct aggattatct
     1141 catgaaagat atgaaagtgt atttaatact ttgaaaaaac aaagtgagga gttcttaaaa
     1201 gaaattgaag atataaaaaa agataaccct gaattgaaag actttaatga agaggagcaa
     1261 ttaaagtgcg acttagaatt aaacaaatta gaaaatcaga tattaatgtt aggtaaaaca
     1321 ttttatcaaa actatagaga tgatgttgaa agtttatata gtaagttaga tttaattatg
     1381 ggatataaag atgaagaaag agcaaataaa aaagcagtta acaaaaggat gttagaaaat
     1441 aaaaaagaag acttagaaac cataattgat gaatttttta gtgatataga taaaacaaga
     1501 cctaataata ttcctgtttt agaagatgaa aaacaagaag agaaaaatca taaaaatatg
     1561 gctcaattaa aatctgacac tgaagcagca aaaagtgatg aatcaaaaag aagcaagaga
     1621 agtaaaagaa gtttaaatac tcaaaatcac aaacctgcat ctcaagaagt ttctgaacaa
     1681 caaaaagctg aatatgataa aagagcagaa gaaagaaaag cgagattttt ggataatcaa
     1741 aaaattaaga aaacacctgt agtgtcatta gaatatgatt ttgagcataa acaacgtatt
     1801 gacaacgaaa acgacaagaa acttgtggtt tctgcaccaa caaagaaacc aacatcaccg
     1861 actacatata ctgaaacaac gacacaggta ccaatgccta cagttgagcg tcaaactcag
     1921 caacaaatta tttataatgc accaaaacaa ttggctggat taaatggtga aagtcatgat
     1981 ttcacaacaa cgcatcaatc accaacaact tcaaatcaca cgcataataa tgttgttgaa
     2041 tttgaagaaa cgtctgcttt acctggtaga aaatcaggat cactggttgg tataagtcaa
     2101 attgattctt ctcatctaac tgaacgtgag aagcgtgtaa ttaagcgtga acacgttaga
     2161 gaagctcaaa agttagttga taattataaa gatacacata gttataaaga ccgattaaat
     2221 gcacaacaaa aagtaaatac tttaagtgaa ggtcatcaaa aacgttttaa taaacaaatc
     2281 aataaagtat ataatggcaa ataattaatg catggctgca aagcaaataa tgagtttgtc
     2341 gtaaaaataa caacatttta aactagcaat aaataatatc aaagtcatca tttcaatgat
     2401 gcaatctagt atagtccaca ttctaaacag gtgtggacta ttactttttt cactttatat
     2461 tacgaaaaaa ttattatgct taactatcaa tatcaataat taattttaag ctgaaaaaca
     2521 ataaaaatgt taagacaacg tttacttcaa gttaattatt atactgaaaa ttctggtata
     2581 taatgctgtt agtgaatata acagggaaat tatattggtt ataatattga gtctatataa
     2641 aggagaaata acagatgaaa aagaaattat tagttttaac tatgagcacg ctatttgcta
     2701 cacaaattat gaattcaaat cacgctaaag catcagtgac agagagtgtt gacaaaaaat
     2761 ttgtagttcc agaatcagga attaataaaa ttattccaac ttacaatgaa tttaaaaaag
     2821 caccaaaagt aaatgttggt aatttagctg acaataaaaa ctttgtagct tctgaagata
     2881 aattgaataa gattgtagat tcatcggcag ctagtaaaat tgtagataaa aactttgccg
     2941 taccagagtc aaagttagga aacattgtac cagagtacaa agaaatcaat aatcgcgtga
     3001 atgtagcaac aaacaatcca gcttcacaac aagttgataa gcattttgtt gctaaaggcc
     3061 cagaagtaaa tagatttatt acgcaaaaca aagtaaacca ccacttcatt actacgcaaa
     3121 cccactacaa gaaagttatt acttcataca aatcaacaca tgtacataaa catgtaaatc
     3181 atgcaaagga ttctattaat aaacacttta ttgttaaacc atcagaatcg cctagatata
     3241 cacatccatc tcaatcttta attatcaagc atcattttgc agttcctgga tatcacgcgc
     3301 ataaatttgt tacaccaggg catgctagca ttaaaattaa tcacttttgt gttgtgccac
     3361 aaataaatag tttcaaggta attccaccat atggtcacaa ttcacatcgt atgcatgtac
     3421 caagtttcca aaataacaca acagcaacac atcaaaatgc taaagtaaat aaagcatatg
     3481 actataaata cttctattct tataaagtag ttaaaggtgt gaagaaatat ttctcatttt
     3541 cacaatcaaa tggttataaa attgggaaac catcattaaa tatcaaaaat gtaaattatc
     3601 aatatgctgt tccaagttat agccctacac actacgttcc tgaatttaag ggtagcttac
     3661 cagcaccacg agtataaaaa ttggcactaa gtttacgaga tatgataaat acctattatt
     3721 ttaaatatag tctacaatct atgtggttgt aggctgtatt ttttgcagtt tatcaataaa
     3781 cacccatcaa caaattatac cgtttttcta ctttgaaagt tggaagtaac ataatcttaa
     3841 ataaatatat tattaattaa gataaatata atactcaaga ttattgttaa tagtttgttc
     3901 atcgcaagtt aattattgtt tctaaaatat tggtatataa ttttcaatgg cgaagaaaac
     3961 agggtgaaaa agtcggtttt taaatcaaag caaataaggg agcataaaca aatgaaaagg
     4021 aaagtattag tactaacaat gggtgtaatt tgtgcaactc aattatggca ttctaatcac
     4081 gcaaacgcat tagtatcaga gagtgttgaa actaattttg ttgtaaaaga ttcggataat
     4141 aaaaatattt tacaaaccca tactgaaatt actactgagg agaaattttc agtagtagaa
     4201 aagagtcaat taaatacact aaaatcacta tctaatgaca actacataga atatgattta
     4261 catactaatc aaacaggcat aaaaaaaggt tggttatatg gttatagtga aattgactca
     4321 tcacatttca cagaccgtga caaacgcgtt attagacgtg atcatgttaa agaagcacaa
     4381 agcttaatta atgattataa atatacgcaa aaatatgaag atctcgctaa ggcaactgca
     4441 aaagtaagta cacttagtca gtctcaccaa aattatttga ataaacaaat tgataaagtg
     4501 aataataaga tagagaaaac tgaaaaacgc taatccaaag taaattataa gttatacatc
     4561 tcgtttttaa atgacaattt atccccgtaa atattataaa
//
LOCUS       DAMZKC010000175         4547 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.177, whole genome shotgun sequence.
ACCESSION   DAMZKC010000175 DAMZKC010000000
VERSION     DAMZKC010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4547
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.177"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(164..1168)
                     /locus_tag="POB55_002292"
     CDS             complement(164..1168)
                     /locus_tag="POB55_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HDI6501236.1"
                     /translation="MSIIIEKSGLFSSFQDFGRRGYEHDGVIPCGALDTLAHEIANRL
                     VANDKNEATLEMTNKMATIRFTEPTLIALAGGNVKAYTEHMTISPYKLYLLDKGDVLK
                     FRETSYTSRVYLAVGGGFELDAWLGSNSTDFNVKIGGFKGRTLQDGDEIKLKRDYTAR
                     HHKLFENLAHTKQTDWGIDGYALSFNYMSDVFHVVKNKGTEDFKEDAIQRFVKHDYKV
                     TSKANRMGMMLEGEKIKAFYEDMPPYQTVKKGTIQIKRDGTPIILLNDHYTLGSYPQI
                     GTIASYHLTKLAQKPQGSRLKFQFIDILTAEKNLVKYSNWLNQLFHGIEYRMQLEMMK
                     "
     gene            complement(1152..1862)
                     /locus_tag="POB55_002293"
     CDS             complement(1152..1862)
                     /locus_tag="POB55_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allophanate hydrolase subunit 1"
                     /protein_id="HDI6501237.1"
                     /translation="MKIYSQGDQAIVVAIEKEVSKSLTEDLLTLRSYLIEQNYPFIIE
                     IVPSESDMMIVYDARDMIKHHNIQSPFLYMKALIESIHLNIKHDFNQQDLIEIPIVYG
                     SKYGPDLESLLKHYKIKLETFIELHSKAQYFVSMMGYSPGFPYLTGLNKKLYINHTSK
                     QKKFIPAGSVVLEGKKCGIVTTDTINDWLVIGYTPLSLFNPKESDFARLKLGDNIKFR
                     PINENELEVGAFKDVNHN"
     gene            complement(1950..2156)
                     /locus_tag="POB55_002294"
     CDS             complement(1950..2156)
                     /locus_tag="POB55_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501238.1"
                     /translation="MIIVYIVLLLILVYVNYRLVNRLLSENRIYVVRLIATITTVISF
                     ILVYALIHELMPFVVRAMDLMYHQ"
     gene            complement(2222..2830)
                     /locus_tag="POB55_002295"
     CDS             complement(2222..2830)
                     /locus_tag="POB55_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HDI6501239.1"
                     /translation="MQLFETMKIENGHIPRLTYHTNRIKCSSERLNFKFDEHAWRNEL
                     NDVTTKYHSGQYRLKIVLNAESEFETIVSPLPEKSSFTAKFQVLPKVVNPTFINNKTT
                     ERKHLAHNHETDLILLTSEDGKVLEFDIGNIVIEEDGKWYTPSYKDDFLKGCMRDYLI
                     DSDKLVEKDFNKNELIYKYHNNEIRLFLINSLREVADVHLCL"
     gene            complement(2830..3981)
                     /locus_tag="POB55_002296"
     CDS             complement(2830..3981)
                     /locus_tag="POB55_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HDI6501240.1"
                     /translation="MRIEYNYRYYLTENEYKQYHIQLKGFIKKYVATKLADVGEVIHF
                     AQAQQRQGRYVSLYLSYEAAKYFNHVMCTHSLGKDDIYAVAYSFEKAESINSTYEHQT
                     SYVSKHHFSFVESSEVMMTNIKRVQQAIVEGETYQVNYTARLTDNIYYPISTLYERLT
                     QFSNGNYTALLQTDEIQVASISPELFFQKGQFNNVDNVIISKPMKGTMPRGKTEAEDQ
                     QYYKTLQTSSKDRAENVMIVDLLRNDIGRISQSGSIKVYKLFFIEAYKTVFQMTSMVS
                     GTLKTNTDLTQILTSLFPCGSITGAPKLNTMKYIKQLESSPRGIYCGAIGLLLPTEDD
                     KMIFNIPIRTIEYKYGQAIYGVGAGITIDSKPKDEVNEFYAKTKILEML"
     gene            complement(3965..>4547)
                     /locus_tag="POB55_002297"
     CDS             complement(3965..>4547)
                     /locus_tag="POB55_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HDI6501241.1"
                     /translation="IDNNDSFTYNLIDYIKTQTKLTVQVVGIDNLLIEDVINMKPKAI
                     VISPGPGNPDDYPILNEVLEQFYQRVPILGVCLGFQCIVSYFGGNIIHGYHPVHGHTT
                     QLRHTNEGIFQGLPQNFNVMRYHSLIADGATFPNCLKITAKNDEAIIMAFEHITFPVF
                     GVQYHPESILSEYGYRQVELFLSKVGDYCENRI"
BASE COUNT         1435 a          821 c          638 g         1653 t
ORIGIN      
        1 taattttagt caaaattcaa gtaaaaacac acttcatctt ttcattttac tgtgaaacga
       61 attagaatat gatacatttc gcagataaaa cacagtatta tttcattata cattaggagg
      121 aaattatttg catcggactc gagtatgagc tacgtaccaa atattatttc atcatttcta
      181 attgcattct atattctatt ccatggaata attggtttaa ccagttacta tacttaacaa
      241 ggttcttttc agccgttaaa atatctataa attgaaattt caaacgtgat ccttgcggtt
      301 tttgtgctaa tttcgttaaa tgataacttg cgattgtacc gatttgcggg tagctaccta
      361 gcgtataatg atcatttaat aagataatag gtgtgccatc acgcttaatt tgtatcgttc
      421 cttttttgac agtctgatac ggtggcatat cttcataaaa ggctttgatt ttttcacctt
      481 caagcatcat ccccatgcga tttgctttgc tcgttacttt ataatcatgt ttcacaaatc
      541 tttgaatggc atcttcttta aaatcttccg tacctttatt tttaacgaca tgaaatacat
      601 cagacatata attaaatgac aaggcgtatc catcaatacc ccaatctgtt tgtttcgtgt
      661 gagcaaggtt ttcaaataac ttatgatgac gagctgtata atctctctta agctttattt
      721 catcgccatc ttgtaatgtt ctacctttaa aaccaccaat ttttacatta aagtcggttg
      781 agttagatcc taaccatgca tctaattcaa atccgcctcc cacagctaaa tacactcgcg
      841 atgtataact tgtttctcta aactttaaaa catcgccttt atctaacaaa tacaatttat
      901 atggagatat agtcatatgc tcagtgtaag ctttgacatt accccctgct aatgcaatca
      961 gcgtaggttc tgtaaaacga atcgttgcca ttttattagt catttccaaa gttgcttcat
     1021 tcttgtcatt tgcaactaat cgattagcaa tttcatgtgc taaagtatca agtgcaccac
     1081 atggaattac accatcatgt tcatatcccc ttctgccaaa gtcctgaaag ctactgaata
     1141 agccactttt ttcaattatg attgacatct ttaaacgctc ctacttctaa ttcattttca
     1201 ttgataggtc taaatttaat attatcgcct aactttaagc gtgcgaaatc tgattctttc
     1261 ggattaaaaa gtgataatgg tgtataacca ataactaacc aatcattaat tgtatccgta
     1321 gttacaatac cgcatttttt cccttcaagt actacagaac cagctggaat gaattttttc
     1381 tgtttactcg tgtgattaat atacaatttc ttatttaatc cagttaaata aggaaaccca
     1441 ggtgaatatc ccatcatcga aacaaaatat tgcgccttag aatgtaattc aataaaagtt
     1501 tctagcttga ttttgtaatg ttttaaaagt gattctaaat ccggaccata tttcgaacca
     1561 tacacaattg gtatttcaat caaatcttgc tggttaaaat catgttttat gtttaaatga
     1621 atcgattcta ttagtgcttt catgtataaa aaaggtgatt gtatattatg gtgtttaatc
     1681 atatcccttg cgtcatagac aatcatcatg tctgattctg atggcacaat ttctataata
     1741 aatggataat tttgttcaat taaatatgag cgaagtgtta ataaatcttc ggttaaactt
     1801 ttagatactt ctttttcaat tgcgactaca atggcttggt caccttgact atatattttc
     1861 atttgtacac ctcttcaata tttggttact ttactttcat ttttaattat agaatcgatt
     1921 gaatagttca ttttacctac tcactaaatt tactggtgat acattaaatc cattgcccgc
     1981 acaacaaaag gcatgagttc gtgaattaat gcgtatacaa ggataaagct tataacagta
     2041 gtaattgttg ctatcaaacg aacaacatat attctatttt cagatagcaa tcgattcact
     2101 aatcgataat ttacgtatac aagaattaac aacagcacaa tataaacaat aatcatattt
     2161 ggccccattt aatttttaaa tttgttttca catcattttc tacattatta tcataataat
     2221 tttaaaggca aaggtggaca tcggcaacct ctcgtaaact atttatcaaa aataaacgta
     2281 tctcattgtt atgatattta taaatcaatt cgtttttatt aaagtctttt tcaacaagtt
     2341 tgtcactatc tattaaataa tcacgcatgc atccttttaa gaaatcatct ttataacttg
     2401 gtgtgtacca ttttccatcc tcttcaatga caatgttgcc aatatcaaat tcaaggacct
     2461 tgccgtcctc tgaagttagc aatattaaat ctgtttcatg attgtgagct aaatgctttc
     2521 gttctgtcgt tttattattt ataaaagttg gattaactac tttgggcaac acttgaaatt
     2581 ttgctgtaaa actacttttc tcaggtaaag gtgacactat cgtttcaaat tcgctttcag
     2641 catttaatac gattttaagt ctatattggc cactgtgata ctttgttgtt acatcgttta
     2701 attcatttcg ccatgcatgt tcatcaaatt taaagtttaa tcgctcagaa gaacatttta
     2761 tgcgattagt atgataagta agtctaggga tatgtccatt ttcaattttc attgtttcaa
     2821 ataattgcat tataacatct ccaaaatctt ggtttttgcg taaaattcat tcacttcatc
     2881 ttttggctta gaatcaattg taatacctgc tccgactcca taaatcgctt gtccatattt
     2941 atactcaata gtacgaatcg gaatattaaa aatcatttta tcatcttcag ttggaagtaa
     3001 tagtccaatt gctccgcagt atataccacg aggtgaactt tctaattgtt taatatattt
     3061 cattgtattc agtttcggtg cacctgtaat cgaaccacaa ggaaataacg atgttaagat
     3121 ttgagttagg tctgtattag tttttaaagt accacttacc atcgaagtca tttgaaatac
     3181 agttttatat gcctcaataa aaaatagttt atacacctta attgagccac tctgtgatat
     3241 tctccctata tcgtttctta gtaaatcaac aatcatgaca ttttctgcac gatctttcga
     3301 agaagtttgc aatgttttat aatactgttg atcttcagct tccgttttac ctctaggcat
     3361 tgtccctttc atcggtttgc ttatgataac gttatcgaca ttgttaaact gtcctttttg
     3421 aaaaaataat tctggtgaga tagacgctac ttggatttca tctgtttgta ataacgcagt
     3481 ataattacca ttactaaatt gagttaatcg ttcatataaa gtactaatag gataataaat
     3541 gttatccgtt aagcgcgccg tatagttcac ttgatacgtt tcgccttcaa caattgcttg
     3601 ttggacacgt ttaatattag tcatcataac ctcagaagat tcaacaaatg aaaaatgatg
     3661 ctttgataca taagaagttt gatgttcata tgttgaattt atgctttccg ctttttcaaa
     3721 actataagct actgcataaa tatcatcttt acctaatgaa tgtgtacaca taacatgatt
     3781 aaaatacttt gccgcttcgt aacttaaata taacgagaca tatctacctt gtcgttgctg
     3841 cgcttgtgca aagtgtatca cttctcccac atcagccaac ttagtagcaa catacttctt
     3901 tataaatccc tttaattgaa tatggtattg cttatattca ttttcagtta aatagtagcg
     3961 ataattatat tctattctca cagtaatcac ctaccttcga taaaaataat tcaacttgtc
     4021 gataaccgta ttcactcaaa atagattcag gatgatattg cacaccaaaa accggaaatg
     4081 taatatgctc aaatgccata ataatcgctt catcgttttt ggctgtaatc tttaagcaat
     4141 ttggaaaagt cgctccgtca gcaattaatg aatgataacg cattacattg aaattttgag
     4201 gcagtccttg aaaaatacct tcattggtat ggcgtaactg tgtagtatgt ccgtgtacag
     4261 gatgatagcc gtgaatgata tttccaccaa aataagacac gatacattga aatcctaagc
     4321 atacacctag tataggtaca cgctgataaa attgttctaa cacttcattc aatataggat
     4381 aatcatccgg attacccggc ccaggtgaaa taacaattgc ttttggcttc atattaatga
     4441 cgtcttctat cagcagatta tcaataccaa caacttgaac tgttagtttc gtttgagtct
     4501 taatatagtc tattaaatta tatgtaaatg aatcattatt atctatg
//
LOCUS       DAMZKC010000176         4451 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.178, whole genome shotgun sequence.
ACCESSION   DAMZKC010000176 DAMZKC010000000
VERSION     DAMZKC010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4451
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.178"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            55..996
                     /gene="pde2"
                     /locus_tag="POB55_002298"
     CDS             55..996
                     /gene="pde2"
                     /locus_tag="POB55_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pApA hydrolase Pde2"
                     /protein_id="HDI6501242.1"
                     /translation="MISTLNEIMKCIEDNDTIIIHRHVRPDPDAYGSQLGLKYYIQQK
                     FPQKQVFAVGEAESSLSFIGELDNIDDKTYQDALVIVCDTANAPRIDDERYSTGRKLI
                     KIDHHPAVDQYGDINLVNTNASSTSEIIYDLISHFNDEAIVNKDIASVLYLGIVGDTG
                     RFLFNNTSEHTMEIAGKLIGHDIDHNALLNKMMEKDPKMLPFQGYVLQHFELMDDGFC
                     QVKITEDVLEQFGIQPNEASQFVNTIADIKGLKIWVFAVDEGNEIRCRLRSKGQLIIN
                     DIAQDFGGGGHPNASGVSVDSWDEFEQLATALRTKLN"
     gene            1017..4214
                     /locus_tag="POB55_002299"
     CDS             1017..4214
                     /locus_tag="POB55_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDI6501243.1"
                     /translation="MVAYLNIHTAYDLLNSSLKIEDAVRLAVSENVDALAITDTNVLY
                     GFPKFYDACIANNIKPIFGMTIYVTNGLNTVETVVLAKNNDGLKDLYQLSSEIKMNAL
                     EHVSFELLKRFSNNMIIIFKKVGDQHRDIVQVFETHNDTYMDHLSISIQGRKHVWIQN
                     VCYQTRQDADTISALAAIRDNTKLDLIHDQEDFGAHFLTEKEINQLDINQEYLTQVDV
                     IAQKCDAELKYHQSLLPQYETPNDESAKKYLWRVLVTQLKKLELNYDVYLERLKYEYK
                     VITNMGFEDYFLIVSDLIHYAKTNDVMVGPGRGSSAGSLVSYLLGITTIDPIKFNLLF
                     ERFLNPERVTMPDIDIDFEDTRRERVIQYVQEKYGELHVSGIVTFGHLLARAVARDVG
                     RIMGFDEVTLNEISSLIPHKLGITLDEAYQIDDFKKFVHRNHRHERWFSICKKLEGLP
                     RHTSTHAAGIIINDHPLYEYAPLTKGDTGLLTQWTMTEAERIGLLKIDFLGLRNLSII
                     HQILTQVKKDLGINIDIEKIPFDDQKVFELLSQGDTTGIFQLESDGVRSVLKKLKPEH
                     FEDIVAVTSLYRPGPMEEIPTYITRRHDPSKVQYLHPHLEPILKNTYGVIIYQEQIMQ
                     IASTFANFSYGEADILRRAMSKKNRAVLESERQHFIEGAKQNGYHEDISKQIFDLILK
                     FADYGFPRAHAVSYSKIAYIMSFLKVHYPNYFYANILSNVIGSEKKTAQMIEEAKKQG
                     ITILPPNINESHWFYKPSQEGIYLSIGTIKGVGYQSVKVIVDERYQNGKFKDFFDFAR
                     RIPKRVKTRKLLEALILVGAFDAFGKTRSTLLQAIDQVLDGDLNIEQDGFLFDILTPK
                     QMYEDKEELPDALISQYEKEYLGFYVSQHPVDKKFVAKQYLTIFKLSNAQNNKPILVQ
                     FDKVKQIRTKNGQNMAFVTLNDGIETLDGVIFPNQFKKYEELLSHNDLFIVSGKFDHR
                     KQQRQLIINEIQTLATFEEQKLAFAKQIIIRNKSQIDMFEEMIKATKENANDVVLSFY
                     DETIKQMTTLGYINQKDSMFNNFIQSFNPSDIRLI"
BASE COUNT         1598 a          605 c          818 g         1430 t
ORIGIN      
        1 tgatatagac taatgcttaa attaagcaac ttttctaaaa aagggagtat gaaaatgatt
       61 agtactttga atgaaattat gaaatgtatc gaagacaacg atacaattat tatacacaga
      121 catgttagac cagatcctga tgcatatggt tcacaacttg gtttaaaata ctatattcag
      181 cagaaatttc cgcaaaagca agtatttgca gtgggtgaag cggaatcatc attaagtttt
      241 attggagagt tagataacat tgatgataaa acatatcaag atgcgcttgt aattgtatgt
      301 gatactgcca atgctccacg aattgacgat gaacgttata gtacaggtcg taaacttatt
      361 aaaatagatc atcatcctgc agttgatcag tatggtgata ttaatttagt taatacgaac
      421 gcgtcatcta caagtgaaat catttacgat ttaatctcac attttaatga tgaagcaatt
      481 gttaataaag atatcgcgag cgttttatat cttggtatcg tcggtgatac tgggcgattc
      541 ctttttaaca atacctcaga acatactatg gaaattgctg gaaagttaat tgggcatgat
      601 attgatcata atgcgctttt aaataaaatg atggagaagg acccaaaaat gttgccgttt
      661 caaggttatg ttttacaaca tttcgaactt atggatgatg gattctgcca agttaaaata
      721 actgaagatg tattggagca attcggtatt cagccaaatg aagcatctca gtttgttaat
      781 acaattgctg acatcaaagg cttgaaaata tgggtatttg cagtcgatga aggtaatgaa
      841 atcagatgtc gattacgttc taaagggcaa ttgattatta atgatattgc gcaagatttt
      901 ggtggcggtg gtcatccgaa tgcgtcagga gtttcagtgg acagctggga tgaatttgag
      961 caacttgcta cagctttacg cacaaaactt aactaataga aaggggccat tcaatcatgg
     1021 tggcatattt aaatattcat acggcttatg atttgttaaa ttcaagctta aaaatagaag
     1081 atgccgtaag acttgctgtg tctgaaaatg ttgatgcact tgccataact gacaccaatg
     1141 tattgtatgg ttttcctaaa ttttatgatg catgtatagc aaataacatt aaaccgattt
     1201 ttggtatgac aatatatgtg acaaatggat taaatacagt cgaaacagtt gttctagcta
     1261 aaaataatga tggattaaaa gatttgtatc aactatcatc ggaaataaaa atgaatgcat
     1321 tagaacatgt gtcgtttgaa ttattaaaac gattttctaa caatatgatt atcattttta
     1381 aaaaagtcgg tgatcaacat cgtgatattg tacaagtgtt tgaaacccat aatgacacat
     1441 atatggacca ccttagtatt tcgattcaag gtagaaaaca tgtttggatt caaaatgttt
     1501 gttaccaaac acgtcaagat gccgatacga tttctgcatt agcagctatt agagacaata
     1561 caaaattaga cttaattcat gatcaagaag attttggtgc acatttttta actgaaaagg
     1621 aaattaatca attagatatt aaccaagaat atttaacgca ggttgatgtt atagctcaaa
     1681 agtgtgatgc agaattaaaa tatcatcaat ctctacttcc tcaatatgag acacctaatg
     1741 atgaatcagc taaaaaatat ttgtggcgtg tcttagttac acaattgaaa aaattagaac
     1801 ttaattatga cgtctattta gagcgattga aatatgagta taaagttatt actaatatgg
     1861 gttttgaaga ttatttctta atagtaagtg atttaatcca ttatgcgaaa acgaatgatg
     1921 tgatggtagg tcctggtcgt ggttcttcag ctggctcact ggtcagttat ttattgggaa
     1981 ttacaacgat tgatcctatt aaattcaatc tattatttga acgtttttta aacccagaac
     2041 gtgtaacaat gcctgatatt gatattgact ttgaagatac acgccgagaa agggtcattc
     2101 agtacgtcca agaaaaatat ggcgagctac atgtatctgg aattgtgact ttcggtcatc
     2161 tgcttgcaag agcagttgct agagatgttg gaagaattat ggggtttgat gaagttacat
     2221 taaatgaaat ttcaagttta atcccacata aattaggaat tacacttgat gaagcatatc
     2281 aaattgacga ttttaaaaag tttgtacatc gaaaccatcg acatgaacgc tggttcagta
     2341 tttgtaaaaa gttagaaggt ttaccaagac atacatctac acatgcggca ggaattatta
     2401 ttaatgacca tccattatat gaatatgccc ctttaacgaa aggggataca ggattattaa
     2461 cgcaatggac aatgactgaa gccgaacgta ttgggttatt aaaaatagat tttctaggtt
     2521 tgagaaactt atcgattatt catcaaatct taacacaagt caaaaaagat ttaggtatta
     2581 atattgatat cgaaaagatt ccgttcgatg atcaaaaagt gtttgaattg ttgtcgcaag
     2641 gagatacgac tggcatattc caattagagt ctgacggtgt aagaagtgta ttaaaaaaat
     2701 taaagccgga acactttgaa gatattgtgg ctgtaacttc tttgtataga ccaggtccaa
     2761 tggaagaaat tccaacttac attacaagaa gacatgatcc aagcaaagtt caatatttac
     2821 atccgcattt agaaccgata ttaaaaaata cttacggtgt tattatttat caagagcaaa
     2881 ttatgcaaat agcgagcaca tttgcaaact tcagttatgg tgaagcggat attttaagaa
     2941 gagcaatgag taaaaaaaat agagctgttc ttgaaagtga gcgtcaacat tttatagaag
     3001 gtgcaaagca aaatggttat cacgaagaca ttagtaagca aatatttgat ttgattctga
     3061 aatttgcaga ttatggtttt cctagagcac atgctgtcag ctattctaaa attgcataca
     3121 ttatgagctt tttaaaagtc cattatccaa attattttta cgcaaatatt ttaagtaatg
     3181 ttattggaag tgagaagaaa actgctcaaa tgatagaaga agcaaaaaaa caaggtatca
     3241 ctatattgcc accgaacatt aacgaaagtc attggtttta taaaccttcc caagaaggca
     3301 tttatttatc aattggtaca attaaaggtg ttggttatca aagtgtgaaa gtgattgttg
     3361 atgaacgtta tcagaacggc aaatttaaag atttctttga ttttgctaga cgtataccga
     3421 agagagtcaa aacgagaaag ttacttgaag cactgatttt agtgggagcg tttgatgctt
     3481 ttggtaaaac acgttcaacg ttgttgcaag ctattgatca agtgttggat ggcgatttaa
     3541 acattgaaca agatggtttt ttatttgata ttttaacgcc aaaacagatg tatgaagata
     3601 aagaagaatt gcctgatgca cttattagtc agtacgaaaa agaatattta ggattttatg
     3661 tttcgcaaca cccagtagat aaaaagtttg ttgccaaaca atatttaacg atatttaaat
     3721 tgagtaacgc gcagaataat aaacctatat tagtacagtt tgataaagtt aaacaaattc
     3781 gaactaaaaa tggtcaaaat atggcattcg tcacattaaa tgatggcatt gaaactttag
     3841 atggtgtgat tttccctaat cagtttaaaa agtacgaaga gttgttatca cataatgact
     3901 tgtttatagt tagcgggaaa tttgaccata gaaagcaaca acgtcaacta attataaatg
     3961 agattcagac attagccact tttgaagaac aaaaattagc atttgccaaa caaattataa
     4021 ttagaaataa atcacaaata gatatgtttg aagagatgat taaagctacg aaagagaatg
     4081 ctaatgatgt tgtgttatcc ttttatgatg aaacgattaa acaaatgact actttaggct
     4141 atattaatca aaaagatagt atgtttaata attttataca atcctttaac cctagtgata
     4201 ttaggcttat ataactttta tcaactaata aattatgata tagtaaactg atggttagat
     4261 attttctaac catcagtttg cgtttatatt agttttttat gcttattatt tttatgagtt
     4321 tcactttaca ttattgatta atccataaga ataattagca caaaaagcag tatacataaa
     4381 ttgagtaaag aattttgtcg atatatcgtg cggaaaaata ataacttttt gatatattgg
     4441 tttttgctat c
//
LOCUS       DAMZKC010000177         4367 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.179, whole genome shotgun sequence.
ACCESSION   DAMZKC010000177 DAMZKC010000000
VERSION     DAMZKC010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4367
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.179"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            136..327
                     /locus_tag="POB55_002300"
     CDS             136..327
                     /locus_tag="POB55_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501244.1"
                     /translation="MRKEIEALIFSDVSSYDIYVNTGVNQGLIGDIKDGYLTIDSMPY
                     IDAERLYHFAMERKSLVTN"
     gene            complement(466..651)
                     /locus_tag="POB55_002301"
     CDS             complement(466..651)
                     /locus_tag="POB55_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501245.1"
                     /translation="MLYILITALNVILNKLKMKDSNIISFIYSRHDRILYVNLVGNRF
                     KNNISMTKATSNIGRGF"
     gene            complement(587..868)
                     /locus_tag="POB55_002302"
     CDS             complement(587..868)
                     /locus_tag="POB55_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDI6501246.1"
                     /translation="MTYHERVLALRAESKRTAFDFRFEDLFSKEEWLSMSLAERQKAE
                     KAFRHEVKNMDDVRMPFSSVHDAQVKLYNVVYSYNGIKRNFKQVENEGF"
     gene            1157..1852
                     /locus_tag="POB55_002303"
     CDS             1157..1852
                     /locus_tag="POB55_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDI6501247.1"
                     /translation="MTVLTDKIAVVTGAGSGIGEAIATLLHEEGAKVVLAGRNKEKLQ
                     NVANQLSQDSVKVVPTDVTNKEEVDELIKIAQQTFGGLDIVINSAGQMLSSKITDYQV
                     DEWDSMIDVNIKGTLYTVQAALPTMLEQSSGHLINIASISGFEVTKSSTIYSATKAAV
                     HTITQGLEKELAKTGVKVTSISPGMVDTAITAAYNPSDRKKLDPQDIAEAVLYALTQP
                     SHVNVNEITVRPV"
     gene            2103..2525
                     /locus_tag="POB55_002304"
     CDS             2103..2525
                     /locus_tag="POB55_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HDI6501248.1"
                     /translation="MNKKHVFVIIGVILCICIVATVIYLKVKYDEKEKQKAIYYKEQQ
                     ERITLYLKHNTKEPNTIKSVHFTSLKRGPMGDAVIEGYINENKKADFVAYGSPEHNYQ
                     FGGSLIKSKNLSTLLKPAHQTKTPDDIKKELDSKKNDR"
     gene            2793..3203
                     /locus_tag="POB55_002305"
     CDS             2793..3203
                     /locus_tag="POB55_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HDI6501249.1"
                     /translation="MSKKKILILTSIMLIILISVASIYFKMKYDEKEKQKAIYYKEQQ
                     ARITLYLKHNTKEPNTIKSVHFTKLETSPMGSAVIEGYINENKKDDFVAYVSPENNFQ
                     FVGDLIKSERLSELLKPANQLKSPDDIKKELNKK"
     gene            3230..3649
                     /locus_tag="POB55_002306"
     CDS             3230..3649
                     /locus_tag="POB55_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HDI6501250.1"
                     /translation="MNKKHVFVIIGVILCICIVVTAIHFKIKYDEKEKQKAIYYKEQQ
                     ERITLYLKHNTKEPNTIKTVHFTNFETSPMGSAVIEGYINENKKDNFTAYATPEHNYQ
                     FGGAMIESERLSELLKPAHQLKSPDDIKKELNKKKSH"
     gene            3667..4086
                     /locus_tag="POB55_002307"
     CDS             3667..4086
                     /locus_tag="POB55_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HDI6501251.1"
                     /translation="MSKKHGFIIIGVILCICIVASVIYLKMKYDEKEKQKALYYKEQQ
                     ERITLYLKHNTKEPNTIKSVHFTSLKRGPMGDAVIEGYINENKKADFVAYGSPEHNYQ
                     FGGTMIESERLSELLKPANQLKSPEKIKEELDKKEGH"
BASE COUNT         1652 a          631 c          730 g         1354 t
ORIGIN      
        1 ataaatttgt gaaatacatc acaaaaccct ttatttattt ggaaattcat gtaatattag
       61 tcttgtaaga agttaataaa tagagagaga cgagagagtt tatataaata atatataaac
      121 attggagtga tgattatgag aaaagagatt gaagcgctta ttttctcaga cgtaagtagc
      181 tatgatattt acgtgaacac tggtgtaaat caaggattaa ttggtgacat caaagatggt
      241 tacctaacta ttgattctat gccttacatt gatgctgagc gtttgtatca ctttgctatg
      301 gaacgtaaat cgttagtcac taactagttc ttattgccaa tgattactac ccctagtctg
      361 cggcaattga agtgtgattg atgtaacttg ccctcgttgg tgagcaattg agggcagacc
      421 cctttaatta agtaaaccct aactccccac aaatctggaa cgatactaaa agccacgtcc
      481 tatattggat gtggctttag tcatgcttat attattttta aaacgattac ctacaagatt
      541 tacatataaa attctatcat gtctgctata tataaacgaa atgatattag aatccttcat
      601 tttcaacttg tttaaaatta cgtttaatgc cgttataaga atatacaaca ttatataatt
      661 ttacttgggc gtcatggaca cttgagaagg gcattcttac atcgtccata tttttaactt
      721 cgtgtcgaaa tgctttttca gctttttgtc tttctgcaag cgacatactt agccattctt
      781 ctttgctaaa taaatcttcg aatcgaaaat caaatgcggt tcttttactt tcagctctta
      841 atgctaaaac acgctcatga tatgtcatgt tttattcctc cctctaatca ttcaatcgaa
      901 tgataaatca cagtattaac tttagtacta atataatgtt tttacaataa agaagtgacg
      961 atttttcaac atttttaaaa ataaaaaata taagcaacga ttaattgtat atttcgaatg
     1021 gcttggaata tactttattc acgaaaatga agcgagttat tatgaccgtt atatgaattg
     1081 tgatacgctt tttaaaagta atatgtattt atgatttacc ctgaaaaaat aaaaagaatc
     1141 aaaggagtcg atcattatga cagtattaac agataaaata gcagtagtta caggtgcagg
     1201 tagtggtatt ggagaagcaa ttgcaacatt actacatgaa gaaggggcga aagttgtctt
     1261 agcaggtaga aataaagaaa aattacaaaa cgtagcgaat caattgtcac aagatagtgt
     1321 gaaggtagtg ccaacagatg taacgaataa agaagaagtc gatgaattga taaaaattgc
     1381 acaacaaaca ttcggtggtt tggatattgt tatcaatagt gcggggcaaa tgttgtcgtc
     1441 taagattact gattatcaag tagatgagtg ggatagtatg attgatgtga atatcaaagg
     1501 cactttatat acggtacagg ctgcattacc aactatgtta gaacaatcaa gtggccatct
     1561 tattaacatt gcatctattt ctggctttga agtaacgaaa agtagtacga tttatagtgc
     1621 gacgaaagca gcagttcaca ctattactca aggattagaa aaagagttgg caaagacagg
     1681 tgttaaagta acaagcattt caccaggaat ggtagataca gcaataactg ccgcatataa
     1741 tccaagtgat cgtaaaaagc ttgatccaca agatattgca gaagcggtat tgtatgcatt
     1801 aacacaaccg agtcacgtta acgtgaatga aattacagtg cgtcctgtat aaaaatgtgt
     1861 ttgaggtgtg ggagatagac atttaatgta atagctatcg gttttgttaa tattagtaag
     1921 gcaataatga tataaaagca tgataggcaa ttattccttg cacaattttc actaactggt
     1981 gattttaaca atcgagttaa tgaatggggc gatgttagtg ttatggttta taatctgtaa
     2041 tgttcataat tatagattac aaaaaatttt tcattttcca ccacatttta ggagttactt
     2101 taatgaacaa aaaacatgtt tttgtaatta ttggtgtcat attatgtata tgtatagttg
     2161 ctacagtcat ttatttaaaa gtgaaatatg acgaaaaaga aaaacaaaaa gcaatttact
     2221 ataaagaaca acaggagcgt attacacttt atctcaagca taatactaaa gaaccaaaca
     2281 cgattaaatc tgtgcatttc acaagtttaa aaagaggacc tatgggcgat gccgtaattg
     2341 aaggttacat caatgaaaac aaaaaagctg attttgttgc atatgggtct ccagaacata
     2401 attatcaatt tggtggaagt ttaatcaaaa gtaaaaattt aagcacgtta ttaaaaccag
     2461 cacatcaaac caaaacgcct gatgatataa aaaaagaact agattctaaa aagaacgacc
     2521 gctaaaacta tgcggtcgtt ttaattttgt tttcaagcta atcacactta cacaaaaaaa
     2581 taaaaacttg aatttttagt acaacattcc actgtattta tctgttaacc actttatacc
     2641 cgcaatattt cgttgatctt atataaacac ttttggctgc ttttccttga cctagattaa
     2701 aattatagat tttctttgta agagtgtgtt ttgtagtatc ataaatatgt aaactgtata
     2761 aaatcccacc acattttagg agctggtgat agatgtcgaa aaagaaaatt ttaatattaa
     2821 ctagtattat gttaatcata ttaattagtg ttgcaagtat ctattttaaa atgaaatatg
     2881 acgaaaaaga aaaacaaaaa gcaatttatt ataaagaaca acaagcgcgc attacacttt
     2941 atctcaagca taacactaaa gaaccgaaca cgattaaatc tgtgcatttc acaaaattgg
     3001 aaacaagtcc tatgggaagt gctgtgattg aaggttacat aaatgaaaac aaaaaagatg
     3061 attttgttgc ctatgtatca cctgaaaata actttcaatt tgtaggtgat ttaattaaaa
     3121 gtgaaagatt aagtgagtta ctaaaaccag caaatcaatt aaaatcacca gatgatataa
     3181 aaaaagaact aaataaaaag tagagtcact aaagttagga gttactttaa tgaacaaaaa
     3241 acatgttttt gtaattattg gtgtcatttt atgtatatgt atagttgtta cggccataca
     3301 ttttaaaatt aaatatgatg aaaaagaaaa acaaaaagca atatactata aagaacaaca
     3361 agagcgcatt acactttatc tcaagcataa taccaaggag ccaaacacaa tcaaaactgt
     3421 acatttcact aactttgaaa caagtcctat gggaagtgct gtgattgaag gctacatcaa
     3481 tgaaaacaaa aaagataatt ttactgctta tgctacacct gaacataatt atcagtttgg
     3541 tggtgctatg atagaaagtg aaagattaag tgagttacta aaaccagcgc atcaattaaa
     3601 atcaccagat gatataaaaa aagaactaaa taaaaagaag agtcactaaa gttaggagtt
     3661 gctttaatgt ccaaaaaaca tggttttata attattggtg tcattttatg tatatgtata
     3721 gttgcatccg tcatttattt aaaaatgaaa tatgacgaaa aagaaaaaca aaaagcactt
     3781 tactacaaag aacaacagga gcgtattaca ctctatctca agcataatac caaagaacca
     3841 aacacgatta aatctgtgca tttcacaagt ttaaaaagag gacctatggg cgatgccgta
     3901 attgaaggtt acatcaatga aaacaaaaaa gctgattttg ttgcatatgg gtctccagaa
     3961 cataattatc agtttggtgg tactatgata gaaagtgaaa gattaagtga gttactaaaa
     4021 ccagcaaatc aattaaaatc tcctgaaaaa attaaagaag aattagacaa aaaagaaggc
     4081 cactagggtc ttctttattt ttgatttaat cttccaataa tctatgtcat tgctatcgaa
     4141 ggtgtttcgc aattaatatc aatcacttca tcatcaccaa tacttcccca gttttgtaca
     4201 gtacattaac acaaacgaac cacgttaatt taaatggaat aatagtttgg ccattataag
     4261 aacaatatat atcgaataac aatgtcgatg acgtggttta ttgacattat caaattttct
     4321 caaatgtgtg tgatttttta taaaaaaggg tagagcaagg tataata
//
LOCUS       DAMZKC010000178         4365 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.180, whole genome shotgun sequence.
ACCESSION   DAMZKC010000178 DAMZKC010000000
VERSION     DAMZKC010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4365
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.180"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            114..2945
                     /gene="fnbB"
                     /locus_tag="POB55_002308"
     CDS             114..2945
                     /gene="fnbB"
                     /locus_tag="POB55_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein FnbB"
                     /protein_id="HDI6501252.1"
                     /translation="MKSNLRYGIRKHKLGAASVFLGTMIVVGMGQEKEAAASEQNNTT
                     VEESGSSATERKASETQTTTNNVNTIDETQSYSATSTEQPSQSTQVTTEEAPTTVQAP
                     KVETSRVDLPSEKVADKETTGTQVDTAQPSNVSEIKPRMKRSTDVTAVTEKEVVEEAK
                     ATGTDVTSKVEVEEGSEIVGHNNKETNVVNPHNAERVTLKYKWKFEDGIKPGDYFDFT
                     LSNNVETHGISPLRKVPDIKSKDDNILAVGKVMDERKIRYTFTDYINNKNNLMAELNL
                     NLFIDPTTVTKQGKQTVEVKLGENKISKEFDIKYLDGVKDNWGVTVNGRITLLDKENS
                     KIHHLAYINPKKSDMTSITINGFFAKGGLYTGNVPTVKVYEYLRSDELPESVYANTND
                     QEKFKDVTNDMSDKLTLSENGSYKLTLDALNKKSYVVSFEGKYNENDKELLFRTNLHG
                     YHANYGYYYYYPVSLTWDNGVAFYSNNAQGDGKDKPNDPIIEKSEPIELDIKSEPPVE
                     KHELTGTIEESNDSKPIDFEYHTAVEGVEGHAEGIIETEEDSIHVDFEESTHENSKHH
                     ADVVEYEEDTNPGGGQVITESNLVEFDEESTKGILTGAVSDHTTVEDTKEYTTESNLI
                     ELVDELPEEHGQAQGPIEEITENNHHISHSGLGTENGHGNYGVIEEIEENSHVDIKSE
                     LGYEGGQNSGNQSFEEDTEEDKPKYEQGGNIVDIDFDSVPQIHGQNKGDQSFEEDTEK
                     DKPKYEHGGNIIDIDFDSVPHIHGFNKHTEIIEEDTNKDKPNYQFGGHNSVDFEEDTL
                     PQVSGHNEGQQTIEEDTTPPTPPTPEVPSEPETPTPPTPEVPSEPETPTPPTPEVPAE
                     PGKPVPPAKEEPKKPSKPVEQGKVVTPVIEINEKVKAVAPTKKAQSKKSELPETGGEE
                     STNKGILFGGLFSILGFALLRRNKKNHKA"
     gene            3125..3991
                     /gene="galU"
                     /locus_tag="POB55_002309"
     CDS             3125..3991
                     /gene="galU"
                     /locus_tag="POB55_002309"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDI6501253.1"
                     /translation="MKKIKKAIIPAAGLGTRFLPATKAMPKEMLPILDKPTIQYIVEE
                     AARAGIEDIIIVTGRHKRAIEDHFDSQKELEMVLKEKGKSELLEKVQYSTELANIFYV
                     RQKEQKGLGHAISSARQFIGNEPFAVLLGDDIVESEVPAVKQLIDVYEETGHSVIGVQ
                     EVPEADTHRYGIIDPLTKNGRQYEVKKFVEKPAQGTAPSNLAIMGRYVLTPEIFDYLK
                     TQKEGAGNEIQLTDAIERMNNDNQVYAYDFEGERYDVGEKLGFVKTTIEYALKDDSMR
                     EELTRFIKELGL"
     gene            complement(4203..>4365)
                     /locus_tag="POB55_002310"
     CDS             complement(4203..>4365)
                     /locus_tag="POB55_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDI6501254.1"
                     /translation="SVNRLNNKGMLIKERSLADERIVLIKINKIQYNTIKSIFTDTSK
                     ILKPRKFFF"
BASE COUNT         1718 a          634 c          859 g         1154 t
ORIGIN      
        1 ttaacttttt attaactcgc tttttttcat tgcttttaaa aaccgaacaa tatagaattg
       61 catttattga gttaaaaaat aaatgaattt tacatttaag ggagaatatt atagtgaaaa
      121 gcaatcttag atacggcata agaaaacaca aattgggagc ggcctcagta ttcttaggaa
      181 caatgatcgt tgttggaatg ggacaagaaa aagaagctgc agcatcggaa caaaacaata
      241 ctacagtaga ggaaagtggg agttcagcta ctgaaagaaa agcaagcgaa acacaaacaa
      301 ctacaaataa cgttaataca atagatgaaa cacaatcata cagcgcgaca tcaactgagc
      361 aaccatcaca atcaacacaa gtaacaacag aagaagcacc aacaactgtg caagcaccaa
      421 aagtagaaac ttcgcgagtt gatttgccat cggaaaaagt tgctgataag gaaactacag
      481 gaactcaagt tgacacagct caaccaagta acgtctcaga aattaaacca agaatgaaaa
      541 gatcaactga tgttacagca gttacagaga aagaagtagt ggaagaagct aaagcgacag
      601 gtacagatgt aacaagtaaa gtggaagtag aagaaggtag tgaaatagta gggcataaca
      661 ataaagagac aaatgttgtt aatcctcata atgcagaaag agtaaccttg aaatataaat
      721 ggaaattcga ggatggtatt aaaccggggg actactttga tttcacatta agtaataatg
      781 tagaaactca tggtatctcg cctcttcgta aagttccaga tataaaaagt aaagatgata
      841 atattttggc tgttggcaaa gtgatggatg aaagaaaaat tagatatacg tttactgatt
      901 atataaataa caaaaataat ttaatggctg aattaaactt aaatttgttt attgatccaa
      961 ctacagttac gaagcaagga aagcaaactg ttgaggttaa attaggtgaa aataagattt
     1021 caaaagaatt tgatatcaaa tatctagacg gtgttaaaga taactgggga gtaactgtaa
     1081 atggacgtat aactttatta gataaagaaa atagtaaaat acatcattta gcttatataa
     1141 accctaaaaa aagcgatatg acctcaataa ctataaatgg gttttttgct aaaggtggat
     1201 tgtatacagg aaatgttcca acagtgaaag tttatgagta tttgagaagt gatgaattac
     1261 cagaaagcgt ctatgctaac acaaatgatc aagagaaatt taaagatgtt accaatgaca
     1321 tgagtgataa attaacgcta agtgaaaacg gtagctataa attaacttta gatgcactaa
     1381 ataagaaaag ttatgttgta agttttgaag gtaaatataa tgaaaatgac aaggagttat
     1441 tattcagaac taatttgcat ggctatcatg ctaattacgg ttattactat tattatccag
     1501 taagtttaac atgggacaat ggcgttgcat tctactctaa taacgctcaa ggcgacggta
     1561 aagataagcc aaatgatcct atcattgaga agagtgaacc aattgaatta gacattaaat
     1621 cagagccgcc agtggagaag catgaattga ctggtacaat cgaagaaagt aacgattcta
     1681 agccaattga ttttgaatat catacagctg ttgaaggtgt agaaggtcat gcagaaggta
     1741 ttattgaaac tgaagaagat tctattcatg tggattttga agaatctaca catgaaaatt
     1801 caaaacatca cgctgatgtt gttgaatatg aagaggatac aaacccaggt ggtggccaag
     1861 taataactga gtctaactta gttgaatttg acgaagagtc tacaaaaggt attctaactg
     1921 gcgcagtgag cgaccataca acagttgaag atacgaaaga atatacgact gaaagtaatc
     1981 tgattgaact agtagatgaa ttacctgaag agcatggtca agcacaagga ccaatcgagg
     2041 aaattactga aaacaatcat catatttctc attctggttt aggaactgaa aatggtcacg
     2101 ggaattatgg tgtgattgaa gaaatcgaag aaaatagcca cgttgatatt aagagtgaat
     2161 taggttatga aggtggccaa aatagcggta accagtcatt cgaggaagac acagaagaag
     2221 ataaacctaa atatgaacaa ggtggcaata tcgtagatat cgatttcgat agtgtacctc
     2281 aaattcatgg tcaaaataaa ggtgatcagt cattcgaaga agatacagag aaagacaagc
     2341 ctaaatatga acatggtggt aatatcattg atatcgactt cgacagcgtg ccacatattc
     2401 atggattcaa taagcacact gaaattattg aagaagatac aaacaaagat aaaccaaatt
     2461 atcaattcgg tggacacaat agtgttgact ttgaagaaga tacacttcca caagtaagtg
     2521 gtcataatga aggtcaacaa acgattgaag aagatacaac gccgccaaca ccaccaacac
     2581 cagaagtacc aagtgagccg gaaacaccaa caccaccgac accagaagta ccaagtgagc
     2641 cggaaacacc aacaccacca acgccagagg taccagctga acctggtaaa ccagtaccac
     2701 ctgccaaaga agaacctaaa aagccttcta aaccagtgga acaaggtaag gtagtaacac
     2761 ctgtcattga aatcaatgaa aaggttaaag cagtggcacc aactaaaaaa gcacaatcta
     2821 agaaatctga actacctgaa acaggtggag aagaatcaac aaacaaaggt atactgttcg
     2881 gaggattgtt tagtatttta ggatttgcat tattacgcag aaataaaaag aatcacaaag
     2941 cataatcaat ctaaaattga caggtttatt tcataaatta tatgaagtaa gcctgttttt
     3001 taaaattaaa tcaaatttcc caagaaataa ttacatactt aatgacacta tgaaggcgtt
     3061 ctaattagtg ttaaaatgac gttgatacat agatttaata cttaggaaaa ggagcacatt
     3121 aactttgaaa aaaataaaaa aggcaatcat tcccgctgct ggtttaggga ctagattttt
     3181 accagcaact aaagcgatgc caaaggaaat gcttcctatc ttagataaac ccacaataca
     3241 atatatcgtt gaagaagctg caagagctgg aattgaagat attattatag tgacaggtcg
     3301 ccacaaacgc gcgattgaag atcattttga tagtcaaaaa gaattagaaa tggtgttaaa
     3361 agaaaaaggt aaatctgagt tactagaaaa agttcagtat tcaacagaac ttgcgaatat
     3421 tttttatgta aggcagaaag aacaaaaagg tttagggcat gcgattagtt ctgcgcgtca
     3481 atttatcggc aatgaaccat ttgcggtatt attaggtgac gatattgttg aatctgaagt
     3541 gccagcagtg aaacagttga ttgacgttta tgaagaaaca gggcactcag ttataggtgt
     3601 ccaagaagtt ccggaagcag atactcatcg ttatgggatt attgacccat taactaaaaa
     3661 cggacgtcag tatgaagtga aaaagtttgt tgaaaagcca gcacaaggta cggcaccatc
     3721 aaacctagct attatgggac gttatgtatt aacgcctgaa attttcgatt atttaaaaac
     3781 gcaaaaagaa ggcgcaggta acgaaattca gttaacagat gcgattgaac gtatgaataa
     3841 tgataaccaa gtgtatgcat atgacttcga aggcgaacgt tatgacgttg gagaaaaatt
     3901 aggcttcgtt aaaacaacga tagaatatgc attgaaagat gacagtatgc gagaagaatt
     3961 aacacgattt attaaagaat tagggttata acattaaaaa gcatgatact caataaaatt
     4021 ggaacacgaa attgtgaact aaattgaatg agtgtcatgc ttaatttatg tgttgtgata
     4081 tatagtaggg tattgaaata atataaaaac aaaagaaagt taattatgta tttttattta
     4141 tactaaattg agaagtatta tgattgctta tttattttat caagaggtaa atctgtttaa
     4201 atttaaaaga aaaattttct tggtttgaga atcttggaag tatctgtgaa tatgctttta
     4261 atagtgttat attgtatttt atttattttg attaacacaa tcctttcatc tgcaagggat
     4321 cgttctttga ttagcatacc tttattattt aatctattaa ctgac
//
LOCUS       DAMZKC010000179         4335 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.181, whole genome shotgun sequence.
ACCESSION   DAMZKC010000179 DAMZKC010000000
VERSION     DAMZKC010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4335
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.181"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(110..958)
                     /locus_tag="POB55_002311"
     CDS             complement(110..958)
                     /locus_tag="POB55_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDI6501255.1"
                     /translation="MNHIEISKDVKIPVLGFGVFQIPQEQTAEAVKEAIKAGYRHIDT
                     AQSYLNETEVGQGIEASGIDRSELFITTKVWIENVNYEDTIKSIERSLQRLNLDYLDL
                     VLIHQPYNDVYGSWRALEELKENGKIKAIGVSNFGVDRIVDLGIHNQIQPQVNQIEIN
                     PFHQQEEQVAALQQENVVVEAWAPFAEGKNQLFQNQLLQAIADKYNKSIAQVILRWLV
                     ERDIVVLAKSVNPERMAQNLDIFDFELTEEDKQQIATLEESNSQFFSHADPEMIKALT
                     SRELDV"
     gene            complement(1028..1444)
                     /locus_tag="POB55_002312"
     CDS             complement(1028..1444)
                     /locus_tag="POB55_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDI6501256.1"
                     /translation="MKTKEVVALMNISQDTLRYYEKVGVIPPVNRDENGYRIYNDSDL
                     NWIYLVKNLRNAGVSIESLIEFCRLAQLPKNENIQAQQKQILNKQLEELNENLKTIHD
                     ARDLLQYKIDNYDNHIAKINASDNHDDNVERLWERK"
     gene            1689..1856
                     /locus_tag="POB55_002313"
     CDS             1689..1856
                     /locus_tag="POB55_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501257.1"
                     /translation="MRKIWVIDCLYASTFFVDCLTVDSHLYTLTNFEKLNHYLIIIFI
                     AHAQHKKETCQ"
     gene            1853..4300
                     /locus_tag="POB55_002314"
     CDS             1853..4300
                     /locus_tag="POB55_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide lyase 8 family protein"
                     /protein_id="HDI6501258.1"
                     /translation="MTYRIKKWQKLSTITLLMAGVITLNGGEFRSIDKYQIAVADTNV
                     QTPDYEKLRNTWLDVNYGYDKYDEKNDAMKKKFEATENEAKKLLSEMKTESDRKYLWE
                     NSKDLDTKSADMTRTYRNIEKIAEAMKHKDTKLKIDENKKKVKDALEWLHKNAYGKEP
                     DKKVADLTSNFKNKTSRNTNLNWWDYEIGTPRALTNTLILLNDQFSNDEKKKYTAPIK
                     TFAPESDKILSSVGQPEQAKGGNLVDIAKVKLLESIIEEDKDITKNSIDAFNKVFTYV
                     QSNATGKERNGFYKDGSYIDHQDVPYTGAYGVVLLEGISQMMPMIKETPFNDKTQNDT
                     TLKSWIDDGFMPLIYKGEMMDLSRGRAISRENETSHTASATVMKSLLRLSDAMDDSTK
                     AKYKQIVKTSVKSDSSYGQNDTLSSYSDISKMKSLMEDSTISTNGLTQQLKIYNDMDR
                     VTYHNKDLDFAFGLSMTSKNVARYESINGENLKGWHTGAGMSYLYNSDVKHYRDNFWA
                     TADMKRLAGTTTLENEEPKGTDVKKSSKTFVGGTKFDDQHASIGMDFENQDKTLTAKK
                     SYFILNDKIVFLGTGIKSTDSSKNPVTTIENRKANGYTLYTDDKQTTASDNQGTNSVF
                     LESTNKPKNNIGYHFLNESKITVKKESHTGKWSDINKSQKQDSKTNQYYEVTQKHSNT
                     DSKYAYVLYPGLSKDDFNTKKDKVTVVKQDDDFHVVKDNESVWAGVNYSDSTQTFIIN
                     NTKVEVKAKGMFILKKKDDKTYECSFYNPESTNTASDIESKISMTGYSITNKNTSTSN
                     ESGVRFELQQTLNKDDN"
BASE COUNT         1533 a          685 c          717 g         1400 t
ORIGIN      
        1 tttgcttgct cctttcactt ttaagagtaa taaaataacc tgaaacatag actttaacat
       61 ccaagttcag gttagcgtaa tgtaaatgat ttactttgtt atattatgat taaacatcta
      121 attctctgct tgtaagtgct ttaatcatct ctggatcagc atgtgagaaa aattggctat
      181 tactttcttc taatgtcgca atttgttgtt tatcttcttc tgtaagttca aaatcaaata
      241 tatctaagtt ttgagccata cgttctggat tcactgattt tgcgagtaca acgatgtcac
      301 gttctactaa ccagcgtaaa ataacttgtg caattgattt gttatattta tctgcaatgg
      361 cctgcaacaa ttgattttgg aataattgat ttttaccttc tgcaaatggt gcccaagctt
      421 ctaccacaac attttcttgt tgaagtgctg caacttgttc ttcttgttga tggaatggat
      481 taatttctat ttggttcact tgtggttgaa tttgattgtg aatacctaaa tctacaattc
      541 gatcaacgcc gaagttagat acaccgattg ctttaatttt accattttct tttaactctt
      601 ctaatgcacg ccatgagcca tatacatcat tataaggttg gtgaataagc actagatcaa
      661 gatagtctaa atttaatcgt tgtaatgatc gttcgattga tttgatagta tcttcatagt
      721 ttacattttc aatccatact ttagtcgtaa tgaatagttc acttctatca atacctgaag
      781 cttcaatacc ttgacctact tcagtttcat ttaaataact ttgcgctgta tcaatatgtc
      841 tatagcccgc tttaattgct tctttaaccg cttctgctgt ttgttcttga ggaatttgaa
      901 atactccgaa gcctaatact ggtatcttta catctttact tatttcaata tgattcataa
      961 aatgaacctc ctttatcttg taagtccact ataaaccatg gagcgaactc tatagcaagc
     1021 cataggttta ctttctctcc caaagacgtt caacattgtc atcatgatta tcactagcat
     1081 tgattttagc aatatgatta tcataattat caattttata ttgtagtaaa tctctcgcat
     1141 catgaattgt ctttaagttt tcatttaatt cttcgagttg cttatttaaa atttgctttt
     1201 gctgtgcttg aatattttca tttttaggaa gctgcgctaa cctgcaaaat tcgataagcg
     1261 attcaatact gacgcctgca tttcgcaaat ttttcactaa ataaatccaa tttaaatcac
     1321 tatcattata tattctatat ccattttcat ctcgattaac aggtggaatc acaccaacct
     1381 tttcataata tcttaaagtg tcttgagata tattcatgag cgctacgact tctttagttt
     1441 tcattgcgac tatcctttca gttatgttgg tggcatctta agtaatgttg ctttatatat
     1501 taccatcctc gtttgaatac ctcttatttt attactcaaa tttaaatttg tctctttttt
     1561 aacattttac atttcatcgt ttttaattac tttaaaaatt gtataactta aatatttaaa
     1621 atgatataat cactaagatt gataatattt aattttttaa aaattatttt cgttgcaatt
     1681 tttgggtgat gcggaaaatt tgggtgatcg attgtttata tgcatcaaca ttctttgtgg
     1741 attgtttgac agtagacagt catttgtata cgttaacgaa ttttgaaaaa ttgaatcatt
     1801 atttaatcat aatattcatt gcgcacgcgc aacacaaaaa ggagacatgt caatgacata
     1861 tagaataaag aaatggcaaa aattatccac cattacgtta ttaatggctg gtgtgattac
     1921 tttgaatggt ggtgaattca gaagtattga taaatatcaa atcgctgtgg ctgatacgaa
     1981 tgttcaaacg ccagattatg aaaagttgag gaacacatgg ctggacgtta actatggtta
     2041 tgataagtat gatgagaaga atgacgcaat gaagaagaag tttgaggcta cggagaatga
     2101 ggcaaagaaa ttacttagtg agatgaaaac tgaaagtgat aggaaatact tgtgggaaaa
     2161 ctcaaaagat ttagatacga agtctgcgga tatgactcgt acctatcgta atattgagaa
     2221 aatcgcagaa gcgatgaagc ataaagatac taagttaaaa atagatgaaa acaagaagaa
     2281 agtgaaagat gcccttgagt ggctgcataa aaatgcatat ggaaaagaac cagataaaaa
     2341 agttgctgat ttgacctcaa actttaaaaa taaaacttct agaaatacca atttaaattg
     2401 gtgggattat gaaattggaa cacctagagc attaacaaat acccttatat tgctgaatga
     2461 tcaattttca aatgacgaaa agaaaaaata cactgcccct atcaaaactt tcgccccaga
     2521 aagtgacaaa atattatcat cagtaggaca acctgaacaa gctaaagggg gaaatttagt
     2581 agacattgct aaagtaaaac ttttagaaag tattatcgaa gaagataaag atataactaa
     2641 aaattcaata gatgcattta ataaagtctt cacttacgtt caaagtaatg caactggtaa
     2701 agaacgtaat ggattctata aagacggctc ttatattgat catcaagacg tcccatacac
     2761 tggtgcttat ggcgttgtac tcttagaggg gatttctcaa atgatgccga tgataaaaga
     2821 aacacctttt aatgataaaa cccaaaatga tacaacctta aagtcatgga ttgacgacgg
     2881 atttatgcca ctcatttata aaggtgaaat gatggattta tcaagaggta gagctatcag
     2941 tcgtgaaaat gaaacgagtc atacagcgtc agcgactgta atgaaatcat tgttgagatt
     3001 gagtgatgcc atggatgatt caacaaaagc taagtataag caaatcgtta aaacttctgt
     3061 taaatctgat tcaagttacg gccaaaatga tacattaagc tcttattcgg atataagcaa
     3121 aatgaagtct ttaatggaag acagcactat ttctactaac ggtttaacac aacaacttaa
     3181 aatatataat gacatggatc gtgtcaccta tcacaataaa gacttagact ttgcatttgg
     3241 tttaagtatg acgtcgaaaa acgtcgcacg ttacgaaagt atcaacggag agaacttaaa
     3301 aggttggcac actggtgctg gaatgtctta tttatacaat agcgatgtga aacactaccg
     3361 tgataacttc tgggcgacag ctgatatgaa acgtttagca ggtacaacaa ctttagaaaa
     3421 tgaagaacct aaaggtacgg atgttaaaaa gtcgagtaaa acttttgtag gcggaacaaa
     3481 attcgatgac caacatgcta gtatcggaat ggattttgaa aatcaggaca aaactttaac
     3541 tgccaaaaaa tcatatttca tattaaacga taaaattgtc ttcttaggaa ctggcattaa
     3601 aagtactgat tcatcaaaga atccagttac aacgattgaa aatcgcaaag cgaatgggta
     3661 tacgttatat acagacgata aacaaacaac cgcttccgat aatcagggaa ccaattccgt
     3721 ctttttagag tcaaccaata aacctaaaaa caatataggc tatcattttt taaacgaatc
     3781 gaaaataact gtaaaaaaag aaagtcatac tggtaagtgg agtgatataa ataaaagtca
     3841 aaagcaagac agtaaaacta atcaatatta tgaagtaact caaaagcatt ctaatacaga
     3901 tagtaaatat gcatatgtgt tgtatccagg cttatctaaa gatgacttta atacaaaaaa
     3961 agataaagta actgtcgtta aacaagatga tgacttccac gttgtgaaag ataatgaatc
     4021 ggtttgggct ggtgttaatt acagcgatag cactcaaact tttatcatta acaacactaa
     4081 agtcgaagtt aaagcaaaag gtatgtttat acttaaaaag aaagatgata aaacttatga
     4141 atgtagcttc tataatcccg aatctacaaa taccgcttca gatattgaat ctaaaatttc
     4201 aatgactgga tactctatta caaacaaaaa tacgtcgact tctaatgaat ccggcgtgcg
     4261 ctttgaatta caacaaacat taaataaaga tgataattaa tgatttaaaa ttatagtaac
     4321 atgatggttt attat
//
LOCUS       DAMZKC010000180         4309 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.182, whole genome shotgun sequence.
ACCESSION   DAMZKC010000180 DAMZKC010000000
VERSION     DAMZKC010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4309
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.182"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(171..2780)
                     /gene="adhE"
                     /locus_tag="POB55_002315"
                     /gene_synonym="adhC"
     CDS             complement(171..2780)
                     /gene="adhE"
                     /locus_tag="POB55_002315"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDI6501259.1"
                     /translation="MLTIPEKENRGSKEQEVAIMIDALADKGKKALEALSKKSQEEID
                     HIVHQMSLAAVDQHMVLAKLAHEETGRGIYEDKAIKNLYASEYIWNSIKDNKTVGIIG
                     EDKEKGLTYVAEPIGVICGVTPTTNPTSTTIFKAMIAIKTGNPIIFAFHPSAQESSKR
                     AAEVVLEAAMKAGAPKDIIQWIEVPSIEATKQLMNHKGIALVLATGGSGMVKSAYSTG
                     KPALGVGPGNVPSYIEKTAHIKRAVNDIIGSKTFDNGMICASEQVVVIDKEIYKDVTN
                     EFKAHQAYFVKKDELQRLENAIMNEQKTGIKPDIVGKSAVEIAELAGIPVPENTKLII
                     AEISGVGSDYPLSREKLSPVLALVKAQSTKQAFQICEDTLHFGGLGHTAVIHTEDETL
                     QKDFGLRMKACRVLVNTPSAVGGIGDMYNELIPSLTLGCGSYGRNSISHNVSATDLLN
                     IKTIAKRRNNTQIFKVPAQIYFEENAIMSLTTMDKIEKVMIVCDPGMVEFGYTKTVEN
                     VLRQRTEQPQIKIFSEVEPNPSTNTVYKGLEMMVDFQPDTIIALGGGSAMDAAKAMWM
                     FFEHPETSFFGAKQKFLDIGKRTYKIGMPENATFICIPTTSGTGSEVTPFAVITDSET
                     NVKYPLADFALTPDVAIIDPQFVMSVPKSVTADTGMDVLTHAMESYVSVMASDYTRGL
                     SLQAIKLTFEYLKSSVEKGDKVSREKMHNASTLAGMAFANAFLGIAHSIAHKIGGEYG
                     IPHGRANAILLPHIIRYNAKDPQKHALFPKYEFFRADTDYADIAKFLGLKGNTTEALV
                     ESLAKAVYELGQSVGIEMNLKSQGVSEEELNESIDRMAELAFEDQCTTANPKEALISE
                     IKDIIQTSYDYKQ"
     gene            complement(3230..3714)
                     /gene="tnpA"
                     /locus_tag="POB55_002316"
                     /pseudo
     CDS             complement(3230..3714)
                     /gene="tnpA"
                     /locus_tag="POB55_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500412.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(4103..4291)
                     /locus_tag="POB55_002317"
     CDS             complement(4103..4291)
                     /locus_tag="POB55_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001822031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501260.1"
                     /translation="MKRDFFEKWSFILNLILFCAFLFLVVLMFYKDVDLIYIFCTLLI
                     SLLPLLNIIKRYKKKTDN"
BASE COUNT         1264 a          828 c          631 g         1586 t
ORIGIN      
        1 caatcttaat attttcttta tttaaaaaaa tgattgtcta gtttgtatct ctctgaagat
       61 ttggcaataa ataaaagccg ataaccgtat aatgattatc gacttaaagt ttatgtggca
      121 ttttttactt ttgtaatttc aggtgagtta gatgattatt atcagataga ttattgctta
      181 taatcatatg atgtttgaat gatatctttg atttcactga ttagtgcttc tttaggatta
      241 gcagttgtac attgatcttc aaatgcgagc tctgccattc tatcaattga ctcatttaat
      301 tcttcttcag acacaccttg tgatttcaaa ttcatttcaa ttccgactga ttgacctaat
      361 tcgtagacag ctttagctaa tgattctaca agtgcttctg tcgtattacc ttttaatcct
      421 aagaatttgg caatatctgc ataatctgta tctgctctga agaactcata tttagggaat
      481 aacgcatgtt tttgcgggtc tttggcatta taacggataa tatgcggtag taatatcgca
      541 ttcgctctac catgcggaat accatattcg ccaccaattt tatgcgcaat tgagtgtgca
      601 atgcctaaga atgcatttgc aaatgccata ccagccaaag ttgatgcgtt atgcattttc
      661 tctcttgaaa ctttatcacc cttttcaaca gatgatttta aatattcgaa cgtcaattta
      721 atcgcttgta gactcaaacc tcttgtgtag tctgaagcca ttacagatac atatgattcc
      781 attgcatgcg ttagtacatc cattcctgta tctgctgtaa cgctttttgg cacactcatc
      841 acaaattgag ggtcaataat tgcaacgtca ggtgttaaag caaaatcagc caacggatat
      901 tttacatttg tttcactatc tgtgataact gcaaatggtg ttacttctga acctgtacct
      961 gatgtcgtag ggatacaaat gaacgtcgca ttttcaggca tgcctatttt ataagtacgt
     1021 ttaccgatgt ctaggaactt ttgtttagca ccgaagaatg atgtctcagg gtgttcaaag
     1081 aacatccaca ttgcttttgc agcatccatc gctgaaccac caccaagtgc aatgattgta
     1141 tccggttgga aatcaaccat catttccaga cctttatata ctgtattagt tgatgggttc
     1201 ggttcgactt cgctaaatat tttaatttga ggctgttccg ttctttgtct taatacattc
     1261 tcaactgttt ttgtataacc gaattctacc ataccagggt cacagacaat catcactttt
     1321 tcaatcttgt ccattgttgt tagactcatg attgcatttt cttcaaaata aatttgagca
     1381 ggcaccttga aaatttgagt attattacgt cgtttagcaa tcgttttaat gtttaataaa
     1441 tctgtcgcac taacattatg tgaaattgag tttctaccgt acgaaccaca acctaatgtt
     1501 aaagacggaa tcaattcgtt atacatatca ccaatacctc caaccgctga tggtgtattt
     1561 acaagtacac gacaagcttt cattcttagt ccaaaatctt tttgtaatgt ttcatcttct
     1621 gtatggataa cggctgtgtg tcctaatcca ccaaaatgta gtgtgtcttc acaaatttga
     1681 aatgcttgtt ttgtagattg ggcttttact aaggctaata ctggagataa tttttcacga
     1741 gataacggat agtctgaacc tacaccgcta atttcggcta tgataagttt tgtattttcg
     1801 gggacaggta tacctgctaa ttcagctatt tcaactgcag atttaccgac aatatcaggc
     1861 ttaatacctg ttttttgttc attcataatt gcattttcta agcgttgtaa ttcatctttt
     1921 ttaacaaagt atgcttgatg tgctttaaat tcattagtaa catctttata aatttcttta
     1981 tcaatgacta caacttgttc agaagcacaa atcataccat tatcaaatgt ttttgaacca
     2041 atgatatcat ttactgcacg tttaatgtgt gctgtttttt caatgtaaga cggcacgtta
     2101 cctggtccca cacctaatgc cggtttgcca gttgaatatg cagacttaac catgcccgaa
     2161 ccacctgttg ctagaactaa tgcaatacct ttgtgattca ttaattgttt tgttgcttcg
     2221 atagaaggca cttcaatcca ctgaataata tctttaggtg cacctgcctt cattgccgct
     2281 tctaatacaa cttctgctgc acgcttcgac gattcttgtg cacttggatg gaatgcaaaa
     2341 atgattggat ttcctgtctt aattgcaatc atcgctttaa aaatagttgt cgacgtagga
     2401 tttgttgttg gcgtaacacc acaaataaca ccaattggtt ccgctacata cgttaatcct
     2461 ttttctttat cttcaccaat aatccctact gtcttattgt cttttattga attccatata
     2521 tattcagaag cgtataaatt tttaatcgct ttatcttcgt atatacctct tccagtttct
     2581 tcatgtgcta attttgctag caccatatgt tgatcaacag ctgctaagct catttgatga
     2641 acaatatgat caatttcttc ttgtgacttt ttagataatg cttctaatgc ttttttccct
     2701 ttgtcagcta gagcatcaat cataattgcc acttcttgtt ctttcgatcc acgattttct
     2761 ttttcaggta tagttaacat atacaaccac tcctttatac tttgtgaatt atttcacaaa
     2821 cattatagta catgtctctc aggatataaa gaaaattcta tgcaaaaaag tttaatttcg
     2881 aatattattt gaacaaatat caaattttaa aataaatgtt ttcatgaaat cattgttatt
     2941 tcggtgtttt tagaatgatt ttataatcat aattttttca atgacataat ttattcataa
     3001 ttatatattt aattctgaat attctttaat agcctaaaaa gcttgttata tcgcacaaaa
     3061 cacaacatta aaaggtcctt gtcgatacct atcaacagat gttacaaata aaaaccaccc
     3121 gtgtgaacgg gtggtttgtt ctgcggctat aagccttcct tactggccag ccctaaaagg
     3181 gcactgacaa gtcagccaac tgcactacta ttccagcaat cctaaaggtt tactcttttt
     3241 tctttctttt tttatttttc tctccagtga aaggatctaa atattcttcc attgagattt
     3301 catctgcaac gatatcctct tgtaattgat tacgaatata attttcaatc acttttttat
     3361 ttctacctac tgtatccaca taaaatcctt tacaccaaaa ctttctattt ccatatctat
     3421 actttaagtt agcatgtcta tcaaatatca ttaaattact tttcctttta aatagccaac
     3481 aaatgatgat accccaagtt tgggtggtat acttactaac atatggatat gatctttaca
     3541 tacctctgct tcaattatct ctacaccttt tctttcatat aattgacgta atataatccc
     3601 tatatctttt tttatttttc catatatcgc ttgtcttctg tatttaggtg caaagacaat
     3661 atgttactta caattccatt tcgtatgtgc taaactgttt gtgtcagatg acattaaata
     3721 gcatctcctc gtgttgatta ttttggttgg ctgaccaata tttactctaa catgtagaga
     3781 tgcatttttt tgacaatggt agaacctttt ctggggagtg ggacagaaat gatattttcg
     3841 caaaatttat ttcgccgtcc caccccaact tgcattgtct gtagaaattg ggaatccaat
     3901 ttctctttgt tggggccccg ccccaactcg cattgcctgt agaatttctt ttcgaaattc
     3961 tctgtgttgg ggcccctgac tagaattgaa aaaagcttat tacaagcgca ttttcgttca
     4021 gtcaattact gccaatataa cttcgtagat catagaagat tgatttattt cccagcctat
     4081 tcttttcata aaaaaagacg gattaattat ccgtcttttt cttatatctt tttataatat
     4141 ttagcaaagg taataatgat attaataatg tacaaaaaat ataaatcaaa tcgacatcct
     4201 tataaaacat cagaaccact aaaaacaaaa aagcacaaaa taaaattaaa tttaaaataa
     4261 acgaccactt ttcaaaaaaa tctcttttca tatttccacc cctaatttt
//
LOCUS       DAMZKC010000181         4195 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.183, whole genome shotgun sequence.
ACCESSION   DAMZKC010000181 DAMZKC010000000
VERSION     DAMZKC010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4195
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.183"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            122..664
                     /locus_tag="POB55_002318"
     CDS             122..664
                     /locus_tag="POB55_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'(3')-deoxyribonucleotidase"
                     /protein_id="HDI6501261.1"
                     /translation="MTRKSIAIDMDEVLADTLGEIIDAVNFRADLGIKMEALNGQKLK
                     HVIPEHDGLITEVLREPGFFRHLKVMPYAQEVVKKLTEHYDVYIATAAMDVPTSFSDK
                     YEWLLEFFPFLDPQHFVFCGRKNIVKADYLIDDNPRQLEIFTGTPIMFTAVHNINDDR
                     FERVNSWKDVEQYFLDNIEK"
     gene            complement(1345..2262)
                     /locus_tag="POB55_002319"
     CDS             complement(1345..2262)
                     /locus_tag="POB55_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HDI6501262.1"
                     /translation="MENKYTHGVLFYHEHSGLKNINQGIGEVTTALSSICKHLSIQLS
                     ENEGDIIKYCQEIKTKNYAKDVDILFILGGDGTVNELINGVMTHDLQLPIGILPGGTF
                     NDFTKTLNIAPNHKQASEQMISAQVGKYDVIKINNQYALNFVGLGLIVQNAENVQDGS
                     KDIFGKLSYIGSTVKTLLNPTQFNYQLSIDDKTYSGETTMILTANGPFIGGSRIPLTD
                     LSPQDGELNTFIFNEQSFSILNDIFKKRDSMNWNEITQGIEHIPGKKISLTTDPAMKV
                     DIDGEISLETPIDIEVIPNAIQLLTVNDL"
     gene            2352..2453
                     /locus_tag="POB55_002320"
     CDS             2352..2453
                     /locus_tag="POB55_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501263.1"
                     /translation="MVITDIDVYRVGRENIVIAVYNEHIKLLFILIS"
     gene            complement(2531..4036)
                     /locus_tag="POB55_002321"
     CDS             complement(2531..4036)
                     /locus_tag="POB55_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HDI6501264.1"
                     /translation="MTQQNSHGNQIQDIPQTGFFGHPRGLGVLFFVEFWERFSYYGMR
                     ALLIFYMYFAVTDNGLGIDKTTAMSIMSVYGSLIYMTSIPGGWIADRITGTRGATLLG
                     AVFIIIGHICLSLPFALIGLFTSMFFIIIGSGLMKPNISNIVGRLYPENDRRMDAGFV
                     IFYMSVNMGALLSPIILQHFVNVKNFHGGFLIAAVGMALGLVWYVLFNRKNLGSVGMK
                     PTNPLTPAEKKKYGLIIGSVVLAIVLIIVIGALTNSLSFNLVSNTVLVLGIALPIIYF
                     TLIIRSKDVTDTERSRVKAFIPLFILGMVFWAIQEQGSNVLNIYGIEHSDMKLNLFGW
                     KTNFGEAIFQSINPLFILLLAPIISLLWQKLGTKQPSLPVKFAIGTFLAGASYILIGI
                     VGYASGSSNFSVNWVILSYIICVIGELCLSPTGNSAAVKLAPKAFNAQMMSIWYLTNA
                     SAQAINGTLVKLIEPLGQTNYFIFLGVVAIIVTTIVLAFSPLIIKAMKGIR"
BASE COUNT         1464 a          671 c          689 g         1371 t
ORIGIN      
        1 gcagtgtgaa tgatgaggtg agagtattca gtgtaaaaag caacaataga tgatattgtt
       61 ttgtatcaat tgcttttttg ctatactgaa tcaatactga tattttgagg agaagattaa
      121 aatgacccgt aaatcaatcg cgattgatat ggatgaagta ttggcagata cattaggaga
      181 aatcattgat gctgtcaatt ttagagcgga tttaggtatt aaaatggaag ctttgaatgg
      241 tcaaaaactt aaacatgtta ttcctgaaca tgatggatta attacagaag tattgagaga
      301 accaggcttc ttcagacatc ttaaagtgat gccgtatgca caagaagttg tgaaaaaatt
      361 aactgaacat tatgatgtat atattgctac agcagcaatg gatgtaccaa catcatttag
      421 tgataaatat gaatggttac tagagttctt tccattttta gatcctcagc attttgtttt
      481 ttgtggtaga aaaaacatcg ttaaagctga ttatttaata gatgacaatc ctagacagct
      541 tgaaattttt actggtacac cgattatgtt tacagcagtg cataatatta atgatgatcg
      601 atttgaacgc gtaaatagct ggaaagatgt agaacagtat tttttagata atattgagaa
      661 ataaaaatat atcacttgaa aaatttcatg tagtaaagat gatggatagg ctataaagta
      721 attgtgactt ggttgaactt ttatgtctta gacactacaa cactatattg gcagtagttg
      781 actgcggggc cccaacataa agaaattgga acgccaattt ctacagacaa tgcaagttgg
      841 cggggcccca acatagagaa attggaacgc caatttctac agacaatgca agttggggtg
      901 ggccccaaca taaagaaata ctttttcttt agaaattagt atttcttatg catgagtgta
      961 actcatgcat tcatattttt aagtacacat tagctgtgac taatgataaa gaatcgctac
     1021 ataatcaatc attagtcgtt ctttatcatt tccgtcccgc tctcaataaa tgttagtcta
     1081 tcttattatt ataaatcgga tgaatgtgtt aatctatggc agattacacg tcatccgatt
     1141 ttttatagaa tttgaaaaag acgcataaac cactatgatt taaaatacaa catcaatcat
     1201 tttagtggca tgcgccaaaa ttatatgtct gtttttgaaa cagggtaata gcttaaagct
     1261 aataaaaacg aatataaggt gcgttgaatc ttatgattac actccaaacc taatataata
     1321 tcgggttaag atcattccgg atgcttacaa atcattgaca gtaagtaact gaatggcatt
     1381 tggtataacc tcaatatcaa taggtgtttc taatgaaatt tcgccatcaa tatcaacttt
     1441 cattgctgga tctgttgtaa gtgaaatctt tttaccaggt atatgctcaa taccttgagt
     1501 aatttcattc caattcatgc tatcacgctt tttaaaaata tcatttaaaa tactgaaact
     1561 ttgttcatta aaaatgaaag tgttcagttc accatcttga ggagacaaat cagtcaatgg
     1621 tatacgacta ccaccaatga atggaccatt tgctgttagt atcatggtcg tttcgccaga
     1681 atatgtctta tcatctattg ataattgata attaaattgt gttggattta gcagtgtttt
     1741 gacagttgat ccaatataac tcaatttacc aaatatatct tttgaaccat cttgtacgtt
     1801 ttcagcgttt tgaacaatga gacctaagcc aacaaagttg agtgcatatt gattatttat
     1861 tttaattaca tcgtatttac caacttgtgc agaaatcatt tgttcactag cttgtttatg
     1921 attaggtgct atatttagcg tttttgtaaa atcattaaaa gtaccgcctg gtaaaatgcc
     1981 aatagggagt tgaaggtcat gtgtcataac accgtttata agttcgttaa ccgtgccatc
     2041 accgccaaga ataaataata tatctacatc ttttgcatag tttttagttt tgatttcttg
     2101 gcaatattta ataatgtcac cttcgttttc actcaattga atagaaagat gcttacaaat
     2161 tgaacttaat gctgttgtaa cttccccaat accttgatta atatttttta atccactgtg
     2221 ttcatggtaa aagaggacac catgtgtata tttattttcc atagtttagc ctactttcta
     2281 aaaattggtt cattaaatat atatacccac ttttaattgt taataccaaa aatatgtttt
     2341 aaatagagaa aatggtaata actgacattg atgtctatag agtgggacga gaaaatatag
     2401 ttatagctgt ctataacgag catattaagc ttttatttat actgatatct tgaatttaat
     2461 taatagaaac ctataaaaaa acagtaagcc atttaaatga cttactgttt tttgaattag
     2521 gccaacaata ttaacgtata cctttcatcg ctttgatgat taaaggtgag aatgctaata
     2581 caattgttgt aacaataatt gcaacaacac ctaggaaaat aaagtaattt gtttgaccta
     2641 gtggttctat taacttaact aaagtaccat tgattgcttg tgcagaagcg ttagttaagt
     2701 accaaatact catcatttgg gcattaaatg ctttaggtgc taacttaaca gcagcactat
     2761 tacccgttgg tgataagcat agctcaccga taacacaaat aatgtacgat aaaataaccc
     2821 agttaactga aaagtttgat gaacctgatg cataacctac aataccaatt agtatgtatg
     2881 acgcacctgc taagaacgta ccaattgcaa attttactgg caggctaggt tgtttagttc
     2941 caagcttttg ccataaaagt gaaataattg gagctagtaa taaaataaat aatgggttaa
     3001 ttgattggaa gatcgcttca ccaaagtttg ttttccaacc aaataagttt aatttcatat
     3061 ctgaatgttc aattccatat atgtttaata cattagaccc ttgttcttga atagcccaga
     3121 acaccattcc aagaataaat aatggaataa atgctttaac acgagaacgt tcagtatcag
     3181 tgacatcttt acttctaata attaaagtga agtaaatgat tggtaatgca atacctaata
     3241 ctaaaacagt attactaact aagttaaatg ataatgagtt agttaatgca ccaataacga
     3301 taattaatac aattgctaaa acaacacttc cgataataag accatacttt ttcttttcag
     3361 ctggtgtcaa tgggttagta ggtttcatac caacgctacc taagtttttg cggttgaaaa
     3421 gtacatacca tactaaacct aatgccatac caactgctgc aatcaagaat ccgccgtgga
     3481 agtttttaac attaacaaag tgttgcaaaa taataggtga taataatgca cccatattaa
     3541 ctgacatata gaaaataaca aaacctgcat ccatacgtct atcattttca ggatataaac
     3601 ggccaacgat atttgaaatg tttggcttca ttaaacctga accaataatg atgaagaaca
     3661 ttgatgtgaa taagccgatt aatgcaaatg gtaagcttaa acaaatatgt ccgataataa
     3721 taaagactgc acctaataaa gtagcgcctc tagtacctgt aattctgtca gcaatccatc
     3781 cgcctggtat tgatgtcata tagattaatg aaccataaac tgacataatt gacatagctg
     3841 ttgttttatc aattccaagg ccattatctg ttacggcaaa gtacatgtag aaaatgagta
     3901 gggcacgcat gccataataa ctaaaccttt cccagaactc tacaaagaag agtacgccta
     3961 gtcctcgagg atgcccgaaa aatcctgttt gaggtatgtc ttgaatttga tttccatggg
     4021 agttttgttg tgtcatgtat acatcccatc ctttcttccc ctaatacaat agtttaagta
     4081 aatttgattg tgccttatca actaaatatt ccaacaaatc acaaaaaaat aaaaatattg
     4141 agaatattct gttattaagc gcgcacaatt aattagagat aatataaaaa tatac
//
LOCUS       DAMZKC010000182         4165 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.185, whole genome shotgun sequence.
ACCESSION   DAMZKC010000182 DAMZKC010000000
VERSION     DAMZKC010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4165
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.185"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            16..759
                     /locus_tag="POB55_002322"
     CDS             16..759
                     /locus_tag="POB55_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDI6501265.1"
                     /translation="MLETKDLNLFLGNKHVLKNISLSIPVRGEIIGIMGPNGAGKSSL
                     IKSLIGEFNATGTKLLYNKPIQQQLQHITYIPQKAHIDLDFPISVEQVILSGCYKEIG
                     WFRRPNKSARDKLKQLLSDLELESLRHRQISELSGGQLQRVLVARALMSESEVYFLDE
                     PFVGIDFSSEKLIMTKIENLKQQGKLILIIHHDLSKAKQYFDRIILLNQTLRYFGDSE
                     EAMSVTRLNETFMSSTDCSDPSQRSNITC"
     gene            753..1589
                     /locus_tag="POB55_002323"
     CDS             753..1589
                     /locus_tag="POB55_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDI6501266.1"
                     /translation="MLEFVEHLFTYQFLNRALITSIIVGIVCGTVGSLIVLRGLSLMG
                     DAMSHAVLPGVALSFLFGIPMFVGALITGMIASIFIGYITSSSKTKPDAAIGISFTAF
                     LASGIIIISLINTTTDLYHILFGNLLAITNSAFLTTIVIGSIVLILIIIFYRPLMIST
                     FDPTFSRMSGLNTTLLHYFVMLLLSLVTVASIQTVGIILVVALLITPASTAFLISKKL
                     YSMMIIASLISVISSIVGLYYSYIYNIPSGATIVLCTFVIYIITLFFTKFTNRKKRGS
                     LT"
     gene            1586..2515
                     /locus_tag="POB55_002324"
     CDS             1586..2515
                     /locus_tag="POB55_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HDI6501267.1"
                     /translation="MKKLVPLLLALLLLVAACGTGGKQSSDKSNGKLKVVTTNSILYD
                     MAKNVGGDNVDIHSIVPVGQDPHEYEVKPKDIKKLTDADVILYNGLNLETGNGWFEKA
                     LEQAGKSLKDKKVIAVSKDVKPIYLNGEEGNKDKQDPHAWLSLDNGIKYVKTIQQTFI
                     DNDKKHKADYEKQGNKYIAQLEKLNNDSKDKFNDIPKEQRAMITSEGAFKYFSKQYGI
                     TPGYIWEINTEKQGTPEQMRQAIEFVKKHKLKHLLVETSVDKKAMESLSEETKKDIFG
                     EVYTDSIGKEGTKGDSYYKMMKSNIETVHGSMK"
     gene            complement(3543..>4165)
                     /locus_tag="POB55_002325"
     CDS             complement(3543..>4165)
                     /locus_tag="POB55_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HDI6501268.1"
                     /translation="HDKLAFLKTVEKDDENSKELERLQKIAFNVETKTLESLVDEGQI
                     TNSVLENYMRYAERTQVYRQASLIRRMIVLLRGALLKRRVQTRVNSASSLSVTDNLME
                     LNKINKLVHYNVVSRLSKETTKDNTLEVGMVCDGYLMRIENLTPSNFFNSASEDTITK
                     IKLNALREQRRILRELIDTDEVSEGTALKLREAINYDEMVIVDSMT"
BASE COUNT         1426 a          686 c          662 g         1391 t
ORIGIN      
        1 aggaggtata aacgtttgtt agaaacaaaa gatttaaatc tgtttttagg taataagcat
       61 gtacttaaaa acatttcctt atcgatacca gtacgcggcg aaataattgg tatcatgggc
      121 ccgaatggtg ctggtaaatc ttcccttatc aagtctttaa ttggtgaatt taatgctacc
      181 ggtactaaat tgttatataa caaacctata caacaacaac tgcaacatat tacatatatt
      241 ccacaaaaag cacatattga tttagatttt cctataagtg tggaacaagt gattttatca
      301 ggttgctaca aagaaattgg atggtttaga cgacctaata aatcagcaag ggataaactc
      361 aaacagttat taagcgattt agaattagaa tctttacgtc atcgacaaat ttcagaatta
      421 agtggtggac aattacaacg tgtgctagta gcaagagcat tgatgtccga aagtgaagtt
      481 tattttctag atgagccgtt tgtcggaatt gattttagta gcgaaaaatt aatcatgaca
      541 aaaatcgaga acttaaaaca acaaggaaaa cttattctta tcatccacca tgatctatca
      601 aaagcaaagc aatactttga tcgcattatt ctattaaatc aaacattacg atactttggt
      661 gatagtgaag aggctatgag tgtcactcgc ttaaacgaaa catttatgag tagcactgac
      721 tgtagtgacc ctagtcaaag gagcaatata acatgttaga gtttgtcgaa catttattta
      781 catatcaatt cttgaatcga gcattgataa cttcaattat tgtagggata gtttgtggta
      841 cagttggtag tttaattgta ttacgtggtc tttcattaat gggagatgca atgagtcacg
      901 cagtattacc tggtgttgcc ctatcattct tatttggtat tccaatgttt gtaggcgcac
      961 ttataactgg tatgatcgca agtattttta tcggttatat cacatctagt agtaaaacga
     1021 aacctgacgc cgctattgga attagtttta ccgctttctt agcttcgggg attattatta
     1081 ttagcttaat aaacactacg acagatttgt accatattct gtttggtaat ttactggcaa
     1141 tcacaaatag tgcattttta acaactattg tgattggttc aatcgtcctt attctaatca
     1201 ttattttcta tcgtccatta atgatttcta cattcgatcc aacgtttagt agaatgagtg
     1261 gtctaaatac gacgttatta cattactttg tgatgttgtt actctcatta gtaacagtag
     1321 caagtattca aacggtaggt attattcttg tagttgcatt attaattaca cctgcatcta
     1381 cagcattttt aattagtaaa aaactatact caatgatgat tatagcaagt ttaataagtg
     1441 tcataagttc aattgtcgga ctttattaca gctatattta caacattcct agtggtgcga
     1501 caatcgtact ttgtacgttt gtaatttata tcatcacatt atttttcaca aaatttacga
     1561 atagaaagaa acgaggaagt ttaacatgaa aaaattagta cctttattat tagccttatt
     1621 acttctagtt gctgcatgtg gtactggtgg taaacaaagc agtgataagt caaatggcaa
     1681 attaaaagta gtaacgacga attcaatttt atatgatatg gctaaaaatg ttggtggaga
     1741 caacgtcgat attcatagta ttgtacctgt tggtcaagat cctcatgaat atgaagttaa
     1801 acctaaagat attaaaaagt taactgacgc tgacgttatt ttatacaacg gattaaattt
     1861 agagactggt aacggttggt ttgaaaaagc cttagaacag gctggtaaat cattaaaaga
     1921 taaaaaagtt atcgcagtat caaaagatgt taaacctatc tatttaaacg gtgaagaagg
     1981 caacaaagat aaacaagatc cacacgcatg gttaagttta gataacggta ttaaatacgt
     2041 aaaaacaatt caacaaacat ttatcgataa cgacaaaaaa cataaagcag attatgaaaa
     2101 gcaaggtaac aaatacattg ctcaattgga aaaattaaat aatgacagta aagacaaatt
     2161 taatgacatt ccaaaagaac aacgtgccat gattacaagt gaaggtgcct tcaagtactt
     2221 ctcaaaacaa tacggtatta caccaggtta tatttgggaa attaacactg aaaaacaagg
     2281 tacacctgaa caaatgagac aagctattga gtttgttaaa aagcacaaat taaaacactt
     2341 attagtagaa acaagtgttg ataagaaagc aatggaaagt ttatctgaag aaacgaagaa
     2401 agatatcttt ggtgaagtgt acacagattc aatcggtaaa gaaggcacta aaggtgactc
     2461 ttactacaaa atgatgaaat caaatattga aactgtacac ggaagcatga aataacacgc
     2521 tgtgttttaa tgaagtaaga tgaattgatg ttgatgcaac ctaaaatatt ggtatctcca
     2581 atattttagg ccacacatca acataacaaa gtcgaaggct aatagtccca tatcgtgcgt
     2641 taaatatata ttaccctcct attaatatat ataccgttcc cgatcgcacg atatggtggt
     2701 attagaactt ctctttgaac gaaagagaaa agctagaagt tcttatgcag ttttaattaa
     2761 actgtaaaca tttgtcactc tttaaatcaa agagtaaagt taaaagcttt atgtggtttt
     2821 gattaaactg cgaacagctg cttctctttg aacgagagag aaaagctaga agttcttatg
     2881 cagttttaat taaactgtcg ttcccttcat ctcttttaac cacagagatg cgttagaagt
     2941 tcttctaata caatttatac aacgccattc cctacacact cttataaaag agattcacgc
     3001 gcgtcaataa attgtattac atactaacta aaaagctttt cttaatcgta ctaacgaagt
     3061 tagaggttct tatgtgattg gacaactgtt ctccctacta gacttttagc atagctggtt
     3121 aaaagtacta ttacagattg ttcaattgta tgcccctttc ctttgaacgg aatattaaaa
     3181 gctatgtgca gtaactttta ataggcatac cacttttgca tcaagctata aataatcgca
     3241 tactttgata tttaatctgg cctatcaaag taaacatcta tggtttcctc ccccccatag
     3301 atccttaagc cacactcatc cgatgtcata tgagtgtggc tattttttgt gaatacactt
     3361 aacttcacct ttaaatgact tcgcttaatc tatctcctcc aatttttaaa acttatttca
     3421 attaaatctc aaatattaat aattcgtata gtttaaaact atctattcaa ttcccataaa
     3481 ataaaaggga ttgatgaaaa gcccttcagt ttttcatcaa tcccttttag cataattagg
     3541 aactacgtca tactatctac aataaccatt tcatcgtaat tgatggcttc tcttagtttt
     3601 aacgctgtac cttctgatac ttcatctgta tcaatcaact cacgtaaaat gcgacgttgt
     3661 tctctcaatg catttaattt aattttagta atcgtatctt cacttgctga gttgaagaaa
     3721 tttgatggtg ttaagttttc aattcgcatt aaataaccgt cacaaaccat tccaacttca
     3781 agtgtattat cttttgttgt ttccttagac aaacgactaa ccacattata atggactaat
     3841 ttattaattt tatttaattc cattaagtta tccgtaacac taagtgaaga tgcggagttc
     3901 actctcgttt gtactcttcg ttttaataaa gcacctcgta ataatacaat cattcttctt
     3961 attaatgatg cttgtctata tacctgtgtt ctttcagcat aacgcatata gttttcaagt
     4021 acactattcg ttatttgtcc ttcatctact aaagactcta atgtttttgt ttctacatta
     4081 aaagcaattt tttgtagacg ttctaattct ttagagtttt catcatcttt ctctacagtt
     4141 tttaaaaatg ctaatttatc atgat
//
LOCUS       DAMZKC010000183         4137 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.186, whole genome shotgun sequence.
ACCESSION   DAMZKC010000183 DAMZKC010000000
VERSION     DAMZKC010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4137
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.186"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(261..2069)
                     /gene="pepF"
                     /locus_tag="POB55_002326"
     CDS             complement(261..2069)
                     /gene="pepF"
                     /locus_tag="POB55_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HDI6501269.1"
                     /translation="MSQQLSREEQERKYPEYTWDLTTIFKDDEAFEAAFKEVENELGK
                     EEQFKGHIGDSAETLYNALELEDTLGTKLEKVYVYAHLKQDQDTTNDKYTGMESRAHQ
                     LIIKFSSAWSFLVPEILQIDEDKIQSFVNSYDKLQKFAFDLKLINEKRPHILDAETEK
                     LLTEAQDALSTPSNVYGMFSNADLVFEDAIDKDGNAHPLTQGTFIKYLESDDRKLRES
                     AFRNVYKAYGAHNNTLGATLAGEVKKNVFNARTHNYKTAREKALSNNHIPENVYDNLV
                     KTVHKYLPLLHRYTELRKELLGLDDLKMYDLYTPLIKDIKFEMPYEEAKEWMLKALEP
                     MGEEYLNVVKEGLNNRWVDVYENKGKRSGGYSSGAHLTNPFILLNWSNTISDLYTLVH
                     EFGHSAHSYFSRKFQPSNSSDYTIFVAEVASTCNEALLSDYMDKHLNDEKRLLLLNQE
                     LERFRATLFRQTMFAEFEHKIHAIEEAGEPLTPTRMNEEYAKLNKLYFGDSVETDEDI
                     SKEWSRIPHFYMNYYVYQYATGYSAAQSLSHQILTEGKPAVDRYINEFLKKGSSNYPI
                     EILKNAGVDMTTPEPIEQACEVFEQKLNAFEKLMKA"
     gene            complement(2117..3103)
                     /locus_tag="POB55_002327"
     CDS             complement(2117..3103)
                     /locus_tag="POB55_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HDI6501270.1"
                     /translation="MLVALNEEKERVLATTALRKTQYFCPVCGKQVILKRGLKVISHF
                     AHKHLAEQKCFNNETIKHYKSKLILAQMIQQQGFKVEIEPFLKEIKQIPDILINNKYV
                     IELQYSPIPYKQILQRTEGLKKMGYKVSWLLNDVDYCHNKVKFNHFQSMFINPFTRKL
                     HTFNLEKKQIMMFQQIQYLGGHKYVAEKRNAKISELFNEAPCDYHAVYKLSKFAINQY
                     IKYCRWQNSVLEPTLSAMYQLQLTDQEVVHNYGYIFPEQIYIKNHPIEWQLQVDLWLK
                     NGKSKLVNDNLNYFKLKKFIVGLESKTAIIEKLINNYLNICSDRGNDVQILF"
     gene            complement(3224..3943)
                     /gene="mecA"
                     /locus_tag="POB55_002328"
     CDS             complement(3224..3943)
                     /gene="mecA"
                     /locus_tag="POB55_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HDI6501271.1"
                     /translation="MRIERVDDTTVKLFITYSDIEARGFSREDLWTNRKRGEEFFWSM
                     MDEINEEEDFVVEGPLWIQVHAFEKGVEVTISKSKNEDMMNMSDDDATDQFDEQVQEL
                     LAQTLEGEDQLEELFEQRTKEKEAQGSKRQKSSARKNTRTIIVKFNDLEDVINYAYHS
                     NPITTEFEDLLYMVDGTYYYAVHFDSHVDQEVINDSYSQLLEFAYPTDRTEVYLNDYA
                     KIIMSHNVTAQVRRYFPETTE"
BASE COUNT         1295 a          726 c          525 g         1591 t
ORIGIN      
        1 aaaagtgcat atttaataca tttttataca acttcacttc cctcttatta aaaaagaaca
       61 taaaaaaacg tcgtgtctga atacacgacg ctaaacaaag gggatgggag aaatttttca
      121 cttcaaacaa aggggtatgt ttgttatgtg attaatttca tgttcataat ataacatgct
      181 atctctacca tttcaacctt ttaatagtac ttaatttaaa ttgtcacaat ctgttcataa
      241 tgtaagcgaa tacaatagta ctaagctttc attaattttt caaaagcgtt caatttttgt
      301 tcaaaaactt cacaagcttg ttcaattggt tcaggtgttg tcatatctac accagcattt
      361 tttaatatct caattggata atttgagcta ccttttttca agaattcatt aatatatcta
      421 tctactgctg gcttaccttc tgttaaaatt tgatgactta agctttgagc tgcactgtaa
      481 ccagttgcgt attgatatac ataataattc atatagaagt gtggaatacg tgaccattcc
      541 ttactaatat cttcatcagt ttctacagaa tcaccgaagt ataatttatt taatttggca
      601 tattcttcat tcattctagt tggtgttaaa ggttcacctg cttcttcaat tgcatgaatt
      661 ttatgctcaa attctgcgaa cattgtttgt cggaataatg tagctctgaa acgttctaat
      721 tcttggttta ataataataa gcgtttttca tcattaagat gtttatccat ataatcactt
      781 aaaagtgctt cgttacaagt tgatgcaact tcagcgacaa aaatagtgta gtcactagaa
      841 tttgacggtt ggaattttct actgaagtaa ctatgtgctg aatgcccaaa ttcatgaact
      901 aatgtgtata agtctgaaat agtattagac cagttaagta gaataaatgg attagttaaa
      961 tgtgcacctg atgaatagcc acctgaacgt ttacctttat tctcatagac atcgacccaa
     1021 cgattgttta agccttcttt aactacattt aaatattctt cacccattgg ttctaaagct
     1081 tttaacatcc actctttagc ttcttcataa ggcatttcaa acttaatatc tttaattaat
     1141 ggtgtatata aatcatacat tttcaagtca tctaaaccta gcaattcttt gcgcaattca
     1201 gtatatctat gtagcaatgg taaatattta tgtacagttt ttactagatt gtcatataca
     1261 ttttctggaa tatgattatt actcaatgct ttttctcttg cagttttgta attgtgtgta
     1321 cgagcattaa atacattttt cttcacttca cctgctagcg tagcgccaag cgtattatta
     1381 tgagcaccat atgctttata tacatttcta aaagcacttt ctcttagttt gcgatcatct
     1441 gattctaaat acttaataaa tgtaccttgt gttaacgggt gtgcatttcc atctttatca
     1501 atcgcatctt caaatactaa atcagcgttg ctaaacatac cgtatacatt tgatggcgtt
     1561 gataacgcgt cctgcgcttc tgttaataac ttttcagttt cagcatctaa aatatgagga
     1621 cgtttttcat taatcaactt caaatcaaat gcgaattttt gtaatttatc atatgaattt
     1681 acaaatgatt gaattttatc ttcatcaatt tgtaaaatct ctggcactaa gaaactccat
     1741 gccgagctaa atttaataat taattgatgt gctcttgact ccataccagt atacttgtcg
     1801 ttcgttgtat cttggtcttg ttttaggtgc gcgtatacat atactttttc taatttagta
     1861 cctaatgtat cttctaattc taatgcattg tataatgtct cagcactatc accaatgtgt
     1921 cctttaaatt gttcttcttt gcctaactca ttttcaactt ctttaaatgc agcctcaaaa
     1981 gcttcatcat ctttgaaaat tgttgttaag tcccatgtat attcaggata tttacgttcc
     2041 tgttcttctc tagataattg ttgactcata tgtagtaaac ctcctaataa aatataattg
     2101 atataatttt ctcattttag aacaaaattt gcacgtcatt acctctatct gaacaaatat
     2161 ttaaataatt gttaataagt ttttctataa ttgctgtttt actttctaga ccaacaataa
     2221 actttttcag tttaaaataa ttaagattgt catttactaa tttgcttttt ccattcttta
     2281 accataaatc aacttgtaat tgccactcaa taggatgatt tttaatataa atttgctctg
     2341 gaaaaatata accataattg tgcactactt cttgatcagt taactgtaat tgatacattg
     2401 cacttaaagt gggttctaaa acagaatttt gccagcgaca atatttgata tattgattaa
     2461 ttgcgaactt tgataattta taaacagcat gataatcaca aggcgcctca ttaaacaact
     2521 cactaatttt ggcatttctt ttttcagcga catatttgtg cccgcctaaa tattgtattt
     2581 gttgaaacat cattatttgt tttttctcta aattgaacgt atgaagtttt cgagtgaatg
     2641 gattaataaa catactttga aaatgattga acttcacttt attatgacaa taatcaacat
     2701 catttaataa ccaacttact ttatatccca ttttctttaa accttccgtt cgttgaagaa
     2761 tctgtttata aggaattggc gaatactgta gctcaataac atatttatta ttaatcaaaa
     2821 tatccggaat ttgttttatt tcttttaaaa atggctctat ctctacttta aatccttgtt
     2881 gctgtatcat ctgtgctaaa atcaatttac ttttataatg tttaatcgtt tcattattaa
     2941 aacatttttg ttccgctaaa tgtttatgtg caaaatgact aattactttg agcccacgct
     3001 ttaaaataac ttgcttgcca cacaccggac aaaaatattg tgtctttctc aatgcagtag
     3061 ttgctaacac acgttccttt tcttcattta aagctactaa catatcttca cctcaaaata
     3121 ttacacgtaa gtttaactac tattactttt tcagcttttg aaatcaataa aaaaagagac
     3181 tccaaagagt ctcctttcat agtgcgttgc ttaattaata tcgttattca gttgtctctg
     3241 gaaaataacg tcgaacttga gctgttacgt tatgactcat aattatttta gcatagtcat
     3301 ttaaataaac ttctgttctg tctgttggat aagcaaattc aagcaattga ctgtaactat
     3361 cattaatgac ttcttgatca acatgactat caaaatgtac agcataataa taagtaccat
     3421 caaccatata taacaaatct tcaaactctg tagttattgg attgctatga tatgcataat
     3481 taataacatc ttctaaatcg ttaaatttca caataattgt tcttgtattt ttacgtgctg
     3541 aagacttttg acgtttagaa ccttgagctt ctttttcttt tgttcgttgc tcgaataatt
     3601 cttctaattg atcttcacct tctaatgttt gagctaacaa ttcttgaact tgttcatcaa
     3661 attgatcagt tgcatcatca tcagacatat tcatcatatc ttcattttta gatttagaaa
     3721 ttgtgacttc gacacctttt tcaaaggcat gtacttgaat ccataatgga ccttctacaa
     3781 caaaatcttc ttcttcgtta atttcatcca tcattgacca aaagaattct tcgccacgtt
     3841 tgcgatttgt ccataaatct tcacgactaa atccacgggc ctcgatatcg ctatatgtta
     3901 taaacaattt tacagttgta tcatctactc gttctattct catatcatct cactccttac
     3961 agtcgatgaa tagtatccac attgtattaa agcatcaaac aaaatacaat ttatttgttt
     4021 gatttctttc tctaaaatat tttggtaact tataataaca tattcgttgt agcaaaacta
     4081 tttaaatgat ttatacagtt aaagcattaa agcactttct taatttaatt ttacctt
//
LOCUS       DAMZKC010000184         4102 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.187, whole genome shotgun sequence.
ACCESSION   DAMZKC010000184 DAMZKC010000000
VERSION     DAMZKC010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4102
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.187"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            31..1071
                     /gene="tdcB"
                     /locus_tag="POB55_002329"
     CDS             31..1071
                     /gene="tdcB"
                     /locus_tag="POB55_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HDI6501272.1"
                     /translation="MTTNTVTLQTAHIVSLGDIEEAKASIKPFIRRTPLIKSMYLSQS
                     ITKGNVFLKLENMQFTGSFKFRGASNKINHLTDEQKEKGIIAASAGNHAQGVALTAKL
                     LGIDATIVMPETAPQAKQQATKGYGAKVILKGKNFNETRLYMEELAKENGMTIVHPYD
                     DKFVMAGQGTIGLEILDDIWNVNTVIVPVGGGGLIAGIATALKSFNPSIHIIGVQSEN
                     VHGMAESFYKRDLTEHRVDSTIADGCDVKVPGEQTYEVVKHLVDEFILVTEEEIEHAM
                     KDLMQRAKIITEGAGALPTAAILSGKINNKWLEDKNVVALVSGGNVDLTRVSGVIEHG
                     LNIADTSKGVVG"
     gene            1102..2424
                     /locus_tag="POB55_002330"
     CDS             1102..2424
                     /locus_tag="POB55_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDI6501273.1"
                     /translation="MSNGKELQKNIGFFSAFAIVMGTVIGSGVFFKISNVTEVTGTAG
                     MALFVWFLGGIITICAGLTAAELAAAIPETGGLTKYIEYTYGDFWGFLSGWAQSFIYF
                     PANVAALSIVFATQLINLFHLSIGSLIPIAIASALSIVLINFLGSKAGGILQSVTLVI
                     KLIPIIVIVIFGIFQSGDITFSLIPTTGNSGNGFFTAIGSGLLATMFAYDGWIHVGNV
                     AGELKNPKRDLPLAISVGIGCIMAVYLLINATFLLTLPIELLAGNLNAASDTSKILFG
                     ENGGKIITIGILISVYGTINGYTMTGMRVPYAMAERKLLPFSHLFAKLTKSGAPWFGA
                     IIQLIIAIIMMSMGAFDTITNMLIFVIWLFYCMSFVAVIILRKREPNMERPYKVPLYP
                     IIPLIAILAGSFVLINTLFTQFILAIIGILITALGIPVYYYKKKQKAA"
     gene            2581..3972
                     /gene="norB"
                     /locus_tag="POB55_002331"
     CDS             2581..3972
                     /gene="norB"
                     /locus_tag="POB55_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter NorB"
                     /protein_id="HDI6501274.1"
                     /translation="MEKPSREAFEGNNKLLIGIVLSVITFWLFAQSLVNVVPILEDSF
                     NTDIGTVNIAVSITALFSGMFVVGAGGLADKYGRIKLTNIGIILNILGSLLIIISNIP
                     LLLIIGRLIQGLSAACIMPATLSIIKSYYIGKDRQRALSYWSIGSWGGSGVCSFFGGA
                     VATLLGWRWIFILSIIISLIALFLIKGTPETKSKSISLNKFDIKGLVLLVIMLLSLNI
                     LITKGSELGVSSLLFITLLAITIGSFSLFIVLEKRATNPLIDFKLFKNKAYTGATASN
                     FLLNGVAGTLIVANTFVQRGLGYSSLQAGSLSITYLVMVLIMIRVGEKLLQTLGCKKP
                     MLIGTGVLIVGECLISLTFLPEILYVICCIIGYLFFGLGLGIYATPSTDTAIANAPLE
                     KVGVAAGIYKMASALGGAFGVALSGAVYAIVSNMTNIYTGAMIALWLNAGMGILSFVI
                     ILLLVPKQNDTQL"
BASE COUNT         1360 a          594 c          773 g         1375 t
ORIGIN      
        1 aaatttacaa aacggaagga tgtaattatt atgacaacca acacagttac attacaaaca
       61 gcacatattg taagtttagg agatatcgaa gaagctaaag caagcattaa accatttatt
      121 cgtcgaacac ctctaattaa atcaatgtat ttaagccaaa gtataactaa agggaatgta
      181 tttctaaaat tagaaaatat gcaattcaca ggatctttta aatttagagg cgctagcaat
      241 aaaattaatc acttaacaga tgaacaaaaa gaaaaaggca ttatcgcagc atctgctggg
      301 aaccatgcac aaggtgttgc tttaacagct aaattattag gcattgatgc aactatcgta
      361 atgcctgaaa cagcaccaca agcgaaacaa caagcaacaa aaggctatgg ggcaaaggtt
      421 attttaaaag gtaaaaactt taacgaaact agactttata tggaagaatt agcgaaagaa
      481 aatggcatga caatcgttca tccatatgac gataagtttg taatggcagg ccaaggaaca
      541 attggtttag aaattttaga tgatatttgg aatgtgaata cagtcatcgt accagttggc
      601 ggtggaggat taattgcagg tattgccacc gcattaaaat catttaaccc ttcaattcat
      661 attatcggtg ttcaatctga gaatgttcat ggtatggctg agtctttcta taagagagat
      721 ttaactgaac atcgagtgga tagcacgata gcagatggtt gtgatgtaaa agttcctggt
      781 gaacaaacat atgaagtagt taaacattta gtagatgaat ttattcttgt tactgaagaa
      841 gaaattgaac atgctatgaa agatttaatg cagcgtgcca aaattattac tgaaggtgca
      901 ggcgcattac caacagctgc aattttaagt ggaaaaataa acaataaatg gcttgaagat
      961 aaaaatgttg ttgctttagt ttcaggcggg aatgttgact taactagagt ttcaggtgtc
     1021 attgaacatg gactgaatat tgcagataca agcaagggtg tggtaggtta aaacatttaa
     1081 tcttaaaaat gaggtgtaat tatgtcaaat ggtaaagaat tacaaaaaaa tataggtttc
     1141 ttctcagcgt ttgctattgt tatggggaca gttattggtt caggagtatt ctttaaaata
     1201 tcaaacgtaa cagaagtaac aggaacagca ggaatggcct tgtttgtatg gttcctaggc
     1261 ggcatcatta ccatttgtgc ggggttaaca gcagcagaac ttgctgctgc aatccctgaa
     1321 acaggtggct taacgaagta tatagaatat acatacggtg atttctgggg cttcctatca
     1381 ggttgggcgc aatcatttat ttattttcca gctaacgtag cagcattgtc tatcgtattt
     1441 gcgacacagc taattaattt attccattta tctataggtt cgttaatacc aatagcaatc
     1501 gcatctgcgt tatctattgt gttgataaat ttcctaggtt caaaagcagg cggaatttta
     1561 caatcagtta ctttagtaat taaactgatt ccaatcatcg ttattgtaat ttttggtatt
     1621 tttcaatctg gagatatcac tttttcatta attccaacta caggtaattc aggaaatggt
     1681 ttctttacag caattggtag tggtttatta gcaactatgt ttgcatatga tggttggatt
     1741 catgtaggaa atgttgcggg ggaacttaaa aatcctaaac gcgatttacc tttagcgatt
     1801 tcagttggta tcggttgtat tatggctgtg tatttattaa ttaacgctac attcttatta
     1861 acgttgccaa tagaactact tgctggtaat ttaaatgcgg cttcagatac atcaaaaata
     1921 ttatttggtg aaaatggcgg taagattatt acaatcggta tattaatttc tgtttatggt
     1981 acgatcaatg gctatactat gactggtatg cgcgtaccat atgcaatggc tgaaagaaaa
     2041 ttattgccat ttagccactt attcgcaaaa ttaacaaaat ctggcgcacc atggtttggc
     2101 gcaattatac aacttataat cgctatcatc atgatgtcaa tgggagcatt tgatacaatt
     2161 acaaacatgc taatctttgt tatttggttg ttctattgta tgtcatttgt tgcggtaata
     2221 attttaagga aacgcgaacc aaatatggag cgaccatata aagtaccgtt atatccaatc
     2281 atacctttaa ttgcaatttt ggcagggtca tttgtattaa ttaatacact gtttacacaa
     2341 tttatactag caatcattgg aattctaata acagcacttg gtataccagt ttattattat
     2401 aagaagaaac aaaaagcagc ataaggtaag ataactagca ttaagaataa atggatggac
     2461 tactaataaa tttaaagttt tacacattaa aatcaaaaac cattcaatta ttctatggaa
     2521 cagacaaatt tctgttatgg aatttgtctg tttttcaaaa gtatagggag gcaaatagag
     2581 atggaaaagc cgtcaagaga ggcatttgaa ggcaataata agttgttaat aggaattgtt
     2641 ttaagtgtaa taacgttttg gctatttgca caatcattgg ttaatgttgt accaatactt
     2701 gaagatagtt tcaatacaga tattggaacg gttaatatcg ccgttagtat aactgcttta
     2761 ttttcaggaa tgtttgtagt aggagcaggt ggtcttgctg ataaatatgg cagaattaaa
     2821 ctcacgaaca ttggtattat cttaaatata ttgggttcat tattaatcat tatttcaaat
     2881 attcctttat tacttattat aggaagatta attcaaggac tttcagcagc atgtattatg
     2941 cctgcaactt tgtctattat taagtcatat tacattggga aagatagaca acgcgcttta
     3001 agttattggt caattggctc atggggcggc tctggtgttt gttcattttt tggaggtgca
     3061 gttgcaacgc ttttaggttg gcgttggatt ttcatcctat caattataat ttcattaatt
     3121 gcgctttttc ttattaaagg cacacctgaa actaaatcta aatcgatttc tctaaataaa
     3181 tttgacatta aaggtctggt tcttttagtc attatgcttc tcagtttaaa tattttaatt
     3241 actaaaggat cagaattagg tgttagctca cttcttttta tcactttatt agcaattaca
     3301 attggatctt ttagtttatt tatagttctt gaaaagcgtg ctacaaatcc tttaatcgat
     3361 tttaaattat ttaaaaataa agcttacaca ggtgcaacag cttcaaactt tttgttaaat
     3421 ggtgttgcag gaacattaat agtagccaac acatttgttc aaagaggttt aggatattct
     3481 tcattgcaag caggaagttt atcaatcact tatttagtaa tggtactaat tatgattcgt
     3541 gttggtgaaa agttacttca aacactcgga tgcaagaaac caatgttaat tggaacagga
     3601 gttcttattg tcggagaatg tctcatttca ttaactttct tgccagaaat attgtatgtc
     3661 atttgttgta ttataggtta tttattcttt ggtttaggac tagggatata tgctacacca
     3721 tcaacagata cagcaattgc aaatgcaccg ttagaaaaag taggcgttgc tgcaggtatc
     3781 tataaaatgg cttctgcatt aggtggagca tttggcgtcg cattgagtgg tgcagtatat
     3841 gcaatcgtat caaatatgac aaacatttat acaggtgcaa tgattgcatt atggttaaat
     3901 gcaggtatgg gaatattatc attcgttatc attttgttac ttgtgcctaa acaaaacgac
     3961 actcaattat gataattgag aattaaattg aaatcataca agtcgctaca atattaaaca
     4021 aaaatataaa ccgattctta tgtgtcatta ttttaaatga acatagggat tggtttttta
     4081 ttactctttt acgctacttt at
//
LOCUS       DAMZKC010000185         4087 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.188, whole genome shotgun sequence.
ACCESSION   DAMZKC010000185 DAMZKC010000000
VERSION     DAMZKC010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.188"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..197
                     /locus_tag="POB55_002332"
     CDS             <1..197
                     /locus_tag="POB55_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MAP domain-containing protein"
                     /protein_id="HDI6501275.1"
                     /translation="IKLKSAIANDQGITKHDIELAERAVYKVYFKNGSSKYVDLKTEY
                     KDERVFKATDIKKVDIELKF"
     gene            complement(521..1807)
                     /locus_tag="POB55_002333"
     CDS             complement(521..1807)
                     /locus_tag="POB55_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDI6501276.1"
                     /translation="MNPLAQSLNEQLQQSNATAFAMLSDLGQNMFYPKGILSQSAEAK
                     STTYNATIGMATNKDGKMFASSLDAMFNDLTPDEIFPYAPPQGIEELRDLWQQKMLRD
                     NPELSIDNMSLPIVTNALTHGLSLVGDLFVNQGDTILLPDHNWGNYKLVFNTRNGANL
                     QTYPIFDKDGHYTTDSLVEALQSYNKDKVIMILNYPNNPTGYTPTHKEVTTIVDAIKA
                     LADKGTKVIAVVDDAYYGLFYEDVYTQSLFTALSNLNSNAILPVRLDGATKEFFAWGF
                     RVGFMTFGTSDQTTKEVLEAKVKGLIRSNISSGPLPTQSAVKHVLKNNKQFDKEIEQN
                     IQTLKERYEVTKEVVYADQYHSHWQAYDFNSGYFMAIKVHDVDPEELRKHLIEKYSIG
                     VIALNATDIRIAFSCVEKEDIPHVFDSIAKAIDDLR"
     gene            2007..2105
                     /locus_tag="POB55_002334"
     CDS             2007..2105
                     /locus_tag="POB55_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501277.1"
                     /translation="MMWWQEGMMTLITGGLLIIFRLWLELKWKNKK"
     gene            2348..2524
                     /locus_tag="POB55_002335"
     CDS             2348..2524
                     /locus_tag="POB55_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501278.1"
                     /translation="MKHLTKIFVVLAIILFIIGYYLQATNHESQGIKLLLAAIMFMIC
                     AFISRSNDRRKNSK"
     gene            2783..3163
                     /locus_tag="POB55_002336"
     CDS             2783..3163
                     /locus_tag="POB55_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDI6501279.1"
                     /translation="MKIILKNNSDFPIYEQIKQQVKQNILKGHVAPGEHLPSMRELAK
                     DLQVSLITTKRAYEDLEKDGFVTTIRGKGTFVKEQDSSILKEKQFFTIENLVKELVNE
                     AQAIEMSLEELQDILTFIYEEESS"
     gene            3160..4056
                     /gene="pmtA"
                     /locus_tag="POB55_002337"
     CDS             3160..4056
                     /gene="pmtA"
                     /locus_tag="POB55_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     ATP-binding protein PmtA"
                     /protein_id="HDI6501280.1"
                     /translation="MNAIELSNVNYSSDQFNLKNISFKVPQGFVTGFIGRNGAGKTTI
                     IRLIMDLYQPQTGVIRVLEEDMALNPIELKNRIGFVYSENYFNERWTTKQLEKMIAPF
                     YRKWDHQVFEFYLEKFDLPINKSIKTFSTGMKMKLSLAVAFSHHAELYIFDEPTSGLD
                     PLARNELLEIIQQELIDENKTIFMSTHIISDLEKIADYIIHLSDGEVILNGSKEQLLQ
                     RYQVVSGAIEDLDDELASLLIYEEHKRTGFIGLTEHAQVFKEILGHKVNIITPSIENL
                     MVYLEKRKPKYHENIKLMEEGF"
BASE COUNT         1412 a          547 c          722 g         1406 t
ORIGIN      
        1 gtataaaact taaatcagca attgcaaatg atcaagggat aactaaacat gacatagaac
       61 ttgctgaacg cgcagtgtat aaagtgtatt ttaaaaatgg ttcgtcaaaa tatgtagact
      121 taaaaactga gtataaagat gaaagagtat ttaaagcaac tgacattaaa aaggtagata
      181 ttgaacttaa attctaatct ttaattttat aataaggcat ctcacaatcg tggggtgcct
      241 tttacatttg tagagatgtg atgcttgaat gtttgaagtg atttgccaaa agttatataa
      301 attggttcta taataaatcg gactgataga aaaagttttt tacttttcat ctgtccgatt
      361 ttttgatttt gttaggagag atgtataaaa ctaattaggt atagtgtttg acaagttgct
      421 gaataaaata tatcttatga tactttgaaa gaaggaataa ttttaaaaat aaaaaaccaa
      481 taatccgagt catactcatc agattattgg ttgaaattaa ttatcttaag tcatcaatcg
      541 ctttagcaat tgaatcaaat acatgtggga tatcttcttt ttctacacag ctaaacgcaa
      601 tacgaatatc tgttgcatta agtgcaataa caccaattga atatttttca attaaatgtt
      661 tacgaagttc ttcagggtca acatcatgaa cttttattgc cataaagtat ccagagttaa
      721 agtcataggc ttgccaatgt gaatgatatt gatcagcata gacaacttcc ttagtgactt
      781 catagcgttc ttttaatgtt tgaatatttt gttcgatttc tttatcgaat tgtttattat
      841 tttttaatac atgcttaaca gcgctttgtg ttggaagagg tccactagaa atgttacttc
      901 gtataagacc ttttactttg gcttctaata cttctttagt tgtttgatca gacgttccaa
      961 atgtcataaa cccaacacgg aatccccatg cgaaaaattc ttttgttgca ccatctaaac
     1021 gaacaggtaa tattgcattt gaatttaaat tagataatgc agtaaataat gattgagtat
     1081 acacatcttc atagaataaa ccatagtatg cgtcatccac aacagctata acttttgtac
     1141 ctttatcagc taatgcttta attgcatcga cgatggtagt cacttcttta tgcgtaggtg
     1201 tgtaacctgt cggattatta ggataattta aaatcataat gactttatct ttattgtatg
     1261 attgtaaagc ttctacaagt gaatcagtag tataatgccc gtctttatca aagataggat
     1321 atgtttgaag gtttgcacca tttctcgtat tgaaaacaag tttgtaatta ccccaattat
     1381 gatctggtaa caagatagtg tcaccttgat ttacaaataa atcgccaact aaagataaac
     1441 catgtgttaa tgcattcgta acaattggta gtgacatgtt gtcgattgat agctctggat
     1501 tgtcacgcaa cattttttgt tgccataaat cacgtaattc ttcgatgcct tgtggtggcg
     1561 cataagggaa tatttcatct ggagttaaat cattaaacat tgcatctaaa gatgatgcaa
     1621 acatttttcc gtctttgttt gtcgccatac ctatagttgc attatatgtt gtactctttg
     1681 cttcagcaga ttgagataaa atgccttttg gataaaacat attttgacct aagtcagata
     1741 acattgcaaa ggcagttgca tttgactgtt gaagttgttc atttaagctt tgggctaaag
     1801 gattcattat tgcttaacct ctctttaaaa aaattgtatg cctatatatt atcttcaatt
     1861 ataccaaaga taaagtgttg ttggtgaatt aacgaataat atgtaaaatc tgctgccaaa
     1921 atacgaacat atgttctgtt tttatgttag aataggtgta ggtcatcctt cagtatgata
     1981 ctttagtcga acggaggtgt agtgtaatga tgtggtggca agaagggatg atgacactta
     2041 ttacaggtgg cttactaatt atttttcgac tttggttaga actgaaatgg aaaaataaaa
     2101 aatgatccta cacacataaa taaccccaag tctgctggta ccagacttgg ggttgtgtag
     2161 tataattctg tggatacact taaataatat cattgaacac aatatatcgc aatcaataat
     2221 cattttgatt gaatggtttt gttgcgctat tttagttgaa agtgattagt accttgaact
     2281 tttgaaattc aataacttat taaaaaaatt tatatttgtt ataatgtcga tgttgaggtg
     2341 aaataaattg aaacatctaa caaaaatatt tgtagtactt gcaattattt tatttattat
     2401 tggttattat ttacaagcaa ctaatcatga aagccaaggt ataaaattat tattagcagc
     2461 gattatgttt atgatttgtg cttttataag tagaagtaat gatcgacgta aaaatagtaa
     2521 atagaattga ctgtttaaaa cgattgttta tgttgacgct acggttgtca cgtagagatt
     2581 aaagacattc gttttagcag tcttttttat tgaagtagac aatgcaagat taaatggtag
     2641 tgtcatttca ttttattcaa ctgctagtaa tacaatactc agtgttattt ctgaagactg
     2701 gttgataatg agacatgcat atttacaaaa ctgtgtatat tgtgtatatt gtatatatac
     2761 agaaagtgat agggggacgt tgatgaaaat aattttaaaa aacaatagtg attttccgat
     2821 ttatgaacag attaagcaac aagtaaaaca aaatatttta aagggacatg ttgctcctgg
     2881 agagcatttg ccgtcaatga gagaacttgc caaagatctt caagtaagtt tgattactac
     2941 caaacgtgct tatgaagatt tagagaaaga cggttttgtt acaacaatta gaggaaaagg
     3001 gacctttgtt aaggagcaag atagttctat tttaaaagag aaacaatttt ttaccattga
     3061 aaatttggtt aaagaattgg ttaatgaagc gcaagccatc gaaatgtcac ttgaggaact
     3121 tcaagatatt ttaacgttca tttatgagga ggaatcatca tgaatgccat agaattaagt
     3181 aatgttaatt attcaagtga tcagtttaat ttaaagaata tctctttcaa agttcctcaa
     3241 ggattcgtca ctggttttat tggtagaaat ggcgctggta aaaccacaat aataaggtta
     3301 attatggatt tgtatcaacc acaaactggt gttattagag tattagaaga ggatatggct
     3361 ctcaatccga tagagttaaa gaatagaatt gggtttgttt actctgaaaa ttattttaat
     3421 gagagatgga ctacgaagca acttgaaaaa atgattgccc ctttttatcg taaatgggac
     3481 catcaagtat ttgaatttta tcttgaaaag ttcgatttac caatcaataa atcaataaaa
     3541 acattttcga ctgggatgaa aatgaaatta tcactagcag ttgcattttc acatcatgct
     3601 gaattgtata tttttgatga gcctacttca ggtcttgatc ccttagctag aaatgagcta
     3661 ttggagataa ttcagcaaga attaattgat gaaaataaaa caatattcat gtcgacacac
     3721 attatctcag atttagaaaa aatagctgat tatatcattc acttaagtga cggtgaagtt
     3781 atcttaaatg gttcaaaaga acaactgtta caaagatatc aagttgtgag tggtgctatt
     3841 gaggaccttg atgatgaatt agcatctcta cttatttatg aagaacataa gcgtacggga
     3901 tttataggat taacagaaca tgcgcaagta tttaaagaaa ttttaggtca taaagtgaat
     3961 atcattacac cttctattga gaacttaatg gtttatcttg aaaagagaaa acctaaatat
     4021 catgaaaaca tcaaattgat ggaagaaggt ttttaaatga aggcattgtt aattaggaat
     4081 tttaaat
//
LOCUS       DAMZKC010000186         4086 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.189, whole genome shotgun sequence.
ACCESSION   DAMZKC010000186 DAMZKC010000000
VERSION     DAMZKC010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4086
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.189"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(7..155)
                     /locus_tag="POB55_002338"
                     /pseudo
     CDS             complement(7..155)
                     /locus_tag="POB55_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495236.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(511..933)
                     /locus_tag="POB55_002339"
     CDS             complement(511..933)
                     /locus_tag="POB55_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501281.1"
                     /translation="MKSVNILLNLLPTELKNMLENKDMENILTYFMSNEISDEKLVSY
                     LSNLANQINTIEYHEMVASIYHFHFNYIDNAYDLAYYHYWQSLEISQFNNANLLYEFL
                     EILGEPDFNMISHENIQLVANKILERDPNNKLAIKYIN"
     gene            complement(934..1521)
                     /locus_tag="POB55_002340"
                     /pseudo
     CDS             complement(934..1521)
                     /locus_tag="POB55_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801062.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB-like protein"
     gene            complement(1787..1939)
                     /locus_tag="POB55_002341"
     CDS             complement(1787..1939)
                     /locus_tag="POB55_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501282.1"
                     /translation="MVPEEKGSITLSKEAAIIFATAKFKPFHNRIKNNPQKTNPFLKL
                     HENKKS"
     gene            2302..2709
                     /locus_tag="POB55_002342"
     CDS             2302..2709
                     /locus_tag="POB55_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2992 family protein"
                     /protein_id="HDI6501283.1"
                     /translation="MELSIFYNGQFFVALVEYKMENKSKFIQYTFGNEPDDIEVLDFI
                     HHQLMKMIDDVQTIVYTKNISRKVNPKKLQRQIAKEQKKPKYSTQAQIAIKKELELKK
                     KQKRKRYKEKRDAFQKRKREIKKVKAKEKHKGH"
     gene            2756..3238
                     /locus_tag="POB55_002343"
     CDS             2756..3238
                     /locus_tag="POB55_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000600874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HDI6501284.1"
                     /translation="MILISSCLIGDNVRYDGGNQLNVRLKKLIDSGKAIHACPELLGG
                     LLIPREPAEIIGGDGFDVWNDAAKVVTISNKDVTDDYKHGAIVTLKILKKYQCDTVIL
                     KANSPSCGSQEIYDGNFTGNKKKGVGVATALLINEGIKVYDENTFFDQNMNETIVHEK
                     "
     gene            3438..>4086
                     /locus_tag="POB55_002344"
     CDS             3438..>4086
                     /locus_tag="POB55_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HDI6501285.1"
                     /translation="MEKVAILVDGGYYRKISAKVYGKVTAKERADELYSYCNRHLKET
                     HFKEEIYNKLYRIFYYDCPPIDKIVYHPLLKKNVNFSNTDTKKWTEDFFKEMSKKRKV
                     ALRLGELSEYSVEYNLKYSITKKLLNGSIDLNDLKEKDFSLSLQQKGVDMKIGLDIAS
                     LSFKHQVDKIILIAGDSDFVPAAKLARTEGIDFVLDSLGADIRNNLSLHIDGRRTC"
BASE COUNT         1433 a          498 c          689 g         1466 t
ORIGIN      
        1 acgctagttg atggtatctt taaagtgtgt attacctgat tgatatcaat accaggtatc
       61 gaatgaatat ttttaaaata attgagttgt tgtgctaatt gaatcatggc atcatttaat
      121 tgtttgatac gatggataga ttgttttaat tgtttaggcg gcctttgttg gtcgccattt
      181 ttatataata atataaatga ccaaaccaca gcacctatta atttaagagt gaggtcattt
      241 ttaaaggtaa aaaaggagta attttatata ttaaaatgat atataaaaca agtagtatta
      301 gtatgttgtg aattgttata gaatttgact tgttagcaat ttcgtagtta ttgtaatagc
      361 aagtactgta tggttttatt ttttaaaggt agtgaaataa tattggattt ttgaagtgaa
      421 atatttgtag atggatacaa cgaagcatat tagaaataca aaaggaacga tgaaattaaa
      481 tgtccatcat tccttatgcc tagtagtttt ttagttaata tatttaattg ctaacttatt
      541 attagggtct ctttcaagaa ttttatttgc gaccaattga atattctcat gtgaaatcat
      601 attaaagtct ggttctccaa ggatttctaa aaattcgtat aataaattag cattattaaa
      661 ttgcgaaatc tctaacgatt gccaataatg atagtatgct aaatcatagg cattatctat
      721 gtaattgaaa tgaaagtgat agatgcttgc gaccatttca tgatattcaa ttgtatttat
      781 ttgattcgca agatttgata aatatgatac taatttttca tctgaaattt cgttcgacat
      841 aaagtatgtt agaatattct ccatatcttt attctctaac atatttttta attcagttgg
      901 taataaattt aatagaatgt taacgctttt catttattta acccatccat tcgatatata
      961 tattgccatt ttttcgtgta taagttatga ctacagttct atcactcatg acaagtgttt
     1021 tttggtatgc ggactttttg tatcggcttt gctttccata cctcataaca tgcgacatta
     1081 ctttagaaac atctttccaa ttgtttttag taactttatt ccaattatgt ttactagcaa
     1141 gaatatgttt tcttttatta gctcctagat tggctacaga aggtaaaaat ttaccaaaac
     1201 tctttgtttt gatagcggtt tgagttgctt tttttccata tgttttaatt gcatagttta
     1261 ttccgtatca gctactgcag ctaatattac taatggcaat gcagaagatt tagcttcaat
     1321 tgagttgaat tttattattt cgccagtttc tttgtcgatt aatgtagctt catattcttc
     1381 attttcaaat tgttctacga aaacgtcata atccttattt attagttttc cattttcatc
     1441 gacgtatctg cccttatcat ccatagtatt agtattcaaa tcatattgaa tatttttatc
     1501 taaatcaata tcttttttcg tttttccact aacgtctttt tcaagcttta tatttacaat
     1561 ttttaagcag aagtatccca ttatttcaag gtctgattta tctataatta aatacgtacg
     1621 tatggaattt tgatcgttca tttttattgc tttatgtttt aggaaatgtg ctattgaagg
     1681 gtttaaagat gtttggaatt tcttggtttc ttgacttata tagttatgaa agagggcgtt
     1741 gtcctttatt aaatttctga gatcagaaat gctataaata aagtgattaa gattttttgt
     1801 tttcatgtaa tttaagaaat ggatttgttt tttgtgggtt atttttaatt ctattatgaa
     1861 atggtttgaa ttttgcagtt gcaaatatga tagctgcttc ttttgacaaa gtaatagaac
     1921 ctttttcttc tggtaccatg atattcactc cttgtacaat tcaatttaag tattataaaa
     1981 taattttact atacaattac ctttgtgtaa atcgtttctt attgttacca aattatttgt
     2041 ttaaaagcat tatgcaatgc caatcaacac taatggggtg taagtatttc cctgttaata
     2101 ttttattagc gttatttaca tataccttct gtaatgtgaa tttttaaaat tacagtttac
     2161 atgtaacgtt gtgcaatata ttctgtatat agaagtgtaa aaacacaatt gggttggtag
     2221 tcccaatgca gttttaaaac tgtcagtaac cttcctcctc gggtcgtccg tcatttctaa
     2281 taaaactaga ggagggatag catggagcta tctatttttt acaatgggca attttttgta
     2341 gcattggtag aatataaaat ggaaaataaa tctaaattta tccaatacac atttgggaat
     2401 gaacctgatg atatagaggt attggatttt attcatcatc aattaatgaa aatgattgat
     2461 gatgtgcaaa ctattgttta tacgaaaaat atttctagaa aagtaaaccc gaaaaaacta
     2521 caacgacaaa ttgctaagga gcaaaagaaa cctaaatatt ctacccaagc acaaatagct
     2581 attaagaaag aattagaatt aaagaaaaag caaaagcgga agcgctataa agaaaaacgt
     2641 gatgcatttc aaaaaagaaa aagagaaatt aaaaaggtta aagcaaaaga gaagcataag
     2701 gggcattagc gatacgacaa ataaattaaa acaaatagat ttataggtgg ttgggatgat
     2761 tttaattagt tcgtgtttga taggcgataa tgtaagatac gatggtggta atcaattgaa
     2821 tgttagattg aaaaaattaa tagacagcgg aaaagctatt cacgcatgtc cagaattact
     2881 tggtggatta ttaattccta gagaacctgc agaaattatt ggtggcgatg gttttgatgt
     2941 atggaatgac gctgcaaagg ttgttactat ttcgaacaaa gacgtaactg acgattataa
     3001 acatggagca atagttacat taaaaatttt gaaaaaatat caatgtgata cagttatttt
     3061 aaaagcaaat agcccatcat gtggatcaca agagatatat gatgggaatt ttacaggaaa
     3121 taagaaaaag ggtgtaggtg tggcaactgc tttactcatt aatgaaggta taaaagttta
     3181 tgatgaaaat acgttttttg accaaaacat gaatgaaacg atagtacatg aaaagtaagt
     3241 attgaggata tacgtaataa accaaacatt taagttgtaa gatactttat aaaatatttt
     3301 ttaaaatcgt tgaaatgata ttcaagttgt gataatatat cggtacagat aattttgtta
     3361 acagatccat gagagtagga accccaccat acgggaattt cgaaatcatg gattttttga
     3421 tataaaggag tgatgtaatg gaaaaagtag ccattttagt agatggtgga tactatagaa
     3481 agataagcgc aaaagtctac ggaaaagtta cagcgaaaga aagagctgac gagttatata
     3541 gttattgcaa tagacactta aaagagacac atttcaaaga agaaatatat aataaattat
     3601 acagaatatt ttactatgat tgtcctccta ttgataagat agtttatcac cccttattaa
     3661 aaaagaatgt aaatttttcc aatactgata cgaaaaagtg gacagaagac tttttcaaag
     3721 aaatgagcaa aaaaagaaag gtagcattga ggttaggaga gttgagtgag tattcagttg
     3781 aatacaactt aaaatactct attacaaaaa aattattaaa tggaagtatc gatttaaatg
     3841 atttgaaaga aaaagatttt tccctatcat tacaacaaaa aggtgttgat atgaaaatag
     3901 gtttagacat agcttcatta tcatttaaac atcaagtaga taaaattatt ttaatagctg
     3961 gtgatagcga ttttgtacct gctgctaaac tagcaagaac agaaggaatt gattttgtat
     4021 tagattcttt aggggcagat attagaaaca acttgtcatt acacattgat ggtaggcgta
     4081 cttgtg
//
LOCUS       DAMZKC010000187         4051 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.190, whole genome shotgun sequence.
ACCESSION   DAMZKC010000187 DAMZKC010000000
VERSION     DAMZKC010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4051
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.190"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            63..947
                     /gene="ylqF"
                     /locus_tag="POB55_002345"
     CDS             63..947
                     /gene="ylqF"
                     /locus_tag="POB55_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HDI6501286.1"
                     /translation="MVIQWYPGHMAKAKREVSEQLKKVDVVFELVDARIPYSSRNPMI
                     DEVINQKPRVVILNKKDMSNLNEMSKWEQFFIDKGYYPVSVDAKHGKNLKKVEAAAIK
                     ATAEKFEREKAKGLKPRAIRAMIVGIPNVGKSTLINKLAKRSIAQTGNKPGVTKQQQW
                     IKVGNALQLLDTPGILWPKFEDEEVGKKLSLTGAIKDSIVHLDEVAIYGLNFLIQHDL
                     ARLKSHYNIEVPEDAEIIAWFDAIGKKRGLIRRGNEIDYEAVIELIIYDIRNAKIGNY
                     CFDIFKDMTEELANDANN"
     gene            931..1698
                     /locus_tag="POB55_002346"
     CDS             931..1698
                     /locus_tag="POB55_002346"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HDI6501287.1"
                     /translation="MTLTIKEVKQLINAVNTIEELENHECFLDERKGVQNAIARRRKA
                     LEKEQALKEKYVEMTYFENEILKEHPNAIICGIDEVGRGPLAGPVVACATILNSNHNY
                     LGLDDSKKVPVTKRLELNEALKNEVTAFAYGIATAEEIDEFNIYKATQIAMQRAIDGL
                     SVQPTHLLIDAMTLDNALPQVSLIKGDARSVSIAAASIMAKVFRDDYMTQLSKDYPEY
                     GFEKNAGYGTKQHLLAIDDIGIMKEHRKSFEPIKSLL"
     gene            1807..2973
                     /gene="sucC"
                     /locus_tag="POB55_002347"
     CDS             1807..2973
                     /gene="sucC"
                     /locus_tag="POB55_002347"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HDI6501288.1"
                     /translation="MNIHEYQGKEIFRSMGVAVPEGRVAFTAEEAVEKAKELNSDVYV
                     VKAQIHAGGRGKAGGVKIAKSLSEVETYAKELLGKTLVTHQTGPEGKEIKRLYIEEGC
                     AIQKEYYVGFVIDRATDQVTLMASEEGGTEIEEVAAKTPEKIFKETIDPVIGLSPFQA
                     RRIAFNINIPKESVNKAAKFLLALYNVFIEKDCSIVEINPLVTTADGDVLALDAKINF
                     DDNALFRHKDVVELRDLEEEDPKEIEASKHDLSYIALDGDIGCMVNGAGLAMATMDTI
                     NHFGGNPANFLDAGGSATREKVTEAFKIILGDENVKGIFVNIFGGIMKCDVIAEGIVE
                     AVKEVDLTLPLVVRLEGTNVELGKKILKDSGLAIEPAATMAEGAQKIVKLVKEA"
     gene            2995..3903
                     /gene="sucD"
                     /locus_tag="POB55_002348"
     CDS             2995..3903
                     /gene="sucD"
                     /locus_tag="POB55_002348"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HDI6501289.1"
                     /translation="MSVFIDKNTKVMVQGITGSTALFHTKQMLDYGTKIVAGVTPGKG
                     GQVVEGVPVFNTVEEAKNETGATVSVIYVPAPFAADSILEAADADLDMVICITEHIPV
                     LDMVKVKRYLQGRKTRLVGPNCPGVITADECKIGIMPGYIHKKGHVGVVSRSGTLTYE
                     AVHQLTEEGIGQTTAVGIGGDPVNGTNFIDVLKAFNEDDETKAVVMIGEIGGTAEEEA
                     AEWIKANMTKPVVGFIGGQTAPPGKRMGHAGAIISGGKGTAEEKIKTLNSCGVKTAAT
                     PSEIGSTLIEAAKEAGIYEALLTVNK"
BASE COUNT         1484 a          548 c          871 g         1148 t
ORIGIN      
        1 ataattaaca ggttatagat tttaataatg agaaaagaat tgacgaaagt aaggtgaatt
       61 gaatggttat tcaatggtat ccaggacata tggcgaaagc caaaagagaa gtaagtgaac
      121 aattaaaaaa agtagatgta gtgtttgaac tagtagatgc aagaattcca tatagttcaa
      181 gaaaccctat gatagatgaa gttattaacc aaaaaccacg tgttgttata ttaaataaaa
      241 aagatatgtc taatttaaat gagatgtcaa aatgggaaca attttttatt gataaaggat
      301 actatcctgt atcggtggat gctaagcacg gtaaaaattt aaagaaagtg gaagctgcag
      361 caattaaagc gactgctgaa aaatttgaac gtgaaaaagc aaagggactt aagccaagag
      421 cgattagagc gatgattgtt ggaattccaa atgttggtaa atccacatta ataaataaac
      481 tggcaaagcg tagtattgcg cagactggta ataaaccagg tgtgaccaaa caacaacaat
      541 ggattaaagt tggtaatgca ttacaactat tagacacacc agggatactt tggcctaaat
      601 ttgaagatga agaagtcggt aagaagttga gtttaacagg cgcgattaaa gatagcattg
      661 tgcacttaga tgaagtagct atatatggat tgaacttttt aattcaacat gatttagcgc
      721 gattaaagtc acattataat attgaagttc ctgaagatgc agaaatcata gcgtggtttg
      781 atgcgatagg gaaaaaacgt ggcttaattc gacgtggtaa tgaaattgat tacgaagcag
      841 tcattgaact gattatttat gatattcgaa atgctaaaat aggaaattat tgttttgata
      901 tttttaaaga tatgactgag gaattagcaa atgacgctaa caattaaaga agttaagcag
      961 ttgattaatg cggttaatac aatagaagaa ttagaaaatc atgaatgctt tttagatgag
     1021 cgaaaaggtg ttcaaaatgc catagctagg cgcagaaaag cgttagaaaa agaacaagct
     1081 ttaaaagaaa agtatgttga aatgacttac tttgaaaatg aaatattaaa agagcatcct
     1141 aatgcaatta tttgtggtat tgatgaagtt ggaagaggac ctttagcagg tccagtcgtt
     1201 gcatgcgcaa caattttaaa ttcaaatcac aattatttgg gccttgatga ctcgaaaaaa
     1261 gtacctgtta cgaaacgtct agaattaaat gaagcactaa aaaatgaagt tactgctttt
     1321 gcatatggta tcgcgacagc tgaagaaata gatgaattta atatttataa agctactcaa
     1381 atcgccatgc agcgagctat tgatggatta tcagtacaac caacgcattt attgatagac
     1441 gcgatgacgc ttgataatgc actgcctcaa gtatctttaa tcaagggtga tgcaagaagt
     1501 gtatctattg cagcggcaag tatcatggca aaggtttttc gtgatgatta tatgacacag
     1561 ttatctaaag attatcctga atatggtttt gaaaaaaacg cgggttacgg taccaaacaa
     1621 catttactag caatcgatga tattggcatt atgaaagagc atagaaaaag ctttgaacct
     1681 ataaaatcgt tactgtaaat cgtaaaattc aattaaatga taaaaaagta gcatttaagt
     1741 ttacaatagc gcttacattt tttataattg taagggaact taacctaagt aacaggagga
     1801 tggaagatga atatccacga gtatcaaggt aaagaaatat ttcgttcaat gggcgttgca
     1861 gttccagaag gacgagtagc atttactgct gaagaagcgg tggagaaagc aaaagaatta
     1921 aattctgatg tttatgttgt aaaagcacaa attcatgctg gaggtagagg taaagcaggc
     1981 ggagtaaaaa ttgctaaatc tttatctgag gtagaaacat atgcaaaaga attattaggg
     2041 aaaactttgg tgacacatca aactggtcca gaaggtaaag aaattaagcg tttatatatc
     2101 gaagaaggtt gtgctattca aaaagaatat tacgttggat tcgttattga tcgtgcgact
     2161 gaccaagtaa cattgatggc gtctgaagaa gggggcactg agattgaaga agttgctgcg
     2221 aagactcctg aaaagatctt caaagaaact atcgatccag taatcggact ttcaccattc
     2281 caagcaagac gaattgcgtt taatattaat attcctaaag aatctgttaa caaagcagct
     2341 aaattcttat tagcacttta taatgtattc attgaaaaag attgttcaat cgtagaaatc
     2401 aacccattag ttacaacagc tgatggtgat gtattggcat tagatgctaa aattaatttt
     2461 gatgataatg cattattcag acataaagat gttgtagaat tacgtgattt agaagaagaa
     2521 gatccgaaag agattgaagc gtctaaacat gatttatcat acattgcatt agatggtgac
     2581 atcggatgta tggttaatgg tgcaggttta gccatggcaa caatggatac gattaatcat
     2641 ttcggtggaa acccagccaa tttcttagat gcaggcggaa gcgctactag agaaaaagta
     2701 actgaagcat ttaaaatcat tttaggtgat gaaaatgtta aaggtatttt tgtaaacatt
     2761 ttcggtggca ttatgaaatg tgatgttatc gcagaaggta tcgttgaagc tgtaaaagaa
     2821 gtagatttaa ctttaccact agttgtacgc ttagaaggta caaatgttga gttaggtaaa
     2881 aaaatcttaa aagactcagg attagcaatt gaaccagcag caacaatggc tgaaggtgca
     2941 caaaaaattg ttaaactagt caaagaagca taaggaaagg atgggagcac taagatgagt
     3001 gtatttatag ataagaatac taaagtaatg gtacaaggta ttacagggtc tactgccctt
     3061 ttccatacaa aacaaatgct tgattatggt acgaaaatag tagcaggtgt gacgcctggt
     3121 aaaggtggtc aagttgttga aggcgttcct gttttcaaca ctgttgaaga agctaaaaat
     3181 gaaaccgggg caacggtttc agtcatttac gttccagcac catttgctgc agactcaatt
     3241 ttagaagcag ctgatgcaga cttagatatg gttatttgta tcactgaaca tattcctgta
     3301 ttagacatgg ttaaagttaa acgctactta caaggtagaa aaacacgttt agttggtcca
     3361 aactgtccag gtgtgattac agcagatgaa tgtaaaattg gtattatgcc tggctatatt
     3421 cacaaaaaag gtcatgttgg tgtagtatct cgttcaggta cattaacata tgaagcagtg
     3481 caccaattga ctgaagaagg tattggtcaa actacagctg ttggtattgg tggagaccca
     3541 gtcaacggaa caaactttat tgatgtttta aaagcattca atgaagatga cgaaacgaaa
     3601 gcagttgtta tgattggtga aatcggtggt acggctgaag aagaagcagc tgaatggatt
     3661 aaagcgaata tgacaaaacc agttgtaggc tttatcggtg gacaaacagc acctcctgga
     3721 aaacgtatgg gacatgctgg tgcaatcatt tcaggtggta aaggtactgc tgaagagaaa
     3781 attaaaacat taaatagttg tggtgtgaaa acagcggcaa caccttcaga aattggttca
     3841 acattaattg aagctgctaa agaagcaggt atttatgaag cattattaac tgttaataaa
     3901 taaagttaaa agatgatata aatggctata gcccattcca tactttataa aagtattgga
     3961 aatgggctat ttgtcgtgcg atgaatttta gtacactttt catattttgg aaagacaagt
     4021 taattatttt acctcctaca atagctttac a
//
LOCUS       DAMZKC010000188         3867 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.191, whole genome shotgun sequence.
ACCESSION   DAMZKC010000188 DAMZKC010000000
VERSION     DAMZKC010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3867
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.191"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(<1..1367)
                     /locus_tag="POB55_002349"
     CDS             complement(<1..1367)
                     /locus_tag="POB55_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HDI6501290.1"
                     /translation="MLHDILRNKFGFESFKPGQQEIIESIMSQQHTLGILPTGSGKSL
                     CYQIPTYLSGKPTLIISPLISLMDDQVMQLKINGEKRVTCIHSGMDEIEKKHNIKCLR
                     HSRFIFLSPEFLLQPSNFKLISMIDFGMIVLDEAHCLSEWGYDFRPHYALIGKVTKHF
                     KEAVVLALTATAPPHLQDDLTEMLAIQFNVIKTTMNRPNISFKHLNFHDDEDKIEWLL
                     PFLQQSGPTIIYVSSKKMCLNLAQLIYDSGFLTGIYHGDMNYQERHTVQQQFLNNDIP
                     IIVATSAFGMGINKKDIRTIIHFHLSTSPSNYIQEIGRAGRDGELSQAISLFQPDDKY
                     ILETLLFADMITEEDVQNFEIGEFLAPDKQDVLTTLHSFYSIGALKQIFKQSFKRKQL
                     GFFRMIGYCKLDQCRRKYLLEFFGEYPPAQDRCCDNDSNITDIAILNKKKVIRSIGFD
                     EKLQN"
     gene            complement(1357..2310)
                     /locus_tag="POB55_002350"
     CDS             complement(1357..2310)
                     /locus_tag="POB55_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI6501291.1"
                     /translation="MQHIIKTALQQTFNYKTNKSIYNILVGKKSHQTFFDACSQQQLS
                     LYHSLPLLKYPSFELFLEKINEFNAEMEIMLHPRYTFESMGQTFQAIQLLVQTMSNTK
                     QHVFHFVPISQNNKIQETVKIVYNYIKENQLQIDFENELHNLFKAITLKGPCYLHYYL
                     QGYDEPMYTRQQVSLIEKLSQQQLFEYEMNNLVTMMFELESGEYTILSKIIMKPTLLN
                     QTYITYTKLLEQFTMEDIAAQQQVKINTIEDHVLEILIKGYMSNYDDYVELEDQLQFL
                     NFYQQHRGERLKFYKEQFDTLSYFQLKVLIVGFERGDLNVA"
     gene            2418..2666
                     /locus_tag="POB55_002351"
     CDS             2418..2666
                     /locus_tag="POB55_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002449743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HDI6501292.1"
                     /translation="MAKYTIVDMDTCIACGACGAAAPDIYDYDDEGIAFVILDDNQGT
                     AEVPEELYEDMEDAIDGCPTDSIKIADESFDGDALKFE"
     gene            complement(2772..3317)
                     /locus_tag="POB55_002352"
     CDS             complement(2772..3317)
                     /locus_tag="POB55_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDI6501293.1"
                     /translation="MQQNKRLITISMLSAIAFVLTFIKFPIPFLPPYLTLDFSDVPSL
                     LATFTFGPVAGIVVALVKNLLNYLFSMGDPVGPFANFLAGASFLLTAYAIYKNKRSTK
                     SLITGLIIATIVMTIVLSILNYFVLLPLYGMIFNLADIANNLKVIIVSGIIPFNIIKG
                     IVISIVFILLYRRLANFLKRI"
     regulatory      complement(3379..3513)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(3727..>3867)
                     /locus_tag="POB55_002353"
     CDS             complement(3727..>3867)
                     /locus_tag="POB55_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 family protein"
                     /protein_id="HDI6501294.1"
                     /translation="SEVLKKKKNIPHDLNVSLQISNKYINTTRPNYSKKDVIEVGVFN
                     HE"
BASE COUNT         1257 a          617 c          606 g         1387 t
ORIGIN      
        1 aaattttgca acttttcatc aaatccaata cttctaatta ccttcttctt atttaaaatt
       61 gcgatatcag ttatattaga atcattgtca caacatcgat cttgtgccgg tggatattca
      121 ccgaaaaatt ctaataaata cttccgtcta cattgatcca atttgcaata gccaatcatg
      181 cgaaagaatc ctaattgctt tcgtttaaat gattgcttaa atatctgttt caaggcgccg
      241 atactataga atgaatgcaa cgttgtcaaa acgtcttgtt tatcgggagc taaaaattct
      301 cctatttcga aattttgtac atcttcttct gttatcatat ctgcaaataa taacgtttct
      361 aaaatatatt tatcgtccgg ttggaataaa ctaattgcct gacttagttc accatcgcga
      421 cccgcacggc caatttcttg aatgtagtta gaaggacttg ttgaaagatg aaagtgaatg
      481 attgtgcgaa tatctttttt attaattccc ataccaaaag cactcgttgc gactataatc
      541 ggaatatcat tatttaaaaa ttgttgttga actgtgtgtc gctcttgata attcatatca
      601 ccatgataaa tacctgtaag aaaacctgaa tcataaataa gttgcgctaa attcagacac
      661 atctttttcg atgagacata aataatcgtt ggtcccgact gttgtagaaa cggcagcaac
      721 cactcaattt tatcttcgtc atcatgaaaa ttaagatgct taaagcttat gtttgggcga
      781 ttcattgtag ttttaataac gttaaattga atcgctaaca tttccgtcaa atcatcttgt
      841 aaatgcggtg gtgcagttgc tgtcaatgct aagacaaccg cttctttaaa atgctttgtt
      901 acttttccta ttagagcata atgtggtctg aaatcatatc cccattcaga taggcaatgt
      961 gcttcatcta gaacaatcat gccaaagtct atcatagata ttaatttaaa atttgacggt
     1021 tgcaagagaa attctggact tagaaagatg aagcggctat gtcgtaaaca tttaatatta
     1081 tgctttttct caatttcatc cataccagag tgaatacatg ttacacgttt ttctccattt
     1141 attttcaact gcataacttg gtcatccatt aaagatatta acggtgagat aattaatgtc
     1201 ggcttacctg ataaatacgt aggtatttga taacacaaac tctttccact tccagttgga
     1261 agtataccta gagtgtgttg ttgagacatt atactttcta taatttcctg ttgtcccggt
     1321 ttaaaactct cgaatccaaa tttgtttcgt aaaatatcat gcaacattca gatcacctct
     1381 ttcaaatcca acgattaata cttttaattg aaaatatgat aacgtgtcaa attgttcttt
     1441 gtaaaatttt aatcgttcgc cacgatgctg ttgataaaaa ttcaaaaact ggagttgatc
     1501 ttctagttca acataatcat cgtaattaga catgtaacct ttgattaaga tttcaagtac
     1561 atgatcttcg atagtattga ttttaacttg ttgttgagcc gctatatctt ccatcgtgaa
     1621 ttgttcaagc aattttgtat aagtaatata agtttgattt aataatgtag gtttcattat
     1681 tatttttgat aaaatagtat attctccact ttctaattca aacatcattg tcactaaatt
     1741 attcatttcg tattcaaaca attgctgttg agatagcttt tctattaaac taacttgctg
     1801 tctcgtatac attggttcat catagccttg caaataataa tgtaagtaac atggcccttt
     1861 taaagttatt gctttaaaca agttatgtag ttcattttca aaatcaattt gtaattggtt
     1921 ttctttaatg tagttataga caatctttac agtttcttgt atcttattat tttgagagat
     1981 tggtacaaaa tgaaaaacat gttgtttggt attagacatg gtttgcacta atagttgaat
     2041 tgcttgaaat gtttgcccca tgctttcaaa tgtatatcta ggatgcaaca tgatttccat
     2101 ttcagcatta aattcattga ttttttctaa aaatagctca aaagacggat attttaatag
     2161 tggtaaactg tgatataatg acaactgttg ttgactacaa gcgtcaaaaa aggtttggtg
     2221 agatttctta ccaactaaga tattgtaaat acttttattt gttttatagt taaatgtttg
     2281 ttgtaatgct gtttttataa tgtgttgcaa atttaaccct cactctgtaa aacggataat
     2341 ttgaaaataa aaatgatgct gtctaaaatt ttagtcacat tactatttta gcatttttat
     2401 caaaggaggc gaagacattg gcaaaatata caatcgttga tatggatact tgtattgcat
     2461 gtggtgcatg cggtgcagca gcgccagata tatatgatta cgacgacgaa ggtattgctt
     2521 tcgtaatcct tgacgataac caaggtactg cagaagtacc tgaggaatta tatgaagata
     2581 tggaagatgc aattgatgga tgccctacag attctattaa aattgcagac gaatcatttg
     2641 atggggacgc tttaaaattt gaataattga ttttttagac aaatgttcta tagaaaaagc
     2701 aggcaagcta tcgtttattg atagtcacct gctttttatt attatgtgta tattattttg
     2761 ctttaatttg attaaattct tttcaagaaa ttcgcaagcc ttctatatag taaaataaat
     2821 acaatagaaa taacgatacc tttaataata ttgaatggta taattcctga aacaatgatt
     2881 actttaagat tatttgcgat atcagctaag ttaaatatca taccgtacaa aggtaataga
     2941 acgaaatagt tcaaaatact caacacgata gtcataacga ttgttgcaat gattaatcca
     3001 gtaatcaaag attttgttga acgtttattt ttatagatgg cgtaagcagt taataagaaa
     3061 cttgcgcctg ctaaaaagtt agcaaatggt ccaactggat cgcccatact aaataagtag
     3121 ttcaataaat ttttaaccag tgcaactacg ataccggcaa ctggtccaaa cgtaaatgta
     3181 gctagtagtg acggtacatc actaaaatct aaagttaagt atggtggcaa aaatggtata
     3241 ggaaacttga taaaagttaa cacaaacgca atcgcgctca acatacttat tgtgataaga
     3301 cgtttatttt gttgcatatt caattttctc cttccattca tctcgtaaga ataggaggat
     3361 tattgcacaa caaaaaacct ccatcttctc ccatccagac tatactgtcg gctctagaat
     3421 ctcactagat cagccactaa tatgatacat actagcaggt cgcaggctta atttactgcc
     3481 ggttgggaat tacacccgac cccgaagatg aattttacat tattaaattt aattcccatt
     3541 aattttatac tatatatcac aaaacctcaa taactatgct taaaatgaaa attaattaag
     3601 ttggtgatgt ataatgaatt tgcgatttgc gagcattggt tggaatgttg tttgtttgat
     3661 taatttttga gatggctttt ttgaacctat ctttttattg tttttagttt aatcattcgt
     3721 gttagcttat tcatgattaa atacacctac ttctattaca tccttcttac tataattagg
     3781 tcttgttgta tttatatatt tattagaaat ttgaagagaa acattcaaat catgaggaat
     3841 attctttttc ttttttagca cttcaga
//
LOCUS       DAMZKC010000189         3774 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.192, whole genome shotgun sequence.
ACCESSION   DAMZKC010000189 DAMZKC010000000
VERSION     DAMZKC010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3774
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.192"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..1234
                     /locus_tag="POB55_002354"
     CDS             <1..1234
                     /locus_tag="POB55_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HDI6501295.1"
                     /translation="TMIILVIFSLAFYPAYNPKPKDLPIGILNEDKGTTIQDKNVNIG
                     KKLEDKLLDSDSNKIKWVKVDSEKDLEKDLKDQKIFGVAIIDKDFSKDAMSKTQKVVM
                     DSKKEEMQQKVASGEIPPQVVQQMKQKMGNQQVEVKQAKFKTIVSEGSSLQGSQIASA
                     VLTGMGDNINAQITKQSLETLTSQNVKVNAADINGLTNPVKVDNEKLNKVKDHQAGGN
                     APFLMFMPIWIGSIVTSILLFFAFRTSNNIVVQHRIIASIGQMIFAVVAAFAGSFVYI
                     YFMQGVQGFDFDHPNRIAIFVAFAILGFVGLILGVMVWLGMKSVPIFFILMFFSMQLV
                     TLPKQMLPESYQKYVYDWNPFTHYATSVRELLYLNHHIELNSTMWMFIGFMIFGAVSS
                     LVSAIVRKHSTKRTEVPS"
     gene            1441..1872
                     /locus_tag="POB55_002355"
     CDS             1441..1872
                     /locus_tag="POB55_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2871 domain-containing protein"
                     /protein_id="HDI6501296.1"
                     /translation="MRRLLYSFLFYMIIGLFSGFFYRELTKAYDFTGTTQLSLVHTHT
                     LILGMFMFLILLPLEKLFKLSSYYLFNWFFYVYNIGVIVTIGMMVTKGFFQVTGKSYS
                     PEAFAGFAGIGHTGMLAGLLLLFFLLRQAILKEPRDESFKK"
     gene            1977..2093
                     /locus_tag="POB55_002356"
     CDS             1977..2093
                     /locus_tag="POB55_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501297.1"
                     /translation="MVNYAIYFNNININSIMALNDNNYQFERVNERNEYRES"
     gene            2104..3138
                     /locus_tag="POB55_002357"
     CDS             2104..3138
                     /locus_tag="POB55_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDI6501298.1"
                     /translation="MKDVTIIGGGPSGLYASFYAGLRDMSVRLIDVQSELGGKMRIYP
                     EKIIWDIGGIAPKPCHEILKDTIKQGLYFKPEVHLNERVVDIRKKAERHFEVETEAGE
                     IYTSKAVIIAIGAGIINPKQLDVKGVERYQLTNLHYVVQSYRRFKDKDVLISGGGNTA
                     LDWAHDIAKIAKSVTVVYRKEDVSGHEAMKTLVTDLNVKLCPKTRIKYLVGNDDETHI
                     SEVVLEHVESGDTHTVKFDDVIISHGFDRCNTLLSETSSKLDMHDDCRVKGFGNTTTS
                     IPGIYACGDIVYHDAKSHLIASAFSDGANAANLAKTYIQPDANAEGYVSSHHEVFKEA
                     NKTIVNKHLY"
     gene            3174..3575
                     /locus_tag="POB55_002358"
     CDS             3174..3575
                     /locus_tag="POB55_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6501299.1"
                     /translation="MVKVTYDIPTCEDYCALRINAGMSPKTREAAEKGLPNALFTVTL
                     YDKDWLIGMGRVIGDGGTAFQIVDIAVSKSYQGQGYGSQIMEHIMKYIKNVSVESAYV
                     SLIADYPADKLYTKFGFIPTEPDSGGMYIKF"
BASE COUNT         1298 a          478 c          752 g         1246 t
ORIGIN      
        1 aactatgatt atcttggtaa tcttttcttt agctttttat cctgcatata atcctaaacc
       61 aaaagattta ccaattggta tattaaacga ggataaaggt acaacgattc aagataaaaa
      121 tgttaacatt ggtaaaaaat tagaggataa attattagat agtgattcta ataaaattaa
      181 atgggttaag gttgatagtg aaaaagacct tgaaaaagat ttgaaagatc aaaaaatctt
      241 tggagtagct attattgata aagacttttc aaaagatgct atgagtaaaa cacaaaaagt
      301 agttatggat agtaaaaaag aagaaatgca acaaaaagtt gcttcaggtg aaattccgcc
      361 acaagtggtt caacaaatga aacaaaaaat ggggaatcaa caagtagagg ttaagcaggc
      421 taaatttaaa acgattgtaa gtgaaggatc aagcttacaa ggttcacaaa ttgcatcagc
      481 tgtattaact ggtatgggtg ataatattaa tgctcaaatt acgaagcaaa gtttggaaac
      541 attaacgagt caaaatgtta aagtcaatgc cgcggacatc aatggtttga cgaatccagt
      601 aaaagtggat aatgaaaaac ttaataaagt taaagatcac caagcaggtg gtaatgcacc
      661 attcctaatg tttatgccaa tttggatagg ttcaatcgta acgtctatct tattgttctt
      721 tgcatttaga actagtaaca atatcgtcgt gcaacatcgt atcattgctt caattggaca
      781 gatgatattt gcagttgttg cagcatttgc aggtagcttt gtttatattt atttcatgca
      841 aggcgttcaa ggatttgatt ttgaccatcc aaatcgtatc gcaatttttg tagcatttgc
      901 gattcttggt ttcgtgggcc ttattttagg tgttatggta tggctaggta tgaagtcagt
      961 tccaattttc ttcattttaa tgttctttag tatgcaactt gtaacgttac ctaaacaaat
     1021 gttgcctgaa agttatcaaa aatatgtata tgattggaat ccattcacac actatgcaac
     1081 aagtgtaaga gaactattat acttgaatca tcatattgaa ttaaatagta caatgtggat
     1141 gtttataggg tttatgattt ttggcgctgt atcaagttta gtttcagcta ttgttagaaa
     1201 acatagcaca aaacgtactg aagtgccgtc ataatttaat aaatagaaat aatagctata
     1261 caagacgttt gtagcgagcg tcatggtata gctattttat ttgtatagcg cttgatgtaa
     1321 agttatttta gctgtcatac tatgtgctaa accaaattca gttgtgtcat atcttcgctt
     1381 ttttgtcact gttataatag gtataatcgg atataatgaa aaggtgaggg aggattcaaa
     1441 atgcgtaggt tattatattc atttcttttt tatatgatca taggtttatt tagtggcttt
     1501 ttctatagag agctaacaaa agcatatgat tttactggta caacacaatt atcacttgta
     1561 cacacacata cacttatttt aggtatgttt atgtttttaa tattattacc attggagaag
     1621 ttatttaaat tatcaagtta ctacttattt aactggttct tctatgtata taacataggt
     1681 gttatcgtta ctataggtat gatggtgaca aaaggattct tccaagtcac tggtaaatca
     1741 tactctccag aagcatttgc aggctttgca ggtattgggc acacaggtat gcttgcaggt
     1801 ttattgttgc tatttttcct tttaagacaa gcaattctta aagaaccacg tgatgagtct
     1861 tttaaaaaat aatataacta gaactaaaaa atatacacat ctttcaaagg aacattgttg
     1921 tttcttactt tgaaaggtgt gtttttatat tgtgatggga tagttattga taattcatgg
     1981 ttaactatgc gatttatttt aacaatataa atattaatag tataatggct ttaaatgata
     2041 ataattatca atttgagcgc gttaatgaaa gaaatgaata tagagagtca taggaggcac
     2101 ataatgaaag acgttacaat cattggtggc ggtccgtctg gtttgtatgc aagcttttat
     2161 gctggattgc gagatatgtc tgtaagattg atagatgttc aatctgaatt agggggtaag
     2221 atgagaattt accctgaaaa aattatttgg gatattggcg gcattgcgcc aaaaccttgt
     2281 catgaaattt taaaagatac aattaagcaa ggattatatt ttaaaccgga agttcatttg
     2341 aatgaacgtg tggtagatat taggaaaaag gcagaacgtc atttcgaagt tgaaacagaa
     2401 gcgggagaaa tatatacatc gaaagcagtt atcattgcaa ttggtgcggg tattattaat
     2461 ccaaaacaat tagatgttaa aggtgtagag agatatcaat taactaattt acattatgtt
     2521 gtacaaagtt acagacgttt caaagataaa gatgtattaa tatcaggtgg aggaaataca
     2581 gcattagatt gggcgcatga tattgccaag attgctaaaa gcgtgacagt tgtttatcga
     2641 aaagaagatg taagtggtca tgaagcgatg aaaacgttgg tgacagattt aaatgtgaaa
     2701 ctatgcccaa aaacacgtat taaatacttg gtcggcaatg atgatgaaac gcatataagt
     2761 gaagttgttt tggaacatgt tgaaagtggc gatacgcata cagttaaatt tgacgatgtc
     2821 attattagtc atgggtttga tcgttgtaat acattattga gtgaaacatc ttcaaagcta
     2881 gatatgcatg atgattgtcg tgttaaagga tttggtaata cgacaactag tatacctggt
     2941 atctatgcgt gtggagatat tgtttatcat gatgcaaaat cacatctaat tgcaagtgca
     3001 tttagtgatg gtgctaatgc agcgaacctt gctaaaacat atatccaacc agatgcgaat
     3061 gcagagggtt atgtttcaag tcatcatgaa gtgtttaaag aagcgaataa gactattgta
     3121 aataaacatt tatactaggt tgatatattg tgtgatgaaa agtggggtaa ataatggtta
     3181 aagtgacata tgatattccg acttgcgagg attattgcgc attaaggatt aacgcaggta
     3241 tgagtccaaa gacgcgcgaa gcagctgaaa aaggattacc taatgcctta tttacagtaa
     3301 ccttgtatga taaagattgg ttaattggta tgggtagagt aattggcgat ggcggaactg
     3361 cttttcaaat tgttgatatt gctgtttcga aaagttatca aggtcaaggt tacggcagtc
     3421 aaattatgga acatattatg aagtatatta aaaatgtatc tgtcgaaagt gcatacgtta
     3481 gtctgatcgc agactacccg gcggataaat tatatacaaa atttggattt atacctacgg
     3541 aaccagattc aggtggaatg tatattaagt tttaaaaata aaaagacatc tcgcaaaacg
     3601 ggatgtcttt ttgatgggaa aatatttccc gatttagata gatataccaa tgattagacg
     3661 aagaaaagca tttaattaac tagttttatc taataaatca ttagtgattt tttgtttaaa
     3721 atttttgtat tcagtaaaca attcttcttg agttttgaca ttttgtgagt cagt
//
LOCUS       DAMZKC010000190         3709 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.193, whole genome shotgun sequence.
ACCESSION   DAMZKC010000190 DAMZKC010000000
VERSION     DAMZKC010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3709
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.193"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            450..1574
                     /locus_tag="POB55_002359"
     CDS             450..1574
                     /locus_tag="POB55_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HDI6501300.1"
                     /translation="MKIAIIGAGIGGLTAAALLQEQGHTIKVFEKNESVKEIGAGIGI
                     GDNVLKKLGNHDLAKGIKNAGQILSTMTVLDDKDRLLTTVKLKSNTLNVTLPRQTLID
                     IIKSYVKDDAIFTNHEVTHIDNETDKVTIHFAEQESEAFDLCIGADGIHSKVRQSVNA
                     DSKVLYQGYTCFRGLIDDIDLKHPDCAKEYWGRKGRVGIVPLLNNQAYWFITINSKEN
                     NHKYSSFGKPHLQAYFNHYPNEVREILDKQSETGILLHNIYDLKPLKSFVYGRTILLG
                     DAAHATTPNMGQGAGQAMEDAIVLVNCFNTYDFEKALQRYDKIRVKHTAKVIKRSRKI
                     GKIAQYRSRLVVAVRNRIMKMMPNALAAGQTKFLYKSKEK"
     gene            complement(1655..2608)
                     /locus_tag="POB55_002360"
     CDS             complement(1655..2608)
                     /locus_tag="POB55_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HDI6501301.1"
                     /translation="MEKVYVAGAIPEVGLKLLQEHFEVEMYEGKGLVDKDTLIKGVKN
                     ATALISLLSTNVDKDVIDAGKDLKIIANYGAGFNNIDIEYAREKSIDVTNTPKASTNA
                     TADLTIGLVLAVARRIVEGDQLSRTTGFDGWAPLFFRGREVSGKTIGIIGLGEIGSAV
                     ARRARAFDMDVLYTGPNRKEEKEREIGAKYVDLDTLLKNADFITINAAYNPKMHHLID
                     TEQFKMMKSTAYLINASRGPIVHEQALVQALKDNEIEGAALDVYEFEPDITDDLKSLN
                     NVVLTPHIGNATFEARDMMSKIVANAAISAVQGEKPQFVVN"
     gene            complement(2970..3470)
                     /locus_tag="POB55_002361"
     CDS             complement(2970..3470)
                     /locus_tag="POB55_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDI6501302.1"
                     /translation="MKKLVTATTLTAGIGTALVGQAHHADAAENYTNYNNYNYNTTQT
                     TTTTTTTTTTSSISHSGNLYTAGQCTWYVYDKVGGEIGSTWGNANNWAAAAQGAGFTV
                     NHTPSKGAILQSSEGPFGHVAYVESVNSDGSVTISEMNYSGGPFSVSSRTISASEAGN
                     YNYIHI"
BASE COUNT         1235 a          597 c          658 g         1219 t
ORIGIN      
        1 gcattgtctg tagaaattgg gaatccaatt tctctatgtt ggggccccgc caacttgcac
       61 attattgtaa gctgactttt cgtcagcttc tgtgttggga cccccacccc aactcgcatt
      121 gcctgtagaa tttcttttcg aaattctctg tgttggggcc cctgactaga gttgaaaaaa
      181 gcttgttgta agcgcatttt cattcagtca aatactgcca atataatatt atagacccta
      241 ggacattgat ttatgtccca agctcccaca tcattgttac aaatgtacat gatgtaaatg
      301 aatattgctg tctaaatgtg catgtaatat acaatggtgc agataataca cttaagtcct
      361 taaaaatgaa acgttagttc caagagtcat ttttaaacaa tagtgcatgt gataaaatag
      421 aaaagaatga aaaatataga ggtgacaata tgaagatagc aattataggt gcaggcatcg
      481 gtggattaac agctgctgca ttattacaag aacaaggtca tactattaaa gtctttgaaa
      541 aaaatgagtc agttaaagaa attggcgctg ggattggtat cggagataat gtgcttaaaa
      601 aactaggtaa tcatgactta gctaaaggta ttaaaaatgc tgggcaaatc ttatctacaa
      661 tgacagtgtt agatgacaaa gatcgcctgt taactactgt taaattaaaa agtaatacat
      721 tgaatgtgac gttaccacgt caaacattaa ttgacattat taaatcttat gtaaaagatg
      781 acgcaatatt tacaaatcat gaagtcacgc atatagataa tgagacagat aaagttacca
      841 tacatttcgc ggaacaagaa agtgaagcat ttgatttatg tattggtgct gatggaattc
      901 attctaaagt gagacaatct gtaaatgctg acagtaaagt attatatcaa gggtatacat
      961 gctttagagg tttaattgat gatattgatt taaagcatcc ggattgtgca aaagaatact
     1021 ggggaagaaa aggaagagta ggtattgttc cgttattaaa taatcaagca tattggttca
     1081 ttacaattaa ctcgaaggaa aacaatcata aatatagttc gtttggtaaa cctcatttgc
     1141 aagcatactt taatcactat ccaaatgaag ttagagagat cttagacaaa caaagtgaaa
     1201 caggtatctt attgcataat atttatgatt tgaaaccact caaatctttt gtttatggtc
     1261 gtactatttt actaggagat gcagcacatg cgacaacgcc taatatgggg caaggtgctg
     1321 gacaagcaat ggaagatgct atcgtattag taaattgttt taacacatac gactttgaaa
     1381 aagcattaca gcgttatgat aaaatacgtg tcaaacatac tgcaaaagta attaagcgtt
     1441 ctagaaaaat cggtaaaatt gcccaatatc gtagtcgttt agttgttgca gttagaaatc
     1501 gtattatgaa aatgatgcca aatgcattag cagctggaca aactaaattc ttatataaat
     1561 cgaaagagaa ataatacaac aatatgaaaa cccccgtatg ttgaaacgag agctcaacat
     1621 atgggggttc ttgtttttat aataaattca attattagtt aacgacaaat tgtggtttct
     1681 caccttgaac ggcactaatt gcagcattag caacaatttt agacatcatg tcacgtgctt
     1741 caaatgtagc attaccaata tgcggtgtta atactacatt attaagtgat tttaagtcat
     1801 cggtaatatc tggttcaaat tcatatacat caagtgcagc accttcaatt tcattatctt
     1861 tcaatgcttg cactagtgct tgttcgtgca cgattggacc acgagaggca ttgattaaat
     1921 acgccgtaga tttcatcatt ttaaattgtt ctgtatcaat taaatgatgc attttaggat
     1981 tataagcagc gttgatagtg ataaaatctg cattctttaa tagtgtatct aaatctacat
     2041 attttgcacc gatttctcgt tctttttctt ctttgcgatt aggtcctgtg tatagtacat
     2101 ccatgtcaaa tgctcttgca cgacgcgcta ctgcactacc aatttcacct aaaccgataa
     2161 tgccgattgt tttcccagat acttctctac ctctgaaaaa taaaggtgcc catccatcaa
     2221 atccagttgt acgtgataat tggtcccctt caacaatacg acgcgctact gcaagtacta
     2281 atccaattgt taaatcggca gtcgcgtttg ttgatgcttt aggtgtgttt gtaacatcta
     2341 tacttttttc tcgggcatac tcgatatcaa tattattaaa accagcgcca tagttggcaa
     2401 tgatttttaa gtctttacca gcatcgataa catctttatc aacgtttgta gataataaac
     2461 taattaaggc agtcgcgttt ttaacacctt taattaaagt gtctttatcg actaatcctt
     2521 taccttcata catttcaact tcaaaatgtt cttgtaaaag ttttaaacct acttctggta
     2581 ttgcaccagc aacataaact ttttccataa aagatcactc cttttatctt agtatagtag
     2641 aagattagac agtatacaac tatgtcatga tgtcttgtgt atcaatgatg taagcgcgta
     2701 cttttgatgg aggtgatata acttaggcac tgtagaacta tgaatattgt aatgtggaaa
     2761 aactggatca attaaattag ataacgtagt tttaaagtta atagtattag aaaaaattaa
     2821 tattttgaat atgggaggaa atataaataa gtaggtggca acgaaaaata gcaaaaaaag
     2881 agcttctcct ataaaggaaa gctcaaagtt ttttgatgac atatgtacta gaattaagtt
     2941 tcaagacaat atgtatcatc gtgtttatat taaatatgga tgtagttgta gttacctgct
     3001 tcacttgcag aaatagttct agaacttact gagaaaggtc cgccactata attcatttct
     3061 gaaattgtaa ctgaaccatc actgtttaca ctttctacat atgcaacgtg accaaatggt
     3121 ccttcagaag attgtaggat agcgccttta gaaggtgtat gatttactgt gaatccagca
     3181 ccttgtgcag cagcagccca attattagca tttccccaag tagaaccgat ttctccgcct
     3241 actttatcat atacatacca agtacattgt cctgcagtgt ataagttacc agaatgtgaa
     3301 attgatgatg tagttgtcgt agttgttgta gtcgttgtag tttgagtcgt gttgtagtta
     3361 tagttgttgt aatttgtata attttcagca gcatctgcat gatgtgcttg acctactaat
     3421 gctgtgccga ttcctgctgt taacgtagtt gctgttacta attttttcat gaataaagtc
     3481 ctccaaagtt ctatatcttt ttttataaat aaaacgtagc gactgtttta ttctcacatc
     3541 tcgaattgat gacaatagtt actttaacaa aattaatgct tcttgtgggg aatgttattg
     3601 atttgtaaaa gaataaaaaa actttgacta attttgtaat aaaaattagt caaagttaca
     3661 atgagattaa cagataatta ataggaaata tttatttgta atatgttta
//
LOCUS       DAMZKC010000191         3694 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.194, whole genome shotgun sequence.
ACCESSION   DAMZKC010000191 DAMZKC010000000
VERSION     DAMZKC010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3694
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.194"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            104..259
                     /locus_tag="POB55_002362"
     CDS             104..259
                     /locus_tag="POB55_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501303.1"
                     /translation="MMVKKLLKAIKQNFLYEQSNITFKMIVFGYKRLSKMLFQFEINH
                     INFLWEG"
     gene            267..788
                     /locus_tag="POB55_002363"
     CDS             267..788
                     /locus_tag="POB55_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501304.1"
                     /translation="MINIISAIGSIGTFIMALFYFVSVSVQLYQMKISFLPALGFNQI
                     LLEREEDQLNIMNSATEEHHHKDYIKLYNLGGGAAKKIAIEVLLGNDKVIQKKYVNIL
                     PSKEGYMLPINKNVYEELERTIENNGYEADLNVRMTYYHNVSRKQQEVILKGQIDCFN
                     TYNNKEIYDLQFI"
     gene            927..1457
                     /locus_tag="POB55_002364"
     CDS             927..1457
                     /locus_tag="POB55_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDI6501305.1"
                     /translation="MQIRQIHQHDFAQVDQLIRTAFENSEHGYGNESELVDQIRLSDT
                     YDNNLELVAVLQNEVVGHGLLSEVYLDNEAQREIGLVLAPVSVDIHHQNKGIGKRLIQ
                     ALEREAILKGYNFISVLGWPTYYANLGYQRASMYDIYPPYDGIPDEAFLIKELKVNSL
                     AGKTGTINYTSAFEKI"
     gene            1720..>3694
                     /gene="clfA"
                     /locus_tag="POB55_002365"
     CDS             1720..>3694
                     /gene="clfA"
                     /locus_tag="POB55_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin clumping factor ClfA"
                     /protein_id="HDI6501306.1"
                     /translation="MNMKKKEKHAIRKKSIGVASVLVGTLIGFGLLSSKEADASENSV
                     TQSDSASNESKSNDSSSVSAAPKTDDTNVSDTKTSSNTNNGETSVAQNPAQQETTQSA
                     LTNATTEETPVTGEATTATNQANTPATTQSSNTNAEELVNQTSNETTSNDTNTVSSVN
                     SPQNSTNAENVSTTQDTSTEATPSNNESAPQSTDASNKDVVNQAVNTSAPRMRAFSLS
                     AVAADAPAAGKDITNQLTNVTVGIDSGDTVYPHQAGYVKLNYGFSVPNSAVKGDTFKI
                     TVPKELNLNGVTSTAKVPPIMAGDQVLANGVIDSDGNVIYTFTDYVDTKENVTANITM
                     PAYIDPENVTKTGNVTLTTGIGSTTANKTVLVDYEKYGKFYNLSIKGTIDQIDKTNNT
                     YRQTIYVNPSGDNVIAPVLTGNLKPNTDSNALIDQQNTSIKVYKVDNAADLSESYFVN
                     PENFEDVTNSVNITFPNPNQYKVEFNTPDDQITTPYIVVVNGHIDPNSKGDLALRSTL
                     YGYDSRFVWRSMSWDNEVAFNNGSGSGDGIDKPVVPEQPDEPGEIEPIPEDSDSDPGS
                     DSGSDSNSDSGSDSGSDSTSDSGSDSASDSDSASDSDSASDSDSASDSDSASDSDSAS
                     DSDSASDSDSASDSDSASDSDSDNDSDSDSDSDS"
BASE COUNT         1369 a          526 c          708 g         1091 t
ORIGIN      
        1 aagtgttatt aatttggaaa atactggtca aagatgggag ctctcaaaag cgttattgta
       61 ttttttagtc aatacaaata gattgccgta ataataatcg tacttgatgg ttaaaaaatt
      121 acttaaggct ataaagcaaa actttttata tgagcagtcg aatataacgt ttaaaatgat
      181 tgtttttgga tataaacgat taagtaaaat gctttttcag tttgaaatta atcatataaa
      241 tttcttatgg gaaggttgat atcttaatga ttaatattat ttcagctata ggatctattg
      301 gaacatttat tatggcttta ttttattttg tatcagtttc agttcaactt tatcaaatga
      361 aaattagctt tctgccagct ttaggtttta accaaatttt attagaaagg gaggaggatc
      421 aacttaatat aatgaattcg gcaacagaag agcatcatca taaagattat attaaactat
      481 ataatttagg tggcggtgct gctaaaaaaa ttgcaataga ggttttattg ggtaatgata
      541 aagtcattca gaaaaaatac gtgaatattt tacctagtaa agaagggtac atgttaccaa
      601 ttaataaaaa tgtgtacgaa gaattagaaa gaacgattga gaacaatggt tatgaagctg
      661 atttgaatgt acgtatgact tattatcata atgtaagtcg caaacaacag gaagttatat
      721 taaaaggtca aatcgactgt tttaatactt ataataataa agaaatttat gatttgcagt
      781 ttatctaaaa attgatttaa gagggtagtt gtttattgcg aaaaatatca ttcaatttta
      841 atgaaataat ggcgtcatta ctataaaata ttactttatg ttgtaatgca tttttctata
      901 agatagaact aaaaggaggg gcaaagatgc aaattagaca aatacatcaa catgactttg
      961 ctcaagttga ccagttaatt agaacggcat ttgaaaatag tgaacatggt tatggtaatg
     1021 aatcagaact agtagaccaa attcgtctaa gtgatacgta tgacaataac ttagaattag
     1081 tagctgttct tcaaaatgaa gttgtagggc acggtttact aagtgaagtt tatcttgata
     1141 acgaggcaca acgggaaatt ggattagtgt tagcacctgt atctgttgat attcatcatc
     1201 aaaataaagg tattgggaag cgattgattc aagcattaga acgagaagca atattaaaag
     1261 gatataattt tatcagtgta ttaggatggc cgacgtatta tgccaatcta ggatatcaac
     1321 gcgcaagtat gtacgacatt tatccaccat atgatggtat accagacgaa gcgtttttaa
     1381 ttaaagaatt aaaagtgaac agtttagcgg gaaaaacagg taccataaat tacacatctg
     1441 cttttgaaaa aatatgattt caagctagga ttacattagg tagagttcat attaataata
     1501 aaaaatgttt gcaatgaaat cgtgcattgt cgtttgcaat ttttaaaata gcaataaata
     1561 aaatgtttgt tagtaaagta ttattgtgga taataaaata tcgatacaaa ttaattgcta
     1621 taatgcaatt ttagtgtata attccattaa cagagattaa atatatcttt aaagggtata
     1681 tagttaatat aaaatgactt tttaaaaaga gggaataaaa tgaatatgaa gaaaaaagaa
     1741 aaacacgcaa ttcggaaaaa atcgattggc gtggcttcag tgcttgtagg tacgttaatc
     1801 ggttttggac tactcagcag taaagaagca gatgcaagtg aaaatagtgt tacgcaatct
     1861 gatagcgcaa gtaacgaaag caaaagtaat gattcaagta gcgttagtgc tgcacctaaa
     1921 acagacgaca caaacgtgag tgatactaaa acatcgtcaa acactaataa tggcgaaacg
     1981 agtgtggcgc aaaatccagc acaacaggaa acgacacaat cagcattaac aaatgcaact
     2041 acggaagaaa ctccggtaac tggtgaagct actacggcaa cgaatcaagc taatacaccg
     2101 gcaacaactc aatcaagcaa tacaaatgcg gaggaattag tgaatcaaac aagtaatgaa
     2161 acgacttcta atgatactaa tacagtatca tctgtaaatt cacctcaaaa ttctacaaat
     2221 gcggaaaatg tttcaacaac gcaagatact tcaactgaag caacaccttc aaacaatgaa
     2281 tcagctccac agagtacaga tgcaagtaat aaagatgtag ttaatcaagc ggttaataca
     2341 agtgcgccta gaatgagagc atttagttta tcggcagtag ctgcagatgc accggcagct
     2401 ggcaaagata ttacgaatca gttgacgaat gtgacagttg gtattgactc tggagataca
     2461 gtttatccgc accaagcagg ctatgtcaaa ctgaattatg gtttttcagt gcctaattct
     2521 gctgttaaag gtgacacatt caaaataact gtacctaaag aattaaactt aaatggtgta
     2581 acttcaactg ctaaagtgcc tccaattatg gccggagatc aagtattggc aaatggtgta
     2641 atcgatagtg atggtaatgt tatttataca tttacagact atgttgatac taaagaaaat
     2701 gtaacagcta atattactat gccagcttat attgaccctg aaaatgttac aaagacaggt
     2761 aatgtaacat tgacaactgg cataggtagt acaacagcaa acaaaacagt attagtagat
     2821 tatgaaaaat atggtaagtt ttataactta tctattaaag gtacaattga ccaaatcgat
     2881 aaaacaaata atacgtatcg tcagacaatt tatgtcaatc caagtggaga taatgttatt
     2941 gcgccggttt taacaggtaa tttaaaacca aatacggata gtaatgcatt aatagatcag
     3001 caaaatacaa gtattaaagt atataaagta gataatgcag ctgatttatc tgaaagttac
     3061 tttgtgaatc cagaaaactt tgaggatgtc actaatagtg tgaatattac attcccaaat
     3121 ccaaatcaat ataaagtaga gtttaatacg cctgatgatc aaattacaac accgtatatt
     3181 gtagttgtta atggtcatat tgatccgaat agcaaaggtg atttagcttt acgttcaact
     3241 ttatatggat atgactcaag gtttgtatgg agatctatgt catgggacaa cgaagtagca
     3301 tttaataacg gatcaggttc tggtgacggt atcgataaac cagttgttcc tgaacaacct
     3361 gatgagcctg gtgaaattga accaattcca gaggattcag attctgaccc aggttcagat
     3421 tctggcagcg attctaattc agatagcggt tcagattcgg gtagtgattc tacatcagat
     3481 agtggttcag attcagcgag tgattcagat tcagcgagtg attcagattc agcaagtgat
     3541 tcagactcag cgagtgattc agattcagca agcgattcag attcagcgag cgattcagat
     3601 tcagcgagtg attcagattc agcgagtgat tcagattcag caagcgattc cgactcagac
     3661 aatgactcgg attcagatag cgattctgac tcag
//
LOCUS       DAMZKC010000192         3688 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.195, whole genome shotgun sequence.
ACCESSION   DAMZKC010000192 DAMZKC010000000
VERSION     DAMZKC010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3688
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.195"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(182..1048)
                     /locus_tag="POB55_002366"
     CDS             complement(182..1048)
                     /locus_tag="POB55_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HDI6501307.1"
                     /translation="MNEQWLEHLPLKDIKEISPVSGGDVNEAYRVETDTDTFFLLVQR
                     GRKESFYAAEIAGLNEFERAGITAPRVIASGEVNGDAYLVMTYLEEGASGSQRQLGQL
                     VAQLHSQQQEEGKFGFSLPYEGGDISFDNHWQDDWCTIFVDKRLDHLKDELLNRGLWD
                     ANDIKVYDKVRRQIVAELEKHQSKPSLLHGDLWGGNYMFLKDGRPALFDPAPLYGDRE
                     FDIGITTVFGGFTSEFYDAYNKHYPLAKGASYRLEFYRLYLLMVHLLKFGEMYRDSVA
                     HSMNKILQDTTS"
     gene            1266..2330
                     /locus_tag="POB55_002367"
     CDS             1266..2330
                     /locus_tag="POB55_002367"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HDI6501308.1"
                     /translation="MYKLIKPFLFKIEPEKAHGLTIDALKTLQKFPVLFPVVDKLFTY
                     KNPTLSQTIQGNTYDNPIGLAAGFDKSCEVPKALEHLGFGALELGGITPKPQPGNPQP
                     RMFRLLEDDALINRMGFNNIGMNKALSHLRKNAYQVPVGINVGVNKMTPYEARYQDYI
                     KVIDTFKHDVSFFTVNISSPNTENLQNFHDKDEFSMLCQALTTFKKQHDVTVPIYLKL
                     TSDMDFDGLKALLPAITETFDGIILANTTRQRDGLTSANKVEEGGLSGRPLFERNLKL
                     IKYAYQQTNGEFLIIGTGGVFSTEDAIKMMRHGASLIQIYSSLVIEGPGLTKKMNKGI
                     ARYLKDHHFDNVSDIIGLDA"
     gene            2485..2760
                     /locus_tag="POB55_002368"
     CDS             2485..2760
                     /locus_tag="POB55_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501309.1"
                     /translation="MTKETDNKKTVATSDNKDTQQQNQPEEDERQGLNGYRKTDLDLE
                     IEQELREMMKTGENETKNDYKKFKVFSLISTLVIVILAIIRFVHKMM"
     gene            complement(2946..3668)
                     /locus_tag="POB55_002369"
     CDS             complement(2946..3668)
                     /locus_tag="POB55_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HDI6501310.1"
                     /translation="MINAEPIISKMKNQKINYDKVLKKLIGQWEREAIRPKILLHSCC
                     APCSTYTLEFLTQYADIAIYFANSNIHPKNEYLRRAKVQEQFVEDFNRKTGANVKYIE
                     APYEPHKFVKMVKDKELADEKEGGLRCTACFEMRLDIVAKAAVEHGYDYFGSAITLSP
                     KKNAQLINELGMDVQKIYDVNYLPSDFKKSKGYERSIEMCNDYNIFRQCYCGCVFAAM
                     QQGIDFKTVNKEAKAFLEQYPD"
BASE COUNT         1245 a          691 c          543 g         1209 t
ORIGIN      
        1 aaaagttata aaataacttt tattgcaact caaccttgtt ttacattaca gaaattaaca
       61 atagcgatta aaattttatt ttccaacaat aaaaaagcac aaatattttt attgaggcag
      121 ctgtatcgct tctcattgta aaaatatctg tgcatattat tatctcaatc taacgtaata
      181 actaacttgt tgtatcttgt aaaatcttat tcatagaatg cgcaacacta tcacggtaca
      241 tttcaccaaa tttcaataaa tggaccatca ataaatataa acgataaaat tcaagtctat
      301 aggatgcacc ttttgcgagt ggataatgtt tattatacgc atcataaaat tcgctcgtaa
      361 aaccaccaaa taccgttgta ataccgatat cgaattctct gtcaccatat aatggtgctg
      421 gatcaaataa tgcaggacgt ccatctttta aaaacatata attaccaccc cataggtcac
      481 catgtaataa agacggttta ctttgatgct tttctaattc cgccacaatt tgacgtcgca
      541 ctttgtcata tactttgata tcgttggcat cccatagccc tctatttagc aactcatctt
      601 tcaaatggtc caagcgcttg tcgacaaaaa tggtacacca atcgtcttgc caatgattat
      661 caaaagaaat atcgccacct tcataaggta atgagaagcc aaatttgcct tcttcttgtt
      721 gctgactgtg taattgagct acgagttgcc ctaattggcg ttgactccct gaagcccctt
      781 cttctaaata cgtcatcact aaatacgcat caccgttaac ctcgccactt gcaattactc
      841 taggtgccgt gatacctgca cgttcaaatt catttaaacc tgcaatttct gcagcataaa
      901 atgattcttt acgtccacgt tggacaagta agaaaaatgt atccgtatct gtttcgactc
      961 gatatgcttc gtttacatca ccaccactca ctggtgaaat ctctttaata tcttttaaag
     1021 gtaaatgctc taaccattgt tcattcatac aaacaccttc caatctactt actttatact
     1081 ataatattgc ctaattagga tgatttaatc ttaaaaacgt tattcaaact tgcgtcaatt
     1141 ttcaaaactt tttagtttta actaggaaat tatgtaggga aatgctagtc tattaaaaga
     1201 atatatttca catatattaa gttgaggata gtaagcctca aatataatga aataggagca
     1261 tactcatgta caaattaatt aaacctttct tattcaaaat cgaacccgaa aaagcacacg
     1321 gactaactat cgatgcatta aaaacgttac aaaagtttcc ggttttattc ccagtcgtcg
     1381 ataaactatt tacttataag aatccaacgt tatcacaaac gatacaaggt aatacgtacg
     1441 acaatccaat tggcttagca gctggtttcg acaaatcttg cgaagtacca aaagcattgg
     1501 aacaccttgg attcggtgct ttagaattag gtggtatcac acctaaacct caaccgggta
     1561 accctcaacc acgcatgttt agattattag aagatgatgc cttgataaat cgaatgggct
     1621 tcaataatat tggtatgaac aaagcactca gtcatttgcg taaaaatgct tatcaagtac
     1681 ctgttggtat caatgttggt gtgaataaaa tgacacctta tgaagcgcgt tatcaagatt
     1741 atataaaagt cattgatacg tttaaacacg acgtttcatt tttcacagtc aatatcagtt
     1801 ctccaaatac tgaaaacctt caaaatttcc atgataaaga tgaattttca atgttatgtc
     1861 aagctttgac aacatttaaa aaacaacatg atgtaacagt gccaatttat ttaaaactaa
     1921 cgtctgatat ggatttcgat ggcttaaaag cactattacc agcgattact gagacatttg
     1981 acggtatcat cttagcaaac acaacgcgac aacgagatgg tttaacttct gctaataaag
     2041 tcgaagaagg cggtttgagt ggtcgtccat tatttgaacg taatttaaaa ttgattaagt
     2101 atgcttatca gcaaacaaat ggtgaatttt taattatagg tacaggcggc gtattcagta
     2161 ctgaagatgc aatcaaaatg atgcgtcacg gtgcgtcact tattcaaatt tattcatcac
     2221 ttgttattga aggcccaggt ttaactaaga aaatgaacaa aggcatcgca cgttacttaa
     2281 aagatcatca ttttgacaat gtcagtgata ttataggact agatgcctaa tccaacatag
     2341 tcaaataata ctttaaaact tgctgaatcg tcttattaaa attatgatat aactcaagaa
     2401 gatatattta tcaccatttt tcaaaataat ggcttaaata tcacatatca ttaaatatag
     2461 cgttttataa cggaggcgtt tgttatgact aaagaaacag ataataaaaa aacagtagcg
     2521 acttctgaca acaaagacac acagcaacaa aatcaacctg aagaagatga acgtcaaggt
     2581 ttgaatggtt atcgtaaaac agatcttgat ttagaaatcg agcaagagct acgtgaaatg
     2641 atgaaaacag gagaaaatga aacgaaaaat gactataaaa agtttaaagt gttttcactt
     2701 atttcaacac ttgtcattgt cattttagca attataagat ttgttcataa aatgatgtaa
     2761 ttagagtgag acattgtttt atgtctcagg atccagttat tcattatatc tacaatattt
     2821 acgattatat aaataacccg agattttagt atgattcatt tcactaaaat ctcgggtttc
     2881 tatttgataa tttttaatgg gatatggcat gtatacgttc ttgcctttta tctcatttcc
     2941 aatgattaat ctggatattg ttctaaaaat gctttcgctt ctttattaac tgttttaaaa
     3001 tcaatacctt gttgcatcgc tgcaaagaca catccacaat aacactgcct aaagatatta
     3061 tagtcattac acatttctat ggaacgctca taacctttac ttttcttaaa atcacttggc
     3121 aaatagttca catcgtatat tttttggaca tccataccaa gttcattgat taattgtgcg
     3181 ttctttttag gtgataatgt gattgcactg ccaaaataat catagccatg ttctacagct
     3241 gctttcgcta caatatccaa acgcatttca aaacaagcgg tacaacgtaa gccgccttct
     3301 ttttcatcag ctaattcttt atccttcacc attttcacaa acttatgcgg ttcataaggt
     3361 gcttcaatat acttcacatt cgcaccagtc ttgcgattaa aatcttccac aaattgttct
     3421 tgcactttag cacgtcgtaa gtactcattt ttcggatgaa tatttgaatt cgcgaaataa
     3481 attgcaatgt ctgcatattg tgttaaaaac tctaatgtat atgtactaca aggtgcacaa
     3541 caactatgca ataagatttt aggtctgatt gcttctcttt cccactgccc gattaatttc
     3601 ttcaacacct tgtcataatt aattttttga tttttcattt tactgataat aggttcagca
     3661 ttaatcatga tttaacctcc cacattta
//
LOCUS       DAMZKC010000193         3670 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.196, whole genome shotgun sequence.
ACCESSION   DAMZKC010000193 DAMZKC010000000
VERSION     DAMZKC010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3670
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.196"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..849
                     /locus_tag="POB55_002370"
     CDS             <1..849
                     /locus_tag="POB55_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI6501311.1"
                     /translation="TFVILAKTLNYRLASEEINLTIPSIHKQIKNLEQHLNVKLFETY
                     KNQIILTEDGHTFLPIAQSFIEQYESGIKHIQLKKTMFQSKLNVVVSSYIATFIMPKF
                     LKSFFNEHPFIDVSLHVKNENIEKDINNHTYDIGISRNQPKLREVHSEKVCEGKIVLI
                     APNKENNHLLTEASLFEKYKIISDNHPEYWSSLKNNILNIYEKAQFLSINDVHTSIKL
                     IEMNQGISFLPLYITTNSDYNISVINTKILQAPISFTYIYSKKESPEILVFIKSFKKY
                     IANEQL"
     gene            complement(908..3517)
                     /gene="clpB"
                     /locus_tag="POB55_002371"
     CDS             complement(908..3517)
                     /gene="clpB"
                     /locus_tag="POB55_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HDI6501312.1"
                     /translation="MDINKMTYAVQSALQQAVELSQQHKLQNIEIEAILSAALNESES
                     LYKSILERANIEVDQLNKAYEDKLNTYASVEGDNIQYGQYISQQANQLITKAESYMKE
                     YEDEYISMEHILRSAMDIDQTTKHYINNKVEVIKEIIKKVRGGNHVTSQNPEVNYEAL
                     AKYGRDLVEEVRQGKMDPVIGRDEEIRNTIRILSRKTKNNPVLIGEPGVGKTAIVEGL
                     AQRIVKKDVPESLLDKTVFELDLSALVAGAKYRGEFEERLKAVLKEVKESDGRIILFI
                     DEIHMLVGAGKTDGAMDAGNMLKPMLARGELHCIGATTLNEYREYIEKDSALERRFQK
                     VAVSEPDVEDTISILRGLKERYEVYHGVRIQDRALVAAAELSDRYITDRFLPDKAIDL
                     VDQACATIRTEMGSNPTELDQVNRRVMQLEIEESALKNESDNASKQRLQELQEELANE
                     KEKQAALQSRVESEKEKIANLQEKRAQLDESRQALEDAQTNNNLEKAAELQYGTIPQL
                     EKELRELEDNFQDEQGEDTDRMIREVVTDEEIGDIVSQWTGIPVSKLVETEREKLLHL
                     SDILHKRVVGQDKAVDLVSDAVVRARAGIKDPNRPIGSFLFLGPTGVGKTELAKSLAA
                     SLFDSEKHMIRIDMSEYMEKHAVSRLIGAPPGYIGHDEGGQLTEAVRRNPYSVILLDE
                     VEKAHTDVFNVLLQILDEGRLTDSKGRSVDFKNTIIIMTSNIGSQVLLENVKETGEIT
                     ESTEKAVMTSLNAYFKPEILNRMDDIVLFKPLSIDDMSMIVDKILTQLNIRLLEQRIS
                     IEVSDDAKAWLGQEAYEPQYGARPLKRFVQRQIETPLARMMIKEGFPEGTTIKVNLNS
                     DNNLTFNVEKIHE"
BASE COUNT         1127 a          688 c          477 g         1378 t
ORIGIN      
        1 acatttgtca ttttagcgaa gacgttaaac tatcgattag ctagtgaaga aattaatctc
       61 actatacctt ctatacataa gcaaattaaa aatttagaac agcatctcaa tgtgaaacta
      121 tttgaaactt ataaaaatca aattatcttg actgaagatg gtcatacatt tcttccaatt
      181 gcgcaaagtt ttattgaaca atatgaaagt ggcatcaaac atatccaact taaaaaaacg
      241 atgtttcaat cgaaattaaa tgttgtcgtg tcttcatata tcgcgacatt cattatgccc
      301 aaatttttaa aatctttctt taacgagcat ccttttatcg atgtttctct tcatgtcaaa
      361 aatgaaaata ttgaaaaaga tattaataat catacgtatg acatcgggat tagcagaaat
      421 caacctaaac taagagaagt tcattctgaa aaagtatgcg aaggtaaaat tgtattgatt
      481 gctcccaata aagagaataa tcatcttcta actgaggcat ctttatttga aaaatacaaa
      541 ataataagtg ataaccatcc agaatattgg tcatcactta aaaataatat tttaaatatt
      601 tatgaaaaag cacaatttct atctatcaat gatgtgcata cttcaattaa attaatcgaa
      661 atgaatcaag gtatatcatt cctgcctctt tatataacaa caaatagtga ttacaatatt
      721 tcggttatta acacgaaaat tttacaagca cctatttcat ttacatatat atacagcaaa
      781 aaagaaagcc cagaaatatt ggtgtttatt aaatcattta aaaagtatat tgccaatgaa
      841 caattataat aaatttcaaa tctaaaaaac caagaatgcg attaatcatc acattcttgg
      901 ttcaatttta ttcatgaatt ttttcaacat taaacgttaa gttattgtct gaatttaaat
      961 taactttaat cgttgtacct tctgggaatc cctctttaat catcatacgt gctaatggtg
     1021 tttcaatttg gcgttgtaca aaacgtttta atggtcttgc accgtattga ggttcataag
     1081 cttcttgacc tagccaagct ttagcatcat cagaaacttc aattgagatt cgttgttcta
     1141 ataatcttat atttaattgc gttaagattt tatctacaat catactcatg tcatcaatag
     1201 ataatggttt aaataatacg atatcatcca tacgattcaa aatttctggt ttgaaatatg
     1261 catttaaact tgtcataaca gctttttctg ttgattctgt aatttcacca gtctctttta
     1321 cgttttctaa taaaacttga gatccaatat tacttgtcat aataataata gtatttttaa
     1381 aatcaacgct acgtccttta gaatcagtta aacggccttc atctaaaatt tgcaataata
     1441 cattaaagac gtcagtatgc gctttttcaa cctcatctaa taaaataact gagtatggat
     1501 tacgacgaac cgcttcagtt aattgacccc cttcatcatg tccaatatat cctggaggtg
     1561 cccctatcaa tcttgatact gcatgttttt ccatatattc actcatatca atacgaatca
     1621 tatgtttttc agaatcaaat aatgatgcag ctaatgattt agctaattca gttttaccta
     1681 ctccagttgg acctaggaat aagaaactac caataggtct gtttggatct ttaatacctg
     1741 ctcttgctct aactactgca tctgaaacca ggtcaaccgc tttatcttga cctacaacac
     1801 gtttatgcaa gatgtcactt aagtgaagta atttttcacg ttctgtttca actaattttg
     1861 aaactggtat gcctgtccat tggctgacaa tatcgccaat ttcttcgtct gttacaactt
     1921 cacgaatcat tcgatctgta tcttcacctt gctcatcttg gaaattatcc tctaattctc
     1981 taagttcttt ttccaattga ggaattgttc catattgtag ttcagcagct ttttctaaat
     2041 tgttatttgt ttgtgcatct tccaacgctt gtctactttc atctagttgc gcacgttttt
     2101 cttgtaaatt tgctattttt tctttttctg attctacacg agattgaagt gctgcttgtt
     2161 tctctttttc attggcaagc tcttcttgta gttcttgtaa tctctgtttg ctcgcattgt
     2221 cagattcatt tttaagtgcg ctttcttcaa tttctaattg catgacacgt ctattaactt
     2281 gatccaattc agttggattt gatcccattt ccgtacgaat tgttgcacat gcttggtcaa
     2341 ctaaatcaat cgctttatct ggtaaaaaac gatcagtgat gtaacgatca gacaattcag
     2401 cggcagcaac taaggctcta tcttgaatac gcacaccatg atacacttca tatcgttctt
     2461 ttaaaccacg taaaattgaa attgtatctt caacatcagg ctcactaact gctacttttt
     2521 ggaaacgacg ctctaatgcc gaatcttttt caatatattc tcgatattca tttaaagttg
     2581 ttgcaccaat acaatgtaac tctcctcgtg ctaacattgg ttttagcatg ttgcctgcat
     2641 ccatggcacc atctgtttta ccagcaccta caagcatatg gatttcatca ataaataata
     2701 taattctacc atcagactct ttaacttctt ttaggactgc ttttaatctc tcttcaaatt
     2761 caccacgata tttagcgccc gctactaatg cgcttaaatc taactcaaaa acagttttat
     2821 ctaataatga ttctggcaca tctttcttaa ctatacgttg cgctaatcct tcaacaattg
     2881 cagttttacc aacacctggt tcaccaatga gcacagggtt gtttttagtt ttacgactta
     2941 aaatacgaat cgtatttcga atttcttcat ctcttcctat aacaggatcc attttacctt
     3001 gtctaacttc ttctactaag tcgcggccat atttagctaa tgcttcgtaa ttaacttctg
     3061 gattttgtga tgtcacgtga tttccccctc ttactttttt aataatttct ttgataactt
     3121 ctactttatt atttatataa tgttttgttg tttgatcaat gtccattgcc gaacgtaaaa
     3181 tatgctccat tgaaatatat tcatcttcat attctttcat gtatgattca gccttagtta
     3241 tcaattggtt tgcttgttgg ctaatatatt gaccatattg tatattgtca ccttctacag
     3301 atgcatacgt gtttagtttg tcttcataag ctttgtttaa ttgatctacc tcaatatttg
     3361 ctcgttctaa aatactttta tataagcttt cactttcatt taaggcagcg cttaaaattg
     3421 cctcaatttc tatattttgt aatttatgtt gctgactcag ttcaactgct tgttgtaaag
     3481 cactttgaac agcatatgtc attttattta tatccatata atttcacctc taatttgtag
     3541 agttggtcaa gttgcacttt tgactttgac tttctttgac cttaattata ttatagtccc
     3601 aatataatca tttatcaact cttttacact taaatttcct aatatttgta aatatttttt
     3661 tgaataaaaa
//
LOCUS       DAMZKC010000194         3663 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.197, whole genome shotgun sequence.
ACCESSION   DAMZKC010000194 DAMZKC010000000
VERSION     DAMZKC010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.197"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            126..1550
                     /locus_tag="POB55_002372"
     CDS             126..1550
                     /locus_tag="POB55_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HDI6501313.1"
                     /translation="MLSVKKATKFAVANLIIVSTVFSSVTPAIAQSDKSSKDESFAVG
                     NTKNPKNVIFLVGDGMGPSFNTAYRYYKNDPHAKEMTPTAFDSYLKGTNRTYSNDPKQ
                     NITDSAAGGTAFSSGHKTYNGAIGVDSNKQKVKTVLERAKEKGKSTGLVSTAELTDAT
                     PAAYAAHVTSRDDKNEIAKQFYKDKINGKHKVDVLLGGGAKYFGKSNGNLDKKFKNDG
                     YDLATNSKELSKSDKDKVLGLFADKNMPLAIDASKDEPSLADMQQSALSKLERNKKGF
                     FLMVEGASIDKSAHSNDITGVMSEMEGFEKAFDDAIQYAKKHKDTLVVATADHSTGGL
                     TIGKDKGYEWNPQPIKSMKHSGSYMTEKMAKGEDPEKVINEGYGFEFSQDDMKKVKKE
                     NKKLKRLLKKEKDEKSAKVETQTKALQHAIQKPVNDKSYTGWTSDGHTGEDVNTYAYG
                     PRSESFSGNIENTESAKIIFDIFK"
     gene            1620..1730
                     /locus_tag="POB55_002373"
     CDS             1620..1730
                     /locus_tag="POB55_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501314.1"
                     /translation="MLGAWLTRSWYFLNAKYKPPTNVKITANFFIIQTPF"
     gene            complement(1833..2288)
                     /locus_tag="POB55_002374"
     CDS             complement(1833..2288)
                     /locus_tag="POB55_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDI6501315.1"
                     /translation="MSTNKNDYEHMLFYFAYKTFITTADEIIEKYGMSRQHHRFLFFI
                     NKLPGITIKSLLEILEISKQGSHATLQKLKEQGLIIEKVLETDRRVKKLYSTDKGDQL
                     IAELNKAQDELLQNIYQQVGSDWYDVMEALAKGRPGFDFIKHLKDEKES"
     gene            complement(2533..3294)
                     /locus_tag="POB55_002375"
     CDS             complement(2533..3294)
                     /locus_tag="POB55_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDI6501316.1"
                     /translation="MAYISLNYHSPTIGMHQNLTVILPEDQSFFNNDITAKPLKTLML
                     LHGLSSDETTYMRYTSIERYANEHKLAVIMPNVDHSAYANMAYGHSYYDYILEVYDYV
                     HQIFPLSKKRDDNFIAGHSMGGYGTIKFALTQGDKFAKAVPLSAVFEAQNLMDLEWND
                     FSKEAIIGNLSSVKGTEHDPYYLLDKAVAEDKQIPKLLIMCGKQDFLYQDNLDFIDYL
                     SRKNVPYQFEDGPGDHDYAYWDQAIKRAITWMVND"
     gene            complement(3410..>3663)
                     /locus_tag="POB55_002376"
     CDS             complement(3410..>3663)
                     /locus_tag="POB55_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDI6501317.1"
                     /translation="DSDSDSDSDSRVTPPNNEQKAPSNPKGEVNHSNKVSKQHKTDAL
                     PETGDKSENTNATLFGAMMALLGSLLLFRKRKQDHKEKA"
BASE COUNT         1251 a          626 c          582 g         1204 t
ORIGIN      
        1 tcatatatat tcattttttg ttattctaaa tcactctgtt tacatatttt taactaaaac
       61 tacataattg ctcaaaattc ctcttttaca ctgaagacat agtcatatac tcaggaggat
      121 gtcttatgtt atcagttaaa aaagccacaa aatttgcagt tgctaacctt attatcgtgt
      181 caacggtttt tagttctgtc acacctgcaa ttgcgcaatc cgataaaagt tctaaagatg
      241 aaagtttcgc ggttggtaat actaaaaatc caaaaaatgt tatcttttta gttggcgatg
      301 gcatgggtcc atcatttaac acagcttatc gttattataa aaatgatccg catgctaaag
      361 aaatgacccc tactgctttt gattcatatt taaaaggcac aaatcgtaca tattcaaatg
      421 acccaaaaca gaatattact gattctgcag ctggcggaac agcctttagt tctggtcata
      481 agacatataa tggtgccatc ggtgttgaca gtaacaagca gaaagtaaag acggtgcttg
      541 aacgtgcaaa agaaaaaggt aagtcgactg gtcttgtttc aacagctgaa ttaacagatg
      601 caacaccagc agcatatgct gcacacgtga cttctagaga tgataaaaac gaaatcgcta
      661 aacaatttta taaagataaa atcaacggaa agcataaagt tgatgtttta ttaggcggcg
      721 gcgctaaata tttcggtaaa agtaatggca atttagataa aaaattcaaa aatgatggtt
      781 acgaccttgc cacaaacagt aaggagttat caaaatctga taaagataaa gttcttggtt
      841 tgtttgcaga taaaaatatg ccacttgcaa tagatgcttc taaagatgaa ccatcattag
      901 ctgatatgca acaaagtgca cttagtaagc ttgagcgtaa taaaaaagga ttctttttaa
      961 tggtcgaagg tgcttctata gataaatcag cgcattccaa cgatattaca ggcgttatgt
     1021 ctgaaatgga aggttttgaa aaagcattcg atgacgccat tcaatatgct aagaaacata
     1081 aagatacact tgtcgttgca acagcagatc actctactgg tggtctaaca attggtaaag
     1141 ataaaggata cgaatggaat cctcaaccga ttaaatcgat gaaacactct ggatcatata
     1201 tgacagaaaa aatggctaaa ggtgaagacc ctgaaaaagt tattaatgaa ggttatggct
     1261 ttgaattttc acaagatgat atgaaaaagg ttaaaaaaga gaataaaaag ctaaaaagac
     1321 tacttaaaaa agaaaaagac gaaaaatccg caaaagtaga aacgcaaaca aaagccctac
     1381 aacacgctat tcagaagcca gtaaatgata aatcttatac agggtggact tccgatggac
     1441 atacaggcga ggatgttaat acctacgctt atggacctag atcagaatca tttagtggta
     1501 atatcgaaaa tacagaaagc gcaaaaataa tatttgatat attcaagtaa agcataaaaa
     1561 aattcccgag cactataaca aggtgttcgg gaatttttta tggtttaatt tagtactcaa
     1621 tgttaggtgc ttggttaaca cgttcttggt attttttaaa tgcgaagtat aaaccgccaa
     1681 ctaatgttaa aataactgct aattttttca tcattcaaac tcctttctaa gatgtgttga
     1741 aactgagttc aattaaatta tatgttttta ttatacactt tttgacatat tttttaaatt
     1801 taagaatgcg aagattttta acatttctga tgctagcttt ctttttcatc tttcaaatgc
     1861 ttaataaaat caaagccagg tcgcccttta gccaatgctt ccatcacatc ataccaatcc
     1921 gaaccgactt gttgatatat attttgcaat aattcatctt gcgccttgtt caattcagca
     1981 atgagttgat cgcctttatc cgtcgaatat aattttttga cacgtcgatc agtctctaaa
     2041 actttttcaa taatgagacc ttgctctttt aatttttgaa gtgttgcatg tgatccttgt
     2101 ttagaaattt ctaatatttc tagtaatgat ttaatagtaa taccaggtaa tttattgata
     2161 aaaaacaaaa aacgatgatg ctgacggctc ataccatact tctctataat ttcatcagcg
     2221 gtagtaataa acgttttata tgcaaaataa aacaacatat gctcataatc gtttttattg
     2281 gtcgacatga tgatgactcc tttcaaagcg ttaacctcat tataataaaa tttataatgt
     2341 aagtcataca aatgatgata aaactataag ctaaatctaa gtttcacgag aaacattttt
     2401 gcgtatcagc aaattgcgcc aatcatatta atacaaattt aatttaaaca ctcagaaatg
     2461 ttagtcgtgc gtaaatagtt tgtatctctc actctgtgta tttaatgcaa ccacatattt
     2521 tccaggaaat aattaatcat tcaccatcca tgttatagca cgcttaatcg cttggtccca
     2581 atatgcataa tcatgatctc ctggtccatc ttcaaattga taaggaacat ttttacgtga
     2641 taaataatcg ataaaatcta agttgtcttg atataaaaag tcttgtttac cacacataat
     2701 gagcaatttt ggaatttgtt tatcttcagc tacagctttg tctagcaaat aatacggatc
     2761 atgttcagtt cctttaacac ttgaaagatt gccaattatg gcctcttttg aaaaatcatt
     2821 ccactctaga tccattaaat tttgcgcttc aaacacagca gataatggta cagctttggc
     2881 aaatttatcc ccttgtgtta atgcaaattt aattgtgcca tatcctccca tagagtgacc
     2941 tgctataaaa ttgtcatcac gctttttgga aagtggaaat atttgatgaa cataatcata
     3001 tacttccaaa atataatcat aatagctatg accatatgcc atgttagcat atgcgctatg
     3061 atccacattg ggcataatca cagctaattt gtgttcattc gcataccttt ctatgcttgt
     3121 atatctcata tatgtcgttt catcacttga taatccatgt aacaacatta aagtttttaa
     3181 tggtttagca gttatatcgt tattaaagaa gctttgatct tccggtaaaa tgactgtcaa
     3241 attttgatgc ataccaattg ttggtgaatg atagtttaat gaaatataag ccatacgtca
     3301 tgaccccttt ctaattctac tctatcaaca ttttacgctt aatcaattca ctttaaaatc
     3361 attttcaaca aaaaaaccga atacaaatgt attcggccta aaaaagtatt tacgcttttt
     3421 ctttatgatc ttgcttgcgt tttctaaaca atagtaatga tcctaataat gccatcattg
     3481 caccaaataa agttgcattt gtgttttcgc tcttatctcc tgtttctggt aaagcatcag
     3541 ttttgtgttg ttttgatacc ttattagaat ggtttacttc acctttagga tttgatggtg
     3601 ctttctgttc attatttggt ggtgtaactc ttgaatcgga gtcactatct gaatccgagt
     3661 cgc
//
LOCUS       DAMZKC010000195         3651 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.198, whole genome shotgun sequence.
ACCESSION   DAMZKC010000195 DAMZKC010000000
VERSION     DAMZKC010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3651
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.198"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            79..429
                     /locus_tag="POB55_002377"
     CDS             79..429
                     /locus_tag="POB55_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YppE family protein"
                     /protein_id="HDI6501318.1"
                     /translation="MNDLVESLIYEVNNMQQNFENVKSQQQDHDFYQTVKPYTEHIDS
                     MLNEIKLHREFIIEVPYMNSRKFELLIANIEQLSVECHFKRTSRKLFIEKLKSVQYDL
                     QNILDGVTKEGTDG"
     gene            422..985
                     /locus_tag="POB55_002378"
     CDS             422..985
                     /locus_tag="POB55_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HDI6501319.1"
                     /translation="MVKTVYVTGYKSFELNIFKDDAPEVHYLKQFIKHKIEQLLDEGL
                     EWVLIQGQMGIELWTAEVVIELQRTYDSLKFAVITPFQGHTEKWNEHNQSKYANIIKH
                     ADYVDSIFHTSYQGPFQFKQADQFMLEHSDQTLLIYDEEQEASPKFFKQMLVDFMDKT
                     NYTCDIVTFDELTAFINDLQWSEDQSF"
     gene            999..1343
                     /gene="gpsB"
                     /locus_tag="POB55_002379"
     CDS             999..1343
                     /gene="gpsB"
                     /locus_tag="POB55_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HDI6501320.1"
                     /translation="MSDVSLKLSAKDIYEKDFEKTMARGYRREEVDAFLDDIIADYQK
                     MADMNNEVVKLSEENHKLKKELEELRLRVATSRPQDNKSFSSNNTTTNTSSNNVDILK
                     RISNLEKAVFGK"
     gene            1406..1785
                     /gene="rnpB"
                     /locus_tag="POB55_002380"
     ncRNA           1406..1785
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="POB55_002380"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            1994..3139
                     /locus_tag="POB55_002381"
     CDS             1994..3139
                     /locus_tag="POB55_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HDI6501321.1"
                     /translation="MFQLLAVCPMGLEAVVAREIQELGYETNVENGRIFFEGDASAIV
                     KANLWLRTADRIKIVVGRFNATTFDELFEQTKALPWESIIDKEGNFPVQGRSVKSTLH
                     SVPDCQAITKKAIVERLRRAYNEKGWLNESGAKYPVEVAILKDNVLLTIDTSGSGLNR
                     RGYRLAQGEAPIKETLAASLIRLANWKGDTPLIDPFCGSGTIAIEACLIAQNIAPGFN
                     REFVSEQWNIMPANIYDDYRDEADKMADYDKEIEVYASDIDPEMVEIAKRNAEEVGLS
                     DIIKFSVKDVNTLTIDTEEPVALIGNPPYGERIGDREEVEEMYRYIGKLMKQHPFLST
                     YILTSNKEFEYLVDRKATKRRKLFNGYIECTYYQYWGKKTERKTIEN"
     gene            3223..3555
                     /locus_tag="POB55_002382"
     CDS             3223..3555
                     /locus_tag="POB55_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4889 domain-containing protein"
                     /protein_id="HDI6501322.1"
                     /translation="MGKKMGLGLSIALIVIGIAVVCLMIFSSQKTTYFGYMNSNTNAE
                     KVVSEKDGLVKHNIKVEPSNDFKPKKGDFVKLVSKDDGKTFYKQEIVKHDDVPHGLMM
                     KIHDMHMN"
BASE COUNT         1364 a          480 c          704 g         1103 t
ORIGIN      
        1 taaatcaaat tatatagagt gttaaaacat gacgaataca gtatattgtg ataaattaaa
       61 aatgtaggag gcgtcctcat gaatgattta gtcgaatcac taatttatga agttaacaac
      121 atgcaacaaa attttgaaaa tgtgaaatca caacaacaag atcatgattt ttaccaaact
      181 gtaaagccat atactgaaca tattgatagc atgctcaatg agatcaaatt acatcgtgaa
      241 tttattatag aagtacctta tatgaattca aggaaatttg agctactgat tgctaacatt
      301 gaacaacttt ctgtcgaatg tcattttaag cgaacaagtc gaaagttatt tatagaaaag
      361 cttaaaagtg ttcaatatga tttacaaaat atattagatg gcgtaacaaa agagggtact
      421 gatggttaaa acagtttatg taacaggtta caaatcattc gaattaaaca tttttaaaga
      481 tgacgcacct gaagtacatt atttaaaaca atttataaaa cataaaattg aacaactgtt
      541 ggatgaagga ttagaatggg tgttaataca agggcaaatg ggtatagaat tatggactgc
      601 agaagttgtt attgaattac aacgaacata tgattcgtta aagtttgctg taattacacc
      661 gttccaagga catacagaaa agtggaatga acataatcaa agtaagtatg ccaacataat
      721 taagcatgca gattatgttg atagtatttt tcatacgtcg tatcagggtc cttttcaatt
      781 taaacaagca gatcaattta tgcttgaaca ttcggatcaa acattgctca tttatgatga
      841 ggaacaagag gcaagtccta agttcttcaa gcagatgtta gttgatttta tggataaaac
      901 aaactatact tgtgatattg tgacgttcga tgaattaaca gcattcatca atgacttaca
      961 gtggtctgaa gatcaaagtt tctaatgagg tggaaaaaat gtcagatgtt tcattgaaat
     1021 tatcagcaaa agatatttat gaaaaagatt ttgaaaaaac gatggctcgt ggctatagaa
     1081 gagaagaagt agatgcattt ttagatgaca ttattgctga ttatcaaaaa atggccgata
     1141 tgaataatga agttgtaaaa ttatcagaag agaatcataa acttaaaaaa gaattagaag
     1201 aattaagact acgtgtagca acatcaagac ctcaggacaa taaaagtttt tcttcgaata
     1261 atacaacaac aaatacatct tcaaataatg tagatatttt aaaacgtatt tcaaacttag
     1321 aaaaagctgt atttggtaaa taattactaa atacaaaagt ttaactgtct tactaataat
     1381 gactatgtta taattttaaa agtgatattt tgggtaatcg ctatattata tagaggaaag
     1441 tccatgctca cacagtctga gatgattgta gtgttcgtgc ttgatgaaac aataaatcaa
     1501 ggcattaatt tgacggcaat gaaatatcct aagtctttcg atatggatag agtaatttga
     1561 aagtgccaca gtgacgtagc ttttatagaa atataaaagg tggaacgcgg taaacccctc
     1621 gagtgagcaa tccaaatttg gtaggagcac ttgtttaacg gaattcaacg tataaacgag
     1681 acacacttcg cgaaatgaag tggtgtagac agatggttat cacctgagta ccagtgtgac
     1741 tagtgcacgt gatgagtacg atggaacaga acatggctta tagaaatatc actactagtt
     1801 tagctctcct agatgatgga gagctttttt catgaaaaga acacttaaaa ttaacgcctt
     1861 gtcttgatat aatgacactg ccttgtttta aaatagtaag cggatgcgtt aatgtatcag
     1921 cgattaaatt tgttggaaat gtataaaaaa cacaagctaa gaataaaata cctgtataaa
     1981 aggagaatca tatatgtttc aattacttgc agtttgtccg atgggattag aagctgttgt
     2041 tgctagggaa attcaagaat taggctatga aacaaatgtt gaaaatggtc gtatattttt
     2101 tgaaggagac gcaagtgcaa ttgtaaaggc aaatttatgg ttgcgcacag cagaccgaat
     2161 caaaattgtt gttggacgtt ttaacgcaac aacgtttgac gaattattcg aacaaaccaa
     2221 agcgctccct tgggaatcta taattgataa agaaggtaac ttcccagttc aaggtagaag
     2281 cgttaaatca acactacata gtgtgccaga ttgtcaggcg ataactaaga aagctattgt
     2341 tgaacgatta cgtcgcgcat ataacgaaaa aggttggtta aatgaatcag gtgccaaata
     2401 ccctgttgaa gttgccattt taaaagataa tgtattattg actatagaca catcaggttc
     2461 tggtttgaac agacgtggat atagattagc acaaggtgaa gcaccaatta aagaaacgtt
     2521 ggcagcaagt ttaatccgtc ttgcaaactg gaaaggtgat acacctttaa ttgatccatt
     2581 ttgcggttcg ggtacaatag ctatagaagc ttgtttaatt gctcaaaata tcgcacctgg
     2641 ttttaatcgc gagttcgtat cagagcaatg gaacatcatg ccagcaaata tttatgatga
     2701 ttaccgtgat gaagcggata agatggctga ttatgataaa gaaatcgaag tatatgcttc
     2761 tgatattgat ccagaaatgg tagagattgc taagcgtaac gctgaagaag ttgggttgtc
     2821 tgatattatt aaatttagtg taaaagatgt caatacatta acaattgata cagaagaacc
     2881 ggtggcgtta attggaaatc ctccatatgg tgaacgtatt ggtgatcgtg aagaagttga
     2941 agaaatgtac cgttatattg gtaaactaat gaaacaacat ccatttttat ctacatacat
     3001 tttaacaagt aataaagaat ttgaatattt agtagatcgt aaagcaacga agcgacgtaa
     3061 gttatttaat ggatatatag aatgtactta ttatcaatac tggggtaaaa agacagaacg
     3121 taaaactatc gaaaactaaa gtgtttctaa tgcgtgacta aaattagtat taattatgtt
     3181 ctcatgataa taggtgtttt tgaaaaatgg aggagtctat aaatgggtaa aaaaatgggt
     3241 ctaggtttat ctatagcatt gattgttatt ggtattgccg ttgtatgttt aatgattttt
     3301 tctagtcaaa aaacgactta ttttggttat atgaatagta atacaaatgc agaaaaagtt
     3361 gtaagtgaaa aagatggatt agtcaaacat aatatcaaag tagaaccatc taatgatttc
     3421 aagccgaaaa aaggagactt tgtaaaatta gtttctaaag atgatgggaa gacattttat
     3481 aaacaagaga ttgttaaaca tgatgacgtc ccacacggtt taatgatgaa aattcacgac
     3541 atgcatatga attaataaaa aagcatctat aacgtaattt tgaagaagta gagttatctt
     3601 cttatgcgtt ttagatgctt ttttatcttc aaattcaaaa aatcggacag a
//
LOCUS       DAMZKC010000196         3645 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.199, whole genome shotgun sequence.
ACCESSION   DAMZKC010000196 DAMZKC010000000
VERSION     DAMZKC010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3645
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.199"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            172..627
                     /locus_tag="POB55_002383"
     CDS             172..627
                     /locus_tag="POB55_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDI6501323.1"
                     /translation="MVKHLQDHIQFLEQFINNVNALTAKMLKDLQNEYEISLEQSNVL
                     GMLNKEPLTISEITQRQGVNKAAVSRRIKKLIDAKLVKLDKPNLNIDQRLKFITLTDK
                     GRAYLKERNAIMTDIAQDITNDLNSEDIENVRQVLEVINHRIKTYSNHK"
     gene            867..2249
                     /locus_tag="POB55_002384"
     CDS             867..2249
                     /locus_tag="POB55_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501324.1"
                     /translation="MKSCPKCGQQAQDDVQICTQCGHKFDSRQALYRKSTDEDIQTNN
                     IKMRKMVPWAIGFFILILIIILFFLLRNFNSPEAQTKILVNAIENNDKQKVATLLSTK
                     DNKVDSEEAKVYINYIKDEVGLKQFVSDLKNTVHKLNKSKTSVASYIQTRSGQNILRV
                     SKNGTRYIFFDNMSFTAPTKQPIVKPKEKTKYEFKSGGKKKTVIAEANKVTPIGNFIP
                     GTYRIPAMKSTENGDFAGYLKFDFRQSNSETVDVTEDFEEANITVTLKGDTKLNDSSK
                     KVTINDREMAFSSSKTYGPYPQNKDITISASGKAKDKTFTTQTKTIKASDLKYNTEIT
                     LNFDSEDIEDYVEKKEKEENSLKNKLIEFFAGYSLANNAAFNQSDFDFVSSYIKKGSS
                     FYDDVKKRVSKGSLMMISSPQIIDAEKHGDKITATVRLINENGKQVDKEYELEQGSQD
                     RLQLIKTSEK"
     gene            2280..2408
                     /locus_tag="POB55_002385"
     CDS             2280..2408
                     /locus_tag="POB55_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501325.1"
                     /translation="MEYKQHPFKVFTISVNYFEGIVFCGKIYEVLRFISFYTFYKL"
     gene            2518..>3645
                     /locus_tag="POB55_002386"
     CDS             2518..>3645
                     /locus_tag="POB55_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HDI6501326.1"
                     /translation="MNDLTHFNKRSPIFIIILGSLTAIGALSIDMFLPGLPDIRHDFQ
                     TTTSNAQLTLSMFMIGLAFGNLFAGPISDSTGRRKPLIIAMIIFTLASLGIVFVHNIW
                     LMVALRFLQGVTGGAAAVISRAIASDMYSGNELTKFMALLMLVNGIAPVVAPTIGGII
                     LNYSVWRMVFVILTIFGFVMVIGSLLKVPESLTVTNRESSSGLKTMFKNFKILLKTPR
                     FVLPMLIQGMTFVILFTYISASPFIIQKIYGMTAIQFSWMFAGIGITLIISSQLTGYL
                     VDFIDSQKLMRGMTMIQIIGVILVTIVLLNHWNFWILAIGFIILIAPVTGVATLGFTI
                     AMDESSSGRGSSSSLLGLVQFLFGGVASPLVGVKGEDNPIPY"
BASE COUNT         1338 a          453 c          672 g         1182 t
ORIGIN      
        1 tattaaatat aaatgtagat gatttaattt attaaagcaa ggagaacgca gcaacatgta
       61 agtcttaatt tgttatatta tatatgggtc aatatttttg tgttttttag atgtgcgata
      121 caatgattgc tgaggatact ttataaaaat agtaattagg gagaaataat tatggtaaaa
      181 catttacaag accatattca atttttagag cagtttataa ataacgttaa cgcattaact
      241 gcaaaaatgt tgaaagattt acaaaatgaa tatgaaattt cattagagca gtctaacgta
      301 ttaggtatgt taaataaaga acctttgaca attagtgaaa tcacgcaaag acaaggtgta
      361 aataaggccg cagtaagccg acgaattaaa aagttaatcg atgctaaatt agttaagtta
      421 gataaaccaa atttaaatat tgatcaacgt ttgaaattca taaccttaac tgacaaaggt
      481 agagcatatt tgaaagaacg taatgcgatt atgacagata ttgcgcaaga tattactaat
      541 gatttaaatt ctgaagatat tgaaaatgta aggcaagtat tagaagtcat caatcatcgc
      601 attaaaacat atagcaatca taaataggtt tttgaaggtg ttcaaatata agactaaata
      661 tgtatgtatt gaacaccttc tttttagtta taattgagag agtactttca taaataatgt
      721 aataaggtac aattctaaag taaagttaat catttcttga attgtattct tgggataata
      781 tttgtaaaat aataatggta aaataatgaa ataatttttg tgatatacat ataacaattg
      841 aagaggaaca gaattgaggt gaaagtatga aatcttgccc gaagtgcgga caacaagcac
      901 aagatgatgt acagatatgt acacaatgtg gacataaatt tgatagtcgt caagcattat
      961 atagaaagtc aaccgatgaa gatatacaaa ctaataacat caagatgaga aaaatggtgc
     1021 cttgggcaat tggttttttc attttaatct taattattat attgttcttt ttactaagaa
     1081 acttcaattc acctgaagcg caaactaaaa tattagtgaa tgcgattgag aataatgata
     1141 aacaaaaagt agcaacatta ttaagtacta aagataataa agtagattct gaagaagcga
     1201 aagtatacat taactatatc aaagatgaag ttgggcttaa gcaatttgtc agcgacctta
     1261 aaaatacggt acataaattg aataagagta agaccagcgt agcttcttat attcaaacca
     1321 gatctggtca aaatatatta cgtgtaagta aaaatggcac gcgttatatc tttttcgata
     1381 atatgagctt tactgcacct accaagcaac caattgttaa accgaaagaa aaaacaaaat
     1441 atgagtttaa atctggtggt aagaaaaaga cggttatagc tgaagcaaat aaagtgacgc
     1501 caataggtaa ttttataccg gggacatata gaattccagc tatgaaatca actgagaacg
     1561 gtgattttgc aggctattta aaatttgatt ttagacaaag taattctgaa acggtagatg
     1621 ttactgaaga ttttgaagaa gcaaatataa ctgtaacttt aaaaggcgat acaaaattaa
     1681 atgatagttc taaaaaagta actataaatg accgtgaaat ggcattttca agttccaaaa
     1741 cgtatggtcc atatccacaa aataaagata ttaccatttc agcttcaggt aaagcgaaag
     1801 ataaaacatt tacaacacag acgaagacga ttaaagctag cgatttaaaa tacaatacag
     1861 aaataacttt gaattttgac agtgaagata tcgaagacta tgttgaaaag aaagaaaaag
     1921 aagaaaacag cttgaagaac aaattgatag aattctttgc tggatattct ttagcgaata
     1981 atgctgcgtt taatcagtcg gattttgatt ttgtatcatc atatataaaa aaaggatcat
     2041 ctttttatga tgatgtaaag aagcgtgtat ctaaaggaag tttaatgatg attagttcac
     2101 cacaaattat agatgctgaa aaacatggtg ataagattac cgcaaccgta agattaataa
     2161 atgaaaacgg taagcaagta gataaagaat atgagcttga acaaggctcg caagaccgct
     2221 tgcaattaat caagacatca gaaaaataga gtataaaact taaataagat gcatgtgtca
     2281 tggaatacaa acaacatcct ttcaaagtat ttacaattag tgtgaattac tttgaaggga
     2341 ttgttttttg tggaaaaatt tatgaagtgc tgaggtttat atcattttat accttttata
     2401 agttgtaatg atgttcatga aacttgtgac taatgctaat atataaaaga gcatatttaa
     2461 atttagtttc tgttcgtgat agttataaac taatcattat ataaaggaga tagtcatatg
     2521 aatgatttaa cgcattttaa caagcgttca ccgatattta ttattatttt aggatctttg
     2581 actgccattg gagcattatc gatagatatg tttttgcctg gattaccaga tattagacat
     2641 gattttcaaa caacgacgtc taacgcacaa ttaacattat cgatgtttat gattggttta
     2701 gcgtttggta atttgtttgc tggtccaatt tcagattcaa ctgggcgcag aaagccgctt
     2761 attatagcta tgattatttt tacattagca agtttaggta ttgtttttgt acataatatt
     2821 tggctgatgg ttgcattaag atttcttcaa ggagtgacag gtggcgcagc agcagttatt
     2881 tcaagagcta tagcaagtga tatgtatagt ggtaatgaac taactaagtt tatggcatta
     2941 ttgatgcttg ttaatggtat tgcaccagtt gttgcgccaa caataggggg cataatttta
     3001 aattattctg tctggcgtat ggtctttgtc attctgacta tctttggctt cgtcatggtc
     3061 ataggttctt tattaaaagt acctgaatca ttaacagtga caaatcgtga gtcaagtagt
     3121 ggattaaaga caatgtttaa aaacttcaaa atattattga agacgccacg ttttgtatta
     3181 ccaatgctca ttcaaggtat gacatttgta atattattta cttatatttc tgcgtctccg
     3241 tttattattc aaaaaattta tggcatgact gcaatacaat ttagttggat gtttgcagga
     3301 attggtatta cgctcattat atcaagtcaa cttactgggt accttgttga ttttatagac
     3361 tcacaaaaat tgatgagagg catgacgatg attcagatta tcggtgtaat tttagtgacg
     3421 attgtattgc ttaatcattg gaacttttgg attttagcga taggatttat cattttaata
     3481 gcaccagtga caggtgttgc gacacttggt tttacaatag ctatggatga aagtagtagt
     3541 ggtagaggga gttcatcaag tttattaggt ttagtgcagt ttttatttgg tggtgttgca
     3601 tcgccattag tgggggtaaa aggtgaagat aatcctatac cttac
//
LOCUS       DAMZKC010000197         3518 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.200, whole genome shotgun sequence.
ACCESSION   DAMZKC010000197 DAMZKC010000000
VERSION     DAMZKC010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3518
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.200"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            183..485
                     /gene="gatC"
                     /locus_tag="POB55_002387"
     CDS             183..485
                     /gene="gatC"
                     /locus_tag="POB55_002387"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HDI6501327.1"
                     /translation="MTKVTREEVEHIANLARLQISPEETEEMANTLESILDFAKQNDS
                     ADTEGVEPTYHVLDLQNVLREDKAIKGIPQELALKNAKETEDGQFKVPTIMNEEDA"
     gene            487..1944
                     /gene="gatA"
                     /locus_tag="POB55_002388"
     CDS             487..1944
                     /gene="gatA"
                     /locus_tag="POB55_002388"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HDI6501328.1"
                     /translation="MSIRYESVENLLTLIKDKKIKPSDVVKDIYDAIEETDPTIKSFL
                     ALDKENAIKKAQELDELQAKDQMDGKLFGIPMGIKDNIITNGLETTCASKMLEGFVPI
                     YESTVMEKLHKENAVLIGKLNMDEFAMGGSTETSYFKKTVNPFDHKAVPGGSSGGSAA
                     AVAAGLVPFSLGSDTGGSIRQPAAYCGVVGMKPTYGRVSRFGLVAFASSLDQIGPLTR
                     NVKDNAIVLEAISGADVNDSTSAPVDDVDFTSEIGKDIKGLKVALPKEYLGEGVADDV
                     KEAVQNAVETLKSLGAVVEEVSLPNTKFGIPSYYVIASSEASSNLSRFDGIRYGYHSK
                     EAHSLEELYKMSRSEGFGKEVKRRIFLGTFALSSGYYDAYYKKSQKVRTLIKNDFDKV
                     FENYDVVVGPTAPTTAFNLGEEIDDPLTMYANDLLTTPVNLAGLPGISVPCGQSNGRP
                     IGLQFIGKPFDEKTLYRVAYQYETQYNLHDVYEKL"
     gene            1957..3384
                     /gene="gatB"
                     /locus_tag="POB55_002389"
     CDS             1957..3384
                     /gene="gatB"
                     /locus_tag="POB55_002389"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HDI6501329.1"
                     /translation="MHFETVIGLEVHVELKTDSKMFSPSPAHFGAEPNSNTNVIDLAY
                     PGVLPVVNKRAVDWAMRAAMALNMEIATESKFDRKNYFYPDNPKAYQISQFDQPIGEN
                     GYIDIEVDGETKRIGITRLHMEEDAGKSTHKGEYSLVDLNRQGTPLIEIVSEPDIRSP
                     KEAYAYLEKLRSIIQYTGVSDVKMEEGSLRCDANISLRPYGQEKFGTKAELKNLNSFN
                     YVRKGLEYEEKRQEEELLNGGEIGQETRRFDESTGKTILMRVKEGSDDYRYFPEPDIV
                     PLYIDDAWKERVRQTIPELPDERKAKYVNELGLPAYDAHVLTLTKEMSDFFESTIEHG
                     ADVKLTSNWLMGGVNEYLNKNQVELLDTKLTPENLAGMIKLIEDGTMSSKIAKKVFPE
                     LAAKGGNAKQIMEDNGLVQISDEATLLKFVNEALDNNEQSVEDYKNGKGKAMGFLVGQ
                     IMKASKGQANPQLVNQLLKQELDKR"
BASE COUNT         1299 a          517 c          667 g         1035 t
ORIGIN      
        1 aatattgaat tgggttgtct aatattaaaa gagtgttgtt ttcataaaaa atgatttgat
       61 aaacgaatga taatgtttat acagtttata tgaaattaaa gtagcacctc ataaatactt
      121 agatttttaa ttggaaattt gatacaattt agtgatgaat gacttaaagg aggcttttat
      181 taatgacaaa agtaacacgt gaagaagttg agcatatcgc gaatcttgca agacttcaaa
      241 tttctcctga agaaacggaa gaaatggcca acacattaga aagcatttta gattttgcaa
      301 aacaaaatga tagcgctgat acagaaggcg ttgaacctac atatcacgtt ttagatttac
      361 aaaacgtttt acgtgaagat aaagcaatta aaggtattcc gcaagaatta gctttgaaaa
      421 atgccaaaga aacagaagat ggacaattta aagtgcctac aatcatgaat gaggaggacg
      481 cgtaagatga gcattcgcta cgaatcggtt gagaatttat taactttaat aaaagacaaa
      541 aaaatcaaac catctgatgt tgttaaagat atatatgatg caattgaaga gactgatcca
      601 acaattaagt cttttctagc gctggataaa gaaaatgcaa tcaaaaaagc gcaagaattg
      661 gatgaattac aagcaaaaga tcaaatggat ggcaaattat ttggtattcc aatgggtata
      721 aaagataaca ttattacaaa cggattagaa acaacatgtg caagtaaaat gttagaaggt
      781 tttgtgccaa tttacgaatc tactgtaatg gaaaaactac ataaagagaa tgccgtttta
      841 atcggtaaat taaatatgga tgagtttgca atgggtggtt caacagaaac atcttatttc
      901 aaaaaaacag ttaacccatt tgaccataaa gcagtaccag gtggttcatc aggtggatct
      961 gcagcagcag ttgcagctgg tttagtacca tttagcttag gttcagacac aggtggttca
     1021 attagacaac cggctgcata ttgtggcgtt gtcggtatga aaccaacata cggtcgtgta
     1081 tctcgatttg gattagttgc ttttgcatct tcattagacc aaattggtcc attgactcga
     1141 aatgtaaaag ataatgcaat cgtattagaa gctatttctg gtgcagatgt taatgactct
     1201 acaagtgcac cagttgatga tgtagacttt acatctgaaa ttggtaaaga tattaaagga
     1261 ttaaaagttg cattacctaa agaatactta ggtgaaggtg tagctgatga cgtaaaagaa
     1321 gcagttcaaa acgctgtaga aactttaaaa tctttaggtg ctgtcgttga ggaagtatca
     1381 ttgccaaata ctaaatttgg tattccatca tattacgtga ttgcatcatc agaagcttcg
     1441 tcaaaccttt ctcgttttga cggaattcgt tatggttatc attctaaaga agctcattca
     1501 ttagaagaat tatataaaat gtcaagatct gaaggtttcg gtaaagaagt aaaacgtcgt
     1561 attttcttag gtacatttgc attaagttca ggttactacg atgcttacta taaaaaatct
     1621 caaaaagtta gaacattgat taaaaatgac tttgataaag tattcgaaaa ttatgatgta
     1681 gtagttggtc caacagcgcc tacaactgcg tttaatttag gtgaagaaat tgatgatcca
     1741 ttaacaatgt atgccaatga tttattaaca acaccagtaa acttagctgg attacctggt
     1801 atttctgttc cttgtggaca atcaaatggc cgaccaatcg gtttacagtt cattggtaaa
     1861 ccattcgatg aaaaaacgtt atatcgtgtc gcttatcaat atgaaacaca atacaattta
     1921 catgacgttt atgaaaaatt ataaggagtg gaaatcatgc attttgaaac agttatagga
     1981 cttgaagttc acgtagagtt aaaaacggac tcaaaaatgt tttctccatc accagcgcat
     2041 tttggagcag aacctaactc aaatacaaat gttatcgact tagcatatcc aggtgtctta
     2101 ccagttgtta ataagcgtgc agtagactgg gcaatgcgtg ctgcaatggc actaaatatg
     2161 gaaatcgcaa cagaatctaa gtttgaccgt aagaactatt tctatccaga taatccaaaa
     2221 gcatatcaaa tttctcaatt tgatcaacca attggtgaaa atggatatat cgatatcgaa
     2281 gtcgacggtg aaacaaaacg aatcggtatt actcgtcttc acatggaaga agatgctggt
     2341 aagtcaacac ataaaggtga gtattcatta gttgacttga accgtcaagg tacaccgcta
     2401 attgaaatcg tatctgaacc agatattcgt tcacctaaag aagcatatgc atatttagaa
     2461 aaattgcgtt caattattca atacactggt gtatcagacg ttaagatgga agagggatct
     2521 ttacgttgtg atgctaacat ctctttacgt ccatatggtc aagaaaaatt tggtactaaa
     2581 gccgaattga aaaacttaaa ctcatttaac tatgtacgta aaggtttaga atatgaagaa
     2641 aaacgccaag aagaagaatt gttaaatggt ggagaaatcg gacaagaaac acgtcgattt
     2701 gatgaatcta caggtaaaac aattttaatg cgtgttaaag aaggttctga tgattaccgt
     2761 tacttcccag agcctgacat tgtaccttta tatattgatg atgcttggaa agagcgtgtt
     2821 cgtcagacaa ttcctgaatt accagatgaa cgtaaagcta agtatgtaaa tgaattaggt
     2881 ttacctgcat acgatgcaca cgtattaaca ttgactaaag aaatgtcaga tttctttgaa
     2941 tcaacaattg aacacggtgc agatgttaaa ttaacatcta actggttaat gggtggcgta
     3001 aacgaatatt taaataaaaa tcaagtagaa ttattagata ctaaattaac accagaaaat
     3061 ttagcaggta tgattaaact tatcgaagac ggaacaatga gcagtaaaat tgcgaagaaa
     3121 gtcttcccag agttagcagc taaaggtggt aatgctaaac agattatgga agataatggc
     3181 ttagttcaaa tttctgatga agcaacactt ctaaaatttg taaatgaagc attagacaat
     3241 aacgaacaat cagttgaaga ttacaaaaat ggtaaaggca aagctatggg cttcttagtt
     3301 ggtcaaatta tgaaagcgtc taaaggtcaa gctaatccac aattagtaaa tcaactatta
     3361 aaacaagaat tagataaaag ataatttaaa tcatcaaact atgaagattt aaaaaataaa
     3421 cccttgattg ctgacttaga tgcaatcgag ggtttattta tatctataga agtcatatta
     3481 cttttaactt tattcattgt acatgttaat ggtaaaaa
//
LOCUS       DAMZKC010000198         3513 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.201, whole genome shotgun sequence.
ACCESSION   DAMZKC010000198 DAMZKC010000000
VERSION     DAMZKC010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3513
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.201"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            4..1494
                     /locus_tag="POB55_002390"
     CDS             4..1494
                     /locus_tag="POB55_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDI6501330.1"
                     /translation="MNYFVGNSLGVNLTGIEKAIINRLNLFKEMGRPAQCVFLSWNRY
                     LYRNAQNYITSSDYINMYDFFQEATYLERNEPFDWLSYWTDECHYTLKHVENSHDFRI
                     YDQERFLMYAHFQDPKYRILDYVNHFDSQRRKVKRDFYDVRGFLSCSRILVDKQQTLC
                     EFFYNPEGDTKLEKYFSYKDGKPEVQKIIVYYANKQYFFNNETELGAFFIKQLYQHGD
                     LFFSDRNVYTAPIFNLTPESIPVVAVLHSTHIKNIDALDSSPFKNVYKAMFENLSRYR
                     AIIVSTEQQKLDIEKRINHTIPVVNIPVGYSETIDTPVQTLDQRSVKLISVARYSPEK
                     QLHQQIELIKRLVSYVPKIELHMYGFGSESKKLNELIQKYGLENHVYLRGFLSNLDQE
                     YSDAYLSLITSNMEGFSLALLESLAHGVPVISYDIKYGPNELITPDFNGYLITKNDED
                     ALFDKVKYVIDHPEVQQRLSKGSLAKAQQYSKASLIKQWDQFVRLI"
     gene            complement(1618..3090)
                     /locus_tag="POB55_002391"
     CDS             complement(1618..3090)
                     /locus_tag="POB55_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDI6501331.1"
                     /translation="MNYILGTILESKITGVEKAQINRLKLFKQHGISSKCVYVKWNPY
                     SYTYAKQHQIENDVFTMYDYFQKAINYKKTKQVNWIQYWEKSCRYTLKFVENSNDVRI
                     YDEEQFIMYAHFLDKQYHHLNYVNYFDHKRRKVKRELYDGRGFLSCSRILGEGQRIVL
                     ENYYTPNGEIVIQKYFDDIKGKNTLTKVILNEDQHQQFFDTEDELVQYFLHQLCKNND
                     QIILDRPHELGNVIAGLNQSIPVIVVLHSTHLADTGNGIKSFYKTVFNNLTRYKAIVV
                     STEQQCQDISQYIENKIPVINIPVGYVANLKYQFDINQKEKNHIISIARLVENKQIKH
                     QVEVIKQLVTKHPNIQLNIYGHGNGLSEYRQLVEDYHLSEHVKFHGFKTHINEEIAKA
                     ELMLSTSKMEGFGLAILESLSVGTPVISYDVDYGPSELIQDGFNGYLVPQGDINQMVE
                     KVDQLLNNTQKLQQFSINSIESAQQYNATTISTKWQNILN"
     gene            complement(3209..>3513)
                     /locus_tag="POB55_002392"
     CDS             complement(3209..>3513)
                     /locus_tag="POB55_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDI6501332.1"
                     /translation="SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDAGKH
                     TPVKPMSTTKDHHNKAKALPETGSENNGSNNATLFGGLFAALGSLLLFGRRKKQNK"
BASE COUNT         1123 a          552 c          543 g         1295 t
ORIGIN      
        1 taaatgaatt attttgtagg taatagttta ggtgttaatt taactgggat agagaaagca
       61 attattaatc gtttaaattt atttaaagaa atgggaagac cagcgcaatg tgtatttctg
      121 tcatggaata gatatttata tagaaatgcc caaaattata ttactagttc tgactatatt
      181 aatatgtatg atttcttcca agaagcaaca tatttagaac gcaatgaacc atttgactgg
      241 ttgtcgtatt ggactgatga atgtcattat actttaaaac atgttgaaaa tagtcatgac
      301 tttagaatat atgatcaaga gcgtttctta atgtatgccc attttcaaga tcctaaatat
      361 cgtatattag attatgtgaa tcattttgat agtcaaagac gaaaagtaaa acgtgatttt
      421 tatgatgtgc gtggcttttt aagttgtagt aggattcttg ttgataaaca acaaacatta
      481 tgtgaatttt tctataatcc agaaggtgac acaaaattag aaaagtattt ttcttacaag
      541 gatggcaaac cagaagtaca aaaaattatt gtttactatg ctaacaaaca atatttcttt
      601 aataatgaaa cggaattagg cgcatttttc attaaacaac tatatcaaca tggggattta
      661 tttttcagtg atcgtaatgt gtacacagca cctattttca acttaacgcc tgagtcaatc
      721 ccagtagtag ctgtgcttca cagtacacac attaaaaata ttgatgcatt agattcatcg
      781 ccatttaaaa atgtatataa agcgatgttt gaaaatttat ctcgatatcg tgcaattatc
      841 gtttctactg agcagcaaaa attagatatt gaaaaacgta ttaaccatac tattccagtt
      901 gttaatattc ctgtcggcta tagtgaaaca attgatacac cagtccaaac attagatcag
      961 cgcagtgtta aattaatttc cgttgctcgt tattcacctg aaaaacaatt acatcaacaa
     1021 attgaattaa ttaaacgctt agtatcgtac gtcccaaaaa tagagcttca tatgtatggt
     1081 tttggttctg aaagtaaaaa attaaatgaa ttgatacaaa aatatggatt ggaaaatcat
     1141 gtttacttac gcggcttttt aagcaatcta gaccaagaat atagcgatgc ctatttgagt
     1201 ttaataacaa gtaacatgga aggtttctct ttagcgctac ttgaatcttt agcacatggt
     1261 gttccagtta ttagttatga tattaaatat ggacctaacg aattgattac gcctgatttc
     1321 aatggttatt taattactaa aaatgatgaa gatgccctat ttgataaagt aaaatatgtg
     1381 attgatcatc ctgaagtaca acaacgactg tcaaaaggta gtttagcaaa agcgcaacaa
     1441 tatagtaaag cgtcgttaat taaacaatgg gaccaatttg ttcgtcttat ataaatgtac
     1501 tttaccttta tttttacaaa aatagcattt tcctatgtca tttaactaaa catgtaagtt
     1561 cgtatgaacg aggtttgtta aatagatgat tctaggaaaa tgcttttttc ttttgactta
     1621 gtttaaaata ttttgccact ttgtactgat agtagttgca ttgtactgtt gtgcagattc
     1681 tatgctatta atcgaaaact gttgcaattt ttgagtatta tttagtaatt ggtcgacctt
     1741 ttcaaccatt tgattgatgt caccttgagg tactaaatag ccattaaatc catcttgaat
     1801 cagttctgat ggcccataat ctacatcata actgatcact ggtgtaccta ctgaaagcga
     1861 ctctaaaatt gctaagccaa aaccttccat tttacttgtc gataacatca gttctgcttt
     1921 agcaatctct tcattaatat gcgtcttaaa accatgaaat tttacatgtt ccgataaatg
     1981 ataatcttct acaagttgtc gatattctga caaaccattt ccatgtccat aaatattcaa
     2041 ttgaatattg ggatgttttg ttactaattg cttgatcact tcaacttgat gtttaatttg
     2101 tttattttca acgaggcgag caattgatat gatatgattt ttctcctttt gattgatgtc
     2161 aaattgatac tttaaatttg ccacgtagcc aaccggaata ttgataactg gtattttatt
     2221 ttcaatatat tgtgaaatat cttggcattg ctgttctgtt gatacaacaa tcgctttata
     2281 acgtgttaaa ttattaaaca ctgttttata aaaacttttt ataccattac cggtgtcggc
     2341 taaatgtgta ctgtggagca caacaataac tggaatactt tgatttaatc ccgctataac
     2401 atttcctaat tcatgaggac gatctaatat gatttgatca ttatttttac ataattgatg
     2461 gagaaaatat tgaactaatt catcttctgt atcaaaaaat tgttgatgct gatcttcatt
     2521 taagataacc tttgtgagcg tgtttttccc ttttatatcg tcgaaatatt tttggatgac
     2581 gatttcccca ttaggtgtat agtaattttc gagtacaatc cgttgtcctt cacctaaaat
     2641 tcgagaacaa cttaaaaagc ctcttccatc atacaattcg cgttttactt ttcttctttt
     2701 atgatcaaaa taattcacat agtttaaatg atgatactgt ttatctaaaa aatgagcata
     2761 cattataaat tgctcttcat catatattct gacatcattt gaattttcca caaatttcaa
     2821 tgtgtaccta catgactttt cccaatactg tatccagtta acttgctttg tctttttata
     2881 attgattgct ttttgaaaat agtcatacat tgtaaataca tcattttcaa tctgatgttg
     2941 cttcgcatat gtgtatgaat aaggattcca tttaacatat acacattttg aagatatgcc
     3001 gtgttgtttg aacaacttca atctatttat ttgcgctttt tctacacctg taattttact
     3061 ttctaaaatt gttcctaaaa tgtaattcat attatcgcct catataagtt ttattccgta
     3121 tctttattgt ttattttata tgaaaaatac atctattgca tgtgtaatta taaaaaaaac
     3181 aggccacaag gacctgggtc atattgtatt atttgttttg ttttttgcga cgaccgaata
     3241 acaataatga acctaatgct gcaaataatc caccaaataa cgttgcgtta tttgagccgt
     3301 tattttcact acctgtttct ggtaatgctt ttgctttatt gtgatggtct ttagtagtac
     3361 tcattggttt aacaggtgta tgttttcctg catccgagtc cgaatcgcta tctgagtctg
     3421 aatcactgtc tgagtctgag tcgctatctg agtctgagtc gctgtccgaa tctgagtcgc
     3481 tatctgagtc tgagtcgcta tctgaatctg aat
//
LOCUS       DAMZKC010000199         3508 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.202, whole genome shotgun sequence.
ACCESSION   DAMZKC010000199 DAMZKC010000000
VERSION     DAMZKC010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3508
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.202"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            121..915
                     /gene="tagH"
                     /locus_tag="POB55_002393"
     CDS             121..915
                     /gene="tagH"
                     /locus_tag="POB55_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acids export ABC transporter
                     ATP-binding subunit TagH"
                     /protein_id="HDI6501333.1"
                     /translation="MNVSVNIKNVTKEYRIYRTNKERMKDALIPKHKNKTFFALDDIS
                     LKAYEGDVIGLVGINGSGKSTLSNIIGGSLSPTVGKVDRNGEVSVIAISAGLSGQLTG
                     IENIEFKMLCMGFKRKEIKAMTPKIIEFSELGEFIYQPVKKYSSGMRAKLGFSINITV
                     NPDILVIDEALSVGDQTFAQKCLDKIYEFKEQNKTIFFVSHNLGQVRQFCTKIAWIEG
                     GKLKDYGELDDVLPKYEAFLNDFKKKSKAEQKEFRNKLDESRFVIK"
     gene            complement(976..1740)
                     /locus_tag="POB55_002394"
     CDS             complement(976..1740)
                     /locus_tag="POB55_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HDI6501334.1"
                     /translation="MTVEERSNTAKVDILGVDFDNTTMLQMVENIKTFFANQSTNNLF
                     IVTANPEIVNYATTHQAYLELINQASYIVADGTGVVKASHRLKQPLAHRIPGIELMDE
                     CLKIAHVNHQKVFLLGATNEVVEAAQYALQQRYPNISFAHHHGYIDLEDETVVKRIKL
                     FKPDYIFVGMGFPKQEEWIMTHENQFESTVMMGVGGSLEVFAGAKKRAPYIFRKLNIE
                     WIYRALIDWKRIGRLKSIPIFMYKIAKAKRKIKKAK"
     gene            1976..2728
                     /locus_tag="POB55_002395"
     CDS             1976..2728
                     /locus_tag="POB55_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HDI6501335.1"
                     /translation="MNYLSNFFNTTIQLNIYIIVIVAACYIAIHQYRHKPVLNYLDVI
                     LNYIPVLTHEFGHVLFNKLAGGRAKDLVIVTSPRERQQTLQQGFAITQSRHLAGQWLT
                     TIGGYLMPPIMLLIGLASSHYQIPSFFIFTYLLIFIYFLILTSRKGSPIVVITLISIM
                     LYFILKDENIVEIQLLVTMSYQYILGVLLGEVLQSSWTIAKLTFQRPSPQWDGSALKE
                     LSHVPIFIYSTIWIIFNLYAVNILLKYTHLIN"
     gene            complement(2808..3452)
                     /locus_tag="POB55_002396"
     CDS             complement(2808..3452)
                     /locus_tag="POB55_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HDI6501336.1"
                     /translation="MLTEEKEDYLKAILTNNGDKNFVTNKILSQFLNIKPPSVSEMVG
                     RLEKAGYVETKPYKGVRLTEDGLTHTLDIIKRHRLLELFLIEILKYNWEEVHQEAEIL
                     EHRISDLFVERLDSLLNFPETCPHGGVIPRNNEYKEKYITTILNYEPGDIVTIKRVRD
                     KTDLLIYLSSKDISIGNEVEIVSKDEMNKVIIIKRNDNVIIVSYENAMNMFAEK"
BASE COUNT         1140 a          596 c          506 g         1266 t
ORIGIN      
        1 caaaacacca actctgataa taacaaaatc ttctatacac tttacaacag gttttaaaat
       61 ttaacaactg ttgagtagta tattataatc tagataaatg tgaataagga aggtctacaa
      121 atgaacgttt cggtaaacat taaaaatgta acaaaagaat atcgtattta tcgtacaaat
      181 aaagaacgta tgaaagatgc gctcattccc aaacataaaa acaaaacatt tttcgcttta
      241 gatgacatta gtttaaaagc atatgaaggc gacgtcatag ggcttgttgg catcaatggt
      301 tccggcaaat caacgttgag caatatcatt ggcggttctt tgtcgcctac tgttggcaaa
      361 gtggatcgta atggtgaagt cagcgttatc gcaattagtg ctggcttgag tggacaactt
      421 acagggattg aaaatatcga atttaaaatg ttatgtatgg gctttaagcg aaaagaaatt
      481 aaagcgatga cacctaagat tattgaattt agtgaacttg gtgagtttat ttatcaacca
      541 gttaaaaagt attcaagtgg tatgcgtgca aaacttggtt tttcaattaa tatcacagtt
      601 aatccagata tcttagtcat tgacgaagct ttatctgtag gtgaccaaac ttttgcacaa
      661 aaatgtttag ataaaattta cgagtttaaa gagcaaaaca aaaccatctt tttcgttagt
      721 cataacttag gacaagtgag acaattttgt actaagattg cttggattga aggcggaaag
      781 ttaaaagatt acggtgaact tgatgatgta ttacctaaat atgaagcttt ccttaacgat
      841 tttaaaaaga aatccaaagc cgaacaaaaa gaatttagaa acaaactcga tgagtcccgc
      901 ttcgttatta aataaaccga aaaaaccgag aatctccatt taaggatttc ctcggtttta
      961 tttttgtcat catgattatt tcgccttttt tatttttctt tttgctttgg ctattttata
     1021 cataaatatt ggaatactct ttaatctacc aatacgtttc caatctatta atgctctata
     1081 tatccattca atgtttaatt ttctaaagat ataaggcgct ctctttttag ccccagcaaa
     1141 tacttcaaga gaaccaccta cgcccatcat cactgtagat tcaaattggt tttcatgtgt
     1201 cataatccat tcttcttgtt tagggaatcc catacctaca aatatgtaat caggtttaaa
     1261 cagtttaatt cgtttcacta ctgtctcatc ttctaaatca atataaccgt gatgatgtgc
     1321 aaacgatatg tttggatatc tttgttgcaa tgcatattgt gccgcttcta caacttcatt
     1381 agttgcccct agcaaaaata ctttttgatg atttacatga gcaattttca aacattcatc
     1441 catcaactca ataccaggta tacgatgcgc tagaggttgc tttaaacgat gcgaagcttt
     1501 gactactcct gtcccatcag caacaatata gctcgcttga tttattaact ctaaatacgc
     1561 ttgatgtgtc gtcgcgtaat tcactatttc agggttggct gttactataa aaagattatt
     1621 cgttgattga tttgcaaaaa aggttttaat attttcaacc atttgcaaca ttgttgtatt
     1681 atcaaaatcg actcctaaaa tgtcaacttt ggctgtattg gatctttctt caacagtcat
     1741 aacaggaatc tcctatctta tttcattatt atgttagtgt aatcaatatt aacagtattt
     1801 atattcatcc tatttatatc gaaatgattt tgctttattt taacacatat tcaccttttc
     1861 aaaaattaaa attagtttaa ctaaggtaag tctaaagtct aacgctattg atgccaatat
     1921 catttataat aagtgctaag caaactttaa attaatatct tatatgaggt taacaatgaa
     1981 ttatttatca aactttttta atacgacaat tcaacttaat atatacatta tcgttattgt
     2041 tgcagcttgt tatattgcta ttcatcaata tagacataag cctgttttga actatttaga
     2101 tgtcatttta aactatatcc ctgttttaac acatgaattt ggtcatgtac tgtttaataa
     2161 actcgctggt ggccgtgcaa aagatttagt cattgtaaca agtccacgag agcgacaaca
     2221 gacattacaa caaggattcg caattacaca atctagacat ctagcaggcc aatggcttac
     2281 aacgattggt ggttatttaa tgccacctat tatgttattg ataggtttag cgtcgagcca
     2341 ttatcagata cctagtttct ttatatttac ctatttattg atatttattt attttttaat
     2401 tttaacatct agaaaaggct cacccattgt tgttataact ttaatttcta tcatgttgta
     2461 ttttatatta aaagatgaaa atatagtaga aattcaacta cttgtcacaa tgagttatca
     2521 atatatttta ggtgtattat taggcgaagt tctacaatct agttggacga ttgctaaatt
     2581 gactttccag cgtccgagtc cacagtggga tggtagtgca cttaaagaac tcagtcatgt
     2641 acctatcttc atttatagta caatctggat tatcttcaat ctttatgcag tgaatatttt
     2701 actcaaatat actcacttga taaattaact aataaaaaag aagcctaaag caattatcca
     2761 tatgtcttta tcagttcaat catggatatc tttatggctt ctttatttta tttttcagca
     2821 aacatgttca ttgcattttc gtaactgaca ataattacat tatcattacg tttaatgata
     2881 attactttat tcatttcatc tttcgataca atttccactt cattaccaat agaaatatct
     2941 ttactagaca aatatattag caaatcggtc ttatctctca cacgtttgat tgtaacgata
     3001 tcaccaggtt cataattcaa aatcgttgtt atatatttct ctttatattc attatttcta
     3061 ggaatcacac cgccgtgcgg gcaagtttct gggaaattta acaggctatc cagcctttca
     3121 acaaataaat ctgaaattcg atgttctaaa atttctgctt cttgatgtac ttcttcccaa
     3181 ttatatttca atatttctat taaaaataat tctaatagtc gatgtctctt aatgatatca
     3241 agcgtatgcg ttaaaccatc ctctgttaat ctaacacctt tgtatggttt tgtttcaaca
     3301 tagcctgctt tttcaagacg tcctaccatt tcacttacag atggaggctt aatatttaaa
     3361 aattgagata agattttatt tgtcacaaag tttttatcgc cattattcgt aaggattgcc
     3421 tttaaatagt cctctttttc ttcagttaac atactttcac ctcacataca ttgtctatat
     3481 tatatcacga atttacttga catgataa
//
LOCUS       DAMZKC010000200         3506 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.203, whole genome shotgun sequence.
ACCESSION   DAMZKC010000200 DAMZKC010000000
VERSION     DAMZKC010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3506
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.203"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(91..1335)
                     /gene="fabF"
                     /locus_tag="POB55_002397"
     CDS             complement(91..1335)
                     /gene="fabF"
                     /locus_tag="POB55_002397"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HDI6501337.1"
                     /translation="MSQNKRVVITGMGALSPIGNDVKTTWENALKGVNGIDKITRIDT
                     EPYSVHLAGELKNFNIEDHIDKKEARRMDRFTQYAIVAAREAVKDAQLDINENTADRI
                     GVWIGSGIGGMETFEIAHKQLMDKGPRRVSPFFVPMLIPDMATGQVSIDLGAKGPNGA
                     TVTACATGTNSIGEAFKIVQRGDADAMITGGTEAPITHMAIAGFSASRALSTNDDIET
                     ACRPFQEGRDGFVMGEGAGILVIESLESAQARGANIYAEIVGYGTTGDAYHITAPAPE
                     GEGGSRAMQAAMDDAGIEPKDVQYLNAHGTSTPVGDLNEVKAIKNTFGEAAKHLKVSS
                     TKSMTGHLLGATGGIEAIFSALSIKDSKVAPTIHAVTPDPECDLDIVPNEAQDLDITY
                     AMSNSLGFGGHNAVLVFKKFEA"
     gene            complement(1347..2288)
                     /locus_tag="POB55_002398"
     CDS             complement(1347..2288)
                     /locus_tag="POB55_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HDI6501338.1"
                     /translation="MNVGIKGFGAYAPEKIIDNAYFEQFLDTSDEWISKMTGIKERHW
                     ADDDQDTSDLAYEASLKAIADAGIQPEDIDMIIVATATGDMPFPTVANMLQERLGTGK
                     VASMDQLAACSGFMYSMITAKQYVQSGDYHNILVVGADKLSKITDLTDRSTAVLFGDG
                     AGAVIIGEVSDGRGIISYEMGSDGTGGKHLYLDKDTGKLKMNGREVFKFAVRIMGDAS
                     TRVVEKANLTSDDIDLFIPHQANIRIMESARERLGISKDKMSVSVNKYGNTSAASIPL
                     SIDQELKNGKIKDDDTIVLVGFGGGLTWGAMTIKWGK"
     gene            2583..2768
                     /locus_tag="POB55_002399"
     CDS             2583..2768
                     /locus_tag="POB55_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HDI6501339.1"
                     /translation="MKHLVTFFWALLLMQMVNFVLNSLVGGDSLNVVNPIIMAVLFTI
                     FTAIFAAVIKPPKDSSQ"
     gene            complement(3025..3459)
                     /locus_tag="POB55_002400"
     CDS             complement(3025..3459)
                     /locus_tag="POB55_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAP domain-containing protein"
                     /protein_id="HDI6501340.1"
                     /translation="MKLKSFITVTLALGMIATTGATVAGNEVSAAEKDKLPATQKAKE
                     MQNVPYTIAVDGIMAFNQSYLNLPKDSQLSYLDLGNKVKALLYDERGVTPEKIRNAKS
                     AVYTITWKDGSKKEVDLKKDSYTANLFDSNSIKQIDINVKTK"
BASE COUNT         1064 a          689 c          515 g         1238 t
ORIGIN      
        1 attatgacga ttgtgctgtc gctcatctta gtataaagaa acagaaaaaa tcttcagtaa
       61 ctaaaacaac gttactgaag atttttataa ttatgcttca aatttcttga atactaatac
      121 tgcgttatgt ccaccgaatc ctaagctatt actcattgca taagtaatat caaggtcttg
      181 cgcttcattt ggaacaatat ccaaatcaca ttctggatct ggtgttaccg catgaattgt
      241 cggtgcgact ttagagtctt taattgaaag cgctgagaag attgcttcaa ttccacctgt
      301 tgcaccaagt aagtgaccag tcattgattt tgttgagcta acttttaagt gtttagctgc
      361 ttcaccaaat gtatttttaa tagctttaac ttcatttaag tcaccaacag gagtacttgt
      421 accatgggca tttaagtatt gtacatcttt aggttcaata ccagcatcat ccatagctgc
      481 ttgcattgct ctagaaccgc cttcaccttc tggagctggc gctgtaatat gataagcatc
      541 acctgtagta ccatagccaa ctatctcagc ataaatattg gcacctcgag cttgtgctga
      601 ttctaaagat tcaattacta aaataccagc accttcaccc ataacaaaac catctctacc
      661 ttcttggaat ggacgacatg ctgtttcaat gtcatcattt gtagaaagcg ctcgacttgc
      721 actgaaacca gcaattgcca tatgagtaat tggtgcttct gtaccaccag taatcattgc
      781 atctgcatca ccgcgttgca caattttaaa tgcttctccg attgaatttg tacctgttgc
      841 acatgctgta actgttgcac catttggtcc ttttgcacct aagtcaattg atacttgccc
      901 agttgccata tcaggaatta acattggtac gaaaaatgga ctcacacgtc ttgggccttt
      961 atccattaat tgtttatgtg caatttcaaa tgtttccata ccaccgatac cagaaccaat
     1021 ccatacaccg attcgatctg cagtattttc attgatatct aattgcgcat ctttaacagc
     1081 ctctctagct gctacaattg catattgagt aaatctatcc atacgacgcg cttctttttt
     1141 gtcgatatga tcttcaatat taaagttttt aagttctcct gctaagtgaa cgctataagg
     1201 ttcagtatcg atacgtgtaa ttttatcgat accatttacg ccttttagag cattctccca
     1261 tgttgttttg acatcattac cgattggaga aagggctccc atacctgtaa taactactct
     1321 tttattttga ctcattcgtt atcctcctat tttccccatt ttattgtcat tgcgccccaa
     1381 gttaggccgc caccgaatcc gacaagaaca attgtatcat catctttgat tttaccattt
     1441 tttaattctt gatcgatact taaaggtatt gacgcagctg aagtatttcc atatttattt
     1501 acagaaacac tcattttgtc ttttgaaata cctaagcgtt ctctagctga ttccataatt
     1561 ctaatattag cttgatgagg aataaataaa tctatatcat ctgatgttaa attcgctttt
     1621 tcaactacac gtgttgatgc atcacccata attctaacag caaatttaaa tacttctcga
     1681 ccattcattt tcagtttacc agtatcttta tctaaatata aatgtttacc acctgtgcca
     1741 tcagaaccca tttcataact tataatacct ctgccatctg aaacttcacc gatgataacc
     1801 gcacctgcac catctccaaa tagaactgca gtagaacggt cagttaaatc tgttatttta
     1861 gataatttat ctgcaccgac aactaaaatg ttatgataat ctccagattg aacatattgt
     1921 ttagctgtaa tcattgaata cataaatcca gaacatgctg caagttgatc catagaggca
     1981 actttgcccg tccctaaacg ttcttgcaac atatttgcga cagttggaaa tggcatatct
     2041 ccagttgctg tggcaacaat tatcatatct atatcttcgg gctgaatacc agcgtcagcg
     2101 attgctttta aacttgcttc atatgctaaa tctgaagtat cttgatcatc atctgcccaa
     2161 tgtctttctt taattccagt catcttagaa atccattcat cagatgtatc taaaaattgc
     2221 tcaaaatagg cattgtcaat aatcttttct ggcgcatatg caccaaaacc tttaataccc
     2281 acgttcatag ttgaacacct tcttaataaa atttttaata ccaggtatta atttaacata
     2341 gattactttt taaactcaag tcagaggtag gaatattgac tagttaataa aattttaatg
     2401 ctttattatc gtcaaatttc agtatgaaat tggttttaat gcgcttaatt tagaatagcc
     2461 attcagataa aatcatttat ttaaactgta taatatggtc aaaatatatg agatgttact
     2521 atacaaattt ctaactttca cttaaaataa aatatatata attaaagtgg aggtagaatg
     2581 acatgaaaca cctcgtaaca ttcttttggg cattattatt aatgcaaatg gttaactttg
     2641 tacttaatag cctagtaggt ggagacagct taaatgtagt gaatccaatt atcatggcag
     2701 tattgttcac gattttcaca gcaatttttg ctgcagtaat taaaccgccg aaagattcat
     2761 cacaataact atttagtata aaatgaaata tgtctttctt attattgaag ctgcccagat
     2821 aaaggcggct tttatttttg gccattaatg ttttgtattt tgaatatttt aaagctcaat
     2881 gacctctaac ccatcacttt taaatttaga aaaagcctta atacaaagat gtcatttacg
     2941 agtaatcttt gtatcaaggc aattatttta taatcatcac tgtaaaatta aatgcttcat
     3001 gttgaatttg ataattttaa gtgtttattt agtttttaca ttaatatcaa tttgtttaat
     3061 tgaatttgaa tcaaacaagt ttgctgtgta gctatctttc ttaagatcca cttctttttt
     3121 actaccatct ttccaagtaa tcgtgtaaac ggcagatttt gcatttcgaa tcttctcagg
     3181 tgttacaccg cgttcatcat ataacaaagc tttaacttta tttcctaaat ctaaatatga
     3241 taattggcta tcttttggta aatttaagta agattgatta aaagccataa tgccatctac
     3301 tgcaattgta tatggaacat tttgcatttc tttagctttt tgagttgccg gtagtttgtc
     3361 cttttctgct gcagatacct cattacctgc cacagtagcg ccagtcgttg cgatcatgcc
     3421 cagtgccaaa gttacagtta taaatgattt taatttcata taatctctct cctttgtgag
     3481 ttaaagtatc ttacaaaatc aatata
//
LOCUS       DAMZKC010000201         3467 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.204, whole genome shotgun sequence.
ACCESSION   DAMZKC010000201 DAMZKC010000000
VERSION     DAMZKC010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3467
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.204"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            59..448
                     /locus_tag="POB55_002401"
     CDS             59..448
                     /locus_tag="POB55_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YisL family protein"
                     /protein_id="HDI6501341.1"
                     /translation="MLHLHILSWVLAIILFIATYLNISKNQGGSPFFKPLHMILRLFM
                     LLTLISGFWILIQSFMNGGANHMLLTLKMLCGVAVVGLMEVSIAKRKRHEQSHKMFWI
                     TMALIIITMVLGVILPLGPISKLFGIG"
     gene            complement(620..1936)
                     /locus_tag="POB55_002402"
     CDS             complement(620..1936)
                     /locus_tag="POB55_002402"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HDI6501342.1"
                     /translation="MPKIVVVGAVAGGATCASQIRRLDKESDIIIFEKDRDMSFANCA
                     LPYVIGEVVEDRKYALAYTPEKFYDRKQITVKTYHEVIAINDERQTVTVLNRKTNEQF
                     EESYDKLILSPGASANSLGFESDITFTLRNLEDTDAIDQFIKANQVDKVLVVGAGYVS
                     LEVLENLYERGLHPTLIHRSDKINKLMDADMNQPILDELDKREIPYRLNEEIDAINGN
                     EITFKSGKVEHYDMIIEGVGTHPNSKFIESSNIKLDRKGFIPVNDKFETNVPNIYAIG
                     DIATSHYRHVDLPASVPLAWGAHRAASIVAEQIAGNDTIEFKGFLGNNIVKFFDYTFA
                     SVGVKPNELKQFDYKMVEVTQGAHANYYPGNSPLHLRVYYDTSNRQILRAAAVGKEGA
                     DKRIDVLSMAMMNQLTVDELTEFEVAYAPPYSHPKDLINMIGYKAK"
     gene            complement(1988..2812)
                     /locus_tag="POB55_002403"
     CDS             complement(1988..2812)
                     /locus_tag="POB55_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDI6501343.1"
                     /translation="MQPHLICLDLDGTLLNDNKEISSYTKQVLNELQQRGHQIMIATG
                     RPYRASQMYYHELNLTTPIVNFNGAYVHHPKDKNFKTCHEILDLGIAQNIIQGLQQYQ
                     VSNIIAEVKDYVFINNHDPRLFEGFSMGNPRIQTGNLLVHLKESPTSILIEAEESKIP
                     EIKNMLTHFYADHIEHRRWGAPFPVIEIVKLGINKARGIEQVRQFLNIDRNNIIAFGD
                     EDNDIEMIEYARHGVAMENGLQELKDVANNITFNNNEDGIGRYLNDFFNLNIRYYC"
     gene            2926..3234
                     /locus_tag="POB55_002404"
     CDS             2926..3234
                     /locus_tag="POB55_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HDI6501344.1"
                     /translation="MEEALKDSILGALEMVIDPELGIDIVNLGLVYKVNVDDEGVCTV
                     DMTLTSMGCPMGPQIIDQVKTVLAEIPEIQDTEVNIVWSPPWTKDMMSRYAKIALGVS
                     "
BASE COUNT         1088 a          560 c          589 g         1230 t
ORIGIN      
        1 attaatccca agaagcttta atttaataat ataaataatt aaagtggggg gaatcagtat
       61 gttacattta catatattaa gttgggtatt agcgattatt ttatttatcg ctacatactt
      121 aaacatttca aaaaatcaag gcggatcacc atttttcaaa ccgttgcaca tgattttacg
      181 cttatttatg ctgttgacgt taatttcagg attttggata ttaattcagt catttatgaa
      241 tggcggggca aatcatatgt tgcttacatt gaaaatgctg tgtggtgttg cagtagttgg
      301 attgatggaa gtgtcgattg ctaaaagaaa gagacatgaa caaagtcaca aaatgttttg
      361 gataacaatg gcattaatta tcatcacaat ggtattaggt gtcattctac cgttagggcc
      421 tatatcaaaa ttattcggta ttggctaatt gataagacat atagtatatg acataaggca
      481 tggacaatta ttagtccaag accttatgtc ttttttgatt ctaaaaatgt ttaaacataa
      541 aaaaaggact ggatagtagt accagtccaa cataattcta acctagacaa ttaagattag
      601 attgaatcgc atgattcatt tatttagctt tgtaaccaat catattgatt aaatctttag
      661 ggtggctata tggtggtgca taagccactt caaactcagt taactcatct acagttagct
      721 ggttcatcat tgccatcgat agtacatcaa tacgtttatc tgcaccttct tttcctactg
      781 cagctgctct taaaatctga cggtttgaag tgtcataata tacccttaag tgtaaagggg
      841 aatttcctgg gtaataattc gcgtgtgcac cttgagtgac ttccaccatt ttatagtcaa
      901 attgctttag ttcatttggt ttaacgccga cactcgcaaa tgtataatca aagaacttca
      961 caatattgtt gcctaagaag cctttgaatt caatagtgtc atttccagca atttgttcgg
     1021 caacaatact tgctgcacgg tgagcgcccc aagctaaagg aacactagcc ggtagatcga
     1081 catgtcgata atgtgatgtt gcaatatcgc ctattgcata aatgtttgga acatttgttt
     1141 caaatttatc gtttaccggt atgaaacctt ttcgatcaag tttgatattt gaactttcga
     1201 taaattttga attggggtga gtaccgacac cttcaataat catatcgtaa tgttcaactt
     1261 ttcctgattt aaatgtaatt tcatttccat tgatagcatc aatttcctca tttaaacggt
     1321 atggaatctc ccgcttatct aattcatcaa gtataggttg attcatgtca gcatccatta
     1381 atttatttat cttatcagat cgatgaatta aagtagggtg taaaccacgt tcgtaaagat
     1441 tttcaagaac ttctaatgaa acataccctg cacctacaac taatacttta tcaacttgat
     1501 ttgctttgat gaattgatcg atagcatcag tgtcttctaa atttctaagt gtaaatgtaa
     1561 tatcactttc aaagccaagg ctatttgcac ttgcaccagg gcttaaaatg agtttatcgt
     1621 aagattcttc aaattgttcg ttggtctttc tatttaatac agttacagtt tgtctttcat
     1681 cattgattgc aataacttca tgataagttt ttactgtaat ttgctttcta tcataaaatt
     1741 tttcaggtgt atacgctaaa gcatattttc tatcttcaac aacttcgcca atgacataag
     1801 gcaatgcaca attagcaaag ctcatatcac gatctttttc aaaaataata atgtcacttt
     1861 ctttatctaa acgtcgaatt tggctggcac atgttgcacc gccagcgact gctccgacta
     1921 cgactatttt gggcatcgta acttacctcc tattgtttta aaatattata aaatgattag
     1981 ttataaatta acagtaatat ctaatattta aattaaagaa atcattcaaa tatcgaccaa
     2041 tgccatcttc attattgttg aatgtaatat tgttcgctac atctttaagt tcttgcaaac
     2101 cattttccat agcaacacca tggcgggcgt actcaatcat ttcaatatca ttatcttcat
     2161 caccgaatgc aataatatta tttcggtcaa tatttaaaaa ttgtctaact tgctcaatgc
     2221 ctcttgcttt attaatacca agttttacaa tttcaatgac agggaatggt gcgccccagc
     2281 gtcgatgctc aatatgatcg gcataaaaat gagtaagcat atttttgatt tcaggtattt
     2341 tactttcttc ggcttcaatt aaaattgagg taggggattc tttcaagtgg acaagtaaat
     2401 taccagtttg aattcttgga ttacccattg aaaaaccttc aaataatctt ggatcatgat
     2461 tgttaatgaa aacataatct ttcacttctg ctataatatt cgatacttga tattgttgta
     2521 atccttgaat aatgttttgt gcgatgccta aatctaaaat ttcatggcaa gttttgaagt
     2581 ttttatcttt agggtgatgt acgtaagcgc cattaaaatt aacaattggt gtcgttaaat
     2641 ttaattcatg ataatacatt tgacttgcac gataaggtct gccagtcgca atcataattt
     2701 ggtgtccacg ttgttgtaat tcatttaata cttgtttagt atatgatgaa atttctttgt
     2761 tatcgtttaa taatgttccg tctaagtcta gacatattaa atgtggttgc atatctctct
     2821 cctcataagt tgcttagttc atatagatac atcatagcat tttattggcg ttttgtcgga
     2881 tatttgatat attatgaata atatgacatt aaagaggtga tatggatgga agaggcattg
     2941 aaagatagta tcttaggtgc attagaaatg gtaattgacc ctgaattagg aattgatatc
     3001 gttaatttag gtttagtata caaagtgaat gttgatgatg aaggcgtatg tacagttgat
     3061 atgactttaa catcaatggg atgtccaatg ggacctcaaa ttattgatca agttaaaaca
     3121 gtattagcag agattcctga aatacaggat actgaagtga atatcgtatg gagtccacct
     3181 tggacaaaag atatgatgtc acgttacgct aagattgcac ttggtgtgag ctaacataaa
     3241 cgtgcattgc ctgtagaatt tcttttagaa attctctgtg ttgggtcccc gcagtcaata
     3301 taacattgta gagtataatt cattgtgaat aagctccctt caaagtagac attgaaaaat
     3361 gaaaattttg aagggagcct tatttatgtt ctaggttctg atttcatttt gaaaatttat
     3421 atctaacttg tttcaaaaaa tatgaattaa gacagtgact tgcactt
//
LOCUS       DAMZKC010000202         3443 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.205, whole genome shotgun sequence.
ACCESSION   DAMZKC010000202 DAMZKC010000000
VERSION     DAMZKC010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3443
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.205"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..593
                     /gene="pmtB"
                     /locus_tag="POB55_002405"
     CDS             <1..593
                     /gene="pmtB"
                     /locus_tag="POB55_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     permease subunit PmtB"
                     /protein_id="HDI6501345.1"
                     /translation="MLDSTKFFYLLQSFISPTILIIWILDAGHLFRLNRRLGGNDSYY
                     FYMSLPVSKKQLLNANYITCIVLTLIGTLVISLYAYEADVIEPNSIYFSTAYAFVISN
                     FLSIPIAFSQFTELRRVKVPYGIYVFTIIILVPFLFSIAIVLVNYFLLSQSSFPDLYS
                     YILNIGFLIISIVILIVNYFKQLNKINTRKFKGGSR"
     gene            590..1462
                     /gene="pmtC"
                     /locus_tag="POB55_002406"
     CDS             590..1462
                     /gene="pmtC"
                     /locus_tag="POB55_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     ATP-binding protein PmtC"
                     /protein_id="HDI6501346.1"
                     /translation="MKLEHITKKYGSNVVLNDIDFDFGDSRIVGLIGKNGVGKTTVMK
                     VMNGNIIKFDGKVDIDNADNIGFLIEHPKLYDNKSGLYNLKLFAQVLGKGFDKAYTDK
                     IIDAFGMRPYIKKKVKKYSMGMKQKLAIAVSLMNKPKFLILDEPTNGMDPDGSIDVLT
                     TIKSLVNELDMRILISSHKLEDIELICDRAVFLRDGHFVQDVNMEEGVASDTTIVTVD
                     HKDFDRTEKYLAEHFQLQNVDKADGHLMINAQKNYQVILKALSELDIYPKYIETRKSS
                     LRDTYFNINQRGDK"
     gene            1462..2202
                     /gene="pmtD"
                     /locus_tag="POB55_002407"
     CDS             1462..2202
                     /gene="pmtD"
                     /locus_tag="POB55_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     permease subunit PmtD"
                     /protein_id="HDI6501347.1"
                     /translation="MRILNLVKYDLYSIFKSPLTYLAILVVSSLIATQSILMANSMDN
                     PKHIIVYGSVFAAAKWLLLIIGLMFVVKTITRDFSQGTIQLYMSKVKTRVGYIISKTI
                     SIILISILFALIHYVILIVVQASSNGKNLAFSKYVDNLWFFLIFLLFFGLFLFLITLA
                     SQKTAMIFSLGVFLVLIVPFIKPFITFIPRYGEKVLDAFDYIPFAYLTDKMISSNFDF
                     SNWQWVISLGSIVIFFILNILYVAKKDI"
     gene            complement(2268..2831)
                     /locus_tag="POB55_002408"
     CDS             complement(2268..2831)
                     /locus_tag="POB55_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDI6501348.1"
                     /translation="MTNLETYFKNSQPLNEYIDGMKTNQDTVLSIYQSFELPEDDSRI
                     KKIKEMNYSKVLVITEDWCGDAMMNLPILKHISEALNLEVRVFHRDDDTKLIDQYLTN
                     GKSRAIPIFVFLNDQFEQETVWGPRANEVQKFVTDVRADKLPSKDHPDYADLEKETHL
                     VISNRYKTDASFWKAVYNSILNKLENK"
     gene            2952..>3443
                     /locus_tag="POB55_002409"
     CDS             2952..>3443
                     /locus_tag="POB55_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501349.1"
                     /translation="MLYLNKYKERVTSVRIQNRWVVFILFLICSFGVLIGLYQYRHTK
                     TVDLSNLEINDIKLNEKFDKKGYEVNKKIKFDRFKFYNSKAHPDLTVKVREKDNIVKG
                     IILVRDEKIHTNFDGGIGSPINNAIENLGFGCKRTKVGNDFSSVKYIDRDNHLKLNLL
                     YQDL"
BASE COUNT         1177 a          430 c          550 g         1286 t
ORIGIN      
        1 taatgcttga ctcaacaaag ttcttttacc tattgcaatc ttttataagt ccaacaatat
       61 tgattatttg gattttagat gctggtcact tatttagact caatcgtaga ttaggtggta
      121 atgattcata ttatttttat atgagtttac cagtgtcaaa aaaacaattg cttaatgcca
      181 actatattac atgcattgtt ttaacattaa ttggaacgct tgttattagt ttatatgctt
      241 atgaagcaga tgtgattgaa ccaaattcaa tctatttttc aactgcatat gcatttgtca
      301 tatctaactt cttgtctata ccaattgcat ttagtcaatt tacagaattg cgtagagtca
      361 aagtgccata tggtatatac gtgtttacta ttatcatttt agttccattt ttattttcaa
      421 ttgcaatagt gttggtgaat tattttcttc taagtcaatc atcattccca gatttatatt
      481 catatatttt aaatattggt tttctaatta taagcattgt tatacttatt gttaattatt
      541 ttaaacagct caataaaata aatactagaa aatttaaagg aggcagtcga tgaaattaga
      601 acatattaca aaaaaatacg gctcaaatgt cgttttaaat gatattgatt ttgactttgg
      661 cgatagtaga attgtcggat taataggaaa aaacggtgtt ggtaaaacaa ccgttatgaa
      721 agtaatgaat ggtaatatta ttaaatttga tggaaaagta gacattgata atgcagataa
      781 tatcggtttt ttaattgagc atcctaaatt atatgataat aaatcaggat tgtataactt
      841 gaaattattt gcacaagtat taggtaaggg ttttgataaa gcatacacag acaaaattat
      901 agatgcattt ggtatgagac cttatattaa aaagaaagtt aagaaatatt caatggggat
      961 gaagcaaaag ttagcaattg cagtatcttt aatgaataaa cctaaatttt taatcttgga
     1021 tgagcctaca aatggtatgg atccagatgg ctcaattgat gtgctgacta caattaagtc
     1081 tctagtaaat gaacttgata tgagaattct aatatcaagt cataagttag aagatattga
     1141 attaatttgt gatagagctg tatttttaag agacggacat tttgttcaag atgtaaacat
     1201 ggaggaaggt gttgcatctg acacaacgat agttactgtt gatcataaag actttgatag
     1261 aactgaaaaa tatcttgcag agcatttcca attacaaaat gtcgacaaag cagacggaca
     1321 tttaatgatt aatgcacaaa aaaattatca agttatacta aaagcattat ctgaattaga
     1381 tatttatccg aaatatattg aaacacgtaa aagttcattg cgtgatacgt acttcaatat
     1441 aaatcaaaga ggtgataaat aatgagaatt ttaaatttag ttaagtatga tctttatagt
     1501 atttttaaaa gtcctttaac atatttagcg atactagtcg tatctagttt gattgcaact
     1561 caaagtatac ttatggcaaa ttctatggat aacccgaaac atattattgt ctatggatct
     1621 gtatttgctg cagcaaaatg gttattgtta ataattggat taatgtttgt tgttaagaca
     1681 attacgcgtg atttttcaca aggtacaatt caactatata tgagtaaagt taaaacacgc
     1741 gttggataca ttatttcgaa aacaatttca attattttaa tttcaatatt atttgcatta
     1801 attcattatg tgattttgat tgttgtgcag gcatctagta atggaaaaaa tttggcgttt
     1861 tctaaatatg tagataattt atggttcttc ctaatctttt tactattctt tggcttgttt
     1921 ttattcttaa tcacacttgc atcacaaaaa acagcaatga tattttcatt aggtgtattt
     1981 ttagtactta ttgtaccgtt tattaaacct tttattacat ttatcccaag atacggtgaa
     2041 aaagttttag atgcttttga ttatatccct tttgcttact taactgataa aatgattagc
     2101 tctaactttg attttagcaa ttggcaatgg gtaatttcat taggttctat agtaatattc
     2161 ttcattttga atatcttata tgtcgctaaa aaagacattt aataaaaata attttgaggt
     2221 tgggaatttt aaattttccc aacctcaaag tttgtcttat tgtaaattta tttattttct
     2281 aatttattta ggatggaatt ataaactgct ttccaaaatg aagcgtcagt tttatagcgg
     2341 tttgatataa ctaagtgtgt ttctttttct aaatctgcat agtctggatg atctttgctc
     2401 ggtaatttat cagcacgaac atcagttaca aatttttgga cttcatttgc tcttggtccc
     2461 caaactgttt cttgttcgaa ttgatcattc aagaatacga agataggaat tgcacgtgat
     2521 ttaccatttg ttaaatattg atcgatcagt tttgtatcat catctctatg gaacacgcgt
     2581 acttctaaat ttaatgcttc actgatgtgt tttagaattg ggagattcat cattgcatct
     2641 ccacaccagt cttcagtaat tactaatact ttagaataat tcatctcttt tatttttttg
     2701 atgcgtgaat catcttctgg taactcaaat gattgataga tactgagaac ggtatcttga
     2761 tttgtcttca ttccatcaat gtattcattt aagggttggc tatttttgaa ataagtttct
     2821 aaatttgtca ttgtaaaaac ctcctttagc atttacaaca ttataccaat ttatagtaat
     2881 aaaaggtaat gaaataaatt aattgcaaat tctttgttaa tttttgttaa ggatgaaacg
     2941 ggaagcacct tatgctatat ttaaataagt acaaagaaag ggtgacatca gtgcgtattc
     3001 aaaatcgctg ggttgtgttt atattatttt taatctgttc ttttggtgta ttaattggtt
     3061 tatatcaata tcgtcatacg aaaactgtgg atttgtctaa tcttgaaata aacgatatta
     3121 aattaaatga gaagtttgat aaaaaaggct atgaagtaaa taaaaaaatt aagtttgatc
     3181 gttttaaatt ttataacagt aaagctcacc ctgatcttac cgttaaagtg agagaaaagg
     3241 ataacatcgt taaggggata atattagtaa gagatgaaaa gatacatact aattttgatg
     3301 ggggaattgg ttcgccgata aataacgcga ttgaaaatct tggattcgga tgtaaaagaa
     3361 caaaagttgg caatgatttc tcatcggtaa agtatattga tagagataac catttaaaat
     3421 taaacttact ttatcaagat tta
//
LOCUS       DAMZKC010000203         3388 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.206, whole genome shotgun sequence.
ACCESSION   DAMZKC010000203 DAMZKC010000000
VERSION     DAMZKC010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.206"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(38..739)
                     /locus_tag="POB55_002410"
     CDS             complement(38..739)
                     /locus_tag="POB55_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6501350.1"
                     /translation="MIELKHVTFGYNKKQMVLQDINITIPDGENVGILGESGCGKSTL
                     ASLVLGLFKPAKGEIYLSDNAVLPIFQHPLTSFNPDWTIETSLKEALYYYRGLTDNTA
                     QDQLLLQHLSTFELNAQLLTKLPSEVSGGQLQRFNVMRSLLAQPRVLICDEITSNLDV
                     IAEQNVINILKAQTITNLNHFIVISHDLSVLQRLVNRIIVLKDGMIVDDFAIEELFNV
                     DRHPYTKELVQAFSY"
     gene            complement(732..1505)
                     /locus_tag="POB55_002411"
     CDS             complement(732..1505)
                     /locus_tag="POB55_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6501351.1"
                     /translation="MSLIDIQNLTIKNTSEKSLIKGIDLKIFSQQINALIGESGAGKS
                     LIAKALLEYLPFDLSCTYDSYQFDGENVSRLSQYYGHTIGYISQNYAESFNDHTKLGK
                     QLTAIYRKHYKGSKEEALSKVDKALSWVNLQSKDILNKYSFQLSGGQLERVYIASVLM
                     LEPKLIIADEPVASLDALNGNQVMDLLQHIVLEHGQTLFIITHNLSHVLKYCQYIYVL
                     KEGQIIERGNINHFKYEHLHPYTERLIKYRTQLKRDYYD"
     gene            complement(1492..2322)
                     /locus_tag="POB55_002412"
     CDS             complement(1492..2322)
                     /locus_tag="POB55_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6501352.1"
                     /translation="MHKIFSKNNLIFFVFVAFIFVVIVLQFFVSSENATKVNLSQTFE
                     PISWLHLLGTDDYGRDLFTRIIIGARSTLFVTVLTLIAIVVIGVTLGLFAGYKKGWIE
                     RLVLRFIDVGLSIPEFIIMIALASFFQPSLWNLVISITLIKWMNYTRLTRSIVNSEMN
                     KPYIKMAQLFHVPTRTILIRHLTPKIIPAIIVLMVVDFGKIILYISSLSFIGLGAQPP
                     TPEWGAMLQQGRDFISSHPIMLIAPASVIAITILIFNLTGDALRDRLLKQRGEYDESH
                     "
     gene            complement(2315..3301)
                     /locus_tag="POB55_002413"
     CDS             complement(2315..3301)
                     /locus_tag="POB55_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6501353.1"
                     /translation="MFIIKSMLYRLMQMIVVLFVISTLTFILMKLSPGNPVDKILHLD
                     VAQVSTEQINATKDKLGLNDSLLVQWWHWMNHLLHFNLGKSFESKEPVTQILFNYAPI
                     TLLISFSTLVVSLCISIPLGIIAAKRFHKWTDKVIRVISTLSISLPAFFIGIILLFIV
                     TNLMNIDSVILSQFILPVITLSLGMCAYIIRLVRSNLLMLLQSNIVQASRLRGMNERY
                     ILIHDLLKPTILPIIPLLGISLGSLIGGTVVIENLFDIPGIGYLLMDSIKSRDYPVIQ
                     GCVLFIGFFVVIINTIADLLTLLLDPKQRLQLGNPKIKTNTPLISVSSDRHA"
BASE COUNT         1183 a          601 c          484 g         1120 t
ORIGIN      
        1 tttataacaa gtctagaatt atcgcattct taaatagcta atatgaaaat gcttgcacta
       61 attcttttgt ataagggtgt ctatcaacat taaataattc ctctattgca aaatcatcga
      121 ctatcatgcc atccttaaga acgataattc tattaactaa gcgttgtaac acggataaat
      181 catgagaaat aacgataaaa tgatttaagt tcgtaatcgt ttgcgctttt aatatattta
      241 ttacattttg ttcagctata acatctaaat ttgaagttat ctcatcacat attaaaacgc
      301 gaggctgtgc taataacgaa cgcatgacat taaatctttg taattgtccg ccactcactt
      361 cgcttggtaa tttagtcaat aattgcgcgt ttaactcaaa agtagataaa tgttgtaata
      421 ataattgatc ctgagcagta ttatcagtta ggcctctgta ataatataac gcttctttta
      481 atgaggtctc aatcgtccaa tcagggttaa agctagttaa agggtgttgg aaaatcggta
      541 acacagcatt gtcacttaag taaatttctc ctttagcagg tttaaacaag ccaagaacca
      601 atgaagcgag cgtactttta ccacagccac tttcgcctaa aataccaaca ttttctccat
      661 caggtatagt aatattgata tcttgtagca ccatctgctt tttattataa ccaaaagtca
      721 catgttttaa ctcaatcata gtaatccctc tttaattgtg ttctatattt aattagacgt
      781 tcagtatacg gatgcaaatg ctcatacttg aaatgattaa tattacctcg ttcaatgatt
      841 tgaccttctt ttaaaacata aatgtactga caatatttca atacatgact taagttatgt
      901 gtgataataa ataatgtttg accatgttct aatacaatat gctgtaataa atccatcact
      961 tgattaccgt tcaaagcatc caatgatgca actggttcgt ctgcaatgat taatttaggc
     1021 tccaacatga gaacgcttgc tatgtatacg cgttcaagtt ggcccccaga aagttggaaa
     1081 ctatatttat ttaatatatc tttgctttgt aaattaaccc acgacaaagc cttatcaact
     1141 ttggacaaag cctcttcttt actaccttta taatgcttac gataaatcgc agttaactgt
     1201 ttacctaatt tagtatggtc gttaaaactt tctgcataat tttgagaaat atagccaatt
     1261 gtatgaccat aatattgact caatctacta acattttccc catcaaattg gtacgaatca
     1321 tacgtgcagc ttaaatcaaa tggtaaatat tcaagtaaag ctttagcaat caaacttttt
     1381 ccagcgccgc tctctccaat caaggcatta atctgttgac taaaaatttt caaatcaatc
     1441 cctttaataa gagatttctc actagtattc tttattgtta aattttgtat atcaatgaga
     1501 ctcatcatat tcaccccgtt gtttcagcaa tctatctctt agtgcatcac cggttaaatt
     1561 aaaaattaaa atagttatag caatgactga agcaggtgca atcaacataa ttggatgaga
     1621 cgaaataaaa tcacgacctt gttgcaacat agcgccccac tctggtgttg gcggttgtgc
     1681 acctaaccca ataaatgata gtgaacttat atatagaatg attttaccga aatcaacgac
     1741 catcaaaacg ataatagccg gtataatttt aggtgttaaa tgacgtatta atattgttct
     1801 tgttggtaca tgaaataatt gtgccatttt tatataaggc ttattcattt cgctattaac
     1861 tatacttcta gtcaaccttg tgtaattcat ccattttatt aatgtaattg agataactaa
     1921 attccataaa gatggttgaa aaaaacttgc taaagcaatc atgatgataa attctggaat
     1981 actcagacca acatcaataa accttaacac taatcgttca atccaccctt ttttatatcc
     2041 ggcaaataga cctagtgtaa cacctatgac aacgatagct attaatgtta aaacagtaac
     2101 aaacaatgtt gaacgtgcac cgataataat tcgggtaaat aaatctctcc cataatcatc
     2161 agttcctaat aaatgcaacc aactaatagg ttcaaaagtt tgtgataaat tgactttggt
     2221 tgcattttca ctactgacaa agaattgcag tacaattacc acaaaaataa atgcaacgaa
     2281 tacaaaaaat atcaggttat tctttgaaaa tattttatgc atgacggtca ctacttactg
     2341 atatcaatgg tgtattggtt ttgatttttg gatttcctaa ttgtaaacgc tgcttcggat
     2401 caagtaataa cgttaataaa tcagcaatcg tattgataat aacaacgaag aagccaataa
     2461 ataacacgca tccttgaata acaggataat ctcgagattt aatactatcc attaatagat
     2521 aaccaatacc aggtatatca aataaatttt caatcactac agtaccacct attagactgc
     2581 caagtgaaat ccctagtaat gggataatcg gcaaaattgt tggttttagt aaatcatgaa
     2641 ttaaaatata acgttcattc ataccgcgta atcttgatgc ttgtacgata ttactttgca
     2701 ataacatcaa taaattagaa cgcactaaac gaatgatgta tgcacacata cctaaagata
     2761 gcgtgattac aggtaatata aactgactta gtataacgct atctatattc attaaatttg
     2821 tgacaataaa taataaaata ataccgataa agaacgctgg taaactaatc gatagtgttg
     2881 agatcactct aatcacttta tccgtccact tatgaaatcg tttggctgct ataatgccga
     2941 gcggtataga tatgcataac gacactacta atgttgaaaa tgatatgagt aatgttatgg
     3001 gtgcatagtt gaataatatc tgtgttaccg gttcttttga ttcaaaactt tttcctaaat
     3061 taaaatgtaa taaatgattc atccaatgcc accactgtac caataaagaa tcatttaatc
     3121 ccaatttatc tttggttgca tttatttgtt ccgtcgacac ttgtgctaca tcaagatgta
     3181 atattttatc aacaggattg cctggtgata atttcattaa aatgaatgta agtgtagaaa
     3241 taacaaataa aacaactatc atttgcatca gtctatacaa catagacttt attatgaaca
     3301 taatagtccc cctccttgtg taagttacta acactttctt tttacatgag aatggcgcat
     3361 gtatatgcaa cttacatatt aagaacca
//
LOCUS       DAMZKC010000204         3371 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.207, whole genome shotgun sequence.
ACCESSION   DAMZKC010000204 DAMZKC010000000
VERSION     DAMZKC010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3371
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.207"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            45..>3371
                     /locus_tag="POB55_002414"
     CDS             45..>3371
                     /locus_tag="POB55_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HDI6501354.1"
                     /translation="MLNRENKTAITRKGMVSNRLNKFSIRKYTVGTASILVGTTLIFG
                     LGNQEAKAAESTNKELNEATTSASDNQSSDKVDMQQLNQEDNTKNDNQKEMVSSQGNE
                     TTSNGNKSIEKESVQSTTGNKVEVSTAKSDEQASPKSTNEDLNTKQTISNQEALQPDL
                     QENKSVVNAQPTNEENKKVDAKTESTTLNVKSDAIKSNAETLVDNNSNSNNENNADII
                     LPKSTAPKRLNTRMRIAAVQPSSTEAKNVNDLITSNTTLTVVDADKNNKIVPAQDYLE
                     LKSQIKVDDKVKSGDYFTIKYSDTVQVYGLNPEDIKNIGDIKDPNNGETIATAKHDTA
                     NNLITYTFTDYVDRFNSVQMGINYSIYMDADTIPVSKNDVEFNVTIGNTTTKTTANIQ
                     YPDYVVNEKNSIGSAFTETVSHVGNKENPGYYKQTIYVNPSENSLTNAKLKVQAYHSS
                     YPNNIGQINKEVTDIKIYQVPKGYTLNKGYDVNTKELTDVTNQYLQKITYGDNNSAVI
                     DFGNADSAYVVMVNTKFQYTTSESPTLVQMVTLSSNNSKSASMGNALGFTNNQSGGAG
                     QEVYKIGNYVWEDTNKNGVQELGEKGVGNVTVTVFDNNTNTKVGEAVTKEDGSYLIPN
                     LPNGDYRVEFSNLPKGYEVTPSKQGNNEELDSNGLSSVITVNGKDNLSADLGIYKPKY
                     NLGDYVWEDTNKNGIQDQDEKGISGVTVTLKDENGNVLKTVTTDADGKYKFTDLDNGN
                     YKVEFTTPEGYTPTTVTSGSDIEKDSNGLTTTGVINGADNMTLDSGFYKTPKYNLGNY
                     VWEDTNKDGKQDSTEKGISGVTVTLKNENGEVLQTTKTDKDGKYQFTGLENGTYKVEF
                     ETPSGYTPTQVGSGTDEGIDSNGTSTTGVIKDKDNDTIDSGFYKPTYNLGDYVWEDTN
                     KNGVQDKDEKGISGVTVTLKDENDKVLKTVTTDENGKYQFTDLNNGTYKVEFETPSGY
                     TPTSVTSGNDTEKDSNGLTTTGVIKDADNMTLDSGFYKTPKYSLGDYVWYDSNKDGKQ
                     DSTEKGIKDVKVTLLNEKGEVIGTTKTDENGKYRFDNLDSGKYKVIFEKPAGLTQTGT
                     NTTEDDKDADGGEVDVTITDH"
BASE COUNT         1426 a          471 c          590 g          884 t
ORIGIN      
        1 aacaattgta ttatttaaaa atttgatgaa ttaggagtaa tctaatgcta aacagagaaa
       61 ataaaacggc aataacaaga aaaggcatgg tatccaatcg attaaataaa ttttcgatta
      121 gaaagtacac agtgggaaca gcatcaattt tagtaggtac aacattaatt tttggtctgg
      181 ggaaccaaga agcaaaggct gcagaaagta ctaataaaga attgaacgaa gcgacaactt
      241 cagcaagtga taatcaatcg agtgataaag ttgatatgca gcaactaaat caagaagaca
      301 atactaaaaa tgataatcaa aaagaaatgg tatcatctca aggtaatgaa acgacttcaa
      361 atgggaataa atcaatagaa aaagaaagtg tacaatctac cactggaaat aaagttgaag
      421 tttcaactgc caaatcagat gagcaagctt caccaaaatc tacgaatgaa gatttaaaca
      481 ctaaacaaac tataagtaat caagaagcgt tacaacctga tttgcaagag aataaatcag
      541 tggtaaatgc tcaaccaact aatgaggaaa acaaaaaggt agatgccaaa actgaatcaa
      601 ctacattaaa tgttaaaagt gatgctatca agagtaatgc tgaaactctt gttgataaca
      661 atagtaattc aaataatgaa aataatgcag atatcatttt gccaaaaagt acagcaccta
      721 aacgtttgaa tacaagaatg cgtatagcag cagtacagcc atcatcaaca gaggctaaaa
      781 atgttaatga tttaattaca tcaaatacaa cattaactgt cgttgatgca gataaaaaca
      841 ataaaatcgt accagcacaa gattatctag aattaaaatc acaaattaaa gttgatgaca
      901 aagttaaatc aggtgattat ttcacaataa aatactcaga tacagtacaa gtatatggat
      961 tgaatccgga agatattaaa aatattggag atattaaaga tccaaataat ggtgaaacaa
     1021 ttgcgactgc aaaacatgat actgcaaata atttaattac atatacattt acagattatg
     1081 tcgatagatt taattctgta caaatgggaa ttaattattc aatttatatg gatgctgata
     1141 caattcctgt tagtaaaaac gatgttgagt ttaatgttac gataggtaat actacaacaa
     1201 aaacaactgc taacattcaa tatccagatt atgttgtaaa tgagaaaaat tcaattggat
     1261 cagcgttcac tgaaacagtt tcacatgttg gaaataaaga aaatccaggg tactataaac
     1321 aaacgattta tgtaaatcca tcggaaaatt ctttaacaaa tgccaaacta aaagttcaag
     1381 cttaccactc aagttatcct aataatatcg ggcaaataaa taaagaggta acagatataa
     1441 aaatatatca agttcctaaa ggttatacat taaataaagg atacgatgtg aatactaaag
     1501 agcttacaga tgtaacaaat caatacttgc agaaaattac atatggcgac aacaatagcg
     1561 ctgttattga ttttggaaat gcagattctg cttatgttgt aatggttaat acaaaattcc
     1621 aatatacaac tagcgaaagc ccaacacttg ttcaaatggt tacattaagt tcaaacaata
     1681 gtaaatctgc ctcaatgggt aatgctttag gattcactaa taaccaaagt ggcggagctg
     1741 gtcaagaagt atataaaatt ggtaactacg tatgggaaga tactaataaa aacggtgttc
     1801 aagaattagg agaaaaaggc gttggcaatg taactgtaac tgtatttgat aataatacaa
     1861 atacaaaagt aggagaagca gttactaaag aagatgggtc atacttgatt ccaaacttac
     1921 ctaatggaga ttaccgtgta gaattttcaa acttaccaaa aggttatgaa gtaacccctt
     1981 caaaacaagg taataacgaa gaattagatt caaacggctt atcttcagtt attacagtta
     2041 atggcaaaga taacttatct gcagacttag gtatttacaa acctaaatac aacttaggtg
     2101 actatgtctg ggaagataca aataaaaatg gtatccaaga ccaagatgaa aaaggtatat
     2161 ctggcgtaac ggtaacatta aaagatgaaa acggtaacgt gttaaaaaca gttacaacag
     2221 acgcagatgg caaatataaa tttactgatt tagataatgg taattataaa gttgaattta
     2281 ctacaccaga aggctataca ccgactacag taacatctgg tagcgacatt gaaaaagact
     2341 ctaatggttt aacaacaaca ggtgttatta atggtgctga taacatgaca ttagatagtg
     2401 gattctacaa aacaccaaaa tataatttag gtaattatgt atgggaagat acaaataaag
     2461 atggtaagca ggattcaact gaaaaaggta tttcaggcgt aacagttaca ttgaaaaatg
     2521 aaaacggtga agttttacaa acaactaaaa cagataaaga tggtaaatat caatttactg
     2581 gattagaaaa tggaacttat aaagttgaat tcgaaacacc atcaggttac acaccaacac
     2641 aagtaggttc aggaactgat gaaggtatag attcaaatgg tacatcaaca acaggtgtca
     2701 ttaaagataa agataacgat actattgact ctggtttcta caaaccgact tacaacttag
     2761 gtgactatgt atgggaagat acaaataaaa acggtgttca agataaagat gaaaagggta
     2821 tttcaggtgt aacagttacg ttaaaagatg aaaacgacaa agttttaaaa acagttacaa
     2881 cagatgaaaa tggtaaatat caattcactg atttaaacaa tggaacttat aaagttgaat
     2941 tcgagacacc atcaggttat acaccaactt cagtaacttc tggaaatgat actgaaaaag
     3001 attctaatgg tttaacaaca acaggtgtca ttaaagatgc agataacatg acattagaca
     3061 gtggtttcta taaaacacca aaatatagtt taggtgatta tgtttggtac gacagtaata
     3121 aagacggcaa acaagattca actgaaaaag gtatcaaaga tgttaaagtt actttattaa
     3181 atgaaaaagg cgaagtaatt ggaacaacta aaacagatga aaatggtaaa tatcgtttcg
     3241 ataatttaga tagcggtaaa tacaaagtta tttttgaaaa gcctgctggc ttaacacaaa
     3301 caggtacaaa tacaactgaa gatgataaag atgcagatgg tggcgaagtt gacgtaacaa
     3361 ttacggatca t
//
LOCUS       DAMZKC010000205         3363 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.208, whole genome shotgun sequence.
ACCESSION   DAMZKC010000205 DAMZKC010000000
VERSION     DAMZKC010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3363
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.208"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            519..1211
                     /locus_tag="POB55_002415"
     CDS             519..1211
                     /locus_tag="POB55_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL11"
                     /protein_id="HDI6501355.1"
                     /translation="MKLKNIAKASLALGILTTGMITTTAQPVKAIEQSRLSVTSKDTQ
                     ELKKYYSGTGYNFQNVSGYREGNKMNIIDGPQLNVVTLLGTDKERFKDDEDYEGLDVF
                     VVREGSGKHADNISIGGITKTNKNQYKDPVQNVNLLTSKSNGQNTASVTSEYYSINKE
                     EISLKELDFKLRKQLIDKHDLYKTEPKDSKIKVSMKNGGYYTFELNKKLQPHRMGDTI
                     DSRNIKKIEVNL"
     gene            1233..2735
                     /locus_tag="POB55_002416"
     CDS             1233..2735
                     /locus_tag="POB55_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKLRK protein"
                     /protein_id="HDI6501356.1"
                     /translation="MRENFKLRKMKVGLVSVAITMLYIMTNGEAEASEGSQTVKNPKV
                     NATEEIKVGSQPVQNNQEVSSEQTKKNFVNLDPIKPGAQKVTGTTLPSHIILMNIDGK
                     SADSVDGGNSDLVFADENGRFEYPLHNRKIVHNQEIEVSSSSPDLGDDEEDEEVEEDS
                     TEKAGTEEENTDAKATYTTPRYEKAYEIPKEQLKEKDGHHQVFIEPITEGSGIIKGHT
                     SVKGKVALSINNKFINFEERAKDGISKEDTKASSDGVWMPINEKGYFDFDFKKNPFDN
                     LELKKNDEISLTFAPDDEDEALKSLIFKTKVTSLEDIDKAETKYDHTKVEKVKVLKDV
                     KEDLHVDEIYGSLYHTEQGKGILDKQGTKEITGKTKFANAVVKVYSDLGDAQLFPDIQ
                     VDENGKFSFDAEKAGFRLQNGETLNFAVVKPITGELLHQGFVSKYIDVYESPEEKKER
                     EFEEKLENTPAYHKLHGDKIVGYDVQGNPSTWFYPLGEKKVERKAPKLEK"
     gene            complement(2842..3150)
                     /gene="spn"
                     /locus_tag="POB55_002417"
     CDS             complement(2842..3150)
                     /gene="spn"
                     /locus_tag="POB55_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myeloperoxidase inhibitor SPIN"
                     /protein_id="HDI6501357.1"
                     /translation="MKFKKVLVATAMVGVLATGVVGYSNQADAKVYSQNGLVLHDDAN
                     FLEHELSYIDVLLDKNADQATKDNLRSYFADKGLHSIKDIINKAKQDGFDVSKYEHVK
                     "
BASE COUNT         1326 a          423 c          602 g         1012 t
ORIGIN      
        1 agaaaataag agggtttcta gaagttttat ctggaataac tactaaacaa ttgcacaaga
       61 tagaccaatt aaaagagagg aaaaaggcgt ttttacagaa aatgtttatt tgatttgtcg
      121 ctgcaatata gtttttatta tttgtttatt tcagatgttt caccatcata ttgcgtaact
      181 tttacaaata agaaataaag ttcaatgaaa tcaaaaacga acattaaatt taggcactgt
      241 gatagcacag tgtctttttt gtgtcgaaat tgtgtacaga ataagtagtt aaataaagat
      301 taagttgaga taaagtgtta ttcgtaaata aaagagagta gatcgatagg aattgaatga
      361 tattagttaa ctatttatta aattacttaa taatgattaa tttttagtta aagtaagttt
      421 aatgtgaagc acgaccattg ctcattataa tgaatgagga ttgttcgtat tgcgtaatag
      481 aataaatcaa atagactaaa aattgggagc atagaattat gaaattaaaa aatattgcta
      541 aagcaagttt agcactaggg attttaacaa cagggatgat tacaactact gctcagccag
      601 taaaagcaat tgagcaaagc agattatcag ttacttcaaa agatacacaa gaattaaaaa
      661 aatactacag tggaacagga tataattttc aaaatgtgag tggttataga gaaggtaata
      721 aaatgaacat tattgatgga ccacaactta atgtagttac tttacttggc acagacaaag
      781 aaaggtttaa ggacgatgaa gattatgaag gacttgatgt atttgttgta agagaagggt
      841 caggtaaaca cgcagataat atatcaattg gtggaattac aaaaacaaat aagaatcaat
      901 ataaagaccc tgtacaaaac gttaatttat tgacttctaa gagtaacggt caaaatactg
      961 cttctgtgac ttcagaatac tatagcatca ataaagaaga aatttcatta aaagaacttg
     1021 atttcaaact aagaaagcaa ttaattgata aacatgatct ttataagaca gagcctaaag
     1081 acagcaaaat taaagtttct atgaaaaatg gcggctacta tacgtttgaa ttaaataaaa
     1141 aattacagcc tcatcgcatg ggtgatacga ttgatagtag aaatataaag aaaattgaag
     1201 tgaatttata ataatattcg agggagtata tcatgagaga aaattttaaa ttacgtaaaa
     1261 tgaaagtcgg tttagtatca gttgcaatta ctatgttata tatcatgaca aatggcgaag
     1321 cagaagcatc tgaaggtagc caaactgtta agaacccaaa ggtgaatgca actgaagaaa
     1381 tcaaagttgg atcacaacca gttcaaaata atcaagaagt aagttcggaa caaacaaaaa
     1441 agaattttgt taatttagac cccattaaac ctggtgctca aaaggtaaca gggactactt
     1501 tacccagcca tattattcta atgaatatag atggtaaaag tgctgattca gtagatggag
     1561 gaaatagtga tttagtattt gctgatgaaa acggaagatt cgagtatcca ctacacaata
     1621 gaaaaattgt tcataatcaa gaaattgagg tttcgtcatc cagtcctgat ttaggtgatg
     1681 atgaagaaga tgaagaagta gaagaagatt caactgaaaa agctggtact gaggaagaaa
     1741 acacagatgc taaagctaca tacacaacac cacgatatga aaaagcgtat gaaataccga
     1801 aagaacaact aaaagaaaaa gatggacatc accaagtttt catcgaacct attactgaag
     1861 gttcaggtat tattaaaggt catacgtctg taaaaggtaa agttgctcta tctattaata
     1921 ataaatttat taattttgaa gaaagagcta aagatggaat tagtaaagaa gatactaaag
     1981 ctagttccga tggtgtttgg atgcctatta atgaaaaagg atattttgat tttgatttca
     2041 aaaaaaatcc ttttgataac ttagagttaa agaaaaatga tgaaatctca ttaacatttg
     2101 cacctgatga tgaagatgaa gcattgaagt cattaatttt caaaactaaa gtaacgagtt
     2161 tagaagatat tgataaagca gaaactaaat atgaccatac taaagtggaa aaagtaaaag
     2221 tattgaaaga tgttaaagaa gatttacatg tagatgaaat ttatggaagt ttgtaccata
     2281 cagaacaagg taaaggtatt ctcgataaac agggaactaa agaaattaca ggtaagacta
     2341 aattcgcgaa tgcagtagtg aaagtatatt ctgacttagg tgatgcgcaa ctgtttcctg
     2401 atattcaagt agatgaaaat ggtaaattta gctttgatgc tgaaaaagct ggtttcagat
     2461 tacaaaatgg agaaacattg aattttgcag tagttaaacc tattactggt gagctattac
     2521 atcaaggatt cgtttctaag tatatcgatg tttatgaatc tccggaagaa aagaaagaac
     2581 gtgaatttga agagaaactt gaaaacacgc ctgcatatca taaattacat ggtgataaaa
     2641 ttgtcggcta tgatgttcaa ggtaatccat caacttggtt ctatccatta ggtgaaaaga
     2701 aagttgaacg taaggcacca aaattagaaa aataattaat aaaataaaac agcttaatga
     2761 tgtaatgaaa ctagtgagtt aatcactgac ttcaacgtca ttgagctgtt ttttgtgctt
     2821 tgttacaaag cattattgag tttattttac gtgttcatat tttgaaacat caaagccgtc
     2881 ttgtttagct ttgttgataa tgtctttgat tgaatgtagt cctttatcgg cgaagtatga
     2941 tcttaagttg tcttttgtag cttggtcagc attcttatct aataacacat cgatatagct
     3001 taattcatgt tctaagaagt ttgcatcatc atgtagtaca agtccatttt gagaataaac
     3061 tttcgcatct gcttgattac tatatccaac aacgccagtt gctaaaacac ctaccattgc
     3121 cgtagctact aaaacctttt taaatttcat atctatcact cctctaaaaa atgtaactct
     3181 atcataacac tgaatattga gaaaattacg tttattaagc cgatttaata attttaataa
     3241 atagttaaag tgacaaatat tgtttaaatg caattaatct ttaatgcgat ggttatggat
     3301 tttcctacta aaaccttgat ttcaaagagg gtttaaatca aatgaaacaa taataaaaaa
     3361 atg
//
LOCUS       DAMZKC010000206         3346 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.209, whole genome shotgun sequence.
ACCESSION   DAMZKC010000206 DAMZKC010000000
VERSION     DAMZKC010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3346
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.209"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..1289
                     /locus_tag="POB55_002418"
     CDS             <1..1289
                     /locus_tag="POB55_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501358.1"
                     /translation="DNAYGEFESLDTINHMNLNEEVLRVRQHTRLNDVTLEHALLLKT
                     NDELKTICRQYGIKGFSNKNKQNLVELIMTAFFNNNHIIEQVFSNATLFEFDILSSIL
                     ESDDNYRLNNELTKFEKLQLINFDNFLTIHFYYYYDFEYDCLILPNDVRKHIENFVNQ
                     VGNGDFEKAVNQFVNEQTIENSLAEFKNALELSFMEDEVDLDMLLEDEDFGTTDSLRS
                     DAITQLISEIKKGLIDKKDILPLRIILGSVNLYGILSTNHLLYLLQRFYDATFSKEEL
                     QYSLDTLAGTELYYVEDDFVFHPVLFDVVESEASGIDISDDGYYVPASVEELIYYDRH
                     HYLKSDQAIKEITSYLRKQINMKNDIEKEKYVNEIIMLLRTIPTSKLIQPIMQLFIDN
                     AILNNVQNDALLIEKATHARNHLRLWSLQGHRDIPN"
     gene            1362..1988
                     /locus_tag="POB55_002419"
     CDS             1362..1988
                     /locus_tag="POB55_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDxxF motif lipoprotein"
                     /protein_id="HDI6501359.1"
                     /translation="MKKRLLLSTFLASALILTGCASDQSDNEDHHTSTGIHAPKSAKK
                     LETKDIFNSDKKNSDISDAEMKQAIEKYLSVNSDILENKYIMQHKLDKQIDSQTKVTE
                     KQAETLSHLSNLAVKNDLHFKKFVTENNIPKEYKKPVELMMNYFKALNSTIANVDEDI
                     EKLSYQPQNKINVVDVPTKYAGDVNKKQQDKIKDFLKSKGIKSDVIDK"
     gene            2243..2626
                     /locus_tag="POB55_002420"
     CDS             2243..2626
                     /locus_tag="POB55_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501360.1"
                     /translation="MKVRITFIILAILSTIVCLFLTAMHPTGPNTVTFEQPYLFTLNI
                     IIMVLVALPSLVLAIYDYMSFRISSAILQFLGAISWFFLSLILSLTQFTPFTLASFIT
                     SIILFVSAIITLAIGGKSVDKNDFH"
     gene            complement(2692..3273)
                     /locus_tag="POB55_002421"
     CDS             complement(2692..3273)
                     /locus_tag="POB55_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDI6501361.1"
                     /translation="MTIYLVRHGESKSNYDNKHFRSYFCGQLDVPLTDTGTKSADDLC
                     DYFKEKQIKHVYVSDLLRTQQTFEHIFPYDIASTTTPLLRERSLGVFEGEYKDEISAN
                     PKYEKYFNDPNFKDFRHSFSQKAPEGESYEDVYQRVEHFMNHVVNEDTQKDDIVIVAH
                     QVVIRCLMVYFNKVSREEAVDLKVENCKPYIIE"
BASE COUNT         1189 a          537 c          488 g         1132 t
ORIGIN      
        1 tagataatgc atatggtgaa ttcgaatcgt tagatacaat aaaccatatg aatttaaatg
       61 aagaagtgtt acgtgtaaga caacatacgc gcttaaacga tgtcacatta gaacatgctt
      121 tactgttaaa aactaatgac gagctaaaaa caatatgcag gcagtatggt atcaaaggat
      181 tctcaaataa aaacaaacaa aatcttgttg aattaattat gacagcattt ttcaataata
      241 atcacatcat tgaacaagtg ttttctaatg ctactctatt tgagtttgat attctttcta
      301 gcatacttga aagtgatgat aactaccgat taaacaatga actaactaaa tttgaaaagc
      361 tgcaattaat aaattttgat aactttttaa ctattcattt ttattattat tacgactttg
      421 aatatgattg tcttattttg ccaaatgatg tgcgtaaaca tattgaaaat ttcgtgaatc
      481 aagtcggcaa tggtgatttt gagaaagctg taaatcaatt tgttaatgag caaacaattg
      541 agaatagttt agcagagttt aaaaacgcgt tagaattatc atttatggaa gacgaagttg
      601 acttagatat gttacttgaa gatgaagatt ttggtacaac cgatagctta agaagtgatg
      661 caatcactca attgatttct gagattaaga aaggattaat agataaaaaa gatattttac
      721 ctcttcgtat tatacttggt tctgtaaact tgtatggtat tttatcaact aatcatctac
      781 tatatctttt acaaagattt tatgacgcta cttttagtaa agaagagtta caatatagct
      841 tagatacttt agctggtact gagctatatt atgttgaaga tgacttcgtg tttcatcctg
      901 tgttatttga tgttgtcgag tctgaggcgt ctggcattga tatttctgat gatggatatt
      961 atgtaccagc ttcggttgaa gaattaatct attatgatag acatcactat ttgaaatctg
     1021 atcaagcaat taaagagatc accagttatc tacgcaaaca aatcaatatg aaaaatgata
     1081 ttgagaagga aaagtatgtg aacgaaatca ttatgctttt acgtacgata cctacgtcta
     1141 aattgataca accaattatg cagttattta tagataatgc tatccttaac aacgttcaaa
     1201 acgacgcatt attgattgaa aaagcaactc atgcacgtaa tcatttacgt ctatggtcat
     1261 tgcaaggtca tcgtgacatt ccaaactaat tcaatgttac tctattaaac atgaatacac
     1321 attttttcaa ttaaagataa actaaggagt tatattacat tatgaaaaag agattactac
     1381 taagtacatt tttagcatct gcattaattc ttacaggttg cgcgtccgat caatctgata
     1441 acgaagatca tcacacaagt actggcatcc atgcacctaa aagcgctaaa aaattagaaa
     1501 caaaagatat ctttaactca gataagaaaa atagtgatat tagtgatgca gagatgaagc
     1561 aagctataga aaagtatttg tctgtaaata gcgatatact tgagaacaaa tatattatgc
     1621 aacataaact agataaacaa attgacagtc aaacaaaagt gactgaaaaa caagcagaaa
     1681 cacttagcca cttatctaac ttagctgtta aaaacgattt gcatttcaag aaatttgtca
     1741 ctgaaaacaa tattcctaaa gaatataaga aacccgttga attaatgatg aattacttca
     1801 aagctttaaa tagtacaatc gccaatgttg atgaagatat tgaaaagctt agctatcaac
     1861 cgcaaaataa aatcaatgta gtcgacgtgc caacaaaata tgccggtgat gttaataaaa
     1921 agcaacaaga taaaattaaa gatttcttaa aatctaaagg tatcaagagt gatgttatcg
     1981 ataaataact gaaagccatc attgcgacat gtaacacatc attacatgag ctttaattca
     2041 tgacaattct ttatctttaa aagtgactaa tcaatttagc taaagtcatc atttgggcat
     2101 caagcagcat attactgttt gatgcttttt taattgcctt aaattcacta tccatcctaa
     2161 aattcccatt agttgacgca tttgactata tacctaacat attccatact tatattgact
     2221 ctataaacaa aggatgatca tgatgaaagt tcgaattact tttattattt tagcaatttt
     2281 atcgacgatt gtttgcttgt tcttaacagc aatgcatcca acaggaccaa acacagtaac
     2341 ttttgaacag ccatatttat tcaccctcaa catcattata atggtattag tcgcattacc
     2401 ttcacttgtt ttagctatat atgattatat gagttttaga atttcttctg ctattttaca
     2461 atttctaggg gctatctctt ggttcttttt atcattgata ttatcgctca cacaatttac
     2521 accttttaca ttagcgtcat ttataacttc aattattttg ttcgtaagcg caattatcac
     2581 attagctatt ggtggtaagt ctgttgataa gaatgacttc cattaaattc caaatacaaa
     2641 aaaggttctg aaggcagcta taaaacacag cttttcagaa cctctatact tctattcaat
     2701 gatatatggt ttgcaatttt caacttttaa atccacagct tcttcccttg aaactttgtt
     2761 aaaataaacc atcaaacaac gaatgacaac ttgatgtgca acaatgacga tatcatcttt
     2821 ttgtgtatct tcattgacaa catgattcat aaaatgttcg acgcgttgat atacatcttc
     2881 ataactttct ccttcaggcg ctttttgtga aaaactatga cgaaagtctt taaagtttgg
     2941 atcattgaaa tatttttcat atttcggatt cgcactgatt tcatctttat attcaccctc
     3001 aaatacgcca agtgaacgtt ctcttaatag aggcgtagtc gttgatgcaa tgtcatatgg
     3061 aaaaatatgt tcaaacgttt gctgtgttct taataagtct gaaacatata catgtttaat
     3121 ctgtttctct ttaaaataat cacataaatc gtctgcactt tttgtgccag tatccgttaa
     3181 cggcacatct aattgtccac aaaaataaga tcgaaaatgt ttattatcat aattcgattt
     3241 tgattcgcca tgtctaacta aataaatcgt cataatatta ctccttacct tatgtatttc
     3301 atatctacca taacactttg actactaatt cgatatcaat cttaat
//
LOCUS       DAMZKC010000207         3314 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.210, whole genome shotgun sequence.
ACCESSION   DAMZKC010000207 DAMZKC010000000
VERSION     DAMZKC010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3314
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.210"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            24..782
                     /locus_tag="POB55_002422"
     CDS             24..782
                     /locus_tag="POB55_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDI6501362.1"
                     /translation="MYISILILIVFIAGCGKSDKTKEDSKEAQIKKSFEKTLDMYPIK
                     NLEDLYDKEGYRDGEFKKGDKGTWTLLTSFAKSNKPGEIDDEGMVLFLNRNIKKATGY
                     YYTSKVHDEFNEKEHQKKYHVELKNNKIVLLDNVEDSKLKNKIENFKFFSQYADFRDF
                     KNYKNGNISSADNVPSFDAEYQISNTDKNVKKLREVYPITTKKSPVLKLHIDGDIKGS
                     SIGYKNIEFNFSKVKDEETAVRDFVNFGPSDGVS"
     gene            830..1600
                     /gene="lpl5"
                     /locus_tag="POB55_002423"
     CDS             830..1600
                     /gene="lpl5"
                     /locus_tag="POB55_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl5"
                     /protein_id="HDI6501363.1"
                     /translation="MGYLKRIGMCISLLIVIIFVTSCGGGNKITGDSKETQIKKSFAK
                     TLDMYPIKNLEDLYDKEGYRDGEFKKGDKGMWTIYTDFAKSNKPGELDDEGMVLNLDR
                     NTRTAKGYYFVKKFYEKDKLPDRKNYKVEMKNNKSILLDKVEDPNLKKRIENFKFFGQ
                     YANFKDLENYNNGDVSINWNVPSYDVEYKMSNKDENVKQLRSRYNIPTDKAPMLKMHI
                     DGDLKGSSVGYKRLEIDFSKEDRDISVIDYLSYKPAKK"
     gene            1632..2423
                     /locus_tag="POB55_002424"
     CDS             1632..2423
                     /locus_tag="POB55_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000456136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDI6501364.1"
                     /translation="MEYLKRLALFISVIILTIFIMGCDSQSDTAENPKEGSKEAQIKK
                     SFSKTLDMYPIKNLEDLYDKEGYRDGEFKKGDKGTWTISTDFAKSNKPGEMDSEGMVL
                     HLNRNMRTATGHYTIRTTYDELGKMTREKNYRVELKNNKIVVLDKVEDVNLKHKIENF
                     KFFSQYADFKDLKNYKNGRISINENVPYYEAEYKRNNSDGNVKKLREKYPITTKQSPI
                     LKLHIDGDIKGSSVGYKQIEYMFSKEKEDETFMSDFLNFGPSHSN"
     gene            2475..3273
                     /locus_tag="POB55_002425"
                     /pseudo
     CDS             2475..3273
                     /locus_tag="POB55_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649608.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
BASE COUNT         1417 a          338 c          633 g          926 t
ORIGIN      
        1 tgggatattt aaaagggttt gcattgtaca taagtatctt aattttaata gtttttatag
       61 caggttgtgg caaaagtgat aaaacaaaag aagattcaaa agaagcacaa attaaaaaga
      121 gctttgagaa aactttagat atgtatccaa tcaaaaatct cgaggattta tacgacaaag
      181 aaggctatag agatggtgaa tttaaaaagg gtgataaggg tacatggact ttactcacaa
      241 gttttgctaa aagtaacaaa ccaggtgaga tagatgatga aggcatggtt ttatttttaa
      301 atagaaatat aaagaaggca acaggatatt attacacgag caaagttcat gatgaattta
      361 atgaaaaaga gcatcaaaaa aaatatcatg ttgaacttaa aaataataaa atagttcttt
      421 tggataatgt agaagattca aagctcaaaa ataaaataga aaattttaaa ttctttagtc
      481 agtacgctga ttttagagat tttaaaaact ataaaaatgg aaatatatca agtgcagata
      541 atgtaccaag tttcgatgca gaatatcaaa taagtaatac ggataaaaat gtaaaaaaac
      601 ttagagaggt ttatccaatc acaacaaaaa aatctccagt attaaaatta catatagatg
      661 gtgacataaa aggaagttca attggatata aaaatataga atttaatttt tcaaaagtaa
      721 aagacgaaga aacagctgtt agagattttg tgaattttgg accatctgat ggagttagct
      781 aaagataatt agatgtagaa ataattctaa aacagagaga cgtgaaacga tgggatattt
      841 aaaaaggatt ggaatgtgta taagcctgtt gattgtaatc atttttgtaa catcttgcgg
      901 tggtggtaat aaaatcactg gagattcaaa agaaacacaa atcaaaaaga gttttgcgaa
      961 aacgttagat atgtacccaa tcaaaaatct tgaagattta tacgacaaag aaggatatcg
     1021 agatggcgaa tttaaaaaag gtgacaaagg gatgtggacg atatacacag atttcgctaa
     1081 aagtaataaa ccgggtgaat tggatgatga aggtatggtt ttaaatctgg atagaaatac
     1141 tcgaacggct aagggatatt attttgttaa gaaattttat gaaaaggata aattacctga
     1201 tagaaaaaat tataaagttg aaatgaaaaa taataaaagt atcttattag acaaggtaga
     1261 agatccaaat ctaaaaaaga gaatagaaaa ctttaaattt ttcggacaat atgcaaattt
     1321 taaggatttg gaaaattaca acaatggcga cgtgtcaata aattggaatg ttccaagtta
     1381 tgacgtggaa tataaaatga gcaataaaga tgaaaatgtt aaacaattaa gaagtcgtta
     1441 taacattcct actgataaag ctccaatgtt aaaaatgcat attgacgggg acttaaaagg
     1501 tagttctgtt ggatataaaa ggttagaaat agatttttca aaagaagata gggatatttc
     1561 agtcattgat tatttaagtt ataagccagc gaaaaaatag tactgataat taaatattag
     1621 ggtgtgaagg aatggaatat ctaaaaaggc ttgcattgtt cataagtgtt attattttga
     1681 ccatttttat aatgggttgt gatagtcaaa gcgatactgc agaaaatcca aaagaaggtt
     1741 caaaagaagc acaaattaaa aagagttttt cgaaaacgtt agatatgtat ccaattaaga
     1801 atctcgagga tttatatgac aaagaaggat atcgtgatgg cgaatttaaa aaaggtgata
     1861 aagggacgtg gacaatatca acagattttg ctaaaagcaa caaaccaggt gaaatggata
     1921 gtgaaggtat ggtgctacat ttgaatagaa atatgaggac ggcaactggg cattatacta
     1981 taagaacaac ttatgatgaa ttgggtaaga tgacacgcga aaaaaattat cgtgttgaac
     2041 ttaaaaataa taagatagta gttttagata aagtagaaga tgtaaacctt aaacataaaa
     2101 ttgaaaattt taaatttttt agtcagtatg cagattttaa agatttgaaa aattataaaa
     2161 atggaagaat atctatcaat gaaaatgttc cgtattatga agcagagtac aaaaggaata
     2221 atagtgatgg aaatgtaaaa aaacttagag aaaagtaccc aattacaacc aagcagtctc
     2281 caatattaaa actgcatata gacggagata ttaaaggtag ctcagttgga tataagcaga
     2341 tagagtacat gttttctaag gagaaagagg atgagacttt tatgagtgat tttttgaact
     2401 tcggaccatc acatagtaat tagtaaataa aaccaggggc ataaactaat ttaaaacaag
     2461 agagaagtga aaagatggga tattttaaaa gagttttact ttatataatt gttatggttt
     2521 tgagtgtttt tataataggt tgtgataaat caagcgatac ttcagaaaaa tcaaaaggag
     2581 attcaaaaga agcacaaatt aaaaagagtt ttgcaaaaac gttagacatg tatcctactg
     2641 aaaatctaga agacttttat gacaaagagg gatatcgaga tggaaaattt aaaaaaggcg
     2701 acaaaggtac ttgggttatt agatctgaaa tgacaacaga actgaaaaat gaaaatatgg
     2761 tatctaaagg tatggtcata cgtttaaata gaaatagtag aacatgcact ggtgaatatt
     2821 ttgtcaggat agttaaagaa gacagtgagg gcaaggtata tagtgatgaa cgaaaatatc
     2881 cagtgaaaat ggaaaataat aaaatcattc cattaaaacc aatcgatgat gaaaaagtaa
     2941 aaaaaagaaa ttgaagaatt taaattcttt gtacaatacg ggaatttcaa agaattggaa
     3001 aactataaag acggagaagt gacatataac ccagaagcac caatatactc tgcacaatat
     3061 caattgaaaa acagtgatta caatgtagaa caattacgta agcgatataa tatcccgacg
     3121 caaaaagcgc ctaaattatt attgaaaggc tcaggtaatt taaaaggttc atcagtcgga
     3181 tataaaaata ttgaatttac ctttgttgaa aacaaggaag aaaatattta cttcacagat
     3241 agtgtctact ttaatccaag cgaggataaa taagtataat tattattata aaaaatgacg
     3301 ttagaacatt taat
//
LOCUS       DAMZKC010000208         3211 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.211, whole genome shotgun sequence.
ACCESSION   DAMZKC010000208 DAMZKC010000000
VERSION     DAMZKC010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.211"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            42..275
                     /locus_tag="POB55_002426"
     CDS             42..275
                     /locus_tag="POB55_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HDI6501365.1"
                     /translation="MDYIGQYAVIHLVLHVVCICIAYWALQSIRLDQFFKKGYATQLQ
                     VCMIFVAILLGTAVSNFIVDLLQYSTQVKYLIK"
     gene            386..1651
                     /gene="murA"
                     /locus_tag="POB55_002427"
     CDS             386..1651
                     /gene="murA"
                     /locus_tag="POB55_002427"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDI6501366.1"
                     /translation="MDKIVIKGGNKLTGEVKVEGAKNAVLPILTASLLASDKPSKLVN
                     VPALSDVETINNVLTTLNADVTYKKDENAVVVDATKTLNEEAPYEYVSKMRASILVMG
                     PLLARLGHAIVALPGGCAIGSRPIEQHIKGFEALGAEIHLENGNIYANAKDGLKGTSI
                     HLDFPSVGATQNIIMAASLAKGKTLIENAAKEPEIVDLANYINEMGGRITGAGTDTIT
                     INGVESLHGVEHAIIPDRIEAGTLLIAGAITRGDIFVRGAIKEHMASLVYKLEEMGVE
                     LDYQEDGIRVRAEGELQPVDIKTLPHPGFPTDMQSQMMALLLTANGHKVVTETVFENR
                     FMHVAEFKRMNANINVEGRSAKLEGKSQLQGAQVKATDLRAAAALILAGLVADGKTSV
                     TELTHLDRGYVDLHGKLKQLGADIERIND"
     gene            1685..2125
                     /gene="fabZ"
                     /locus_tag="POB55_002428"
     CDS             1685..2125
                     /gene="fabZ"
                     /locus_tag="POB55_002428"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDI6501367.1"
                     /translation="METIFDYNQIKQIIPHRQPFLLIDKVVEYEEGQRCVAIKQVSGN
                     EPFFQGHFPEYAVMPGVLITEALAQTGAVAILNSEENKGKIALFAGIDKCRFKRQVVP
                     GDTLTLEVEITKIKGPIGKGNAKATVDGQLACSCELTFAIQDVK"
     gene            complement(2183..2623)
                     /locus_tag="POB55_002429"
     CDS             complement(2183..2623)
                     /locus_tag="POB55_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwpF-like family protein"
                     /protein_id="HDI6501368.1"
                     /translation="MKTFKAVRFQIVNEHGRIIEYELEDGVIINKEESGTGWLLEIVI
                     SNEHYETFKEYQDNEQLLDIRVVITRPANDPALFESTVKSIKNFKTTMSIVFECHIYT
                     LRQQYAESLLEQLIDDGLSGEELKKSFNRMMQSKPKLKDEKLEE"
     gene            2817..>3211
                     /locus_tag="POB55_002430"
     CDS             2817..>3211
                     /locus_tag="POB55_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDI6501369.1"
                     /translation="MLNKIVIVERLTKDAQIFEKEDRKIATFCVATHRNYKDENGEIV
                     CDYLFCKAFGKLASNIEKYTNQGTLVGITGQMRSRKYDKDGQTHFVTELYVETIKFMS
                     PKSQNNEILSDSILDIDSQNIDNHDLLEI"
BASE COUNT         1145 a          455 c          581 g         1030 t
ORIGIN      
        1 ttcatgtaca atgtaataca gtaatcttat gaggtgataa aatggattat atcggacaat
       61 atgcagttat ccatttagtg ttacatgttg tatgtatttg tattgcctat tgggctttac
      121 aatcaattag attagatcaa ttttttaaaa aaggatacgc cactcaatta caagtgtgta
      181 tgatatttgt tgctatttta ttaggcactg cagtaagcaa ttttattgta gatttgttac
      241 aatactcgac gcaggtaaaa tatttaataa aataagtcta actctatgat ttgtaatcaa
      301 aactagatat aattaaataa tgacttaaaa taattttaaa atagggaaat gtaaagtaat
      361 aggagttcta agtggaggat ttacgatgga taaaatagta atcaaaggtg gaaataaatt
      421 aacgggtgaa gttaaagtag aaggtgctaa aaatgcagta ttaccaatat tgacagcatc
      481 tttattagct tctgataaac cgagcaaatt agttaatgtt ccagctttaa gtgatgtaga
      541 aacaataaat aatgtattaa caactttaaa tgctgacgtt acatacaaaa aggacgaaaa
      601 tgctgttgtc gttgatgcaa caaagactct aaatgaagag gcaccatatg aatatgttag
      661 taaaatgcgt gcaagtattt tagttatggg acctctttta gcaagactag gacatgctat
      721 tgttgcattg cctggtggtt gtgcaattgg aagtagaccg attgagcaac acattaaagg
      781 ttttgaagct ttaggcgcag aaattcatct tgaaaatggt aatatttatg ctaatgctaa
      841 agatggatta aaaggtacat caattcattt agattttcca agtgtaggag caacacaaaa
      901 tattattatg gcagcatcat tagctaaggg taagacttta attgaaaatg cagctaaaga
      961 acctgaaatt gtcgatttag caaactacat taatgaaatg ggtggtagaa ttactggtgc
     1021 tggtacagac acaattacaa tcaatggtgt agaatcatta catggtgtag aacatgctat
     1081 cattccagat agaattgaag caggcacatt actaatcgct ggtgctataa cgcgtggtga
     1141 tatttttgta cgtggtgcaa tcaaagaaca tatggcgagt ttagtctata aactagaaga
     1201 aatgggcgtt gaattggact atcaagaaga tggtattcgt gtacgtgctg aaggggaatt
     1261 acaacctgta gacatcaaaa ctctaccaca tcctggattc ccgactgata tgcaatcaca
     1321 aatgatggca ttgttattaa cggcaaatgg tcataaagtc gtaaccgaaa cagtttttga
     1381 aaaccgtttt atgcatgttg cagagttcaa acgtatgaat gctaatatca atgtagaagg
     1441 tcgtagtgct aaacttgaag gtaaaagtca attgcaaggt gcacaagtta aagcgactga
     1501 tttaagagca gcagccgcct taattttagc tggattagtt gctgatggta aaacaagcgt
     1561 tactgaatta acgcacctag atagaggcta tgttgactta cacggtaaat tgaagcaatt
     1621 aggtgcagac attgaacgta ttaacgatta attcagtaaa ttaatataat ggaggatttc
     1681 aaccatggaa acaatttttg attataacca aattaaacaa attatacctc acagacagcc
     1741 atttttatta attgataaag tagttgaata tgaagaaggt caacgttgtg tggctattaa
     1801 acaagtatca ggaaacgaac cattctttca agggcatttt cctgagtatg cggtaatgcc
     1861 aggcgtatta attactgaag cgttagctca aacaggtgcg gtagctattt taaatagtga
     1921 agaaaataaa ggtaaaatcg ctttatttgc tggtattgat aaatgtcgtt ttaaacgtca
     1981 agtagtacct ggtgatactt taacgttgga agtagaaatc actaaaatta aaggaccaat
     2041 cggtaaaggt aatgctaaag ctactgtcga tggtcaactt gcttgtagtt gtgaacttac
     2101 atttgcaatt caagatgtaa aataaaacaa aaaaacattc aaagatttaa tgtgttggca
     2161 aaatctttga atgtttttta ttttactctt ctaatttttc atcctttaac tttggtttag
     2221 actgcatcat tcgattaaat gattttttta attcttcacc agataatcca tcatcaataa
     2281 gttgttctaa taaactttca gcatactgtt gacgtaaagt gtaaatatga cattcaaata
     2341 cgatagacat tgttgtttta aaatttttaa tacttttcac agtagattca aataaagctg
     2401 gatcattggc tggacgcgtg atgacgacac gaatatccaa taactgttca ttgtcttgat
     2461 attccttaaa tgtttcataa tgctcattag aaatgacaat ttcaagcaac caacccgtgc
     2521 cactttcctc tttattaatt attacgccat cttctaattc atattctata attctgccgt
     2581 gttcattcac aatttgaaac cttactgctt taaaagtttt catattccca catcccatta
     2641 gtttaatatt tatgattttt gaattattta gaagatttaa ggtcatttca tgactaattt
     2701 ctttaaatat aacaaataac gagatgctat agctattaat tatataaaat ttgccatttt
     2761 gaccattttt aagctagtaa ttaagatata ggcataggag gtgaatcaca cactacatgc
     2821 taaataaaat cgtaattgtc gagagactga cgaaagacgc acaaatattt gaaaaggagg
     2881 atagaaaaat tgcaacgttt tgtgttgcaa cgcaccgaaa ttataaagat gaaaatggag
     2941 aaatcgtctg tgattactta ttctgtaaag catttggcaa gttagcttct aatatagaaa
     3001 aatatactaa tcaaggtaca ttggttggta taactggtca aatgagatca agaaagtatg
     3061 ataaagacgg acaaacacac tttgtcactg aattatatgt tgaaacaata aaatttatgt
     3121 cccctaaatc ccaaaataat gaaattctct cagatagtat tttagatatt gactctcaaa
     3181 atatagataa tcatgactta ttagaaattt a
//
LOCUS       DAMZKC010000209         3196 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.212, whole genome shotgun sequence.
ACCESSION   DAMZKC010000209 DAMZKC010000000
VERSION     DAMZKC010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.212"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            49..1008
                     /locus_tag="POB55_002431"
     CDS             49..1008
                     /locus_tag="POB55_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDI6501370.1"
                     /translation="MNRNIVKLVVFMLILVVAVAGCGQKDTEEKTEMTTIKDELGTEK
                     IKKNPKRVVVLEYSFADYLAALDMKPVGIADDGSSKNITKSVRDKIGAYESVGSRPQP
                     NMEVISKLKPDLIIADVSRHKKIKSELSKIAPTIMLVSGTGDYNANIEAFKTVAKAVG
                     KEKEGEKRLEKHDKILAEIRKKIEQSTLKSAFAFGISRAGMFINNEDTFMGQFLLKMG
                     IQPEVTKDKTTHVGERKGGPYIYLNNEELANINPKVMILATDGKTDKNRTKFIDPAVW
                     KSLKAVKDNKVYDVDRNKWLKSRGIIASESMAEDLEKIAEKAK"
     gene            complement(1054..1185)
                     /locus_tag="POB55_002432"
     CDS             complement(1054..1185)
                     /locus_tag="POB55_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501371.1"
                     /translation="MRYVTMRQFIKRIVKTILVGYVIKFIRNKLSGKSSHPTDNKHN"
     gene            complement(1249..1464)
                     /locus_tag="POB55_002433"
     CDS             complement(1249..1464)
                     /locus_tag="POB55_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HDI6501372.1"
                     /translation="MKVNKAIYVIVALFLGGLGIHKFYADKFGQGLFHLLFFWTGIPT
                     IISIIHAIFVIFTKKADKDGYIIFPKN"
     gene            1629..2180
                     /locus_tag="POB55_002434"
     CDS             1629..2180
                     /locus_tag="POB55_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6501373.1"
                     /translation="MIRQARPEDRFDIAKLVYMVWDDMELELVKHLPKDMVLDAIEKS
                     CVDATYRTFYQHILVYEVESKVAGCIISYSGENELKYEKAWELLDLPEEIKQYGTPLP
                     VKEAKDDEYYIETIATFEAYRGRGIATKLLTSLLESSTHVKWSLNCDINNEAALKLYK
                     KVGFISDGQIELYKHMYHHLIVK"
     gene            complement(2231..3169)
                     /locus_tag="POB55_002435"
     CDS             complement(2231..3169)
                     /locus_tag="POB55_002435"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HDI6501374.1"
                     /translation="MSNQYQQYSTVKKYWHLMRPHTLTASVVPVLVGTAASKIYFLGS
                     EDHIKISLFIAMLLACLLIQAATNMFNEYYDYKKGLDDHESVGIGGAIVRNGMSPELV
                     LRLAIAFYILAAILGLFLAANSSFWLLPVGLVCMAVGYLYTGGPFPISWTPFGELFSG
                     VFMGMFIIVIAFFIQTGNIQSYVIWLSVPIVITIGLINMANNIRDRVKDKASGRKTLP
                     ILLGKNASLTFMAIMYFIAYAFIVLTIIIKPGGSLFYLLALLSFPMPVKVIRRFKKND
                     TPPTMMPAMAAAGKTNTFFGLLYALGIYISALFAGI"
BASE COUNT         1211 a          448 c          586 g          951 t
ORIGIN      
        1 ataaaataat aatatagttt tctactaaag ttgtttggag gtatatgtgt gaataggaat
       61 atcgttaaac tagttgtgtt tatgctaatc ttagttgtag cagtagcggg ttgtggtcaa
      121 aaagatactg aagagaaaac tgaaatgacg acaataaaag atgaattagg aactgaaaaa
      181 attaagaaaa atcctaaacg tgttgttgta ttagaatata gttttgctga ttatttagca
      241 gcattagata tgaaacctgt tggtattgca gatgatggca gcagtaaaaa tataacaaag
      301 tcagtaagag ataagattgg ggcatatgaa tcggttggat ctagaccgca accgaatatg
      361 gaagtgataa gtaaattaaa accggatttg atcattgcag atgttagcag acataagaaa
      421 atcaaatcag aattgagcaa aattgcgccg acaattatgt tagtcagtgg tacgggagat
      481 tacaatgcaa atattgaagc atttaaaaca gtcgctaaag cagttggcaa agagaaagaa
      541 ggcgagaagc gtctggaaaa gcatgataaa atattagcgg agattagaaa gaaaattgaa
      601 cagagtacgt taaaatctgc atttgcattc ggtatctcaa gagcaggtat gtttattaat
      661 aatgaagata catttatggg acaattctta cttaaaatgg gtattcaacc tgaagtcaca
      721 aaagacaaaa ctacgcatgt tggtgaacgc aagggtggtc cttatatata tttaaataat
      781 gaagaacttg ccaatatcaa tccaaaagtt atgattttag ccactgacgg gaaaacggac
      841 aaaaatagaa cgaaattcat tgatcctgca gtttggaaat cattaaaagc tgtgaaagat
      901 aacaaagttt atgacgttga ccgaaataag tggttgaaat caagggggat tatcgcaagt
      961 gaaagtatgg cagaagattt agaaaaaatt gcagaaaaag caaaataaaa atacagcgct
     1021 actcgtaaat catataagag tggcgctgta tttttaatta tgtttattat ctgtcggatg
     1081 tgatgattta cctgaaagtt tatttcgaat aaatttaatt acataaccga caaggattgt
     1141 tttaacaatt cttttaatga attggcgcat cgttacatac ctcatttctc tatatcttat
     1201 gaactatata cccattcata tatgcttttt aaacgtcatt gtcactattt aatttttagg
     1261 gaatataata taaccatctt tatctgcttt tttagtaaaa atgacaaaaa tggcatgtat
     1321 tattgagatg atggtaggga tacctgtcca gaaaaataat aagtgaaaaa gaccttgtcc
     1381 aaatttatca gcataaaatt tatgaatacc taaacctcca agaaataatg caacaataac
     1441 ataaatggct ttattgactt tcatttgtaa atcctcctta actataattc tacttaaatt
     1501 cgttgtgaaa accaatattt ttaactttag aattttcaaa ctttctaaaa ttataagtat
     1561 atctttttaa aataagctag aatttctata taataaatgt taataacgta aaagggaatg
     1621 atgacatagt gatacgtcaa gcacgtccag aggaccgatt tgatattgcg aagttagttt
     1681 atatggtgtg ggatgatatg gaattagaat tggtaaagca tctacctaaa gacatggtat
     1741 tagatgcaat tgaaaaaagc tgtgttgatg caacatatcg aactttttat cagcatattt
     1801 tagtttatga agtagaaagt aaagtagcag gttgtattat tagctatagc ggtgaaaatg
     1861 aattgaaata tgaaaaagca tgggaactac ttgacttgcc agaagaaata aaacaatatg
     1921 gcacgccatt acctgtaaaa gaagctaaag acgatgagta ttatatagaa acaattgcga
     1981 catttgaagc atatagaggt agaggcatcg cgacaaagtt attaacgtca ttacttgaat
     2041 caagtacaca tgttaaatgg agtttgaatt gcgatattaa taatgaagca gcattaaagt
     2101 tatataaaaa agtaggcttt atatctgatg gacagattga attatacaag cacatgtatc
     2161 atcatttaat tgttaaataa aaaactcgac agttcgatgt aagtcgattg ccgagtagta
     2221 tcattttata ttaaatgcct gcaaataatg cactaatata aatacctaat gcatataata
     2281 aaccgaaaaa tgtatttgtt ttaccagcag cagccattgc tggcatcatt gtaggcggtg
     2341 tatcattctt cttgaaacgt ctgataactt taacaggcat tgggaatgat aacaacgcaa
     2401 gtaagtaaaa taatgagcca ccaggtttaa taatgatcgt aagtacaata aaggcataag
     2461 cgataaagta catgattgcc ataaatgtta aagaagcatt tttacctaat agaatgggta
     2521 aagttttgcg accacttgct ttatctttga cacggtcgcg aatattgtta gccatattaa
     2581 ttaaaccgat agtgattact ataggtacac ttaaccaaat tacataactt tgaatattgc
     2641 cagtttgaat aaagaatgca ataacgataa taaacatacc cataaatacg cctgagaata
     2701 attcaccgaa aggcgtccat gaaataggga aaggaccacc tgtatatagg taaccaacag
     2761 ccatacatac taatccaact ggtaataacc aaaatgaaga gttagcagct aaaaacaaac
     2821 ctaatattgc tgctaagatg taaaatgcaa tggctaatcg tagcacaagc tctgggctca
     2881 taccgttgcg aacaatggca ccaccaatgc ctacagattc atgatcatca aggccttttt
     2941 tataatcata gtattcatta aacatattag ttgctgcttg aataagtaag catgctagta
     3001 acatggcaat gaataggctg attttaatat gatcttcgct accaagaaaa tatattttag
     3061 atgctgctgt accaactaaa acgggtacta cggaagcagt taatgtatga ggacgcatta
     3121 aatgccaata tttcttaact gtagaatatt gctgatattg attactcatt gaatacaaaa
     3181 cctctttaaa tcatat
//
LOCUS       DAMZKC010000210         3107 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.213, whole genome shotgun sequence.
ACCESSION   DAMZKC010000210 DAMZKC010000000
VERSION     DAMZKC010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3107
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.213"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(35..739)
                     /locus_tag="POB55_002436"
     CDS             complement(35..739)
                     /locus_tag="POB55_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HDI6501375.1"
                     /translation="MYNEILGLVTFIATFVLMVLMYRFFGKQGLIAWVAIGTIIANIQ
                     VIKTVEIFGISATLGNVMFASIYLATDILNDIYGRRVAKRAVWLGFSSTLIMIIVMQL
                     SLHFIPAPEDMAQKALHAIFDVVPRIALGSIVAYIIGQHIDVFIFSLIKKVFSSDKTF
                     FIRAYGSTFLSSIIDTALFVAIAFIGSLPGTVVFEIFITTYVLKLASTVFNVPFGYIA
                     KSFYRKGKIDKLDQGY"
     gene            984..1178
                     /locus_tag="POB55_002437"
     CDS             984..1178
                     /locus_tag="POB55_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HDI6501376.1"
                     /translation="MSRILTFSEQNALAKIDDLMNTYCNQCPIKTRLRKLEGKTKAHH
                     FCINECSIGKEIKQLGNELQ"
     gene            1190..1441
                     /locus_tag="POB55_002438"
     CDS             1190..1441
                     /locus_tag="POB55_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU N-terminal domain-containing protein"
                     /protein_id="HDI6501377.1"
                     /translation="MKIISISETPNHNTMKITLSESREGMTSDTYTKVDDSQPAFIND
                     ILKVEGVKSIFHVMDFISVDKENDANWETVLPKVEAVFE"
     gene            1480..2604
                     /locus_tag="POB55_002439"
     CDS             1480..2604
                     /locus_tag="POB55_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor C"
                     /protein_id="HDI6501378.1"
                     /translation="MEILRIEPTPSPNTMKVVLSYTREDKLSNTYKKVEETQPRFINQ
                     LLSIDGITSIFHVMNFLAVDKAPKADWEVILPDIKAAFSDANKVLESVNEPQIDNHFG
                     EIKAELLTFKGIPYQIKLTSADQELREQLPQTYVDHMTQAQTAHDNIVFMRKWLDLGN
                     RYGNIEEVMDGVLEEVLATYPESQLPVLVKHALEENHATNNYHFYRHVSLDEYHATDN
                     WKTRLRMLNHFPKPTFEDIPLLDLALSDEKVPVRRQAIVLLGMIESKEILPYLYKGLR
                     DKSPAVRRTAGDCISDLGYPEALPEMVLLLDDPQKIVRWRAAMFIFDEGNAEQLPALK
                     AHINDNAFEVKLQIEMAISRIENGDEALGSVWKQMANRTI"
     gene            2620..3057
                     /locus_tag="POB55_002440"
     CDS             2620..3057
                     /locus_tag="POB55_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HDI6501379.1"
                     /translation="MNAYDAYMKEIAQQMRGELTQNGFTSLETSEAVSEYMNQVNADD
                     TTFVVINSTCGCAAGLARPAAVAVATQNEHRPTNTVTVFAGQDKEATATMREFIQQAP
                     SSPSYALFKGQDLVYFMPREFIEGRDINDIAMDLKDAFDENCK"
BASE COUNT         1151 a          475 c          548 g          933 t
ORIGIN      
        1 gccttaaaac ttattctttt gtgtagtaag tgcgttaata gccttgatct aacttatcaa
       61 tcttaccttt acgataaaat gatttagcaa tatatccaaa tggtacattg aaaactgttg
      121 aagctaattt taatacgtac gttgtaataa atatttcaaa tacaactgta ccaggtaaac
      181 ttccgataaa tgcgatagct acaaataaag ctgtatcaat tattgagctt aaaaatgtac
      241 taccatatgc acggatgaaa aacgttttat cagaactaaa tactttttta attagtgaaa
      301 agataaatac atcaatatgt tgaccaataa tatatgcgac aattgagcct aaagcaatac
      361 gtggcacaac atcaaagatt gcgtgtaatg ctttttgtgc catatcttct ggtgcaggaa
      421 taaaatgtaa tgacaattgc ataacaataa tcataattaa tgttgatgaa aagcctaacc
      481 aaactgctct ttttgcaact ctacgcccat aaatatcatt taaaatatct gttgctaaat
      541 aaatagaagc aaacatgaca ttacctaaag ttgctgaaat accaaagatt tctacagttt
      601 taatcacttg tatgttggca atgattgtgc caattgcaac ccatgcaatt aaaccttgtt
      661 taccaaaaaa gcgatacata agtaccataa gtacgaacgt tgcaataaac gtaactagtc
      721 ctaaaatttc attatacata ttaaaatgtc ctcctaaatt ttgatcatgc gggtgtttag
      781 aaaccgctca ataaataact tactcatttt actgaaaaat cgtttgcgat gcaatagcat
      841 ttgaatataa atacatttta cgtatagaat tgtaaaaggt tttattcaaa tcttagggtc
      901 aaaaatgtta taatattttt atgtcaaatt taaaacagta acacttattt acaaggttgc
      961 aatattttga agtaataaag gaagtgtcgc gtattttaac tttttcagag caaaatgcac
     1021 tcgcgaaaat agatgattta atgaatactt attgcaatca atgtccaatc aaaactcgtc
     1081 tgcgtaaatt agaggggaaa acgaaggcgc atcatttttg tatcaatgag tgttcaatag
     1141 ggaaagaaat aaaacaatta ggaaatgaac ttcaatagga ggaagtcaaa tgaaaattat
     1201 atctatatca gaaacaccga accacaacac aatgaagatt acacttagtg aaagcagaga
     1261 aggtatgaca tcagatacgt atactaaagt tgatgattca cagccagcat ttattaatga
     1321 catcttaaag gttgaaggcg ttaaatcaat tttccatgtt atggacttta tttcagtaga
     1381 taaagaaaat gacgcaaatt gggaaacagt attgccaaaa gtagaggctg tattcgaata
     1441 aatttttcat caactagtat tcggggggga ataaagtata tggaaatttt acgtatagag
     1501 ccaacaccaa gtccaaatac aatgaaagtt gttttgtcat atacaagaga agacaagtta
     1561 tctaatactt ataaaaaagt agaagaaaca caaccaagat ttataaatca gttgttatct
     1621 atagatggta tcacttccat ttttcatgtc atgaacttct tagctgttga taaggcacca
     1681 aaagctgatt gggaagtcat attacctgat attaaagctg ctttttctga tgcgaataag
     1741 gttttagaat ctgtaaatga acctcaaatt gacaatcatt ttggtgaaat taaagctgaa
     1801 ttattaactt ttaagggtat accgtatcaa attaagctaa cttctgctga ccaagaatta
     1861 agagaacaat taccacaaac atatgttgat catatgactc aagcgcaaac agcacatgac
     1921 aatattgttt ttatgcgtaa atggctagat ttaggaaatc gctatggaaa tattgaagaa
     1981 gtaatggatg gtgtcctaga agaagtgcta gctacctatc cagaatcaca gttacccgta
     2041 ttggtaaaac atgctttaga agaaaatcac gcaactaata attatcattt ctatcgacat
     2101 gtctctttgg atgaatatca tgcaactgat aattggaaga ctcgattacg aatgttaaac
     2161 cattttccaa agccgacttt tgaagatata ccgctacttg atttagcttt atctgatgaa
     2221 aaagtaccgg ttagacgtca agcgattgta ttattaggta tgattgaaag taaagaaatt
     2281 ttaccgtatt tatataaggg gcttcgtgat aaaagtcctg ctgtaagaag aacagcaggg
     2341 gattgcataa gcgatttagg gtatccagag gcactaccag aaatggtgct actattagat
     2401 gatccacaga aaatcgttag gtggcgtgct gctatgttta tctttgatga aggtaatgca
     2461 gagcagcttc ccgcactaaa agcccatatt aatgacaatg cgtttgaagt taaattacaa
     2521 attgaaatgg ccatatctcg tattgaaaac ggagatgagg cacttggttc agtatggaaa
     2581 caaatggcaa acagaacaat ttaattggag gaattaaata tgaatgcata tgatgcttat
     2641 atgaaagaaa ttgcgcaaca aatgcgtggc gaattaactc aaaatggttt tacaagttta
     2701 gaaacgagcg aagcagtatc ggagtatatg aaccaagtaa atgctgatga cactactttt
     2761 gtagttatta actctacatg cggctgtgca gctggattag caagaccagc tgcagtagca
     2821 gttgcaacac aaaatgaaca tagacctaca aatacagtta cagtttttgc tgggcaagat
     2881 aaagaagcaa ctgctacaat gcgagaattc attcagcaag caccatctag tccttcgtat
     2941 gctttattca aaggtcaaga tttagtttat tttatgccta gagaatttat cgaaggtaga
     3001 gatattaatg acattgcaat ggacttaaag gatgcctttg acgaaaattg taaatagtac
     3061 acataaataa atataaaggt taacacattt tataatatta aaaatgg
//
LOCUS       DAMZKC010000211         3002 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.214, whole genome shotgun sequence.
ACCESSION   DAMZKC010000211 DAMZKC010000000
VERSION     DAMZKC010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.214"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            265..1200
                     /gene="lukE"
                     /locus_tag="POB55_002441"
     CDS             265..1200
                     /gene="lukE"
                     /locus_tag="POB55_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_114304764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukED subunit E"
                     /protein_id="HDI6501380.1"
                     /translation="MFKKKMLAATLSVGLIAPLASPIQESRANTNIENIGDGAEVIKR
                     TEDVSSKKWGVTQNVQFDFVKDKKYNKDALIVKMQGFINSRTSFSDVKGSGYELTKRM
                     IWPFQYNIGLTTKDPNVSLINYLPKNKIETTDVGQTLGYNIGGNFQSAPSIGGNGSFN
                     YSKTISYTQKSYVSEVDKQNSKSVKWGVKANEFVTPDGKKSAHDRYLFVQSPNGPTGS
                     AREYFAPDNQLPPLVQSGFNPSFITTLSHEKGSSDTSEFEISYGRNLDITYATLFPRT
                     GIYAERKHNAFVNRNFVVRYEVNWKTHEIKVKGHN"
     gene            1202..2185
                     /gene="lukD"
                     /locus_tag="POB55_002442"
     CDS             1202..2185
                     /gene="lukD"
                     /locus_tag="POB55_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukED subunit D"
                     /protein_id="HDI6501381.1"
                     /translation="MKMKKLVKSSVASSIALLLLSNTVDAAQHITPVSEKKVDDKITL
                     YKTTATSDNDKLNISQILTFNFIKDKSYDKDTLVLKAAGNINSGYKKPNPKDYNYSQF
                     YWGGKYNVSVSSESNDAVNVVDYAPKNQNEEFQVQQTLGYSYGGDINISNGLSGGLNG
                     SKSFSETINYKQESYRTTIDRKTNHKSIGWGVEAHKIMNNGWGPYGRDSYDPTYGNEL
                     FLGGRQSSSNAGQNFLPTHQMPLLARGNFNPEFISVLSHKQNDTKKSKIKVTYQREMD
                     RYTNQWNRLHWVGNNYKNQNTVTFTSTYEVDWQNHTVKLIGTDSKETNPGV"
     gene            2427..2570
                     /locus_tag="POB55_002443"
     CDS             2427..2570
                     /locus_tag="POB55_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gallidermin/nisin family lantibiotic"
                     /protein_id="HDI6501382.1"
                     /translation="MEKVLDLDVQVKGNNNTNDSAGDERITSHLFCSFGCEKTGSFNS
                     FCC"
BASE COUNT         1143 a          399 c          502 g          958 t
ORIGIN      
        1 ttgattaaag ttcgttttga tttttatagc gaaaaagttg agtatcggta attttatcat
       61 attgttcgag tataaaggga actatttaaa tgtaatgaag gttgcatcaa aatgaccggt
      121 tttcagcagc ataaatgaat aaattgtgtc atttttaata ttttatagca ttttattttt
      181 taatgaacag ttaattttat aaataagtga aataatctag aataaatttg tattaaaaat
      241 ttatatagaa agaaagtgaa gcttatgttt aagaaaaaaa tgttagctgc aactttgtca
      301 gtaggactga ttgcaccttt agcatctccg attcaagaat ctagagcaaa tactaatatt
      361 gaaaatattg gtgatggtgc tgaagtaatc aaacgtacgg aggatgtaag tagtaagaaa
      421 tggggcgtta ctcaaaatgt ccaattcgac tttgtaaaag ataaaaaata taacaaagac
      481 gctttaattg ttaaaatgca aggttttatt aattccagaa cttcattttc agatgtgaag
      541 ggtagtggat atgaattaac taaacgaatg atttggccat tccaatataa tataggactg
      601 acgactaaag atccaaatgt tagcttaatc aattaccttc ctaaaaacaa aatagaaact
      661 actgatgttg gtcaaacatt aggatataac attggaggta atttccagtc agcaccatct
      721 ataggtggca atggctcatt taattattct aaaacaatta gttataccca aaagagttat
      781 gtcagtgaag tagacaagca aaactcaaaa tctgttaaat ggggtgttaa agcaaacgaa
      841 tttgttacgc ctgatggaaa aaaatctgcg catgatagat atttattcgt acaaagtcca
      901 aatggtccaa caggttcagc aagagaatat tttgctcctg ataatcaatt gccaccttta
      961 gttcaaagtg gctttaatcc atcgtttatc actacactat cacatgaaaa aggttcaagt
     1021 gatacgagtg aatttgaaat ttcatatggt agaaacttag atattacata tgcgacttta
     1081 ttccctagaa ctggtattta cgcagaaaga aagcataatg catttgtaaa tagaaacttt
     1141 gtagttagat atgaagttaa ttggaaaaca cacgaaatta aagtgaaagg acataattaa
     1201 tatgaaaatg aaaaaattag tcaaatcatc agttgcttca tcaattgcac tgcttttgct
     1261 atcgaataca gttgatgcag ctcaacatat cacacctgta agcgagaaaa aagtagatga
     1321 caaaatcact ttatacaaaa caacagcaac atctgataat gataaattga atatttctca
     1381 aattttaacg tttaatttca ttaaggataa aagttatgac aaagatacgt tagtacttaa
     1441 ggcagccgga aacattaatt caggttataa aaagcctaat ccaaaagatt acaattactc
     1501 acagttttat tggggcggta agtataatgt ttcggttagt tcagaatcaa atgatgctgt
     1561 aaatgttgtt gactatgcac ctaaaaatca aaatgaagaa ttccaagttc aacaaacatt
     1621 aggttattct tatggcggag atattaatat atctaatggc ttatcaggtg gattaaatgg
     1681 atcaaaatca ttttcagaaa cgataaatta taaacaagaa agttacagaa ctacgattga
     1741 tagaaaaaca aatcataaat caattggctg gggtgttgag gcgcacaaaa ttatgaataa
     1801 tggttgggga ccatatggta gagatagtta tgacccaaca tatggtaatg aactgttttt
     1861 aggtggtaga caaagtagtt caaatgctgg tcaaaatttc ttgccaacac atcaaatgcc
     1921 tttattggcg cgtggtaact ttaacccaga atttataagc gtactttctc ataaacaaaa
     1981 tgatacaaaa aaatctaaaa tcaaagtaac ttaccaaaga gaaatggata gatatactaa
     2041 tcaatggaat cgactacact gggttggtaa taactacaaa aatcaaaata cagtaacgtt
     2101 tacatctact tatgaagttg actggcaaaa ccatactgtt aaattaatcg gtacggattc
     2161 taaagaaact aatcctggag tataactgtt agaaatagag gaagttaaac ccgaaattta
     2221 agagttaaac aaaaaatatt cacaacatat taaaaaaaga agttacatta tttgtttaaa
     2281 ctaacaatag taccataaaa ttaatgacga tttacaaatt aataacaaat aattattatt
     2341 aaattcattg atttattgtg tggtaatgat taatatgaca attgaagtgg gtcttaactt
     2401 caacttttaa atagggggtt ttatctttgg aaaaagttct tgatttagac gtgcaagtta
     2461 aaggaaataa caacactaat gattcagcgg gtgacgaaag aataactagc catctttttt
     2521 gtagctttgg ttgtgaaaag acgggtagtt ttaacagctt ctgttgttaa ggatacagca
     2581 aatagagcct gaaagggctc tatttgtctt tttaaatata ttaagtgcta aaaaacggaa
     2641 acagtttatt aatcgccagg tatttttata attagccttc tatattatca gttgagtgtt
     2701 tcaataaatt ctgactttgg tgatggaaag ttatactttt agttgtaata tagttttcat
     2761 gacttcctgt taaataagtt aggattatgt aattagcctc tatctttaca tataagtaat
     2821 gacaagcttt ggttattata ttgaccctct tagaacatgt catttaagaa aataatcaag
     2881 tcattgaaat tatatgttta tatcctcatc tttaaaataa cttcaattat tatttgctaa
     2941 attttgtata aatagaacaa aactgcataa atatctatta attaatctca agcagccatc
     3001 tc
//
LOCUS       DAMZKC010000212         3000 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.215, whole genome shotgun sequence.
ACCESSION   DAMZKC010000212 DAMZKC010000000
VERSION     DAMZKC010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.215"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..186
                     /locus_tag="POB55_002444"
     CDS             <1..186
                     /locus_tag="POB55_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase"
                     /protein_id="HDI6501383.1"
                     /translation="SHYKEDYLVNFIDINENKQDVFKSRKVGLLEGELGVYLTLMTLK
                     NTKILNEKNWTNAFLIS"
     gene            202..720
                     /locus_tag="POB55_002445"
     CDS             202..720
                     /locus_tag="POB55_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HDI6501384.1"
                     /translation="MGENVLICLCGSVNSINISHYIIELKSKFDEVNVIASTNGRKFI
                     NGEILKQFCDNYYDEFEDPFLNHVDIANKHDKIIILPATSNTINKIANGICDNLLLTI
                     CHTAFEKLSIFPNMNLRMWENPVTQNNIRLLKDYGVSIYPANISESYELASKTFKKNV
                     VAPEPYKVLEFI"
     gene            730..2103
                     /locus_tag="POB55_002446"
     CDS             730..2103
                     /locus_tag="POB55_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HDI6501385.1"
                     /translation="MKIIKRAIISLIILSLLISITMSNASASEELYYSVEYKNTATFN
                     KLVKKKSLNVVYNIPELHVAQIKMTKMHANALANYKNDIKYINATCSTCITSEKTIDR
                     TSNESLFSRQWDMNKITNNGASYDDLPKHANTKIAIIDTGVMKNHDDLKNNFSTDSKN
                     LVPLNGFRGTEPEETGDVHDVNDRKGHGTMVSGQTSANGKLIGVAPNNKFTMYRVFGS
                     KKTELLWVSKAIVQAANDGNQVINISVGSYIILDKNDHQTFRKDEKVEYDALQKAINY
                     AKKKKSIVVAAAGNDGIDVNDKQKLKLQREYQGNGEVKDVPASMDNVVTVGSTDQKSN
                     LSEFSNFGMNYTDIAAPGGSFAYLNQFGVDKWMNEGYMHKENILTTANNGRYIYQAGT
                     SLATPKVSGALALIIDKYHLEKHPDKAIELLYQHGTSKNNKPFSRYGHGELDVYKALN
                     VANQKAS"
     gene            2126..2818
                     /locus_tag="POB55_002447"
     CDS             2126..2818
                     /locus_tag="POB55_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding subunit"
                     /protein_id="HDI6501386.1"
                     /translation="MAKLVTENISKRFKNQDVLKHINITLENNEVYGLLGINGAGKTT
                     LMKIICGILQQDSGEIKLDNRPMTRNDLHKVGSLIETPATYNHLSAQDNLKIVCLNES
                     VDFSEINSVLSLVNLNVDKKKKVKDFSLGMKQRLGIAMALIKKPEILVLDEPSNGLDP
                     YGIQELRELLKLLTEQGTSIIISSHILSEIQVLADHIGIIHEGELKYQQRNNKDENLE
                     EIFFKITKGDYK"
     gene            2815..>3000
                     /locus_tag="POB55_002448"
     CDS             2815..>3000
                     /locus_tag="POB55_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HDI6501387.1"
                     /translation="MIHLKIEGIKFKNSFSMYVLLISPLVFLCFAIFTVLFAKSNTGT
                     ANSVSPYITLLFNIWPIA"
BASE COUNT         1155 a          386 c          537 g          922 t
ORIGIN      
        1 tctcattata aggaagatta tctggtgaat tttatagaca ttaatgaaaa taagcaagat
       61 gtatttaaaa gtaggaaagt tggcctttta gaaggtgaat taggggtcta tctaacattg
      121 atgacactga aaaatacaaa aattctcaat gaaaaaaatt ggaccaatgc gtttttaatt
      181 agctgaatgg aggcgtatat catgggagaa aatgtattga tttgtttgtg tggctcagtt
      241 aatagtatca atattagcca ctatattatt gaattaaaat caaaatttga tgaagtaaat
      301 gtaatcgcgt caacaaacgg tagaaagttt attaatggtg aaatattaaa gcaattttgc
      361 gataactatt atgacgaatt tgaagaccct tttttaaatc atgtagatat agcaaataaa
      421 catgacaaga ttattatttt acctgcgact tctaatacga ttaataaaat tgcaaatggt
      481 atatgtgaca atttattatt aactatttgt catacagctt ttgaaaaact ttctatattt
      541 ccaaatatga atttacgaat gtgggaaaat ccagttactc aaaataatat tcgattatta
      601 aaagattatg gtgtatcaat atatccagca aatatttcag aaagttatga attagcgtca
      661 aaaacattta aaaagaatgt tgtcgcacct gaaccatata aagttctgga attcatttga
      721 gattagaata tgaaaatcat aaagcgcgct attattagtt taataatatt gagtctttta
      781 atatctatta caatgagtaa tgcatcagcg tcagaagaac tatattacag tgttgaatac
      841 aaaaatacag caacatttaa caagttagtt aaaaagaagt ccttaaacgt tgtctataat
      901 ataccggaat tacatgtggc acagattaaa atgacgaaaa tgcatgctaa tgctttagca
      961 aactataaaa atgatattaa atatatcaat gccacatgtt caacttgtat tactagcgag
     1021 aaaacaatag acagaacatc aaatgagtca ttattttcaa gacaatggga tatgaataaa
     1081 ataaccaata atggtgcatc gtatgatgat ttgccaaaac atgctaacac caaaatagca
     1141 atcatagata caggtgtgat gaaaaaccat gacgatttga aaaataattt ctcgactgat
     1201 tctaaaaatt tagtaccttt aaacggtttt agaggtactg aaccggagga aacaggtgat
     1261 gttcacgatg tcaatgatag gaaaggacat ggcacgatgg tgtcgggtca aacgagtgct
     1321 aatggtaagt taataggtgt tgcaccgaat aacaaattta caatgtatcg cgtgtttggt
     1381 agtaaaaaaa cagaactgct ttgggtatca aaagcgattg ttcaagctgc aaatgatgga
     1441 aatcaagtca ttaatattag tgttggtagt tatattattt tggacaaaaa tgaccatcaa
     1501 acatttagaa aagatgaaaa agtagaatac gatgcgttac agaaagcaat caattacgcc
     1561 aagaagaaaa aatctatcgt tgttgctgca gctggtaatg atggtattga tgtcaatgac
     1621 aaacagaaac taaaattaca gcgtgaatat caaggtaatg gcgaagtgaa agatgttcct
     1681 gcatctatgg acaatgtcgt tacagtagga tctacagatc aaaagagtaa tctatctgag
     1741 ttttccaatt ttggtatgaa ttatacagat attgctgcgc ccggaggatc atttgcttat
     1801 ttaaatcaat tcggtgtgga taaatggatg aatgaagggt atatgcataa ggagaacatt
     1861 ttaactactg ccaataacgg aagatatatt tatcaagctg gaacttcatt agccacacct
     1921 aaagtttcgg gagcactagc tttaatcatt gataaatatc atcttgaaaa acatccagat
     1981 aaagcgattg aattgttata tcagcatggg acatctaaga ataataaacc atttagtaga
     2041 tatgggcatg gtgagcttga tgtgtataaa gcattaaatg tagcaaatca aaaagcaagt
     2101 taataaatca aaggagtttt tgattatggc aaaattagtt actgaaaaca tttcgaaacg
     2161 gtttaaaaat caagatgtat taaagcatat taatatcact ttagaaaata acgaagttta
     2221 tggattactt ggtattaatg gagccggtaa aacgacactt atgaaaatta tatgtggcat
     2281 acttcaacaa gattcagggg aaattaaatt agataataga ccaatgacac gaaatgattt
     2341 gcacaaagtt ggttcgctta ttgaaacacc tgcgacatat aatcatttaa gtgcacaaga
     2401 taatttgaaa attgtgtgtt taaatgaaag cgttgatttc agcgaaatta atagtgtttt
     2461 aagcttagtc aatttaaatg tcgataaaaa gaaaaaggtt aaggactttt ctttaggtat
     2521 gaaacaaaga cttggaattg caatggcatt aattaaaaag ccagaaattt tagtattaga
     2581 cgaaccatct aatggtttag acccatatgg aatccaagaa cttagagaac ttctaaaatt
     2641 attaacagaa caaggtacta gtattattat ttcaagtcac attttatctg aaatccaagt
     2701 tttagcagat catatcggta ttattcatga gggtgagcta aaatatcagc aaagaaataa
     2761 caaagatgaa aacttagaag agatattctt caaaataacg aaaggtgatt acaaatgata
     2821 catttaaaga tagaaggtat caaatttaaa aattctttca gtatgtatgt tttattaata
     2881 agtccgctgg tatttctttg ttttgctatt ttcacagtct tattcgccaa aagtaatacg
     2941 ggaacagcga atagtgtgtc accatatata actttactat ttaatatttg gccaattgct
//
LOCUS       DAMZKC010000213         2989 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.216, whole genome shotgun sequence.
ACCESSION   DAMZKC010000213 DAMZKC010000000
VERSION     DAMZKC010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.216"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(96..962)
                     /gene="fakB1"
                     /locus_tag="POB55_002449"
     CDS             complement(96..962)
                     /gene="fakB1"
                     /locus_tag="POB55_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase binding subunit FakB1"
                     /protein_id="HDI6501388.1"
                     /translation="MKIAVMTDSTSYLSQDLIDKYNIQIAPLSVTFDDGKNFTESNEI
                     AIEEFYNKMASSQTIPTTSQPAIGEWITKYEMLRDQGYTDIIVICLSSGISGSYQSSY
                     QAGEMVEGVNVHAFDSKLAAMIEGCYVLRAIEMVEEGYEPQQIIDDLTNMREHTGAYL
                     IVDDLKNLQKSGRITGAQAWVGTLLKMKPVLKFEDGKIIPEEKVRTKNRAIQTLEKKV
                     LDIVKDFEEVTLFVINGDHFEDGQALYKKLQDDCPSAYQVAYSEFGPVVAAHLGSGGL
                     GLGYVGRKIRLT"
     gene            1106..1747
                     /locus_tag="POB55_002450"
     CDS             1106..1747
                     /locus_tag="POB55_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HDI6501389.1"
                     /translation="MTQNIITVKKEHIIENVISKSRFIAHIKPVQNEDEAKAFIAAIK
                     KEHKDATHNCSAYTIGPEMNIQKANDDGEPTGTAGVPMLDILKKLDVHNACVVVTRYF
                     GGIKLGGGGLIRAYSGAVRDVIYDVGRVELREAVPCIVTLNYDQTGKFEYELASTHFM
                     LRNQTYTDKVSYYIDVVKSDYEAFINFLNQMTAGNFDLTEETPKQLPFDIPTE"
     gene            complement(1919..2974)
                     /locus_tag="POB55_002451"
     CDS             complement(1919..2974)
                     /locus_tag="POB55_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HDI6501390.1"
                     /translation="MVTLLLVAVTMIVSLTITPIVIAISKRLNLVDKPNFRKVHTKPI
                     SVMGGTVILFSFLIGIWIGHPIETEIKPLIIGAIIMYVLGLVDDIYDLKPYIKLAGQI
                     AAALVVAFYGVTIDFISLPMGTTIHFGFLSIPITVIWIVAITNAINLIDGLDGLASGV
                     SAIGLITIGFIAILQANIFITMICCVLLGSLIGFLFYNFHPAKIFLGDSGALMIGFII
                     GFLSLLGFKNITIIALFFPIVILAVPFIDTLFAMIRRVKKGQHIMQADKSHLHHKLLA
                     LGYTHRQTVLLIYSISILFSLSSIILYVSPPLGVVLMFVLIIFSIELIVEFTGLIDNN
                     YRPILNLISRKSSHKEE"
BASE COUNT         1067 a          525 c          437 g          960 t
ORIGIN      
        1 tagcattttg gctttcaaat gatgaacatt taaagccatg tatacgagag ataatttcca
       61 attacaatat agactctttt attaaaattt tataattatg ttaatcttat ttttctgcca
      121 acatagccta aacctaatcc accagaacct aaatgtgctg caacaactgg accaaactca
      181 gagtatgcta cttgataagc tgaaggacaa tcatcttgta actttttgta taacgcttga
      241 ccatcttcga aatgatctcc atttatgaca aataaagtta cttcttcaaa gtcttttaca
      301 atatctaata ctttcttttc taatgtttga atggcacgat ttttagtacg aactttttct
      361 tctggtataa tcttgccatc ttcaaactta agaactggct tcattttcaa taatgttcca
      421 acccatgctt gagcaccagt aattcgacca cttttttgta aattctttaa gtcatcaaca
      481 attaaatatg cacctgtgtg ttcacgcata ttagttaaat catcaataat ttgctgtggt
      541 tcgtatcctt cttcaaccat ttcaatagca cgtaatacat aacatccttc aatcattgct
      601 gcaagcttac tatcaaatgc atgtacatta acaccttcaa ccatttcccc tgcttgataa
      661 ctagattgat aacttccact aatcccactg gataagcaaa tgacaatgat atctgtgtaa
      721 ccctgatctc ttagcatttc atatttagta atccattcgc caattgctgg ttggcttgtt
      781 gttggaatcg tttgagacga tgccatttta ttataaaatt cttcaattgc tatttcatta
      841 ctttctgtaa agttcttccc atcatcaaaa gtcacactta atggcgctat ttgaatatta
      901 tatttatcga ttaagtcctg cgacagataa cttgtagaat cggtcatcac agcaattttc
      961 atagtcgtcc tcctaaaata tactcatagc aatgatacaa tatttcagtt aaaaagaaaa
     1021 tggcaatcaa acgatttata gtcatgtcat tttcagtgta taatgaagat taaataatct
     1081 taagcttatg aattaaggag ttattatgac acaaaatatc attactgtta aaaaagaaca
     1141 cattattgaa aatgtgatta gtaaatcaag attcattgct catatcaaac ctgttcaaaa
     1201 tgaagatgaa gctaaagcat ttattgctgc aataaaaaaa gaacataaag atgccactca
     1261 taattgttct gcatatacta ttggacctga aatgaatatt caaaaagcga atgatgacgg
     1321 tgaaccgaca ggaactgccg gtgtcccaat gttagacata ttaaaaaagc tcgatgttca
     1381 taatgcgtgt gtcgtagtaa caagatattt tggtggtatt aaattaggtg gtggcggttt
     1441 aattcgcgcc tatagcggtg ctgttagaga cgtcatttat gatgtcggac gtgtcgaact
     1501 acgagaagca gtaccatgta tagtaacact gaattatgat caaactggta aatttgaata
     1561 tgaattagct tcaactcatt ttatgttaag aaaccaaact tacactgata aggtaagcta
     1621 ctatatcgat gtcgttaaaa gtgactatga agcgtttatt aactttttaa atcaaatgac
     1681 tgcaggaaat ttcgatttga ctgaagaaac tccaaagcag ttacctttcg atataccaac
     1741 tgaataaaaa taaaaagcac tatcataaga cggtaaatat caattgcgta ttattaaacg
     1801 acaattgaag tacacgttta tggcagtgct tttttatatt tgaaatttaa tacataaaaa
     1861 attgcaaaga gtaaagccat ataatacaaa ttatcccata cagctatgct ttcattccct
     1921 attcctcttt gtgtgacgat ttccgactaa ttaaatttaa tattggtcgg tagttgttat
     1981 ctattaatcc tgtaaattca acaattaatt caatactaaa tatgattaat acaaacatta
     2041 atacaacacc taatggtggc gatacataca aaataatgct cgaaagacta aataaaatag
     2101 agattgaata gattaataat actgtttgtc tatgtgtgta gcctaaagct aatagtttat
     2161 gatgcaaatg cgatttatca gcttgcatta tatgctgccc ttttttcaca cgtcgaatca
     2221 ttgcgaacaa agtatcaatg aatggaactg ctaagataac aattgggaag aacaatgcaa
     2281 taattgtaat atttttgaat ccgagtaaag aaaggaatcc gatgataaat ccaatcatta
     2341 aagccccact atcacctaaa aatattttgg caggatggaa attgtaaaat aaaaacccaa
     2401 ttaaagagcc taataaaaca caacaaatca tcgttatgaa aatattagct tgtaaaattg
     2461 caatgaatcc tattgtaatg agtccgattg cagaaacacc cgacgccaaa ccatcgagtc
     2521 catcaattaa gttaattgca tttgtaatag caacaatcca aatcacagta attggaatac
     2581 taagaaatcc aaaatgaatc gttgtaccca ttggcaacga aataaaatca atagtcacac
     2641 cataaaaagc aactactaag gcagcggcaa tttgaccagc caattttata tacggtttca
     2701 aatcgtagat atcatctaca agcccaagta cgtacataat aatcgcacca ataataagtg
     2761 gtttgatttc tgtttcaata ggatgaccaa tccaaatacc tattaaaaat gaaaagagaa
     2821 tcactgtacc acccataact gaaataggtt tagtgtgtac ttttctaaaa tttggtttat
     2881 caactaaatt taatcttttc gatattgcaa taacaattgg tgttatcgtc aaactgacaa
     2941 tcattgttac tgcaactagt aataatgtaa ccatttattc accttcatc
//
LOCUS       DAMZKC010000214         2978 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.217, whole genome shotgun sequence.
ACCESSION   DAMZKC010000214 DAMZKC010000000
VERSION     DAMZKC010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.217"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(355..720)
                     /locus_tag="POB55_002452"
     CDS             complement(355..720)
                     /locus_tag="POB55_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDI6501391.1"
                     /translation="MKASRILFGIGVGVAAGFVVALQGRDDKSVKNNTIDRTAPTGSK
                     SELQREFETIKQSFNDILNYGVQIKNESAEFGSSIGGEIKSLLGNFKSDINPNIERLQ
                     SHIENLQNRGEDIGNEISK"
     gene            complement(862..1284)
                     /locus_tag="POB55_002453"
     CDS             complement(862..1284)
                     /locus_tag="POB55_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HDI6501392.1"
                     /translation="MSETIFGKILTGEIPSFKVYEDDYVYAFLDISQVTKGHTLLIPK
                     KASANIFETDEETMKHIGAALPKVANAIKRAFNPDGLNIIQNNGEFADQSVFHIHFHL
                     IPRYENDIDGFGYKWETHEDILDNDAKQQIAEQIQAQF"
     gene            1418..2158
                     /locus_tag="POB55_002454"
     CDS             1418..2158
                     /locus_tag="POB55_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6501393.1"
                     /translation="MTVKVEQLTGGYGKRPVIKDINFELNKGEIVGLIGLNGAGKSTT
                     IKHMLGLLTPMEGSLSISDININDDIEAYRRKLSYIPESPVIYEELTLEEHIEMTAMA
                     YDIDRDEAMNRAMPLLKTFRLENELKVFPSHFSKGMKQKVMIICAFIVNPELYIIDEP
                     FLGLDPLGIQSMLDLMVEKKNEGRTVLMSTHILATAERYCDRFIILDEGEVVAFGDLE
                     ALRQQTGLHKQTLDDIYIHVTQGGDVHA"
     gene            2151..>2978
                     /locus_tag="POB55_002455"
     CDS             2151..>2978
                     /locus_tag="POB55_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6501394.1"
                     /translation="MHNQATTLFNKRLHALRKEKNYYNKFIFNGHFMVFLLILLGAFI
                     FGYGEWLKHIPTNINFSLIAAVIVALTSIFPMRPLLKEADKIFLLPFEKHMSQFMRHA
                     ILYSYFARILIQLIIVIVMFPLFYNINQHNVAFYICFGVSALIFPYVGLRLRWQWYQS
                     GLKTWQVNLISFITFALTYYLLLAPKWYIAFVMVALPVLIEFLVKKYKPGFLYPWEKM
                     IAIEHRHHMNYYKFVNMFTDVKHLKESAVRRSYLDILLPVPKGSKFNSNAMYLFLFIR
                     "
BASE COUNT          991 a          454 c          490 g         1043 t
ORIGIN      
        1 cagattaata atttgtgcaa gtaaggtata tgtaatgtgc ataaaaggtt cggctcaatg
       61 aaaatataag tatgttgttt gtcgataatg atttaagtaa agcgtaatca aagtgcaagg
      121 ataacacaat gaatcctaag caatgttttc aatcaattca taaatatgat ttaagcgaaa
      181 atcactacaa acgatgtatt cctgtaattt gtaaacataa gttcatttga gttataaaaa
      241 agagatgaca agaaatatct aatttctaat gtcatctcgt ttatttggca tttaattaaa
      301 aaaaataaat atgctgttac taataataat agttgtgatc gagaacgtaa cgtgctactt
      361 agaaatttcg tttccaatat cctcgccacg attttgtaaa ttttcgatgt gtgactgtaa
      421 acgttcaata ttaggattaa tgtcagattt gaagtttcca agtaatgact taatttcacc
      481 accaattgaa ctaccaaatt ccgcactttc gtttttaatt tgaacaccat agtttaaaat
      541 gtcattaaaa ctttgtttaa tcgtttcaaa ttcacgttgt agttctgatt ttgaaccagt
      601 aggggcagta cgatcgatcg tgttgttctt gacacttttg tcgtctcgtc cttgaagtgc
      661 aactacaaaa ccagctgcta cgccaacacc gataccgaat agaatgcgtg atgctttcat
      721 ttatattctc ctcactttta acatctgtta tcttgataac tcattttgtt taaaattgct
      781 aaccttgatg ttatcacttc aaacgcaatt gttataatat taatcatatt atacccgttc
      841 gagcttagat taagcataca tttaaaattg tgcttgaatt tgttcagcaa tttgttgttt
      901 tgcatcgtta tctaaaatgt cttcatgtgt ttcccactta taaccaaatc catcaatatc
      961 attttcatat cgaggaatta agtggaaatg aatatgaaat acagattgat ctgcaaactc
     1021 accattattt tgaataatgt ttaaaccatc aggattaaat gcacgcttaa tagcatttgc
     1081 tactttaggt aatgctgcac cgatatgttt cattgtttct tcatcagttt caaagatatt
     1141 agcagaagct tttttaggaa ttaataacgt atgtccttta gtaacttgtg atatatctaa
     1201 aaaggcatag acataatcgt cttcatatac tttaaagcta ggaatttctc cagttaaaat
     1261 tttgccgaaa attgtttctg acatgatgac tacactcctt ttcaacttgt attaccattc
     1321 attataacat tgtcgctaaa aattcgatat taggacatgt gtatttaata tgaggatttt
     1381 ataatttttg gtacaatata agtcatggag gtgccttatg acagttaaag tagaacagct
     1441 tacaggtgga tatggaaaac gccctgtaat taaagatata aattttgaat taaacaaagg
     1501 cgaaatcgtt ggacttattg gccttaatgg cgctggtaag agtacaacga ttaaacatat
     1561 gctaggattg cttacaccta tggaaggatc tctatcaatt tcagatatca acattaatga
     1621 tgacatagag gcttatagaa gaaagttatc ttatattccg gaatcaccgg ttatttatga
     1681 agaactcaca ttagaggaac acattgagat gacagcaatg gcatatgata ttgatcgtga
     1741 tgaagcgatg aatcgagcaa tgccattatt aaagacattc cgtttagaaa atgaattgaa
     1801 agtttttccg agtcattttt ctaaggggat gaaacaaaaa gtcatgatta tttgtgcgtt
     1861 tatcgttaat cccgaattgt atattataga cgaaccattc cttggcctag atccattagg
     1921 aattcaatcc atgttagatt taatggttga aaagaaaaac gaaggtagaa ccgttctaat
     1981 gagtacacat attttagcaa cagctgaacg ttattgtgat cgttttatca tactagacga
     2041 aggtgaagtc gttgcatttg gagatttaga agcattgaga caacaaactg gcttacataa
     2101 acaaacatta gacgatatat atattcatgt gacgcaaggt ggagatgtac atgcataacc
     2161 aagcgaccac attatttaat aaaagattac atgcccttcg taaagagaaa aattattata
     2221 ataagtttat atttaatggt catttcatgg tctttttact tattttgctg ggtgcattta
     2281 tttttggata tggtgaatgg ttaaaacata ttccaacgaa tatcaatttt tcattaatag
     2341 cagcagtgat tgtagcattg acatccattt ttccaatgcg accactccta aaagaagcgg
     2401 ataaaatctt tttattacca tttgaaaaac acatgtctca atttatgcgt catgcgattt
     2461 tatatagtta ctttgcgcgc atattaatcc aactcattat tgttattgta atgttcccgt
     2521 tgttttacaa tattaatcaa cataatgtgg cattttatat ttgttttgga gtcagtgcat
     2581 taatttttcc atatgttggt ttacgcttaa gatggcaatg gtatcaatcg ggacttaaaa
     2641 catggcaagt taatttaata tcattcatca cctttgctct aacatattac ttactattag
     2701 ctccaaaatg gtacatcgca tttgtgatgg ttgctttgcc tgtgttaata gaatttttag
     2761 ttaaaaaata taaaccaggc tttttgtatc cttgggagaa aatgattgca atcgaacatc
     2821 gacatcatat gaattattat aaatttgtta atatgttcac cgatgtaaaa catttgaaag
     2881 aatctgcagt cagaagaagt tatctagata tacttttacc agttccaaag ggtagtaaat
     2941 ttaactctaa tgcgatgtat ttattcttat ttattaga
//
LOCUS       DAMZKC010000215         2964 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.218, whole genome shotgun sequence.
ACCESSION   DAMZKC010000215 DAMZKC010000000
VERSION     DAMZKC010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.218"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(<1..507)
                     /locus_tag="POB55_002456"
     CDS             complement(<1..507)
                     /locus_tag="POB55_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501395.1"
                     /translation="MSDRFIKFNDEQLDAKQVMMLQDLARLLLKNEQTQVKIQKFPYY
                     NPVQNVLITSWFWSHRPSHIEMAGLKTDVMLAAYGYHMMDVQIVNEVVQDKTFKHPKF
                     YQQLFKLLEDMRVLNSIKVERPSTAKLIDLRLDTRISYTESQIKVYRTKTQYTDLLFL
                     YLEHAFLSQ"
     gene            complement(521..1312)
                     /locus_tag="POB55_002457"
     CDS             complement(521..1312)
                     /locus_tag="POB55_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HDI6501396.1"
                     /translation="MALKHYKNSDSTVFNDAKALFDLNKNILLKGPTGSGKTKLAETL
                     SEVVDTPMHQVNCSVDLDTESLLGFKTIKTNAEGQQEIVFVDGPVIKAMKEGHILYID
                     EINMAKPETLPVLNGVLDYRRQITNPYTGEVIKAVPGFNVIAAINEGYVGTLPMNEAL
                     KNRFVVIHVDYIDGDILKNVIKEQSLLQDDKQIEQIIKFNEDLRTMSKQGQISEEAAS
                     IRALLDLCDLITVMPVERAIKRTIIDKLEDEREQQAIYNAVELNF"
     gene            complement(1493..1696)
                     /locus_tag="POB55_002458"
     CDS             complement(1493..1696)
                     /locus_tag="POB55_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501397.1"
                     /translation="MLHETWKERTPIKKVEVINTDAKKFTVSDMLTVGKQYDVINETE
                     EYYQIIDNSGLVGGYYKTYFKEV"
     gene            complement(1725..2534)
                     /locus_tag="POB55_002459"
     CDS             complement(1725..2534)
                     /locus_tag="POB55_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDI6501398.1"
                     /translation="MCGLRSITLGTTNIEQTKHFMVDILGLNYEELLENSIRFGDADI
                     SPGTRLQFIQVPSEQLEESHFVGIGLRTPTDSGLEEYAEILSNKDIPFTTVKELNGNK
                     YFSLEDNNGHIFSIYSNENNYGVGLGMPSFESAVNPLHQVQGLGPVILKVNHVDITGQ
                     ILTNIFGLEVFAEYQPFDNADYHVQVFKVGTGGLGGEIHLMPVETEMTMPEYGAVDQV
                     EFETKDADFFNQAKSRLDEVEIPYQTLEQDDIESIRITENSGLSFIFTLQK"
BASE COUNT          926 a          505 c          403 g         1130 t
ORIGIN      
        1 ttggcttaaa aatgcatgtt ctaaatacaa aaataataaa tctgtatatt gtgttttagt
       61 acgatacact ttaatttgag attccgtata tgatatacgt gtatctaagc gaagatcaat
      121 taatttagca gtacttgggc gctcaacttt aatagaattt aatacgcgca tatcttctaa
      181 taatttaaaa agttgttgat aaaatttagg gtgtttaaaa gttttatctt gtactacttc
      241 atttacaatt tgtacatcca tcatatgata accgtaagca gctaacataa catctgtttt
      301 taaaccagcc atttcgatat ggcttggtcg atgtgaccaa aaccaactcg taatcaaaac
      361 attttgaaca ggattatagt aagggaattt ttggatttta acttgtgttt gttcattttt
      421 taataagagt cgagctaagt cttgcaacat cattacttgc ttggcatcta actgttcgtc
      481 attaaatttt atgaaacgat cactcatgtt ttatccctct ttaaaagttt agttctacag
      541 cattatatat tgcttgttgt tcacgttcat cttccaattt atcaataatt gtacgtttaa
      601 ttgcacgttc aactggcatt acagtgatta aatcacacaa gtctaataat gcacggatac
      661 tagcggcttc ttcagaaatt tgtccctgct tagacatagt acgtaaatct tcattaaact
      721 taataatttg ttcgatttgt ttatcatctt gtaataaact ttgctcttta atcacatttt
      781 ttaaaatgtc cccatcaata taatcaacgt gaataacaac aaagcgattt tttagtgctt
      841 cattcattgg caaagtacca acataacctt catttatcgc tgctataacg ttaaatcctg
      901 gtacagcttt gattacttca ccagtgtatg gattcgtaat ttgacgacga taatctaaga
      961 ccccatttaa tacaggcaat gtttcaggtt tagccatatt tatttcatca atatataaaa
     1021 tatgcccctc tttcatagct ttaataactg gaccatctac aaagacaatt tcttgttgac
     1081 cttccgcatt tgttttaatt gttttaaagc ctaataagct ttctgtatct aaatcaacag
     1141 aacaattgac ttgatgcatg ggtgtatcaa caacttcact taatgtttct gccaactttg
     1201 ttttccctga acctgttgga cctttaagta aaatattttt atttaaatca aataatgcct
     1261 tcgcatcatt gaaaactgtt gaatctgaat tcttataatg ttttagtgcc attttaaaac
     1321 gttctccttt aaataaatta aaaaactaag acatggaata agttctccaa atgccttagt
     1381 ttactattct attctgatat aaaacgcaat gcttagtatg cgaattgttt atattttacc
     1441 aaatttacta attgaaaatc tcatattagg cttcatttca ataattatgc gtttaaactt
     1501 ctttgaaata tgttttatag taaccgccaa ctaatccaga attatctatt atttgataat
     1561 attcttcagt ttcgttgata acatcatatt gtttgccaac cgttaacatg tcagaaactg
     1621 taaatttctt tgcatctgta ttaatgactt ctactttctt gattggtgta cgttctttcc
     1681 aagtttcatg taacataaat tttgtcctcg ctatcgtaaa aaaattattt ttgtaaagtg
     1741 aatataaacg ataatccact gttttcagta attctaattg attcaatatc atcttgctca
     1801 agcgtttgat atggtatttc cacttcatct aagcgcgatt tcgcttgatt aaagaaatct
     1861 gcatctttcg tttcaaactc aacttgatcg actgcgccat attccggcat tgtcatttcg
     1921 gtttctacag gcattaaatg tatttcgcca cctagaccac ccgttccaac tttgaatact
     1981 tggacatgat agtcagcatt gtcgaagggt tggtattctg caaatacttc aagtccgaat
     2041 atatttgtta aaatttgacc tgtaatatct acatgattca ctttaagaat cactggtcct
     2101 aaaccttgca cttgatgtaa cggattgacc gcactctcaa aagaaggcat acctaaacca
     2161 acgccataat tattctcgtt tgaatatatt gagaaaatat gaccattgtt atcttcgaga
     2221 ctgaaatatt tattgccatt taattcttta actgttgtaa atggaatatc cttattcgat
     2281 aatatttccg catactcctc taaacctgag tcagttggtg tacgtaatcc aatacccaca
     2341 aagtgagatt cttctaattg ctcacttgga acttgtataa attgaagtct tgttcctggg
     2401 cttatatctg catcgccgaa acgaattgag ttttcaagaa gttcttcata atttaatcct
     2461 aatatgtcaa ccatgaaatg ttttgtctgt tctatatttg ttgtacctaa tgttatactt
     2521 ctaagtccac acattatagc cacctctatc tttatcttta ctattatatt ataacttatt
     2581 ttaatcataa attatagaat atcggtcagt tgattttgat tatagattaa tatgtgaaaa
     2641 atgtatttct ttaaaagaac tatttcattt aaataggtaa cataatattg ttatggttgc
     2701 tattgagtct gtcattaatg agtatctgta tctagcaatt gaatatttcg ttttttgtag
     2761 tacataaaaa acatatatat gtcgtgttca taaagtgaat ttacatcttg ttcagatgat
     2821 tttaaattaa ctaaaggcgc ttatagaatt cataaatttc atcgcttgtt attatctaaa
     2881 atgatttcat tagcatattg taacacgcta ttctacctat atttctattg tgagtctatt
     2941 tcaatagaaa tgatatattt ttta
//
LOCUS       DAMZKC010000216         2932 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.219, whole genome shotgun sequence.
ACCESSION   DAMZKC010000216 DAMZKC010000000
VERSION     DAMZKC010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.219"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            6..641
                     /locus_tag="POB55_002460"
     CDS             6..641
                     /locus_tag="POB55_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDI6501399.1"
                     /translation="MKLNNYSLKVKNKQLVDNCDLNFYLGQINHIVGKNGVGKSLLAK
                     DFLLNNSGNIPKSISQNVTLISSSSNIPNDITKDFLLSLLKSKFENNRQTFDKIYNIL
                     NIEAIPSNVLLKNLSDGQKQKLKLLSFLLEDHDLIILDEVTNALDKKTVNEIYEFLND
                     FIQSHQTKTIINITHNLSDLSALPGKYFIFKDLQIEEYQSKEEVINDYINL"
     gene            647..1378
                     /locus_tag="POB55_002461"
     CDS             647..1378
                     /locus_tag="POB55_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease"
                     /protein_id="HDI6501400.1"
                     /translation="MSTLKLEFKKSISNKIIYTLGVLFIFLFLLGYFLPIGIDKVKSL
                     SYSQFFFSSYTVATQLGFLLFSFVIAYFINKEYSNKNTLFYKLIGDNIFTFFYKKVAV
                     LFFECLVFIILSITIISIIYSDFSHYLLLIILFSLVILQYILVVGTISMVSPNILISL
                     GISIVYWIGSVILVAINKNIFGIVAPFEASNTMYRAVEKILNNESTYMCPTEIINTVS
                     FFVLLFIVNTIVLLLSRKRWLKIGM"
     gene            complement(1540..2160)
                     /gene="srtA"
                     /locus_tag="POB55_002462"
     CDS             complement(1540..2160)
                     /gene="srtA"
                     /locus_tag="POB55_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase SrtA"
                     /protein_id="HDI6501401.1"
                     /translation="MKKWTNRLMTIAGVVLILVAAYLFAKPHIDNYLHDKDKDEKIEQ
                     YDKNVKEQASKDKKQQAKPQIPKDKSKVAGYIEIPDADIKEPVYPGPATPEQLNRGVS
                     FAEENESLDDQNISIAGHTFIDRPNYQFTNLKAAKKGSMVYFKVGNETRKYKMTSIRD
                     VKPTDVEVLDEQKGKDKQLTLITCDDYNEKTGVWEKRKIFVATEVK"
     gene            2354..2845
                     /locus_tag="POB55_002463"
     CDS             2354..2845
                     /locus_tag="POB55_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDI6501402.1"
                     /translation="MIRYAKKEDLNAILAIYNDAIINTTAVYTYEPQTIDERVAWFET
                     KQRKHEPIFVFEENGSVLGFATFGSFRPWPAYLYTIEHSIYVDASARGKGIASQLLHH
                     LIVEAKAKGYRALVAGIDASNKASIQLHQKFAFKHAGTLTNVGFKFNQWLDLAFYELD
                     LQD"
BASE COUNT          973 a          473 c          412 g         1074 t
ORIGIN      
        1 tatacatgaa actaaataac tattctttaa aagttaaaaa caaacaacta gttgacaatt
       61 gtgatttaaa tttctatctt ggtcagatca atcacattgt tggtaaaaat ggtgtaggaa
      121 aatctttatt agctaaagat ttcttactaa ataatagtgg aaatatccct aagtccattt
      181 ctcaaaatgt aaccttaata tcaagttcat caaatattcc taatgatata acaaaagatt
      241 ttttattatc attgttaaaa tcaaaatttg aaaacaatcg acaaacattc gataagattt
      301 ataacatact aaacatcgaa gcaataccgt ctaacgtact actaaaaaac ttgagtgatg
      361 gtcaaaaaca aaagcttaaa ttattaagtt tcttattgga agatcatgat ttaatcatat
      421 tagatgaagt tacaaacgct ttagacaaga aaacagttaa tgaaatttat gaatttttaa
      481 atgattttat tcaaagccat caaactaaaa ctattatcaa tattacacat aatttatccg
      541 atttaagtgc tttgccgggt aaatacttta tttttaaaga ccttcaaata gaagagtacc
      601 aatcaaaaga agaagtcata aatgattaca ttaatttata atttaggtgt ccactttgaa
      661 attagaattt aaaaaaagta tttccaataa aattatttac acgcttggtg tgctattcat
      721 attccttttc ttacttgggt attttttacc aataggaata gacaaagtta aaagtttaag
      781 ctatagccag tttttcttca gttcatatac tgttgcaacg caattagggt tcttactatt
      841 ttcatttgtt attgcatatt ttattaataa agagtattcg aataaaaata ccctgtttta
      901 taaattgatt ggcgataata tctttacctt cttctataaa aaggtagcag tgttattttt
      961 tgaatgttta gtgttcataa ttctaagtat cacaattatt tcaattatat attcggattt
     1021 ctcacattac ttactattaa taatcttatt ttcactcgtt atcttacaat atatcttagt
     1081 tgttggaacg attagtatgg tttcacctaa tattttaatc agcttaggta tcagcattgt
     1141 atattggatt ggttcagtca tcttagttgc cattaataaa aatatatttg gtatcgttgc
     1201 tccatttgag gcgagcaata cgatgtatcg tgccgtagaa aaaatattga ataacgagtc
     1261 aacatatatg tgtcctactg aaattataaa tacagtatca ttcttcgttc ttctatttat
     1321 agttaatact attgtcttat tactaagtag aaagagatgg ttgaaaatcg gtatgtagta
     1381 cataaaaagg agcggcatcc attgtcgctc ctttttatta tgtattttta caatctccac
     1441 caatgacacg tcgtcattgc tacctcatac caaacaaaaa aacagtctcg caatcaagac
     1501 tgttttccac tcaatatatt catccattag cgtaatagat tatttgactt ctgtagctac
     1561 aaagatttta cgtttttccc aaacgcctgt cttttcattg taatcatcac aagtaattaa
     1621 tgttaattgt ttatctttac ctttttgttc atctagaact tctacatctg taggcttaac
     1681 atctcttata cttgtcattt tatacttacg tgtttcatta ccaactttaa agtacaccat
     1741 actacctttt ttggctgctt taagatttgt aaattgatag ttcggacggt caatgaaagt
     1801 gtgtcctgca attgaaatat tttgatcatc tagtgattcg ttttcttctg caaagcttac
     1861 acctctattt aattgttcag gtgttgctgg tcctggatat actggttctt taatatcagc
     1921 atctggaatt tcaatatagc ctgccacttt cgatttatct ttcggaattt gaggtttagc
     1981 ttgctgcttt ttatctttac tcgcctgttc ttttacattt ttatcatatt gttcaatctt
     2041 ttcatcttta tctttatcgt gaagataatt atcgatatgt ggtttagcaa acaaatatgc
     2101 tgccactagg ataagtacca caccagcgat tgtcattaat cgatttgtcc attttttcat
     2161 acgttaaggc tccttttata catttcacaa tgcctgttaa catttcatat agtataccat
     2221 taatttcaaa atgactcata gcaattcatt ttatactata aaatttacat gtatactttt
     2281 acgttagatt tcattacaca tatttgcatt caaataacga aacgctttta ataattacta
     2341 agggggaatt gatatgatta gatacgctaa aaaagaggat ttaaacgcta tattagcgat
     2401 atacaatgat gccattatca atactacagc tgtttatact tatgaaccac aaaccataga
     2461 cgaacgtgtc gcatggtttg aaacgaaaca acgtaagcat gagcctatct ttgtatttga
     2521 ggaaaatgga agtgtcttag ggtttgcgac gttcggttcg tttagacctt ggccagcata
     2581 cctatataca atcgaacatt ctatttatgt cgatgcttca gctagaggaa aaggtattgc
     2641 tagtcaatta ctacaccatt taattgttga agcaaaagct aaaggttatc gtgcgctagt
     2701 tgcaggcatt gatgcttcca acaaagcgag tattcagttg catcaaaagt ttgcttttaa
     2761 gcatgccggc acactgacca atgtaggttt taaattcaat caatggttag atttagcatt
     2821 ttacgaatta gatttacaag actagtaatg tttgaatcac ataatataaa caagacaacc
     2881 atgttaattc ccttaacata acaagccaac atataaaatt ttaaacttct ca
//
LOCUS       DAMZKC010000217         2898 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.220, whole genome shotgun sequence.
ACCESSION   DAMZKC010000217 DAMZKC010000000
VERSION     DAMZKC010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.220"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            190..300
                     /locus_tag="POB55_002464"
     CDS             190..300
                     /locus_tag="POB55_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001807442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDI6501403.1"
                     /translation="MVRLYKNGKPRNEIIREYDLTPSALGKWIKQHQNTG"
     gene            423..1097
                     /locus_tag="POB55_002465"
     CDS             423..1097
                     /locus_tag="POB55_002465"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDI6501404.1"
                     /translation="MEVIQKNAHQYSVPAMCKVLKIPRSTYYDSIKRKDNKITKDDSN
                     VERAVINIFNSNRKVFGARRKIARIMKKHNLVSVYTKAKYKNYPKETNEKRIKNHLNR
                     AFNREQPMETLVSDLTYVKVAGTWHYICLFIDLFNREIVGYSAGKNKDANLVSKAISR
                     INHNLEQINLFHTDRGKDFDNHLIDEVLESFKIKCSLSTKSCPYDNAVAEATMKAMRS
                     EFVKQM"
     gene            1101..1229
                     /locus_tag="POB55_002466"
                     /pseudo
     CDS             1101..1229
                     /locus_tag="POB55_002466"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1569..2825)
                     /locus_tag="POB55_002467"
     CDS             complement(1569..2825)
                     /locus_tag="POB55_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3578 domain-containing protein"
                     /protein_id="HDI6501405.1"
                     /translation="MIDNFKQVLKLIKEKRTNNVVKKSDWDKGDQYKTLVHDKLPKQL
                     KAHIKEDKYSVVGKVATGNYSKVPWISIYDENITKESKDGYYLVYLFHPEGEGIYLSL
                     NQGWSKISNMFPRDKNAAKQRALTLSSELNKYITSNEFNTGRFYYAENKDSSYDLKND
                     YPSGYSHGSIRIKYYDLNEGFTEEEMLYDLKKFLELFNELASKVTKTFYDGLVNRIDE
                     IQEDSEIEEIRIAQIDKTLKQVEAPKGVIPKYKKGVSKTTKNDSEIEKSNKENKLTGK
                     VGEKLALKYFNDLIDNKIDEDKKEQFRNILNDNPGSQHGHGYDLVAFDPTNTDEAIEK
                     FIEIKTSISSNIEEPFFMSLNEMFALKEYKKKYLILRIFNVSGEEPQFYFIDPYANYS
                     EFKDVDDLIDKVFNVEAIQYKVFGEK"
BASE COUNT         1032 a          421 c          392 g         1053 t
ORIGIN      
        1 gaatggaaat gatagtttaa taatgtacat tttttaaaaa cctagacata attttattga
       61 ggtgaacaat gaatcgtcta cacttaattg gacacattct atgagagtag attttgttaa
      121 tttaagaaag taggcgattt tattatgact agagaaagaa gatcatttag ttcaaatttt
      181 aagttataaa tggttagatt atataaaaat ggtaagccta ggaatgaaat tatacgcgag
      241 tatgatttaa caccttccgc gttagggaaa tggataaagc aacatcaaaa cacaggttga
      301 ttcaatcatc aagatagctt aacagatgaa gaaaaagagc tgattaaatt aggcaatgaa
      361 gttcaacata taaaaatgga gaacgatatt ttaaagcaag cagcgctgat cataggacga
      421 aaatagaagt cattcaaaag aatgcacatc aatattcagt tccggcaatg tgtaaagtcc
      481 taaaaatacc aagaagtacc tattatgatt ctataaaaag aaaagataat aaaatcacta
      541 aagatgattc aaacgtagaa cgtgctgtca taaatatttt taattctaat agaaaagtct
      601 ttggtgcaag acgaaagata gctcgtatca tgaaaaaaca taatctagtt tctgtttata
      661 cgaaagctaa atacaaaaat tatccaaaag aaactaatga aaaacgaatt aaaaatcatt
      721 tgaatcgcgc ttttaataga gaacaaccaa tggagacatt agtaagtgat ttgacatatg
      781 taaaagtcgc aggaacatgg cattacatat gtttatttat agatctattt aatagagaaa
      841 ttgttggtta cagtgcaggt aaaaataagg acgcaaattt agtatcaaaa gcaatcagca
      901 gaataaatca taatcttgaa caaatcaacc tgttccatac cgatagaggc aaagactttg
      961 ataatcattt gatagatgaa gtcctagaat cctttaaaat caaatgttca ttaagtacca
     1021 aaagttgtcc ttatgataac gcagttgcgg aagcaacgat gaaagcaatg agatctgaat
     1081 ttgtaaaaca aatgtaattt gaaaacttag aacagttaga aacagaatta tttgattatg
     1141 taaattggta taacaatttt agactacatt cttcattaca gtatttgacg ccagtggcat
     1201 ttaaaaatct acatatgaaa aatgtctaaa aaaatgttga cataccaggt tatagaatac
     1261 caattggaag tatttatgag tttcatcgaa gatgttaaac tgtccagctt aatttgacaa
     1321 tctaaatgta aaatcattaa taattaaaca aaacttaaaa tatttaactt tttgtgatta
     1381 ttccacatta tcttatctgc tctttatcac cataaaaata gaaaaaacaa gattcctaaa
     1441 gaatatagga atcttgtttc agactgtgga caaactgatt ttttatcagt tagcttattt
     1501 agaaagtttt atttaaatta cagtttctat attttattag atcactattt tagctcttgt
     1561 tcaagtaatc atttttcgcc aaaaacttta tattgaatag cttctacatt aaatacttta
     1621 tcaatgagat catctacatc tttaaattca gaataatttg catacggatc tataaaataa
     1681 aattgtggtt cttcaccaga aacattaaat attcttaata ttaaatattt cttcttatac
     1741 tctttcaaag caaacatttc atttagcgac ataaaaaatg gttcctcaat attagaagat
     1801 atagatgttt taatttcaat aaatttttct atagcttcat ctgtatttgt tggatcaaaa
     1861 gctactaaat catagccatg accgtgttga gagcctggat tatcatttaa aatattccta
     1921 aactgttctt tcttatcttc gtctatttta ttatcaatta gatcattaaa gtattttagc
     1981 gctaattttt ctccaacttt accggttaat ttattctctt tatttgattt ttcaatttct
     2041 gaatcatttt tagtagtctt tgatacacct tttttatatt ttggaattac tcctttaggt
     2101 gcttccactt gcttgagtgt cttatctatt tgtgctattc taatttcttc aatttcacta
     2161 tcttcctgta tttcgtctat tctattgact aagccatcat agaatgtttt tgtaactttt
     2221 gaagctaatt cattaaatag ttctaaaaat ttctttaaat catatagcat ttcttcttct
     2281 gtgaaccctt cattcaaatc ataatatttg attcttattg atccatgaga atatcctgat
     2341 gggtaatcat tttttaaatc ataagatgaa tctttatttt ctgcgtaata aaatcttcca
     2401 gtattaaatt catttgatgt aatatattta ttgagttcgg aagataaagt taatgctctt
     2461 tgttttgcag catttttatc ccgcggaaac atattactta tctttgacca tccttgattc
     2521 aaagataagt atatgccttc tccttctgga tgaaaaagat ataccaaata atatccatcc
     2581 tttgattctt ttgttatatt ctcatcatat attgaaatcc aaggaacttt actatagttc
     2641 ccagtagcaa ccttccctac aactgaatat ttatcttctt ttatatgcgc ttttaactgc
     2701 ttgggtaact tatcatggac taaagtttta tattgatcac ctttatccca atcagatttt
     2761 ttaactacat tattggtacg tttctcttta attaatttaa ggacctgttt aaagttgtct
     2821 atcattggaa attcgctcct attataaaat atattatgtc tcattttctt caatatgtac
     2881 ttatttatat tttaccgt
//
LOCUS       DAMZKC010000218         2898 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.221, whole genome shotgun sequence.
ACCESSION   DAMZKC010000218 DAMZKC010000000
VERSION     DAMZKC010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.221"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            57..>2898
                     /locus_tag="POB55_002468"
     CDS             57..>2898
                     /locus_tag="POB55_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047430389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding adhesin SdrG C-terminal
                     domain-containing protein"
                     /protein_id="HDI6501406.1"
                     /translation="MINRDNKKAITKKGMISNRLNKFSIRKYTVGTASILVGTTLIFG
                     LGNQEAKAAENTSTENAKQDDATTSDNKEVVSEAENNSTTENDSTNPIKKETNTDSQP
                     EAKEESTKSSTQQQQNNVTATTETKPQNIEKENVKPSTDKTATEDTSVILEEKKAPNN
                     TNNDVTTKPSTSEIQTKPTTPQESTNIENSQPQPTPSKVDNQVTDATNPKEPVNVSKE
                     ELKNNPEKLKELVRNDSNTDHSTKPVATAPTSVAPKRVNAKMRFAVAQPAAVASNNVN
                     DLIKVTKQTIKVGDGKDNVAAAHDGKDIEYDTEFTIDNKVKKGDTMTINYDKNVIPSD
                     LTDKNDPIDITDPSGEVIAKGTFDKATKQITYTFTDYVDKYEDIKSRLTLYSYIDKKT
                     VPNETSLNLTFATAGKETSQNVTVDYQDPMVHGDSNIQSIFTKLDEDKQTIEQQIYVN
                     PLKKSATNTKVDIAGSQVDDYGNIKLGNGSTIIDQNTEIKVYKVNSDQQLPQSNRIYD
                     FSQYEDVTSQFDNKKSFSNNVATLDFGDINSAYIIKVVSKYTPTSDGELDIAQGTSMR
                     TTDKYGYYNYAGYSNFIVTSNDSGGGDGTVKPEEKLYKIGDYVWEDVDKDGVQGTDSK
                     EKPMANVLVTLTYPDGTTKSVRTDAKGHYEFGGLKDGETYTVKFETPTGYLPTKVNGT
                     TDGEKDSNGSSVTVKINGKDDMSLDTGFYKEPKYNLGDYVWEDTNKDGIQDANEPGIK
                     DVKVTLKDSTGKVIGTTTTDASGKYKFTDLDNGNYTVEFETPAGYTPTVKNTTAEDKD
                     SNGLTTTGVIKDADNMTLDSGFYKTPKYSLGDYVWYDSNKDGKQDSTEKGIKDVTVTL
                     QNEKGEVIGTTKTDENGKYRFDNLDSGKYKVIFEKPAGLTQTVTNTTEDDKDADGGEV
                     DVTITDHDDFTLDNGYFEEDTSDSDSDSDSDSD"
BASE COUNT         1209 a          434 c          507 g          748 t
ORIGIN      
        1 tttatatgta aaaatattaa ttttttgcaa atattgatat tttaaaggag atttatatga
       61 ttaacaggga taataaaaag gcaataacaa aaaagggtat gatttcaaat cgcttaaaca
      121 aattttcgat tagaaagtat actgtaggaa ctgcatcgat tttagtaggt acgacattga
      181 tttttggtct agggaaccaa gaagctaaag ctgctgaaaa cactagtaca gaaaatgcaa
      241 aacaagatga tgcaacgact agtgataata aagaagtagt gtcagaagct gaaaataatt
      301 cgacaacaga aaatgattca acaaatccaa ttaagaaaga aacaaatact gattcacaac
      361 cagaagctaa agaagaatca actaaatcaa gtactcaaca acagcaaaat aacgttacag
      421 ctacaactga aactaagcct caaaacattg aaaaagaaaa tgttaaacct tcaactgata
      481 aaaccgcgac agaagataca tctgttattt tagaagagaa gaaagcacca aataatacaa
      541 ataacgatgt aactacaaaa ccatctacaa gtgaaattca aacaaaacca actacacctc
      601 aagaatctac aaatattgaa aattcacaac cgcaaccaac gccttcaaaa gtagacaatc
      661 aagttacaga tgcaactaat ccaaaagaac cagtaaatgt gtcaaaagaa gaacttaaaa
      721 ataatcctga gaaattaaaa gaattggtta gaaatgatag caatacagat cattcaacta
      781 aaccagttgc tacagctcca acaagtgttg caccaaaacg tgtaaacgca aaaatgcgct
      841 ttgcagttgc acaaccagca gcagttgctt caaacaatgt aaatgattta attaaagtga
      901 cgaagcaaac aatcaaagtt ggcgatggta aagataatgt ggcagcagcg catgacggta
      961 aagatattga atatgataca gagtttacaa ttgacaataa agtcaaaaaa ggcgatacaa
     1021 tgacgattaa ttatgataag aatgtaattc cttcggattt aacagataaa aatgatccta
     1081 tcgatattac tgatccatca ggagaggtca ttgctaaagg aacatttgat aaagcaacta
     1141 agcaaatcac atatacattt acagactatg tagataaata tgaagatata aaatcacgct
     1201 taactctata ttcgtatatt gataaaaaaa cagttccaaa tgagacaagt ttgaatttaa
     1261 catttgctac agcaggtaaa gaaacaagcc aaaatgtcac tgttgattat caagatccaa
     1321 tggtccatgg tgattcaaac attcaatcta tctttacaaa attagatgaa gataagcaaa
     1381 ctattgaaca acaaatttat gttaacccat tgaaaaaatc agcaaccaac actaaagttg
     1441 atatagctgg tagtcaagta gatgattatg gaaatattaa actaggaaat ggtagcacca
     1501 ttattgacca aaatacagaa ataaaggttt ataaagttaa ctctgatcaa caattgcctc
     1561 aaagtaatag aatctatgat tttagtcaat acgaagatgt aacaagtcaa tttgataata
     1621 aaaaatcatt tagtaataat gtagcaacat tggattttgg tgatattaat tcagcctata
     1681 ttatcaaagt tgttagtaaa tatacaccta catcagatgg cgaactagat attgcccaag
     1741 gtactagtat gagaacaact gataaatatg gttattataa ttatgcagga tattcaaact
     1801 tcatcgtaac ttctaatgac tctggtggtg gcgacggtac tgttaaacct gaagaaaagt
     1861 tatacaaaat tggtgactat gtatgggaag acgttgataa agacggtgtt caaggtacag
     1921 attcaaaaga aaaaccaatg gcaaacgttt tagttacatt aacttacccg gacggtacta
     1981 caaaatcagt aagaacagat gctaaaggtc attacgaatt cggtggtttg aaagacggag
     2041 aaacttatac agttaaattc gaaacgccaa ctggatatct tccaacaaaa gtaaatggaa
     2101 caactgatgg tgaaaaagac tcaaatggta gttctgtaac tgttaaaatt aatggtaaag
     2161 atgatatgtc tttagacact ggtttttata aagaacctaa atataatctt ggtgactatg
     2221 tatgggaaga tactaataaa gatggtatcc aagatgcaaa tgagccagga atcaaagatg
     2281 ttaaggttac attaaaagat agtactggaa aagttattgg tacaactact actgatgcct
     2341 cgggtaaata taaatttaca gatttagata atggtaatta tactgttgaa ttcgaaacac
     2401 cagcaggtta cacgccaacg gttaaaaaca ctacagctga agataaagat tccaatggtt
     2461 taacaacaac aggtgtcatt aaagatgcag ataatatgac attagacagt ggtttctata
     2521 aaacaccaaa atacagttta ggtgattatg tttggtacga cagtaataaa gatggcaaac
     2581 aagactcaac tgaaaaaggt attaaagatg tgacagttac attgcaaaac gaaaaaggcg
     2641 aagtaattgg aacaactaaa acagatgaaa atggtaaata tcgtttcgat aatttagata
     2701 gcggtaaata caaagttatt tttgaaaagc ctgctggctt aacacaaaca gttacaaata
     2761 caactgaaga tgataaagat gcagatggtg gcgaagttga cgtaacaatt acggatcatg
     2821 atgatttcac acttgataac ggatacttcg aagaagatac atcagacagt gattcagact
     2881 cagacagcga ttcagact
//
LOCUS       DAMZKC010000219         2893 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.222, whole genome shotgun sequence.
ACCESSION   DAMZKC010000219 DAMZKC010000000
VERSION     DAMZKC010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.222"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..1290
                     /locus_tag="POB55_002469"
     CDS             <1..1290
                     /locus_tag="POB55_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501407.1"
                     /translation="NSEDNMYLAQRIMYQVDDILKEDMLNEYYYLPKTLYNTLASPEF
                     DDLKRTDASQVDGQDDTSEDDDNESEKADSKSADSESKGGAYLEMELHEGQNSETLGN
                     DEAREGDATDDMTDMMTKKGKGSNDTLNREEGDAVGQSQAFQLDGVNKNVEIKWQIPE
                     IEPQYVLEYQESKQDVQYEIKDLIQIIKKTIEREQRDARFNLTKGRLQKDLINWFIDD
                     QYKLFYKKQDLSKSFDATFTLLIDASASMHDKMAETKKGVVLFHETLKALNIKHEILS
                     FSEDAFDSDDHAQPNIINEIINYDYSTFEKDGPRIMALEPQDDNRDGVAIRVASERLM
                     RRNQHQRFLIVFSDGEPSAFNYSQDGIIDTYEAVEMSRKFGIEVFNVFLSQDPITEDV
                     EQTIHNIYGQYAIFVEGVAHLPGHLSPLLKKLLLKSL"
     gene            1515..2858
                     /gene="brnQ"
                     /locus_tag="POB55_002470"
     CDS             1515..2858
                     /gene="brnQ"
                     /locus_tag="POB55_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDI6501408.1"
                     /translation="MNKNTWVIGFTLFAMFFGAGNLIFPPNLGLDSGQFFWPAILAFV
                     LTGIGLPLLGVIVGALDKEGYIGALNKISPKFSILFLIIIYLTIGPLFAIPRTASTSF
                     EMTITPIIHSNSSIALFIFTIIYFIVVLYICLNPSKLIDRIGSLLTPLLLITILAMII
                     KGYLDFSGNSAGKGNEALYHSNFSSFAEGFTQGYLTMDAIAAIAFSMIVVNAVKLTGI
                     TKTNQIFKQTLTAGLIAAVALIFIYISLGYIGNHMPVSDMTLDQLKSKDRNIGTYLLT
                     TMASTGFGSFGKYLLGIIVALACLTTACGLIVAVSEYFHRIVPKVSYKAFVLVFILMS
                     FIIANQGLNAVISMSIPVLSIVYPVAITVVLLILIAKFIPTKRISQQIPVIIVFILSI
                     FSVISKLGWLKINFIESLPLRAYSLEWFPVAIIATILGYLVGIFVKQDPIKYQQE"
BASE COUNT         1002 a          417 c          502 g          972 t
ORIGIN      
        1 aattctgaag ataatatgta tctggctcaa agaattatgt atcaagtaga tgatatttta
       61 aaagaagata tgctaaacga atattattac ttacctaaaa cactatataa tacattagct
      121 agtccagagt ttgatgattt aaagagaact gatgcaagtc aagtcgatgg gcaagatgat
      181 acttcagaag atgatgataa cgaaagtgaa aaagctgact ctaaatctgc agatagtgag
      241 tcaaaaggtg gcgcctattt agaaatggaa cttcacgaag gacaaaatag tgaaacattg
      301 ggtaatgatg aagcacgtga aggcgatgct acagatgata tgactgatat gatgaccaaa
      361 aagggcaaag gttcaaacga tacgctaaat cgtgaagaag gcgatgctgt tggtcaaagc
      421 caagcatttc aacttgatgg tgtgaacaaa aatgtagaaa taaaatggca aattcctgaa
      481 attgagccac aatatgtttt agaatatcaa gaatcgaaac aagacgtaca atatgaaatc
      541 aaagacttaa ttcaaattat aaagaaaacg attgaacgag aacaacgaga tgcacgtttt
      601 aatttaacaa aaggacgatt acaaaaggat ttaattaatt ggtttatcga cgatcaatat
      661 aaattgtttt acaaaaaaca agatttaagt aaatcatttg atgcgacgtt tacattgtta
      721 attgatgcgt cagcaagtat gcatgataaa atggctgaaa cgaagaaagg tgtagtgtta
      781 ttccacgaga cgttaaaagc attgaacatt aaacatgaaa ttttatcatt cagtgaggat
      841 gcctttgatt cagacgatca tgcacaacca aatatcatta atgaaattat taactatgat
      901 tactcaacct ttgaaaaaga tggcccacgt attatggcac ttgaacctca agatgataat
      961 cgtgatggtg ttgctattag agttgcaagt gaacgattaa tgcgtcgaaa tcaacatcaa
     1021 cgatttttaa tcgtattttc agacggcgaa ccgtctgcat ttaattatag tcaagatggt
     1081 attattgata cgtatgaagc tgtagaaatg tcacgtaaat ttggtattga agtatttaat
     1141 gtatttttga gtcaagatcc aataactgaa gatgttgaac aaacaattca taatatttat
     1201 ggtcaatatg ctattttcgt tgaaggtgtt gcgcatttac ctggacatct ctctccatta
     1261 ttaaaaaaat tactacttaa atctttataa ataagtttaa aatatgagtt tatatgaaac
     1321 tgtgaataaa agtcatcagt tactatttaa aaagtaaatt tgaagaattt tattttctgg
     1381 cataatttga tttaaacaac acatgtgttt tgtttctgct atgtacaaca cattccatat
     1441 agactcttta ttattttttg tgctaaaata tttacttgta aattatttcg tgttttaaaa
     1501 aaaggagtaa ctctatgaat aaaaatacat gggtcattgg gtttacgctc ttcgctatgt
     1561 tttttggcgc aggcaatcta atttttcctc ctaatctcgg attggatagt ggtcaatttt
     1621 tctggcccgc cattttagcg tttgttctaa ctgggattgg tttaccatta ttaggtgtga
     1681 ttgtaggtgc acttgataaa gaaggatata ttggcgcatt aaataaaatt tcacctaaat
     1741 tttcaatatt gttcttaatc atcatttatt tgactatagg accacttttc gcaataccta
     1801 gaactgcatc tacatctttt gaaatgacaa ttacaccaat tatacatagc aatagtagta
     1861 tcgctttatt tatatttacg attatctact tcatagtcgt tttgtatatt tgtttaaatc
     1921 catctaagtt aatcgatcgt attggttcat tattaacacc attattattg attactattt
     1981 tagcgatgat tattaaagga tacttagact ttagcggtaa tagtgctgga aagggcaatg
     2041 aagcactata tcattctaat ttttcaagtt ttgctgaagg ctttacacaa ggctatttaa
     2101 caatggatgc cattgcagca attgcttttt caatgattgt tgttaatgca gtaaaactaa
     2161 caggcattac taaaacaaat caaatattca aacaaacttt gactgctggt ttaattgcag
     2221 ccgtagcttt aattttcata tatatttcat taggttatat tggtaatcat atgccagtaa
     2281 gtgacatgac gttagatcaa ttgaaatcca aagatcgaaa cattgggaca tatttattaa
     2341 cgacaatggc ttcaacagga tttggttcat tcggaaaata tttattgggc atcattgtgg
     2401 ctctggcatg tctaactaca gcatgcgggc ttattgttgc agtttctgaa tatttccata
     2461 gaatcgtacc taaagtatca tacaaagcat ttgtattagt tttcatttta atgagtttta
     2521 ttattgctaa ccaaggttta aatgctgtta tctcaatgtc aattccggta ttaagcattg
     2581 tatacccagt agcaataact gttgtattat taattttaat tgccaaattc ataccgacaa
     2641 aacgcatttc acaacaaatt ccagttatta tcgtatttat attgtcgatt ttcagtgtta
     2701 ttagtaagtt aggttggctg aaaattaact ttatagaatc attgcctcta agagcgtatt
     2761 ctttagagtg gttcccagta gcaattattg caacgatatt aggctatcta gtcggcatat
     2821 ttgtaaaaca agatccaatt aaatatcaac aggaataacg aataatataa aagaggttgg
     2881 gacataaatc cct
//
LOCUS       DAMZKC010000220         2885 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.223, whole genome shotgun sequence.
ACCESSION   DAMZKC010000220 DAMZKC010000000
VERSION     DAMZKC010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.223"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            363..1403
                     /locus_tag="POB55_002471"
     CDS             363..1403
                     /locus_tag="POB55_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HDI6501409.1"
                     /translation="MQQVISDIMTFRGSHFELGVKTGKWLQQTPLLKNREKEWKKRVP
                     RFDIDVNETYQIFQTYAPQIWEELMGLQSILKMPTRQIILNFGHYRFTDLKESGCTVF
                     QGKDFMVRNYDYHPATYDGRYLLYQPTDSGLAQIGPVSRVTGRMDGMNESGLTMGYNF
                     MHRKKPANGFVCYMIGRLILENCRNVTEAIQLLKEIPHRSSFSYILMDKSLNHAIVEV
                     TPRSIDVRYDNICTNHFEILTHENRNYTKESKERLARTISQTNDNLDMTTAFKLFNNP
                     QYEIYSKLFKSWSGTIHTSMYQPETLTAYFTLGENAPPEIIDFKSWLDGQDLNITHFT
                     GNIDTDLTFANK"
     gene            complement(1460..2302)
                     /locus_tag="POB55_002472"
     CDS             complement(1460..2302)
                     /locus_tag="POB55_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HDI6501410.1"
                     /translation="MKKFFFIGLLVFVVFFTAATIIWFSYDKNKYGTKQYDKTFKDDA
                     FDNVSINLDSTELRIKRGNQFRVKYDGDNDILINIVDKTLKISDKRSKTRGYAIDMNP
                     FHENKKTLTIEMPDKMIKRLNLSSGAGSVRISDVDLENTSIQSINGEVVIKNSNLDAL
                     DSKTNNSSTYISKSNIKNSNIKVVIGTLQIDKSQIKQSIFLNDHGDIEFKNMPSKVDA
                     KASTKQGDIRFKYDSKPEDTILKLNPGTGDSVVKNKTFTNGKVGKSDNVLEFYTIDGN
                     IKVE"
     gene            complement(2299..2856)
                     /locus_tag="POB55_002473"
     CDS             complement(2299..2856)
                     /locus_tag="POB55_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001669025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HDI6501411.1"
                     /translation="MNKITYLNELETALDALPRHLRDQKMYEYERYFYEQELNGVDEG
                     EILKKLKDPQDVAAETKARSVIDYAESKPTFENISRAVAASLSLGILSIFVILIPVSI
                     VGLFVLALFLISLLLLFCPIILLASAISRGIVDSISNVFFAISYSGLGLVFIIVIFKI
                     LEYIYRLILKYLLWYIKTVKGSVRK"
BASE COUNT         1020 a          490 c          390 g          985 t
ORIGIN      
        1 tacgaaatat attcatcaaa ttatatagtg agcaagctaa tgattatttt caaaaatgcc
       61 gaggaacctc tgtttcaacg tgacatgacc ttatatgcta atgttaacaa tgactattat
      121 cgtgaatgct aacttaagat tgttatttat gataagatga ttttaagttt ttaacttaat
      181 atccccacca tctataaaaa tatgttttaa ctgattgata agtcttatac accgagaaat
      241 cacctcaaac aattttatat ttcatttagt aaaatataac tttatttaac atgacattca
      301 atatacatac gattactatt tatttacaat atattacaac taaaaagaaa ggggccgatg
      361 atatgcaaca agtgatatca gatattatga cttttagagg ttcacatttc gagttaggtg
      421 ttaaaactgg taaatggctt caacaaacac ctcttctaaa aaatcgagaa aaagaatgga
      481 aaaaacgtgt cccacgtttc gatatagatg taaatgaaac ttatcaaata ttccaaactt
      541 acgcacctca aatatgggaa gaattaatgg gactacaaag tatactcaaa atgccaacac
      601 gccaaattat tttaaacttt ggacattatc gttttaccga tttaaaagaa agtggttgca
      661 cggtctttca aggcaaagac ttcatggtta gaaattatga ttaccaccct gcaacttatg
      721 atggtcgcta cttactatat caacctacag atagcggttt agctcaaatt ggtcctgtat
      781 caagggtaac tggaagaatg gatggtatga atgagtcagg attaactatg ggctataact
      841 ttatgcaccg gaaaaaacca gcaaatggtt ttgtatgtta tatgattggt cgtctaattc
      901 ttgaaaattg ccgaaatgta acagaagcaa tacaattatt gaaagaaata ccacacagaa
      961 gctcatttag ctacatactg atggacaaat cattgaacca tgcaattgtt gaagtgacac
     1021 cgagatcaat agatgttcgt tatgataata tatgtacaaa tcattttgaa atattaacac
     1081 atgaaaatcg taattataca aaagaatcta aagaaagact ggcgcgtacc atttcacaaa
     1141 ctaatgataa tttagatatg actacagcat ttaagttatt caataatcct cagtatgaaa
     1201 tttacagtaa actattcaaa agctggtctg gcaccatcca tacatcaatg tatcaacctg
     1261 aaacattaac tgcatatttt acattgggag aaaatgcgcc accagaaata atagatttca
     1321 aatcatggtt agatggtcaa gaccttaata tcactcactt tactggcaat atagataccg
     1381 acttaacttt tgccaacaaa taaataacac cgatattaga gaaattatgc ttcctaatat
     1441 cggtgcttac atcctttatt tattcaactt tgatattacc atcaatcgta taaaattcta
     1501 aaacattgtc gcttttccca actttaccat tagtaaatgt tttattttta actacgctat
     1561 cacccgttcc cggatttagc tttagtatag tgtcttcagg tttactatca tacttaaaac
     1621 gaatatctcc ttgtttagta gaagcttttg catctacttt tgatggcatg tttttaaatt
     1681 caatgtcacc atgatcgttt aaaaatatgg attgtttaat ttgactcttg tcgatttgta
     1741 gcgtaccaat aacaacttta atattgctat ttttaatgtt acttttacta atataagtag
     1801 aactattatt tgtctttgaa tctagtgcgt caagatttga gtttttaata actacttcac
     1861 cgttaatgct ttgaatactt gtgttctcta aatcaacatc actgattcta acacttcctg
     1921 ctccagatga tagatttaaa cgtttaatca ttttatcagg catttcaatc gttaacgttt
     1981 tcttattctc atgaaaagga ttcatatcaa ttgcatatcc tcttgtctta gaccttttat
     2041 cactaatctt caacgtctta tctactatat taattaatat atcattgtca ccatcatatt
     2101 taactctaaa ttgattcccc cgttttatac gaagttctgt actatccaaa tttatagata
     2161 cattgtcaaa agcatcgtct ttgaatgttt tatcatattg tttagtacca tatttgtttt
     2221 tatcatagct gaaccaaata atggttgctg ctgtaaaaaa gacaacaaac actaaaagcc
     2281 caataaaaaa gaatttcttc attttctaac gcttcctttg acagttttaa tataccaaag
     2341 taaatatttt aagattaaac gataaatgta ttctaaaatc ttaaatatga caatgataaa
     2401 tactaatcct aatcctgaat atgatatggc aaaaaataca ttactaattg agtccacaat
     2461 tcctctggat attgctgatg ctaataaaat aattggacaa aacagcagca aaagtgatat
     2521 taaaaataat gctaatacaa ataatccaac tatagatact ggtataagga tgacaaaaat
     2581 agatagaatg cctaaactta atgaagcagc aacagctctt gaaatatttt caaatgttgg
     2641 tttagattca gcataatcaa taacacttct agcttttgtt tcagctgcaa catcttgtgg
     2701 gtcttttaac ttctttaaaa tttccccttc atcaacgcca ttaagttctt gctcataaaa
     2761 ataacgttca tattcataca ttttttgatc tcttaaatgc ctaggtagcg catcaagtgc
     2821 tgtttctaat tcatttaaat aagtaatctt gttcatatag gtcactcatc ccaataaaat
     2881 tattc
//
LOCUS       DAMZKC010000221         2864 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.224, whole genome shotgun sequence.
ACCESSION   DAMZKC010000221 DAMZKC010000000
VERSION     DAMZKC010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.224"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(267..412)
                     /locus_tag="POB55_002474"
                     /pseudo
     CDS             complement(267..412)
                     /locus_tag="POB55_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500662.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
     gene            complement(585..1187)
                     /locus_tag="POB55_002475"
     CDS             complement(585..1187)
                     /locus_tag="POB55_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDI6501412.1"
                     /translation="MNKISKALTWFIISFIIFHLILFIMWGEHQEYWYLYTGIMLIAG
                     ISYVFYQRDIESKRLLTSIGVGIITAIILIMLQLLFSLITSNLSYSSLIKELARTGVN
                     WKWQMLVTLLFVIPCHELYMRTVLQKELTHFSIPKWVAILITAICSSSLFIYLDNWWI
                     VTFIFVAQVILSLSYEYTRRIATTSVAQIVAIILLLIFNA"
     gene            complement(1202..1378)
                     /locus_tag="POB55_002476"
     CDS             complement(1202..1378)
                     /locus_tag="POB55_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkvS family protein"
                     /protein_id="HDI6501413.1"
                     /translation="MTVAEVGNIVEFMDGLRGRVEKINDNSVIVDLTIMENFNDLDLP
                     EKTVINHKRYKIVE"
     gene            1577..2563
                     /locus_tag="POB55_002477"
     CDS             1577..2563
                     /locus_tag="POB55_002477"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HDI6501414.1"
                     /translation="MKFISNNNITDPTLNLAMEEYVLKNLPAEESYFLFYINRPSIIV
                     GKNQNTIEEVNQTYIDAHNIDVVRRISGGGAVYHDTGNLNFSFITDDDGNSFHNFQKF
                     TEPIVQALQSLGVNAELTGRNDIQVGQAKISGNAMVKVKNRMFSHGTLMLNSDLDEVQ
                     NALKVNPAKIKSKGIKSVRKRVANIQEFLNDPLEIEEFKKIILKTIFGETEVEEYKLT
                     DEDWENIEKLSNDKYRTWEWNYGRNPKYNFEREEKFEKGFVQIKFDVKRGKIEHAKIF
                     GDFFGVGDVTDLENALVGCLHDFEHIEEALSEYDLYHYFGDIDRHELIRLMS"
     gene            complement(2644..2862)
                     /locus_tag="POB55_002478"
     CDS             complement(2644..2862)
                     /locus_tag="POB55_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HDI6501415.1"
                     /translation="MKYNTNVKHTTLEAFVTTVNDLGIELIINEALREVRKRQLIELI
                     DDALVNKDEAAFNQYTAEYKNLEAFLGE"
BASE COUNT         1080 a          426 c          422 g          936 t
ORIGIN      
        1 agtactacca tataatcata attgatttct accacagtat gattttcctt tcaaatctga
       61 catgtttagc aggcaatata cggaaaatac ttcaaaattt tgttacacat ctaataaaaa
      121 tagacaatta aataaaagat atgttactat aaaaatgtaa aaagacgaca tgcaggaaca
      181 tgtcgcctaa tgagcccgtt aaaaagacgg tgactaaatg agattttctt taaccatcat
      241 tctatgtcaa agttttgaaa tgatggttat tttttattgc ttaaatttat tattgctact
      301 actataccaa tgaaagtgac gataaacata ccgaaactta acattaaatg taatgcgttt
      361 gcaatagata tcacaatagg cttttccttt caaaagtgtt aagtaatgcc ataggcacca
      421 ccccccttta ctcagatagc caccatcaat tcaacttgcc cacaaattat tatactattt
      481 ttataaaata ttaccactat aatgaagcat gcctcgaatc ttcgtattgc tattcttaag
      541 caaaaaagct tttcttagaa cacatacgtc taagaaaagc ttatttaagc attaaaaatt
      601 aataacaata taatagctac aatttgtgca acggatgttg ttgctatacg tcgagtatac
      661 tcataactta atgataatat cacttgtgca acaaatataa atgttactat ccaccaatta
      721 tctaaataaa taaataatga actagaacaa attgcagtta ttaatatagc tacccatttc
      781 ggtattgaaa aatgtgttaa ttctttttgt aaaacagttc tcatatataa ctcgtgacat
      841 ggaatgacaa aaagtaaagt tactaacatt tgccacttcc aattgacacc tgttcttgct
      901 aattctttaa ttaatgaact ataacttaaa ttagaagtta taagtgagaa taaaagttga
      961 agcataatta aaataattgc tgtaataata ccaacaccaa ttgatgtaag caaccgctta
     1021 gattcaatat ctctttgata aaatacataa ctgataccag caattagcat tatacctgta
     1081 tataaatacc agtattcttg gtgttcgccc cacataataa ataatatgag atgaaatata
     1141 atgaaactta taataaacca agttaaagcc ttcgagattt tattcattat aacttacctt
     1201 cttattcaac aatcttatat cgtttatgat tgataacagt tttttccggt aaatcaaggt
     1261 cattaaaatt ttccataatt gttaagtcaa caataacaga gttatcgttg attttttcaa
     1321 cacgacctct taatccatcc ataaactcaa caatgttacc cacttctgca acagtcattt
     1381 taagtcctcc ttaaatcata actaaattat attgtactaa aattcactta acaaataaac
     1441 tattaacaat caaaaataat aaaatgtaac tttaagccga tatttagaat gatttgtgag
     1501 tatataattg gcattttgag caaagttaag gcataaataa gtagagaata tatatagata
     1561 taagggagag attgtaatga aattcattag taataataat attacagatc caactttaaa
     1621 cttagcaatg gaagaatatg ttttaaaaaa tttaccagca gaagaaagtt actttttatt
     1681 ttacataaat agaccatcta tcattgttgg aaagaatcaa aatacgatag aggaagtaaa
     1741 tcagacatat atagatgctc ataacatcga tgtagtaaga agaatttctg gtggtggcgc
     1801 tgtttatcat gatactggca atttaaactt tagtttcata acagatgatg atggtaacag
     1861 tttccacaat ttccaaaaat tcactgaacc aattgttcaa gcattacaat ctttaggcgt
     1921 caatgctgaa ttaactggtc gtaacgatat tcaagttgga caagcgaaaa tctcagggaa
     1981 tgccatggtt aaagttaaaa atagaatgtt tagtcatggt acgttgatgt taaatagtga
     2041 tttagacgaa gttcagaatg cactaaaagt taatccagct aagattaaat caaaaggtat
     2101 taaatctgtt cgtaaacgag tagcaaacat tcaagaattt ttaaatgacc ctttagaaat
     2161 tgaagaattt aaaaaaatca tcttgaaaac tatctttgga gaaactgaag tagaagaata
     2221 taaattaacg gatgaagatt gggaaaatat tgaaaagtta agtaacgaca aatatagaac
     2281 ttgggaatgg aattatggta gaaaccctaa atataacttt gaacgcgagg aaaaatttga
     2341 aaaaggattt gtacaaatta agtttgatgt taaacgaggt aaaatcgaac atgcaaaaat
     2401 tttcggtgat ttctttggtg tcggagatgt cactgatctt gaaaatgcat tagttggctg
     2461 tctacacgac tttgagcaca tagaagaagc actatcagaa tatgatttat atcactattt
     2521 cggtgacata gatagacatg aattaattag attaatgtca taaacgatta aaggactatt
     2581 cttacatgta tgagatgtta agaatagtcc ttttatgatt gcgttatgga attcgaatca
     2641 atgttattca ccgagaaatg cctccaaatt tttgtattct gccgtatatt gattaaatgc
     2701 tgcttcatct ttattgacga gtgcgtcatc tataagttct atgagctgtc gttttcttac
     2761 ctctcgaagt gcttcattga taattaattc aatacccaaa tcattgacgg ttgtgacaaa
     2821 cgcttctaaa gttgtatgtt taacattagt attgtatttc atag
//
LOCUS       DAMZKC010000222         2862 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.225, whole genome shotgun sequence.
ACCESSION   DAMZKC010000222 DAMZKC010000000
VERSION     DAMZKC010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.225"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..147
                     /locus_tag="POB55_002479"
     CDS             <1..147
                     /locus_tag="POB55_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HDI6501416.1"
                     /translation="SMERELFEIKETSITHSDGHTSISKTPKVTGKGQQYFVNKFLGE
                     KQTS"
     gene            163..360
                     /locus_tag="POB55_002480"
     CDS             163..360
                     /locus_tag="POB55_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501417.1"
                     /translation="MQAQNKKVIYYYYDEAGNRRPVNIQYNDGYDLMIDPRFIEMTLE
                     RHPHLKNNFYGLIDGKEFKLD"
     gene            391..531
                     /locus_tag="POB55_002481"
     CDS             391..531
                     /locus_tag="POB55_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501418.1"
                     /translation="MLQKFRIAKEKNKLKLKLLKHASYCLERSNNPELLRAVAELLKK
                     VN"
     gene            complement(546..1178)
                     /locus_tag="POB55_002482"
     CDS             complement(546..1178)
                     /locus_tag="POB55_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501419.1"
                     /translation="MYIDPLKNVRFSINNVISNVEISKSMAIKQSLKPKYQLDIINRN
                     NINLFSDFKVDFHLNNLIEMNFNLRNSFSSLTFQRNLFSEETIKSFKELYRFDDEIVL
                     QAQQTIRDFYINPTAISTLAEAINSTYPINEQSTYKRHDEFVKRIENDFPHPFKKLIR
                     WSNGIAAGADIQIFVTNYINENDLHIQNSLIVAIVCLLSFLSTYCSHSKK"
     gene            1237..1557
                     /locus_tag="POB55_002483"
     CDS             1237..1557
                     /locus_tag="POB55_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HDI6501420.1"
                     /translation="MPHILNVTVPIPETHVLITKDEYDELIGYSLDPVWNMSDLKKKL
                     KIASDETIKDRLLFHPRFEKELRAQGIVHYPDENFNRWRFNARKMNKFVDEHFNEIYK
                     ERIK"
     gene            1554..1715
                     /locus_tag="POB55_002484"
     CDS             1554..1715
                     /locus_tag="POB55_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1270 domain-containing protein"
                     /protein_id="HDI6501421.1"
                     /translation="MSNIYKSYLLAVLCFTVLAIVLMPLLYFTTAWSIAGFASIATFI
                     FYKEYFYEE"
     gene            1810..2136
                     /locus_tag="POB55_002485"
     CDS             1810..2136
                     /locus_tag="POB55_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2482 family protein"
                     /protein_id="HDI6501422.1"
                     /translation="MTKNYKDMTQEELRDLLAEKNGELFEVVNEINKETEFAVLLFST
                     VGVSNGDTTSSSHCALGDIVGLANLLNNENDYHDIANVIEMYKLKKLLGLADNKEDEN
                     DVLQNG"
     gene            2117..2377
                     /locus_tag="POB55_002486"
     CDS             2117..2377
                     /locus_tag="POB55_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1108 family protein"
                     /protein_id="HDI6501423.1"
                     /translation="MYYKTGDVCQKIINVDGFDFRLRVKKRAYSVEIVVLDHEGNSID
                     GILVSDENDLYTALDILKQSIYEWIENNTDEQDKLMNLVMKW"
     gene            2386..2649
                     /locus_tag="POB55_002487"
     CDS             2386..2649
                     /locus_tag="POB55_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001638663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501424.1"
                     /translation="MRDTERNILNIFKTLFDEYTLSNQRALLEIERNHHGYLSINFLH
                     YHDSYKTNNKLVQIHEINPDSHERIKNLIIEVLRGHRKIKKGA"
     gene            2658..>2862
                     /locus_tag="POB55_002488"
     CDS             2658..>2862
                     /locus_tag="POB55_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDI6501425.1"
                     /translation="MKINKLTISNFAGIKEVTFNFDGKDAKIYGNNATGKTTTATALQ
                     WLLFDKGLDGSTKSFNPVPLNEKN"
BASE COUNT         1112 a          410 c          509 g          831 t
ORIGIN      
        1 tcaatggaac gtgagttatt cgaaattaaa gaaacatcaa tcacacattc ggacggtcac
       61 acatcaatta gtaagacgcc aaaagtaaca ggcaaaggac aacaatactt tgttaataag
      121 tttttaggag aaaaacaaac atcttaatag gaggaacgaa caatgcaagc tcaaaacaaa
      181 aaagtcattt attactacta tgacgaagcc ggtaatagac gacccgttaa tattcaatac
      241 aacgatggct acgacttaat gatagacccg cgttttattg aaatgacgct tgaaagacat
      301 ccgcatttaa aaaataactt ttatggatta atagatggaa aagaatttaa gttagattaa
      361 atttttggaa atgcaaagga ggcataacaa atgttacaaa aatttagaat cgcgaaagaa
      421 aaaaataaat taaaactcaa attactaaag catgctagtt actgtttaga aagaagtaac
      481 aaccctgaat tgttgcgagc agttgcagag ttgttaaaga aggttaacta aattaggcct
      541 tattattact ttttagaatg tgaacaatag gtcgataaaa aacttaataa acaaactata
      601 gcaactatca atgaattttg aatatgtaaa tcgttctcgt ttatatagtt tgttacaaag
      661 atttgaatgt cagcacctgc tgcaatgcca ttagaccatc ttattaactt tttgaaagga
      721 tgtggaaaat cattttcgat acgtttgaca aattcatcgt gtctcttgta ggtactttgc
      781 tcatttattg gataggtcga attgatggct tcagccaaag tagagatagc agttggattg
      841 atataaaaat ctctaatggt ctgttgtgct tgaagtacaa tctcatcatc aaacctatag
      901 agttccttaa aagattttat cgtttcttca gaaaataaat ttctttgaaa tgttagagat
      961 gaaaaagaat tacgcaaatt aaaattcatt tcaattaagt tgtttagatg aaagtctact
     1021 ttgaagtcag aaaataaatt tatgttgttt ctattaatta tatctaattg gtacttaggt
     1081 tttaaagatt gtttaattgc catactttta gaaatttcaa cattactaat tacgttatta
     1141 atagaaaaac gaacattttt taaaggatca atatacacca atatcacctc ctttcactag
     1201 gagataacaa cattatacac gaaaggaaag atagaaatgc cacatatttt aaacgtaaca
     1261 gttccaatac ctgaaacaca tgtacttatc acaaaagatg aatatgatga gctaattggt
     1321 tattcattag accctgtatg gaacatgagt gacttaaaga agaaattaaa aattgcttct
     1381 gatgagacta tcaaggacag attactattt catcctagat ttgaaaaaga actaagagcg
     1441 caaggaattg tgcattaccc agatgagaat tttaatcgct ggagatttaa cgcaagaaag
     1501 atgaataaat tcgtcgatga gcatttcaat gaaatatata aggagagaat aaaatgagca
     1561 acatttataa aagctaccta ttagcagtac tgtgcttcac agtcttagcg attgtgctta
     1621 tgccattgct gtacttcact acagcatggt caattgcagg attcgcaagt atcgcaacat
     1681 tcatattcta taaggaatac ttttatgaag aatgaaaaaa ctgctacttg cgccaacaag
     1741 taacagtgac aaacgattaa caaaattaat tcattttcaa tataaaacga aaaacggagg
     1801 aagtcaacta tgactaaaaa ttataaagac atgacgcagg aagaattaag agatttattg
     1861 gctgaaaaga atggagaatt gtttgaagta gtgaatgaaa tcaataaaga aactgaattt
     1921 gccgttttac ttttttcaac tgtaggggtt agcaatggag atactacatc atcgtcacat
     1981 tgtgcgcttg gggatattgt aggtcttgct aatttattga ataacgaaaa tgattaccac
     2041 gatatcgcta atgttatcga aatgtataaa ttaaaaaaac ttttaggtct agctgacaac
     2101 aaggaggacg agaatgatgt attacaaaac gggtgacgta tgtcaaaaaa taattaatgt
     2161 agatggcttt gattttcgat taagagttaa gaaacgagca tatagcgtcg aaatagttgt
     2221 tttagatcat gaggggaatt caattgacgg gatactagtt tctgacgaga acgatctata
     2281 cacagcgtta gatattttga aacaaagtat ttatgaatgg attgaaaata acacagatga
     2341 acaggacaaa ctaatgaact tagtcatgaa atggtaggta taagcatgag agacacagaa
     2401 agaaatatat tgaatatttt taagacgtta ttcgacgaat atactttgtc aaaccaacga
     2461 gcattattgg aaattgaacg taatcatcac ggatacttat cgattaattt cctgcactat
     2521 cacgacagtt acaaaacaaa caataagctt gtgcagatac atgaaatcaa tccagacagc
     2581 catgaacgaa taaaaaattt aattatcgag gtgctaagag gtcatcggaa gattaaaaaa
     2641 ggagcatgag gaaagatatg aaaataaata agttaactat atcgaacttt gctggaatca
     2701 aagaagtaac atttaacttt gacggtaaag atgcaaaaat atacggcaat aatgcgactg
     2761 gtaagactac aacagcaacc gcattacaat ggctgctttt cgataaaggt ttggacggat
     2821 caaccaaatc atttaaccct gtacctttaa acgaaaaaaa cg
//
LOCUS       DAMZKC010000223         2773 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.226, whole genome shotgun sequence.
ACCESSION   DAMZKC010000223 DAMZKC010000000
VERSION     DAMZKC010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.226"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(172..744)
                     /gene="raiA"
                     /locus_tag="POB55_002489"
     CDS             complement(172..744)
                     /gene="raiA"
                     /locus_tag="POB55_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDI6501426.1"
                     /translation="MIRFEIHGDNLTITDAIRNYIEEKIGKLERYFNDVPNAVAHVKV
                     KTYSNSATKIEVTIPLKNVTLRAEERNDDLYAGIDLINNKLERQVRKYKTRINRKSRD
                     RGDQEVFVAELQEMQETQVDNDAYDDNEIEIIRSKEFSLKPMDSEEAVLQMNLLGHDF
                     FVFTDRETDGTSIVYRRKDGKYGLIQTSEQ"
     gene            complement(805..1479)
                     /locus_tag="POB55_002490"
     CDS             complement(805..1479)
                     /locus_tag="POB55_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HDI6501427.1"
                     /translation="MNNCLSCGAKLYENITIYNLFKKPNRLCDRCKENWDNIKLDIKA
                     RRCSRCLKHLNQDEAYCLDCKFLSAHFNLMEQLYCQFQYDGLMKEMIHQYKFLKDYYL
                     CELLAHLIEIPQTSYDYIVPIPSSPAHDLSRTFNPVEAVLKAKGIRFDKILKMSNRPK
                     QSHLTKKERLADENPFIIDTELDLNGKEILLVDDIYTTGLTIHRAGCKLYAKNIRKFK
                     VFAFAR"
     gene            complement(1472..2554)
                     /locus_tag="POB55_002491"
     CDS             complement(1472..2554)
                     /locus_tag="POB55_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDI6501428.1"
                     /translation="MDNVTRYKITESSQSSSQAYYHLSFELSEQQSYASEHIVRAIRK
                     RQTILLYAVTGAGKTEMMFQGIQYARIQGDNIAIVSPRVDVVVEISKRIKDAFLNEDI
                     DILHQQSRQQFEGHFVVCTVHQLYRFKQHFDTIFIDEVDAFPLSMDKNLQQALKSSSK
                     VEHATIYMTATPPKQLLSEIPHENIIKLPARFHKKSLPVPKYRYFKLNNNKIQKMLYR
                     ILQDQINNQRYTLVFFNNIETMIKTFSVYKQKITKLTYVHSEDVFRFEKVEQLRNGHF
                     DVIFTTTILERGFTMANLDVVVIDAHQYTQEALIQIAGRVGRKLECPTGKVLFFHEGV
                     SMNMIQAKKEIQRMNKLALKRGWIDE"
BASE COUNT          855 a          483 c          388 g         1047 t
ORIGIN      
        1 agactgacat actattatta acttaacatt caaatatata catccattaa cattagcata
       61 gtcactatgt ttcattcaac aaattacatt atcgaactat gaaatagtca taatttgctt
      121 ttggagtata aaaaagcact tgtgcaaaaa cacaagtgct ttaaacttaa tttattgttc
      181 actagtttga atcaagccat atttaccgtc tttacggcgg taaacgatac ttgttccatc
      241 agtttctctg tctgtgaata caaagaagtc atgacctaat agattcattt gtaataccgc
      301 ttcttctgaa tccattggtt ttaagctgaa ttcttttgaa cgaataattt ctatctcgtt
      361 atcatcgtaa gcgtcattat caacttgtgt ttcttgcatt tcttgtaatt cggcaacaaa
      421 cacttcttga tctcctcgat cacggctctt acgattaata cgtgttttat attttcgaac
      481 ttgtctttca agtttattat taattaaatc aatacctgcg tataaatcat cgtttcgctc
      541 ttcagctctt aacgtaacat ttttcaatgg aattgttact tcaattttag tagctgaatt
      601 tgaataagtt ttaactttaa catgcgccac tgcatttggt acgtcattaa aataacgttc
      661 caacttacca attttttcct caatatagtt gcgaatagca tctgtgatag tgaggttatc
      721 tccatgaatt tcaaatctaa tcatagtaat ctctccttaa acctctttat tggtaactct
      781 ttattatatt ttaacatttt tacgctatcg tgcaaacgca aacactttga attttctgat
      841 atttttagca tataatttac accctgcacg atgaattgtt aatccagttg tataaatatc
      901 gtcaacgagt aatatttcct tgccatttaa atctaattcc gtatcaataa taaatggatt
      961 ttcatctgcc agacgctctt tcttagttaa atgagactgt tttggtctat ttgacatctt
     1021 taaaatctta tcaaagcgaa tccctttagc ttttagtact gcttctaccg ggttaaatgt
     1081 tctagataaa tcatgtgccg gcgaagaagg aattggcaca atatagtcat aagatgtttg
     1141 tggtatttca atcaaatgtg ccaataattc acataaataa tagtctttca aaaatttata
     1201 ctgatgtatc atctctttca ttaaaccgtc atattgaaat tgacaatata attgttccat
     1261 taaattaaag tgtgccgata gaaacttgca gtctaaacaa tacgcttcat cttgatttaa
     1321 gtgttttaag caccttgaac atcgccttgc tttaatatca agtttaatat tgtcccaatt
     1381 ctctttgcat ctgtcacata atctattagg tttcttgaac aaattataaa tggttatatt
     1441 ttcatataac ttagcaccac aactcaaaca attattcatc aatccaacct ctttttaatg
     1501 ctaatttgtt catcctttga atctcttttt tagcttgaat catattcata cttactcctt
     1561 catgaaaaaa caatactttt ccagtaggac attctaattt tcgtccaaca cgtccagcaa
     1621 tttgtattaa agcctcttga gtatattgat gtgcatcgat aacaacaaca tccaaatttg
     1681 ccattgtaaa tccacgttct aatattgtcg tagtaaaaat gacatcgaaa tgtccattcc
     1741 ttaattgttc aactttttca aagcgaaaaa catcctcgct atggacgtat gttaatttag
     1801 taattttctg cttataaacc gaaaatgttt taatcattgt ttctatattg ttaaaaaaca
     1861 ccagtgtata acgttgatta ttaatttgat cttgtaaaat tcggtataac attttctgaa
     1921 tcttattatt attaagtttg aaataacgat atttaggaac tggaagtgat tttttatgaa
     1981 agcgagctgg caatttaatt atattttcgt ggggaatctc tgacagaagt tgtttcggtg
     2041 gtgttgctgt catataaatt gttgcatgtt caactttaga agatgacttc aatgcttgtt
     2101 gtagattttt atccattgat aaaggaaagg catcgacttc atcaataaaa atagtatcaa
     2161 agtgctgttt gaatcggtaa agttgatgca ctgtgcatac aacaaaatgc ccttcaaatt
     2221 gttgtcttga ttgctggtgt agtatgtcta tatcttcatt aagaaatgcg tctttaatac
     2281 gtttactaat ttctacaaca acatctacac gtggtgacac aatagctata ttatctccct
     2341 gtattcttgc atattgaatg ccttgaaaca tcatttctgt cttacctgca cctgttacgg
     2401 catataacaa aatcgtttgt ctctttctaa tggctcgaac aatatgttct gaggcataag
     2461 actgctgttc cgacaattca aatgagagat gataatatgc ttgtgatgaa ctttgcgaac
     2521 tctctgttat tttatatctt gttacattat ccattcgacc cagtgatata caatttctgc
     2581 aatatacaat tttcttggaa tgtactgagg aatcatactg ataaaaatgc tttggatttg
     2641 tactttcaca ttgaacacaa taatagtttg aattttgttt gatgacacct gttacttctt
     2701 ttttaatttt ttcattagta agtgaagtat gaggatgaat taatttcccg taatgtttaa
     2761 tgcttaaaac ctc
//
LOCUS       DAMZKC010000224         2749 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.227, whole genome shotgun sequence.
ACCESSION   DAMZKC010000224 DAMZKC010000000
VERSION     DAMZKC010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.227"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            323..1624
                     /locus_tag="POB55_002492"
     CDS             323..1624
                     /locus_tag="POB55_002492"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HDI6501429.1"
                     /translation="MRMIDIIEKKRDGHTLTTEEINFFIDGYVKGDIPDYQASSLAMA
                     IYFQDMNDDERAALTMAMVNSGDMIDLSDIKGVKVDKHSTGGVGDTTTLVLAPLVAAV
                     DVPVAKMSGRGLGHTGGTIDKLEAIDGFHVEIDEATFVKLVNENKVAVVGQSGNLTPA
                     DKKLYALRDVTGTVNSIPLIASSIMSKKIAAGADAIVLDVKTGSGAFMKTLEDAEALA
                     HAMVRIGNNVGRNTMAIISDMNQPLGRAIGNALELQEAIDTLKGQGPKDLTELVLTLG
                     SQMVVLANKAETLEEARALLIEAINSGAALEKFKTFIKNQGGDETVIDHPERLPQAQY
                     QIEYKAKKSGYVTELVSNDIGVASMMLGAGRLTKEDDIDLAVGIVLNKKIGDKVEEGE
                     SLLTIHSNRQDVDDVVKKLDSSITIADHVVSPTLIHKIIIE"
     gene            1638..1988
                     /locus_tag="POB55_002493"
     CDS             1638..1988
                     /locus_tag="POB55_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501430.1"
                     /translation="MNILRYLLGIGFSVIGVLHFTRERQFRNIIPKCFPLRKTAVLVT
                     GIFEILFGLALLIKRPSQCLKNTINLFLLAVFPANIYMAVKKLPLGDKQLPTWQLYLR
                     LPMQFVLMGLIKKL"
     gene            complement(2140..2610)
                     /locus_tag="POB55_002494"
     CDS             complement(2140..2610)
                     /locus_tag="POB55_002494"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HDI6501431.1"
                     /translation="MTKMNVESFNLDHTKVVAPFIRLAGTMEGLNGDVIHKYDIRFKQ
                     PNKEHMDMPGLHSLEHLMAENIRNHSDKVVDLSPMGCQTGFYVSFINHDNYDDVLNIV
                     EATLNDVLNATEVPACNEVQCGWAASHSLEGAKTIAQAFLDKRNEWHDVFGTGK"
BASE COUNT          941 a          397 c          522 g          889 t
ORIGIN      
        1 gttcaaatta tgcaaggttt agaagcagat tcagattact aattttataa aaaaatatgt
       61 aagcatggga catcgtaacc atatataagt atgtcgatat cagctttaag acatcataaa
      121 tgccatgatg attctaagtt aatttaatag ggactttaac gttataacca atctatttaa
      181 gaataataac atcgcattga aattgtgata ttaaaaatga aatcgatagc ttgctataat
      241 aattttcgct tgtgctttta aaaatggact agagaaagcg cagatgcttt gtcctaagct
      301 ttttaagaaa aggatgataa aaatgagaat gatagacatt attgagaaaa agcgtgacgg
      361 tcatacacta acaacggaag aaattaattt ctttattgac ggctatgtta aaggggatat
      421 tcctgattac caagcatcaa gtttagcaat ggcgatttat ttccaagata tgaatgatga
      481 tgagcgtgca gcattaacga tggctatggt taattctggt gatatgatag atttgagtga
      541 tattaaaggt gtcaaagtag ataagcactc aacaggtggt gtaggagata caactacatt
      601 ggttttagca ccattagtag cagctgtaga tgttcctgtt gcaaaaatga gtgggcgtgg
      661 attaggtcat acaggtggta cgattgataa attagaagca attgatggtt ttcatgttga
      721 aatagatgaa gcaacatttg tgaaattggt taatgaaaat aaagtggcag ttgtaggaca
      781 atcaggaaat ttaactcctg cagacaaaaa attatatgcc ttaagggatg ttactggtac
      841 tgtcaattca ataccattaa ttgcctcttc aattatgagt aaaaagattg ctgctggtgc
      901 agatgcaatt gtattagatg taaaaactgg tagcggtgca tttatgaaaa cattagaaga
      961 tgctgaagca ttagcgcacg caatggtacg tattggtaat aatgtgggac gtaatacgat
     1021 ggcgattatt tctgatatga atcagccact tggacgtgcg attggcaatg cgcttgagtt
     1081 acaagaagca attgatacgt tgaaaggaca aggtccgaaa gatttaacag aacttgtatt
     1141 aacattaggt tctcaaatgg ttgtacttgc aaacaaagct gaaacgcttg aagaagcgag
     1201 agcgttgcta attgaggcaa ttaattcagg tgcagcatta gaaaaattca aaacatttat
     1261 taaaaaccaa ggtggcgacg aaactgttat tgaccatcca gagcgtttgc cacaagctca
     1321 atatcaaatt gaatataaag ctaaaaaatc aggttatgtg actgaattag tctctaacga
     1381 tataggtgtc gcttcgatga tgttaggagc gggacgttta acaaaagagg atgatattga
     1441 tttagcggtt ggtattgttt taaataaaaa aattggtgat aaagtagaag aaggagaatc
     1501 attattaacg attcacagta atcgccaaga tgtagacgat gttgttaaaa aattagattc
     1561 aagcataaca attgctgatc atgttgtatc gcctacatta attcataaaa tcattataga
     1621 ataggagttg aagctgattg aacatactaa gatatttatt aggaataggt tttagtgtaa
     1681 taggtgtatt gcattttaca cgagaacgac aatttagaaa tatcataccg aaatgtttcc
     1741 cacttcgaaa aacagcggtt cttgttacag gaatctttga gatattattt ggacttgcac
     1801 ttttaattaa aagaccatct caatgcttga aaaatacaat caatctattc ttgctagctg
     1861 tattcccagc taatatctat atggcagtta aaaagttacc tttaggagat aaacaattgc
     1921 caacatggca actatattta aggttaccta tgcagttcgt gttaatggga ctaattaaga
     1981 aattatagtg gttctcaaat aaatcggaca aatgttaaag attttacttt ttcatttgtt
     2041 cgattttttg actttgaagc ataataaaaa gcagttggac aatggcgatt gtaacatcat
     2101 tgttcgactg cttttctgat taacttgatt gactaagatt tattttcctg taccgaaaac
     2161 atcatgccat tcgtttcgtt tgtctagaaa tgcttgagcg atagttttag caccttctaa
     2221 tgaatgactt gctgcccagc cacattgtac ttcattacaa gcaggcactt cagtagcatt
     2281 tagcacatca tttaaagttg cttcaacaat atttaataca tcatcataat tatcatgatt
     2341 aataaatgat acatagaaac cagtttggca acccatagga cttaaatcaa caactttgtc
     2401 actatgattt ctaatatttt cagccattaa atgttctaat gaatgtagtc cgggcatatc
     2461 catatgttct ttgtttggtt gtttgaaacg aatgtcgtat ttgtgaatga catctccgtt
     2521 taatccttcc atcgttcccg ctaatctaat aaatggggca accactttag tatgatctaa
     2581 attgaaactt tcaacattca tttttgtcat tttgaatttc ctcctattaa ctactcaaaa
     2641 ctataaaacg cttatgattt gttgcattat aaaacttaag tttgttgtaa gttgtgtttc
     2701 taacaatact taaattgtaa caatagggct tatgttttac aatttgaaa
//
LOCUS       DAMZKC010000225         2746 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.228, whole genome shotgun sequence.
ACCESSION   DAMZKC010000225 DAMZKC010000000
VERSION     DAMZKC010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.228"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            128..592
                     /gene="accB"
                     /locus_tag="POB55_002495"
     CDS             128..592
                     /gene="accB"
                     /locus_tag="POB55_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HDI6501432.1"
                     /translation="MNFKEIKELIEILDKSTLTEINIEDTKGKVTLKKEKETEIITPQ
                     ISQMPVEAAAMPMPQVQSTDSNKTEAPKPTSDNHKTINAPMVGTFYKSPSPDEEAYVQ
                     VGDTVSNETTVCILEAMKLFNEIQAEISGEIVEILVEDGQMVEYGQPLFKVK"
     gene            592..1947
                     /gene="accC"
                     /locus_tag="POB55_002496"
     CDS             592..1947
                     /gene="accC"
                     /locus_tag="POB55_002496"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDI6501433.1"
                     /translation="MKKVLIANRGEIAVRIIRACRDLGIQTVAIYSEGDKDALHTQIA
                     DEAYCVGPTLSKDSYLNIPNILSIATSTGCDGVHPGYGFLAENADFAELCEACQLKFI
                     GPSYQSIQKMGIKDVAKAEMIKANVPVVPGSDGLMKDVSEAKKIAKKIGYPVIIKATA
                     GGGGKGIRVARDEKELETGFRMTEQEAQTAFGNGGLYMEKFIENFRHIEIQIVGDSYG
                     NVIHLGERDCTIQRRMQKLVEEAPSPILDDETRREMGNAAVRAAKAVNYENAGTIEFI
                     YDLNDNKFYFMEMNTRIQVEHPVTEMVTGIDLVKLQLQVAMGDVLPYKQEDIKLTGHA
                     IEFRINAENPYKNFMPSPGKIEQYLAPGGYGVRIESACYTNYTIPPYYDSMVAKLIIH
                     EPTRDEAIMAGIRALSEFVVLGIDTTIPFHIKLLNNDIFRSGKFNTNFLEQNSIMNDE
                     G"
     gene            1962..2324
                     /locus_tag="POB55_002497"
     CDS             1962..2324
                     /locus_tag="POB55_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDI6501434.1"
                     /translation="MVKVTDYSNSKLGKVEIAPEVLSVIASIATSEVEGITGHFAELK
                     ETNLEKVSRKNLSRDLKIESKEDGIYIDVYCALKHGVNISKTANKIQTSIFNSISNMT
                     AIEPKQINIHITQIVIEK"
     gene            2384..>2746
                     /gene="nusB"
                     /locus_tag="POB55_002498"
     CDS             2384..>2746
                     /gene="nusB"
                     /locus_tag="POB55_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HDI6501435.1"
                     /translation="MSRKESRVQAFQTLFQLEMKDSDLTINEAISFIKDDNPDLDFEF
                     IHWLVSGVKDHEPVLDETISPYLKDWTIARLLKTDRIILRMATYEILHSDTPAKVVMN
                     EAVELTKQFSDDDHYKFIN"
BASE COUNT         1016 a          399 c          534 g          797 t
ORIGIN      
        1 atgtgttttt attatatctt tgggatagac ttataatgtc gccagcaata tgaacatgct
       61 tgaattgaag agttgtctca agtaaaatag acgggtagat gaaaacaaac tgaaggagtc
      121 agtaataatg aactttaaag aaatcaaaga attaattgaa attctggata aatcaacttt
      181 aacggaaatc aatattgaag atactaaagg caaagtgacg cttaagaaag aaaaagaaac
      241 tgagattatc acgccacaaa tctcacaaat gccagttgaa gctgcggcaa tgcctatgcc
      301 tcaagtacaa tcaactgata gcaataaaac tgaagctcca aagccaactt cagataatca
      361 caaaacaatt aatgcaccta tggtaggtac attttacaaa tcgccatctc cagacgaaga
      421 agcatatgtg caagttgggg acactgtttc aaatgaaaca acagtgtgta ttttagaggc
      481 aatgaaacta tttaatgaaa ttcaagcaga aatttcaggt gaaattgttg aaatcttagt
      541 agaagacgga caaatggtag agtatggcca accgttattt aaggtgaaat aatgaaaaag
      601 gttttaattg caaaccgcgg tgaaatcgca gttaggatta ttcgcgcttg tcgtgattta
      661 ggcatccaaa ctgttgcaat ctattctgaa ggggataaag atgcgctaca tactcaaatt
      721 gctgatgaag catattgcgt aggtcccact ttgtctaaag attcatattt aaatattccg
      781 aacatcttat ctattgcaac ttctacaggt tgtgatggcg ttcatccggg ttatggcttt
      841 ttagctgaaa atgctgactt tgcagaatta tgcgaagcat gccaattgaa gttcattgga
      901 ccaagttatc aatctatcca aaaaatgggt atcaaagatg ttgctaaggc agaaatgatc
      961 aaagccaatg ttccagttgt tcctggtagt gatggtttaa tgaaagacgt ctcagaagct
     1021 aagaaaatcg ctaaaaaaat tggctatccg gtcatcatta aagctactgc tggtggtggc
     1081 ggaaaaggta ttcgtgttgc tcgtgatgaa aaagaacttg aaactggctt ccgaatgaca
     1141 gaacaagaag ctcaaactgc atttggtaat ggtggacttt atatggagaa attcatcgaa
     1201 aacttccgcc atattgaaat ccaaattgtt ggggacagct atggtaatgt aattcattta
     1261 ggagaacgtg attgtacaat tcaaagacgt atgcagaaat tagtggaaga agcaccttcc
     1321 ccaattttag atgatgaaac acgtcgtgaa atgggaaatg ccgcagtgcg tgcagcgaaa
     1381 gctgtaaatt atgaaaatgc gggaacaatt gagtttatat atgatttaaa tgataataaa
     1441 ttttatttta tggaaatgaa tacacgtatt caagtagaac atcctgtaac tgaaatggta
     1501 acaggaattg atttagttaa attacaatta caggttgcta tgggtgacgt gttaccgtat
     1561 aaacaagaag atattaaatt aacaggacac gcaattgaat ttagaattaa tgctgaaaat
     1621 ccttacaaga actttatgcc atcaccaggt aaaattgagc aatatcttgc accaggtgga
     1681 tatggtgttc gaatagagtc agcatgttat actaattata cgataccgcc atattatgat
     1741 tcgatggtag cgaaattaat catacatgaa ccgacacgag atgaagcgat tatggctggc
     1801 attcgtgcat taagtgaatt tgtggttctt ggtattgata caactattcc attccatatt
     1861 aaattattga ataacgatat atttagaagc ggtaaattta atacaaactt tttagagcaa
     1921 aatagcatta tgaatgatga aggttaatag gaggtcaatc catggtcaaa gtaactgatt
     1981 attcaaattc aaaattaggt aaagtagaaa tagcgccaga agtgctatct gttattgcaa
     2041 gtatagctac ttcggaagtc gaaggcatca ctggccattt tgctgaatta aaagaaacaa
     2101 atttagaaaa agttagtcgt aaaaatttaa gccgtgattt aaaaatcgag agtaaagaag
     2161 atggcatata tatagatgta tattgtgcat taaaacatgg tgttaatatt tcaaaaactg
     2221 caaacaaaat tcaaacgtca atttttaatt caatttctaa tatgacagcg atagaaccta
     2281 agcaaattaa tattcacatt acacaaatcg ttattgaaaa gtaatgtcat acctaattca
     2341 gtaattaaat aaagaaaaat acaaacgttt gaaggagtta aaaatgagtc gtaaagaatc
     2401 ccgagtgcaa gcttttcaaa ctttatttca attagaaatg aaggacagtg atttaacgat
     2461 aaatgaagcg ataagcttta ttaaagacga taatccagat ttagacttcg aatttattca
     2521 ttggctagtt tctggcgtta aagatcacga acctgtatta gacgagacaa ttagtcctta
     2581 tttaaaagat tggactattg cacgtttatt aaaaacggat cgtattattt taagaatggc
     2641 aacatatgaa atattacaca gtgatacacc tgctaaagtc gtaatgaatg aagcagttga
     2701 attaacaaaa caatttagtg atgatgatca ttataaattt ataaat
//
LOCUS       DAMZKC010000226         2742 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.229, whole genome shotgun sequence.
ACCESSION   DAMZKC010000226 DAMZKC010000000
VERSION     DAMZKC010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.229"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..885
                     /gene="lukS-PV"
                     /locus_tag="POB55_002499"
     CDS             <1..885
                     /gene="lukS-PV"
                     /locus_tag="POB55_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Panton-Valentine bi-component leukocidin subunit
                     S"
                     /protein_id="HDI6501436.1"
                     /translation="IATSFHESKADNNIENIGDGAEVVKRTEDTSSDKWGVTQNIQFD
                     FVKDKKYNKDALILKMQGFINSKTTYYNYKNTDHIKAMRWPFQYNIGLKTNDPNVDLI
                     NYLPKNKIDSVNVSQTLGYNIGGNFNSGPSTGGNGSFNYSKTISYNQQNYISEVERQN
                     SKSVQWGIKANSFITSLGKMSGHDPNLFVGYKPYSQNPRDYFVPDNELPPLVHSGFNP
                     SFIATVSHEKGSGDTSEFEITYGRNMDVTHATRRTTHYGNSYLEGSRIHNAFVNRNYT
                     VKYEVNWKTHEIKVKGHN"
     gene            881..1864
                     /gene="lukF-PV"
                     /locus_tag="POB55_002500"
     CDS             881..1864
                     /gene="lukF-PV"
                     /locus_tag="POB55_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Panton-Valentine bi-component leukocidin subunit
                     F"
                     /protein_id="HDI6501437.1"
                     /translation="MDMKKIVKSSVVTSIALLLLSNTVDAAQHITPVSEKKVDDKITL
                     YKTTATSDSDKLKISQILTFNFIKDKSYDKDTLILKAAGNIYSGYTKPNPKDTISSQF
                     YWGSKYNISINSDSNDSVNVVDYAPKNQNEEFQVQQTVGYSYGGDINISNGLSGGGNG
                     SKSFSETINYKQESYRTSLDKRTNFKKIGWDVEAHKIMNNGWGPYGRDSYHSTYGNEM
                     FLGSRQSNLNAGQNFLEYHKMPVLSRGNFNPEFIGVLSRKQNAAKKSKITVTYQREMD
                     RYTNFWNQLHWIGNNYKDENRATHTSIYEVDWENHTVKLIDTQSKEKNPMS"
     gene            2113..2298
                     /locus_tag="POB55_002501"
     CDS             2113..2298
                     /locus_tag="POB55_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501438.1"
                     /translation="MFILNTQAHARLDQMAQQFEVVCNGLNENEGQAIQTMDQSASLI
                     RSNLIQVKEQLEKLAVY"
     gene            2389..>2742
                     /locus_tag="POB55_002502"
     CDS             2389..>2742
                     /locus_tag="POB55_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 family protein"
                     /protein_id="HDI6501439.1"
                     /translation="MFKRTKLILIATILLSGCSTTNNESNNETKSVPEEMEASKYVGQ
                     GFQPPAEKDAVEFAKKHKDKIAKRGEQFFMDNFGLKVKATNVVGSGDGVEVFVHCDDH
                     DIVFNASIPFDKSIID"
BASE COUNT         1086 a          394 c          453 g          809 t
ORIGIN      
        1 attgctactt cgtttcatga atctaaagct gataacaata ttgagaatat tggtgatggc
       61 gctgaggtag tcaaaagaac agaagataca agtagcgata agtggggggt cacacaaaat
      121 attcagtttg attttgttaa agataaaaag tataacaaag acgctttgat tttaaaaatg
      181 caaggtttta tcaattcaaa gactacttat tacaattaca aaaacacaga tcatataaaa
      241 gcaatgaggt ggcctttcca atacaatatt ggtctcaaaa caaatgaccc caatgtagat
      301 ttaataaatt atctacctaa aaataaaata gattcagtaa atgttagtca aacattaggt
      361 tataacatag gtggtaattt taatagtggt ccatcaacag gaggtaatgg ttcatttaat
      421 tattcaaaaa caattagtta taatcaacaa aactatatca gtgaagtaga acgtcaaaat
      481 tcaaaaagtg ttcaatgggg aataaaagct aattcattta tcacatcatt aggtaaaatg
      541 tctggacatg atccaaattt atttgttgga tataaaccat atagtcaaaa tccgagagac
      601 tattttgttc cagacaatga attaccccca ttagtacaca gtggtttcaa tccttcattt
      661 attgcaactg tttctcatga aaaaggctca ggagatacaa gtgaatttga aataacgtat
      721 ggcagaaata tggatgttac tcatgctact agaagaacaa cacactatgg caatagttat
      781 ttagaaggat ctagaataca caacgcattt gtaaacagaa attacacagt taaatatgaa
      841 gtgaactgga aaactcatga aattaaagtg aaaggacata attgatatga aaaaaatagt
      901 caaatcatca gttgttacat caattgcatt gcttttgcta tccaatacag ttgatgcagc
      961 tcaacatatc acacctgtaa gtgagaaaaa ggttgatgat aaaattactt tgtacaaaac
     1021 aactgcaaca tcagattccg ataagttaaa aatttctcag attttaactt ttaattttat
     1081 taaagataaa agttatgata aagatacatt aatactcaaa gctgctggaa acatttattc
     1141 tggctataca aagccaaatc caaaagacac tattagttct caattttatt ggggttctaa
     1201 gtacaacatt tcaattaatt cagattctaa tgactcagta aacgttgtag attatgcacc
     1261 taaaaatcaa aatgaagaat ttcaagtaca acaaacggta ggttattctt atggtggaga
     1321 tattaatatc tctaacggct tatcaggtgg aggtaatggt tcaaaatctt tttcagagac
     1381 aattaactat aaacaagaaa gctatagaac tagcttagat aaaagaacta atttcaaaaa
     1441 aattggttgg gatgttgaag cacataaaat tatgaataat ggttggggac catatggcag
     1501 agatagttat cattcaactt atggtaatga aatgttttta ggctcaagac aaagcaactt
     1561 aaatgctgga caaaacttct tggaatatca caaaatgcca gtgttatcca gaggtaactt
     1621 caatccagaa tttattggtg tcctatctcg aaaacaaaac gctgcaaaaa aatcgaaaat
     1681 tactgttact tatcaaagag aaatggatag atatacaaac ttttggaatc aacttcactg
     1741 gataggtaat aattataaag atgaaaatag agcaactcat acatcaattt atgaagttga
     1801 ttgggaaaat catacagtta aattaataga tactcaatct aaggaaaaaa atcctatgag
     1861 ctaaacagat agataatcaa aaaatcttaa atatgttaaa atttacaaac actttctttc
     1921 tatattaggg taaccacgtc ttaattgacg tggttatttt ttcagggcaa aaaaagggcg
     1981 gattatttaa ataaggacaa acacttgtag aaaatttaaa aggttaaaaa taataaagaa
     2041 cttggtataa caagggtttt atacatttgc gtacaacgac gaaatgtcaa tttaccatca
     2101 cattatgacg atatgtttat tttaaacaca caagctcatg cgcgtcttga tcaaatggca
     2161 caacagtttg aagttgtttg taatggcttg aacgaaaatg aaggacaagc aattcaaacg
     2221 atggatcaat ctgcctctct aatacggtca aacttaattc aagttaaaga acaattagaa
     2281 aaactagctg tatactaatt aatttattaa atgctacttg ttttcattga gaataagtag
     2341 ttttttcaaa cataaaagtt ttacaaacac ataaatgggt gatgagctat gtttaaaaga
     2401 actaaactaa tcttaatagc aacgattcta ctatcaggat gttcaactac caataacgaa
     2461 tccaacaatg aaacaaaatc tgtaccagaa gaaatggaag cttcaaaata tgtaggtcaa
     2521 ggcttccaac cacctgcaga aaaagatgcg gttgaatttg cgaaaaagca taaagataaa
     2581 attgctaaac gtggcgaaca attttttatg gataactttg gactaaaagt taaagctaca
     2641 aatgttgtag gtagtggcga cggtgtagaa gtattcgtgc attgtgatga ccacgacatc
     2701 gtatttaatg caagtattcc atttgacaaa tcaattattg ac
//
LOCUS       DAMZKC010000227         2740 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.230, whole genome shotgun sequence.
ACCESSION   DAMZKC010000227 DAMZKC010000000
VERSION     DAMZKC010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.230"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            348..857
                     /locus_tag="POB55_002503"
     CDS             348..857
                     /locus_tag="POB55_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6501440.1"
                     /translation="MIRLGKMSDLDQILNLVEEAKELMKEHDNEQWDDQYPLLEHFEE
                     DIAKDYLYVLEENDKIYGFIVVDQDQAEWYDDIDWPVNREGAFVIHRLTGSKEYKGAA
                     TELFNYVIDVVKARGAEVILTDTFALNKPAQGLFAKFGFHKVGEQLMEYPPYDKGEPF
                     YAYYKNLKE"
     gene            869..1939
                     /locus_tag="POB55_002504"
     CDS             869..1939
                     /locus_tag="POB55_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDI6501441.1"
                     /translation="MTKIAFTGGGTVGHVSVNLSLIPTALSQGYEALYIGSKNGIERE
                     MIESQLPEIKYYPISSGKLRRYISLENAKDVFKVLKGILDARKVLKKEKPDLLFSKGG
                     FVSVPVVIAAKSLNIPTIIHESDLTPGLANKIALKFAKKIYTTFEETLNYLPKEKADF
                     IGATIREDLKNGNAHNGYQLTGFNENKKVLLVMGGSLGSKKLNSIIRENLDALLQQYQ
                     VIHLTGKGLKDAQVKKSGYIQYEFVKEDLTDLLAITDTVISRAGSNAIYEFLTLRIPM
                     LLVPLGLDQSRGDQIDNANHFADKGYAKAIDEEQLTAQILLQELNEMEQERTRIINNM
                     KSYEQSYTKEALFDKMIKDALN"
     gene            1956..2570
                     /locus_tag="POB55_002505"
     CDS             1956..2570
                     /locus_tag="POB55_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDI6501442.1"
                     /translation="MSQWKRISLLIVFTLVFGIIAFFHESRLGKWIDNEVYEFIYSSE
                     SFITTSIMLGATKVGEVWAMLCISLLLVAYLMLKRHKIEALFFALTMALSGILNPALK
                     NIFDRERPTLLRLIDITGFSFPSGHAMGSTAYFGSGIYLLNRLNQGNSKGILIGLCAA
                     MILLISISRVYLGVHYPTDIIAGIIGGVFCIILSTLLLRNKLIN"
BASE COUNT          997 a          351 c          498 g          894 t
ORIGIN      
        1 tttttaggga tgtatgtccc agcctcggcg tgtttatttt tattgacgtc taactctaaa
       61 agatataaat tagacattta taaatgatgt aaataacgca atttctatca ttgctgttaa
      121 taattcatgg tttaatatac tatgagcata aactttttaa atagtattat tcactagttt
      181 taacaatcaa ttaattggta tatgatactt ttattggtta tttttatccc atagtgtgat
      241 aattactatt tttcattcat aataaaggtt taaagcatgt taatagtgtg taagattaac
      301 atgtactgaa aaacatgttt aacataacga tataaggagt gacgtacatg atccgtctag
      361 gtaaaatgtc agatttagat caaatcttaa atctagtaga agaagcaaaa gaattaatga
      421 aagaacacga caacgagcaa tgggacgatc agtaccctct tttagaacat tttgaagaag
      481 atattgctaa agattatttg tacgtattag aggaaaatga caaaatttat ggctttattg
      541 ttgtcgacca agaccaagca gaatggtatg atgacattga ctggccagta aatagagaag
      601 gcgcctttgt tattcatcga ttaactggtt cgaaagaata taaaggagct gctacagaat
      661 tattcaatta tgttattgat gtagttaaag cacgtggtgc agaagttatt ttaacggaca
      721 cctttgcgtt aaacaaacct gcacaaggtt tatttgcgaa atttggattt cataaggtcg
      781 gtgaacaatt aatggaatat ccgccatatg ataaaggtga accattttat gcatattata
      841 aaaatttaaa agaatagagg taatattaat gacgaaaatc gcatttaccg gagggggaac
      901 agttggacac gtatcagtaa atttaagttt aattccaact gcattatcac aaggttatga
      961 agcgctttat attggttcta aaaatggtat tgaaagagaa atgattgaat cacaactacc
     1021 agaaattaag tattatccta tttcgagtgg taaattaaga agatatattt ctttagaaaa
     1081 tgccaaagac gtatttaaag tattgaaagg tattcttgat gctcgtaaag ttttgaaaaa
     1141 agaaaaacct gatctattat tttcaaaagg tggatttgta tctgtgcctg ttgttattgc
     1201 agccaaatca ttaaatatac caactattat tcatgaatct gacttaacac caggattagc
     1261 gaataagata gcacttaaat ttgccaagaa aatatataca acatttgaag aaacgctaaa
     1321 ctacttacct aaagagaaag ctgattttat tggagcaaca attcgagaag atttaaaaaa
     1381 tggtaatgca cataatggtt atcaattaac aggctttaat gaaaataaaa aagttttact
     1441 cgttatgggt ggaagcttag gaagtaaaaa attaaatagc attattcgcg aaaacttaga
     1501 tgcattatta caacaatatc aagtgataca tttaactggt aaaggattaa aagatgctca
     1561 agttaaaaaa tcaggatata tacaatatga atttgttaaa gaggatttaa cagatttatt
     1621 agcaattacg gacacagtaa taagtagagc aggatcaaat gcgatttatg agttcttaac
     1681 attacgtata ccaatgttat tagtaccatt aggtttagat caatcccgag gcgaccaaat
     1741 tgacaatgca aatcattttg ctgataaagg ttatgctaaa gcgattgatg aagaacaatt
     1801 aacagcacaa attttattac aagaactaaa tgaaatggaa caggaaagaa ctcgaattat
     1861 caataatatg aaatcgtatg aacaaagtta tacgaaagaa gctttatttg ataagatgat
     1921 taaagatgca ttgaattaat ggggggtaat gctttatgag tcaatggaaa cgtatctctt
     1981 tgctcatcgt ttttacattg gtttttggaa ttatcgcgtt tttccacgaa tcaagactag
     2041 ggaaatggat tgataatgaa gtttatgaat ttatatattc atcagagagc ttcataacga
     2101 catctatcat gcttggggct actaaagtag gtgaagtctg ggcaatgtta tgtatttcat
     2161 tacttcttgt ggcatatctc atgttaaagc gccacaaaat tgaagcatta ttttttgcat
     2221 taacaatggc attatctgga attttgaatc cagcattaaa aaatatattc gatagagaaa
     2281 gacctacatt gctgcgttta attgatataa caggatttag ttttcctagc ggtcatgcta
     2341 tgggatcaac tgcatatttt ggaagtggta tctatctatt aaatcgatta aatcaaggta
     2401 attcaaaagg tattcttata gggttatgtg cagctatgat tttattgatt tccatatcac
     2461 gtgtatatct aggtgtacat tatccaacag atattattgc cggcattatt ggtggcgtat
     2521 tttgcattat tttatcaacg ttattactta gaaataaatt aataaattaa atagtaaaaa
     2581 aacaaaagca gtaaacctaa agtgtcgtaa gggtttactg cttttataaa acgttgttat
     2641 aacgtatatt gtcttttacg ggcatataaa aggggaatat ttgaaaatga ccaatccaac
     2701 aagaacgaaa cgttgtgggg ggatgttcta tgtggtattg
//
LOCUS       DAMZKC010000228         2725 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.231, whole genome shotgun sequence.
ACCESSION   DAMZKC010000228 DAMZKC010000000
VERSION     DAMZKC010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.231"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            155..358
                     /locus_tag="POB55_002506"
     CDS             155..358
                     /locus_tag="POB55_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterile alpha motif-like domain-containing
                     protein"
                     /protein_id="HDI6501443.1"
                     /translation="MTFYNFIMGFQNDNTPFGILAEHVSEDKAFPRLEERHQVIRAYV
                     MSNYTDHQLIETTNRAISLYMAN"
     gene            complement(355..591)
                     /locus_tag="POB55_002507"
     CDS             complement(355..591)
                     /locus_tag="POB55_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501444.1"
                     /translation="MDNHNQCNYVNPQNVSLDWECFIISKSEMLLDGVPNELINTWLD
                     KDIITPFSIRNDEINFKTKDIWDALIHHNWYYSN"
     gene            815..1396
                     /locus_tag="POB55_002508"
     CDS             815..1396
                     /locus_tag="POB55_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDI6501445.1"
                     /translation="MAKTLYLMRHGQTLFNFKGLIQGFGDSPLTELGIAQAQKARSYY
                     ETKGINFDLYASSTQERASDTLENVAPNQSYQRFKGLKEWHFGLFEGESVYLFDNLYK
                     PEDLFGDRIVPFKGEARQQVEDRIVKTLHDIMSQTKNNALVVSHGTIMGVFLRYCLKL
                     DEALKHNIGNCNILKFEYDNGTFKFVELIDPNL"
     gene            complement(1445..2062)
                     /locus_tag="POB55_002509"
     CDS             complement(1445..2062)
                     /locus_tag="POB55_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid transporter"
                     /protein_id="HDI6501446.1"
                     /translation="MVTAIIHGFILAIGLILPLGAQNVFIFNQGANQPKYRYVLPAII
                     AAGLSDSLLIIIAVVGVSIIIMSLPVLQAIIYIVGLIFLMYMAWTIWHDKPSTDGEAQ
                     IMSPMKQVSFALSVSLLNPHAILDTIGVIGSSAALYSGSNKIAFTIACISVSWLWFFL
                     LAILGEMVGSIDKTGKLLTIINKLSSIIIIIVALMILQKLIQLLF"
     gene            complement(2217..2711)
                     /locus_tag="POB55_002510"
     CDS             complement(2217..2711)
                     /locus_tag="POB55_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6501447.1"
                     /translation="MIEIKTLTNNDFNEYKRLVSTVNEEFTQDSHYSQTMTDTLIHDI
                     LNQGSPKCIVFGCYENETLIATAALEQIRYVGKEHKSLIKYNFVTNNDKSINSELINF
                     IINYARQNNYESLLTSIVSNNIGAKVFYSALGFDILGFEKNAIKIGNTYFDEHWLFYD
                     LINK"
BASE COUNT          981 a          412 c          412 g          920 t
ORIGIN      
        1 aaaactctta aagttgtata attcgttaat atttttagaa tcagataaca aaaaatgtgc
       61 aaaatcactt ttgcacattt acaaatacta gttttctgat aagattaata ataacacttt
      121 aaatagcgct taataaatga agggggcaag tcttatgacg ttttacaatt tcatcatggg
      181 ttttcaaaat gataacacac catttggtat attggccgaa cacgttagtg aagataaagc
      241 attccctcga ttagaagaaa gacaccaagt aattagagca tatgtgatgt ctaattacac
      301 agatcatcaa ttaattgaaa ctacaaatag agctattagc ttatatatgg caaattaatt
      361 tgagtagtac caattatgat gtattagtgc atcccaaata tcttttgttt taaagtttat
      421 ttcatcattt cttatcgaaa atggtgtaat aatgtcttta tctaaccaag tgttgataag
      481 ttcatttggt acaccatcta acaacatttc acttttacta attataaaac attcccagtc
      541 aagtgaaaca ttttgtggat tcacataatt acattgatta tgattatcca taaacactca
      601 ctcctttaaa attctgtact cttcattgcg ttttaccccg tcacattatc ttttaaacta
      661 aaatcatcat tacttatgaa aaaatgtaca tcaaaagcaa aggttttcgc taccgaaaaa
      721 gtttaaataa tggtttaata tatttggtac tcattttaat aaaaagagaa tacattttga
      781 gctatcaata ctttttattg aagaggtgtt attgttggct aaaacgttat atttaatgcg
      841 ccacggacaa actttgttta attttaaggg actaattcag ggatttggag attcgccgct
      901 aacagaactt ggaattgctc aagctcaaaa ggcacgtagt tattatgaaa ctaaggggat
      961 aaacttcgat ttatatgcat catcaacgca agaacgcgca agtgacacac ttgaaaatgt
     1021 tgcacctaac caatcgtatc aacgttttaa gggactgaag gaatggcatt ttggattatt
     1081 tgaaggtgag tcagtctatc tatttgataa tctatacaag cctgaagact tattcggaga
     1141 tcgaattgtt cctttcaaag gagaggcaag gcaacaagtt gaagatcgca ttgtgaaaac
     1201 tttacatgac attatgtctc aaacaaagaa taatgcatta gtcgtgagtc atggaacaat
     1261 aatgggagta tttttaagat attgccttaa actagatgaa gcattaaagc ataatatcgg
     1321 taattgtaat atcctgaaat ttgaatatga caatggaaca tttaaatttg ttgagttaat
     1381 tgatccaaat ttataataaa taaagcgtta tacatagaca caagcatgtc ttttgtataa
     1441 cgctttagaa taataattga ataagttttt gtaatatcat cagagcaaca ataataatta
     1501 ttatgcttga taatttattg attattgtta ataattttcc ggttttatca attgatccaa
     1561 ccatttctcc taaaattgcg agtaaaaaga accataacca tgaaacgcta atacaagcga
     1621 ttgtaaacgc aattttattg ctgccactat ataatgcagc actactacca attacaccaa
     1681 ttgtatctaa aatagcatgt ggattgagta atgaaactga taaagcaaaa cttacttgtt
     1741 tcattggaga cataatttga gcttctccat ctgttgaggg tttatcatgc caaatggtcc
     1801 aagccatata catcaagaaa attaaaccaa ctatataaat aattgcttga agtacaggta
     1861 aagacataat aatgatagat actcctacca ctgcaataat aataagtaag ctgtctgaca
     1921 acccggctgc aattattgca ggcaatacat atctatattt tggttgatta gctccttggt
     1981 taaaaataaa tacattttgt gcacctaaag gtaaaattag accaatcgct aagataaatc
     2041 catgaataat tgcggttacc attaagtttt gacacctcta aaacatagaa tattctgaaa
     2101 aaacttattg ttatcatatc aaataaaaaa caactactca atagtagagt agttgtatga
     2161 aaaatagaac ctatatgatt tgaatttatg agaaatgtag aatatataaa actgtattac
     2221 ttattaatca aatcataaaa aagccaatgt tcatcgaaat aggtatttcc gattttaatt
     2281 gcatttttct caaaaccaag aatgtcgaat cctagtgcac tatagaaaac tttagcacct
     2341 atgttgtttg acacaattga tgtaagtaaa gattcgtaat tgttctgccg tgcataatta
     2401 ataatgaaat taatgagctc gctattaatc gatttatcgt tattagtaac aaagttgtat
     2461 ttaattaatg atttatgttc ttttccaacg tatcgaattt gttctaaggc agctgttgcg
     2521 ataagtgttt cgttttcata acagccaaat acaatacatt tcggtgaacc ttgatttaaa
     2581 atgtcatgta ttaaggtgtc agtcattgtt tgactataat gtgaatcttg agtgaattct
     2641 tcattgactg tcgaaacaag tctcttatac tcattaaaat cattattcgt taatgtttta
     2701 atttctatca ttactgacct cctta
//
LOCUS       DAMZKC010000229         2725 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.232, whole genome shotgun sequence.
ACCESSION   DAMZKC010000229 DAMZKC010000000
VERSION     DAMZKC010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.232"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(275..697)
                     /locus_tag="POB55_002511"
     CDS             complement(275..697)
                     /locus_tag="POB55_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HDI6501448.1"
                     /translation="MAIHYETKATNVGGRKGHVYTDDRALDIDIVSPAQADGKATNPE
                     QLFAAGYASCFNGAFDLILKQNKVRDAHPEVTLTVRLEDDSDSESPKLSVSIDATIKN
                     VISQEEAEKYLQMAHEFCPYSKATQGNINVDLNVNVVD"
     gene            845..1561
                     /locus_tag="POB55_002512"
     CDS             845..1561
                     /locus_tag="POB55_002512"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HDI6501449.1"
                     /translation="MTHVEVVATIAPQLSIEETLIQKINHRIDAIDVLELRIDQIENV
                     TVDQVAEMITKLKVMQDSFKLLVTYRTKLQGGYGQFINDLYLNLISDLANINGIDMID
                     IEWQADIDIEKHQRIITHLQQYNKEVVISHHNFESTPPLDELQFIFFKMQKFNPEYVK
                     LAVMPHNKNDVLNLLQAMSTFSDTMDCKVVGISMSKLGLISRTAQGVFGGALTYGCIG
                     EPQAPGQIDVTDLKAQVTLY"
     gene            1644..2183
                     /locus_tag="POB55_002513"
     CDS             1644..2183
                     /locus_tag="POB55_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HDI6501450.1"
                     /translation="MELQQAIANRRSVKKFKRDMHIDDALLYQAIEKAADAPNHGMRE
                     PWRVVHVPKDRLGDMSKDISKFAFPNELDKQQCHYDAVTKLGGMLLLILKTDPRQRQN
                     DENYFAFGAYAQNLMLLLYEAGIGTCWKSPLYIYDPKVRKTLGIKKDEVLSGFLYLTD
                     LEEDMPKAPRKNRNLITLY"
     gene            complement(2333..2653)
                     /locus_tag="POB55_002514"
     CDS             complement(2333..2653)
                     /locus_tag="POB55_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDI6501451.1"
                     /translation="MQAIKSNESFKSVINSDTPVIVKFEAGWCPDCRAMDLWIDPIVE
                     QYKDYQWYTVNRDELEDVVVENEVMGIPSLLVFKNGDKIAHLHSANAKSPEQVESFLA
                     ETFK"
BASE COUNT          943 a          406 c          464 g          912 t
ORIGIN      
        1 tcaatttaat tatatttatt tttaagttca ggaaattatg gaattggtta ttggtcgaca
       61 ctggactttt atttgtagac ggtatgcgaa gaagtcaagc cagtattgga accggtcaca
      121 tttatgatga ctaagggaca atcattttca gttaaatatt gccagaaatt tcagtcacga
      181 atagaaaaaa taaccaacca aaaatttttg gttggcaaat ttgaatttta tgtgaatttt
      241 attaataacg ttgaataatc tctttaagtt aatgctaatc tacaacattt acatttaaat
      301 cgacattaat atttccttga gtcgcttttg aatatggaca aaattcatga gccatttgta
      361 aatatttttc agcttcttct tgagatataa catttttaat tgtcgcatca attgaaacac
      421 ttaatttagg actttctgag tctgaatcat cttctagtct cactgttagt gttacttctg
      481 gatgagcatc acgcactttg ttttgcttta aaattaggtc gaaagcaccg ttgaagcaag
      541 atgcataacc tgctgcaaat aattgttcgg ggttagtagc tttaccatct gcttgagcag
      601 gcgaaacgat atcaatatct aacgctcgat catcagtata aacatgtcct ttacgtccgc
      661 caacattcgt cgctttagtt tcataatgta ttgccattct tataacctcc taatttaaaa
      721 tacatttatg ctttacccat tcgaaaatac cttaatcatt ttcatttata gcattgtttg
      781 attgaaggat aaaaagttgt tgtttacaat aaaaataatg agtatctgaa atgagggatt
      841 tactatgaca catgtggaag tagtagcgac tatcgcgcca caattatcta tcgaagaaac
      901 tttaattcaa aaaattaatc atcgtattga tgcaatagac gtattagaat tacgaattga
      961 tcaaattgaa aatgtcacag ttgatcaagt ggcagaaatg attacaaagc tgaaggttat
     1021 gcaagattca ttcaaattat tagttacgta tcgtacaaag ttacaaggtg gctatgggca
     1081 atttataaat gacttgtatc ttaatttaat atcagactta gcaaatatca atggcataga
     1141 tatgattgat atagaatggc aagcagatat tgacattgaa aaacatcaac gaatcattac
     1201 acacttgcaa cagtataata aagaggtggt tatatcacat cataatttcg aaagtacgcc
     1261 tccattagat gaattgcaat ttatattttt taaaatgcaa aaattcaacc cagaatacgt
     1321 taaattagca gtaatgccac ataataaaaa tgatgtgtta aatttattgc aggcaatgtc
     1381 tacattttca gatactatgg actgcaaagt tgttggtatt tcaatgtcta aacttggact
     1441 aataagtaga acggctcaag gcgtttttgg tggtgcattg acttatggtt gtatcggaga
     1501 accacaagct ccaggacaga ttgatgttac tgatttaaaa gcacaagtga ctttatacta
     1561 ataaaatgaa ttaggttgct tttgtgtaag tgctgtctta taattgagaa gtattatcat
     1621 tgttcgtgga ggagcgattt tagatggaat tacaacaagc aatagctaat agaagaagtg
     1681 tgaaaaaatt taaaagagat atgcacatag atgacgcatt gctatatcaa gcaattgaga
     1741 aagctgctga tgctccaaat cacggaatga gggaaccatg gagagttgtg catgttccga
     1801 aagacagatt aggagatatg agtaaggata tttctaaatt tgcatttcct aatgaattag
     1861 ataagcaaca atgtcattat gatgcagtta cgaaactagg tggcatgtta ttgcttattt
     1921 taaaaacaga tccaagacaa cgtcaaaatg atgaaaacta ctttgcattt ggtgcatatg
     1981 cacaaaatct tatgttgtta ctttatgaag cgggaatagg tacatgttgg aaatcgccat
     2041 tatatatcta tgatcctaaa gtaagaaaaa cacttggtat aaagaaagat gaagttcttt
     2101 ctggattctt atatttaacg gatttagaag aagatatgcc taaagcacca cgtaaaaata
     2161 gaaacttaat tacattatat taatatgtat aattatagaa acattaataa aagctgagtc
     2221 atgaattgat ggacatctat cgagttagag atttaatcta acttactaga gtcggtacaa
     2281 tcacagtctc agctttttat tgtgcagtat atacacattt ttattttagt atttatttaa
     2341 aagtttctgc taaaaatgat tcaacttgct caggtgactt agcatttgct gaatgaaggt
     2401 gtgcaatttt gtcgccgttt ttaaatacta gcaagctagg gatacccata acttcatttt
     2461 caacaactac atcttctaat tcatcacgat taacagtata ccattggtaa tctttatatt
     2521 gttctacgat tgggtcaatc cataaatcca tagcacgaca gtctgggcac catcctgcct
     2581 caaatttaac aattacaggt gtatcgctat taatcacaga tttaaatgat tcattacttt
     2641 tgattgcttg cattgtaaca actcctctag atagtttaat aatttttatt atagctaaat
     2701 ttataacata ataaaaaatt ttagc
//
LOCUS       DAMZKC010000230         2716 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.233, whole genome shotgun sequence.
ACCESSION   DAMZKC010000230 DAMZKC010000000
VERSION     DAMZKC010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.233"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            7..513
                     /locus_tag="POB55_002515"
     CDS             7..513
                     /locus_tag="POB55_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501452.1"
                     /translation="MIIKILTILLLLCILSYLVTNRKKPFLFLKTLFMGVVFIFIGYI
                     SLAISAVIIYGIIQFITIDFGSFFLMGIILILISSIFQLFIVRLLFRKKNVDLTEVVV
                     LEHLIQWFLVYFAIYQAVNEKMDINDINIDNFQSVFFDVSNLNLVILPTLIISWVTIF
                     NYRMRSYK"
     gene            772..1269
                     /gene="efb"
                     /locus_tag="POB55_002516"
     CDS             772..1269
                     /gene="efb"
                     /locus_tag="POB55_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement convertase inhibitor Efb"
                     /protein_id="HDI6501453.1"
                     /translation="MKNKLIAKSLLTIAAIGITTTTIASTADASEGYGPREKKPVSIN
                     HNIVEYNDGTFKYQSRPKFNSTPKYIKFKHDYNILEFNDGTFEYGARPQFNKPAAKTD
                     ATIKKEQKLIQAQNLVREFEKTHTVSAHRKAQKAVNLVSFEYKVKKMVLQERIDNVLK
                     QGLVK"
     gene            1423..1773
                     /gene="scb"
                     /locus_tag="POB55_002517"
     CDS             1423..1773
                     /gene="scb"
                     /locus_tag="POB55_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement inhibitor SCIN-B"
                     /protein_id="HDI6501454.1"
                     /translation="MKFKKYILTGTLALLLSSTGIATIEGNKADASSLDKYLTESQFH
                     DKRIAEELRTLLNKSNVYALAAGSLNPYYKRTIMMNEYRAKAALKKNDFVSMADAKVA
                     LEKIYKEIDEIINR"
     gene            complement(2538..2711)
                     /locus_tag="POB55_002518"
     CDS             complement(2538..2711)
                     /locus_tag="POB55_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501455.1"
                     /translation="MNKKATKLLEDYKKVQQRKSEETDRGCLKSVIMLIVLFFLIIFG
                     IVACSTNVNFFFQ"
BASE COUNT         1036 a          367 c          432 g          881 t
ORIGIN      
        1 caaacgatga taattaaaat tttaacaatt ctattactac tttgtatatt gagctatttg
       61 gttacaaata gaaagaagcc ttttctgttc ttaaaaacac tctttatggg tgtggtattt
      121 atctttatag gatatatttc actggcaata tctgccgtaa ttatttatgg cattattcaa
      181 tttatcacaa ttgattttgg tagttttttc ttaatgggta ttatattaat cttgatttca
      241 agtatattcc aattatttat agttagatta ctttttagaa aaaagaatgt cgatttgaca
      301 gaggttgtcg ttttagaaca tttaattcaa tggttcttag tttactttgc gatctatcaa
      361 gcagtaaatg aaaaaatgga cattaatgat attaatatcg acaattttca atctgtcttt
      421 tttgacgtgt ctaatttgaa tttagtaatt ctaccaacgt taatcattag ctgggtcaca
      481 atatttaact atagaatgag aagttacaaa taaaatcaat gaaattatac catcagacac
      541 caacattcaa atggtgtctt ttttgttgtg tggttttatt tttgaaattc gaaaaagtag
      601 aggcatgaat tttttgacta gtgtataagt gctgatgaga cacaagatag ataactatat
      661 tttgtctata ttataaagtg tttatagtta attaataatt agttaatttc aaaagttgta
      721 taaataggat aacttaataa atgtaagata ataatttgga ggataattaa catgaaaaat
      781 aaattgatag caaaatcttt attaacaata gcggcaatag gtattactac aactacaatt
      841 gcgtcaacag cagatgcgag cgaaggatac ggtccaagag aaaagaaacc agtgagtatt
      901 aatcacaata tcgtagagta caatgatggt acttttaaat atcaatctag accaaaattt
      961 aactcaacac ctaaatatat taaattcaaa catgactata atattttaga atttaacgat
     1021 ggtacattcg aatatggtgc acgtccacaa tttaataaac cagcagcaaa aactgatgca
     1081 actattaaaa aagaacaaaa attgattcaa gctcaaaatc ttgtgagaga atttgaaaaa
     1141 acacatactg tcagtgcaca cagaaaagca caaaaggcag tcaacttagt ttcgtttgaa
     1201 tacaaagtta agaaaatggt cttacaagag cgaattgata atgtattaaa acaaggatta
     1261 gttaaataaa acttcaatcg ttgctgttat ctggaaataa ttaattaaat gttatgttaa
     1321 tttttgttaa tgaaaaaagt aatctattta atgacaggtt aatgtaattg tcctgaaatt
     1381 gactatatac tcagtaagta tcaattttaa ggagagctta taatgaaatt taaaaaatat
     1441 atattaacag gaacattagc attactttta tcatcaactg ggatagcaac tatagaaggg
     1501 aataaagcag atgcaagtag tctggacaaa tatttaactg aaagtcagtt tcatgataaa
     1561 cgcatagcag aagaattaag aactttactt aacaaatcga atgtatatgc attagctgca
     1621 ggaagcttaa atccatatta taaacgtacg attatgatga atgaatatag agctaaagcg
     1681 gcacttaaga aaaatgattt cgtatcaatg gctgatgcta aagttgcatt agaaaaaata
     1741 tacaaagaaa ttgatgaaat tataaataga taataaataa aacaggttga gacaaaaaat
     1801 ggtcttaacc tgttttcaat ttgcatatgt gataaattct atatcaaaat gcttatgtat
     1861 aatgaatgac atttaaaagt aggggagaca aatataaata caatagttcc taggattact
     1921 ctcaaaataa ctatatcaat tatttacttt gctctcctat tttttaaaat atgtacatgt
     1981 ttaaacaatc aaaagtgtac aatattaaat tatcatttcc agttctagtg ctatattggt
     2041 agtagttgac taaatgaaaa taagcttata acaagttttt tcaatactcg tggggccaca
     2101 acagagagaa ataggatcac caattccaac agacaatgca agttggcggg gccccaacat
     2161 agagaaattg gatcaccaat ttcaacagac aatgcaagtt ggggtggggc ccaacacaga
     2221 agctggcgaa aagtcagcat acaaaaatgt gcaagttggc ggggccacaa cagagagaaa
     2281 taggatcacc aattccaaca aacaatgcaa gttggcgggg ccccaacata gagaaattgg
     2341 gtcaccaatt tcaacagaca atgcaagttg gggtaggaca tcgataaaaa aatacttttt
     2401 ctttagaaat tagtatttct tatgcatgag ttttactcat gtattcattt tttaagtaga
     2461 cattagctac agctaatgat aaagaaccac tacataataa atcattagtg gttctttatc
     2521 atttctatct cactctttta ctggaagaaa aagtttacgt ttgtagaaca tgccacaata
     2581 ccaaaaataa ttaagaaaaa taagacgata agcatgatga cacttttcaa acaacctcta
     2641 tcagtttctt ccgattttct ttgttgaacc tttttataat cttcaagtag ttttgtcgct
     2701 tttttattta tatgtt
//
LOCUS       DAMZKC010000231         2693 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.234, whole genome shotgun sequence.
ACCESSION   DAMZKC010000231 DAMZKC010000000
VERSION     DAMZKC010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.234"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(<1..1394)
                     /locus_tag="POB55_002519"
     CDS             complement(<1..1394)
                     /locus_tag="POB55_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HDI6501456.1"
                     /translation="MEIFETILIFIAVVILSSFVHTFIPKVPLAFIQIFLGMLLFITP
                     IPVQFNFDSELFMVTMIAPLLFVEGVNVSRVHLRKYIKPVMMMALGLVITTVIGVGLF
                     IHWIWPDLPIGAAFAIAAILCPTDAVAVQAITKGKVLPKGAMTILEGESLLNDAAGII
                     SFKIAVGVLVTGAFSLVDAVQLFLIASIGGAVVGLLIGMALVRFRLTLMRRGYENINM
                     FTIIQLLTPFVTYLIAELFHASGIIAAVVAGLVHGFERDRIMQVRTQLQMSYNHTWNI
                     LGYVLNGFVFSILGFLVPEVIIKIIKTEPHNLIFLIGITIVVALAVYLFRFVWVYVLY
                     PYFYLAISPFQKMMTKNDDDNPTTEKPPKRSLYALIMTLCGVHGTISLAIALTLPYFL
                     AGHHAFTYRNDLLFIASGMVIISLVVAQVLLPLLTKPAPKTVIGNMSFKVARIYILEQ
                     VIDYLNQKSTFETS"
     gene            complement(1734..2171)
                     /gene="mnhG2"
                     /locus_tag="POB55_002520"
     CDS             complement(1734..2171)
                     /gene="mnhG2"
                     /locus_tag="POB55_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit G"
                     /protein_id="HDI6501457.1"
                     /translation="MEITKEIFSLIAAVMLLLGSFIALISAIGIVKFQDVFLRSHAAT
                     KSSTLSVLLTLIGVLIYFIVNTGFFSVRLLLSLVFINLTSPVGMHLVARAAYRNGAYM
                     YRKNDAHTHASILLSSNEQNSTEALQLRAEKREEHRKKWYQND"
     gene            complement(2146..2448)
                     /gene="mnhF2"
                     /locus_tag="POB55_002521"
     CDS             complement(2146..2448)
                     /gene="mnhF2"
                     /locus_tag="POB55_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit F"
                     /protein_id="HDI6501458.1"
                     /translation="MIQTITHIMIISSLIIFGIALIICLFRLIKGPTTADRVVTFDTT
                     SAVVMSIVGVLSVLMGTVSFLDSIMLIAIISFVSSVSISRFIGGGHVFNGNNKRNL"
     gene            complement(2445..>2693)
                     /gene="mnhE2"
                     /locus_tag="POB55_002522"
     CDS             complement(2445..>2693)
                     /gene="mnhE2"
                     /locus_tag="POB55_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit E"
                     /protein_id="HDI6501459.1"
                     /translation="YILFKTKDMNPGLLSYETRLTSDWSITFLTILIIITPGSTVIRI
                     SQDSKKFFIHSIDVSEKEKDSLLRSIKHYEDLILEVSR"
BASE COUNT          996 a          475 c          380 g          842 t
ORIGIN      
        1 aaacttgttt cgaaagtaga tttttgattt agataatcaa taacttgttc taatatataa
       61 attctagcaa ctttaaacga catattgcca attactgttt taggtgcagg tttcgttaat
      121 aatggcaata atacttgcgc aactaccaaa ctaataataa ccataccaga tgcaataaat
      181 aataagtcgt ttctatacgt aaaagcatga tgccctgcta aaaaatacgg taacgttaat
      241 gcgattgcta aagaaattgt tccatgcaca ccacataacg tcataattaa agcgtataaa
      301 cttcgctttg gtggtttctc agtcgttgga ttatcatcat catttttagt catcattttt
      361 tggaatggac tgatggctaa ataaaaataa ggatataaga cataaaccca aacaaatcta
      421 aatagataga cagctaaagc aacaacaata gtgatgccta ttaaaaagat taaattgtgc
      481 ggttctgttt tgataatttt aataataact tcaggtacta aaaatcctaa tattgaaaaa
      541 acaaagccat ttaaaacata acctagtata ttccatgtat gattgtaact catttgcagt
      601 tgtgtacgta cttgcataat tctgtcacgt tcgaaaccat gtacaagtcc tgcaactact
      661 gctgcaatga ttcctgatgc gtgaaacaat tcagcaatta aatacgtaac aaatggtgtt
      721 aacaattgaa taattgtaaa catattaatg ttttcatatc ctcgacgcat caatgttaat
      781 cggaacctta ctaatgccat acctataagt aaaccaacca ctgcgccacc aattgatgca
      841 attaaaaaca actgaacagc atcaacaagt gaaaaagcac ctgtaactaa tactccaaca
      901 gctattttaa atgaaataat accagcagca tcattcaata atgactcacc ttcaagaatt
      961 gtcattgctc cttttggcaa gacctttcct ttagtgattg cttgcactgc tactgcatca
     1021 gtaggacaaa gaatggcagc aattgcaaat gctgctccaa taggtaaatc tggccaaatc
     1081 caatgaataa ataaacctac acctatcaca gtagtaatga ctaatcctaa tgccatcatc
     1141 atcactggct taatatattt ccttaaatgg actctagaaa cattaacacc ttctacaaat
     1201 aacaaaggcg caatcattgt taccataaac aattcagaat caaaattaaa ttgaacaggg
     1261 attggggtaa taaatagtaa catgcccaag aaaatttgta taaatgctag gggtacttta
     1321 ggtatgaaag tatggacaaa cgaacttagt atcacaacag ctataaatat aagaattgtt
     1381 tcaaatattt ccaaactttc acctctctaa aaagtattgt ttaattgaaa attaagtatc
     1441 acatctcgtt gtaattatac tttagaggat aaattgagtt agcgaccaca aaagcacttt
     1501 aatatagata tatgtctacg attgcagtac ttaaatttgc aattatttaa ttttatttta
     1561 tcactaattg tttgtataaa taaacaactt gctttcacat aacaacatta acttataata
     1621 caaaaaatga gcaccttaaa atcgactaac caatttcaaa gtactctttt aatgattaat
     1681 tttgaaaaca gattttcaaa agcattgtta tgcttaacaa tttagccaac acttcaatcg
     1741 ttttgatacc atttcttacg atgctcttct cgtttttcag cacgtaattg taatgcttct
     1801 gtagagtttt gttcatttga acttaataat attgatgcat gtgtgtgagc atcatttttt
     1861 cgatacatat aagcgccgtt gcgataagca gcgcgagcga ctaagtgcat gccgactggt
     1921 gaagttaaat taataaaaac aagtgacagt aataaacgca cactgaaaaa tcctgtattc
     1981 acaataaaat aaattaatac accgattaaa gttaataaca cggataaagt tgaacttttt
     2041 gtcgcagcgt gacttcttaa gaaaacatct tggaatttca cgatacctat tgcactaata
     2101 agagcaataa aactacctaa caacaacatc acagcagcaa taagactaaa gatttctttt
     2161 gttatttcca ttaaacacat gccccccacc aataaagcgt gatattgaaa cagaacttac
     2221 aaaagatata atggcaatga gcatgattga atctaagaaa gaaacggtgc ccataagtac
     2281 acttaacaca cccacaattg acattacgac agcacttgtt gtatcaaatg taacgacacg
     2341 atctgctgtt gtaggtccct tgattaatct aaataaacag atgattaatg caattccaaa
     2401 aataatgagt gaactaataa tcataatatg tgttattgtt tgtatcatcg cgacacctcc
     2461 aatattaagt cttcataatg cttaatactt cttaacaaac tatctttttc tttttctgac
     2521 acgtcgatac tatgaataaa aaacttttta gagtcttgag aaattcgtat tactgtagac
     2581 cctggagtta taataattaa aattgttaaa aatgttattg accaatcact tgttagtctt
     2641 gtttcatatg aaagtaatcc agggttcata tcttttgttt taaaaagaat ata
//
LOCUS       DAMZKC010000232         2651 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.235, whole genome shotgun sequence.
ACCESSION   DAMZKC010000232 DAMZKC010000000
VERSION     DAMZKC010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.235"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(98..1141)
                     /gene="recA"
                     /locus_tag="POB55_002523"
     CDS             complement(98..1141)
                     /gene="recA"
                     /locus_tag="POB55_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HDI6501460.1"
                     /translation="MDNDRQKALDTVIKNMEKSFGKGAVMKLGDNIGRRVSTTSTGSV
                     TLDNALGVGGYPKGRIIEIYGPESSGKTTVALHAIAEVQSNGGVAAFIDAEHALDPEY
                     AQALGVDIDNLYLSQPDHGEQGLEIAEAFVRSGAVDIVVVDSVAALTPKAEIEGEMGD
                     THVGLQARLMSQALRKLSGAISKSNTTAIFINQIREKVGVMFGNPETTPGGRALKFYS
                     SVRLEVRRAEQLKQGQEIVGNRTKIKVVKNKVAPPFRVAEVDIMYGQGISKEGELIDL
                     GVENDIVDKSGAWYSYNGERMGQGKENVKMYLKENPQIKEEIDRKLREKLGISDGDVE
                     ETEDAPKSLFDEE"
     gene            complement(1306..2457)
                     /locus_tag="POB55_002524"
     CDS             complement(1306..2457)
                     /locus_tag="POB55_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HDI6501461.1"
                     /translation="MSIAIIAVGSELLLGQIANTNGQFLSKVFNEIGQNVLEHKVIGD
                     NKKRLESSVRHALEKYDTVILTGGLGPTKDDLTKHTVAQIVGKDLVIDEPSLKYIESY
                     FEEQGQEMTPNNKQQALVIEGSTVLTNHHGMAPGMMVNFENKQIILLPGPPKEMQPMV
                     KNELLSHFINHNRIIHSELLRFAGIGESKVETILIDLIDKQTNPTIAPLAGSHEVYIR
                     LTANADSKEQAQSLIQPVKQEILDRIGEYYYGSDDTLIEQAVIKKIHEPFVIYDGITN
                     GALYHRLKEVDLNDVLKGMINHNENFVDINKPIEQQLKDAVQFVNKLFNVSSAIILLE
                     YDGVVHIGYDNNFEFKTEQFKMSKSRNLLKNRSQNYALIRLLNWLRTTN"
BASE COUNT          818 a          507 c          352 g          974 t
ORIGIN      
        1 aagttagata ttattctagc tttatgagat gaatatcact ttcaatcaat cctataagga
       61 tatttgctaa gtttaactat agatataaat ttgtgtacta ttcttcgtca aataatgact
      121 ttggtgcatc ttctgtttct tcaacatcac catcagatat acctaatttt tctctcaatt
      181 tacgatcaat ttcttcttta atttgtggat tttctttcaa gtacatttta acattttcct
      241 taccttgacc cattcgttcg ccattgtaag aataccatgc tcctgattta tcaacgatgt
      301 cgttttcaac acctaaatca ataagttcac cctctttaga aataccttgt ccatacataa
      361 tgtcaacttc agctactcta aatggtggtg ccactttatt tttaacgact ttaattttag
      421 ttctattacc tacaatttct tgtccttgtt taagctgttc tgcacgacgt acttctagtc
      481 ttactgaact atagaatttt aatgcacgtc cacctggtgt agtctctgga ttaccgaaca
      541 taacaccaac tttttcacga atttggttga tgaaaatagc agttgtattt gatttagaaa
      601 tagcacctga aagtttacgt aacgcttgtg acattaaacg agcttgtaaa ccaacgtgag
      661 tgtctcccat ttctccttca atttcagctt taggtgttaa agcagcaact gagtctacaa
      721 ctacaatatc aactgcacca cttctaacaa atgcttcggc gatttcaaga ccttgttcac
      781 catgatccgg ttgcgataaa tataaattat cgatatctac gcctaatgct tgagcatatt
      841 ctggatctaa agcatgttca gcatcgataa atgctgccac cccgccatta ctttgtactt
      901 cagcaatagc gtgaagcgct actgttgtct taccagaact ttcaggacca taaatttcaa
      961 taattcgtcc tttaggatag ccacctacac ctagcgcatt atctaatgta actgaaccag
     1021 ttgatgtagt tgaaactcgg cgacctatat tgtcacccaa cttcattacg gcacctttac
     1081 cgaaagattt ctccatattt ttaattactg tatctaaagc tttttgacga tcgttatcca
     1141 aagcgagacc tcctaattga aattgctatc ttttaattat cttaccaata ctatacaagg
     1201 attcaaaata aaatacaagt gttttgcgaa tatttgttcg tataaaatct tgaaatcaaa
     1261 acgcaaaaac gaacaaacgt gcttatattt ttatcgataa tacaattaat ttgttgttct
     1321 aagccaattt aataatctta tgagcgcata attttgactt ctgttcttta ataaatttct
     1381 agatttagac attttaaatt gctcagtttt aaattcaaag ttattatcat agcctatatg
     1441 gactacacca tcatactcta atagaataat tgctgatgac acattaaaca atttattaac
     1501 aaattgcact gcatctttta attgctgctc aataggttta ttaatatcaa caaagttttc
     1561 attgtgatta atcataccct ttagaacatc gtttaaatcc acttctttca atcgatgata
     1621 taaagcacca ttagtaatac catcatatat tacaaaaggt tcatgaattt tctttattac
     1681 agcttgctca attaatgtgt catctgaacc ataataatat tctccaatac gatcaagaat
     1741 ttcttgttta acaggttgaa tcaatgattg tgcttgttct tttgagtcgg catttgcagt
     1801 caatctaata tatacttcat gacttcccgc caaaggcgca atcgtaggat tagtctgttt
     1861 atcgataaga tctattaata ttgtttctac tttagattca cctattcccg caaatcttaa
     1921 tagttcagaa tgtataattc gattatggtt tataaaatgt gacaacaatt catttttcac
     1981 cattggttgc atttctttcg gtggacctgg taataaaata atttgtttgt tttcaaaatt
     2041 caccatcatt cctggagcca tgccatgatg atttgttaat acagttgaac cttcaattac
     2101 taaagcctgt tgtttattat taggtgtcat ttcttgtcct tgttcctcaa aatagctttc
     2161 aatatatttt aaagaaggct catcaataac taaatcttta ccaacaatct gggccactgt
     2221 atgcttcgtt aagtcatctt tcgtaggacc taagccacct gttaaaataa cagtatcata
     2281 tttttctagc gcatgacgta cacttgattc taaacgtttt ttattatctc caataacttt
     2341 atgttctaat acattttgtc caatttcatt aaatacttta gatagaaatt gtccgttggt
     2401 attagcgatt tgacctagca atagttctga gcctacagca ataatggcaa ttgacattta
     2461 tatccccctt gtatcgttta attcattttt tcatgtattt catttaaaaa ttcacatcaa
     2521 ttttagattc aatgtatcag atgctactac agattttccc taaaaaaatg aacgaatatt
     2581 gttttacaat atatcattat aaacaattgc gtggctaatt tctaaaattg ggattaaaaa
     2641 aactagattc c
//
LOCUS       DAMZKC010000233         2578 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.236, whole genome shotgun sequence.
ACCESSION   DAMZKC010000233 DAMZKC010000000
VERSION     DAMZKC010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.236"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            70..435
                     /locus_tag="POB55_002525"
     CDS             70..435
                     /locus_tag="POB55_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HDI6501462.1"
                     /translation="MKKKKNAIKVIIILFISLIVAVAFFFGLKTYQGHKNIQLIDSYL
                     EEKNLKDKIKSEKTEYSAKKGLFYKEVTFKDEPGVTYVVQPISTNKGLFVEGFDTETK
                     KSLKTAKHKYFNQNYKPSK"
     gene            617..1894
                     /locus_tag="POB55_002526"
     CDS             617..1894
                     /locus_tag="POB55_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HDI6501463.1"
                     /translation="MALFKRKISLPMQVVIALVLGVVVGLLLYGQENVANYIKPFGDV
                     FLNLIKMIVIPVVFCSLALSISNVGESKTVGRYGWKTILYFEIITTIAIGLGIIFGNL
                     FKPGAGLDPTKLPKGDISKYQSTAHAAEQSTYGNHFIDTIVHIIPTNFFEALNKGELL
                     PIIFFAVFFGLGLAAVGKKAEPVKEFLSGSLEAVFWMINKILKLAPLGVFAFICTTII
                     TFGASALLPLLKLVLVVVFAMVFFVFAILGLVAWMCGINIMNIIRILKSELLLAFSTS
                     SSEAVLPVMMKKMENFGSPKEITSFVIPIGYTFNLDGSALYQSIAALFVAQMYGMHLT
                     LSEQIVLMLTLMITSKGMAAVPGTSIVVLLTTLGAMGLPAQGLALIIGVDRILDMVRT
                     CVNVIGNALSTIVIAKWENVYDKAKGQEYLKSI"
     gene            complement(2165..2521)
                     /locus_tag="POB55_002527"
     CDS             complement(2165..2521)
                     /locus_tag="POB55_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4889 domain-containing protein"
                     /protein_id="HDI6501464.1"
                     /translation="MKKKKGFGLGISLIAIMLIVCIVLVIMMMTGGKKDTYYGIMKDN
                     TTIEKMISEKDESIEKNVKLPSDSDVKVKKGDFVIVYKLADSDKIVKVKKVDHDDVPH
                     GLMMKIHDMGKMHMKH"
BASE COUNT          874 a          381 c          431 g          892 t
ORIGIN      
        1 tggtaaaaac tctttattaa ataaattgat tcgttcatac tgaatagata agcaaaaagg
       61 agttacataa tgaagaagaa aaaaaacgcg attaaagtta tcattatatt atttatttca
      121 ttaattgttg cagtagcatt tttctttggt ctgaaaacat atcaaggcca taagaatatt
      181 caactaattg acagttactt ggaagagaaa aatctaaaag ataaaattaa atcagaaaaa
      241 acagagtata gtgctaaaaa aggtctcttc tataaggaag ttacatttaa agatgaacct
      301 ggtgtaactt atgttgtgca accaatcagt actaataaag gattatttgt tgaaggcttt
      361 gatacagaga ctaagaaaag tttgaaaact gcaaagcata aatattttaa tcaaaattat
      421 aaaccatcaa aatagtgtaa cgtataactg gtgaaacagt ttagtgcctt caattttaat
      481 tatttgaggt aaacagtttt tgtatggaaa ttagtgattt ttgacatcta taaaagataa
      541 atattttaat tttttataga taattatcaa ttttctgata taattgagtg atgtcagatg
      601 aaggggagtt tgtactatgg ctctattcaa gagaaaaatt agcctaccta tgcaagttgt
      661 tattgcatta gtgttaggtg ttgtcgtagg acttttatta tatggacaag aaaacgtagc
      721 aaattacatt aaaccatttg gtgacgtatt tttaaattta attaaaatga tcgttatacc
      781 agttgtattt tgctcactag cgctttctat ttcgaacgtt ggggaatcga aaactgtagg
      841 gcgttatggc tggaaaacaa ttttatactt tgaaattatt acaacaatcg caataggttt
      901 agggattatc ttcggtaacc tatttaaacc aggtgctgga ttagacccaa caaaattacc
      961 taaaggtgat atttctaaat atcaatcaac tgcacatgca gcagaacaat ctacatatgg
     1021 aaatcatttt attgatacca ttgtacatat tattccgaca aacttttttg aagctttaaa
     1081 taagggtgaa ttattaccta ttatcttctt cgcagtattc tttggattag gattagctgc
     1141 tgtaggtaaa aaagcagaac cagttaaaga atttttaagc ggatcgcttg aagctgtgtt
     1201 ctggatgatt aataaaattt taaaattagc accacttgga gtgtttgcat tcatttgtac
     1261 tacaattatt acatttggtg catccgcatt attaccacta ttaaaattag tattagttgt
     1321 tgtctttgca atggtgttct ttgtattcgc tatactagga ctagttgcat ggatgtgtgg
     1381 tattaatatc atgaatatta ttagaatctt gaaaagtgaa ttgcttttag cattttctac
     1441 atcaagttcg gaagctgtac ttcctgtaat gatgaagaaa atggaaaact tcggttctcc
     1501 aaaagaaatt acttcttttg ttataccaat tggttatacg tttaacttag acggatcagc
     1561 actttatcaa tctattgcag cattattcgt tgcacagatg tatggaatgc acttaacatt
     1621 atcagagcaa attgtgttga tgttaacatt aatgattacg tctaaaggta tggcggcagt
     1681 accaggaact tcaatcgttg ttttattaac aacattaggt gccatgggct taccggcaca
     1741 aggtttagca ttaattattg gtgttgaccg tatcttagat atggtacgta catgtgtaaa
     1801 cgttattggt aacgcattat caacaatcgt tatagctaaa tgggaaaacg tatatgacaa
     1861 agcaaaaggt caagaatatt taaaatcaat ttaaaaaata ctatctgaca tttaaagccc
     1921 cttacaacct ttggttgtta ggggctttta tgcatgcgca aaagcgcagg cgaaccgaag
     1981 cgcagtgacc tcaggttgtt aggggctttt atgcatgcgc aaaagcgcag gcgaaccgaa
     2041 aatagactga cacagtaatt cttaaaaagc agcagtaaga tgattccaaa ttggaaatca
     2101 acttaccgct gcttttactc gtatatacct tgatggcaac taaaatttgt aatttaaatt
     2161 acaattagtg tttcatgtgc attttgccca tgtcatgaat tttcatcatt aaaccgtgag
     2221 ggacgtcatc atggtcaact tttttaactt taacaatttt atctgaatct gctaatttat
     2281 aaacaattac aaaatcacct tttttaactt taacatctga atctgaaggt aatttaacat
     2341 ttttttcaat gctttcatct ttttcactaa tcattttttc aatagtagta ttatctttca
     2401 taattccata gtatgtatct ttctttccgc cagtcatcat catgattact aatacaatac
     2461 atacaattaa catgatggcg attaaactaa taccaagacc aaaacctttt ttctttttca
     2521 tggttcatca atctccttaa atgtagttaa ttctgttata cctaaaagtg caacagtt
//
LOCUS       DAMZKC010000234         2560 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.237, whole genome shotgun sequence.
ACCESSION   DAMZKC010000234 DAMZKC010000000
VERSION     DAMZKC010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.237"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..147
                     /locus_tag="POB55_002528"
     CDS             <1..147
                     /locus_tag="POB55_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HDI6501465.1"
                     /translation="SMERELFEIKETSITHSDGHTSISKTPKVTGKGQQYFVNKFLGE
                     TQTT"
     gene            163..306
                     /locus_tag="POB55_002529"
     CDS             163..306
                     /locus_tag="POB55_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501466.1"
                     /translation="MLQKFRIAKEKNKLKLNLLKHANSNLETSNNPELLRAVAELLKE
                     INR"
     gene            complement(316..969)
                     /locus_tag="POB55_002530"
     CDS             complement(316..969)
                     /locus_tag="POB55_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001803402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501467.1"
                     /translation="MNNINLTQRQLDLIKKNQAILSKLPVEAYAKAANTMNNSYVMNA
                     LEIQSTVNNVMNSIRINQSKLSNWASYMHQVTKNHPMFKSNLFSNEVLNSFIKSTSIP
                     KNDILKMSYALRNLNVDVANSSTFIKSINPAHPVEQKQHESNNYSGKKIVDIMHINHS
                     SLGFINASSVGVSGNAIWDFLLKFINNEPINTPFYISVLFIAYFCYLLTSFSNSNDD"
     gene            1040..1261
                     /locus_tag="POB55_002531"
     CDS             1040..1261
                     /locus_tag="POB55_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501468.1"
                     /translation="MIKNSLQAKELAVILSVSKSKAGQIIRELNKELEDEGYIAIRGR
                     IPVQLARKKFPYHDLSDQRIMEELKKENE"
     gene            1254..1415
                     /locus_tag="POB55_002532"
     CDS             1254..1415
                     /locus_tag="POB55_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1270 family protein"
                     /protein_id="HDI6501469.1"
                     /translation="MSNIYKSYLVAILCFTVLAIVLMPLLYFTTAWSIAGFASIATFI
                     FYKEYFYGE"
     gene            1508..1810
                     /locus_tag="POB55_002533"
     CDS             1508..1810
                     /locus_tag="POB55_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2482 family protein"
                     /protein_id="HDI6501470.1"
                     /translation="MTKNYKDMTQDEIKDLLSEKSGELYELAKEIKGESKFDILLFSS
                     IGVIDGDYLAGSSSVIGHTFDLASLLDSTKSYKDIVNVLQMCKSQKILGIDDDKED"
     gene            1815..2075
                     /locus_tag="POB55_002534"
     CDS             1815..2075
                     /locus_tag="POB55_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1108 family protein"
                     /protein_id="HDI6501471.1"
                     /translation="MYYKTGDVCRKIFNVDGFDFQLRVKKRAYSVEIVVLDHEGNSID
                     GLLVSDENDLYTALDILKQSIYEWIENNTDEQDKLINLIMKW"
     gene            2084..2347
                     /locus_tag="POB55_002535"
     CDS             2084..2347
                     /locus_tag="POB55_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001638663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501472.1"
                     /translation="MRDTERNILNIFKTLFDEYTLSNQRALLEIERNHHGYLSINFLH
                     YHDSYKTNNKLVQIHEINPDSHERIKNLIIEALRGHRKIKKGA"
     gene            2356..>2560
                     /locus_tag="POB55_002536"
     CDS             2356..>2560
                     /locus_tag="POB55_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDI6501473.1"
                     /translation="MKINKLTISNFAGIKEEKFNFDGKDAKIYGNNATGKTTTATALQ
                     WLLFDKGLDGSTKSFNPVPLNEKN"
BASE COUNT         1014 a          376 c          442 g          728 t
ORIGIN      
        1 tcaatggaac gtgagttatt cgaaattaaa gaaacatcaa ttacacattc agacggtcac
       61 acatcaatta gtaagacgcc aaaagtaaca ggcaaaggac aacaatactt tgttaataag
      121 tttttaggtg aaactcaaac aacttaaaag gaggttcaac aaatgttaca aaaatttaga
      181 atcgctaaag aaaaaaataa attaaaactt aatttactaa aacatgcaaa cagtaattta
      241 gaaacaagta acaaccctga actgttgcga gcagttgcag agttgcttaa agagattaat
      301 cgataaatcc gacaactaat catcatttga atttgaaaaa ctggttaata gatagcaaaa
      361 atacgctata aaaagtaccg aaatataaaa aggagtattt attggttcgt tattaataaa
      421 ctttaataaa aagtcccaaa tagcattacc gcttacacct acagaactag cattaataaa
      481 acctaaactg gagtgattaa tatgcattat gtcgacaatt tttttaccgc tgtaattatt
      541 gctttcatgt tgtttttgct ctactggatg ggcaggattg atggatttaa taaaggtaga
      601 actattagct acgtcgacat tcaaatttct aagagcataa gacattttca aaatatcgtt
      661 tttaggaatg ctcgtagatt ttataaaact attaagaacc tcattagaaa ataaattaga
      721 tttgaacatt ggatgattct tagttacttg atgcatatag gaagcccaat tagataattt
      781 cgattggtta attctaatgc tattcataac attattaacc gtcgattgaa tttccagagc
      841 gttcataaca tacgaattat tcatagtatt tgcggcttta gcgtaagctt cgacaggcaa
      901 tttagacaag atagcttgat ttttctttat taaatctaac tgtcgttgag tgagatttat
      961 attattcata attatcacct cctttcacta ggagataact aaattataca caacacaaaa
     1021 ataaaaagga gaaaaagata tgataaaaaa tagtttgcaa gctaaagaac ttgcggtaat
     1081 tttatctgtt tcaaaatcca aagcaggaca aataataaga gaactgaata aagagcttga
     1141 agatgaagga tacattgcga tacgaggcag aataccagtc caattagcta ggaaaaaatt
     1201 cccttatcac gacttatcag accagagaat aatggaggag ttaaaaaaag aaaatgagta
     1261 acatttataa aagctaccta gtagcaatac tatgcttcac agtcttagca attgtgctta
     1321 tgccattgct gtacttcact acagcatggt caatcgcggg attcgcaagt atagcgacat
     1381 tcatatttta taaggaatac ttttatggag aataaaaaaa ctgctacttg cgccaacaag
     1441 taacagtgac aaacacttaa gaaaaatttc aagttaaata taaaacgaaa aacggaggaa
     1501 gtcaaccatg actaaaaact ataaagacat gactcaggac gaaataaaag acttattatc
     1561 tgaaaaaagc ggagaattgt atgaattagc gaaagaaatt aagggagaaa gtaaatttga
     1621 tattttgctt ttctcatcaa taggagttat cgacggagat tatttagcag gttcaagttc
     1681 tgtgattggt catactttcg atcttgcttc cttattggat agcactaaga gttataaaga
     1741 cattgtcaat gttctccaaa tgtgtaaatc acaaaaaatt ctcggtatag atgacgacaa
     1801 ggaggactaa aacaatgtat tacaaaacgg gtgacgtatg tcgaaaaata tttaatgtag
     1861 atggctttga ttttcaatta agagttaaga agcgagcata tagtgtcgaa atagtcgttt
     1921 tagatcatga aggaaattca attgacgggc tactagtttc tgacgagaac gatctataca
     1981 cagctttaga tattttgaaa caaagtattt atgaatggat tgaaaataac acagatgaac
     2041 aggacaaatt aatcaattta atcatgaaat ggtaggtata agcatgagag atacagaaag
     2101 aaatatattg aatattttta agacgttatt cgacgaatat actttgtcaa accaacgagc
     2161 attattggaa attgaacgta atcatcacgg atacttatcg attaatttct tacactatca
     2221 tgacagttac aaaacaaaca ataagcttgt gcagatacat gaaatcaatc cagacagcca
     2281 tgaacgaata aaaaatttaa ttatcgaggc gctaagaggt catcggaaga ttaaaaaagg
     2341 agcatgagga aagatatgaa aataaataag ttaactatat cgaactttgc tggaatcaaa
     2401 gaagaaaaat ttaactttga cggtaaagat gcaaaaatat acggcaataa tgcgactggc
     2461 aagactacaa cagcaaccgc attacaatgg ctgcttttcg ataaaggttt ggacggatca
     2521 accaaatcat ttaaccctgt acctttaaac gaaaaaaacg
//
LOCUS       DAMZKC010000235         2546 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.238, whole genome shotgun sequence.
ACCESSION   DAMZKC010000235 DAMZKC010000000
VERSION     DAMZKC010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.238"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            32..2182
                     /locus_tag="POB55_002537"
     CDS             32..2182
                     /locus_tag="POB55_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI6501474.1"
                     /translation="MAKSCLHILTNNEYATTRCQDGIVLFWPIDGEIELQKFRKSKII
                     EDDIYIINHLDVFSIKNNKKTIMLYLSSDWFAELGFTFFNYHYTAKLIKSSYNLKCLL
                     LKLTYRYLDNQPLNDADIRKLQDIIKIIAKEASMDKKIAQNQYRYAYYGDLRDELEYI
                     YQNVNQRLTLKSVADKLFVSKSNLSSQFHLLMGMGFKKYIDTLKIGKSIEILLTTDST
                     ISNISEHLGFSSSSTYSKMFKSYMDITPNEYRNLSKYNKCLMLKPEPLVGKMVQEVKE
                     IILNYIEHYKNHLTDVIHIDEDKFETPKLFQTVIQINTYTEMKLVFLEGIFKTLLNKN
                     SQVVFFIMPSILKSKNTMSEEEKFTIIKTIIESDLKIAFNINDIETTYFVEEAFMSVF
                     RQISPNELSNHNNYEVHFVFDLSLMEIRTIYRMILKLHNIMLNVKLGLNITCLFEKPS
                     VFKSLVSQIKRLKFDSLIIDNANLSSPYLMGESDELLLKNILHFKNLKQVINELDIEQ
                     EKLIFLNVENHKLLNNKERDLSNSAPLIYKTLSALYHNFDGFGLNIFDNHHTFNAMHL
                     YDKNGFKTTLGLILEKFIEYVSKPKYENSYYSIFDIENYYCLVIYDWRVIESETIMSN
                     FEDSQVYINFKNNVLNDKYLIVIETLDENSGNINHLISKELRDKYEWNPSLLSKIDNY
                     LKPAIEIKEHNFSDNSLNINVTFNALYIIKIGKK"
     gene            2328..>2546
                     /gene="sarX"
                     /locus_tag="POB55_002538"
     CDS             2328..>2546
                     /gene="sarX"
                     /locus_tag="POB55_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001090984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarX"
                     /protein_id="HDI6501475.1"
                     /translation="MNTEKLETLLGFYKQYKALSEYIDKKYKLSLNDLAVLDLTMKHC
                     KDEKVLMQSFLKTAMDELDLSRTKLLVSI"
BASE COUNT         1015 a          295 c          385 g          851 t
ORIGIN      
        1 gtggtactta aatatagaaa gaggtaaagt tatggcaaaa tcatgcttgc atatacttac
       61 taataatgaa tatgcgacaa cgcgttgcca agatggcata gtcttatttt ggccaattga
      121 cggggaaatc gaactacaaa aatttcgtaa aagtaaaata attgaagatg atatatatat
      181 tattaatcat ctggatgtat ttagtattaa gaataataaa aaaacgatca tgttgtattt
      241 gagtagcgat tggtttgcgg aattaggctt tactttcttt aattaccact atacagcaaa
      301 gttgattaaa tcatcctata atttgaaatg tctactatta aaattgacat atcgatacct
      361 tgataatcag cctcttaatg acgctgatat tagaaaatta caggatatta ttaaaatcat
      421 tgcaaaagaa gcaagtatgg ataaaaagat tgcacaaaat caatatcgat atgcttatta
      481 tggtgatttg cgtgatgagc tcgaatatat ttatcaaaat gtaaatcaac gattgacatt
      541 aaaaagtgtc gctgataaat tatttgtctc aaagtcaaat ttgtcatcac aattccactt
      601 acttatgggc atgggtttta aaaaatatat tgatactttg aaaattggta aatcgattga
      661 aattctactt actactgata gtactattag caacataagt gaacatttag gttttagtag
      721 tagctccact tactctaaaa tgtttaaaag ttatatggat ataacaccga atgaatatcg
      781 taatttatca aaatataata aatgtttaat gctaaagcca gaaccactag taggcaaaat
      841 ggtgcaagaa gtaaaagaaa tcatattgaa ttatattgaa cattataaaa accacctaac
      901 tgatgttata catattgatg aagacaaatt tgaaacacct aaattgtttc aaacggttat
      961 tcaaataaat acttatacag aaatgaaatt agttttctta gaaggaatct ttaaaacctt
     1021 attgaataag aacagtcaag ttgtcttttt catcatgcca tcgattctaa aaagtaaaaa
     1081 taccatgtcc gaagaagaaa aattcacaat cattaaaaca ataattgaaa gtgatctaaa
     1141 gatagcattt aatataaatg atattgaaac aacttatttt gttgaagaag cttttatgag
     1201 tgttttcaga caaatatctc caaacgaatt aagtaatcat aataattacg aagtgcattt
     1261 tgtttttgat ttatcattga tggaaattag aacaatttat cgaatgatat taaaattaca
     1321 taacatcatg ttgaatgtga aattaggatt gaacattacc tgtttatttg aaaaaccttc
     1381 agtttttaaa tcactagtat cacaaataaa gcgacttaaa ttcgattcgt taataataga
     1441 taatgcaaat ttaagtagcc cttatttgat gggggaaagt gatgagttac tattgaaaaa
     1501 tattttgcat tttaaaaatt taaaacaagt aattaatgaa ttggatattg aacaagaaaa
     1561 gcttattttt ctaaatgttg aaaatcataa actgcttaat aataaagaac gagatttaag
     1621 taatagtgct ccattaattt ataagacatt aagtgcgctg tatcacaact ttgatggctt
     1681 tggattaaac atttttgata atcatcatac atttaatgcg atgcatctat atgataaaaa
     1741 tggatttaaa acaacactag gtcttatatt ggaaaaattt atcgaatatg tctcgaaacc
     1801 aaaatacgaa aacagttatt attctatttt tgatatagag aattattatt gtcttgttat
     1861 ttatgattgg cgagtgatag agagcgagac aattatgagt aattttgagg atagtcaagt
     1921 ttatataaat tttaaaaaca atgttttaaa cgataaatat ctaattgtaa tagaaacatt
     1981 ggacgaaaat agtggcaaca ttaatcattt gatttctaaa gaattaagag ataaatatga
     2041 atggaaccct agtttactat ctaaaattga caactacctt aaaccagcaa tagagattaa
     2101 agagcataat tttagtgata attccttgaa tattaacgtt acttttaatg cgttatacat
     2161 aattaaaata ggaaaaaaat aacaccttga tatgtattgc aaaatttaat ttgcatttgt
     2221 tggtaatttt gacaacgtat taaaaacaaa tgagaaagac aatggcaaaa aaatttaaga
     2281 taataatgac agaagcattg tctttatata tttgggggtg caacattttg aatactgaga
     2341 aattagaaac attgcttggc ttctataaac aatataaagc attatctgaa tatattgata
     2401 aaaaatataa gttgtcgcta aatgatttag cagtcttaga tttaacgatg aagcattgca
     2461 aagatgaaaa agtacttatg caatcatttt taaaaactgc aatggatgag ctagatttaa
     2521 gtaggacaaa attattagtt tctata
//
LOCUS       DAMZKC010000236         2542 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.239, whole genome shotgun sequence.
ACCESSION   DAMZKC010000236 DAMZKC010000000
VERSION     DAMZKC010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.239"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(<1..498)
                     /locus_tag="POB55_002539"
     CDS             complement(<1..498)
                     /locus_tag="POB55_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HDI6501476.1"
                     /translation="MLCETENINKNPKYRIIKYKDEYLMIDLVSTWLALFFPMINWLI
                     PKKYVKISEKDFETLNIVKTAKINSFWPVAGSTVLFGVMLRRYSHLFIVKYEYSIVIL
                     ICCIIILGIFLFFLYLNQKLKLQIYNENKNKSNKIIIFPTLKSLCLSIVLYIYLGGGS
                     FFTIYM"
     gene            complement(622..1251)
                     /locus_tag="POB55_002540"
     CDS             complement(622..1251)
                     /locus_tag="POB55_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 domain-containing protein"
                     /protein_id="HDI6501477.1"
                     /translation="MLLCDVRVIYKNPKYKVIQHNGEYLLVDLVSTWFVYFFPFINWF
                     IPKKYAIISEEEFENLNVVKPNKNNVFWSVIGSSVLFGVTLRKYIHVFDVQLDKLVVM
                     ILCALALICVIVFYFNLNRKLKLKVFDTNIEKNKRVILIPTFKLGCFLVFGYIFAGSF
                     SIFSLIALMTIEPQNIIIFIYWIMMTMLFFLLNMTSIGNEKVRVIMKNN"
     gene            complement(1704..2333)
                     /locus_tag="POB55_002541"
     CDS             complement(1704..2333)
                     /locus_tag="POB55_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HDI6501478.1"
                     /translation="MLCESRQIYKNPKYRVIRYNNEYFMVDLVSTWITYFFAMINWFL
                     PKKYAKISENEFERLNIVEPVKNNVFWPVAGSSVLFGIILRKYGNFFNVQFEKQLAIT
                     VFFIMLIGMLIFYFYLNKKLTLKIFNTNVVNKNRVVLIPTFKQGLLIVFAYFFLGSAS
                     IFTLTILLTTESQNIIIFLTWVIISMFFFLVNMASIGNKNVHVILRINE"
     gene            complement(2380..>2542)
                     /locus_tag="POB55_002542"
     CDS             complement(2380..>2542)
                     /locus_tag="POB55_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HDI6501479.1"
                     /translation="GFMSYGAFYLLVFENVQNLILYVSWLFMTMLFMFMNMHSIIDKK
                     VHIFLKSNK"
BASE COUNT          960 a          414 c          267 g          901 t
ORIGIN      
        1 catataaata gtaaagaatg aaccaccacc taaataaata tataaaacta ttgataaaca
       61 aagactcttt aaagtgggaa atatgattat cttattgctt ttatttttgt tttcattata
      121 gatttgtaac tttaactttt gatttaaata taaaaaaaac agaaaaatac ctagtattat
      181 gatgcaacaa attaaaatta ctatactata ttcatattta acgataaata aatgggaata
      241 ccttcttaac ataacaccaa ataagaccgt acttcctgcc actggccaaa aagaattgat
      301 tttagctgtc ttcacaatgt ttaaagtttc aaaatctttt tcgctgattt tgacgtactt
      361 ttttggaatc agccaattaa tcattgggaa aaagagtgct aaccatgtac ttactaaatc
      421 aatcatcaaa tattcatctt tgtatttgat aattctatat ttgggattct tattaatatt
      481 ttcagtttca caaagcaatg tctccacttc ttttcaattg gttttatatt gatacacttt
      541 catatacatt agaagtcatt taaattctac tagcgtctta aaaggattgt gtcgacaaca
      601 tttagaatat tttaaatgta atcaattatt tttcattata acgcgaactt tttcattacc
      661 tatcgaagtc atatttaaca aaaagaaaag cattgtcatc ataatccaat aaataaatat
      721 tattatattt tgaggttcga ttgtcataag ggcaattaat gaaaatattg aaaaacttcc
      781 agcgaaaata tatccgaaaa ctaaaaaaca gccaagttta aacgttggta ttaatataac
      841 tctcttattt ttttcaatat ttgtatcaaa cacttttaac ttaagctttc tatttaagtt
      901 aaaataaaaa actataacac aaattaaagc gagagcacac aatatcatta caactagctt
      961 atctaattga acatcaaaaa catgtatgta tttccttaaa gtaactccaa acaaaaccga
     1021 acttcctata actgaccaga aaacattatt tttatttggt ttaacaacat ttaaattttc
     1081 aaattcttct tcgctaatta tcgcgtactt ttttggaatg aaccaattaa tgaaaggaaa
     1141 aaagtacacg aaccaagtgc ttactaaatc gactaataag tattcaccgt tatgttgaat
     1201 gactttgtat ttcggatttt tataaatgac tctgacatcg caaagcaaca tttaccactc
     1261 ccgtttcatt agttttaaat gatccccaat actttttgta ccttcaccaa ctatcgttcc
     1321 aacactatct acagcttttt ctgtggtatt tttaaacagc gtctatttaa tgtcttatcc
     1381 aattagtttt atctcttaat ccaaatttat tagtatgaaa agtccaattt ttaagaattg
     1441 tctaaacaga ttccgcaaca aaaccagcta aaaccgtcca tactattgga ttaaaaacgc
     1501 caatgttata agagctatca tacaaaataa cacgctctat atttcatttt taaattaaaa
     1561 cagttttttc ctttatcaat cacaatgggc attttcactt ggtatattca aacattacta
     1621 tctattttcc tgatttaaga tatgattata tttttttgaa ccaccgttta aatatgcgat
     1681 actaccaatt tcgatcattt ttactactcg tttattctta aaataacatg aacattttta
     1741 ttacctatcg aagccatatt cactaaaaag aaaaacatcg agataataac ccaagttaaa
     1801 aatattatta tattttgtga ctctgtcgtc aaaagaatgg ttaaagtaaa tattgaagcg
     1861 cttcctaaaa aaaagtacgc aaaaactatt aacaatcctt gtttgaaagt cggtattaat
     1921 acaactctat tcttattaac cacgttggta ttaaaaattt ttaatgttaa ttttttattt
     1981 agataaaaat aaaaaattaa catccctatt aacatgataa aaaatacagt gattgctagt
     2041 tgtttttcaa actgaacatt aaagaagtta ccgtactttc tcaatataat tccaaataga
     2101 actgaacttc ctgcaaccgg ccaaaaaaca ttatttttaa caggctcgac tatatttaac
     2161 ctttcaaatt cattttcgct aatttttgcg tattttttgg gcaaaaacca attaatcata
     2221 gcgaaaaaat aagtaatcca agtacttact aaatcgacca tgaaatattc attattatat
     2281 ctaataactc gatatttagg atttttataa atttgtctag attcacaaag cactgtttac
     2341 cacccctaat taacaattga aaactaacta aatttgtaac tatttattag actttaagaa
     2401 tatatgtact tttttatcta taattgaatg catattcata aacataaata gcatagtcat
     2461 gaaaagccaa gaaacatata agattaaatt ttgcacattt tcaaatacca acaagtaaaa
     2521 tgccccataa gacatgaaac ca
//
LOCUS       DAMZKC010000237         2512 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.240, whole genome shotgun sequence.
ACCESSION   DAMZKC010000237 DAMZKC010000000
VERSION     DAMZKC010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.240"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(159..242)
                     /locus_tag="POB55_002543"
     tRNA            complement(159..242)
                     /locus_tag="POB55_002543"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(206..208),aa:Leu,seq:caa)
     gene            complement(371..445)
                     /locus_tag="POB55_002544"
     tRNA            complement(371..445)
                     /locus_tag="POB55_002544"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(410..412),aa:Gly,seq:tcc)
     gene            complement(453..526)
                     /locus_tag="POB55_002545"
     tRNA            complement(453..526)
                     /locus_tag="POB55_002545"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(492..494),aa:Cys,seq:gca)
     gene            complement(532..603)
                     /locus_tag="POB55_002546"
     tRNA            complement(532..603)
                     /locus_tag="POB55_002546"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(569..571),aa:Gln,seq:ttg)
     gene            complement(615..687)
                     /locus_tag="POB55_002547"
     tRNA            complement(615..687)
                     /locus_tag="POB55_002547"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(652..654),aa:His,seq:gtg)
     gene            complement(690..763)
                     /locus_tag="POB55_002548"
     tRNA            complement(690..763)
                     /locus_tag="POB55_002548"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(729..731),aa:Trp,seq:cca)
     gene            complement(779..859)
                     /locus_tag="POB55_002549"
     tRNA            complement(779..859)
                     /locus_tag="POB55_002549"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(823..825),aa:Tyr,seq:gta)
     gene            complement(865..940)
                     /locus_tag="POB55_002550"
     tRNA            complement(865..940)
                     /locus_tag="POB55_002550"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(905..907),aa:Thr,seq:tgt)
     gene            complement(948..1020)
                     /locus_tag="POB55_002551"
     tRNA            complement(948..1020)
                     /locus_tag="POB55_002551"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(985..987),aa:Phe,seq:gaa)
     gene            complement(1039..1114)
                     /locus_tag="POB55_002552"
     tRNA            complement(1039..1114)
                     /locus_tag="POB55_002552"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1079..1081),aa:Asp,seq:gtc)
     gene            complement(1124..1197)
                     /locus_tag="POB55_002553"
     tRNA            complement(1124..1197)
                     /locus_tag="POB55_002553"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1161..1163),aa:Met,seq:cat)
     gene            complement(1212..1304)
                     /locus_tag="POB55_002554"
     tRNA            complement(1212..1304)
                     /locus_tag="POB55_002554"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1266..1268),aa:Ser,seq:tga)
     gene            complement(1343..1418)
                     /locus_tag="POB55_002555"
     tRNA            complement(1343..1418)
                     /locus_tag="POB55_002555"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1383..1385),aa:Asp,seq:gtc)
     gene            complement(1429..1518)
                     /locus_tag="POB55_002556"
     tRNA            complement(1429..1518)
                     /locus_tag="POB55_002556"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1480..1482),aa:Ser,seq:tga)
     gene            complement(1528..1601)
                     /locus_tag="POB55_002557"
     tRNA            complement(1528..1601)
                     /locus_tag="POB55_002557"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1565..1567),aa:Ile,seq:cat)
     gene            complement(1630..1703)
                     /locus_tag="POB55_002558"
     tRNA            complement(1630..1703)
                     /locus_tag="POB55_002558"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1667..1669),aa:Met,seq:cat)
     gene            complement(1725..1800)
                     /locus_tag="POB55_002559"
     tRNA            complement(1725..1800)
                     /locus_tag="POB55_002559"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1765..1767),aa:Ala,seq:tgc)
     gene            complement(1818..1891)
                     /locus_tag="POB55_002560"
     tRNA            complement(1818..1891)
                     /locus_tag="POB55_002560"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1855..1857),aa:Pro,seq:tgg)
     gene            complement(1901..1974)
                     /locus_tag="POB55_002561"
     tRNA            complement(1901..1974)
                     /locus_tag="POB55_002561"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1938..1940),aa:Arg,seq:acg)
     gene            complement(1993..2081)
                     /locus_tag="POB55_002562"
     tRNA            complement(1993..2081)
                     /locus_tag="POB55_002562"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2045..2047),aa:Leu,seq:taa)
     gene            complement(2092..2166)
                     /locus_tag="POB55_002563"
     tRNA            complement(2092..2166)
                     /locus_tag="POB55_002563"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2132..2134),aa:Gly,seq:gcc)
     gene            complement(2170..2251)
                     /locus_tag="POB55_002564"
     tRNA            complement(2170..2251)
                     /locus_tag="POB55_002564"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2215..2217),aa:Leu,seq:tag)
     gene            complement(2255..2330)
                     /locus_tag="POB55_002565"
     tRNA            complement(2255..2330)
                     /locus_tag="POB55_002565"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2295..2297),aa:Lys,seq:ttt)
     gene            complement(2336..2411)
                     /locus_tag="POB55_002566"
     tRNA            complement(2336..2411)
                     /locus_tag="POB55_002566"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2376..2378),aa:Thr,seq:tgt)
     gene            complement(2428..2503)
                     /locus_tag="POB55_002567"
     tRNA            complement(2428..2503)
                     /locus_tag="POB55_002567"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2468..2470),aa:Val,seq:tac)
BASE COUNT          696 a          645 c          589 g          582 t
ORIGIN      
        1 cttccaatat tgaactcatc attacaattt gacatagagt ctattaaagc gtgtgccatt
       61 tgagtccact tttatttgta ttgtatagag agaaataaaa agaaaccttg ttttacaagg
      121 tttctaatac gttatgttat gtaaataaca gttaattata ccggtggtcg gggtcgaacc
      181 gacactccac aagtggaacg ggattttgag tcccgcgcgt ctgccaattc cgccacaccg
      241 gcttaatggt aaacaaaaaa cttccctttg gaagcaatta tggagcggaa gataggattt
      301 acacctatac ctcattccag gaaggaatgt attctaagag ttgaaatact cccgcattat
      361 tattaaatta tggagcggaa gataggattt gcacctatac ctcgttccgg gaaggaacgt
      421 gttctaaaag ttgaactact cccgcataaa cctggaggcg gcaaccggat ttgaaccggt
      481 gataaaggtt ttgcagacct ctgccttacc acttggctat gccgccaata actgggctag
      541 ctggattcga accaacgagt gacggagtca aagtccgttg ccttaccgct tggctatagc
      601 ccattaataa taagggcggc tgaaggggat cgaaccctcg aatgtcggaa ccacaatccg
      661 atgtgttaac cacttcacca cagccgccat ggcaggggca gtaggaatcg aacccacacc
      721 aaaggttttg gagacctcta ttctaccgtt gaactatgcc cctattaaaa ataataaatg
      781 gaggggggca gattcgaact gccgaacccg aaggagcgga tttacagtcc gccgcgttta
      841 gccacttcgc tacccctcca taaatggtgc cggccagagg acttgaaccc ccaacctact
      901 gattacaagt cagttgctct accaattgag ctaggccggc taagaaatgg ttcaggacag
      961 agtcgaactg ccgacacatg gagcttcaat ccattgctct accaactgag ctactgaacc
     1021 ataataaaaa tgtaatgatg gcggtctcga cgggaatcga acccgcgatc tcctgcgtga
     1081 caggcaggcg tgttaaccgc tacactacga gacctataaa atattgcggg aggcggattt
     1141 gaaccaccga ccttcgggtt atgagcccga cgagctaccg aactgctcca tcccgcgata
     1201 ataaaaaata atggcggagg aagagggatt cgaacccccg cggcccgtta aggccctgtc
     1261 ggttttcaag accgatccct tcagccggac ttgggtattc ctccattatt ataggtaaat
     1321 cgctattaat tataaaatta aatggcggtc tcgacgggaa tcgaacccgc gatctcctgc
     1381 gtgacaggca ggcgtgttaa ccgctacact acgagaccat tagtaaaacg gaggaagagg
     1441 gattcgaacc cccgcgagcc gttaagcccc tgtcggtttt caagaccgat cccttcagcc
     1501 ggacttgggt attcctccaa aattatatgg accttgcagg actcgaacct gcgaccgaac
     1561 ggttatgagc cgttagctct aaccaactga gctaaaggtc ctaaatataa ttttacaact
     1621 aataaatagt ggcggtggag gggatcgaac ccccgacctc acgggtatga accgtacgct
     1681 ctagccagct gagctacacc gccttatata gtttgtaaat aatatggtgg agactagcgg
     1741 gatcgaaccg ctgacctcct gcgtgcaaag caggcgctct cccagctgag ctaagccccc
     1801 ataataatta cagtatatcg ggaagacagg attcgaacct gcgacccctt ggtcccaaac
     1861 caagtgctct accaagctga gctacttccc gtataattaa cgcgcccgat aggagtcgaa
     1921 cccataacct cttgatccgt agtcaaacgc tctatccaat tgagctacgg gcgcatatgt
     1981 ttttattgaa aatggtgccg aggaccggaa tcgaaccggt acggtgatca ctcaccgcag
     2041 gattttaagt cctgtgcgtc tgccagttcc gccaccccgg cactataaaa atggagcaga
     2101 agacgggatt cgaacccgcg accccaacct tggcaaggtt gtattctacc gctgaactac
     2161 ttctgcatat gcgggtgaag ggagtcgaac ccccacgccg taaggcgcta gatcctaagt
     2221 ctagtgcgtc tgccaattcc gccacacccg caaatggtga gccatagagg attcgaacct
     2281 ctgaccctct gattaaaagt cagatgctct accaactgag ctaatggctc ttccatggtg
     2341 ccggccagag gacttgaacc cccaacctac tgattacaag tcagttgctc taccaattga
     2401 gctaggccgg caatatgtaa gaataaatgg tggagaatga cgggttcgaa ccgccgaccc
     2461 tctgcttgta aggcagatgc tctcccagct gagctaattc tccgatttaa aa
//
LOCUS       DAMZKC010000238         2470 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.241, whole genome shotgun sequence.
ACCESSION   DAMZKC010000238 DAMZKC010000000
VERSION     DAMZKC010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.241"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            87..809
                     /gene="splB"
                     /locus_tag="POB55_002568"
     CDS             87..809
                     /gene="splB"
                     /locus_tag="POB55_002568"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplB"
                     /protein_id="HDI6501480.1"
                     /translation="MNKNVVIKSLAALTILTSVTGIGITLVEEVQQTAKAENNVTKVK
                     DTNIFPYTGVVAFKSATGFVVGKNTILTNKHVSKNYKVGDRITAHPNSDKGNGGIYSI
                     KKIINYPGKEDVSVIQVEERAIERGPKGFNFNDNVTPFKYAAGAKAGERIKVIGYPHP
                     YKNKYVLYESTGPVMSVEGSSIVYSAHTESGNSGSPVLNSNNELVGIHFASDVKNDDN
                     RNAYGVYFTPEIKKFIAENIDK"
     gene            867..1586
                     /gene="splC"
                     /locus_tag="POB55_002569"
     CDS             867..1586
                     /gene="splC"
                     /locus_tag="POB55_002569"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplC"
                     /protein_id="HDI6501481.1"
                     /translation="MNKNIVIKSMAALAILTSATGINAAVVEETQQIANAEKNVTQVK
                     DTNIFPYNGVVSFKDATGFVIGKNTIITNKHVSKDYKVGDRITAHPDGDKGNGGIYKI
                     KSISDYPGDEDISVMNIEEQAVERGPKGFNFNENVQALNFAKDAKVDDKIKVIGYPLP
                     AQNSFKQFESTGTIKRIKDNILNFDAYIEPGNSGSPVLNSNNEVIGVVYGGIGKIGSE
                     YNGAVYFTPQIKDFIQKHIEQ"
     gene            1707..2426
                     /gene="splF"
                     /locus_tag="POB55_002570"
     CDS             1707..2426
                     /gene="splF"
                     /locus_tag="POB55_002570"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplF"
                     /protein_id="HDI6501482.1"
                     /translation="MNKNIIIKSIAALTILTSITGVGTTVVDGIQQTAKAENTVKQIT
                     NTNVAPYSGVTWMGAGTGFVVGNHTIITNKHVTYHMKVGDEIKAHPNGFYNNGGGLYK
                     VTKIVDYPGKEDIAVVQVEEKSTQPKGRKFKDFTSKFNIASEAKENEPISVIGYPNPN
                     GNKLQMYESTGKVLSVNGNIVSSDAIIQPGSSGSPILNSKHEAIGVIYAGNKPSGEST
                     RGFAVYFSPEIKKFIADNLDK"
BASE COUNT          979 a          356 c          443 g          692 t
ORIGIN      
        1 gtgattaaga aaacggttac ataattaact aaatatactg aattatgtat ctagatatca
       61 aaataattaa aaaagaggaa cttaaaatga acaaaaacgt agtcatcaag agtttagcag
      121 cattaacaat tttaacatct gtaacaggta ttggaataac attggttgag gaagtacaac
      181 aaactgccaa agcagaaaat aatgtcacaa aagttaaaga tacaaatatt tttccatata
      241 ctggtgtagt tgcttttaaa agtgcaactg gatttgtagt tggaaagaat actattttaa
      301 caaataaaca tgtgtcgaaa aattacaaag tgggcgatag aattactgca catccaaata
      361 gtgataaagg taatggtggt atttattcga ttaaaaagat tattaattat ccaggtaaag
      421 aagatgtatc agtcattcaa gttgaagagc gtgcaataga acgtggacca aaaggcttta
      481 attttaatga taatgtaacg ccattcaaat atgcggcagg ggctaaagct ggtgagcgaa
      541 ttaaagtgat tggttatcca cacccataca aaaataaata tgttttatat gagtcaactg
      601 gccctgtgat gtcagtagaa ggtagcagta ttgtatattc agcgcatact gaaagcggaa
      661 actctggatc acctgtatta aacagcaaca acgaattagt tggtattcat tttgcttctg
      721 atgtaaaaaa tgatgataac agaaatgcat atggcgtcta ctttacacca gaaattaaaa
      781 aattcattgc agaaaacata gataaataaa caaattgact ttaaacgagc gttgcaacat
      841 atctcgaatt gtaaaggagc ttgaaaatga ataaaaatat agtcattaaa agcatggcag
      901 cattagccat tctaacctca gcaactggaa taaatgctgc agtcgttgaa gagacacaac
      961 aaatagcaaa tgcagagaag aatgttacgc aagttaaaga tacaaatatt tttccatata
     1021 atggcgtcgt ttcatttaaa gatgcgacag gttttgtaat tggaaaaaat acaattatca
     1081 ccaataaaca tgtatcaaaa gattataaag ttggcgatag aattactgcc catccagacg
     1141 gtgacaaagg aaatggtggt atatataaaa ttaaaagcat ttctgattat ccgggtgatg
     1201 aagacatctc tgtcatgaat attgaagaac aagcagttga acgtggacca aaaggcttta
     1261 attttaatga aaatgtccaa gcattaaatt ttgcgaaaga tgctaaagtt gatgacaaaa
     1321 ttaaagttat tggttaccca ttacctgctc aaaatagttt taaacagttt gaatctacag
     1381 gaactataaa aagaatcaaa gacaatattt taaattttga tgcatacatt gaacccggga
     1441 attcaggatc accagttcta aattctaaca atgaggtcat aggtgtggtg tatggcggta
     1501 ttggaaaaat tggttctgaa tataatggtg ccgtatactt tacgcctcaa atcaaagatt
     1561 ttattcaaaa gcacattgaa caataaacaa atttaaatat acaccatgag catgtgttca
     1621 ataattttaa tgaaaaacat cggtcgaata taacataaaa aaacgtctat atcaaaagca
     1681 tcatgaataa acagaggagc acaaaaatga ataaaaatat aatcatcaaa agtattgcgg
     1741 cattgacgat tttaacatca ataactggtg tcggcacaac agtggttgat ggtattcaac
     1801 aaacagccaa agccgaaaat actgttaaac aaattacaaa tacaaatgtt gcaccataca
     1861 gtggtgttac atggatgggc gctggaacag gatttgtagt tggaaatcat acaatcatta
     1921 ccaataaaca tgttacctat cacatgaaag tcggtgatga aatcaaagca catcctaatg
     1981 gtttttataa taacggtggt ggactttata aagttactaa gattgtagat tatcctggta
     2041 aagaagatat tgcggttgta caagttgaag aaaaatcaac acaaccaaaa ggtagaaaat
     2101 tcaaagattt cactagtaaa tttaatatag catcagaagc taaagaaaat gaacctatat
     2161 cagtcattgg ttatccaaat cctaatggaa ataaactaca aatgtatgaa tcaactggta
     2221 aagtattatc agtgaatggg aatatagtgt catcggatgc aattattcag cctggtagct
     2281 ctggttcacc tatattaaat agtaaacacg aagctattgg tgtaatctat gccggtaata
     2341 agccatcagg tgaaagcaca agaggatttg ctgtttattt ctctcctgaa attaagaaat
     2401 tcattgcaga taatttagat aaataattaa aacttagaca ttcacccaat cctgacaaaa
     2461 tatactataa
//
LOCUS       DAMZKC010000239         2464 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.242, whole genome shotgun sequence.
ACCESSION   DAMZKC010000239 DAMZKC010000000
VERSION     DAMZKC010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.242"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            71..1240
                     /locus_tag="POB55_002571"
     CDS             71..1240
                     /locus_tag="POB55_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NarK/NasA family nitrate transporter"
                     /protein_id="HDI6501483.1"
                     /translation="MYKTKGGFQLTLQTLSLVVGFMAWSIIAPLMPFIKQDVNVTEGQ
                     ISIILAIPVILGSVLRVPFGYLTNIVGAKWVFFTSFIVLLFPIFFLSQAQTPGMLMAS
                     GFFLGVGGAIFSVGVTSVPKYFPKEKVGLANGIYGMGNIGTAVSSFLAPPIAGIIGWQ
                     TTVRSYLIIIALFALIMFIFGDTQERKIKVPLMAQMKTLSKNYKLYYLSYWYFITFGA
                     FVAFGIFLPNYLVNHFGIDKVDAGIRSGVFIALATFLRPIGGILGDKFNAVKVLMIDF
                     VVMIIGAIILGISDHIALFTVGCLTISICAGIGNGLIFKLVPSYFLNEAGSANGIVSM
                     MGGLGGFFPPLVITYVANLTGSSHLAFIFLAVFGCIALFTMRHLYQKEYGSLKNG"
     gene            complement(1347..1775)
                     /locus_tag="POB55_002572"
     CDS             complement(1347..1775)
                     /locus_tag="POB55_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HDI6501484.1"
                     /translation="MILNFNQFENQNFFNGNPSDTFKDLGKQVFNYFSTPSFVTNIYE
                     TDELYYLEAELAGVNKEDISIDFNNNTLTIQATRSAKYKSEQLILDERNFESLMRQFD
                     FEAVDKQHITASFENGLLTITLPKIKPSNETTSSTSIPIS"
     gene            complement(1991..2437)
                     /locus_tag="POB55_002573"
     CDS             complement(1991..2437)
                     /locus_tag="POB55_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDI6501485.1"
                     /translation="MYVENSYLSKQLCFLFYVSSKEIIKKYTNYLKEYDLTYTGYIVL
                     MAIENDEKLNIKKLGERVFLDSGTLTPLLKKLEKKDYVVRTREEKDERNLQISLTEQG
                     KAIKSPLAEISVKVFNEFNISEREASDIINNLRNFVSKNFDYSDKK"
BASE COUNT          783 a          353 c          427 g          901 t
ORIGIN      
        1 tgacctctaa tatataggca gagtattgat atctattaaa aaataaatga ttttggtgaa
       61 ggtgaaacgt atgtacaaaa caaaaggtgg ctttcaactt acattacaga cattaagttt
      121 agtggttggg tttatggctt ggagtataat tgcgccatta atgcccttta ttaaacaaga
      181 tgtcaatgtt actgaaggtc aaatatcaat cattttagcg ataccagtta ttttgggatc
      241 ggtgctccgt gtgccatttg gttatctaac aaacattgtt ggcgctaaat gggtattctt
      301 tactagtttt atcgtattgt tattcccgat atttttctta agccaagcac aaacaccggg
      361 tatgttaatg gcgtcaggat ttttccttgg tgtaggtggt gcaattttct cagttggtgt
      421 tacatcagtt cctaaatatt tccctaaaga aaaagtaggt ctagcaaatg gtatttatgg
      481 tatgggaaat atcggtacag cagtttcttc atttttagca ccgccaattg cgggtattat
      541 tggttggcaa acaactgtta gaagttactt aattattatc gctttatttg cattaattat
      601 gtttatattt ggtgatacac aagaacgtaa aattaaagtg ccattaatgg cacaaatgaa
      661 aacattatct aaaaactaca aattatatta cttaagttat tggtatttca ttacttttgg
      721 tgcttttgta gcatttggta ttttcttacc taactactta gtaaatcatt ttggaattga
      781 caaagtagat gctggtattc gatcaggtgt attcattgcg ctggcaacat tcttaagacc
      841 aataggtggc attttaggtg ataaatttaa tgcagttaaa gtattgatga ttgattttgt
      901 tgttatgatt atcggtgcca ttattttagg tatttcagac catatcgcat tattcactgt
      961 aggctgttta acaataagta tttgtgcagg tattggtaac ggcttaatct tcaaattagt
     1021 accatcctac ttcttaaatg aagcgggatc cgcaaatggt atcgtatcaa tgatgggtgg
     1081 tttaggagga ttcttcccac cactagtaat cacgtacgta gctaatttaa caggatcaag
     1141 tcatttagca tttattttct tagcggtatt tggatgtatt gcattattta ccatgcgtca
     1201 tttataccaa aaagaatatg gctcattgaa aaacggttga tatgtaatac atgacattca
     1261 tttagttaaa tacaaagcct taatatcatg cgcaatattc gtagcatgac attaaggctt
     1321 tagtagtgta ttcattttaa atttaactat gaaattggaa tagatgttga tgaagtagtt
     1381 tcattgcttg gtttgatttt aggcaaggta atggttaata acccattttc aaaactagca
     1441 gtaatatgtt gcttatcaac agcttcaaaa tcaaattgac gcattaatga ttcgaagtta
     1501 cgctcatcta aaatgagttg ttcagatttg tattttgcgc ttctagtagc ttgaatagtg
     1561 agcgtattat tattgaaatc gatactaata tcttctttat ttacacctgc tagttcagct
     1621 tctaagtaat ataattcgtc tgtttcatat atattcgtta caaatgaagg tgttgaaaag
     1681 taattaaata cttgtttacc taaatcttta aatgtatcac ttggattacc gttaaaaaag
     1741 ttttgattct cgaattgatt gaagttcaat atcatcactt ccattataga aatttgtatt
     1801 acaaagccat tatatgagta gtattaaaaa agttaaagta aaaggggaga taaaaaagta
     1861 ataaatgggt agatttgtgc ctgataagga atcagggcaa aagaactgag catcgagatt
     1921 tatacaagca ataaagataa tgaataaaca tagcgatgca tacttctgcc catcacctta
     1981 tgatagggag ttattttttg tcggaatagt caaaattttt agaaacaaaa ttccgcaaat
     2041 tattaattat atctgatgct tctcgttctg aaatgttgaa ttcattaaag accttaacag
     2101 aaatttcagc aagagggctt tttattgctt taccttgttc tgttagagaa atttgtaggt
     2161 ttctttcatc tttctcttca cgtgttcgaa caacgtaatc tttcttttct aatttcttta
     2221 gtaatggtgt cagtgttcca gaatctaaga atacacgttc acctaatttt ttgatgttaa
     2281 gtttttcatc attttcaatc gccattaaaa caatgtaacc agtataggtt aaatcatatt
     2341 ccttaagata gtttgtgtat tttttaatta tttcttttga agaaacataa aataaaaaac
     2401 acaactgttt gctaagatag ctgttttcta catacatcca atcactcctt gttaaaataa
     2461 acaa
//
LOCUS       DAMZKC010000240         2459 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.243, whole genome shotgun sequence.
ACCESSION   DAMZKC010000240 DAMZKC010000000
VERSION     DAMZKC010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.243"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(51..1187)
                     /locus_tag="POB55_002574"
     CDS             complement(51..1187)
                     /locus_tag="POB55_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HDI6501486.1"
                     /translation="MTENKSFKESHPLDDFISDKELSNTTIQKEKLTIEQQKQVDTIS
                     KQINPLDNEGLLAFGSDLQKQMSQFSHQMLDEVQSKDVGPIGDTLSDLISKLKSVNPN
                     ELNTDKPSMLKRIFSRAKSSINEIFSRMQSVSAQVDRITIQLQKHQTHLTRDIELLDT
                     LYDKNKQYFDDLSLHIIAAQQKKLQLENEKLPQLQQQAQQSTNQMDIQHVADMQQFID
                     RLDKRIYDLQLSRQIALQTAPQIRMIQNVNQALAEKIQSSILTSIPLWKNQMAIALTL
                     MRQRNAVAAQRAVTDTTNDLLTANAEMLKQNAIETATENERGIVDLDTLKRTQRNIIE
                     TIEETLIIQQHGREERQLAEKELQQLEQDLKSHLVNIKGTNKQS"
     gene            complement(1219..1848)
                     /locus_tag="POB55_002575"
     CDS             complement(1219..1848)
                     /locus_tag="POB55_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HDI6501487.1"
                     /translation="MTVRYNISHIFGVLVGIPVAFLTSIFGMIALDVSFLIDMSIGIV
                     GFLMTYLPIQKLTSRKYLNEIGLTRKDYRYIRNQLNHTHQKLRGILKTYVNIRSIKDF
                     RQINDIYQISRSIYTTVRQRPASFYKVEGFFYSHIDNALNLVDAYTRLAKMPKKSINE
                     QQKLEQTRITLDEVKRTLIADLKRLNEDDYERLDIEMELNKLHQKHHQD"
     gene            complement(1867..2136)
                     /locus_tag="POB55_002576"
     CDS             complement(1867..2136)
                     /locus_tag="POB55_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HDI6501488.1"
                     /translation="MRHIHLQVFGRVQGVGFRYFTQRIAMNYNIVGTVQNVDDYVEIY
                     AQGDDADIERFIQGVIEGASPASNVTSHQLEELELNQKLSDFRSI"
     gene            2299..>2459
                     /locus_tag="POB55_002577"
     CDS             2299..>2459
                     /locus_tag="POB55_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HDI6501489.1"
                     /translation="MWTVTKIRADYEGWWLFSDWPENIVEKYQYQDFDDMFKHYQQLI
                     NQCKVQFDN"
BASE COUNT          723 a          393 c          385 g          958 t
ORIGIN      
        1 ttaaggaact taatgtccca agctcttttt tgttatatct aattcgtaat ttatgattgt
       61 ttattcgttc ctttgatgtt cactaaatgt gactttaaat cttgttctaa ttgttgtaat
      121 tctttttcag ctaattgtcg ttcttcgcga ccgtgttgtt gaataattaa tgtttcttca
      181 attgtctcaa taatgttacg ctgtgtacgt ttcaatgtat caaggtcaac aatgccacgc
      241 tcattttctg ttgcagtttc aatcgcattt tgtttcaaca tttcagcatt tgctgttaat
      301 aaatcattag ttgtatcagt gacagctcgt tgtgcagcaa ctgcattacg ctgtctcatt
      361 aatgtaagcg caatggccat ttgatttttc catagtggaa tacttgtcaa aattgaactt
      421 tgtatcttct cggcaagtgc ttgattaaca ttttgaatca tacgaatttg tggcgcagtt
      481 tgcaaagcta tttgtcttga aagctgtaag tcatatatgc gtttatctag tctatctata
      541 aattgctgca tatctgcaac atgttgaata tccatttgat tagtggattg ctgcgcttgc
      601 tgttgcaatt gtggtagctt ttcattttct aattgcaact ttttttgctg tgcagcaatg
      661 atatgcaatg ataagtcatc aaagtattgt ttgtttttat catatagcgt atctaataat
      721 tcaatatctc ttgttaaatg tgtttgatgt ttctgcagtt gaatcgttat gcgatcgact
      781 tgagcactaa ctgattgcat tcttgaaaag atttcattga tagacgactt tgctctgcta
      841 aaaattcttt ttaacataga tggtttatca gtatttaact catttggatt aactgacttt
      901 agttttgata ttagatctga caaagtatct ccaataggac caacatcttt actttgtact
      961 tcatccaaca tttgatgtga aaattgagac atttgtttct gtaaatcaga accaaacgct
     1021 aataaacctt cattgtctaa agggttaatt tgtttactga ttgtgtctac ctgtttttgt
     1081 tgttcaattg ttaacttttc tttttgaata gtagtattcg ataattcttt atcgcttata
     1141 aaatcatcta gtggatggct ttctttgaaa cttttatttt cagtcatcaa tcatctctcc
     1201 aatagttgct attttacatc aatcttgatg atgtttttga tgtaacttat ttaattccat
     1261 ttcaatatct aaacgttcat aatcatcttc gttgagacgc tttaaatcag cgattaatgt
     1321 tcgtttgacc tcatccaaag taattcgtgt ttgttctaac ttttgctgtt cattaattga
     1381 ttttttgggc atttttgcta gacgtgtata tgcatcaacc aaatttaaag cattatcaat
     1441 atgagaataa aaaaagcctt caactttata aaatgatgca ggtctctgtc taactgtcgt
     1501 ataaatagaa cgtgaaattt ggtatatatc attaatctgc ctaaaatctt taattgatct
     1561 tatattgaca tacgttttta aaatacctct aagtttttgg tgtgtatgat ttaactgatt
     1621 tcgaatatag cgatagtctt ttctagtcaa accaatttcg tttaaatatt tgcgtgaagt
     1681 gagtttttgt atcggtaggt atgtcattaa aaagccaaca ataccaatag acatatcaat
     1741 taaaaaagat acatcaagtg caatcatccc aaatatgctt gttaaaaacg ctacaggaat
     1801 tcccactaac accccaaata tatgagaaat attatatctc actgtcatct tcctttattt
     1861 agcattttat attgatcgaa aatccgataa tttttgattt agttctaact cttcaagttg
     1921 atggcttgtt acatttgatg ctggtgaggc accttcaatt acaccttgaa taaatctctc
     1981 tatatctgcg tcatcccctt gtgcatatat ctctacatag tcatctacat tttgaacagt
     2041 accgacaatg ttatagttca ttgcaatgcg ttgtgtaaaa tatctaaatc cgacgccttg
     2101 aacgcgtccg aatacttgta aatgtatatg tctcattttt accacctcat aatgttatta
     2161 tacgtagttt tacttaaaaa aactaataat tactatagtt actactttgt ttgtttcaag
     2221 tcgtcaaact tgattttcag aggataaagg tataaaaata agtatagagt ttttgaagta
     2281 tggaaggggt ctttaataat gtggacagtt accaaaatta gagccgatta tgagggatgg
     2341 tggttattca gtgactggcc agaaaacatt gttgaaaaat atcaatatca agattttgat
     2401 gacatgttta agcactatca acaattgatt aatcaatgta aagttcagtt cgataatta
//
LOCUS       DAMZKC010000241         2454 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.244, whole genome shotgun sequence.
ACCESSION   DAMZKC010000241 DAMZKC010000000
VERSION     DAMZKC010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.244"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            163..735
                     /locus_tag="POB55_002578"
     CDS             163..735
                     /locus_tag="POB55_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heptaprenyl diphosphate synthase component 1"
                     /protein_id="HDI6501490.1"
                     /translation="METTVSKLERQIEERLKGVSEYESININHRLGKLLDSYDIPDVA
                     KVACLTIDTSMRHLDDITYNHLSKHSILIGDLISAHFYTLLAEINDLSFQNEISKAIV
                     EINELKSSLHHQALNDYEISQAIVKIETLFPYITLSHFGINIDESEIYNYLFEDMSDY
                     YPSYFKKYNQSEVKHYLHDIQKSYLKSRGN"
     gene            738..1463
                     /locus_tag="POB55_002579"
     CDS             738..1463
                     /locus_tag="POB55_002579"
                     /EC_number="2.1.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="demethylmenaquinone methyltransferase"
                     /protein_id="HDI6501491.1"
                     /translation="MADNKANKEQVHRVFQNISKKYDRLNNIISFEQHKVWRKRVMKD
                     MGVRKGTKALDVCCGTGDWTIALSKAVGPTGEVTGIDFSENMLEVGKEKTASMENVKL
                     VHGDAMELPFEDNSFDYVTIGFGLRNVPDYLVALKEMNRVLKPGGMVVCLETSQPTLP
                     VFKQMYALYFKFVMPIFGKLFAKSKEEYEWLQQSTFNFPGKEELKRMFEEAGFINVRV
                     RSFTGGVAAMHLGYKEKDNTKGD"
     gene            1465..2424
                     /locus_tag="POB55_002580"
     CDS             1465..2424
                     /locus_tag="POB55_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDI6501492.1"
                     /translation="MAKLNMNNEIKKVEQRLEKAIKSKDSVLEQASLHLLSSGGKRVR
                     PAFVILSSQFGKDEQTSEQTYQVAVALELIHMATLVHDDVIDKSDKRRGKLTISKKWD
                     QTTAILTGNFLLALGLEHLMAVKDNRVHQLISESIVDVCRGELFQFQDQFNSQQTIIN
                     YLRRINRKTALLIQISTEVGAITSQSDKETVRKLKMIGHYIGMSFQIIDDVLDFTSTE
                     KKLGKPVGSDLLNGHITLPILLEMRKNPDFKLKIEQLRRDSDRKEFEECIQIIRKSDS
                     INESKAVSSKYLSKALDLISELPDGHPKSLLLSLTKKMGSRNT"
BASE COUNT          920 a          322 c          463 g          749 t
ORIGIN      
        1 gtaaatgaag taaaggttat tatgagaatt acaaaagcta cataaattac tgttagttta
       61 aattgaaatt taaaaatgat aagtaatctt aatgcttcaa aagattttaa tttcttacat
      121 ataatgttaa tatgaatagt gataatatcg tgaggtgtag acatggaaac aactgttagc
      181 aaattggaaa gacaaataga agaaagatta aagggtgtat cagaatatga atccattaat
      241 attaatcatc ggttaggtaa gttacttgat tcatatgata ttccagatgt tgccaaagta
      301 gcatgcctca caattgacac atcaatgaga caccttgatg acattacata taatcatttg
      361 tctaaacatt ccattttgat tggagattta attagtgcac atttttatac gctactagca
      421 gaaatcaatg atttatcatt tcaaaatgaa attagtaaag caattgttga aattaatgaa
      481 ttgaaatctt cattacatca tcaagcttta aatgattatg aaatttctca agcaatagta
      541 aaaattgaaa cacttttccc atatattacg ttatctcatt tcggtatcaa tattgatgag
      601 tcggaaatat ataattattt atttgaagat atgtctgatt actatccatc atacttcaag
      661 aaatataatc aaagtgaagt aaagcattac ttacacgata tccaaaaaag ttatttaaaa
      721 agtagaggta attaagcatg gctgacaata aagcaaataa agagcaagta catcgcgttt
      781 ttcaaaatat ttcaaaaaaa tatgatagat taaataatat tattagtttt gagcagcata
      841 aagtatggag aaaacgcgtc atgaaagaca tgggcgttag aaaagggacg aaagcattag
      901 atgtttgttg tggtactggt gattggacaa tcgcattaag caaagctgta ggaccaacag
      961 gtgaagttac tggtattgac tttagtgaga atatgttaga agtaggaaaa gaaaaaactg
     1021 cttcaatgga aaatgttaaa cttgttcatg gtgatgcaat ggaattgccg tttgaagaca
     1081 attcttttga ttatgtaaca attgggtttg gattaaggaa tgtgccagac tatttagtcg
     1141 cgttaaaaga aatgaataga gtacttaaac caggtggtat ggtggtatgt cttgaaacga
     1201 gccaaccaac tttgccagtc tttaaacaaa tgtatgcatt atactttaaa tttgttatgc
     1261 ctatttttgg gaaattattt gcaaaatcaa aagaagaata tgaatggtta cagcaatcta
     1321 cgtttaattt tcctggaaaa gaagagttga agcgcatgtt tgaagaagcg ggtttcataa
     1381 atgtaagagt acgtagtttt acagggggcg ttgctgcaat gcaccttggc tataaagaaa
     1441 aagataatac caaaggtgat taacgtggca aagttaaaca tgaacaatga aattaagaaa
     1501 gtggaacaac gacttgaaaa ggcaataaaa agtaaagatt ctgtattaga acaggcatca
     1561 ttacacttat tgtcttctgg tggtaaaaga gtacgcccag catttgtaat tctgagtagc
     1621 caatttggca aagatgaaca aacgtctgaa caaacgtatc aagttgcagt cgcattagag
     1681 ttaattcata tggcaacact tgttcatgat gacgttattg ataaaagcga caagcgtcga
     1741 ggcaagttaa ccatatcaaa gaaatgggat cagacaactg ctattttaac tgggaatttt
     1801 ttattggcat taggacttga acacttaatg gccgttaaag ataatcgtgt acatcaattg
     1861 atatctgaat ctatcgttga tgtttgtaga ggggaacttt tccaatttca agaccaattt
     1921 aacagtcaac agacaattat caattattta cgacgtatca atcgcaaaac agcactgtta
     1981 attcaaatat caactgaagt tggtgcaatt acatctcaat ctgataaaga gactgtacga
     2041 aaattgaaaa tgattggtca ttatataggt atgagcttcc aaatcattga tgatgtatta
     2101 gacttcacaa gtaccgaaaa gaaattaggt aagccggtcg gaagtgattt gcttaatggt
     2161 catattacgt taccgatttt attagaaatg cgtaaaaacc cagacttcaa attgaaaatt
     2221 gagcaattac gacgtgacag cgatcgaaaa gaatttgaag aatgtattca aattattaga
     2281 aagtctgaca gtattaatga atctaaagca gtaagttcga agtatttaag taaagctttg
     2341 gatttgattt ctgagttacc agatggacat ccgaaatcac tacttttaag tttgacgaaa
     2401 aaaatgggtt caagaaacac gtagtattta tgaaaagtat tgaaagcgct ttac
//
LOCUS       DAMZKC010000242         2432 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.245, whole genome shotgun sequence.
ACCESSION   DAMZKC010000242 DAMZKC010000000
VERSION     DAMZKC010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.245"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(76..495)
                     /locus_tag="POB55_002581"
     CDS             complement(76..495)
                     /locus_tag="POB55_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002435317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6501493.1"
                     /translation="MFSKVNNQKMLEDCFYIRKKVFVEEQGVPEESEIDEYESESIHL
                     IGYDNGQPVATARIRPINETTVKIERVAVMKSHRGQGMGRMLMQAVESLAKDEGFYVA
                     TMNAQCHAIPFYESLNFKMRGNIFLEEGIEHIEMTKS"
     gene            complement(650..1120)
                     /locus_tag="POB55_002582"
     CDS             complement(650..1120)
                     /locus_tag="POB55_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499548.1"
                     /note="Essential protein for resistance to osmotic stress
                     from salt; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic stress response protein"
                     /protein_id="HDI6501494.1"
                     /translation="MSRKTYEKIANINGMFNMLEQQIIHSQDMAHFRSEFFYVNHEHR
                     ENYEALLIYYKNSIDNPIVDGACYILALPEIFNSVDVFESELPFSWVYDENGITETMK
                     SLSIPLQYLVAAALEVTDVNIFKPSGFTMGMNNWNIAQMRIFWQYTAIIRKEAL"
     gene            complement(1168..2385)
                     /locus_tag="POB55_002583"
     CDS             complement(1168..2385)
                     /locus_tag="POB55_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase"
                     /protein_id="HDI6501495.1"
                     /translation="MNKFLKYFLILLALVLIVVPIVFATLLFKTSQDAFESSQDSKNA
                     NRQSNLRDNKVNPEEQPISILFLGIDDNDGRRKKGQDAEHSRSDAMILTTFNQSKHQI
                     RMLSIPRDTISYIPKVGYYDKITHAHAYGGPIAAMDSVEATMNVPVDYYVRVNMKAFV
                     EAVNELGGIYYDVPYDLNEPNTDDTGKIKIKKGYQKLNGDEALAVARTRHHDSDLKRG
                     QRQMELIKILFQKAQEVDSIDKLDNVIQIVGKNAKHNLTNSEIKALAKMYLTNDVEIK
                     TAQLKGKDDMLNGIYYYHPSVESIQKYANLLRKDLELSPINDKNDFLDQRVINHYGSL
                     IPLTPLDSSLLRKEQNDTTDKDKTSNENNDSTNNSDSSNQQQPATDQNLNQNQGGTQQ
                     APQASNNQNGVVN"
BASE COUNT          749 a          400 c          356 g          927 t
ORIGIN      
        1 aattgatgtc aatttaagaa ttggtttatt acaaatgttt ctaaaataga tacaactttt
       61 tttaattaag cgaggttaac tttttgtcat ttcaatatgc tcgatgcctt cctcaagaaa
      121 tatattacct ctcattttaa agtttaaact ttcataaaat gggatagcat gacattgggc
      181 attcatagta gctacgtaaa aaccttcatc tttagctaat gattctacag cttgcataag
      241 cattctaccc attccttgtc cacgatgtga tttcatcaca gctactcgtt ctattttgac
      301 agttgtttca ttaatagggc gtattcgagc agtggcaact ggctgtccat tatcatatcc
      361 aatgaggtga atagattcag attcatattc atcaatttca ctttcctcag ggacgccttg
      421 ttcttctaca aacactttct ttcttatata gaagcaatct tctaacattt tttgattgtt
      481 tacttttgaa aacataaaat tcctccacat cctaacaaaa tcatttacat ttaatttact
      541 aataataaca tagaataacg attgttattt tcctcaaaaa taattagaca taaaaaagct
      601 ataaagatga ttgttgtgaa tcatctttat agctaattaa ttattaatgt tatagtgctt
      661 cttttctaat aattgctgta tattgccaaa agattcgcat ttgagcaata ttccaattat
      721 tcattcccat tgtaaatcct gaaggcttaa atatattcac atcagttact tctaaagctg
      781 ctgcaactaa atattgtaat ggaatgctaa gtgatttcat tgtttcggta atgccatttt
      841 catcatatac ccatgaaaat ggtaactctg attcgaaaac atcaacacta ttgaaaattt
      901 caggtagggc taaaatataa catgcaccat ctacaatagg attgtcgata ctatttttgt
      961 aataaattag gagtgcttca tagttttctc gatgctcatg attgacgtaa aaaaattcac
     1021 ttctaaaatg agccatatct tggctatgaa tgatttgttg ttctaacata ttaaacatgc
     1081 catttatatt tgcaatcttt tcgtatgttt ttctactcat gtatgatgct cctttcataa
     1141 atagtaaatg ttaatcgtca tataatatta atttacaaca ccattttggt tatttgaagc
     1201 ttgtggcgct tgttgtgtgc caccttgatt ttgatttaag ttttgatctg tagcaggttg
     1261 ttgttgattg ctggaatcac tgttattagt tgaatcattg ttctcgttag atgtcttatc
     1321 tttatctgtc gtatcatttt gttcttttct caataaacta ctatctaaag gcgttaatgg
     1381 tattaatgaa ccataatgat taatgacacg ttgatctaag aaatcatttt tatcattaat
     1441 aggtgataat tctaagtctt tacgaagtaa gtttgcatat ttttgaatgc tttcaacact
     1501 tggatgataa tagtaaatac catttaacat atcatcttta ccttttaatt gcgcagtttt
     1561 aatttcaaca tcatttgtta agtacatttt tgctaaagct ttaatttcag agttagttaa
     1621 attatgcttt gcatttttac ctacaatttg aatcacgtta tcaagtttat caatagaatc
     1681 aacttcctgt gctttttgga ataaaatctt aattaattcc atttgacgtt gtccacgttt
     1741 taagtctgaa tcatgatgtc tagttctagc aactgctaaa gcctcatcac catttaattt
     1801 ttggtaccct tttttaattt taatcttacc agtatcatct gtgttaggtt catttaagtc
     1861 gtatggcaca tcatagtata tgccaccaag ctcatttaca gcctcgacaa atgctttcat
     1921 attgactctc acataataat caacaggtac attcatggta gcttctaccg aatccattgc
     1981 ggcaattgga ccaccatatg catgtgcatg ggtaatctta tcgtaatagc caactttagg
     2041 aatgtagctg atagtatcac gtggaatact aagcattcta atttgatgtt ttgattgatt
     2101 aaaagtagtt aaaatcatag cgtctgatct agagtgttca gcatcctgtc ctttttttct
     2161 tcttccatcg ttatcatcga tacctaagaa aagaatagag ataggttgtt cttcgggatt
     2221 gactttatta tctcttaagt tggattgacg attagcattt ttgctgtctt gagaagattc
     2281 gaatgcatct tgggacgttt taaaaagtaa cgtagcgaag actattggaa caacaatgag
     2341 aaccaatgct agaaggatca aaaagtattt taaaaattta ttcatgattg atgctcctat
     2401 attaaatttt tgttaaatca taatcataat tt
//
LOCUS       DAMZKC010000243         2425 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.246, whole genome shotgun sequence.
ACCESSION   DAMZKC010000243 DAMZKC010000000
VERSION     DAMZKC010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.246"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            101..874
                     /gene="sea"
                     /locus_tag="POB55_002584"
     CDS             101..874
                     /gene="sea"
                     /locus_tag="POB55_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal enterotoxin type A"
                     /protein_id="HDI6501496.1"
                     /translation="MKKTAFILLLFIALTWTTSPLVNGSEKSEEINEKDLRKKSELQG
                     AALGNLKQIYYYNEKAKTENKESHDQFLQHTILFKGFFTNHSWYNDLLVDFDSKDIVD
                     KYKGKKVDLYGAYYGYQCAGGTPNKTACMYGGVTLHDNNRLTEEKKVPINLWLDGKQN
                     TVPLETVKTNKKNVTVQELDLQARRYLQEKYNLYNSDVFDGKVQRGLIVFHTSTEPSV
                     NYDLFGAQGQNSNTLLRIYRDNKTINSENMHIDIYLYTS"
     gene            983..1159
                     /locus_tag="POB55_002585"
     CDS             983..1159
                     /locus_tag="POB55_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HDI6501497.1"
                     /translation="MDRWWLSEYKEVVPMVALLKSLERRRLMITISTMLQFGLFLIAL
                     IGLVIKLIELSNKK"
     gene            complement(1212..1319)
                     /locus_tag="POB55_002586"
     CDS             complement(1212..1319)
                     /locus_tag="POB55_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501498.1"
                     /translation="MIFILWCVNLYIEKGQHAQTCYPNEPVKKTVAILD"
     gene            1371..1625
                     /locus_tag="POB55_002587"
     CDS             1371..1625
                     /locus_tag="POB55_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HDI6501499.1"
                     /translation="MINWKIRMKQKSFWVAILSAIFLFAQNIAKAIGYDIQVYTEQLT
                     DGLNAILGFLVLTGVIQDPTTKGIGDSHQALEYEEPRRKY"
     gene            1637..2392
                     /locus_tag="POB55_002588"
     CDS             1637..2392
                     /locus_tag="POB55_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDI6501500.1"
                     /translation="MKTYSEARARLRWYQGRYIDFDGWYGYQCADLAVDYIYWLLEIR
                     MWGNAKDAINNDFKNMATVYENTPSFVPQIGDVAVFTKGIYKQYGHIGLVFNGGNTNQ
                     FLILEQNYDGNANTPAKLRWDNYYGCTHFIRPKYKSEGLMNKITNKVKPPAQKAVGKS
                     ASKITVGSKAPYNLKWSKGAYFNAKIDGLGATSATRYGDNRTNYRFDVGQAVYAPGTL
                     IYVFEIIDGWCRIYWNNHNEWIWHERLIVKEVF"
BASE COUNT          886 a          309 c          471 g          759 t
ORIGIN      
        1 ttgtatataa ctaataatta cttcgacaaa aataatctat tatccaaata ttttagataa
       61 taaaaagttt gtatggaatt atgctttaga ggtgagcaaa atgaaaaaaa cagcatttat
      121 actactttta ttcattgccc taacgtggac aacaagtcca cttgtaaatg gtagcgagaa
      181 aagcgaagaa ataaatgaaa aagatttgcg aaaaaagtct gaattgcagg gagcagcttt
      241 aggcaatctt aaacaaatct attattacaa tgaaaaagct aaaactgaaa ataaagagag
      301 tcacgatcaa tttttacagc atactatatt gtttaaaggc ttttttacaa atcattcatg
      361 gtataacgat ttattagtag attttgattc aaaggatatt gttgataaat ataaagggaa
      421 aaaagtagac ttatatggtg cttattatgg ttatcaatgt gcgggtggta caccaaacaa
      481 aacagcttgc atgtatggtg gtgtaacgtt acatgataat aatcgattga ccgaagagaa
      541 aaaagtgcca atcaatttat ggctagacgg taaacaaaat acagtacctt tggaaacggt
      601 taaaacgaat aagaaaaatg taactgttca ggagttggat cttcaagcaa gacgttattt
      661 acaggaaaaa tataatttat ataactctga tgtttttgat gggaaggttc agaggggatt
      721 aatcgtgttt catacttcta cagaaccttc ggttaattac gatttatttg gtgctcaagg
      781 acagaattca aatacactat taagaatata tagagataat aaaacgatta actctgaaaa
      841 catgcatatt gatatatatt tatatacaag ttaaacatgg tagttttgaa cacgtaatgt
      901 tcagattatt atgaaccgag aataatctga aagtttacaa gcagtaaaaa aagtatatgt
      961 gctataatat gctttgagca agttggatag atggtggcta tctgagtata aggaggtggt
     1021 gcctatggtg gcattactga aatctttaga aaggagacgc ctaatgatta caattagtac
     1081 catgttgcag tttggtttat tccttattgc attgataggt ctagtaatca agcttattga
     1141 attaagcaat aaaaaataac catcgctaac tttggctggt ttcgatggtt aaatggttat
     1201 taatttaatc tttaatctaa aatagccacc gtctttttaa cgggctcatt agggtaacat
     1261 gtttgcgcat gttgcccttt ttctatatat aaattaacac accataatat aaatatcaaa
     1321 tagacggctt attagtcgtc tttttatttt ggataaaagg agataagaat atgattaatt
     1381 ggaaaattag aatgaaacaa aaatcatttt gggtagcgat attgtcagct atctttttat
     1441 ttgctcaaaa catcgcaaaa gctattgggt atgatatcca agtttataca gagcaattaa
     1501 cagacggttt aaacgctata ttaggatttt tagtattaac tggtgtgatt caagacccga
     1561 ctactaaagg tataggtgat agccaccaag ctttagaata tgaagaacca agaagaaaat
     1621 actaggaggt aaaataatga aaacatacag tgaagcaaga gcaaggttac gttggtatca
     1681 aggtagatat attgattttg acggttggta tggttaccaa tgtgcagatt tagcagttga
     1741 ttacatttat tggttgttag aaattagaat gtggggaaat gcaaaagatg caatcaataa
     1801 cgattttaaa aacatggcaa cagtatatga aaacacacca tcgtttgttc cacaaatagg
     1861 tgatgtggct gtatttacca aaggaatata taaacaatac ggtcatattg gtttagtgtt
     1921 taatggtggt aatacaaatc aatttttaat tttggaacag aactatgacg gtaacgcaaa
     1981 tacgcctgca aagttacgtt gggataatta ttacggctgt actcacttta ttagacctaa
     2041 gtataaaagt gagggcttaa tgaataagat cacaaataaa gttaaaccac ctgctcaaaa
     2101 agcagtcggt aaatctgcaa gtaaaataac agttggaagt aaagcgcctt ataaccttaa
     2161 atggtcaaaa ggtgcttatt ttaatgcgaa aatcgacggc ttaggtgcta cttcagccac
     2221 tagatacggt gataatcgta ctaactatag attcgatgtt ggacaggctg tatacgcgcc
     2281 tggaacatta atatatgtgt ttgaaattat agatggttgg tgtcgcattt attggaacaa
     2341 tcataatgag tggatatggc atgagagatt gattgtgaaa gaagtgtttt aattcttagg
     2401 ttaaaatgtt aaatatttgt taatt
//
LOCUS       DAMZKC010000244         2382 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.247, whole genome shotgun sequence.
ACCESSION   DAMZKC010000244 DAMZKC010000000
VERSION     DAMZKC010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.247"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..725
                     /gene="seh"
                     /locus_tag="POB55_002589"
     CDS             <1..725
                     /gene="seh"
                     /locus_tag="POB55_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="staphylococcal enterotoxin type H"
                     /protein_id="HDI6501501.1"
                     /translation="INKIKILFSFLALLLSFTSYAKAEDLHDKSELTDLALANAYGQY
                     NHPFIKENIKSDEISGEKDLIFRNQGDSGNDLRVKFATADLAQKFKNKNVDIYGASFY
                     YKCEKISENISECLYGGTTLNSEKLAQERVIGANVWVDGIQKETELIRTNKKNVTLQE
                     LDIKIRKILSDKYKIYYKDSEISKGLIEFDMKTPRDYSFDIYDLKGENDYEIDKIYED
                     NKTLKSDDISHIDVNLYTKKKV"
     gene            743..1480
                     /locus_tag="POB55_002590"
                     /pseudo
     CDS             743..1480
                     /locus_tag="POB55_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032492129.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exotoxin"
     gene            1621..2094
                     /locus_tag="POB55_002591"
     CDS             1621..2094
                     /locus_tag="POB55_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6501502.1"
                     /translation="MEYKSYNKMSNNILSILLSDKNFDNKMVVSTGSYILSELPCIVA
                     YKNNDIAGLLTYKVYDEYIEIISLDSFVENKGIGSHLLDYAEIIASDMSKRSISVITT
                     NENIKALYFYQKNKYRITDVIFDAVTEARKIKPSIPTIDKNGIEIRDEIVLTKYL"
     gene            complement(2199..2382)
                     /locus_tag="POB55_002592"
                     /pseudo
     CDS             complement(2199..2382)
                     /locus_tag="POB55_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662030.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          941 a          282 c          384 g          775 t
ORIGIN      
        1 tgattaataa aattaaaata ttattttcgt ttttagcatt attactttca ttcacatcat
       61 atgcgaaagc agaagattta cacgataaaa gtgagttaac agatttagct ttagctaatg
      121 catatggtca atataatcac ccattcatta aagaaaatat taagagtgat gaaataagtg
      181 gagaaaaaga tttaatattt agaaatcaag gtgatagtgg caatgatttg agagtaaagt
      241 ttgcaactgc tgatttagct cagaagttta aaaataaaaa tgtagatata tatggggcat
      301 ctttttatta taagtgtgaa aaaataagtg aaaatatttc tgaatgtcta tatggaggta
      361 caacactaaa tagtgaaaaa ttggcacagg aaagggtgat tggtgctaat gtttgggtag
      421 atggtattca aaaagaaaca gaattaatac gaacaaataa gaaaaatgtg acattgcaag
      481 aattagatat aaagatcaga aaaatattgt ccgataaata taaaatttat tataaagaca
      541 gcgaaataag taaaggtcta attgaatttg atatgaaaac tcctagagat tactcattcg
      601 acatttatga tttaaaagga gaaaatgact atgagataga taaaatttat gaagacaata
      661 aaactttaaa atctgatgat ataagtcata ttgatgtaaa tctatatact aagaaaaaag
      721 tataatcaat gaaaatttct agatcaatgt tagtgttaat tgtcatgtct tttttccgtt
      781 atggaattca tttaagcatt tttctcaaac aagtacatat actccaaaag cagaaactct
      841 aaagaacaag tccgacttag atttaaccgc gttatataat attaaacctg tatattcaga
      901 aagtgaattt cttaaatttt aaagataaaa ttaaaaccaa gcaactatta tataattatt
      961 taatgttata attaaataat tgaataaaga tagtctaaaa aaagagctta atagttcgga
     1021 aattgcaaac acattcaaag gaaaaatgta gatatattcg gagtacactt tggagcaaat
     1081 tgtgttggag aagcaggtga gaatagcgct tgtttatatg gtggagtcac atttcacgaa
     1141 aaaatgaaaa acaacaaaaa agtcaatagg cataaatgta tttaaaaatg gatttcaaca
     1201 aaaagggttt gtcattacta ctgctaaaat caaacccaca gctcaagagc tagatcttaa
     1261 aacgagaaac aatatacaaa atcaatataa aatgtatgat tcggaaactg gagatataca
     1321 aaaaggatat ataaaatttc attctactaa atcaagtttc tattatgact tatttgattt
     1381 taaggtgaaa aaacgtgtgg atttcttaaa gttctataat gacaatgaac ttataagtac
     1441 caaaaaatta catatagata tctatttgtt taacaagtaa ataattggaa acgtttattg
     1501 ataccttaca gccatatggg cagacaaaat gtcagtttat cttttcatca gttgtgtaaa
     1561 ttactttgca aaaacttagt aagtgtggga aagattatta acagagagaa gtgacttatt
     1621 ttggaatata aatcttacaa caaaatgagt aataatattt tatctatctt gttgagtgat
     1681 aaaaatttcg ataataaaat ggtagtctca acaggtagtt acattttgtc tgagttgcca
     1741 tgtattgttg cctataaaaa taatgatatc gcggggctac taacgtataa agtttatgat
     1801 gagtatattg aaatcatttc tttagatagt tttgtagaga acaagggtat tggtagtcat
     1861 ttgcttgatt atgctgaaat aattgcctca gatatgagta aaagaagtat cagtgttatt
     1921 actacaaatg aaaatattaa ggcactttat ttctatcaaa aaaataaata tagaatcaca
     1981 gacgttattt ttgatgctgt aacagaagct aggaaaataa agccgtcgat tcctacaatt
     2041 gataaaaatg gcattgaaat tagagatgaa attgttttga caaaatattt gtaatatata
     2101 aatatacgtt gttttaaatc accctagttc ataccaaaaa gactttaaca caaacaaaaa
     2161 aggaggaaac ttaaattcct ccttgaccct catttcatct ataattctat tttaacgtct
     2221 tcgtccattt gggcttcaaa ttcatctagt agtgctcgtg cttctgcaat tgattgtgtg
     2281 ttcatcaatg ccatattttt ctgcaagttc aagtcctgtt tttctatcgg gaatatcacc
     2341 gttaattgtt aacaatgtat ttggtgcaat ttcgtcacgt aa
//
LOCUS       DAMZKC010000245         2371 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.248, whole genome shotgun sequence.
ACCESSION   DAMZKC010000245 DAMZKC010000000
VERSION     DAMZKC010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.248"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            51..239
                     /locus_tag="POB55_002593"
     CDS             51..239
                     /locus_tag="POB55_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501503.1"
                     /translation="MAQYAKHYEQKSDSSNAHTLNSSSAIIEQHTMSNLASLDLYAPV
                     RNITSSRDIHPIYFMTKD"
     gene            383..691
                     /locus_tag="POB55_002594"
     CDS             383..691
                     /locus_tag="POB55_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501504.1"
                     /translation="MTVNIVFSIVFCISMVILGIYVAITKDFTLISFINQTTIADKHK
                     NQIAYIFTLCISLSAVFLMSSILSFEYDFIALAFLFLTIALLLIALFYVCFYKITKYP
                     "
     gene            complement(799..1032)
                     /locus_tag="POB55_002595"
     CDS             complement(799..1032)
                     /locus_tag="POB55_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501505.1"
                     /translation="MLRSLCKHNRILINAIKVGIEMKYKISLAYNLAIIIGSLIILCI
                     LISRGYDIYVILIPILTILASLINLFCDIKKHK"
     gene            complement(1013..2206)
                     /locus_tag="POB55_002596"
     CDS             complement(1013..2206)
                     /locus_tag="POB55_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6501506.1"
                     /translation="MLYLEVLKNRNFTYLLIGNFLRRSCFVLFSLQIIWFTVELTNQS
                     SLKLSMMVMSQTLPFIIFGIFGGAYSDKHNKKKILYLSDLILSLIIIIIPLLAITSNL
                     NYLTLLTISTAITIINCYTDPAFRAILPEIIDEEHLATSNALIDSLQRGSNIILPALI
                     GVIVILVGNVGVFFICSILLFLGFIFNALLKYTNISMIDRHSKEDFSETWEFLKKSKE
                     IPFIIIIQFACILINTGLWRVALPLFISNILKEGVGVYGLATSCLGIASLLMSLIMGL
                     LSEKRLIFKFSIGVLVWGIGLSIINVFPSVAILYIGATLVGLGQSIEGLTRSVAIQIK
                     TPNHLMGKVFSISSTSNYAADTLSLGLISLIIPLLSLSNIFISGGAIISMLSLAGLRF
                     VKKSM"
BASE COUNT          870 a          411 c          292 g          798 t
ORIGIN      
        1 ttatgggcat tatatctctt attgttttat ttatttttac actattcatc atggcgcaat
       61 atgcaaaaca ttatgaacaa aaatccgaca gttccaacgc acatacttta aattcatcat
      121 cagccataat tgagcaacat actatgtcga atttagcgtc tctagattta tacgctcctg
      181 ttcgtaacat tacttctagt cgtgatattc atcccattta tttcatgaca aaagattaaa
      241 acttcctttt tcataacccc attcattcaa ctaataaatt aaaaattgat aaaaacgtac
      301 tgttttttag aaacatacat atataagaaa cgcatccatt actaaaagac agtacattat
      361 attctatcgg gaaggtgcaa caatgaccgt caacatcgtg ttttctatcg tgttctgtat
      421 cagtatggtt atattaggca tttatgtcgc aataactaaa gatttcacac taatctcttt
      481 tataaatcaa acgaccattg cagataaaca caagaaccaa attgcatata tatttacgct
      541 atgtatcagt ttgagtgcag tatttttgat gtcttcaatt ctaagctttg aatatgattt
      601 tattgcatta gcattcttat ttttaacgat agcattatta cttatcgcat tattctacgt
      661 ttgcttttat aaaattacaa aatacccgta aatgtttgtg atagtgtgtt ggttagattt
      721 attgatgcgc tgtcagtaag taattgtttg cgaaaaaaat acaaccttgt acgaatacaa
      781 agttgcatta atatagaatc atttatgctt tttaatatca caaaataaat tgattaaact
      841 agctaaaata gtcaaaattg gaatcaagat tacataaata tcataacctc tagatattag
      901 tatacataat ataattagcg accctattat aattgctaaa ttataagcca aactaatttt
      961 atatttcatt tcaattccca ccttaatagc attgattaaa attctattat gtttacatag
     1021 acttcttaac aaaccttaac ccagctaagc taagcattga aataattgca cctcctgata
     1081 taaatatatt acttaagctt agtaaaggta taattaaact tataagtcct aaagacaatg
     1141 tatccgctgc gtaattcgat gtagatgaga tactaaatac tttccccatc aaatgatttg
     1201 gcgtctttat ttgaatggca actgatcttg ttagtccctc tatagattgc ccaagtccca
     1261 ctaaagttgc acctatatat agtatcgcca cacttggaaa cacattaatt atcgataagc
     1321 caatccccca aactaaaaca ccaatactaa atttaaagat taatcgtttt tctgacagta
     1381 aacccataat caatgacatt aataaagacg ctatacctaa acatgatgta gctagtccat
     1441 atacaccaac gccctctttt aatatattgg aaataaacaa tggtaatgca acacgccaaa
     1501 gaccagtatt aatcaatatg caagcaaatt ggatgatgat gataaatgga atttctttag
     1561 attttttcaa gaattcccaa gtttcagaaa aatcttcttt tgagtgtcta tcaatcatgc
     1621 tgatatttgt atattttaaa agtgcattaa aaataaatcc taaaaatagc aatatactgc
     1681 aaataaaaaa gacgccaaca ttaccaacta gtattacaat gacaccaatt aaagcaggta
     1741 aaataatatt tgagcctctt tgcaaactat cgattaacgc attacttgtt gctaaatgct
     1801 cctcatcaat aatttcagga agaattgccc taaacgcagg atccgtatag cagttaataa
     1861 tggtgatagc tgtagatatg gttagaagcg tcagataatt taaatttgat gttattgcaa
     1921 gtaaaggaat aattattata atcaaactta gtataagatc agatagataa agtattttct
     1981 ttttattatg tttatcagaa tatgcgccac cgaaaatacc aaatataata aatggtagtg
     2041 tttgactcat aaccatcatt gataatttta aagatgattg gtttgtcaat tcaacagtaa
     2101 accaaattat ttgtaacgaa aacagcacaa agcaactccg acgtaagaaa ttaccaatca
     2161 ataaatatgt aaagtttcta tttttcaaaa cttctaagta caacatattt atcacctctc
     2221 ataaaaataa ttgaatgcat ccaccagctt ttttagacct tcttctaaac tctctttatc
     2281 caaagcgcaa ttaattctaa tataatttag tcagttaaat atcaattatt tcgaaattta
     2341 catactactt gaaacaccat acataacccc c
//
LOCUS       DAMZKC010000246         2321 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.249, whole genome shotgun sequence.
ACCESSION   DAMZKC010000246 DAMZKC010000000
VERSION     DAMZKC010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.249"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..451
                     /locus_tag="POB55_002597"
     CDS             <1..451
                     /locus_tag="POB55_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HDI6501507.1"
                     /translation="QKGEQFPIFKLENQDGTVITNDTLKGKKAIIYFYPRDNTPTCTI
                     EACDFRDNLEMFNDLDVAVYGISGDSKKKHQNFIEKHGLNFDLLVDEDFKLAKETGVY
                     QLKKSFGKESMGIVRTTFIIDEQGKVLDVIEKVKVKTQIEELKNILG"
     gene            457..1407
                     /locus_tag="POB55_002598"
     CDS             457..1407
                     /locus_tag="POB55_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDI6501508.1"
                     /translation="MKVVGLNRMREVETELQQRFSDLDFKFYKKASEIPESDLADLDI
                     LVGYDGGINEAFLQRCPNLKWIAWFATGVNTLPLDYIADHGILLTNGKGVQAKQLSEY
                     ILAFILDDYKKMKLSYDNQRQHIYDSKITGKRLSGQTVLFLGTGAIATRTAKLAKAFN
                     MNLIGLSKSGQNKDEFDEIYTIESLESTLPNADIIINALPETQETIHLLKKKHFELMK
                     DEALFINIGRGSIVKEALLIEVLKSKVIRHAYLDVFENEPLKPNHELYELDNVTITAH
                     ITGNDYEAKYDLLDIFKNNLVNFLNKNDLIENEVDAKKGY"
     gene            1504..1950
                     /gene="perR"
                     /locus_tag="POB55_002599"
     CDS             1504..1950
                     /gene="perR"
                     /locus_tag="POB55_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide-responsive transcriptional repressor
                     PerR"
                     /protein_id="HDI6501509.1"
                     /translation="MSVEIESIEHELEESIASLRQAGVRITPQRQAILRYLISSHTHP
                     TADEIYQALSPDFPNISVATIYNNLRVFKDIGIVKELTYGDSSSRFDFNTHNHYHIIC
                     EQCGKIVDFQYPQLNEIERLAQHMTDFDVTHHRMEIYGVCKECQDK"
BASE COUNT          923 a          283 c          393 g          722 t
ORIGIN      
        1 gcaaaaagga gaacaatttc caatatttaa attagaaaat caagacggaa ctgtcattac
       61 aaatgataca ttaaaaggta aaaaggcgat tatatatttt tatcctagag ataatacacc
      121 tacttgtacc atagaagctt gtgactttag agacaattta gaaatgttca atgatttaga
      181 tgttgcagta tatggtataa gcggtgattc aaagaaaaaa caccaaaatt ttattgagaa
      241 acacggattg aatttcgatt tattagtaga tgaagatttt aaattagcta aagaaactgg
      301 cgtatatcag ttaaaaaaat catttggcaa agaaagtatg ggcattgtaa gaacgacttt
      361 tataatagat gaacaaggta aagtattaga tgttatcgag aaggttaagg ttaaaacaca
      421 aatagaagaa cttaaaaaca ttttggggtg acatatatga aagttgttgg gttgaatcgt
      481 atgcgtgaag ttgaaactga attacaacaa cgcttttcag atttagattt taaattttat
      541 aaaaaagcat cagaaatacc tgagagcgac ttggctgatt tagatatatt agttggttat
      601 gatggcggta tcaatgaggc atttttacaa cgttgcccga atttaaaatg gattgcatgg
      661 tttgcaacgg gtgtaaatac attgccgtta gattatattg cagatcacgg catactttta
      721 actaatggaa aaggtgttca agctaaacaa ttatctgaat acattttagc tttcatttta
      781 gatgattata aaaagatgaa actatcatat gataaccaac gacaacatat atatgattcg
      841 aaaataactg gtaaacgcct atcaggacaa acagttttat ttttaggtac aggtgcaatt
      901 gctactagaa ctgcgaagtt agcaaaggct tttaatatga atttaattgg tctgagcaag
      961 tcaggtcaga acaaagatga gtttgacgag atatatacta tcgaatcact tgaaagcaca
     1021 ttaccaaatg ctgacattat tataaatgct ttaccagaaa cgcaagaaac gattcattta
     1081 ctaaagaaaa aacattttga attaatgaaa gatgaagcac tttttataaa tataggacga
     1141 ggtagcatag ttaaagaagc gctcttaata gaagtattaa aaagtaaagt tattcgacat
     1201 gcatatttag atgtgtttga aaatgaacct ttgaaaccta atcatgaatt atatgaattg
     1261 gataatgtaa ctataacagc gcatataact ggtaatgatt atgaggcaaa gtatgactta
     1321 ttagatattt ttaaaaacaa tctagttaat tttctcaata agaatgatct aattgagaat
     1381 gaagttgatg ctaaaaaagg ctattaaatg aaatcatcat gtaaatattg acacgcgcgc
     1441 aatactacag ttatatttat agtaagtaat aataattatt atataagaaa gatggtgata
     1501 tagatgagtg ttgaaataga atcaattgaa catgaactag aagaatcaat tgcatcattg
     1561 cgacaagcag gcgtaagaat tacacctcaa agacaagcaa tattacgtta tttaatttct
     1621 tcacatactc atccaacagc tgatgaaatt tatcaagcac tttcacctga ttttccaaat
     1681 ataagtgttg cgacaatata taataactta agagtgttta aagatattgg aattgtaaaa
     1741 gaattaacat atggagactc atcaagtcga ttcgacttta atacacataa tcattatcat
     1801 attatatgtg aacaatgtgg taagattgtt gattttcaat atccacagtt aaatgaaatt
     1861 gaaagattag ctcagcatat gactgacttt gacgtaacac atcatcgaat ggaaatttat
     1921 ggagtttgta aagaatgcca agataaataa tttaactttg gtagtatgac aaattaaaaa
     1981 ggcgttagct tacttcatat aagtaagcgt aatatttaag aagttaaacg acatgaaagt
     2041 tgtttaactt cttttttatg cttattctga aaaatattta aatagaagaa aagaacattc
     2101 taactacgga agtggtatta actaataact agtatccttt ataaataaac ataaaaatat
     2161 tcaaacgaag gtctactaaa taactatcca taaagtgagt ataagtttgt ttgtaaaact
     2221 gaagtaaaaa gcgtaaggaa ttacacttta aatacatata gtacttacga atagaaaata
     2281 atcccttcaa cagtaatttt taaacaaaaa taagtgttgt t
//
LOCUS       DAMZKC010000247         2318 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.250, whole genome shotgun sequence.
ACCESSION   DAMZKC010000247 DAMZKC010000000
VERSION     DAMZKC010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.250"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(<1..271)
                     /locus_tag="POB55_002600"
     CDS             complement(<1..271)
                     /locus_tag="POB55_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDI6501510.1"
                     /translation="MKKYDIAVLDFETMNEHMNSPCEVAVSLIKDLSIVKVYSSYINP
                     PNNRYNLKNAKIHKIPEDVILKAPKYPDIYQEILYLLKESHLIIAH"
     gene            complement(287..1000)
                     /locus_tag="POB55_002601"
     CDS             complement(287..1000)
                     /locus_tag="POB55_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI6501511.1"
                     /translation="MNKERNIIIAKNIRKFLNDSNMSQKKLAELINIKPSTLSDYLNL
                     RSNPSHGVIQRIADVFEVGKSDIDTTYKDDNDITSIYNKLTPPRQENVLNYANEQLEE
                     QNSKGDNVVDINSYKQEKTPVNVNGCVSAGVGERLHDETLFTEMVKGPIPTHDLALKV
                     NGDSMEPMFKDGEIIFVEKTHNIKNGQIGIFIIEEEAYVKKVFVEDDRLTLVSLNKDY
                     DDLHFYRNESVRLIGKVIL"
     gene            963..1136
                     /locus_tag="POB55_002602"
     CDS             963..1136
                     /locus_tag="POB55_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDI6501512.1"
                     /translation="MFLAIIIFLSLFILLTSFFLIIRNFHIMVKFFTKKTYLMLTIRK
                     FRIILGLRKAVTT"
     gene            1133..1396
                     /locus_tag="POB55_002603"
     CDS             1133..1396
                     /locus_tag="POB55_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI6501513.1"
                     /translation="MKTLKELRTDYGLTQKELGDLFKVSSRTIQNMEKDSTNIKDSLL
                     SKYMSAFNVKYDDIFLGNEYENFVFKNDKKKSIILAFKEKQTS"
     gene            1412..1627
                     /locus_tag="POB55_002604"
     CDS             1412..1627
                     /locus_tag="POB55_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HDI6501514.1"
                     /translation="MNIQVATKLAMEKGISIRRENQDVYGILPTNLQRYQCLVVSRHY
                     KKKRQTAAGRWQPSADDLIADDWILDY"
     gene            complement(1616..1945)
                     /locus_tag="POB55_002605"
     CDS             complement(1616..1945)
                     /locus_tag="POB55_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501515.1"
                     /translation="MTDEAKFVLLQLYSIYLDRIDEGMSKRSASYFGSDESSFNAFFL
                     GFNFEDYIDAVLELKHRDFVIASAEDGGFLEMTLSREGIAYSESESKKDYKTLMGLIR
                     DLKKLII"
     gene            1996..>2318
                     /locus_tag="POB55_002606"
     CDS             1996..>2318
                     /locus_tag="POB55_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HDI6501516.1"
                     /translation="MQALQTKSNIGEMFNIQEKENGEIAISGRELHQALEVKTPYKKW
                     FERMSDYGFEENIDYIVTDIFVHNPLGGRQNQTDHALTLDTAKEIAMIQRSEPGKRAR
                     QYFIQV"
BASE COUNT          805 a          363 c          392 g          758 t
ORIGIN      
        1 tatgagcaat aattaaatgt gattctttta aaagatagag aatttcttgg taaatatctg
       61 gatatttagg tgcttttaat atgacatctt caggtatttt atgtatttta gcgtttttca
      121 agttatatct attattagga ggattaatat aagatgaata aacttttact attgataaat
      181 ccttaattaa agatacagca acttcgcaag ggctgttcat atgttcattc atagtttcaa
      241 agtctaagac tgcaatatca tattttttca tttgcaagtg ctccttttat aaaataactt
      301 ttccaattaa cctcacactt tcatttctat aaaagtgtag atcgtcgtaa tctttattta
      361 gtgaaactag agtcaatcta tcatcttcaa caaagacttt cttaacgtac gcttcttctt
      421 caatgatgaa tataccaatt tgtccattct ttatattgtg agttttctcc acaaatatga
      481 tttcgccatc tttaaacata ggttccatag aatcaccatt tacttttaac gctaaatcgt
      541 gtgtggggat aggtccttta accatttcag taaatagcgt ttcatcgtgt aaacgttctc
      601 ctacaccagc agagacgcaa ccattgacgt taactggagt tttctcctgt ttatatgaat
      661 taatatctac aacgttatct cctttagaat tctgttcttc caattgttca tttgcatagt
      721 taagtacgtt ttcttggcgg ggaggtgtga gtttgttgta tatggaagtg atgtcgttat
      781 cgtctttgta tgtagtatct atgtcgcttt taccaacctc gaaaacatca gctatccttt
      841 gtataacgcc gtgagagggg ttggaacgta aatttaaata atcgcttaaa gtagatggtt
      901 ttatgttaat gagttcagca agtttctttt gagacatatt tgaatcgttg agaaattttc
      961 taatgttttt ggctataata atatttcttt ctttgttcat attacttacc tccttttttc
     1021 ttattatacg aaattttcat atcatggtaa agttttttac gaaaaaaacg tatttaatgt
     1081 tgacaatacg aaaatttcgt attatattag gtttacgaaa ggcggtgaca acatgaaaac
     1141 attaaaagag ttgaggactg attacggatt gactcaaaaa gagttaggag atttatttaa
     1201 ggtctcatca cgtacaattc aaaatatgga aaaagactct acaaacatta aagatagttt
     1261 actttctaag tatatgagtg cttttaatgt taaatatgat gatatttttt taggtaatga
     1321 atacgaaaat ttcgtattta agaatgataa aaagaaatca attattttag catttaaaga
     1381 aaaacaaaca tcttaatagg aggaataaca aatgaacatt caagtagcaa cgaagctagc
     1441 gatggagaaa ggaataagta taaggagaga gaatcaagat gtgtatggga tattaccaac
     1501 taatttgcag cgttatcaat gcctagtcgt atctagacac tataagaaaa aaagacaaac
     1561 cgccgccgga aggtggcagc ctagcgcaga cgatttaata gcagatgatt ggattttaga
     1621 ttattaattt tttcaaatct ctaattaaac ccataagtgt tttgtaatct tttttggatt
     1681 ctgattctga gtaggcgata ccttctcgag aaagagtcat ctcaagaaaa ccgccatctt
     1741 cagcagaagc aattacaaaa tctctatgct ttaattcaag aactgcatcg atatagtctt
     1801 caaaattaaa acctaaaaag aaagcgttaa atgaggattc atcactaccg aaataagatg
     1861 cagaacgttt agacatacct tcgtcaattc tatcaaggta aattgaataa agttgtaaaa
     1921 ggacaaattt agcttcatca gtcataagtc attcacctcc ttaataggag tatagcagaa
     1981 aggagcacaa acaatatgca agcattacaa acaaaatcga acataggcga aatgttcaac
     2041 atacaagaaa aagaaaatgg agaaatcgca atcagcggtc gagaacttca tcaagcatta
     2101 gaggttaaga ctccatacaa aaaatggttt gaaagaatga gtgattacgg atttgaagaa
     2161 aatatcgatt atatagtcac ggacattttt gtccataacc cactaggagg tcgtcagaat
     2221 caaactgacc acgcactcac actagacact gcaaaagaga ttgcaatgat tcaacgtagt
     2281 gaacctggca aacgtgcaag acaatacttc atccaagt
//
LOCUS       DAMZKC010000248         2317 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.251, whole genome shotgun sequence.
ACCESSION   DAMZKC010000248 DAMZKC010000000
VERSION     DAMZKC010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.251"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(157..444)
                     /locus_tag="POB55_002607"
     CDS             complement(157..444)
                     /locus_tag="POB55_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501517.1"
                     /translation="MTNEDKRFEQLRFERKFIVIPYLIYAVIVLLLNIFYSDLKITMT
                     LFGLFFAYNVVILFIAFIKHYKRTLLLSLILTVLSGAAFFGIIYVYGINHF"
     gene            complement(479..1387)
                     /locus_tag="POB55_002608"
     CDS             complement(479..1387)
                     /locus_tag="POB55_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HDI6501518.1"
                     /translation="MEQIMINHYVHFSRLVQGFWRANEWKMTAKELNYFINELVERGI
                     TTMDHADIYGDYQCESLFGNALDLSPELRNKIQIVTKCGIILPSKQFDFTNGHRYDLS
                     SKHIVKSVEQSLINLNVDYLDSLLIHRSSPLMDPEQVADALTKLVKQGKLKSFGVSNF
                     NHSQYQLLNQYIMKERLHISINQLELSPYHVDSLQDGTMDSMYQNHVQIMAWSPFAGG
                     KIFDKEDIKAQRIMKVVQSIADKYGVSDTAVMIAWLVKIPHRIMPILGTSQLKRIDQA
                     IEGLQLNLDDQSWFDIYTAIIGQDIP"
     gene            complement(1490..>2317)
                     /locus_tag="POB55_002609"
     CDS             complement(1490..>2317)
                     /locus_tag="POB55_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDI6501519.1"
                     /translation="LKNDEKIKIIMNEFGTFDIDSNSISNEIEVHSLINGCVCCDLKQ
                     ELVYELKAIALKGDVNHVIIETTGIAHPLELLVACQDPQIVNFFEKPIIYGVLDATRF
                     LERHQYTENTVSLMEDQLKLSDMIIINKIDLITDDRLERIDKQLGMICASIPTYKTTY
                     GKVSLEELDLTVKDREISSHHHHHHGIKSMTYTFTGPIDRQLFYQFIMKLPESVLRLK
                     GYVSFRDQPNAIYEFQYAYGLPDYGIIGMQLPLTIVIIGETLDTNHIRNQLDMLQFT"
BASE COUNT          802 a          407 c          314 g          794 t
ORIGIN      
        1 atctcggtta attacaaaac gatatctaag tttaaatatt agatcataaa aaaactatct
       61 actgaatatc ctcatagcat catttttcta agcaaaagtt aaacttagtt agataaaatg
      121 caaatgagat tattgtagat agtctctttt taatatttaa aaatgattaa tgccataaac
      181 ataaataatt ccaaagaatg ccgcgccact aagcactgtt aatataagac ttagtaacaa
      241 tgtgcgttta taatgtttaa taaatgctat gaacaaaatg actacattat acgcaaagaa
      301 aagtccgaat aatgtcattg ttattttcaa atcagaatag aaaatattta atagtaatac
      361 aatgactgca taaattaaat acggaataac tataaatttg cgttcaaatc ttaattgttc
      421 gaaacgttta tcttcgtttg tcatgatatg ctcctttatg atttaaaagt aaataagttt
      481 acggaatatc ttgtccgata atagcggtgt aaatgtcaaa ccacgactga tcatctaaat
      541 taagttgtag cccttcgatt gcttgatcaa tacgctttaa ctgacttgtt ccaagtatcg
      601 gcatgatacg atgcggtatt tttactaacc atgctatcat cacagctgtg tcactcacac
      661 catatttgtc agctattgat tgaacaactt tcataatacg ttgcgcttta atatcttcct
      721 tgtcgaaaat tttaccgcct gcaaaaggac tccaagccat aatttgaaca tggttttgat
      781 acattgaatc cattgttcca tcttgtaaac tatcaacgtg atatggcgat aattctaatt
      841 gattgatgct aatatgtagt ctttctttca taatatattg atttaacaat tggtattgtg
      901 aatgattaaa attcgacacc ccgaatgact tcaacttacc ttgtttaaca agtttagtta
      961 atgcatcagc aacttgttct ggatccatca atggtgaaga acgatgaatg agtagactat
     1021 ctaaataatc tacattcaaa ttgattaatg actgttcaac agatttcacg atgtgcttac
     1081 tactcaaatc ataacgatgt ccatttgtaa aatcaaattg cttagaaggc aaaatgatac
     1141 cacatttcgt aacaatttga attttatttc ttaattcggg tgataaatcc aaagcattac
     1201 caaacagtga ttcacattga taatccccat aaatatcagc atgatccatc gttgtaattc
     1261 cacgttcaac taattcattt ataaaataat ttaactcttt cgcagtcatc ttccattcat
     1321 ttgcacgcca aaaaccttgt acaagcctag aaaaatgaac atagtgatta atcattattt
     1381 gttccatatc tcatccactc catccctcaa tgtcacaaaa tattcacaat aaaagtatta
     1441 agaaattaat gtgctatttc cgttaaattg aaacaacaca ttttctgatt tacgtaaatt
     1501 gtagcatatc caattgatta cgtatgtgat ttgtatctaa agtttcacca ataataacaa
     1561 tcgttaatgg taattgcatg ccaattattc catagtctgg taaaccatat gcatattgaa
     1621 attcataaat tgcatttggt tgatctctaa atgacacata acctttcaaa cgtagaacag
     1681 attccggtaa tttcattata aattgataaa acaattgacg atcaatcgga cctgtaaacg
     1741 tgtaagtcat acttttaatc ccatgatgat ggtgatgatg agacgatatc tctctgtctt
     1801 taacagttaa gtccaattct tccaacgaaa cttttccata ggttgtttta tatgttggaa
     1861 tacttgcaca aatcatacct aattgcttat caattctctc aagacggtca tcagttataa
     1921 gatcaatttt attaataata atcatgtcac ttagtttcaa ctgatcttcc atcagcgaaa
     1981 ctgtattttc ggtatattga tgacgttcta aaaatcgagt cgcatctaat acaccataaa
     2041 taatcggctt ttcaaagaaa ttaacgattt gcggatcttg acatgcaact agtaattcca
     2101 aaggatgcgc aatgcctgtc gtttctatga tgacatgatt aacgtcccct tttaaagcaa
     2161 tggcttttag ttcatagaca agttcttgtt taagatcgca acaaacacaa ccattaatca
     2221 atgaatggac ttcaatttca tttgaaatac tattgctatc aatatcaaaa gtaccgaatt
     2281 cattcatgat gattttaatt ttctcatcat tttttaa
//
LOCUS       DAMZKC010000249         2310 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.252, whole genome shotgun sequence.
ACCESSION   DAMZKC010000249 DAMZKC010000000
VERSION     DAMZKC010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.252"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            35..>2310
                     /gene="cna"
                     /locus_tag="POB55_002610"
     CDS             35..>2310
                     /gene="cna"
                     /locus_tag="POB55_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001039417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen adhesin Cna"
                     /protein_id="HDI6501520.1"
                     /translation="MNKNVLKFMVFIMLLNIITPLFNKNEALAARDISSTNVTDLTVS
                     PTKIEDGGKTTVKMTFDDKSVKIQNSDTIKVAWPTSGTVKIEGYSKTVPLTVKGEQVG
                     QAVITPDGATITFNDKVEKLSDVSGFAEFEVQGRNLTQTNTSDDKVATITSGNKSTNV
                     TVHKSEAGTSSVFYYKTGDMLPEDTTHVRWFLNINNEKRYVSKDITIKDQIQGGQQLD
                     LSTLNINVTGTHSNYYSGSNAITDFEKAFPGSKITVDNTKNTIDVTIPQGYGSYNSFS
                     INYKTKITNEQQKEFVNNSQAWYQEHGKEEVNGKSFNHTVHNINANAGIEGTVKGELK
                     VLKQDKDTKAPIANVKFKLSKKDGSVVKDNQKEIEIITDANGIANIKALPSGDYILKE
                     IEAPAPYTFDKDKEYPFTMKDTDNQGYFTTIENAKEIEKTKDVSAQKVWEGTQKVKPT
                     IYFKLYKQDDNQNTTPVDKAEIKKLEDGTTKVTWSNLPENDKNGKTIKYLVKEVNAQG
                     EDTTPEGYTKKENGLVVTNTEKPIETTSISGEKVWDDKDNQDGKRPEKVSVNLLANGE
                     KVKTLDVTSETNWKYEFKNLPKYDEGKKIEYTVTEDHVKDYTTDINGTTITNKYTPGE
                     TSATVTKNWDDNNNQDGKRPTEIKVELYQDGKATGKTATLNESNNWTHTWTGLDEKAK
                     GQQVKYTVEELTKVKGYTTHVDNNDMGNLIVTNKYTPETTSISGEKVWDDKDNQDGKR
                     PEKVSVNLLANGEKVKAV"
BASE COUNT          995 a          310 c          466 g          539 t
ORIGIN      
        1 atggatatga catagagtat aaggaggggt ttttatgaac aagaacgtgt tgaagtttat
       61 ggtctttata atgttattaa atatcatcac gcctttattt aataaaaatg aagcattggc
      121 agcacgagat atttcatcaa cgaatgttac agatttaact gtatcaccga ctaagataga
      181 agatggtggt aaaacgacag taaaaatgac attcgacgat aaaagtgtaa aaatacaaaa
      241 tagtgacacg attaaagtgg catggccgac aagcggtaca gtaaagatag agggttatag
      301 taaaacagta ccattaactg ttaaaggtga acaggtgggt caagcagtta ttacaccaga
      361 cggagcaaca attacattca atgataaagt agaaaaatta agtgatgttt cgggatttgc
      421 agaatttgaa gtacaaggaa gaaatttaac gcaaacaaat acttcagatg acaaagtagc
      481 tacgataaca tctgggaata aatcaacgaa tgttacggtt cataaaagtg aagcgggaac
      541 aagtagtgtt ttctattata aaacgggaga tatgctgcca gaagatacga cacatgtacg
      601 atggttttta aatattaata atgaaaaaag atatgtatcg aaagatatta ctataaagga
      661 tcagattcaa ggtggacagc agttagattt aagcacatta aacattaatg tgacaggtac
      721 acatagcaat tattatagtg gatcaaatgc aattactgat tttgaaaaag cttttccagg
      781 ttctaagata actgttgata atacgaagaa cacaattgat gtaacaattc cacaaggcta
      841 tgggtcatat aatagttttt caattaacta caaaaccaaa attacgaatg aacagcaaaa
      901 agagtttgtt aataattcac aagcttggta tcaagagcat ggtaaggaag aagtgaacgg
      961 gaaatcattt aatcatactg tgcacaatat taatgctaat gccggtattg aaggtactgt
     1021 aaaaggtgaa ttaaaagttt taaaacagga taaagatacc aaggctccta tagctaatgt
     1081 aaaatttaaa ctttctaaaa aagatggatc agttgtaaag gacaatcaaa aagaaattga
     1141 gattataaca gatgcaaacg gtattgctaa tattaaagcg ttgcctagtg gagactatat
     1201 tttaaaagaa atagaggcgc cagcaccgta tacatttgat aaggataaag aatatccgtt
     1261 tactatgaaa gatacagata atcagggata ttttacgact attgaaaatg caaaagagat
     1321 agaaaaaaca aaagatgtat ctgctcaaaa ggtttgggaa ggcactcaaa aagtgaaacc
     1381 aacgatttat ttcaagttgt acaaacaaga tgacaatcaa aacacaacac cagtagacaa
     1441 agcagagatt aaaaaattag aagatggaac gacaaaagtg acatggtcta atcttccaga
     1501 aaatgacaaa aatggcaaga ctattaaata tttagttaaa gaagtaaatg ctcaaggtga
     1561 agacacaaca ccagaaggat atactaaaaa agaaaatggt ttagtggtta ctaatactga
     1621 aaaaccaatc gaaacaacat caattagcgg tgaaaaagta tgggacgaca aagacaatca
     1681 agatggtaag agaccagaaa aagtcagtgt gaatttattg gctaatggag agaaagtaaa
     1741 aacgttagac gtgacatctg aaacaaactg gaagtacgaa tttaaaaact taccgaagta
     1801 tgatgaagga aagaaaatag aatatacagt gaccgaagat cacgtaaaag actacacaac
     1861 agacatcaac ggtacgacaa taacgaacaa gtatacacca ggagagacat cggcaacagt
     1921 aacaaaaaat tgggatgaca ataataacca agacggaaaa cgaccaactg aaattaaagt
     1981 tgagttatat caagatggaa aagcaacagg aaaaacggca acattaaatg aatctaataa
     2041 ctggacccat acgtggacag gattagatga aaaagcaaaa ggtcaacaag taaaatacac
     2101 agtcgaggaa ttaacaaaag ttaaaggcta tacaacacat gtggataaca atgatatggg
     2161 taacttgatt gtgacgaata aatatacgcc agaaacaaca tcaattagcg gtgaaaaagt
     2221 atgggacgac aaagacaatc aagatggtaa gagaccagaa aaagtcagtg tgaatttatt
     2281 agctaacgga gagaaagtaa aagcggtaga
//
LOCUS       DAMZKC010000250         2284 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.253, whole genome shotgun sequence.
ACCESSION   DAMZKC010000250 DAMZKC010000000
VERSION     DAMZKC010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.253"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            267..641
                     /locus_tag="POB55_002611"
     CDS             267..641
                     /locus_tag="POB55_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4467 domain-containing protein"
                     /protein_id="HDI6501521.1"
                     /translation="MKRILVVFLMLAIILAGCSNKGEKYQKDIDKVYKEQNQMNKIAS
                     KVQNTIKTDIKQEDSNTHVYKDGKVIVIGIQLYKDREKMYYFAYEIKDGKAEINREID
                     PIKYMKDHKADYEDENVEVEKD"
     gene            791..1189
                     /locus_tag="POB55_002612"
     CDS             791..1189
                     /locus_tag="POB55_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HDI6501522.1"
                     /translation="MEKSIKIMTIIGIVIQGLATVFSLLLMVLAASGVMTTDVSTTVN
                     GEVDPVDAETAAAIFTVLFLFLFIFGIISIILGAIGMFKASKNKKMSGILLIIGAVIS
                     GNIITFALWLVSGIKLLTNNKPKDEISDLS"
     gene            complement(1439..2263)
                     /locus_tag="POB55_002613"
     CDS             complement(1439..2263)
                     /locus_tag="POB55_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDI6501523.1"
                     /translation="MKEKHIKWDKIFYGDDWVNNVVETIRTKDILQSVYLKRYLLRAM
                     MAGFIIGIITVFVLSVKATHEPDLPPGIVNMASAITFSFALVLILFTNSELLTSNFMY
                     FTVGLYYKVIKPTRVLKIFLLCFAGNILGAAILFSFMRFSNVMTPDMLNQLSAVIEHK
                     TLSTGFVSILMKAIFANFFINISLVIAMQIDDVLAKMFVMMFGVTIFAFMGYEHVVYN
                     SCLFMGGLIYQVDTLHFIPAISNIAAAFIGNYIGGGLIIGLFYAYLNDHHQFYKNN"
BASE COUNT          885 a          317 c          361 g          721 t
ORIGIN      
        1 acatagcaat ataaaggtat caaatagtta ttgcataatt tattatgatg acataatttt
       61 cagtagatag ttgtaaatta acactgaaat agatgtttat attaagaaga ctttaatatc
      121 ataattaatc aaaatcttgc gtcaaacatt taaacgaatt ttaagttgat aacaattggc
      181 aaaacattgt tcaaacatca caatgataaa gcatattatc agtattgtag tgtgtggaaa
      241 atgacagcca tctaaggaga aaaatgatga aaagaatatt ggtagtattt ttaatgttag
      301 caattatatt ggcaggttgt tctaataaag gtgaaaagta tcaaaaagat attgataaag
      361 tgtacaaaga acagaatcaa atgaataaaa ttgcctcgaa agtacaaaac actattaaaa
      421 cagacattaa acaagaagac agtaatacac atgtttataa agatggtaaa gtcattgtta
      481 ttggtattca attatataaa gatcgtgaaa aaatgtatta tttcgcatat gaaataaaag
      541 atggtaaggc agagattaat agagaaatag acccaattaa gtatatgaaa gaccataaag
      601 cagattatga agatgaaaat gtagaagtgg aaaaagatta acataggctt tctttaaaac
      661 ttttaacctg tttcaaaaag taaatgagct ttagcgacaa taatttttaa aataaacacg
      721 atataaattt aaacatttat atataagaac acaaacgtaa gtttgtaaat aataataaag
      781 gagtaagtca atggaaaaat cgatcaaaat aatgacaata ataggaattg ttattcaggg
      841 tttagcaacg gtatttagtt tactattgat ggttttagca gcatcaggtg taatgactac
      901 agatgtgtca acaacagtta atggtgaggt tgacccagtt gatgcagaaa cagcagcagc
      961 aattttcact gtattattct tattcctatt catatttgga atcatttcaa ttattttagg
     1021 tgcaatcggt atgtttaaag catctaaaaa caaaaaaatg agtggtatat tgttgattat
     1081 tggagctgta ataagtggta acataattac atttgcttta tggttagtca gtggtattaa
     1141 actacttact aataacaagc ctaaagatga aataagcgac ttatcataaa catcgtatat
     1201 tgaaatttca agatttctta agtaattata tttctgttag tttttaataa attaaccgat
     1261 taaataaagt gctctcttag aagtagattt tcagtcaata actgctttta agagagcatt
     1321 ttgtatttga taatgctcat agcatcggac aaaaaccatt gacgaacttt aaaaaaatac
     1381 gttagacaat gcacttctaa atattaaagg catcgactaa cgcattgttc aaatagagtt
     1441 aattattttt ataaaattga tgatgatcat tcaagtaagc atagaataaa cctataatga
     1501 gtcctcctcc aatatagtta ccgataaaag ccgcagcgat attcgaaata gctggtatga
     1561 agtgcaatgt atcaacttga taaattaaac cacccataaa taagcaactg ttgtaaacga
     1621 cgtgttcata acccataaag gcgaatatgg taacaccgaa catcatgaca aacatttttg
     1681 cgagtacatc gtcaatttgc atggcaataa ctaatgaaat attgataaag aaattggcga
     1741 atatcgcttt cattaatata cttacaaaac cagtagacaa cgttttgtgc tcaataactg
     1801 ctgataactg atttaacata tctggcgtca ttacatttga aaaacgcatg aaactaaata
     1861 aaatggcagc acctaaaata tttcctgcaa agcacaataa aaatattttc aatactctag
     1921 tcggtttaat tactttataa tacaggccta cagtaaagta catgaagtta ctggttagta
     1981 gttcggagtt tgtaaataaa atgagtacta acgcaaagct gaatgtaatg gcactggcca
     2041 tattcacaat gcctggcggt aaatctggtt cgtgtgttgc tttaactgat aatacgaaga
     2101 ccgtaataat cccgataata aatcctgcca tcatcgcgcg taataaataa cgttttaaat
     2161 aaacgctttg taatatatct tttgttctta ttgtttcgac tacgttattt acccagtcgt
     2221 ccccataaaa tattttatcc catttaatat gtttctcctt cacaatgaca tcctcacttt
     2281 tcat
//
LOCUS       DAMZKC010000251         2275 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.254, whole genome shotgun sequence.
ACCESSION   DAMZKC010000251 DAMZKC010000000
VERSION     DAMZKC010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.254"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            210..1769
                     /gene="rny"
                     /locus_tag="POB55_002614"
     CDS             210..1769
                     /gene="rny"
                     /locus_tag="POB55_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002459258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HDI6501524.1"
                     /translation="MNLLSLLLILLGIILGVVGGYVVARNLLLQKQSQARQTAEDIVN
                     QAHKEADNIKKEKLLEAKEENQILREQTEAELRERRSELQRQETRLLQKEENLERKSD
                     LLDKKDEILEQKESKIEEKQQQVDAKESSVQTLIMKHEQELERISGLTQEEAINEQLQ
                     RVEEELSQDIAVLVKEKEKEAKEKVDKTAKELLATAVQRLAADHTSESTVSVVNLPND
                     EMKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARTALVNLVSDG
                     RIHPGRIEDMVEKARKEVDDIIREAGEQATFEVNAHNMHPDLVKIVGRLNYRTSYGQN
                     VLKHSIEVAHLASMLAAELGEDETLAKRAGLLHDVGKAIDHEVEGSHVEIGVELAKKY
                     GENETVINAIHSHHGDVEPTSIISILVAAADALSAARPGARKETLENYIRRLERLETL
                     SESYDGVEKAFAIQAGREIRVIVSPEEIDDLKSYRLARDIKNQIEDELQYPGHIKVTV
                     VRETRAVEYAK"
     gene            complement(2066..2275)
                     /locus_tag="POB55_002615"
     CDS             complement(2066..2275)
                     /locus_tag="POB55_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501525.1"
                     /translation="MNIDPEKFAELVVMSNPSKFEDAEDIAKESLKLYINAYRLAERY
                     STIATNCYDTAEVIKELKKTDLQLK"
BASE COUNT          851 a          322 c          426 g          676 t
ORIGIN      
        1 attattacat tatcaatagt tttataatcg agcttcaaaa ctttagaaaa tagtagaaat
       61 agcattcaat atagtgcaaa agtgcaaatt gataacttga cacttatctc ctataaaccg
      121 tacaattaat ttgtatgatt tatatataat ttcataaagt catattgaat ttcatataaa
      181 gagcaaaccc tagaaaagga ggtgtttgtg tgaatttatt aagcctccta ctcattttgc
      241 tggggatcat tctaggagtt gttggagggt atgttgttgc ccgaaatttg ttgcttcaaa
      301 agcaatcaca agctagacaa actgccgaag atattgtaaa tcaagcacat aaagaagctg
      361 acaatatcaa aaaagagaaa ttacttgagg caaaagaaga aaaccaaatc ctaagagaac
      421 aaactgaagc agaactacgt gaaagacgta gtgaacttca aagacaagaa acccgacttc
      481 ttcaaaaaga agaaaactta gagcgtaaat ctgatctatt agataaaaaa gatgagattt
      541 tagagcaaaa agaatcaaaa attgaagaaa aacaacaaca agtagatgca aaagagagta
      601 gtgttcaaac gttaataatg aagcatgaac aagaattaga acgcatctcc ggtctcactc
      661 aagaagaagc tattaatgag caacttcaaa gagttgagga agaactgtca caagatattg
      721 cagtacttgt taaagaaaaa gaaaaagaag ctaaagaaaa agttgataaa acagcaaaag
      781 aattattagc tacagcagta caaagattag cagcagatca cacaagtgaa tcaacggtat
      841 cagtagttaa cttacctaat gatgagatga aaggtcgaat cattggacga gaaggacgaa
      901 acatccgcac acttgaaact ttaactggca ttgatttaat tattgatgac acaccagaag
      961 cggttatatt atctggtttt gatccaataa gaagagaaat tgctagaaca gcacttgtta
     1021 acttagtatc tgatggacgt attcatccag gcagaattga agatatggtc gaaaaagcta
     1081 gaaaagaagt agacgatatt attagagaag caggtgaaca agctacattt gaagtgaacg
     1141 cacataatat gcatcctgac ttagtaaaaa ttgtagggcg tttaaactat cgtacgagtt
     1201 acggtcaaaa tgtacttaaa cattcaattg aagttgcgca tcttgctagt atgttagctg
     1261 ctgagctagg cgaagatgag acattagcga aacgagctgg acttttacat gatgttggta
     1321 aagcaattga tcatgaagta gaaggtagtc atgttgaaat cggtgtagaa ttagcgaaaa
     1381 aatatggtga aaatgaaaca gttattaatg caatccattc tcatcatggt gatgttgaac
     1441 ctacatctat tatatctatc cttgttgctg ctgcagatgc attgtctgcg gctcgtccag
     1501 gtgcaagaaa agaaacatta gagaattata ttcgtcgatt agaacgttta gaaacgttat
     1561 cagaaagtta tgatggtgta gaaaaagcat ttgcgattca ggcaggtaga gaaatccgag
     1621 tgattgtatc tcctgaagaa attgatgatt taaaatctta tcgattggct agagatatta
     1681 aaaatcagat tgaagatgaa ttacaatatc ctggtcatat caaggtgaca gttgttcgag
     1741 agactagagc agtagaatat gcgaaataat ttttgtctcc ctcacaaatt agtgagggag
     1801 cttttttaag ttgtagtctt aatctagtta gacagcactt tatcggtaat aactatatta
     1861 aacagtagtt atttgaaagt aagacggacc ttatattaaa taagaagtta ttgcttttaa
     1921 taaaaatgtt ttaggcttcg taattactat atttatatta tgtaaaccta taaagatgat
     1981 tgcttttcta tccaataaaa aagaagagaa gatgtaacac atcttctctt ctgcaatatt
     2041 aattaggatt tatttctaag ttgagttatt ttaattgtaa atctgttttc tttaattctt
     2101 ttataacttc tgcagtatca taacaatttg ttgcaattgt tgaatatctc tctgctaaac
     2161 gatatgcatt aatgtaaagc tttaaacttt ctttagctat atcctctgca tcttcgaatt
     2221 ttgatgggtt agacataacc actaattctg caaatttttc tggatcaata ttaat
//
LOCUS       DAMZKC010000252         2245 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.255, whole genome shotgun sequence.
ACCESSION   DAMZKC010000252 DAMZKC010000000
VERSION     DAMZKC010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.255"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            116..565
                     /locus_tag="POB55_002616"
     CDS             116..565
                     /locus_tag="POB55_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-inhibiting protein CHIPS"
                     /protein_id="HDI6501526.1"
                     /translation="MKKKLATTVLALSFLTAGISTHHHSAKAFTFEPFPTNEEIESNK
                     KMLEKEKAYKESFKNSGLPTTLGKLDERLRNYLKKGTKNSAQFEKMVILTENKGYYTV
                     YLNTPLAEDRKNVELLGKMYKTYFFKKGESKSSYVINGPGKTNEYAY"
     gene            complement(660..995)
                     /locus_tag="POB55_002617"
                     /pseudo
     CDS             complement(660..995)
                     /locus_tag="POB55_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500032.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
     gene            complement(1645..2136)
                     /gene="sak"
                     /locus_tag="POB55_002618"
     CDS             complement(1645..2136)
                     /gene="sak"
                     /locus_tag="POB55_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylokinase"
                     /protein_id="HDI6501527.1"
                     /translation="MLKRGLLFLTVLLLLFSFSSITNEVSASSSFDKGKYKKGDDASY
                     FEPTGPYLMVNVTGVDGKGNELLSPHYVEFPIKPGTTLTKEKIEYYVEWALDATAYKE
                     FRVVELDPSAKIEVTYYDKNKKKEETKSFPITEKGFVVPDLSEHIKNPGFNLITKVII
                     EKK"
BASE COUNT          795 a          374 c          326 g          750 t
ORIGIN      
        1 tttattaatt tcaagttaat taatatattt cgtcgattag attaaactca ttttaggaat
       61 ttcgcaaaac taactataaa tttaaatata gaatttaagg agaattaaca tcattatgaa
      121 aaagaaatta gcaacaacag ttttagcatt aagtttttta acggcaggaa tcagtacaca
      181 ccatcattca gcgaaagctt ttacttttga accgtttcct acaaatgaag aaatagaatc
      241 aaataagaaa atgttagaga aagaaaaagc ttataaagaa tcatttaaaa atagtggtct
      301 tcctacaacg ctaggaaaat tagatgaacg tttgagaaat tatttaaaga aaggcacaaa
      361 aaattctgct caatttgaaa aaatggttat tttaactgaa aataaaggtt actatacagt
      421 atatctgaat acaccacttg ctgaagatag aaaaaatgtt gagttactag gtaaaatgta
      481 taaaacatac ttctttaaaa aaggagagtc taaatcatct tatgtaatta atggtcctgg
      541 aaaaactaat gaatatgcat actaatagta gttacataaa ttaaaaggta gatatttctt
      601 ttttatataa aggtttggca gacatttcat aacttgccaa acctttatat atctaattat
      661 caaactgcac taaacttacc aaaactgctt attctattac ctgccttgtc tacctctcct
      721 gtcgctatat aacgacgttg tccactatta gcaatataag taatccatct atagccatta
      781 atgcaatatg cgccgtcata tttgatagtt gcgttattgg gtaatacacc tgtaattctt
      841 gaattagttg aatagccatc cctcacgtta ttacctttaa cattggcaac tgtgtaataa
      901 ccagtctctt ttttatacgg tacattgttt ttatcgagtg tataacctgc tggcactggt
      961 gaattctttt catttttagc tggtgtttta acattactga tacctgatac acacttccaa
     1021 taaaaataac cacaccattt aagatgcggt gtagcgactg taatatttct atgttgttga
     1081 gatatatgta tcgagtgaag ggcaaaaagg atatcaattg caggataagt gttaggttac
     1141 taggccactt aacaggctat atagttcact cctactatat acagttaatt ataacataaa
     1201 aagcaccccg taaactatta tacgggaatg ctaaagtcat atatactacg agggagtagt
     1261 atgaaaacta tgctctctat cataagaaaa aacacccagt gacatgcttg ggtgaacaag
     1321 gatagatgta aatagttgat gcatgtgtaa cacatcataa caaaaaacta gcccgaaagc
     1381 tagctataac atacaatcta aaaagacgtc ccttgaatac gtctagaaag attataacat
     1441 aaaaaaatag gcaagtaccg aagtacctgc ctaaaaaagg attatccact ttttcatcct
     1501 aactgatttc tccccataag tcacctaata tctgattagg tggggaagaa ccattcgtgc
     1561 atgaatgaga atttgatgaa agataatttt cactacacat attcaatcaa gacattgctt
     1621 tctataataa acaactattt tgttttattt cttttctata ataacctttg taattaagtt
     1681 gaatccaggg tttttaatat gctctgataa atctgggaca acaaaacctt tttctgttat
     1741 agggaaagac ttcgtttctt cttttttctt attcttatca taataagtga cttcgatctt
     1801 tgcgcttgga tctaattcaa ctactctaaa ctctttatat gctgtcgcat ctaatgccca
     1861 ttcgacatag tattcaattt tttcttttgt aagtgtagtc ccaggtttaa taggaaactc
     1921 gacataatga ggggatagca attcatttcc tttaccatca actccagtca catttaccat
     1981 caaatacggg cctgttggtt caaaataact cgcgtcatcg ccttttttat attttccttt
     2041 gtcgaatgaa cttgatgcac ttacctcatt agtaattgaa gaaaatgaga ataataacaa
     2101 taaaacagtt aaaaataata aacctctttt gagcatggcg cttcctccaa atataatata
     2161 tttgttttta tttatctctt tttatagcta acacccttat ttaagatgat aaatatcaac
     2221 aacttcattt tatattgaga aaata
//
LOCUS       DAMZKC010000253         2231 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.256, whole genome shotgun sequence.
ACCESSION   DAMZKC010000253 DAMZKC010000000
VERSION     DAMZKC010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.256"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..448
                     /locus_tag="POB55_002619"
     CDS             <1..448
                     /locus_tag="POB55_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDI6501528.1"
                     /translation="YSWIDDVKNYKFFNSKSDILFIIEGVLMYFDESVMTQLLDTIIK
                     KMGDHNLTFAIEFCSKTIANNTKRHQSVSKLSSQPVFKYGYNDLKKLNEILPNTIRVI
                     HEYNYFDYYKNRWGLFGYCRFIPYLKKRLNNKIVLMKYKVPKRQRH"
     gene            complement(820..2043)
                     /locus_tag="POB55_002620"
     CDS             complement(820..2043)
                     /locus_tag="POB55_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HDI6501529.1"
                     /translation="MTTFSEKEKIQLLADIVELQTENNNEIDVCNYLKDLFDKYDIKS
                     EILKVNEHRANIVAEIGNGSPILALSGHMDVVDAGNQDNWTYPPFQLTEKAGKLYGRG
                     TTDMKGGLMALVITLIELKEQNQLPQGTIRLLATAGEEKEQEGAKLLADKGYLDDVDG
                     LIIAEPTGSGIYYAHKGSMSCKVTATGKAVHSSVPFIGDNAIDTLLEFYNLFKEKYSE
                     LKQQDTKHELDVAPMFKSLIGKEISEEDANYASGLTAVCSIINGGKQFNSVPDEASLE
                     FNVRPVPEYDNDFIESFFQNIINDVDSNKLSLDIPSNHRPVTSDKNSKLITTIKDVAS
                     SYVEQDEIFVSALVGATDASSFLGDNKDNVDLAIFGPGNPLMAHQIDEYIEKDMYLKY
                     IDIFKEASIKYLKEK"
BASE COUNT          734 a          347 c          336 g          814 t
ORIGIN      
        1 ttacagttgg attgatgatg tcaaaaatta taaatttttt aatagtaagt cagatatatt
       61 gtttattatt gaaggtgtat tgatgtattt tgatgagagt gtaatgactc aattattgga
      121 cactattatc aaaaagatgg gagatcataa tttgacattt gcgattgaat tttgctcaaa
      181 aacaattgcg aataatacaa agagacatca atcggtatca aagttatcct cacaacctgt
      241 ttttaaatat ggttacaatg atttaaaaaa attgaatgaa attttaccaa atacaataag
      301 agtaatacat gaatataatt attttgacta ttataagaat cgatggggct tatttggata
      361 ttgtagattt ataccttatt taaaaaaaag gttaaacaat aaaatagtac ttatgaaata
      421 taaagttcca aagagacaga gacattaagt tcttagacaa tgtaaaaaag ctgatttcta
      481 taaattattt gatagaaatc agcttttttc aaatgtattt gataatttac agctcgttga
      541 gctgctgttt tctagggatt tatgtcccat tccttttacg agaaggtttt tacgagtttt
      601 atgtaactat tagaagctat ctttttatat gaccattagt caatatatgt atgacaaatc
      661 agcacaataa tttcagtttt acatcactta atcaatgaaa atgtgcttgt tgatatttga
      721 atatttacat tcaaacaata ttcatcgtta tttctatcaa cttaaaattc gcaataaaaa
      781 actgccagca tgcgaatagg gcagctgaca gcatcgttct tatttctctt ttaaatattt
      841 aatggaagcc tctttaaaaa tatcaatata tttcagatac atatcttttt caatatattc
      901 atcgatttga tgtgccatta atggattacc tggtccaaaa atggctaaat caacattgtc
      961 cttattatct cctaagaaac tagaggcatc tgttgcacct acaagcgctg aaacaaatat
     1021 ttcgtcttgt tctacataac tagaagctac atctttaatc gtagtaatta atttgctatt
     1081 tttatcgctt gttacaggtc ggtgattgct tggaatatcg agtgaaagct tattgctatc
     1141 cacatcatta atgatatttt ggaaaaacga ttctataaag tcgttatcat actcaggaac
     1201 tggtcttacg ttaaattcaa gtgaagcttc atctggtaca gagttaaatt gtttgccgcc
     1261 atttataatc gaacatacag ctgtaagacc agatgcataa tttgcatcct cttcagaaat
     1321 ttcttttcca atcaatgatt tgaacatagg cgcaacatct aattcatgtt tagtatcttg
     1381 ttgtttaagc tctgaatatt tttctttaaa tagattataa aattcaagca gtgtatcaat
     1441 tgcattgtca ccaataaatg gaactgagct atggacagct ttaccagttg cagttacttt
     1501 acatgacata gaccccttat gtgcataata aattccagat ccagttggtt cagcaataat
     1561 taagccatcg acatcgtcta aatagccttt atcagctaat aatttggcac cttcttgttc
     1621 tttctcttcg ccagcagtag ctagtaatct tatcgttcct tgaggcaatt gattttgttc
     1681 ttttaattca atgagtgtga tgaccaaagc catcagaccg cctttcatat ccgttgtgcc
     1741 tcgaccgtat aatttgccag ctttttctgt gagttgaaaa gggggataag tccaattatc
     1801 ttgatttcct gcatcaacaa catccatatg accactcaat gcgagtatag gtgagccgtt
     1861 accgatttct gcaacgatat tggcgcggtg ttcattaact ttcaaaattt cagatttaat
     1921 atcgtacttg tcgaataaat cttttaaata attacaaacg tctatttcat tattattttc
     1981 agtttgtagt tcaacaatat ctgctagtaa ttgaattttt tctttttcac taaaagttgt
     2041 cattaagctc acaccttttc aaagtagtat atatatttat ataaacataa tttttattct
     2101 taaacataat ttaaaatgca gaaagtagcg tgttatttga tttcgttcta tgaaattaac
     2161 tgtctagtat caacgatctt attaacactg atatcgggta tgcttaattt tatagtaaat
     2221 tgtatgagaa a
//
LOCUS       DAMZKC010000254         2214 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.258, whole genome shotgun sequence.
ACCESSION   DAMZKC010000254 DAMZKC010000000
VERSION     DAMZKC010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.258"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..451
                     /locus_tag="POB55_002621"
     CDS             <1..451
                     /locus_tag="POB55_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HDI6501530.1"
                     /translation="MKLPLISGYFQLFKTYFVTNELVLFYKNGITLQSIVDVYINHSS
                     DPFRQFLGKYLLTYSEMGYGLPQILEKLKCFKPQLIKFVLQGEKRGKLEVELKLYSQI
                     LVKQIEDKAIKQTQFLQPILFLILGLFIVAIYLVIMLPMFQMMQSIK"
     gene            465..776
                     /locus_tag="POB55_002622"
     CDS             465..776
                     /locus_tag="POB55_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDI6501531.1"
                     /translation="MFKFLKKTQAFTLIEMLLVLLIISLLLILIIPNIAKQTAHIQST
                     GCNAQVKMVNSQIEAYALKHNRNPSSIEDLIADGFIKEAQKTCKSGETITISNGEAVA
                     N"
     gene            754..1200
                     /locus_tag="POB55_002623"
     CDS             754..1200
                     /locus_tag="POB55_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HDI6501532.1"
                     /translation="MEKQLQIRKQSAFTMIEMLVVMMLISIFLLLTMTSKGLSNLRVI
                     DDEANIISFITELNYIKSQAIANQGYINVRFYENSDTIKVIENNKIRFLKLKVGKIIN
                     VAKVDIIAFDKKGNINKFGSITIDNNNSIYRIIFHIEKGRIRYEKL"
     gene            1187..1486
                     /locus_tag="POB55_002624"
     CDS             1187..1486
                     /locus_tag="POB55_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGE"
                     /protein_id="HDI6501533.1"
                     /translation="MKSYKCKGSFLIDSMAGFLLIGLITLLLIPMMNQMQASINHKLQ
                     TIDASKVILTTVSKINKEELKKGVTIGKYDIKQSDQQICAISKNTTSYQKTCIQY"
     gene            1404..1901
                     /locus_tag="POB55_002625"
     CDS             1404..1901
                     /locus_tag="POB55_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDI6501534.1"
                     /translation="MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVI
                     SITLLIVPDLIRLSKTFLIESRDLTTVDFEFFSRDILDDFKGVDRNDIEIRQHRIILH
                     KGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNVQT
                     KTIYV"
     gene            1998..2144
                     /locus_tag="POB55_002626"
     CDS             1998..2144
                     /locus_tag="POB55_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501535.1"
                     /translation="MMLTAIYFSIFTFYISQYSLKLKTLYNIETDYNYKIVLLLKEGK
                     THES"
     gene            2134..>2214
                     /locus_tag="POB55_002627"
     CDS             2134..>2214
                     /locus_tag="POB55_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HDI6501536.1"
                     /translation="MNHDKSPIILIGFMGTGKSTIGKYVAD"
BASE COUNT          877 a          251 c          345 g          741 t
ORIGIN      
        1 gatgaaacta ccgctaatat caggctattt ccaattattt aaaacttatt ttgtaactaa
       61 tgaattagtg ttgttttata aaaatggtat tacacttcaa tcaatagtag acgtttatat
      121 taaccatagt agtgatccat ttagacagtt tctaggtaaa tacttattaa cttattcaga
      181 aatgggatat ggtttgcctc aaattttaga aaaactaaaa tgctttaagc ctcaattaat
      241 taagtttgtg ctacaaggtg aaaagagagg gaagctagaa gtagaactaa agttatattc
      301 acaaatatta gtaaaacaaa tagaagataa agcgataaaa cagactcagt ttttacagcc
      361 tattttattt ttgattttag gtttatttat tgtcgcaatt tatttagtaa ttatgttacc
      421 aatgtttcaa atgatgcaaa gtataaaata atggaggtta atatatgttt aaatttctta
      481 agaaaactca agcgtttaca ttgatagaga tgctattagt gttattaatc atcagtttat
      541 tattaatttt aatcattcca aatattgcta aacaaactgc tcacatacaa tcaacaggtt
      601 gtaatgcaca ggtaaaaatg gttaatagtc aaattgaagc gtatgcattg aaacataata
      661 gaaatccatc gtctattgaa gacttaattg cagatggttt tataaaagaa gcacaaaaga
      721 catgtaaatc aggagagaca atcacaatta gtaatggaga agcagttgca aattagaaag
      781 cagtcagcat ttactatgat tgagatgctt gtggtaatga tgttaatcag tatatttcta
      841 cttttgacaa tgacatctaa aggattaagc aatcttagag taatagatga tgaggcaaat
      901 atcatttctt ttattactga attgaattat attaagtcgc aagctatagc aaatcaagga
      961 tatatcaatg ttagatttta tgaaaacagt gacactatta aagtaataga gaataataaa
     1021 atacgatttc taaaattaaa agtaggcaaa ataattaatg ttgcaaaagt tgatattatt
     1081 gcctttgata aaaaagggaa tatcaataaa tttggtagca taacaattga caataacaat
     1141 tcaatttata gaataatatt ccatattgaa aaaggaagaa ttcgttatga aaagctataa
     1201 gtgtaaaggt tcattcttaa tagatagtat ggctggattt ttgctaattg gattgattac
     1261 attactattg ataccaatga tgaatcaaat gcaagcgagt ataaaccata aactacaaac
     1321 aattgatgct tctaaagtaa ttttgacgac tgtatctaaa attaataaag aagaacttaa
     1381 gaagggggta actataggga agtatgatat taagcaaagt gaccaacaaa tttgtgctat
     1441 ttcaaaaaat accacttctt atcaaaagac atgtatacag tattaatgtc aaagcttttt
     1501 cgctcattga aatgttagta gcgatgatgg ttataagtat aactttacta attgttccag
     1561 acttaattag acttagtaaa acttttctaa ttgaaagtag ggatttaaca actgtagatt
     1621 tcgaattttt ctcaagagat attctagatg attttaaagg agtagataga aacgatattg
     1681 aaattaggca acaccgtatc attttacata aaggtgaaga aatgatcgaa tacaaattaa
     1741 ttaataataa aattattaaa gttgtaaatg acagaggaaa tataacaatg attaataatg
     1801 ttacagcatt tactgcaaat atctactata aatccattat taaaataacg ataacagtta
     1861 aagtcggtac aaatgtgcag actaaaacta tttatgtata acttactagt atttaaatga
     1921 gtaccattaa aaaggatgtg aaataattta ttaattttga tttatataaa gaaaaaggat
     1981 atgttacgcc gttcttattg atgctaacag caatttattt ttccattttc actttttata
     2041 tcagtcaata tagtttaaaa ttaaagacat tatataatat tgaaactgac tataactata
     2101 agattgtttt gttattgaaa gaagggaaaa cgcatgaatc atgataaatc accaataatt
     2161 ttaattggtt tcatgggtac cggtaaatct acgattggta aatacgttgc agat
//
LOCUS       DAMZKC010000255         2214 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.259, whole genome shotgun sequence.
ACCESSION   DAMZKC010000255 DAMZKC010000000
VERSION     DAMZKC010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.259"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            753..1622
                     /locus_tag="POB55_002628"
     CDS             753..1622
                     /locus_tag="POB55_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HDI6501537.1"
                     /translation="MEMSRLNLTDDNDIDEIIAWRGEQEELEQKRDTYKKRYHEFEME
                     IARLESLTKDKELLDSDKLKDEYELKKGKMNTLIDEYSAVHYQCQNNINKTQSIVSHI
                     NYLNQELKDQQEIFQLAEIVGGKNNKNLTLENFVLIYYLDQIIAQANLRLATMSDNRY
                     QLIRREAVSHGLSGLEIDVFDLHSNKSRHISSLSGGETFQSSLALALGLSEIVQQQSG
                     GISLESIFIDEGFGTLDQETLETALDTLLNLKSTGRMVGIISHVSELKNRIPLVLEVK
                     SDQYQSSTRFKRN"
     gene            complement(1693..2055)
                     /gene="mscL"
                     /locus_tag="POB55_002629"
     CDS             complement(1693..2055)
                     /gene="mscL"
                     /locus_tag="POB55_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HDI6501538.1"
                     /translation="MLKEFKEFALKGNVLDLAIAVVMGAAFNKIISSLVENIIMPLIG
                     KIFGSVDFAKEWSFWGIKYGLFIQSVIDFIIIAFALFIFVKIANTLMKKEEAEEEAVV
                     EENVVLLTEIRDLLREKK"
BASE COUNT          888 a          292 c          352 g          682 t
ORIGIN      
        1 tagagaatca aattaatgaa gaaacatcta tattgaagaa tctcgaaata aaattagatc
       61 gctacgataa aacaaaattg gacttaaatg ataaagaaag ctttataagt gaaattaaat
      121 ctgctgtaaa tattggagat caatgtccga tatgtggtaa tgaaattcag gatttagggc
      181 atcatattga ttttgacagt attgctaaac gtcaaaatga aattaaagaa attgaagcaa
      241 atatccacgc tataaaatcg aatattgctg tgcataattc tgaaattaaa tttgttaatg
      301 aaaaaatatc gaatattaat attaaaacgc aaagtgattt ttcacttgaa gtattgaata
      361 agcgtctgct agaaaatgaa aatgcattga ataatcaaag agatcttaat aaatttatag
      421 aacaaatgaa agaagaaaaa gataatctaa cgttgcaaat tcataataaa caattgcgtc
      481 taaataaaaa tgaatctgag ttgaaattat gtcgagatct catcactgaa tttgaaacac
      541 tctcgaaata taataatatc actaattttg aggtggatta taagaagtat gttcaagatg
      601 tgaatcagca tcaagaactc tcaaaggaga ttgaagataa gttaatgcaa ttgtctcaaa
      661 gaaagttaat tgagcaaaat aatctaaatc attatgaaaa tcaactagaa acttacaata
      721 atgacttaga attgaatgaa cagtctattg aaatggaaat gtcgaggctg aatttaactg
      781 atgacaatga tatagatgaa ataatagcct ggaggggcga gcaagaggaa ttagagcaga
      841 aaagggatac ttataaaaaa cgttatcatg aatttgaaat ggaaatagct aggttagaat
      901 cattaactaa ggataaagag ttattggact ctgataaatt aaaagatgaa tatgagctaa
      961 aaaaaggaaa gatgaataca ctgatagatg aatactctgc tgttcattat caatgtcaaa
     1021 ataatattaa taaaacacaa tctatagttt cgcatattaa ttacttaaat caagaattaa
     1081 aggatcaaca agaaatattt caattggctg aaattgtcgg tggtaagaat aacaaaaatc
     1141 ttacattgga aaactttgtc ttaatttact atttagatca aattattgct caagcaaatc
     1201 tgagattagc aacaatgtca gataatcgat accaactaat taggcgagaa gcggtttctc
     1261 atggtcttag tggcctagaa attgatgtat ttgatttgca ttcaaataag tctagacata
     1321 ttagctcgtt atcaggtgga gaaactttcc aatcgtcgct tgcattagct ttagggttaa
     1381 gcgaaattgt acagcagcaa tcaggaggta tttcactaga atcaatattt attgatgaag
     1441 gattcggtac attagatcaa gaaacgcttg aaacagcgtt agacacttta ttaaatctta
     1501 aatcaactgg tagaatggtt gggattattt cacatgtgag cgaattgaaa aatagaatac
     1561 ctttagtttt agaagtgaaa tcagatcaat atcagagttc aacaagattc aaaagaaatt
     1621 aaaacataaa aaatagagat ggtactttgg aatgtataga acaaccttag cccatctctt
     1681 tttatttaaa aattattttt tctcacgtaa taaatctctg atttcagtta ataacacaac
     1741 attttcttcc acaactgctt cttcttcggc ttcttctttc ttcattaatg tatttgcaat
     1801 cttaacaaag atgaataaag caaacgcgat gataataaag tcgataacag attggataaa
     1861 taaaccgtat ttaatacccc agaatgacca ttctttagca aaatcaactg atccgaaaat
     1921 tttaccaatt aatggcataa tgatattttc tactaatgaa gatataatct tgttgaaagc
     1981 tgcacccatc acaacagcaa ttgctaaatc taagacgtta ccttttaagg cgaactcttt
     2041 gaattctttt aacattacac tcaacctctc ttttttaaat gtaacctcaa ttatacaaca
     2101 aataaaaaaa ttagaaaata gattattcaa gaaaatttga ttatcgcaaa gacctttgtt
     2161 atcctataaa ggtgcgtgag ttattgaata aaatgtttag ataatatttt tatt
//
LOCUS       DAMZKC010000256         2208 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.260, whole genome shotgun sequence.
ACCESSION   DAMZKC010000256 DAMZKC010000000
VERSION     DAMZKC010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.260"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            332..1000
                     /gene="queC"
                     /locus_tag="POB55_002630"
     CDS             332..1000
                     /gene="queC"
                     /locus_tag="POB55_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HDI6501539.1"
                     /translation="MESVLNNEKAIVVFSGGQDSTTCLFYAKKHFKEVELVTFNYGQR
                     HDTEIEVAKQIAQDQGMKHHVLDMSLLSQLTPNALTQHDMEITNNEDGIPNTFVPARN
                     LLFLSFAGALAYQIGAKHIITGVCETDFSGYPDCRDSFIKSMNVTLSLAMDKDFVIHT
                     PLMWLNKAETWKLSDELEVLDYIRTKTLTCYNGIIGDGCGECPACHLRQRGLNQYLES
                     KGAL"
     gene            1002..1421
                     /gene="queD"
                     /locus_tag="POB55_002631"
     CDS             1002..1421
                     /gene="queD"
                     /locus_tag="POB55_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HDI6501540.1"
                     /translation="MLQQIYPSTTHPYQFELNKDFNFSAAHHIPCEEAGICQNVHGHT
                     YFVNLTIVGDKLDDTGFLVNFSHLKKMIHGKFDHQLLNNLPAFKNKIPSTEIVAETIY
                     QIVKENLASLEHQPKCIQVFVRETPTSYVVFRPKEQV"
     gene            1425..2138
                     /gene="queE"
                     /locus_tag="POB55_002632"
     CDS             1425..2138
                     /gene="queE"
                     /locus_tag="POB55_002632"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HDI6501541.1"
                     /translation="MAKIPVLEIFGPTIQGEGRVIGRKTMFVRTAGCDYRCSWCDSAF
                     TWDGSAKGDIKLMTAEEIYDELKRIGGDLFNHVTISGGNPALIKGIQELVDLFQDKGI
                     FSALETQGSKFQPWMTQIDDLTISPKPPSSTMTPDLKKLDEVITQCVPSSLNLKVVVF
                     DDKDYDFAKMIHHRYPDIPFYLQVGNPYLSDSVDNHTEKLLERYEQLVDLVMQSNDMN
                     HVYVLPQLHTLLWSNKKGV"
BASE COUNT          779 a          353 c          374 g          702 t
ORIGIN      
        1 gtgttttatt tttataatat gtattggatg tagctaaaac tttaaaagca ttgtcattat
       61 cctgaccttg attttaacta atatatagta tattctattc atcgtacata aatgaatatc
      121 agaggttcct agctgaaacc ctctataaaa aactagacat tgaaatttca aacattcgtt
      181 ggtaaacctt taatatatat tataagagca acccatttct ttcgtctatc ttttatggag
      241 ataggctttt tttattttat aaattaaaaa cgggcttatc aatgccatta acctctttat
      301 taatattatt aatgatagga gcaattacaa aatggaaagt gtattaaata atgaaaaagc
      361 catcgttgtc tttagtggtg gtcaagacag tacaacatgt ctcttttatg caaaaaaaca
      421 tttcaaagaa gttgaactcg taacgtttaa ttatggccaa agacatgata ctgaaattga
      481 agtcgcaaaa caaattgcac aagatcaagg aatgaaacat cacgttttag atatgtcatt
      541 attatcacaa cttactccaa acgcattaac acaacatgat atggaaatta ctaataatga
      601 agatggtata cctaatacat ttgttccagc tagaaattta cttttcttgt cgtttgcagg
      661 cgctctagct tatcaaattg gggctaagca tattattaca ggcgtatgtg aaacagactt
      721 ttcaggctac ccagactgtc gcgatagttt tataaaatca atgaacgtaa cattaagcct
      781 agctatggac aaagattttg tcattcatac tcctttaatg tggttaaaca aagcagaaac
      841 gtggaaatta agtgatgaac tcgaagtttt agattatatt cgtacaaaaa cattaacatg
      901 ctataacggt atcattgggg atggctgtgg tgaatgtcca gcttgtcatt tacgtcaacg
      961 tggactaaat caatatcttg aaagtaaagg agcgctttaa aatgttacaa caaatctatc
     1021 ctagtacaac gcatccatat caattcgaat taaataaaga ctttaatttt tcggctgcac
     1081 atcacattcc ttgtgaagaa gcaggtattt gtcaaaatgt ccatggtcat acttactttg
     1141 ttaatttaac aattgtcggt gataaactag atgacactgg cttcttagtg aactttagcc
     1201 atttgaaaaa gatgatacac ggtaaatttg accatcaact gttaaataac ttacctgctt
     1261 ttaaaaacaa aatcccttca actgaaatcg tagcggaaac aatttatcaa attgttaaag
     1321 aaaatttggc atcgctcgaa caccaaccaa aatgtattca agtatttgta agagaaacac
     1381 caacaagtta tgttgtattt agaccaaagg aacaggtgta actaatggct aaaattcctg
     1441 tactcgaaat attcggtcca accattcagg gtgaaggtcg agtaatcggt agaaaaacga
     1501 tgtttgtgag aacagctgga tgtgattatc gctgtagctg gtgtgattct gcatttactt
     1561 gggatggtag tgctaaaggc gatataaaac tcatgaccgc tgaagaaatt tatgatgaat
     1621 taaaacgaat tggtggcgat ttatttaatc acgttacaat ttctggtggt aacccagcat
     1681 taattaaagg tatccaagag ttagttgact tatttcaaga taaaggtatt ttcagcgcac
     1741 tggagacaca aggcagtaaa ttccaacctt ggatgacaca aattgatgat ttaacgatta
     1801 gtccaaaacc accaagttca actatgacac ctgatttaaa aaagttagat gaagtgatta
     1861 cacaatgcgt accttcctca ttaaacttaa aagtagttgt attcgacgat aaagattatg
     1921 attttgccaa aatgatacac caccgttatc cagatattcc cttctattta caagttggta
     1981 acccatattt atcagacagc gtagataatc ataccgaaaa gttgttagaa cgctacgagc
     2041 agttggtgga tttagttatg caaagtaatg atatgaatca cgtttatgtg ttaccacaac
     2101 ttcatacatt actttggagt aataaaaaag gtgtataaag gataacttat tttacttttt
     2161 acaagtcata tctaatgaat aacaatatat taattgatat aatagttg
//
LOCUS       DAMZKC010000257         2207 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.261, whole genome shotgun sequence.
ACCESSION   DAMZKC010000257 DAMZKC010000000
VERSION     DAMZKC010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.261"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            63..1052
                     /gene="trpS"
                     /locus_tag="POB55_002633"
     CDS             63..1052
                     /gene="trpS"
                     /locus_tag="POB55_002633"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HDI6501542.1"
                     /translation="METLFSGIQPSGIPTIGNYIGALKQFVDVQNDYDCYFCIVDQHA
                     ITMPQDRLKLRKQTRQLAAIYLASGIDPDKATLFIQSEVPAHVQAGWMLTTIASVGEL
                     ERMTQYKDKAQKAVEGIPAGLLTYPPLMAADIVLYNTNIVPVGDDQKQHIELTRNLVD
                     RFNSRYNDVLVKPEIRMPKVGGRVMSLQDPTRKMSKSDDNAKNFISLLDEPNVAAKKI
                     KSAVTDSDGIIKFDRDNKPGITNLISIYAGLTDMPIKDIEAKYEGEGYGKFKGDLAEI
                     VKAFLVEFQEKYESFYNSDKLDDILDQGRDKAHKVSFKTVKKMEKAMGLGRKR"
     gene            complement(1094..1975)
                     /locus_tag="POB55_002634"
     CDS             complement(1094..1975)
                     /locus_tag="POB55_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6501543.1"
                     /translation="MQNKSKSPFKIALSRFIHNKIAMLSIIFLLIITIISIIAPLIAP
                     FPVNQQDLLNIKGEMTAQNILGTDSGGRDNFSRLLYAGRISLSIGITSTIGMLLIGIT
                     VGVISGYFGGIVDTLLMRITEFVMLFPFLIFAIVLNAALGDKIKNPYGSAIILVLVII
                     VLSWGGIARLVRGKVLQEKENEYFLAAKSIGTPTYKIILKHLLPNILSVVIVQATLLF
                     AGMIVVESGLSFLGFGISKAIPSWGNMLSDAQEGDVISGKPWIWMPPAIMITLTILSI
                     NFVGEGLKDAFNPRGRR"
     gene            complement(1987..>2207)
                     /locus_tag="POB55_002635"
     CDS             complement(1987..>2207)
                     /locus_tag="POB55_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDI6501544.1"
                     /translation="LGADIVSILGGAVITETIFSYNGIGKLFLESVIGQDYPLMMALT
                     LFFSFLGLLGNLISDITYGFIDPRIRSN"
BASE COUNT          815 a          377 c          338 g          677 t
ORIGIN      
        1 tttttaagaa atactgttat aatgataatt attaaaatat tactaagaaa gtaggcattt
       61 aaatggagac attattttca ggcatccaac ctagtggaat tcctactatt ggaaattata
      121 ttggcgcact aaaacaattt gttgatgtgc aaaatgacta tgattgttat ttctgtatcg
      181 tagatcaaca tgcaattaca atgccacaag atcgtttaaa attacgtaaa cagaccagac
      241 aattagcagc gatttattta gcttctggta tagatccaga caaagcaaca ttgttcatac
      301 aatctgaagt ccctgcacac gtacaagcag gatggatgtt aactacgatt gcttctgttg
      361 gagaattaga gcgtatgacg caatacaaag ataaagctca gaaagcagtt gaaggtatac
      421 ctgctggtct attaacatat ccacctttaa tggcagctga tattgttctt tacaatacta
      481 atatcgttcc agttggagat gaccaaaagc agcatatcga attgactcgt aaccttgtag
      541 atagatttaa tagtcgctat aatgatgtgc ttgtgaaacc tgaaattcgt atgcctaaag
      601 ttggtggacg tgtcatgagt ttacaagacc caacaagaaa aatgagtaag agtgatgata
      661 atgctaaaaa cttcatttca ttattagacg agccgaatgt tgcagctaaa aaaattaaaa
      721 gcgcagtaac tgattcagat ggtattatta aatttgatcg tgacaacaag ccaggtataa
      781 caaatttaat ttcaatatac gctggattaa cagacatgcc aattaaagat attgaggcaa
      841 aatatgaggg cgaaggttat ggtaaattta aaggtgacct tgctgaaata gttaaagcat
      901 ttttagtaga attccaagaa aaatacgaaa gtttctataa ctcagataaa cttgatgata
      961 ttttagatca aggtagagat aaagcacaca aagtttcatt taaaactgtc aaaaaaatgg
     1021 aaaaagcgat gggtttagga cgtaagagat aaaataaaga ggtactagaa ttaactagtg
     1081 cctcttttat tatttaacgt ctacctctag gattaaaagc atctttaagc ccttccccta
     1141 caaagtttat acttaatata gttaatgtaa tcattatagc aggtggcatc catatccacg
     1201 gtttaccact tataacatcc ccttcttgag catcactcaa catattaccc caagatggta
     1261 ttgctttact aattccgaat cctaaaaagc tcaatcctga ttccactaca atcataccgg
     1321 caaataacaa tgttgcttgt acgataacta cacttaatat attcggcaaa agatgtttca
     1381 aaataatttt atatgtgggt gtaccaattg attttgctgc caaaaagtat tcattttctt
     1441 tttcttgaag tactttacca cgaacaagtc ttgcaatacc tccccaactt aatacgataa
     1501 taactagaac aagaattatg gcagatccat aaggattttt aattttatct ccaagtgcag
     1561 catttaatac aattgcaaat attaaaaatg gaaataacat aacaaattcg gttattctca
     1621 ttaataatgt atcaacaata cctccaaaat aaccagaaat cactccaact gtaattccaa
     1681 tcaaaagcat tcctattgta gatgtaattc caatggataa tgaaatacga cctgcatata
     1741 acaaacgact aaagttatcc ctaccaccag agtctgtacc aagaatgttt tgtgctgtca
     1801 tttcaccttt tatatttaat aaatcttgtt ggttcactgg aaaaggagct attaatggcg
     1861 ctataattga tataatagtt atgattaata aaaaaataat cgataacatt gcaattttat
     1921 tatgaataaa tctagacaat gcaattttaa aaggcgattt tgacttattt tgcattccaa
     1981 tcccccctag ttacttctaa ttcttggatc tataaatcca taagtaatat cagaaatcaa
     2041 attacccagt aaacctaaaa atgagaaaaa caacgttaat gccatcatta atggatagtc
     2101 ttgacctatt accgattcta aaaataattt accgataccg ttatatgaaa agatagtctc
     2161 agtaatcaca gctccaccta aaatacttac aatatcagca cctaaaa
//
LOCUS       DAMZKC010000258         2200 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.262, whole genome shotgun sequence.
ACCESSION   DAMZKC010000258 DAMZKC010000000
VERSION     DAMZKC010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.262"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(154..798)
                     /locus_tag="POB55_002636"
     CDS             complement(154..798)
                     /locus_tag="POB55_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HDI6501545.1"
                     /translation="MSQNTNHSYYHQNQHAQSISKVWLYFMYYWIIFGIGCYLGQFLP
                     LSWRQPLSFGLLIIILATLVFERARRFGLIISHIYAVVIGLLSYATFTTYLQNLGPDI
                     FYKNIALAIFAFIAFGIIGYFFVGDASSIGKYLFVTLITLIIASLIGIFLQNPIFYTI
                     ITVVSLLLFLLYTLYDFNRLKRGDYSPREMGFNLFINLLNIIKDILYLANMFRR"
     gene            1159..1956
                     /locus_tag="POB55_002637"
     CDS             1159..1956
                     /locus_tag="POB55_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDI6501546.1"
                     /translation="MKKLAFAITATSGAAAFLTHHDAQASTQHTVQSGESLWSIAQKY
                     NTSVESIKQNNQLDNNLVFPGQVISVGGSDAQNSSNTSPQAGSASSHTVQAGESLNII
                     ASRYGVSVDQLMAANNLRGYLIMPNQTLQIPNGGSGGTTPTATTGSNGNASSFNHQNL
                     YTAGQCTWYVFDRRAQAGSPISTYWSDAKYWAGNAANDGYQVNNTPSVGSIMQSTPGP
                     YGHVAYVERVNGDGSILISEMNYTYGPYNMNYRTIPASEVSSYAFIH"
BASE COUNT          821 a          360 c          300 g          719 t
ORIGIN      
        1 atatctttac aattaaggta atatattttt attgctttat cagtaaaatc tcagtctaag
       61 tttctaactt atgacaatcg ctccagttta aggttgatat tcattattga ttattccaaa
      121 actttataca aatttcttta ttaatttaac tgtttatctt ctgaacatat tagcaagata
      181 aagtatatcc ttaataatat tcaacaaatt aataaataga ttaaatccca tttctcttgg
      241 tgaatagtca cctcttttta aacgattaaa atcatacaaa gtataaagta gaaataacaa
      301 caaactaacg acggtaataa tagtgtaaaa aataggattt tgaagaaaaa taccaattag
      361 actcgcaata attaatgtta ttaatgtaac gaataaatat ttgcctatac tcgatgcatc
      421 tccaacgaag aaataaccaa taataccaaa tgctataaat gcaaaaattg ctaatgcgat
      481 atttttatag aaaatatctg gtcctaaatt ttgtaaatac gtggtaaacg ttgcgtatga
      541 caataagccg atcactacag cgtaaatatg tgaaataatt aaaccgaacc gtctcgctct
      601 ttcaaaaaca agtgttgcta aaataataat cagtaatcca aatgacaagg gttgtcgcca
      661 acttaatggt aaaaactgac ctagatagca tcctatgcca aatataatcc aataatacat
      721 aaaataaagc cacactttac ttattgattg agcatgctgg ttttgatgat aatatgaatg
      781 atttgtattt tgcgacaaaa taattcctcc ttctaccgtc gttttatgcg tcaaatttta
      841 cagtaaattt tgcttcatca aaagaaataa cctttaataa gtatagtaat tttacatttt
      901 acaatgttac aaaatataat tttttataat tagttaaaat cactaaaacg cttttataca
      961 ctatcaaatc agcatttata aaaatatgaa ccgctatcct aaaatgttaa taatattaca
     1021 agataataac aaaccacaca aagctactta tttttgataa tatggaaatc gtaatataaa
     1081 acgaaaactt aatttactat ataaattgtc ttaataattt ttaaaagtag taaaacttaa
     1141 ttttaaggag gagtcccttt gaaaaaatta gcatttgcaa taacagcaac atctggtgca
     1201 gctgcatttt taacgcatca tgatgcacaa gcttctacac aacatacagt acaatctggt
     1261 gaatcattat ggagtattgc tcaaaaatac aacacttcag tagagagtat taaacaaaat
     1321 aaccaattag ataacaactt ggtattccct ggtcaagtta tctcagtagg tggaagtgat
     1381 gcacaaaatt cgtcaaacac ttctccacaa gctggttcag catcatctca tactgtacaa
     1441 gctggtgaat cattaaatat cattgctagc agatatggtg tttcagttga tcaattaatg
     1501 gcagccaata acttacgtgg ttatttaatt atgcctaacc aaacattaca aattcctaat
     1561 ggtggatcag gtggtacaac accaacagct acaacaggta gcaatggcaa tgcatcatct
     1621 tttaatcacc aaaatttata cactgctggt caatgtacat ggtacgtatt tgaccgtcgt
     1681 gctcaagctg gtagtccaat tagcacatat tggtcagacg ctaagtattg ggctggtaac
     1741 gcagctaatg atggttacca agtaaacaac acaccatcag ttggttcaat tatgcaaagc
     1801 acacctggcc catatggtca tgttgcttat gttgaacgtg tcaatggtga tggtagtatc
     1861 ttgatttctg aaatgaatta cacatatggt ccatacaata tgaactaccg tacaattcca
     1921 gcttcagaag tttctagcta tgcattcatc cattaattaa ataaattgta ctgatatata
     1981 ctagcaattc acatcatgtg agattgctag ttttttattt ttgaaaaaaa ttttcatttt
     2041 ggtacaaaaa attatctcac ccttccctat catacatatt tatattttgt atgaatggta
     2101 gttaggtaaa aattaacaac ctacctattt gattaaatta aatgcataat atattaaaaa
     2161 tcaaaataat gcaactaagt tgactatata atgatttaat
//
LOCUS       DAMZKC010000259         2199 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.263, whole genome shotgun sequence.
ACCESSION   DAMZKC010000259 DAMZKC010000000
VERSION     DAMZKC010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.263"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..559
                     /locus_tag="POB55_002638"
     CDS             <1..559
                     /locus_tag="POB55_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4888 domain-containing protein"
                     /protein_id="HDI6501547.1"
                     /translation="KFLGKTLVASALVLTTLGTGLHSSYLGLDTNKVVKTAKAEEKMT
                     DGQLWKKVRDSLHDSDIILSNEYETINVTYLLSNGYSSSVSAPGNDDGGHLTQSIDFK
                     GLKQIDLTKENVYDDFNKKLDAKNTWNSLTEKLKGLGLLQNGQKVSIYSSDSSSPVSG
                     KVGEGVTSGGENTLTKRFINKITID"
     gene            complement(1033..1731)
                     /locus_tag="POB55_002639"
     CDS             complement(1033..1731)
                     /locus_tag="POB55_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BsaG protein"
                     /protein_id="HDI6501548.1"
                     /translation="MIINELKSCKLKFSKQALTFVPIIVTILFILFINWYLNVNLWNG
                     RQISLFTASFNAITSLLISINVYQVINFEENIGHFNHILGKANRLNWLNASMIFTYTI
                     TAICILLASINLLWHSHDMKITLMFIGVSLFFNVIILLLLFIFSIFIKDVMAIVVGVL
                     MFIFNVYFGLEVLGDHSWFYLPITYATRYVYMFIEGSIPVTLTLAIYIIITLLLAVLL
                     FKGFNKWSGRTIKD"
     gene            complement(1728..>2199)
                     /locus_tag="POB55_002640"
     CDS             complement(1728..>2199)
                     /locus_tag="POB55_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HDI6501549.1"
                     /translation="EIRAKIFILSIAFLVHCFLVFIIAYIGDLIINPHPVNAMLLLVT
                     ILLMYVVSLPLIPLNFLLTRYFGVFVSILINLVLSVICVLFLTLKSLFWVLPWGIMQR
                     IPLITLGILPNGLVVNHNSKYFNDLNALYISIIVSIIIFAIVTFLNNKKSWRLK"
BASE COUNT          857 a          364 c          288 g          690 t
ORIGIN      
        1 aaaattttta ggtaaaacat tagtagcaag tgctttagta ttaacaacat taggaacagg
       61 cttacattct tcatacttag gattagatac aaataaagtt gttaaaacgg ctaaagcaga
      121 agaaaaaatg acagatggtc aattatggaa aaaagttaga gattcattac atgattctga
      181 tattatttta agtaacgagt acgaaacaat caacgttact tatttattaa gcaatggata
      241 ttctagcagc gtttctgctc ctggaaatga tgatggaggc catctaacac aatctataga
      301 ctttaaagga ttaaaacaaa ttgatttaac taaggaaaat gtctacgatg actttaacaa
      361 aaagctagat gctaaaaata catggaattc tctaaccgag aagttaaaag gtcttggttt
      421 actacaaaat ggccaaaaag tatctattta ttcttcagat agctcttctc cagtctcggg
      481 taaagtggga gaaggagtaa ctagtggtgg cgaaaatact ctaactaaac gcttcatcaa
      541 caaaatcaca attgattaaa ccttaaactc aaatttcctt aattcataaa acattaataa
      601 ctaacccttt cttacaaacc acctagccca taatatatta aaacgtcatt tttatttcaa
      661 ttagcggaaa ataaaaaaac cattccgcat taactttggc aagttggaat ggttaaaaaa
      721 atattttaga agccagcgtt tggcttgctt agacgacatg ttcgcgcatg tcgtcttatt
      781 tttacattta tataataaca cgcaattcaa taaaatgtct aatcaataaa cctcaattac
      841 attcaaattc aaaaactttt cacctaatat tcatcgctta aataaacatt acgtttaatt
      901 aaacagacat tgtcaatcgc gtctaatacc cctgctgtcc gaaaacaaaa ttattttgta
      961 ttcatattta actgttctct ttcctccctt ttcaacgcaa ctaaatttct tttaccgtta
     1021 gagccccaat ctttaatctt taattgttct accgctccat ttattaaatc ctttaaagag
     1081 taaaactgct aatagcaacg tgataataat atagattgcc aatgttaatg taactggtat
     1141 actcccttcg ataaacatat aaacgtaacg tgtagcatat gtgattggta aatagaacca
     1201 cgaatgatct ccaagcactt ctaatccaaa ataaacgtta aaaataaaca ttaaaactcc
     1261 gacaacaata gccattacat ctttaatgaa aatactaaaa ataaaaagta gcagtaatat
     1321 aattacattg aaaaacaatg atacgcctat aaacataagt gttattttca tatcatgtga
     1381 atgccacaat aaattaattg atgctaatag aatacatatg gctgtaatag tataagtaaa
     1441 aatcattgat gcatttaacc aatttagcct attagctttt cctaaaatat gattaaagtg
     1501 accaatattt tcttcaaaat tgataacttg atagacgttt atagaaatta atagcgatgt
     1561 aattgcatta aaactcgctg taaacaaact tatttgtcga ccattccata aatttacgtt
     1621 taaataccaa tttataaata atataaacaa tatggttaca ataatgggta caaatgtgag
     1681 cgcttgctta gaaaatttca acttacatga ctttaattcg ttaattatca ttttaatcgc
     1741 caacttttct tattatttaa aaatgttact atcgcgaaaa taatgatgct aacaataatc
     1801 gaaatatata aggcattgag atcattaaag tattttgaat tatggtttac aactaaacca
     1861 ttaggtagta taccaagcgt aataagcggg attctctgca ttatccccca cggcaacacc
     1921 caaaataaac tcttcaatgt taaaaacaag acacaaatga ctgataatac taagtttatt
     1981 aatattgata cgaacacacc aaagtatcga gttaataaaa agttgagcgg tatcaatggt
     2041 agagatacta catacatcaa caatattgtc accaataaca acatagcatt aaccggatgt
     2101 ggattaataa ttaggtcacc tatataagca ataataaata ctaaaaagca atgtaccaaa
     2161 aatgctattg ataaaatgaa aatctttgct cttatttct
//
LOCUS       DAMZKC010000260         2196 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.264, whole genome shotgun sequence.
ACCESSION   DAMZKC010000260 DAMZKC010000000
VERSION     DAMZKC010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.264"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..348
                     /locus_tag="POB55_002641"
     CDS             <1..348
                     /locus_tag="POB55_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HDI6501550.1"
                     /translation="FYQYKNCTTCKKAAKFLDEYGVSYEPIDIVQHTPTINEFKTIIA
                     NTGVEINKLFNTHGAKYRELDLKNKLQTLSDDEKLELLSSDGMLVKRPLAVMGDKITL
                     GFKEDQYKETWLA"
     gene            506..886
                     /gene="gcvH"
                     /locus_tag="POB55_002642"
     CDS             506..886
                     /gene="gcvH"
                     /locus_tag="POB55_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001831963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HDI6501551.1"
                     /translation="MAVPNELKYSKEHEWVKVEGNVATIGITEYAQSELGDIVFVELP
                     ETDDEINEGDTFGSVESVKTVSELYAPISGKVVEVNEELEDSPEFVNESPYEKAWMVK
                     VEISDESQIEALLTAEKYSEMIGE"
     gene            1065..1943
                     /locus_tag="POB55_002643"
     CDS             1065..1943
                     /locus_tag="POB55_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1963 domain-containing protein"
                     /protein_id="HDI6501552.1"
                     /translation="MLNIQDVSHLSKKEQKAYNRFVESVENGNLPVLPCIEMVLKEMK
                     EETLNQSKIGGMPFLKSFKDIPLDENNVPMVLLAQINLDNLPEQQELFPVKEGILQFW
                     ISSEDQMYGMSENLKGNNINSRLVYIKEPITDLSLENIQAHLKSLDADNEDIPFSGAF
                     SIEFRLSKQTITCTDYKYDEDVLALWNKVNPSFALKSMFGGYDELMEPVCNTFTAKEP
                     FNQLGGYPYFDQIDPRTNDQELKMYDRVLLQIDSTRDGNSSIIWGDLGIANILVKSTD
                     LEAMKFDDYMYSWDCS"
BASE COUNT          808 a          283 c          419 g          686 t
ORIGIN      
        1 ttttaccaat ataagaattg tacaacttgt aaaaaggcag caaagttttt agatgaatat
       61 ggcgtaagtt atgaaccaat tgatatcgtt caacatacac ctacaataaa tgaatttaaa
      121 acaataattg caaatacagg cgtagaaatt aataaattgt ttaatacaca cggcgcgaaa
      181 tatcgtgagc ttgatttgaa aaataaatta caaactttat cagatgatga aaagttagag
      241 ttgttatcat ctgatggtat gttagtaaag cgtcctctag cagtaatggg cgataagata
      301 acgttaggat ttaaagaaga tcaatataaa gagacttggt tagcgtaagt ataatgtaaa
      361 cgtttactaa atatctcgat atttagattc attacatgta aaatgaaata agctatacaa
      421 ttgttaattt ttataaatat agttgaatag catctagcct tatggcatca ttaatgatgt
      481 aaagattaat taggagggga ttctcttggc agtaccaaat gaattgaaat attcaaaaga
      541 gcatgaatgg gttaaagttg aaggtaatgt agcaacaatt ggaatcacag aatacgcaca
      601 aagcgagtta ggtgatattg ttttcgttga attaccagaa acagatgatg aaattaatga
      661 aggggatacg tttggtagcg tagaatcagt taaaactgta tcagaattat atgcaccaat
      721 ctctggtaaa gtagttgaag tcaacgaaga actagaagat agtcccgaat ttgtaaatga
      781 atctccatac gaaaaagcat ggatggtaaa agtagaaatt agtgatgaaa gtcagattga
      841 agctttatta acagctgaaa aatattcaga aatgattggt gaataatcac cgtgtaactc
      901 cttaatctaa gattgaggag tttattttta gtctgagtaa gtaaataata tgaaagaaaa
      961 tttattaggt actattattt ggagtattgc tacattttat tattcaagaa tgatgaaaat
     1021 aatgaattta gctattttaa aaataaaaat tgggggaagt taatatgcta aacattcaag
     1081 acgttagtca tctttctaaa aaggagcaaa aagcatataa ccgtttcgta gaatctgtag
     1141 aaaacggtaa tttaccagta ctaccatgta ttgaaatggt tctaaaagag atgaaagaag
     1201 aaacattaaa ccagagtaag attggtggaa tgccattttt aaaatctttt aaagatatac
     1261 cattagatga aaataatgta ccaatggtat tgttagcaca gattaatttg gataatcttc
     1321 cagaacaaca agaattattt cctgtaaaag aagggatatt gcagttttgg attagttcag
     1381 aagatcaaat gtatggtatg tctgaaaatt taaagggaaa caatataaac tcaaggcttg
     1441 tttatataaa agagccaatt acagatttat cactcgaaaa tattcaagcg catttgaagt
     1501 cattagatgc tgataatgag gatatcccgt tcagtggagc attttctata gaatttagat
     1561 tgtcgaaaca aactattaca tgtactgatt ataagtacga tgaggacgtg cttgcattgt
     1621 ggaataaagt caatccatcc ttcgcgctaa aatcaatgtt tggtggttat gatgaattga
     1681 tggaacctgt gtgtaacaca tttactgcta aggaaccatt taatcaactt ggtggttatc
     1741 catattttga ccaaatagat ccaagaacga acgatcaaga actgaaaatg tatgatagag
     1801 tcttactgca aattgattct acaagagatg gtaattcttc gattatatgg ggtgatttag
     1861 gtattgccaa tatcttagta aaatctactg accttgaggc tatgaagttt gatgattaca
     1921 tgtattcatg ggattgcagc taagagaatc ataaaaatat attactttaa taagtgtagt
     1981 atctatagat caatctaggg agtgggacag aaatgatatt ttcgcaaaat ttatttcgtc
     2041 gtcccacccc aacttgcatt gtctgtagaa attgagaatc caatttctct ttgttggggc
     2101 ccatccccaa cttgcattgc ctgtagaatt tcttttcgaa attctctgtg ttggggcccc
     2161 tgactagaat tgaaaaaagc ttgttacaag cgcatt
//
LOCUS       DAMZKC010000261         2187 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.265, whole genome shotgun sequence.
ACCESSION   DAMZKC010000261 DAMZKC010000000
VERSION     DAMZKC010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.265"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            15..>2187
                     /locus_tag="POB55_002644"
     CDS             15..>2187
                     /locus_tag="POB55_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding adhesin SdrG C-terminal
                     domain-containing protein"
                     /protein_id="HDI6501553.1"
                     /translation="MNNKKTVTNRKGMIPNRLNKFSIRKYSVGTASILVGTTLIFGLS
                     GHEAKAAEHTNGELNQSKNETTAPSENKTTEKVDSRQLKDNTQTATADQPKVTMSDSA
                     TVKETSSNMQSPQNATASQSTTQTSNVTTNDKSSTTYSNETDKSNLTQAKDVSATPKT
                     TTIKPRTLNRMAVNTVAAPQQGTNVNDKVHFSNIDIAIDKGHLNKDTGKTEFWATSSD
                     VLKLKANYTIDDSVKEGDTFTFKYGQYFRPGSVRLPSQTQNLYNAQGNIIAKGIYDST
                     TNTTTYTFTNYVDQYTNVSGSFEQVAFAKRENATTDKTAYKMEVSLGNDTYSEEIIVD
                     YGNKKAQPLISSTNYINNEDLSRNMTAYVNQPKNTYTKQTFVTNLTGYKFNPNAKNFK
                     IYEVTDQNQFVDSFTPDTSKLKDVTNQFNITYSNDNKTATVDLMNGQTSSNKQYIIQQ
                     VAYPDNTSTDNGKIDYTLDTDKTKYSWSNSYSNVNGSSTANGDQKKYNLGDYVWEDTN
                     KDGKQDANEKGIKGVYVILKDSNGKELDRTTTDENGKYQFTGLSNGTYSVEFSTPAGY
                     TPTTANAGTDDAVDSDGLTTTGVIKDADNMTLDSGFYKTPKYSLGDYVWYDSNKDGKQ
                     DSTEKGIKGVKVTLQNEKGEVIGTTETDENGKYRFDNLDSGKYKVIFEKPAGLTQTGT
                     NTTEDDKDADGGEVDVTITDHDDFTLDNGYYEEETSDSDSDS"
BASE COUNT          904 a          328 c          378 g          577 t
ORIGIN      
        1 aggagtatta ttaaatgaat aataaaaaga cagtaacaaa tagaaaaggc atgataccaa
       61 atcgattaaa caaattttcg ataagaaagt attctgtagg tactgcttca attttagtag
      121 ggacaacatt gatttttggg ttaagtggtc atgaagctaa agcggcagaa catacgaatg
      181 gagaattaaa tcaatcaaaa aatgaaacga cagccccaag tgagaataaa acaactgaaa
      241 aagttgatag tcgtcaacta aaagacaata cgcaaactgc aactgcagat cagcctaaag
      301 tgacaatgag tgatagtgca acagttaaag aaactagtag taacatgcaa tcaccacaaa
      361 acgctacagc tagtcaatct actacacaaa ctagcaatgt aacaacaaat gataaatcat
      421 caactacata tagtaatgaa actgataaaa gtaatttaac acaggctaaa gatgtttcag
      481 ctactcctaa aacaacgact attaaaccaa gaacattaaa ccgtatggct gtgaacacag
      541 tagcagcacc acaacaaggt acaaatgtta atgataaagt tcatttttca aatattgata
      601 ttgctattga taaaggacac ttaaataaag atactggcaa aacagaattt tgggcaactt
      661 caagtgatgt tttaaaatta aaagcgaatt acacaatcga tgattctgtt aaagagggcg
      721 atacatttac ttttaaatat ggtcaatatt tccgtccagg atcagtaaga ttaccttcac
      781 aaactcaaaa tttatataat gcccaaggta atattattgc aaaaggtatt tatgacagta
      841 caacaaacac aacaacgtat acttttacga attatgtaga tcaatataca aatgttagcg
      901 gtagctttga acaagtcgca tttgcgaaac gtgaaaatgc aacaactgat aaaactgctt
      961 ataaaatgga agtaagttta ggtaatgata catatagcga agaaatcatt gtcgattatg
     1021 gtaataaaaa agcacaaccg cttatttcaa gtacaaacta tatcaacaat gaagatttat
     1081 cgcgtaatat gactgcatat gtaaatcaac ctaaaaatac atatactaaa caaacgtttg
     1141 ttactaattt aactggatat aaatttaatc caaatgcaaa aaacttcaaa atttacgaag
     1201 tgacagatca aaatcaattt gtcgacagtt tcaccccaga tacttcaaaa cttaaagatg
     1261 ttactaatca atttaatatt acttacagca atgataataa gactgcaaca gttgatttaa
     1321 tgaatggtca aacaagtagt aataaacaat acatcattca acaagttgcg tatccagata
     1381 atacatcaac agataatgga aaaattgatt atactttaga cactgacaaa actaaatata
     1441 gttggtcaaa tagttattca aatgtgaatg gctcatcaac tgctaatggc gaccaaaaga
     1501 aatataatct aggtgactat gtatgggaag atacaaataa agatggtaaa caagatgcca
     1561 atgaaaaagg gattaaaggt gtttatgtca ttcttaaaga tagtaacggt aaagaattag
     1621 atcgtacgac aacagatgaa aatggtaaat atcagttcac tggtttaagc aatggaactt
     1681 atagtgtaga gttttcaaca ccagccggtt atacaccgac aactgcaaat gcaggtacag
     1741 atgatgctgt agattctgat ggactaacta caacaggtgt cattaaagac gctgacaata
     1801 tgacattaga tagtggattc tacaaaacgc caaaatatag tttaggtgat tatgtttggt
     1861 acgacagtaa taaagatggt aaacaagatt cgactgaaaa aggaattaaa ggtgttaaag
     1921 ttactttgca aaacgaaaaa ggcgaagtaa ttggtacaac tgaaacagat gaaaatggta
     1981 aataccgctt tgataattta gatagtggta aatacaaagt tatctttgaa aagcctgctg
     2041 gtttaactca aacaggtaca aatacaactg aagatgataa agatgccgat ggtggcgaag
     2101 ttgatgtaac aattacggat catgatgatt tcacacttga taatggctac tacgaagaag
     2161 aaacatcaga tagtgactca gattcag
//
LOCUS       DAMZKC010000262         2176 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.266, whole genome shotgun sequence.
ACCESSION   DAMZKC010000262 DAMZKC010000000
VERSION     DAMZKC010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.266"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(<1..558)
                     /gene="deoC"
                     /locus_tag="POB55_002645"
     CDS             complement(<1..558)
                     /gene="deoC"
                     /locus_tag="POB55_002645"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDI6501554.1"
                     /translation="MHSAKLIDHTLLKPESTRTQIDQIIDEAKAYHFKSVCVNPTHVK
                     YAAERLADSEVLVCTVIGFPLGASTTATKAFETEDAIQNGADEIDMVINIGALKDGRF
                     DDVQQDIEAVVKAAKGHTVKVIIETVLLDHDEIVKASELTKAAGADFVKTSTGFAGGG
                     ATAEDVKLMKDTVGADVEVKASGGVR"
     gene            873..1583
                     /gene="deoD"
                     /locus_tag="POB55_002646"
     CDS             873..1583
                     /gene="deoD"
                     /locus_tag="POB55_002646"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDI6501555.1"
                     /translation="MTKGTPHIQPNGVKIAKTVLMPGDPLRAKYIADNFLENVEQFND
                     VRNMFGYTGTYKGKEVSVMGSGMGIPSIGIYSYELYNFFDVDTIIRIGSCGALQENVN
                     LYDVIIAQAASTNSNYVDQYNIPGHFAPIADFELVTKAKNVADQIGATTHVGNVLSSD
                     TFYNADPTFNDAWKKMGILGIEMESAGLYLNAIHAGKKALGIFTVSDHILRDEATTPE
                     ERQNSFTQMMEIALEIAE"
     gene            complement(1704..2147)
                     /locus_tag="POB55_002647"
     CDS             complement(1704..2147)
                     /locus_tag="POB55_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HDI6501556.1"
                     /translation="MSNQQDVVKELNQQVANWTVAYTKLHNFHWYVKGPNFFSLHVKF
                     EELYNEASQYVDELAERILAVGGNPVGTLTECLEQSIVKEAAKGYSAEQMVEELSQDF
                     TNISKQLENAIEIAGNAGDDVSEDMFIGMQTSVDKHNWMFKSYLS"
BASE COUNT          663 a          410 c          342 g          761 t
ORIGIN      
        1 acgtacgcca cctgatgctt ttacttctac atcagcacct actgtatctt tcattaattt
       61 aacgtcttct gcagtcgcac cgccacctgc aaaacctgtt gaagttttaa cgaagtccgc
      121 accagccgct tttgttaatt cactcgcttt tacgatttcg tcatggtcca acaataccgt
      181 ctcaataatc acttttactg tgtgaccttt cgcagcttta accactgctt caatgtcttg
      241 ttgtacatca tcaaaacgtc catcttttaa tgcgccgatg ttgatgacca tgtcaatttc
      301 atctgcacca ttttgaatcg catcttctgt ttcaaatgct ttcgtcgcag ttgtcgatgc
      361 acctaatggg aatcctatta ccgtacaaac taacacctct gaatcagcta gtcgttctgc
      421 tgcatattta acatgcgttg gattcacaca tacagattta aaatggtatg ctttcgcttc
      481 atcgatgatt tgatcgattt gcgtacgtgt tgactcaggc ttcaataaag tgtgatcaat
      541 caattttgca ctatgcattt tctatctcct cctttatggt tgattataaa aatacggttg
      601 taaattagtt gattgagcgt caggttcatt taaatatcag gttagatgtt cgctttttat
      661 gtaaccgcat acatatacta ttacattaat tcatttccca taaacaaaca atacaattga
      721 acgtgatatc ttcattatga acgatgactt gacaacaagc taatcaggat tatattttta
      781 taattcttta attctatagt acaaaaattc gcaaaaaagg gaaacaaatg ttatcttaaa
      841 attattaatg aatattaagg agaagataac aaatgacaaa aggtacacca catattcaac
      901 caaatggagt aaaaattgct aaaacagtat taatgcctgg cgatccgcta cgcgcaaaat
      961 atattgctga taatttttta gaaaatgttg aacaatttaa cgatgtacgt aacatgtttg
     1021 gttacactgg tacatataaa ggtaaagaag tttctgtaat gggttctggt atgggtattc
     1081 caagtattgg tatttactca tatgagttat acaacttctt tgatgtagat acaatcattc
     1141 gtatcggttc ttgtggcgca ttacaagaaa atgttaactt atacgatgtt attattgcac
     1201 aagctgcatc aactaattca aattatgtag atcaatacaa tattccaggt catttcgcgc
     1261 ctatcgctga cttcgagtta gtaactaaag ctaaaaatgt cgctgaccaa atcggtgcta
     1321 ctacacacgt aggtaacgta ttatcttctg atacatttta caatgccgat ccaacattca
     1381 atgatgcttg gaaaaaaatg ggtattttag gtatcgaaat ggaatcagct ggtttatatt
     1441 taaatgcgat tcatgctggt aaaaaagcac ttggtatttt cacagtaagt gatcatattt
     1501 tacgtgacga agctactaca cctgaagaac gtcaaaattc atttacacaa atgatggaaa
     1561 tcgctttaga aatcgcagag taacttattt aaattgactt taattgctct ttaacaatgc
     1621 gattaaactc aaaaagccaa cacattctgg gcgtatcccc atttatgtgt tggcttttat
     1681 ttatattatt acttatctgt agattagctt aagtaagatt taaacatcca attatgttta
     1741 tctactgatg tttgcatacc tataaacata tcttctgata catcatcgcc agcattacca
     1801 gcaatttcga ttgcgttttc taattgtttt gagatatttg tgaagtcttg tgataattct
     1861 tcaaccattt gttctgcaga gtaacctttc gcagcttctt taacaattga ttgctctaag
     1921 cattcagtta atgtacctac agggtttcct cctaccgcta aaattctttc agctaattcg
     1981 tctacatatt ggcttgcttc attatataat tcttcaaatt taacgtgtaa tgagaagaag
     2041 ttaggtcctt tcacatacca gtggaaatta tgtagctttg tgtaagctac tgtccagttt
     2101 gctacttgtt gattcaattc ttttacaaca tcttgttgat tactcatatt taatacactc
     2161 cttaaaattg tctacg
//
LOCUS       DAMZKC010000263         2172 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.267, whole genome shotgun sequence.
ACCESSION   DAMZKC010000263 DAMZKC010000000
VERSION     DAMZKC010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.267"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            19..1806
                     /locus_tag="POB55_002648"
     CDS             19..1806
                     /locus_tag="POB55_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002470062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HDI6501557.1"
                     /translation="MSEFKRHQQTSINVQKQANLIWNVADILRGLYKPHEYGKVILPM
                     TVIKRLHDTLLKTRDKVLKTAENTQSINDVMRERLLKNASGYSFYNTSLYTFETLLAD
                     PANIESNFRAYLNGFSENMQDILNNFKFDVEITTMADNDVLFYVIQEFNKADAYLGPD
                     KMTSTDMGYVFEELVRKFSESYNEEAGAHFTSRDIIYLMTDLLLIEDKDTLFKEHVFK
                     TVYDQTMGTSQMLSAMTERIHDMNDTAEVATFGQELNPETYAISKADTMIRGGDPENM
                     ALGSTLTSDRFEGFTFDYCISNPPFGVDWKKDQKAVKAEHELGELGRFGVGLPRVSDG
                     QLLFQLNGISKLKETGRMAIIHNGSALFSGNPGAGESLIRQYVIENDWLEGIIQLPND
                     LFYNTGISTYIWIITKDKSPERQGKVQLVDASNMYEKRRKNIGEKRVDISEACREMIV
                     QAYGEFNDKEYYLGDGTVESKILKNESFGFTRVTIERPQRDENGDIVYKKNGSKSVDT
                     NLRDTEDIPLTEDINEYFEREILPFNQDAWMDRKKDKIGYEIPFTRLFYKYTPPEPSE
                     VISERIKQLEESIIKNFQALSGKEVDTNE"
     gene            1799..>2172
                     /locus_tag="POB55_002649"
     CDS             1799..>2172
                     /locus_tag="POB55_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDI6501558.1"
                     /translation="MSREMKYSGIEWIGYIPKYWTITKLKNIIDFISGYAFKSELFTI
                     SDNNKKVITIKSFNTKEIILDNLSYSNESLKFPTKYLLKNNDILFAMSGGTTGKNLLI
                     EQVDDLYYINQRVGIIRSSFSK"
BASE COUNT          801 a          338 c          410 g          623 t
ORIGIN      
        1 ttaaaggaga tatacataat gagcgaattt aaaagacatc aacaaacatc aattaatgtt
       61 caaaaacaag caaaccttat ttggaatgtt gcagacattt taaggggttt atacaaacct
      121 catgaatatg gaaaggttat cttaccaatg actgtcatta aacgtcttca cgatacatta
      181 ctaaaaacgc gagacaaagt tcttaagact gcagaaaata cccaatcaat aaatgacgta
      241 atgcgagaac gccttttgaa gaacgcttca gggtattcat tttataatac aagcttatat
      301 acttttgaaa cattactcgc agacccagca aatattgaat caaactttag agcttatctc
      361 aatggtttct ctgaaaacat gcaagatatt ctcaataact ttaagttcga cgttgaaatt
      421 acgacaatgg cagataatga cgtgctattt tatgtgattc aagaatttaa caaagcagat
      481 gcatatctag gtccagacaa aatgacaagc acagatatgg gatacgtgtt cgaggaactt
      541 gtacgaaaat tttcggaaag ctataacgag gaagcaggtg cacacttcac gagtcgagat
      601 atcatttatc tcatgactga tttattgctt atcgaagata aagatacttt gtttaaagaa
      661 catgttttca aaactgtcta cgaccaaaca atgggaactt cccaaatgct ctcagcaatg
      721 acagaacgta ttcacgatat gaatgatact gccgaagtag ctacctttgg tcaagagcta
      781 aatcctgaaa cttatgcgat ttcgaaagca gatacaatga ttcgtggggg agatcctgaa
      841 aacatggcgc ttggaagtac attaacgagc gaccggtttg aaggatttac atttgattac
      901 tgtatctcca acccaccatt tggtgtcgat tggaaaaagg accaaaaagc agtaaaagca
      961 gaacatgaac ttggtgaact cggtcgcttt ggtgttggat tgcctcgagt tagtgatgga
     1021 cagttgttat ttcaactcaa tggtatttca aaattaaagg aaacaggacg catggcaatt
     1081 attcacaacg gttctgcctt attctctgga aacccaggtg ctggcgagag tttaattcgt
     1141 cagtacgtaa ttgagaatga ttggttagag ggcataatcc aacttcctaa cgacttattc
     1201 tacaatacag gaatttcaac gtatatttgg attattacta aagataaatc tccagaacgt
     1261 caagggaaag tacaactcgt tgatgcgtcc aatatgtatg aaaaacgtcg taagaatatt
     1321 ggtgaaaaac gcgtagatat ttctgaagca tgtcgagaaa tgattgtgca agcttatgga
     1381 gaatttaatg ataaagaata ctatctagga gatggaactg tagagtcaaa aatacttaag
     1441 aatgaatcat ttggttttac tcgagtgact attgaacgac cgcaacgaga cgaaaacgga
     1501 gatatcgttt ataaaaagaa tggaagcaag agtgtagaca cgaaccttag agatacagaa
     1561 gatattccct taacagaaga tattaatgaa tattttgaac gtgaaatatt accatttaat
     1621 caagatgcat ggatggatcg taaaaaagat aagatagggt atgaaattcc atttactaga
     1681 ttgttttaca aatatacacc accagaacca tctgaagtta tttcagaacg tattaaacaa
     1741 ctcgaagagt ctattattaa aaacttccaa gcattatctg gaaaggaagt ggatacaaat
     1801 gagtagagaa atgaaataca gcggaattga gtggattgga tatataccaa agtattggac
     1861 tattactaag cttaaaaata ttattgactt tataagtggt tatgccttta aaagtgaact
     1921 tttcacgatt tcagataata ataaaaaagt aataacaatt aagtcattta ataccaagga
     1981 gattattctc gataaccttt cttactcaaa cgaatctctc aaatttccaa ctaaatattt
     2041 attaaaaaat aatgatattc tttttgctat gagtggggga acaactggaa aaaatttatt
     2101 gatagaacaa gtagacgatt tatattatat aaatcaaaga gtgggaatca taaggtcttc
     2161 ttttagtaag tt
//
LOCUS       DAMZKC010000264         2163 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.268, whole genome shotgun sequence.
ACCESSION   DAMZKC010000264 DAMZKC010000000
VERSION     DAMZKC010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.268"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(172..1179)
                     /locus_tag="POB55_002650"
     CDS             complement(172..1179)
                     /locus_tag="POB55_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HDI6501559.1"
                     /translation="MSYIIIVTIAVVIFSLIFDFINGFHDTANAVATAVSTRALTPKT
                     AILMAAVMNFIGALTFTGVAGTITKDIVDPFKLENGLVVVLAAILAAIIWNLATWFYG
                     IPSSSSHALIGSIAGAAIASEGSFGVLHYQGFTKIIIVLIVSPIIAFCVGFLMYSIFK
                     VIFKNANLTRANRNFRFFQIFTAALQSFSHGTNDAQKSMGIITLALIVANVQNDGSVE
                     PQLWVKFACATAMGLGTAIGGWKIIKTVGGNIMKIRPANGAAADLSSALTIFVASSLH
                     FPLSTTHVVSSSILGVGASNRAKGVKWSTAQRMIITWVITLPISALLAGLLFYILNLF
                     F"
     gene            complement(1195..1812)
                     /locus_tag="POB55_002651"
     CDS             complement(1195..1812)
                     /locus_tag="POB55_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HDI6501560.1"
                     /translation="MFSKKKDKFMVQLEEMVFNLDRAAIEFGKMDFNTHLDLKAYSDN
                     IKTYESHGDELVHQVITDLNQTFITPIEREDILSLCDAIDDVLDAIEETAAMFEMYSI
                     EYTDEYMAEFVDNIQKAVAEMKLAVGLLVDKKLSHMRIHSINIKEFETNCDGILRQSI
                     KHIFNSETDPITLIKIKDIYESMEEIADKCQIVANNFETIIMKNS"
BASE COUNT          744 a          383 c          304 g          732 t
ORIGIN      
        1 actgtttatg ttccatggaa taaatttata acttagaccc ctaattaaaa tgctttgtct
       61 tggtcattct acgtaaactc tataaaatat gttatctact tacataaaaa actgaacttc
      121 aataccacca tatgtttgtg atactgaagt tcagtttagt tttattttca attagaaaaa
      181 taagttaagt atatagaata gtaaacctgc taacaatgct gaaataggta atgtaatcac
      241 ccatgtaatg atcattcgtt gcgcagtgct ccattttaca cctttagctc ggttagaagc
      301 accaacacct aagattgatg atgacacaac gtgagttgtt gataatggga aatgtagcga
      361 tgatgcaaca aaaattgtta atgcagatga taaatcggcc gcagcaccat ttgctggacg
      421 tattttcata atattaccac ctacagtttt gataattttc cagccaccaa ttgcagtacc
      481 aagccccatt gctgtcgcac aggcaaattt tacccataac tgtggttcaa cgctgccatc
      541 attctgtaca ttagcaacaa ttaatgccaa cgtaataata cccattgact tttgcgcatc
      601 attcgtaccg tgagagaatg attgtaacgc tgctgtgaaa atttggaaaa atctaaagtt
      661 acgattcgct cttgttaaat ttgcattttt aaagataact ttaaaaattg aatacatcaa
      721 gaaaccaaca caaaatgcga ttatcggtga aacgattaat acaataataa tttttgtgaa
      781 accttggtaa tgtaacactc caaatgagcc ttcagatgcg attgctgcac ccgcaattga
      841 acctataagt gcatgtgaag acgaacttgg aattccgtaa aaccaagtag ctaaattcca
      901 aataatagcc gcaagtattg cagctaacac aacaactaat ccattttcca atttaaatgg
      961 atcgacaatg tctttagtaa tggtgcctgc aacgcccgta aatgttaaag cacctataaa
     1021 gttcatcact gctgccatta aaattgccgt tttaggcgtt aacgctctag tagatacagc
     1081 agtagctact gcattggctg tatcatggaa tccattgata aagtcaaata tcagcgagaa
     1141 aataactaca gctatagtga cgatgattat atatgacata aatatatact ccccttagct
     1201 atttttcata ataatagttt caaaattatt tgctacgatt tgacatttat cagcgatttc
     1261 ttccatgctt tcataaatat cttttatttt aattaaagtg attggatctg tttcgctatt
     1321 gaaaatatgt ttaattgact gtcttaaaat accatcacag tttgtttcaa attctttaat
     1381 attaattgaa tgaatacgca tatgtgataa ttttttatcg actaataagc cgacagcaag
     1441 tttcatttct gcaactgctt tttgaatgtt atcaacaaac tcagccatat attcatctgt
     1501 gtattcgatt gaatacattt caaacatagc tgccgtttct tcaattgcat ctaaaacatc
     1561 atcaattgca tcacataatg ataaaatatc ttcacgttca attggtgtga taaatgtttg
     1621 atttaaatca gtaattactt gatgtactaa ttcgtcacca tgtgactcat aagttttaat
     1681 gttgtctgag tatgctttta aatctaaatg tgtattgaaa tccattttac cgaattcaat
     1741 agcagcacga tccagattga aaaccatctc ttctaattga accataaact tatctttttt
     1801 cttactaaac atttaaaacc ctccatttaa gcgattgtca ccaatcacat tcagttataa
     1861 tttgtttcaa attaagacaa gtgaatttac aaactaatga tacaaatttg ttattatcaa
     1921 tcgtcagtat aattttagtg tactgatatt aatttcaaaa atgcctcaca gtaaacaatt
     1981 tactgtattt gcccttatat gaaaatagca caaaatttac aatcccacaa ttcaatttac
     2041 aaatttttaa caattccgaa acttaacttt tacataaaat ttaaatagta gtaggcattt
     2101 gttttgaaaa agttattgta taggcaattt tttatctata caactctaaa aattgtaaat
     2161 gga
//
LOCUS       DAMZKC010000265         2148 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.269, whole genome shotgun sequence.
ACCESSION   DAMZKC010000265 DAMZKC010000000
VERSION     DAMZKC010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.269"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(252..896)
                     /gene="trmB"
                     /locus_tag="POB55_002652"
     CDS             complement(252..896)
                     /gene="trmB"
                     /locus_tag="POB55_002652"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HDI6501561.1"
                     /translation="MRVRYKPWAEDYLKDHPELVDMDGQHAGKMTEWFDKTQPIHIEI
                     GSGMGQFITTLAAQNPHINYISMEREKSIVYKVLDKVKEMSLTNLKIICNDAIELNEY
                     FKDGEVSRIYLNFSDPWPKNRHAKRRLTYHTFLALYQQILNDEGDLHFKTDNRGLFAY
                     SLESMSQFGMYFTKINLNLHQEDDGSNILTEYEKKFSNKGSRIYRMEAKFHSQK"
     gene            complement(911..1702)
                     /locus_tag="POB55_002653"
     CDS             complement(911..1702)
                     /locus_tag="POB55_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HDI6501562.1"
                     /translation="MEQFYQLGWTLDSAGGASGEAYMAEQDGQKLFLKRNSNPFIAAL
                     SAEGIVPKLVWTKRIETGEVVTAQHWKNGRELSSNEMKQTRVAHLLKKIHNSRPLLSM
                     LKRMEMEPITPEIMLNKINASLSREVLTHHIVRKSLTYLEEHIPSLDSRFFTVVHGDV
                     NHNNWLLSDRDELFLVDWEGAMIADPAIDIGMLLYNYVPQQQWSEWLETYGVQESLNL
                     NKRMKWYTVIQSIGLVQWYEEQKRYKDMNTWLKFLNQVMNSNMFI"
BASE COUNT          680 a          400 c          286 g          782 t
ORIGIN      
        1 tattctttta aatcaaaggt atttagtata acattgttca taggcgcaat tgtctccttt
       61 agtttaggtt tggtcacttt aaattacaga ggcaattgcg cttttttata ttcaaaatca
      121 aaaaatcgga cagatgaaaa gtaaaatact ttttctatca gtccgattta ttatagaacc
      181 cttttactat agaaccatta tagtacgaaa aaacacttat gattgtatgt gtacataccc
      241 tcataagtgc tttatttttg tgaatggaat ttcgcttcca tacgataaat acgtgaccct
      301 ttattcgaaa atttcttttc atattctgtt agaatattac tgccatcgtc ttcttgatgt
      361 aaatttagat ttatttttgt aaaatacatt ccaaattgag acatactttc taaactgtag
      421 gcaaatagtc ctctgttatc agttttaaaa tgtaaatctc cttcatcatt taagatttgt
      481 tgatacaacg ctaaaaacgt atgatacgtt aaacgtcgtt ttgcgtgacg attttttggc
      541 catggatctg aaaagttcaa ataaatacgc gaaacttcgc cgtctttaaa atattcattt
      601 aattcaatgg catcattaca aataatcttt aaatttgtta aactcatctc tttaacttta
      661 tccaatactt tataaacgat acttttctca cgttccattg aaatatagtt aatatgagga
      721 ttttgagcag ctaatgttgt aataaactgc cccatacccg aaccaatttc aatgtgtatc
      781 ggttgcgttt tatcaaacca ttcagtcatt ttccctgcat gttgaccgtc catgtcaacc
      841 aattcaggat gatcttttaa ataatcttca gcccatggtt tgtatcgaac tctcatattt
      901 tattctcctc ttaaataaac atgttactat tcataacttg atttaggaat ttaagccaag
      961 tgttcatatc cttatatctt ttttgctctt cataccattg cacaagacct atagattgaa
     1021 ttaccgtata ccatttcata cgtttattta aattcaagct ctcttgaaca ccatatgttt
     1081 caagccattc agaccattgt tgttgtggaa catagttgta aagcagcatt ccgatatcaa
     1141 ttgccgggtc tgcgatcatt gcaccttccc aatcgactaa aaatagttca tctcgatcag
     1201 ataataacca attattatga ttcacatcac catgtacaac agtgaaaaaa cgtgaatcta
     1261 aactcggtat atgctcttct aaataggtta atgattttct cacaatatga tgtgttaaaa
     1321 cttctcttga taaagaggca ttaattttat taagcataat ctcaggagta ataggttcca
     1381 tttccatacg ctttaacata cttaataaag gtctagaatt gtgtatcttc tttaataaat
     1441 gtgcaactct tgtttgcttc atttcgtttg aagatagttc acggccattt ttccaatgtt
     1501 gtgctgtaac aacctcgcct gtttctatgc gcttcgtcca tactaatttg ggcacaatac
     1561 cttctgctga taatgccgca ataaatggat ttgaatttcg ttttaaaaac aacttttgtc
     1621 catcttgttc agccatatat gcttcgccag atgcaccacc tgcagaatca agtgtccacc
     1681 ctaattgata aaactgctcc aactcgtcca cctcactttc aattagaaaa tggctctaga
     1741 aataggtttt tcaagagcca tatattctaa tttataacac catactggta caaatattat
     1801 gtccagataa ttattgtaaa tcctcaacta atgcctacat tacacgacta aatttaaatc
     1861 gtaatgtctg tcattgacac catacattct atagtcactt acttgacata aaatgttacc
     1921 gtgtctaaaa ctacatgttt ttgtaacttt tgtaggcgat aaactctagt tttcaaaata
     1981 attgctatcc cattttcatg gttagcataa atttatgaac tgtaacattt acgtacttag
     2041 taaaatatga tgcacatcat atttgtaact catagaaaat tttataattt ttatcattat
     2101 atttcaactg aaaatgagaa acaaaatggc actttttact aatatgtg
//
LOCUS       DAMZKC010000266         2138 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.270, whole genome shotgun sequence.
ACCESSION   DAMZKC010000266 DAMZKC010000000
VERSION     DAMZKC010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.270"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(86..892)
                     /gene="yjbH"
                     /locus_tag="POB55_002654"
     CDS             complement(86..892)
                     /gene="yjbH"
                     /locus_tag="POB55_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease adaptor protein YjbH"
                     /protein_id="HDI6501563.1"
                     /translation="MAGELRIMENKSREDINLSPVSKIEIYSFFDPFSSDCFKLSAIL
                     SKLRIEYNQYIRIRHILNPSLKVLTKCQAQSTSNFDNIALAYKAAELQGRVRAERFIH
                     LMQNEIIPKRDIITESMICDCIQNAGIDLEVFKDDLQKSKLTESLKIDLHIAREMEIE
                     QAPSLVFFSEDVHEEGLKVEGLYPYHIYTYIINELMGKPIEKNLPPKLETYIQQQQLV
                     TMEELLTIYEWPEKLLNKELKKLAIQQKIEKLKYPDGDFWKSKMPKIKSK"
     gene            complement(915..1280)
                     /locus_tag="POB55_002655"
     CDS             complement(915..1280)
                     /locus_tag="POB55_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="truncated hemoglobin"
                     /protein_id="HDI6501564.1"
                     /translation="MTTTPYDIIGKEALYDMIDYFYTLVEKDERLNHLFPGDFAETSR
                     KQKQFLTQFLGGPNIYTEEHGHPMLRKRHMDFTITEFERDAWLENMQTAINRAAFPQG
                     VGDYLFERLRLTANHMVNS"
     gene            complement(1384..1977)
                     /locus_tag="POB55_002656"
     CDS             complement(1384..1977)
                     /locus_tag="POB55_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HDI6501565.1"
                     /translation="MATNHEIEFKQMITASIYNKLQEKYFKDSVLFKQINYYIDTPDF
                     KLKEHRSALRIRVKDNQYEMTLKTPAKVGLLEYNYIVDIKPEMNLIISNDNLPDDIRQ
                     IIVEQFGVKDTTLSILGALTTYRQETKYKGDLLVLDKSEYFDTTDYELEFEVKDYNQG
                     LQKFQSLLNELNLEHHQPLNKVQRFFKKKETLSNNIN"
BASE COUNT          650 a          316 c          322 g          850 t
ORIGIN      
        1 tacgttctaa taatttaatg ttgcaaaatt aagagtcagt aaaaagacct cgttacattt
       61 atggtgtacg aggtctttaa tttatttatt ttgatttgat tttaggcatt ttagatttcc
      121 agaaatcgcc atctggatat ttaagttttt caattttttg ttgaatggct aacttcttta
      181 actctttgtt taaaagtttt tctggccatt cataaatagt aagtaattct tccatcgtta
      241 caagttgttg ttgctgtata taagtttcta atttaggagg aagattcttt tcgataggtt
      301 tacccatcaa ttcattaatt atataagtat agatgtgata tgggtataat ccttcgactt
      361 ttaaaccttc ttcatgaaca tcttcactaa agaaaacgag agaaggggct tgttcgattt
      421 ccatttctct tgcaatatgc aaatcaattt tcaagctttc ggttagttta cttttttgta
      481 agtcgtcttt aaatacttct aaatcaatac ctgcattttg aatacagtca caaatcattg
      541 attctgtaat aatatctctt ttaggtatga tttcattttg cattaaatgt ataaatcgtt
      601 cggcacgtac acgaccttgt aactcagctg ctttataagc tagggcgatg ttatcaaagt
      661 tggatgtact ttgagcttgg catttcgtta atacttttaa cgaaggattt aatatatgtc
      721 tgatacgtat atattgatta tattcaattc ttaatttgga taagattgct gataatttga
      781 agcaatcgga gctaaatgga tcgaaaaatg aataaatttc gattttactt acaggtgata
      841 gattaatatc ttcacgactc ttattttcca ttattcgtaa ttctccagcc atgtttattc
      901 acctacaatt aattttagga attcaccata tgattagcag ttaatcttaa gcgctcaaat
      961 aaataatctc caacaccttg tggaaacgca gcgcgattaa ttgctgtctg catattttct
     1021 aaccatgcat ctctttcaaa ttcagtgatt gtaaaatcca tatgtctttt tcttagcata
     1081 ggatgtccgt gttcttcggt ataaatgttt ggaccgccca aaaactgtgt taaaaattgt
     1141 ttttgtttac gacttgtttc tgcaaaatct cctggaaaca ggtgattaag tcgttcatct
     1201 ttttctacaa gggtgtaaaa ataatcaatc atatcgtata acgcttcttt accaatgatg
     1261 tcatatggtg ttgttgtcat ttaatcaccc attttcaaaa atttactgtt acgaacttaa
     1321 gttaatatat aactaatata acatgatttt aaacatttga aagaaatatg catatttgcc
     1381 aatttaattt atattgtttg aaagtgtttc ttttttcttg aaaaaacgtt gaactttatt
     1441 taaaggttga tgatgttcga ggtttagttc gtttaataaa gattggaact tttgtaaacc
     1501 ttgattatag tctttaactt cgaactctaa ctcataatcc gtagtatcga aatactcact
     1561 tttatctaaa acgagtaaat cacctttata tttagtttct tggcgatatg tcgttaatgc
     1621 accaagtatt gataaagttg tatcctttac accaaactgt tcaactataa tttgacgaat
     1681 gtcatctgga agattgtcgt ttgaaataat caagttcatc tctggtttaa tgtcgacgat
     1741 atagttgtat tctaatagac caacctttgc tggtgtcttt aaagtcattt catattgatt
     1801 gtctttaact cttatgcgta gtgcagagcg atgttccttt aatttgaaat cgggtgtatc
     1861 aatatagtaa ttgatttgct taaaaagcac actgtcttta aaatatttct cttgcaattt
     1921 attatagatt gatgcagtta tcatttgttt aaattctatt tcatgatttg ttgccatgat
     1981 atgtatacac ctcgtatcaa attcaattta tcttaactat attatgaatg acaaagttga
     2041 attttaaaag caatttcctt tatctattat caatgttaat ttgaccatta aaaatagtgt
     2101 tcgtaagtgt tttgtattat tgaattgtgt taaaatgt
//
LOCUS       DAMZKC010000267         2068 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.271, whole genome shotgun sequence.
ACCESSION   DAMZKC010000267 DAMZKC010000000
VERSION     DAMZKC010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.271"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            442..756
                     /locus_tag="POB55_002657"
     CDS             442..756
                     /locus_tag="POB55_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDI6501566.1"
                     /translation="MTYKELATFLKVLSDSSRLEILDLLSCGELCACDLLAHFQFFQP
                     TLSYHMKALVKTNLVTTRKIGNKHLYQLNHNIFESVINNLSKVHTSNQRCICHNLKTG
                     EC"
     gene            756..2048
                     /gene="arsB"
                     /locus_tag="POB55_002658"
     CDS             756..2048
                     /gene="arsB"
                     /locus_tag="POB55_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite efflux transporter membrane subunit
                     ArsB"
                     /protein_id="HDI6501567.1"
                     /translation="MMTTLATLIFLVTLLFVLWQPKGLDIGITALTGAFIAVITGVVS
                     FSDVFEVTGIVWNATLTFVSVILISLILDKVGLFEWSAIHMLHASKGNGLKMFVYIIL
                     LGAIVAAFFANDGAALILTPIVLAMVKNIGFSKRAIFPFIIASGFIADTTSLPLIVSN
                     LVNIISADYFHIGFIRYFSRMIIPNLFSLLASIIVLWLYFRKAIPKTFDDNNIKHPKD
                     AINDLKLFKISWIVLVILLFGYLISEFTKIPVSIFTGIIAFIFLMLARKSNALNIKQV
                     IKGAPWNIVLFSIGMYIVVFGLRNAGITLILAKILEYISNYGLFSTILGMGFISAFLS
                     SIMNNMPTVLIDAIAIGQSNVHGMLKEGLIYANVIGSDLGPKITPIGSLATLLWLHVL
                     TQKDVKISWGTYFKTGIIITIPVLFITLIGLYLTLIIF"
BASE COUNT          668 a          316 c          311 g          773 t
ORIGIN      
        1 atttattaag gcaagctatt atttcacttt cacaaccatt aaaccattta aaacttttca
       61 tttaatatca accttagtgt ttgtgttaaa atatttttaa cgaggtgaat taaattgaaa
      121 tttatagcaa agatattaat ctcattagta attctattta tgatttttag tcgtaaaaac
      181 aaagcttctt aatcaatacc cttgattaca aaataaattc aactaaggtg attggccata
      241 cactcaagat atggctaatc acctttttca atgcaaaaca aagagtatat tttgcaaaat
      301 caatatctat aatttatctt taataccctt ttaatactaa aaccaaattt aaattacata
      361 gtcatatcgt gtaatatacc aaaggaacat agacattcat ctatatagat gataatctat
      421 ttaacgtcta ggaggtatgt tatgacgtat aaagaactag caacattttt aaaagtttta
      481 tcagattcaa gcagattaga aatactagat ttactttctt gtggagagtt atgcgcttgt
      541 gatttgttag cacattttca attctttcaa cctacactta gctatcatat gaaagcatta
      601 gtaaaaacca acttagttac gacacgaaaa atcggaaata aacatttata ccagcttaat
      661 cataatattt ttgagtccgt aattaataac ttgtcaaagg ttcatacctc taaccaacga
      721 tgtatttgtc ataaccttaa gactggtgaa tgctaatgat gacaacttta gcgacactca
      781 tttttctagt aactttatta tttgtattat ggcaacctaa aggcctagat attggcatta
      841 ccgcattaac tggtgccttt attgctgtta ttactggtgt tgtaagtttt tccgatgttt
      901 tcgaagtaac aggtattgtt tggaatgcta ctttgacttt tgtctcagtc attcttattt
      961 cattaatatt agataaagtt ggattattcg aatggtcagc tattcacatg cttcatgctt
     1021 caaaaggcaa tggtttaaaa atgttcgttt atatcatatt attgggtgcc attgttgctg
     1081 catttttcgc aaatgatggc gcagcgttaa tcttaacgcc tattgtatta gcgatggtta
     1141 aaaatatagg ttttagtaag cgggccatat tcccttttat tattgcgagt ggttttatag
     1201 ctgacacaac ttctttacct ttgatcgtga gcaatctagt gaatattata tctgctgatt
     1261 attttcatat aggattcatt cgatatttta gtagaatgat tatacctaat ttattctcac
     1321 ttttagcaag tattatagta ttgtggttat attttagaaa ggcgatacct aaaacgtttg
     1381 atgataataa tataaagcat cctaaagatg ccattaatga tttaaagcta tttaaaattt
     1441 catggattgt gctagttata ttacttttcg gctatcttat cagtgaattt actaaaattc
     1501 cggtatcaat tttcactgga atcattgctt ttatttttct aatgttggct cgtaaatcaa
     1561 atgctttaaa tattaagcaa gtcattaagg gcgcaccttg gaatatagta ttattttcaa
     1621 ttggtatgta tatcgtcgta ttcggcttaa gaaatgctgg cattacttta atattggcta
     1681 aaatattaga atatatttcc aattacggtc tatttagcac tattttggga atgggcttca
     1741 tttcagcgtt tttatcatca ataatgaata atatgcctac agttttaata gatgcgattg
     1801 ctattggtca atcaaatgtc catggcatgt taaaagaagg cctaatttat gcgaatgtta
     1861 tcggttctga tttaggtcca aaaattacac cgataggctc tctagctaca ttactgtggt
     1921 tacacgtctt aacacaaaaa gatgttaaga tttcttgggg cacatacttt aaaaccggta
     1981 tcatcattac aattccagta ctatttataa ccctcatagg gttgtatcta acacttatca
     2041 tattttaatt aataaaaaga cgctttct
//
LOCUS       DAMZKC010000268         2054 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.272, whole genome shotgun sequence.
ACCESSION   DAMZKC010000268 DAMZKC010000000
VERSION     DAMZKC010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.272"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(<1..182)
                     /locus_tag="POB55_002659"
     CDS             complement(<1..182)
                     /locus_tag="POB55_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002501561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501568.1"
                     /translation="MLEDNGDIRLLYQSIEELASVLGQSQFETKTVSLIFVQMDLRYS
                     VFEKVQTDFMKYLAHK"
     gene            complement(204..683)
                     /locus_tag="POB55_002660"
     CDS             complement(204..683)
                     /locus_tag="POB55_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501569.1"
                     /translation="MKITKAIGSLVLTAVLLTSVSPLTNANEIQTNNTKTAHQSYENI
                     NGVKINRNPNLPMNPETQQRIGGTKVVKTAIKLIINNRGKAKNVIQQVGGKKAATIFD
                     KGYSGIVKELKPLLKWSEVPANAVGDAVKRGAINAGLSKSDAGIISTVVKEAIAWLF"
     gene            1609..1941
                     /locus_tag="POB55_002661"
     CDS             1609..1941
                     /locus_tag="POB55_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501570.1"
                     /translation="MATLKDVKTGKKEILPSTAKDKNGKNVTLIYFEKDGKLGFYVQK
                     TQKERGVGKCVSGIAGGAVTGGTTLGLAGAGVGTVTIPVIGTVSGGVVGAVGGAVGGG
                     LTGGATFC"
BASE COUNT          686 a          291 c          370 g          707 t
ORIGIN      
        1 tttttatgtg ctaaatactt cataaaatca gtttgcactt tttcgaaaac actatatctt
       61 aagtccattt gtacaaatat taagctgaca gttttagttt cgaattgact ttgacccaaa
      121 actgaagcaa gttcttctat tgattgatat agcaatctta tatcgccgtt atcttcaagc
      181 atatgaaaca ctcctttaac attttagaat aaccatgcaa tggcttcttt gactacagtt
      241 gagattattc cagcatcact tttacttaga ccggcattta ttgcgcctcg tttaactgca
      301 tcaccgacag cgttagctgg tacttctgac cattttagca atggtttcaa ttcttttaca
      361 atacctgaat accctttatc aaaaatagtt gcagcttttt tgccaccaac ttgttgtatg
      421 acattttttg ctttgcccct attattaata attagcttta tagcagtttt aactactttt
      481 gttccgccaa ttcgttgttg tgtttctgga ttcattggca agttaggatt gcgattaatt
      541 ttcacaccat ttatattttc ataactttgg tgagcagttt ttgtattgtt tgtctgtatt
      601 tcatttgcgt tagttaaagg tgaaacagac gttaataaaa cggcagtcaa gactagtgaa
      661 ccaatagctt ttgtgatttt cattatacat ttatctcctt taatagtgaa tttgcaaaac
      721 caaatgtttg aaatataagt aaataatcat taaaaaaaca aagtgagtga aaggtcctaa
      781 ttcgtataac tagcatttaa taatttaatt aatcgttttc accaaaatag aaaatgccac
      841 ttaacacaat tattaaaagt aatgtaacta tgaaagaagt ccaagctaat acaagtgaac
      901 atattaaaag tactaatgaa gtgactgttt gtaagaaccc aaaaagtaaa gattccatta
      961 tataagcaaa ataaatagtt agtgaaaaaa ttgctacagc aagtaacatt tgccctatga
     1021 ttttcaaaat tccagttgtg tagccagtaa ctaataaatt tttcacatga acttgattac
     1081 catattgcca tcccgtttat aaaatagttt agaagagatt gctattaaat ttaattgttt
     1141 tgcataagtg tcagaatatt attcttaact gttaagagtc gttaatttat ttaaatctat
     1201 taagattgtt agagattatt atgcgctggg aaacgccact gttacagtgg aagaggaatt
     1261 tggataacta gatatgttaa tcgtaaatgt ggcgtcgtga aacacgactt caacatcttc
     1321 gatcaatcta tctttgagat tggtcgaaga ttgaaacata tgtaagaaaa cgacatagtt
     1381 tatggaaagc tcggtaagta gttgaaaaat caatctagat tgaaatcatg catatcgttt
     1441 aataaaattt ttcggagaaa taaaaactta ctaaaaacga aaaggtttat ttgatttatt
     1501 tacttgtata ttgtaaatgg ttaaaatgag tctatagatt atgtagagaa ctctagtatg
     1561 ccttttgagt atatgtggga ttctttcttt atataaaagg agttcggagt ggcaacacta
     1621 aaagatgtaa agactggtaa aaaggaaatt ttgccatcaa ctgcaaaaga taaaaatggt
     1681 aaaaatgtta cgttaattta ttttgaaaaa gacggtaaat taggttttta tgtgcagaaa
     1741 acacaaaaag aacgtggagt agggaaatgc gtttctggta tagcgggtgg cgcagtgaca
     1801 ggaggcacta ctttaggtct tgcaggtgca ggagtaggaa cagttactat tccagtaatt
     1861 gggacagtta gtggaggcgt agttggagct gttggtggtg ctgtcggcgg tggtctaacc
     1921 ggtggagcca cattctgcta atagtgtagg agtgatacca tgaaaaaatt ttttaatcta
     1981 tgggtatgtt cgttcttatt tttagtaata atatcatggg taataattag tataaaattt
     2041 ggtaatatca gcac
//
LOCUS       DAMZKC010000269         2039 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.273, whole genome shotgun sequence.
ACCESSION   DAMZKC010000269 DAMZKC010000000
VERSION     DAMZKC010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.273"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(440..1369)
                     /locus_tag="POB55_002662"
     CDS             complement(440..1369)
                     /locus_tag="POB55_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDI6501571.1"
                     /translation="MLLFYIIGVLVGMLIPIQTSVNSRLSLYTKSPFYTSFISFSVGT
                     ICLIILNIIINPEVFTIHFYNNQSFNYTWVVGGLLGVSFLTGNLLLLPKLGATLTVIA
                     TVAGQIIMGVIIDTFGLFGATIHDFNLIKAIGVLLLIVGIVIMNQFNKNNLLLTDQKY
                     LLFWLLLGFIFGFFPPIQTTINSALASHTHSPAFASLVSFTIGSITLLILTAIFNRSL
                     KLKTSHLKFGKLKPIYFTGGILGMAFVTANIILMPHMGAALTTLIGMFGQILMGILID
                     HFGLFGSPKIAMTSRKTIGLLCILTGIILLRLF"
     gene            complement(1564..1788)
                     /locus_tag="POB55_002663"
     CDS             complement(1564..1788)
                     /locus_tag="POB55_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499185.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HDI6501572.1"
                     /translation="MTKTLELSFKSSLDKPVKLQLPQLNNVVTEQVARDSMNALVDLN
                     IIKSNSGTITKVHSAQIIDKTTTVLFEDKK"
     gene            complement(1805..2008)
                     /locus_tag="POB55_002664"
     CDS             complement(1805..2008)
                     /locus_tag="POB55_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501573.1"
                     /translation="MSKINHITIVLSFTKVDANGKQTEFKRRFANINPDASNDQIKTF
                     SKLIERLTGETYNNIELIKSLSI"
BASE COUNT          763 a          288 c          302 g          686 t
ORIGIN      
        1 aaaaacacat taatttagtg attaaaacca atctatagca cgttaaagtg tatatacgtt
       61 attccgattg ataacgggta acaaataata gaaagtacga taattgatca tgcattaaag
      121 cacttttatt tttcaaactc gactcaatga attataaaaa gttgtgcatt ttgacttatt
      181 tttaattaat tgaataacca actatgaaat attagcacga ataacataat ttttgctatt
      241 aatttggtgt aaattattaa aaaataacat taatttaggt cttaagaatg agttgactat
      301 cgaatttgct aaattaaaat ttgttgtgtc aaagtatact acccaaatca acgcttttct
      361 aaagattttt agaccacaaa aaaatagctt atcactttta aaaataatta caagttaaaa
      421 tgataagcta aaagttaatt taaaataatc ttaaaagtat aatgcctgtc aaaatacata
      481 atagaccaat agtttttctg gatgtcattg ctattttagg tgaaccaaat aatccaaagt
      541 gatctatcaa tatgcccatt agaatctggc caaacatccc aataagtgtt gttaatgctg
      601 cacccatatg aggcattaag ataatgttag ctgttacaaa agccatacca agtataccgc
      661 cagtaaaata gataggcttt aatttaccga attttaaatg acttgttttt agttttaaag
      721 aacgattaaa aatagcggtt aaaatcaata gcgttattga cccaattgta aatgatacta
      781 atgatgcaaa ggctggtgaa tgagtatgac ttgctaaagc actattaatt gtcgtttgaa
      841 taggtggaaa gaaaccaaaa ataaatccta agagaagcca aaacagtaaa tacttttgat
      901 cagttagtaa taaattattc ttgttaaatt gattcattat gacgatgccg acaatgagta
      961 acaatactcc aattgcttta attaaattaa aatcatgaat tgtagcgcca aataagccaa
     1021 atgtatcaat aatgacaccc ataataattt gacccgcaac tgttgcaatt acagttaatg
     1081 ttgcacctaa ttttggcaat aacaataaat tgccagttaa aaagctaacc ccaagcaaac
     1141 caccgactac ccatgtgtag ttaaatgatt gattattgta aaagtgaata gtaaatactt
     1201 ctggattgat aatgatattt aaaataatta aacaaattgt tccaactgaa aatgaaatga
     1261 atgaagtata gaaaggcgat ttagtataca gtgatagtct tgaattgaca gatgtttgga
     1321 taggaataag cattccaact aggacaccaa tgatatagaa aagaagcata tgaataaagt
     1381 ctcactttcc aaagttaata ataaaatcat atgaatacat tatcattata gagaaaatat
     1441 acaactcatt tacaataatg ttgaaaaaaa caggacatta tcacaacaaa atcaccctag
     1501 acaatcatga tgaaagtcta gggtgttagt atttatacta aattaattgc gctttcttta
     1561 tatctatttc ttatcttcaa ataaaacagt tgttgtttta tcaataattt gagcagaatg
     1621 tactttggtg atagtaccac tatttgactt tataatattt aaatctacta atgcattcat
     1681 actatcacgt gcaacttgct cggtcactac gttgtttaat tgtggtaatt gcagttttac
     1741 aggcttgtct agtgaagatt taaaacttaa ttcaagtgtt ttagtcatga atatgtcctc
     1801 ctgattaaat tgataaagat ttgatgagtt cgatattgtt atatgtttct ccagtaagtc
     1861 gctcaataag tttgctgaat gttttaattt ggtcgtttga tgcatcaggg ttaatgttag
     1921 cgaatcgacg cttaaattct gtttgtttgc cgttagcgtc tactttagta aatgataata
     1981 caatagtgat gtggtttatt ttactcatat tttaaaacct cctttcacac tatatatcg
//
LOCUS       DAMZKC010000270         2039 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.274, whole genome shotgun sequence.
ACCESSION   DAMZKC010000270 DAMZKC010000000
VERSION     DAMZKC010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.274"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            44..228
                     /locus_tag="POB55_002665"
                     /pseudo
     CDS             44..228
                     /locus_tag="POB55_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499823.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            739..843
                     /locus_tag="POB55_002666"
     CDS             739..843
                     /locus_tag="POB55_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501574.1"
                     /translation="MFKVNYSILSYYPEYNIAVSWQRLREGKTIKSKI"
     gene            972..1223
                     /locus_tag="POB55_002667"
     CDS             972..1223
                     /locus_tag="POB55_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501575.1"
                     /translation="MLTLIKLKEDEQVIIYEYIPEDDISNGKGSVTFNKKDAEVIDFS
                     LSEIENEEYFMLYRNKSFSVVRDFIEKQEFPENYKIAWY"
     gene            1296..1502
                     /locus_tag="POB55_002668"
                     /pseudo
     CDS             1296..1502
                     /locus_tag="POB55_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002485350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
     gene            1698..>2039
                     /locus_tag="POB55_002669"
     CDS             1698..>2039
                     /locus_tag="POB55_002669"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HDI6501576.1"
                     /translation="MLKDAKENNDSNEVAYLLKDGKVTKVYGDQDSVSFAPGEKATEL
                     LFNSKPNSIVMLHNHPGQSSFSLTDLYLFIFNNSIKTLTIVTNKGQTKYLTKTKEYCK
                     STCIDCIKKYNK"
BASE COUNT          836 a          241 c          333 g          629 t
ORIGIN      
        1 cgaaaaaaag cagaaaacat ggtacagata tggagatgaa aaaatgtcag aatacaagaa
       61 aaaaataatt gaattaattg aaagtaattt aacaggatat gaaatttcta aaaaactgga
      121 gtttctcaat acgtactttc acaattaaga cagggcaaac gcgaagtaga taatctaacc
      181 ctgaatacaa cagaaaaatt atatgaatat gccaataaag ttttgtaatt taactaatgt
      241 ataaattaat caagctatgt ttatttgatt taactattaa taaaaatcat atggtgaatg
      301 gatattataa taattaaaat acaaaaatag tagattccaa tttgtgaaaa aggagtatgt
      361 cgtttcattt agaatttatt gcacgaaatt agcatcgaat aagggaaaat aagtgttaag
      421 ttttaaatga taaaaaagat tggaatggat cgtcttgaaa tgctcccttc aaagttttca
      481 ttttttcaat gtctacttcg aaggggcatt tccattatat tgttatagct ttttatattt
      541 gtataatgaa catataagtt taagaagatg agagtgaagg aaataaaaaa gctcaaatgt
      601 accaaattgt taatcttaat aaatctctac tttataaaga ttgaatggac attcgagcgt
      661 taatcagtca ggagggaatt tcactcctac aatttaataa taatacttgc ttcaccactg
      721 tacaaggagt gatttgttat gttcaaagtg aattattcga ttttaagtta ttatccagaa
      781 tataatatcg cagtaagttg gcaacgttta agagaaggaa aaacaataaa aagcaagatt
      841 taatactgct gcgtcatgag gcgcttgaac attatttgat gaataagtat aatttcaact
      901 atgattatgc acataaaatt gtatcaaaaa aatacgatta ttcaattttt ataaaaaaga
      961 aggtggatta aatgcttaca ttaataaaat tgaaagaaga tgaacaggtt ataatatatg
     1021 aatatatacc tgaagatgat ataagtaacg gtaaaggttc agtaactttt aataaaaaag
     1081 atgcagaggt tatagatttc tcattatctg aaatagaaaa tgaagaatat tttatgttat
     1141 atcgtaataa gtctttttct gtagtaagag actttatcga gaagcaagaa tttcccgaaa
     1201 attataaaat agcatggtat taatgcatcc tttctagaca gataaagaga aagtggtgct
     1261 atttttatat gcttttttaa ctatttactg aaatagcaga atcacaaaag ttaacatatt
     1321 taaaagaact tggtgaagat ggcgaatata aatatgttgc caaaatagat agtaaaacgt
     1381 ctaaactatg tcattcgctc aacggcaaag tatttaaagt taaagatatg ataccaggtg
     1441 tgaatgcgcc acctatgcat ccttggtgta gaagtaccac agtgccacat gttggcaatt
     1501 ggtgagacaa gttctttaaa gagcgtgaag gtaaatatca agtagaagat gacacaacca
     1561 aagatgaatt acaacaagct aaagtattag gaaagaaaat atatattact gatcaagcaa
     1621 ttgataaagt cagatatgtt gatattccaa cacataccaa agaagaaaat caatttatac
     1681 aagaacaaca taaagccttg cttaaagatg ccaaagaaaa caatgatagc aatgaagtag
     1741 cttatttatt aaaagatggt aaagttacaa aagtatatgg tgatcaagat agtgtatctt
     1801 ttgcaccagg ggaaaaagca acagaattgt tatttaacag taaaccgaat tcaattgtta
     1861 tgttacataa ccatcctggg cagtctagtt tttctcttac agacttgtat ttatttatat
     1921 ttaataattc tattaaaaca ctgacaattg ttacaaataa aggtcaaaca aagtacttaa
     1981 ctaagacaaa agaatattgc aaatcaactt gtattgattg tattaaaaaa tataataaa
//
LOCUS       DAMZKC010000271         1994 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.275, whole genome shotgun sequence.
ACCESSION   DAMZKC010000271 DAMZKC010000000
VERSION     DAMZKC010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.275"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(<1..1796)
                     /locus_tag="POB55_002670"
     CDS             complement(<1..1796)
                     /locus_tag="POB55_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDI6501577.1"
                     /translation="MNKTKGFTKYKKMRYMPGLDGLRAIAVLGIIIYHLNKQWLTGGF
                     LGVDTFFVISGYLITSLLLKEYDDTGIIKLKSFWIRRLKRLLPAVIVLLMVVGTATLL
                     LKSDNIIRVKHDIIAAIFYVSNWWYIAKDVNYFEQFSFMPLKHLWSLAIEEQFYIFFP
                     VILVTLLLTIKKRYKIGFIFWGVSIISLGLMMFIYSINGDHSRVYFGTDTRLQTLLLG
                     VILAFLWPPFKLKNDPPKVVKYVIDSIGSLSFIVLILLFFIINDETNWIYDGGFYLIS
                     ILTLFIIASVVHPSTWIAKIFSNPVLVFIGKRSYSLYLWHFAVISFVHSYYVDGQIPV
                     YVYFIDISLTIIFAELSYRFIETPFRKEGIKALNWRPSYIPQFIRMAIVVTLLIPFML
                     ILVGAFNKYGKDIIGEKANSFDTTIEDNYLMRIAPIDNIHIDGLVSEKKKESSDVYNN
                     IKPLLIGDSVMVDIGESFKSSVPKSRIDGKVGRQLYQTLPLVKANYSQYKKSSDQVVL
                     ELGTNGDFTVKQLDDLLNQFGKAKIYLVNTRVPRIYEANVNRLLADAAKRKSNVTLID
                     WNKRSQGHSEYFAPDGVHLEYKGVLALKDEIL"
BASE COUNT          710 a          354 c          246 g          684 t
ORIGIN      
        1 tttaatattt catcttttaa agctaggact cctttgtact ctaaatgtac accgtctggt
       61 gcaaaatatt cactatgtcc ttgtgatcgc ttattccaat caattaatgt gacattggac
      121 tttcgtttcg ccgcgtcagc taataatcga tttacatttg cctcataaat tcttggaaca
      181 cgtgtattaa ctaaataaat cttggctttt ccaaattgat taagtaaatc gtcgagctgt
      241 ttgacagtaa agtcgccatt tgtacctaat tctaatacga cttgatcaga tgatttttta
      301 tattgtgaat aattcgcttt aactaaaggt aaggtttgat acaattgacg ccctactttt
      361 ccatcaattc tagacttagg aactgatgac ttaaatgact caccgatatc aaccattact
      421 gaatcaccga ttaaaagagg tttaatatta ttatatacgt cggaagattc ctttttcttc
      481 tcacttacta agccatcaat gtgaatgtta tcaattggtg ctatccgcat taaataatta
      541 tcttcaatag tggtatcaaa gctattcgct ttttctccaa taatgtcttt accatattta
      601 ttgaatgcac ctactaaaat caacataaat ggaattaaca aggttactac aattgccatt
      661 cttataaatt gtggtatata agaaggtcgc caatttaaag ctttaatacc ttcttttcta
      721 aatggagttt ctataaagcg atatgatagc tctgcaaata taattgttaa acttatatct
      781 ataaagtaca catatacagg tatctgtcca tctacatagt aactatgtac gaaactaatt
      841 actgcaaaat gccataaata taaactataa gaccttttcc cgataaatac taacactgga
      901 tttgaaaata tcttcgctat ccatgtagat ggatgaacga cactggcaat aataaataac
      961 gttaatatgg atattaaata gaaaccacca tcatatatcc aattcgtctc atcattaata
     1021 atgaaaaata ataatataag tactataaat gataaactac ctatgctatc aataacatat
     1081 tttacaactt taggtggatc atttttcaat ttaaacggtg gccataaaaa agctaaaata
     1141 acacccagta acaatgtctg taatctagta tctgtaccaa aatacactcg tgaatgatcc
     1201 ccattaatac tgtagataaa catcattaac cctaaagaaa ttattgatac tccccaaaaa
     1261 ataaatccta ttttgtatcg ctttttaatt gttaacaata atgtaaccaa aataactggg
     1321 aaaaatatgt aaaactgttc ttcaattgct aaagaccata aatgctttaa tggcataaat
     1381 gaaaattgct caaaataatt aacatctttt gctatatacc accagtttga tacataaaat
     1441 atcgcagcaa taatatcatg tttaacccta atgatattat ctgattttaa taataaggtt
     1501 gctgtcccta caaccattaa taaaactatg actgctggta ataaacgttt taaacgacgt
     1561 atccaaaagc ttttcaattt aatgatacct gtgtcatcat actctttgag taataagctt
     1621 gtaattaaat aaccagagat cacaaaaaat gtatccacac ctaaaaagcc acctgtcaac
     1681 cattgcttgt ttaagtggta aataataatt cctagaacag cgattgccct caaaccatcg
     1741 agccctggca tatatctcat tttcttatac tttgtaaaac cctttgtttt gttcattttt
     1801 gcattcttcc cttttaaaac tgttcttctt agatgcttaa ttaaatttag ttatgctgtt
     1861 taaaagaata ttgaaatgca tatgtatatt attgaattac gacatcatca aaatcatatt
     1921 gactaaaata ctgttaaatt aaaaaaatta ccaatgatga ttcttacttc gaaatccaat
     1981 ttgtaatgca actc
//
LOCUS       DAMZKC010000272         1929 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.276, whole genome shotgun sequence.
ACCESSION   DAMZKC010000272 DAMZKC010000000
VERSION     DAMZKC010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.276"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(287..>1929)
                     /locus_tag="POB55_002671"
     CDS             complement(287..>1929)
                     /locus_tag="POB55_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HDI6501578.1"
                     /translation="SSSPENPNGKKYSPVFIYSAIVVAIVVLLGAFLPEQFNYVTNNI
                     KMWITEKLGWYYLILTTIIVFFCIFLIFSPIGKLKLGKPNDKPEFNTISWFAMLFSAG
                     MGIGLVFYGAAEPMAHFATPPTADPKTTEAYTEALRSTFFHWGFHAWAVYGVVALALA
                     YSQFRKGEPGLLSRTLRPLLGDKVEGPIGIFIDVLSVFATIVGVAVSLGMGALQINGG
                     LHYLFNVPNNTFVQAIIIIVVTILFIASAWSGLSKGIQYLSNLNIGLGTILMVAALIV
                     GPTVLILNMLTSSTGSLLNTFLFNSFDTAALNPQKREWMSSWTLYYWGWWLSWSPFVG
                     VFIARVSKGRSIREFISGVLLVPAIVSFVWFSVFGVLGIETGKKHKEIFDMTPETQLF
                     GVFNHVPFGIVLSLIALLLIASFFITSADSATFVLGMQTTFGSLNPSSMVKVVWGISQ
                     ALIAFVLLLAGGGNGAEALNAIQSAAIISAFPFSFVVILMMVSFYKDANQERKFLGLT
                     LTPNKHRLQEYIKSQQEDYESDILEKRQSRRNIEKKDN"
BASE COUNT          694 a          379 c          287 g          569 t
ORIGIN      
        1 attgatttat gtcccagcct gagttaattt tctataaaag tatatttaat ttgcgtttat
       61 accgtcaaac ttcactttag ctttgtcaaa cccctttcta ttaagttttc agaaataaac
      121 ctatcttaaa atataaaaaa atcgagaatt cgtagtttaa taacgaaatt ctcgttctta
      181 tccttttgaa tatactcaat tttccacaaa aacaaacaag tagtatatct gttctagcta
      241 ctagaatgac atactacttg ttattaaaat acttaactaa actttattag ttatcttttt
      301 tctctatatt tctacgtgac tgacgctttt caagaatgtc agattcataa tcttcttgtt
      361 gactcttgat atattcttgt aagcgatgtt tattcggagt caatgttaaa cctaggaatt
      421 tacgttcctg gttcgcatcc ttgtagaaac ttaccatcat gagtatgacg acaaaggaga
      481 atgggaatgc acttataatt gcagcacttt gaatcgcatt taaagcttca gcgccgttac
      541 cgccaccagc taataaaagt acaaatgcta ttaaggcctg tgaaattccc caaacaactt
      601 ttaccatact agatggattt aatgaaccaa atgttgtttg cattcctaat acaaatgttg
      661 ctgagtcagc agatgtaata aagaatgatg caattaataa taatgcaatc aacgataaaa
      721 caatgccaaa tggcacatga ttaaacactc caaatagctg tgtttcagga gtcatatcaa
      781 aaatttcttt gtgtttctta cctgtctcga tgcctaatac accaaagaca ctaaaccaaa
      841 caaaactaac aattgctgga actagcaaga caccagaaat gaactctcta attgaacgtc
      901 cttttgaaac tcgtgcaata aacactccaa cgaatggact ccaacttaac caccaacccc
      961 agtaataaag tgtccatgaa gacatccatt cacgtttttg aggatttaaa gctgctgtat
     1021 caaaactatt aaacaagaat gtgtttagta aactacccgt agagctagtt aacatattta
     1081 aaataagaac agtcggtcca acaattaaag cagctaccat taaaatagta cctaaaccaa
     1141 tgttcaagtt acttaagtat tgaatacctt tacttaatcc agaccatgca cttgctataa
     1201 ataagatagt aacaacaatg atgataatcg cttgtacaaa cgtattgttt ggaacattga
     1261 acaagtaatg taaaccacca ttaatttgta gagcacccat acctaacgaa acggctaccc
     1321 caacgattgt cgcaaataca gataaaacgt caataaaaat cccaatagga ccttctactt
     1381 tatcacctaa aagaggacgt aaagttctag ataataaacc tggttcacct ttacggaatt
     1441 gcgaatatgc caacgctaac gcaacaacac cataaacagc ccaagcatgg aatccccaat
     1501 ggaaaaatgt tgaacgtaga gcttcagtat aagcttcagt agttttggga tctgctgtag
     1561 gtggcgtagc aaagtgcgcc atcggttcag ctgcaccata aaacaccaaa cctatcccca
     1621 taccagcact aaacaacata gcaaaccatg aaattgtatt aaactcaggt ttgtcatttg
     1681 gtttacctag tttaagtttt ccaataggac taaaaataag gaatatacag aagaacacga
     1741 taatcgtagt aagaataaga taataccaac ctaacttttc tgtaatccac attttaatat
     1801 tattggtaac atagttgaat tgttcaggta aaaatgcacc aagtaatacg actatagcaa
     1861 caacaattgc actatagatg aagactggtg aatacttctt tccatttgga ttctctggtg
     1921 aagaagaat
//
LOCUS       DAMZKC010000273         1921 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.277, whole genome shotgun sequence.
ACCESSION   DAMZKC010000273 DAMZKC010000000
VERSION     DAMZKC010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.277"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..181
                     /locus_tag="POB55_002672"
     CDS             <1..181
                     /locus_tag="POB55_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="superantigen-like protein"
                     /protein_id="HDI6501579.1"
                     /translation="LIKNKKLYNGEFNKGQIKITADGNNYTIDLSKKLKLTDTNRYVK
                     NPKNAQIEVILEKSN"
     gene            276..1001
                     /locus_tag="POB55_002673"
     CDS             276..1001
                     /locus_tag="POB55_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL13"
                     /protein_id="HDI6501580.1"
                     /translation="MNNNITKKIILSTTLLLLGTASTQFPNTPINSSSEAKAYYINQN
                     ETNVNELTKYYSQKYLTFSNSTLWQKDNGTIHATLLQFSWYSHIQVYGPESWGNINQL
                     RNKSVDIFGIKDQETIDSFALSQETFTGGVTPAATSNDKHYKLNVTYKDKAETFTGGF
                     PVYEGNKPVLTLKELDFRIRQTLIKSKKLYNNSYNKGQIKITGADNNYTIDLSKRLPS
                     TDANRYVKKPQNAKIEVILEKSN"
     gene            1109..1825
                     /locus_tag="POB55_002674"
     CDS             1109..1825
                     /locus_tag="POB55_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL12"
                     /protein_id="HDI6501581.1"
                     /translation="MSKNITKNIILTTTLLLLGTVLPQNQKPVFSFYSEAKAYSIGQD
                     ETNINELIKYYTQPHFSFSNKWLYQYDNGNIYVELKRYSWSAHISLWGAESWGNINQL
                     KGRYVDVFGLKDKDTDQLWWSYRETFTGGVTPAAKPSDKTYNLFVQYKDKLQTIIGAH
                     KIYQGNKPVLTLKEIDFRAREALIKNKILYNENRNKGKLKITGGGNNYTIDLSKRLHS
                     DLANVYVKNPNKITVDVLFD"
BASE COUNT          777 a          283 c          272 g          589 t
ORIGIN      
        1 attaataaaa aataaaaagt tatataacgg agaatttaat aaaggtcaaa ttaagataac
       61 tgctgatgga aataattaca cgattgattt aagtaaaaag ttaaaattaa ctgacacaaa
      121 ccgttatgtt aaaaatccta aaaatgcaca aattgaagtc atactcgaaa aatctaacta
      181 acctattacc ttttgtaaat gcggataatt tcaattatct aattaacccc tttttataat
      241 taaacattcc aacaatactc aaaggagaaa ttcgaatgaa caataacatc acgaaaaaaa
      301 ttattttatc aacaacattg ttactattag gtacagcatc tacacaattt cctaatacac
      361 ctatcaattc ttcatctgaa gcgaaagctt attatataaa tcaaaacgaa actaacgtta
      421 atgagttaac taaatattac tcgcaaaaat atttaacctt ctctaacagt acgttatggc
      481 aaaaagataa cggtacgatt catgcaacgt tgttacagtt ttcttggtat agtcatattc
      541 aagtttatgg acctgaaagt tggggcaata tcaaccaatt aagaaataaa agcgttgata
      601 tttttggcat aaaagaccaa gaaaccattg attcttttgc attatctcaa gaaacgttta
      661 ctggtggtgt tactcctgca gcaacatcta acgataaaca ctataaactg aatgtgacat
      721 ataaagataa agcagaaacg tttactggcg gatttccagt ttatgaaggc aataagcctg
      781 ttttaacttt aaaagaatta gattttcgta ttcgtcaaac attaattaaa agtaaaaaat
      841 tatataataa ttcttataat aaaggacaaa ttaaaataac aggtgcagac aataactaca
      901 caatagattt aagtaaaagg ttgccatcaa ctgatgcaaa tagatatgtt aaaaaacctc
      961 aaaatgcaaa aattgaagtt atcctcgaaa aatcaaacta acaataataa tggagttaat
     1021 aaaaataatc gcaaatacta tattgacttc gctcacattt aaatttctta ttcctcgtat
     1081 catgattcct ctgaaaggag atgttctaat gagtaagaac atcacgaaaa atataatttt
     1141 aacgacaaca ttattactat taggtactgt attacctcaa aatcaaaaac cagtatttag
     1201 tttttactct gaagctaaag cttatagcat tggtcaagat gaaactaaca tcaatgaatt
     1261 aattaaatat tacacacagc ctcatttttc attttcaaat aaatggctat atcaatatga
     1321 taatggaaac atttatgttg aacttaagag atattcatgg tcagcacata tatctttatg
     1381 gggcgctgaa agttggggaa atattaatca gttaaaaggt cgttacgtag atgtgtttgg
     1441 actaaaagac aaagatactg atcagttatg gtggtcttat agagagacat ttacaggtgg
     1501 cgttacacca gccgcaaaac cttctgataa aacttataat ctttttgtgc aatacaaaga
     1561 taaactacaa acgattattg gtgcgcataa aatataccaa ggcaataaac cagtattaac
     1621 attgaaagaa atcgatttcc gtgcacgaga agcgttaata aaaaataaaa tattatataa
     1681 cgaaaatcgt aataaaggta agcttaagat caccggtggc ggtaataact acactattga
     1741 tttaagcaaa agattacatt cagatctagc aaatgtttat gttaaaaatc ctaataaaat
     1801 aactgttgac gtcctctttg attagtatat gaaggtgact tatacttcat gcactttaat
     1861 tccaaatcag attatttaaa tgataatttt taaagtgtat gatgtatata ataggtaaaa
     1921 t
//
LOCUS       DAMZKC010000274         1914 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.278, whole genome shotgun sequence.
ACCESSION   DAMZKC010000274 DAMZKC010000000
VERSION     DAMZKC010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.278"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            109..897
                     /locus_tag="POB55_002675"
     CDS             109..897
                     /locus_tag="POB55_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDI6501582.1"
                     /translation="MNKVTINPQIQLTYQIEGKGDPIILLHGLDGNLAGFEDLQHQLA
                     SSYKVLTYDLRGHGKSSKSESYDLNDHVEDLKILMEKLNIHEAHILGHDLGGVVAKLF
                     TDKYAYRVKSLTTIASKKDDLIHSFTQLLIQYQDDIAGFNKSEAYILLFSKLFRNQEK
                     TMKWYQKQRIYSIKSEDDSAVAIRSLILHKDEPMYLKKRTCVPTLLINGEHDPLIKDK
                     NHFKLEAHFLNVTKKIFEHSGHAPHIEEPEAFMNYYLNFLKSVS"
     gene            complement(1147..1521)
                     /locus_tag="POB55_002676"
     CDS             complement(1147..1521)
                     /locus_tag="POB55_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SarA family transcriptional regulator"
                     /protein_id="HDI6501583.1"
                     /translation="MAITKINDCFELLSMVTYADKLKSLIKKEFSISFEEFAVLTYIS
                     ENKEKEYYLKDIINHLNYKQPQVVKAVKILSQEDYFDKKRNEHDERTVLILVNAQQRK
                     KIESLLSRVNKRITEANNEIEL"
BASE COUNT          678 a          241 c          292 g          703 t
ORIGIN      
        1 ttggaataac ttttggtaat ttataaataa tttaaaaaaa ttgtttataa aatggaagcg
       61 tatatagaat gaaggttggg tatatagttt attgagggag gtgtcacaat gaataaagtc
      121 acaattaatc ctcaaatcca attaacttat caaattgaag gtaaagggga tcctataata
      181 ttacttcatg gattggatgg taatttagct ggatttgaag atttgcaaca tcaactagca
      241 tcatcatata aagtacttac ttacgattta agaggtcatg gcaagtcttc taaaagtgaa
      301 tcatacgatt taaacgatca cgttgaggat ttaaaaattc taatggagaa gttaaatatt
      361 catgaggcac atattctagg acatgattta ggtggggtag ttgctaagtt atttacagat
      421 aaatatgctt atcgtgtaaa atcattaact accattgcat cgaagaaaga tgacttaata
      481 cacagcttta ctcaattgtt aatacaatat caagatgata tagcgggttt taataagtct
      541 gaagcgtata ttcttttatt ttctaaattg tttagaaatc aagagaagac gatgaaatgg
      601 tatcaaaaac aaagaatata tagcattaag tctgaggatg atagtgcggt ggcaattcgt
      661 tcattaattt tgcataaaga tgaacctatg tatttaaaaa aacgtacatg tgtacctact
      721 ttgttaatta atggggaaca tgatcctttg attaaagata agaatcattt taaattggaa
      781 gcgcattttt taaatgttac gaaaaaaatc ttcgaacatt caggacatgc accgcatatt
      841 gaagaaccag aagcatttat gaattattat ttaaattttt taaaaagcgt atcataatat
      901 gtgatatata aacctagggc ataaagtctt taggcaatgt gaaaaagctg attactattc
      961 attatttgat agaaatcagc tttttttgaa atgtatttga tatatactgc tcgttatgcg
     1021 gctatcttcc ttatattaag tgccattagt gcaaaacctc ttaacattta caaaaaaaga
     1081 cataaaaaaa ggaagtttaa tagaatgtat catctatcaa acttcaccaa attgcgctaa
     1141 acaaaattat agttcaattt cgttgtttgc ttcagtgatt cgtttattta ctcgactcaa
     1201 taatgattcg atttttttac gttgttgtgc attaacaaga attaatacag ttctttcatc
     1261 atgctcatta cgttttttat cgaagtaatc ttcttgagat aaaattttaa ctgctttaac
     1321 aacttgtggt tgtttgtagt ttaaatgatt aataatatct ttaagatagt attctttctc
     1381 tttgttttcg ctgatgtatg tcaatacagc gaattcttca aagctaattg aaaattcctt
     1441 tttaattaaa ctttttaatt tgtcagcata agtgaccatt gataacaact caaagcaatc
     1501 attgattttt gtaattgcca tgtttaaaac ctccctattt gatgcatctt gctcgataca
     1561 tttgcccgat aatatattga tatctaatct ttatttatta tagatatgtt agtcataatt
     1621 ttgcattaaa taagttttat taaatatatt taatgctcta ttatttagtt aattataact
     1681 aattaaaaat gagaagtaaa caaaaaagtg tttataaaac aaattattca ttagacacag
     1741 tgattgtatt tctgggttag catttggttt agtcaaaaat atcagcattt tttataattt
     1801 accttaattt aatcgccaat acaaggtaat ttatttaatg aaacgattta gcgcaattaa
     1861 atgtttcgat attcaatgtg tgtctaaaga gattctttgt tatagcttaa ctct
//
LOCUS       DAMZKC010000275         1913 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.279, whole genome shotgun sequence.
ACCESSION   DAMZKC010000275 DAMZKC010000000
VERSION     DAMZKC010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.279"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(115..1422)
                     /gene="brnQ"
                     /locus_tag="POB55_002677"
     CDS             complement(115..1422)
                     /gene="brnQ"
                     /locus_tag="POB55_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDI6501584.1"
                     /translation="MKKQVIISGLMLFSLFFGAGNLIFPPMLGHTAGQNMWIGMLGFA
                     LTGILLPFITVIVVAFYDEGVESVGNRIHPWFGFIFAVVIYMSIGAFYGIPRAANVAY
                     EIGTRHILPVHNQWTLIIFAAIFFAIVYWISLNPSKIVDNLGKLLTPLLLLMVSLLSI
                     AVIFNPESALSAPKDKYITHPFISGSLEGYFTMDLVAALAFSVVIVNGYKFKGLTDRM
                     KILKYVCFSGLIAAILLGMIYFALAYVGASTAPGNFKDGTDILTYNSLRVFGSFGNLV
                     FGMTVILACLTTCIGLVNACATFTKKHVPKFSYKIFALIFSIIGFLFTTLGLEMILKI
                     AVPLLTLIYPVSIALVLISFANMFSTFRFSWAYRLATVITLIISILQILNSFNLLHGV
                     ILKSFMMLPLADIDLAWLVPFMLFAIIGFIIDVFIRRPKQATT"
BASE COUNT          713 a          297 c          335 g          568 t
ORIGIN      
        1 atcatttatt ttaaaataat taatcgtcag ttagtatcaa tgaataaata cagattttca
       61 tacaaaaaaa cctagtaatg atgcgacgat cattactagg agattatgcg tctattaagt
      121 tgtagcttgt ttcggacggc gtataaatac atcgattatg aaaccgataa tagcaaagag
      181 catgaatggt acaagccaag ctaaatcgat atctgctaaa ggtaacatca taaacgattt
      241 caaaataaca ccgtgtaata agttaaaact atttagtatt tgtaaaattg aaataatcaa
      301 tgtaataaca gttgcgagtc gataggccca actgaatctg aatgtgctaa acatgttagc
      361 aaatgatatc agtacaagtg caatagacac gggatatatt aatgtcaata atgggacagc
      421 aatttttaaa atcatttcta aaccaagtgt tgtaaataag aaccctatga tagagaaaat
      481 aagtgcgaat attttataag aaaacttagg tacgtgtttc ttagtaaatg tggcgcaagc
      541 attgacgagt cctatacatg ttgttaggca tgcaaggata accgtcattc caaatacgag
      601 gttaccgaac gaaccaaata ctcgtaatga gttgtacgtc aatatatctg taccatcttt
      661 aaagtttcct ggagctgttg atgccccaac gtatgcaagt gcaaagtaaa tcattccaag
      721 taatatggct gcaataagac ctgaaaagca gacatatttt aaaattttca tgcgatctgt
      781 gaggccttta aacttatagc cattgacaat gacaacggaa aaagctaacg cagcaacaag
      841 atccattgta aaatagcctt ccaaacttcc tgaaatgaaa ggatgtgtta tatatttatc
      901 cttaggtgca cttagtgcag attcagggtt gaaaatgaca gcaatactta atagagagac
      961 cattaatagt aataacggtg ttaataattt acctaaatta tcaacgattt tcgatggatt
     1021 taaactaatc cagtaaacga tggcaaaaaa gattgctgcg aatataatta aagtccattg
     1081 gttgtgcaca ggtaaaatgt gtcttgtacc aatttcgtac gcgacatttg cagcacgtgg
     1141 aataccgtaa aatgctccga tagacatgta aatcacgaca gcaaaaataa acccgaacca
     1201 tggatgtata cgattgccta cactttcaac accttcatca taaaatgcaa caacaataac
     1261 agtaataaag gggagtaata tgcctgtaag agcaaagcct agcataccaa tccacatatt
     1321 ttgacccgct gtatggccaa gcatgggcgg gaatattaaa tttccggctc caaaaaatag
     1381 tgaaaataac atgaggcccg aaataataac ttgttttttc aatgtaattt ccttcctaaa
     1441 actagatatt cataataatt taaaaaatct gaaaaataaa aacgttctta ctttatcttt
     1501 aaatgacaat actatgattc tattatttta taaaaaagat tgcaataata acgtcttgct
     1561 attattggat aatgacaatt aaagtgtcgt ttttatgaat taaaataagt gaaaataagt
     1621 tggaaatacg ttggagcgtt gtgattttta tgagaaattg tatgagtgag gactgctatt
     1681 tataaaaggt aaactatgga tgagtttata gttgttccaa taggtaaata agagatagca
     1741 cactgtacac ataatgatac gtggatgagt gaatatgtct gataggaagg cctagtcact
     1801 caaatgaatt taagtttatt acgtgataaa tcacaaatct ctctcatgtg ataggtctcc
     1861 cattaaatca tgcttatatg aaatgttcac atatttgtta gcttttcaag aaa
//
LOCUS       DAMZKC010000276         1907 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.280, whole genome shotgun sequence.
ACCESSION   DAMZKC010000276 DAMZKC010000000
VERSION     DAMZKC010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.280"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..112
                     /locus_tag="POB55_002678"
     CDS             <1..112
                     /locus_tag="POB55_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501585.1"
                     /translation="ANQRLIFTNSIVDWFYLCMNEQLFDIIKNINDFESI"
     gene            276..1832
                     /locus_tag="POB55_002679"
     CDS             276..1832
                     /locus_tag="POB55_002679"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HDI6501586.1"
                     /translation="MSITEKQRQQQAELHKKLWSIANDLRGNMDASEFRNYILGLIFY
                     RFLSEKAEQEYADALSGEDITYQEAWADEEYREDLKAELIDQVGYFIEPQDLFSAMIR
                     EIETQDFDIEHLATAIRKVETSTLGEESENDFIGLFSDMDLSSTRLGNNVKERTALIS
                     KVMVNLDDLPFVHSDMEIDMLGDAYEFLIGRFAATAGKKAGEFYTPQQVSKILAKIVT
                     DGKDKLRHVYDPTCGSGSLLLRVGKETQVYRYFGQERNNTTYNLARMNMLLHDVRYEN
                     FEIRNDDTLENPAFLGNTFDAVIANPPYSAKWTADSKFENDERFSGYGKLAPKSKADF
                     AFIQHMVHYLDDEGTMAVVLPHGVLFRGAAEGVIRRYLIEEKNYLEAVIGLPANIFYG
                     TSIPTCILVFKKCRQQDDNVLFIDASNDFEKGKNQNHLSDAQVERIIDTYKRKATIDK
                     YSYSATLQEIADNDYNLNIPRYVDTFEEEAPIDLDQVQQDLKNIDKEIAEIEQEINAY
                     LKELGVLKDE"
BASE COUNT          685 a          334 c          381 g          507 t
ORIGIN      
        1 agccaatcaa cgattgattt tcaccaactc aattgttgat tggttttatt tatgtatgaa
       61 tgaacaactt tttgacatca ttaagaatat aaatgatttt gaaagcattt gaaagctaca
      121 acatttctat aaaatttttc aataacaatt gcgccactaa aactcaaaat ttccaccacc
      181 aacatccaaa ttatcaacat cgcaacataa ccaaatgtta taataaatct attacacaaa
      241 gagataaatt acttatgcaa aggcggagga atcacatgtc tattactgaa aaacaacgtc
      301 agcaacaagc tgaattacat aaaaaattat ggtcgattgc gaatgattta agagggaaca
      361 tggatgcgag tgaattccgt aattacattt taggcttgat tttctatcgc ttcttatctg
      421 aaaaagccga acaagaatat gcagatgcct tgtcaggtga agacatcacg tatcaagaag
      481 catgggcaga tgaagaatat cgtgaagact taaaagcaga attaattgat caagtcggtt
      541 acttcattga gccacaagat ttattcagtg cgatgattcg tgaaattgaa acgcaagatt
      601 tcgatataga acatctagcg acggcgattc gcaaagttga aacatctaca ttaggtgaag
      661 aaagtgaaaa tgactttatc gggctgttca gcgatatgga tttgagttca acgcgactag
      721 gtaacaatgt caaagaacgt actgctttaa tctctaaagt catggttaat cttgacgact
      781 taccattcgt tcacagtgac atggaaattg atatgttagg tgatgcatat gaattcctaa
      841 tcgggcgctt tgcggcgaca gcgggtaaaa aagcaggcga gttctataca ccacaacaag
      901 tatctaagat actggcgaag attgtcacag acggtaaaga taaattacgt cacgtgtacg
      961 acccaacatg tggttccggt tcattgttgt tacgtgttgg taaagaaacg caagtgtatc
     1021 gttatttcgg acaagaacgt aacaatacta catacaactt agcacgcatg aatatgttat
     1081 tacatgatgt gcgttatgag aacttcgaga tccgtaatga tgacacattg gaaaatccag
     1141 cctttttagg caatacattt gatgcggtta ttgcgaaccc accatacagt gcgaaatgga
     1201 cagcagattc aaaatttgaa aatgatgaac gattcagtgg ttacggcaag cttgcgccaa
     1261 aatccaaagc agactttgcc tttattcaac acatggtaca ttacctagac gatgaaggta
     1321 ccatggcagt cgtactccca catggtgtat tattccgtgg tgccgcagaa ggtgtgattc
     1381 gtcgctattt aatagaagaa aagaactact tagaagccgt gattgggtta ccagccaata
     1441 ttttctatgg gacaagtatt ccaacatgta ttttagtatt taaaaaatgt cgccaacaag
     1501 acgacaacgt actatttatc gatgcatcca atgattttga aaaaggaaaa aatcaaaacc
     1561 atttaagcga tgcccaagtc gaacgtatta tagacacata taagcgtaaa gcaacgattg
     1621 ataaatatag ctacagcgcg acattacaag agatcgccga taacgattac aacttaaaca
     1681 taccgcgata tgttgataca ttcgaagaag aagcaccaat tgatttagat caagtccaac
     1741 aagatttgaa aaatatcgac aaagaaatcg cagaaattga acaagaaatc aatgcatacc
     1801 tgaaagaact tggggtgttg aaagatgagt aatacacaaa cgaaaaatgt gccagagttg
     1861 agattcccag gatttgaggg cgaatgggaa gagaagaagt tagggaa
//
LOCUS       DAMZKC010000277         1899 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.281, whole genome shotgun sequence.
ACCESSION   DAMZKC010000277 DAMZKC010000000
VERSION     DAMZKC010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.281"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            243..1652
                     /locus_tag="POB55_002680"
     CDS             243..1652
                     /locus_tag="POB55_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDI6501587.1"
                     /translation="MSIDMYLDRSRNQASSVGNLSQTMNSNYDALEKAITQFINDDVL
                     KGKAYTSAKQFFSTVLIPLSTSMKTLSDLTKQACDNFVSRYTSEVDSISLKESELEED
                     IRSLSQQITRYENLNNNLKKHASDNQQAISSNQQIIQTLGQQKHELEEKLRKLREFNQ
                     KSPEIFKEVEEFQKIVQQGLTQAQNFWNFSTNQFNIPSGKELDWAKASHEKYLKVAMG
                     KIEHKAEKETLNKADFAVIKAYAKEHPEDDIPKSILKYINDNKDSIKRDIGLDITSTL
                     LEQGGINASKFGVFINTAGGVKGPAGPNSFVEVKRTSGNVFIENGSKFAKGGKYLGKG
                     VAGVGFGIGMYDDLANDDKTFGEALSHNGMTLAAGSAGTAVGAGLATFVLGSNPVGWV
                     ILAGLAMSTVFALGTDLIYQNNIFGLKDKVDWVGHKIDNSIDVVKKTTEKSMDSVGNA
                     VSEAKNIISNHINPMKWAW"
     gene            1656..>1899
                     /locus_tag="POB55_002681"
     CDS             1656..>1899
                     /locus_tag="POB55_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001836194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HDI6501588.1"
                     /translation="MLFNVKVINKNPRYKVVQYNDEYLLIDLVSTWLVYFFPFINWFI
                     PKRYAKLSEKELENLNVDKQNKNNIFWPVTGSSFLFV"
BASE COUNT          719 a          234 c          371 g          575 t
ORIGIN      
        1 taaaattaaa atattgaaag ataattttta ttgattaatt gcgaatatac aaagacacca
       61 ttatatccat atgctaaaat aagaaaaaag gcgacatgtt atctcaattt acctgcatct
      121 agactccttt acataagaaa taatcatcgc cgagttttaa aattttacaa ctttgtagat
      181 gtaaaaagca tttgatttta aatagtttga aaatcatata tttttgaaaa aggagcatgc
      241 cgatgagtat tgacatgtat ttagacagat ctcgaaacca agcttcaagt gtggggaatt
      301 tgagtcaaac aatgaattca aattatgatg cgttggaaaa agcaattact caatttatca
      361 atgatgatgt gcttaaaggg aaagcgtata cgtcagctaa gcaatttttt agtacggtgt
      421 taattccatt atcaacaagt atgaaaacat tgagtgattt aacgaagcaa gcttgcgata
      481 attttgtgtc acgttatacg agtgaggttg atagcatatc tttaaaagaa tcagagcttg
      541 aagaagatat cagatcatta agtcaacaaa ttacgcgata tgaaaatttg aataacaatt
      601 tgaaaaagca tgcttccgat aatcagcaag ccatttcatc gaaccaacaa ataatacaaa
      661 cattaggtca acaaaaacat gaattagaag agaagctacg caaattgcgt gagtttaatc
      721 aaaaatcacc agaaatattt aaagaagttg aagaatttca aaaaattgtc caacaaggac
      781 ttacccaagc gcaaaatttt tggaactttt caacaaatca atttaatatt ccttcaggta
      841 aagaacttga ttgggccaaa gcaagtcatg aaaaatattt gaaagttgct atggggaaaa
      901 ttgaacataa agcagagaaa gaaactttaa ataaagcaga ctttgctgtt ataaaggcat
      961 atgccaaaga acatccagaa gacgatatcc cgaaaagtat attgaaatat ataaatgaca
     1021 ataaagacag tattaaaaga gatataggat tagatattac gtcaacactt ttagagcaag
     1081 gcggtataaa tgcaagtaaa ttcggtgtat ttatcaatac agcaggtgga gtgaaaggcc
     1141 cagcaggtcc aaattcattt gtggaagtca aacgtacatc aggtaatgtg tttatagaaa
     1201 atggtagtaa atttgcaaaa ggcggaaaat acctaggtaa aggtgttgct ggtgtaggat
     1261 ttggtatagg tatgtatgat gaccttgcaa atgatgataa aacatttgga gaggcgttgt
     1321 cgcataatgg tatgacgctt gcagctggat ctgcagggac agcagttgga gctggattag
     1381 cgacttttgt tttaggaagt aatccagtag gatgggtgat tttagcaggt ttggctatga
     1441 gtacagtatt tgcattagga acagatttaa tttatcaaaa taatattttt ggattaaaag
     1501 ataaagtaga ctgggtaggg cataaaatcg ataatagtat agatgttgtt aaaaaaacta
     1561 cagaaaaatc catggatagt gttgggaatg ctgttagtga agctaaaaat attattagca
     1621 atcatataaa tccaatgaaa tgggcgtggt gagttttgct ttttaatgtt aaggttatta
     1681 ataaaaatcc gagatataaa gtcgttcaat ataatgatga gtatttgtta attgatttag
     1741 taagtacttg gcttgtatac ttttttcctt ttattaattg gttcattccc aaaaggtatg
     1801 caaaacttag tgagaaggaa cttgaaaatt taaatgttga taaacaaaat aaaaataata
     1861 ttttctggcc agttactggt agttcgtttt tatttgtag
//
LOCUS       DAMZKC010000278         1864 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.282, whole genome shotgun sequence.
ACCESSION   DAMZKC010000278 DAMZKC010000000
VERSION     DAMZKC010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.282"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..239
                     /locus_tag="POB55_002682"
     CDS             <1..239
                     /locus_tag="POB55_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501589.1"
                     /translation="NVTCNYIKTKLEELDNTLGKWEPFLNDLKAVSPILDNHLDDIVK
                     NMKPLIVQMIFEPSHYDDMFISRKALTPVFSSVL"
     gene            complement(282..1487)
                     /locus_tag="POB55_002683"
     CDS             complement(282..1487)
                     /locus_tag="POB55_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDI6501590.1"
                     /translation="MWIEKFKNKNNETKYRYYEKYKDPYTDKWKRVSVVLNKNTKQSQ
                     KEAMFRLEEKIKEKLNNKSSSELKTLTFHALLDEWLEYHIKTSGSKLTTLNNIKIRIR
                     NIKRYSSENLLLNKLDTKYMQIFINKLSDIYSQNQVTRQLGDMKGAIKYAVKFYNYPN
                     EYLLTNVKIPKRRKTIEDIEKDESKMYNYLEMNQVLQIRDHILNDNKLHKRNRILIAS
                     ILEVQALTGMRIGELQALQEKDIDLLNKTINITGTIHRIKYEEGFGYKDTTKTISSKR
                     SISINSRTVEIFKKIILENKMLKRWNSSYVDRGFIFTTKKGNPLCNNQIAGVLKKTTK
                     ALNMNKKVTTHTFRHTHITLLVEMNVSLKAIMKRVGHVDEKTTIRIYTHVTEKMDREL
                     TQKLENIPS"
     gene            1613..>1864
                     /locus_tag="POB55_002684"
     CDS             1613..>1864
                     /locus_tag="POB55_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDI6501591.1"
                     /translation="MTQFLGALLLTGVLGYIPYKYLTMIGLVSEKNKVINTPVLLIFS
                     IETCLIWFYSFIIFNNVDLKNLNLIQLLTGLKANILFLFI"
BASE COUNT          546 a          258 c          280 g          780 t
ORIGIN      
        1 ctaatgtaac ttgtaattat attaaaacta aattagaaga attagataat actttaggca
       61 aatgggagcc ttttcttaat gatttaaaag cagtgtcacc aattttagat aaccatttag
      121 atgatattgt taagaacatg aagcctttga ttgtacaaat gatatttgaa ccatcacatt
      181 atgatgatat gtttatttca agaaaagctt taacgccagt gttctcaagc gttttataaa
      241 gcttgtaaaa aatataaggg caaaaaaagg gcggatttaa gctaacttgg aatgttttcg
      301 agtttttgag ttagttctct atccattttt tcagttacat gagtatatat gcgaatggtt
      361 gttttttcat ctacatgtcc tacccttttc ataattgctt ttaaagaaac attcatttct
      421 actaataaag ttatgtgtgt atgtctaaat gtgtgcgtgg taactttctt attcatattt
      481 aaagcttttg tagttttctt aagcacaccg gcgatttgat tattacataa aggattccct
      541 ttttttgttg tgaatatgaa ccctctgtca acatagctcg aattccatct tttcaacatt
      601 ttgttttcca gtattatctt tttaaaaatt tctacggttc tagaattgat gctgatactt
      661 ctttttgaac ttatagtctt tgtagtgtct ttgtatccga atccttcctc gtatttaatg
      721 cggtgaattg tacctgttat attgatagtt ttgtttaata aatctatatc tttttcctgc
      781 agtgcttgta gttctcctat gcgcatacca gttaaagcct gtacttctaa gatgctggca
      841 attaaaatgc gatttcgctt gtgtaactta ttatcattta gtatatgatc acgtatctgt
      901 aggacttggt tcatttctaa atagttgtac attttagatt catctttttc gatatcctct
      961 attgtttttc ttcttttagg aattttgaca ttagttaaca aatattcatt tggataattg
     1021 taaaatttaa ctgcatattt aatagctcct ttcatatctc cgagttgacg ggttacttga
     1081 ttttgagaat agatatctga taatttatta ataaatatct gcatatattt tgtatctagt
     1141 ttgtttaaaa gcaagttctc agagctgtat cgtttaatgt ttctaattct tatttttata
     1201 ttattaagag tagtcaactt tgaacctgat gtttttatat gatattcaag ccattcatct
     1261 aatagcgcgt gaaaagtcaa agtttttaat tcgcttgacg acttgttgtt cagtttttct
     1321 tttatttttt cttctaaacg aaacattgct tctttttgag attgttttgt attcttgttc
     1381 aacacaacac ttacgcgctt ccatttatct gtgtatggat ctttatactt ctcgtagtat
     1441 ctgtatttag tttcgttatt tttgttttta aatttttcaa tccacatgtt tatacctcct
     1501 gcaagaacgt atgttctata aaatattaaa aaataataag ggtagtcggg ctacccgtaa
     1561 tttagtacta ggtactaaat gtgttataat aaaataaaaa gtaggtgata agatgactca
     1621 atttctaggg gcgcttcttc ttacaggagt tttaggttac ataccatata aatatctaac
     1681 aatgataggt ttagttagtg aaaaaaacaa ggttatcaat actcctgtat tattgatttt
     1741 ttctattgaa acatgtttga tatggtttta tagttttata atttttaata atgttgattt
     1801 aaaaaatttg aatttaattc agttgcttac aggtctaaaa gcaaatattt tgtttctatt
     1861 tatt
//
LOCUS       DAMZKC010000279         1863 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.283, whole genome shotgun sequence.
ACCESSION   DAMZKC010000279 DAMZKC010000000
VERSION     DAMZKC010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.283"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..1335
                     /locus_tag="POB55_002685"
     CDS             <1..1335
                     /locus_tag="POB55_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HDI6501592.1"
                     /translation="KCTYWVTLIISQILLTTSVLWILNYTGNLDLFILRLVLLSCLFI
                     LTISVINLWTFLMLLNLNIANMIKGKQHFKTIRFINTVCKSILLVLIANVMIENTSVI
                     KDLNKIKETEKYWNVLDDYYTIEFAPYHETKQSLIDNMVRSEQLVKASEAENNAILFK
                     PKGDSVDNDNFSPDEGNVILVNNQFWSIYHKQFQPDIPIKNQKNNVEVIIPQKFHAMR
                     NEINQAYHSWFEFVQNKNNKESKLSIQFINKNDYRIFTFDARDSRHLSFIEAPIIVNV
                     QASDLSNDFYYAMISQGGYLFKNYDALVKNIEKYHLDGEISGITNYKDSVMEMYHENN
                     LKLTVLNFSQIIIAIILIIIILFDVKYYFEQHRKLLVIKKLFGYSTLRANYQYLLINN
                     IVVVFIGILTNVILHSQYIMMIFATIIVVQILLQICSLYYHGRRFNEVIKEF"
     gene            1338..1658
                     /locus_tag="POB55_002686"
     CDS             1338..1658
                     /locus_tag="POB55_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDI6501593.1"
                     /translation="MKYIIAILLGLIVSITIAFTIIHHPILDRFNPFLKTEYSYAKVP
                     KGTQQYVNITAYSERGEKLDYKLTFNGFSPSRTYVEIKHKGQYVISITYVEKEDTPKE
                     VRQE"
     gene            1655..>1863
                     /locus_tag="POB55_002687"
     CDS             1655..>1863
                     /locus_tag="POB55_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDI6501594.1"
                     /translation="MIELKDLTIQKGNTHILKKLNLKFQCGKSYALIGKSGCGKSTLL
                     NTIAGLEKTGKQYVYFNGQLEQFKS"
BASE COUNT          711 a          231 c          291 g          630 t
ORIGIN      
        1 aaatgcacgt attgggtaac gttaatcata agtcaaattc ttttaactac aagtgtactt
       61 tggatattaa attacacggg taatttagat ttattcatat taagattagt tctgctttca
      121 tgtcttttca ttttaacgat tagtgttatt aatttatgga ctttcttaat gttactaaat
      181 ttaaatattg ctaatatgat taaaggtaag cagcacttta aaacaattcg ttttattaat
      241 acagtttgta aaagtattct cttagtacta atagctaatg taatgataga aaatactagt
      301 gttattaaag atttgaataa aataaaagaa actgagaaat attggaatgt attagatgat
      361 tattacacga ttgaatttgc accttatcac gaaacaaaac aaagtttgat tgataatatg
      421 gtgcgatcag aacaattagt aaaggctagt gaagcagaaa ataatgcgat tttattcaaa
      481 ccaaagggtg actccgttga caatgacaac ttttcacctg atgaggggaa tgtaatatta
      541 gtaaataatc agttttggtc gatttatcac aagcagtttc aacctgatat tccaataaaa
      601 aatcaaaaaa ataatgtcga agtaattatt ccacaaaagt ttcatgcaat gcgtaatgaa
      661 atcaatcaag catatcattc atggtttgaa tttgtacaaa ataaaaataa taaagagagt
      721 aagttatcta tacagtttat caacaaaaat gattatcgaa tttttacatt tgatgcacga
      781 gatagtcgcc atttgtcatt tatagaggcg ccaatcattg tgaatgttca ggcatcagat
      841 ttatcgaatg atttttatta tgccatgatc agtcaaggcg ggtatttatt caaaaattat
      901 gacgcgctag taaaaaatat tgaaaagtat catcttgatg gggaaatcag tggaataacc
      961 aattataaag atagcgtgat ggaaatgtat catgaaaaca atttgaaatt aacagtactc
     1021 aacttttcac aaatcattat cgcaatcatt ttaataatta ttattttatt tgatgtgaaa
     1081 tattattttg aacaacatcg aaaattactc gtaatcaaaa agctatttgg ttattcaaca
     1141 ttaagagcca attaccaata cttattaata aataatatag ttgttgtttt tattggaata
     1201 ttgacgaatg taattttaca ttctcagtat ataatgatga tatttgcaac gattattgtt
     1261 gttcaaatat tattgcagat ttgcagccta tactatcatg gccgacgttt taatgaagtt
     1321 atcaaggagt tttaactatg aaatatatca ttgcaatatt attaggatta atcgtatcca
     1381 ttactattgc ttttacaatc atacatcatc ctatacttga tcgttttaat cctttcttaa
     1441 aaacggagta tagttatgcc aaagtgccaa aaggtacgca acaatatgtt aatattacgg
     1501 cttatagtga aagaggggaa aagcttgatt ataaattaac atttaatgga ttttcaccta
     1561 gtagaacgta tgtcgaaata aagcataaag ggcaatatgt catatcgatc acatatgttg
     1621 aaaaagagga tacaccaaaa gaggtaagac aagaatgata gaattaaaag atttaaccat
     1681 acaaaaaggt aatacacata ttttgaagaa acttaatttg aaatttcaat gtggaaaatc
     1741 atatgcactt attggtaaaa gtggatgcgg taaatctaca ttattaaata ctattgctgg
     1801 acttgaaaaa acggggaaac aatatgtcta ttttaatggt caattagaac aatttaaatc
     1861 taa
//
LOCUS       DAMZKC010000280         1842 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.284, whole genome shotgun sequence.
ACCESSION   DAMZKC010000280 DAMZKC010000000
VERSION     DAMZKC010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.284"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            142..642
                     /locus_tag="POB55_002688"
     CDS             142..642
                     /locus_tag="POB55_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HDI6501595.1"
                     /translation="MTFEEKLNEMYNEIANKISSMIPVEWAKVYAMAYIDEESGEVFF
                     NYTEPRSDELFYYTSVIKKYNISRSGFMNSVYELHDQFEKLRELFIEEGHEPWTSCEF
                     DFTKEGKLKVSFDYIDWLNTEFDQLGRENYYMYKKFGVIPEMEYEMEEIKEIEQYIKE
                     QDEAEQ"
     gene            653..1153
                     /locus_tag="POB55_002689"
     CDS             653..1153
                     /locus_tag="POB55_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HDI6501596.1"
                     /translation="MTFEEKQSEMYNKIANEISGMIPVEWEKVYTIAYVDNQGGEVIF
                     NYTKPGSDELNYYTDISRDYNISEEIFDDLWMNLYYLFMNLRDLFKKEGLEPWTSCEF
                     DFTSEGKLKVSFDYIDWINTEFDQLGRENYYMYKKFGVLPEMEYEINKVKEIEQYIKE
                     QDEAEL"
     gene            1164..1652
                     /locus_tag="POB55_002690"
     CDS             1164..1652
                     /locus_tag="POB55_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HDI6501597.1"
                     /translation="MTFEEKLSQMYNEIANEISGMIPVEWEKVYTIAYVDDEGGEVVF
                     NYTKPGSDELNYYTYIPREYNVSEKVFYDLWTDLYRLFKKLRNAFKEEDLEPWTSCEF
                     DFTREGNLKVSFDYIDWIKLGFGPSGKENYYMYKKFGVLPETEYEMEEIREVEKYVKD
                     QE"
BASE COUNT          724 a          169 c          375 g          574 t
ORIGIN      
        1 aacaatagtt tcagttattt tagtttcatt agtttattta ggtttaaaat gagtaatatt
       61 attttgaaga agcattaagc cacgtagagg tgttgtttaa tgctttttat ttaacggtaa
      121 tgaaaatata ggggagataa catgactttc gaagaaaaac taaatgaaat gtacaacgag
      181 attgcgaata agattagcag catgataccg gtagagtggg caaaggtata tgcaatggca
      241 tatatcgatg aagaatctgg agaagtgttt ttcaattata ctgaaccaag aagtgatgaa
      301 ttattttact atacaagcgt gataaaaaag tataatattt cgagatcagg atttatgaat
      361 tcagtatatg agttgcatga tcaatttgaa aaactaagag aattgtttat agaagaagga
      421 catgaaccat ggacatcatg tgagtttgat tttacaaaag aaggtaaatt gaaagtatct
      481 tttgattata tagattggtt aaatacagaa tttgatcaat tgggtcgtga aaattattat
      541 atgtacaaaa aatttggggt tataccagaa atggaatatg aaatggaaga aattaaagaa
      601 atcgagcaat atattaaaga gcaagatgaa gctgaacaat agaggcgata acatgacttt
      661 cgaagaaaaa caaagtgaaa tgtacaataa aattgcaaat gagattagtg gaatgatacc
      721 ggttgagtgg gaaaaggtat atacaattgc ttatgtagat aatcaaggtg gagaggtcat
      781 ttttaattat actaaaccag gtagcgatga attgaattat tacacggata tatctagaga
      841 ttataatatt tcagaagaaa tatttgatga tttatggatg aatctttatt acttgtttat
      901 gaatttgagg gatttattta aaaaagaagg tcttgaacca tggacatcat gtgaatttga
      961 ttttacaagc gaaggtaaat taaaagtttc atttgattat attgattgga taaatacaga
     1021 gtttgatcaa ttaggccgtg aaaactatta tatgtataaa aagtttggtg ttttaccaga
     1081 aatggaatac gaaattaata aagttaaaga aatcgagcaa tatattaaag agcaagatga
     1141 agctgaacta taggggcgat aacatgactt tcgaagaaaa attaagtcaa atgtacaacg
     1201 agattgcgaa tgagattagt gggatgatac cagtagagtg ggaaaaagta tatacaattg
     1261 cttatgtaga tgatgaaggt ggagaagtcg tttttaatta tactaaacca ggtagtgatg
     1321 aattgaatta ttacacatat atccctagag agtataatgt ctctgaaaaa gtattttatg
     1381 atttgtggac ggatttatat agattgttta agaagttaag aaacgcattt aaagaagaag
     1441 atttagaacc atggacatca tgtgaatttg attttacaag agagggtaat ttgaaagtat
     1501 cgtttgatta tatagattgg attaaattag gttttggtcc atcaggaaag gaaaactact
     1561 atatgtataa aaagtttggt gttttaccag aaacggaata tgaaatggaa gaaattcgag
     1621 aagtagagaa gtatgttaaa gaccaagagt agcatatatg ttatagaagg ctgtgcaaaa
     1681 tcaccctagt tttacatttg actcaaagaa gaaggtaaac gataagatta tttgcaactt
     1741 aaaaagtcta ttagcttctc ggtatccata catcatatgt ataaatgagt ttaactaatt
     1801 aataaatcac gatataaatt taaaattgcc attaattgaa aa
//
LOCUS       DAMZKC010000281         1838 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.285, whole genome shotgun sequence.
ACCESSION   DAMZKC010000281 DAMZKC010000000
VERSION     DAMZKC010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.285"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(39..1676)
                     /locus_tag="POB55_002691"
     CDS             complement(39..1676)
                     /locus_tag="POB55_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HDI6501598.1"
                     /translation="MDKELWIERANDSLVKHFYEQQSDIEQREGFESKLTFGTAGIRG
                     KFGLGEGRLNKFTIEKLALGLARYLNAQTNSPTIVIHYDIRHLSTEFAQIIANVLANH
                     QITVYLPDTYKTTPELSFAVRNLNTTAGIMITASHNPKDYNGIKVYGSDGAQLSTDAS
                     ELASRYIEEVGDPLQIDIPISKQNTSYIKPFPKSVTDDYMKHIQNMIGYIPKSDLQVV
                     FTSLHGTSVPIVPELLQSLNFNQFNLVEAQCKPDPNFSSVQSANPEDHRAFDQAVELA
                     NKSHADLLISTDPDADRLGIAERDAHGHITYFNGNQIGALLLNYRIQQTSQLRHRLMI
                     QSIVSSELTKSLARYNNVEYKEVLTGFKFIAQEIRQLDDHQNMIFAFEESYGFLSEPF
                     VRDKDAVQIVPLIIKYASELKLYGKTLKDELEQIYQTVGRHEDTLFSHTLDGLEGKKK
                     IESIMTHFRSNPPQEIQGLKVKAIEDYLTSEVYHLDKDTTSQINSPKSNVIRVLFDEG
                     FIALRPSGTEPKIKLYVSLKCRNFDDVAQKINAMIFS"
BASE COUNT          606 a          323 c          292 g          617 t
ORIGIN      
        1 aaaatgaaat gtaatatttc gatgtataaa atttttaatt agctaaaaat catcgcatta
       61 attttttgag ctacatcatc aaaattccga cattttaacg acacatataa tttaatttta
      121 ggttcagtac cagaaggacg taaagcgata aatccttcgt caaataagac acgaataaca
      181 tttgatttag gagaattaat ctgtgacgtt gtatctttat ccaaatgata aacctcgcta
      241 gttaaataat cttcaattgc tttcactttc agtccttgaa tctcttgcgg tggatttgaa
      301 cggaaatgtg tcataattga ttcaattttt ttcttacctt ctaatccatc tagcgtatgc
      361 gagaataatg tatcctcatg tctaccaaca gtttgataaa tctgttctaa ttcatctttc
      421 aatgttttgc catataattt taactcagaa gcgtatttta taatgagtgg cacaatttgt
      481 acggcatctt tatcacgtac aaaaggctct gatagaaaac cgtaactctc ttcaaaggcg
      541 aaaatcatat tttgatgatc gtccagttgt cttatttcct gagcaataaa tttaaagccc
      601 gtaagcacct ctttgtattc aacattatta tagcgtgcta atgactttgt taactcacta
      661 cttacaatag attgaatcat taagcgatga cgtaattgag acgtttgttg aatacgataa
      721 ttaagtaaca acgcaccaat ttggttgcca ttaaaatatg tgatatgacc atgtgcatca
      781 cgttcagcaa tgccaagacg atccgcatca gggtcagttg atattaataa gtctgcatga
      841 cttttatttg caagttcgac tgcttgatca aaagcgcgat gatcttcagg attagcactt
      901 tgaactgagc tgaaattcgg atcaggttta cattgtgctt ctacaagatt aaattgattg
      961 aaattcaaag attgtaataa ctcaggtaca atcggaacac ttgtaccatg taaactagtg
     1021 aacacaactt gtaaatcaga tttaggaata tagccaatca tattttgaat gtgtttcata
     1081 tagtcatcag ttacagattt cggaaaaggc ttgatataag atgtattttg tttagaaata
     1141 ggtatatcaa tctgtaatgg atcgccaact tcttcgatat aacgacttgc aagctcagat
     1201 gcatcagtcg ataattgcgc accatcagaa ccatatactt tgatgccgtt atagtctttc
     1261 ggattatgac ttgctgtaat cataatgcca gcagtagtat taagattgcg caccgcgaac
     1321 gataattccg gtgtcgtttt atatgtatca ggtaaataaa ctgttatttg atggtttgct
     1381 aatacattag caattatttg ggcgaattca gttgaaagat gtctaatatc ataatgaatg
     1441 actattgttg gactgtttgt ttgggcattt aaataacgcg ctaaacctaa tgccaatttt
     1501 tcaatagtaa acttattaag tcgaccttca ccaagaccga attttccgcg tatacccgca
     1561 gtaccaaatg ttaatttact ttcaaaacct tctcgctgtt caatgtcaga ttgctgctca
     1621 taaaaatgtt taactaaact atcattagct cgttctatcc ataattcttt atccattgtt
     1681 gctaaacatc ctttcaaaat ctcagttaga cttaataaaa catgaaaact aaagccctta
     1741 catttatgta atgaattata aagaaatacg ccccaaaagt aaaaaaacac agccccaaga
     1801 caatactttt cacaagtatt atataataga tgtgtatg
//
LOCUS       DAMZKC010000282         1747 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.286, whole genome shotgun sequence.
ACCESSION   DAMZKC010000282 DAMZKC010000000
VERSION     DAMZKC010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.286"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            4..903
                     /locus_tag="POB55_002692"
     CDS             4..903
                     /locus_tag="POB55_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000614797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6501599.1"
                     /translation="MIQISNINKSFNKRCVLKNISFDIEQGKCIALIGKNGAGKSTLI
                     DILIGNVNANSGEIFDKDKLLQSENRSIMFQKTMFPDQLKVIEIINLYQSFYENPLPL
                     EEIIELTKFDSSQLNQFVNKLSGGQQRLLDFVLSLIGQPQLILLDEPTSTMDIEIREY
                     FWSIIENLKEDNRTILYTSHYIEEVERMSDKIILIENGEIILNDSTSHIRTNQQSQIT
                     LSDEYIRKLKLDKDDLVIQKNHNGTIKIITSNVNDTILYLQQLHINLDDIEIQKVSIV
                     DSYFNNKKQRGSNYDTKLLENRI"
     gene            872..1603
                     /locus_tag="POB55_002693"
     CDS             872..1603
                     /locus_tag="POB55_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6501600.1"
                     /translation="MILSYLKIEFKVIMRKKTTLILSILFPVIFYILFTSILELPEDV
                     KPKFYKEYMYSMTVYSLLSFSLLTFPLDIINEKQNEWRQRLMVTPFTFTSYYISKVVK
                     TMLQFAIAILVIFMVGHFYKGVAMSAVQWLESGIFLWLGASLLITFGILFSLLNDIQK
                     TSALANIVTIGLAVLGGLWFPINTFPNWLQHVAHVLPSYHLRKLGVDIASNHHINLIS
                     FAIILLYALGSILAVYCISHFKRAE"
     gene            1607..>1747
                     /locus_tag="POB55_002694"
     CDS             1607..>1747
                     /locus_tag="POB55_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDI6501601.1"
                     /translation="MKFLKDTSIAEISSILYLIFPIAGIFFNEVYGPKWLYIISVIVF
                     SLS"
BASE COUNT          635 a          216 c          264 g          632 t
ORIGIN      
        1 aaattgattc aaatatctaa tatcaacaag tcatttaata aaagatgtgt tctaaaaaat
       61 atttcgttcg atattgaaca aggtaaatgt atcgctttaa ttggaaaaaa tggtgctgga
      121 aagtcaacgt taattgatat attaattggt aatgttaatg ctaattctgg tgagatattt
      181 gataaagaca agttattaca aagtgaaaat cgcagtataa tgttccaaaa aacgatgttt
      241 ccagatcaat taaaagttat tgagattatc aacttatatc aatcatttta cgaaaatcca
      301 ttaccattgg aagaaataat agaactgacg aaatttgatt ctagtcaact gaaccaattt
      361 gtaaataaac tttctggtgg tcaacaacga ttactcgatt ttgtattatc tttaatcgga
      421 caaccacaat tgatcttatt agatgaacca acatcgacta tggatataga aattagagaa
      481 tatttttggt caattattga aaatttaaaa gaagataatc gaacgatact ctatacatcg
      541 cactatattg aagaagtcga acgtatgtca gacaaaatta ttctcattga aaatggagaa
      601 ataatactta atgattcaac gtcacatatt agaaccaatc agcaatctca gattacgtta
      661 tccgatgaat atataagaaa gttaaaacta gataaagatg atttagttat tcaaaaaaat
      721 cataatggca ctatcaaaat tattacttca aatgtaaatg atacgatttt atatcttcaa
      781 caacttcata ttaatttgga tgatattgaa atacaaaaag tctcaattgt tgattcatac
      841 ttcaacaata aaaagcaaag gggatctaat tatgatacta agttacttga aaatcgaatt
      901 taaagttata atgcgtaaaa aaacaacatt aatattatct attttatttc ctgttatatt
      961 ctatatatta tttacttcga tattggaatt gccggaagat gttaaaccta aattttataa
     1021 agagtatatg tatagtatga cggtttatag tttgttaagt tttagtttac taacttttcc
     1081 attagatatt attaatgaaa aacaaaatga atggcgccaa agattaatgg taacaccatt
     1141 tacttttact agttattata tttcaaaagt agtgaaaact atgctgcaat ttgcaatagc
     1201 gatattagtt atttttatgg ttggacattt ttataaaggt gttgcaatga gtgcagttca
     1261 atggttagag tcaggaatat ttttatggtt aggtgcgtct ctattaataa cttttggcat
     1321 attattttct ttgttaaatg atattcaaaa aacaagtgct ttagctaata tcgtaacaat
     1381 tggtttagca gtattaggtg gattgtggtt tccgataaac acatttccaa attggcttca
     1441 acatgttgct catgttttac cgagctatca tttgcgtaaa ctaggtgtag atattgcttc
     1501 aaatcatcat atcaatttaa tatcatttgc tataatactc ttgtatgctt tagggagtat
     1561 attagcagta tattgtatta gtcattttaa aagggcggaa taaaatatga aatttttaaa
     1621 agatacttca attgctgaaa tatcgtctat actttatctg atttttccta ttgccggtat
     1681 attttttaat gaagtatatg gtcccaaatg gttgtatatt atatcagtca ttgtcttttc
     1741 gttgtcg
//
LOCUS       DAMZKC010000283         1747 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.287, whole genome shotgun sequence.
ACCESSION   DAMZKC010000283 DAMZKC010000000
VERSION     DAMZKC010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.287"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            52..696
                     /locus_tag="POB55_002695"
     CDS             52..696
                     /locus_tag="POB55_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HDI6501602.1"
                     /translation="MKVKYIDKRHWRRLIDREYTEVKVNNNRFKGIIGLVTMKKVRDP
                     LEVTVVGQNIIVADDNYKWLQILPEKKRYSITVMFDNKGNPLEYYFDINIKNITQKGN
                     ARTVDLCLDVLALPSGEYELVDEDDLMFALESEQITKKQFHEAYMIAHQIMAELENDF
                     KGFQKKIMYCFNKINAKAQKNHQKPQNKTNIEKSKQIKPKQYNQTKNHQQQKKN"
     gene            812..1678
                     /locus_tag="POB55_002696"
     CDS             812..1678
                     /locus_tag="POB55_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI6501603.1"
                     /translation="MKIEDYRLLITLDETKTLRKAAEILYISQPAVTQRLKAIENAFG
                     VDIFIRTKKQLITTTEGTMIIEHARDMLKRERLFFDKMQAHIGEVNGTISIGCSSLIG
                     QTLLPEVLSLYNAQFPNVEIQVQVGSTEQIKANHRDYHVMITRGNKVMNLANTHLFND
                     DHYFIFPKNRRDDVTKLPFIEFQADPIYINQIKQWYNDNLEQDYHATITVDQVATCKE
                     MLISGVGVTILPEIMMKNISKEQFEFEKVEIDNEPLIRSTFMSYDPSMLQLPQVDSFV
                     NLMASFVEQPKA"
BASE COUNT          679 a          222 c          319 g          527 t
ORIGIN      
        1 catgacatca tttatgtagt tttatataat aatgacaagg agttggtgac tgtgaaagta
       61 aagtatatag ataaacgtca ctggcgtcgc ctaattgata gggaatacac agaggtaaaa
      121 gttaataata ataggtttaa gggtattata ggcttagtca cgatgaaaaa ggttcgtgat
      181 cctttagagg tgacggtagt tggacaaaat atcattgtcg cagatgacaa ttataaatgg
      241 ttgcaaatac tacctgaaaa gaaacgttat agtataactg taatgtttga taataaaggc
      301 aatccattag aatattattt tgatataaat atcaaaaata taacgcaaaa aggtaatgcg
      361 cgtacagtag atttatgttt agatgtttta gcgttaccaa gtggtgaata tgagttggta
      421 gatgaagatg acttaatgtt tgcattagaa agtgagcaaa ttacaaaaaa gcaatttcat
      481 gaagcatata tgattgcaca tcaaattatg gcagagttag aaaatgattt taaaggattc
      541 caaaagaaaa tcatgtactg ctttaataaa attaatgcaa aggctcaaaa aaatcatcaa
      601 aagccacaaa ataaaactaa tattgaaaaa agcaaacaaa taaagcctaa gcaatataat
      661 caaactaaaa atcaccaaca acaaaagaaa aactaagtaa ttcaagctgc agccatgcca
      721 ataaaattgg tgaaattatt cgttagtaat gaactcttga atgctcgtag tcatgaaagt
      781 tcaataattg aataatttat ggggggaaat tatgaagatt gaagactatc gtttactaat
      841 aacattagac gaaacgaaaa cgttacgtaa agcggctgaa attttatata tatctcaacc
      901 tgctgttaca caaagactaa aagctattga aaatgctttt ggagtagata tttttatcag
      961 aacaaaaaaa caattgatta caacaactga aggaacaatg attattgagc atgcccgtga
     1021 catgttgaaa agagagcgat tattttttga caaaatgcag gcacatattg gtgaagtgaa
     1081 tggaacaata tcaatcgggt gttcttcttt gattggacaa accttacttc ctgaagtttt
     1141 gagcctatat aatgcccaat ttcctaatgt tgaaatacaa gtgcaagttg gttcaactga
     1201 acaaattaaa gcaaatcata gagattatca tgttatgata actcgtggaa ataaagtaat
     1261 gaatttagct aacacacatt tatttaatga tgatcattat tttatttttc caaaaaatag
     1321 acgagatgat gttacaaagt taccatttat agagtttcaa gctgatccga tttatataaa
     1381 tcaaataaaa caatggtata acgataattt agaacaagat taccatgcaa ctattacagt
     1441 ggatcaagta gcaacttgca aagaaatgtt gattagtggt gtaggtgtta caattttgcc
     1501 ggaaattatg atgaaaaata tcagcaaaga acaatttgag tttgaaaaag tagaaattga
     1561 taatgaaccg ctgattcgtt cgacatttat gagttatgat ccgagcatgt tgcaattgcc
     1621 acaagttgat tcttttgtaa atctcatggc gagctttgtt gaacaaccaa aggcgtagtt
     1681 ttagactaat ttaaggtttg tatttagttt taaactattc ggttaaattg aacgtagttg
     1741 gttgcta
//
LOCUS       DAMZKC010000284         1733 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.288, whole genome shotgun sequence.
ACCESSION   DAMZKC010000284 DAMZKC010000000
VERSION     DAMZKC010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.288"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            111..1202
                     /locus_tag="POB55_002697"
     CDS             111..1202
                     /locus_tag="POB55_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDI6501604.1"
                     /translation="MIDFFKDNKRIERFSFIDNKVHMKETFNVDNKVCYQVFYDEKGY
                     PYISRNINANNGAVGKTYVLVNKKEFKNNLALCVYYLEKLIKDSKDSIMICDGPGSFP
                     KMFNTNHKNAQKYGVIHVNHHENFDDTGAFKKSEKYIIENANKINGVIVLTEAQRLDI
                     LNQFDVENIFTISNFVKIHNAPKHFQTEKIVGHISRMVPTKRIDLLIEVAELVVKKDN
                     SVKFHIYGEGSVKDKIAKMIEDKNLERNVFLKGYTTTPQKCLEDFKLVVSTSQYEGQG
                     LSMIEAMISKRPVVAFDIKYGPSDFIEDNKNGYLIENHNIKDMADKILQLVNNDVLAA
                     EFGSKARENIIEKYSTESILEKWLNLFNS"
     gene            complement(1291..1647)
                     /locus_tag="POB55_002698"
     CDS             complement(1291..1647)
                     /locus_tag="POB55_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDI6501605.1"
                     /translation="MKLGMLLIRWMLATSFLVHGTLKFVDLDGTIHFLGSLGLPPTIA
                     VLMSIGEVVGGLALIIGFLTPYVNVFYICVMLGSIFTLKLTRGYVSGYEFEIIHIVMN
                     LACISSYSWKKWLQFY"
BASE COUNT          706 a          210 c          279 g          538 t
ORIGIN      
        1 aaagactaaa aaatacgatt gaaattgaag agagtaaagg tatttcaaga tattttgata
       61 taacgactgg tacatatatt gcctacatta gaaaaagtaa atctgaaaaa gtgattgatt
      121 tctttaaaga taataaacga attgaacggt ttagttttat agataataaa gtgcatatga
      181 aggaaacatt taatgtagat aataaagttt gttatcaagt attttatgat gaaaagggat
      241 acccatatat ttcaaggaat attaatgcta ataatggtgc tgtaggtaaa acttatgtgt
      301 tagttaataa aaaagaattt aaaaacaatt tagcactgtg tgtttactat ttagaaaaac
      361 taataaaaga ttctaaagat agtattatga tttgtgatgg accagggagt tttccaaaaa
      421 tgtttaatac aaatcataaa aatgctcaga aatatggcgt tattcatgtt aatcatcatg
      481 aaaatttcga tgatacgggt gcatttaaaa aaagtgagaa atatattatt gagaatgcga
      541 ataaaattaa cggtgtaatt gtattaacag aggcacaaag attagatatt cttaatcaat
      601 ttgatgtaga aaatattttc actattagca attttgttaa gatacataat gctccaaaac
      661 attttcaaac tgaaaaaatc gtaggtcata tttctagaat ggtaccaacg aagcgaattg
      721 atttgcttat tgaagtggct gagttagtcg taaaaaaaga taattctgtt aaatttcata
      781 tatatggaga aggatctgtc aaagataaaa tagctaaaat gattgaagat aaaaatttag
      841 aaagaaatgt ttttcttaaa ggatatacaa caactccaca aaaatgcttg gaagatttta
      901 aattagtcgt ttctacatct caatatgaag gtcaagggtt aagtatgata gaagcaatga
      961 tttctaaaag gcctgttgtt gcctttgaca tcaaatacgg accaagtgat tttatagaag
     1021 ataataaaaa tggttattta atagaaaacc ataatattaa ggacatggct gataaaatac
     1081 ttcagcttgt taataatgat gtattagcag cggagtttgg ttcgaaagcg agagaaaaca
     1141 ttatagaaaa atattcaacg gaatcaatat tagaaaaatg gttaaatctt ttcaatagct
     1201 aaatgaaaaa atcctaggta ttcatgagta cctaggattt ttacgcatga tatatacaaa
     1261 aaaagagagg atagtcctct caaataatag ttaataaaat tgtagccatt tcttccagct
     1321 gtaactacta atacaagcta agttcattac gatatgtatg atttcaaatt cataaccact
     1381 tacatatcct cttgtaagtt taagtgtgaa gattgagcct aacataacac aaatataaaa
     1441 aacattaaca taaggcgtta aaaagccgat aataagtgct aaacctccaa caacttcacc
     1501 aattgacatc aatactgcga tggtaggtgg taaacctaaa gagcctaaga agtgaattgt
     1561 tccatctaaa tcaacaaatt tcaaagtacc atgcactaag aatgatgttg ctaacatcca
     1621 tctaattaaa agcataccta atttcatcat gtcacctcca acatacgtat ttttaaaaca
     1681 tagtccaaat tatatttgta aagatgtgga aagtaaagat tatttataaa aat
//
LOCUS       DAMZKC010000285         1721 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.289, whole genome shotgun sequence.
ACCESSION   DAMZKC010000285 DAMZKC010000000
VERSION     DAMZKC010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.289"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            347..745
                     /locus_tag="POB55_002699"
     CDS             347..745
                     /locus_tag="POB55_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl peptide receptor-like 1 inhibitory
                     protein"
                     /protein_id="HDI6501606.1"
                     /translation="MKKNITKVIIASTVIATGLLTQTNDAKAFFSYEWKGLEIAKNLA
                     DQAKKDDERADKLIKEADEKNEHYKGKTVEDLYVIAKKMGKGNTIAVVKIKDGGKNGY
                     YTFDITRPLEEHRKNIPVVKNGEIDSITWY"
     gene            696..797
                     /locus_tag="POB55_002700"
     CDS             696..797
                     /locus_tag="POB55_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501607.1"
                     /translation="MFQWLKTAKSIRLLGIKKHTELNSCTPNVRKQC"
     gene            complement(1115..1432)
                     /locus_tag="POB55_002701"
     CDS             complement(1115..1432)
                     /locus_tag="POB55_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding protein"
                     /protein_id="HDI6501608.1"
                     /translation="MKKNFIGKSILSIAAISLTVSTFAGESHAQTKVEKYNEYQTNFK
                     KQVNKKVVDAQKAVNLFKRTRTVATHRKAQRAVNLIHFQHSYEKKKLQRQIDLVLKYN
                     TLK"
BASE COUNT          627 a          260 c          215 g          619 t
ORIGIN      
        1 attaattatc aaaaaagcta tatgtataac aatttggagt gtttgtctac ccaacattgc
       61 aaacaaaatg caaagaaata tatatcgttt taacaataaa aattatccaa tcatagttga
      121 gtgacgttta ctactattat tatactatca acaaattcaa taatacctca aataaaaata
      181 ataccatcaa attcatcatt attctttttc gattgcatta aaccttatta atttgcactt
      241 aattttaatt aatgaagtgt taactggtaa atgcatgaaa ccgtttgtaa aatagcagtg
      301 tactcgcatt agtaagtaca aatttatttt acagggagaa cttattatga aaaaaaatat
      361 cacaaaagta attattgcat ctacagtcat cgctacaggt cttctaactc aaactaatga
      421 tgctaaggcg ttctttagtt atgaatggaa aggtttagaa atcgctaaaa atttagcaga
      481 ccaagctaaa aaggacgatg aacgtgctga taaattaata aaagaagccg atgaaaaaaa
      541 cgaacactat aaaggtaaaa ctgttgaaga cttatatgta atagctaaaa agatgggtaa
      601 aggcaataca atagctgtag taaaaatcaa agacggtggt aaaaatggtt attatacctt
      661 cgatatcact cgcccattag aagaacatag aaaaaatatt ccagtggtta aaaacggcga
      721 aatcgattcg attacttggt attaaaaaac atactgaatt aaatagttgt acgccaaacg
      781 ttagaaaaca atgctaacgt tttggcgtgc tttttttatt ttaagtaact tctaatttat
      841 ttagcatctt tacgactgtt taataaagca cgtatgatta acacggttgc cactatatcc
      901 gttacaattt ttatgattgt taatacattc gatttccttt tcacaatagc cacctcgctt
      961 gttcaaaaca taaacaacta ttgcattcac ttttaagtaa ttatttatat tatttatccc
     1021 aataagctca ccattcaaat aaacacaata ttataaaata attatacctt tggatatagc
     1081 aaaaagccac actctatagc atggcttcta tcaattattt taaagtatta tattttaaaa
     1141 ctagatcgat ttgtctttgt aattttttct tttcatagct gtgttggaaa tgaattaagt
     1201 taacagctct ttgtgcttta cggtgtgttg caacagttct tgtacgtttg aacaagttta
     1261 cagctttttg tgcgtccaca acttttttat ttacttgttt tttaaagttt gtttgatact
     1321 cgttatattt ttcaacctta gtttgtgcat gagattcacc ggcaaacgtt gataccgtta
     1381 aactaatagc agctatgctt aaaattgatt tcccaataaa attctttttc ataattaata
     1441 acacctatcc tcaaatattt attttgtata tatttataat aatagacaga tacaaaaaac
     1501 tacattaact atttattaac taaacattaa tatttgtgtc actacatttc tgaatttcaa
     1561 gaattttgct actccatatt ttaaccatca aattattgat ataatttatg actttagaaa
     1621 tccttttaaa tatgtatacg attcatgatt ttaactaaaa tttcaaattt cctgacttct
     1681 tggaatctta attttatact ttttaataat acacttttaa c
//
LOCUS       DAMZKC010000286         1719 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.290, whole genome shotgun sequence.
ACCESSION   DAMZKC010000286 DAMZKC010000000
VERSION     DAMZKC010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.290"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(124..1605)
                     /gene="cls"
                     /locus_tag="POB55_002702"
     CDS             complement(124..1605)
                     /gene="cls"
                     /locus_tag="POB55_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDI6501609.1"
                     /translation="MRYTFSNDLGTLFTIILAIGFIINLVLAFIIIFLERNRRTASST
                     WAWLFVLFVLPLIGFILYLFFGRTVSARKLNKNNGKVLTDFDGLLKQQIESFDKGNYG
                     TDNKQVQKHHDLVRMLLMDQDGFLTENNKVDHFIDGNDLYDQVLQDIKNAKEYIHLEY
                     YTFALDGLGQRILKALEEKLKQGLEVKILYDDVGSKKVKMANFDHFKSLGGEVEAFFA
                     SKLPLLNFRMNNRNHRKIIVIDGQLGYVGGFNIGDEYLGLGKLGYWRDTHLRIQGDAV
                     DALQLRFILDWNSQAHRPQFEYDVKYFPKKNGPLGNSPIQIAASGLASDWHQIEYGYT
                     KMIMSAKKSVYLQSPYFIPDNSYINAIKIAAKSGVDVHLMIPCKPDHPLVYWATYSNA
                     SDLLSSGVKIYTYENGFIHSKMCLIDDEMVSVGTANMDFRSFELNFEVNAFVYDEKLA
                     KDLRVAYEHDITKSKQLTKESYANRPLSVKFKESLAKLVSPIL"
BASE COUNT          589 a          320 c          220 g          590 t
ORIGIN      
        1 aacactttgt catagatttg aaatgacaaa tgtcattatt aggttcacat aatatattta
       61 tcgttatttt aattttgggc aaaataaaaa gagcctctat aatcgagact ccttacaaat
      121 aaattataaa attggcgaaa ctaatttagc taacgattct ttaaacttaa cagacagcgg
      181 tctattggca tatgattctt tggttagttg ttttgatttt gtaatatcat gttcataagc
      241 cacccttaaa tctttagcaa gcttttcatc atatacaaaa gcattgactt cgaaattcaa
      301 ttcaaaactt ctaaagtcca tattagcagt tccgactgaa accatttcat catctattaa
      361 acacatttta gaatgtataa atccattttc atacgtgtaa atttttacgc cactagataa
      421 taaatctgaa gcatttgaat atgtcgccca ataaactaaa ggatgatctg gcttacatgg
      481 aatcattaga tgaacatcta cccctgattt agctgcaatt ttaatggcat ttatatatga
      541 gttatccgga atgaaataag gcgattgcaa atataccgac ttcttagcgc tcataatcat
      601 ttttgtataa ccatattcaa tttgatgcca gtcactagcc agaccacttg cagctatttg
      661 aattggtgaa ttgcccaatg gtccgttctt tttagggaaa tacttaacat catattcaaa
      721 ctgtggacgg tgcgcttgcg aattccagtc taaaataaat cgcaactgca gtgcatcaac
      781 cgcatcccct tgtatacgta aatgcgtatc tctccaatac cctagtttac ctaatcctag
      841 atattcatca ccaatgttaa atcctccgac ataacctagt tgaccatcga ttacgatgat
      901 ttttctatga tttctgttat tcattctgaa attcaataac ggtaatttag aagcaaaaaa
      961 tgcttcaact tctccaccta acgatttaaa atgatcaaaa ttagccatct taactttctt
     1021 tgaaccaacg tcatcatata aaattttcac ttctagacct tgtttcaatt tctcttctaa
     1081 cgcttttaaa attctttggc ccaagccatc tagagcgaat gtataatact ctaaatggat
     1141 gtattcttta gcatttttaa tatcttgtaa aacttgatca tataaatcat ttccatcaat
     1201 gaaatgatca actttattat tttcagttaa aaaaccatct tgatccatca aaagcatacg
     1261 tactaaatca tgatgttttt gaacttgttt gttatcagta ccataattac ctttatcaaa
     1321 gctttctatt tgttgtttta aaagtccatc gaaatccgtt aacaccttac cattgttttt
     1381 attcaattta cgtgccgaaa cggttctacc aaaaaacaag taaagaataa aaccaatcaa
     1441 tggtaagaca aaaagtacaa atagccatgc ccaagttgaa ctcgctgtac gtctatttct
     1501 ttctaaaaag ataataataa aagccaatac taaattaatg atgaatccaa tggctaaaat
     1561 aatggtaaaa agtgttccta aatcatttga aaatgtatat cgcatagtct tttctcctat
     1621 aaagaaaggc acaaaaaaca ttttgcacct ttcacatcat attatttatt cacagataaa
     1681 gttaaaattg cattgaattc ttcttcatta tttgggaat
//
LOCUS       DAMZKC010000287         1699 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.291, whole genome shotgun sequence.
ACCESSION   DAMZKC010000287 DAMZKC010000000
VERSION     DAMZKC010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.291"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            64..1362
                     /locus_tag="POB55_002703"
     CDS             64..1362
                     /locus_tag="POB55_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDI6501610.1"
                     /translation="MTKHEQILDYIESLSIGSKISVRKIAKFLNVSEGTAYRAIKDAD
                     KMGMVATIDRVGTVRIEKRNRNEIEHLTFNEIVNIIDGQVLGGNKGITKMVSKFAIGA
                     MELKDILKYIGPKTLLIVGNREDVQIEALKRGTAILITGGFKPSNKVINFANEHDLPV
                     LSSSYDTFLVANIINKALFNQKIRKDILIVQDIMTPLDDLSVLFDTMKIADYKRMANR
                     TGHTRFPVVNESYKLVGIVTSREMINTKDDDEIDKVMTRNPIYVNAMSTVASCAHMMI
                     WEGIELIPVVSSNKKTVGVINRQDVLKSMQLLGRQPQMGETINDQIAKYITMNQDGIT
                     VEVSPLLINHYGTVSKAAFVSIIEETIQYEMRKFKKGNVMIENLNIVYIKTVPIESHI
                     TVRFGILDVGRNFAKIEVNMHSQNDKVASALVICQMFDEV"
BASE COUNT          606 a          238 c          337 g          518 t
ORIGIN      
        1 atgcttttta tccaaaataa agttataatg tagttaatac tgttcgaaaa gaagaggtaa
       61 agaatgacaa aacatgaaca aattttagat tatattgaat cgttatctat aggctctaaa
      121 atttcggtgc gtaaaatcgc taaattttta aacgtatctg aagggacagc atatcgtgca
      181 attaaagatg ctgataaaat gggcatggtc gcaacgatag atcgtgttgg tacggttcga
      241 atcgaaaaac gtaatcgtaa tgaaattgaa catttaactt ttaatgaaat tgtcaatatt
      301 attgatggcc aagtgttagg tggcaataaa ggtatcacta agatggtatc taaatttgca
      361 attggagcaa tggaactcaa agatatttta aaatatattg gtccaaagac attattgatt
      421 gtcggaaatc gtgaagatgt gcagattgaa gctttaaaaa gaggtacggc tatattaatt
      481 actggtggat ttaagccatc taataaagtt attaattttg cgaatgaaca tgatttacct
      541 gttctatcat caagttatga tacatttcta gttgcaaata ttatcaacaa agcacttttt
      601 aatcagaaaa tacgaaaaga tatcttaata gttcaagata taatgacgcc attagatgat
      661 ttatctgtgc tgtttgatac gatgaaaata gcagattata agcgtatggc gaatcgaact
      721 ggccatacaa gatttccggt agtcaatgag tcttataaat tagtaggtat tgtaacaagt
      781 cgagagatga ttaatacaaa ggacgacgac gaaatagata aagtgatgac gcgaaatcct
      841 atttatgtaa atgctatgag tacagtagca agttgtgcgc atatgatgat ttgggaaggt
      901 attgaattga ttcctgtcgt ttcgagtaat aaaaagacag tgggtgtgat taatagacaa
      961 gatgttttaa agtctatgca acttttagga agacagcctc agatgggtga aacaattaac
     1021 gatcaaatcg ccaaatatat aacaatgaat caagatggaa ttacagtaga agtttcacca
     1081 cttttaatca atcattacgg aacagtaagt aaagcggcat ttgtaagtat tatagaagaa
     1141 acaatacaat atgaaatgcg taaatttaaa aaaggtaatg tcatgataga gaacttaaat
     1201 atcgtgtata ttaaaacagt acctattgaa tcacatataa cagtgcgctt tggcatacta
     1261 gacgtcggaa gaaacttcgc caaaatagaa gtgaacatgc atagtcaaaa cgacaaagta
     1321 gcgagcgctc tcgtgatatg ccaaatgttt gacgaagtat aggaacttag cagagaaaat
     1381 aggcataaga tagatttcgc acaaacgttg gggcccaact tgaacattat agtaagctga
     1441 cattacgtca gcttctatgt tggggcccac cccaacttgc atggtctgta gaatttcttt
     1501 tcgaaattct ctatgttggg gccccacccc aacttgcatt gtttgtagaa attgggggtc
     1561 caatttctct atgttgtggc ccctgaatta aataaagctt gttatttgtg catttttatt
     1621 tggtcaactg ttgctagtat aatattgctt gatgtcctat aatcgttatg aactaaatag
     1681 tatgaaaata tatatatct
//
LOCUS       DAMZKC010000288         1697 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.292, whole genome shotgun sequence.
ACCESSION   DAMZKC010000288 DAMZKC010000000
VERSION     DAMZKC010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.292"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..701
                     /locus_tag="POB55_002704"
     CDS             <1..701
                     /locus_tag="POB55_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiopeptide-type bacteriocin biosynthesis
                     protein"
                     /protein_id="HDI6501611.1"
                     /translation="IEKYFLMRYIKQGDILKLRLFRNDENYAEIYSILKNWLPHVRQT
                     TEVSDYEFVSYEPEFFRYGGKNTINEIEAFFEYDTNLAVNIIENDFKFDRPYIVAISI
                     MYLFEMFSISNEERMEIVNNYVPTSFKSKDIRPFKNELVTICNPANNFEYMAKHYSGI
                     YRILKDGNQILSKLNEGLKKTLTTKRSRIIGSLIHMRCNRIFGIDKDQETFVLSIVKE
                     IVKTQKHWCGDKND"
     gene            694..>1697
                     /locus_tag="POB55_002705"
     CDS             694..>1697
                     /locus_tag="POB55_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase C family protein"
                     /protein_id="HDI6501612.1"
                     /translation="MIDMNLILKKKFQEISEVDDFISKASTETDYFEPSTLSHGIPGI
                     ILFLDAYQKVFDINTEQIVHKYIMKLAPYLQSGQYNNSLFGGLSGIAFSMDLASQNGK
                     NYQNILNNIDGHIVNEIENKFDQILQEPLNPLNYDTVSGLAGIGRYLLNRVDENEFNV
                     KALKSILVYFKDIQYSKNSWVVPQESQFLESDKNYFTEGNINLGLAHGVLGPMSLFAL
                     CVIKGITIENHQHILKDMYKFIMDEKFCNHERWLQRYDLISERNHFNFIRNGWCYGNT
                     GVMTTLFLIGQALQDDEIIKMSKKVMLQVVNDKDENLISPTICHGLSSQILMLTIMNL
                     "
BASE COUNT          638 a          200 c          290 g          569 t
ORIGIN      
        1 atatagaaaa atatttttta atgcgttata tcaaacaagg tgacatttta aagttgaggt
       61 tattcaggaa tgacgaaaat tacgcagaaa tatacagcat cttgaagaat tggttaccac
      121 atgttcgaca aacgacagaa gtttcagatt atgaatttgt atcatacgag ccagaatttt
      181 ttagatatgg tggaaagaac acgattaatg aaatagaagc cttctttgaa tatgacacga
      241 atttagctgt caatattatt gaaaatgatt ttaagtttga tcgaccatat attgttgcta
      301 tttcaataat gtatttattt gagatgtttt caatttctaa tgaagagcgt atggaaattg
      361 ttaataacta tgtacctaca tcatttaaaa gtaaagatat tagacctttt aaaaatgaac
      421 ttgtaactat atgtaatcct gctaataatt ttgaatatat ggctaaacac tatagtggta
      481 tttatcgaat tttaaaagat ggcaaccaaa ttttgagcaa attaaatgaa gggcttaaaa
      541 aaactttaac tactaaaagg tcaaggatta ttggcagctt aattcatatg cgctgcaatc
      601 gaatatttgg cattgataag gatcaagaaa catttgtttt atctattgta aaagaaattg
      661 ttaaaactca aaagcattgg tgtggtgaca aaaatgattg atatgaatct aatattgaag
      721 aagaagtttc aagagatttc agaagtagat gatttcattt caaaagcatc tactgaaacc
      781 gattattttg aaccttcaac tttatcacat ggtataccag gtatcatttt gtttttagat
      841 gcttatcaaa aagttttcga tattaatact gagcaaattg tgcataagta catcatgaaa
      901 ttagcaccat atttacagag tggtcaatat aacaattctt tgtttggtgg actttcaggt
      961 attgcatttt caatggattt agcatcgcaa aatggcaaaa attatcaaaa tattttaaat
     1021 aatattgatg gtcacattgt aaatgaaata gaaaataaat ttgatcaaat attacaagaa
     1081 ccattaaatc cattaaatta tgatactgtc agtggattag ctgggatagg gagatatttg
     1141 ctaaatagag tagatgagaa tgaatttaat gttaaagcat taaaaagcat attagtatac
     1201 tttaaagata ttcaatattc taaaaatagc tgggtagtcc cacaagaaag tcaattttta
     1261 gagtctgata aaaattattt tactgaaggt aatatcaatc ttggccttgc acatggagtg
     1321 ctaggaccga tgtctttatt tgcattatgc gtgattaaag gaattacgat tgaaaatcat
     1381 cagcacatat taaaagacat gtacaaattt atcatggacg aaaaattttg taaccacgaa
     1441 agatggttgc agcgttacga tttaatttct gaacgtaatc atttcaattt tattcggaat
     1501 ggttggtgtt atggcaatac gggtgtaatg acgacgttgt ttttaatcgg ccaagcatta
     1561 caagatgatg aaataattaa aatgtctaaa aaagtgatgc tacaagtagt aaatgataaa
     1621 gatgaaaatt taataagtcc aactatttgt catggattgt catcacaaat attaatgtta
     1681 acaattatga atttgaa
//
LOCUS       DAMZKC010000289         1693 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.293, whole genome shotgun sequence.
ACCESSION   DAMZKC010000289 DAMZKC010000000
VERSION     DAMZKC010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.293"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(92..418)
                     /locus_tag="POB55_002706"
     CDS             complement(92..418)
                     /locus_tag="POB55_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphostatin A"
                     /protein_id="HDI6501613.1"
                     /translation="MEQFELFSIDKFKCNSEAKYYLNIIEGEWHPQDLNDSPLKFILS
                     TSDDSDYICKYINTEHKQLTLYNKNNSSIVIEIFIPNDNKILLTIMNTEALGTSPRMT
                     FIKQKS"
     gene            complement(449..1615)
                     /gene="scpA"
                     /locus_tag="POB55_002707"
     CDS             complement(449..1615)
                     /gene="scpA"
                     /locus_tag="POB55_002707"
                     /EC_number="3.4.22.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease staphopain A"
                     /protein_id="HDI6501614.1"
                     /translation="MKRNFPKLIALSLILSLSVTPIANAESNSNIKAKDKKHVQVNVE
                     DKSIPTEVRNLAQKDYLSYVTSLDKIYNKEKASYTLGEPFKIYKFNKKSDGNYYFPVL
                     NTEGNIDYIVTISPKVTKYSSSSSKYTINVSPFLSKVLNQYKDQQITILTNSKGYYVV
                     TQNHKAKLVLKTPRLEDKKLKKTESIPTGNNVTQLKQKASVTMPTSQFKSNNYTYNEQ
                     YVNKLENFKIRETQGNNGWCAGYTMSALLNATYNTNKYHAEAVMRFLHPNLQGQRFQF
                     TGLTPREMIYFGQTQGRSPQLLNRMTTYNEVDNLTKNNKGIAVLGSRVESRNGMHAGH
                     AMAVVGNAKLDNGQEVIIIWNPWDNGFMTQDAKNNVIPVSNGDHYQWYSSIYGY"
BASE COUNT          532 a          251 c          236 g          674 t
ORIGIN      
        1 ctttttctaa aaaaatctca tcgcatgaaa ttttttcttc ctagagacct ttaataagat
       61 taatagttta cttaatcata tctagatagt cttatgactt ttgcttaatg aaagtcattc
      121 taggagaagt tcccaaagct tctgtgttca taattgttag tagtatttta ttatcatttg
      181 gtataaatat ttcaataaca attgagctat tatttttatt atataatgtg agttgtttgt
      241 gttctgtatt tatatatttg caaatgtaat cagaatcgtc tgaggtactg agaataaatt
      301 ttaaaggact atcatttaaa tcttgaggat gccattctcc ctcaataata ttaagataat
      361 acttagcctc tgaattacat ttgaatttat caatactaaa taattcaaat tgttccataa
      421 tattatttac ctttctaaaa tacaaatttt aataaccata aatagatgaa taccattgat
      481 aatggtcgcc attggatact ggaataacat tgtttttagc atcttgagtc ataaaaccat
      541 tatcccatgg attccatata attataacct cttgtccatt atctaattta gcgttcccaa
      601 caactgccat ggcatgccct gcgtgcatac catttcttga ttctactcta ctacctaaaa
      661 cagcaattcc tttattattt ttagtaagat tgtcaacttc attatatgta gtcattctat
      721 taagaagttg tggacttctt ccctgagttt gtccaaaata aatcatctct cttggcgtta
      781 aaccagtaaa ttggaatcgt tgtccttgta agtttgggtg taaaaatctc atcacagctt
      841 ctgcatgata tttgttagta ttataagtcg catttagtaa tgcagacatc gtatagcctg
      901 cacaccaacc attgttacct tgagtttctc ttatcttgaa attctcaagt ttatttacat
      961 attgctcgtt gtaagtataa ttattacttt taaattgact agttggcata gtgacagaag
     1021 ctttttgctt tagttgcgtt acattattgc cagtaggtat actctcagtc ttttttaact
     1081 ttttatcttc tagacgtggt gtttttagta ctagtttagc tttatgattt tgagtaacca
     1141 catagtaacc ttttgagttt gtcaaaatag taatttgttg atctttatat tgatttaaga
     1201 ctttagataa aaatggagaa acattgattg tgtatttaga agatgaacta gaatacttag
     1261 tgacttttgg tgaaattgta actatataat cgatattacc ttcagtgttc aaaactggaa
     1321 aataataatt accatcactc tttttgttga atttataaat tttaaaaggt tcaccaagtg
     1381 tatacgaggc tttctcttta ttatagattt tatcaagtga agtaacataa gaaagatagt
     1441 ctttttgcgc taaattacgt acttcagtag gaatactctt gtcctcgaca ttaacttgca
     1501 catgcttctt atcttttgct ttgatatttg aattgctctc agcgtttgca ataggggtaa
     1561 cacttaaaga aagtattaaa cttaaagcta ttaattttgg aaagtttctt ttcatataaa
     1621 aactccttta ttttattata aaataattat attaaattta gttaagagtt tcaatataaa
     1681 tttttaatct atg
//
LOCUS       DAMZKC010000290         1600 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.294, whole genome shotgun sequence.
ACCESSION   DAMZKC010000290 DAMZKC010000000
VERSION     DAMZKC010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.294"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(53..961)
                     /gene="rocF"
                     /locus_tag="POB55_002708"
     CDS             complement(53..961)
                     /gene="rocF"
                     /locus_tag="POB55_002708"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HDI6501615.1"
                     /translation="MTKTKAIDIIGAPSTFGQRKLGVDLGPTAIRYAGLISRLKQLDL
                     DVYDKGDIKVPVVNIEKFHSEQKGLRNYDEIIDVNQKLNKEVSASIENNRFPLVLGGD
                     HSIAVGSVSAISKHYNNLGVIWYDAHGDLNIPEESPSGNIHGMPLRILTGEGPKELLE
                     LNSNVIKPENIVLIGMRDLDKGERQFIKDHNIKTFTMSDIDKLGIKEVIENTIEYLKS
                     RNVDGVHLSLDVDALDPLETPGTGTRVLGGLSYRESHFALELLHQSHLISSMDLVEVN
                     PLIDSNNHTAEQAVSLVGTFFGETLL"
     gene            complement(1099..1171)
                     /locus_tag="POB55_002709"
     tRNA            complement(1099..1171)
                     /locus_tag="POB55_002709"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1136..1138),aa:Lys,seq:ttt)
     gene            complement(1175..1249)
                     /locus_tag="POB55_002710"
     tRNA            complement(1175..1249)
                     /locus_tag="POB55_002710"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1215..1217),aa:Gln,seq:ttg)
     gene            complement(1259..1342)
                     /locus_tag="POB55_002711"
     tRNA            complement(1259..1342)
                     /locus_tag="POB55_002711"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1306..1308),aa:Tyr,seq:gta)
     gene            complement(1372..1447)
                     /locus_tag="POB55_002712"
     tRNA            complement(1372..1447)
                     /locus_tag="POB55_002712"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1412..1414),aa:Val,seq:tac)
     gene            complement(1455..1526)
                     /locus_tag="POB55_002713"
     tRNA            complement(1455..1526)
                     /locus_tag="POB55_002713"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1491..1493),aa:Glu,seq:ttc)
BASE COUNT          495 a          346 c          256 g          503 t
ORIGIN      
        1 aaaaaagtag tgcaaaaagc aaaatatact ttatacacta caaatcattt atttataata
       61 aagtttcacc aaaaaatgtt ccaactaatg aaaccgcttg ttcagcagta tgattattac
      121 tgtcaatcaa tggatttact tcaactaaat ccattgagga aattaaatgt gattgatgca
      181 gtaattccaa tgcaaaatgg ctttctctat aactaagacc acccaaaact ctagtaccag
      241 tgcctggcgt ttcaagcgga tctaaagcat caacatctaa agataaatga acgccatcaa
      301 cattgcgtga cttcaaatat tctattgtat tttcaattac ttcctttatc cccaatttat
      361 caatatctga catagtaaat gttttaatat tatgatcttt gataaattgt ctttcacctt
      421 tatctaaatc tctcatacca attagtacga tgttttctgg cttgattaca ttactattta
      481 attctaaaag ttctttggga ccttcgcctg tcaaaatcct tagaggcata ccatgaatat
      541 ttccacttgg tgactcttca ggtatattta aatcaccatg tgcatcatac caaataacac
      601 ctaaattatt ataatgttta cttattgctg atactgaacc taccgcaata gaatgatctc
      661 caccaagaac tagaggaaat ctgttatttt caattgatgc tgaaacctct ttatttaatt
      721 tttgattaac atctataatt tcatcataat ttcttaatcc tttttgttca ctatgaaatt
      781 tttcaatgtt cacaacaggt accttaatat cccccttgtc atatacatca aggtctaatt
      841 gctttaatct tgaaattaat ccagcatatc taattgctgt tggtcctaaa tcaacaccta
      901 attttctttg tccaaatgtt gatggtgcac ctataatatc aattgctttt gtctttgtca
      961 taagcgtccc cctttgctct atgtaattaa agaataatgt atgcgcttac cattatcaag
     1021 caatagctac acatataatc tgtttatctt attacttcat aaaaaaaagc acttcccaaa
     1081 aatggaaagt gcaagtagtg agccatagag gattcgaacc tctgaccctc tgattaaaag
     1141 tcagatgctc taccaactga gctaatggct ctaatggctg ggctagctgg attcgaacca
     1201 acgagtgacg gagtcaaagt ccgttgcctt accgcttggc tatagcccaa tatatagatg
     1261 gtggaggggg gcagattcga actgccgaac ccgaaggagc ggatttacag tccgccgcgt
     1321 ttagccactt cgctacccct ccagcttatt catataattt aataatcaaa atggtggaga
     1381 atgacgggtt cgaaccgccg accctctgct tgtaaggcag atgctctccc agctgagcta
     1441 attctccaaa ataatgactc ctacgggact cgaacccgtg ttaccgccgt gaaagggcgg
     1501 tgtcttaacc gcttgaccaa ggagccatgg ctccacaggt aggactcgaa cctacgaccg
     1561 atcggttaac agccgatagc tctaccactg agctactgtg
//
LOCUS       DAMZKC010000291         1596 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.295, whole genome shotgun sequence.
ACCESSION   DAMZKC010000291 DAMZKC010000000
VERSION     DAMZKC010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.295"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            231..947
                     /locus_tag="POB55_002714"
     CDS             231..947
                     /locus_tag="POB55_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDI6501616.1"
                     /translation="MGRKWNNIKEKKAQKDKNTSRIYAKFGKEIYVAAKSGEPNPESN
                     QALRLVLERAKTYSVPNHIIEKAIDKAKGAGDENFDHLRYEGFGPSGSMLIVDALTNN
                     VNRTASDVRAAFGKNGGNMGVSGSVAYMFDHVATFGIEGKSVDEILETLMEQDVDVND
                     VIDDNGLTIVYAEPDQFAVVQDALRAAGVEEFKVAEFEMLPQTDIELSEADQVTFEKL
                     IDALEDLEDVQNVFHNVDLK"
     gene            947..1420
                     /locus_tag="POB55_002715"
     CDS             947..1420
                     /locus_tag="POB55_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDI6501617.1"
                     /translation="MKSAEQWIDELQLESHPEGGFYRETIREVLKDGRRAPFSSIYFL
                     LTDDNISHFHRIDADEVWYYHAGDSLTIHMINPDGEYTTATLGTDIQNGDVLQYVVPK
                     GTIFASSIEFSNTYSLVGCMCQPAFEFKQFELFKQSELITQYPHLKSVIEKYALK"
     gene            1512..>1596
                     /locus_tag="POB55_002716"
     CDS             1512..>1596
                     /locus_tag="POB55_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501618.1"
                     /translation="MLQLFLMKIALNDFENDKSVLFIFLKNH"
BASE COUNT          568 a          198 c          320 g          510 t
ORIGIN      
        1 atgctttatt tgaagatgta gaacaatttt taaatattta attgaaattg agtgtcgaaa
       61 gcatagaatt tgcttatcgg cacattttta atttatacat attttaaaac taagtaacag
      121 tttgaaggaa tcgtagttca ataatgttaa ttgtgaaaat gtatataaac ataaaaaaat
      181 catgtataat atatgttgtt aattaaacag ttcgaaagcg agatgacatt atgggacgta
      241 aatggaataa cattaaagaa aaaaaggccc aaaaagataa aaacacaagt agaatatatg
      301 cgaaatttgg taaggagatt tatgttgcag caaaatctgg tgaacccaat ccagaatcta
      361 accaagcttt aaggttggtg cttgaacgcg ctaagacata ttcagtgccg aatcatatta
      421 ttgaaaaagc aatagataaa gctaagggtg ctggagacga aaactttgat cacctaagat
      481 atgaaggatt tggcccaagc ggatcaatgc taattgttga tgcgttaaca aataatgtaa
      541 atcgtactgc ctctgatgtg cgagctgctt ttggtaaaaa tggcggtaat atgggtgtat
      601 ctggatcagt tgcttatatg tttgatcatg tggcaacatt tggtattgaa ggaaagtctg
      661 ttgacgaaat acttgaaaca ttaatggaac aagatgtaga tgtaaatgat gtgattgacg
      721 ataatggatt gacaatagtc tatgctgaac cagatcaatt tgcagtcgtt caagatgcgc
      781 ttcgtgcagc aggtgttgaa gaatttaaag ttgctgaatt tgaaatgtta cctcaaacag
      841 atattgaact ttctgaagcg gaccaagtaa catttgaaaa attaatcgat gcattagaag
      901 atttagaaga tgtacaaaac gtattccata atgtggattt gaaataatga aatcagcaga
      961 acaatggatt gatgaattgc aacttgaatc acatcctgaa ggtggtttct atagagagac
     1021 aattcgagaa gtattgaaag atggacgcag agcgccgttt agtagtattt attttttact
     1081 tacagatgac aatatttcgc attttcatcg aattgatgct gatgaagtat ggtactatca
     1141 tgctggcgat tctctaacaa ttcatatgat aaatccggat ggggaatata cgactgcaac
     1201 attgggtact gatatccaaa atggagatgt attgcaatat gtagtgccta aaggaacaat
     1261 ttttgcttct tcaatcgaat tttcaaatac ttatagttta gtaggttgta tgtgtcaacc
     1321 ggcatttgag tttaagcagt ttgaattgtt taagcaatct gaattaatta cacaatatcc
     1381 gcatcttaaa tcagtgattg aaaaatatgc tttaaaataa aaatgatcaa tgaagtggtt
     1441 tgaaggttgt taataaacct ttgagtcact tcatttttgt atgtattctt gattgaatca
     1501 gaatagaatt gatgctacag ctgtttttaa tgaaaatagc attaaatgat tttgaaaacg
     1561 ataagagtgt gttatttata tttttgaaaa atcact
//
LOCUS       DAMZKC010000292         1582 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.296, whole genome shotgun sequence.
ACCESSION   DAMZKC010000292 DAMZKC010000000
VERSION     DAMZKC010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.296"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..212
                     /locus_tag="POB55_002717"
     CDS             <1..212
                     /locus_tag="POB55_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015977661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501619.1"
                     /translation="SSFGIYNPSYLHAPIDKQLDKPEVMIANSSLEFLYKFELEPAFI
                     KIVSNQRINKLKKYKLISTDSYEHN"
     gene            complement(313..492)
                     /locus_tag="POB55_002718"
     CDS             complement(313..492)
                     /locus_tag="POB55_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501620.1"
                     /translation="MEQTIKQFLEFRKQFTPAQWHEINRIIDGQFSKKAAELQLDDQD
                     VEVIKNIITQQKIMK"
     gene            complement(506..742)
                     /locus_tag="POB55_002719"
     CDS             complement(506..742)
                     /locus_tag="POB55_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002469095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI6501621.1"
                     /translation="MPIDTKLLKSKMALKEHNIKTLSEEIGVNRDTLSNMIHGRTKPS
                     YPVINGIYFALELTPQEGRDIFFNEDLRKKKVLT"
     gene            894..1208
                     /locus_tag="POB55_002720"
     CDS             894..1208
                     /locus_tag="POB55_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012991010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI6501622.1"
                     /translation="MCFSKRMKQSREKQGMTLAELGRKIGKTEATVQRYESGNIKNLK
                     NDTIESIATALNVNPAYLMGWVEENDDEVQHRAAHLEGELTDDEWQRVLDYADYIRSK
                     RK"
     gene            1221..>1582
                     /locus_tag="POB55_002721"
     CDS             1221..>1582
                     /locus_tag="POB55_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000525002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HDI6501623.1"
                     /translation="MGLYEETLIQHDYIEIREADVLPDNLDGVWLGDLILIKRGLSDR
                     EKAGILFEELAHNKLTYGDIADYSKFNNRKFENYARRHGFISAVPLREIVEAYNYGVR
                     NLYELSEYLQLSEEYILEA"
BASE COUNT          537 a          229 c          284 g          532 t
ORIGIN      
        1 attcatcttt cggtatatat aatccaagtt atttgcatgc tccaatcgat aaacaattag
       61 ataaaccaga agttatgata gctaattcgt cattagagtt tttatataaa ttcgaattag
      121 aacctgcgtt tataaagatt gtttctaatc aacgaataaa caaacttaaa aagtataagt
      181 taatctctac cgattcttac gagcataatt aatatcgcta aattcatgaa aaagtgaatt
      241 gctagtagtg tgttggtcag catcattttg tatcttcctt tcgtgatttt tgaataactt
      301 ttattcaaat tgttacttca taatcttttg ttgagtaata atatttttaa taacctcaac
      361 atcttggtcg tcgagttgta gctcggcggc ttttttacta aattgtccgt caataattct
      421 gttgatttca tgccactgtg cgggtgtgaa ttgctttcta aattctaaaa attgtttgat
      481 tgtttgttcc atttgttgtt cctccttaag ttaaaacttt ctttttgcgt aagtcttcgt
      541 taaaaaaaat atctcttcct tcttgaggtg tcaattctaa cgcaaaataa ataccattta
      601 ttaccgggta ggatggtttt gttctcccgt gtatcatgtt agataaagta tctctattga
      661 caccaatttc ttcagaaagg gttttgatgt tatgttcttt caaagccatt ttagatttca
      721 aaagtttagt atctataggc atttcttttc accacctttc gcattacgta agtaatctta
      781 tcatgatgtt acaaaagagg tcaagcattt tacgaaagtt ttttagaaaa atattgcaaa
      841 taccgaaagt tttccttata atagaactat caagtaaaag gagctgtatt acgatgtgct
      901 tttcaaaaag aatgaaacaa tcaagagaaa aacaaggtat gactttggcc gaactaggaa
      961 gaaaaattgg taaaactgaa gctactgtac aacgttatga aagcggaaat atcaaaaatc
     1021 taaaaaacga tactatagaa agtatagcta ctgcattaaa tgttaatcct gcgtatttaa
     1081 tggggtgggt tgaagaaaac gatgatgaag tacaacatcg tgcagctcat cttgaaggag
     1141 aattgacaga tgacgaatgg caaagagttt tagattatgc agattatata agaagcaaac
     1201 gtaagtaaag gatgtatcag atgggattat atgaagaaac tttaatacaa catgattata
     1261 ttgaaataag agaggctgat gtgcttccag ataatttaga cggggtatgg ttaggagatt
     1321 taattttgat aaagcgtggt ttatcagata gagaaaaggc agggattctc tttgaagaat
     1381 tagcacataa taaacttaca tacggtgata tagccgatta ctcgaaattc aacaatcgca
     1441 agttcgaaaa ttacgcaaga cgacacggct ttatctcagc tgttccatta cgcgaaattg
     1501 tagaagctta taattatggc gtacgtaact tgtatgagtt gtctgagtat ctacaattaa
     1561 gcgaagaata catattagaa gc
//
LOCUS       DAMZKC010000293         1580 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.297, whole genome shotgun sequence.
ACCESSION   DAMZKC010000293 DAMZKC010000000
VERSION     DAMZKC010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.297"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(319..519)
                     /locus_tag="POB55_002722"
     CDS             complement(319..519)
                     /locus_tag="POB55_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDI6501624.1"
                     /translation="MNNGTVKWFNAEKGFGFIEREDGSDVFVHFSAIAEDGYKSLEEG
                     QKVEFDIVEGDRGEQAANVVKM"
     gene            complement(876..1562)
                     /locus_tag="POB55_002723"
     CDS             complement(876..1562)
                     /locus_tag="POB55_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HDI6501625.1"
                     /translation="MTEYLLSAGICMAIVSILLIGMAISNVSKGQYAKRFFFFATSCL
                     VLTLVVVSSLSSSANASQTDNGVNRSGSEHPTVYSATSTKKLHKEPATLIKAIDGDTV
                     KLMYKGQPMTFRLLLVDTPETKHPKKGVEKYGPEASAFTKKMVENAKKIEVEFDKGQR
                     TDKYGRGLAYIYADGKMVNEALVRQGLAKVAYVYKPNNTHEQLLRKSEAQAKKEKLNI
                     WSEDNADSGQ"
BASE COUNT          481 a          299 c          200 g          600 t
ORIGIN      
        1 tgtggtatcc tattattgtt aaacattttg agtgtttaac attacttctt gttatcgcca
       61 tatacagact ttaattctcg tcattattaa agttctgtat tggcgctttt ttatattcaa
      121 aatcaaaagt cggacagatg aaaagtaaaa aacttttcca tcagttcgat ctattttaga
      181 accattatat attgttaaat ctccacctaa atttattgga caaaaaaata acggctagtg
      241 cttttaccac accaactgtt atcgatttaa agcattaaaa aacagcccta taataaggac
      301 tgtttgaata agttaaaatt acattttaac tacgtttgca gcttgctcgc cacggtcgcc
      361 ttcaacgatg tcgaattcaa ctttttggcc ttcttctaat gatttgtatc catcttcagc
      421 gattgctgag aagtgtacga atacgtcgct accatcttct ctttcgatga aaccaaaacc
      481 tttttctgca ttaaaccatt taactgtacc gttattcata ttgaatacct ccgtcgtgct
      541 tttgcactaa atattttgta acaaattcat aaatgaaaag gagaatattc tatataaata
      601 actcacattt ttcttcacgc tctttattac ttaactttca ttatacactt ttaaatatag
      661 aagtaaagca ttattttatt ttctgtgata atacacattg aactatatag taatattcca
      721 ttttaatact aaatttaaaa tttttatttt ctaatattaa atacacttgc ttttgatact
      781 atttactttt ttaattctga attgataata aatcatatcg tgatccaaaa attataaaag
      841 tgccactagc agcagtgaca cttttacaat gagcattatt gacctgaatc agcgttgtct
      901 tcgctccaaa tatttaattt ctcttttttc gcttgtgctt cactttttct taaaagttgt
      961 tcatgtgtat tgttaggttt ataaacataa gcaactttag ccaagccttg acgaactaaa
     1021 gcttcgttta ccatttttcc atcagcataa atatacgcta agccacgtcc atatttatca
     1081 gttctttggc ctttgtcaaa ctcgacttca attttctttg cattttctac catttttttc
     1141 gtaaatgcac ttgcttcagg accatatttc tctacacctt ttttaggatg ctttgtttca
     1201 ggtgtatcca ccaataatag tctaaatgtc attggttgac ctttgtacat taatttaacc
     1261 gtatcaccat caatcgcttt aattaatgtc gcaggttctt tatgtaattt tttagttgaa
     1321 gttgcactat atactgttgg atgttcagaa ccacttctat ttacgccgtt atctgtttgt
     1381 gatgcatttg ctgagctact tagacttgaa actacaacta aagttaacac taagcaacta
     1441 gtagcgaaaa agaaaaacct ctttgcgtat tgccctttcg aaacattact gatagccatc
     1501 cctataagta atattgaaac aattgccata catatgccag cacttaataa gtattctgtc
     1561 ataactaaca cctctttctt
//
LOCUS       DAMZKC010000294         1518 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.298, whole genome shotgun sequence.
ACCESSION   DAMZKC010000294 DAMZKC010000000
VERSION     DAMZKC010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.298"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(325..519)
                     /locus_tag="POB55_002724"
     CDS             complement(325..519)
                     /locus_tag="POB55_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501626.1"
                     /translation="MRIEDAYRKDVIITLLNNEEYEGVVTDYENEFESETGNLVVDIQ
                     TDFAVYSFDETEIKSIRLLK"
     gene            complement(530..1177)
                     /locus_tag="POB55_002725"
                     /pseudo
     CDS             complement(530..1177)
                     /locus_tag="POB55_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065796.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
BASE COUNT          456 a          232 c          187 g          643 t
ORIGIN      
        1 gatggttgta gttattttat gaaagtgatg taatatcatt tgaattacta aatctatttt
       61 ggtaggaata tcgatgttta gtttaaatgg aatgcactat ttaagttttt aatatggaga
      121 tgtttgtgac atttcgatga ttaagatttt tataggtgtg catctttaca aataaacttt
      181 gtgtttaaaa ttgaacttag gaaatcgata ggtttagatg aagattttgt tgaaggtatg
      241 tgtcttgtat ccttagttgt tataaaagcg caaaaaatgg catcacttta tctttatctg
      301 tgtagaaagg atgctattta gaaattattt taataatcta atacttttta tctcagtttc
      361 atcaaacgaa taaacagcaa aatcagtctg tatatctaca acaagatttc ctgtttcact
      421 ctcaaattca ttttcatagt cagttacaac cccttcgtat tcttcattat tcaaaagcgt
      481 gataataaca tctttacgat atgcatcttc aattctcaat acttttcacc tacttatcaa
      541 tataaggtac tatatgagca cctgtcttcg aataatgcac ttttcctttt ctggtttcaa
      601 tatacacatt atcgatatgt acttttccaa taatttgatt aaaattaata atctctttca
      661 tatcaaatag ctcatcactt aatattagat tgcctgttga cattttttct cttaacaatt
      721 cattcaataa atctatagaa agtattgtat agctaggcaa tttcttatta tttaaaatgg
      781 ctcttttttt attttcatta tatagatgat gacctaacat atgtccattt tgtttttcac
      841 aatttatttc tattttcatt ttacaacttt caatcatttc tttcatctgg tttttagctt
      901 tttcttgtaa tttagcttct tttacttcta cctgatattt accttcacgc tctttaaaga
      961 acttgtctcg ccaactgcca acatgtggca ctgtggtact tctacactaa ggatgcatag
     1021 gtggcgcatt cacacctggt atcatatctt taactttaaa tatttttccg ttaagtgaat
     1081 gacataattt agatgtttta ctatctattt tggcaacata tttatattcg ccatcttcac
     1141 caagttcttt taaatatgtt aacttttgtg attctgcatt ttcagtaaat agttaaaaaa
     1201 gcgtataaaa atagcaccac cttctcttta tctgtgtaga aaggatgcta ttgatattta
     1261 gactttgatt gattaatttc tttttctaat tcttctttct tcgataattc atccatatgc
     1321 ttaaaaaaat cttctataga aatgtaacca tctaattgtt gaattgacat aatgtattac
     1381 tcctatttgt cctttcgttt tatatatgtt ttattataaa gttgctttag aatattatca
     1441 actatcgctt cattatttct catagttata gattgtttta tatctttcat aattttataa
     1501 acttgagact gattatat
//
LOCUS       DAMZKC010000295         1509 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.299, whole genome shotgun sequence.
ACCESSION   DAMZKC010000295 DAMZKC010000000
VERSION     DAMZKC010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.299"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(<1..321)
                     /locus_tag="POB55_002726"
     CDS             complement(<1..321)
                     /locus_tag="POB55_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDI6501627.1"
                     /translation="MNENEKNIRKNFLKFTESRYMKFVGGNDLVFSLIALVLLGIVIF
                     IFEKVSYVFDPFIIVFKTIAAPIIVSLILFYLFNPIVNMMERYRIPRVAGISIIYLAV
                     VGVIT"
     gene            complement(314..481)
                     /locus_tag="POB55_002727"
     CDS             complement(314..481)
                     /locus_tag="POB55_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501628.1"
                     /translation="MKKGLINPKLSVLHGRFVTVKGICMVSLMLVILHKWTVFIQHLM
                     NILSGGESIIE"
     gene            complement(456..1307)
                     /locus_tag="POB55_002728"
     CDS             complement(456..1307)
                     /locus_tag="POB55_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDI6501629.1"
                     /translation="MGEYIVTKTLNNNVVVCTNNDQEVILIGKGIGFNKKEGMALNDQ
                     TITIEKIYKLESEQQKAHYKSLVEIADDNVLQVIIDSLNFISNTAMNVDSKQLVVSLT
                     DHIIFAYKRLKQNQVISNPFVMETMQLYSDAYHIAKQVIDQLNAALDVHFPEDEIGFI
                     ALHIASNTEDLSMHEMTLINNVIKKGIDIIESDLVTTVDKESLQYQRFIRHVQFLIRR
                     LRRKEYIHAQDDFVSMIKNHYPICYNTAYKILTMIQKQFDVNISESEIIYLTLHIHHF
                     EERINQS"
BASE COUNT          539 a          246 c          171 g          553 t
ORIGIN      
        1 cgtaataaca cctactacag ctagataaat aatagaaata cctgcaactc ttggtattct
       61 ataacgttcc atcatattta cgattgggtt aaatagatag aatagaatta aagagacgat
      121 gataggtgct gctatcgtct taaaaacgat gataaaagga tcaaaaacat atgatacttt
      181 ttcgaaaata aaaataacaa tacccaacaa tactagcgct attaatgaga agactaaatc
      241 attcccacca acaaacttca tataccgtga ttcggtaaat tttaaaaaat tctttcttat
      301 attcttttca ttttcattca attatagatt caccaccact taaaatattc ataagatgtt
      361 gtataaaaac agtccactta tgtaaaatta ctaacatcaa tgataccata caaataccct
      421 ttacagtaac gaaacgcccg tgtaatacac ttaatttagg attgattaat cctttcttca
      481 aaatgatgaa tgtgtaatgt taaatatata atttcagact cactgatatt aacatcaaat
      541 tgtttttgta tcatagttaa aattttatat gctgtgttat agcaaatcgg atagtgattt
      601 ttaatcatag acacaaaatc atcttgtgca tgtatatatt cttttcttct taatcggcga
      661 attaaaaatt gtacgtgcct tataaaacgt tggtattgta atgattcctt atcaacagtt
      721 gtcacaaggt ctgattcaat gatatctata ccttttttaa taacattatt gatcaaggtc
      781 atctcatgca tagataaatc ttctgtatta gatgcaatat gtaatgcaat aaatcctatc
      841 tcatcttcag gaaaatgtac atctaatgct gcatttaact gatcaatcac ctgtttagca
      901 atatgatatg catcactata taactgcata gtttccataa caaatggatt gctaataact
      961 tgattttgtt ttaagcgttt ataagcaaat ataatatgat ccgttaatga aactacaagt
     1021 tgttttgaat caacattcat cgcagtatta gaaataaaat tcaacgaatc aataattact
     1081 tgtaatacat tatcatcagc gatttcaact aaacttttat aatgtgcttt ttgttgctca
     1141 ctctctaatt tataaatttt ctctattgta atagtttggt cgtttaacgc cattccctct
     1201 tttttgttaa aaccaatacc tttaccgatt aaaataactt cttgatcatt attagtacat
     1261 actacgacat tgttgttcaa tgttttagta acaatatatt ctcccattat catcacctat
     1321 ttttctatta ttaagattat atatcggaaa tgtctaactt gaaagtataa taatttaaat
     1381 acttgtgttc tattacacgc ctatcctata tgatatatct taatttaatt tcgatgcctc
     1441 tcaaagtgga ataactataa taaaaaaatc tgactcccat atttacaaat aattctattt
     1501 atttatata
//
LOCUS       DAMZKC010000296         1508 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.300, whole genome shotgun sequence.
ACCESSION   DAMZKC010000296 DAMZKC010000000
VERSION     DAMZKC010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.300"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(254..613)
                     /locus_tag="POB55_002729"
     CDS             complement(254..613)
                     /locus_tag="POB55_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HDI6501630.1"
                     /translation="MNIISTILIIFVALEFFYIMYLETIATTSKKTSETFNISVDKLK
                     DKNINLLLKNQGVYNGLIGILLIYGLFISSNPKEICAAILVYIIGVAIYGGLSSNISI
                     FFKQGTLPVLALISMLW"
     gene            complement(632..1477)
                     /locus_tag="POB55_002730"
     CDS             complement(632..1477)
                     /locus_tag="POB55_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDI6501631.1"
                     /translation="MNIMLTGATGHLGTHITNQAIANHIDHFHIGVRNVEKVPEDWRG
                     KVPVRQLDYFNPESMVEVFKGMDTVVFIPSIIHPSFKRIPEVENLVYAAKQSGVAHII
                     FIGYYADQHNNPFHMSPYFGYAARLLATSGIDYTYVRMAMYMDPLKPYLPELMNMHKL
                     IYPAGDGRINYITRNDIARGVIAIIKNPDTWGKRYLLSGYSYDMKELAAILSEASGTE
                     IKYEPVSLETFAEMYDEPKGFGALLASMYDAGARGLLDQESNDFQQLVNDQPQTLQSF
                     LQENI"
BASE COUNT          486 a          301 c          224 g          497 t
ORIGIN      
        1 tctactttac agacgtttat agctattagt atttatcagc taaaaattaa atcagatatg
       61 ggttttcgtc attaaataat ttttctataa ggaaacagag gcaacgttac tgtcacctct
      121 gcttgaaata attaggatga aatactctga gtctaaagtc cacacccaat tcgctcacat
      181 cctccaaacc tccaaacctc caaacctcca aacctccaaa cctccaaacc tccaaacccc
      241 caaacaccaa tatttaccaa agcattgata tgagtgccaa tactggcaat gtaccttgtt
      301 tgaaaaagat actaatattg cttgaaaggc caccataaat agcaacgcca atgatataca
      361 ctaaaatagc tgcgcatatt tcttttggat tactgctgat aaacaaaccg tatattagca
      421 aaattccgat taaaccgtta tatacgcctt ggttcttcaa aagtaggtta atatttttgt
      481 ctttcaattt atcgacgctt atattaaatg tctcgctagt ctttttggaa gttgtagcaa
      541 tcgtttcaag gtacataata tagaaaaact ctaatgccac aaatatgatt aaaattgttg
      601 agatgatatt cactgtaacg ctcctttatt attaaatatt ttcttgtaaa aatgattgca
      661 gtgtctgtgg ttgatcattg actaattgtt ggaaatcatt ggattcttgg tctaatagtc
      721 ctcttgctcc tgcgtcgtac attgatgcca ataatgcacc aaagccttta ggttcatcgt
      781 acatttctgc aaatgtctct aatgaaacgg gctcatattt aatttctgtg cctgatgcct
      841 cagataaaat tgcagcaagt tctttcatat cataactgta gcctgataat aaatagcgtt
      901 tgccccaagt atctggattt ttaataatag caatgacacc tctagcaata tcatttctag
      961 taatataatt aatacgacca tcaccagctg gataaatcag tttatgcata ttcatcaatt
     1021 ctggtaaata tggtttaagt ggatccatgt acattgccat tcttacatag gtatagtcaa
     1081 taccacttgt tgccaataga cgtgctgcat aaccaaaata aggactcata tggaatggat
     1141 tattatgctg atctgcgtaa taacctatga aaatgatatg agcaacgcca ctttgctttg
     1201 ccgcatatac taaattttcc acttctggaa tacgtttgaa tgatggatgg ataatacttg
     1261 gaataaacac aacggtatcc attcctttaa atacctctac catgctttcg ggattaaaat
     1321 aatctaattg tcgaacagga acttttccgc gccaatcttc tggaactttc tcaacatttc
     1381 taacaccaat gtgaaaatga tctatgtgat ttgcaatggc ttgatttgta atatgtgtgc
     1441 ctaaatgacc tgtagcacct gttaacataa tattcattca cttcatctcc taatctttat
     1501 atacataa
//
LOCUS       DAMZKC010000297         1506 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.301, whole genome shotgun sequence.
ACCESSION   DAMZKC010000297 DAMZKC010000000
VERSION     DAMZKC010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.301"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            107..742
                     /locus_tag="POB55_002731"
     CDS             107..742
                     /locus_tag="POB55_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 domain-containing protein"
                     /protein_id="HDI6501632.1"
                     /translation="MLCESKVINKNPKYRVIKYGDEYLMIDLVSTWLTLFLPMINWLI
                     PKKYVKISKKEFDDLNIVKPVKNKAFWPVAGSTILFGVTFRKYIPSLNIQLEKNMVIV
                     ICCAIFLGVLILFLFLNRKLRLEIYNNNSSKGKIILFPSLKNFCFTIFYYFLFGGLSI
                     MALSMLLTLNSQNIIGFIGWLVMTAGFFLLNMSSIIDKKIYVLSKTNTVEK"
     gene            977..>1506
                     /locus_tag="POB55_002732"
     CDS             977..>1506
                     /locus_tag="POB55_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HDI6501633.1"
                     /translation="MLLCESKIINKNPKYRIIKYNDEYLMVDIISTWISLFFPFINWF
                     IPKEYVKISREEFENLNIVKPAKKNVFWPVAGSSALLGVALRKYTHLLDIQLDKKLVI
                     AICCITFIGILIFYVRLIKKSSLNIYNTKNKRSKIFLIPTLKNVCFTLFGYILFGGLT
                     MLFLDALLSMSYQNIIV"
BASE COUNT          558 a          169 c          234 g          545 t
ORIGIN      
        1 aaatatgtgc tcactatatg acaataaagt tcatgtatta tttaaatcaa atggaattga
       61 aaagttttaa taggaataat aaacaaagga atggtagtga taaatattgc tatgtgaatc
      121 taaagttatc aacaaaaatc ctaagtatag agttattaaa tatggtgatg aatatttaat
      181 gattgattta gtaagtacct ggttaacttt atttcttcct atgattaatt ggttaattcc
      241 aaaaaaatat gtcaaaatca gtaaaaaaga atttgacgat ttaaacattg tcaaacctgt
      301 taaaaataaa gctttttggc cagttgcagg tagtactatt ttgttcggag ttacgtttag
      361 aaaatatatt ccttcactta atattcaatt agagaaaaac atggtgattg taatatgttg
      421 tgcaatattt ctgggtgttt taatactttt tttatttctg aatcgtaagc taaggttgga
      481 aatttataat aataactcta gtaaagggaa aataatttta tttccttcat taaaaaactt
      541 ttgtttcaca atattttatt attttttatt tggcggtctt tcaataatgg ctctaagtat
      601 gttattaact ttaaattctc aaaatataat aggctttatt ggttggttgg taatgactgc
      661 aggtttcttt ctgttaaaca tgtcatcgat tattgacaaa aaaatttatg tattatctaa
      721 aactaacacg gtggaaaaat gatggtttag ctggatttac tgcaggttct atttcggcaa
      781 tacttgtata ttagaccaat caaaaaaatg aatttggaat aaaagataaa aacgattgga
      841 taggacataa actagacgtt ggtatagatg ctgtagaaaa atctgcagaa aaaacagtag
      901 atggtgttga aaatgtcatg gtgaagcttc aaaaagtatt tctaatcata taagccctaa
      961 gaaatggagc tggtaaatgt tgctatgcga atctaaaatc atcaataaaa acccaaaata
     1021 tagaattatt aaatataatg atgaatactt aatggtcgat ataataagca cttggattag
     1081 tttatttttt ccttttatta attggttcat cccaaaagaa tacgtcaaaa ttagtagaga
     1141 agagtttgaa aacttaaata ttgttaaacc tgctaaaaag aatgtttttt ggccagttgc
     1201 aggtagctct gctttgctgg gagttgcatt aagaaaatat acacatttac ttgacattca
     1261 acttgataaa aaattagtta ttgccatatg ttgcatcaca tttataggga ttttaatatt
     1321 ttatgtacgc ctaattaaaa aatcatcttt aaatatttat aatactaaaa ataaaaggtc
     1381 aaaaattttt ttaataccta cactaaaaaa tgtttgtttc acattatttg gatatatttt
     1441 atttggcgga ttgactatgc tattcctaga tgcactatta tcaatgagtt atcaaaacat
     1501 aatagt
//
LOCUS       DAMZKC010000298         1502 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.302, whole genome shotgun sequence.
ACCESSION   DAMZKC010000298 DAMZKC010000000
VERSION     DAMZKC010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.302"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..205
                     /locus_tag="POB55_002733"
     CDS             <1..205
                     /locus_tag="POB55_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000931963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501634.1"
                     /translation="IINYFIKCDVNNKYYESKQRSSLEKLALKLDKVSNQLNNENIEG
                     MKKIIEKVAEANRVIAKSSLRKN"
     gene            complement(627..1055)
                     /locus_tag="POB55_002734"
     CDS             complement(627..1055)
                     /locus_tag="POB55_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDI6501635.1"
                     /translation="MNFQVSPYFLIIENADGLKRLYEELFGAKTLYIQRIKDRPTDKQ
                     LNIDKKDLNKIDQCVLQFGDIKIMIADDTEGLPITQGNNISLCITFDHVEETKRVYDQ
                     LIEKGSEVLKAFSPEFYTEGYGYVRDPYGISLHLFTKRKG"
     gene            1135..>1502
                     /locus_tag="POB55_002735"
     CDS             1135..>1502
                     /locus_tag="POB55_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HDI6501636.1"
                     /translation="MKKSVRLYHMIEYCNEKRNFKLNDLMSEFNISRSTALRDIKEIE
                     ALGMPLYSSTGKNGGYMTIGNRNQTKIAITDEELKALVFTLSSISNVSKLPFQTEYQE
                     ILKKLYNNSNKKELINQYNE"
BASE COUNT          579 a          208 c          218 g          497 t
ORIGIN      
        1 tattataaat tattttatta aatgtgacgt taataataag tattatgaaa gtaagcaaag
       61 aagcagtctc gaaaagttag ctttaaaatt ggataaagta agtaatcagt taaacaatga
      121 aaacattgaa ggtatgaaaa agataattga aaaagtagca gaagcaaata gagttatcgc
      181 taaatcttct ttaagaaaaa attgagtgtt ttagtaagat gattgaagtt gataataaat
      241 ctactaatca aaagaaattt taaatatata ttgagttaaa acttgaaaaa cttatatcta
      301 aatgaagaaa cgggaaacca ttctgaatcc tcaccctttc ccgttccaaa atcattgata
      361 atgaaaaaca tcgcttatac ggggtattgc atattttgcc ttaagttcaa atactataca
      421 tataatagaa tcacaactaa aagcacgtca taactgtact gttcattctg tacagtaggt
      481 attaaactat taaataggta taagattaaa cgaaagccaa gatgcataat gtcatggctt
      541 tttatattga aaaaacatgc gataatttgt tcccaaatgt ccttatagtt gtaccaatat
      601 ctgtaataga acactagaaa cttggtttaa cctttccttt tcgtaaataa atgtaaagat
      661 ataccataag gatctcttac ataaccataa ccttcagtat agaactctgg gctgaatgct
      721 tttaatactt cactgccttt ttctattaac tggtcataca cacgcttagt ttcttctaca
      781 tggtcaaaag taatacaaag cgatatatta ttaccttggg ttataggtaa accttcggta
      841 tcatccgcaa tcataatttt tatatctcca aattgaagta cacattgatc aattttattt
      901 aaatcttttt tatcgatatt aagttgctta tctgtcggtc tatccttgat acgttggata
      961 tacagtgttt tagcgccaaa cagctcttca tacaaccttt tcaaaccatc tgcattttct
     1021 ataattaaaa aatagggact tacttgaaaa ttcatagttt ttcctcctta aatttgttat
     1081 aaataagtat atactgtatt agtgtcacct attgatacta aaaggggaat aataatgaaa
     1141 aaatctgtta gattatatca tatgattgaa tactgtaatg aaaaaaggaa tttcaagtta
     1201 aatgatttaa tgtcagaatt taatatttct cgtagtactg ctttaaggga tataaaggaa
     1261 attgaagcat taggaatgcc tttatatagt agtacaggga aaaatggcgg ttatatgacc
     1321 ataggtaatc gaaaccaaac gaaaatagca atcacagacg aagaattgaa agctttagta
     1381 tttacacttt caagtatctc caatgttagt aagttacctt ttcaaacaga atatcaagaa
     1441 atattaaaaa agttatataa taattcgaat aaaaaagaat tgataaacca gtataatgag
     1501 at
//
LOCUS       DAMZKC010000299         1490 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.303, whole genome shotgun sequence.
ACCESSION   DAMZKC010000299 DAMZKC010000000
VERSION     DAMZKC010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1490
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.303"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(132..1487)
                     /gene="brnQ"
                     /locus_tag="POB55_002736"
     CDS             complement(132..1487)
                     /gene="brnQ"
                     /locus_tag="POB55_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDI6501637.1"
                     /translation="MKKKLTFKENMFIGSMLFGLFFGAGNLIFPIHLGQAAGSNVFIA
                     NLGFLITAIGLPFLGIIAIGISKTSGLFEIASRVNKTYAYIFTIALYLVIGPFFALPR
                     LATTSFEIAFSPFLSPKQITLYLFIFSFVFFVIAWFFARKPSRILEYIGKFLNPVFLV
                     LLAIILLFAFIHPLGGISDAPISKQYQSNALFNGFLDGYNTLDALASLAFGIIIVATI
                     KKLGIENPTDIAKETIKSGTISIIMMGIIYTLLAIMGTLSIGHFKLSENGGIALAQIT
                     QYYLGNYGIVLLSLIVMVACLKTAIGLITAFSETFEHLFPKMNYLAIATVVSFISFLF
                     ANVGLTKIIMYSVPVLMFLYPLAIALIVLTLFSSKFHHSKLIYQCTIFFTMIAALVDG
                     LKASPEFISSTSFSQTLINFSQKYLPLSDIGMGWVVLSLIGFIIGFIIYKIKHRKIPQ
                     A"
BASE COUNT          560 a          224 c          258 g          448 t
ORIGIN      
        1 tgatttgtaa aatgaaagta ctaatatttt ttgtggataa tcattgatat gtatgtatac
       61 gattgcagtt taatgtagta tgaataaaaa atgccacaag catatgttta acatatgact
      121 gtggcatagt attatgcttg tggaatttta cgatgcttaa ttttataaat aatgaagccg
      181 ataatgaaac caatcaaact gagaacaacc cagcccatac caatgtctga taatggtaaa
      241 tatttttggc tgaaattaat caaagtttgt gagaatgatg tgcttgaaat gaactctgga
      301 ctagctttta atccatctac taatgcagca atcattgtaa agaaaatggt acattgataa
      361 ataagttttg aatgatggaa tttgctacta aataatgtta gtacaatcaa ggcaattgct
      421 aatggatata agaacattaa cactgggact gagtacataa taatcttagt taaaccaaca
      481 ttcgcgaata agaacgaaat aaagcttaca actgttgcaa tcgctaggta attcatttta
      541 gggaaaaggt gttcgaatgt ttctgaaaat gccgtaatca aaccgatggc tgtttttaaa
      601 caagcaacca taacgataag tgacaacagg acgataccgt agttacctaa gtagtattga
      661 gtaatttgcg ctaaggcaat accaccattt tcactaagtt tgaaatgacc aatacttaat
      721 gtacccatga ttgctagtag ggtataaatg atccccatca taatgatact gatagtacca
      781 gacttaattg tttctttagc gatatcagtt ggattttcga tacctaactt tttaatcgtt
      841 gcaacaatga taataccaaa tgccaatgac gctagcgcat ctaaggtatt gtatccatct
      901 aaaaagccgt taaataaggc atttgattga tattgtttac taataggtgc atcagatatg
      961 ccacctaatg gatggataaa agcaaataat aaaataattg ctaataatac taagaatacc
     1021 ggatttaaaa atttaccgat atattctaaa attcttgatg gctttctcgc aaaaaaccat
     1081 gcaatcacaa agaagacgaa gctaaaaata aataaatata aagtgatttg ctttggtgat
     1141 aaaaatggcg aaaatgcaat ttcaaatgat gtcgttgcca gtctaggtaa ggcgaaaaat
     1201 ggtccgataa ctagatataa ggcaatcgtg aaaatgtaag catatgtttt attaacacgc
     1261 gatgcaattt caaataaacc agatgtcttt gaaatgccaa tagcaatgat acctagaaat
     1321 ggtaagccaa ttgctgtaat taaaaatcct aagttagcga taaaaacgtt agaaccagca
     1381 gcttgaccca agtgtattgg gaagataaga ttgccggcac caaagaataa accaaataac
     1441 atagaaccta taaacatgtt ttctttaaat gttagtttct tcttcatata
//
LOCUS       DAMZKC010000300         1490 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.304, whole genome shotgun sequence.
ACCESSION   DAMZKC010000300 DAMZKC010000000
VERSION     DAMZKC010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1490
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.304"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            206..1165
                     /gene="hyl"
                     /locus_tag="POB55_002737"
     CDS             206..1165
                     /gene="hyl"
                     /locus_tag="POB55_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hemolysin"
                     /protein_id="HDI6501638.1"
                     /translation="MKTRIVSSVTTTLLLGSILMNPVANAADSDINIKTGTTDIGSNT
                     TVKTGDLVTYDKENGMHKKVFYSFIDDKNHNKKLLVIRTKGTIAGQYRVYSEEGANKS
                     GLAWPSAFKVQLQLPDNEVAQISDYYPRNSIDTKEYMSTLTYGFNGNVTGDDTGKIGG
                     LIGANVSIGHTLKYVQPDFKTILESPTDKKVGWKVIFNNMVNQNWGPYDRDSWNPVYG
                     NQLFMKTRNGSMKAADNFLDPNKASSLLSSGFSPDFATVITMDRKASKQQTNIDVIYE
                     RVRDDYQLHWTSTNWKGTNTKDKWTDRSSERYKIDWEKEEMTN"
BASE COUNT          568 a          210 c          230 g          482 t
ORIGIN      
        1 tgcttccttt caaattttaa ataaaagtta aaaacatatt cttaaaataa tcactcatca
       61 tcactcagta atttatcagt tgctatatta aatatactaa tataaacgat tataaatatt
      121 tgatatgtct caactgcaat attctaaatt gacatattga ttattgtttc ctcaaatcgt
      181 ttaaaaaaat agaaggatga tgaaaatgaa aacacgtata gtcagctcag taacaacaac
      241 actattgcta ggttccatat taatgaatcc tgtcgctaat gccgcagatt ctgatattaa
      301 tattaaaacc ggtactacag atattggaag caatactaca gtaaaaacag gtgatttagt
      361 cacttatgat aaagaaaatg gcatgcacaa aaaagtattt tatagtttta tcgatgataa
      421 aaatcacaat aaaaaactgc tagttattag aacgaaaggt accattgctg gtcaatatag
      481 agtttatagc gaagaaggtg ctaacaaaag tggtttagcc tggccttcag cctttaaggt
      541 acagttgcaa ctacctgata atgaagtagc tcaaatatct gattactatc caagaaattc
      601 gattgataca aaagagtata tgagtacttt aacttatgga ttcaacggta atgttactgg
      661 tgatgataca ggaaaaattg gcggccttat tggtgcaaat gtttcgattg gtcatacact
      721 gaaatatgtt caacctgatt tcaaaacaat tttagagagc ccaactgata aaaaagtagg
      781 ctggaaagtg atatttaaca atatggtgaa tcaaaattgg ggaccatatg atagagattc
      841 ttggaacccg gtatatggca atcaactttt catgaaaact agaaatggtt ctatgaaagc
      901 agcagataac ttccttgatc ctaacaaagc aagttctcta ttatcttcag ggttttcacc
      961 agacttcgct acagttatta ctatggatag aaaagcatcc aaacaacaaa caaatataga
     1021 tgtaatatac gaacgagttc gtgatgatta ccaattgcat tggacttcaa caaattggaa
     1081 aggtaccaat actaaagata aatggacaga tcgttcttca gaaagatata aaatcgattg
     1141 ggaaaaagaa gaaatgacaa attaatgtaa attatttgta catgtacaaa taaatataat
     1201 ttataacttt agccgataac ttcagaaatg atgtttttcg gctaattttt atacttaatt
     1261 tagtttaatt aacttatgat atttttaatt taaactatgt acttgatttg ctttcctgac
     1321 ttaaaaatta gactaaacat gacactatat gaggtgattt ttaaaataag aaatttgaat
     1381 tccatgttgt caaacccttt atataaaagt tttgaaattg ttcaaaactt tttgaacaca
     1441 ttatgtgtta atatatttgc agaattattt taaaatcgtt ttaaaactat
//
LOCUS       DAMZKC010000301         1484 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.305, whole genome shotgun sequence.
ACCESSION   DAMZKC010000301 DAMZKC010000000
VERSION     DAMZKC010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1484
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.305"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..>1484
                     /locus_tag="POB55_002738"
     CDS             <1..>1484
                     /locus_tag="POB55_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lantibiotic dehydratase family protein"
                     /protein_id="HDI6501639.1"
                     /translation="KIREIQKLILAYEKTEIGFGEELYKDIIHHMKALFKCKNYLQID
                     TKIDMINNYLHQDIATNISEAAYLLWLLSRNNIGFTDLKVLHNRFIEKYGFEQLVNIK
                     DLISDITGFGTTIFQEEETDGNNIVMLKQKFLHALRNNDEIVINEKDVESLINDNEIN
                     HYHAPMSADVYAELYLGRFYNQYNELIVISPLTASFNAGATFGRFHHLIDTETLAKLE
                     HEKGHYYQKMICDDNVEMISINNIPKYPRNHNVLTNHDSYEYSLNLGSSNSYSKYELT
                     LDDIYVGATFNKLYLYSSQLNKRVLFESNNMYNFLKECNLYRLLREISMESVKCIESM
                     NDVSIDSFSYSPRIRYKNVILKPAYWKINEMVLPLPKNEEWDQQFLKYQEQFNIPNIV
                     NLVYGDNKLLLNLSLANHRYLLMKEYKKHKRVRLVESFLPQSKNDHVYEIVTPIYKKS
                     SYRGPEIEIPKYKNTDIEYDKDWFALHIHIDKPSQDTFIIDNLY"
BASE COUNT          581 a          192 c          227 g          484 t
ORIGIN      
        1 aaaaattaga gaaatccaga aattgatatt agcttatgaa aaaactgaaa ttggatttgg
       61 cgaggaatta tataaagaca ttattcatca tatgaaggca ctattcaaat gtaaaaacta
      121 tcttcaaatt gataccaaaa ttgacatgat taacaattat ttacatcaag atatagcaac
      181 aaatatatct gaagcagcgt acttattgtg gcttttatca agaaataata ttggattcac
      241 agatttgaaa gttcttcaca accgttttat tgaaaagtat ggatttgaac aactcgtgaa
      301 tattaaagat ttgatttcag atataacagg tttcgggaca acaatttttc aagaagaaga
      361 aaccgatgga aataatattg taatgcttaa gcaaaaattt ttacatgcct tgagaaataa
      421 cgatgagata gtcataaatg aaaaagatgt agagtcattg attaacgata atgaaatcaa
      481 tcattatcat gcgccaatga gtgcagatgt ttatgctgaa ctttatttag gacgttttta
      541 caatcagtac aatgagctca ttgttattag tcctttaacg gcgtctttta atgctggcgc
      601 tacatttggg cgatttcatc atttaattga tactgaaact ttagcaaaat tagaacatga
      661 aaaaggacat tattatcaga agatgatatg tgatgacaat gtagaaatga tttctataaa
      721 taacataccg aaatatccga gaaatcataa tgtattaact aatcatgact catacgaata
      781 ttcattgaat ttaggaagta gtaatagtta ttcaaagtat gagcttacct tagatgatat
      841 ttatgttggt gctaccttta acaaattata tttatattct agccaactaa ataaaagggt
      901 actatttgaa tcaaacaata tgtataactt tttaaaagaa tgtaatttat atcgattgtt
      961 acgagaaatt tcaatggaat ctgtgaaatg cattgaatct atgaatgatg tgagtattga
     1021 ttcatttagt tattcaccac gaatcagata taaaaatgtc attcttaaac cagcatattg
     1081 gaaaattaac gagatggtat tacctttacc taaaaatgaa gaatgggatc aacaattttt
     1141 gaaatatcaa gaacaattca atatacctaa tatcgtgaat ttagtttatg gagataacaa
     1201 gcttctacta aatttatcat tagctaatca tagatattta ttgatgaaag aatataagaa
     1261 gcataaacgt gtacgtttag tagaatcatt tttacctcaa tcaaagaatg atcatgtgta
     1321 tgaaattgtt acaccaattt ataaaaaatc atcttatcga ggacccgaaa tcgaaattcc
     1381 aaaatataaa aatacagata ttgaatatga caaagactgg tttgcattac atattcatat
     1441 cgataagcca tcgcaagata catttatcat cgacaatttg tatc
//
LOCUS       DAMZKC010000302         1464 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.306, whole genome shotgun sequence.
ACCESSION   DAMZKC010000302 DAMZKC010000000
VERSION     DAMZKC010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1464
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.306"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..409
                     /gene="fmhC"
                     /locus_tag="POB55_002739"
     CDS             <1..409
                     /gene="fmhC"
                     /locus_tag="POB55_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FemA/FemB family glycyltransferase FmhC"
                     /protein_id="HDI6501640.1"
                     /translation="SKLNQLNMQLSSINNRISKTEELIFEDGPVLDLAAALFICTDDE
                     VYYLSSGSNPKYNQYMGAYHLQWHMIKYAKSHNINRYNFYGITGVFSNEADDFGVQQF
                     KKGFNAHVEELIGDFIKPVRPILYKFAKLIYKV"
     gene            582..1454
                     /gene="dprA"
                     /locus_tag="POB55_002740"
     CDS             582..1454
                     /gene="dprA"
                     /locus_tag="POB55_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDI6501641.1"
                     /translation="MIKLFLLKLYWAHFSTKQIHQFLMAYPNVIKEEGRKKDSYLCEW
                     VNREENVHLLRKYYAFIKLDHNDIIKELQKLKVSYITYMDSEYPVLLKEIYQFPLLLF
                     YKGNIKLINNMHHLAVVGARDSTSYTQQSLEFLLSNDKSKYLTIVSGLAQGADAMAHQ
                     IALKYNLPTIAVLAFGHQTHYPKSTLALRNKIEEKGLVISEYPPHTPIAKYRFPERNR
                     IISGLSKGVLITEAKEQSGSHITIDFALEQNRNVYVLPGSMFNPMTKGNLLRIQEGAK
                     VVLNANDIFEDYYI"
BASE COUNT          559 a          187 c          225 g          493 t
ORIGIN      
        1 atcaaaatta aatcagttaa atatgcaatt atctagtatt aataatagaa ttagtaaaac
       61 cgaagaacta atatttgaag atggacctgt tttggattta gctgctgctt tatttatatg
      121 tactgatgat gaagtttatt atctatcaag tggatcaaat ccgaaatata atcagtatat
      181 gggtgcatat catctacaat ggcatatgat aaaatatgca aaatcacata atattaatag
      241 gtataatttt tatggaataa caggcgtctt tagtaatgag gcggatgatt ttggtgttca
      301 acaatttaaa aagggtttta atgcacatgt tgaagaatta attggtgatt tcatcaaacc
      361 agtaagacca attctatata aatttgcaaa acttatttat aaggtttaat tataaagtat
      421 gttggaaatt gaaattttaa attctttcca acatactttt cactttttaa gtcagtattt
      481 ttactcctta aaataacatt acatcgacaa tgtttttgta ttagaaatgt aaaacaaaaa
      541 tagaatatga ttttgtatga ttacaaaaag gagttgattt attgattaaa ctatttttgc
      601 ttaagttata ctgggcacac ttttcgacta aacaaattca tcaattttta atggcatatc
      661 ctaatgtaat taaagaggag ggaagaaaaa aagatagtta tttatgtgaa tgggtgaata
      721 gggaagaaaa tgttcattta ttacgtaaat actatgcttt tataaaactt gatcataacg
      781 atattattaa agaactgcag aaattaaaag taagttacat tacatatatg gattctgaat
      841 acccagtgct attaaaagaa atatatcaat ttccattact tcttttctat aaagggaaca
      901 tcaaattaat aaataatatg catcatttgg cagtagtagg tgcaagagat tctacaagtt
      961 atacccaaca gtctttagaa tttttattat caaatgataa aagcaaatat ttaacaattg
     1021 tttccggcct tgctcaagga gctgatgcaa tggcacatca aatagcttta aaatacaatc
     1081 tccctacaat tgcagtttta gcctttggcc atcaaacaca ttatcccaaa agtacattag
     1141 cattaagaaa taaaatagaa gaaaaaggtt tagttatatc cgaatatcca ccacatacac
     1201 caattgctaa atatagattt cctgagcgca atagaattat cagcggtttg tcaaaagggg
     1261 ttttaattac tgaggctaag gaacaaagtg gcagtcacat cacgatagat tttgcattag
     1321 agcaaaatag aaatgtttat gttttacctg gatctatgtt taatcctatg acaaaaggta
     1381 atttattacg tatccaagaa ggtgctaagg tagtattaaa cgctaatgat atatttgaag
     1441 actactatat ttaaaactaa aaat
//
LOCUS       DAMZKC010000303         1428 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.307, whole genome shotgun sequence.
ACCESSION   DAMZKC010000303 DAMZKC010000000
VERSION     DAMZKC010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1428
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.307"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(23..877)
                     /locus_tag="POB55_002741"
     CDS             complement(23..877)
                     /locus_tag="POB55_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HDI6501642.1"
                     /translation="MNKHKKGSIFGIIGLVVIFAVVSFLFFSMISDQIFFKHVKSDIK
                     IEKLNVTLNDAAKKQINNYTSQQVSNKKNDAWRDASATEIKSAMDSGTFIDNEKQKYQ
                     FLDLSKYQGIDKNRIKRMLVDRPTLLKHTDDFLKAAKDKHVNEVYLISHALLETGAVK
                     SELANGVEIDGKKYYNFYGVGALDKDPIKTGAEYAKKHGWDTPEKAISGGADFIHKHF
                     LSSTDQNTLYSMRWNPKNPGEHQYATDIKWAESNATIIADFYKNMKTEGKYFKYFVYK
                     DDSKHLNK"
     gene            complement(1153..1377)
                     /locus_tag="POB55_002742"
     CDS             complement(1153..1377)
                     /locus_tag="POB55_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501643.1"
                     /translation="MDYAHLNLEHFFARNDDLDVIRDRADFVMINNFTNEMMYRDGQI
                     EGTIDLNQYYYKNRSNAASFIMMDYKKETK"
BASE COUNT          443 a          249 c          166 g          570 t
ORIGIN      
        1 atcataactt aactctatca aattacttat tcaaatgttt actgtcatct ttatacacaa
       61 agtatttgaa gtattttcct tcagtcttca tgttcttata aaagtcagcg ataattgttg
      121 cattactttc tgcccactta atatcagtag catattgatg ttctcctgga ttttttggat
      181 tccatctcat actatacaat gtattttgat ctgtgcttga taagaagtgc ttatgaatga
      241 aatcagcacc gcctgaaata gctttttcag gtgtatccca accatgcttt ttagcatatt
      301 ctgcacctgt tttaattggg tctttatcaa gggctcctac tccatagaaa ttgtagtatt
      361 ttttgccatc aatttcgact ccattagcta attcactttt aactgcgcca gtttctaata
      421 atgcatgtga aattaaataa acttcgttaa cgtgcttatc tttagcagct tttaagaaat
      481 catccgtatg tttcaataac gttggtctat ctactaacat acgtttaatt ctatttttat
      541 caatcccttg atactttgat aaatctaaaa attgatattt ttgcttttca ttatcgataa
      601 aagtaccgct atccattgca cttttaattt cagttgcaga tgcatctctc catgcatcat
      661 tctttttatt tgatacctgt tgactcgtat aattatttat ttgtttcttt gctgcatcgt
      721 ttaatgtaac atttaacttt tcaatcttaa tgtcggattt aacatgtttg aaaaatatct
      781 gatcggatat cattgagaaa aataaaaatg agacaacagc aaatatgaca acaagtccta
      841 ttattccaaa aatagaacct ttcttgtgtt tattcatatc cacacctctt aggtcattgt
      901 tgttatacat tcagtttgat tacgataata aagtttaaca ttattaaaac cgtttaacaa
      961 ttttatctat gcattgttac atattcataa ataaacagta atgtaaatga aacgttttaa
     1021 accatttatc aacattactt tacgttgatt tacgaatcat aaatgttttt tctttttgta
     1081 taacctttga aaacctttat atcaataatc ttattattca cgtattaaaa aacaaaaaat
     1141 tctcttgttc gtttacttag tttctttttt ataatccatc ataataaaac ttgctgcatt
     1201 tgatctattt ttataatagt actgatttaa atcaatcgta ccttcaattt gaccatcacg
     1261 atacatcatt tcattagtga agttatttat catcacgaaa tcagcgcgat ctcttataac
     1321 atctaaatcg tcgtttcgtg caaaaaaatg ttctaaattt aaatgtgcgt aatccattgt
     1381 tacctcccga ttacaaattt tgttctatct actatttagc agagtaat
//
LOCUS       DAMZKC010000304         1397 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.308, whole genome shotgun sequence.
ACCESSION   DAMZKC010000304 DAMZKC010000000
VERSION     DAMZKC010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.308"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            703..840
                     /locus_tag="POB55_002743"
     CDS             703..840
                     /locus_tag="POB55_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HDI6501644.1"
                     /translation="MMVDKATTQIQHDCGIRVLVFIKLIESYIKYSLDNSILVRLRFV
                     V"
     gene            complement(846..1160)
                     /locus_tag="POB55_002744"
     CDS             complement(846..1160)
                     /locus_tag="POB55_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501645.1"
                     /translation="MYFVLAIFTIISASVSLGYSIQACASSHNINAYYALSRSLPLFL
                     LAIFSLVIHSAIFLITISIAMILVQFLDAIVGYKSEDVFKTYGPLATSVVNLILLIVF
                     LF"
BASE COUNT          505 a          197 c          202 g          493 t
ORIGIN      
        1 ttagacacag caatgtgttc aaattttcat ctattcgtaa gttagccttc gggctgactt
       61 tttatttcca ttattcacat gttaatcttg ttgttattta ggcaggtact tcggtacttg
      121 cctatttttt tgttataatg taattacatt accagtaacc aatctggctt aaaaccacat
      181 ttccggtagt caatccggct atgcatagga cttacttgcg taaagcagta agaagctgac
      241 tgcatattta aaccacccat actagttact gggtggttgt tttttatgtt atattataaa
      301 tgatcaaacc acaccaccta ttaatttagg agtgtggttt tttatatgtg aagctaaaat
      361 aactataaat gataccattt ttgataccaa aaaataatag cctcaaaatt tcaagagaaa
      421 taacttcatt ttaaatcgca ttaaatcaac gtttctataa aaaaacgtcc ttaaaaatta
      481 gttttttcaa tcgaaatgga aggtagtatt ggatagcttt aaaccgcgtt gttaagccat
      541 tcttgacttc cgaaaatggc tattgatacc attttgatac tgaatataac aaaaagccac
      601 attactgtgg ctttttttgt tttataacta aatcggattg atagataagc tttgtactta
      661 tttatatcag ttcgattttt tgattggtgt aaaaaataat cattgatggt ggataaagcg
      721 acaacacaaa tacaacatga ttgtggcatt agagtgctgg tctttattaa attaattgaa
      781 agctacatca aatattcttt agataattcg atattagttc gattaagatt cgttgtataa
      841 gtgagttaaa ataagaaaac tattaataat attaagttca ctacagatgt tgctaatgga
      901 ccataagttt taaagacatc ttcactttta taaccaacaa tcgcatctaa aaattgaact
      961 aagatcattg caatggatat agttatcaaa aatatagcac tatgaatgac taaagaaaaa
     1021 atagctaata aaaataaagg taagcttcga ctaagtgcat aatatgcatt tatattatgg
     1081 ctagatgcac atgcttgaat tgaataacct aaacttacac tggcactgat tattgtaaat
     1141 attgctaaaa caaaatacat gttaatcctt ctttctatat ttggatataa acaagtactt
     1201 gtctaaagtt atttaaaaga taattagaat aaatttatga gaaactggtt gttatcatta
     1261 taatggtttc aaatgattat aactatgtca taaactgaat ttgttgaaat ttttcattat
     1321 gcaaatttaa taataacaaa cagctcgaac tatagcatca ttttactaat gaatgcatta
     1381 aagtaactat gactaaa
//
LOCUS       DAMZKC010000305         1382 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.309, whole genome shotgun sequence.
ACCESSION   DAMZKC010000305 DAMZKC010000000
VERSION     DAMZKC010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.309"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            76..1308
                     /locus_tag="POB55_002745"
                     /pseudo
     CDS             76..1308
                     /locus_tag="POB55_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501257.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif.; incomplete; partial in the middle
                     of a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
BASE COUNT          567 a          187 c          275 g          353 t
ORIGIN      
        1 ctcctattgt atttgtaaaa ttacatgtaa attcactgta agtaaagtgg aaaatatgga
       61 aaaaggagta tgcaaatgag agataagaaa ggaccggtaa ataaaagagt agattttcta
      121 tcaaataaat tgaataaata ttcaataaga aaatttacag ttggaacagc atctatttta
      181 attggctcac taatgtattt gggaactcaa caagaggcag aagcagctga aaacaatatt
      241 gagaatccaa ctacattaaa agataatgtc caatcaaaag aagtgaagat tgaagaagta
      301 acaaacaaag acactgcacc acagggtgta gaagctaaat ctgaagtaac ttcaaacaaa
      361 gacacaatcg aacatgaacc atcagtaaaa gctgaagata tatcaaaaaa ggaggataca
      421 ccaaaagaag tagctgatgt tgctgaagtt cagccgaaat cgtcagtcac tcataacgca
      481 gagacaccta aggttagaaa agctcgttct gttgatgaag gctcttttga tattacaaga
      541 gattctaaaa atgtagttga atctacccca attacaattc aaggtaaaga acattttgaa
      601 ggttacggaa gtgttgatat acaaagcaat ccagaggatc taaaggtatc tgaagtcact
      661 cgtttcaata ataaaagtat tggaaaaaac gaattgacag gagcattaca attaaagaat
      721 aaagtttctt ttaaaaatga ctttgagttt aacattagag tagctaataa tcatcaatcg
      781 gtaacaactg gcgctgatgg atgggggttc ttatttagta aaggtgacgg taacgaatat
      841 ttacaaaagg gaggtatatt aggtcccaaa ggaatggaaa attcagcagg atttaaaatt
      901 gatacagggt ataattttaa agatcccatg gataaagaag agaaacaggc aggacaagga
      961 tttaaagggt atggtacgtt tgtaaaaaca ggtgctgatg gaactacagc aaaggttgga
     1021 acaaacatac caacaagagg taaagcggat aattcattcc aatatgcaga taattctatt
     1081 gatacaacag atggaaaatt ccatggtcaa ttacttaata atttaaaatt agcgtacaat
     1141 gaaaaatcgg ggataatgag agccgaatat gctggtaaaa tttgggaagc taatatatca
     1201 gatttgggat tggataaatc tgaagcatat aactttttaa ttacttctag tcaaagacag
     1261 ggaacatctc aaggtgtata tgcaaatggt tggatgagaa cagatttaaa taactcaaca
     1321 tttaaactaa cgcctaatct agttaataaa acagaaacaa aaacagtaga gataccgttc
     1381 ga
//
LOCUS       DAMZKC010000306         1378 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.310, whole genome shotgun sequence.
ACCESSION   DAMZKC010000306 DAMZKC010000000
VERSION     DAMZKC010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.310"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            50..>1378
                     /locus_tag="POB55_002746"
     CDS             50..>1378
                     /locus_tag="POB55_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HDI6501646.1"
                     /translation="MKPLHLKLNNFGPFLKEEIDFSKIDNNELFLISGKTGSGKTMIF
                     DAMTYALFGKASTEQREENDLRSHFADGKQPMSVTFEFQLNHRIYKVHRQGPYIKEGN
                     TTKTNAKFDVFEMVDGKYEIRESKVISGTQFIIELLGVNADQFRQLFILPQGEFKRFL
                     ISNSREKQGILRTLFDSEKFEAIREILKEEVKKEKAQIENRYQQIDLLWQEIESFDDD
                     NIKGLLGVATQQIDKLIENIPLLQARSKEILASVNESKETAIKEFEIIEKKTLENNIL
                     KDNINQLNKNKIDFVQLKEQQPEIEGIEAKLKLLQDITNLLNYIENREKIETKIANSK
                     KDISKTNNKILNLDCDKRNIDKEKKMLEENGDLIESKISFIDKTRVLFNDINKYQQSY
                     LNIERLRTEGEQLGDELNDLIKGLETVEDSIGNNESDYEKIIELNNTITNI"
BASE COUNT          609 a          146 c          235 g          388 t
ORIGIN      
        1 aaagataaaa aatattttag aaaacgaatt gagaaaggag gattaatgaa tgaaaccatt
       61 acatttaaag ttgaataatt tcggcccctt tttaaaagaa gaaattgatt tttctaaaat
      121 tgataataat gaattgtttt taataagtgg taagactgga tcgggtaaaa caatgatttt
      181 tgatgcaatg acttatgcct tgtttggtaa agcatcaact gaacaaagag aagaaaatga
      241 tttgagaagt catttcgctg atggtaaaca gccgatgtca gtaacatttg aatttcaatt
      301 aaatcatcga atttataaag tgcatagaca aggcccttat atcaaagaag gtaatacaac
      361 aaaaacgaac gctaaatttg atgtatttga gatggtggat ggcaagtatg aaattagaga
      421 aagtaaagta atttcaggta cccaattcat tattgaatta ttaggagtaa atgcagatca
      481 attccgacaa ttgtttattt tgcctcaagg tgaattcaaa cgctttttaa tatcaaacag
      541 tcgtgaaaag caagggatat taagaacact gtttgacagt gaaaaatttg aagctatacg
      601 agaaatatta aaagaagaag taaaaaaaga aaaagctcaa atcgagaata gatatcaaca
      661 aattgacctt ttatggcaag aaattgaatc atttgatgat gacaatataa aaggcttatt
      721 aggggttgcc actcaacaga tagacaaatt gattgaaaat ataccacttt tacaagctag
      781 gtcgaaagaa atactagcat ctgtaaatga aagtaaagaa actgctatta aagaatttga
      841 aataatagaa aagaaaacat tagaaaataa tatattaaaa gataatatta atcaactcaa
      901 caaaaataaa attgatttcg ttcaattgaa agaacaacaa cctgaaatag agggaattga
      961 agctaagtta aagttgttac aagatattac aaacctattg aattatattg aaaatagaga
     1021 aaaaattgaa actaaaattg ctaatagcaa aaaagatatt tctaaaacca ataataaaat
     1081 attgaatctt gattgtgata agcgaaacat agacaaagag aaaaaaatgt tagaagaaaa
     1141 tggagattta attgaaagta aaatctcttt tattgataaa actagagtat tatttaacga
     1201 tattaataag tatcaacaaa gttatctcaa tattgaacgc ttgagaactg agggtgaaca
     1261 attaggtgat gaattaaatg atctaattaa aggtttagaa acggtcgaag attcaatagg
     1321 taataacgaa agtgattacg agaaaattat cgaactaaat aatacgataa cgaacata
//
LOCUS       DAMZKC010000307         1355 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.311, whole genome shotgun sequence.
ACCESSION   DAMZKC010000307 DAMZKC010000000
VERSION     DAMZKC010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.311"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..1318
                     /locus_tag="POB55_002747"
     rRNA            <1..1318
                     /locus_tag="POB55_002747"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          358 a          302 c          399 g          296 t
ORIGIN      
        1 cactgccgag aaaagcctct agatagaaaa taggtgcccg taccgcaaac cgacacaggt
       61 agtcaagatg agaattctaa ggtgagcgag cgaactctcg ttaaggaact cggcaaaatg
      121 accccgtaac ttcgggagaa ggggtgctct ttagggttaa cgcccagaag agccgcagtg
      181 aataggccca agcgactgtt tatcaaaaac acaggtctct gctaaaccgt aaggtgatgt
      241 ataggggctg acgcctgccc ggtgctggaa ggttaagagg agtggttagc ttctgcgaag
      301 ctacgaatcg aagccccagt aaacggcggc cgtaactata acggtcctaa ggtagcgaaa
      361 ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa cgatttgggc actgtctcaa
      421 cgagagactc ggtgaaatca tagtacctgt gaagatgcag gttacccgcg acaggacgga
      481 aagaccccgt ggagctttac tgtagcctga tattgaaatt cggcacagct tgtacaggat
      541 aggtaggagc ctttgaaacg tgagcgctag cttacgtgga ggcgctggtg ggatactacc
      601 ctagctgtgt tggctttcta acccgcacca cttatcgtgg tgggagacag tgtcaggcgg
      661 gcagtttgac tggggcggtc gcctcctaaa aggtaacgga ggcgctcaaa ggttccctca
      721 gaatggttgg aaatcattca tagagtgtaa aggcataagg gagcttgact gcgagaccta
      781 caagtcgagc agggtcgaaa gacggactta gtgatccggt ggttccgcat ggaagggcca
      841 tcgctcaacg gataaaagct accccgggga taacaggctt atctccccca agagttcaca
      901 tcgacgggga ggtttggcac ctcgatgtcg gctcatcgca tcctggggct gtagtcggtc
      961 ccaagggttg ggctgttcgc ccattaaagc ggtacgcgag ctgggttcag aacgtcgtga
     1021 gacagttcgg tccctatccg tcgtgggcgt aggaaatttg agaggagctg tccttagtac
     1081 gagaggaccg ggatggacat acctctggtg taccagttgt cgtgccaacg gcatagctgg
     1141 gtagctatgt gtggacggga taagtgctga aagcatctaa gcatgaagcc cccctcaaga
     1201 tgagatttcc caacttcggt tataagatcc ctcaaagatg atgaggttaa taggttcgag
     1261 gtggaagcat ggtgacatgt ggagctgacg aatactaatc gatcgaagac ttaatcaaaa
     1321 taaatgtttt gcgaagcaaa atcactttta cttac
//
LOCUS       DAMZKC010000308         1351 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.312, whole genome shotgun sequence.
ACCESSION   DAMZKC010000308 DAMZKC010000000
VERSION     DAMZKC010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.312"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            137..1291
                     /locus_tag="POB55_002748"
     CDS             137..1291
                     /locus_tag="POB55_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDI6501647.1"
                     /translation="MKLSLNSNSKYLRAPSIRQFSNRMNNLDDCVNLTIGQPDFPMPD
                     VVKKAYIDAINNDKTSYSHNKGLLETREAISQYFKNRYHFSYDPEEIIVTNGASEAID
                     TTLRSIIEPGDEIIIPGPIYAGYIPLIEVLGGKPIYIDTTATQFKITPDALESHISPK
                     TKAVLLNYPTNPTGVVLKRNEVLNIVNVLKKYPIFIISDEIYAENTFSGKHVSFAEFE
                     DIRDQLILIGGLSKSHSATGIRIGFLLGPQYLIDKLTFMHAYNCICANVPAQIACITA
                     LNEGLEAPKYMNEAYVERRNYLVSELTKLGFEITAQPEGAFYIFPSIKHITDDDFEFC
                     VDLLESTHLAIVPGSSFTEFGKGFVRISYAYEMDVLKEGMKRLAKYLNTK"
BASE COUNT          477 a          202 c          214 g          458 t
ORIGIN      
        1 tacagtgtat ttagacattt ttcgtttttc aaataaagct taagtaattt accctttttt
       61 gctttaaaag tatgttatga tattattaat ttctaaattt ttagacaata tttgatttat
      121 atttgaggtg tcacaaatga aactttcttt aaattctaat tctaaatatt taagagcacc
      181 aagtattcgc caattttcaa atcgtatgaa taatttggat gattgtgtta atttaactat
      241 tggccaacca gacttcccga tgcctgatgt tgtgaaaaag gcatatattg atgctattaa
      301 caatgacaag acatcatact ctcacaataa agggttatta gaaactcgcg aagcaattag
      361 tcaatacttc aaaaatcgtt atcatttttc ctatgatcct gaagaaatta ttgtgacaaa
      421 tggagcaagt gaagcaatag acacaacgtt acgttctatc atagagcctg gagatgaaat
      481 tataataccg ggaccaattt atgcaggcta cataccactc atcgaagtac taggtggtaa
      541 accaatttat attgatacaa cagcaacaca atttaaaatt acacctgatg cattagaaag
      601 tcatatttct ccaaagacaa aagctgtctt gttaaattat ccgactaatc caactggcgt
      661 agttttaaaa agaaatgaag ttttaaatat cgtaaatgta ttaaaaaaat atccgatatt
      721 tattattagc gatgagattt atgctgaaaa tacatttagt ggtaaacatg tatccttcgc
      781 tgaatttgaa gacattcgtg atcaattaat attaattggt ggtttaagta aatcacactc
      841 agcaaccgga ataagaattg gttttctatt aggacctcaa tatttgattg ataaattaac
      901 atttatgcac gcctataatt gtatttgtgc caatgttcca gctcaaatag catgtataac
      961 agcacttaac gaaggattag aagctcctaa atatatgaac gaagcttatg tagaacgaag
     1021 aaattattta gtatctgaat taactaaatt aggttttgag attaccgctc aacctgaagg
     1081 cgcgttttat attttcccaa gtattaaaca tatcaccgat gacgatttcg aattttgtgt
     1141 cgatttactt gaatcaacac atttagcaat cgttccaggc tcatccttta cagaattcgg
     1201 taaaggattt gtaaggattt cctatgctta tgaaatggat gttttaaaag aaggtatgaa
     1261 gagacttgct aaatatttaa atactaaata aaaatgatat gttgaactgg acgtcgtgaa
     1321 ctaaattcat agtacgttca gttttttata t
//
LOCUS       DAMZKC010000309         1343 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.313, whole genome shotgun sequence.
ACCESSION   DAMZKC010000309 DAMZKC010000000
VERSION     DAMZKC010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.313"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(39..356)
                     /locus_tag="POB55_002749"
     CDS             complement(39..356)
                     /locus_tag="POB55_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HDI6501648.1"
                     /translation="MKSMVEMQREVDEYIGQFKTGYFSPLANLARLTEEVGELAREIN
                     HTYGEKKKKDSEEANTIKAELGDNLFVLLCLANSMGIDMTESFNETMEKFNTRDKNRF
                     ERK"
     gene            complement(692..>1343)
                     /locus_tag="POB55_002750"
     CDS             complement(692..>1343)
                     /locus_tag="POB55_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001831045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HDI6501649.1"
                     /translation="LPLWAQHKVKSNYEKYSQVRSTSGKTGREVALEILHANGIYDVE
                     VVKGEGFLTDHYDPKKKVVSLSPANYDRPSVAGTAIAAHEVGHAIQDHQGYWFLRFRA
                     ALVPVANLGSSLSYMIIMLGIILTAIGSAFGSTALWIGAGLMSLAVLFSIVTLPVEFD
                     ASSRAMKQITALNIVNEKEYKHARKVLSAAAMTYVAATAVAVAELVRIILLARSSD"
BASE COUNT          436 a          258 c          181 g          468 t
ORIGIN      
        1 atcttaatca aatccaaata aagcatatat ttttatattc actttctttc gaatcgattt
       61 ttatctcttg tattaaactt ttccatagtt tcattaaagc tctctgtcat atctattccc
      121 attgaattcg ctaaacataa caacacaaat aaattatcac ctaattctgc tttaatcgta
      181 tttgcttcct ctgaatcttt cttctttttt tcaccatagg tatgatttat ttcacgtgca
      241 agttcgccca cttcttcagt caatctagct aagttagcta atggtgaaaa atatcctgtt
      301 ttaaattgtc caatatattc atcaacttca cgttgcattt ctaccattga tttcatttct
      361 acgttctcct tatattgcat ttctaatata gtatatatca atttgaagtc tcatgcatgt
      421 ttagtgcaat ctactgactc tagctaaact agatgacaag ttgtttattt tagaagttta
      481 ataattcagt ttatataaat gtaatgcatt cctaactaaa ttaaatcaat tgaaaatggg
      541 attataactt tatgatacgt actactacaa taaaataata tagtgaataa tctaccatta
      601 gaaaaataag cacaaaaaaa ctagcaacca cacaaaatgt gattagctag ttaataagtg
      661 tctaatttaa gttaattgtt aatctataag attaatcact tgaacgcgca agcaaaatga
      721 tacgtacaag ctctgctaca gcgacagcag ttgctgcaac ataagtcatt gctgctgcag
      781 ataatacttt acgcgcatgc ttgtattctt tttcatttac aatgttcaat gccgtaattt
      841 gtttcatcgc tcttgaactc gcatcaaact caactggtaa cgtaacaatt gagaataata
      901 ccgctaatga cattaaacca gcaccaatcc ataaagcagt tgaaccaaat gcactaccta
      961 tcgctgttaa gataatacct aacatgatga tcatataact taatgaactc cctaggtttg
     1021 caacaggtac taatgctgct ctgaatctta agaaccaata tccttggtga tcttgaatgg
     1081 catgaccaac ttcgtgggct gcaattgcag ttccagcaac tgatggtctg tcatagtttg
     1141 caggagatag tgaaacaact ttctttttag gatcgtaatg atctgttaag aatccttcac
     1201 ctttaacaac ttcgacatca taaataccgt ttgcatgtaa aatttctaat gcaacttcac
     1261 gacccgtttt accactagtt gatctaactt gtgaatattt ctcatagtta gatttaactt
     1321 tgtgttgtgc ccataaagga agc
//
LOCUS       DAMZKC010000310         1298 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.314, whole genome shotgun sequence.
ACCESSION   DAMZKC010000310 DAMZKC010000000
VERSION     DAMZKC010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.314"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            12..758
                     /locus_tag="POB55_002751"
     CDS             12..758
                     /locus_tag="POB55_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDI6501650.1"
                     /translation="MLGIIFLFLVISITAGCGIGKEAEVKKSFEKTLSMYPIKNLEDL
                     YDKEGYRDDQFDKNDKGTWIINSEMVIQPNNEDMVAKGMVLYMNRNTKTTNGYYYVDV
                     TKDEDEGKPHDNEKRYPVKMVDNKIIPTKEIKDEKIKKEIENFKFFVQYGDFKNLKNY
                     KDGDISYNPEVPSYSAKYQLTNDDYNVKQLRKRYDIPTSKAPKLLLKGSGNLKGSSVG
                     YKDIEFTFVEKKEENIYFSDSLDYKKSGDV"
     gene            810..>1298
                     /locus_tag="POB55_002752"
     CDS             810..>1298
                     /locus_tag="POB55_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDI6501651.1"
                     /translation="MIKRVNKLVLGISLLFLVISITAGCGMGKEAEIKKSFEKTLSMY
                     PIKNLEDLYDKEGYRDDQFDKNDKGTWIVNSQMAIQNKGEALKIKGMLLKIDRNTRSA
                     KGFYYTNEIKTEKYEVAQDNQKKYPVKMINNKFISTEEVKEENIKKEIENFKFFAQYS
                     NFK"
BASE COUNT          555 a          135 c          245 g          363 t
ORIGIN      
        1 taaacaaatt agtgttaggc attatttttc tgtttttagt cattagtatc actgctggtt
       61 gtggcatagg taaagaagcg gaagttaaga aaagctttga aaaaacattg agtatgtacc
      121 ctattaaaaa tctagaggat ttatacgata aggaaggcta tcgtgatgat cagtttgata
      181 aaaatgataa aggtacatgg attataaatt ctgaaatggt tattcaacct aataatgaag
      241 atatggtagc taaaggcatg gttctatata tgaatagaaa taccaaaaca acaaatggtt
      301 actactatgt cgatgtgact aaggacgagg atgaaggaaa accgcacgac aatgaaaaaa
      361 gatatccggt taaaatggtc gataataaaa tcattccaac aaaagaaatt aaagatgaaa
      421 aaataaaaaa agaaatcgaa aactttaagt tctttgttca atatggcgac tttaaaaatt
      481 tgaaaaatta taaagacgga gatatttcat ataatccaga ggtgccgagt tattcggcta
      541 aatatcaatt aactaatgat gattataatg taaaacaatt acgcaaaaga tatgatatac
      601 cgacgagtaa agctccaaag ttattgttaa aaggttcagg gaatttaaaa ggctcatcag
      661 ttggatataa agatattgaa tttacgtttg tagagaaaaa agaagaaaat atatacttta
      721 gtgatagctt agattataaa aaaagcggag atgtataatc atggctcaat cagaatatga
      781 aatcaatccc ggaaaaagag agtgatgaaa tgataaaacg tgtaaataaa ttagtgcttg
      841 gtattagtct tctgttttta gtcattagta tcactgctgg ttgtggcatg ggtaaagaag
      901 cggaaataaa gaaaagtttt gaaaaaacat tgagtatgta tccgattaaa aatctagagg
      961 atttatacga taaagaagga tatcgtgatg atcaatttga taaaaatgat aagggaacat
     1021 ggattgtaaa ttctcaaatg gcaattcaaa ataaaggaga agctctaaaa ataaaaggca
     1081 tgcttttgaa gatagataga aatacaagaa gtgcaaaagg attttactat actaatgaaa
     1141 taaagacgga gaaatacgaa gtagctcagg ataatcaaaa aaaatatcca gttaaaatga
     1201 ttaataataa attcatttct actgaggaag ttaaagaaga aaacataaaa aaagaaatcg
     1261 aaaactttaa gttttttgcg caatatagca attttaaa
//
LOCUS       DAMZKC010000311         1296 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.315, whole genome shotgun sequence.
ACCESSION   DAMZKC010000311 DAMZKC010000000
VERSION     DAMZKC010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.315"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(164..1039)
                     /locus_tag="POB55_002753"
                     /pseudo
     CDS             complement(164..1039)
                     /locus_tag="POB55_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365429.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
BASE COUNT          471 a          225 c          161 g          439 t
ORIGIN      
        1 aaaaatactt attttaagta gatgagcata agtgaacatg tccccatttt tttgcaaaat
       61 tttgcaaaaa atgagtggtt tggcggtgcc aaacatgttc aaaacgtggg gacaaaaaag
      121 catgattgca cataaattga acatagttct ttagttataa taattaattc aaccaaaagt
      181 cgatttgttt ttgcaattgg ttttcatttc ctcttaaaga tattttcatt aaatctgtca
      241 aatcaataga cgctatattt ttcaacttat ctctatattt atttttagta cgtctttcta
      301 aatttcccca ttcctcttct tcgtgagtta ataaatgaag cattgctcgt tcttgtatat
      361 tttcaatcat ttttaaattc ggttttaaaa tatgcaaatc atcaaaacaa tctttccaac
      421 aatcaaccat atctcgtttt aattcaattt ccacacgcca tagaaatgtt gaatcaattt
      481 caacatctgc attatcttta cgttcttgtt tttattataa atccgaataa acctatcact
      541 attacgcaca ccaaaatatt ttgtttctgg ttttacatta cgtccataaa atatagtttt
      601 ctttgccgac ttatctgaca atgcataata gtcatttaaa tcaaattcaa aatcaaaagc
      661 caaatctaat ctcgtaaaac taacatcgtc caaataactg atgatatttt gttttaacca
      721 aagcacttca tcatgcgaaa gcttattagg attaaattca acgcgcatac tacgtctatt
      781 ccaaagagtt gcttttattt tgtcatattc aatataaact ttttctttaa gagctttagc
      841 tttaaagttt gtttgtaaaa tatcccaaag ccgaacttca ggattagtac tcataaaatg
      901 tgaaagtctc tctgcgttag acatgctaag attcccaaca atcgttatag cgtcaaaaga
      961 caattttgga atagctagtg acatcctatg tcaatttaac cggctattac cggatattag
     1021 agtatccagt tttacacatg gatgaaacga aattcaaaac cctaaaaaat atgttccact
     1081 aacagcaaaa aaataccatt atgttcctac taaaaaacta aaaatactgg agaacaaatg
     1141 tcaggatata acttaggata ctatgtaata aaaatttaca ataaaaaaac aggaaaacaa
     1201 atttcaagta aaagaatacc catacaaaga ggataaaata aaaaacctcg aactgaaatg
     1261 atgatctttt cagctcgagg tttaaatatt agtgcc
//
LOCUS       DAMZKC010000312         1275 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.316, whole genome shotgun sequence.
ACCESSION   DAMZKC010000312 DAMZKC010000000
VERSION     DAMZKC010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.316"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(177..1202)
                     /locus_tag="POB55_002754"
     CDS             complement(177..1202)
                     /locus_tag="POB55_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HDI6501652.1"
                     /translation="MISFENDYLEGAHEKVLKRLVDTNLVQASGYGFDQFTAQAIEKI
                     KDTIDCPNATIRFLVGGTQTNQVVINSMLESYEGVISADTGHVAVHEGGAIEYSGHKV
                     ITIPSKEGKVSASDVETYMETFKSDFKKDHMVFPGMVYISHPTEYGTLYSKSELEELC
                     KVCKQYQLPLFMDGARLGYGLMSDQSDMTIKDIAKYCDVFYIGGTKIGALCGEAIVFT
                     KNNEPKQFTTRIKHHGALLAKGRLTGIQFLELFTDNLYFNISRHAIEMANKMKDGFIN
                     KGYRLYFDSPTNQQFFILSNEKIAELEQKVKFAVWEKYDDQHRVVRFATSWATTEENL
                     NKLLELI"
BASE COUNT          416 a          241 c          166 g          452 t
ORIGIN      
        1 tatataaaat aaaacaagat actcaataat ctgtagtatc ttgttttgga atgatgtaat
       61 tttataacat caacggcttc aaatgagtag tttcatatat aaatcaataa acacaactac
      121 tcaactgaac tggcttaatt aataaatgat ttgccagacg aaatataaca gcattattag
      181 attaattcta atagtttatt taaattttct tcggttgtcg cccagctggt tgcgaatcta
      241 acaacacgat gttgatcatc gtatttttcc caaacagcaa atttaacttt ttgttctaac
      301 tctgctattt tctcgttact taaaataaaa aattgttgat tggttggaga atcaaagtaa
      361 agacgatagc ctttatttat aaacccgtct ttcatcttat ttgccatttc gatagcatgt
      421 ctgcttatat taaaatataa attatccgta aataattcta aaaattgtat gcctgttaac
      481 cgtccttttg ctaaaagtgc accgtgatgt ttgattcgag tggtaaattg tttcggttca
      541 ttattcttcg taaaaacaat tgcttctccg cataatgcac ctatcttcgt accacctata
      601 taaaatacat cacaatattt agcgatgtct ttaatagtca tatctgattg atcactcatc
      661 aatccatacc ctaatcgtgc accatccata aataatggaa gctgatattg cttacatact
      721 ttgcataact cttccaattc tgatttagag tatagtgtgc catattctgt aggatgagaa
      781 atatatacca ttcctgggaa taccatatgg tcctttttaa aatcactttt aaatgtctcc
      841 atgtaagttt caacatctga agcactaact tttccttcct tagagggtat agtaattact
      901 ttatgtccac tatattcaat tgcaccgccc tcatgcacag caacatgacc agtgtctgct
      961 gaaataaccc cttcgtaact ttctaacatt gaattaataa caacctgatt ggtttgcgtt
     1021 ccacctacta aaaaacggat agtagcattt ggacaatcaa ttgtatcttt aatcttttca
     1081 attgcctgag ctgtgaattg atcaaagcca tatcccgaag cttgtacaag atttgtatct
     1141 actaatcgtt ttaatacttt ttcatgagca ccttctaaat aatcattttc aaatgagatc
     1201 actacatttc cccctaaaac taatatcagc attttaataa gataaactaa tttcaaaact
     1261 agttcgatat ttaaa
//
LOCUS       DAMZKC010000313         1234 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.318, whole genome shotgun sequence.
ACCESSION   DAMZKC010000313 DAMZKC010000000
VERSION     DAMZKC010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1234
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.318"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            28..1146
                     /locus_tag="POB55_002755"
     CDS             28..1146
                     /locus_tag="POB55_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDI6501653.1"
                     /translation="MNKQQSKVRYSIRKVSIGILSISIGMFLALGMSNKAYADEIDKS
                     KDFTRGYEQNVFAKSELNANKNTTKDKIKNEGAVKTSDTSLKLDNKSAISNGNEINQD
                     IKISNTPKNSSQGNNLVINNNEPTKEIKIANLEAQNSNQKKTNKVTNNYFGYYSFREA
                     PKTQIYTVKKGDTLSAIALKYKTTVSNIQNTNNIANPNLIFIGQKLKVPMTPLVEPKP
                     KTVSSNNKSNSNSSTLNYLKTLENRGWDFDGSYGWQCFDLVNVYWNHLYGHGLKGYGA
                     KDIPYANNFNSEAKIYHNTPTFKAEPGDLVVFSGRFGGGYGHTAIVLNGDYDGKLMKF
                     QSLDQNWNNGGWRKAEVAHKVVHNYENDMIFIRPFKKA"
BASE COUNT          521 a          153 c          208 g          352 t
ORIGIN      
        1 gtatattatt gtaaggagtg tttcatcatg aataaacaac aaagtaaagt acgctattca
       61 attagaaaag ttagtattgg aattttgtca atttcaatag gtatgttttt ggcattgggt
      121 atgtcgaaca aagcatatgc agatgaaatt gataaatcta aagattttac aagagggtat
      181 gagcaaaatg tattcgcgaa atcagagtta aatgctaata aaaatacgac aaaagacaaa
      241 ataaaaaatg aaggtgctgt taaaacatcg gacacaagtt taaagttaga caacaaatca
      301 gcaatttcaa acggaaatga aattaatcaa gatataaaga tttcaaatac tccgaaaaac
      361 tcaagccaag gtaacaatct agttattaat aacaatgaac ctactaaaga aattaaaatt
      421 gcaaacttgg aagctcaaaa ttctaatcag aagaaaacga ataaagttac taataattac
      481 tttggttact acagttttag agaagctcca aaaacacaaa tctatactgt aaaaaaagga
      541 gacacactta gtgctatagc attaaaatac aaaactacag tttcaaatat tcaaaataca
      601 aataatatag caaatcctaa tttaatattt attggtcaaa aattaaaagt gccaatgaca
      661 ccattagtag aaccaaaacc aaaaacagtg tcttcaaata ataaaagtaa tagtaatagc
      721 agtacattaa attatttgaa aacattagag aatagaggat gggatttcga cggtagttat
      781 ggatggcaat gtttcgattt agttaatgta tattggaatc atctttatgg tcatggatta
      841 aaaggatatg gagctaaaga tataccatat gcaaataatt ttaatagtga agctaaaatt
      901 tatcacaaca caccaacttt caaagctgaa cctggggact tagtggtttt tagtggaaga
      961 tttggtggag gatatggtca tacagctatt gtcttaaatg gtgattatga tggaaaatta
     1021 atgaagttcc aaagtttaga tcaaaactgg aataatggtg gatggcgtaa agcagaggtt
     1081 gcacataaag ttgttcataa ttatgaaaat gatatgattt ttattagacc atttaaaaaa
     1141 gcataattta aatcgaaggc aggacattga aatatgaaat tttcaacttt aagtgaagaa
     1201 gaatttacca actacaccaa aaagcacttc aaac
//
LOCUS       DAMZKC010000314         1232 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.319, whole genome shotgun sequence.
ACCESSION   DAMZKC010000314 DAMZKC010000000
VERSION     DAMZKC010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1232
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.319"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(225..1118)
                     /locus_tag="POB55_002756"
     CDS             complement(225..1118)
                     /locus_tag="POB55_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDI6501654.1"
                     /translation="MKKTILLTMTTLTLFSMSPNSAQAYTNDSKTLEEAKKAHPNAQF
                     KVNKDTGAYTYTYDKNNTPNNNHQNQSRTNDNHQHANQRDLNNNQYHSSLSGQYTHIN
                     DAIDSHTPPQTSPSNPLTPAIPNVEDNDDELNNAFSKDNKGLITGIDLDELYDELQIA
                     EFNDKAKTADGKPLALGNGKIIDQPLITSKNNLYTAGQCTWYVFDKRAKDGHTISTFW
                     GDAKNWAGQASSNGFKVDRHPTRGSILQTVNGPFGHVAYVEKVNIDGSILISEMNWIG
                     EYIVSSRTISASEVSSYNYIH"
BASE COUNT          353 a          182 c          227 g          470 t
ORIGIN      
        1 atgtttaata agttattgga aatctaataa actacaaaaa atattttgat tacataatga
       61 ttatttaaaa atgtcggtta actttaaggt gacaagttat tgcaattttg aaggtagaca
      121 tcatatttta taatcgaata acatacaaaa taaagcgacc aattttgcag tgttacgaat
      181 tgtaaactgc gaactggtcg ctttttattg atgtcatgat taatttaatg gatgtaatta
      241 tatgatgaaa cttctgaagc agagatggtt cttgatgaaa cgatatattc accaatccag
      301 ttcatttctg aaattagaat acttccatca atattaactt tttcaacgta ggctacatga
      361 ccaaatggac catttactgt ttgtaaaatt gatcctcgtg ttgggtgtct atctactttg
      421 aagccattgc ttgaagcttg gcctgcccag tttttagcat ctccccaaaa tgtactaatc
      481 gtgtgtccat ctttggcacg tttatcaaag acataccatg tacattgtcc agcagtatat
      541 aagttgttct tacttgtgat aagaggctga tcaatgattt taccgttacc taatgctaaa
      601 ggtttaccgt cagcggtctt tgctttgtca ttaaattcgg cgatttgtaa ttcgtcatac
      661 aattcgtcta aatcgatgcc tgtaataagc cctttgttat cttttgaaaa agcgttattt
      721 aattcatcgt cattgtcttc gacattcggt attgctggtg tcaaaggatt gcttggtgac
      781 gtttgaggcg gtgtgtgtga atcaattgcg tcattaatgt gcgtatactg accacttaat
      841 gaagaatggt actgattgtt gttaagatca cgttgatttg cgtgttgatg attgtcgttt
      901 gtacgtgact ggttttgatg attgttgttt ggcgtgttgt ttttgtcata tgtataagta
      961 tacgcgccgg tgtctttatt cactttgaac tgtgcgtttg ggtgtgcttt ctttgcttct
     1021 tctaatgttt tgctatcatt cgtatatgct tgagccgagt taggcgacat actaaataaa
     1081 gtaagagttg tcatcgtcag taaaattgtt ttcttcataa taaccattta atcctttatg
     1141 tatttaattt aattttagta tacacattta tattacaaaa atgaatggtt aattaaaaat
     1201 atatgggtat attcaatata tttatttaaa aa
//
LOCUS       DAMZKC010000315         1231 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.320, whole genome shotgun sequence.
ACCESSION   DAMZKC010000315 DAMZKC010000000
VERSION     DAMZKC010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.320"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            78..338
                     /locus_tag="POB55_002757"
     CDS             78..338
                     /locus_tag="POB55_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501655.1"
                     /translation="MNNILLNAINIVITTTFVIFNILITYNKDLDDLCWLLPGIIICG
                     VILIVSFTIAMITKNWLSEILFFINIVLVLYYIYPIFYSFIG"
     gene            367..555
                     /locus_tag="POB55_002758"
     CDS             367..555
                     /locus_tag="POB55_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501656.1"
                     /translation="MSLHFAILFWLALIFLVAATFILVLMKKTGKESKKESYLSFTVI
                     LYIFGFAILIYTFIFGVL"
     gene            743..>1231
                     /locus_tag="POB55_002759"
     CDS             743..>1231
                     /locus_tag="POB55_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501657.1"
                     /translation="MNYIKRTIILLILFVVVSPINSPKTIADNKYSEIQDDKFQLQPG
                     DIIVTKGPVMWGFFGHCSIAIDDKTILQIEGPGDKPTTQSFESFKYNYASGKNDWMKV
                     YRCSYPGAGKKAAEWVKKNYENTNHRYLVTLNLNSKKFTYCTKIIYQAYKFGVSEKSV
                     KSY"
BASE COUNT          412 a          173 c          177 g          469 t
ORIGIN      
        1 tactttttat ttacctattg gaaaatagtg ttatcatatt tattaagaag ctatttttat
       61 actagtaggt gtcagtcatg aataacattt tgttaaatgc tatcaatata gttattacta
      121 ccacttttgt tatctttaat attttaatca catataataa agatttagat gatttatgtt
      181 ggctcctgcc tggtattatc atttgtggtg tgatactcat cgtatccttt accattgcaa
      241 tgataactaa aaactggtta agtgaaatat tattttttat aaatatcgta ctcgttctct
      301 attacattta tcctattttt tatagtttta taggttaata attacggtat ataaggaggg
      361 tttcacatgt ctttacattt tgcaattctg ttttggctag cattaatttt cttagttgcc
      421 gctacgttta tactcgtatt aatgaaaaaa actggcaaag aatctaaaaa agagtcctat
      481 ttaagtttca ctgtcattct ctatattttt ggattcgcta tattaatata cacatttata
      541 tttggtgtgc tataaatgaa attcatttta tatccagtct gaaacgtctc aatgatgttt
      601 cagactgttt tttattctta atagacattt cacactctca tactttaata tttaagatat
      661 gttttagtct tacaagctta ttaaagatta caattctaat ttaagactat ttacatatat
      721 ttcttcaaca aggagctttt aagtgaacta tattaaacga acgataattt tacttatatt
      781 attcgttgtc gtatccccaa taaactcacc aaaaacaatt gcagataata agtattcaga
      841 aattcaagat gacaaattcc aattacaacc tggtgatatt attgttacaa aaggccctgt
      901 catgtgggga ttttttggtc attgtagtat cgcgattgat gataaaacga ttttacaaat
      961 tgaggggcct ggcgacaaac caactacaca atccttcgaa tcttttaaat ataattatgc
     1021 aagtggcaaa aatgattgga tgaaagttta tcgttgcagc tatcctggtg caggtaaaaa
     1081 agcggctgaa tgggttaaga aaaactatga gaatacaaat catcgttatc tagttacatt
     1141 aaatttgaac agcaaaaaat ttacgtattg tactaaaatt atctaccaag cttataaatt
     1201 tggtgtaagt gaaaaatcag ttaaaagtta t
//
LOCUS       DAMZKC010000316         1208 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.321, whole genome shotgun sequence.
ACCESSION   DAMZKC010000316 DAMZKC010000000
VERSION     DAMZKC010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.321"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..75
                     /locus_tag="POB55_002760"
     CDS             <1..75
                     /locus_tag="POB55_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3885 domain-containing protein"
                     /protein_id="HDI6501658.1"
                     /translation="NNEDYARYSEKYNDLISEFDNEDI"
     gene            complement(168..329)
                     /locus_tag="POB55_002761"
     CDS             complement(168..329)
                     /locus_tag="POB55_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501659.1"
                     /translation="MLLILELRFWNSEFGFRNSTFGIQNYNEWKILIIACLMTWFCSD
                     FYLMNCFFV"
     gene            complement(366..1106)
                     /locus_tag="POB55_002762"
     CDS             complement(366..1106)
                     /locus_tag="POB55_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HDI6501660.1"
                     /translation="MFILRNDLETLKHIIDSSNRITFFTGAGVSVASGVPDFRSMGGL
                     FDEISKDGLSPEYLLSRDYLEDDPEGFINFCHKRLLFVDTKPNIVHDWIAKLERNQQS
                     LGVITQNIDGLHSDAGSQHVDELHGTLNRFYCNACHKSYTKSDVIDRTLKHCDNCGGA
                     IRPDIVLYGEMLDQPTIIRSLNKIEHADTLVVLGSSLVVQPAAGLISNFKGDNLIIIN
                     KDRTPYDRDATLVIHDDMVSVVKSLMTE"
BASE COUNT          430 a          222 c          164 g          392 t
ORIGIN      
        1 aataatgaag attatgcaag atattctgaa aaatataatg atttaatatc ggaatttgat
       61 aatgaagata tctaattttt taatattcgt agcttttatt tttctactta ttttatgttt
      121 atttttaatc ttacaaatga caaaccatta aaagtagcat cccaacatca aacaaaaaaa
      181 caattcatca aataaaaatc gctacaaaac caagtcatta aacacgcaat aattaaaatt
      241 ttccactcat tataattctg aattccaaat gtcgaattcc gaaaaccaaa ctccgaattc
      301 caaaaacgca actccaaaat taaaagcatc tccctgttac tcgggaaatg ctttttacct
      361 actgattact ctgtcattaa tgattttaca acggaaacca tgtcgtcatg tatgaccaaa
      421 gtagcgtctc tatcataagg tgttcgatct ttattgataa taattaaatt gtcgccttta
      481 aaatttgata ttaatcctgc ggcaggttgt acaacgagtg atgaacctag tacaacaagg
      541 gtgtcagcat gttcaatttt atttaatgac cgtatgatgg taggttgatc taacatttca
      601 ccgtataata cgatgtccgg tcgaatggca ccaccacaat tatcacaatg tttcaaagtt
      661 ctatcaatga catctgactt cgtataagat ttatgacatg cattacaata aaaacgattt
      721 aacgtgccat gtaattcatc aacatgttga cttccagcgt ctgagtgcaa accatcgata
      781 ttttgcgtga tgacacctaa agattgttga ttacgttcta attttgcaat ccaatcatga
      841 acgatattag gcttcgtatc gacaaatagt aaacgcttat ggcagaaatt gataaaacct
      901 tcaggatcat cttctaaata atcacggctt aacaagtatt ctggcgaaag tccatctttt
      961 gaaatttcat caaataagcc acccattgaa cggaaatctg gaacgccact tgcgacagat
     1021 acaccagcac ctgtgaaaaa tgtaatacga ttcgaactat ctataatatg ttttaatgtc
     1081 tctaaatcat ttctcaaaat aaacacccca tcaaaataat gaatgattta taacgtcttt
     1141 tgcttttatt atagtatatt cattttacga ttatcaaaag cgcagtttaa acagttcttt
     1201 ttaaaaat
//
LOCUS       DAMZKC010000317         1185 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.322, whole genome shotgun sequence.
ACCESSION   DAMZKC010000317 DAMZKC010000000
VERSION     DAMZKC010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.322"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            43..>1185
                     /locus_tag="POB55_002763"
     CDS             43..>1185
                     /locus_tag="POB55_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HDI6501661.1"
                     /translation="MGYRTLKSIFHEHNESKMKEEYTKRFNSLASFNTNINIIPMENG
                     KKVNDLEYPLFFMVTKNLSKKQELISINSRKIDRALNSLPYAAREQYFNDLLIDELQS
                     TNEIENVFSTKQEIAHALNNQASEFLKFRGLVDQYKEIELNKKIKVDNVRDIRAIYDK
                     LVSNEINEQDKLDGELFRKNFVGVHDGSTNKYIHVGLQPETKIVEYIGEMLTFLKYFD
                     APQPFKIMASHYLFEYIHPFYDGNGRVGRFIIAKLLRDYYDNYTALTFSYVINRNKSK
                     YYKAFMTASNHLNCGDLTEFIDTMLELLIAGQERILDELIPKMDATEKLTLYLTSHYK
                     QIDYEFLYLLSMDKLFGNKRNRLTLIDLENILGVGRVKINNTIKKYD"
BASE COUNT          477 a          127 c          198 g          383 t
ORIGIN      
        1 ttggacaaaa aatacatttt aaatgagtag aggaggctgc aaatgggtta cagaacttta
       61 aaaagtattt ttcatgaaca caatgaaagt aaaatgaagg aagagtatac taaaagattt
      121 aattctttag cttctttcaa tactaatatt aatatcatac ctatggaaaa tggaaaaaaa
      181 gttaatgatt tggaatatcc tctattcttt atggtgacta aaaatctatc aaaaaaacaa
      241 gaattaatat caattaatag tagaaaaatt gatagagcac ttaattcttt accatatgca
      301 gctagagaac aatattttaa tgatttatta atcgatgaat tacaaagtac taatgaaatt
      361 gaaaatgtat ttagtactaa acaagagatt gcacatgcgt taaataacca agcatcagaa
      421 tttcttaagt tcagaggcct cgtggatcaa tataaagaga tagaacttaa taaaaaaatt
      481 aaagttgata atgtaagaga cattagagcg atttatgata aattagtttc aaatgaaatt
      541 aacgaacaag ataagttaga tggagagcta tttcgtaaaa attttgtcgg tgtgcatgat
      601 gggtcaacga ataaatatat acatgttggg ttacaacctg aaaccaaaat tgttgaatat
      661 ataggtgaaa tgctaacatt tttaaaatat tttgatgcgc ctcagccgtt caaaatcatg
      721 gctagtcatt atctgtttga atatatacat cctttttatg atggcaatgg tagagttgga
      781 agatttataa ttgccaagct cttgagagat tattatgata attatactgc tttgacattt
      841 tcatatgtta taaatagaaa taaatcgaaa tattacaagg cgtttatgac tgcttcaaat
      901 catttgaatt gtggtgattt gacggaattt attgacacaa tgcttgagct attgatagca
      961 ggacaagaaa gaatacttga tgagttaata ccaaaaatgg atgcgactga aaaattaacg
     1021 ctttatttaa ctagtcatta taaacagata gattatgaat ttttgtatct cctttcaatg
     1081 gataaattgt ttggaaataa aagaaataga ttaacattaa ttgatttaga aaacatattg
     1141 ggagtaggaa gagtaaaaat taataataca attaaaaaat atgat
//
LOCUS       DAMZKC010000318         1176 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.323, whole genome shotgun sequence.
ACCESSION   DAMZKC010000318 DAMZKC010000000
VERSION     DAMZKC010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.323"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            667..852
                     /locus_tag="POB55_002764"
     CDS             667..852
                     /locus_tag="POB55_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI6501662.1"
                     /translation="MLNLKELREEKGITRYQLAKLTELQNSTIRSIETEVKNPGFLTV
                     KKICDALQVDIANVKEK"
     gene            854..>1176
                     /locus_tag="POB55_002765"
     CDS             854..>1176
                     /locus_tag="POB55_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HDI6501663.1"
                     /translation="MQALQTKSNIGEMFNIQEKENGEIAISGRELHQALEVKTRYNDW
                     FERMINYGFEENIDYTALTQKRVTAQGNAINYLDHALTLDTAKEIAMIQRSEPGKRAR
                     QYFIQV"
BASE COUNT          460 a          199 c          205 g          312 t
ORIGIN      
        1 tttatctaaa aatattaatt cttctaaagt atgagttcta gacgagtcat tagcgacgac
       61 aaaatttaat tctacaaaat tattttcata gtagaaatta agatcgctaa taacaaaact
      121 gaacttattt tgagaacgag tataaaaaat tgagtatgca gatagaccta aagctagaaa
      181 agctacaacg tttgaaaaca aagtagattg tatgaattcc ataagaataa ccaccttaaa
      241 tatttgataa caacattata cacgaaagga gcataaatat tatgcaagca ttacaaacat
      301 gttgtttcca ataaaaaaca cacaccttgt cgtagaaggt atgtgttacg gaaattttgt
      361 tcggttctaa ttactacgac taacagcaca atttttgctg gtatcgtccc cagccctgta
      421 tggtgcttag gttttccgtc aaagtctagc gtcctaaaag ttactacctt ctagtacgca
      481 taccctagtt aacgtctctt ggttgactgt ggaacacaac aaacgatgtt ctaatttaga
      541 cttactaacc tataaaacca caggatgatt taaaacctcg cataagcaag gaaatcacct
      601 cccaatgtag tggggttgga ttaattatat aacgaaatat cgttatggac aataaggagt
      661 ggtaagatgc tgaacttaaa agaattgaga gaagaaaagg ggataacacg ctatcaacta
      721 gcgaagctaa cggaattaca aaactcgaca attcgatcta tcgaaacaga ggttaaaaac
      781 cccggtttcc tcacagtaaa aaaaatatgc gatgcactac aagttgatat cgctaatgta
      841 aaggagaaat agaatgcaag cattacaaac aaaatcgaac atcggagaaa tgttcaatat
      901 tcaagaaaaa gaaaatggag aaatcgcaat aagtggtcga gaacttcatc aagcattaga
      961 agttaagaca agatataacg attggtttga aagaatgatt aattatggct ttgaagaaaa
     1021 tattgattat acagctctta ctcaaaaaag agtaacagct caaggtaacg ctattaatta
     1081 tttagaccac gcactcacac tagacactgc aaaagagatt gcaatgattc aacgcagtga
     1141 acccggtaaa cgtgcaagac aatatttcat ccaagt
//
LOCUS       DAMZKC010000319         1169 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.324, whole genome shotgun sequence.
ACCESSION   DAMZKC010000319 DAMZKC010000000
VERSION     DAMZKC010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.324"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            52..>1169
                     /gene="ald"
                     /locus_tag="POB55_002766"
     CDS             52..>1169
                     /gene="ald"
                     /locus_tag="POB55_002766"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HDI6501664.1"
                     /translation="MLVAVVKELKQGEGRVACTPENVRKLTDAGHKVIVEKNAGIGSG
                     FSNDMYEKEGAKIVTHEQAWEADLVIKVKEPHESEYQYFKKNQIIWGFLHLASSKEIV
                     EKMQEVGVTAISGETIIKNGKAELLAPMSAIAGQRSAIMGAYYSEAQHGGQGTLVTGV
                     HENVDIPGSTYVIFGGGVAATNAANVALGLNAKVIIIELNDDRIKYLQDMYAEKDVTV
                     VKSTPENLAEQIKKADVFISTILIPGAKPPKLVTREMVKSMKKGSVLIDIAIDQGGTI
                     ETIRPTTISDPVYEEEGVIHYGVPNQPGAVPRTSTMALAQGNIDYILEICDKGLEQAI
                     KDNEALSTGVNIYQGQVTNQGLASSHDLDYKEILNVIE"
BASE COUNT          455 a          164 c          241 g          309 t
ORIGIN      
        1 aaggataaaa taattgtgaa attattcaca aatgaaagag gagtgttgca aatgttagtt
       61 gcagtagtca aagaattaaa acaaggtgaa ggacgtgtag cttgcacacc cgaaaatgtg
      121 cgtaagttaa cggatgcagg acataaagta attgttgaaa aaaatgctgg cattggttca
      181 ggattttcta acgatatgta tgaaaaagaa ggcgctaaga tcgtaactca tgaacaagca
      241 tgggaagctg atcttgttat caaagtaaaa gaacctcatg aaagcgaata tcaatatttc
      301 aaaaagaatc aaattatctg gggattttta catttagcat cttcaaaaga aatagtagaa
      361 aaaatgcaag aagttggtgt aactgcgatt agtggtgaaa ccattataaa aaatggaaaa
      421 gcagaattat tagcgccaat gagtgctata gcaggtcaac gctcagcaat tatgggagct
      481 tactactctg aagcacaaca tggtggtcaa ggtactttag tgactggtgt acatgaaaat
      541 gtggatatac ctggtagtac atatgtgatt ttcggtggtg gagtagcagc aacaaatgca
      601 gcaaatgttg ccttgggact aaatgctaaa gtaatcatta tcgagttaaa cgatgatcgc
      661 attaaatatt tacaagatat gtatgcagaa aaagatgtca cagtagtcaa atcaacacca
      721 gaaaatttag cagaacaaat aaaaaaagca gatgtattta tttctacaat tttaattcct
      781 ggtgcgaaac cgccaaaatt ggttactcgt gagatggtta aatcaatgaa aaaaggttca
      841 gtattaatcg atatagctat tgaccaaggt ggaactattg aaacaattag accaactaca
      901 atttctgatc cagtgtatga agaagaaggt gtgattcatt atggtgtacc aaaccaacca
      961 ggagcagtcc caagaacttc aacaatggca ttagcacaag gaaatattga ttatatatta
     1021 gaaatttgtg acaaaggctt agaacaagca attaaagata atgaagcctt aagtactggt
     1081 gtaaacattt accaaggaca agtgacaaat caaggattag cttcatcaca tgacctagat
     1141 tataaagaaa tattaaatgt tatcgaata
//
LOCUS       DAMZKC010000320         1153 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.325, whole genome shotgun sequence.
ACCESSION   DAMZKC010000320 DAMZKC010000000
VERSION     DAMZKC010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.325"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(<1..958)
                     /locus_tag="POB55_002767"
     CDS             complement(<1..958)
                     /locus_tag="POB55_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HDI6501665.1"
                     /translation="MEEFKQHYKGLIDESLTCQDKVELIKKCEKYTDEVIRKDVLPED
                     IVDIHKNYILTLNLTREDVFKTLDVLQEIVKGFGYSYRDYQRLVDKLQVHDKEIDLAS
                     SLQQTMLKTDIPQFDSIQIGVISVAAQKVSGDYFNLIDHNDGTMSFAVADVIGKGIPA
                     ALAMSMIKFGMDSYGHSQLPSDGLKRLNRVVEKNINQNMFVTMFYGLYEEMNHLLYCS
                     SAGHEPGYIYRAEKEEFEEISVRGRVLGISSQTRYQQQEIPIYLDDLIIILTDGVTEA
                     RNSEGTFIDKQKLLEYIKKHKHMHPQDIVQIIYEAILKLQNPN"
BASE COUNT          359 a          215 c          151 g          428 t
ORIGIN      
        1 tatttgggtt ttgaagcttt aaaattgctt catagataat ttgaacaata tcttgtgggt
       61 gcatatgttt atgtttttta atatattcta aaagtttttg tttatctata aaggtacctt
      121 cactatttct agcttcagtc acaccatccg ttaaaatgat aattaaatca tcaaggtata
      181 ttggaatttc ttgttgttga tatcgtgttt gtgaactgat tcctaacact ctacctctaa
      241 ctgaaatttc ttcgaattct tctttttcag cgcgataaat atatccaggc tcatgaccag
      301 ctgaactaca atacaataaa tggttcattt cttcatataa accataaaac attgtgacga
      361 acatattttg attaatattc ttttcaacaa cacgatttaa acgttttaaa ccatcactcg
      421 gtaattgtga gtgtccataa gaatccatgc caaactttat catactcatt gctaaagcag
      481 ctggtatacc ttttccaatg acatctgcaa cagcaaagct cattgtgcca tcgttatggt
      541 caattaaatt aaaataatct ccacttactt tttgtgccgc cactgaaata acgccaattt
      601 gaatactatc aaattgtgga atatctgttt taagcattgt ttgttgtaag ctagaagcta
      661 agtctatctc tttatcgtga acttgaagtt tatctaccaa tctttgataa tctcgataac
      721 tataaccaaa gcctttaacg atttcttgta agacatctaa tgtcttgaac acatcttcac
      781 gcgttaagtt taacgtcaat atatagtttt tgtgaatatc gacaatgtct tcaggcaaga
      841 cgtccttacg aatcacttcg tcagtgtatt tctcacactt ttttatcaat tctactttat
      901 cttggcacgt taaactttca tcaattaaac ccttataatg ttgcttaaat tcttccacga
      961 ttagttgcct cctcacttcc cttacaaagc aaatgcttaa gcaaggataa ttatgattca
     1021 cttactaaca tttatttaat tgcacttatc aattttgtcg tttgcaattt tgctgtagat
     1081 gatactttta agctaaaaaa actttaggat ggaataccta aagcctaaaa aacttaagac
     1141 atccttttat aat
//
LOCUS       DAMZKC010000321         1144 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.326, whole genome shotgun sequence.
ACCESSION   DAMZKC010000321 DAMZKC010000000
VERSION     DAMZKC010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.326"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(154..1139)
                     /locus_tag="POB55_002768"
                     /pseudo
     CDS             complement(154..1139)
                     /locus_tag="POB55_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498656.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol-specific phospholipase C"
BASE COUNT          357 a          227 c          131 g          429 t
ORIGIN      
        1 ctttttcact ttaatattat caaaaatttc acaaacaatc tgttcaattt ataatattgt
       61 tgaattatta cttattttca aaataaaaac gagtagtgaa aataattaga atctaactat
      121 tttcatactc gtcttaatat cattattgaa tccttattta ttactatcta taatttcact
      181 tacgatatca tcatatccag gccacgtata tcctgcatag tcaacaatca gccaacccgt
      241 tctagctttc ccatttgctt taatcgtttt tgcaatttca ggatttatat gtgatgcata
      301 gttataagta ctattaaatg cgctgcctcc agacgctaca ctcaagaaat tcaaatatac
      361 attgtctttg ttactatccg ttttagcttt agccaataaa tttttaacag cttcaacttt
      421 tttatcatag taatctttat actcatcttg tacttttaaa tttaagctac cattattaat
      481 tttcgtttca aatgtcgcat tgtctgccca ttgaatacct gacgtgtcag caccataacc
      541 actttttatg tacgtacccc ccattctatt gaataggaca attttacctt tcgtttcttt
      601 taaagtagga ttcgcattac ttcctgtgta aaaaagattc tgatattgcg ggttattata
      661 ataatattct ctaaaaattt cttcaaatgt cttcgtaact ttagaatcgc tatcgtagtc
      721 ctttttcata gacatcacaa ttgtttcttt ggataagcac tcaagtaata tttagcatca
      781 tcgagaaatt ttcctaattc atgatgcaaa taaaccatgc cgtgatgaac tgaaatcata
      841 ttatcagcac ttgctctacc tctaatatca aaaaacctga ctcccgactt catttgggta
      901 aggtaatctt tatcttgagt ctttgcccaa actgatttta ctggatcctt taaagtgaat
      961 gagccactat catgtgaacc cggtatatta atctcagtta aatgttttcc atcatcaagt
     1021 ttactcatcc aattttctgg acttttactc aacgaatctg acgcatgtgc tgaatgatac
     1081 caaccactca tcactactaa aatgatactt aaaaacaaag tcttaataca ctttttcata
     1141 atat
//
LOCUS       DAMZKC010000322         1142 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.327, whole genome shotgun sequence.
ACCESSION   DAMZKC010000322 DAMZKC010000000
VERSION     DAMZKC010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.327"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(<1..1000)
                     /locus_tag="POB55_002769"
     CDS             complement(<1..1000)
                     /locus_tag="POB55_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HDI6501666.1"
                     /translation="MSNANNADNKQFFSKILNSIGAGVVIALVPNALLGEVLKIFKSG
                     NEILELTYQLVILIQSFMAFIIGVLAAHQFKFNGAGAAIVGTSAMIGSGAVVYSNNSF
                     MLKGIGDIINTSLVVIIACLIYMVLQNKLGSFELIILPVLVPIVSGGIGLITLPYIRK
                     ITQAIGNVIHSFTDLNPLLMSILISVAFSLLMVTPISLVAIATAISLNGLGSGAANLG
                     IVAACVTFLFGSLRVNSIGVNAVLLIGAAKMMIPVYLKNLIISIPLTINGIITGIIAY
                     VLQVKGTPLSAGFGYTGLVGPINAFNRMSGDPTMNIILLALGYFVIPFVSAFIVHEL"
BASE COUNT          441 a          212 c          142 g          347 t
ORIGIN      
        1 acaattcatg aacaataaat gccgatacaa atggtatgac aaaataacca agtgctaata
       61 aaataatatt cattgttgga tcacctgaca ttcggttaaa tgcatttatt gggccaacta
      121 atcctgtata tccaaatcca gcagacaatg gtgttccttt aacctgaaga acgtaagcaa
      181 tgattccagt aattattcca tttatagtca atggtatcga aataattaaa tttttcaagt
      241 aaactgggat catcattttt gcagctccta tgagtaaaac tgcatttaca ccaatagaat
      301 tgacacgcaa tgagccaaat aagaaagtaa cacaggcagc cactatacct aggtttgctg
      361 ctccacttcc tagtccgttt aaactaatcg cagttgcaat cgctactaac gatattggtg
      421 ttaccattaa taatgaaaat gccacactaa taagtataga cattaacaac ggatttaagt
      481 ctgtaaaaga atgaattaca tttccaattg cttgagtaat ttttcgaatg taaggtaatg
      541 tgattagacc gataccccca ctaacgatag gtactaaaac tggtaatata attaattcaa
      601 aagatccaag tttgttttgt aataccatat atataagaca tgcaataata acaaccaaac
      661 tcgtatttat aatgtcacct atacctttta acataaaact attattgcta tatacaacag
      721 cacctgaacc aatcatagct gatgtaccta ctatagcagc acctgcacca ttaaatttaa
      781 attgatgagc agctaaaacc ccaataataa atgccataaa tgattgaatt agtatcacta
      841 actgatacgt taattctaaa atttcattac cacttttaaa tatttttaat acttcaccta
      901 ataaagcatt cggaacaagt gcaataacaa caccagcacc aatagaattt aaaatcttac
      961 tgaaaaactg tttattatca gcattatttg cgttactcat aaacgacctc caatttgaac
     1021 attaaactca tcatattata gaaaattaac tttaacaatg attagttggc taatttaatc
     1081 taaatataac aatccacaaa ttgaataaag caaatttaaa caatctactc actaatcaat
     1141 gt
//
LOCUS       DAMZKC010000323         1131 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.328, whole genome shotgun sequence.
ACCESSION   DAMZKC010000323 DAMZKC010000000
VERSION     DAMZKC010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1131
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.328"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(<1..>1131)
                     /locus_tag="POB55_002770"
     CDS             complement(<1..>1131)
                     /locus_tag="POB55_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001624188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501667.1"
                     /translation="YASMFIYIVFIPLIPRTKLLINPMDKQLLYRSSLREVDIFNLIY
                     ASDVLKKFVGYFNLLIVGMAISLYHISPFIFNLKVFCALIYFSTISYVIQIIFINIKV
                     ISTKKLFSYHYCVFNFMMGSIVSVIGFVVSFLLVNLLIKPFLVFSTYVIKSKKTFEWY
                     SYFQDIKNEVIIINEKVMQLLDYISPHTIFMRLNFVNISFVIITIALFIALYYFNKIG
                     FWYRKEDIYLSSTNINFSLPISIKAITTIQLKHWISNKEEVNLHKPFFYISYSIWFFL
                     GCLIYFSQMAYNQLANAVIFILILNTITRDSFSAGTDFFTKSLRFDSDRKSIGLYRMS
                     NTDFKQIYDSKLSLIRFIGFKETILAIILLAIFLNQDIYLYI"
BASE COUNT          472 a          147 c          126 g          386 t
ORIGIN      
        1 ttatatataa atatatgtct tgatttaaaa atattgctaa taaaataata gcaagtattg
       61 tttctttaaa accaataaag cgaattagag ataatttaga atcgtaaatt tgtttaaaat
      121 cagtgtttga catcctatac aaaccgatac tttttctatc tgaatcaaaa cgtaatgatt
      181 tagtaaaaaa atccgtcccc gcagaaaaac tatcacgagt tatagtgttt aatatcaaaa
      241 taaaaataac tgcattagct aactgattat atgccatctg tgaaaaatag attaaacacc
      301 ctaaaaagaa ccatattgaa taactaatat agaagaaagg tttatgtaaa ttaacttctt
      361 ctttgttact tatccagtgt tttagctgta ttgttgttat agcttttatt gaaattggca
      421 acgaaaaatt aatatttgtt gaagatagat agatatcttc ttttctatac caaaacccta
      481 ttttattaaa ataatataac gctataaata aagctattgt aataatcaca aatgatatat
      541 tcacaaagtt taatctcata aaaatagtgt gaggtgatat ataatctaat aattgcataa
      601 ctttttcatt aataataatg acttcgttct ttatatcctg aaaataacta taccattcaa
      661 aagttttctt actttttatt acatatgtcg aaaaaactaa aaagggcttt attaataaat
      721 ttactaaaag aaatgagacg acaaatccaa taacagaaac aatgcttccc atcataaaat
      781 taaaaacaca atagtgataa gaaaataatt tttttgtgct tatgacttta atatttataa
      841 aaataatttg aattacataa gagattgttg aaaagtatat tagtgcacaa aaaaccttga
      901 ggttaaatat aaaaggacta atatgatata aactaatagc cataccaact atcaacaaat
      961 taaaatatcc aacaaacttt tttaaaacgt ctgaagcata tatcaagtta aaaatgtcaa
     1021 cttctctcaa actagatcta tataacagct gtttatccat tggattgatc aataatttag
     1081 ttctaggtat taatggtata aatacaatgt aaataaacat actcgcatag a
//
LOCUS       DAMZKC010000324         1116 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.329, whole genome shotgun sequence.
ACCESSION   DAMZKC010000324 DAMZKC010000000
VERSION     DAMZKC010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.329"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..465
                     /locus_tag="POB55_002771"
     CDS             <1..465
                     /locus_tag="POB55_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HDI6501668.1"
                     /translation="IDKENIIGAYCTIVFNDGRDNYIEVMTIEQIKQAWMQSSMIKDE
                     KALQNSKTHNNFKEEMAKKTVINRAAKRYINTSTDSNLFKYAQESEQRQRKEVLDAEV
                     EENANQEQLDFEQPVLEEAQYTELENDKPIDVSDFEEIKEPATEKESEEEPF"
     gene            546..>1116
                     /locus_tag="POB55_002772"
     CDS             546..>1116
                     /locus_tag="POB55_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDI6501669.1"
                     /translation="MKFERVQKHFKYKTRHIAGCLITHEHGDHAKYTKQFVDNGVISY
                     MTAGTQQAMNFESHRLCTIKAKQELRIGTWSILPFDIEHDANEPVAFLLQSTLGYKIL
                     YVTDTKYLKYKFNGITHMMLEVNYIYEQMQENIKNGSVHSALANRIMESHFSLEHAIG
                     MLKANDLTRLEEIHLIHLSSQNSNAKYIKR"
BASE COUNT          455 a          180 c          201 g          280 t
ORIGIN      
        1 atcgacaaag aaaacattat cggtgcgtac tgcacaatcg tatttaatga tggacgagat
       61 aactatattg aagtcatgac tattgaacaa attaaacaag catggatgca gtcatcaatg
      121 attaaagatg aaaaagcatt acaaaattct aaaacacata ataatttcaa agaagaaatg
      181 gctaaaaaaa cagttatcaa tagagctgct aaacgttata tcaacacatc aacagatagc
      241 aatcttttca aatacgcaca agaatccgaa caacgtcaac gcaaagaagt gttggacgca
      301 gaagttgaag aaaatgcaaa tcaagaacaa ttggactttg aacaaccagt tcttgaagaa
      361 gcacaataca cagaattaga aaatgataag cctattgatg tatctgactt tgaagaaata
      421 aaagaacctg caacagaaaa agaaagcgaa gaagagccat tttaattgaa acaatagcaa
      481 ctggttcaag tggtaactgc tacgtcttaa atgatggacg tactacgtta ctacttgagg
      541 caggtataaa atttgaacgt gttcaaaagc atttcaaata taaaacaaga catatagcag
      601 ggtgtcttat cacacacgaa catggtgatc atgcaaagta tacaaagcag tttgtcgaca
      661 atggtgtaat cagctatatg actgctggaa cacaacaagc tatgaatttt gaaagtcatc
      721 gcttatgcac gattaaggca aagcaagagc tgcgaatagg tacatggtca attctaccgt
      781 ttgacatcga acatgatgct aacgagcctg tggctttctt attacaaagc acactaggtt
      841 ataagatcct gtatgttact gatacgaagt atctgaaata caaatttaac ggcattacac
      901 acatgatgtt agaagttaat tatatctatg aacaaatgca agaaaacata aaaaacggca
      961 gtgtacacag cgcattagca aatagaatta tggagtctca ttttagctta gaacatgcta
     1021 taggaatgtt gaaagcaaat gatttaacta gactcgaaga aatacattta attcatttaa
     1081 gtagccaaaa ttcaaatgca aaatacatta aaagag
//
LOCUS       DAMZKC010000325         1115 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.330, whole genome shotgun sequence.
ACCESSION   DAMZKC010000325 DAMZKC010000000
VERSION     DAMZKC010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.330"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..355
                     /locus_tag="POB55_002773"
     CDS             <1..355
                     /locus_tag="POB55_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1672 family protein"
                     /protein_id="HDI6501670.1"
                     /translation="FYITYSSRSLKEYRKYYEPLIQKNDKEFKEGMERARKEVNYAAD
                     TDAVATLFSTKKNFTNDNTEDDVIELSDKLYNLKNKPDKSTITIQIGKPTINTKKAFY
                     DDNRPIEYGVHSKGE"
     gene            412..>1115
                     /locus_tag="POB55_002774"
     CDS             412..>1115
                     /locus_tag="POB55_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 family protein"
                     /protein_id="HDI6501671.1"
                     /translation="MFKRIKLILIATIILSGCSTTNNESNKETKSVPEEMEASKYVGQ
                     GFQPPAEKDAIEFAKKHKDKIAKRGEQFFMDNFGLKVKATNVVGSGDGVEVYVHCDDH
                     DIVFNASIPFDKSIIDSDSSLRSEDKGDDMSTLVGTVLSGFEYRAHKEELDNLTEVLK
                     EYKSKYKYTGYTENAIMKTQNSGFRNEYYYLTAIPYTLDEYKKYFQPLIKEDDKSFRD
                     GIRNSKKQLKDKSRPYV"
BASE COUNT          461 a          137 c          201 g          316 t
ORIGIN      
        1 tttttatatt acatactctt ctagaagttt aaaagaatat cgaaagtatt atgaaccttt
       61 gattcaaaaa aatgataagg aattcaaaga aggaatggaa cgagcaagaa aagaagtgaa
      121 ttatgccgct gatacagatg cagtagcgac actgtttagt acaaagaaaa attttactaa
      181 tgacaataca gaagatgatg taattgaact aagtgataaa ttatataatt taaaaaataa
      241 accagataaa tctacaatta caattcaaat aggtaagcct actattaata ctaaaaaagc
      301 cttttatgat gataatcgtc caatagaata tggggtgcac agtaaaggtg aataaaattt
      361 taaaaaattg cttttaatat tctaaacaaa cacataaatg ggagatgagc tatgtttaaa
      421 agaattaaac taatcttaat agcaacgata atactatcag gatgttcaac taccaataac
      481 gaatctaata aagaaacaaa atcagtacca gaagaaatgg aagcttcaaa atatgtaggt
      541 caaggcttcc aaccacctgc agaaaaagat gcgattgaat ttgcgaagaa gcataaagat
      601 aaaattgcta aacgtggcga acaatttttt atggataact tcggtctaaa agttaaagct
      661 acgaatgttg taggtagtgg cgacggcgta gaagtatacg tgcattgtga tgaccacgat
      721 atcgtattta atgcgagtat tccatttgac aaatcaatta ttgacagtga tagctcatta
      781 agaagtgagg ataaaggcga tgatatgagt actttagttg gtacagtgtt gagtggcttt
      841 gaatatcgag cacataagga agagttggat aatttaacag aagttttaaa agaatataaa
      901 agtaaatata aatatactgg ttatactgaa aatgcaatta tgaaaacgca gaatagtggt
      961 tttagaaatg agtattatta tttgactgca attccatata ctttagatga atataaaaag
     1021 tattttcaac ccttaatcaa agaggatgat aagtctttta gggacggtat cagaaactca
     1081 aaaaaacaac ttaaagataa atcacgtcca tatgt
//
LOCUS       DAMZKC010000326         1094 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.331, whole genome shotgun sequence.
ACCESSION   DAMZKC010000326 DAMZKC010000000
VERSION     DAMZKC010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.331"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..337
                     /locus_tag="POB55_002775"
     CDS             <1..337
                     /locus_tag="POB55_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6501672.1"
                     /translation="LLMVWLSYPLVTAIIGCLFVYIILLQMAQFYSQQAYGLWPQVWP
                     VPEEKVIKGYEQFLYRLMFVICTVFAVTFIIKHMTLFYVVLIFYIVGLLTIRSIIKKL
                     KYQETLLRD"
     gene            complement(461..964)
                     /gene="traP"
                     /locus_tag="POB55_002776"
     CDS             complement(461..964)
                     /gene="traP"
                     /locus_tag="POB55_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein TRAP"
                     /protein_id="HDI6501673.1"
                     /translation="MKKLYTSYGTYGFLHQIKINNPTHQLFQFSASDTSVIFEETDGE
                     TVLKSPTKYDVIKEIGEFSEHHFYCAIFIPSTEDHAYQLEKKLISVDDNFRNFGGFKS
                     YRLLRPAKGTTYKIYFGFADRHAYEDFKQSDAFNDHFSKDALSHYFGSSGQHSSYFER
                     YLYPIKE"
BASE COUNT          359 a          148 c          186 g          401 t
ORIGIN      
        1 tttattaatg gtttggttat catatccatt agtgacagca attattgggt gtttatttgt
       61 atatattata ttgttacaaa tggcacaatt ttattcacag caagcatatg gcttatggcc
      121 tcaagtgtgg cctgtacctg aagagaaggt aatcaaaggg tatgaacagt ttttatatag
      181 attaatgttt gttatttgta ctgtgtttgc agtgacattt attataaaac atatgacgtt
      241 attctatgtt gtactcattt tctatattgt aggtttatta acgattagaa gtattattaa
      301 aaagttgaaa tatcaggaaa cattattacg agactaaaaa acttccattg gcatgtatgt
      361 tgtaaaggtg catgtaatgt tgaacgccaa atgatacggc gttcagatta cattagcatc
      421 tatacgttaa cagcataacc aatggaagtt ttcttcgaat ctattctttt attgggtata
      481 gatatctttc aaaataactt gaatgttgtc cgcttgaacc aaagtaatga cttaatgcgt
      541 cttttgaaaa atggtcatta aaggcatcag attgcttaaa gtcttcgtat gcatgtcgat
      601 cagcaaatcc aaaataaatt ttgtatgttg tacctttagc aggtcttaac aaacgatagc
      661 ttttaaagcc accaaagttt ctgaaattat cgtctacact aatcagtttc ttttcaagtt
      721 gatatgcatg atcttctgtt gatggaatga agattgcaca atagaaatga tgttcactga
      781 attcaccaat ttctttgata acatcatatt ttgtaggtga ttttaaaaca gtctcaccat
      841 cagtttcttc aaaaataact gaagtatctg atgctgaaaa ttggaatagt tgatgggtcg
      901 ggttattgat ttttatctga tgtaaaaatc cataagtgcc ataagatgta tatagtttct
      961 tcatatgaaa ggtccccctt atgtttttat ttattatacg atgataaact agtcattacc
     1021 actattaata attgattaaa attactataa tgaatcttgc gagatattta gaagtaatga
     1081 tacaaaataa tgga
//
LOCUS       DAMZKC010000327         1088 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.332, whole genome shotgun sequence.
ACCESSION   DAMZKC010000327 DAMZKC010000000
VERSION     DAMZKC010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.332"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(178..266)
                     /locus_tag="POB55_002777"
     tRNA            complement(178..266)
                     /locus_tag="POB55_002777"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(229..231),aa:Ser,seq:gct)
     gene            complement(269..343)
                     /locus_tag="POB55_002778"
     tRNA            complement(269..343)
                     /locus_tag="POB55_002778"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(309..311),aa:Asn,seq:gtt)
     gene            complement(485..1054)
                     /locus_tag="POB55_002779"
     CDS             complement(485..1054)
                     /locus_tag="POB55_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComK"
                     /protein_id="HDI6501674.1"
                     /translation="MYSQNIYVIRKGDMVIRPAFDDDDQRNGSEIIRFDKTRIQNPFK
                     VQKIIERSCKFYGNTYLGKKAETNRITGISSKPPILLTPLFPTYFFPTHSDRQNENIW
                     LNMHYIESIKELKNRKCKVTFINNESIILHVSYHSLWHQYNNSIFYYYMVDKQSRMIS
                     KNPDQPIDYNKATLNVFEALTRYSLFEDK"
BASE COUNT          375 a          169 c          174 g          370 t
ORIGIN      
        1 attgctatgt aataaattaa ttataagttc aacaagtata aaaacctttc ttagagaaga
       61 ttatctagga aaggttttta gaattttaaa attgatataa aagaacagtg aaattctcat
      121 tcattatata taagtaaaca aaaaagctaa aatggtatat accattttag caagtaacgg
      181 aaacggaggg attcgaaccc tcgcgccgct ttcgcgacct acacccttag caggggcgcc
      241 tcttcagcca acttgagtac gtttccaatg gctccacagg taggactcga acctacgacc
      301 gatcggttaa cagccgatag ctctaccact gagctactgt ggattaattt gtatgtgttt
      361 gaatatcaag cacaaatatt attataacaa gattaagtta atttgcaata gataccttaa
      421 aaaaatatgc agaacattta cactatattt ataaaacatt ccgcatattt taaaaataaa
      481 caatttattt atcttcaaat aaagaatagc gtgtcaatgc ttcaaacaca ttcaatgtgg
      541 ctttattata atctattggt tggtcgggat tttttgatat catgcgagat tgtttatcta
      601 ccatgtaata gtaaaaaatg gaattgttat attgatgcca taaactgtgg tatgaaacat
      661 gaagaatgat tgattcatta ttaataaatg tcactttaca tttacgattt tttaattctt
      721 taatactttc gatataatgc atatttaacc aaatattttc attttgtctg tcagaatgtg
      781 ttgggaaaaa ataagttgga aataatggtg ttagtaaaat aggtggttta ctagaaatgc
      841 cagtaatgcg gtttgtctct gctttcttgc caagataagt attaccataa aatttgcaag
      901 agcgttcaat gattttctgg actttaaaag gattttgaat acgcgttttg tcaaaccgaa
      961 ttatttcact accgtttctt tgatcgtcat catcaaatgc tggtcgaata accatgtctc
     1021 ctttgcgtat cacataaata ttttgagaat acataagcaa aacctcgctt tattaagttt
     1081 taaaccat
//
LOCUS       DAMZKC010000328         1085 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.333, whole genome shotgun sequence.
ACCESSION   DAMZKC010000328 DAMZKC010000000
VERSION     DAMZKC010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.333"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            642..821
                     /locus_tag="POB55_002780"
     CDS             642..821
                     /locus_tag="POB55_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501675.1"
                     /translation="MKFKTTKECKSNNIFKRSQEINNRESEKGCLWGISMLILLFLLI
                     LFGITACSSSIHFIN"
BASE COUNT          408 a          131 c          147 g          399 t
ORIGIN      
        1 taattaattt aaatttagtt aatcaatttt gcatctattt tgttgtaagc tatataaaag
       61 gagtgataat gatggtgaaa aaaaacaaaa tccaattcac taaaaaaagt tgcaacactt
      121 gcattagcaa atttattatt agttggtgca cttactgaca atagtgccaa agccgaatct
      181 aagaaagatg atactgattt gaagttagtt agtcataacg tttatatgtt atcgaccgtt
      241 ttgtatccaa actggagact tttaacataa aattacttat cattcaaaaa gtaaaacagc
      301 ataatatcaa ggtttataac tttatcatta tcaataatac ctcatataaa ataaaatttt
      361 agggactttt tagggacttt aaatttaaaa ttacaagttt aatagaaaca tcaaaataat
      421 cacatgtttg tgtggaatgt acaccccaaa agctagactg aaaaatctat tttttgaggt
      481 gtatttttat aggtaaatat aataaattag agtagacaac tcagaattcc aattttataa
      541 taatattgct tgacatatca aactagatag tactattttg aatatattat tataatcaaa
      601 aattcattgt aaacttttag acaaaaggaa gtaataaaaa cgtgaaattt aaaacaacta
      661 aagaatgtaa aagtaataat atctttaaaa gaagtcaaga aattaataat agagaaagtg
      721 aaaagggttg tttatggggc attagcatgt tgattctact tttcttattg attctgtttg
      781 gaataactgc ttgttcatca agcattcact ttattaatta gattttttta cttggaggta
      841 tcatgtgaag aaccatacaa atataattaa tatcttatta gttatagtca actcattaac
      901 tcattttcta actctaaaca cctcattttt taataattca gcatcggatt tctgttttat
      961 cataggggct atatttttct taatcggaat ttttgttgca atatacggta tgaagcgagc
     1021 aacatattgg ttaaacttat tgattttatt taccaatatt ttttattttc tacacttctg
     1081 tgtgt
//
LOCUS       DAMZKC010000329         1083 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.334, whole genome shotgun sequence.
ACCESSION   DAMZKC010000329 DAMZKC010000000
VERSION     DAMZKC010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.334"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            64..681
                     /locus_tag="POB55_002781"
     CDS             64..681
                     /locus_tag="POB55_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HDI6501676.1"
                     /translation="MLCETEIINKNPKYRVIKYDDEYLMVDVISTWLVYFFPFINWFI
                     PKRCAKISREEYEKLNTVKPVKNKNFWPVVGGTILLGATSRKYIHLLNIQLEKRSVIF
                     ICFVVFLCILIFFVLLNRKLKLKVFDNKKEEQKIILVPTLKNAVLILYGYLLIGGMSI
                     LALSMLLTLENQNLITFIAWGMGLMLFFLMNITLIVNKTVKVIKR"
     gene            756..>1083
                     /locus_tag="POB55_002782"
     CDS             756..>1083
                     /locus_tag="POB55_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HDI6501677.1"
                     /translation="MLCESRVINQNPKYRIIKYNNEYFMVDLVSTWIAYFLPMINWFI
                     PKKYAKISREEFESLNIVKPAKNNTFWPVAGFAVLLTTLTRKYIYLLNIHLEKEIVIL
                     TCCMILL"
BASE COUNT          402 a          126 c          177 g          378 t
ORIGIN      
        1 ttttgagaaa cagaaacgat aatatagctg tgtgaatttg ttattaatag gaagtgataa
       61 ttattgcttt gtgaaaccga aattatcaat aaaaatccga aatacagagt tattaagtat
      121 gatgatgagt atttaatggt cgatgtaata agcacttggc ttgtatactt ttttccattt
      181 attaattggt tcattccaaa aaggtgcgcc aaaattagta gagaagagta tgaaaaactg
      241 aacactgtta agccagttaa aaataaaaat ttttggcctg ttgtaggagg aacaatccta
      301 ctaggtgcta cttccagaaa gtacatacac ttacttaata ttcaattaga aaaaagatcg
      361 gttatattta tatgcttcgt tgtatttcta tgtattttaa tcttttttgt gcttctgaat
      421 cgaaaattaa aattgaaagt tttcgataat aaaaaagaag aacaaaaaat aattttagta
      481 ccaacattaa aaaatgctgt tcttatatta tatggctatt tgttgattgg aggcatgtca
      541 atattagctt taagcatgtt gctgacatta gaaaaccaga atttaataac ttttatagct
      601 tggggtatgg ggttaatgtt gtttttcttg atgaatataa cactcattgt caataaaaca
      661 gtcaaagtta taaaaaggtg aataaataac taattatatt gtattggccg ttcttcaaat
      721 aaaaaataat ttgaataagg tggaagtgat aaacagtgct ttgcgaatct agagtcatta
      781 atcaaaaccc taaatataga attattaaat acaataatga atatttcatg gttgatttag
      841 taagtacttg gattgcttat tttttgccca tgattaattg gtttattccc aaaaagtacg
      901 cgaaaattag tagagaagaa tttgaaagtt taaatattgt caaacccgct aaaaataata
      961 ctttctggcc tgttgcagga tttgcagtgt tattaacaac cttaacaaga aaatatatct
     1021 atttgcttaa catccattta gaaaaagaaa tagttatatt aacatgctgt atgatacttc
     1081 tag
//
LOCUS       DAMZKC010000330         1069 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.335, whole genome shotgun sequence.
ACCESSION   DAMZKC010000330 DAMZKC010000000
VERSION     DAMZKC010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.335"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(1..479)
                     /locus_tag="POB55_002783"
                     /pseudo
     CDS             complement(1..479)
                     /locus_tag="POB55_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499902.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
     gene            complement(711..1069)
                     /locus_tag="POB55_002784"
                     /pseudo
     CDS             complement(711..1069)
                     /locus_tag="POB55_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001558444.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          393 a          151 c          157 g          368 t
ORIGIN      
        1 caatgttata ctcatttgaa tggtgaatca tttccaattc ttgatggtat ttatttagct
       61 cgtctaatat agttgtaatt tgtgccgggt ctatatttaa taaaataact ttgattggcg
      121 tgtcgatatt tgcaacatca atacaactgg ccagcttatg tggatctaaa cgctcaaaaa
      181 tcgcgttctc agcgtcaagt tgtgcagcat aattccaatc atcatcaata atataatcta
      241 actcatactt ttgaattatt tttaagatat ggtgatatgt atctgtatga attggtttga
      301 taacagaaat ctttgattgc tgtgaaatca tagcaccatt tgcgccaatt aatgtgtgct
      361 gatgcaaata taagttcatg accatcatgt tgtaattgta acaatgtatc aataatagtc
      421 tggtcaatta atcggccgtc aaaacaaagc gtaccatcaa tatcaaagac aaatctcatc
      481 aaatcactcc aaacaatata ataccatgat tatagcataa gtaagttatg tgacgatatt
      541 agataatgat tatgatgtag ggtacctttt gcgttacaca cacataactt cctataatat
      601 atattatgta aactagagtg tgagattcca taaggggact tatataactt cgagtttgta
      661 taagtcgttt tgattattag taaagtatgt ttgtagttac tattaacttt ttatttaaca
      721 catgctattt tatcaatacc tctattatta ggattagtac ataaataaac taaaattaac
      781 gcgacaaaat ttagaaaatc tataaaaata aataagattt gtgcagattg agtttgatat
      841 atgaccaaaa gtattgaaag taaaaaagta ggattaatat taattttaaa caagatataa
      901 ttaatggtaa acatctgaca actggttttc gttcttttaa taaagaacac gaaaagaata
      961 accacattat tatagaaaag ttacacacta gatatgtact aaaagaaata ccaccttctt
     1021 gtatgacata tctaaataag ttcacagcaa attcatgatt attatgaat
//
LOCUS       DAMZKC010000331         1056 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.336, whole genome shotgun sequence.
ACCESSION   DAMZKC010000331 DAMZKC010000000
VERSION     DAMZKC010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.336"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..>1056
                     /locus_tag="POB55_002785"
     rRNA            <1..>1056
                     /locus_tag="POB55_002785"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          295 a          207 c          313 g          241 t
ORIGIN      
        1 ttgtctctct tgagtggatc ctgagtacga cggagcacgt gaaattccgt cggaatctgg
       61 gaggaccatc tcctaaggct aaatactctc tagtgaccga tagtgaacca gtaccgtgag
      121 ggaaaggtga aaagcacccc ggaaggggag tgaaatagaa cctgaaaccg tgtgcttaca
      181 agtagtcaga gcccgttaat gggtgatggc gtgccttttg tagaatgaac cggcgagtta
      241 cgatttgatg caaggttaag cagtaaatgt ggagccgtag cgaaagcgag tctgaatagg
      301 gcgtttagta tttggtcgta gacccgaaac caggtgatct acccttggtc aggttgaagt
      361 tcaggtaaca ctgaatggag gaccgaaccg acttacgttg aaaagtgagc ggatgaactg
      421 agggtagcgg agaaattcca atcgaacctg gagatagctg gttctctccg aaatagcttt
      481 agggctagcc tcaagtgatg attattggag gtagagcact gtttggacga ggggcccctc
      541 tcgggttacc gaattcagac aaactccgaa tgccaattaa tttaacttgg gagtcagaac
      601 atgggtgata aggtccgtgt tcgaaaggga aacagcccag accaccagct aaggtcccaa
      661 aatatatgtt aagtggaaaa ggatgtggcg ttgcccagac aactaggatg ttggcttaga
      721 agcagccatc atttaaagag tgcgtaatag ctcactagtc gagtgacact gcgccgaaaa
      781 tgtaccgggg ctaaacatat taccgaagct gtggattgtc ctttggacaa tggtaggaga
      841 gcgttctaag ggcgttgaag catgatcgta aggacatgtg gagcgcttag aagtgagaat
      901 gccggtgtga gtagcgaaag acgggtgaga atcccgtcca ccgattgact aaggtttcca
      961 gaggaaggct cgtccgctct gggttagtcg ggtcctaagc tgaggccgac aggcgtaggc
     1021 gatggataac aggttgatat tcctgtacca cctata
//
LOCUS       DAMZKC010000332         1040 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.337, whole genome shotgun sequence.
ACCESSION   DAMZKC010000332 DAMZKC010000000
VERSION     DAMZKC010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.337"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            12..764
                     /gene="sarS"
                     /locus_tag="POB55_002786"
     CDS             12..764
                     /gene="sarS"
                     /locus_tag="POB55_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarS"
                     /protein_id="HDI6501678.1"
                     /translation="MKYNNHDKIRDFIIIEAYMFRFKKKVKPEVDMTIKEFILLTYLF
                     HQQENTLPFKKIVSDLCYKQSDLVQHIKVLVKHSYISKVRSKIDERNTYISISEEQRE
                     KIAERVTLFDQIIKQFNLADQSESQMIPKDSKEFLNLMMYTMYFKNIIKKHLTLSFVE
                     FTILAIITSQNKNIVLLKDLIETIHHKYPQTVRALNNLKKQGYLIKERSTEDERKILI
                     HMDDAQQDHAEQLLAQVNQLLADKDHLHLVFE"
BASE COUNT          405 a          159 c          127 g          349 t
ORIGIN      
        1 gagataaaac aatgaaatat aataaccatg acaaaattag agattttata atcattgaag
       61 catatatgtt tcgttttaag aaaaaagtca agcctgaagt cgatatgact ataaaagaat
      121 ttatattact gacttattta tttcatcagc aagaaaacac acttccattt aagaagattg
      181 tttcagattt atgttataaa caatcggatt tagtacagca tataaaagta cttgtgaaac
      241 attcatatat tagtaaagtt cgaagtaaaa ttgatgagcg taatacttac atttcaatat
      301 ctgaagaaca acgagagaaa attgcagaac gtgttacatt gtttgatcaa atcattaaac
      361 aatttaacct tgcagatcaa agtgaatcac agatgatacc aaaagatagt aaagaatttc
      421 taaacttgat gatgtataca atgtatttca agaatattat caaaaaacat ctaacattaa
      481 gttttgtaga attcacaatt ctagctatta tcacttctca aaataaaaac atcgttcttc
      541 ttaaagattt aattgaaaca atccaccata aataccctca aactgttaga gctctcaata
      601 atttaaaaaa gcaaggctat ctaataaaag aacgctcaac tgaagatgaa agaaaaattt
      661 taattcatat ggatgacgcg cagcaagacc atgctgaaca attattagct caagtgaatc
      721 aattattagc agataaagat catttacatc ttgtttttga ataatatctc tattacgcaa
      781 gtgtgctgta ttctaaagtg cacttgtgtt ttctattttt taataaaacc tcagcacata
      841 atgaacaact ttctattttc tatatcactt aaaaccattt ccgaaattaa acctcagcac
      901 attcaaagcc ccactttatt cttaaaaata ttttttaact catatgtatt aaaccgcttt
      961 cattataaaa aatatctcta tattttatct gtttttatta atcgaaatag cgtgattttg
     1021 cggttttaag ccttttactt
//
LOCUS       DAMZKC010000333         1038 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.338, whole genome shotgun sequence.
ACCESSION   DAMZKC010000333 DAMZKC010000000
VERSION     DAMZKC010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.338"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            134..>1038
                     /gene="bshC"
                     /locus_tag="POB55_002787"
     CDS             134..>1038
                     /gene="bshC"
                     /locus_tag="POB55_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HDI6501679.1"
                     /translation="MDCKVVSLNEKDQFIPKIKSSDPVITGLFQYDAAQQTSFEKRMS
                     KENNGREAALANVIREYMSDLKLSSEQELNIQHLANGSKVVIGGQQAGLFGGPLYTFH
                     KIFSIITLSKELTDTHKQQVVPVFWIAGEDHDFDEVNHTFVYNENHGSLHKVKYHTME
                     MPETTVSRYYPDKAELKQTLKTMFIHMKETVHTQGLLEICDRIIDQYDSWTDMFKALL
                     HETFKAYGVLFIDAQFEPLRKMEAPMFKKILKKHQLLDDAFRATQQRTQNQGLNAMIQ
                     TDTNVHLFLHDENMRQLVSYDGKHF"
BASE COUNT          388 a          142 c          195 g          313 t
ORIGIN      
        1 ttgatttgaa aatgtaaaac aatgaatatt ataaattttc catgattaaa ttgattttca
       61 agccgtaaaa ttgtcaatca ccggtataga ctttatacta tagtttgtaa tcgcgtactt
      121 aaggagaaaa aacatggact gtaaagtagt tagtttaaat gaaaaagatc agtttatacc
      181 aaaaataaag agcagtgacc ctgtaataac aggattattt caatatgatg cagctcaaca
      241 aactagtttt gaaaaaagga tgtctaaaga aaataatgga agagaagcgg cattagcgaa
      301 tgttattcgt gaatatatga gtgatttaaa gctttcaagt gaacaagaat taaacataca
      361 acatttagct aatggttcaa aagttgtgat tggtggacaa caagcagggc ttttcggggg
      421 accattgtat acattccata aaatattttc aatcattact ttatctaagg aattaacgga
      481 tacacataag caacaagtag taccagtttt ttggattgca ggagaagatc atgatttcga
      541 tgaagtgaat catacatttg tttataacga aaatcatggg tcgctgcata aggttaaata
      601 tcatacaatg gagatgccag agacgactgt ctctagatat tatcctgata aggctgagtt
      661 gaaacaaact ttaaaaacga tgttcattca tatgaaagaa actgttcata cacaaggtct
      721 actggagatt tgtgacagaa ttattgacca atatgactcg tggactgata tgtttaaagc
      781 actactgcat gaaacattta aagcatatgg cgttctattt atagatgcgc agtttgagcc
      841 gttaagaaaa atggaagcgc ctatgtttaa aaagattttg aaaaaacatc agttgcttga
      901 tgatgctttt agagcaacac aacaacgtac tcaaaatcaa ggcttgaatg cgatgataca
      961 aacagataca aatgttcatt tattcttaca tgatgaaaat atgcgtcaat tagtttcgta
     1021 tgatggtaag cattttaa
//
LOCUS       DAMZKC010000334          991 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.339, whole genome shotgun sequence.
ACCESSION   DAMZKC010000334 DAMZKC010000000
VERSION     DAMZKC010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..991
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.339"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            60..>991
                     /locus_tag="POB55_002788"
     CDS             60..>991
                     /locus_tag="POB55_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HDI6501680.1"
                     /translation="MGYRIETRTFEELEGKLKLPTFQRALVWSNNQKQEFFSTLKDGF
                     PFGSILLYEYENDNKYSLIDGLQRYSTMVDFMENPTNYIEFEDFTLKIAELYKGASES
                     TKAEVVNYAEDILLKIIEKYIDSTKENVKVNELAKKMVDKFPVLGETDIRDEITQIQS
                     DVITYFNNQLDISNVKIPCIFFEGEETELAEVFQRLNSGGKKLSKYQVFAAHWDRYEV
                     ELGDLKYSDEILKNVIERYERLNNDRGILIENFDPKEMETSRVINLSELCYGLGKVIS
                     DELQAFFKESTEDICNELGFQTMLIVFGIPTNKM"
BASE COUNT          386 a           98 c          193 g          314 t
ORIGIN      
        1 gcaattttta tagttttata gctatttaat tcgtatttga gataaaagga gaggtttgga
       61 tgggatatag aatagaaaca agaacgtttg aagagttaga aggaaaatta aagttaccaa
      121 cttttcaaag agcactagtt tggtctaata atcaaaagca agaatttttc tcaacgctga
      181 aagatggttt tccttttggt tctattctgt tatacgaata tgaaaatgat aataaataca
      241 gtcttattga tggcctgcaa aggtattcta caatggtaga tttcatggag aatccaacaa
      301 actatattga atttgaagat tttacattga aaattgctga attgtataaa ggggcatcag
      361 agagtacaaa agcagaagta gttaattatg ccgaagatat tctgctgaaa attattgaaa
      421 aatatattga ttctacaaaa gaaaatgtaa aagtaaatga actagctaaa aaaatggtgg
      481 ataaatttcc agtattaggt gaaacagata ttagagatga aatcactcaa atacagtcag
      541 atgttattac ttattttaat aatcaactag atattagtaa tgtcaagatt ccgtgtatct
      601 tctttgaagg agaagagact gaattggcag aagtttttca aaggttaaac agtggaggta
      661 aaaagttatc gaagtatcaa gtttttgctg cacattggga cagatatgaa gttgaattgg
      721 gagatttaaa gtatagtgat gagatactta aaaatgtaat agaaaggtac gaaagattaa
      781 ataatgatag aggtatctta attgaaaatt ttgaccctaa agaaatggag acgtcaagag
      841 taattaattt atctgaatta tgttatggac ttggtaaagt gatttctgat gaattacaag
      901 cattttttaa agagtcaaca gaagatatat gcaatgaatt aggatttcaa acaatgctaa
      961 ttgtatttgg cattcctaca aataagatga a
//
LOCUS       DAMZKC010000335          977 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.340, whole genome shotgun sequence.
ACCESSION   DAMZKC010000335 DAMZKC010000000
VERSION     DAMZKC010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..977
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.340"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            140..868
                     /locus_tag="POB55_002789"
     CDS             140..868
                     /locus_tag="POB55_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5067 domain-containing protein"
                     /protein_id="HDI6501681.1"
                     /translation="MKKVMGILLASTLILGACGHHQDSAKKESTSHKKKENDNEELNE
                     ELKEFKSKKNMDIKIKGDTIVSDKFEAKIKEPFIINEKDEKKKYIAFKMEITAKKDDK
                     DLNPSSISHDYINITQDDKNTVNKLRDGYLLSDKKYKDWTEHNQDQIKKGKTAQAMFI
                     YELRGDGNINLNVHKYSEDKTVDSKSFKFSKLKTEDFSHRAETREEVEKKEKEFEEEY
                     KKEQEREKEKEKQKDDDHSSLDEV"
BASE COUNT          452 a          110 c          148 g          267 t
ORIGIN      
        1 tacataggat cattattaca agaattattt aatatcatct tagaatctca caaaatatca
       61 actttgttta attactcaaa atgataagtc atttagagtt tttaaaataa atttttgcga
      121 aataaaggag acatgttaaa tgaaaaaagt aatggggata ttattagcaa gtacacttat
      181 cttaggtgct tgtggacatc atcaagatag tgcaaaaaaa gagagcacta gtcacaaaaa
      241 gaaagaaaat gacaatgaag aattaaatga agaacttaaa gaatttaaaa gcaaaaaaaa
      301 tatggatata aaaattaaag gcgatactat tgttagtgac aaatttgaag ctaaaataaa
      361 agaaccgttt atcatcaatg aaaaagatga gaaaaagaaa tatatcgctt ttaaaatgga
      421 aattactgct aaaaaagacg ataaagactt aaatccatct tctatttctc atgactatat
      481 taatatcact caagatgata aaaatacagt aaataaatta agagatggtt atcttttaag
      541 tgataaaaaa tataaagatt ggacagaaca taaccaagat caaattaaaa aaggcaaaac
      601 tgcacaagcc atgtttatct atgagttaag aggtgatgga aatattaatt taaatgtcca
      661 taaatactca gaagataaaa cagttgattc taaatcattc aaatttagta aacttaaaac
      721 cgaggatttt tctcatagag cggaaacaag agaagaagta gaaaagaaag aaaaagaatt
      781 tgaagaagag tacaaaaaag aacaagaacg agagaaagaa aaagaaaagc aaaaagatga
      841 cgaccacagt agtttagatg aagtataaat ttatgaatgc tcgcatacag cgttataatc
      901 tttgtaatta ttattgtcat tataaattag cttacctaca ctcataatga cacactaatt
      961 taattgttaa atacaac
//
LOCUS       DAMZKC010000336          961 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.341, whole genome shotgun sequence.
ACCESSION   DAMZKC010000336 DAMZKC010000000
VERSION     DAMZKC010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..961
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.341"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..465
                     /locus_tag="POB55_002790"
     CDS             <1..465
                     /locus_tag="POB55_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020807974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 family protein"
                     /protein_id="HDI6501682.1"
                     /translation="QKEKYDNLYKFFKENEKKYQYTGFTKEAINKTQNSGYENEYFYI
                     VANIPTLQEYRKYYEPLIKKNNLNFKKGMKQARKGVGYKAAIEVHTTLFSRSSNFSKD
                     KKLDDVIDLSESTKKLHLNFENTKIFLQLAKSTISTNRVNYSDNESIRIEVE"
     gene            523..>961
                     /locus_tag="POB55_002791"
     CDS             523..>961
                     /locus_tag="POB55_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 family protein"
                     /protein_id="HDI6501683.1"
                     /translation="MFKRTKLILIATLLLSGCSTTNNESNNETKSVPEEMEASKYVGQ
                     GFQPPAEKDAIEFAKKHRKEFEKVGEQFFKDNFGLKVKATNVVGKDDGIEVYVHCDDH
                     GIVFNASLPLYKDAIHQKGSMRSNDNGDDMSMMVGTVLSGFEYR"
BASE COUNT          396 a          125 c          178 g          262 t
ORIGIN      
        1 caaaaagaaa agtatgataa tttatataaa tttttcaaag aaaatgaaaa gaaatatcaa
       61 tatacaggat ttactaaaga ggcaattaac aaaacacaaa atagtggata tgaaaatgaa
      121 tatttttata tagttgctaa tataccgacg ctccaagaat ataggaaata ttacgaaccc
      181 ctaataaaga aaaataatct gaattttaaa aaaggtatga aacaagcaag gaaaggagta
      241 ggctataaag ctgcaataga agtacataca acattgtttt cgagaagtag taacttttca
      301 aaggacaaaa aattagatga tgttatagat ttgtctgaaa gtacgaaaaa gttacacctt
      361 aattttgaaa atacgaaaat atttttacaa ctagcaaaat ctactattag tactaatcga
      421 gttaattaca gtgataatga gtctataagg attgaggtcg aatgacttga aatcagctaa
      481 tttctctata ttctaaacaa acacataaat gggtgatgag ctatgtttaa aagaactaaa
      541 ctaatcttaa tagcaacatt actgctatca ggatgttcaa ctaccaataa cgaatccaac
      601 aatgaaacaa aatcagtacc agaagaaatg gaagcttcaa aatatgttgg acaaggcttc
      661 caaccacctg cagaaaaaga tgcgattgaa tttgcgaaga agcatcgtaa agaatttgaa
      721 aaagtaggtg aacaattctt taaagacaac tttggactga aggtaaaggc taccaatgtt
      781 gtaggtaaag atgatggtat agaagtttat gtgcattgcg atgatcatgg cattgtattt
      841 aatgcaagtt tgcccttgta caaagatgcc atccatcaaa aaggatcaat gcgtagtaat
      901 gacaacggtg atgatatgag tatgatggtg ggtacagtgc tgagtggctt tgaatatcga
      961 g
//
LOCUS       DAMZKC010000337          953 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.342, whole genome shotgun sequence.
ACCESSION   DAMZKC010000337 DAMZKC010000000
VERSION     DAMZKC010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..953
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.342"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..379
                     /locus_tag="POB55_002792"
     CDS             <1..379
                     /locus_tag="POB55_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDI6501684.1"
                     /translation="LTVNENLDIGLAYKKISKKEKEQLKIRYIEQFGLSNSLKRKVHT
                     LSGGEQQRVALIRMMLKDPIVMLADEPTGALDPKTGQMIIQSLFDLVDENKVLILATH
                     DMAIANQCDEIIDLEQYRKVASM"
     gene            complement(467..757)
                     /locus_tag="POB55_002793"
     CDS             complement(467..757)
                     /locus_tag="POB55_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501685.1"
                     /translation="MFYACHFKMKIGGVILTRTYNIIGILSCLISFIIMALPMIWYTA
                     SALWFFPGAIMILLLSLVIVFCYIKTKNQLHLLLIVLNIIILLFFSLPLLLS"
BASE COUNT          391 a          117 c          155 g          290 t
ORIGIN      
        1 tttgacagta aatgaaaatt tagatattgg attagcatat aaaaaaataa gtaagaaaga
       61 aaaagaacaa ttaaagatac gttatataga acagtttggt ctgtcaaaca gtttaaaaag
      121 aaaagttcat acgctaagtg gaggtgaaca acaacgtgtc gctttaatta gaatgatgtt
      181 aaaagatccg attgttatgt tagctgatga accaacgggt gcgttagatc ctaaaacagg
      241 acagatgatt attcaatcat tatttgattt ggtcgatgaa aataaagtgc tgattttagc
      301 aacacatgat atggctattg caaatcaatg tgacgaaata atagatttag aacagtatag
      361 aaaagtagca tctatgtgat atccaaaaga taaaattaaa agccaatcaa tgggaaatgt
      421 tgacttcaac actccgtttg attggctttt ataatgtgtt actaagttat gaaagtaaca
      481 acggcaaact gaaaaaaagc aatataataa tatttaatac gattagtaat aaatgtaatt
      541 gatttttcgt tttaatatag caaaaaacaa tgactaaaga taacaacaat atcatgattg
      601 cacctgggaa gaaccataat gctgaagctg tgtaccaaat cattggtaac gccataataa
      661 taaaagatat aagacaagaa aggataccaa taatattata agttctagtc aaaattacac
      721 ctccaatttt cattttaaaa tgacatgcgt aaaacaatca ttttaaatac atcatacaat
      781 aaaattaaat tgtgcggtag aagaatttga aattaaattg aattggctta tctttcgtaa
      841 acataattat gacgtaacaa tccttataag tggaacgagt gtagttatac catctgtata
      901 gagagtcgta tcaatcataa aatttaatct atgttgttat tgattttcat act
//
LOCUS       DAMZKC010000338          947 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.343, whole genome shotgun sequence.
ACCESSION   DAMZKC010000338 DAMZKC010000000
VERSION     DAMZKC010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..947
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.343"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            130..273
                     /locus_tag="POB55_002794"
     CDS             130..273
                     /locus_tag="POB55_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gallidermin/nisin family lantibiotic"
                     /protein_id="HDI6501686.1"
                     /translation="MEKVLDLDVQVKANNNSNDSAGDERITSHSLCTPGCAKTGSFNS
                     FCC"
     gene            338..>947
                     /locus_tag="POB55_002795"
     CDS             338..>947
                     /locus_tag="POB55_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic dehydratase"
                     /protein_id="HDI6501687.1"
                     /translation="MNTIYEPSSICMIRTPLLSVEFFNLFLNTEQIKYSDLQLNAQMK
                     ESILTTTFNLYCTLQEINFDGDNKKVRDAKESLLKYLIRMSTRPTPFGLLSGINLGHF
                     VNEPTRLKVGNSIQKYVKVDGEWLYKLVSYIESIDEYYQNLKVIWNSKAHIINDRIYL
                     NEQSAIYLNNNKDTSFSIKNSELLVFIKTTVTNNNITFSNLAE"
BASE COUNT          363 a          114 c          150 g          320 t
ORIGIN      
        1 taaattaata agtgtttaca aaaaattcac aaaatataat tatataattt attgatttgt
       61 tgtgtgaaaa gggataatat gacaattgaa gtaggcctta acttcaacta ataataggag
      121 gttttatcta tggaaaaagt tcttgattta gacgtgcaag ttaaagcaaa caataactca
      181 aatgattcag caggtgacga acgtattaca agtcatagtt tatgtactcc tggttgtgct
      241 aagactggta gttttaatag cttctgctgt taagattaca gcaaatagag cctgaaaggg
      301 ctctatttgt ctattcaaat atgtgaggtg cttacaaatg aacactattt acgaaccgtc
      361 aagtatttgt atgattagaa cacctttatt atcagttgaa tttttcaatt tatttttaaa
      421 tactgaacaa attaaatata gcgacttaca attaaatgct cagatgaaag aatcaatcct
      481 aacaacaaca tttaacttat attgtacttt acaagaaata aattttgatg gcgataacaa
      541 gaaggttaga gatgctaaag aaagtttatt aaaatattta attagaatgt caacacgtcc
      601 aactccattt ggattattaa gtggtattaa tttggggcat tttgtaaatg aaccaacacg
      661 tcttaaagtt ggaaatagta ttcaaaaata tgtgaaagtc gatggcgaat ggttgtataa
      721 actggttagt tatattgaga gtattgatga atattatcaa aatttgaaag ttatttggaa
      781 tagtaaagca catattatca atgatcgaat atatttaaat gaacaaagtg ctatttattt
      841 aaacaacaat aaagatacat ctttttccat taaaaatagc gaattactcg tattcatcaa
      901 aaccactgtg acgaataata atattacttt ttcaaattta gctgaaa
//
LOCUS       DAMZKC010000339          945 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.344, whole genome shotgun sequence.
ACCESSION   DAMZKC010000339 DAMZKC010000000
VERSION     DAMZKC010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..945
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.344"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..>945
                     /locus_tag="POB55_002796"
     CDS             <1..>945
                     /locus_tag="POB55_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDI6501688.1"
                     /translation="YASVLGATLRIDICEIHIEIANYIYEEIFDKEMVYPHILMRDYA
                     RQTIEYISLSKDISNINLEKIRPPYKSNWYKKEYSNLNIDDYIKSLKNKLDSHLHFSI
                     DKIKNSMTTEYGRGTGAYGDFGRYVFGYAVRNWVKGFKSDQDLSNIALMRIFEMGYDA
                     KLHGEFDMWVNRYDNFNNSIERISKKYQWIAYYEILAKLVDKFPDVQYSGLWDDYIRD
                     IDPTLLLLEIDKESKILVPSPLPSHQSNEWVKNTKVFDETKLFLEIDIDNHRYICLSS
                     KFNFEKREKEIPFEDRDSCYFLAMGYFYNKEDSNEIIKG"
BASE COUNT          376 a           96 c          159 g          314 t
ORIGIN      
        1 atatgcttct gttttagggg cgacacttcg aatcgacatt tgtgaaatac atatagagat
       61 tgctaattat atttatgaag aaatatttga taaagaaatg gtatatcctc atattttaat
      121 gagagattat gctaggcaaa caattgaata tattagttta tcaaaagata tatctaatat
      181 aaatttagaa aaaattaggc caccttataa aagtaattgg tataaaaaag aatattcgaa
      241 tttaaatata gacgattaca taaaatcttt aaaaaacaaa ttagatagcc atttacattt
      301 ttctatcgat aaaattaaaa actcaatgac aacggaatat ggaagaggaa ctggggcata
      361 tggcgatttt ggcagatatg tatttggtta tgctgtacga aactgggtaa agggttttaa
      421 aagtgatcaa gacttaagta atatagcatt aatgaggatt tttgaaatgg gttatgatgc
      481 aaaattacat ggtgaatttg atatgtgggt taatcgttat gataacttta ataattcaat
      541 tgaaagaatt agcaaaaaat atcaatggat tgcttactat gaaattttag ctaaacttgt
      601 agataaattt cctgatgttc aatattctgg actatgggat gattatatta gagatattga
      661 tcctacttta ctattacttg aaattgataa agaatcaaaa atactagtac cctcaccact
      721 tccttcgcat caatcgaatg aatgggtaaa aaataccaaa gtttttgatg aaacaaaatt
      781 atttttagaa atagatatag acaatcatag atatatatgt ttaagctcaa agtttaattt
      841 tgaaaagaga gagaaagaaa taccatttga agatagagac tcatgttatt tcttagcgat
      901 gggatatttt tataataaag aagatagtaa tgaaattatc aaagg
//
LOCUS       DAMZKC010000340          887 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.345, whole genome shotgun sequence.
ACCESSION   DAMZKC010000340 DAMZKC010000000
VERSION     DAMZKC010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..887
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.345"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(25..>887)
                     /locus_tag="POB55_002797"
     CDS             complement(25..>887)
                     /locus_tag="POB55_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HDI6501689.1"
                     /translation="PTFLFISGYFAKNIDKPYYLEKISKRLIVPYMIFFAFFSIYYFL
                     TGKSDELQLDPFNPVFALWFLITLFFFHVILVIVRRFNPYKVLSVSIIISIGAGFSDN
                     IDSYLSISRTIVFFPIFYLGYIFTKKHTAIFKNKKLIPVSIITFILFFIVYVIHPINA
                     DWLLGSSPYTSLENEGQSIFSPFKRLILYGIILIAMTAFLNLMSTKKKLYTYIGSRTL
                     YVYLLHGLIIGIVRGFEWYPFDNPISLMTYLYLISISVIIVYVLSTNFVCKWTNPIIN
                     LQRPSQFKDS"
BASE COUNT          353 a          111 c          127 g          296 t
ORIGIN      
        1 ttaatgtact tagacagtat aaaattatga atctttaaac tgtgatggtc tttgtaaatt
       61 aattattgga tttgtccatt tacaaacaaa gtttgtagat aatacatata cgatgattac
      121 agatatactt attaaataaa gataagtcat taatgatata ggattatcga atgggtacca
      181 ttcaaatcct ctaacaatgc caataattaa accatgtaat aaatatacgt atagcgtacg
      241 actaccaata taagtatata attttttctt tgttgacatt aaatttagaa acgcagtcat
      301 tgcgattaat ataattccat ataatataag tcgtttaaaa ggactgaata tactctgtcc
      361 ttcattttca agtgaagtat atggtgaact tcccaataac caatctgcat tgataggatg
      421 aatcacgtaa acgataaaaa acaaaataaa ggtaatgata gatactggta ttagtttttt
      481 atttttaaaa atagccgtat gttttttggt gaaaatgtaa cctagataaa atattgggaa
      541 aaatacgatt gtccttgaaa tgcttaagta gctatcgatg ttatctgaaa aacctgctcc
      601 aatagatata ataattgaaa ctgatagcac tttatatgga ttaaatcttc taactattac
      661 taaaatgaca tgaaagaaaa atagcgtgat caaaaaccat aacgcaaata ctgggttaaa
      721 aggatcaagt tgtaattcgt cacttttacc tgttaagaaa taataaattg aaaagaatgc
      781 aaaaaatatc atataaggta ctatcaaacg ttttgaaatt ttttctaaat agtatggttt
      841 atcaatattt ttcgcgaaat aaccagatat aaacaaaaat gttggca
//
LOCUS       DAMZKC010000341          880 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.346, whole genome shotgun sequence.
ACCESSION   DAMZKC010000341 DAMZKC010000000
VERSION     DAMZKC010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..880
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.346"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            229..330
                     /locus_tag="POB55_002798"
     CDS             229..330
                     /locus_tag="POB55_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501690.1"
                     /translation="MKFVANKLTLNIFRKFCINNEKEIWIKYEEVFL"
     gene            340..612
                     /locus_tag="POB55_002799"
     CDS             340..612
                     /locus_tag="POB55_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001790097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HDI6501691.1"
                     /translation="MFGTILYLTLALGLSTAAYASTEYAEGGTWSHGVGSKYVWSYYY
                     HGHKGHGATAIGKYRSFSGYTRAGVKAKASATKHNCWVNRAYYNIY"
     gene            656..>880
                     /locus_tag="POB55_002800"
     CDS             656..>880
                     /locus_tag="POB55_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HDI6501692.1"
                     /translation="MKWFKLILDVTTFILIAILLFVYTYKENEEILPDTKYPIAVTDW
                     NKKYSKNEIYKRINQFAKNENVAIYKSTSNY"
BASE COUNT          345 a          119 c          141 g          275 t
ORIGIN      
        1 ttaatataac tttcattgat atagaagtcg caatgattaa tttaaacaat aaaaaacaac
       61 catcatttca aaactttgac aacgaatgat ggttaaagaa aatctcattt agtcaccgtc
      121 tttttaacgg gttcaatagg ggacatgttc ctgcatgtcg tctatttatg cactgcgcaa
      181 tttaacaata atacaattat actttgatgt ccatttaaca taaagtggtt gaaatttgta
      241 gcaaataaat taaccttgaa tattttcaga aaattctgta taaataacga aaaagagata
      301 tggattaaat atgaagaggt gtttttatag aagaataaaa tttttggcac tatattatat
      361 ttaactttag cacttggatt atcaacagca gcttatgcat ctacagaata cgcagaagga
      421 ggcacttgga gtcacggtgt cggcagtaag tatgtttggt cttattatta tcatggtcat
      481 aaaggacatg gtgcaacagc tattggaaaa tatagatcat ttagtggtta tacaagagct
      541 ggtgtaaaag caaaagcatc agctactaaa cataattgct gggtcaatag agcgtattat
      601 aacatttatt aaatactaaa aaatcataga aaataaacaa cgcaagaggt acaacatgaa
      661 atggttcaaa ttaatacttg atgttacaac cttcattctg attgctatat tgttatttgt
      721 ttatacatat aaagaaaacg aagaaatatt gcctgatact aaatacccta tagcggtaac
      781 tgactggaat aaaaaatata gtaaaaatga gatttataaa cgtataaatc aattcgctaa
      841 aaatgagaac gtagcaatct ataaatcaac ttcaaattat
//
LOCUS       DAMZKC010000342          879 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.347, whole genome shotgun sequence.
ACCESSION   DAMZKC010000342 DAMZKC010000000
VERSION     DAMZKC010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..879
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.347"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..703
                     /locus_tag="POB55_002801"
     CDS             <1..703
                     /locus_tag="POB55_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HDI6501693.1"
                     /translation="YMEIISKYQVRTVTFADTVGCSTPLEYGDIFNYFVKKYSNIIFS
                     AHCHNDLGLATANTLAAILNGAKQIETTFLGIGERAGNAPIEEIITILTKKQIESTEF
                     TLPDVYKTSINISKILDFQISENKPIIGENIFKHESGIHQDGTKKNINMYQYLVPSDL
                     GLKNSQVVQFPISNISSKKILHNKFKSIVNTEEIDENISFYKLVNKFSPEVAPEDTVD
                     LLQIIKRRSKDGNFK"
     gene            687..>879
                     /locus_tag="POB55_002802"
     CDS             687..>879
                     /locus_tag="POB55_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HDI6501694.1"
                     /translation="MEILNDSVKSFEKLYLQLQDGFPVIVPTDTNYNLCSLPNNDLCI
                     DKIFEYKKRSKDKPLSLFID"
BASE COUNT          346 a          109 c          131 g          293 t
ORIGIN      
        1 atacatggaa attatttcaa aatatcaagt tagaacagtt acatttgcgg acactgtagg
       61 atgttcgaca ccattagaat acggagatat ttttaattac tttgtaaaaa aatattctaa
      121 cataattttt tctgctcatt gtcataacga tctagggttg gctactgcaa atacattagc
      181 tgcaatttta aatggtgcaa agcaaataga aactacattt ttgggaattg gtgagagagc
      241 gggtaatgct cctattgagg aaataattac tattttgaca aaaaaacaaa tagaaagtac
      301 ggaattcact ttacccgacg tatataaaac tagtattaat atttctaaaa ttctcgattt
      361 tcaaatatca gaaaacaaac ctataattgg tgaaaatata tttaaacatg aatcaggaat
      421 tcatcaagat ggtactaaaa aaaatataaa tatgtatcaa tatttagttc ctagtgattt
      481 aggattgaaa aattcacaag ttgttcagtt tccaataagt aatatttcta gtaagaaaat
      541 cttgcacaat aaatttaaat caatagttaa cactgaagaa attgatgaaa atatttcttt
      601 ctataaactt gtgaacaagt tttcacctga agtagcacct gaagatacag tggatttact
      661 tcagataata aaaaggagga gtaaagatgg aaattttaaa tgattctgta aaaagttttg
      721 aaaaacttta tttacaatta caagatggtt ttccagtgat tgttcctact gatactaatt
      781 ataatttatg tagtttacct aacaacgatc tttgtatcga taaaatattt gaatataaaa
      841 agcgatcgaa agataagcca ttatcattat ttattgata
//
LOCUS       DAMZKC010000343          869 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.348, whole genome shotgun sequence.
ACCESSION   DAMZKC010000343 DAMZKC010000000
VERSION     DAMZKC010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..869
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.348"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            104..841
                     /gene="sdpB"
                     /locus_tag="POB55_002803"
     CDS             104..841
                     /gene="sdpB"
                     /locus_tag="POB55_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease SdpB"
                     /protein_id="HDI6501695.1"
                     /translation="MENEKKKYTFKDIAWRDLLIIPIIGVIFYALTIAAYIGPILFGY
                     YEIDSNLIFVATITQALAYTIGIGAFYLFHLKVMPSRLRAGFAYIKKYWLRLSITYII
                     ALILIYTYEYMTQFLPKHLQYSETANELELNKMFEVPAFLPVAFLLIVIVGPIVEEIV
                     FRHILIGELGKKFNFIAMSIVSVFLFAFIHVTDAKSPFEFGPYLILSIILVFTYLKSG
                     RNLGSTIALHIANNFVSFVISVISIYA"
BASE COUNT          286 a          114 c          146 g          323 t
ORIGIN      
        1 tgatttgaat caaattagta atttgttgtc tcatatactt agtttcgttt atcatgtaga
       61 agtacctcca aaaatcaatt attaaacagg aaaggatata acagtggaaa atgaaaaaaa
      121 gaaatacacg tttaaagata tagcatggag agatttgctt ataattccaa ttataggtgt
      181 tatattttat gcactcacaa ttgcagctta cataggacca attttgtttg gttattatga
      241 aatcgatagt aatctgatat ttgtagcgac tatcacacaa gctttagcat atacgattgg
      301 gattggcgca ttttatttat ttcatttaaa agtgatgcca tcaaggcttc gagctggttt
      361 tgcgtatatc aaaaaatatt ggcttagatt gtcaataacg tatattattg ctctcatctt
      421 aatttataca tatgaatata tgacacagtt tctacctaaa catttgcaat atagtgaaac
      481 agccaatgaa cttgagctaa ataaaatgtt tgaagtgcct gcatttttac ctgtagcatt
      541 cttattaatt gtgattgtag gtccaatagt tgaagaaatc gtatttagac atattttaat
      601 tggagaatta ggtaagaagt ttaattttat agctatgagt attgtttcgg tgttcttatt
      661 tgcatttatt catgtgactg atgcaaagtc gccgtttgag tttggtccat atctcatact
      721 atccatcata ttagttttca cttatttaaa atctggaaga aacctaggtt caacaattgc
      781 attacatata gcaaataatt ttgtttcttt cgttatatca gtaataagta tttatgcgta
      841 gataaagagg tcgggacatt aagttttta
//
LOCUS       DAMZKC010000344          863 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.349, whole genome shotgun sequence.
ACCESSION   DAMZKC010000344 DAMZKC010000000
VERSION     DAMZKC010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.349"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
BASE COUNT          339 a          134 c          105 g          285 t
ORIGIN      
        1 actttatgaa aatggtataa caagaatcga aattatacgc gaatacgatt taacactctc
       61 aatcttctcg aactgaataa atactgggtc attcaatcat caagacaact tattaagtga
      121 tgataaatgt tcataccatt ttgcaaatat aaccagatac tctcaacaaa atgtagagtt
      181 caaaattaaa aatgtcccta attggcacat tgctgtatga tattaaattc aacttagatt
      241 tcaacaatat aactaatgaa gcttatattt accgcgatat caaaccaaac agcctatcca
      301 caaacaaatt agaatatacc atgttagtaa tagtaaatga tatatatctt ccaaagaccg
      361 caaactgtag cagaaaatga gaaccttgcg tttttgaaat ggtctaataa atcaaatatc
      421 attgaaactt taatcacata aattaacagt agtcccctgt tcgcagttct ataaattaca
      481 aatgcaataa aggctaaagt aaactaatta ctttagcctt tattaataat ttagtcgaat
      541 ttattaaaaa tcgactaact cctggttatg ctacgctttt ctttaaaatc cgcacgtaga
      601 tgccgattga ttatattata atattattat tttatttttt caagcgtata tataatgttt
      661 gattttcttt aagtaacagc taaacattac atttcaaaac gattaggtca tgcagatgta
      721 catacaacgc ttaaaacata ttcgcatttg attaaagaat ttgaagataa tgaaactaaa
      781 tttatagaaa aaaatctgaa tgatttgttt tgtgactaaa cttcaaaatc ttgtgactaa
      841 agttaagatt aatcatcaaa aat
//
LOCUS       DAMZKC010000345          848 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.350, whole genome shotgun sequence.
ACCESSION   DAMZKC010000345 DAMZKC010000000
VERSION     DAMZKC010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..848
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.350"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            120..575
                     /locus_tag="POB55_002804"
     CDS             120..575
                     /locus_tag="POB55_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001086052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501696.1"
                     /translation="MAKLDLNSLNDEHVKLLINELKYPETHIDVNELKTIVASRINER
                     QEIISFKLGIKYLLTIKRGNIEKDRFSISIIFKDTYHTLVRIDINGGTHDNPDGTIAP
                     KSHIHIYNDKYDKKDRFAYEINLKDFPDIYNLYNVYMSFLEYNNIKDLE"
     gene            588..>848
                     /locus_tag="POB55_002805"
     CDS             588..>848
                     /locus_tag="POB55_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1828 domain-containing protein"
                     /protein_id="HDI6501697.1"
                     /translation="MTTFDAKKLKKEYLDWYNQTLEFSNLSNNVVRIDTPFKDNSLDN
                     LIIYALYDQSRDMITLTDDGYTIFDLENNGISLNKSKKRKKIF"
BASE COUNT          347 a          102 c          125 g          274 t
ORIGIN      
        1 tgctaaattg ataatataca cttttggttg tgtatacatc tcacctatct ttttttgctt
       61 ttttcaaata agaaagtatt aaaaattaat gcctatacaa tgttaaagga ggaacagaca
      121 tggctaaact tgatttaaat agtcttaacg acgagcacgt aaaattatta ataaatgaat
      181 taaaatatcc agaaactcat atcgatgtaa atgaattaaa aacaatagtt gctagtcgaa
      241 taaatgaaag gcaagaaata ataagtttta agttaggaat aaagtactta ttaacgataa
      301 aaagagggaa catagaaaaa gataggtttt caatttcaat cattttcaaa gatacctatc
      361 acaccctagt tagaatagat attaacggtg gtactcacga taatccagat ggaacaatcg
      421 ctccgaaaag tcatattcac atatataatg ataagtatga taaaaaggat aggtttgctt
      481 atgaaattaa cttgaaagat ttccccgaca tctataactt gtataatgtg tatatgtcgt
      541 ttttagagta taataatata aaagaccttg aataaaggag ggataatatg actacatttg
      601 atgctaaaaa attaaaaaaa gaatatcttg attggtataa tcagacctta gagttttcta
      661 atttatcaaa caatgtagta agaatagata ctccttttaa agataattct ttagataatt
      721 taataattta cgctttatac gatcagtcca gagacatgat tacactgaca gatgacggct
      781 atactatatt tgatttagaa aataatggta tttctttaaa taaatcaaaa aaacgtaaaa
      841 agattttt
//
LOCUS       DAMZKC010000346          840 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.351, whole genome shotgun sequence.
ACCESSION   DAMZKC010000346 DAMZKC010000000
VERSION     DAMZKC010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..840
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.351"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(<1..>840)
                     /locus_tag="POB55_002806"
     CDS             complement(<1..>840)
                     /locus_tag="POB55_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDI6501698.1"
                     /translation="LNQHVCIIRLKKEYYYNFFGQYLLSRKGKRKIFLAQSGGSREGL
                     NFKEIANLKIFTPTIFEEQQKIGKFFSKLDRQIELEEQKLELLQQQKKGYMQKIFTQE
                     LRFKDENGEEYPEWENKFIKDIFIFENNRRKPITSSLREKGLYPYYGATGIIDYVKDY
                     LFNNEERLLIGEDGAKWGQFETSSFIANGQYWVNNHAHVVKSNDHNLFFMNYYLNFKE
                     LRAFVTGNAPAKLTHANLCNINLKIPCLTEQDKVSALLKSIDNKMNNQMNRIELLKER
                     KKEL"
BASE COUNT          241 a          142 c          106 g          351 t
ORIGIN      
        1 tagttctttt ttacgttctt ttaataactc aattctattc atttgattat tcattttatt
       61 gtctatagat tttaacaatg cacttacttt atcttgttca gtgagacaag gtattttaag
      121 atttatattg cataagttcg catgagttaa tttagctggt gcattacctg tgacaaatgc
      181 tcgtagttct ttaaaattta aataataatt cataaaaaac aaattatgat cattactttt
      241 aactacatgc gcatgattat ttacccagta ttgcccatta gcaataaagc tactcgtctc
      301 aaactgcccc cattttgcac catcttctcc tattagtaat cgttcttcat tattgaataa
      361 ataatctttt acgtaatcaa taattccagt tgcaccatag taagggtata accccttttc
      421 tcttaatgaa gaagtaattg gttttcttct attattttca aagataaaga tgtcttttat
      481 gaatttgttt tcccactctg gatattcttc accattctca tctttgaatc gcaattcttg
      541 tgtgaagatt ttctgcatat agcctttttt ctgttgttga agcaattcaa gtttttgttc
      601 ttctaattca atttgtcggt cgagtttgct gaagaacttg cctatttttt gctgttcttc
      661 aaatatagtt ggggtgaaga tttttaaatt agcaatttct ttgaagttta gaccttctcg
      721 actacctcca ctttgtgcaa ggaaaatttt ccttttacct tttcttgata atagatactg
      781 tccaaaaaaa ttataataat actctttttt caatctaata atacatacat gttgatttaa
//
LOCUS       DAMZKC010000347          840 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.352, whole genome shotgun sequence.
ACCESSION   DAMZKC010000347 DAMZKC010000000
VERSION     DAMZKC010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..840
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.352"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            95..>840
                     /locus_tag="POB55_002807"
     CDS             95..>840
                     /locus_tag="POB55_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001623710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAP domain-containing protein"
                     /protein_id="HDI6501699.1"
                     /translation="MKFKSLITTTLALGVIASTGANFNTNEASAAAKPLDKSSSTLHH
                     GHSNIQIPYTITVNGTSQNILSSLTFNKNQNISYKDIENKVKSVLYFNRGISDIDLRL
                     SKQAEYTVHFKNGTKRVIDLKSGIYTADLINTSDIKAISVNVDTKKQPKDKAKANVQV
                     PYTITVNGTSQNILSNLTFNKNQNISYKDLEGKVKSVLESNRGITDVDLRLSKQAKYT
                     VNFKNGTKKVIDLKSGIYTANLINSSDIKS"
BASE COUNT          358 a          116 c          125 g          241 t
ORIGIN      
        1 tttattatta atattcagtt aattcaaaaa atagagaaag tctggctata ataaagttgc
       61 aatcacgaat tacataaaaa aggagagata atttatgaaa tttaagtcat tgattacaac
      121 aacattagca ttaggcgtta tagcatcaac aggagcaaac tttaatacta acgaagcatc
      181 tgccgcagct aagccattag ataaatcatc aagtacatta caccatggac attctaacat
      241 ccagattcca tatacaatta ctgtgaacgg cacaagccaa aacattttat caagcttaac
      301 atttaataag aatcaaaata ttagttataa agatatagag aataaagtta aatcagtttt
      361 atactttaat agaggtatta gtgatatcga tttaagactt tcaaagcaag cggaatatac
      421 ggttcatttt aaaaatggaa caaaaagagt tatcgatttg aaatcaggta tctacacagc
      481 tgacttaatc aatacaagtg acattaaagc tatcagtgtt aacgtagata ctaaaaagca
      541 acctaaagat aaagctaaag caaatgttca agtgccatat acaatcacag tgaacggcac
      601 aagccaaaac attttatcaa acctaacatt taataaaaat caaaatatta gttacaaaga
      661 tttagagggt aaagttaaat cagttttaga atcaaataga ggtattactg atgttgattt
      721 aagactttcg aagcaagcga aatatacagt taattttaaa aatggaacga agaaagttat
      781 agatttgaaa tcaggtattt acacagcgaa tttaatcaat tcaagtgata ttaaaagtat
//
LOCUS       DAMZKC010000348          825 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.353, whole genome shotgun sequence.
ACCESSION   DAMZKC010000348 DAMZKC010000000
VERSION     DAMZKC010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..825
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.353"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..374
                     /locus_tag="POB55_002808"
     CDS             <1..374
                     /locus_tag="POB55_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDI6501700.1"
                     /translation="SKYSNSPTKLFDSGFQKIHMKISEINKIEVESVIPILKDKNVVF
                     TGNFDTEKQDLMILTRKKGAYIRSDVTAKTDILVEGVQDDKYKDVNGLVSKQRKAREY
                     VGNGAKIQFLNEEDLINLIKE"
     gene            380..721
                     /locus_tag="POB55_002809"
     CDS             380..721
                     /locus_tag="POB55_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501701.1"
                     /translation="MIKKIFTKKHVFLVIEDENHNHSDAVFGKSILLSIYVGVNKKTN
                     SKSGKFIYLDRSKRIVRQSDITKIESANENDVDFYNLLKKEKEIVYSKNIVDKYNLAN
                     YIIYYEVSTKE"
BASE COUNT          358 a           95 c          117 g          255 t
ORIGIN      
        1 ttagcaaata ttctaactct ccaactaaac ttttcgattc aggatttcaa aaaattcata
       61 tgaaaatatc tgagattaat aaaatagaag tggaaagtgt aatccctatt ttaaaagata
      121 aaaatgttgt ttttacaggt aattttgaca ctgaaaaaca agatttaatg atattaacta
      181 gaaagaaagg agcttatatc agaagtgacg taactgcaaa aacagatatt ttagtcgaag
      241 gtgttcaaga tgataaatat aaagatgtga acggactagt ttcaaaacaa cgaaaagctc
      301 gagaatatgt tggaaatggt gcaaaaattc aatttttaaa tgaagaagac ttaataaatt
      361 taataaagga ataataacga tgatcaaaaa aatttttaca aaaaaacatg tattcttagt
      421 tatagaagat gaaaaccata atcacagtga tgctgttttt ggaaaaagta tattactttc
      481 aatttacgtc ggtgtgaata aaaagactaa ttctaaatca gggaaattta tataccttga
      541 cagatctaaa agaatcgtta gacaatctga tatcaccaaa atagaatcag ctaacgaaaa
      601 tgatgtagat ttttataatt tactgaagaa agaaaaggaa attgtttatt ccaaaaatat
      661 agtagataaa tacaatttag cgaactatat aatttactac gaagttagta ctaaagaata
      721 aaccaatcca ttatttcata atactaacct taaatttaca gaggttttaa ttatgaaaca
      781 tgaaaaaagc aatcttaact ttaagtctta tatttattac ctact
//
LOCUS       DAMZKC010000349          818 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.354, whole genome shotgun sequence.
ACCESSION   DAMZKC010000349 DAMZKC010000000
VERSION     DAMZKC010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..818
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.354"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
BASE COUNT          353 a          100 c          114 g          251 t
ORIGIN      
        1 gtttagtgaa tcgtctacat ttagttggac aaattctatg agaatagaca ttgttaaact
       61 aagagggtag gcgattttat tatgacaaga gactgaagag cttttagtaa ataatttaaa
      121 ttaaaataat taaactttat gaaaatggta agcttagaaa cgaaattata cgagaatatg
      181 atctaacatc attaactttc tcaaattaga taaaacaaaa ccaaaatact gagtcattca
      241 atcatcaaga cattttaaca agtgatgaaa aagagttaat aaaattacgc aaagaaaaac
      301 atcgattgat aatgtaaaat gatgatttaa agcaagcagt gctaattttg agacaaaaaa
      361 tgaagtcttt caacaaaatg cgtatcaata ttcagtatca gcaatgtgca aagttctaaa
      421 aatttcaaga agtacctatt atgattctaa aaaaggagaa tattacaact agatacaaaa
      481 agcctcaatc acaaccaagt aactaaaacg taaaaattaa ttagattaat tattatctgc
      541 ataacaaatg accatataca tcaactagag acagactatt taatcattta caatgtccta
      601 ttcgttttag accatgttca aatgaatagt gtttataaaa tgttgacact gtagtggcat
      661 attgtaaatt aaatattatt atgtgatcat tttgtttaaa tattagttat aagaagtgaa
      721 atattcattt taaaaggaga cttaaaactt gaaacgatca acgaatcaag aaaaatttct
      781 tgatacacta attagactta atactaagat tgaagaac
//
LOCUS       DAMZKC010000350          797 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.355, whole genome shotgun sequence.
ACCESSION   DAMZKC010000350 DAMZKC010000000
VERSION     DAMZKC010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..797
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.355"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            112..654
                     /locus_tag="POB55_002810"
     CDS             112..654
                     /locus_tag="POB55_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HDI6501702.1"
                     /translation="MVRESIPKEGENIKIQSYKHDGKIHRVWSETTILKGTDHVVIGG
                     NDHTLVTESDGRTWITREPAIVYFHSEYWFNVICMFREDGIYYYCNLSSPFVCDEEAL
                     KYIDYDLDIKVYPNGKYHLLDEDEYEQHMNQMNYPHDIDIILRRNVDILQQWIEQKKG
                     PFAPDFIKVWNERYKKIRQY"
BASE COUNT          301 a           92 c          149 g          255 t
ORIGIN      
        1 taattattaa gtagtaaaat cttatttcgt aaaaatatgt atttatataa tactatttct
       61 gaaatatgct ataatttata atggttaaat taacagaagg tggtgtggag tatggtcaga
      121 gaatccatac ctaaagaagg ggaaaacatt aaaattcaga gttataaaca tgatggtaag
      181 attcatcgcg tttggtctga aacaacaata ttaaagggta ctgatcatgt tgtcattggt
      241 ggcaatgatc atacgttagt aactgaaagt gacggaagaa cttggataac acgtgaaccg
      301 gcaattgttt actttcactc agaatattgg tttaatgtta tctgcatgtt tagagaagat
      361 ggaatttatt actattgcaa tttgtcatca ccatttgttt gtgatgaaga ggcattaaaa
      421 tatatagact atgatttaga tattaaagta tatccaaacg gaaagtatca tttattagat
      481 gaagatgaat atgaacaaca tatgaatcaa atgaattatc cgcatgatat tgatattatt
      541 ttaagacgca acgtggacat tcttcaacaa tggatagaac agaagaaggg cccattcgcg
      601 ccagatttta tcaaagtttg gaatgaacgt tacaaaaaaa ttagacagta ttaagcaaat
      661 aaagtgtaaa tactgattaa atcatttata ttgacttagt gttgtaaaga agtatgataa
      721 cttttgctgt cgtgtgggga cacagtatcc atagattaaa ttgaatagaa tataacatac
      781 gaataagaca acgtatt
//
LOCUS       DAMZKC010000351          783 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.356, whole genome shotgun sequence.
ACCESSION   DAMZKC010000351 DAMZKC010000000
VERSION     DAMZKC010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..783
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.356"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            40..762
                     /locus_tag="POB55_002811"
     CDS             40..762
                     /locus_tag="POB55_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020808033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6501703.1"
                     /translation="MLVLNNLQKKFTNSKMVLNNVNAKFRSGSINCIVGVNGAGKTTL
                     LNIISSILMPTKGDVYLNSESIFENSTLKKEIFYTPVNPFFYENLSAKDNLYLICSLY
                     NRKIDQITIEKTIKDVGLNIEDLNIPVYNFSSGMKQKLNFASMLLVDSNVLLLDEPFN
                     ALDHVAQKNFTRILKGLVSKEKIIIFTSHLPNTILELSENIYLLKDGCFTDKRKAQSF
                     NQTTLETWLLESQKEVIPYEEK"
BASE COUNT          311 a           80 c          128 g          264 t
ORIGIN      
        1 attagaagtt tatgtaacca taaattaagg aagtgtatca tgctagtttt aaataatctt
       61 caaaaaaaat ttactaattc aaaaatggtt ttgaataatg tgaatgctaa gtttagaagt
      121 gggagcatca attgcatagt gggagtaaat ggggcaggta aaacgacttt gttaaatatt
      181 attagtagta tattgatgcc aacaaaaggt gatgtatatt taaatagtga aagtattttt
      241 gaaaatagca cactaaaaaa agagattttt tacacaccag taaatccctt tttttatgaa
      301 aatttaagtg ctaaagataa tttgtactta atatgtagcc tatataatcg gaaaatagat
      361 caaataacta ttgagaaaac tattaaagat gtaggtttga atatagaaga tctaaatata
      421 cctgtatata atttttcaag tggtatgaag caaaaactaa attttgcttc tatgttatta
      481 gtagattcaa atgttttatt attagatgaa ccatttaatg cccttgacca tgttgctcaa
      541 aaaaatttca cacgaatttt aaagggatta gtttctaaag aaaagataat aattttcact
      601 agtcatttgc ctaatacaat tttagaactc tcagaaaata tttatttgtt aaaagatgga
      661 tgttttacag ataaacgaaa agcacagtca tttaatcaaa ccacgttaga aacatggttg
      721 ttagaaagtc aaaaagaggt gataccttat gaagaaaaat gatttgttag taatgagtgt
      781 agt
//
LOCUS       DAMZKC010000352          775 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.357, whole genome shotgun sequence.
ACCESSION   DAMZKC010000352 DAMZKC010000000
VERSION     DAMZKC010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.357"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            273..686
                     /locus_tag="POB55_002812"
     CDS             273..686
                     /locus_tag="POB55_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase DCC family
                     protein"
                     /protein_id="HDI6501704.1"
                     /translation="MPIVYYDGNCIYCYNYVIWLIQHDLPRNYQFATLKGEVGQQFFK
                     QHPEAANKNSVILQKGDCIYFESQAIIKLITALPNRTKLLGVGLWLVPKPVRNFGYRM
                     FANNRNRMWKTTWHQPNDYEKSFFLDDNAKVKLTD"
BASE COUNT          307 a           95 c          128 g          245 t
ORIGIN      
        1 tatgttacag aattaaaact ttatatgagt tttccagtgg tatgttgaat aagacgaatg
       61 accaaacgct aagttaataa taagaaataa gattacactg tagattgaga aatttaaagt
      121 aaataaaaaa tacattatac aataaatata aaactgaaaa ataaatttta tggcaatttc
      181 gtgtcgcgag gacttatcaa gtgacaacct tgtttaaatg tttttaaatt aggaaaaatg
      241 actataagta ctattaaagg agcgaataaa ttatgccaat cgtatattat gatggaaact
      301 gtatatattg ttataactat gtcatttggt taattcaaca tgacctacca agaaattatc
      361 aatttgccac gttaaaaggt gaagttggcc aacaattttt taaacaacat ccagaagcgg
      421 caaataaaaa tagtgtgatt ttacagaaag gtgattgtat atattttgaa tcgcaggcga
      481 ttattaaact aatcacagca ctacctaatc gaactaaatt attgggcgta ggtttatggc
      541 ttgtacctaa accagtccgt aatttcggtt atcgaatgtt tgctaataat agaaatagaa
      601 tgtggaaaac aacatggcac caaccaaatg attatgaaaa atcgttcttt ttagatgata
      661 atgcgaaagt aaaacttact gattgataaa acatacttgc taattgataa tggatatatt
      721 agatgatgaa ttaaaattta ggcatttaaa tagcggaaca ctttacgatt agatt
//
LOCUS       DAMZKC010000353          757 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.358, whole genome shotgun sequence.
ACCESSION   DAMZKC010000353 DAMZKC010000000
VERSION     DAMZKC010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..757
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.358"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(<1..223)
                     /locus_tag="POB55_002813"
     CDS             complement(<1..223)
                     /locus_tag="POB55_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501705.1"
                     /translation="MLGKYWIQLLIATVIISLISIKAFPLAIGALYLPVIFKVIKLQL
                     NLSKGLIDDVNAQTFIKSNQSGIVISVICC"
BASE COUNT          275 a          124 c          108 g          250 t
ORIGIN      
        1 aacaacaaat tacacttatt acaatacctg attgattact tttaatgaac gtttgcgcat
       61 taacatcatc gattaatcct tttgataaat tgagttgtaa ttttattact ttgaaaataa
      121 caggtaaata taatgcccca attgccaatg gaaaagcttt aattgatatt aaacttataa
      181 taactgttgc tatcaataat tgaatccagt attttcctaa cataaatata taaatctcct
      241 ctaatttcat tcttcaatag catatcataa tcttggcata ttaagaaacg cggtttaatg
      301 atttcattaa aaatattact gatagatgac ttctttcaat tatgtctgga gtaattaatt
      361 atcaattccg tttaaatggt gttttaatat ttaaaattga acttttgata tattactatg
      421 tctggtacac aaatcaatgt tttatgcttt acaaagttat attggcagta gttaactgca
      481 gtccacaaac atagaagctg gtggaaagtc agcttactat ttcatgcaag ttggcggagc
      541 tccaacatag tggaattgga ttcccaattt ctacagacat tgcaaattgg ggaaacgggc
      601 cacaacatag aaaaattgga tcctcaattt ctacaaacaa tgtaagtcgg ggaaacagcc
      661 ccaacactga aactggcaga aagtcagctt ctatgaatat aataaaaaag ctaggtaaca
      721 aaatgctacc taacttcata ttcaagataa tcaatcc
//
LOCUS       DAMZKC010000354          748 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.359, whole genome shotgun sequence.
ACCESSION   DAMZKC010000354 DAMZKC010000000
VERSION     DAMZKC010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.359"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..239
                     /locus_tag="POB55_002814"
     CDS             <1..239
                     /locus_tag="POB55_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HDI6501706.1"
                     /translation="SNNEKIILIPTFKNICLSLFAYILFGGLSTMALSMLVTSSPQNI
                     IEFLALIGMTACFFLLNMSSVLDKKIHVILKTNK"
     gene            296..412
                     /locus_tag="POB55_002815"
     CDS             296..412
                     /locus_tag="POB55_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501707.1"
                     /translation="MILNRGAILEQGNKTAKVIKSELITNYIMLATLQIKNN"
     gene            435..>748
                     /locus_tag="POB55_002816"
     CDS             435..>748
                     /locus_tag="POB55_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HDI6501708.1"
                     /translation="MLCESKIINKNPKYRIIKYNDEYLMIDIISTWISLFFPFINWFI
                     PKRYVKISREEFENLNIVKPAKKNVFWPVAGISTLFAVTLRKYTHLLDTQLDRKLVIA
                     IC"
BASE COUNT          285 a           94 c          109 g          260 t
ORIGIN      
        1 aaagtaataa cgaaaagatc atattaatac ctacatttaa aaatatttgt ttatccttat
       61 ttgcttatat attatttggt ggattgtcaa caatggctct gagtatgtta gtaacttcat
      121 cccctcaaaa tataatagaa tttcttgctt taattggcat gactgcatgc ttctttctac
      181 tgaatatgtc atcggttcta gataaaaaaa ttcatgttat tttaaaaaca aataagtagt
      241 aaaattgatt aacttaggta gtatcggata cttaaatgtt ggttcataaa aagcaatgat
      301 tttaaatcga ggagctatct tagaacaggg aaataaaaca gccaaagtta taaaaagtga
      361 attaataact aattatatta tgttagccac gcttcaaata aaaaataatt agaataaggt
      421 gggattgata atcaatgcta tgcgaatcta aaatcatcaa taaaaacccc aaatatagaa
      481 ttattaaata taatgatgag tacttaatga tcgatataat aagcacttgg attagtttat
      541 tttttccttt tattaattgg ttcatcccca aaagatacgt caaaattagt agagaagagt
      601 ttgaaaattt aaatattgtg aaacctgcta aaaagaatgt tttttggcca gttgcaggta
      661 tctctacttt attcgcagtt acattaagaa agtatacaca tttacttgac actcaacttg
      721 atagaaaatt agttattgct atatgttg
//
LOCUS       DAMZKC010000355          743 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.360, whole genome shotgun sequence.
ACCESSION   DAMZKC010000355 DAMZKC010000000
VERSION     DAMZKC010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.360"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..709
                     /locus_tag="POB55_002817"
     CDS             <1..709
                     /locus_tag="POB55_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6501709.1"
                     /translation="LFMLNVNIPNITNPARNNPSYLQNLKEGVLGIKNSLLLVVALVS
                     GVINFGLGLLYTWIPLKADHLGGAYYYGLLMALFSIGLIVGSLITPYIKKINLGYGKL
                     LILLNTISGLILILVNYIPRNFFIILFPVAFMTISITNISLASIQQKVIPEHLLARLT
                     TIITSISAISLPMGSFIGGILIKNLGIDYTLVIGGALFIIASIIFFISTPYRSLPKIE
                     KIEHYHIFGIQKIGNNH"
BASE COUNT          256 a          102 c           99 g          286 t
ORIGIN      
        1 tttatttatg ctgaatgtaa atattcctaa tataactaac cctgcaagaa ataatccttc
       61 ttatttacaa aatttaaaag aaggtgtgct aggaattaaa aattcactcc tactagttgt
      121 tgcattggta agtggtgtta taaattttgg actaggactt ctttatacat ggataccttt
      181 aaaggctgac catttaggtg gagcctatta ttacggttta ttaatggcac tattttcaat
      241 aggtttaatt gtaggttcat taatcacacc ttatataaaa aaaatcaact tgggatatgg
      301 aaaattatta atactcttaa atactatttc aggtttaata ttaattttag ttaactatat
      361 tccgcgaaat ttttttataa ttttgtttcc agtagctttt atgacaatta gtattaccaa
      421 tatatcctta gcttctattc aacaaaaggt tattccagaa catttgcttg ctagattaac
      481 tacaatcata acaagtattt cagcaatttc attaccgatg ggtagtttta ttggcggaat
      541 cctaataaaa aatcttggta ttgattatac tcttgtaatt ggtggagcat tatttattat
      601 tgcaagtatc attttcttta tatctacacc ttacagatca ttgcctaaaa ttgaaaaaat
      661 agagcattat catatattcg gaattcagaa aattggaaac aatcattaaa attatacatt
      721 tttattagtt caatatactt aaa
//
LOCUS       DAMZKC010000356          740 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.361, whole genome shotgun sequence.
ACCESSION   DAMZKC010000356 DAMZKC010000000
VERSION     DAMZKC010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..740
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.361"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            119..>740
                     /locus_tag="POB55_002818"
     CDS             119..>740
                     /locus_tag="POB55_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002494594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501710.1"
                     /translation="MNLRGHENRLKFLEKYDVTPISHLKLLEGQKKDGEGGRLTDSYY
                     CFSYSLKDNSKKVLGTFNCGYHIAEDLLKLSNQDKLPLFNPFKVINKGNQLQGVTNKG
                     NLDINRQRKQYNEVALQLSNAINLIIICYEGNIKEPLSTIKYETDKYYYSEPYDSKIK
                     AVNTIIGNLFDKKLVEKISEINVNQEIRKFDFSLLTNKIKKYEIQNN"
BASE COUNT          318 a           68 c          118 g          236 t
ORIGIN      
        1 ctaaaatacg ctgaaagtta tcataagtga tggtaaaaaa atgagtaagt agatgaagag
       61 tgaaaatcag attaattaat aataatgtat caaatttaaa taaagggggt ctttaagtat
      121 gaatttaaga ggtcatgaaa atagacttaa atttcttgag aaatatgatg tgacacctat
      181 atcacattta aaattattag aaggtcaaaa gaaagacggt gaaggcggta gactgacaga
      241 tagctattat tgtttttcat acagtttaaa agataattct aaaaaagttt taggtacgtt
      301 taattgtggt tatcatattg ctgaagattt actaaaatta tcaaatcaag acaaattacc
      361 tttatttaac ccgtttaaag taattaataa aggtaatcaa ttgcaaggcg taacgaataa
      421 aggtaattta gatattaata ggcaaagaaa acagtataat gaagtggctt tacagctttc
      481 aaacgctatt aatttaatca taatttgtta tgagggtaat attaaagaac cactttcaac
      541 gataaaatac gaaaccgata aatattatta tagtgaacca tatgatagta aaattaaagc
      601 agtaaatact attataggta atttgtttga taagaaatta gttgagaaga tatctgaaat
      661 taatgtaaat caggaaatta gaaaatttga tttttcatta ctcactaata aaattaaaaa
      721 atatgagata caaaataatt
//
LOCUS       DAMZKC010000357          738 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.362, whole genome shotgun sequence.
ACCESSION   DAMZKC010000357 DAMZKC010000000
VERSION     DAMZKC010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..738
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.362"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(<1..442)
                     /locus_tag="POB55_002819"
     CDS             complement(<1..442)
                     /locus_tag="POB55_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501711.1"
                     /translation="MNKLILGIYLYRIFSRAYFYLPFLLIYFLIQGYSIIQLEILMAS
                     YGIAAFLFSLYKEKCFKICNLKDSNKLVVSEIFKIIGLLLLLYQNQYLILVVAQILLG
                     LSYSMMAGVDTAIIKRNITNEKYVQNKSNSYMFLSLLISGIIGSY"
     gene            complement(435..>738)
                     /locus_tag="POB55_002820"
     CDS             complement(435..>738)
                     /locus_tag="POB55_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HDI6501712.1"
                     /translation="AIGCVQNKTMRRFISYINSPIAITSANISGTADDILITENEAIK
                     HMGENVRYMLRSQNKTNYKTSSTIIKVTDNKIELLREGDIKFEEIKERLGTGIIYE"
BASE COUNT          256 a          113 c           78 g          291 t
ORIGIN      
        1 gataactacc tataatccct gaaattaata atgataggaa catatagcta tttgacttat
       61 tttgtacgta tttctcattt gttatatttc ttttaattat tgcggtatca acacccgcca
      121 tcattgagta acttaaccct aataatattt gtgccactac taaaattaaa tattgatttt
      181 gatataataa caacaataaa ccgatgattt tgaatatttc actaacaact aatttattag
      241 aatcttttaa gttacaaatt ttaaaacact tctctttgta tagagagaat aaaaatgctg
      301 caatgccata agacgccatt aatatttcta attgtattat ggaataacct tgaatcaaaa
      361 agtaaattaa taaaaacggt aaataaaagt atgctcgtga aaaaattcgg tataaataaa
      421 tcccaagtat taacttattc ataaataata cctgtaccta gtctttcttt tatttcttca
      481 aactttatat ctccttctct taataattca attttattat ctgtcacttt aataatcgta
      541 ctagatgttt tatagttagt tttattttga cttcttagca tatatcttac attttcgccc
      601 atgtgcttaa ttgcttcatt ttcagttatt aaaatatcat cggcagttcc agatatattt
      661 gctgaagtaa ttgctatagg tgaattaata tatgaaataa atcttctcat cgttttattt
      721 tgtacacatc ctatagct
//
LOCUS       DAMZKC010000358          737 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.363, whole genome shotgun sequence.
ACCESSION   DAMZKC010000358 DAMZKC010000000
VERSION     DAMZKC010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..737
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.363"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            38..>737
                     /gene="splA"
                     /locus_tag="POB55_002821"
     CDS             38..>737
                     /gene="splA"
                     /locus_tag="POB55_002821"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplA"
                     /protein_id="HDI6501713.1"
                     /translation="MNENVMVKGLTALTILTSLGFAENISNQPHSIAKAEKNVKEITD
                     ATKEPYNSVVAFVGGTGVVVGKNTIVTNKHIAKSNDIFKNRVSAHHSSKGKGGGNYDV
                     KDIVEYPGKEDLAIVHVHETSTEGLNFNKNVSYTKFADGAKVKDRISVIGYPKGAQTK
                     YKMFESTGTINHISGTFMEFDAYAQPGNSGSPVLNSKHELIGILYAGSGKDESEKNFG
                     VYFTPQLKEFIQNNI"
BASE COUNT          283 a           95 c          143 g          216 t
ORIGIN      
        1 cattaactat aaaaataaat ttggaaggag gtaaaatatg aatgaaaatg taatggttaa
       61 aggtttaact gctttaacta ttttaacatc tcttggtttt gctgaaaata tttctaatca
      121 gcctcattca attgccaaag cagaaaagaa tgtcaaagaa attaccgatg caactaagga
      181 accatacaat tcagtggtag catttgtggg tggtactggt gtagttgttg gtaaaaatac
      241 aatcgtaact aacaaacata tcgctaaaag taatgatatt tttaaaaata gagtatcagc
      301 acatcattcg agtaagggta aaggcggagg aaactacgac gttaaagaca ttgtagaata
      361 tcccggaaaa gaagaccttg cgatagttca tgttcatgaa acaagtacag aaggtttgaa
      421 ttttaataag aacgttagtt atacaaaatt tgcagacgga gcaaaagtga aagatagaat
      481 ttctgttatt ggttatccaa agggtgcaca aacaaaatat aaaatgtttg aatcgacagg
      541 aacgattaac catatcagtg gaacgtttat ggaatttgat gcgtatgcac aaccaggtaa
      601 ttcaggatct cctgtattga attctaaaca tgaactgatt ggtattttat atgcaggtag
      661 tggaaaagat gaatctgaaa agaatttcgg tgtttatttc acaccacaat taaaagaatt
      721 tattcaaaat aatattg
//
LOCUS       DAMZKC010000359          736 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.364, whole genome shotgun sequence.
ACCESSION   DAMZKC010000359 DAMZKC010000000
VERSION     DAMZKC010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.364"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            6..596
                     /locus_tag="POB55_002822"
     CDS             6..596
                     /locus_tag="POB55_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HDI6501714.1"
                     /translation="MKVNPNNIELIISAVKEEQYPETELSEVALSGRSNVGKSTFINS
                     MIGRKNMARTSQQPGKTQTLNFYNIDEQLIFVDVPGYGYAKVSKTQREKFGKMIEEYI
                     TKRENLQLVIQLVDLRHDPTQDDILMYNYLKHFDIPTLVICTKEDKIPKGKVQKHIKN
                     IKTQLDMDPDDTIVSYSSIQNNKQQQIWNLIEPYIS"
BASE COUNT          314 a           93 c          107 g          222 t
ORIGIN      
        1 ataaaatgaa agttaatcct aataatatag aattaatcat tagtgcagta aaagaagaac
       61 aatatccaga aacagaattg tctgaagttg cactgagcgg tcgatctaat gtaggtaagt
      121 ctacatttat caatagtatg attggcagaa aaaatatggc acgtacatca cagcaacccg
      181 gcaaaacgca aacgttaaat ttttataata tagatgaaca acttattttt gtggatgttc
      241 cagggtatgg atatgctaaa gtaagtaaaa cacaacgtga aaaatttggg aaaatgattg
      301 aggaatatat aactaagaga gagaatttgc aattagttat tcaattagtt gatttaagac
      361 atgatccaac acaagatgat atcttaatgt acaattattt gaaacatttt gatattccta
      421 ctttagttat atgcactaaa gaagacaaaa ttccaaaagg taaagttcaa aagcatatta
      481 aaaatattaa gacacaatta gatatggacc cagacgatac aattgtaagt tattcatcaa
      541 ttcaaaataa taaacaacaa caaatatgga atttaattga accgtatatt tcatagtttt
      601 tgtacgacaa aacttataca aaaattttaa aaataatgta agcacgaaac ttttaattag
      661 tacacaattg ataacatttt tcaacgttca tcattttgtc aaaaactcaa aagtaaatta
      721 gaaagattat aattta
//
LOCUS       DAMZKC010000360          736 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.365, whole genome shotgun sequence.
ACCESSION   DAMZKC010000360 DAMZKC010000000
VERSION     DAMZKC010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.365"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
BASE COUNT          180 a          169 c          102 g          285 t
ORIGIN      
        1 tataataatt aatttccttt ttgtatttcg atttaaaaaa tattagacat aaaacctaaa
       61 aacagcagta agatgattta tgattaaaaa ctatcttact gctgttcact ttttataata
      121 cttctgaatg tcttcactta tacttctagt cacagattta aacaatcaaa agtgcacatt
      181 attaaaatat catttccaaa actcaatgcg gctcatcgca tccatttctt gcctagcaac
      241 gttctactct agcggaacgt aagttggcta ccatcgtcgc taaagacctt tcttgacttg
      301 tgacaattgc ttgcttcttt cctctttttc ggctctcact tactcattta gctctactaa
      361 actcgttgcg ctcttttctc gtttcgtcag attcaaacgt tttcacttcg ccaagccatt
      421 tttctttgtg tttgcttttt attttgatgt tttagacata aaaaaagaga cctcacggtc
      481 tcaatgcggc tcatcgcatc catttttgcc tggcaacgtt ctactctagc ggaacgtaag
      541 ttggatacca tcgtcgctaa gaacctttct tgacttgtga caatcgcttg cttctttcct
      601 ctccttcggc tctcgcttac tcatttagct ctactaaact cgttgcgctc ttttctcgtt
      661 tcgtcagatt caaacgtttt cacttcgcca agccattttt ctttgtgttt gctttttatt
      721 ttgatgtttt agacat
//
LOCUS       DAMZKC010000361          725 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.366, whole genome shotgun sequence.
ACCESSION   DAMZKC010000361 DAMZKC010000000
VERSION     DAMZKC010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.366"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            500..718
                     /locus_tag="POB55_002823"
     CDS             500..718
                     /locus_tag="POB55_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501715.1"
                     /translation="MTKIMNQFKEIYNTIEKLLNDKSISNYRINQDTGVSYGGISELR
                     SGKRKVNNLTLETAEKLYNYQKQLEIMI"
BASE COUNT          314 a           73 c          108 g          230 t
ORIGIN      
        1 agttctttct tttttacaag atgtatatgt ataaatggaa atgtttatta cattacataa
       61 aaacctttag gtgctcttag atggatgtta atcataaaaa aagagatttt gaaaaatata
      121 ggctattata atttagaatt acaaattata aatcttgcca gtggatatgg gagttcaaca
      181 tgtcacttac cccaatttgt ggaatgttaa ataactataa agaggaaagg agaaaaaatg
      241 ataagaatgg ttaaagaaaa agaacgaata gcaaaattaa atttcgaata gctgtgacga
      301 gcttaataat tcaagtactt tattttatca aatcgttctt ttaaaaaggt aaagggcaaa
      361 agacctttac cacattaaat agggaggtgg tatttatgac aagagaatta aggaaaaaat
      421 taactctcta tcttaacatt gcaactttaa tattatttat tattaattta actagaaaaa
      481 aataaactat tctttcaatt tgacaaaaat tatgaaccag tttaaagaaa tttataatac
      541 aatagaaaaa ttactgaatg ataaatcaat atctaattat agaattaatc aagacactgg
      601 tgtttcttat ggtggtataa gtgaattaag aagcgggaaa agaaaagtga ataatttaac
      661 tttagaaaca gcggaaaaac tctataatta ccaaaaacaa ttagaaataa tgatttaaga
      721 ttaaa
//
LOCUS       DAMZKC010000362          715 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.367, whole genome shotgun sequence.
ACCESSION   DAMZKC010000362 DAMZKC010000000
VERSION     DAMZKC010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..715
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.367"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(231..443)
                     /locus_tag="POB55_002824"
     CDS             complement(231..443)
                     /locus_tag="POB55_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501716.1"
                     /translation="MEKNESNITDVTQNEEQLDNSDEQSQQNEKTFSQEEVSQLIKER
                     IARERKKSDERIKDAVQEAEKLAKMK"
     gene            complement(410..538)
                     /locus_tag="POB55_002825"
     CDS             complement(410..538)
                     /locus_tag="POB55_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501717.1"
                     /translation="MKVIFYCPKRADDILKASMEYQSTDYKRRYVSWRKMKVILLT"
BASE COUNT          194 a          129 c           95 g          297 t
ORIGIN      
        1 tactacacta tagttagtaa caatagtcgt tgtttggata gatttatttc caataaacat
       61 ctttaaacca ttcaatgaaa atcctgattg tccaggatgg ttatgtaaca taacaattga
      121 attcggttta ctgttaaata ataattcggt tgcttgttac cctggcacaa aagatacact
      181 atcttgatcg ccatatactt ttgtaacttt agcacctttt aataaataag ctacttcatt
      241 ttagctaact tctcagcttc ttgaaccgca tctttaatac gttcatctga ttttttgcgt
      301 tctctagcta ttcgctcttt aatcaattgt gatacttctt cttgagaaaa tgttttctca
      361 ttctgttgtg attgttcatc actgttgtct agttgctctt cgttctgagt tacgtcagta
      421 atattacttt catttttctc catgagacat acctccgttt atagtctgtc gactgatatt
      481 ccatacttgc ttttaaaatg tcatcagcac gttttggaca ataaaaaata accttcacat
      541 tggaaggtta aaaaagtgta taaaaatagc accactttct ctttatctgt gtagaaagga
      601 tgctattttt ctaatttttg ttctttattc ttattttcta attctattgc tttcttgtat
      661 tctttatcca ataattcttg tgactgttta ttagttattt caccatctaa ctgtg
//
LOCUS       DAMZKC010000363          713 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.368, whole genome shotgun sequence.
ACCESSION   DAMZKC010000363 DAMZKC010000000
VERSION     DAMZKC010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..713
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.368"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..512
                     /locus_tag="POB55_002826"
     CDS             <1..512
                     /locus_tag="POB55_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HDI6501718.1"
                     /translation="NEIIRLTIGSKSFKICYSQNYLKEQYKGIGILYKNQQWYFVVVN
                     IASKLVKLLNISKIKEVYEMGETKECNEITMQNFQQFMVENENTIDIQIRSNIIGLNI
                     LKGYLWSDYMIENINEETYLFKSKVNMQDINFIAKLIVTCGANVKVEFPISLKKAIKT
                     ELTKIINLY"
BASE COUNT          300 a           76 c          108 g          229 t
ORIGIN      
        1 ttaatgaaat cattagatta acaattggta gtaagtcatt taaaatctgt tattcacaaa
       61 actatttgaa agaacaatat aaaggtattg gtatcttata taaaaatcaa caatggtact
      121 tcgttgttgt taatatagca tcaaaattag taaagttgtt aaacatttct aaaataaaag
      181 aagtatatga aatgggagaa actaaagaat gtaatgaaat aactatgcag aattttcaac
      241 agtttatggt tgaaaacgaa aatacgattg atatacaaat tagaagtaat attattggat
      301 taaatatctt gaaaggatac ttgtggagtg actatatgat tgaaaatatt aacgaagaga
      361 catatttgtt taaatcaaaa gtgaacatgc aagatataaa ttttatagct aaattaatag
      421 tcacatgtgg tgccaatgtc aaagtagaat ttcctattag tttgaaaaaa gctattaaaa
      481 ctgaactaac caaaataata aacttatact aatggtaatt taattatgat tgcaataaca
      541 aagaaaagat aatcttaaac atattaaatt gatccgtagt ttaaaaaccg catcactaac
      601 cgagacgcag aggcgtatca taagtaatga ggttcatgat ttttgacata gttagcctcc
      661 gcagtctttc aagtaaataa tatcgaaata ttctttatag tgaatattta tag
//
LOCUS       DAMZKC010000364          712 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.369, whole genome shotgun sequence.
ACCESSION   DAMZKC010000364 DAMZKC010000000
VERSION     DAMZKC010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.369"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
BASE COUNT          270 a          113 c           67 g          262 t
ORIGIN      
        1 atacaatatc ttttccattt acatcttatg tttaaagatc aatagtacta caactattta
       61 aggatagata tacattctaa gtttttaaat acattcttca gttatatttg tatattccaa
      121 atgtttatta atagcatttc tgatctcaga aatgccatta aggacaacgc cctctttcat
      181 aactatataa gtcaagaaac caagaaattc caatcatctt taaacctttc aatagcacat
      241 ttcctaaaac ataaaacaat aacaatgaac gatcaaaatt acttacgtgc gtatttaatt
      301 atagatttgt caatttcaag tgatttagcc ataactataa attttcctat tacaaaaaaa
      361 ggccagctat gtttcgcatt gttaagaggc gttagctgac ttctgataaa ttaatattaa
      421 tctaagtgtt attaattttc aaatataatt ccattaatat ttgaaataaa tcatcaattc
      481 tataatttaa aatacatcta tcctcaaatc tcactatatc ttcaataaat ataggtacta
      541 atattggaat atcaacaatt aaaagtaaaa agctatttac aatctattcg tcttattata
      601 tatatgtctg acattatgac ttattcacat aagtataata gatttcttcc gagacatact
      661 ctgttgattg gagaacatgt tcatactcat tttcaattac aaaataattt ac
//
LOCUS       DAMZKC010000365          712 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.370, whole genome shotgun sequence.
ACCESSION   DAMZKC010000365 DAMZKC010000000
VERSION     DAMZKC010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.370"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            240..>712
                     /locus_tag="POB55_002827"
     CDS             240..>712
                     /locus_tag="POB55_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501719.1"
                     /translation="MILSNIIIYAIYDVIRKYWSGTGDRKLMDIYISQNNRYLKPLAK
                     EVFRNELLRWNEENINKTSINIQTDEKMIITFIANKFRSFYSSLNDNLDYEHIFSRKL
                     YKLHKGNAIIPAGSLGNIMLLDSGINRRKKEKFLYSAIDIDKASITGTIENQYVN"
BASE COUNT          296 a           77 c          106 g          233 t
ORIGIN      
        1 tagaaatctt gtttgaaaaa attgtgaaga tatataaaat aataaataac aagtttaaag
       61 aagctttctc tatggtagta tcgtcaagag aagataaata tgaaactaaa tcttttacta
      121 gattacaaat aatgtcattc ttttcatctt tatggatctc atcgtatagt attgaattag
      181 atgaaaataa aaacacattt tctattaaac aaactaaatc tttgaaaaaa aatcaacaag
      241 tgattttgtc taatatcata atttatgcaa tttatgatgt gataagaaag tactggtcag
      301 gaacaggtga tcgaaaatta atggatatat acatatctca aaacaataga tatttaaagc
      361 cgttagcaaa agaagtgttt agaaatgagc tacttagatg gaatgaagag aatataaata
      421 agacaagtat taatattcaa acggatgaga agatgatcat tacttttatt gctaataaat
      481 ttagaagctt ctattcatct ttgaatgata atttagatta tgaacatatt ttttcaagaa
      541 aattatacaa gctgcataaa ggtaatgcaa taatccctgc tggatcttta ggtaacatca
      601 tgttacttga ctctggtatt aatagaagaa aaaaagaaaa atttctttat tcagcaatag
      661 atattgataa ggcatctatt acgggaacta tagaaaatca gtatgttaac aa
//
LOCUS       DAMZKC010000366          695 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.371, whole genome shotgun sequence.
ACCESSION   DAMZKC010000366 DAMZKC010000000
VERSION     DAMZKC010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.371"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            35..385
                     /gene="scn"
                     /locus_tag="POB55_002828"
     CDS             35..385
                     /gene="scn"
                     /locus_tag="POB55_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement inhibitor SCIN-A"
                     /protein_id="HDI6501720.1"
                     /translation="MKIRKSILAGTLAIVLASPLVTNLDKNEAQASTSLPTSNEYQNE
                     KLANELKSLLDELNVNELATGSLNTYYKRTIKISGQKAMYALKSKDFKKMSEAKYQLQ
                     KIYNEIDEALKSKY"
     gene            complement(438..677)
                     /locus_tag="POB55_002829"
     CDS             complement(438..677)
                     /locus_tag="POB55_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501721.1"
                     /translation="MYSLIVYFICIINFFIMAAPFLMTNADYVWTPMTTVTLFILSLI
                     IFLIFIKTKDVVHLTIFILNLLFSILYCLPILFYL"
BASE COUNT          318 a           87 c           99 g          191 t
ORIGIN      
        1 ataaattagt actaattact aaggagaata aaaaatgaaa attagaaaat ctatacttgc
       61 gggaacttta gcaatcgttt tagcatcacc actagtaact aatctagata aaaatgaggc
      121 acaagctagc acaagcttgc caacatcgaa tgaatatcaa aacgaaaagt tagctaatga
      181 attaaaatcg ttattagatg aactaaatgt taatgaatta gctactggaa gtttaaacac
      241 ttattataag cgaactataa aaatttcagg tcaaaaagca atgtatgctc ttaagtcaaa
      301 agactttaag aaaatgtcag aagcaaaata tcaacttcaa aagatttata acgaaattga
      361 cgaagcacta aaaagtaaat attaaaaaaa ccacccgtaa aagggtggtt ttaattttct
      421 agataatata aaagtgttca taaataaaac agtataggca aacaataaag tattgaaaaa
      481 agtaagttta atatgaaaat tgttaaatga acgacatctt ttgtttttat aaatatcaag
      541 aaaataatca aactcaaaat aaataacgta actgtagtca taggcgtcca tacataatca
      601 gcattagtca ttaagaatgg tgcagccatt atgaaaaaat ttataatgca gatgaaatag
      661 acaattagac tataaattag gtaaataaca ataca
//
LOCUS       DAMZKC010000367          695 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.372, whole genome shotgun sequence.
ACCESSION   DAMZKC010000367 DAMZKC010000000
VERSION     DAMZKC010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.372"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(<1..363)
                     /locus_tag="POB55_002830"
     CDS             complement(<1..363)
                     /locus_tag="POB55_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HDI6501722.1"
                     /translation="METLLCESKVINKNPKYRIIKYDSEYLMIDLASNWIVFFFPFIN
                     WLIPKTYVKITKNDYEKLNIVKPVKNKSIGWTIFAGIVLLGGTVRRNTYLFDFQLEEL
                     IVWSSCFIGFLEIIFFYCY"
     gene            complement(494..>695)
                     /locus_tag="POB55_002831"
     CDS             complement(494..>695)
                     /locus_tag="POB55_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HDI6501723.1"
                     /translation="TLKNFCLTLFRYAFFILWTVIFSYALLSMSYQNIIVYFAWITAI
                     MGFFLVNIALIIDKNIHVILKN"
BASE COUNT          263 a          106 c           73 g          253 t
ORIGIN      
        1 ataacaataa aaaaatataa tctctaaaaa cccaatgaaa cagctgctcc aaacaattag
       61 ttcttctaat tgaaaatcaa ataaataagt atttcttctt acagtaccac caagtaacac
      121 aatacccgcg aatatggtcc atcctatcga tttattttta actggtttga caatatttaa
      181 tttttcataa tcattcttag tgattttgac atatgttttc ggtatgagcc agttaataaa
      241 tggaaagaag aagacaatcc aattacttgc caaatcaatc attaaatatt cactatcgta
      301 tttgattatt cgatatttag ggtttttatt aataacttta gattcgcaaa gcaatgtctc
      361 cacatccctt taattttatg tgtaatacat ttttcgatac ttcaaaagac attcaaatac
      421 tatcaagtta ctgtcatcaa aggttttatt aactgatatt ttcatatttt taatctgaat
      481 ttagtacttt cacttaattt ttaagtatga catgaatgtt tttatctata attaaagcta
      541 tattcactag aaaaaaaccc attattgctg taatccaagc aaaatatact attatgtttt
      601 gataactcat tgataataga gcatatgaga aaatcactgt ccataaaata aaaaaagcat
      661 atctaaacaa tgttaaacaa aaatttttta gtgtt
//
LOCUS       DAMZKC010000368          695 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.373, whole genome shotgun sequence.
ACCESSION   DAMZKC010000368 DAMZKC010000000
VERSION     DAMZKC010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.373"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..247
                     /locus_tag="POB55_002832"
     CDS             <1..247
                     /locus_tag="POB55_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501724.1"
                     /translation="SSGLTRPQTINKLSSSSNLRVMLNYAETLYFIFNIAFLLMGGYL
                     YTIGTIQYLILFISLLIFIYLIIIFYFTRREQHENKN"
     gene            231..>695
                     /locus_tag="POB55_002833"
     CDS             231..>695
                     /locus_tag="POB55_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HDI6501725.1"
                     /translation="MKIKTEFKGNNIPYEYAAGADVSDSINGNPIKSFPFEVIELPEG
                     TKYLAWSLIDYDAIPVCGFAWIHWSVANVSVSGNSISIKADLSRTKGDYVQGKNSFTS
                     GLLAEDFSEIENHYVGPTPPDQDHQYELTVYALDHSLNLKNGFYLNEFLKEVN"
BASE COUNT          242 a           86 c          121 g          246 t
ORIGIN      
        1 ttcttcaggg ttaactcgtc cacaaactat aaataaactt tctagcagta gtaacttaag
       61 agtgatgctt aattatgcag aaacgttata ttttattttt aatatcgcat ttttacttat
      121 gggtggttac ttatatacaa taggaactat tcaatactta atattattta tttcgttatt
      181 aatttttata tatttaataa taatatttta ttttacaagg agagagcaac atgaaaataa
      241 aaactgaatt taaagggaac aatataccat atgaatacgc agcaggtgca gatgtgagtg
      301 attctattaa cgggaatcca attaagtcat ttccatttga agtaattgaa ttaccggaag
      361 ggactaaata tcttgcttgg tctttaattg actatgatgc aattcctgta tgtggctttg
      421 cttggattca ttggagtgta gctaatgtaa gtgttagtgg caattcaatt tctataaaag
      481 cagatttatc aagaacaaag ggtgactatg tacaaggtaa aaatagcttt actagtgggt
      541 tgttggctga agatttttca gaaatagaaa atcactatgt aggacctaca ccacctgatc
      601 aagatcatca atatgaatta acagtttatg cgttagatca ttctttaaat ttgaagaatg
      661 ggttctactt gaatgaattt ttaaaagaag taaat
//
LOCUS       DAMZKC010000369          694 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.374, whole genome shotgun sequence.
ACCESSION   DAMZKC010000369 DAMZKC010000000
VERSION     DAMZKC010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.374"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..181
                     /locus_tag="POB55_002834"
     CDS             <1..181
                     /locus_tag="POB55_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HDI6501726.1"
                     /translation="VKYIAFGNDHNDIVMLQHASSGYIIGPSEAYTHAILKLDKIKHI
                     NNNAQAICKVLKSYK"
     gene            complement(230..>694)
                     /locus_tag="POB55_002835"
     CDS             complement(230..>694)
                     /locus_tag="POB55_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HDI6501727.1"
                     /translation="LDIASIRSIERGFNYYQSECVINLKSFSETQHEAEVKGSGNKVY
                     RCYIDMEHPRKSKCNCPHADGRRVICKHMIALLFTASPEAANKHIMMLNEVEEDYQLR
                     RNMWIDSLKEMINDMSEEELRDAYLNMLIEHGEMAELFGLDEEEEMFEDEFY"
BASE COUNT          230 a          136 c           86 g          242 t
ORIGIN      
        1 tgttaaatat atagcatttg gtaatgacca caatgatatt gtcatgttac aacatgctag
       61 tagtggctat attataggac catcagaagc atacacacac gcaatattga aacttgataa
      121 aatcaaacac atcaataata atgcacaagc tatttgcaaa gtcttaaaat catataaata
      181 aaaaacaccc ctatcaaatg ataatcatta tcaatcgata ggggctattt taataaaatt
      241 cgtcctcgaa catttcttcc tcttcatcta atccaaataa ttctgccatt tctccatgtt
      301 caattaacat gtttaaatat gcatcgcgga gttcttcttc actcatatca ttaatcattt
      361 ctttaagact atcaatccac atatttctgc gtaattgata gtcttcttca acttcgttta
      421 acatcattat atgtttattt gctgcttctg gactagctgt aaagagtaat gcaatcatat
      481 gtttacatat cactcgtctt ccatcagcat gaggacaatt acatttggat tttctaggat
      541 gttccatatc aatataacaa cgatatactt tgttgccact gccctttact tcagcctcat
      601 gctgcgtttc tgaaaatgat tttaagttaa tgacgcattc actttgataa taattaaagc
      661 ctctttctat agaacgaata cttgcaatat caag
//
LOCUS       DAMZKC010000370          686 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.375, whole genome shotgun sequence.
ACCESSION   DAMZKC010000370 DAMZKC010000000
VERSION     DAMZKC010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.375"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(24..242)
                     /locus_tag="POB55_002836"
     CDS             complement(24..242)
                     /locus_tag="POB55_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501728.1"
                     /translation="MSEWHINNQSTKPFLIQQAEKQLSIVKPLPNGSFQILTEINLEN
                     GNISNIDHSLLVSVDPKALEISIFDAVN"
     gene            complement(304..588)
                     /locus_tag="POB55_002837"
     CDS             complement(304..588)
                     /locus_tag="POB55_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501729.1"
                     /translation="MNELTKEQKYTIAKFYKLYIERSNNGETETVANFFGDAKDAREN
                     YFCDRDYQDFLTNCQILIQNKYLTGEVLDDNIYNISILNKTFIEFEQNFG"
BASE COUNT          227 a           98 c           98 g          263 t
ORIGIN      
        1 ataattaaat aaaaatattg cgtttaattt acagcgtcaa atatacttat ttctaatgct
       61 ttggggtcta ctgaaacaag taaagaatga tcgatgttac taatattgcc attctccaaa
      121 tttatttctg tgagtatttg gaagctacca ttaggcaacg gtttaacaat agacaattgc
      181 ttttccgctt gttgtattaa aaaaggtttt gtagattgat tattaatatg ccattcactc
      241 atgtatgttt ttcactcctg ctttaaaata gggttagaaa gtttatagtt gagacattca
      301 tgttcaacca aaattttgtt cgaattcaat aaatgtcttg tttaaaatag aaatattgta
      361 aatgttatcg tccaaaactt caccagttaa gtatttgttt tgaattaaaa tttggcagtt
      421 agttaagaag tcttgataat cacgatcaca aaaatagttt tcacgtgcat ctttagcatc
      481 gccaaaaaag ttagcgactg tttctgtttc tccattattc gaacgttcaa tatataattt
      541 gtaaaattta gctattgtat acttttgttc tttagttagt tcattcaaaa tattgggcct
      601 cctgaaatat catttgtaat ctatacccaa tttattgcaa aacacaaact aatttaacta
      661 tttgatgaaa ctgtgttaat aagctt
//
LOCUS       DAMZKC010000371          664 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.376, whole genome shotgun sequence.
ACCESSION   DAMZKC010000371 DAMZKC010000000
VERSION     DAMZKC010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.376"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..>664
                     /locus_tag="POB55_002838"
     CDS             <1..>664
                     /locus_tag="POB55_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001623710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MAP domain-containing protein"
                     /protein_id="HDI6501730.1"
                     /translation="NKAKRNYQVPYSINLNGTSTNILSNLSFSNKPWTNYKNLTSQIK
                     SVLKHDRGISEQDLKYAKKAYYTVYFKNGGKRILQLNSKNYTANLVHAKDVKRIEITV
                     KTGTKAKADRYVPYTIAVNGTSTPILSKLKISNKQLISYKYLNDKVKSVLKSERGISD
                     LDLKFAKQAKYTVYFKNGKKQVVNLKSDIFTPNLFSAKDIKKIDIDVKTGSKAKADSY
                     VP"
BASE COUNT          299 a           86 c           93 g          186 t
ORIGIN      
        1 aaaataaagc taaaagaaac tatcaagttc catattcaat taatctaaat ggtacatcta
       61 caaacatttt atcgaatctt tcattttcaa ataaaccttg gacaaattac aaaaatttaa
      121 ctagtcaaat aaaatcagta ctgaagcatg atagaggtat tagtgaacaa gatttaaaat
      181 atgctaagaa agcttattat actgtttatt ttaaaaatgg tggtaaaaga atcttacagt
      241 taaattcaaa aaattacaca gcaaacttag ttcatgcgaa agatgttaag agaattgaaa
      301 ttactgttaa aacaggaact aaagcgaaag cagacagata tgtaccatac acaattgcag
      361 taaatggcac atcaacacca attttatcaa aactaaaaat ttcgaataaa caattaatta
      421 gttacaaata tttaaacgac aaagtgaaat ctgtattaaa aagtgaaaga ggtatcagtg
      481 atcttgactt aaaatttgcg aaacaagcaa aatatacagt atatttcaaa aatggaaaga
      541 aacaagtagt gaatttaaaa tcagacatct ttacacctaa tttatttagt gccaaagata
      601 ttaaaaagat agatattgat gttaaaacag ggtcaaaagc gaaagcggat agctatgtac
      661 cata
//
LOCUS       DAMZKC010000372          643 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.377, whole genome shotgun sequence.
ACCESSION   DAMZKC010000372 DAMZKC010000000
VERSION     DAMZKC010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.377"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..476
                     /locus_tag="POB55_002839"
     CDS             <1..476
                     /locus_tag="POB55_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1829 domain-containing protein"
                     /protein_id="HDI6501731.1"
                     /translation="FVQTNFKNFNKSKHNLLQCLIFVNDMYLLSNPKSQNIFTEDVAN
                     KLDEHNIYYGRDLPIIGSSGVVHNFDFFISAKKNQKEKFINAISNPNNSMIIKSKITD
                     AMQAKKIKRHRQNEFIFILNDSKKEINEHNKNLLHENYISTIDYSELDEKIGLLI"
BASE COUNT          265 a           73 c           91 g          214 t
ORIGIN      
        1 tttttgttca aactaacttt aaaaatttta ataaatcgaa acataattta ttacagtgcc
       61 ttatatttgt taatgatatg tacttacttt ctaatcctaa gtcacagaac atatttacag
      121 aagatgttgc aaacaaattg gatgaacata acatttatta cggaagagac ttacctatta
      181 taggaagcag tggtgttgtt cataatttcg acttttttat tagcgctaag aaaaatcaaa
      241 aagaaaaatt tatcaatgct atttctaacc ctaataattc tatgattatt aagtcgaaaa
      301 taacggatgc tatgcaagca aaaaaaataa aaagacacag gcaaaatgag tttattttta
      361 ttttaaatga ctcaaaaaaa gaaataaatg aacataataa aaatcttctt catgaaaact
      421 atattagtac aatagattat agcgaattag atgaaaagat aggtttattg atttaatatt
      481 tatagacgtg ataatatcaa tgtttatatt aaatgaaacg aatttgaaaa attcgaaaca
      541 gcttaagata gcaaattgaa tggccttatt gataatgcag aatcgtctgc acttagttga
      601 acaaattcta tgagaataga tattgttaaa ctatgtggat agg
//
LOCUS       DAMZKC010000373          628 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.378, whole genome shotgun sequence.
ACCESSION   DAMZKC010000373 DAMZKC010000000
VERSION     DAMZKC010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.378"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            269..>628
                     /locus_tag="POB55_002840"
     CDS             269..>628
                     /locus_tag="POB55_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDI6501732.1"
                     /translation="MEQLPQVGFMKSFVLFWKNYVNFKGRSRRSEYWYMALWHLILMV
                     PAVCVLIINLFLFFISIAADNETLIVMSILMIILTSVYTFVYSLATFIPNLALCVRRF
                     HDISRTMLFPMIMTVFSI"
BASE COUNT          205 a           74 c          117 g          232 t
ORIGIN      
        1 attttatttg cagagattga gactctttta aacgtgaaca ttgcagtgtg acatattttt
       61 agtatagtta acgaagcgtt gactttcaaa tgagtctcca tatagatgtg tacttgtagc
      121 gggttttcat tttactagtg atgaaatata aagttcattt tccaaagtga ttgacgattg
      181 aaatgaagtg gcttgataat gaatcttgta aattataacg gtaacaataa ttaaaaatat
      241 aaatatgatt aacgaaaggg aggttacaat ggaacaatta cctcaagtag gttttatgaa
      301 atcattcgta ttattttgga aaaattatgt aaattttaag gggagatcaa gacgtagtga
      361 atattggtat atggcactat ggcatttgat acttatggtt ccagctgtgt gtgtgctaat
      421 aataaaccta tttctattct ttatttctat agcggcagat aacgaaacat taatagttat
      481 gagtatattg atgattatcc ttacatctgt gtatactttt gtctatagtt tggcaacgtt
      541 tattccaaat ttagcgttgt gcgttcgaag atttcacgat atatcaagaa caatgttatt
      601 tccaatgatt atgacagtat tcagtatt
//
LOCUS       DAMZKC010000374          621 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.379, whole genome shotgun sequence.
ACCESSION   DAMZKC010000374 DAMZKC010000000
VERSION     DAMZKC010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..621
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.379"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(152..601)
                     /gene="ndk"
                     /locus_tag="POB55_002841"
     CDS             complement(152..601)
                     /gene="ndk"
                     /locus_tag="POB55_002841"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HDI6501733.1"
                     /translation="MERTFLMIKPDAVQRNLIGEVISRIERKGLKLVGGKLMQVPMEL
                     AETHYGEHQGKPFYNDLISFITSAPVFAMVVEGEDAVNVSRHIIGSTNPSEASPGSIR
                     GDLGLTVGRNIIHGSDSLDSAEREINLWFNENEITSYASPRDAWLYE"
BASE COUNT          206 a          118 c           80 g          217 t
ORIGIN      
        1 aatacgtgtt accaaaaaaa ctataaaatt tttcaagtaa tttactacaa cataaaaaac
       61 gggccttgtt atgtataatc ataatgacta accacataac aaaacccttt ctacctttat
      121 aaataaatcg taaaggttta cagtttaaat tttattcata taaccatgca tcacgtggtg
      181 aagcatagct agtaatttca ttttcattaa accatagatt aatttcacgt tcagcagaat
      241 ctaatgaatc tgaaccgtga atgatatttc taccaacagt taaacctaaa tcacctctaa
      301 ttgatcctgg tgaagcttct gaaggattgg tgctgccaat aatatgtcta gatacattaa
      361 ctgcatcttc accttcaact accattgcga acactggtgc tgatgtaata aatgaaatta
      421 aatcattata aaatggttta ccttggtgtt caccataatg tgtttcagca agttccattg
      481 gtacttgcat taatttacca ccgacaagtt ttagtccttt tctttcaatt cttgaaatta
      541 cttcaccaat tagatttctt tgtactgcat ctggtttaat cattaaaaat gtacgttcca
      601 ctttatgtat ccccctgtgt t
//
LOCUS       DAMZKC010000375          619 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.380, whole genome shotgun sequence.
ACCESSION   DAMZKC010000375 DAMZKC010000000
VERSION     DAMZKC010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.380"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            1..619
                     /locus_tag="POB55_002842"
                     /pseudo
     CDS             1..619
                     /locus_tag="POB55_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500432.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          243 a           75 c           91 g          210 t
ORIGIN      
        1 aaattagaac ttcaaactta tatgagtaaa atcgaaaaag tattattaaa atatcaaacg
       61 tatttaaaag aaacatgtaa agaatgcttg ttttatcctt atttgctagt tcaaggtgaa
      121 gctggcatag gaaaatcaca ccttttagcg catctatcta aaaaacttag agatgaaaat
      181 catattattt atctgttttt gggacaattt tttactaaaa atgaagatcc atggcatcaa
      241 atacttaatg atttagaagt tacaaattct gttgataatt ttttaaggtc aataagtaat
      301 aaggccaaag aaacaaaaaa agagctttta ttattattga tgcgcttaat gaaggtgaag
      361 gtaaaaggtt atggggaaat tattttcaaa gctttatcaa ccatataaag aagtattcta
      421 atatagcttt aatattttct attagaaccc cttttgaaga tgttatatta cccaaaaacg
      481 caatacaaga taacaatatt gtagtatttc agcatgaagg ttttagtaag gaagaaaact
      541 ataatccaat tgtatctttt tgtgattttt atggattaga gctacctaag ttacctatat
      601 taaatccaga attcaacaa
//
LOCUS       DAMZKC010000376          594 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.381, whole genome shotgun sequence.
ACCESSION   DAMZKC010000376 DAMZKC010000000
VERSION     DAMZKC010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.381"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            271..594
                     /locus_tag="POB55_002843"
                     /pseudo
     CDS             271..594
                     /locus_tag="POB55_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          232 a           84 c           87 g          191 t
ORIGIN      
        1 tgtaatttta ctttgaaata ctttaaaaaa ataagacact ttgcccaact tgcacataaa
       61 tgtaaaattc aataaaataa attttctgtg ttggatccct tcgtataatt taataaatac
      121 tactaaacta aattaacgag gtgccttatg tataaaaatt ataacatgac ccaacttaca
      181 ctaccaatag aaacttctgt tagaattcct caaaatgata atacgcgata tgttaatgaa
      241 attgttgaaa cgatatctga tagcgaattc gatgaattta tatgtcctaa taacaaaaga
      301 ataggtttta aaagatatgc ataccgtaat gatagatata gttttaaacg tgacttcaag
      361 ctatatgaat gtgatgactg ttcatcatgt tctttgagac atcaatgcat gaagccaaat
      421 tcgaaatcca ataagaaaat tatgaagaat tataattggg aatactttaa agcccaaatt
      481 aatcaaaagc tttctgaacc agaaacgaaa aaatctatag tcaaagaaaa attgatgtag
      541 agcctgtttt tggatttatg aaggctattt tgggtttcac tcgaatgtca gttc
//
LOCUS       DAMZKC010000377          573 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.382, whole genome shotgun sequence.
ACCESSION   DAMZKC010000377 DAMZKC010000000
VERSION     DAMZKC010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.382"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..417
                     /locus_tag="POB55_002844"
     CDS             <1..417
                     /locus_tag="POB55_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDI6501734.1"
                     /translation="PITVNPLTGEKVGEGQPTEEITKQPVDKIVEFGGEKPKDPKGPE
                     NPEKPSRPTHPSGPVNPDNPGLSKDRAKPNEPVHSMDKNDKVKKSKIAKKSVANQEKK
                     RAELPKTGAESTQKGLIFSSIIGIAGLMLLARRRKN"
BASE COUNT          241 a           87 c          106 g          139 t
ORIGIN      
        1 ccaatcacag tgaacccatt aacgggtgaa aaagttggcg agggtcaacc aacagaagag
       61 attacaaaac aaccagtaga taagattgta gagtttggtg gcgagaaacc aaaagatcca
      121 aaaggacctg aaaacccaga gaagccgagc agaccaactc atccaagtgg cccagtaaat
      181 cctgacaatc caggattatc gaaagacaga gcaaaaccaa atgaaccagt tcattcaatg
      241 gataaaaatg ataaagttaa aaaatctaaa attgctaaaa aatcagtagc taatcaagag
      301 aaaaaacgag cagaattacc aaaaacaggt gcagaaagca cacaaaaagg attgatcttt
      361 agtagtataa ttggaattgc tggattaatg ttattggcac gtagaagaaa aaattaaaat
      421 aattcattat ttaaataata cttgatttgc attcactata tttagtttgt taaaaacaac
      481 ctagaatatg gtgaaaatga tatacacccc aaaagttgga ttgaaagacc taactttaag
      541 gggtgtattt ttgttataaa tttgattttt tga
//
LOCUS       DAMZKC010000378          573 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.383, whole genome shotgun sequence.
ACCESSION   DAMZKC010000378 DAMZKC010000000
VERSION     DAMZKC010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.383"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            77..159
                     /locus_tag="POB55_002845"
     tRNA            77..159
                     /locus_tag="POB55_002845"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:110..112,aa:Leu,seq:gag)
     gene            181..254
                     /locus_tag="POB55_002846"
     tRNA            181..254
                     /locus_tag="POB55_002846"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:213..215,aa:Gly,seq:tcc)
     gene            373..>573
                     /locus_tag="POB55_002847"
     rRNA            373..>573
                     /locus_tag="POB55_002847"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          179 a          107 c          129 g          158 t
ORIGIN      
        1 ttattgacaa atatttttaa acataatata ataaataacg ttctgaaaaa cgaagatgtt
       61 ttaaatgcct atatccgcgg tcgtggcgga atggcagacg cgctaggttg agggcctagt
      121 gggagaaatc ccgtggaggt tcaagtcctc tcggccgcat caaaattctt aattaaataa
      181 gcgggtgtag tttaatggca aaacctcagc cttccaagct gatgttgtgg gttcgattcc
      241 catcacccgc tccatagata attttaatga acattgaaaa ctgaatgaca atatgtcaac
      301 gttaattcca aaaacgtaac tataagttac aaacattatt tagtatttat gagctaatca
      361 aacatcataa tttttatgga gagtttgatc ctggctcagg atgaacgctg gcggcgtgcc
      421 taatacatgc aagtcgagcg aacggacgag aagcttgctt ctctgatgtt agcggcggac
      481 gggtgagtaa cacgtggata acctacctat aagactggga taactttggg aaaccggagc
      541 taataccgga taatattttg aaccgcatgg ttc
//
LOCUS       DAMZKC010000379          568 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.384, whole genome shotgun sequence.
ACCESSION   DAMZKC010000379 DAMZKC010000000
VERSION     DAMZKC010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.384"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..>568
                     /gene="fmhC"
                     /locus_tag="POB55_002848"
     CDS             <1..>568
                     /gene="fmhC"
                     /locus_tag="POB55_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FemA/FemB family glycyltransferase FmhC"
                     /protein_id="HDI6501735.1"
                     /translation="KNDKNEVIAACLLTEARIFKFYKYFYSHRGPLLDYFDAKLVCYF
                     FKELSKFIYKNRGVFILVDPYLIENLRDANGRIIKNYNNSVIVKMLGKIGYLHQGYTT
                     GYSNKSQIRWISVLDLKDKDENQLLKEMEYQTRRNIKKTIEIGVKVEDLSIEETNRFY
                     KLFQMAEEKHGFHFMNEDYFKRMQEIYKD"
BASE COUNT          232 a           50 c           95 g          191 t
ORIGIN      
        1 gaagaatgat aaaaatgaag ttatagctgc atgtttatta acagaggcac gaatttttaa
       61 attctacaaa tatttctact ctcatagagg tcctttactt gattatttcg atgctaaatt
      121 agtttgttac ttttttaaag aattatctaa attcatttat aaaaatagag gagtatttat
      181 tcttgttgat ccatatttaa tagagaattt aagagatgca aatggtagga taataaagaa
      241 ttataataat tcagtgatag taaagatgct agggaaaatt gggtatctcc atcaaggtta
      301 tacaacagga tattcaaata aaagtcaaat taggtggatt tctgtattgg atttaaaaga
      361 taaagatgag aatcaacttt taaaagaaat ggaataccaa actagaagaa atataaaaaa
      421 gactattgag attggtgtta aggttgaaga tttatctatt gaagaaacaa atcgatttta
      481 taaattattt caaatggctg aagaaaaaca tggttttcat ttcatgaatg aagattattt
      541 taaacgaatg caagaaatat ataaagat
//
LOCUS       DAMZKC010000380          568 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.385, whole genome shotgun sequence.
ACCESSION   DAMZKC010000380 DAMZKC010000000
VERSION     DAMZKC010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.385"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..>568
                     /locus_tag="POB55_002849"
     CDS             <1..>568
                     /locus_tag="POB55_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDI6501736.1"
                     /translation="LNQHVCIIRLKKEYYYNFFGQYLLSRKGKRKIFLAQSGGSREGL
                     NFKEIANLKIFTPTIFEEQQKIGEFISKLDRQIELEEQKLELLQQQKKGYMQKIFSQE
                     LRFKDEEGKDYPDWKSKSIQEIFENKGGTALETEFNFDGNYKVISIGSYSINSTYNDQ
                     NIRVNKNKKTEKYILSKGDLAMVLNDKTK"
BASE COUNT          253 a           67 c           92 g          156 t
ORIGIN      
        1 tttaaatcaa catgtatgta ttattagatt gaaaaaagag tattattata atttttttgg
       61 acagtatcta ttatcaagaa aaggtaaaag gaaaattttc cttgcacaaa gtggaggtag
      121 tcgagaagga ctaaacttca aagaaattgc taatttaaaa atcttcaccc caactatatt
      181 tgaagagcag caaaaaatag gcgaattcat cagcaaactt gaccgacaaa ttgaattaga
      241 agaacaaaaa cttgaattac ttcagcaaca gaaaaaaggc tatatgcaga aaatcttctc
      301 gcaagaattg cgattcaaag atgaggaagg taaagattat ccagattgga aatcaaaatc
      361 aattcaagaa atatttgaga ataagggtgg cactgctcta gaaacagaat ttaattttga
      421 cggtaattat aaagttataa gtataggaag ttattctata aatagcactt ataatgatca
      481 aaatataaga gtcaataaaa ataaaaaaac tgaaaaatat attttatcaa aaggcgactt
      541 agcaatggta ttaaatgata aaacaaaa
//
LOCUS       DAMZKC010000381          566 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.386, whole genome shotgun sequence.
ACCESSION   DAMZKC010000381 DAMZKC010000000
VERSION     DAMZKC010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.386"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..537
                     /locus_tag="POB55_002850"
     CDS             <1..537
                     /locus_tag="POB55_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501737.1"
                     /translation="GFNLYRHQMQHLSLSAMLLSITINAFINTFIIDKYNFITLCTIT
                     MFIIWTILQFYIDKKLKPVYITDQKFIAIILTIVVSLTQRVTDFSSTQSIYMSIPFLA
                     PAIFIIGGIMLFISTFNSLDETAENNNKIKKLMIKGLIIINISFIVMMVLTPYWYLYL
                     IVYLIFLLFLLWQKVYKF"
BASE COUNT          196 a           81 c           67 g          222 t
ORIGIN      
        1 ggattcaatt tatataggca tcaaatgcaa catttatcat taagtgcaat gttgttatct
       61 attacgataa acgcctttat aaatacattc ataattgata aatataactt tattactctg
      121 tgtactataa cgatgtttat catttggacg atactacagt tttacattga taaaaaatta
      181 aaacctgtat acattacaga ccaaaaattt attgctatca tattaacgat tgttgtcagt
      241 ttaacgcaac gtgttaccga cttttcaagt acacaatcaa tatatatgtc tattccattt
      301 ttagcccctg caattttcat cattggtggt attatgttgt ttatttcaac atttaatagc
      361 ttagatgaaa ctgctgaaaa taataacaaa ataaagaaac taatgattaa aggacttatc
      421 attattaaca tttcatttat cgttatgatg gttttaacac catattggta cttgtattta
      481 atcgtctatc ttattttctt gttgttttta ttgtggcaaa aggtttataa attttaatac
      541 caaaactata aaacacttct gatatt
//
LOCUS       DAMZKC010000382          555 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.387, whole genome shotgun sequence.
ACCESSION   DAMZKC010000382 DAMZKC010000000
VERSION     DAMZKC010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.387"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(119..514)
                     /gene="spxA"
                     /locus_tag="POB55_002851"
     CDS             complement(119..514)
                     /gene="spxA"
                     /locus_tag="POB55_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HDI6501738.1"
                     /translation="MVTLFTSPSCTSCRKAKAWLQEHDIPYTERNIFSEHLTIDEIKQ
                     ILKMTEDGTDEIISTRSKTYQKLNVDIDSLPLQDLYSIIQDNPGLLRRPIILDNKRLQ
                     VGYNEDEIRRFLPRKVRTFQLQEAQRMVD"
BASE COUNT          187 a           82 c           87 g          199 t
ORIGIN      
        1 atttgattta aaagatacta agcaattatt gtaaaattaa aaccaaaaaa gcaacactat
       61 agaaatagtg ttgcttgaca tatcttttag taaagtatga cacatagtta aatggttatt
      121 agtcaaccat acgttgtgct tcttgtaatt ggaacgtacg aacttttcta ggtaagaaac
      181 gtcgaatctc gtcctcatta taaccaactt gtagtcgttt attatctaaa ataattggac
      241 gacgtaataa gccaggatta tcttgaatga ttgaatataa gtcttgtaat ggtagtgaat
      301 caatatcaac atttaatttt tggtatgttt tagaacgtgt agaaatgatt tcatcagtac
      361 cgtcttcagt catttttaat atttgcttaa tttcatcaat tgttaaatgt tcagaaaaaa
      421 tattacgctc cgtatacgga atgtcatgtt cttgtaacca tgctttcgct ttacggcaag
      481 atgtgcaact tggtgaagta aataatgtta ccatacatct cactctccta tttgaatgaa
      541 taaaattcat tgctt
//
LOCUS       DAMZKC010000383          550 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.388, whole genome shotgun sequence.
ACCESSION   DAMZKC010000383 DAMZKC010000000
VERSION     DAMZKC010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.388"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..100
                     /locus_tag="POB55_002852"
     CDS             <1..100
                     /locus_tag="POB55_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501739.1"
                     /translation="TLITGIIMHITMYFVPFESRKMPLFMTIIFTI"
     gene            362..>550
                     /locus_tag="POB55_002853"
     CDS             362..>550
                     /locus_tag="POB55_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501740.1"
                     /translation="MCFDINNLDIKKLNFRKVKNAIHLSLSGDKYQFLEDGKTINNTY
                     FLAVYDNAINIFEDLFSPS"
BASE COUNT          221 a           61 c           91 g          177 t
ORIGIN      
        1 tacattaatt acaggtatca ttatgcatat tacaatgtac tttgtaccat ttgaatctcg
       61 gaaaatgcca ctatttatga cgataatttt tacaatctaa aagcagtaaa gactcataat
      121 ataaccaaaa acattaataa aagtttgtta aatgataagg ggatgagtat tggtaaattt
      181 actcaaaagg tttaagggaa aaatcctacc tggagagatc caaaaactaa atggactatt
      241 tccaaaaata aagggcaatc acatggtggt tcttattgga agttaataaa taacaaagga
      301 aaaagaatag cttctttaac taaagaagga aaaatcttaa gggaataagg tgttgtaaat
      361 tatgtgcttt gatataaata atttggatat aaaaaagttg aattttagaa aggtaaaaaa
      421 cgcaattcat ttaagtttaa gtggcgataa ataccagttt ttggaggacg gtaaaactat
      481 caataatact tattttttag ctgtatatga taatgcaata aacatttttg aagatttgtt
      541 ttcgccttct
//
LOCUS       DAMZKC010000384          549 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.389, whole genome shotgun sequence.
ACCESSION   DAMZKC010000384 DAMZKC010000000
VERSION     DAMZKC010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.389"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(139..339)
                     /gene="cspA"
                     /locus_tag="POB55_002854"
     CDS             complement(139..339)
                     /gene="cspA"
                     /locus_tag="POB55_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspA"
                     /protein_id="HDI6501741.1"
                     /translation="MKQGTVKWFNAEKGFGFIEVEGENDVFVHFSAINQDGYKSLEEG
                     QAVEFEVVEGDRGPQAANVVKL"
BASE COUNT          191 a           96 c           69 g          193 t
ORIGIN      
        1 aaacaatgtt ggtgatataa aaaaagagtt ctcaatttgg ctgagaaact catcgcgtta
       61 agtatttgta ataagcatat aaaaacacct tacttcttgg taaggtgtta gttaaaatca
      121 atgattcaaa tctaagaatt atagtttaac aacgtttgca gcttgtggac cgcggtcgcc
      181 ttcaactact tcaaactcaa cagcttgacc ttcttctaat gatttgtaac catcttggtt
      241 aattgctgaa aaatgtacga atacgtcatt ttctccttca acttcgataa agccgaatcc
      301 tttttcagcg ttaaaccatt taactgtacc ttgtttcata atctgaaacc tccaagacta
      361 aaattcattc aatatgctta ttcgcatatt acaaaaaata catgcaaatt tcactcaaat
      421 attctttgca atatggaata atcatctgct taacttgtat tatagtgcag ttattaccaa
      481 attgcaatac aataataaaa aatattcatt taatcaatta ttggtagttt ataatatact
      541 tcattattt
//
LOCUS       DAMZKC010000385          549 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.390, whole genome shotgun sequence.
ACCESSION   DAMZKC010000385 DAMZKC010000000
VERSION     DAMZKC010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.390"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(<1..214)
                     /locus_tag="POB55_002855"
     CDS             complement(<1..214)
                     /locus_tag="POB55_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HDI6501742.1"
                     /translation="MFLCKRQIDINARFGLPRIAFMSAVATIIMFLVSYEVMYFLSNT
                     PLSDRHFLIFLLLVFMTYPLHKSIHLL"
BASE COUNT          215 a           75 c           94 g          165 t
ORIGIN      
        1 ataacaaatg tatactttta tgtaatggat acgtcataaa tacaagtaat aaaaagatga
       61 gaaaatgtct atctgataat ggcgtattag ataaaaaata cattacttca taactaacta
      121 aaaacataat gatggttgca actgcactca taaatgcaat tctaggcaaa ccaaatcgtg
      181 cattgatatc aatttgtctt ttacataaaa acatatctta cgcctcatct cttttcaata
      241 taagttagat tataactaac tcaggttcag taattcaata gtaacatatc atgttagaag
      301 aattgttata taagaagaaa ggggcgaagt attgtttgat ttgaaacgcg aaagtggagt
      361 ttggtaaaat tggctgattg actagcgtag aagtagaatg cagaacaaat ttctgcgaac
      421 acatcttctt gctaataaag catcacttat gaaaaaggcg cgtgtgacat gagtatttga
      481 aaacttgaat aatataaaaa atggatatgg cagggcatat gcaaaagcat cttttgacca
      541 actaagtta
//
LOCUS       DAMZKC010000386          548 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.391, whole genome shotgun sequence.
ACCESSION   DAMZKC010000386 DAMZKC010000000
VERSION     DAMZKC010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.391"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..180
                     /locus_tag="POB55_002856"
     CDS             <1..180
                     /locus_tag="POB55_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001836194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HDI6501743.1"
                     /translation="VFTYLLLGGCSILFLIWLMTIKPQNLLVFIMWIIITIFFFLISM
                     GSISNKKVYAKLKKQ"
     gene            313..>548
                     /locus_tag="POB55_002857"
     CDS             313..>548
                     /locus_tag="POB55_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HDI6501744.1"
                     /translation="MLLYESNIINKNPRYRIIKYKNDYLMIDLVSTWLVLFFPFINWL
                     IPKKYVQISREEFDNLNIVKPVKNKALWPVIGSIL"
BASE COUNT          212 a           63 c           79 g          194 t
ORIGIN      
        1 gttttcacat atctattatt aggaggttgt tctatattat ttttaatatg gctaatgact
       61 ataaaacctc aaaatctact ggtatttatt atgtggatta ttattacaat ttttttcttt
      121 ctaataagca tgggctcaat tagtaataaa aaggtttatg ccaaacttaa aaagcaatag
      181 taattactaa acttagattg tagttcgtaa gttaagtaat aaacaggaaa atagtatgtt
      241 actaaaatac aaaacactta taaagtatct gaaagtgtat tagatataaa accaacgaga
      301 cggaagtggt aaattttgct ttacgaatct aatattatta acaaaaatcc cagatataga
      361 attattaaat ataaaaacga ctatctgatg attgatttag taagcacatg gttagttttg
      421 ttctttccgt ttattaattg gttaatccca aaaaaatatg tccaaatcag cagagaagag
      481 tttgataatt taaatattgt taaaccagtt aaaaataaag ctttatggcc agttataggt
      541 agcatact
//
LOCUS       DAMZKC010000387          540 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.392, whole genome shotgun sequence.
ACCESSION   DAMZKC010000387 DAMZKC010000000
VERSION     DAMZKC010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.392"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
BASE COUNT          223 a           85 c           63 g          169 t
ORIGIN      
        1 aagattgttg atgagaattt tgagatgtag ataaatatca aactagtcta taggtaaaaa
       61 tttataatta atttgaaaag aatgtctcaa ataatgtttg aattagcagg tgtcatacta
      121 agtaatgtta agtaaacatt ttatacttta tcagaacaaa aaagacatgc atcaacatgt
      181 cagtgaaact aatccatatt atcttaacaa cgaaattgaa tcgaacctga aaaacgtatt
      241 tacaaatcgc aactactcta tccaaggcaa aatcgcaaaa tctgatcccc gaaataacat
      301 atctcgaaaa aaattccata attcaatcat cttcaatatt taacaataca ttactagttc
      361 caaatatact catacataca tgtttcattg attgactgca tattgtcata aataataggg
      421 gaactaatcg ctaataatgt tgttaaacac aatacatagt aaagtgaaag attataaata
      481 catgaattgt tcaccattcc gccatatatt ttcaatcacc taatttccga ataaatatta
//
LOCUS       DAMZKC010000388          535 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.393, whole genome shotgun sequence.
ACCESSION   DAMZKC010000388 DAMZKC010000000
VERSION     DAMZKC010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..535
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.393"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..525
                     /locus_tag="POB55_002858"
     CDS             <1..525
                     /locus_tag="POB55_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001039417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cna B-type domain-containing protein"
                     /protein_id="HDI6501745.1"
                     /translation="NNNQDGKRPTEIKVELYQDGKATGKTATLNESNNWTHTWAGLDE
                     KAKGQQVKYTVEELTKVKGYTTHVDNNDMGNLIVTNKYTPEKPNKPIYPEKPKDKTPP
                     TKPDHSNKVKPTPPDKPSKVDKDDQPKDNKTKPENPLKELPKTGMKIITSWITWVFIG
                     ILGLYLILRKRFNS"
BASE COUNT          245 a           88 c           84 g          118 t
ORIGIN      
        1 aataataacc aagacggaaa acgaccaact gaaattaaag ttgagttata tcaagatgga
       61 aaagcaacag gaaaaacggc aacattaaat gaatctaata actggaccca tacgtgggca
      121 ggattagatg aaaaagcaaa aggtcaacaa gtaaaataca cagtcgagga attaacaaaa
      181 gttaaaggtt atacaacaca tgtggataac aatgatatgg gtaacttgat tgtgacgaat
      241 aaatatacgc cagaaaaacc gaataaacca atctatcctg aaaaaccaaa agacaaaaca
      301 ccaccaacta aacctgatca ctctaataaa gttaaaccaa ctcccccaga taagccatca
      361 aaagtggata aggatgatca acctaaagat aataaaacca aacctgaaaa tcctctaaaa
      421 gaattaccaa aaactggtat gaagattata acttcatgga ttacatgggt atttataggt
      481 atattgggat tgtatttaat tttaagaaaa agatttaact cataaaccat tataa
//
LOCUS       DAMZKC010000389          509 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.394, whole genome shotgun sequence.
ACCESSION   DAMZKC010000389 DAMZKC010000000
VERSION     DAMZKC010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.394"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            19..>509
                     /locus_tag="POB55_002859"
     CDS             19..>509
                     /locus_tag="POB55_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6501746.1"
                     /translation="MKKLYKNRSFSNLLMSRILINAGDSLYYVSTTWIVLQWTQNPLL
                     VGLTNTLLMIPMCISFLFGPLVDALPLKKTLFLTPLLQCLLLSLIASLNLIGYLNVYG
                     LIILVAIAMFCTQIGYPAQSKAIPLLVKKDQLLDANSAMSVAYQGTDVIMNSISGIIV
                     AAIA"
BASE COUNT          161 a           73 c           78 g          197 t
ORIGIN      
        1 atgaataggt gtgatatttt gaaaaagtta tataaaaatc gttcgttttc taatttactt
       61 atgagtagaa ttttgataaa tgctggtgat agtctctatt atgtttctac aacttggata
      121 gttttacaat ggacgcaaaa ccctttacta gttggtttaa ctaatacttt attaatgatt
      181 cctatgtgta tttcattttt atttggtcct ttagtagatg ccttaccatt aaagaagact
      241 ctatttttaa cccctttatt acaatgtcta ttactatcac taattgcttc tttaaaccta
      301 attggatact taaatgtata cggcttgatc attttagtgg caattgcaat gttttgcact
      361 caaataggat acccagctca gtctaaagct atacctttat tagtaaaaaa agatcaatta
      421 ttagatgcaa attctgctat gagtgtcgca tatcaaggaa cagatgttat tatgaattca
      481 ataagtggta ttattgttgc agcaatagc
//
LOCUS       DAMZKC010000390          509 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.395, whole genome shotgun sequence.
ACCESSION   DAMZKC010000390 DAMZKC010000000
VERSION     DAMZKC010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.395"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..>509
                     /locus_tag="POB55_002860"
     CDS             <1..>509
                     /locus_tag="POB55_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5080 family protein"
                     /protein_id="HDI6501747.1"
                     /translation="EKIVVLPIIIYAIVFVVIGITYIFIGDSYDQLTNFNVILYMGSL
                     FYAWMAFRNLWNRPLLLKYKNITDSSSGIVNKSEYNSVESLRINIEIAKYKGIISLIV
                     AIVLTVLMTLKSTPQITAETRDLSISFFILSLFIIIIFAVWDLIIRVRKGAFAFVVIR
                     PILFSCWLF"
BASE COUNT          167 a           57 c           90 g          195 t
ORIGIN      
        1 ctgaaaaaat agtagtattg cctataatca tctatgccat tgtgtttgta gtaattggta
       61 tcacttatat ttttataggt gacagctatg atcagttaac aaatttcaat gtgattttgt
      121 atatggggag tttgttttat gcatggatgg cttttagaaa tctttggaac agaccattat
      181 tattaaaata taagaacatt acagatagtt caagtggaat agttaataaa tctgaatata
      241 attcagttga aagcttacgt ataaacattg aaatagctaa gtataaaggg attatttctt
      301 tgatagtagc tatagtacta acggtattaa tgacattaaa gtcaacgcct caaattactg
      361 cggaaacacg tgacttaagt atctcatttt tcatactcag cttatttatc attattatat
      421 ttgctgtttg ggatttaatt attagagtta gaaaaggagc gtttgctttt gttgtaataa
      481 ggccaatatt attcagttgt tggttattt
//
LOCUS       DAMZKC010000391          507 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.396, whole genome shotgun sequence.
ACCESSION   DAMZKC010000391 DAMZKC010000000
VERSION     DAMZKC010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.396"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            95..>507
                     /locus_tag="POB55_002861"
     CDS             95..>507
                     /locus_tag="POB55_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HDI6501748.1"
                     /translation="MTENNKLQTIEQQLVQEKNVSDNVLNKVRVLESQGNLELPNDYS
                     PSNAMKQAWLQISQDNKLMSCNDTSKANALLDMVTQGLNPAKNQCYFIPYGNKMQLQR
                     SYHGNVMMLKRDAGAQDVVAQVIYKGDTFKQEMGET"
BASE COUNT          219 a           76 c           89 g          123 t
ORIGIN      
        1 gaatcagtaa cagagcttat caaaacagaa tcacaacaaa ttcaattgat agtaaatgaa
       61 caagataaaa aattaagaat ggagactata taaaatgact gaaaataata aattacaaac
      121 tattgaacaa caattagtac aagaaaagaa cgtatctgac aacgtattaa acaaagtgag
      181 agttttagag tcacaaggca atttggaatt gccaaatgat tattcaccaa gtaatgccat
      241 gaaacaagca tggttacaaa tcagccaaga taacaaatta atgagttgta acgatacaag
      301 caaagcaaat gccttattag acatggtaac gcaaggttta aatccagcta aaaatcaatg
      361 ctactttatt ccttacggca acaaaatgca gttacaacgt agctatcacg gtaatgtaat
      421 gatgttaaaa cgtgatgcag gtgctcaaga tgttgttgct caagtgattt ataaaggcga
      481 tacattcaag caagaaatgg gagaaac
//
LOCUS       DAMZKC010000392          496 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.397, whole genome shotgun sequence.
ACCESSION   DAMZKC010000392 DAMZKC010000000
VERSION     DAMZKC010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.397"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..326
                     /locus_tag="POB55_002862"
     CDS             <1..326
                     /locus_tag="POB55_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDI6501749.1"
                     /translation="DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSESDADSDTDS
                     DSDAGKHTPAKPMSTVKDQHKTAKALPETGSENNNSNNGTLFGGLFAALGSLLLFGRR
                     KKQNK"
BASE COUNT          187 a           82 c           93 g          134 t
ORIGIN      
        1 cagactcaga tagcgattca gactcagact cggatagcga ttcagattca gacagcgact
       61 cagattcgga cagcgattca gactcagata gcgactcaga ctcagaaagt gatgccgatt
      121 cagatacaga tagcgattca gatgcaggta aacatactcc ggctaaacca atgagtacgg
      181 ttaaagatca gcataaaaca gctaaagcat taccagaaac aggtagtgaa aataataatt
      241 caaataatgg cacattattc ggtggattat tcgcggcatt aggatcatta ttgttattcg
      301 gtcgtcgtaa aaaacaaaat aaataatatg attaacttaa ccaggtccat gtggcctggt
      361 tttttctgtt tagaaattcc cagatataaa acaaaaaacc tttaaggaat agatacaatt
      421 taatttgtta cagaaatgta attgtctatg aattagaaaa gctaagatat taattttaag
      481 ggcaaataat actatt
//
LOCUS       DAMZKC010000393          466 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.399, whole genome shotgun sequence.
ACCESSION   DAMZKC010000393 DAMZKC010000000
VERSION     DAMZKC010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..466
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.399"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
BASE COUNT          203 a           58 c           59 g          146 t
ORIGIN      
        1 tatatattta tgccaccgct caaactttta taaaaagtaa aaaaacaata tttatattga
       61 acttgtgacg tgaatttcta aaacacaacc aacaatccac aaggaaacta gtaaataagt
      121 ttaaataaga tttcgaaaaa atgtgtaata ttataatatg aaaatcttta agattataga
      181 agaatcatac tgactaaaaa gattttgaaa gtgattaagt atttaaaaca cgccaatctt
      241 aaaccgctat tgacaaatat gaagcggaac caccgttgtt tttcttttca ggtccaattg
      301 aataaatgtt atgaatagaa tggataaaaa tataattatt taagtgttaa agtaaaccaa
      361 atcatgattt aagattataa gaaagaaaaa ttgactaatg ttaaaaccaa caatcatatg
      421 ggttgtaacg tttatacaat catcgtaaaa ctcaatatgc ttaatt
//
LOCUS       DAMZKC010000394          465 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.400, whole genome shotgun sequence.
ACCESSION   DAMZKC010000394 DAMZKC010000000
VERSION     DAMZKC010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.400"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(<1..407)
                     /gene="sarU"
                     /locus_tag="POB55_002863"
     CDS             complement(<1..407)
                     /gene="sarU"
                     /locus_tag="POB55_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarU"
                     /protein_id="HDI6501750.1"
                     /translation="MDYQTFEKVNKFINVEAYIFFLTQELKQQYKLSLKELLILAYFY
                     YKNEHSISLKEIIGDILYKQSDVVKNIKSLSKKGFINKSRNEADERRIFVSVTPIQRK
                     KIACVINELDKIIKGFNKERDYIKYQWAPKYSK"
BASE COUNT          140 a           70 c           54 g          201 t
ORIGIN      
        1 tctttgctat attttggagc ccattgatat tttatgtagt ctctttcctt attaaatcct
       61 ttaattattt tatctaactc attaataaca caagcaatct ttttacgttg tattggagta
      121 actgaaacaa aaatacggcg ttcatctgct tcgtttctag acttatttat aaatcctttt
      181 ttagatagtg acttaatgtt ctttacaaca tcagattgtt tgtaaagtat gtcaccaatg
      241 atttctttta gtgaaatact gtgttcattt ttgtaataaa aatatgctaa tatcaacaat
      301 tcttttaatg ataatttata ttgttgcttt aactcttgag tgagaaaaaa tatgtacgct
      361 tctacattta tgaatttatt gaccttttcg aaagtttgat agtccacctt acattctcct
      421 actattttct gatgtcccaa tactttatat tatttaaagt aatat
//
LOCUS       DAMZKC010000395          460 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.401, whole genome shotgun sequence.
ACCESSION   DAMZKC010000395 DAMZKC010000000
VERSION     DAMZKC010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.401"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(<1..>460)
                     /locus_tag="POB55_002864"
     rRNA            complement(<1..>460)
                     /locus_tag="POB55_002864"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           95 a          142 c           96 g          127 t
ORIGIN      
        1 ttcaccgcta cacatggaat tccactttcc tcttctgcac tcaagttttc cagtttccaa
       61 tgaccctcca cggttgagcc gtgggctttc acatcagact taaaaaaccg cctacgcgcg
      121 ctttacgccc aataattccg gataacgctt gccacctacg tattaccgcg gctgctggca
      181 cgtagttagc cgtggctttc tgattaggta ccgtcaagat gtgcacagtt acttacacat
      241 atgttcttcc ctaataacag agttttacga tccgaagacc ttcatcactc acgcggcgtt
      301 gctccgtcag gctttcgccc attgcggaag attccctact gctgcctccc gtaggagtct
      361 ggaccgtgtc tcagttccag tgtggccgat caccctctca ggtcggctat gcatcgttgc
      421 cttggtaagc cgttacctta ccaactagct aatgcagcgc
//
LOCUS       DAMZKC010000396          453 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.402, whole genome shotgun sequence.
ACCESSION   DAMZKC010000396 DAMZKC010000000
VERSION     DAMZKC010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.402"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..215
                     /locus_tag="POB55_002865"
     CDS             <1..215
                     /locus_tag="POB55_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501751.1"
                     /translation="KVIDDIFKNYEQIDPTIEIENGNTKLKLNGYFIASIISPLNLNK
                     LNNLYVEEEFYHTYNELIVKYTEVKE"
     gene            221..>453
                     /locus_tag="POB55_002866"
     CDS             221..>453
                     /locus_tag="POB55_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 1"
                     /protein_id="HDI6501752.1"
                     /translation="MKKIFMMVHELDVNKGGMTSSMFNRSKEFYDADIPADIVTFDYK
                     GNYDEIIKALKKQGKMDRRTKMYNVFEYFKQIS"
BASE COUNT          197 a           40 c           70 g          146 t
ORIGIN      
        1 taaaagtcat tgacgatata tttaaaaatt atgagcagat agatccaact atagaaatcg
       61 aaaatggaaa tacaaaatta aaattaaatg gatattttat tgcgtcaatt ataagcccat
      121 taaatttaaa taaacttaat aatttatatg ttgaagaaga attttatcat acatataacg
      181 aattaattgt taaatatact gaggtaaagg aataattata atgaaaaaaa tatttatgat
      241 ggtacatgag ttagatgtca ataaaggtgg tatgacctct tcgatgttca atagaagtaa
      301 agagttttat gatgcggaca tacctgctga tattgttact ttcgattaca aaggaaacta
      361 tgatgaaatt attaaagctt tgaaaaaaca aggtaaaatg gatcgaagaa cgaaaatgta
      421 taatgtattt gaatatttta aacaaatttc aaa
//
LOCUS       DAMZKC010000397          452 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.403, whole genome shotgun sequence.
ACCESSION   DAMZKC010000397 DAMZKC010000000
VERSION     DAMZKC010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.403"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            12..209
                     /locus_tag="POB55_002867"
     CDS             12..209
                     /locus_tag="POB55_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501753.1"
                     /translation="MTELNNIINSLQSLFESESGYKISKNSGVPYQTVQDLRNGKTKL
                     EDARFRTIIKLYSYYVSLKEH"
BASE COUNT          179 a           67 c           82 g          124 t
ORIGIN      
        1 ggggattaaa aatgacagag ttgaataata ttataaactc tcttcaatct ttgtttgagt
       61 ctgaatcagg ctataaaatt tcgaaaaatt caggagttcc atatcaaaca gtacaagatt
      121 taagaaatgg gaaaaccaaa ctagaagatg ctagatttag aacgattatt aaactttata
      181 gttactatgt ctcattaaaa gaacactaat catgggacaa taaagtattg ctataataat
      241 tgaatcatta aatgaaggaa tagagcctaa gacataataa atcaatgtct taggctctac
      301 aatattatat tggtagtagt tgaccgaatg aaaatgccca tgtaacaagc atttttcaat
      361 tataggcggg gccccaacac agaagctgac gaaaagtcag cttacaataa tgtgcaagtt
      421 ggcggggccc caacatagag aatttcaaaa ag
//
LOCUS       DAMZKC010000398          451 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.404, whole genome shotgun sequence.
ACCESSION   DAMZKC010000398 DAMZKC010000000
VERSION     DAMZKC010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.404"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
BASE COUNT          177 a           41 c           73 g          160 t
ORIGIN      
        1 aaacatctca ttcaataatt aatatgatcg aaagtttcat gaaaatatat tagtcgattt
       61 tgaaagaagc gtagtcaatt tatgtctttc tataaaaatt tactattaag aaaatctctg
      121 cgtatttttc tataatcagt ttcgtattta tacgattctg gagttcaata ttaatgtctt
      181 atatttaaaa tgccatacct atgcaataga acatatattc tttttgtgag tagaaagttt
      241 gttatattag aagtgaataa ttttaaaaca tattatggaa atgtaagaag gagtattatg
      301 aaactaggta aagtagattg ttgaaaattc catacaatag gagaagtaaa gtgaataaaa
      361 tctggtatta ctaaagctat tgattgtcct aattgtaata aaaagttgga tagtgtagtt
      421 gaggggagaa aaactaaaat gcaataatat a
//
LOCUS       DAMZKC010000399          448 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.405, whole genome shotgun sequence.
ACCESSION   DAMZKC010000399 DAMZKC010000000
VERSION     DAMZKC010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.405"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            18..362
                     /locus_tag="POB55_002868"
     CDS             18..362
                     /locus_tag="POB55_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501754.1"
                     /translation="MTLFDMPNYLWITTLIMILLTIFCCLVLNKWFVSAVITFVILGV
                     LAFFIPNFQDIKYQPLLGYAAFLAIMSLLISFLLWYFTRNWRKERKARKLEKQIEKYG
                     YEGAELRRKDKK"
     gene            310..>448
                     /locus_tag="POB55_002869"
     CDS             310..>448
                     /locus_tag="POB55_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501755.1"
                     /translation="MKNTAMRALNFAVKIKNNIRQLYAQNHRLLSYTAVTIISKEMEY
                     SA"
BASE COUNT          166 a           54 c           72 g          156 t
ORIGIN      
        1 ataaaaggag aatgaatatg acattattcg atatgcctaa ttatttatgg atcacaacat
       61 taataatgat tttattaaca atattctgtt gcttagtttt aaataaatgg tttgtatctg
      121 cagtaattac atttgttata ttaggtgtgc ttgcattttt tattccaaat tttcaagata
      181 taaaatatca accattatta ggatacgctg catttttagc gattatgagt ttattaatta
      241 gttttctatt atggtatttt actagaaatt ggcgtaaaga aagaaaagcg cgtaaattag
      301 aaaagcaaat tgaaaaatac ggctatgagg gcgctgaact tcgccgtaaa gataaaaaat
      361 aatattcgac aactatatgc acaaaatcat cgacttttaa gttacactgc agtaacaatt
      421 atttcgaaag agatggaata cagtgcta
//
LOCUS       DAMZKC010000400          444 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.406, whole genome shotgun sequence.
ACCESSION   DAMZKC010000400 DAMZKC010000000
VERSION     DAMZKC010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.406"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..>444
                     /locus_tag="POB55_002870"
     CDS             <1..>444
                     /locus_tag="POB55_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501756.1"
                     /translation="TVFAEFEIDGKITTFKKESHPKYTINQKTNRKEYSRSRTKKQYI
                     NDESIKVKDYKARIDELIDEDVFKLITNPQAFNLLDWKKRRSLLFEIAKPINDEDVIK
                     TNDDFKELNNILGDHEIETKKKILTDKIKQINKDIKDIPIRINQT"
BASE COUNT          198 a           60 c           72 g          114 t
ORIGIN      
        1 gactgttttc gcagaatttg aaatcgacgg aaaaattacg acttttaaaa aagagtcaca
       61 tcctaaatac acaataaatc aaaaaacgaa tcgcaaggaa tactcacgaa gtcgaacgaa
      121 gaaacaatat atcaatgatg aatcaataaa agtaaaggat tataaagctc gtattgatga
      181 actgattgat gaagatgtat tcaagttaat tacgaaccct caagcattta acttactaga
      241 ttggaagaag cgaagaagtt tgttgtttga aattgctaaa ccaatcaatg atgaggatgt
      301 cattaaaaca aatgatgatt ttaaagaatt aaataatatt cttggagatc atgaaattga
      361 aacaaagaaa aagattctta cggacaagat aaaacagatt aacaaagata tcaaagatat
      421 tccgatacgt attaaccaaa caca
//
LOCUS       DAMZKC010000401          434 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.407, whole genome shotgun sequence.
ACCESSION   DAMZKC010000401 DAMZKC010000000
VERSION     DAMZKC010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.407"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..>434
                     /locus_tag="POB55_002871"
     CDS             <1..>434
                     /locus_tag="POB55_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDI6501757.1"
                     /translation="TDEYKAVMLEINEINQKRSNIRKTIQDKVSGIDDKISELTQEKS
                     EIEVSRSIEKSNKHLDDVISELRNEEDRLLDEKEKYSHDLYILKEFTTTKVKMLTENI
                     NNEFDIAEFKLFNTLVNGELEETCSTTVNGVEYDSGLNNASR"
BASE COUNT          193 a           61 c           70 g          110 t
ORIGIN      
        1 actgacgaat acaaagcagt aatgttagag ataaatgaga ttaatcaaaa acgctctaac
       61 atcaggaaaa ctattcaaga taaagtttca ggaatagatg acaaaataag cgaacttact
      121 caagaaaaat cagaaattga agtgtcaaga tcaatcgaaa aatcaaataa acatctagat
      181 gatgttattt ctgaattaag aaatgaagaa gacagattat tggatgaaaa agaaaagtat
      241 tcacatgacc tttatatctt aaaagaattt acaacaacaa aagtcaaaat gcttactgaa
      301 aatatcaata acgaatttga tattgctgaa tttaagttat tcaatacctt agttaacggc
      361 gaattagaag aaacatgttc cacaacggtt aacggcgtcg aatacgacag cggtttaaat
      421 aacgcctcaa gaat
//
LOCUS       DAMZKC010000402          427 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.408, whole genome shotgun sequence.
ACCESSION   DAMZKC010000402 DAMZKC010000000
VERSION     DAMZKC010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.408"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            84..>427
                     /locus_tag="POB55_002872"
     CDS             84..>427
                     /locus_tag="POB55_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HDI6501758.1"
                     /translation="MTTEMNDVTNHQPNRKSNDENIMAMLIYLLSLFTSIIGPLIIWL
                     LKKDESKLVDRAGKNYLNYTISYIIWSIVLVVITLIGVFLIATDISFIIIIGFIITFI
                     GILSIFAFSILS"
BASE COUNT          137 a           59 c           57 g          174 t
ORIGIN      
        1 aattgtttaa taaacatgtt tatttttgat tatattttgt tatagttaaa gataatatta
       61 caattttcaa aggagtgtct aatatgacaa ctgaaatgaa cgacgtaaca aatcatcaac
      121 cgaatcgcaa atccaatgat gagaacatta tggcgatgct tatctattta ttaagtttat
      181 tcacatctat tattggtcca ctgattatat ggttattgaa aaaagatgaa tcgaaactag
      241 ttgatcgtgc tggaaaaaat tatcttaact atacgatttc ttatattatt tggtctatcg
      301 ttttagtcgt tatcacttta ataggcgttt tcctaattgc taccgatatt agtttcatta
      361 tcattatagg tttcatcatt acatttatcg gtattttatc catattcgct ttctcaatat
      421 tatcttt
//
LOCUS       DAMZKC010000403          417 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.409, whole genome shotgun sequence.
ACCESSION   DAMZKC010000403 DAMZKC010000000
VERSION     DAMZKC010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.409"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
BASE COUNT          184 a           58 c           52 g          123 t
ORIGIN      
        1 agatcttctt attaaatgaa aattaataaa ttatcaaatt gacatacctt attgtattta
       61 tagaaaaaca aaaaaaggta agcaccgaaa tgcttaccta cttcccataa acaatataac
      121 acatatacat tgatttggaa agcgcaaaaa taaatttaat aaactagccc gaaggggagc
      181 aatacataaa aaatgaaagg cgctccttga aaacgcccaa ggtaatatta acataaaatg
      241 gctactattg cattatctaa ttttatttaa ttaaaacaaa tatatataga acaaaaaaac
      301 tagcccgaag gctagcttat gaatagatga aaatttgaac acattgctgt gtctaagata
      361 aatatagcac atattaatta ctattaatag aaaaattata gcgtttttta gattatt
//
LOCUS       DAMZKC010000404          377 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.410, whole genome shotgun sequence.
ACCESSION   DAMZKC010000404 DAMZKC010000000
VERSION     DAMZKC010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.410"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..206
                     /locus_tag="POB55_002873"
     CDS             <1..206
                     /locus_tag="POB55_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Csa1 family protein"
                     /protein_id="HDI6501759.1"
                     /translation="NDDYNVKQLRKRYDIPTNKAPKLLLKGTGNLKGSSVGYKKIEFT
                     FLENKNENIYFTDSLHLKPSEDK"
BASE COUNT          170 a           43 c           56 g          108 t
ORIGIN      
        1 ctaatgatga ttataatgta aaacaattac gtaaaagata tgacatacca acaaataaag
       61 cgccgaagct gttgttgaaa ggtacaggga atttaaaagg ttcatcagtt ggatataaaa
      121 aaattgaatt tactttttta gagaataaaa atgaaaatat ttactttact gatagtctac
      181 atcttaaacc gagcgaggat aaataaacgt gaccaataaa gagtatgaaa tcgaacccgg
      241 aaaaataaca agtaattttg tagaaactag cgcaacatca aatatatgag aatagtcaaa
      301 ttataagtaa ttaacaacgt atgttatttc aaaataggta ttaacctaaa taaataacaa
      361 tattttaaat ttaatag
//
LOCUS       DAMZKC010000405          370 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.411, whole genome shotgun sequence.
ACCESSION   DAMZKC010000405 DAMZKC010000000
VERSION     DAMZKC010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.411"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(75..308)
                     /locus_tag="POB55_002874"
     CDS             complement(75..308)
                     /locus_tag="POB55_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501760.1"
                     /translation="MVFNDWEINVVYSGEHEVVTNEKSLFVIDEFYDVAIAIYYLDGK
                     LKVAHVNYGSDFTIDVANKVFELNIQNPNVDEH"
BASE COUNT          125 a           66 c           37 g          142 t
ORIGIN      
        1 ctaattttca tttgctttac atgtaaaagg ctatatatag tatgctcttt atgattctaa
       61 atgcttttta atatttaatg ctcatcaaca tttggatttt gaatattcaa ttcaaaaact
      121 ttattagcta cgtcaattgt aaaatcagaa ccatagttga catgagctac ttttaatttt
      181 ccatctaaat aatagattgc gattgcaaca tcgtaaaatt cgtcaatgac aaataaactc
      241 ttttcatttg ttacaacctc atgctctcct gagtatacaa cgttaatttc ccaatcatta
      301 aaaaccattt gttaacctcc ttgaacattt aaattgattc aacttaagtt taacttattc
      361 atacaacgtc
//
LOCUS       DAMZKC010000406          363 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.412, whole genome shotgun sequence.
ACCESSION   DAMZKC010000406 DAMZKC010000000
VERSION     DAMZKC010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.412"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..>363
                     /locus_tag="POB55_002875"
     CDS             <1..>363
                     /locus_tag="POB55_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501761.1"
                     /translation="FQAITGMSLFYCLVIFPMTIVVYLRVSQKNYLRSNKIEMIMGTI
                     IAIISLLLIILQAFNITWGVIPITNFGHQFFFFIGIILVIAGIFYKRLEFSGIGLLFC
                     QKTVDAMIHNPQSAQIFS"
BASE COUNT          109 a           52 c           57 g          145 t
ORIGIN      
        1 gtttcaagct attactggaa tgagtctatt ttattgttta gtgatttttc caatgactat
       61 tgttgtatac ctaagggtgt cacaaaagaa ttacttgcgt agtaataaaa tagaaatgat
      121 tatgggtaca attatcgcta tcatatcctt gttattaatt atattacaag catttaatat
      181 tacttggggc gttataccaa ttacaaattt tggacatcaa tttttctttt tcattggtat
      241 tattttagta attgccggca tattttataa gcgacttgag ttttcgggaa tcggcttatt
      301 attttgtcaa aaaaccgtcg atgcaatgat tcataatcca caatcagccc agattttttc
      361 att
//
LOCUS       DAMZKC010000407          362 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.413, whole genome shotgun sequence.
ACCESSION   DAMZKC010000407 DAMZKC010000000
VERSION     DAMZKC010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.413"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..>362
                     /locus_tag="POB55_002876"
     CDS             <1..>362
                     /locus_tag="POB55_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501762.1"
                     /translation="IEDTFHFTLFTAITDLIDKVVNVSSRIIEEASQYDILIALHSLN
                     SLKNELNTIIDKHEENLIRFESLINGYVRNTQLKILVRNTIEQHPELTMKIEDSLISN
                     LNDYNISEIYSRETFNND"
BASE COUNT          149 a           43 c           49 g          121 t
ORIGIN      
        1 attgaggata catttcattt tacattattc actgctataa ctgatttaat tgataaagta
       61 gtcaatgttt caagtcgtat tatcgaagaa gcatctcaat acgatatatt aattgcctta
      121 cacagtttga atagtttaaa aaatgagttg aatacaataa tagataaaca cgaagaaaat
      181 ttaattcgtt tcgaaagtct tattaatggt tatgtaagaa atactcaatt aaaaatatta
      241 gttcgcaata caattgaaca gcacccagaa ctaacaatga aaatagaaga ttcattaatt
      301 tcaaacctta atgattataa tattagtgaa atatatagta gggagacttt taataacgat
      361 ta
//
LOCUS       DAMZKC010000408          362 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.414, whole genome shotgun sequence.
ACCESSION   DAMZKC010000408 DAMZKC010000000
VERSION     DAMZKC010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.414"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..>362
                     /locus_tag="POB55_002877"
     CDS             <1..>362
                     /locus_tag="POB55_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDI6501763.1"
                     /translation="HEENRRNRLLENHKGLKSDLEKAKNQKFEYLDDNVCSCCGQQLP
                     AEQVNEAREKALQKFNANKSKELETIQTSINHIISEGKKIKPIIEKLEDDNNNLQIKI
                     NEAEERSARIQNKINKLKT"
BASE COUNT          174 a           45 c           61 g           82 t
ORIGIN      
        1 catgaagaaa atagacgtaa tcgattatta gaaaatcata aaggattaaa aagtgattta
       61 gaaaaagcta aaaatcaaaa atttgaatat cttgatgata atgtatgtag ttgttgtggt
      121 caacagttac cagctgaaca agtgaatgag gcaagagaaa aagcattgca gaaattcaat
      181 gcaaacaaat cgaaagaatt agaaacaata caaacatcta tcaatcacat tatttcagaa
      241 ggcaagaaaa taaagccaat catcgagaag ttagaggatg acaataataa tcttcaaatt
      301 aaaatcaacg aagcagaaga gcgttcagca agaatacaaa acaaaattaa taagttgaaa
      361 ac
//
LOCUS       DAMZKC010000409          358 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.415, whole genome shotgun sequence.
ACCESSION   DAMZKC010000409 DAMZKC010000000
VERSION     DAMZKC010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.415"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            2..>358
                     /locus_tag="POB55_002878"
     CDS             2..>358
                     /locus_tag="POB55_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001797296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HDI6501764.1"
                     /translation="MEYIKKIALYMSVLLLIIFIGGCGNMKDEQKKEEQTNKTDSKEE
                     QIKKSFAKTLDMYPIKNLEDLYDKEGYRDGEFKKGDKGMWTIYTDFAKSNKADELSNE
                     GMVLYLDRNTRTAKGHY"
BASE COUNT          155 a           33 c           78 g           92 t
ORIGIN      
        1 gatggagtat ataaaaaaaa ttgctttgta catgagtgta ttacttttaa tcatttttat
       61 tgggggatgt ggaaatatga aagatgaaca gaaaaaagag gaacaaacga ataaaacaga
      121 ttcaaaagaa gaacaaatca aaaagagttt tgcgaaaacg ttagatatgt atccaattaa
      181 gaatctcgag gatctatatg acaaagaagg atatcgagat ggtgagttta aaaaaggtga
      241 taaaggtatg tggacgatat atacagattt tgccaaaagt aataaagcag acgaattaag
      301 taatgaaggt atggtcttat acttagatag aaatacacgg acagcaaagg gacattat
//
LOCUS       DAMZKC010000410          335 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.418, whole genome shotgun sequence.
ACCESSION   DAMZKC010000410 DAMZKC010000000
VERSION     DAMZKC010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.418"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..>335
                     /locus_tag="POB55_002879"
     CDS             <1..>335
                     /locus_tag="POB55_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4865 family protein"
                     /protein_id="HDI6501765.1"
                     /translation="YSPLYLWRSSEGMTEFIFNGFYDNVISSFGWQNINIGVIYSMNI
                     TDSVKHSLYALEEYIDIFPTLSLKEIEIKKLFRIFDNAVAEIIIYNPDKWKFVKYSFI
                     ENLPKSLNP"
BASE COUNT          119 a           41 c           51 g          124 t
ORIGIN      
        1 ttattcacca ttatatttat ggagaagttc agaggggatg actgaattta tattcaatgg
       61 attttatgat aatgttattt cttcttttgg atggcaaaat ataaatattg gtgtgatata
      121 ttcaatgaac ataaccgatt ctgttaaaca tagtctttat gcgcttgaag aatatatcga
      181 cattttccct actttatctc taaaagaaat agaaatcaaa aaattgttta gaatcttcga
      241 taatgcagtt gcagaaatta taatttataa tcctgataag tggaagtttg tgaaatattc
      301 atttattgaa aatcttccaa agagtctcaa tccaa
//
LOCUS       DAMZKC010000411          334 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.419, whole genome shotgun sequence.
ACCESSION   DAMZKC010000411 DAMZKC010000000
VERSION     DAMZKC010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.419"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(<1..>334)
                     /locus_tag="POB55_002880"
     CDS             complement(<1..>334)
                     /locus_tag="POB55_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HDI6501766.1"
                     /translation="YLLNIDLGKMIVAQDLNLQEIFFHNLIYFFSAIFGFLTLGLLNI
                     GMLLINSGMLGFFIAHGIYSNQLKSILLVLAPHAIFEILALLIASTFSLGFLSYLYQR
                     MVKKNKIK"
BASE COUNT          136 a           43 c           42 g          113 t
ORIGIN      
        1 ttttttattt tatttttctt taccattctt tgataaaggt atgataaaaa acctaatgag
       61 aatgttgaag caattaaaag tgctaaaatc tcaaatatag cgtgtggcgc taatactaat
      121 agaatagatt ttaattgatt gctatagatt ccatgtgcta taaaaaagcc taacatccca
      181 gaatttatga gtaacatacc tatatttaac agaccaagtg tcagaaaacc aaatattgca
      241 gagaagaaat aaattaaatt atgaaagaaa atctcttgta aatttaaatc ttgagcaact
      301 atcattttac ctaaatcaat atttaataag taat
//
LOCUS       DAMZKC010000412          320 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.422, whole genome shotgun sequence.
ACCESSION   DAMZKC010000412 DAMZKC010000000
VERSION     DAMZKC010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.422"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
BASE COUNT          103 a           59 c           49 g          109 t
ORIGIN      
        1 caccccaact cgcattgcct gtagaatttc ttttcgaaat tctctgtgtt tgggcccctg
       61 actagagttg aaaaaagctt gttgcaagcg cattttcatt cagtcaacta ctgccaatat
      121 aatattatag accctaggac attgatttat ctcccaagct ccatcagtac gatttattat
      181 agaacccgtt ttaagtaatt agagagacga gaatcatttt gtaaatttcg tcagttcaaa
      241 actttaagtg cattattgtt tagcacgacg atctttaata tataatatta attcaattat
      301 aaatacaagg ataagtgata
//
LOCUS       DAMZKC010000413          318 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.423, whole genome shotgun sequence.
ACCESSION   DAMZKC010000413 DAMZKC010000000
VERSION     DAMZKC010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.423"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(<1..>318)
                     /locus_tag="POB55_002881"
     CDS             complement(<1..>318)
                     /locus_tag="POB55_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501767.1"
                     /translation="IKGLVELMNDSEFNQLNYEESYTVVNNIAKEYVQKSNRFLEALI
                     DENILIKNTGYKGEMIIYFSYERMGDYFLSEYLLEKYRNVDKRDLVTKLQSDEKVTRY
                     FQK"
BASE COUNT          103 a           55 c           26 g          134 t
ORIGIN      
        1 tccttttgaa aataccttgt cactttttca tctgactgta atttagttac taaatctctt
       61 ttatcaacat ttctatattt ctctaaaagg tattctgata aaaagtaatc tcccatacgc
      121 tcatacgaaa aataaataat catttcacct ttatagccag tatttttaat taatatattt
      181 tcatctatta gcgcttctaa aaatctattt gacttttgaa catactcttt agctatatta
      241 ttaacaacag tataggattc ctcataattt agttgattaa attctgagtc gttcattaat
      301 tcaactaatc cttttatt
//
LOCUS       DAMZKC010000414          299 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.424, whole genome shotgun sequence.
ACCESSION   DAMZKC010000414 DAMZKC010000000
VERSION     DAMZKC010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.424"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
BASE COUNT          103 a           53 c           37 g          106 t
ORIGIN      
        1 atggagagga ttacttcgta tactgttata actgaaatct tctatatatc atttcgtaat
       61 atcgctagct tcatctaaaa aatttaatgc tatgatttag ccgaattaaa aacatatata
      121 cttatttact atgaactaaa agtttatcct aaaaataaaa aaccatgatt tcgaagtctt
      181 cataaactga agcttctact ctcatggcaa tttatactat gatattcaat tataatgcct
      241 agtctcatag taaacggcta ctacattcgc atgtacttcg ttttactgtc cacgcatgg
//
LOCUS       DAMZKC010000415          286 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.426, whole genome shotgun sequence.
ACCESSION   DAMZKC010000415 DAMZKC010000000
VERSION     DAMZKC010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.426"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            31..228
                     /locus_tag="POB55_002882"
     CDS             31..228
                     /locus_tag="POB55_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI6501768.1"
                     /translation="MKNNLKIARVSLSMTQKELANKVGVTRQTISLIEKGVHNPSLSL
                     CKNICSVLNKNLDEIFGEKPQ"
BASE COUNT          123 a           37 c           47 g           79 t
ORIGIN      
        1 tataaaaaaa agaagccgga ataattaaaa gtgaaaaata atttgaaaat agctagagtt
       61 tcactatcta tgactcaaaa ggagctagca aataaagttg gagtaactag acaaacaata
      121 tctcttatag aaaagggtgt acataacccc tctctatcac tatgtaaaaa tatttgttcg
      181 gtgttgaata aaaatttgga tgagatattt ggagagaaac cacaataaaa gagcttattg
      241 aaagataatc taaaagtcga caatatattt cttctcagaa actgct
//
LOCUS       DAMZKC010000416          279 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.427, whole genome shotgun sequence.
ACCESSION   DAMZKC010000416 DAMZKC010000000
VERSION     DAMZKC010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..279
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.427"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..140
                     /locus_tag="POB55_002883"
     CDS             <1..140
                     /locus_tag="POB55_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDI6501769.1"
                     /translation="HHNKAKALPETGNENSGSNNATLFGGLFAALGSLLLFGRRKKQN
                     K"
BASE COUNT          109 a           41 c           40 g           89 t
ORIGIN      
        1 accatcacaa taaagcaaaa gcattaccag aaacaggtaa tgaaaatagc ggctcaaata
       61 acgcaacgtt atttggcgga ttattcgcag cattaggatc attattgtta ttcggtcgtc
      121 gtaaaaaaca aaataaataa tacagcttat accaggtccg tgaggcctgg ttttttattt
      181 caaaatttat gaaatgtatc ttatgtttga gattaaatag tgcaattttt taaaaaatat
      241 aacataaatc aacataaatt taattcatct ttacattaa
//
LOCUS       DAMZKC010000417          269 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.428, whole genome shotgun sequence.
ACCESSION   DAMZKC010000417 DAMZKC010000000
VERSION     DAMZKC010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..269
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.428"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(1..269)
                     /locus_tag="POB55_002884"
                     /pseudo
     CDS             complement(1..269)
                     /locus_tag="POB55_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499521.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
BASE COUNT           97 a           38 c           30 g          104 t
ORIGIN      
        1 cgctttgaaa ttaatgttct tttcgaaaat ataataaata aaataaacga ttaaaagtgc
       61 ggataacgga acttctatat tcatttgttt aatgacacca acggcaatca tcatatgatc
      121 tattttgtat gattcagcag taacaccata ttcttttaaa aaatttatga atttagacac
      181 gtcaaaattt ttgtctttta caaaataact tcctttgata tcttttttaa taattcgtcg
      241 tcacttaaat aatgaatgtt atatttagc
//
LOCUS       DAMZKC010000418          268 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.429, whole genome shotgun sequence.
ACCESSION   DAMZKC010000418 DAMZKC010000000
VERSION     DAMZKC010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..268
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.429"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..177
                     /locus_tag="POB55_002885"
     CDS             <1..177
                     /locus_tag="POB55_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501770.1"
                     /translation="FNTIKKLIENKEISSYQINKDTGISYGNINAMRRGERRIENLSL
                     KNAKILYEYAKKVL"
BASE COUNT          114 a           27 c           51 g           76 t
ORIGIN      
        1 ttcaatacaa tcaaaaaatt gatagaaaac aaagagatat caagttatca aattaataaa
       61 gatactggga taagttacgg taatattaat gctatgcgcc gtggggaaag aagaatagaa
      121 aatttaagct taaagaatgc aaagatctta tatgaatatg cgaaaaaggt attgtaatat
      181 aagcattgag ttcaataggc atacaataac tgcccaaact agtgactgag tgggtgtttt
      241 tatagtatta aaagttttct aaaagtga
//
LOCUS       DAMZKC010000419          264 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.430, whole genome shotgun sequence.
ACCESSION   DAMZKC010000419 DAMZKC010000000
VERSION     DAMZKC010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..264
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.430"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            <1..>264
                     /locus_tag="POB55_002886"
     rRNA            <1..>264
                     /locus_tag="POB55_002886"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           70 a           62 c           75 g           57 t
ORIGIN      
        1 ctggtctgta actgacgctg atgtgcgaaa gcgtggggat caaacaggat tagataccct
       61 ggtagtccac gccgtaaacg atgagtgcta agtgttaggg ggtttccgcc ccttagtgct
      121 gcagctaacg cattaagcac tccgcctggg gagtacgacc gcaaggttga aactcaaagg
      181 aattgacggg gacccgcaca agcggtggag catgtggttt aattcgaagc aacgcgaaga
      241 accttaccaa atcttgacat cctt
//
LOCUS       DAMZKC010000420          252 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.431, whole genome shotgun sequence.
ACCESSION   DAMZKC010000420 DAMZKC010000000
VERSION     DAMZKC010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.431"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
BASE COUNT           92 a           27 c           45 g           88 t
ORIGIN      
        1 aagacgttta agtcatcagt tacattttgt atttataaat aaagattagc acagcatagt
       61 acaaagttta gaggtaaatg aattaattat tgcatcgcta tttatgaatc gtaaacacta
      121 ttatgaaatt gcaagagtag tatggctaat tagtatgatg gtttgagata aatgcgtgtt
      181 attaaccagt gagtcatctt caaaattaga ggccatttgc tgtttataat aagatgattt
      241 gataatcaac ta
//
LOCUS       DAMZKC010000421          252 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.denovo.432, whole genome shotgun sequence.
ACCESSION   DAMZKC010000421 DAMZKC010000000
VERSION     DAMZKC010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.denovo.432"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            36..209
                     /locus_tag="POB55_002887"
     CDS             36..209
                     /locus_tag="POB55_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501771.1"
                     /translation="MWNFIKCVFKFVFSLVAITTLVAGVGVVAFAYIFKKDFEDIERK
                     TKEIISDIESKNN"
BASE COUNT           96 a           21 c           47 g           88 t
ORIGIN      
        1 agataggtat ttaatttaac agaggtgaaa ttgagatgtg gaattttatt aaatgtgtgt
       61 ttaaattcgt atttagctta gttgctatta caacattagt tgctggtgtt ggtgtagtag
      121 catttgctta tatctttaaa aaagattttg aagatattga aagaaaaact aaagaaatta
      181 tttctgatat tgaaagtaaa aataactaat aacatttaga ggctgggaca taaatcccta
      241 aaaaacagca gt
//
LOCUS       DAMZKC010000422        11623 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.guided.082, whole genome shotgun sequence.
ACCESSION   DAMZKC010000422 DAMZKC010000000
VERSION     DAMZKC010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11623
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.guided.082"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            complement(251..499)
                     /locus_tag="POB55_002888"
                     /pseudo
     CDS             complement(251..499)
                     /locus_tag="POB55_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077893.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(704..2248)
                     /locus_tag="POB55_002889"
     CDS             complement(704..2248)
                     /locus_tag="POB55_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux MFS
                     transporter QacA"
                     /protein_id="HDI6501772.1"
                     /translation="MISFFTKTTDMMTSKKRWTALVVLAVSLFVVTMDMTILIMALPE
                     LVRELEPSGTQQLWIVDIYSLVLAGFIIPLSAFADKWGRKKALLTGFALFGLVSLAIF
                     FAESAEFVIAIRFLLGIAGALIMPTTLSMIRVIFENPKERATALAVWSIASSIGAVFG
                     PIIGGALLEQFSWHSAFLINVPFAIIAVVAGLFLLPESKLSKEKSHSWDIPSTILSIA
                     GMIGLVWSIKEFSKEGLADIIPWVVIVLAITMIVIFVKRNLSSSDPMLDVRLFKKRSF
                     SAGTIAAFMTMFAMASVLLLASQWLQVVEELSPFKAGLYLLPMAIGDMVFAPIAPGLA
                     ARFGPKIVLPSGIGIAAIGMFIMYFFGHPLSYSTMALALILVGAGMASLAVASALIML
                     ETPTSKAGNAAAVEESMYDLGNVFGVAVLGSLSSMLYRVFLDISSFSSKGIVGDLAHV
                     AEESVVGAVEVAKATGIKQLANEAVTSFNDAFVATALVGGIIMIIISIVVYLLIPKSL
                     DITKQK"
     gene            2427..2993
                     /gene="qacR"
                     /locus_tag="POB55_002890"
     CDS             2427..2993
                     /gene="qacR"
                     /locus_tag="POB55_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001807342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug-binding transcriptional regulator
                     QacR"
                     /protein_id="HDI6501773.1"
                     /translation="MNLKDKILGVAKELFIKNGYNATTTGEIVKLSESSKGNLYYHFK
                     TKENLFLEILNIEESKWQEQWKKEQIKCKTNREKFYLYNELSLTTEYYYPLQNAIIEF
                     YTEYYKTNSINEKMNKLENKYIDAYHVIFKEGNLNGEWCINDVNAVSKIAANAVNGIV
                     TFTHEQNINERIKLMNKFSQIFLNGLSK"
     gene            complement(3175..3708)
                     /locus_tag="POB55_002891"
     CDS             complement(3175..3708)
                     /locus_tag="POB55_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDI6501774.1"
                     /translation="MIVGYARVSSNDQNLERQLENLKTFCAEKIFTEKQSGKSIENRP
                     VLQEALNFLRMGDRFVVESIDCLGRNYDEVVGNPLLDKFMKDLIIQILAMVSEQERNE
                     SKRRQAQGIQVAKEKGVYKGRPLLYSPNAKDPQKRIIYHRVVEMLEEGQSISKIAKEV
                     NITRQTIYRIKNDNDLI"
     gene            complement(4077..4922)
                     /gene="blaZ"
                     /locus_tag="POB55_002892"
     CDS             complement(4077..4922)
                     /gene="blaZ"
                     /locus_tag="POB55_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /protein_id="HDI6501775.1"
                     /translation="MKKLIFLIVMALVLSACNSNSSHAKELNDLEKKYNAHIGVYALD
                     TKSGKEVKFNSDKRFAYASTSKAINSAILLEQVPYNKLNKKVHINKDDIVAYSPILEK
                     YVGKDITLKELIEASMTYSDNTANNKIIKEIGGIKKVKQRLKELGDKVTNPVRYEIEL
                     NYYSPKSKKDTSTPAAFGKTLNKLIANGKLSKKNKNFLLDLMFNNKNGDTLIKDGVPK
                     DYKVADKSGQAITYASRNDVAFVYPKGQSEPIVLVIFTNKDNKSDKPNDKLISETAKS
                     VMKEF"
     gene            5029..6786
                     /gene="blaR1"
                     /locus_tag="POB55_002893"
     CDS             5029..6786
                     /gene="blaR1"
                     /locus_tag="POB55_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer BlaR1"
                     /protein_id="HDI6501776.1"
                     /translation="MAKLLIMSVVSFCFIFLLLVFFRYILKRYFNYSLNYKVWYLTVL
                     AGLIPFIPIKFSFIKFNNVNNQAPTVESKSHDLNHNIITTKPVQEFATDIHKFNWDSI
                     DNICTVIWIVLVIILSFKFLKALLYLKYLKKQSLYLNENEKNKIDTILFNHQYKKNIV
                     IRKAEAIQSPITFWYGKYIILIPSSYFKSVIDKRLKYIILHEYAHAKNRDTLHLIIFN
                     IFSIIMSYNPLVHIVKRKIIHDNEVEADRFVLNNINKNEFKTYAESIMDSVLKTSFFN
                     KNILSHSFNGKKSLLKNRLINIKEADLKKQSKLILIFICIFTFFIMIIQSQFLMGQSL
                     TDYNYKKPLQSDYQILDESKNFGSNSGSFVMYSMKKDKYYIYNEKESRKRYSPDSTYK
                     IYLALFGLDRHIISDKNSRMSWNHKHYLFESWNKEQDLNTAMQNSVNWYFERISNQIP
                     KNYTAAQLKQLNYGNENLGSYKSYWMEDSLKISNLEQVIVFKNMMEQNNHFSKKAKNQ
                     LSSSLLIKKNEKYELYGKTGTGIVNGKYNNGWFVGYVITNHDKYYFATHLSDGNPSGK
                     NAELISEKILKGMGVLNGQ"
     gene            6776..7156
                     /gene="blaI"
                     /locus_tag="POB55_002894"
     CDS             6776..7156
                     /gene="blaI"
                     /locus_tag="POB55_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillinase repressor BlaI"
                     /protein_id="HDI6501777.1"
                     /translation="MANKQVEISMAEWDVMNIIWDKKSVSANEIVVEIQKYKEVSDKT
                     IRTLITRLYKKEIIKRYKSENIYFYSSNIKEDDIKMKTAKTFLNKLYGGDMKSLVLNF
                     AKNEELNNKEIEELRDILNDISKK"
     gene            7420..8013
                     /locus_tag="POB55_002895"
     CDS             7420..8013
                     /locus_tag="POB55_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDI6501778.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNTYGCEKIFNDHMSGSKSKRPG
                     LDKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSST
                     GQLLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNQKDLNLLKTLYDNG
                     TPIKTIAEQWHVSRTTIYRYLNKLEDKEDEKQGEVSN"
     gene            7985..9428
                     /locus_tag="POB55_002896"
                     /pseudo
     CDS             7985..9428
                     /locus_tag="POB55_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790716.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            9421..10236
                     /locus_tag="POB55_002897"
     CDS             9421..10236
                     /locus_tag="POB55_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDI6501779.1"
                     /translation="MTKNQNFIETKEYKRFAEFCDACIKYQYIGICYGQPGVGKTLSS
                     RYYTNWDTIEKQVNHRGWEDLASKTTDDILSVDKIFYNAPAEKQTKLRNDLYSITAII
                     DLGQKLHVVNKYGHEHSKHYSDMFKYIDLIIVDEIDRLKVQHLEQLRAIYDEHNLAMI
                     FIGMPGIEKKLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKCQDLGFDLKLEDFTDY
                     EAITTIIKITKGNFRLIHRLFAQIDRIMDINGLDKISTEVVETARDSLVIGIR"
     gene            complement(10356..10733)
                     /locus_tag="POB55_002898"
     CDS             complement(10356..10733)
                     /locus_tag="POB55_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6501780.1"
                     /translation="MYVKTFYFQHTPFDDSYIEDIENYLLNYENNPHKSFMKIKVYDY
                     KNFLKHLKNILILIKQGKKPLAQAGHFKLSFYEEDLKLLQKNYKATLVDIKGLNKHFY
                     KECDRCLKAYNIFIEGINKHLYE"
     gene            complement(10805..11329)
                     /locus_tag="POB55_002899"
     CDS             complement(10805..11329)
                     /locus_tag="POB55_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HDI6501781.1"
                     /translation="MSNTKIKYQIFISSTYLDLIEERQAVVEAVLNSNNIPAGMELFN
                     SSSKSQWEIITQWINESDAVILLLGSSYGTIDSEENISYTEMEYNFAKSLGKPIYVFE
                     MNKASTESAEKSKKLDEFKTKVKENRLISPNINDISELKSKVSQTLIQSVPSEIKENS
                     GWIRVQDCGKNVKK"
     gene            complement(11541..>11623)
                     /locus_tag="POB55_002900"
     CDS             complement(11541..>11623)
                     /locus_tag="POB55_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HDI6501782.1"
                     /translation="FVHIIAPVISGCAVAYYTYWLSKRNK"
BASE COUNT         4280 a         1681 c         1652 g         4010 t
ORIGIN      
        1 cattagtagt atataattta aattttaatt ctcttgaaag gtctttgcta tattcgttat
       61 ttttataaac atcccatgtt gaaactatac tattataaat ttggttctgt tgcaaagtta
      121 aaattatatg cacatctata caaaactata aaagcaaata ttgttttaac agtaaagagt
      181 tttgttagta ttaattattt ttcgtttttc agtataaata ttacatgatt tttaactttg
      241 caacagaacc ttcaattcta tctttgtcac agattttgat tttactattt tttatttttt
      301 cctctaataa aataacctcg aacgatctgc taatttttaa taaatatgca ggaaaagcta
      361 atgttaagca taatgtaatt aattcattta cattgttcat gtaacttata aaatagatga
      421 aagttgctac tagtgctcct aaaaatatag ttatattaag ttctacatca aatattttaa
      481 aaatatctgc tctattcatc agttaatcat cctttcaagg tttattaata ctaataaatt
      541 attagatata ggtatatcat attattaatt taagaaattg tcttttataa ttttacttaa
      601 taataaaaaa gtagaaccat ttaaaattaa tggttctact ttttaactat tactaatttt
      661 aaaaataaac taatctacaa tatctaaaaa tatatgttta gtactatttt tgtttagtta
      721 tatcaagtga tttgggaatt aacaaataga caactattga aatgataatc atgataatcc
      781 cacctactaa agcagttgct acaaaagcat cattaaatga tgttacagcc tcgtttgcaa
      841 gctgttttat ccccgtagct ttagctactt cgacagcgcc cactacagat tcttcagcta
      901 catgagctaa atctccaact ataccttttg atgaaaaaga tgaaatatct aaaaatacac
      961 gataaagcat agaagatagg ctaccaagta ctgctacacc aaaaacattt ccaaggtcat
     1021 acatagactc ttcaacagca gctgcattac ctgcttttga tgtaggtgtt tctaacatta
     1081 ttagagcaga tgcaactgct agtgaagcca taccagctcc aactaaaatt aatgctaaag
     1141 ccattgtaga atatgataat ggatgaccaa agaaatacat aataaacatg ccaatggctg
     1201 caattccaat tccggaaggt aacactattt tcggtccaaa tcgcgccgct aatccgggtg
     1261 caattggtgc aaacaccata tctcctattg ccataggtaa taggtataag ccagctttaa
     1321 aaggagaaag ttcttccaca acctgtaacc attgtgaagc taataacaaa acagatgcca
     1381 ttgcaaacat tgtcataaat gcagcaattg taccagctga aaatgatctc tttttaaaaa
     1441 gtcttacgtc taacattgga tcagaacttg ataaattacg tttaacaaat atcactatca
     1501 tggtaattgc taatactatt acaacccatg gaataatatc tgctagtcct tcttttgaaa
     1561 attctttgat actccatacc agtccaatca tgcctgcaat tgataaaatt gtagaaggaa
     1621 tatcccacga gtgagacttt tcttttgata acttagactc tggtaataaa aataaacctg
     1681 ctacaactgc tattatcgca aacggtacat taattaaaaa tgccgagtgc catgaaaatt
     1741 gctcaagtaa agctcctccg ataattggtc caaaaacagc acctatcgat gaagcgattg
     1801 accatacagc taatgcagtg gccctttctt tagggttttc aaaaattact cttatcattg
     1861 aaagagtagt tggcattatt aaagcacctg caataccaag taaaaatcga atagctatta
     1921 cgaactctgc actttctgcg aaaaatatag ctaatgaaac gaggccaaat aaagcaaatc
     1981 cagttaataa tgcttttttt cttccccatt tatcagcaaa ggcactcaat ggaattataa
     2041 agccagctaa aacaagagag tatatatcaa ctatccataa ctgttgggta ccagaaggct
     2101 ctaactctct tactaattcc ggtaaagcca taattaatat tgtcatatcc attgtaacaa
     2161 caaacaaact aacagctaat actactagtg cagtccatct tttttttgat gtcatcatat
     2221 cagtagtttt tgtaaaaaat gaaatcattt aagttccctc caatccttat agaccgtgcg
     2281 atcggtctat aaggattata atctattttt tttacatatt acaacaaaaa atcttgcgaa
     2341 atttaaatgg ctgtattgtg ttatacaatt attctttgaa ttttttgtgc tatcattgat
     2401 agtacttaat actcattaaa ggcgtgatga acttgaaaga taaaatacta ggtgtcgcaa
     2461 aggaattatt tataaaaaat ggatataatg ccactactac tggagaaatt gttaaattat
     2521 cagaaagtag taaagggaat ctttattatc actttaaaac aaaagaaaat ctatttttag
     2581 aaattttaaa tatagaagaa tctaaatggc aagaacagtg gaaaaaggaa caaatcaaat
     2641 gtaaaactaa tagagaaaaa ttttatttat ataatgaact atctttaaca accgaatact
     2701 attatccact tcaaaacgca ataattgaat tttacactga atattataaa actaatagca
     2761 ttaatgaaaa aatgaataaa ttagaaaaca aatatataga tgcttatcat gtaattttta
     2821 aggagggtaa tttaaatggc gaatggtgta ttaatgatgt taatgctgtt agtaaaatag
     2881 cagcaaacgc tgttaatgga attgttacct ttacacatga acaaaatatt aatgaaagaa
     2941 ttaaacttat gaacaagttc tctcaaattt ttttaaatgg acttagtaaa taattatata
     3001 tatattcttg aaataccaat aaatattatt tctaaattac gttcgttaat gagaaaaaga
     3061 gagagcatat ttacgctctc ttttcttgtc ttattattaa aaactcataa tagattataa
     3121 tcatctgttt tgagacgctg tgagaagctc tcagttgcat tgaaacaaag tttattaaat
     3181 caaatcatta tcgtttttaa ttctgtaaat cgtctgcctt gttatattta cttctttcgc
     3241 aattttacta attgattggc cttcttctaa catttctaca acgcgatgat aaataatacg
     3301 tttctgtgga tctttagcat ttggtgaata aagtaagggg cgccctttat atacgccttt
     3361 ttccttcgca acttgaattc cttgcgcttg tcgacgttta ctttcatttc tctcttgttc
     3421 tgaaaccatt gctaatattt gaataattaa atctttcata aacttatcca gtaatggatt
     3481 gccaaccact tcatcataat tacgacctaa acaatcaatg gattctacta caaaccgatc
     3541 tcccatcctc aaaaagttaa gtgcttcttg aagtactggt ctattttcaa tagatttccc
     3601 agactgtttt tctgtaaata ttttttcagc acaaaacgtc tttaaatttt caagttgtct
     3661 ttctaaattt tgatcgttcg atgatactct cgcatagcca acaatcattg atttccatct
     3721 cataattaat atacacccta atcatacgtt tcagttacta taaattcaac aattatgtca
     3781 ctttgcttga tatatgagaa ttatttaacc ttataaatga gaaaaaagca acgcacttta
     3841 aataagatac gttgcttttt cgattgatga acacctataa ttaaactatt catctattat
     3901 ttatgatttt ttgtatatac aatatttcta gtttgttaaa gagaattaag aaaataaatc
     3961 tcgaaaataa taaagggaaa atcaggtttt gatatcaaaa ttatacatgt caacgataat
     4021 agaaaatata atacaaacta taagatgtta tcagtattta ttatgcattt agaatattaa
     4081 aattccttca ttacactctt ggcggtttca cttatcaact tatcatttgg cttatcactt
     4141 ttattgtctt tattcgtaaa aatgactaaa acaataggtt cagattggcc cttaggataa
     4201 acaaaagcaa catcatttct agaagcatat gttattgctt gaccactttt atcagcaacc
     4261 ttatagtctt ttggaacacc atctttaatt aaagtgtctc cgtttttatt attaaacatt
     4321 aaatcaagta agaaattttt attttttttg cttaattttc catttgcgat aagtttattt
     4381 aaagtcttgc cgaaagcagc aggcgttgaa gtatcttttt tgctctttgg cgaatagtaa
     4441 tttaattcta tctcatatct aactggattt gttactttat ctcctagttc ttttagacgt
     4501 tgtttaactt ttttgattcc accgatttct tttataattt tattgtttgc tgtattatca
     4561 ctatatgtca ttgaagcctc aataagttct tttaaagtga tgtcttttcc tacatatttt
     4621 tctaaaatag gagaataagc aactatatca tctttgttaa tatgtacttt tttatttaac
     4681 ttattataag gtacttgttc taacaaaata gcactattta tcgcttttga agtcgaagca
     4741 taggcaaatc tcttatctga attaaatttt acttccttac cacttttagt atctaaagca
     4801 taaacaccaa tatgagcatt atattttttt tctaaatcat ttaactcttt ggcatgtgaa
     4861 ctgtttgaat tacatgcact taaaactaaa gccattacaa ttaaaaatat taactttttc
     4921 aaaataaacc ctccgatatt acagttgtaa tttttacaaa acaataatat tacaattgta
     4981 atattgatgt caattgttta ttataaatat ttgaaagaag gtgtcaaaat ggctaaatta
     5041 ctaataatga gtgtagtaag cttttgtttt atatttctat tgttagtctt ttttaggtac
     5101 atattaaaac gctattttaa ttatagttta aattataaag tttggtatct aactgtgctt
     5161 gctggattaa ttccttttat cccaattaaa ttctctttta taaaatttaa taatgtgaat
     5221 aatcaagcgc ccacagttga aagtaagtca cacgacttga accataacat aattaccacc
     5281 aaacctgttc aagagttcgc aacagatatc cataagttta attgggattc aattgataat
     5341 atctgcacag ttatttggat agttttagtt attattttaa gttttaaatt tttgaaagcc
     5401 ttattatatc ttaaatattt aaagaaacag tcactttatc taaacgaaaa tgaaaaaaat
     5461 aaaatagata cgatactttt caaccatcaa tataaaaaaa atattgtgat tcgaaaagca
     5521 gaggctattc aatctccaat aactttttgg tatgggaaat atattatttt gattcctagt
     5581 tcatatttta aaagtgtaat tgataaaaga ctaaaatata taatactgca tgaatatgct
     5641 catgctaaaa atagagatac tttacattta attatcttta atatcttctc tattattatg
     5701 agttacaatc cattagtaca tattgtaaaa agaaagataa ttcatgacaa tgaagtagaa
     5761 gccgatagat ttgtccttaa taatattaac aaaaatgaat ttaaaactta tgcggaatct
     5821 attatggact ccgtattaaa aacctcattt ttcaacaaaa atatattaag ccattcattt
     5881 aatggtaaaa aatcattact caaaaataga ttaattaata taaaagaagc cgatctgaaa
     5941 aagcagtcaa aattaattct aatttttatt tgtattttta ctttttttat catgataatt
     6001 caaagccagt ttttaatggg gcaatcctta actgattata attataaaaa gcccttacaa
     6061 agcgattacc aaattttaga tgaaagtaaa aattttggtt caaatagtgg atctttcgtt
     6121 atgtacagca tgaagaaaga caaatactat atttataatg aaaaagaaag tagaaaaaga
     6181 tattctcctg attcaactta caaaatttat ttagcactgt ttggacttga ccgtcatatt
     6241 ataagtgata aaaattcacg tatgtcatgg aatcataaac attatctttt cgagtcttgg
     6301 aataaggaac aagatttaaa tacagcaatg caaaattcag ttaattggta cttcgaacgt
     6361 attagtaatc aaataccaaa gaactatact gcggctcaac tcaagcaatt aaattatggt
     6421 aatgaaaatt tgggaagtta taaaagctat tggatggaag atagtttgaa aatatctaat
     6481 cttgaacaag taatagtttt taaaaatatg atggaacaaa ataaccattt tagtaaaaaa
     6541 gcaaagaatc aattatcttc ttcattattg attaagaaaa atgaaaagta tgaactgtat
     6601 gggaaaacag gtacaggtat agtaaacggg aagtataata atgggtggtt tgtaggttac
     6661 gtaattacaa atcatgataa gtattatttt gctacacatt tatcagatgg aaacccgtct
     6721 gggaaaaatg ctgaattaat aagtgaaaaa atattaaaag gaatgggtgt tttaaatggc
     6781 caataagcaa gttgaaatat ctatggctga atgggatgtt atgaatataa tatgggataa
     6841 aaaatcagta tcagctaatg aaattgtagt tgaaattcaa aaatataaag aagttagtga
     6901 taaaacgatt agaacattaa tcacaagact atataaaaaa gagattataa aacgatacaa
     6961 atcagaaaat atttattttt actcatcaaa tattaaagaa gacgatatta aaatgaaaac
     7021 tgctaaaacc tttcttaata aactgtatgg aggggatatg aaaagtttag tgttgaattt
     7081 tgcgaaaaat gaagaattaa ataacaaaga aattgaagaa ttgcgagata ttttaaatga
     7141 tattagtaaa aagtaattag aggtatgttt caaacataat acatatgtaa actatgcttg
     7201 actcttatta aattagtttt gttaatgaca tttaaaatat attattaaag atgagagtgt
     7261 acagaaaata accattttct gtacactctt cttttatgtc taaataagta agaattacat
     7321 aggattatgt gttcaaaaac ccaacaaaaa atctatgttc ataaaagacc ttgtaattaa
     7381 gttatggtac actgaagatg tatataggag gtttgtattt tgaaaatagg ttatgcaaga
     7441 gtttcaactg gattacaaaa tttaaattta caggaggatc gattaaatac atatggttgt
     7501 gaaaagatat ttaatgacca tatgagtggt tcaaaaagta aaagacctgg tttagataaa
     7561 gcaattgaat ttgcgaggtc aggagatacg attgttgttt ggagattaga tagattggga
     7621 cgtaatatgg aggatttaat cacattagtt aatgagctta acgaacgagg tgtaagtttc
     7681 catagtttag aagaaaatat aacaatggat aaatcaagtt caactggaca attattattt
     7741 catttattcg cagcatttgc agaatttgaa cgtaatttaa ttttagagcg ttcttcagct
     7801 ggccgaattg cagcacgtgc tagaggccga tatggaggca gaccagaaaa attaaatcaa
     7861 aaagatttaa acttacttaa gaccctttat gataatggca cgccaattaa gacaattgca
     7921 gaacagtggc atgtttcacg tacaacaatt tatcgttacc tcaataaatt ggaggacaag
     7981 gaagatgaaa aacaaggaga agtatctaac taatttttct gaagccaaac gtaaaaaagc
     8041 tactcaaaag tacaatatta taaaaccttt tatcctaggc gaacaatctt tatcaagtat
     8101 ttctaaaagt aaagggattg cgttaagcac cctttatagg tggaataagt catataaaga
     8161 acaaggactt aaaggactaa tctatgcgac aagagctgat aaaggaacac gtaaaataga
     8221 accaaagata attgatgaaa ttgaacgcct tgcgcttatg aataaaagaa atagtatagc
     8281 cacaattcat aggaaaatca ctaattactg taaagaaaat aattttgata taccaagtta
     8341 taaacaaatt tatagtgtta ttaaagcgat gcctaagtct gttattgatt tctctcatca
     8401 gggtgaaaaa tactatcaaa ataagtacga tttaatacaa attagagaat cctcgagacc
     8461 caatgaaata tggcaagcag accatacttt gctagatatc tttatattag accaaaaagg
     8521 taatatcaat agaccatggc taactattat tatggatgac tatagtcgtg caattgcagg
     8581 ctatttctta agttttgatg ccccccaatg ctcaaaacac agcattaaca ctacaccaag
     8641 caatatggaa taaaaatgat accaattggc cagtgtgtgg tatacccgaa aaattctaca
     8701 cagatcatgg aagtgacttt acttcacatc atatggaaca agtcgctatt gatttgaaaa
     8761 ttaacttgat gttttcgaaa gttggtgttc ctcgtggtcg tggaaaaata gaacgattct
     8821 ttcagacagt taatcaaact ttcttagaac aactgcctgg atatataaac aataatgata
     8881 ctcctagtaa tctaataaat tttcacaatt ttgaagaaaa attacgttac tttttaattg
     8941 aagattataa tcaaaaagaa catagtacta ttcataccac gcctatcagt cattggaata
     9001 gtaatcactt tttccctaat atgccaagta gcttagaaca acttgattta ttattgttgg
     9061 aaatacctaa atctagaaaa gttcattcag atggcattca ttttcaaggt tttcgataca
     9121 gtaatactaa tctagaagct tatgtaggtg aatatgtact gattcgttat aacccaaatg
     9181 atatggctga aatacgtgtg ttttatagag atgaatttct ttgtactgct atatgtccag
     9241 aaatagctga ttactccata gatataaaag acatacagca cgctcgtagt cagagaagaa
     9301 aacacttaaa acaaaatatt gctagtccaa gtaccacgga attaattaag gaagaaaaaa
     9361 attgtggtta ttcacctcaa gaaacgacta aaaacgtcaa aaaattaaag agatatcgta
     9421 atgactaaaa atcaaaattt cattgaaacg aaagagtata aacgctttgc tgaattttgt
     9481 gatgcttgta ttaaatatca gtatattggt atatgttatg gtcaacctgg tgtcggaaaa
     9541 actttatcgt ctaggtatta cacaaattgg gatactattg aaaaacaagt taaccataga
     9601 ggttgggaag atttagctag taaaacgacc gatgacatac tatcggtgga taaaatattt
     9661 tataatgcac cagcagaaaa acaaactaaa ttaaggaatg atttatatag tattactgca
     9721 atcattgatt tgggtcaaaa attacatgtt gtaaataagt atggacatga acatagtaaa
     9781 cattatagtg atatgtttaa atatattgat ttaattatag tagatgagat tgatcgcctt
     9841 aaagtgcaac atttagagca attaagagct atttatgatg aacataattt agcaatgata
     9901 tttattggta tgccgggtat agagaaaaaa ctatctcgtt atcctcaact atattcgcgt
     9961 ataggttttg cgcatgaatt tgataatcta agtaaagatg agacgcatca tatattagaa
    10021 tataagtgtc aagacttagg atttgattta aaactcgaag actttactga ttatgaggca
    10081 ataacgacga ttattaaaat tacaaaaggg aattttaggc tgattcatcg tttatttgcg
    10141 cagatagaca gaattatgga tataaatggt ttagataaaa taagtacaga agttgtagaa
    10201 acagctagag atagtttagt tataggtatt cgataataga aaaagcaacg cattttaata
    10261 tagtgcgttg cttttctcat ttattaggta aagtcattct catatatcga gtaaagtgac
    10321 aacaattata tatgtatgat tagggtgtac ttatatcatt catataaatg tttatttata
    10381 ccctcaataa aaatattata tgcttttaag cacctatcac attccttata aaaatgttta
    10441 ttcaatcctt taatatcgac tagcgtagcc ttataatttt tttgtaacaa ttttaaatcc
    10501 tcttcgtaaa agcttaattt aaaatgaccg gcttgagcta atggcttttt tccttgttta
    10561 attaaaatca atatgttttt taaatgtttt aaaaagtttt tataatcata aacttttatt
    10621 ttcatgaatg atttatgtgg attattttca taatttagca ggtagttttc tatatcttca
    10681 atataactat catcaaatgg tgtgtgctgg aaataaaaag tttttacata catgaatatt
    10741 ttataaagtt cttctttcaa aaaatcatat gtatctaaat ctctttgtat agcatccaat
    10801 ttttttattt ttttacattt ttaccacaat cctgtactct tatccatcca ctattttctt
    10861 taatttcact aggtacactt tgtattaaag tctgagatac tttacttttt aattccgaaa
    10921 tatcattaat atttggacta attagtctat tttctttaac ttttgtctta aactcatcta
    10981 attttttaga cttttccgcg gattcggtag atgctttatt catctcaaaa acatatatag
    11041 gtttacctaa agatttagca aaattgtact ccatttctgt atagctaata ttttcttcac
    11101 tatcaattgt tccataacta gaacctaaca ataatatcac tgcatctgat tcattgatcc
    11161 attgtgttat tatttcccat tggcttttac tacttgagtt aaaaagttcc atacctgctg
    11221 gaatattatt actgtttaaa actgcttcta ctactgcttg acgctcttct attaaatcta
    11281 aataagttga tgaaataaaa atttgatatt taattttagt attactcata tatgctcctt
    11341 taataaaaaa tcaatttaca taaatatatc atatgataat atccaaaaag actacttaaa
    11401 atattatctt tttgcattcc tctactaacc agtgttataa tctagataac gaaaatgatt
    11461 aacaattaat gcaccaatcc ccacactact gcaatagtgt ggggattttt tcgatgctgg
    11521 cttataaagt cgcctattaa ttatttgttg cgtttactaa gccaataagt ataatacgca
    11581 acagcacagc cactgatgac tggtgctatg atgtgaacga aaa
//
LOCUS       DAMZKC010000423         6774 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.guided.131, whole genome shotgun sequence.
ACCESSION   DAMZKC010000423 DAMZKC010000000
VERSION     DAMZKC010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6774
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.guided.131"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            168..3241
                     /gene="mupA"
                     /locus_tag="POB55_002901"
                     /pseudo
     CDS             168..3241
                     /gene="mupA"
                     /locus_tag="POB55_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163435.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mupirocin-resistant isoleucine--tRNA ligase
                     MupA"
     gene            3311..4198
                     /locus_tag="POB55_002902"
     CDS             3311..4198
                     /locus_tag="POB55_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HDI6501783.1"
                     /translation="MYNFDYSKLPIKNIQKIFPIAGGYVNLSFSVDASNKKYFLKLQP
                     NTKSNFFDYELSSLKELTDKNIPVPQIINKGELDNNSFLLLEFIENGHAYPESYRKLG
                     KIVANMHKNINSLNLFGFSHNFNGGTIEFTNKWTSSWGDLFIKSRMDKLCSEIYKKRL
                     FSISDLLLYEDIRKIMLKSLYYHQSSPSLLHGDLWKGNILFQKNGDPILCDPVCLYGD
                     REFDIGYTLALEKFPLDFYQEYNNIYPLCVGYEKRIEFYKLYVFMIHLFAIGTDFYDK
                     VKDTMYNILNNYGVDFIKG"
     gene            4543..4836
                     /locus_tag="POB55_002903"
     CDS             4543..4836
                     /locus_tag="POB55_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000726503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HDI6501784.1"
                     /translation="MKKKFVSSCIASTILFGTLLGVTYKAEAATVHVAGGVWSHGIGK
                     HYVWSYYSHNKRNHGSTAVGKYSSFSGVARPGVQSKASAPKAWGGNKTFYSLH"
     gene            4911..>6774
                     /locus_tag="POB55_002904"
     CDS             4911..>6774
                     /locus_tag="POB55_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001670421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HDI6501785.1"
                     /translation="MKKFKLFLDFSTLLLISGLLFLFFFKENEEIIPESSNILTISNW
                     DKSNSKSKVLDVIESGAKNQNIQIIKSVKDFDNKKEFFVFNSKRNNSDFIRNKTSLLT
                     PSDLLNREIKGKYYIIGEHFNVEELTSELKKAGLTSEVEYINRNILFFELVIDNDLLV
                     PFIFLGVLYLLYFLYDRGYNLKFYAIQKLHGFSTTRIIFHNIHIKIIYWLVLTAIFFI
                     ANILIIHIANLELDFLMFIRRFIVLLFVFACITTLLWLISYSLLLKLSIPLILKGKKG
                     YKFSIFMGTFTKCIMVLILSFLLAQNLNAYTKLKNIYDSEKIWQKLDNYYTLEISPNI
                     RNSEEKQILETNIYNLIKKSEQLGGLLLKNNNIANPDKNNYIPENGNVFFINNNFLNF
                     YKNINHDFKMIDNHSDKINVFIPPRLQYQKSEIQKNHQGWIDFQKQQNKKVDVQVLSK
                     NVSVFSFDRTTNLKFGYLDSPIVMLLTLDDLTGDFYLAAVSQGGYLFKDLDTLKQLLK
                     DNHLEEEISGITNYKDSVLNELNETKTKMIITIVTIIINVFILLIATVFETIQYFSWN
                     KKQLLLRKIHGYSLFSSNVRYLTISILLSIMLAYTTHILFGSKILLFIIMSIAII"
BASE COUNT         2573 a          836 c         1027 g         2338 t
ORIGIN      
        1 aaattatata ctactaatga tataatttgc agtagtttgt attataactt tactttatcc
       61 aatatatctt tctatggaga catgaaagga aatgaatttc atgaaaattc accaagacta
      121 atagtaaaaa taacgaaata agtgatactc taggaggctg aaaagttttg acaaagaaat
      181 atttaaacac ccagaatgaa atatcagcat tttggaatac tcaaaagata tttaaaaaat
      241 caattgacaa tagaaaagga caggaaagtt ttgtttttta tgacggcccc ccaactgcaa
      301 atggccttcc tcatgctggc catgttcttg gaagagtaat caaggattta gttgcaagat
      361 taaaaactat gcaaggtttt tatgtagaaa gaaaagcagg atgggatacc catggcttac
      421 cagttgaatt agaggttgaa aaaaaattgg aattaaagga aaacaagaca ttgaaaagta
      481 tggaatagaa aattttataa atgaatgtaa aaaaagtgta tttaattatg aaaaagaatg
      541 gcgggatttt tctaaagatt taggatactg ggttgacatg gactccccct atataactct
      601 tgagaataat tatattgaaa gtgtatggaa tatattatct acattccata aaaaaggact
      661 attatataag ggacataagg tgactcctta ttgtacacat gatcaaaccg ctttaagttc
      721 tcatgaagta gcgcaaggct ataaaaacgt taaagattta tcagctgttg ttaaatttca
      781 acttacaaat agtaaagata cttatttctt aagttggact accactccct ggactttgcc
      841 tgcaaatgta gcattagcta taaataaaga tcttaattat tcaaaaattc gggtagaaaa
      901 tgagtattat atcttagcta cagatctaat taattctata ataactgaaa aatacgaaat
      961 tattgatacc ttttcaggaa gtaatttaat taatttaaaa tacattcctc cttttgaaag
     1021 cgacggttta gttaatgcat attacgttgt tgatggagaa tttgttacta actcagaagg
     1081 aactggtatt gttcatatag caccagctca tggggaagat gactaccaat tggttttaga
     1141 gcgtgatttg gatttcttaa atgttataac aagagaagga gtatataatg ataggttccc
     1201 tgaattagtt ggtaataaag ctaaaaatag tgatatagaa atcataaaat tattatccaa
     1261 aaaacaactt ttatataaaa aacaaaaata tgagcataat tatcctcatt gttggagatg
     1321 tggtaatcct ttgatatatt atgcgatgga aggttggttt attaaaacaa ctaattttaa
     1381 gaatgaaatt attaacaata ataataatat agagtggttt ccttctcata ttaaggaagg
     1441 gagaatggga aatttcttag aaaatatggt tgattggaac attggtagaa atagatattg
     1501 gggaacacca ttaaatgtat ggatttgcaa tgattgtaat cacgaatacg caccaagtag
     1561 tattaaggat ttacaaaata attccatcaa taaaattgat gaagatattg agttgcatag
     1621 accttatgtt gataatatca ctcttagttg ccctaagtgt aatgggaaaa tgtctcgagt
     1681 agaagaagta atcgatgttt ggtttgatag cggctctatg ccgtttgctc agcatcatta
     1741 tccttttgat aaccagaaaa tttttaatca acactttcca gctgatttta ttgcagaagg
     1801 agttgatcaa acgagaggct ggttttacag tttactagta atttctacta ttctaaaagg
     1861 aaaatcttct tataaacgtg ctttatcttt aggacatatt ctagacagta atggtaaaaa
     1921 aatgtctaaa agtaaaggaa acgttattaa tccaactgaa ttaattaata agtacggagc
     1981 cgattcttta agatgggcct taatttcgga tagtgctcca tggaataaca aaagattctc
     2041 agaaaatata gtagctcaga ccaaatcgaa atttatagat acgcttgata atatttataa
     2101 attttataat atgtataata aaatagatca ctataatcct aataatgaaa ttacaaaaag
     2161 tagaaataca ttagataatt gggctctttc tcgcttaaac accttaataa aagaaagtaa
     2221 tatttatgta aataattacg atttcacttc cgcagccaga ttaattaacg aatataccaa
     2281 tacaataagt aattggtata ttcggagatc gagaggacga ttttgggaac aaggaatttc
     2341 taacgataaa aaagatgcgt acaatacgct ttatgaaatt ttaacaactt tatcaagact
     2401 agtggctcca tttgttccat ttatatctga aaaaatccat tataatttga ctggaaaaag
     2461 tgtgcattta caagattatc cacaatataa agaaagtttt attaatcaag cattggaaga
     2521 tgaaatgcat accgttataa aaattgtaga attatctaga caggctcgca aaaatgcaga
     2581 tttaaaaatt aagcaacctt tatcgaaaat ggtgattaaa cctaatagtc aattaaactt
     2641 aagtttttta cctaattact attcaataat aaaagacgaa ttaaatataa aaaacattga
     2701 attaactgat aatattaatg actatattac ctatgagctt aaattgaatt tttcttctgt
     2761 gggaccaaaa ctagggaaca aaacgaaaaa tattcaaaca ttgatagact ccctatcaga
     2821 gtatgataaa aaaagtttaa ttgagtctaa taacttcaaa agtttatctt ctgatgctga
     2881 gttaactaag gatgatttta taattaaaac cttacctaag gatagttatc aactcagtga
     2941 agataatgac tgcgttatat tattagataa aaatttatct cctgaattaa ttcgcgaagg
     3001 acatgctaga gagctcatta gattaattca acaattaaga aaaaagaaaa atttaccaat
     3061 aaatcaacgt attgatattt atatcggtgt aactggggaa ttattagaat caataaaaac
     3121 caataaaaat atgtttaaag aaaatttgct gattaaaaat atacacttaa atgttataga
     3181 tgaatatgaa aatactattc attttaataa taaagaaata aaaatttcct tattatatta
     3241 aacaaacatg gccactctat tttagtagag tggccatgag ttatcaaagc caagcaaaag
     3301 gtgataaact atgtataatt ttgattattc taaattacca attaaaaata ttcaaaaaat
     3361 atttcctatt gcagggggat atgtgaatct atctttttca gttgatgctt ccaacaaaaa
     3421 atatttttta aagctacaac ctaatactaa atcaaatttt tttgattatg agttatccag
     3481 tttaaaagaa ttaactgata aaaatattcc tgttcctcaa attattaata aaggagaatt
     3541 agacaataat tcttttttac tgttagagtt cattgaaaat ggccatgctt atcctgaaag
     3601 ctatagaaaa ctaggtaaaa tagttgcaaa catgcataaa aatataaatt cactaaatct
     3661 atttggcttt agtcataatt tcaatggagg aacgattgaa ttcactaata aatggacttc
     3721 ttcttggggg gatttattta ttaaaagtag gatggataag ctatgttctg aaatatacaa
     3781 gaaacgttta ttttcaattt cggatctttt attatatgaa gatatacgaa aaattatgtt
     3841 gaagagctta tattatcacc aatcgtcacc atcattactt catggtgatc tttggaaagg
     3901 aaatattctt tttcaaaaaa atggagatcc tattctatgt gatccagtct gtttatatgg
     3961 agatagagaa tttgatattg gatatactct agcattagaa aaatttccat tagattttta
     4021 tcaagaatat aataatatat atccgttgtg tgttggatat gaaaagcgaa tagaatttta
     4081 caagttatat gtgtttatga ttcatttatt tgctataggt acagattttt atgataaagt
     4141 taaagatacc atgtataata ttttaaataa ttacggggtt gattttataa aaggataggg
     4201 tgttgacttt gtttcaattt tctaaaatca tttatctact tctaatagtc acttttttcg
     4261 aacgtttata acgcaatcat gatgagagta ttaatacagt taattatttg aaatacttta
     4321 gtttatataa tgttgaatta taggtagtga aattgtattt attatacatt gtcatatcgt
     4381 tgtttaatga tagttaattt aaggataata gaattaatat tatatataga tgaacaaaac
     4441 aagcaacaga gcagaaaaaa cacttttaga ttatttttac tcattctcta ttttatttat
     4501 ttttgctata ataaataaat aatattaaat ggaggtgttt ttatgaaaaa gaaatttgtt
     4561 tctagttgta ttgctagtac aatcttattc ggcactttat taggtgtaac ttataaagct
     4621 gaagctgcaa cagtacatgt agctggtggt gtttggagcc atggtattgg aaaacattac
     4681 gtatggtctt attatagtca caataaaaga aatcatggtt caacagcagt agggaaatat
     4741 tcatctttta gtggtgttgc tcgacctggt gttcaatcta aggcatctgc tccaaaggct
     4801 tggggtggaa acaaaacatt ttatagtctt cactaattac atatattaat taaggagaat
     4861 agacagaatt aagtaaactt aattctgtct attctcttga aagaggcaat atgaaaaaat
     4921 tcaaattatt tttagatttt agtactttat tattaatttc agggctatta tttttatttt
     4981 tcttcaaaga aaatgaagaa attatacctg aaagttccaa tattttaaca atctctaatt
     5041 gggataaatc taacagtaaa tcaaaagtgt tagatgtcat tgaatcaggc gccaaaaatc
     5101 aaaatataca aataattaaa tccgttaaag attttgataa taaaaaagaa ttttttgttt
     5161 tcaactcaaa acgaaataat tctgacttta tacgtaataa aacatcactt ttaactccat
     5221 cagatttatt aaacagagag ataaaaggaa agtattatat tattggtgaa catttcaatg
     5281 tagaagagct tacgagtgaa ttaaaaaaag ctggtttaac atctgaagtt gaatacataa
     5341 atagaaacat attatttttt gaattagtca tagataatga tttattagtg cctttcatat
     5401 tcttaggcgt attatatctt ttgtacttct tatatgacag aggatataat ttgaaatttt
     5461 atgctattca aaagctacat ggcttttcaa ctacacgcat tatttttcat aatattcata
     5521 taaaaatcat ttattggcta gtacttaccg ctattttctt tattgctaat atattaatca
     5581 ttcatatagc caatttagaa ttggatttct taatgtttat tcgtagattt atcgtgttgc
     5641 tttttgtttt tgcttgtatt actacattgc tgtggctgat ttcgtatagt cttcttttga
     5701 agttaagtat tccattaatc ttaaaaggga aaaaaggata taagttttca atatttatgg
     5761 gtaccttcac caaatgtatc atggtgctaa tactatcttt tttactggca caaaatttaa
     5821 atgcttatac gaagttaaag aatatatatg actctgaaaa aatctggcaa aagttagata
     5881 attactatac attagagatt tctcctaata ttagaaattc agaagaaaaa caaattttag
     5941 aaactaatat atacaattta attaagaaaa gcgaacaact tggtggattg cttttaaaga
     6001 ataataatat agccaatccc gataaaaaca attacattcc tgaaaatggc aatgtctttt
     6061 ttatcaataa caattttctg aatttctata aaaatataaa tcatgatttt aaaatgattg
     6121 acaatcattc ggataaaata aacgtgttta ttcctccaag gttacaatat caaaaatctg
     6181 aaattcaaaa aaatcaccaa ggatggattg actttcaaaa gcaacaaaac aaaaaagtag
     6241 atgtgcaagt actgtcaaaa aacgtatctg ttttttcttt tgatcgcaca actaatttga
     6301 aatttgggta tctagattct ccaatagtaa tgttgttgac acttgacgat ttaacaggag
     6361 atttttattt agctgctgtt tctcaaggtg gatatctatt taaagattta gatacactta
     6421 aacagttgct taaagataat cacctagaag aagaaattag cggtataact aattacaagg
     6481 attctgtgtt aaatgaatta aatgaaacga aaacaaaaat gataataaca atagttacta
     6541 ttattattaa cgttttcatt cttttgatag ctactgtatt tgaaacaatt caatatttta
     6601 gctggaataa aaagcaacta ttattaagaa aaattcatgg ttattcatta ttcagcagta
     6661 atgtacgtta tttaacaatt tctatcttac tttctattat gttagcttac actacgcata
     6721 ttttatttgg ttcaaagata ttactattca taataatgtc tattgctatt attc
//
LOCUS       DAMZKC010000424         4173 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.guided.184, whole genome shotgun sequence.
ACCESSION   DAMZKC010000424 DAMZKC010000000
VERSION     DAMZKC010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4173
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.guided.184"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            59..784
                     /gene="sasD"
                     /locus_tag="POB55_002905"
     CDS             59..784
                     /gene="sasD"
                     /locus_tag="POB55_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070002951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-wall-anchored protein SasD"
                     /protein_id="HDI6501786.1"
                     /translation="MKKLATVGSLIVTSTLVFSSMPFQNAHADTTSMNVSNKQSQNVQ
                     NHRPYGGVVPQGMTQAQYTELEKALSQLSAGSNMQDYNMKLYDATQNIADKYNVIITT
                     NVGVFKPHAVRDMNGHALPLTKDGNFYQTNVDANGINHGGSEMVQNKTGHMSQQGHMN
                     QNTHMNQQPHMQQGHMQSSNHQMMSPKANMHSSNHQMNQSNKKVLPAAGESMTSSILT
                     ASIAALLLVSGLFLAFRRRSTNK"
     gene            complement(975..1730)
                     /locus_tag="POB55_002906"
     CDS             complement(975..1730)
                     /locus_tag="POB55_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDI6501787.1"
                     /translation="MEVVYVTQTQPKFLTIYNTLYKEIQIGKYPSGHALPTEKELCAR
                     FDVSRMTLRQAIKLLAEDGIVESTRGKGHFVIPQTNAQHASSMDQFKHPLDQILLARV
                     SMSSINYRVDLESEYTNHLFANHPSAVIAMERYYQKKDNHSKQADAFCFTFIPLNVID
                     TFKVNTQNEDDMKTFVEQTIYSNAYQSDLKMSITKAPHFKNHSHVFDGDTHCWLIIET
                     LYAQTPYPIMINKWYIPQEISELTLTRIRQSDY"
     gene            1966..2673
                     /gene="deoD"
                     /locus_tag="POB55_002907"
     CDS             1966..2673
                     /gene="deoD"
                     /locus_tag="POB55_002907"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDI6501788.1"
                     /translation="MKSTPHIKPMNDVEIAETVLLPGDPLRAKFIAETYLDDVEQFNT
                     VRNMFGFTGTYKGKKVSVMGSGMGMPSIGIYSYELIHTFGCKKLIRVGSCGAMQENID
                     LYDVIIAQGASTDSNYVQQYQLPGHFAPIASYQLLEKAVGTARDKGVRHHVGNVLSSD
                     IFYNADTTASERWMRMGILGVEMESAALYMNAIYAGVEALGVFTVSDHLIHETSTTPE
                     ERERAFTDMIEIALSLV"
     gene            2680..4032
                     /gene="tet(38)"
                     /locus_tag="POB55_002908"
     CDS             2680..4032
                     /gene="tet(38)"
                     /locus_tag="POB55_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(38)"
                     /protein_id="HDI6501789.1"
                     /translation="MNVEYSKIKKAVPILLFLFVFSLVIDNSFKLISVAIADDLNISV
                     TTVSWQATLAGLVIGIGAVVYASLSDAISIRTLFIYGVILIIIGSIIGYIFQHQFALL
                     LVGRIIQTAGLAAAETLYVIYVAKYLSKEDQKTYLGLSTSSYSLSLVIGTLSGGFIST
                     YLHWTNMFLIALIVVFTLPFLFKLLPKENNTNKAHLDFVGLILVATIATTVMLFITNF
                     NWLYMIGALIAIIVFALYIKNAQRPLVNKSFFQNKRYASFLFIVFVMYAIQLGYIFTF
                     PFIMEQIYHLQLDTTSLLLVPGYIVAVIVGALSGKIGEYLNSKQAIITAIILIALSLI
                     LPAFAVGNHISIFVISMIFFAGSFALMYAPLLNEAIKTIDLNMTGVAIGFYNLIINVA
                     VSVGIAIAAALIDFKALNFPGNDALSSHFGIILIILGLMSIVGLVLFVSLNRWTQSEK
                     "
BASE COUNT         1382 a          651 c          744 g         1396 t
ORIGIN      
        1 ttttgtaatc gtatgataat gtaaatgtaa tcaaattgta atataagggg acaagacaat
       61 gaaaaaatta gcaacagtag gttctttaat tgtaacaagc actttagtat tctcaagtat
      121 gccttttcaa aatgcgcatg ccgacacaac ttcaatgaat gtgtcgaata aacaaagcca
      181 aaatgtacaa aatcatcgtc cttatggcgg agtagtacca caaggaatga cgcaagcaca
      241 atatactgaa ttagagaaag ctttatccca attaagcgct ggcagtaata tgcaagacta
      301 taatatgaaa ttgtatgatg cgacgcaaaa tattgctgat aaatacaatg tgataattac
      361 aactaatgta ggggtattta aaccacatgc tgttagagat atgaatggcc atgcgttacc
      421 tttaacaaaa gatggcaatt tttatcaaac gaatgtagat gcaaatggta ttaatcatgg
      481 tggtagtgaa atggtgcaaa ataaaacagg tcatatgagt caacaaggcc atatgaatca
      541 gaacacacac atgaaccaac agccacacat gcaacaaggt catatgcaat catcaaacca
      601 tcaaatgatg agtccaaaag caaatatgca ttcatcaaat catcaaatga accaaagtaa
      661 caaaaaagtt ttaccagctg ctggtgaaag tatgacatca agtattctta ctgcaagtat
      721 tgccgcacta ctattagtat ctgggttatt cttagcattt agacgacgtt caacaaataa
      781 ataaacataa tacgattaat aatagaaaaa tcgtgtgatt atctgagcga gcctaggaca
      841 taaatcaatg tcctaggctc gctaatgtta tattggcagt agttgactga atgaaattgc
      901 gcttgtaaca agcttttcca tttcttacca actccctaaa tagtcatcaa aaaatttctt
      961 atattttaat aattttaata atccgattgt cttatacgtg tcagtgttaa ttcagagatt
     1021 tcctgtggaa tataccactt attaatcata attggataag gtgtttgtgc gtacaatgtt
     1081 tcaataatca gccaacaatg tgtatcacca tcaaacacgt gactatgatt tttgaagtgg
     1141 ggcgctttgg taatagacat ttttaaatct gattgatatg cattgctata aatcgtttgc
     1201 tcaacgaatg tcttcatgtc gtcttcgttt tgtgtattca ctttaaatgt gtcaatgaca
     1261 tttaacggta taaaggtaaa gcaaaatgca tcagcttgct tagaatgatt gtcctttttt
     1321 tgataatagc gttccattgc aatgacggca gaaggatggt ttgcaaacaa atgatttgta
     1381 tattcacttt ctaaatcaac acgataatta attgatgaca tagatacgcg agctagcaat
     1441 atttgatcaa gtggatgctt aaattgatcc atacttgaag cgtgttgggc atttgtttgt
     1501 ggaataacaa agtgtccctt ccctcttgta ctctctacga tgccatcttc ggctaacaat
     1561 tttatagctt ggcgcaaagt catacgactg acatcaaagc gcgcacaaag ttccttttca
     1621 gtaggtaatg catggccact cggatatttt cctatttgaa tttctttata taacgtatta
     1681 taaatcgtta aaaattttgg ttgtgtttgc gtcacgtaga caacctccat aaagttactt
     1741 aatcactctc atcatacaat aatttttact cgaattggaa aaattataaa aattaaatat
     1801 agataggctt tgaaaattag ttttatacaa ggttagtagc tgtaactgta aaatgttctt
     1861 aatattgtca aaatgtaatg cttgaaagcg cttttaaaaa atattattat atacatggtt
     1921 agacaaatag acaaatcact atacaaatat tgggaggaat attttatgaa atcaacacca
     1981 cacattaaac caatgaatga cgtcgaaatt gcagaaacgg ttctattgcc aggagatccg
     2041 ttaagagcta agttcattgc agaaacttat ttggatgatg tggaacagtt caatacagtg
     2101 cgaaacatgt ttggttttac cggaacatat aaaggtaaaa aagtttctgt catgggttca
     2161 ggtatgggta tgccatctat tggcatttac tcttatgaat taattcatac atttggttgt
     2221 aaaaaattaa ttcgcgttgg ttcttgtggc gcgatgcaag aaaacattga tttatatgat
     2281 gtgattattg cacaaggtgc ctctactgat tcaaattacg ttcaacaata tcaattacca
     2341 ggtcattttg cgccaattgc ttcttatcaa ttattagaaa aagcagttgg aacagcacgt
     2401 gacaaaggtg tacgtcatca tgtaggtaat gtgttatcaa gtgatatttt ctataacgcg
     2461 gatacaacag cgagtgaacg ttggatgcgt atgggtattt taggtgtaga aatggaatca
     2521 gctgcattat acatgaatgc aatttacgct ggtgtcgaag cattaggtgt gttcacagtg
     2581 agcgatcatt taattcatga aacgtcaaca acacctgagg aaagggaacg tgcatttaca
     2641 gatatgattg aaattgcact gtcattggtt tagataatta tgaatgttga atattctaaa
     2701 ataaagaaag cagtacctat tttattattc ttatttgtat tcagtttggt tatagacaac
     2761 tcatttaaat tgatttctgt agccattgct gatgacttaa acatttctgt aacgacagta
     2821 agttggcaag cgacattagc cggtttagta attggtattg gcgctgtagt atacgcttca
     2881 ttatctgatg ccattagtat acgcacacta tttatttatg gcgtgatatt aatcattatc
     2941 ggatcaatta ttggttacat tttccaacat caattcgcat tacttttagt tggacgtatt
     3001 attcaaactg ccggtttagc cgctgcagag acattatatg tgatatatgt tgcaaagtat
     3061 ctttctaaag aggaccagaa gacttacctt ggcttaagta cgagcagtta ttccttgtca
     3121 ttagttatcg gtacattatc aggtggattt atttctacgt atttacactg gacaaatatg
     3181 tttttaattg cattaatcgt agtatttacg ttgccattcc tatttaaatt attaccaaaa
     3241 gaaaataata cgaataaagc tcatttagat tttgttggct taattctagt ggcaactatt
     3301 gctacaacag tcatgctgtt tattacgaac tttaattggt tatatatgat tggtgcctta
     3361 attgcgatta tcgtttttgc gctatatatt aaaaatgcgc aacgtccatt agtaaataaa
     3421 tcatttttcc aaaataaacg ttatgcttca tttttattta tagtatttgt aatgtatgct
     3481 atccaattgg gttatatttt tacgttccca ttcataatgg agcaaattta tcatctgcaa
     3541 ctagacacaa catcactgtt attagtaccg ggttatatag tagcagtcat cgttggtgca
     3601 ctaagtggta aaatcggcga atatctgaat tcaaaacaag cgattatcac agcaattatt
     3661 ttaatcgcac tgagcttgat tttacctgca tttgcagtag gtaatcacat ttcaatcttc
     3721 gtcatttcta tgatattctt tgcaggtagc tttgctttaa tgtatgcacc tttacttaac
     3781 gaagccatta aaacaataga tcttaatatg acaggtgtgg ctattggttt ttataattta
     3841 attattaatg tggcggtatc tgtaggtatt gcgattgctg cggctctaat cgattttaaa
     3901 gcattaaatt tcccaggcaa tgatgcatta agttcacatt tcggtattat tttaattatt
     3961 ttaggtttaa tgagtattgt cggattagtt ttattcgtca gcttaaatcg ttggacacaa
     4021 tctgaaaaat aaatagatat aaattcgcga gatatattcg tatttatagt aaaattaaat
     4081 aaagagatta tataacacga ggagtagtaa gtatgaaatt tgagaaatat atagatcaca
     4141 ctttattgaa gcctgagtca acacgtacgc aaa
//
LOCUS       DAMZKC010000425         2226 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.guided.257, whole genome shotgun sequence.
ACCESSION   DAMZKC010000425 DAMZKC010000000
VERSION     DAMZKC010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.guided.257"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            342..1298
                     /locus_tag="POB55_002909"
     CDS             342..1298
                     /locus_tag="POB55_002909"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDI6501790.1"
                     /translation="MLNSFDAAYHSLCEEVLEIGNTRNDRTNTGTISKFGHQLRFDLS
                     KGFPLLTTKKVSFKLVATELLWFIKGDTNIQYLLKYNNNIWNEWAFENYIKSDEYNGP
                     DMTDFGHRALSDPEFNEQYKEQMKQFKQRILEDDTFAKQFGDLGNVYGKQWRDWVDKD
                     GNHFDQLKTVIEQIKHNPDSRRHIVSAWNPTEIDTMALPPCHTMFQFYVQDGKLSCQL
                     YQRSADIFLGVPFNIASYALLTHLIAKECGLEVGEFVHTFGDAHIYSNHIDAIQTQLA
                     RESFNPPTLKINSDKSIFDINYEDLEIVDYESHPAIKAPIAV"
     gene            1498..1977
                     /locus_tag="POB55_002910"
     CDS             1498..1977
                     /locus_tag="POB55_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDI6501791.1"
                     /translation="MTLSILVAHDLQRVIGFENQLPWHLPNDLKHVKKLSTGHTLVMG
                     RKTFESIGKPLPNRRNVVLTSDTSFNVVGVDVIHSIEDIYQLPGHVFIFGGQILFEEM
                     IDKVDDMYITVIEGKFRGDTFFPPYTFEDWEVASSVEGKLDEKNTIPHTFLHLIRKK"
     gene            1992..>2226
                     /gene="fakB2"
                     /locus_tag="POB55_002911"
     CDS             1992..>2226
                     /gene="fakB2"
                     /locus_tag="POB55_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase binding subunit FakB2"
                     /protein_id="HDI6501792.1"
                     /translation="MTKQIIVTDSTSDLSKEYLEANNIHVIPLSLTIEGASYVDQVDI
                     TSEEFINHIENDEDVKTSQPAIGEFISAYEELGK"
BASE COUNT          827 a          328 c          366 g          705 t
ORIGIN      
        1 attctatttg ttaaataaag tccgttttta ccaactatat tttctagaaa tttaactgtt
       61 ttaataggac atcaaacata atattcaaat catgtgttaa cctctttttt taaatttttt
      121 agcattaaag ttatagattt gggtaaacaa ttaccaattg gaaacatata tcacgttacg
      181 atgtggtagg tacttaatca gcattttata aataaagtaa cggaattcat gatattaata
      241 tcatattcct aaaatgagtg ataacaaaat gctacataaa attaagttat atcaaactaa
      301 atatacatac tataaataat gaaaatgagg tgttatcgca tatgttgaat tcatttgatg
      361 cagcatatca cagtctttgt gaagaagttt tagaaatagg aaatacacga aatgatcgca
      421 caaatacagg tacgatttcg aaatttggtc atcaacttcg ctttgactta tctaaaggat
      481 ttccactatt aacgacaaag aaagtttctt ttaaattagt agcaaccgaa ttattatggt
      541 tcattaaagg agatacaaac atccaatact tattaaaata taataataat atttggaacg
      601 aatgggcttt tgaaaattat ataaaatcag acgagtataa cggtccagat atgacagatt
      661 tcgggcatcg tgcattgagt gatcctgaat ttaacgaaca atataaagaa caaatgaaac
      721 aatttaagca acgtattctt gaagatgata cttttgcgaa gcaattcggt gatttaggaa
      781 atgtttatgg taaacaatgg cgagattggg ttgataaaga tggtaatcat tttgatcaac
      841 ttaaaacagt aattgaacaa attaagcata atccagattc aagacgacac atcgtatctg
      901 catggaatcc aacagaaatt gatacaatgg cacttccgcc ttgtcatacc atgttccagt
      961 tttatgtcca agatggtaag ttaagttgcc agttatacca acgtagcgca gatatctttt
     1021 taggtgtgcc atttaatatc gcaagctacg ctttattgac acaccttatt gccaaagaat
     1081 gtggacttga agtaggagaa tttgtgcata catttggaga tgcacatatt tattcaaatc
     1141 atattgatgc gattcaaaca caattagcac gtgaaagctt caatcctcca acattaaaaa
     1201 ttaacagtga caagtctatt ttcgacataa attatgaaga tttggaaatt gttgactatg
     1261 aatcacatcc agcaataaaa gctccaatag cagtgtagtc attgcatagt tagctaacca
     1321 tatagacatc aaaatgacat catagtattt tcaagtgcaa aaaagtactt ttttgtgtta
     1381 aacgttttca taaattatgc aaaatcatta tttctatcac actttatgat aaaaattgtg
     1441 ttaaattaaa gataacttag taataaaaaa tgaaatgata gaagaaggag gataattatg
     1501 actttatcca ttctagttgc acatgacttg caacgagtaa ttggttttga aaatcaatta
     1561 ccttggcacc taccaaatga tttgaaacat gttaaaaaat tatcaacagg tcatacttta
     1621 gtaatgggtc gtaagacatt tgaatcgatt ggtaaaccac taccgaatcg tcgaaatgtt
     1681 gtacttactt cagatacaag tttcaacgta gtgggcgttg atgtaattca ctctattgaa
     1741 gatatttacc aactaccggg ccatgttttc atatttggag ggcaaatatt atttgaagaa
     1801 atgattgata aagtggacga catgtatatt actgttattg aaggtaaatt ccgtggtgat
     1861 acgttctttc caccttatac atttgaagac tgggaagttg cctcttcagt tgaaggtaaa
     1921 ctagatgaga aaaatacaat tccacatacc tttctacatt taattcgtaa aaaataaggg
     1981 ggaaaacgac catgacaaaa cagattatag taacagactc aacatccgat ttatctaaag
     2041 aatacttaga agcaaacaac attcatgtaa ttcctttaag tttaactatt gaaggagctt
     2101 catacgttga ccaagtagat attacatcag aagaatttat taatcatatt gaaaatgatg
     2161 aagatgtaaa gacaagtcag ccagccatag gtgaatttat atctgcttat gaagaactag
     2221 gaaaag
//
LOCUS       DAMZKC010000426         1254 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Staphylococcus aureus isolate DMG1700949
            SAMN08111885-rid16878373.guided.317, whole genome shotgun sequence.
ACCESSION   DAMZKC010000426 DAMZKC010000000
VERSION     DAMZKC010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08111885
            Sequence Read Archive: SRR6332162
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591392.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591392.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:48:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,912
            CDSs (total)                      :: 2,847
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 39 of 55
            Pseudo Genes (incomplete)         :: 17 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 12 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1254
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08111885-rid16878373.guided.317"
                     /isolate="DMG1700949"
                     /isolation_source="Human clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="New Zealand: Porirua"
                     /lat_lon="41.1474 S 174.8389 E"
                     /collection_date="2014"
                     /collected_by="Insitiute of Environmental Science and
                     Research (ESR)"
     gene            158..796
                     /locus_tag="POB55_002912"
     CDS             158..796
                     /locus_tag="POB55_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fusidic acid resistance EF-G-binding protein
                     FusC"
                     /protein_id="HDI6501793.1"
                     /translation="MNKIEVYKFVKVKQLVYQLIKLYRTNDMNSHKTQKDFLLNEIND
                     IFKEKDIDISDFITSIDDVKLTKKKAEHLLNELKVYIQDFEIPSSSQLEKIFRKVKKL
                     KRPDINLIDTKEISYLGWNDNSSNRKYIVYKNLDDKFEGIYGEISPNKVKGFCKICNQ
                     ESDTSLFLNKTKHNKSSGTYTKKGDYICYDSFKCNQNLDDINNLYEFIVKIK"
BASE COUNT          541 a          140 c          188 g          385 t
ORIGIN      
        1 gatagtatgg aagattttta taggagctga ttataatgcg acatataaat atgtcataca
       61 tcatacgcta tagtactcta actcaaagat aagaagtact tagttatgaa tagcattcat
      121 ttcacaaagt acttcaacaa aaatggagga atatgaaatg aataaaatag aagtgtataa
      181 gtttgttaaa gtaaagcagt tagtatatca attgattaag ttatatcgta caaacgatat
      241 gaattcccat aaaacacaaa aagatttttt actaaatgaa attaatgata tctttaaaga
      301 aaaagatatt gatatctcgg actttattac atcgattgac gatgtaaaat taactaagaa
      361 aaaagcagaa catcttttaa atgaattaaa agtgtacatc caagattttg aaataccttc
      421 atcaagtcaa ctggagaaaa tttttcgtaa agtaaaaaaa ttaaagagac cagatataaa
      481 tttaattgat acaaaagaaa tttcatattt aggatggaat gataattctt ctaaccgaaa
      541 atatatcgtt tataaaaatt tagatgataa attcgaaggt atatatggcg aaatttcacc
      601 aaataaagta aaaggattct gtaaaatttg taatcaggaa tctgatacat cactctttct
      661 caataaaact aaacataata agagtagtgg aacatatact aaaaaaggag attacatttg
      721 ttatgacagt tttaaatgta atcagaacct agatgatata aataatcttt acgaatttat
      781 tgttaaaata aaatagatcc aaacagccct gatctttaga actaatgatc agggttgtta
      841 tattttgtac ctcactctta atcgatacgc tgaaagtccc tataaataga acgtacgttc
      901 cgtttgtagt ataatgacaa aaatattaaa ttagaggatt aaaaatggga ttataatttt
      961 ttaatgtacc aaactgaaaa aggctaaata aagataaata taagattaga aaaaactgta
     1021 tggattaggc aatatttata tataatgaaa tgaattaaca gaaaatcgaa ttatccggaa
     1081 aaaccgtact gttcgattgg aagggaatag agaaggtgaa aagaattaac ttcaattatc
     1141 atcttatttc tgaaaaaaga tatgaaaaac ggacgcaaga cactttaaat attagtaaca
     1201 acgtaaagtg aataaaatct atattaaggc atcgtgatga atttaatata attg
//
